R.SspD5I is a neoschizomer of HphI producing blunt end DNA fragments.
The strain Staphylococcus species D5 produces a restriction enzyme. It is the neoschizomer of HphI endonuclease, which cleaves DNA at a distance of eight nucleotides from the recognition sequences producing blunt end DNA fragments: 5'-GGTGA8N /-3' and 3'-CCACT8N /-5'.
Zheleznaya
L
L
Institute of Theoretical and Applied Biophysics, RAS, Pushchino, Russia.
Shiryaev
S
S
Zheleznyakova
E
E
Matvienko
N
N
Matvienko
N
N
D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D013210:Staphylococcus / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024412:GGTGA-specific type II deoxyribonucleases