PsiI, a novel restriction endonuclease recognizing the DNA sequence 5'-TTA downward arrow TAA-3'.
PsiI, a novel restriction endonuclease produced by the bacterial strain Pseudomonas sp. SE-G49, has been isolated and characterized. The enzyme cleaves DNA in the middle of its palindromic recognition sequence 5'-TTA downward arrow TAA-3'. Thus, PsiI belongs to a rare group of type II restriction endonucleases whose recognition sites consist of AT base pairs only.
Abdurashitov
M A
MA
SibEnzyme Co., Novosibirsk, 630117, Russia. se_info@ma.nsc.ru.
Belichenko
O A
OA
Lebedeva
N A
NA
Degtyarev
S K
SK
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006868:Hydrolysis; D013379:Substrate Specificity
D004247:DNA; C120006:endodeoxyribonuclease PsiI; D015252:Deoxyribonucleases, Type II Site-Specific