Comparison of three commercial systems for the identification of germ-tube negative yeast species isolated from clinical specimens.
Three commercial systems were evaluated for their ability to identify 171 germ-tube negative yeasts isolated from clinical specimens. The Yeast Biochemical Card and Analytical Profile Index 20 AUX identified 97% of 171 strains tested. The Biolog system had poor clinical utility: only 48% of strains were identified. For Yeast Biochemical Card and Analytical Profile Index 20 AUX, 9% and 6%, respectively, required repeat testing and both systems required supplemental tests for 28% of the strains. These observations indicate that considerable expertise and a battery of reagents in addition to the basic systems are required for accurate identification of germ-tube negative yeasts.
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National Centre for Mycology, University of Alberta Hospital, Edmonton, Canada.
Rennie
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D008828:Microbiological Techniques; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification