The Bacillus subtilis regulator protein SpoIIE shares functional and structural similarities with eukaryotic protein phosphatases 2C.
Dephosphorylation of SpoIIAA-P by SpoIIE is strictly dependent on the presence of the bivalent metal ions Mn2+ or Mg2+. Replacement by Ala of one of the four Asp residues, invariant in all representatives of protein phosphatase 2C, completely abolished the SpoIIE phosphatase activity in vitro, whilst replacement of the Asp residues by another acidic amino acid, Glu, had varying effects on the activities of the resulting mutated proteins. D610E and D795E exhibited some residual activity while D628E and D745E were without enzymatic activity. The results suggest that the functional model in which metal-associated water molecules are involved in the dephosphorylation reaction catalyzed by human protein phosphatase 2C alpha can also be applied to the bacterial protein phosphatase 2C-like protein.
Schroeter
R
R
Institut für Biologie, Humboldt Universität Berlin, Germany.
Schlisio
S
S
Lucet
I
I
Yudkin
M
M
Borriss
R
R
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics; D002413:Cations, Divalent / Q000494:pharmacology; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005057:Eukaryotic Cells; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008956:Models, Chemical; D016297:Mutagenesis, Site-Directed; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D011387:Prokaryotic Cells; D011994:Recombinant Proteins / Q000378:metabolism; D012808:Sigma Factor; D014157:Transcription Factors / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002413:Cations, Divalent; D011994:Recombinant Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; D008345:Manganese; D010749:Phosphoprotein Phosphatases; D008274:Magnesium