Validation of animal experiments on ciliary function in vitro. II. The influence of absorption enhancers, preservatives and physiologic saline.
Ciliary beat frequency (CBF) is one of the most important parameters of mucociliary clearance. Previously, we demonstrated that mucosa from chicken embryo trachea is a good substitute for human ciliated epithelium to study the effects on CBF of substances that are used clinically. In this study, we examined the effect on CBF of four excipients for nasal drug formulations: the absorption enhancers methylated beta-cyclodextrin 2% and sodium taurodihydrofusidate 1%, the preservative benzalkonium chloride 0.01%, and physiologic saline. We also examined the effect on CBF of the cryopreservative dimethyl sulfoxide, which is used to protect ciliated epithelium prior to storage in liquid nitrogen. Results obtained with chicken embryo trachea were compared with those of cryopreserved human mucosa taken from the sphenoidal sinus. For all of the substances tested, the effects on CBF of chicken material were comparable to those measured on human material. Benzalkonium chloride had a stronger ciliostatic effect on human tissue. After 60 min, however, the effect of that substance on CBF was similar in both tissues. We conclude that chicken embryo trachea can be used as a substitute for human ciliated mucosa when studying ciliary activity in vitro.
Boek
W M
WM
Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.
Romeijn
S G
SG
Graamans
K
K
Verhoef
J C
JC
Merkus
F W
FW
Huizing
E H
EH
D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology; D002642:Chick Embryo; D003505:Cyclodextrins / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005079:Excipients / Q000494:pharmacology; D006801:Humans; D009079:Mucociliary Clearance / Q000187:drug effects*; D009092:Mucous Membrane / Q000187:drug effects; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects; D047392:beta-Cyclodextrins
D001548:Benzalkonium Compounds; D003505:Cyclodextrins; D005079:Excipients; D011310:Preservatives, Pharmaceutical; D047392:beta-Cyclodextrins; D012965:Sodium Chloride; C031215:betadex; D004121:Dimethyl Sulfoxide