title: A catalytically active complex formed from the recombinant dI protein of Rhodospirillum rubrum transhydrogenase, and the recombinant dIII protein of the human enzyme.
abstract: Transhydrogenase is a proton pump. It has three components: dI and dIII protrude from the membrane and contain the binding sites for NAD(H) and NADP(H), respectively, and dII spans the membrane. We have expressed dIII from Homo sapiens transhydrogenase (hsdIII) in Escherichia coli. The purified protein was associated with stoichiometric amounts of NADP(H) bound to the catalytic site. The NADP+ and NADPH were released only slowly from the protein, supporting the suggestion that nucleotide-binding by dIII is regulated by the membrane-spanning dII. HsdIII formed a catalytically active complex with recombinant dI from Rhodospirillum rubrum (rrdI), even in the absence of dII. The rates of forward and reverse transhydrogenation catalysed by this complex are probably limited by slow release from dIII of NADPH and NADP+, respectively. The hybrid complex also catalysed high rates of 'cyclic' transhydrogenation, indicating that hydride transfer, and exchange of nucleotides with dI, are rapid. Stopped-flow experiments revealed a rapid, monoexponential, single-turnover burst of reverse transhydrogenation in pre-steady-state. The apparent first-order rate constant of the burst increased with the concentration of rrdI. A deuterium isotope effect (kH/kD approximately 2 at 27 degrees C) was observed when [4B-1H]NADPH was replaced with [4B-2H]NADPH. The characteristics of the burst of transhydrogenation with rrdI:hsdIII differed from those previously reported for rrdI:rrdIII (J.D. Venning et al., Eur. J. Biochem. 257 (1998) 202-209), but the differences are readily explained by a greater dissociation constant of the hybrid complex. The steady-state rate of reverse transhydrogenation by the rrdI:hsdIII complex was almost independent of pH, but there was a single apparent pKa ( approximately 9.1) associated with the cyclic reaction. The reactions of the dI:dIII complex probably proceed independently of those protonation/deprotonation reactions which, in the complete enzyme, are associated with H+ translocation.
authors.affiliation: School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Peake
authors.forename: J D
authors.initials: JD
authors.lastname: Venning
authors.forename: J B
authors.initials: JB
authors.lastname: Jackson
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D003001:Cloning, Molecular; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000201:enzymology; D009249:NADP / Q000737:chemistry; D009250:NADP Transhydrogenases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012247:Rhodospirillum rubrum / Q000201:enzymology* / Q000235:genetics; D013050:Spectrometry, Fluorescence
chemical_list: D009249:NADP; D009250:NADP Transhydrogenases