ChT1, an Ig superfamily molecule required for T cell differentiation.
The thymus is colonized by circulating progenitor cells that differentiate into mature T cells under the influence of the thymic microenvironment. We report here the cloning and function of the avian thymocyte Ag ChT1, a member of the Ig superfamily with one V-like and one C2-like domain. ChT1-positive embryonic bone marrow cells coexpressing c-kit give rise to mature T cells upon intrathymic cell transfer. ChT1-specific Ab inhibits T cell differentiation in embryonic thymic organ cultures and in thymocyte precursor cocultures on stromal cells. Thus, we provide clear evidence that ChT1 is a novel Ag on early T cell progenitors that plays an important role in the early stages of T cell development.
Katevuo
K
K
Turku Immunology Center, Department of Medical Microbiology, University of Turku, Turku, Finland. kaisa.katevuo@utu.fi
Imhof
B A
BA
Boyd
R
R
Chidgey
A
A
Bean
A
A
Dunon
D
D
Göbel
T W
TW
Vainio
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D018956:CD5 Antigens / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D008221:Lymphoid Tissue / Q000196:embryology / Q000276:immunology; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D019009:Proto-Oncogene Proteins c-kit / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000601:surgery; D014018:Tissue Distribution
D000954:Antigens, Surface; D018956:CD5 Antigens; D018076:DNA, Complementary; D007136:Immunoglobulins; D008565:Membrane Proteins; D019009:Proto-Oncogene Proteins c-kit