title: Isolation of the LEMMI9 gene and promoter analysis during a compatible plant-nematode interaction.
abstract: Plant-endoparasitic root-knot nematodes feed on specialized giant cells that they induce in the vascular cylinder of susceptible plants. Although it has been established that a number of plant genes change their expression pattern during giant cell differentiation, virtually no data are available about the mechanisms involved in that change. One possibility is differential promoter recognition by the transcription factor(s) responsible for the expression of specific genes. We have isolated and characterized a genomic clone from tomato containing the promoter region of LEMMI9, one of the few plant genes that have been reported to be highly expressed in galls (predominantly in giant cells). The analysis of transgenic potato plants carrying a LEMMI9 promoter-beta glucuronidase (GUS) fusion has demonstrated that the tomato promoter was activated in Meloidogyne incognita-induced galls in a heterologous system. We have located putative regulatory sequences in the promoter and have found that nuclear proteins from the galls formed specific DNA-protein complexes with the proximal region of the LEMMI9 promoter. The nuclear protein-binding sequence mapped to a region of 111 bp immediately upstream from the TATA box. This region contains a 12-bp repeat possibly involved in the formation of DNA-protein complexes, which might be related to the LEMMI9 transcriptional activation in the giant cells.
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Escobar
authors.forename: J
authors.initials: J
authors.lastname: De Meutter
authors.forename: F A
authors.initials: FA
authors.lastname: Aristizábal
authors.forename: S
authors.initials: S
authors.lastname: Sanz-Alférez
authors.forename: F F
authors.initials: FF
authors.lastname: del Campo
authors.forename: N
authors.initials: N
authors.lastname: Barthels
authors.forename: W
authors.initials: W
authors.lastname: Van der Eycken
authors.forename: J
authors.initials: J
authors.lastname: Seurinck
authors.forename: M
authors.initials: M
authors.lastname: van Montagu
authors.forename: G
authors.initials: G
authors.lastname: Gheysen
authors.forename: C
authors.initials: C
authors.lastname: Fenoll
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*; D008969:Molecular Sequence Data; D009348:Nematoda / Q000472:pathogenicity*; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011198:Solanum tuberosum / Q000235:genetics
chemical_list: D018744:DNA, Plant; D009687:Nuclear Proteins; D010940:Plant Proteins