Charge derivatization of peptides for analysis by mass spectrometry.
The analysis of peptide derivatives by fast atom bombardment, liquid secondary-ionization mass spectrometry, plasma desorption, electrospray ionization, and matrix-assisted laser desorption/ionization is reviewed. The fragmentation patterns of peptides and of charge-derivatized peptides are compared, and the proposed fragment ion structures are summarized. A variety of derivatization approaches and the distinguishing features of mass spectra produced from these derivatives are described. The most promising derivatization approaches are evaluated, and the strengths and limitations of these approaches are discussed.
Roth
K D
KD
Department of Chemistry, Michigan State University, East Lansing 48824, USA.
Huang
Z H
ZH
Sadagopan
N
N
Watson
J T
JT
D000595:Amino Acid Sequence; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000379:methods*; D009943:Organophosphorus Compounds; D010455:Peptides / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods
D007202:Indicators and Reagents; D009943:Organophosphorus Compounds; D010455:Peptides; D000644:Quaternary Ammonium Compounds