Heat-shock protein-73 protects against small intestinal warm ischemia-reperfusion injury in the rat.
BACKGROUND
The protective effects of heat-shock protein (hsp) in rat small intestinal warm ischemia-reperfusion (I/R) injury are poorly understood.


METHODS
Hsp-73 expression was induced in rat small intestine with use of sodium arsenite injected (6 mg/kg) through a catheter cannulated into the left common carotid artery 24 hours before ischemia (group 1). In the control group an equal volume of phosphate-buffered saline solution was injected (group 2). To block the induction of hsp-73 expression, sodium arsenate and quercetin (5 mg/kg) were injected (group 3).


RESULTS
The mean peak plasma levels of tumor necrosis factor-alpha and cytokine-induced neutrophil chemoattractant after reperfusion were lower in group 1 than in group 2. The tissue myeloperoxidase activity after reperfusion was lower in group 1 than in group 2. The mean peak plasma level of interleukin-10 after reperfusion was higher in group 1 than in group 2. The induction of hsp-73 expression reduced the synthesis of nitric oxide and the magnitude of the small intestinal warm I/R injury. The results in group 3 were similar to those in group 2.


CONCLUSIONS
Hsp-73 protects against small intestinal warm I/R injury by inhibiting the synthesis of inflammatory cytokines and the activation of neutrophils and by accelerating the synthesis of anti-inflammatory cytokines.
Tsuruma
T
T
Department of Surgery, Sapporo Medical University School of Medicine, Japan.
Yagihashi
A
A
Watanabe
N
N
Yajima
T
T
Kameshima
H
H
Araya
J
J
Hirata
K
K
D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D015153:Blotting, Western; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000097:blood; D006133:Growth Substances / Q000096:biosynthesis / Q000097:blood; D006360:Heat-Shock Proteins / Q000032:analysis / Q000502:physiology*; D006358:Hot Temperature; D036341:Intercellular Signaling Peptides and Proteins; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood; D007413:Intestinal Mucosa / Q000098:blood supply / Q000201:enzymology / Q000601:surgery; D007421:Intestine, Small / Q000098:blood supply* / Q000201:enzymology / Q000601:surgery*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis / Q000097:blood; D009195:Peroxidase / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000139:chemically induced / Q000517:prevention & control*; D017670:Sodium Compounds / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
D018053:Arsenites; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D004791:Enzyme Inhibitors; D006131:Growth Inhibitors; D006133:Growth Substances; D006360:Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D017670:Sodium Compounds; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; C017947:sodium arsenite; D011794:Quercetin; D009195:Peroxidase