title: Thioredoxin peroxidase in the Cyanobacterium Synechocystis sp. PCC 6803.
abstract: The amino acid sequence deduced from the open reading frame designated sll0755 in Synechocystis sp. PCC 6803 is similar to the amino acid sequences of thioredoxin peroxidases from other organisms. In the present study, we found that a recombinant SLL0755 protein that was expressed in Escherichia coli was able to reduce H2O2 and tertiary butyl hydroperoxide with thioredoxin from E. coli as the electron donor. Targeted disruption of open reading frame sll0755 in Synechocystis sp. PCC 6803 cells completely eliminated the H2O2-dependent and tertiary butyl hydroperoxide-dependent photosynthetic evolution of oxygen and the electron flow in photosystem II. These results indicate that the product of open reading frame sll0755 is a thioredoxin peroxidase whose activities are coupled to the photosynthetic electron transport system in Synechocystis sp. PCC 6803.
authors.affiliation: Plant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth, Kizu, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: C
authors.initials: C
authors.lastname: Miyake
authors.forename: K J
authors.initials: KJ
authors.lastname: Dietz
authors.forename: K
authors.initials: K
authors.lastname: Tomizawa
authors.forename: N
authors.initials: N
authors.lastname: Murata
authors.forename: A
authors.initials: A
authors.lastname: Yokota
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004579:Electron Transport; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D016366:Open Reading Frames; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism*; D054464:Peroxiredoxins; D010788:Photosynthesis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D020122:tert-Butylhydroperoxide / Q000378:metabolism
chemical_list: D017931:DNA Primers; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054464:Peroxiredoxins