title: [Sensitivity to selected beta-lactam antibiotics of clinical strains of Escherichia coli and Klebsiella pneumoniae].
abstract: The studies aimed at analysing the resistance to some beta-lactam antibiotics among E. coli and K. pneumoniae clinical isolates and at evaluating. The extended spectrum of beta-lactamases (ESBL) production in the isolates. The analysis included 137 E. coli strains and 52 K. pneumoniae strains, isolated from hospitalized patients and out-patients treated in the first trimester of 1998. The strains were identified using the ATB computer system. Antibiotic sensitivity of the isolates was determined by disc-diffusion tests. ESBL production capacity of E. coli and K. pneumoniae strains was estimated by double-disc and ATB BLSA tests. Most of the analysed E. coli strains were found to exhibit significant sensitivity to compound penicillin preparations containing beta-lactam inhibitor (Augmentin, Tazocin) and to the third generation cefalosporins, in contrast, K. pneumoniae strains much more frequently were resistant to the drugs. Among the obtained isolates, 3 (2.2%) E. coli strains and 21 (40.4%) K. pneumoniae strains produced ESBL but all the isolates proved sensitive to imipenem. In evaluation of ESBL production-detecting tests, the double-disc test was found to be more reliable than ATB BLSA test.
authors.affiliation: Zakład Mikrobiologii Lekarskiej A.M. im. Karola Marcinkowskiego w Poznaniu.
authors.forename: E
authors.initials: E
authors.lastname: Andrzejewska
authors.forename: A
authors.initials: A
authors.lastname: Szkaradkiewicz
authors.forename: M
authors.initials: M
authors.lastname: Kaniasty
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D001826:Body Fluids / Q000382:microbiology; D002511:Cephalosporins / Q000494:pharmacology; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D015378:Imipenem