Colocalization of the herpes simplex virus 1 UL4 protein with infected cell protein 22 in small, dense nuclear structures formed prior to onset of DNA synthesis.
Herpes simplex virus 1 infected cell protein 22 (ICP22) localizes in small, dense nuclear bodies of primate cells early in infection and in the more diffuse replicative complexes after the onset of DNA synthesis. UL4, a gamma2 protein, localizes in cytoplasm and in the small nuclear structures containing ICP22 but not in replicative complexes. In rabbit skin cells, both ICP22 and UL4 localize in the dense nuclear bodies late in infection. The results suggest that the small nuclear structures perform a function involving both proteins late in infection.
Jahedi
S
S
The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637, USA.
Markovitz
N S
NS
Filatov
F
F
Roizman
B
B
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D004279:DNA, Viral / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D018259:Herpesvirus 1, Human / Q000737:chemistry*; D017874:Immediate-Early Proteins / Q000032:analysis*; D016366:Open Reading Frames; D011817:Rabbits; D014764:Viral Proteins / Q000032:analysis*; D054334:Viral Regulatory and Accessory Proteins
D004279:DNA, Viral; C057294:ICP22 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C078424:EUS1 protein, Equine herpesvirus 1