title: Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland.
abstract: A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).
authors.affiliation: Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK. gordon.allan@dani.gov.uk
authors.forename: G M
authors.initials: GM
authors.lastname: Allan
authors.forename: F
authors.initials: F
authors.lastname: Mc Neilly
authors.forename: B M
authors.initials: BM
authors.lastname: Meehan
authors.forename: S
authors.initials: S
authors.lastname: Kennedy
authors.forename: D P
authors.initials: DP
authors.lastname: Mackie
authors.forename: J A
authors.initials: JA
authors.lastname: Ellis
authors.forename: E G
authors.initials: EG
authors.lastname: Clark
authors.forename: E
authors.initials: E
authors.lastname: Espuna
authors.forename: N
authors.initials: N
authors.lastname: Saubi
authors.forename: P
authors.initials: P
authors.lastname: Riera
authors.forename: A
authors.initials: A
authors.lastname: Bøtner
authors.forename: C E
authors.initials: CE
authors.lastname: Charreyre
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000503:physiopathology / Q000662:veterinary*; D017925:Circovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D003718:Denmark; D005838:Genotype; D009660:Northern Ireland; D016133:Polymerase Chain Reaction; D013030:Spain; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology* / Q000821:virology; D019282:Wasting Syndrome / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology
chemical_list: D017931:DNA Primers