title: Inhibition of peroxidase, trypsin, and alpha-chymotrypsin by platinum (II) and platinum (IV) complexes.
abstract: Effects of various complexes of platinum (II) and platinum (IV) on activities of trypsin, alpha-chymotrypsin, and peroxidase were compared. The platinum (II) complexes were found to inhibit these enzymes, though with variable efficiency. The platinum (IV) complexes at concentrations < or = 0.2 mM efficiently inhibited peroxidase but had no effect on the proteases. An enzymatic assay was developed to measure the most effective peroxidase inhibitor (cisplatin) at concentrations of 5-50 microM in the presence of fivefold excess of its isomer (transplatin).
authors.affiliation: School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Muginova
authors.forename: E V
authors.initials: EV
authors.lastname: Vil'ms
authors.forename: T N
authors.initials: TN
authors.lastname: Shekhovtsova
authors.forename: V B
authors.initials: VB
authors.lastname: Ivanov
mesh_terms: D002384:Catalysis; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors*; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D009944:Organoplatinum Compounds; D014361:Trypsin Inhibitors; D006735:Horseradish Peroxidase; D002918:Chymotrypsin