A capture enzyme-linked immunosorbent assay for species-specific detection of Bothrops venoms.
A direct sandwich enzyme-linked immunosorbent assay (ELISA), employing affinity purified antivenom antibodies specifically recognizing the homologous venom, was developed for species-specific detection of bothropic venom. The method is based on a two-step affinity purification of the specific antibodies. A species monovalent antivenom is adsorbed onto a venom adsorbent containing heterologous venoms from the Bothrops, Crotalus and Lachesis genera. The species-specific antibodies obtained, are then adsorbed onto a second venom adsorbent containing only the homologous venom for the removal of non antivenom antibodies. Venom concentrations of 0.1 and 1,000 ng/ml were specifically identified for Bothrops jararacussu and B. alternatus venom respectively.
Heneine
L G
LG
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Araújo dos Santos
M R
MR
Dutra de Carvalho
A
A
da Silva Gontijo
S
S
D000818:Animals; D000918:Antibody Specificity; D017837:Bothrops; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D007074:Immunoglobulin G; D051379:Mice; D012680:Sensitivity and Specificity; D013045:Species Specificity
D003435:Crotalid Venoms; D007074:Immunoglobulin G