In vitro antisickling activity of a rearranged limonoid isolated from Khaya senegalensis.
We previously observed that aqueous extracts of the stem bark and leaves of Khaya senegalensis exhibited a strong antisickling activity. These results prompted us to find out the constituent(s) responsible for these properties using an in vitro bio-guided fractionation. The bioassay was based on sickle cells countings, before and after deoxygenation, in blood samples taken from patients with severe sickle cell anemia and pre-incubated with the drugs to be tested. The main active constituent was identified as a rearranged limonoid whose structure was recently elucidated. In comparison with pentoxifylline used as standard, the in vitro antisickling activity of this limonoid was much higher at any concentrations and incubation conditions. In addition, it did not alter significantly the corpuscular indices.
Fall
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Laboratoire de Pharmacognosie et Bromatologie, l'Université Libre de Bruxelles, U.L.B., Belgium.
Vanhaelen-Fastré
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R
Vanhaelen
M
M
Lo
I
I
Toppet
M
M
Ferster
A
A
Fondu
P
P
D000293:Adolescent; D000328:Adult; D000986:Antisickling Agents / Q000737:chemistry / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D003374:Coumarins / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007223:Infant; D036701:Limonins; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
D000986:Antisickling Agents; D001952:Bridged-Ring Compounds; D003374:Coumarins; D036701:Limonins; D010936:Plant Extracts; C119279:methyl 1,2,3,6,8,14-hexahydroxy(4,2,1(10,30),1(1,4))tricyclomeliac-7-oate