title: Effects of chicken thymic stromal cells on the growth and differentiation of thymocytes in vitro.
abstract: We examined contact-mediated effects of chicken thymic stromal cells (TSC) on thymocyte differentiation by co-cultivation of these cell populations. The primary cultures of TSC isolated from thymus mainly have consisted of epithelial cells which were polygonal in shape, possessed long processes and expressed MHC class II antigen. When thymocytes were co-cultured with TSC, 60% to 70% of thymocytes attached to TSC and some of them engulfed underneath TSC. These attached thymocytes were CD4-CD8- and CD4+CD8+ subsets and expressed alpha/beta TCRhigh or gamma/delta TCRlow. Some of the thymocytes attaching to TSC showed an increase of intracellular and nuclear density, fragmentation of cytoplasm and nuclei, and DNA fragmentation. And also, thymocytes attaching to TSC contained a higher percentage of cycling (S and G2 + M phase) cells than nonattaching cells. These results indicate that specific subsets in thymocytes selectively bind to TSC and undergo apoptotic death or proliferation because of interaction with TSC. Chicken TSC may play an important role in thymic differentiation by direct contact within the thymus as in mammals.
authors.affiliation: Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mukamoto
authors.forename: T
authors.initials: T
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Kodama
authors.forename: T
authors.initials: T
authors.lastname: Baba
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002645:Chickens / Q000276:immunology*; D053938:DNA Fragmentation; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D009707:Nucleosomes / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D017154:Stromal Cells / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology* / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D009707:Nucleosomes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta