title: A novel method for automatic functional annotation of proteins.
abstract: BACKGROUND
  To cope with the increasing amount of sequence data, reliable automatic annotation tools are required. The TrEMBL database contains together with SWISS-PROT nearly all publicly available protein sequences, but in contrast to SWISS-PROT only limited functional annotation. To improve this situation, we had to develop a method of automatic annotation that produces highly reliable functional prediction using the language and the syntax of SWISS-PROT.
  
  
  RESULTS
  An algorithm was developed and successfully used for the automatic annotation of a testset of unknown proteins. The predicted information included description, function, catalytic activity, cofactors, pathway, subcellular location, quaternary structure, similarity to other protein, active sites, and keywords. The algorithm showed a low coverage (10%), but a high specificity and reliability.
  
  
  BACKGROUND
  The results can be obtained by anonymous ftp from ftp.ebi.ac.uk/pub/databases/sp_tr_nrdb. The source code is available on request from the authors.
authors.affiliation: The EMBL Outstation - The European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK. fleischmann@ebi.ac.uk
authors.forename: W
authors.initials: W
authors.lastname: Fleischmann
authors.forename: S
authors.initials: S
authors.lastname: Möller
authors.forename: A
authors.initials: A
authors.lastname: Gateau
authors.forename: R
authors.initials: R
authors.lastname: Apweiler
mesh_terms: D000465:Algorithms; D016208:Databases, Factual; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D015203:Reproducibility of Results; D012984:Software
chemical_list: D011506:Proteins