title: Expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor on B-1a cell from persistent lymphocytosis (PL) cows and lymphoma cell induced by bovine leukemia virus.
abstract: The effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on B lymphocytes from persistent lymphocytosis (PL) cattle and lymphoma cells induced by bovine leukemia virus (BLV) was studied in vitro. Flow cytometric analysis showed that high levels of receptors to GM-CSF were expressed on these cell types. Proliferation of these B cells was induced in response to bovine GM-CSF. In tumor cell lines, the rate of cell proliferation was correlated with expression of GM-CSF receptors. A monoclonal antibody to GM-CSF inhibited lymphocyte proliferation and blocked the GM-CSF binding of lymphocytes. Cells expressing GM-CSF receptor were Ig positive and both CD5 and CD11 positive (B-1a cell). These results suggest that an abnormal expression of GM-CSF receptors on B lymphocytes from PL and lymphoma cells induced by BLV plays important roles in the PL and proliferation of lymphoma.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan. kenji.murakami@yale.edu
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Inumaru
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry*; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D005260:Female; D008213:Lymphocyte Activation; D008218:Lymphocytosis / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor