Detection of avian adenovirus by polymerase chain reaction.
An avian adenovirus-specific polymerase chain reaction was developed. The origin of primers was from the DNA sequence data of the chicken embryo lethal orphan avian adenovirus virus genome. An avian adenovirus-specific 421-bp DNA product was amplified by these primers from group I of adenovirus containing 12 serotypes and serotypes of adenovirus from group II and group III. The adenovirus-specific DNA product was also amplified from the 19 field isolates of avian adenoviruses but not from the mammalian adenovirus and other avian pathogenic viruses and bacteria. As little as 1 fg of avian adenovirus DNA was detected by gel electrophoresis and Southern blot analysis.
Xie
Z
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Department of Pathobiology, College of Agriculture and Natural Resources, University of Connecticut, Storrs 06269-3089, USA.
Fadl
A A
AA
Girshick
T
T
Khan
M I
MI
D000818:Animals; D000259:Aviadenovirus / Q000302:isolation & purification*; D002642:Chick Embryo; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity
D004279:DNA, Viral