title: Endogenous serum levels of thrombopoietic cytokines in healthy whole-blood and platelet donors: implications for plateletpheresis.
abstract: Serum concentrations of the thrombopoiesis-enhancing cytokines thrombopoietin (TPO), erythropoietin (EPO), interleukin (IL)-6 and IL-11 were determined in 119 healthy whole-blood (WBD) and 101 platelet donors (PD) prior to donation. The 90% TPO reference interval in WBD of 64-867 pg/ml (median 163, 100% range 45-7572) was significantly higher than in PD of 56-524 (median 122, range 44-801, P = 0.004), whereas their platelet counts were lower (P < 0.001). EPO levels were not different (WBD 7.7 +/- 3.8, PD 8.0 +/- 4.9 IU/l), IL-6 and IL-11 were below the detection limit in >/=90% of cases (IL-6 < 3.2 pg/ml, IL-11 < 31.2 pg/ml). None of the cytokines correlated with platelet counts, other blood parameters, or in the PD group with the frequency of platelet donations within the last 6 months. We conclude that plateletpheresis does not lead to a lasting increase of thrombopoietic cytokines and provide reference data for potential platelet mobilization strategies with recombinant growth factors.
authors.affiliation: Haematology/Oncology, Carl-Neuberg-Strasse 1, D-30623, Hannover, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Tacke
authors.forename: P
authors.initials: P
authors.lastname: Schöffski
authors.forename: C
authors.initials: C
authors.lastname: Trautwein
authors.forename: M U
authors.initials: MU
authors.lastname: Martin
authors.forename: W
authors.initials: W
authors.lastname: Stangel
authors.forename: E
authors.initials: E
authors.lastname: Seifried
authors.forename: M P
authors.initials: MP
authors.lastname: Manns
authors.forename: A
authors.initials: A
authors.lastname: Ganser
authors.forename: D
authors.initials: D
authors.lastname: Petersen
mesh_terms: D000328:Adult; D000368:Aged; D001782:Blood Donors; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D017370:Interleukin-11 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D010983:Plateletpheresis; D013926:Thrombopoietin / Q000097:blood*
chemical_list: D016207:Cytokines; D017370:Interleukin-11; D015850:Interleukin-6; D013926:Thrombopoietin