Effect of alpha-tocopherol pretreatment on infarct size following 90 minutes of ischemia and 4 hours of reperfusion in dogs.
The present study was designed to evaluate the effects of pretreatment with antioxidants on free radical mediated reprefusion injury. Five dogs were fed with alpha-tocopherol (1000 IU/kg, bw) for ten days prior to 90 min of left anterior descending coronary artery occlusion followed by 4 hours of reperfusion. Infarct size, serum and myocardial lipid peroxidation were measured in pretreated and in untreated animals. Infarct size, as percentage of myocardial tissue at risk in alpha-tocopherol pretreated animals was 5.86+/-0.83 and was 39.35+/-7.58 in the untreated animals. Following reprefusion in untreated group, the values of serum and myocardial tissue lipid peroxidation were significantly higher than observed values in alpha-tocopherol treated animals. alpha-tocopherol pretreatment significantly reduced the myocardial infarct size and percent necrosis in the left ventricular mass in comparison to the untreated animals. These results suggest that alpha-tocopherol pretreatment possibly helps in reducing infarct size.
Tripathi
Y
Y
Department of Physiology, Kasturba Medical College, Mangalore.
Hegde
B M
BM
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D004285:Dogs; D005609:Free Radicals / Q000097:blood; D006321:Heart / Q000187:drug effects; D015227:Lipid Peroxidation; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy*; D009206:Myocardium / Q000378:metabolism; D015427:Reperfusion Injury / Q000401:mortality / Q000517:prevention & control*; D014810:Vitamin E / Q000627:therapeutic use*
D000975:Antioxidants; D005609:Free Radicals; D014810:Vitamin E