title: Differentiation-dependent switch in protein kinase C isoenzyme activation by FcgammaRI, the human high-affinity receptor for immunoglobulin G.
abstract: Aggregation of receptors for the constant region (Fc) of immunoglobulin G on myeloid cells results in endocytosis or phagocytosis and cellular activation. Previous work has shown, using the cell line U937, that the high-affinity immunoglobulin G receptor, FcgammaRI, activates alternate intracellular signalling pathways depending on the cell differentiation state, which results in a marked change in the nature of calcium transients within the cell. Here, we show that protein kinase C (PKC) is activated in both interferon-gamma (IFN-gamma) -primed and dibutyryl cyclic AMP (dbcAMP) -differentiated cells but that the nature of the particular isoenzymes recruited differs. Thus, in IFN-gamma-primed U937 cells, FcgammaRI aggregation results in an increase of PKC activity which is essentially calcium independent resulting from the translocation to the membrane of the novel PKCs, delta and epsilon, together with the atypical PKC zeta. However, in cells differentiated to a more macrophage phenotype, all PKC enzyme activity after receptor aggregation is calcium dependent. Consistent with this finding, the isoenzymes translocated to the nuclear-free membrane fraction are the conventional PKCs alpha, beta and gamma; results consistent with our previous finding that FcgammaRI couples to phospholipase C in such dbcAMP-differentiated cells. Thus, the nature of PKC isoenzyme activated following FcgammaRI aggregation is defined by differentiation. The calcium dependence of the PKC isoenzyme is consistent with the duration of calcium transients previously reported in the two differentiation states.
authors.affiliation: Department of Medicine and Therapeutics and Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Melendez
authors.forename: M M
authors.initials: MM
authors.lastname: Harnett
authors.forename: J M
authors.initials: JM
authors.lastname: Allen
mesh_terms: D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002462:Cell Membrane / Q000201:enzymology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D007527:Isoenzymes / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D017452:Receptors, IgG / Q000276:immunology*; D014178:Translocation, Genetic; D020298:U937 Cells / Q000201:enzymology / Q000276:immunology
chemical_list: D007527:Isoenzymes; D017452:Receptors, IgG; D003994:Bucladesine; D007371:Interferon-gamma; D011493:Protein Kinase C; D002118:Calcium