title: Study of interleukin-6 and tumor necrosis factor-alpha levels in maternal serum and amniotic fluid of patients with premature rupture of membranes.
abstract: To study the change and clinical significance of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) levels in the maternal serum and amniotic fluid of pregnant women with chorioamnionitis and with premature rupture of membranes. Twenty-six normal-term pregnant women formed the control group, and forty-six pregnant women with premature rupture of membranes were enrolled for the study. Maternal serum and amniotic fluid IL-6 and TNF-alpha levels were measured using a sensitive radioimmunoassay and enzyme-linked immunosorbent assay (ELISA); chorioamnionitis was diagnosed by fetal membrane pathology. The maternal serum IL-6 levels and amniotic fluid IL-6 and TNF-alpha levels were higher than those of the control (P < 0.01). There was a significant relationship between maternal serum IL-6 and maternal serum and amniotic fluid IL-6 and TNF-alpha with the time of the premature rupture of membranes, i.e. the longer the time, the higher the maternal serum and amniotic fluid IL-6 and TNF-alpha. There were 12 patients with chorioamnionitis in premature rupture of membranes and their maternal serum and amniotic fluid IL-6 and TNF-alpha levels were higher than that of non-chorioamnionitis patients (P < 0.01-0.05). IL-6 and TNF-alpha levels in maternal and amniotic fluids are a valuable index in identification of the chorioamnionitis in patients with premature rupture of membranes.
authors.affiliation: Department of Obstetrics and Gynecology, Second Teaching Hospital, Bethune University of Medical Sciences, Changchun, P. R. of China. zhangwy@public.cc.jl.cn
authors.forename: Z
authors.initials: Z
authors.lastname: Weiyuan
authors.forename: W
authors.initials: W
authors.lastname: Li
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D002821:Chorioamnionitis / Q000150:complications / Q000175:diagnosis; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000097:blood / Q000150:complications / Q000378:metabolism*; D005865:Gestational Age; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis* / Q000097:blood; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha