Sonodynamically induced cell damage with 4'-O-tetrahydropyranyladriamycin, THP.
BACKGROUND
4'-O-tetrahydropyranyladriamycin (THP) is a novel anthracycline derivative with cardiotoxicity significantly lower than ADM. In this study, the ultrasonically induced in vitro cell damaging effect of THP was investigated.


METHODS
Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound for up to 60 s in the presence and absence of THP. The viability of the isolated cells was determined by staining of the cells with Trypan Blue dye.


RESULTS
The rate of inducing cell damage with ultrasound was doubled with 80 microM THP, while no cell damage was observed with THP alone.


CONCLUSIONS
The enhancement of ultrasonically induced in vitro cell damage was demonstrated with THP. This enhancement was significantly inhibited by histidine, which may suggest a sonochemical mechanism.
Yumita
N
N
School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Kaneuchi
M
M
Okano
Y
Y
Nishigaki
R
R
Umemura
K
K
Umemura
S
S
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005609:Free Radicals; D006639:Histidine / Q000494:pharmacology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D012510:Sarcoma 180 / Q000628:therapy; D013482:Superoxide Dismutase; D014464:Ultrasonic Therapy
D000903:Antibiotics, Antineoplastic; D005609:Free Radicals; D008353:Mannitol; D006639:Histidine; D004317:Doxorubicin; C027260:pirarubicin; D013482:Superoxide Dismutase