Immunohistochemical analysis, human papillomavirus DNA detection, hormonal manipulation, and exogenous gene expression of normal and dysplastic human cervical epithelium in severe combined immunodeficiency mice.
The cervical squamocolumnar junction of normal and dysplastic human xenografts was maintained in SCID-beige mice. Dysplastic tissue maintained a dysplastic morphology, irregular pattern of keratin expression, elevated levels of cellular proliferation, and human papillomavirus type 16 and/or type 18 DNA. Hyperplastic changes of normal xenografts occurred via high-dose estrogen exposure, and through recombinant adenovirus infection, the introduction and stable expression of an exogenous gene was accomplished.
Taylor
J A
JA
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California 92697, USA.
Tewari
K
K
Liao
S Y
SY
Hughes
C C
CC
Villarreal
L P
LP
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002455:Cell Division; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D027383:Papillomaviridae / Q000235:genetics*; D014183:Transplantation, Heterologous; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D001616:beta-Galactosidase / Q000235:genetics
D004279:DNA, Viral; D004958:Estradiol; D007633:Keratins; D001616:beta-Galactosidase