Spermatocytic seminoma as compared to classical seminoma: an immunohistochemical and DNA flow cytometric study.
Based on immunohistochemistry (IHC) and DNA ploidy, different paths of carcinogenesis have been suggested for spermatocytic seminoma (SS) and classical seminoma (CS). The present study extends current knowledge on the above parameters.


METHODS
Seventeen SSs and twenty-two CSs were assessed by IHC for placental-like alkaline phosphatase (PLAP), c-kit, cytokeratin and adhesion carbohydrate molecyles. All SSs and 11 CSs were also analysed for DNA ploidy.


RESULTS
All CSs, but none of the SSs, were positive for PLAP. C-kit positivity was found in 7 of 17 SSs and in all CSs. The other IHC parameters were similarly distributed among the evaluated SSs and CSs. Fourteen SSs were diploid or polyploid, and three were aneuploid. All CSs were aneuploid.


CONCLUSIONS
The new observation of c-kit positivity in about 40% of SSs suggests that at least some of the SSs originate from primordial cells. The predominantly diploid or polyploid DNA pattern indicates that SSs follow a pathogenetic pathway which is most probably different from that of CSs.
Kraggerud
S M
SM
Department of Cell Biology, The Norwegian Radium Hospital, Oslo.
Berner
A
A
Bryne
M
M
Pettersen
E O
EO
Fossa
S D
SD
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D000782:Aneuploidy; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D004171:Diploidy; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011123:Polyploidy; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D018239:Seminoma / Q000235:genetics* / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000235:genetics* / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D004273:DNA, Neoplasm; D007527:Isoenzymes; C055791:germ-cell AP isoenzyme; D007633:Keratins; D019009:Proto-Oncogene Proteins c-kit; D000469:Alkaline Phosphatase