Detection of the presence of soya protein in milk powder by sodium dodecyl sulfate capillary electrophoresis.
Two different commercial kits for sodium dodecyl sulfate capillary electrophoresis (SDS-CE) were evaluated for the detection of the presence of soya protein in milk powder. The results obtained showed that SDS-CE allowed the separation of the basic subunits of glycinin and the alpha and alpha' subunits of beta-conglycinin from the main milk protein peaks. However, a detection limit lower than 10% (w/w) of soya protein in total protein could not be achieved. The use of a tetraborate-EDTA sample treatment minimized interferences from milk proteins, allowing the detection of at least 1% (w/w) of soya protein in total protein. The addition of soya protein hydrolysates could not be determined using SDS-CE.
López-Tapia
J
J
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
García-Risco
M R
MR
Manso
M A
MA
López-Fandiño
R
R
D002138:Calibration; D003611:Dairy Products / Q000032:analysis*; D004492:Edetic Acid; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D008894:Milk Proteins / Q000032:analysis; D008970:Molecular Weight; D030262:Soybean Proteins / Q000032:analysis*
D008894:Milk Proteins; D030262:Soybean Proteins; D004492:Edetic Acid