title: Identification of betaIII- and betaIV-tubulin isotypes in cold-adapted microtubules from Atlantic cod (Gadus morhua): antibody mapping and cDNA sequencing.
abstract: Isolated microtubule proteins from the Atlantic cod (Gadus morhua) assemble at temperatures between 8 and 30 degrees C. The cold-adaptation is an intrinsic property of the tubulin molecules, but the reason for it is unknown. To increase our knowledge of tubulin diversity and its role in cold-adaptation we have further characterized cod tubulins using alpha- and beta-tubulin site-directed antibodies and antibodies towards posttranslationally modified tubulin. In addition, one cod brain beta-tubulin isotype has been sequenced. In mammals there are five beta-tubulins (betaI, betaII, betaIII, betaIVa and betaIVb) expressed in brain. A cod betaIII-tubulin was identified by its electrophoretic mobility after reduction and carboxymethylation. The betaIII-like tubulin accounted for more than 30% of total brain beta-tubulins, the highest yield yet observed in any animal. This tubulin corresponds most probably with an additional band, designated beta(x), which was found between alpha- and beta-tubulins on SDS-polyacrylamide gels. It was found to be phosphorylated and neurospecific, and constituted about 30% of total cod beta-tubulin isoforms. The sequenced cod tubulin was identified as a betaIV-tubulin, and a betaIV-isotype was stained by a C-terminal specific antibody. The amount of staining indicates that this isotype, as in mammals, only accounts for a minor part of the total brain beta-tubulin. Based on the estimated amounts of betaIII- and betaIV-tubulins in cod brain, our results indicate that cod has at least one additional beta-tubulin isotype and that beta-tubulin diversity evolved early during fish evolution. The sequenced cod betaIV-tubulin had four unique amino acid substitutions when compared to beta-tubulin sequences from other animals, while one substitution was in common with Antarctic rockcod beta-tubulin. Residues 221, Thr to Ser, and 283, Ala to Ser, correspond in the bovine tubulin dimer structure to loops that most probably interact with other tubulin molecules within the microtubule, and might contribute to cold-adaptation of microtubules.
authors.affiliation: Department of Zoology/Zoophysiology, Göteborg University, Sweden. carina.modig@zool.gu.se
authors.forename: C
authors.initials: C
authors.lastname: Modig
authors.forename: P E
authors.initials: PE
authors.lastname: Olsson
authors.forename: I
authors.initials: I
authors.lastname: Barasoain
authors.forename: C
authors.initials: C
authors.lastname: de Ines
authors.forename: J M
authors.initials: JM
authors.lastname: Andreu
authors.forename: M C
authors.initials: MC
authors.lastname: Roach
authors.forename: R F
authors.initials: RF
authors.lastname: Ludueña
authors.forename: M
authors.initials: M
authors.lastname: Wallin
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D019143:Evolution, Molecular; D005399:Fishes / Q000378:metabolism*; D015723:Gene Library; D008099:Liver / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D010063:Ovum / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D014404:Tubulin; D017239:Paclitaxel