title: Dominant infiltration of T(H)1-type CD4+ T cells at the retrobulbar space of patients with thyroid-associated ophthalmopathy.
abstract: Lymphocyte infiltration in the retrobulbar space is a prominent histological feature of thyroid-associated ophthalmopathy (TAO). We have characterized phenotypic and functional features of T cells derived from retrobulbar infiltrates of 3 TAO patients to better understand their roles in the disease. One hundred four T-cell clones (TCC) were directly established from cells of retrobulbar tissues using a highly efficient cloning procedure. Phenotypic analysis of TCC showed approximately 70% to 80% were CD3+ CD4+ CD8- T cells, and approximately 20% to 30% were CD3+ CD8+ CD4- T cells. None of the TCC were CD3+ CD4- CD8- T cells. Analysis of the cytokine profile of TCC, as documented by the ability to express interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 demonstrated that the majority of TCC expressed T helper (T(H))1-like profile in both the mRNA and protein levels. A few TCC showed T(H)0-like profile, but no TCC showed T(H)2-like profile. These results suggest that T(H)1-type CD4+ T cells play important roles in the pathogenesis of TAO.
authors.affiliation: Department of Medicine, Kurume University School of Medicine, Asahimachi, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Hiromatsu
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Nonaka
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D016516:CD4-CD8 Ratio; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006111:Graves Disease / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008222:Lymphokines / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009915:Orbit / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018417:Th1 Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D008222:Lymphokines