Identification of Tn10 insertions in the dsbA gene affecting Escherichia coli biofilm formation.
Escherichia coli was used as model to study initial adhesion and early biofilm development to an abiotic surface. Tn10 insertion mutants with reduced attachment to a polystyrene surface were isolated. Three adhesion mutants harbored the transposon in the dsbA gene, whose product, DsbA, catalyses folding of numerous extracytoplasmic disulfide bond-containing proteins. All three mutants were weakly adherent and grew poorly. Cell surface structure analysis showed that motility. type 1 fimbriation and lipopolysaccharide structure were affected in these mutants. The pleiotropic effect of the dsbA mutations on biofilm formation is discussed.
Genevaux
P
P
Department of Molecular Microbiology, Faculty of Biology, Free University of Amsterdam, The Netherlands. pierre@bio.vu.nl
Bauda
P
P
DuBow
M S
MS
Oudega
B
B
D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000378:metabolism; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D019897:Periplasm; D016133:Polymerase Chain Reaction / Q000379:methods; D011137:Polystyrenes; D019704:Protein Disulfide-Isomerases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013499:Surface Properties
D004251:DNA Transposable Elements; D008070:Lipopolysaccharides; D011137:Polystyrenes; D019704:Protein Disulfide-Isomerases