title: Preferential transport of glutathione versus glutathione disulfide in rat liver microsomal vesicles.
abstract: A bi-directional, saturable transport of glutathione (GSH) was found in rat liver microsomal vesicles. GSH transport could be inhibited by the anion transport blockers flufenamic acid and 4, 4'-diisothiocyanostilbene-2,2'-disulfonic acid. A part of GSH taken up by the vesicles was metabolized to glutathione disulfide (GSSG) in the lumen. Microsomal membrane was virtually nonpermeable toward GSSG; accordingly, GSSG generated in the microsomal lumen could hardly exit. Therefore, GSH transport, contrary to previous assumptions, is preferred in the endoplasmic reticulum, and GSSG entrapped and accumulated in the lumen creates the oxidized state of its redox buffer.
authors.affiliation: Institute of General Pathology, University of Siena, 53100 Siena, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bánhegyi
authors.forename: L
authors.initials: L
authors.lastname: Lusini
authors.forename: F
authors.initials: F
authors.lastname: Puskás
authors.forename: R
authors.initials: R
authors.lastname: Rossi
authors.forename: R
authors.initials: R
authors.lastname: Fulceri
authors.forename: L
authors.initials: L
authors.lastname: Braun
authors.forename: V
authors.initials: V
authors.lastname: Mile
authors.forename: P
authors.initials: P
authors.lastname: di Simplicio
authors.forename: J
authors.initials: J
authors.lastname: Mandl
authors.forename: A
authors.initials: A
authors.lastname: Benedetti
mesh_terms: D000408:Alamethicin / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism*; D008027:Light; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation
chemical_list: D000408:Alamethicin; D005978:Glutathione; D019803:Glutathione Disulfide