Characteristics of the production of active oxygen species from adherent and non-adherent neutrophils.
Our objective was to evaluate the characteristics of the production of AOS from the neutrophils that had adhered to the endothelial cells, fibronectin or polystyrene, using the method of electron paramagnetic resonance (EPR) spin trapping. Neutrophils and endothelial cells were isolated from human venous blood and umbilical veins, respectively. AOS production from neutrophils was not elicited only by adhesion. The stimulation of adherent neutrophils with phorbol myristate acetate (PMA) induced the production of AOS. The production of AOS from adherent neutrophils to endothelial cells, but not to fibronectin or polystyrene, decreased with the interval time between the adhesion and the stimulation by PMA. The amount of AOS produced by the neutrophils adherent to fibronectin or polystyrene was maintained for one hour after stimulation with PMA, whereas that by suspended neutrophils gradually decreased with the time after stimulation. Results indicate that adherent and non-adherent neutrophils exhibit differing time course of AOS production.
Yoshida
N
N
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan. nyoshida@koto.kpu-m.ac.jp
Yoshikawa
T
T
Nakagawa
S
S
Miyajima
T
T
Nakamura
Y
Y
Naito
Y
Y
Tanigawa
T
T
Kondo
M
M
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000032:analysis / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
D003497:Cyclic N-Oxides; D005353:Fibronectins; D007375:Interleukin-1; D011137:Polystyrenes; D017382:Reactive Oxygen Species; D009240:N-Formylmethionine Leucyl-Phenylalanine; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate