title: Selective killing of osteosarcoma cells in culture after retroviral mediated gene transfer of a herpes simplex thymidine kinase suicide gene.
abstract: As conventional therapy of osteosarcoma is aggressive and its success relatively poor, notably in cases relapse, we investigated the therapeutic effect of retroviral herpes simplex thymidine kinase (HSV1-TK) gene transfer into a human osteosarcoma cell line. Transfected target cells are sensitive to ganciclovir (GCV) (IC50 0.1 microM), and a potent bystander effect, by which cell death can be induced in HSV1-TK negative dividing cells located in the vicinity of HSV1-TK positive ones, is demonstrated. This is significant for clinical applications as no available gene transfer method can achieve 100% transduction to target cells.
authors.affiliation: Laboratoire de Biochimie et de Biologie Moléculaire, Faculté de Médecine, Limoges, France.
authors.forename: L
authors.initials: L
authors.lastname: Grossin
authors.forename: J L
authors.initials: JL
authors.lastname: Charissoux
authors.forename: M P
authors.initials: MP
authors.lastname: Teissier
authors.forename: M
authors.initials: M
authors.lastname: Rigaud
mesh_terms: D015774:Ganciclovir / Q000494:pharmacology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000032:analysis; D012190:Retroviridae / Q000235:genetics*; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D013937:Thymidine Kinase; D015774:Ganciclovir