Plasma levels of selectins and interleukins in cardiovascular surgery using cardiopulmonary bypass.
BACKGROUND
Cardiovascular surgery with cardiopulmonary bypass (CPB) leads to activation of a variety of inflammatory pathways, including the release of cytokines and selectins.


METHODS
In 20 patients undergoing elective coronary artery bypass grafting, plasma levels of interleukins IL-2, -6, -8, -10, -12 and of P-, E-, and L-selectins were measured at eight time points before, during, and after CPB using a standardized ELISA technique.


RESULTS
IL-2 plasma levels decreased significantly after the start of CPB and remained low until the second postoperative day. IL-6 and IL-8 levels increased significantly after weaning off CPB, with mean peak values six hours postoperatively. Very low IL-10 plasma levels were detectable preoperatively. They remained low during CPB and peaked significantly after weaning off CPB until skin closure. The IL-12 levels decreased after weaning off CPB (p < 0.05) until 6 hours postoperatively. The plasma levels of P-selectin showed no alterations, but concentrations of E- and L-selectin decreased after the start of CPB (p < 0.05). There were no adverse postoperative events.


CONCLUSIONS
The results of our study demonstrate a dysregulation of cytokine and selectin production during and up to 48 h after CPB, which may be a "normal" stress reaction to CPB.
Sablotzki
A
A
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig University, Giessen, Germany.
Dehne
M G
MG
Mann
V
V
Görlach
G
G
Mühling
J
J
Zickmann
B
B
Hempelmann
G
G
D000328:Adult; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass / Q000379:methods*; D003327:Coronary Disease / Q000097:blood* / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology; D007378:Interleukins / Q000097:blood*; D007432:Intraoperative Period; D011184:Postoperative Period; D011379:Prognosis; D019087:Selectins / Q000097:blood*
D015415:Biomarkers; D007378:Interleukins; D019087:Selectins