Partial protection of mice against Trypanosoma cruzi after immunizing with the TcY 72 antigenic preparation.
A 72 kDa Trypanosoma cruzi glycoprotein recognized by the 164C11 monoclonal antibody (IgM isotype) was purified by preparative electrophoresis. The antigenic preparation obtained, named TcY 72, was used to immunize C57Bl/10 mice. The following results were observed after immunization: (1) induction of higher titres of IgG than IgM antibodies, as evaluated by indirect immunofluorescence; (2) significant DTH after injection of epimastigotes in mice footpads; (3) peak parasitemia in immunized mice was significantly reduced and animals were negative by 13 days post-infection, although the mice still succumb to infection; (4) the phenotypic analysis of spleen cell populations showed a decrease in the CD4/CD8 ratio in immunized mice. Taken as a whole, these findings indicate that TcY 72 is immunogenic and potentially important for protective immunity.
Gomes
Y M
YM
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE, 50670-420, Brasil. yara@cpqam.fiocruz.br
Abath
F G
FG
Nakazawa
M
M
Minoprio
P
P
Vouldoukis
I
I
Monjour
L
L
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000276:immunology*; D005260:Female; D006023:Glycoproteins; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D006023:Glycoproteins