Liquid chromatographic-electrospray tandem mass spectrometric determination of novel antifungal agents in plasma.
A rapid, selective, sensitive and reproducible HPLC-electrospray tandem mass spectrometric method has been developed for the analysis of novel triazole antifungal agents, SYN-2869 and its derivatives (SYN-2836, SYN-2903 and SYN-2921), in rat plasma using SYN-2506 as an internal standard. Isolation of these compounds from plasma and sample desalting were performed by a simple extraction procedure involving protein precipitation, vacuum-drying and reconstitution with acetonitrile. For all the agents, linearity was observed over the range of 10-10,000 ng/ml (r > or = 0.996) and the limit of quantitation was 10 ng/ml using a 100-microliter plasma volume. A measurement rate of 400-500 samples/day/instrument could be achieved using this method.
Bu
H Z
HZ
Department of Biopharmaceutics and Pharmacokinetics, SynPhar Laboratories, Inc., Edmonton, Alberta, Canada.
Khan
J K
JK
Poglod
M
M
Micetich
R G
RG
D000818:Animals; D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010879:Piperazines / Q000097:blood*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014230:Triazoles / Q000097:blood*
D000935:Antifungal Agents; D010879:Piperazines; C118964:Syn 2869; D014230:Triazoles