Development and evaluation of a 24 well microtitre plate method for isolation of Listeria spp. or Listeria monocytogenes from foods.
A rapid and simple method (24M) using 24 well microtitre plates was developed to determine the presence of Listeria monocytogenes or Listeria spp. in food samples. The 24M was composed of two 24 well microtitre plates connected with a yellow tip. The 24M was evaluated with pathogen cocktails and ground beef samples and compared with the conventional method for presumptive identification of Listeria spp. Only food-borne pathogen cocktails and ground beef samples containing L. monocytogenes or Listeria spp. showed a positive reaction in 24M after 24 h incubation at 35 degrees C. Test results were the same with the conventional method and the 24M method and showed high efficiency for recovery of Listeria spp. from foods. This new, convenient and economical method can isolate Listeria spp. simultaneously from 24 different food samples.
Kang
D H
DH
Department of Animal Sciences and Industry, Kansas State University, Manhattan, USA. Kang@marc.usda.ars
Fung
D Y
DY
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification*; D008461:Meat Products / Q000382:microbiology*
D003470:Culture Media