Alteration in diastolic function following cardiac cryopreservation at subzero temperatures.
BACKGROUND
We have studied the alterations produced in the diastolic function of the left ventricle after applying a protocol of cryopreservation at subzero temperatures.


METHODS
Isolated rabbit hearts and 5% polyethylene glycol (PM 4000) as the cryoprotective agent were used for the study.


CONCLUSIONS
Following cryopreservation we found a statistically significant increase in systolic function. However, the diastolic function shows worsening, with a statistically significant increase (p < 0.05) in mean stiffness, decrease in differential stiffness, (p < 0.05) and upward and leftward displacement of the diastolic pressure-volume curve.
Gutiérrez-Carretero
E
E
Virgen del Rocio Hospital, Seville, Spain.
Bello-Puentes
R
R
Borrego-Domínguez
J M
JM
Hernández-Fernández
A
A
Muñoz-Garcia
J
J
Ordóñez-Fernández
A
A
D000818:Animals; D002306:Cardiac Volume / Q000502:physiology; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000627:therapeutic use; D003971:Diastole / Q000502:physiology*; D004548:Elasticity; D005615:Freezing; D005947:Glucose / Q000627:therapeutic use; D016027:Heart Transplantation / Q000502:physiology*; D019188:Organ Preservation Solutions / Q000627:therapeutic use; D011092:Polyethylene Glycols / Q000627:therapeutic use; D011817:Rabbits; D013501:Surface-Active Agents / Q000627:therapeutic use; D013599:Systole / Q000502:physiology; D014325:Tromethamine / Q000627:therapeutic use; D016277:Ventricular Function, Left / Q000502:physiology; D017725:Ventricular Pressure / Q000502:physiology
D003451:Cryoprotective Agents; C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D013501:Surface-Active Agents; D014325:Tromethamine; D011092:Polyethylene Glycols; D005947:Glucose