Delineation of genomic deletion in cardiomyopathic hamster.
Cardiomyopathic hamster is a representative animal model for autosomal recessive cardiomyopathy. We have previously shown that the transcript of delta-sarcoglycan is missing in the heart of cardiomyopathic hamster due to genomic deletion. Here we define the normal genomic region deleted in cardiomyopathic hamster, which spans about 30 kb interval and includes the two first exons of the delta-sarcoglycan gene. RNA blot analysis using genomic DNA fragments covering the entire deletion as probes failed to detect any transcript other than delta-sarcoglycan in normal hamster heart, suggesting that delta-sarcoglycan is the only transcript defective in the heart of cardiomyopathic hamster.
Sakamoto
A
A
Department of Bioscience, Research Institute, National Cardiovascular Center, Suita, Osaka, Japan.
Abe
M
M
Masaki
T
T
D000818:Animals; D001483:Base Sequence; D009202:Cardiomyopathies / Q000235:genetics*; D006224:Cricetinae / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D005091:Exons; D015870:Gene Expression; D005808:Genes, Recessive; D016678:Genome; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009137:Muscular Dystrophy, Animal / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D020133:Reverse Transcriptase Polymerase Chain Reaction; D049031:Sarcoglycans; D017384:Sequence Deletion
D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D049031:Sarcoglycans