The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases.
The Rhizobium leguminosarum bv. trifolii region encoding pssA and pssB genes was cloned. The pssB gene located upstream of the pssA encoded a 28.36-kDa protein which displayed 97.5% identity with the PssB of R. leguminosarum bv. viciae. Inactivation of the pssB gene by insertion of the lacZ-Gmr cassette resulted in the significant increased production of exopolysaccharide in comparison to the wild-type level. A mutant strain was also defective in nitrogen fixation suggesting a regulatory role of pssB in symbiosis with clover.
Janczarek
M
M
Department of General Microbiology, M. Curie-Sklodowska University, Lublin, Poland.
Król
J
J
Skorupska
A
A
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D007294:Inositol; D008969:Molecular Sequence Data; D016296:Mutagenesis; D019884:Nitrogenous Group Transferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020161:Physical Chromosome Mapping; D010944:Plants / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D016961:Rhizobium leguminosarum / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013559:Symbiosis; D014158:Transcription, Genetic
D011135:Polysaccharides, Bacterial; D007294:Inositol; D019884:Nitrogenous Group Transferases; C111493:Ptdss2 protein, mouse; D010744:Phosphoric Monoester Hydrolases