Survival of human parathyroid tissue xenotransplanted in nude mice after 9 to 55 months' cryopreservation.
Survival of human parathyroid tissue xenotransplanted after cryopreservation was studied. Peroperative biopsies from 26 patients were cryopreserved and xenotransplanted into nude mice after 9 to 55 months. At 8 to 12 weeks after transplantation, the morphology of the transplanted tissue was compared to that of the original tissue after thawing and before transplantation. Morphologically viable tissue was observed in 20 out of 26 nude mice (77%). Based on the morphological appearance, the parathyroid transplants were arranged into four "quality" groups. No correlation existed between the quality of the transplants and duration of storage, or between the age and sex of the patients. There was no correlation between initial clinical diagnosis or histopathological patterns (primary, secondary and tertiary hyperplasia [n=16], adenoma [n=9], one case undetermined) and transplant survival. After thawing and transplantation, all parathyroid grafts, except one, were morphologically either of the same or somewhat lower quality.
Smeds
S
S
The Department of Biomedicine and Surgery, University Hospital, Linköping, Sweden.
Trulsson
L
L
Garovoy
M
M
Gumbert
M
M
Clark
O H
OH
D000236:Adenoma; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015925:Cryopreservation; D005260:Female; D006085:Graft Survival; D006801:Humans; D006965:Hyperplasia; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D010280:Parathyroid Glands / Q000473:pathology / Q000637:transplantation*; D010282:Parathyroid Neoplasms; D014183:Transplantation, Heterologous