Orphan receptor Arp-1 binds to the nucleotide sequence located between TATA box and transcriptional initiation site of the human angiotensinogen gene and reduces estrogen induced promoter activity.
Human angiotensinogen gene contains a C/A polymorphism at 20 bases upstream from the transcriptional initiation site. This sequence binds to the estrogen receptor when nucleoside A is present at this site and reporter constructs containing human angiotensinogen gene promoter with nucleoside A at -20 are transactivated on co-transfection of estrogen receptor in HepG2 cells followed by estrogen treatment. We show here that orphan receptor, Arp-1, which belongs to the COUP family of transcription factors also binds to this sequence. Co-transfection of Arp-1 reduces estrogen induced promoter activity of reporter constructs containing human angiotensinogen gene promoter. On the other hand co-transfection of Arp-1 does not have a significant effect on estrogen induced promoter activity of reporter constructs containing rat angiotensinogen gene promoter. Our data suggests that human and rat angiotensinogen genes are regulated in a different manner by estrogens.
Narayanan
C S
CS
Department of Pathology, New York Medical College, Valhalla 10595, USA.
Cui
Y
Y
Zhao
Y Y
YY
Zhou
J
J
Kumar
A
A
D000808:Angiotensinogen / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D006528:Carcinoma, Hepatocellular; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D017930:Genes, Reporter; D006801:Humans; D008113:Liver Neoplasms; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000187:drug effects; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism; D011987:Receptors, Steroid; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D000808:Angiotensinogen; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase; D004268:DNA-Binding Proteins; D004967:Estrogens; D011960:Receptors, Estrogen; D011987:Receptors, Steroid; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C497588:Nr2f2 protein, rat; C095569:NR2F2 protein, human