Co-transcription of Rhizobium meliloti lysyl-tRNA synthetase and glutamyl-tRNA synthetase genes.
An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypeptide of the expected mass (62 kDa), thus confirming that it encodes R. meliloti lysyl-tRNA synthetase. Reverse transcription/polymerase chain reaction was used to demonstrate that this lysS gene is co-transcribed with gltX in R. meliloti. This is the first reported case of two immediately adjacent and co-transcribed genes encoding aminoacyl-tRNA synthetases.
Pelchat
M
M
Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Sainte-Foy, Que., Canada.
Gagnon
Y
Y
Laberge
S
S
Lapointe
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli; D015870:Gene Expression; D005816:Genetic Complementation Test; D005975:Glutamate-tRNA Ligase / Q000235:genetics*; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D005975:Glutamate-tRNA Ligase; D008250:Lysine-tRNA Ligase