Cholesterol hydroperoxides in erythrocyte membranes of alcoholic patients.
Evidence for the presence of 5alpha-hydroperoxycholest-6-en-3beta-ol (cholesterol 5alpha-hydroperoxide, Ch 5alpha-OOH) and 7alpha- and 7beta-hydroperoxycholest-5-en-3beta-ols (cholesterol 7-hydroperoxides: Ch 7alpha-OOH and Ch 7beta-OOH, respectively) in human erythrocyte membrane was found. Blood samples were collected from alcoholic patients and healthy volunteers (controls), and their cholesterol hydroperoxides were analyzed by high-performance liquid chromatography postcolumn chemiluminescence and roughly identified by liquid chromatography-mass spectrometry. Ch 7alpha-OOH and Ch 7beta-OOH were present in each sample, being significantly higher in alcoholic samples than in control samples. Ch 5alpha-OOH was present in some alcoholic samples, but not in the control ones. The accumulation of cholesterol hydroperoxides suggests enhanced lipid peroxidation by active oxygen species and/or a reduced elimination system for lipid peroxide in alcoholic patients.
Adachi
J
J
Department of Legal Medicine, Kobe University School of Medicine, Japan.
Asano
M
M
Naito
T
T
Ueno
Y
Y
Imamichi
H
H
Tatsuno
Y
Y
D000328:Adult; D000437:Alcoholism / Q000097:blood*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged
C041011:cholesterol 7-hydroperoxide; C002843:cholesterol hydroperoxide; D002784:Cholesterol