Brachyury regulatory region active in embryonal carcinoma P19 cells.
Brachyury (T) is involved in mesoderm induction during early mouse development. We analyzed the region regulating expression of T in embryonal carcinoma P19 cells, which differentiate into mesoderm derivatives in vitro. Transfection of plasmids encoding reporter genes under the control of the 5'-flanking region showed positive regulatory elements between -298 and -129 bp are responsible for driving T expression in mesodermal cells.
Yamaguchi
H
H
Graduate Program for Biochemical Regulation, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Kitagawa
Y
Y
Miki
K
K
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005326:Fetal Proteins / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D008648:Mesoderm / Q000166:cytology / Q000201:enzymology; D051379:Mice; D020825:T-Box Domain Proteins; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D000098908:Brachyury Protein
D015500:Chloramphenicol O-Acetyltransferase; D004268:DNA-Binding Proteins; D005326:Fetal Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D000098908:Brachyury Protein