Augmentation of thyroid-stimulating antibody-stimulated cyclic adenosine monophosphate response by polyethylene glycol, polyvinyl alcohol, and dextran; highly sensitive porcine thyroid cell thyroid-stimulating antibody assay.
Previously, we reported that 5% polyethylene glycol (PEG) (6000) augmented thyroid-stimulating antibody (TSAb)-stimulated cyclic adenosine monophosphate (cAMP) production in porcine thyroid cell (PTC) assay. This augmentation by PEG was specific to TSAb-stimulation. In this study we examined the effects of nonionic hydrophilic polymers such as PEG, polyvinyl alcohol (PVA), and dextran (DEX) on TSAb-stimulated cAMP production. We demonstrated that graded doses of PEG, PVA, and DEX augmented TSAb-stimulated cAMP productions; the prominent augmentations were observed with 5% PEG (20,000), 5% PEG (6000), 6% PEG (4000), 10% PVA, 14% DEX T-250, and 14% DEX T-70. PVA did not augment thyrotropin (TSH)-stimulated cAMP synthesis. Five percent PEG (20,000), 14% DEX T-250, and 14% DEX T-70 augmented TSH-stimulated cAMP synthesis very slightly. PEG, PVA, and DEX had no effects on the cAMP synthesis stimulated by GTPgammaS, forskolin, or pituitary adenylate cyclase activating polypeptide (PACAP), which stimulated adenylate cyclase. We also demonstrated that PEG, PVA, and DEX augmented the cAMP responses stimulated by small amounts (50 microL) of sera from Graves' patients; small amounts (50 microL) of sera could be used instead of purified immunoglobulin G (IgG). This may simplify the TSAb assay. We developed a highly sensitive simplified TSAb assay. PEG weakly augmented TSAb binding to isolated TSH receptor (thyrotropin-binding inhibitor immunoglobulin [TBII] increased slightly). The mechanisms of the augmentations of TSAb-stimulated cAMP productions by PEG, PVA, and DEX is not simply explained by increased binding of TSAb to the receptors. Some factors that enhance TSAb action at the receptor site are suggested.
Yamashiro
K
K
Second Department of Internal Medicine, University of Ryukyus, Nishihara, Okinawa, Japan.
Takasu
N
N
Kouki
T
T
Inui
T
T
Ochi
Y
Y
Kajita
Y
Y
Sato
Y
Y
Nagata
A
A
D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001798:Blood Proteins / Q000494:pharmacology; D002460:Cell Line; D019963:Clinical Chemistry Tests; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D003911:Dextrans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006111:Graves Disease / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D018828:Immunoglobulins, Thyroid-Stimulating / Q000097:blood* / Q000494:pharmacology*; D009479:Neuropeptides / Q000494:pharmacology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D011989:Receptors, Thyrotropin / Q000097:blood; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism
C495401:ADCYAP1 protein, human; D001323:Autoantibodies; D001798:Blood Proteins; D003911:Dextrans; D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011989:Receptors, Thyrotropin; C094372:thyrotropin-binding inhibitory immunoglobulin; D005576:Colforsin; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol; D000242:Cyclic AMP