title: Chromosome aberrations in adult Hodgkin disease in a Danish population-based study.
abstract: During a 6-year period, 31 patients with Hodgkin disease (HD) were analyzed for chromosome aberrations on lymphoid tissue. We obtained metaphases in 87% (27/31). The number of cells analyzed per case ranged from 17 to 31 (median 25), and the number of abnormal mitoses was between 1 and 17 (median 6). Chromosome aberrations were found in 59% (16/27). Numerical aberrations involved all chromosomes. The most frequently gained chromosomes were numbers 2 and 9, and the most frequently lost were numbers 10, 16, 21, 22, and X. Chromosomes most frequently involved in structural aberrations were numbers 1 and 6. The most frequent subgroups were nodular sclerosis (NS) (n = 16) and mixed cellularity (MC) (n = 10). Six NS patients and 8 patients with MC showed an abnormal clone. For the NS patients with an abnormal karyotype, 4 of 6 had a gain of chromosome 2, and all had structural aberrations of chromosome 1. Of the 6 MC patients, where a partial analysis was possible, 4 had a gain of chromosome 9, 2 had structural aberrations involving chromosome 6 and 2 of chromosome 14. In 1 case a translocation normally associated with non-Hodgkin lymphoma (NHL) was found (t[11;14]), whereas other translocations characteristic of NHL, such as t(8;14), t(14;18), and t(2;5) were not observed. A review of the literature on cytogenetic investigations in HD performed on lymphoid tissue showed that the most frequently gained or lost chromosomes were 1, 2, 5, 9, and 12 for NS and 2, 5, and 9 for MC. The most frequently affected chromosomes in structural aberrations were 1 and 6 for NS, and 1, 7, and 14 for MC. Involvement of chromosome 1, 6, and 14 in structural aberrations is characteristic of lymphoid neoplasms, as are the most frequently involved bands (1p36, 6q21-q26, 14q11, and 14q32) further supporting a B- or T-cell origin of the neoplastic cell in HD. The high hyperploidy seen in HD is not a frequent observation in NHL. Although certain chromosome aberrations seem to be characteristic of HD as opposed to NHL, specific nonrandom aberrations have yet to be identified. The rather low number of abnormal mitoses found in most HD cases underlies the importance of analyzing a large number of metaphases.
authors.affiliation: Institute of Pathology, Odense University Hospital, Denmark.
authors.forename: R K
authors.initials: RK
authors.lastname: Pedersen
authors.forename: A G
authors.initials: AG
authors.lastname: Sørensen
authors.forename: N T
authors.initials: NT
authors.lastname: Pedersen
authors.forename: K G
authors.initials: KG
authors.lastname: Schmidt
authors.forename: G B
authors.initials: GB
authors.lastname: Kerndrup
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000235:genetics*; D003718:Denmark / Q000453:epidemiology; D005260:Female; D005828:Genetics, Population; D006689:Hodgkin Disease / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008677:Metaphase