title: Quality of reinfused drainage blood after total knee arthroplasty.
abstract: Reinfusion of postoperative wound drainage blood has become an attractive alternative in primary total knee and hip arthroplasty. Quality of the drainage blood was studied with respect to content of extracellular bioactive substances and coagulation split products. Using the HandyVac ATS autotransfusion system, drainage blood was collected and reinfused within 6 hours postoperatively from 10 patients undergoing primary total knee arthroplasty. Blood samples were collected from the patients immediately after and 1 hour after opening of the tourniquet and after reinfusion of drainage blood. Samples were also collected from the drainage blood immediately before and at the end of reinfusion. The leukocyte-derived and platelet-derived bioactive substances histamine, eosinophil cationic protein (ECP), eosinophil protein X (EPX), myeloperoxidase (MPO), plasminogen activator inhibitor type 1 (PAI-1), and activated complement factor C3(C3a) and various coagulation factors and split products were analyzed in patient and drainage blood samples. None of the patients received additional predonated autologous blood or allogeneic blood components during the study period. Within 6 hours postoperatively, 250 to 1,000 mL drainage blood was collected and reinfused. Histamine, ECP, EPX, MPO, PAI-1, and C3a content was significantly increased in drainage blood immediately before and at the end of reinfusion. Reinfusion did not change the concentration of these substances in samples from the patients. Coagulation factors and various split products showed that drainage blood was defibrinated. Reinfusion of drainage blood did not change the coagulative capacity of the patients. Drainage blood appears to be defibrinated and contains various extracellular leukocyte-derived and platelet-derived bioactive substances. Reinfusion does not change the coagulative capacity or the concentration of bioactive substances of patients.
authors.affiliation: Department of Orthopaedic Surgery, Copenhagen County Hospital, University of Copenhagen, Gentofte, Denmark.
authors.forename: C M
authors.initials: CM
authors.lastname: Jensen
authors.forename: R
authors.initials: R
authors.lastname: Pilegaard
authors.forename: K
authors.initials: K
authors.lastname: Hviid
authors.forename: J D
authors.initials: JD
authors.lastname: Nielsen
authors.forename: H J
authors.initials: HJ
authors.lastname: Nielsen
mesh_terms: D019645:Arthroplasty, Replacement, Knee; D001779:Blood Coagulation Factors / Q000032:analysis; D016063:Blood Loss, Surgical; D001804:Blood Transfusion, Autologous / Q000592:standards*; D006801:Humans; D013997:Time Factors
chemical_list: D001779:Blood Coagulation Factors