title: Disulfide bridges are not involved in penicillin-binding protein 1b dimerization in Escherichia coli.
abstract: PBP1b can be found as a dimer in Escherichia coli. Previous results suggested that dimerization involved the cysteine(s) in an intermolecular disulfide bond. We show that either deletion mutants or a mutant without cysteines is fully active and still binds penicillin and that the latter can also form dimers.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Chalut
authors.forename: M H
authors.initials: MH
authors.lastname: Remy
authors.forename: J M
authors.initials: JM
authors.lastname: Masson
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins; D003545:Cysteine / Q000235:genetics; D019281:Dimerization; D004220:Disulfides; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006602:Hexosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase; D009154:Mutation; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D046928:Peptidoglycan Glycosyltransferase; D010458:Peptidyl Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004220:Disulfides; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D046928:Peptidoglycan Glycosyltransferase; C478681:penicillin-binding protein 1B, E coli; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; D002267:Muramoylpentapeptide Carboxypeptidase; D003545:Cysteine