Memory B lymphocytes from secondary lymphoid organs interact with E-selectin through a novel glycoprotein ligand.
Recirculation of B lymphocytes through the secondary lymphoid organs is key for recognition and response to foreign antigen. B lymphocytes within secondary lymphoid organs comprise a heterogeneous population of cells at distinct differentiation stages. To ascribe a particular adhesive behavior to discrete B-cell subsets within secondary lymphoid organs, we investigated their functional interaction with endothelial selectins under flow. We describe herein the characterization of a subset of human tonsillar B cells that interact with E-selectin but not P-selectin. E-selectin-interacting B cells had a phenotype of non-germinal center (CD10(-), CD38(-), CD44(+)), memory (IgD-) cells. Furthermore, FucT-VII was expressed selectively in CD44(+) E-selectin-adherent B lymphocytes. B-cell rolling on E-selectin required sialic acid but was independent of previously described selectin ligands. A novel glycoprotein ligand of 240 kDa carrying N-linked glycans was isolated from B-cell membranes by an E-selectin immunoadhesin. Binding of this protein was strictly Ca2+ dependent, was inhibited by a cell adhesion-blocking mAb against E-selectin, and required the presence of sialic acid but not N-linked carbohydrates. Our results enable us to assign to resident memory B lymphocytes a novel adhesion function, the rolling on E-selectin, that provides insights on the adhesion pathways involved in homing of memory B cells to tertiary sites.
Montoya
M C
MC
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid 28006, Spain.
Holtmann
K
K
Snapp
K R
KR
Borges
E
E
Sánchez-Madrid
F
F
Luscinskas
F W
FW
Kansas
G
G
Vestweber
D
D
de Landázuri
M O
MO
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D016466:CHO Cells; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006224:Cricetinae; D017931:DNA Primers; D019040:E-Selectin / Q000276:immunology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007156:Immunologic Memory; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D014066:Palatine Tonsil / Q000276:immunology*; D010641:Phenotype; D011485:Protein Binding
D017931:DNA Primers; D019040:E-Selectin; D006023:Glycoproteins; D019158:N-Acetylneuraminic Acid