title: Effect of vitamin E in gastric mucosal injury induced by ischaemia-reperfusion in nitric oxide-depleted rats.
abstract: BACKGROUND
  Neutrophil infiltration and lipid peroxide accumulation are involved in reperfusion-induced gastric mucosal injury in nitric oxide-depleted rats.
  
  
  OBJECTIVE
  To assess the effect of vitamin E on this injury.
  
  
  METHODS
  After ischaemia-reperfusion, the total area of erosions, lipid peroxide contents in gastric mucosa, and gastric neutrophil accumulation were compared between nitric oxide-depleted rats with deficient, normal, and increased vitamin E intake over 8 weeks. Thiobarbituric acid-reactive substances and tissue-associated myeloperoxidase activity were measured in gastric mucosa as indices of lipid peroxidation and neutrophil infiltration.
  
  
  RESULTS
  The total area of erosions was significantly increased in the vitamin E-deficient group compared with the sufficient-intake and vitamin-supplemented groups. Both thiobarbituric acid-reactive substances and myeloperoxidase activity also were significantly increased in the vitamin E-deficient group compared with others. The total area of erosions closely paralleled the increases in both thiobarbituric acid-reactive substances and myeloperoxidase activity.
  
  
  CONCLUSIONS
  These results indicate that the inhibition of lipid peroxidation and interference with neutrophil infiltration by vitamin E may be responsible for its cytoprotective effect in ischaemia-reperfusion.
authors.affiliation: First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan. ynaito@koto.kpu-m.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: K
authors.initials: K
authors.lastname: Matsuyama
authors.forename: N
authors.initials: N
authors.lastname: Yagi
authors.forename: K
authors.initials: K
authors.lastname: Kasai
authors.forename: N
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Masui
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Kondo
mesh_terms: D000818:Animals; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D020011:Protective Agents / Q000378:metabolism / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications / Q000503:physiopathology / Q000517:prevention & control*; D013272:Stomach Diseases / Q000209:etiology / Q000517:prevention & control*; D014810:Vitamin E / Q000378:metabolism / Q000627:therapeutic use*; D014811:Vitamin E Deficiency / Q000503:physiopathology
chemical_list: D020011:Protective Agents; D014810:Vitamin E; D009569:Nitric Oxide