title: Interorganelle signaling is a determinant of longevity in Saccharomyces cerevisiae.
abstract: Replicative capacity, which is the number of times an individual cell divides, is the measure of longevity in the yeast Saccharomyces cerevisiae. In this study, a process that involves signaling from the mitochondrion to the nucleus, called retrograde regulation, is shown to determine yeast longevity, and its induction resulted in postponed senescence. Activation of retrograde regulation, by genetic and environmental means, correlated with increased replicative capacity in four different S. cerevisiae strains. Deletion of a gene required for the retrograde response, RTG2, eliminated the increased replicative capacity. RAS2, a gene previously shown to influence longevity in yeast, interacts with retrograde regulation in setting yeast longevity. The molecular mechanism of aging elucidated here parallels the results of genetic studies of aging in nematodes and fruit flies, as well as the caloric restriction paradigm in mammals, and it underscores the importance of metabolic regulation in aging, suggesting a general applicability.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans, Louisiana 70112, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Kirchman
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Lai
authors.forename: S M
authors.initials: SM
authors.lastname: Jazwinski
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D008928:Mitochondria / Q000378:metabolism*; D015388:Organelles / Q000502:physiology*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D015398:Signal Transduction; D013997:Time Factors; D018631:ras Proteins / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D000968:Antimycin A; D018631:ras Proteins