title: Enrichment of peripheral blood CD34+ cells for transplantation using a fully automated immunomagnetic cell selection system and a novel octapeptide releasing agent.
abstract: Positive selection of CD34+ cells is being increasingly performed to support hematological reconstitution following high-dose and dose-intensive chemotherapy and to reduce the non-target cell content of transplants. The present study was designed to evaluate the performance of an immunomagnetic cell selection system, including comparison of enzyme and peptide releasing agents and of semi-automated and fully automated selection systems. A total of 74 immunomagnetic CD34+ cell selection procedures were performed involving 55 subjects, the majority of whom had hematologic malignancies. Median CD34+ cell purity with a newly developed specific octapeptide releasing agent (98.5%; 81.0-99.0%) was significantly higher (P = 0.002) than that with chymopapain (85.8%; 28.1-99.7%). No significant differences were observed between semi-automated and fully automated systems in CD34+ cell purity or yield or time to WBC or platelet recovery. Immunomagnetic selection was found to provide highly purified populations of CD34+ cells in sufficient numbers for use in transplantation procedures. CD34+ cell transplants supported rapid and reliable hematologic reconstitution. Use of a fully automated system markedly reduced the time and labor required for immunomagnetic selection, potentially affording more standardized and reproducible positive selection of CD34+ cells.
authors.affiliation: Etablissement de Transfusion Sanguine de L'Assistance Publique-Hôpitaux de Paris, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Marolleau
authors.forename: L
authors.initials: L
authors.lastname: Dal Cortivo
authors.forename: B
authors.initials: B
authors.lastname: Mills
authors.forename: J P
authors.initials: JP
authors.lastname: Fermand
authors.forename: J M
authors.initials: JM
authors.lastname: Miclea
authors.forename: J P
authors.initials: JP
authors.lastname: Lotz
authors.forename: C
authors.initials: C
authors.lastname: Gisselbrecht
authors.forename: M
authors.initials: M
authors.lastname: Benbunan
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D001781:Blood Component Removal; D002917:Chymopapain / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018189:Immunomagnetic Separation; D008297:Male; D008875:Middle Aged; D009842:Oligopeptides / Q000494:pharmacology*; D012189:Retrospective Studies
chemical_list: D018952:Antigens, CD34; D009842:Oligopeptides; D002917:Chymopapain