title: In vivo comparison of synthetic osseous graft materials. A preliminary study.
abstract: The purpose of this study was to compare the in vivo osseous healing response of 4 commercially-available synthetic bone grafting materials; hydroxylapatite (HA), calcium sulfate (CaSO4) plus autogenous bone, or a bioactive glass ceramic: with particle size of 300-360 microm (BG1) or 90 to 710 microm (BG2). 4 osteotomy sites were prepared in each tibia of 10 adult male rabbits. One unfilled osteotomy site served as negative control (NC) and another site filled with autogenous bone was the positive control (PC). All animals received BG1 in 2 sites and BG2 in 2 sites. 5 animals received HA and five CaSO4 plus autogenous bone in the remaining 2 sites. Animals were sacrificed at 28 days post-surgery, histologic sections obtained and the % surface area of new bone formation for each material was determined by computerized image analysis. All graft sites showed evidence of bone formation, i.e., (NC) 41.95%; (PC) 50.41%; (BG1) 41.82%; (BG2) 40.36%; (HA) 41.83% and (CaSO4) 58.83%. Statistical analysis using an ANOVA with repeated measures on the materials common to all animals (excluding HA and CaSO4 groups) showed significant differences between materials in surface area of bone, with positive controls better than negative controls, and BG1 and BG2 not significantly different from the negative control. These results indicate that synthetic graft materials can support new bone formation in surgically prepared defects. The utility of a rabbit model for studying physiologic osseous turnover and healing is questioned for studies of slowly resorbing synthetic graft materials.
authors.affiliation: Department of Periodontics, School of Dentistry, University of Missouri-Kansas City, 64108, USA. macneills@umkc.edu
authors.forename: S R
authors.initials: SR
authors.lastname: MacNeill
authors.forename: C M
authors.initials: CM
authors.lastname: Cobb
authors.forename: J W
authors.initials: JW
authors.lastname: Rapley
authors.forename: A G
authors.initials: AG
authors.lastname: Glaros
authors.forename: P
authors.initials: P
authors.lastname: Spencer
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D016723:Bone Remodeling / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D016025:Bone Transplantation; D001842:Bone and Bones / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery; D002133:Calcium Sulfate / Q000627:therapeutic use; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use; D004195:Disease Models, Animal; D017886:Durapatite / Q000627:therapeutic use; D007091:Image Processing, Computer-Assisted; D008297:Male; D010012:Osteogenesis / Q000502:physiology; D010027:Osteotomy; D010316:Particle Size; D011817:Rabbits; D013977:Tibia; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D018786:Bone Substitutes; D017886:Durapatite; D002133:Calcium Sulfate