Lungkine, a novel CXC chemokine, specifically expressed by lung bronchoepithelial cells.
We describe a novel mouse CXC chemokine that is selectively expressed in lung epithelial cells and up-regulated in various lung inflammation models. Although this chemokine clusters with other ELR-CXC chemokines, none of them can confidently be assigned to be its human homologue based on sequence identity. In addition, the highly restricted mRNA tissue distribution of this chemokine differentiates it from all previously described chemokines: Lungkine could not be detected in any of the 70 cDNA libraries analyzed corresponding to specific murine cell populations and tissues. High levels of Lungkine mRNA were specifically detected in the lung and at lower levels in fetal lung tissue by Northern blot and in situ hybridization, suggesting a potential role for this chemokine during lung development. Moreover, Lungkine protein is secreted into the airway spaces and induces the in vitro and in vivo migration of neutrophils, suggesting that it is involved in lung-specific neutrophil trafficking. Using fluorescent in situ hybridization, we show that Lungkine maps to mouse chromosome 5.
Rossi
D L
DL
Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
Hurst
S D
SD
Xu
Y
Y
Wang
W
W
Menon
S
S
Coffman
R L
RL
Zlotnik
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001980:Bronchi / Q000737:chemistry / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D019743:Chemokines, CXC / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004847:Epithelial Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000737:chemistry / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D015854:Up-Regulation / Q000276:immunology
D019743:Chemokines, CXC; C118893:Cxcl15 protein, mouse; D018076:DNA, Complementary; D012333:RNA, Messenger