The effect of oxygen radicals on rat thymocyte glucose transport is independent of the site of their generation.
We studied the relationship between the site of production of oxygen radicals and their effect on a rat thymocyte functional activity, the glucose transport, measured using a radioactive analogue of glucose, 2-deoxy-glucose. We compared the effects of a hydrophilic thermolabile azo compound, mimicking a radical attack outside the cell, with the lipid-soluble cumene hydroperoxide, which initiates lipid peroxidation in cell membranes. Our results show that a low grade oxidative stress stimulated glucose uptake rapidly, independently of the site of radical generation. In the presence of the azocompound, glucose uptake increased smoothly, attaining its maximum extent within 1 h. In thymocytes treated with cumene hydroperoxide the rate of glucose transport increased suddenly and remained constant over 1 h. The effects of the radical donors on TBARS production and protein sulfhydryl groups content were also evaluated. In thymocytes treated with the azo derivative no lipid peroxidation was observed, but a slow decrease of protein thiol groups occurred; after the addition of cumene hydroperoxide sulfhydryl groups did not change and TBARS increased significantly. The water-soluble antioxidant Trolox was able to remove the glucose uptake increase induced by the hydrophilic initiator and to delay the loss of membrane integrity.
Fiorentini
D
D
Dipartimento di Biochimica G. Moruzzi, Università di Bologna, Italy.
Hakim
G
G
Zambonin
L
L
Landi
L
L
D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D005609:Free Radicals / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013950:Thymus Gland / Q000166:cytology
D000578:Amidines; D001555:Benzene Derivatives; D005609:Free Radicals; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; C046728:2,2'-azobis(2-amidinopropane); D003513:Cycloheximide; D003847:Deoxyglucose; D005947:Glucose; C007164:cumene hydroperoxide