[Comparison with indocyanine green angiography and immunohistochemical study of choroidal neovascular membrane in age-related macular degeneration].
OBJECTIVE
We classified choroidal neovascular membranes (CNMs) (48 eyes) in age-related macular degeneration (AMD) into four types using indocyanine green angiography.


METHODS
Surgically extracted CNMs were examined immunohistochemically. Specimens were stained with glial fibrillary acidic protein (GFAP), von Willebrand factor, Ki-67 antigen, actin, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF), and transforming growth factor (TGF-beta 1).


RESULTS
All types of CNM were positively stained with GFAP on the side opposite the retinal pigment epithelial (RPE) cells. This suggests the adherence of neurosensory retina to the CNM. Type I membranes which showed hyperfluorescence in the early and late phase of ICG angiography were significantly stained with Ki-67 antigen.


CONCLUSIONS
These results indicate that CNMs in AMD are extracted with neurosensory retina and RPE cells during surgery. Type I membranes have a high capability for proliferation.
Nakajima
M
M
Department of Ophthalmology, Inatori Hospital of Nihon University.
Shimada
H
H
Sato
M
M
Yuzawa
M
M
D020256:Choroidal Neovascularization / Q000378:metabolism / Q000473:pathology*; D016228:Endothelial Growth Factors / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D005451:Fluorescein Angiography; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006651:Histocytochemistry; D007208:Indocyanine Green; D019394:Ki-67 Antigen / Q000032:analysis; D008222:Lymphokines / Q000032:analysis; D008268:Macular Degeneration / Q000378:metabolism / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014841:von Willebrand Factor / Q000032:analysis
D016228:Endothelial Growth Factors; D005904:Glial Fibrillary Acidic Protein; D019394:Ki-67 Antigen; D008222:Lymphokines; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014841:von Willebrand Factor; D016222:Fibroblast Growth Factor 2; D007208:Indocyanine Green