Interleukin-10 in combination with interferon-gamma and tumor necrosis factor-alpha enhances in vitro production of nitric oxide by murine resident paritoneal macrophage.
Resident peritoneal macrophages obtained from CBA/J mice were cultured in the presence of recombinant interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and interleukin-10 (IL-10). IFN-gamma-stimulated nitric oxide (NO) formation, IL-10 substantially enhanced the production of NO generated by IFN-gamma + TNF-alpha, irrespective of the timing and dosing of any of the three cytokines. Increased levels of iNOS mRNA after the triple combination of IFN-gamma + TNF-alpha + IL-10 versus IFN-gamma + TNF-alpha suggest that the NO-enhancing effect is mediated, at the least partially, at the transcriptional level. Collectively, the present data support the view that IL-10 is not a general macrophage deactivating factor or a suppressor of gene expression.
Zidek
Z
Z
Institute of Pharmacology, Academy of Sciences of the Czech Republic, Vídexnská 1083, 142 20 Prague 4, Czech Republic.
Franková
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D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse