An intronic domain within the rat preprotachykinin-A gene containing a CCCT repetitive motif acts as an enhancer in differentiating embryonic stem cells.
Previous attempts by several groups to clone fragments containing intron 2 of the rat preprotachykinin-A gene have generated deletions of various sizes. We have determined that these deletions occur within a specific region of the intron spanning a CCCT tandem repeat domain. We show that this intronic domain is able to support reporter gene expression in mouse embryonic stem (ES) cells that have been induced to differentiate but not in undifferentiated ES cells. No significant expression was observed in the HeLa clonal cell line. This demonstrates that this intron 2 domain is a highly restrictive enhancer and may be associated with differentiation.
Fiskerstrand
C E
CE
Department of Veterinary Pathology, University of Edinburgh, UK.
Lovejoy
E
E
Gerrard
L
L
Quinn
J P
JP
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D015320:Tachykinins / Q000235:genetics*
D011498:Protein Precursors; D011994:Recombinant Proteins; D015320:Tachykinins; C044206:preprotachykinin; D008156:Luciferases