Determination of hydroxyaromatic compounds in water by solid-phase microextraction coupled to high-performance liquid chromatography.
Solid-phase microextraction (SPME) coupled with high-performance liquid chromatography (HPLC) for the analysis of hydroxyaromatic compounds is described. Three kinds of fibers [50 microns carbowax-templated resin (CW-TPR), 60 microns polydimethylsiloxane-divinylbenzene (PDMS-DVB) and 85 microns polyacrylate (PA) fibers] were evaluated. CW-TPR and PDMS-DVB were selected for further study. The parameters of the desorption procedure (such as desorption mode, the composition of the solvent for desorption and the duration of fiber soaking) were studied and optimized. The effect of the structure and physical properties of analytes, carryover, duration of absorption, temperature of absorption, pH and ionic strength of samples were also investigated. The method was applied to environmental samples (lake water) using a simple calibration curve.
Wu
Y C
YC
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Huang
S D
SD
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D005618:Fresh Water / Q000032:analysis*; D006841:Hydrocarbons, Aromatic / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D009994:Osmolar Concentration; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006841:Hydrocarbons, Aromatic; D014874:Water Pollutants, Chemical