Extracorporeal adsorption of endotoxin.
In a porcine endotoxin shock model using a continuous intravenous endotoxin infusion of 250 ng/kg body weight per hour, the cardiorespiratory and hematologic parameters were studied while applying a new on-line polymyxin B immobilized adsorption system. This preliminary report shows that the new adsorbent can remove endotoxin selectively from the circulation and confers a good amount of protection from endotoxin-induced cardiopulmonary decompensation as well as hematologic alterations. Survival time could be extended from 216 min to 313 min. Whereas cardiac output and mean arterial pressure declined critically after 3 h in the controls, the treated group remained stable for another 3 h. These data show that endotoxin adsorption by polymyxin B coupled covalently to acrylic spheres as an adjunctive on-line measure in the septic syndrome seems feasible.
Staubach
K H
KH
Department of Surgery, Medical University of Luebeck, Germany.
Rosenfeldt
J A
JA
Veit
O
O
Bruch
H P
HP
D000327:Adsorption; D000818:Animals; D004195:Disease Models, Animal; D004731:Endotoxins / Q000097:blood / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005260:Female; D006464:Hemoperfusion / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D010316:Particle Size; D011112:Polymyxin B; D012475:Salmonella; D012772:Shock, Septic / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D013552:Swine; D013997:Time Factors
D004731:Endotoxins; D011112:Polymyxin B