Microglial reaction in some CNS nuclei following nerves transection in BALB/c and interferon-gamma gene knockout mice.
The present study was aimed to ascertain if the endogenous IFN-gamma is necessary for induction of microglial reaction associated with the CNS neurons after neurectomy of the vagus and hypoglossal nerves in IFN-gamma gene knockout mice and BALB/c mice serving as controls. Vigorous microglial reaction as detected by Mac-1 antibody was elicited in the brainstem nuclei in both strains of mice. Increased Mac-1 immunoreactivity in microglia was detected as early as 1 day post-operation in the ipsilateral dorsal nucleus of vagus, nucleus ambiguus and the hypoglossal nucleus; a similar feature was observed in the nucleus tractus solitarius bilaterally. Mac-1 immunoreactivity in reactive microglia appeared to increase with survival intervals and was comparable throughout in both strains of mice. It is therefore concluded that endogenous IFN-gamma is not responsible for upregulation of complement type 3 receptor immunomolecules in reactive microglia following nerves sectioning.
Ng
Y K
YK
Department of Anatomy, Faculty of Medicine, National University of Singapore, Singapore.
Yong
V W
VW
Ling
E A
EA
D000818:Animals; D001921:Brain / Q000276:immunology / Q000502:physiology*; D001933:Brain Stem / Q000276:immunology / Q000502:physiology; D007002:Hypoglossal Nerve / Q000502:physiology*; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000032:analysis; D008526:Medulla Oblongata / Q000276:immunology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D017628:Microglia / Q000276:immunology / Q000502:physiology*; D009474:Neurons / Q000276:immunology / Q000502:physiology*; D014630:Vagus Nerve / Q000502:physiology*
D016177:Macrophage-1 Antigen; D007371:Interferon-gamma