title: pFab-CMV, a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies.
abstract: We have constructed a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies and their expression in eukaryotic cells. This vector, named pFab-CMV, utilizes the same unique cloning sites present on the pComb3 phagemid thus allowing for the direct subcloning of light chains and heavy chain Fd regions. pFab-CMV also allows for the expression of recombinant Fabs in eukaryotic cells by removal of a cassette containing part of the hinge, CH2 and CH3 sequences. Stable cell lines are rapidly obtained with pFab-CMV by NEO selection without the need for co-transfection of heavy and light chain expressing vectors.
authors.affiliation: Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA. psanna@scripps.edu
authors.forename: P P
authors.initials: PP
authors.lastname: Sanna
authors.forename: M E
authors.initials: ME
authors.lastname: Samson
authors.forename: J S
authors.initials: JS
authors.lastname: Moon
authors.forename: R
authors.initials: R
authors.lastname: Rozenshteyn
authors.forename: A
authors.initials: A
authors.lastname: De Logu
authors.forename: R A
authors.initials: RA
authors.lastname: Williamson
authors.forename: D R
authors.initials: DR
authors.lastname: Burton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001435:Bacteriophages / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D019151:Peptide Library; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019151:Peptide Library; D011994:Recombinant Proteins