title: Quantification of RNA in HIV type 1 subtypes D and G by NucliSens and Amplicor assays in Abidjan, Ivory Coast.
abstract: We have compared the performance of the NucliSens and the standard and modified HIV Monitor assays to quantify HIV-1 RNA plasma viral load in 12 tuberculosis patients infected with HIV-1 env subtype D (n = 3) and env subtype G (n = 9) in Ivory Coast. RNA was quantified in all nine subtype G specimens by the modified Amplicor HIV Monitor (mean, 4.6 log10 copies/ml; range, 3.1-6.3 log10/ml), in seven specimens by NucliSens (mean, 4.4 log10 copies/ml; range, 2.7-5.5 log10 copies/ml), and in 6 specimens by the standard Amplicor HIV Monitor assay (mean, 4.2 log10 copies/ml; range, 3.5-5.0 log10 copies/ml). All three subtype D samples were amplified by both the modified Amplicor HIV Monitor (mean, 4.5 log10 copies/ml; range, 3.8-5.1 log10 copies/ml) and NucliSens (mean, 3.8 log10 copies/ml; range, 2.8-5.0 log10 copies/ml); two samples were quantified by the standard Amplicor HIV Monitor assay (mean, 3.0 log10 copies/ml; range, 2.4-3.6 log10 copies/ml). Our preliminary results suggest that the modified Amplicor HIV Monitor can accurately quantify HIV-1 RNA viral load in persons infected with subtype D and G strains.
authors.affiliation: Projet RETRO-CI, CHU Treichville, Abidjan, Ivory Coast. jcn5@cdc.gov
authors.forename: J N
authors.initials: JN
authors.lastname: Nkengasong
authors.forename: C
authors.initials: C
authors.lastname: Bile
authors.forename: M
authors.initials: M
authors.lastname: Kalou
authors.forename: C
authors.initials: C
authors.lastname: Maurice
authors.forename: E
authors.initials: E
authors.lastname: Boateng
authors.forename: M
authors.initials: M
authors.lastname: Sassan-Morokro
authors.forename: M
authors.initials: M
authors.lastname: Rayfield
authors.forename: D
authors.initials: D
authors.lastname: Coulibaly
authors.forename: A E
authors.initials: AE
authors.lastname: Greenberg
authors.forename: S Z
authors.initials: SZ
authors.lastname: Wiktor
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000097:blood / Q000821:virology*; D007560:Cote d'Ivoire; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D014376:Tuberculosis / Q000097:blood / Q000821:virology*; D019562:Viral Load
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic