Transcriptionally active and inactive mouse beta-globin gene loci are repaired at similar rates after ultraviolet irradiation.
It has been demonstrated, by Northern blot and in situ hybridization, that the mouse erythroleukaemia cell line F4N-Sofia constitutively expresses the beta-globin genes. The recently developed quantitative assay for DNA repair has been used to study the overall repair rate in the beta-globin gene domain in this cell line after ultraviolet irradiation and to compare it with the repair rate of the same chromatin domain in mouse Ehrlich ascites tumour cells which do not express the beta-globin genes. The results showed that in both cases the 5'-end of the domain was repaired preferentially and that the repair rates in the two cell lines were very similar despite the different transcription state of the genes.
Chakalova
L
L
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Russev
G
G
D000818:Animals; D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D004260:DNA Repair; D005914:Globins / Q000235:genetics*; D051379:Mice; D005810:Multigene Family; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D017931:DNA Primers; D005914:Globins