Procoagulant activity and cytokine expression in whole blood cultures from patients with atherosclerosis supplemented with omega-3 fatty acids.
Omega-3 fatty acids (n-3 FA) may reduce atherogenesis and thrombosis. We investigated the effects of n-3 FAs on procoagulant activity and cytokine expression in whole blood cultures from patients with atherosclerosis. Eleven of the 23 included patients had received 5.1 g n-3 FA daily for 6 months (group I) whereas 12 patients had been on placebo (group II). All patients were then given 5. g n-3 FA daily for another 4 weeks. At baseline significantly lower levels of LPS-induced prothrombin fragment1+2 were found in group I (p = 0.010), this difference being eliminated after 4 weeks. Il-6 and TNFalpha were significantly higher at baseline in group I and the differences in changes from baseline between the groups were statistically highly significant with increasing values in group II(Il-6 p = 0.001, TNF alpha p = 0.002). The present results indicate a reduction in pro-thrombotic potential in patients receiving highly concentrated n-3 FA, whereas some proinflammatory responses might be adverse.
Seljeflot
I
I
Dept of Clinical Chemistry, Ullevål University Hospital, Oslo, Norway. ingebjorg.seljeflot@ulleval.no
Johansen
O
O
Arnesen
H
H
Eggesbø
J B
JB
Westvik
A B
AB
Kierulf
P
P
D000328:Adult; D000368:Aged; D001161:Arteriosclerosis / Q000097:blood* / Q000188:drug therapy*; D001772:Blood Cell Count; D016207:Cytokines / Q000097:blood*; D004311:Double-Blind Method; D005169:Factor VIII / Q000187:drug effects; D005227:Fatty Acids / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008074:Lipoproteins / Q000097:blood; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D014810:Vitamin E / Q000097:blood
D016207:Cytokines; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D008074:Lipoproteins; D010743:Phospholipids; D014810:Vitamin E; D005169:Factor VIII