title: Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence.
abstract: Eleven thousand, three hundred and seventy enhancer/promoter trap lines in Arabidopsis were generated via T-DNA transformation utilizing the binary vector pD991 that contains a minimal promoter fused to the uidA reporter gene. Overall 31% of the lines generated exhibit a staining pattern in the inflorescence. Flanking DNA has been cloned from 15 lines exhibiting inflorescence staining patterns by either thermal asymmetric interlaced PCR (TAIL-PCR), inverse PCR (IPCR), or partial library construction. Seeds from these lines are available from the ABRC and NASC Arabidopsis stock centers and DNA pools are available from the ABRC.
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: L
authors.initials: L
authors.lastname: Campisi
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Yi
authors.forename: E
authors.initials: E
authors.lastname: Heilig
authors.forename: B
authors.initials: B
authors.lastname: Herman
authors.forename: A J
authors.initials: AJ
authors.lastname: Cassista
authors.forename: D W
authors.initials: DW
authors.lastname: Allen
authors.forename: H
authors.initials: H
authors.lastname: Xiang
authors.forename: T
authors.initials: T
authors.lastname: Jack
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D017343:Genes, Plant; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA; D005966:Glucuronidase