Isolation and characterization of the Saccharomyces cerevisiae XPT1 gene encoding xanthine phosphoribosyl transferase.
A new Saccharomyces cerevisiae gene, XPT1, was isolated as a multicopy suppressor of a hypoxanthine phosphoribosyl transferase (HPRT) defect. Disruption of XPT1 affects xanthine utilization in vivo and results in a severe reduction of xanthine phosphoribosyl transferase (XPRT) activity while HPRT is unaffected. We conclude that XPT1 encodes XPRT in yeast.
Guetsova
M L
ML
Institut de Biochimie et Génétique Cellulaires, CNRS UPR9026, F-33077 Bordeaux Cedex, France.
Crother
T R
TR
Taylor
M W
MW
Daignan-Fornier
B
B
D005800:Genes, Fungal; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D010430:Pentosyltransferases / Q000235:genetics*; D011687:Purines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013489:Suppression, Genetic; D019820:Xanthine / Q000378:metabolism*
D011687:Purines; D019820:Xanthine; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase