The auxin-binding protein Nt-ERabp1 alone activates an auxin-like transduction pathway.
Hyperpolarization of tobacco protoplasts is amongst the earliest auxin responses described. It has been proposed that the auxin-binding protein, ABP1, or a related protein could be involved in the first step of auxin perception at the plasma membrane. Using for the first time homologous conditions for interaction between the protein Nt-ERabp1 or a synthetic peptide corresponding to the C-terminus and tobacco protoplasts, we have demonstrated that both can induce the hyperpolarization response. The results show that Nt-ERabp1 or the C-terminal peptide alone activates the auxin pathway from the outer face of the plasma membrane.
Leblanc
N
N
Perception and Auxin Transport, Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.
Perrot-Rechenmann
C
C
Barbier-Brygoo
H
H
D000595:Amino Acid Sequence; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010937:Plant Growth Regulators; D010940:Plant Proteins / Q000378:metabolism*; D010947:Plants, Toxic; D011428:Proplast; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction; D014026:Nicotiana; D003313:Zea mays
D007210:Indoleacetic Acids; D010455:Peptides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011428:Proplast; D011956:Receptors, Cell Surface; C046131:auxin receptor, plant