V3 serotyping of HIV-1 infection: correlation with genotyping and limitations.
HIV-1 V3 serotyping is a classification of immunodeficiency viruses based on antibody binding to V3 peptides that allows obtaining information on circulating subtypes that could be important for population-based epidemiologic studies. Recently, several laboratories have developed V3 enzyme-immunoassays (EIAs) using V3 peptides of subtypes A to E. In the present study, the utility of including additional peptides of subtypes F to H to the EIA was evaluated on a panel of 203 well-characterized serum samples from patients with diverse geographic origins (22 countries) and known HIV-1 genotype (79 A, 61 B, 21 C, 7 D, 7 E, 21 F, 6 G, 1 H). The results indicate a high predictive value (ppv) for serotypes B (> or =0.86), D (1) and E (0.88), and confirm the difficulty of predicting genotype A or C based on serotype A or C. Results also indicate that inclusion of the F peptide in the V3 EIAs may be useful (ppv = 0.61), but introduction of peptides G and H failed to demonstrate significant sensitivity or specificity for these subtypes. Correlation between serotyping and amino-acid sequences of the V3 region from 103 samples allowed the identification of key amino-acids that appear essential for subtype-specific seroreactivity.
Plantier
J C
JC
Laboratoire de Virologie, Université François Rabelais, Tours, France.
Damond
F
F
Lasky
M
M
Sankalé
J L
JL
Apetrei
C
C
Peeters
M
M
Buzelay
L
L
M'Boup
S
S
Kanki
P
P
Delaporte
E
E
Simon
F
F
Barin
F
F
D000595:Amino Acid Sequence; D005260:Female; D005838:Genotype; D015699:HIV Envelope Protein gp120 / Q000145:classification* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000145:classification* / Q000276:immunology; D012703:Serotyping
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments