Human members of the SCO1 gene family: complementation analysis in yeast and intracellular localization.
Cytochrome c oxidase is a multiprotein complex in the mitochondrial membrane whose biogenesis requires a number of proteins besides the structural subunits. Several yeast proteins as well as a human disease-related protein have been reported which are involved in cytochrome c oxidase assembly. The S. cerevisiae Sco1p protein has been implicated in the transfer of copper to cytochrome c oxidase subunits Cox1p and/or Cox2p. Here we report on the complementation behavior in yeast of two recently identified ScSco1p homologs of chromosome 17 and chromosome 22 from human. When allotropically expressed in yeast, both genes fail to complement the lack of the ScSCO1 gene. However, a chimera of the N-terminal half of ScSco1p and the C-terminal half of the chromosome 17 homolog does substitute for the ScSco1p function. Interestingly, the respective chimera with the human homolog of chromosome 22 is not able to complement. Expression of EGFP fusions in HeLa cells shows that both human ScSco1p homologs are located in the mitochondria of human cells.
Paret
C
C
Institut für Genetik, Technische Universität Dresden, Germany.
Ostermann
K
K
Krause-Buchholz
U
U
Rentzsch
A
A
Rödel
G
G
D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000302:isolation & purification; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry*; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D008164:Luminescent Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D011993:Recombinant Fusion Proteins; C066384:SCO1 protein, S cerevisiae; C489345:SCO1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D003576:Electron Transport Complex IV