title: Effects of a leucocyte depleting arterial line filter on perioperative proteolytic enzyme and oxygen free radical release in patients undergoing aortocoronary bypass surgery.
abstract: BACKGROUND
  Proteolytic enzymes and oxygen free radicals released from activated leucocytes contribute significantly to the organ dysfunction associated with cardiopulmonary bypass. Leucocyte depletion during extracorporeal circulation should reduce the release of these toxic compounds and thereby improve postbypass myocardial and pulmonary function. Recently, a leucocyte-specific arterial line filter to achieve leucocyte depletion during clinical perfusion has become commercially available. The aim of this study, therefore, was to evaluate the influence of the leucocyte depleting arterial line filter on proteolytic enzyme release, oxygen free radical release and postbypass pulmonary and myocardial function in patients undergoing bypass surgery.
  
  
  METHODS
  Forty patients undergoing elective aortocoronary bypass surgery were included into this prospective, randomized clinical study, 20 in the leucocyte depletion (LG-6 group, leucocyte-specific arterial line filter) and 20 in the control group (AV-6 group, standard arterial line filter). White cell count, differential white cell count, plasma elastase concentration, plasma malondialdehyde concentration and C-reactive protein were determined before, twice during and immediately after cardiopulmonary bypass, at the end of surgery and 6 and 20 h thereafter.
  
  
  RESULTS
  White cell count, differential white cell count, malondialdehyde and C-reactive protein were not significantly different between LG-6 and control patients. Plasma elastase concentrations were significantly (P < or = 0.03) higher during and immediately after extracorporeal circulation in LG-6 group patients. Need for inotropic support, arterial pO2 after extracorporeal circulation and perioperative CK MB mass and troponin I release were not different between the two groups of patients.
  
  
  CONCLUSIONS
  The use of a leucocyte depleting arterial line filter is associated with an increased release of the proteolytic enzyme elastase, but does not reliably and consistently achieve effective leucocyte depletion during clinical perfusion. In contrast to previous studies, we could not demonstrate any significant difference in postbypass pulmonary or myocardial function between patients perfused with the leucocyte-specific arterial line filter and control patients. Our data do not support the routine use of a leucocyte depleting arterial line filter during clinical perfusion in patients undergoing elective aortocoronary bypass surgery.
authors.affiliation: Department of Anaesthesia & Intensive Care Medicine, University of Innsbruck, School of Medicine, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Mair
authors.forename: C
authors.initials: C
authors.lastname: Hoermann
authors.forename: J
authors.initials: J
authors.lastname: Mair
authors.forename: J
authors.initials: J
authors.lastname: Margreiter
authors.forename: B
authors.initials: B
authors.lastname: Puschendorf
authors.forename: D
authors.initials: D
authors.lastname: Balogh
mesh_terms: D000368:Aged; D002097:C-Reactive Protein / Q000032:analysis; D002315:Cardiopulmonary Bypass; D002316:Cardiotonic Agents / Q000627:therapeutic use; D001026:Coronary Artery Bypass; D003402:Creatine Kinase / Q000097:blood; D017558:Elective Surgical Procedures; D005112:Extracorporeal Circulation; D005260:Female; D005374:Filtration / Q000295:instrumentation; D005500:Follow-Up Studies; D005609:Free Radicals / Q000097:blood; D006321:Heart / Q000503:physiopathology; D006801:Humans; D007527:Isoenzymes; D007937:Leukapheresis / Q000295:instrumentation*; D007958:Leukocyte Count; D008168:Lung / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010100:Oxygen / Q000097:blood; D010196:Pancreatic Elastase / Q000097:blood; D010447:Peptide Hydrolases / Q000097:blood*; D011446:Prospective Studies; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019210:Troponin I / Q000097:blood
chemical_list: D002316:Cardiotonic Agents; D005609:Free Radicals; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D019210:Troponin I; D008315:Malondialdehyde; D002097:C-Reactive Protein; D003402:Creatine Kinase; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase; D010100:Oxygen