Characterization of anti-heart antibodies in mice after infection with coxsackie B3 virus.
Coxsackie virus B3 (CVB3) infection results in a marked inflammatory response and the production of autoantibodies to cardiac antigens, with cardiac myosin heavy chain documented to be the most immunogenic antigen. The present study investigated the temporal appearance of anti-heart antibodies in mice after mock infection or infection with an attenuated variant of CVB3 or wildtype CVB3 by SDS-PAGE and Western blotting. Further characterization of the autoantigens was carried out using 2D electrophoresis followed by Western blotting. Mice infected with wildtype CVB3 demonstrated high levels of IgG anti-heart antibodies, reacting predominantly with myosin heavy chain but also with numerous other myocardial proteins. Significant increases in anti-myosin heavy chain, anti-actin, and anti-tropomyosin antibodies were seen in wildtype-infected mice as early as day 7 postinfection compared to those mice that were mock infected or infected with attenuated virus. Characterization of other antigens revealed novel reactivities against myosin subfragments, heat shock proteins, and desmin and its subfragments.
Latif
N
N
Division of Cardiothoracic Surgery, Imperial College of Science, Technology and Medicine, Harefield Hospital, Middlesex, United Kingdom.
Zhang
H
H
Archard
L C
LC
Yacoub
M H
MH
Dunn
M J
MJ
D000199:Actins / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000302:isolation & purification; D003384:Coxsackievirus Infections / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D029822:Enterovirus B, Human / Q000472:pathogenicity; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D051379:Mice; D009205:Myocarditis / Q000209:etiology / Q000276:immunology / Q000473:pathology; D009206:Myocardium / Q000276:immunology*; D018995:Myosin Heavy Chains / Q000276:immunology; D013997:Time Factors; D014335:Tropomyosin / Q000276:immunology; D014774:Virulence
D000199:Actins; D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D014335:Tropomyosin; D018995:Myosin Heavy Chains