pFab-CMV, a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies.
We have constructed a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies and their expression in eukaryotic cells. This vector, named pFab-CMV, utilizes the same unique cloning sites present on the pComb3 phagemid thus allowing for the direct subcloning of light chains and heavy chain Fd regions. pFab-CMV also allows for the expression of recombinant Fabs in eukaryotic cells by removal of a cassette containing part of the hinge, CH2 and CH3 sequences. Stable cell lines are rapidly obtained with pFab-CMV by NEO selection without the need for co-transfection of heavy and light chain expressing vectors.
Sanna
P P
PP
Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA. psanna@scripps.edu
Samson
M E
ME
Moon
J S
JS
Rozenshteyn
R
R
De Logu
A
A
Williamson
R A
RA
Burton
D R
DR
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001435:Bacteriophages / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D019151:Peptide Library; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019151:Peptide Library; D011994:Recombinant Proteins