title: Detection of circulating antigens and antibodies in Toxocara canis infection among children in Chengdu, China.
abstract: In order to determine the seroprevalence of Toxocara spp. infection in children from Chengdu, we performed an enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA (S-ELISA) with excretory-secretory antigens isolated from second-stage larvae of Toxocara canis (TES-Ag ELISA). The seroprevalences of T. canis antibodies in the children from rural areas, urban districts, and urban districts with recent Ascaris lumbricoides infection were 17.7% (59/333), 2.1% (4/186), and 2.6% (1/38), respectively. Among 63 suspected patients with symptoms of T. canis infection, 31 had positive antibodies. The inhibition assay showed an apparent inhibiting capacity of TES-Ag for the antibody against T. canis larvae. The result of S-ELISA demonstrated that circulating antigens of T. canis larvae could be detected in part of the serum with positive antibodies and that the detection rate for circulating antigens in the sera could be improved by polyethylene glycol-acid treatment. This is the first epidemiological study to confirm the existence of T. canis infection and Toxocara-larvae migrans in Chengdu by the combination of TES-Ag ELISA and S-ELISA.
authors.affiliation: Department of Parasitology, West China University of Medical Sciences, Chengdu Sichuan, The People's Republic of China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Luo
authors.forename: G X
authors.initials: GX
authors.lastname: Wang
authors.forename: C I
authors.initials: CI
authors.lastname: Yang
authors.forename: C H
authors.initials: CH
authors.lastname: Luo
authors.forename: S W
authors.initials: SW
authors.lastname: Cheng
authors.forename: L
authors.initials: L
authors.lastname: Liao
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007816:Larva Migrans, Visceral / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D012424:Rural Population; D016036:Seroepidemiologic Studies; D017166:Toxocara canis / Q000276:immunology* / Q000302:isolation & purification*; D014120:Toxocariasis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D014505:Urban Population
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth