Sigmoid model versus median-effect analysis for obtaining dose-response curves for in vitro chemosensitivity testing.
OBJECTIVE
The aim of this study was to determine how the in vitro dose-response effects of chemotherapeutic agents should be analyzed and reported.


METHODS
We arbitrarily evaluated the effects of mitomycin-C and 4-OH-cyclophosphamide on the human ovarian cancer cell line CAOV-3. Dose-response curves (DRCs) were calculated by non-linear (sigmoid model: SigmaPlot) and linear curve fitting (median-effect analysis: CalcuSyn).


RESULTS
In theory and practice, the sigmoid model provided better curve fits and graphical presentation than the median-effect analysis.


CONCLUSIONS
Thus, this model should preferably be used as a basis for selection of anti-cancer drugs for clinical use.
Pöch
G
G
Department of Pharmacology and Toxicology, University of Graz, Austria.
Vychodil-Kahr
S
S
Petru
E
E
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D016014:Linear Models; D016685:Mitomycin / Q000008:administration & dosage*; D015233:Models, Statistical; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; C012358:4-hydroxycyclophosphamide; D016685:Mitomycin; D003520:Cyclophosphamide