Seroprevalence and disease association of antineutrophil cytoplasmic autoantibodies and antigens in HIV infection.
This prospective study was designed to determine the role of antineutrophil cytoplasmic autoantibodies (ANCA) in HIV-infected patients. Immunofluorescence tests (IFT) and enzyme-linked immunosorbent assays (ELISA) were applied to sera of 199 consecutive outpatients. In the IFT 20% were positive. An atypical ANCA pattern was demonstrated in 67% of these, 33% revealed a perinuclear staining (pANCA). Specific ELISA revealed proteinase 3 (n = 2), myeloperoxidase (n = 1), lysozyme (n = 2), lactoferrin (n = 1), cathepsin G (n = 1), and human leukocyte elastase (HLE, n = 6). The target antigen remained unidentified in 26 patients. Perinuclear ANCA-positive patients showed atypical antigens in eight of 13 cases; all six patients with anti-HLE revealed a pANCA pattern. The antigens of atypical ANCA-positive patients remained unidentified in 21 of 26 (81%) cases. No signs of vasculitis were present in the ANCA-positive patients. ANCA are frequently found in the sera of HIV-positive patients. They bind to a variety of antigens. No correlation was found between ANCA positivity and autoimmune or opportunistic diseases.
Cornely
O A
OA
Klinik I für Innere Medizin, Universitätskliniken Köln, Germany.
Hauschild
S
S
Weise
C
C
Csernok
E
E
Gross
W L
WL
Salzberger
B
B
Fätkenheuer
G
G
Diehl
V
V
Schrappe
M
M
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000097:blood* / Q000302:isolation & purification; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000097:blood; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015658:HIV Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D016036:Seroepidemiologic Studies
D019268:Antibodies, Antineutrophil Cytoplasmic; D000956:Antigens, Viral; D000939:Epitopes