Determination of ginsenosides in plant extracts from Panax ginseng and Panax quinquefolius L. by LC/MS/MS.
An HPLC/MS/MS method has been developed for the characterization and quantification of ginsenosides contained in extracts of the root of Panax ginseng (Korean ginsengs) and Panax quinquefolius L. (American ginsengs). The [M + H]+ and [M + Na]+ ions were observed for ginsenoside standards (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1) and four different ginseng extracts. The glycosidic linkages, the core, and the attached sugar(s) of the ginsenosides can be determined from the collision-induced dissociation spectra from the protonated molecules. The relative distribution of these ginsenosides in each extract of American or Korean ginseng was established.
Wang
X
X
Perkin-Elmer Sciex Instruments, Concord, Ontario, Canada.
Sakuma
T
T
Asafu-Adjaye
E
E
Shiu
G K
GK
D002491:Central Nervous System Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D036145:Ginsenosides; D013058:Mass Spectrometry; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D012503:Saponins / Q000032:analysis*
D002491:Central Nervous System Agents; D036145:Ginsenosides; D012503:Saponins