title: Influenza C virus persistence depends on exceptional steps in viral RNA synthesis and transport.
abstract: The cell line MDCK-pi, which is persistently infected with a variant of influenza C/AnnArbor/1/50 virus (C/AA-pi), was studied as a long-term persistence model by means of a strand-specific in situ hybridization assay. As a typical feature of the persistence, we identified a continuous synthesis of antigenomic positive-strand RNA encoded by segment 7 (NS) during virus production. In contrast, infection with the parental wild-type virus led to a rapid reduction of antigenomic RNA as observed in the late period of replication particularly for RNA segment 7. Furthermore, the replication cycle of the persistent variant did not show the switch from early to late replication events followed by clearance of intracellular virus, but was regulated in terms of productive and nonproductive phases. Nonproductive phases were reversible and characterized by a low level of virus-specific RNA signals. In the productive phase, a difference in cytoplasmic RNA transport was detected for the two viruses: a marked cytoplasmic accumulation of negative- and positive-strand wild-type virus RNAs stood in contrast to a RNA localization in different cellular compartments for the persistent virus. Also, Vero cells infected with the C/AA-pi variant were restricted to a transient, non-persistent replication cycle and produced a wild-type-like course of virus-specific RNA transport. These data indicate that influenza C virus persistence depends on a distinctly modified and cell type-specific regulation of virus-specific RNA synthesis and transport.
authors.affiliation: Abteilung für Virologie, Technischen Universität München, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Zach
authors.forename: M
authors.initials: M
authors.lastname: Marschall
authors.forename: H
authors.initials: H
authors.lastname: Meier-Ewert
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004285:Dogs; D017403:In Situ Hybridization; D009982:Gammainfluenzavirus / Q000235:genetics* / Q000502:physiology; D012367:RNA, Viral / Q000096:biosynthesis* / Q000378:metabolism; D014709:Vero Cells; D017735:Virus Latency
chemical_list: D012367:RNA, Viral