Alterations of lymphocyte populations in lymph nodes but not in spleen during the latency period of adjuvant arthritis.
The aim of this study was to analyze potential imbalances in lymphocyte populations from regional lymph nodes (LN) and spleen occurring before the development of the outer inflammation of adjuvant arthritis (AA). Percentages and absolute numbers of CD5+, CD4+, CD8+, Ig+, I-A+, NKR-P1+ and TCRgammadelta+ cells were determined. No differences in percentages of gammadelta T or NK cells were found either in LN or spleen, thus ruling out an important role of these minor subpopulations in these early stages of AA. While no significant lymphocyte imbalances were observed in spleen, an increase in the percentage of B lymphocytes was found in regional LN. Moreover, a high proliferation of CD8+ cells was observed when measuring absolute numbers of LN lymphocytes, thus producing an imbalance in the CD4/CD8 ratio at very early stages of the inflammatory process. These findings suggest a role for CD8+ and B lymphocytes in the latency period of AA at the LN level. Our results indicate a primary role for lymph nodes in initiating the inflammation of AA, whereas cells from the spleen probably play a secondary role.
Rodríguez-Palmero
M
M
Department de Fisiologia-Divisió IV, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Pelegrí
C
C
Ferri
M J
MJ
Castell
M
M
Franch
A
A
Castellote
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D001169:Arthritis, Experimental / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D016692:Receptors, Antigen, T-Cell, gamma-delta