Increase of galanin mRNA in lumbar dorsal root ganglion neurons of adult rats after partial sciatic nerve ligation.
Partial sciatic nerve ligation (PSNL) is a widely used model for the study of neuropathic pain. However, there is little information on neuropeptide expression in primary sensory neurons after PSNL. We examined galanin (GAL) mRNA expression in L4 and L5 dorsal root ganglion (DRG) neurons of adult rats after PSNL. We found that 4 and 14 days after PSNL the percentages of GAL mRNA positive neurons were significantly increased in the ipsilateral DRG compared to the contralateral side. Using combined retrograde fluorescent dye tracing and in situ hybridization, we found that 47% of the injured neurons and 10% of the spared neurons were GAL mRNA positive. Since only 2-3% of neurons in the contralateral uninjured DRG were GAL mRNA positive, PSNL induced up-regulation of GAL mRNA in both injured and spared DRG neurons.
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Department of Physiology, Faculty of Medicine, Queen's University, Kingston, Ontario, Canada. maweiy@douglas.mcgill.ca
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D000818:Animals; D001370:Axonal Transport; D005456:Fluorescent Dyes; D019004:Galanin / Q000235:genetics*; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000502:physiology / Q000503:physiopathology; D005786:Gene Expression Regulation; D008297:Male; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology* / Q000503:physiopathology; D013266:Stilbamidines; D014158:Transcription, Genetic
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D013266:Stilbamidines; D019004:Galanin