Transforming growth factor-beta1, a strong costimulator of rat T-cell activation promoting a shift towards a Th2-like cytokine profile.
TGF-beta is a known regulator of hematopoietic cells. In this study we suggest a major role of adherent spleen cells (adh-splc), to convert an inhibitory effect of TGF-beta1 on T-cell activation into a stimulatory effect. We show that interaction of TGF-beta1 with adh-splc induces a costimulatory effect on T-cell proliferation. This costimulatory signal requires the adh-splc to be in physical contact with the T-cells. Presence of adh-splc results in a shift towards a Th2-like response with a cytokine profile of increased IL-10 and decreased IFN-gamma. In the adh-splc population the increase of IL-10 is most pronounced at start of activation, whereas in the T-lymphocyte population, IL-10 increases at the end of culture. The suppression of the IFN-gamma production by TGF-beta1 is shown to be an important mechanism by which TGF-beta enhances proliferation of Th2 lymphocytes.
Schiött
A
A
The Wallenberg Laboratory, Department of Tumor Immunology, University of Lund, Sweden. asa.schiott@wblab.lu.se
Widegren
B
B
Sjögren
H O
HO
Lindvall
M
M
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D016207:Cytokines / Q000096:biosynthesis*; D004768:Enterotoxins / Q000276:immunology; D005557:Formaldehyde; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D016707:Tissue Fixation; D016212:Transforming Growth Factor beta / Q000276:immunology*
D016207:Cytokines; D004768:Enterotoxins; D011108:Polymers; D016212:Transforming Growth Factor beta; D005557:Formaldehyde; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma; C003043:paraform