A sandwiched-culture technique for evaluation of heterologous protein production in a filamentous fungus.
Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ABH1 hydrophobin of Agaricus bisporus in A. niger, we showed that low production levels of the heterologous protein are probably due to extracellular proteolytic degradation of the protein.
Asgeirsdóttir
S A
SA
Molecular Plant Biology Laboratory, Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands. s.asgeirsdottir@farm.rug.nl
Scholtmeijer
K
K
Wessels
J G
JG
D000364:Agaricus / Q000235:genetics; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000235:genetics* / Q000378:metabolism*; D001709:Biotechnology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D009172:Mycology / Q000379:methods; D015202:Protein Engineering; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*
C100774:ABH1 protein, Agaricus bisporus; D005656:Fungal Proteins; D011994:Recombinant Proteins