title: Chimeric papillomavirus virus-like particles induce a murine self-antigen-specific protective and therapeutic antitumor immune response.
abstract: The use of chimeric virus-like particles represents a new strategy for delivering tumor antigens to the immune system for the initiation of antitumor immune responses. Immunization of DBA/2 mice with the P1A peptide derived from the P815 tumor-associated antigen P1A induced specific T-cell tolerance, resulting in progression of a regressor P815 cell line in all animals. However, immunization with a human papillomavirus type 16 L1 virus-like particle containing the P1A peptide in the absence of adjuvant induced a protective immune response in mice against a lethal tumor challenge with a progressor P815 tumor cell line. Additionally, we demonstrated that these chimeric virus-like particles could be used therapeutically to suppress the growth of established tumors, resulting in a significant survival advantage for chimeric virus-like particle-treated mice compared with untreated control mice. Chimeric virus-like particles can thus be used as a universal delivery vehicle for both tolerizing and antigenic peptides to induce a strong protective and therapeutic antigen-specific antitumor immune response.
authors.affiliation: Cardinal Bernardin Cancer Center, Loyola University-Chicago, Maywood, Illinois 60153, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Nieland
authors.forename: D M
authors.initials: DM
authors.lastname: Da Silva
authors.forename: M P
authors.initials: MP
authors.lastname: Velders
authors.forename: K E
authors.initials: KE
authors.lastname: de Visser
authors.forename: J T
authors.initials: JT
authors.lastname: Schiller
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: W M
authors.initials: WM
authors.lastname: Kast
mesh_terms: D000818:Animals; D001483:Base Sequence; D002678:Chimera; D017931:DNA Primers; D007167:Immunotherapy; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014771:Virion / Q000276:immunology*
chemical_list: D017931:DNA Primers