title: A bioseparation apparatus with high-pressure fluid injection and fluid sampling.
abstract: A novel apparatus in which fluids may be injected and sampled at high pressure is described. Bioseparation applications of the apparatus were demonstrated in three model systems: (1) lambdaDNA was eluted under pressure from an anion exchange column into a low-salt (0.25 M) buffer, thereby eliminating conventional time-consuming desalting procedures required for downstream analysis of the DNA; (2) RNA was separated under pressure from a RNA/DNA mixture, thereby enabling rapid differential preparation of nucleic acids; and (3) an antibody was purified from a protein mixture by affinity capture at one pressure and dissociation from the antigen binding partner at a second pressure, thereby enabling the immunoreactivities of both antibody and antigen to be preserved during the separation process.
authors.affiliation: BMA Laboratories, Inc., BioSeq, Inc., 25 Olympia Avenue, Unit F, Woburn, Massachusetts 01801-6307, USA.
authors.forename: G H
authors.initials: GH
authors.lastname: Dreier
authors.forename: F
authors.initials: F
authors.lastname: Tao
authors.forename: R A
authors.initials: RA
authors.lastname: Hess
authors.forename: C Y
authors.initials: CY
authors.lastname: Cheung
authors.forename: L E
authors.initials: LE
authors.lastname: Sciaba
authors.forename: D J
authors.initials: DJ
authors.lastname: Green
authors.forename: J A
authors.initials: JA
authors.lastname: Laugharn
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D010582:Bacteriophage lambda / Q000737:chemistry; D001671:Biochemistry / Q000295:instrumentation*; D002021:Buffers; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004279:DNA, Viral / Q000302:isolation & purification; D006874:Hydrostatic Pressure; D012313:RNA / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D002021:Buffers; D004279:DNA, Viral; D012313:RNA