A bioseparation apparatus with high-pressure fluid injection and fluid sampling.
A novel apparatus in which fluids may be injected and sampled at high pressure is described. Bioseparation applications of the apparatus were demonstrated in three model systems: (1) lambdaDNA was eluted under pressure from an anion exchange column into a low-salt (0.25 M) buffer, thereby eliminating conventional time-consuming desalting procedures required for downstream analysis of the DNA; (2) RNA was separated under pressure from a RNA/DNA mixture, thereby enabling rapid differential preparation of nucleic acids; and (3) an antibody was purified from a protein mixture by affinity capture at one pressure and dissociation from the antigen binding partner at a second pressure, thereby enabling the immunoreactivities of both antibody and antigen to be preserved during the separation process.
Dreier
G H
GH
BMA Laboratories, Inc., BioSeq, Inc., 25 Olympia Avenue, Unit F, Woburn, Massachusetts 01801-6307, USA.
Tao
F
F
Hess
R A
RA
Cheung
C Y
CY
Sciaba
L E
LE
Green
D J
DJ
Laugharn
J A
JA
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D010582:Bacteriophage lambda / Q000737:chemistry; D001671:Biochemistry / Q000295:instrumentation*; D002021:Buffers; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004279:DNA, Viral / Q000302:isolation & purification; D006874:Hydrostatic Pressure; D012313:RNA / Q000302:isolation & purification
D000906:Antibodies; D002021:Buffers; D004279:DNA, Viral; D012313:RNA