title: Differential mRNA expression and subcellular locations of PI3-kinase isoforms in sympathetic and sensory neurons.
abstract: Phosphatidylinositol 3-kinase (PI3-kinase) enzymes are key signalling molecules in the PC12 and neuronal cell survival pathway and are also involved in the regulation of retrograde axonal transport of nerve growth factor (NGF), with sympathetic neurons more sensitive to the effects of wortmannin/LY294002 than sensory neurons (Bartlett et al. [1997]; Brain Res. 761:257-262; Reynolds et al. [1998] Brain Res. 798:67-74). In this article, we characterized the mRNA expression of PI3-kinase isoforms in mouse sympathetic superior cervical ganglia (SCG) and sensory trigeminal ganglia (TGG) and examined the subcellular locations of immunoreactivity of the PI3-kinase isoforms in mouse cultured SCG and dorsal root ganglion (DRG) neurons. Both the SCG and the TGG express mRNA for the p110alpha, beta, gamma, delta, and vps34p PI3-kinase isoforms, but the TGG and not the SCG express mRNA for the p170 PI3-kinase isoform. In cultured SCG and DRG neurons, p110alpha, beta, and gamma immunoreactivity is in the SCG and DRG growth cones, and predominantly in puncta throughout the growth cone varicosity. However, in the cell bodies immunoreactivity varied, p110alpha is localized predominantly at the plasma membrane, while p110beta and gamma is localized in the perinuclear region of the cells. In addition, unlike other cell types, wortmannin has little effect on actin filament polymerization in either mouse cultured SCG or DRG neurons.
authors.affiliation: Developmental Neurobiology, Division of Neuroscience, The John Curtin School of Medical Research, Australian National University, Canberra, ACT. selena.bartlett@anu.edu.au
authors.forename: S E
authors.initials: SE
authors.lastname: Bartlett
authors.forename: A J
authors.initials: AJ
authors.lastname: Reynolds
authors.forename: T
authors.initials: T
authors.lastname: Tan
authors.forename: K
authors.initials: K
authors.lastname: Heydon
authors.forename: I A
authors.initials: IA
authors.lastname: Hendry
mesh_terms: D000199:Actins / Q000378:metabolism; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002531:Cerebellum / Q000201:enzymology; D015638:Cytochalasin D / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology*; D009475:Neurons, Afferent / Q000201:enzymology*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology; D017783:Superior Cervical Ganglion / Q000201:enzymology; D014158:Transcription, Genetic / Q000187:drug effects; D012668:Trigeminal Ganglion / Q000201:enzymology; D000077191:Wortmannin
chemical_list: D000199:Actins; D000730:Androstadienes; D007527:Isoenzymes; D012333:RNA, Messenger; D015638:Cytochalasin D; D019869:Phosphatidylinositol 3-Kinases; D000077191:Wortmannin