Cell surface expression of the metabotropic glutamate receptor type 1alpha is regulated by the C-terminal tail.
The cell surface expression of metabotropic glutamate receptor type I splice variants has been studied using cell surface biotinylation. Co-expression of the last 86 residues of the C-terminal tail of mGluR1alpha (F2-protein) with mGluR1alpha caused a significant reduction of the amount of the cell surface receptor when compared to that in cells transfected with mGlur1alpha alone, and this was accompanied by a reduction in the production of inositol following agonist stimulation of the cells. In contrast, cell surface expression of mGluR1beta was unaltered by co-expression with the F2-protein. These results suggest that the C-terminal tail of mGluR1alpha regulates cell surface expression of the receptor.
Ciruela
F
F
Medical Research Council Anatomical Neuropharmacology Unit, Oxford, UK.
Soloviev
M M
MM
McIlhinney
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics*
D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1