title: Prolactin receptor expression in the testis of the ram: localisation, functional activation and the influence of gonadotrophins.
abstract: The present study investigated the pattern and site of expression of the prolactin receptor gene in the testis of the seasonally breeding Soay sheep. In experiment 1, Northern blot analysis confirmed expression of the prolactin receptor gene in the testis which was encoded by RNA transcripts of approximately 3.6, 11.2, 12.6, and 14.1 kb. In situ hybridisation localised expression of the receptor within the interstitial and seminiferous tubule compartments of the testis and immunohistochemistry localised expression of the receptor to Leydig cells and to pachytene spermatocytes, round and elongating spermatids. In experiment 2, phosphorylation of Jak2, Stat1 and Stat5 proteins in response to prolactin was investigated by Western blotting following incubation of testicular samples with 100 ng/ml ovine prolactin. Jak2 and Stat1 phosphorylation were induced by prolactin within 10 min and Stat5 within 30 min. In experiment 3, intact and hypothalamo-pituitary disconnected (HPD) rams were transferred from a short to a long day photoperiod regimen for a period of 8 weeks. By week 8, testicular diameter had declined in intact rams (52.71+/-1.06 cm vs. 48.00+/-0.49 cm for weeks 1 and 8 after transfer respectively, P<0.01, n = 3) and increased in HPD rams (27.00+/-0.45 cm and 29.66+/-0.99 cm for weeks 1 and 8 after transfer respectively, P<0.05, n = 3). RT-PCR using RNA extracted from intact and HPD rams confirmed expression of the prolactin receptor in the testis of both groups. Immunohistochemistry localised prolactin receptor expression in Leydig cells and in pachytene spermatocytes, round and elongating spermatids of intact sheep testis. In HPD rams, prolactin receptor expression was localised in Leydig cells and germ cells which were arrested predominantly at the pachytene spermatocyte stage. These data demonstrate expression of functional prolactin receptors in the testis of Soay rams. The site and pattern of expression of the receptor gene suggest a role for prolactin in the regulation of steroidogenesis and spermatogenesis.
authors.affiliation: MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK. h.jabbour@ed-rbu.mrc.ac.uk
authors.forename: H N
authors.initials: HN
authors.lastname: Jabbour
authors.forename: G A
authors.initials: GA
authors.lastname: Lincoln
mesh_terms: D000818:Animals; D017931:DNA Primers; D005640:Follicle Stimulating Hormone / Q000097:blood; D015870:Gene Expression; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D011388:Prolactin / Q000032:analysis / Q000097:blood / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D011388:Prolactin; D005640:Follicle Stimulating Hormone