title: Mutant alleles of the Drosophila trithorax gene produce common and unusual homeotic and other developmental phenotypes.
abstract: trithorax (trx) encodes chromosome-binding proteins required throughout embryogenesis and imaginal development for tissue- and cell-specific levels of transcription of many genes including homeotic genes of the ANT-C and BX-C. trx encodes two protein isoforms that contain conserved motifs including a C-terminal SET domain, central PHD fingers, an N-terminal DNA-binding homology, and two short motifs also found in the TRX human homologue, ALL1. As a first step to characterizing specific developmental functions of TRX, I examined phenotypes of 420 combinations of 21 trx alleles. Among these are 8 hypomorphic alleles that are sufficient for embryogenesis but provide different levels of trx function at homeotic genes in imaginal cells. One allele alters the N terminus of TRX, which severely impairs larval and imaginal growth. Hypomorphic alleles that alter different regions of TRX equivalently reduce function at affected genes, suggesting TRX interacts with common factors at different target genes. All hypomorphic alleles examined complement one another, suggesting cooperative TRX function at target genes. Comparative effects of hypomorphic genotypes support previous findings that TRX has tissue-specific interactions with other factors at each target gene. Some hypomorphic genotypes also produce phenotypes that suggest TRX may be a component of signal transduction pathways that provide tissue- and cell-specific levels of target gene transcription.
authors.affiliation: Department of Zoology, Southern Illinois University, Carbondale, Illinois 62901-6501, USA. tbreen@zoology.siu.edu
authors.forename: T R
authors.initials: TR
authors.lastname: Breen
mesh_terms: D000483:Alleles; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics*; D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D005804:Genes, Lethal; D005838:Genotype; D008957:Models, Genetic; D016296:Mutagenesis; D019683:Penetrance; D010641:Phenotype; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C063460:Trl protein, Drosophila