Distribution of two hemolytic toxin genes in clinical and environmental isolates of Aeromonas spp.: correlation with virulence in a suckling mouse model.
Previous studies have shown that two hemolytic toxins, HlyA and AerA, contribute to the virulence of Aeromonas hydrophila. A survey was performed to gauge the distribution of hlyA and aerA genes in clinical and environmental Aeromonas isolates. For A. hydrophila, A. veronii biotype sobria and A caviae, 96%, 12% and 35% of strains, respectively, were hlyA positive, whereas, 78%, 97%, 41%, respectively, were aerA positive. All virulent A. hydrophila isolates were hlyA+ aerA+. This genotype was most common in A. hydrophila (75.4%) followed by A. caviae (29.4%) and A. veronii biotype sobria (9.6%). For A. hydrophila, a two-hemolytic toxin model of virulence provides the best prediction of virulence in an animal model.
Heuzenroeder
M W
MW
Infectious Disease Laboratory, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia. heuzenroeder@imvs.sa.gov.au
Wong
C Y
CY
Flower
R L
RL
D000333:Aeromonas / Q000235:genetics* / Q000472:pathogenicity*; D000818:Animals; D000833:Animals, Suckling; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D015139:Blotting, Southern; D002522:Chlorocebus aethiops; D004783:Environmental Microbiology; D029968:Escherichia coli Proteins; D005787:Gene Frequency; D005798:Genes, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D052899:Pore Forming Cytotoxic Proteins; D018675:Toxicity Tests; D014709:Vero Cells
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D052899:Pore Forming Cytotoxic Proteins; C032676:aerolysin