alpha4 integrin-dependent eotaxin induction of bronchial hyperresponsiveness and eosinophil migration in interleukin-5 transgenic mice.
We investigated the roles of eosinophil infiltration and activation induced by the eosinophil-selective chemokine eotaxin, and of the expression of eosinophil alpha4 and beta2 integrins in causing bronchial hyperresponsiveness (BHR) in interleukin (IL)-5 CBA/Ca transgenic mice. These mice did not show BHR, despite the presence of some eosinophils in the lungs. Intratracheal mouse recombinant eotaxin (3 micrograms) did not induce BHR in wild-type mice. In IL-5 transgenic mice, eotaxin (3 and 5 micrograms) increased responsiveness at 24 h and increased eosinophils in bronchoalveolar lavage (BAL) fluid by 9.4- and 14-fold by 24 h, respectively, together with augmentation of eosinophil peroxidase activity and eosinophil infiltration in the airway submucosa. Using flow cytometry, the expression of alpha4, CD11b, and CD18 was upregulated in BAL, but not in blood, eosinophils. A rat anti-alpha4 antibody inhibited eotaxin-induced BHR and eosinophil migration and activation, but an anti-CD11b antibody had no significant effects on BHR. A combination of both antibodies was more effective. IL-5 and eotaxin synergize in the induction of BHR and airway eosinophilia, effects that are dependent on the induction of eosinophil alpha4 integrin. Expression of BHR depends on the recruitment and activation of eosinophils.
Hisada
T
T
National Heart and Lung Institute at Imperial College School of Medicine, London, United Kingdom.
Hellewell
P G
PG
Teixeira
M M
MM
Malm
M G
MG
Salmon
M
M
Huang
T J
TJ
Chung
K F
KF
D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002465:Cell Movement; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines / Q000494:pharmacology*; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D005434:Flow Cytometry; D039441:Integrin alpha4; D015848:Interleukin-5 / Q000235:genetics*; D051379:Mice; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D010544:Peroxidases / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D015703:Antigens, CD; C515457:Ccl11 protein, mouse; C515458:Ccl11 protein, rat; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D015848:Interleukin-5; D011994:Recombinant Proteins; D039441:Integrin alpha4; D047209:Eosinophil Peroxidase; D010544:Peroxidases