title: Antioxidant systems in polymorphonuclear leucocytes of Type 2 diabetes mellitus.
abstract: OBJECTIVE
  To examine the effect of Type 2 diabetes mellitus (DM) on enzymes of importance for oxygen-dependent killing of microorganisms by leucocytes.
  
  
  METHODS
  Twenty patients with Type 2 DM and 20 nondiabetic controls provided blood samples for analysis.
  
  
  RESULTS
  The superoxide dismutase (SOD) activity was lower by 41% in polymorphonuclear leucocytes (PMNL) from patients with Type 2 DM than in controls (3.42+/-0.32 U/mg of protein vs. 5.79+/-0.71 U/mg of protein, P<0.005). Glutathione peroxidase (GSHPx) and glutathione reductase (GR) activities of Type 2 DM patients were 73.04% and 81.12% of control values (0.84+/-0.07 nkat/mg of protein vs. 1.15+/-0.10 nkat/mg of protein, P<0.003, and 2.02+/-0.12 nkat/mg of protein vs. 2.49+/-0.16 nkat/mg of protein, P < 0.023, respectively). The catalase activity showed no significant difference. A significant increase (141.37% of control) in the concentration of thiobarbituric acid reactive products was observed (9.91+/-0.78 miromol/l vs. 7.01+/-0.47 micromol/l, P<0.003). A positive correlation between thiobarbituric acid reactive products and glucose, glycated haemoglobin and fructosamine in the serum of diabetic patients was observed.
  
  
  CONCLUSIONS
  These findings may explain some of the mechanisms underlying the increased susceptibility to certain infection in patients with Type 2 DM.
authors.affiliation: Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Muchová
authors.forename: A
authors.initials: A
authors.lastname: Liptáková
authors.forename: Z
authors.initials: Z
authors.lastname: Országhová
authors.forename: I
authors.initials: I
authors.lastname: Garaiová
authors.forename: P
authors.initials: P
authors.lastname: Tison
authors.forename: J
authors.initials: J
authors.lastname: Cársky
authors.forename: Z
authors.initials: Z
authors.lastname: Duracková
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase