title: Expression of the genes for guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus is regulated by the two component signal transduction system PhoP-PhoR in B. subtilis.
abstract: Promoters of the genes for guanyl-specific ribonucleases, secreted by B. intermedius (binase) and B. pumilus (Rnase Bp) in phosphate deficient conditions, contain regions similar to appropriate consensus sequences in promoters of the PHO regulated genes of B. subtilis. A number of genes expressed in response to phosphate starvation in B. subtilis are regulated by the two component signal transduction system PhoP-PhoR. Expression of recombinant genes for binase and RNase Bp in B. subtilis strains with mutations in the regulatory protein genes of the PHO regulon was studied. Their expression is strongly regulated by the regulatory proteins of the B. subtilis PHO regulon.
authors.affiliation: Department of Microbiology, Kazan State University, Russia. lilia.znamenskaya@ksu.ru
authors.forename: L V
authors.initials: LV
authors.lastname: Znamenskaya
authors.forename: O A
authors.initials: OA
authors.lastname: Vershinina
authors.forename: V I
authors.initials: VI
authors.lastname: Vershinina
authors.forename: I B
authors.initials: IB
authors.lastname: Leshchinskaya
authors.forename: R W
authors.initials: RW
authors.lastname: Hartley
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004722:Endoribonucleases; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D018085:Regulon; D006163:Ribonuclease T1 / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C061279:PhoR protein, Bacteria; C061064:PhoP protein, Bacteria; D004722:Endoribonucleases; C022543:ribonuclease T(2); D006163:Ribonuclease T1