Expression of HLA G in human tumors is not a frequent event.
Expression of HLA G may be a way for tumor cells to escape immuno-surveillance. HLA G is selectively expressed by extravillous trophoblast in the human placenta, a tissue that does not express HLA A or B molecules. It is tempting to propose that tumor cells resemble this unique HLA class I phenotype as they frequently lose classical HLA A, B and C class I expression. Such peculiar HLA class I distribution would in theory allow tumor cells to escape from T- and NK-cell cytotoxicity. To determine whether HLA G is expressed on tumor cells, we studied HLA G mRNA levels using RT-PCR and HLA G cell-surface expression by immunohistological techniques in a panel of 50 human solid tumor tissues, 31 tumor cell lines of different origin, 4 autologous mucosa samples and 3 peripheral white cell samples. We found mRNA transcripts of different HLA G isoforms in most of the samples studied. However, we did not detect cell-surface expression of HLA G using 3 specific monoclonal antibodies (MAbs; 87G, 01G and G223). HLA G was detected only in the U937 myelomonocytic cell line after stimulation with IFN-gamma. We favor the hypothesis that HLA G plays a minor role, if any, in providing an inhibitory signal to NK cells to escape immunosurveillance. We cannot, however, exclude the possibility that some other HLA G isoforms may be expressed in some tumors.
Real
L M
LM
Departamento de Análisis Clínicos, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Cabrera
T
T
Collado
A
A
Jimenez
P
P
Garcia
A
A
Ruiz-Cabello
F
F
Garrido
F
F
D000911:Antibodies, Monoclonal; D003110:Colonic Neoplasms / Q000276:immunology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006680:HLA Antigens / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D015234:HLA-A Antigens / Q000032:analysis / Q000235:genetics; D015235:HLA-B Antigens / Q000032:analysis / Q000235:genetics; D015236:HLA-C Antigens / Q000032:analysis / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D010190:Pancreatic Neoplasms; D010920:Placenta / Q000276:immunology; D011247:Pregnancy; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I