Conformational variability in Escherichia coli 70S ribosome as revealed by 3D cryo-electron microscopy.
During protein biosynthesis, ribosomes are believed to go through a cycle of conformational transitions. We have identified some of the most variable regions of the E. coli 70S ribosome and its subunits, by means of cryo-electron microscopy and three-dimensional (3D) reconstruction. Conformational changes in the smaller 30S subunit are mainly associated with the functionally important domains of the subunit, such as the neck and the platform, as seen by comparison of heat-activated, non-activated and 50S-bound states. In the larger 50S subunit the most variable regions are the L7/L12 stalk, central protuberance and the L1-protein, as observed in various tRNA-70S ribosome complexes. Difference maps calculated between 3D maps of ribosomes help pinpoint the location of ribosomal regions that are most strongly affected by conformational transitions. These results throw direct light on the dynamic behavior of the ribosome and help in understanding the role of these flexible domains in the translation process.
Agrawal
R K
RK
Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA.
Lata
R K
RK
Frank
J
J
D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D020285:Cryoelectron Microscopy / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry / Q000648:ultrastructure*; D006358:Hot Temperature; D008958:Models, Molecular; D011487:Protein Conformation; D012333:RNA, Messenger / Q000737:chemistry; D012335:RNA, Ribosomal / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure*
D001426:Bacterial Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal