Effect of high hydrostatic pressure on the porin OmpC from Escherichia coli.
Porin OmpC from Escherichia coli was reconstituted in liposomes and its gating kinetics were recorded at high hydrostatic pressure, up to 90 MPa, using a development of the patch clamp technique. The composition of the recording solution influenced the results but generally high hydrostatic pressure favoured channel opening.
Macdonald
A G
AG
Department of Biomedical Sciences, University of Aberdeen, UK. a.macdonald@abdn.ac.uk
Martinac
B
B
D004926:Escherichia coli / Q000737:chemistry*; D006874:Hydrostatic Pressure; D015640:Ion Channel Gating; D007700:Kinetics; D008081:Liposomes; D018408:Patch-Clamp Techniques; D018272:Porins / Q000302:isolation & purification / Q000502:physiology*
D008081:Liposomes; C113724:OmpC protein; D018272:Porins