Determination of cyclosporin A in 20% ethanol by a magnetic beads-based immunofluorescence assay.
A rapid magnetic beads-based immunoassay for the immunodepressant drug cyclosporin A (CsA) has been developed. The method allows CsA determination in medium with a higher content of ethanol compared to conventional immunochemical techniques due to increased antibody stability. Monitoring of the drug in ethanol extracts from patient's whole blood without many-fold dilution with aqueous buffer is possible. The assay has adequate specificity and sensitivity for CsA to be suitable for the routine monitoring of therapy.
Kiselev
M V
MV
Chemistry Department, Moscow State University, Moscow, Russia.
Gladilin
A K
AK
Melik-Nubarov
N S
NS
Sveshnikov
P G
PG
Miethe
P
P
Levashov
A V
AV
D000915:Antibody Affinity; D001774:Blood Chemical Analysis / Q000379:methods* / Q000706:statistics & numerical data; D016572:Cyclosporine / Q000097:blood* / Q000276:immunology; D000431:Ethanol; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood* / Q000276:immunology; D008280:Magnetics; D012680:Sensitivity and Specificity
D007166:Immunosuppressive Agents; D000431:Ethanol; D016572:Cyclosporine