title: Synthesis of a series of stromelysin-selective thiadiazole urea matrix metalloproteinase inhibitors.
abstract: The synthesis and enzyme inhibition data for a series of thiadiazole urea matrix metalloproteinase (MMP) inhibitors are described. A broad screening effort was utilized to identify several thiadiazoles which were weak inhibitors of stromelysin. Optimization of the thiadiazole leads to include an alpha-amino acid side chain with variable terminal amide substituents provided a series of ureas which were moderately effective stromelysin inhibitors, with Ki's between 0.3 and 1.0 microM. The most effective analogues utilized an L-phenylalanine as the amino acid component. In particular, unsubstituted 46 had a Ki of 710 nM, while the p-fluoro analogue 52 displayed increased potency (100 nM). Stromelysin inhibition was further improved using a pentafluorophenylalanine substituent which resulted in 70, a 14 nM inhibitor. While gelatinase inhibition was generally poor, the use of 1-(2-pyridyl)piperazine as the amide component usually provided for enhanced activity, with 71 inhibiting gelatinase with a Ki of 770 nM. The combination of this heterocycle with a p-fluorophenylalanine substituent provided the only analogue, 69, with collagenase activity (13 microM). The SAR for analogues described within this series can be rationalized through consideration of the X-ray structure recently attained for70 complexed to stromelysin. Uniquely, this structure showed the inhibitor to be completely orientated on the left side of the enzyme cleft. These results suggest that thiadiazole urea heterocycles which incorporate a substituted phenylalanine can provide selective inhibitors of stromelysin. Careful selection of the amide substituent can also provide for analogues with modest gelatinase inhibition.
authors.affiliation: Department of Structural, Analytical and Medicinal Chemistry, Michigan 49007, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Jacobsen
authors.forename: M A
authors.initials: MA
authors.lastname: Mitchell
authors.forename: S K
authors.initials: SK
authors.lastname: Hendges
authors.forename: K L
authors.initials: KL
authors.lastname: Belonga
authors.forename: L L
authors.initials: LL
authors.lastname: Skaletzky
authors.forename: L S
authors.initials: LS
authors.lastname: Stelzer
authors.forename: T J
authors.initials: TJ
authors.lastname: Lindberg
authors.forename: E L
authors.initials: EL
authors.lastname: Fritzen
authors.forename: H J
authors.initials: HJ
authors.lastname: Schostarez
authors.forename: T J
authors.initials: TJ
authors.lastname: O'Sullivan
authors.forename: L L
authors.initials: LL
authors.lastname: Maggiora
authors.forename: C W
authors.initials: CW
authors.lastname: Stuchly
authors.forename: A L
authors.initials: AL
authors.lastname: Laborde
authors.forename: M F
authors.initials: MF
authors.lastname: Kubicek
authors.forename: R A
authors.initials: RA
authors.lastname: Poorman
authors.forename: J M
authors.initials: JM
authors.lastname: Beck
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
authors.forename: G L
authors.initials: GL
authors.lastname: Petzold
authors.forename: P S
authors.initials: PS
authors.lastname: Scott
authors.forename: S E
authors.initials: SE
authors.lastname: Truesdell
authors.forename: T L
authors.initials: TL
authors.lastname: Wallace
authors.forename: J W
authors.initials: JW
authors.lastname: Wilks
authors.forename: C
authors.initials: C
authors.lastname: Fisher
authors.forename: L V
authors.initials: LV
authors.lastname: Goodman
authors.forename: P S
authors.initials: PS
authors.lastname: Kaytes
mesh_terms: D001665:Binding Sites; D005453:Fluorescence; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D008958:Models, Molecular; D011480:Protease Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013830:Thiadiazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D014508:Urea / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D013830:Thiadiazoles; D014508:Urea