title: The glutamate and carbachol effects on the early post-denervation depolarization in rat diaphragm are directed towards furosemide-sensitive chloride transport.
abstract: The membrane potentials of denervated muscle fibres of the rat diaphragm kept in a tissue culture medium are depolarized by about 8-10 mV (10-12%) within 3 h after denervation. This early post-denervation depolarization (EPD) is substantially reduced (2-3 mV) when muscle strips are bathed with 1 mM L-glutamate (GLU) which is found in motor nerve endings, or with 5 x 10(-8) M carbachol (CCh), which mimics the effect of nonquantally released acetylcholine (ACh). The hyperpolarizing effects of GLU and CCh on EPD are not influenced by ouabain, an active sodium transport inhibitor, but are absent when Cl- transport is augmented by increased osmolarity (500 mosmol/l) produced by addition of sucrose or NaCl. The EPD and the effect of hyperosmolarity are effectively prevented by the Cl- transport inhibitor furosemide (1 x 10(-4) M) or by a chloride-free bathing medium. It is suggested that the post-denervation cessation of nonquantal ACh release, and probably also GLU release, from nerve endings leads to the activation of the furosemide-sensitive Cl- transport in the sarcolemma, which is responsible for the early post-denervation depolarization.
authors.affiliation: Department of Physiology, Medical University, Kazan, Russia.
authors.forename: A K
authors.initials: AK
authors.lastname: Urazaev
authors.forename: N V
authors.initials: NV
authors.lastname: Naumenko
authors.forename: E E
authors.initials: EE
authors.lastname: Nikolsky
authors.forename: F
authors.initials: F
authors.lastname: Vyskocil
mesh_terms: D000818:Animals; D001692:Biological Transport; D002217:Carbachol / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism*; D003470:Culture Media; D003964:Diaphragm / Q000294:innervation; D005071:Evoked Potentials; D005665:Furosemide / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000502:physiology*; D018482:Muscle, Skeletal / Q000294:innervation*; D009924:Organ Culture Techniques; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D002712:Chlorides; D003470:Culture Media; D018698:Glutamic Acid; D013779:Tetrodotoxin; D010042:Ouabain; D005665:Furosemide; D002217:Carbachol; D000254:Sodium-Potassium-Exchanging ATPase