title: Biochemical and immunological changes on oral glutamate feeding in male albino rats.
abstract: High altitude stress leads to lipid peroxidation and free radical formation which results in cell membrane damage in organs and tissues, and associated mountain diseases. This paper discusses the changes in biochemical parameters and antibody response on feeding glutamate to male albino Sprague Dawley rats under hypoxic stress. Exposure of rats to simulated hypoxia at 7576 m, for 6 h daily for 5 consecutive days, in an animal decompression chamber at 32 +/- 2 degrees C resulted in an increase in plasma malondialdehyde level with a concomitant decrease in blood glutathione (reduced) level. Supplementation of glutamate orally at an optimal dose (27 mg/kg body weight) in male albino rats under hypoxia enhanced glutathione level and decreased malondialdehyde concentration significantly. Glutamate feeding improved total plasma protein and glucose levels under hypoxia. The activities of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) and the urea level remained elevated on glutamate supplementation under hypoxia. Glutamate supplementation increased the humoral response against sheep red blood cells (antibody titre). These results indicate a possible utility of glutamate in the amelioration of hypoxia-induced oxidative stress.
authors.affiliation: Department of Experimental Biology and Reproductive Physiology, Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi, India.
authors.forename: D
authors.initials: D
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Bansal
authors.forename: P
authors.initials: P
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Sairam
authors.forename: S K
authors.initials: SK
authors.lastname: Sharma
authors.forename: S S
authors.initials: SS
authors.lastname: Mongia
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.forename: W
authors.initials: W
authors.lastname: Selvamurthy
mesh_terms: D000284:Administration, Oral; D000410:Alanine Transaminase / Q000378:metabolism; D000417:Albinism; D000531:Altitude; D000532:Altitude Sickness / Q000517:prevention & control; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001786:Blood Glucose; D005609:Free Radicals; D018698:Glutamic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D005978:Glutathione / Q000097:blood / Q000187:drug effects; D000860:Hypoxia; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D001786:Blood Glucose; D005609:Free Radicals; D018698:Glutamic Acid; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione