Induced expression of CYP2A5 in inflamed trematode-infested mouse liver.
Trematode infections have long been associated with specific types of cancer. We investigated the ability of the liver fluke Fasciola hepatica to alter host enzymes in a manner that might provide insight into the phenomenon of biologically associated cancers. Our data demonstrate an increased activity of the CYP2A5 isozyme in male mouse liver infected with F.hepatica. Induction of this enzyme was further assessed immunohistochemically. The infection affected CYP2A5 distribution in hepatic tissue. Inflammation and proliferation in liver tissue were observed at the same time that CYP2A5 activity increased. This enzyme is known to participate in the metabolism of several carcinogens which are common contaminants in environments of developing countries where parasitic infections may be prevalent.
Montero
R
R
Instituto de Investigaciones Biomédicas, UNAM, México City, DF, México.
Gentile
G J
GJ
Frederick
L
L
McMannis
J
J
Murphy
T
T
Silva
G
G
Blankespoor
H
H
Gentile
J M
JM
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D065637:Cytochrome P-450 CYP2A6; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D000072467:Cytochrome P450 Family 2; D004198:Disease Susceptibility; D004790:Enzyme Induction; D005211:Fascioliasis / Q000201:enzymology*; D007150:Immunohistochemistry; D008099:Liver / Q000033:anatomy & histology / Q000469:parasitology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D013997:Time Factors; D014468:Umbelliferones / Q000097:blood / Q000494:pharmacology
D014468:Umbelliferones; C031477:7-hydroxycoumarin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; C585520:Cyp2a5 protein, mouse; D065637:Cytochrome P-450 CYP2A6; D000072467:Cytochrome P450 Family 2; D010634:Phenobarbital