Regulation of blood pressure during long-term ouabain infusion in Long-Evans rats.
We tested whether ouabain, an inhibitor of the sodium pump, can lead to chronic hypertension in Long-Evans rats using sensitive 24-h measurements of blood pressure. After a control week of vehicle isotonic saline infusion (14.4 mL/day), ouabain was infused intravenously at 30 microg/kg/day in intact (2K) and uninephrectomized (1K) Long-Evans rats for a total of 4 weeks. Although plasma ouabain concentration rose to 0.97 +/- 0.15 nmol/L with ouabain infusion, mean arterial pressure did not change in either 2K (delta = -0.6 +/- 1.3 mm Hg) or 1K (delta = -1.2 +/- 0.7 mm Hg) rats. These data suggest that Long-Evans rats are insensitive to the hypertensive effects of ouabain.
Wang
J
J
Institute of Physiology, University of Fribourg, Switzerland.
Tempini
A
A
Schnyder
B
B
Montani
J P
JP
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D007262:Infusions, Intravenous; D008297:Male; D010042:Ouabain / Q000097:blood / Q000494:pharmacology*; D011188:Potassium / Q000097:blood / Q000652:urine; D051381:Rats; D020318:Rats, Long-Evans; D012964:Sodium / Q000097:blood / Q000652:urine; D013997:Time Factors
D004791:Enzyme Inhibitors; D010042:Ouabain; D012964:Sodium; D011188:Potassium