Role of bio-metal Fe(III) in anticancer effect of dacarbazine.
Physicochemical, Microbial and Pharmacological studies on Fe(III)-Dacarbazine complex have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titrations and IR spectral studies the probable formula for the complex has been worked out to be 1:1, Fe(III)-Dacarbazine. The metal ligand interaction has been studied using polarographic method at 25 +/- 1 degrees C and at ionic strength of mu = 1.0 (KCl). Microbial studies on the complex was done against various pathogenic bacteria viz. Pseudomonas mangiferae, Staphylococcus aureus, Salmonella typhi and Vibrio cholarae and fungi i.e. Trichothecium and Chrysosporium sp. using Raper's method. Mouse sarcoma cell line 180 and Balb/C mice were used for the anticancer screening of solid complex in vitro and in vivo respectively. The observed polarographic data, on lingane treatment revealed the formation of single (1:1) (M:L) complex with Fe(III) and dacarbazine ligands. The results of amperometric titrations of Fe(III) with dacarbazine in IM KCl supporting electrolyte pH 7.0 +/- 0.1 supported the above findings the IR data speaks of the complex formation between the metal and the dacarbazine ligand through the two nitrogen one each of primary amide and trizo groups. The results of microbial and pharmacological studies with the M:Drug complex revealed that the anticancer activity of the drug metal complex is nearly doubled as compared to the pure drug. As such Fe(III) dacarbazine complex may be recommended to the therapeutic experts for its possible use as more potent anticancer drug.
Shukla
J
J
Department of Chemistry, Dr. Harisingh Gour University, Sagar.
Pitre
K S
KS
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D003606:Dacarbazine / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D011048:Polarography; D012513:Sarcoma, Experimental / Q000188:drug therapy; D013055:Spectrophotometry, Infrared; D014407:Tumor Cells, Cultured
D018906:Antineoplastic Agents, Alkylating; D005290:Ferric Compounds; D046911:Macromolecular Substances; D003606:Dacarbazine