Effects of ALCAR on the fast axoplasmic transport in cultured sensory neurons of streptozotocin-induced diabetic rats.
The effects of acetyl-L-carnitine (ALCAR) on fast axoplasmic transport were studied in cultured dorsal root ganglion (DRG) neurons of diabetic rats. Three-month-old male rats were used 7 days after streptozotocin injection. Neurons obtained from ganglia were cultured with a high concentration of glucose. The amount and the mean velocity of retrogradely transported particles, reduced in the diabetic animal, were transiently recovered by 1 mM ALCAR. The number of particles moving at 0.8-1.2 microm/s, considered to be lysosomes, increased in the velocity distribution. ALCAR did not modify the amount and mean velocity of anterograde particles which were unaffected by diabetes, or of bidirectional particles in neurons of control rats. This study suggests that diabetic neuropathy may be relieved by ALCAR via recovering retrograde axoplasmic transport.
Kano
M
M
Department of Physiology, Yokohama City University School of Medicine, Japan.
Kawakami
T
T
Hori
H
H
Hashimoto
Y
Y
Tao
Y
Y
Ishikawa
Y
Y
Takenaka
T
T
D000108:Acetylcarnitine / Q000494:pharmacology*; D000344:Afferent Pathways / Q000503:physiopathology; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004525:Efferent Pathways / Q000503:physiopathology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000503:physiopathology*; D007700:Kinetics; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014741:Video Recording
D001786:Blood Glucose; D000108:Acetylcarnitine