NK-1 receptor immunoreactivity in distinct morphological types of lamina I neurons of the primate spinal cord.
In cat and monkey, lamina I cells can be classified into three basic morphological types (fusiform, pyramidal, and multipolar), and recent intracellular labeling evidence in the cat indicates that fusiform and multipolar lamina I cells are two different types of nociceptive cells, whereas pyramidal cells are innocuous thermoreceptive-specific. Because earlier observations indicated that only nociceptive dorsal horn neurons respond to substance P (SP), we examined which morphological types of lamina I neurons express receptors for SP (NK-1r). We categorized NK-1r-immunoreactive (IR) lamina I neurons in serial horizontal sections from the cervical and lumbar enlargements of four monkeys. Consistent results were obtained by two independent teams of observers. Nearly all NK-1r-IR cells were fusiform (42%) or multipolar (43%), but only 6% were pyramidal (with 9% unclassified). We obtained similar findings in three monkeys in which we used double-labeling immunocytochemistry to identify NK-1r-IR and spinothalamic lamina I neurons retrogradely labeled with cholera toxin subunit b from the thalamus; most NK-1r-IR lamina I spinothalamic neurons were fusiform (48%) or multipolar (33%), and only 10% were pyramidal. In contrast, most (approximately 75%) pyramidal and some (approximately 25%) fusiform and multipolar lamina I spinothalamic neurons did not display NK-1r immunoreactivity. These data indicate that most fusiform and multipolar lamina I neurons in the monkey can express NK-1r, consistent with the idea that both types are nociceptive, whereas only a small proportion of lamina I pyramidal cells express this receptor, consistent with the previous finding that they are non-nociceptive. However, these findings also indicate that not all nociceptive lamina I neurons express receptors for SP.
Yu
X H
XH
Department of Pharmacology and Therapeutics, McGill University, Montréal, Quebec, H3G 1Y6 Canada.
Zhang
E T
ET
Craig
A D
AD
Shigemoto
R
R
Ribeiro-da-Silva
A
A
De Koninck
Y
Y
D000818:Animals; D001370:Axonal Transport; D002415:Cats; D002772:Cholera Toxin; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D008253:Macaca mulatta; D009474:Neurons / Q000145:classification* / Q000166:cytology* / Q000502:physiology; D017966:Pyramidal Cells / Q000166:cytology* / Q000502:physiology; D018040:Receptors, Neurokinin-1 / Q000032:analysis*; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology
D018040:Receptors, Neurokinin-1; D002772:Cholera Toxin