Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase.
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sensitive than the dot-ELISAs, although the difference was not statistically significant (p > 0.05). The dot-ELISA based on nitrocellulose was as sensitive as that using dacron. Stability did not differ between nitrocellulose and dacron. Specificity was lower when dacron was used than when nitrocellulose was used, although the difference was not statistically significant (p > 0.05). In conclusion, this work showed that nitrocellulose and dacron performed similarly in dot-ELISA, suggesting that they may be used alternatively in population surveillance in endemic areas.
Montenegro
S M
SM
Departamento de Imunologia, Fundação Oswaldo Cruz, Universidade Federal de Pernambuco, Recife.
da Silva
J D
JD
de Brito
M E
ME
de Carvalho Júnior
L B
LB
D000818:Animals; D000941:Antigens; D000947:Antigens, Helminth; D001700:Biomphalaria / Q000276:immunology; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D019084:Fluorescent Antibody Technique, Indirect / Q000706:statistics & numerical data; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents; D011093:Polyethylene Terephthalates; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000941:Antigens; D000947:Antigens, Helminth; D007202:Indicators and Reagents; D011093:Polyethylene Terephthalates; D003101:Collodion