Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants.
A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4 x 10(-6) to 1 x 10(-5) transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid.
Geisenberger
O
O
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany. eberl@mikro.biologie.tu-muenchen.de
Ammendola
A
A
Christensen
B B
BB
Molin
S
S
Schleifer
K H
KH
Eberl
L
L
D000333:Aeromonas / Q000235:genetics* / Q000302:isolation & purification; D003227:Conjugation, Genetic; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D017404:In Situ Hybridization, Fluorescence; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D015345:Oligonucleotide Probes; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012722:Sewage / Q000382:microbiology*
D005819:Genetic Markers; D008164:Luminescent Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D012722:Sewage; D049452:Green Fluorescent Proteins