Identification of pituitary adenylate cyclase activating polypeptide (PACAP) and PACAP type 1 receptor in human skin: expression of PACAP-38 is increased in patients with psoriasis.
Morphological and biochemical evidence is presented for the presence of pituitary adenylate cyclase activating peptide (PACAP) and the high-affinity PACAP-1 receptor subtype in human skin. Immunohistochemical analysis revealed PACAP-immunoreactivity (IR) to be present predominantly in dermal nerve fibers close to the dermal-epidermal border, hair follicles, blood vessels and sweat glands. Radioimmunoassay, chromatographic analysis and Western blotting revealed this PACAP-IR to be PACAP-38 whereas the second molecular form, PACAP-27, is absent. In tissue of psoriasis patients significantly more PACAP-38 protein was detected as compared to normal skin. Using RT-PCR, the expression of a high-affinity PACAP-1 receptor in human skin was observed. These results indicate a possible role for PACAP-38 in inflammatory processes of psoriasis.
Steinhoff
M
M
Department of Surgery and Physiology, University of California (UCSF), Medical Center, San Francisco 94143-0660, USA. mstein1@itsa.ucsf.edu
McGregor
G P
GP
Radleff-Schlimme
A
A
Steinhoff
A
A
Jarry
H
H
Schmidt
W E
WE
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000276:immunology / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011565:Psoriasis / Q000378:metabolism*; D011863:Radioimmunoassay; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D011979:Receptors, Pituitary Hormone / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*
C495401:ADCYAP1 protein, human; D000911:Antibodies, Monoclonal; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D011979:Receptors, Pituitary Hormone