The human superior cervical ganglion: neuropeptides and peptide receptors.
Noradrenaline (NA)- and neuropeptide Y (NPY)-containing cell bodies were found to occur in high numbers (>75% of all cells were positive) in the human superior cervical ganglion and distributed homogeneously throughout the ganglion and showed colocalisation. A few cell bodies were VIP-immunoreactive (-ir) (less than 5%) but none of them showed NOS-, CGRP- or SP-ir. Receptor mRNA expression was studied with RT-PCR. Total RNA from the superior cervical ganglion was successfully extracted. By using appropriate sense and antisense oligonucleotides designed from the published human sequences, we could show the presence of mRNA for the human NPY Y1, NPY Y2 and VPAC1 receptors but not CGRP1 receptor mRNA.
Tajti
J
J
Department of Internal Medicine, University Hospital of Lund, Sweden.
Möller
S
S
Uddman
R
R
Bodi
I
I
Edvinsson
L
L
D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009479:Neuropeptides / Q000032:analysis* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000235:genetics; D018013:Receptors, Neuropeptide / Q000032:analysis* / Q000235:genetics*; D017476:Receptors, Neuropeptide Y / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017783:Superior Cervical Ganglion / Q000378:metabolism*; D014158:Transcription, Genetic; D014660:Vasoactive Intestinal Peptide / Q000032:analysis / Q000235:genetics
D009479:Neuropeptides; D012333:RNA, Messenger; D018015:Receptors, Calcitonin Gene-Related Peptide; D018013:Receptors, Neuropeptide; D017476:Receptors, Neuropeptide Y; D014660:Vasoactive Intestinal Peptide