title: Mechanisms of internalization of Staphylococcus aureus by cultured human osteoblasts.
abstract: Staphylococcus aureus is an important bone pathogen, and evidence shows that this organism is internalized by chick osteoblasts. Here we report that S. aureus is internalized by human osteoblasts. Internalization was inhibited by monodansylcadaverine and cytochalasin D and to a lesser extent by ouabain, monensin, colchicine, and nocodazole. We propose that internalization occurs via a receptor-mediated pathway, requiring the participation of cytoskeletal elements, principally actin.
authors.affiliation: Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Jevon
authors.forename: C
authors.initials: C
authors.lastname: Guo
authors.forename: B
authors.initials: B
authors.lastname: Ma
authors.forename: N
authors.initials: N
authors.lastname: Mordan
authors.forename: S P
authors.initials: SP
authors.lastname: Nair
authors.forename: M
authors.initials: M
authors.lastname: Harris
authors.forename: B
authors.initials: B
authors.lastname: Henderson
authors.forename: G
authors.initials: G
authors.lastname: Bentley
authors.forename: S
authors.initials: S
authors.lastname: Meghji
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D015638:Cytochalasin D / Q000494:pharmacology; D006801:Humans; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000199:Actins; D015638:Cytochalasin D; C008542:monodansylcadaverine; D002103:Cadaverine