title: Exercise and beta-adrenergic regulation of rat cardiac myosin isoforms.
abstract: BACKGROUND
  The purpose of this experiment was to examine the effects of both exercise and beta-adrenergic receptor blockade on the expression of native cardiac myosin isoforms. Specifically, this experiment tested two hypotheses: 1) treatment of sedentary rats with the beta blocker, propranolol, will promote increased ventricular V3 (slow) native myosin content with a concomitant reduction of V1 (fast) myosin isoforms; and 2) endurance exercise training will result in an increased sympathetic drive and therefore will retard the propranolol-induced shift in cardiac myosin isoform expression.
  
  
  METHODS
  Adult, male Sprauge-Dawley rats (120 days old) were randomly divided into 4 groups: 1) exercise-sham (ES); 2) exercise-propranolol (EP); 3) sedentary-sham (SS); and 4) sedentary-propranolol (SP). Propranolol (30 mg drug/kg body wt) and sham (saline) injections (i.p.) were administered 30 minutes prior to daily exercise. Both ES and EP groups completed six weeks (5 day/wk) of treadmill running at approximately 65-70% VO2max.
  
  
  RESULTS
  Data analysis revealed that exercise training did not alter (p > 0.05) ventricular myosin isoforms in the sham injected animals. In contrast, propranolol treatment resulted in a significant (p < 0.05) increase in the slow (V3) myosin isoform and a concomitant decrease in the V1 isoform in both sedentary and exercise trained animals.
  
  
  CONCLUSIONS
  The observed increase in V3 myosin isoform in propranolol treated rats supports the notion that beta-adrenergic stimulation is an important regulator of cardiac myosin isoform expression. However, our hypothesis that exercise training would retard the propranolol-induced shift in cardiac myosin was not supported.
authors.affiliation: Department of Exercise and Sport Science, University of Florida, Gainesville, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Wade
authors.forename: R A
authors.initials: RA
authors.lastname: Herb
authors.forename: S K
authors.initials: SK
authors.lastname: Powers
authors.forename: D
authors.initials: D
authors.lastname: Criswell
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D005069:Evaluation Studies as Topic; D006321:Heart / Q000502:physiology; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D009218:Myosins / Q000737:chemistry* / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011433:Propranolol / Q000494:pharmacology; D020033:Protein Isoforms; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
chemical_list: D000319:Adrenergic beta-Antagonists; D020033:Protein Isoforms; D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D009218:Myosins