title: Conformational studies on the specific cleavage site of Type I collagen (alpha-1) fragment (157-192) by cathepsins K and L by proton NMR spectroscopy.
abstract: Cathepsins K and L are cysteine proteinases which are considered to play an important role in bone resorption. Type I collagen is the most abundant component of the extracellular matrix of bone and regarded as an endogenous substrate for the cysteine proteinases in osteoclastic bone resorption. We have synthesized a fragment of Type I collagen (alpha-1) (157-192) as a substrate for the cathepsins and found that cathepsins K and L cleave the fragment at different specific sites. The major cleavage sites for cathepsin K were Met159-Gly160, Ser162-Gly163 and Arg165-Gly166, while those for cathepsin L were Gly166-Leu167 and Gln180-Gly181. The structure of the fragment was analyzed in aqueous solution by circular dichroism and proton NMR spectroscopy and the difference in the molecular recognition of collagen by cathepsins K and L was discussed from the structural aspect.
authors.affiliation: International Research Laboratories, Ciba-Geigy Japan Ltd., Takarazuka.
authors.forename: A Y
authors.initials: AY
authors.lastname: Nosaka
authors.forename: K
authors.initials: K
authors.lastname: Kanaori
authors.forename: N
authors.initials: N
authors.lastname: Teno
authors.forename: H
authors.initials: H
authors.lastname: Togame
authors.forename: T
authors.initials: T
authors.lastname: Inaoka
authors.forename: M
authors.initials: M
authors.lastname: Takai
authors.forename: T
authors.initials: T
authors.lastname: Kokubo
mesh_terms: D000595:Amino Acid Sequence; D056657:Cathepsin K; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D006801:Humans; D007668:Kidney / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013696:Temperature
chemical_list: D003094:Collagen; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L; C534130:CTSK protein, human; D056657:Cathepsin K