Mutational analysis identifies a five base pair cis-acting sequence essential for GBP130 promoter activity in Plasmodium falciparum.
Here we describe the functional characterization of a Plasmodium falciparum promoter region, identifying a discrete five base pair sequence element that is responsible for efficient promoter activity. This sequence element binds nuclear factors in a sequence-specific manner. It shares no homology with any known eukaryotic transcription factor binding site, supporting the notion that the protozoan parasite P. falciparum has evolved a transcriptional machinery distinct from that of its human and mosquito hosts. This report represents the first description of a minimal and necessary cis-acting sequence element for efficient promoter activity in P. falciparum.
Horrocks
P
P
Zentrum für Infektionsforschung der Universität Würzburg, Germany.
Lanzer
M
M
D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D004252:DNA Mutational Analysis; D016054:DNA, Protozoan / Q000032:analysis; D004742:Enhancer Elements, Genetic; D017353:Gene Deletion; D006021:Glycophorins / Q000378:metabolism; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D016054:DNA, Protozoan; D006021:Glycophorins; D015800:Protozoan Proteins