Cutaneous uptake of 14C-HD vapor by the hairless guinea pig.
The hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD), bis(2-chloroethylsulfide), exposure. We determined the HD content in the skin of HGP after a 7-min exposure to vapors saturated with a mixture of HD and 14C-HD. Concentration/time (CT) values in the range of 2 micrograms/cm2/min were determined by counting skin 14C disintegrations per min (dpm) in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hr post- exposure. This curve showed the greatest change after 1 hr. The epidermal (62%) to dermal (38%) ratio of 14C at 24 hr was measured for two animals. We saw no site preference for HD penetration among the 8 sites used. The 14C content of template adhesive tape was determined to follow HD distribution. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.
Logan
T P
TP
United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425, USA.
Millard
C B
CB
Shutz
M
M
Schulz
S M
SM
Lee
R B
RB
Bongiovanni
R
R
D000818:Animals; D002250:Carbon Radioisotopes; D002273:Carcinogens / Q000493:pharmacokinetics* / Q000633:toxicity; D002619:Chemical Warfare Agents / Q000493:pharmacokinetics* / Q000633:toxicity; D003879:Dermatologic Agents / Q000493:pharmacokinetics* / Q000633:toxicity; D005740:Gases; D006168:Guinea Pigs; D008297:Male; D009151:Mustard Gas / Q000493:pharmacokinetics* / Q000633:toxicity; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption / Q000187:drug effects
D002250:Carbon Radioisotopes; D002273:Carcinogens; D002619:Chemical Warfare Agents; D003879:Dermatologic Agents; D005740:Gases; D009151:Mustard Gas