Overexpression of c-Src enhances cell-matrix adhesion and cell migration in PDGF-stimulated NIH3T3 fibroblasts.
c-Src is normally associated with the plasma membrane, but upon activation by tyrosine kinase receptors it translocates to the cytoskeleton. Activation of c-Src alters its conformation and induces the association of c-Src with cytoskeletal proteins. c-Src is implicated in tyrosine phosphorylation of cytoskeletal proteins, which might affect the cytoskeletal architecture. Rearrangements of the cytoskeleton affect cell-matrix adhesion and cell migration. In this study NIH3T3 fibroblasts, that overexpress c-Src, were used to analyze the effect of c-Src on both cell-matrix adhesion and cell migration. Upon PDGF stimulation translocation of c-Src to the cytoskeleton was detected. PDGF treatment also increased cell-matrix adhesion and cell migration. The cell line with the highest c-Src expression showed the largest increases in both phenomena. These findings suggest that translocation of c-Src to the cytoskeleton results in enhanced cell-matrix adhesion and cell migration.
Verbeek
B S
BS
Department of Hematology, University Hospital Utrecht, Utrecht, 3508 GA, The Netherlands. B.S.Verbeek@med.uu.nl
Vroom
T M
TM
Rijksen
G
G
D016475:3T3 Cells; D000818:Animals; D001692:Biological Transport; D002448:Cell Adhesion; D002451:Cell Compartmentation; D002465:Cell Movement; D002471:Cell Transformation, Neoplastic; D003599:Cytoskeleton; D005109:Extracellular Matrix; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D016392:Proto-Oncogene Proteins pp60(c-src)