Temperature and neuromuscular development in embryos of the trout (Salmo trutta L.).
Myogenesis and neural development were examined in the myotomes of trout (Salmo trutta L.) embryos reared at 2, 6 and 10 degrees C. The relative timings of myotube and muscle fibre formation were similar, with respect to somite stage, at all three temperatures. Myogenesis was seen to begin medially, adjacent to the notochord, and also in separate zones located near the outer surface of the myotomes, believed to be the sites of formation of future slow muscle fibres. Temperature did not affect the relative timings of most aspects of neural development, including HNK-1-immunoreactivity of myosepta, primary motor neuron axonogenesis, Rohon-Beard dendrite outgrowth, and expression of acetylcholinesterase in the spinal chord and at the myosepta. The posterior progression of the lateral line primordium was slightly but significantly delayed relative to somite stage in embryos reared at 10 degrees C compared to 6 and 2 degrees C, while formation of vacuoles in the notochord occurred relatively earlier at higher temperatures. No significant differences in neuromuscular development were observed between offspring of migratory and of non-migratory females.
Killeen
J R
JR
Gatty Marine Laboratory, School of Environmental and Evolutionary Biology, University of St. Andrews, Fife, UK. james-killeen@hotmail.com
McLay
H A
HA
Johnston
I A
IA
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D018959:CD57 Antigens / Q000032:analysis; D016014:Linear Models; D009046:Motor Neurons / Q000737:chemistry / Q000201:enzymology; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000201:enzymology; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology*; D009672:Notochord / Q000166:cytology; D019170:Somites / Q000166:cytology; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*; D013696:Temperature; D014337:Trout / Q000196:embryology*; D014617:Vacuoles
D018959:CD57 Antigens; D000110:Acetylcholinesterase