Molecular mechanisms of the antiproliferative effect of vitamin K3 on Jurkat cells.
Molecular mechanisms of the antiproliferative effect of vitamin K3 (sodium 2,3-dihydro-2-methyl-1,4-naphthoquinone-2-sulfonate) were studied on a human T-lymphoma model (Jurkat cell line). It is shown that a short-term treatment of the cells with vitamin K3 damages their genomic DNA. Further culturing of the cells in the presence of vitamin K3 decreases the expression of the c-myc gene, inhibits the activity of DNA-dependent DNA-polymerases, and leads to the subsequent development of apoptosis. Combined exposure to vitamin K3 and azidothymidine has a synergistic antiproliferative effect.
Kovalenko
D V
DV
Engelhardt Institute of Molecular Biology, Russia Academy of Sciences, Moscow, 117984, Russia. azel@genome.eimb.relarn.ru.
Zelenin
A V
AV
D002455:Cell Division / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D004357:Drug Synergism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc; D006801:Humans; D019169:Jurkat Cells; D016399:Lymphoma, T-Cell / Q000473:pathology; D014812:Vitamin K / Q000494:pharmacology*; D015215:Zidovudine / Q000494:pharmacology
D014812:Vitamin K; D015215:Zidovudine