title: The time-dependent clearance of virulent Treponema pallidum in susceptible and resistant strains of guinea pigs is significantly different.
abstract: The kinetics of clearance of Treponema pallidum spp. pallidum Nichols from skin and testes of susceptible C4-deficient (C4D) and -resistant Albany (Alb) strains of guinea pigs (gps) was evaluated using the polymerase chain reaction (PCR) and the rabbit infectivity test (RIT). For each strain there were two groups of animals, one infected with virulent T. pallidum (TP) and one control injected with heat-killed treponemes (HKTP). The kinetic studies and their statistical analysis showed that in the C4D strain the microbial clearance in both tissues was significantly slower (p < 0.005) and still incomplete at 3 months after infection. In the Alb strain the clearance was faster and apparently completed within a month. A greater permissiveness in bacterial growth in C4D compared to Alb appears to be one critical factor determining the different rate of local elimination after primary infection. In both strains there was some correlation between the severity and duration of cutaneous lesions and the local persistence of viable organisms. This correlation was not observed in testes. These studies suggest a genetic basis for the strain-specific susceptibility and resistance phenotypes in the pathogenesis of syphilis.
authors.affiliation: David Axelrod Institute, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-2002, USA.
authors.forename: V
authors.initials: V
authors.lastname: Wicher
authors.forename: K
authors.initials: K
authors.lastname: Wicher
authors.forename: F
authors.initials: F
authors.lastname: Abbruscato
authors.forename: I
authors.initials: I
authors.lastname: Auger
authors.forename: U
authors.initials: U
authors.lastname: Rudofsky
mesh_terms: D000818:Animals; D003181:Complement C4 / Q000172:deficiency; D006168:Guinea Pigs; D008297:Male; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012867:Skin / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013587:Syphilis / Q000276:immunology / Q000382:microbiology; D013737:Testis / Q000382:microbiology; D013997:Time Factors; D014210:Treponema pallidum / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D003181:Complement C4