Assembly of the CotSA coat protein into spores requires CotS in Bacillus subtilis.
The CotSA protein, encoded by cotSA (ytxN) of Bacillus subtilis, was detected from the cells at 5 h after the onset of sporulation (T5) and in the spore coat of wild-type cells, but not in cotE, cotS, gerE, or cotSA mutant spores. CotSA was also detected in the sporangium at T5 to T7 but not in the sporangium at T18 of cotS mutant cells, while the incorporation of CotS into the coat was not dependent upon CotSA. These results suggested that CotSA was synthesized simultaneously with CotS during T5 to T7 of sporulation and assembled into the coat dependent upon CotS.
Takamatsu
H
H
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Kodama
T
T
Watabe
K
K
D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D006602:Hexosyltransferases; D009154:Mutation; D009876:Operon; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000378:metabolism; D014157:Transcription Factors
D001426:Bacterial Proteins; C077494:CotE protein, Bacillus subtilis; C095035:CotS protein, Bacillus subtilis; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; C119243:CotSA protein, Bacillus subtilis; D006602:Hexosyltransferases