Human T lymphocyte populations which bind to P- or E-selectin are enriched with cells expressing core 2 O-glycans.
The present study was undertaken to investigate whether core 2 O-glycans are involved in binding of resting human T lymphocytes to P- or E-selectin and in recruitment of these cells to inflammatory sites. Freshly isolated human peripheral blood T lymphocytes were incubated with P- or E-selectin-coated dishes, and expression of core 2 O-glycans by the adherent and nonadherent cells was examined using the anti-1D4 mAb, which specifically recognizes human CD43 modified with core 2 O-glycans. The results indicated that both the P-selectin/adherent and E-selectin/adherent populations were significantly enriched with ID4+ cells, as compared with the initial population. An enrichment of ID4+ cells in the P- and E-selectin/adherent populations was observed in both CD4 and CD8 T cell subsets and even in the CD45RO+ memory CD4 T-cell subset. However, the anti-1D4 mAb did not inhibit binding of human T lymphocytes to P- or E-selectin, indicating that the 1D4 antigen itself is not directly involved in selectin binding. We also found that the percentage of ID4+ cells in synovial fluid T lymphocytes of rheumatoid arthritis patients was significantly increased as compared with normal peripheral blood T lymphocytes. Taken together, our results support the notion that core 2 O-glycans, which are located apart from the ID4 antigen, are involved in binding of human resting T lymphocytes to both P- and E-selectin, and these interactions may contribute to preferential recruitment of human memory CD4 T lymphocytes to inflammatory sites, including the synovium of rheumatoid arthritis patients.
Mukasa
R
R
Department of Clinical Immunology and AIDS Research Center, Institute of Medical Science, University of Tokyo, Japan.
Homma
T
T
Hosono
O
O
Yoshino
S
S
Nishioka
K
K
Fukuda
M
M
Morimoto
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology; D002448:Cell Adhesion; D019040:E-Selectin / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D019007:P-Selectin / Q000276:immunology* / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D019007:P-Selectin; D011134:Polysaccharides