title: Influenza A virus RNA polymerase has the ability to stutter at the polyadenylation site of a viral RNA template during RNA replication.
abstract: The viral polymerase of influenza virus, a negative-strand RNA virus, is believed to polyadenylate the mRNAs by stuttering at a stretch of five to seven uridine residues which are located close to the 5' ends of the viral RNA templates. However, a mechanism of polyadenylation based on a template-independent synthesis of the poly(A) tail has not been excluded. In this report, we present new evidence showing the inherent ability of the viral polymerase to stutter at the poly(U) stretch of a viral RNA template during RNA replication. Variants which possess 1- to 13-nucleotide-long insertions at the poly(U) stretch have been identified. These results support a stuttering mechanism for the polyadenylation of influenza virus mRNAs.
authors.affiliation: Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.forename: H A
authors.initials: HA
authors.lastname: Lee
authors.forename: P
authors.initials: P
authors.lastname: Palese
authors.forename: A
authors.initials: A
authors.lastname: García-Sastre
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000502:physiology*; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D014162:Transfection; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D009439:Neuraminidase