title: Position-dependent processing of peptides presented on the surface of cowpea mosaic virus.
abstract: The plant virus cowpea mosaic virus (CPMV) has been developed as an epitope-presentation system. Numerous epitopes have been expressed in the betaB-betaC loop of the CPMV small coat protein, all of which undergo a cleavage reaction between their two carboxy-terminal residues. Although many peptides presented in this manner give an authentic immune response, this was not the case for the NIm-1A epitope from human rhinovirus-14. Crystallography revealed significant differences between the structure of NIm-1A on CPMV compared with its native configuration. The 3D structure of C PMV expressing NIm-1A was used to design alterations to the context of the NIm-1A graft.
authors.affiliation: Dept. of Virus Research, John Innes Centre, Norwich, UK.
authors.forename: K M
authors.initials: KM
authors.lastname: Taylor
authors.forename: C
authors.initials: C
authors.lastname: Porta
authors.forename: T
authors.initials: T
authors.lastname: Lin
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
authors.forename: P J
authors.initials: PJ
authors.lastname: Barker
authors.forename: G P
authors.initials: GP
authors.lastname: Lomonossoff
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D036022:Capsid Proteins; D017798:Comovirus; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000821:virology; D010455:Peptides; D011499:Protein Processing, Post-Translational; D012229:Rhinovirus / Q000737:chemistry* / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D010455:Peptides