title: The human cationic antimicrobial protein (hCAP18), a peptide antibiotic, is widely expressed in human squamous epithelia and colocalizes with interleukin-6.
abstract: Peptide antibiotics are widespread in nature and, by providing a rapid first line of defense, may be key players in the innate immune system. Although epithelia are the main barriers shielding the internal environment from microorganisms, the role for peptide antibiotics in epithelial protection is unclear. We recently reported that the human cationic antimicrobial protein hCAP18, the precursor of the antimicrobial peptide called LL-37, is not expressed by normal human keratinocytes but is induced in various inflammatory skin disorders. In the present study we demonstrate that hCAP18 is consistently expressed at both mRNA and protein levels in squamous epithelia of the mouth, tongue, esophagus, cervix, and vagina in humans. The gene for hCAP18 contains promoter elements that are potentially regulated by interleukin-6, and our data further show a colocalization between interleukin-6 and hCAP18 expression in these tissues. Our finding that hCAP18 is widely produced in squamous epithelia suggests a role for this peptide in epithelial antimicrobial defense. Furthermore, colocalization with interleukin-6 indicates a potential local mechanism for the upregulation of hCAP18 at the epithelial surfaces.
authors.affiliation: Departments of Dermatology, Karolinska Hospital, S-17176 Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Frohm Nilsson
authors.forename: B
authors.initials: B
authors.lastname: Sandstedt
authors.forename: O
authors.initials: O
authors.lastname: Sørensen
authors.forename: G
authors.initials: G
authors.lastname: Weber
authors.forename: N
authors.initials: N
authors.lastname: Borregaard
authors.forename: M
authors.initials: M
authors.lastname: Ståhle-Bäckdahl
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins / Q000378:metabolism*; D054804:Cathelicidins; D002584:Cervix Uteri / Q000276:immunology / Q000378:metabolism; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D004947:Esophagus / Q000276:immunology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000378:metabolism*; D009055:Mouth / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014059:Tongue / Q000276:immunology / Q000378:metabolism; D014621:Vagina / Q000276:immunology / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D054804:Cathelicidins; D015850:Interleukin-6; D012333:RNA, Messenger