Biochemical and genetic characteristics of TEM-29B, a novel extended spectrum beta-lactamase.
A clinical strain of Escherichia coli (strain Ec 41553) that was resistant to ceftazidime produced a TEM-type beta-lactamase with a pI of 5.4. Clavulanic acid restored the ceftazidime activity, thus suggesting an extended spectrum beta-lactamase (ESBL). The gene encoding ESBL was located in a plasmid of 57 kb. After cloning and sequencing, the ESBL (TEM-29B) showed one amino acid replacement with respect to the TEM-1 sequence, Arg-164 to His. This change increased mainly the rate of hydrolysis of ceftazidime but not of cefotaxime and aztreonam. The relevance of this substitution in the increase of ceftazidime MIC is thus stressed.
Bou
G
G
Servicio de Microbiologia, Hospital Ramón y Cajal, Madrid, Spain.
Martínez-Beltrán
J
J
Cerveró
G
G
Pérez-Díaz
J C
JC
D001398:Aztreonam / Q000378:metabolism; D002439:Cefotaxime / Q000378:metabolism; D002442:Ceftazidime / Q000378:metabolism / Q000494:pharmacology*; D018550:Cephalosporin Resistance; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D008997:Monobactams / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; D008997:Monobactams; D002442:Ceftazidime; C119242:beta-lactamase TEM-29B protein, E coli; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; D001398:Aztreonam; D002439:Cefotaxime