title: Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA).
abstract: Xanthine oxidase was purified from human milk and used to immunise rabbits. A competitive immunoenzymatic assay with purified enzyme and rabbit antiserum was optimised to measure xanthine oxidase in human serum, the lowest detectable amount being 0.03 pmol of enzymatic protein. Thus, the test (i) is sensitive enough to determine xanthine oxidase in human serum, being more sensitive than the spectrophotometric method, (ii) it is more convenient for clinical laboratories than other sensitive tests and (iii) it has the advantage over the enzyme activity-based assays of also detecting inactive enzyme molecules. A competitive enzyme-linked immunosorbent assay (ELISA) was used to measure the serum xanthine oxidase level in healthy donors and in patients with liver diseases, and it was found that any concentration below 1 mg/L is in the normal range.
authors.affiliation: Department of Experimental Pathology, University of Bologna, Italy. mgbatt@alma.unibo.it
authors.forename: M G
authors.initials: MG
authors.lastname: Battelli
authors.forename: A
authors.initials: A
authors.lastname: Abbondanza
authors.forename: S
authors.initials: S
authors.lastname: Musiani
authors.forename: L
authors.initials: L
authors.lastname: Buonamici
authors.forename: P
authors.initials: P
authors.lastname: Strocchi
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: L
authors.initials: L
authors.lastname: Gramantieri
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D008107:Liver Diseases / Q000097:blood / Q000201:enzymology; D008892:Milk / Q000201:enzymology; D014969:Xanthine Oxidase / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D007106:Immune Sera; D014969:Xanthine Oxidase