A new solid-phase synthesis of oligonucleotides 3'-conjugated with peptides.
A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the first nucleoside via the base has been developed. A 17-mer designed for antisense experiments against HIV-1, linking at the 3'-terminus the tripeptide Gly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry.
De Napoli
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Dipartimento di Chimica Organica e Biologica, Università degli Studi di Napoli Federico II, Italy.
Messere
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Montesarchio
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Piccialli
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Benedetti
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Bucci
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Rossi
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D002621:Chemistry / Q000379:methods*; D015497:HIV-1 / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010452:Peptide Biosynthesis; D013696:Temperature
D009841:Oligonucleotides