title: Cloning of oxetanocin A biosynthetic and resistance genes that reside on a plasmid of Bacillus megaterium strain NK84-0128.
abstract: Bacillus megaterium strain NK84-0218 produces a potent antiviral antibiotic, oxetanocin A, which has an oxetanosyl-N-glycoside linkage to an adenine moiety. However, the oxetanocin A productivity of the original strain was unstable and low. In this study, oxetanocin A productivity and resistance was shown to be lost simultaneously when a 51.5-kb plasmid, pOXT1, was cured during cultivation. The deficiency of oxetanocin A productivity and resistance was restored by re-introduction of the pOXT1 plasmid into the cured strain. By a cloning experiment it was shown that a 6.8-kb BglI-D fragment of the pOXT1 plasmid was responsible for oxetanocin A productivity and resistance.
authors.affiliation: Research and Development Division, Nippon Kayaku Co. Ltd., Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Tomita
authors.forename: M
authors.initials: M
authors.lastname: Ishizawa
authors.forename: K
authors.initials: K
authors.lastname: Takagi
authors.forename: F
authors.initials: F
authors.lastname: Kawamura
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Morino
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000998:Antiviral Agents / Q000378:metabolism*; D001410:Bacillus megaterium / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D009154:Mutation; D010957:Plasmids / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; C051061:oxetanocin; D000225:Adenine