Anandamide stimulates phospholipase D activity in PC12 cells but not in NIH 3T3 fibroblasts.
The endogenous cannabinoid arachidonoylethanolamide was previously reported to have no effects on the phospholipase D activity in Chinese hamster ovary cells expressing the human brain-specific cannabinoid receptor, while in mouse peritoneal cells, delta9-tetrahydrocannabinol stimulated this enzyme. In this work, arachidonoylethanolamide (0.1-1 microM) was found to stimulate the phospholipase D-mediated phospholipid hydrolysis in rat adrenal pheochromocytoma PC12 cells, but not in mouse NIH 3T3 fibroblasts. The phospholipase D-activating effects of arachidonoylethanolamide were comparable to those elicited by phorbol ester and nerve growth factor, while arachidonic acid (1 microM) had no effects. The results show that, depending on the cell type, arachidonoylethanolamide can be an activator of the phospholipase D system.
Kiss
Z
Z
Hormel Institute, University of Minnesota, Austin 55912, USA. kissx001@maroon.tc.umn.edu
D016475:3T3 Cells; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D016466:CHO Cells; D002186:Cannabinoids / Q000494:pharmacology*; D006224:Cricetinae; D063388:Endocannabinoids; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D053284:Polyunsaturated Alkamides; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001095:Arachidonic Acids; D002186:Cannabinoids; D063388:Endocannabinoids; D009414:Nerve Growth Factors; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D053284:Polyunsaturated Alkamides; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate; C078814:anandamide