cDNA cloning and characterization of PD1: a novel human testicular protein with different expressions in various testiculopathies.
A novel human cDNA sequence has been isolated from human testis cDNA library. This sequence, named PD1, reveals an open reading frame encoding a protein of 520 amino acids. A partial sequence similarity has been found with the RBM gene involved in the regulation of human spermatogenesis. Northern blot analysis for PD1 mRNA from several human tissues demonstrated two distinct transcripts of 2.7 (more abundant) and 4.0 kb and revealed that PD1 is expressed in testis and to a lesser extent also in spleen, thymus, and prostate. Immunohistochemical analysis of human testis showed that this protein is detected in the cytoplasm of Sertoli cells. Antibodies against a rhPD1 fragment were used for Western blot analysis, which confirmed the presence of a 60-kDa molecule in crude extract of human testicular cells obtained from fine-needle aspiration and showed different patterns in various testiculopathies, suggesting a role for such gene in human spermatogenesis.
Graziotto
R
R
Institute of Histology and Embryology, Department of Biology, Department of Biochemistry, University of Padova, Viale G. Colombo 3, Padua, 35121, Italy.
Foresta
C
C
Scannapieco
P
P
Zeilante
P
P
Russo
A
A
Negro
A
A
Salmaso
R
R
Onisto
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D016601:RNA-Binding Proteins / Q000235:genetics; D012671:Seminiferous Tubules / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012708:Sertoli Cells / Q000737:chemistry; D013076:Sperm Count; D013091:Spermatogenesis; D013737:Testis / Q000737:chemistry* / Q000473:pathology*; D014018:Tissue Distribution
D018076:DNA, Complementary; D009687:Nuclear Proteins; D011506:Proteins; C501499:RBMY1A1 protein, human; D016601:RNA-Binding Proteins; C425523:SPATA2 protein, human