Differential expression of two skeletal muscle beta-tropomyosin mRNAs during Xenopus laevis development.
A cDNA clone for a Xenopus laevis skeletal muscle beta-tropomyosin (beta-TMad) isoform was isolated from an adult skeletal muscle cDNA library. Sequence analysis revealed that this clone corresponded to a second beta-tropomyosin mRNA distinct from the one that was previously characterized (beta-TMemb). The two skeletal beta-TM mRNAs originate from distinct genes and are differentially expressed during development. Beta-TMemb mRNA is expressed only in the somites of the early embryo while beta-TMad mRNA is expressed in pre-metamorphic tadpoles and adult skeletal muscles. We have isolated the promoter region of the beta-TMemb gene and shown that a DNA construct containing 2.9 kb of promoter region is properly expressed after injection in the embryo.
Gaillard
C
C
ISREC, Epalinges/Lausanne, Switzerland.
Lerivray
H
H
Thézé
N
N
Cooper
B
B
Lepetit
D
D
Mohun
T
T
Thiébaud
P
P
D000818:Animals; D001483:Base Sequence; D019070:Cell Lineage; D018507:Gene Expression Regulation, Developmental; D008957:Models, Genetic; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors; D014018:Tissue Distribution; D014335:Tropomyosin / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*
D014335:Tropomyosin