New tetrazolium method for the histochemical demonstration of gamma-glutamyl transpeptidase.
A new tetrazolium method for the histochemical demonstration of gamma-glutamyl transpeptidase is proposed. The method is based on a newly synthesized substrate-gamma-L-glutamic acid-1-hydroxy-4-naphthylamide, which upon the enzyme hydrolysis liberates 1,4-aminonaphthol--a powerful reducing agent that reduces tetrazolium salts quickly and quantitatively to deeply colored, water-insoluble formazans, precipitating on the sites of the enzyme activity and marking them accurately. The redox reaction is quick enough and does not need any auxiliary electron-acceptor. The method is very fast and convenient for the histochemical visualization of the enzyme.
Dikov
A
A
Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia.
Dimitrova
M
M
Krieg
R
R
Halbhuber
K J
KJ
D000818:Animals; D007202:Indicators and Reagents; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D005723:gamma-Glutamyltransferase