title: Experimental infection of white-tailed deer with rangiferine brucellosis.
abstract: Experimental infections of white-tailed deer (Odocoileus virginianus) with Brucella suis biovar 4 were evaluated over a period of 6 wk. Five adult male hand-raised white-tailed deer were inoculated with 1 x 10(7) colony forming units of B. suis biovar 4 in the conjunctiva and serologically evaluated over 6 wk by the card test (CARD), rivanol test (RIV), serum agglutination test (SAT), complement fixation test (CFT), particle concentration fluorescence immunoassay (PCFIA), and competitive enzyme linked immunosorbent assay (cELISA), as routinely used for bovine samples. Six weeks postinoculation the animals were euthanized and cultured for B. suis biovar 4. One deer became serologically positive 4 wk postinoculation on CFT, CARD, PCFIA, and cELISA. At 6 wk postinoculation, CFT was positive in four infected deer, CARD was positive in three deer; RIV, SAT, and PCFIA was positive in two deer; and cELISA was positive in one deer. Only the CFT was 100% sensitive. At necropsy B. suis biovar 4 was isolated from four of five deer, and representative colonies were biologically similar to the challenge organism.
authors.affiliation: Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: T
authors.initials: T
authors.lastname: Qureshi
authors.forename: J
authors.initials: J
authors.lastname: Stittmatter
authors.forename: K
authors.initials: K
authors.lastname: Turner
authors.forename: D S
authors.initials: DS
authors.lastname: Davis
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002002:Brucella / Q000145:classification / Q000276:immunology / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D003168:Complement Fixation Tests / Q000662:veterinary; D003670:Deer; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D012053:Reindeer; D013154:Spleen / Q000382:microbiology; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial