title: Characterization of human intestinal stromal cell lines: response to cytokines and interactions with epithelial cells.
abstract: The maintenance of the physiological homeostasis of the gut mucosa characterized by continuous proliferation and differentiation processes results from epithelial-mesenchymal cell cross-talk. To set out stable and homogeneous models for the study of the (dys)regulation of various morphofunctional aspects, we established and characterized three clonal cell lines (C9, C11, and C20) derived from human duodenal mucosal connective tissue. We defined the expression of (i) cytoskeletal proteins; (ii) basement membrane molecules (laminins, collagen IV, nidogen) which have been shown formerly to be deposited at the epithelial/mesenchymal interface in situ by the mesenchymal compartment; and (iii) soluble factors, HGF, and TGFbeta1. The three cell lines display common but also specific proliferative responses to cytokines (IL1beta, IL2, IL8, TNFalpha, IFNgamma, TGFbeta1, and HGF). When cocultured with embryonic intestinal endoderms or with human colonic Caco2 or HT29 cancer cells, C9 versus C11 and C20 cell lines induced limited versus extensive growth of the associated epithelial cells. In addition C20 cells allowed spreading of HT29 cells with the formation of a basement membrane at the heterologous interface. Morphogenesis obtained by intracoelomic grafts of associations comprising the mesenchymal cell lines and intestinal endoderms was also different among those composed of C9 cells or of C11 or C20 cells. In conclusion, these data indicate that the mucosal connective tissue is heterogeneous and comprises several phenotypically different mesenchyme-derived cells whose equilibrium may be important in the gut homeostasis. These cells can now be used to define tissue-specific factors which may be involved in the physiopathology of the intestinal epithelium.
authors.affiliation: Ontogenesis and Pathology of the Digestive Tract, INSERM Unit 381, Strasbourg, 67200, France.
authors.forename: C
authors.initials: C
authors.lastname: Fritsch
authors.forename: V
authors.initials: V
authors.lastname: Orian-Rousseaul
authors.forename: O
authors.initials: O
authors.lastname: Lefebvre
authors.forename: P
authors.initials: P
authors.lastname: Simon-Assmann
authors.forename: J M
authors.initials: JM
authors.lastname: Reimund
authors.forename: B
authors.initials: B
authors.lastname: Duclos
authors.forename: M
authors.initials: M
authors.lastname: Kedinger
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry; D018938:Caco-2 Cells; D002450:Cell Communication; D002460:Cell Line; D002642:Chick Embryo; D018920:Coculture Techniques; D003094:Collagen / Q000302:isolation & purification; D016207:Cytokines / Q000494:pharmacology*; D004386:Duodenum / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D004707:Endoderm / Q000637:transplantation; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019073:HT29 Cells; D006706:Homeostasis; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000502:physiology; D007797:Laminin / Q000302:isolation & purification; D008648:Mesoderm / Q000166:cytology* / Q000187:drug effects / Q000502:physiology / Q000637:transplantation; D009024:Morphogenesis; D051381:Rats; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
chemical_list: D016207:Cytokines; D007797:Laminin; D003094:Collagen