Markers of pulmonary inflammation in tracheobronchial fluid of premature infants with respiratory distress syndrome.
To asses the role of pulmonary inflammation in the outcome of preterm neonates with respiratory distress syndrome (RDS) we measured soluble intercellular adhesion molecule-1 (ICAM-1), interleukin-8 (IL-8), eosinophil cationic protein (ECP) and transforming growth factor beta-1 (TGF beta 1) in tracheobronquial lavage (TBL) fluid of 15 preterm infants; 9/15 completely recovered from RDS and 6/15 developed chronic lung disease (CLD). ICAM-1 (p: 0.001) and TGF beta 1 (p: 0.04) levels increased in TBL fluid during the first days of life. The values of ICAM-1 were correlated to the days of 0(2) and mechanical ventilation dependency. At 3 days of age, ICAM-1 levels in TBL fluid were higher in infants who later developed CLD compared to infants without CLD (24.5 vs 8.3 micrograms/ml; p: 0.02). Thereafter no significant differences were found although the CLD group had higher values. IL-8 levels showed a fall, specially from 1 to 3 days of age in children without CLD (77.0 to 41.7 ng/ml) although not significant. No difference in TGF beta 1 values were found between both groups, but the TGF beta 1 levels were lower in patients with CLD and they showed undetectable values in 8 samples. ICAM-1 is a major factor associated with airways inflammation whereas IL-8 is not a good marker during the first days of life to predict the RDS outcome. A defect of TGF beta 1 in the smallest premature infants may delay the lung repair process which occurs after tissue injury. High ICAM-1 levels and low TGF beta 1 levels in lung fluid are related to oxygen dependency at 28 days of age.
Burón
E
E
Dpt. of Paediatrics, Hospital Clínico, University of Valladolid, Spain.
Garrote
J A
JA
Arranz
E
E
Oyágüez
P
P
Fernández Calvo
J L
JL
Blanco Quirós
A
A
D015415:Biomarkers; D001798:Blood Proteins / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D047091:Eosinophil Granule Proteins; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D011014:Pneumonia / Q000150:complications*; D012127:Respiratory Distress Syndrome, Newborn / Q000150:complications / Q000473:pathology*; D012260:Ribonucleases; D014132:Trachea; D016212:Transforming Growth Factor beta / Q000032:analysis; D016896:Treatment Outcome
D015415:Biomarkers; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D016209:Interleukin-8; D016212:Transforming Growth Factor beta; D018799:Intercellular Adhesion Molecule-1; D012260:Ribonucleases