title: Limited and selective localization of the lysosomal membrane glycoproteins LGP85 and LGP96 in rat osteoclasts.
abstract: Monospecific antibodies against two major glycoproteins of rat lysosomal membranes with apparent molecular masses of 96 and 85 kDa, termed LGP96 and LGP85, respectively, were used as probes to determine the expression and distribution of lysosomal membranes in rat osteoclasts. At the light microscopic level, the preferential immunoreactivity for both proteins was found at high levels at the side facing bone of actively bone-resorbing osteoclasts. Osteoclasts detached from bone surface were devoid of immunoreactivity for each protein. At the electron microscopic level, both proteins were exclusively confined to the apical plasma membrane at the ruffled border of active osteoclasts with well-developed ruffled border membrane. No immunolabeling for both proteins was observed in the basolateral membrane and the clear zone of bone-resorbing osteoclasts. The plasma membrane of preosteoclasts and post- and/or resting osteoclasts showed little or no reactivity against these two antibodies. The results indicate that lysosomal membrane glycoproteins are actively synthesized in active osteoclasts, rapidly transported to the ruffled border area, and contribute to the formation and maintenance of the acidic resorption lacuna of osteoclasts.
authors.affiliation: Department of Pharmacology, Kyushu University Faculty of Dentistry, Fukuoka, Japan. kyama@dent.kyushu-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Maeda
authors.forename: K
authors.initials: K
authors.lastname: Akasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimine
authors.forename: A
authors.initials: A
authors.lastname: Akamine
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018955:CD36 Antigens / Q000032:analysis*; D007425:Intracellular Membranes / Q000737:chemistry; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000737:chemistry / Q000648:ultrastructure; D008562:Membrane Glycoproteins / Q000032:analysis*; D010010:Osteoclasts / Q000737:chemistry*; D011817:Rabbits; D051381:Rats
chemical_list: D015703:Antigens, CD; D018955:CD36 Antigens; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; C070693:Scarb2 protein, rat