Regulation of the expression of the Kluyveromyces lactis PDC1 gene: carbon source-responsive elements and autoregulation.
The yeast Kluyveromyces lactis has a single structural gene coding for pyruvate decarboxylase (KIPDC1). In order to study the regulation of the expression of KIPDC1, we have sequenced (EMBL Accession No. Y15435) its promoter and have fused the promoter to the reporter gene lacZ from E. coli. Transcription analysis in a Klpdc1 delta strain showed that KIPDC1 expression is subject to autoregulation. The PDC1 gene from Saccharomyces cerevisiae was able to complement the Rag- phenotype of the Klpdc1 delta mutant strain and it could also repress transcription of the KIPDC1-lacZ fusion on glucose. A deletion analysis of the promoter region was performed to study carbon source-dependent regulation and revealed that at least two cis-acting regions are necessary for full induction of gene expression on glucose. Other cis-elements mediate repression on ethanol.
Destruelle
M
M
Pasteur Institute, Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome La Sapienza, Rome, Italy.
Menghini
R
R
Frontali
L
L
Bianchi
M M
MM
D015152:Blotting, Northern; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011767:Pyruvate Decarboxylase / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic
D011993:Recombinant Fusion Proteins; D011767:Pyruvate Decarboxylase; D005947:Glucose