Mechanisms of the Eimeria tenella growth inhibitory activity induced by concanavalin A and reticuloendotheliosis virus supernatants with interferon gamma activity in chicken macrophages and fibroblasts.
Pretreatment of chicken bone marrow macrophages and embryo fibroblasts with supernatants containing chicken interferon gamma (IFN-gamma) for 24 hr prior to inoculation inhibited intracellular Eimeria tenella replication, measured by [3H] uracil incorporation. The supernatants (Sns) were obtained from culture of lymphoblastoid cells transformed by a reticuloendotheliosis virus (REV) and chicken splenocytes stimulated with concanavalin A (Con A). The mechanisms of the E. tenella growth inhibitory activity induced by Sn REV and Sn Con A in chicken macrophages and fibroblasts were studied. Addition of oxygen scavengers (superoxide dismutase, D-mannitol, DABCO, benzoic acid, L-histidine hydrochloride) was able to overcome the inhibition of E. tenella replication after pretreatment with Sn REV or Sn Con A in macrophage cultures but not in fibroblast cultures. Nitric oxide (NO) synthesis was induced in macrophage culture treated with Sn REV or Sn Con A but not in fibroblast culture. Addition of NG monomethyl-L-arginine, an NO synthase inhibitor together with the supernatants was also able to overcome inhibition of E. tenella replication in macrophage culture. On the other hand, addition of L-tryptophan to Sn REV- or Sn Con A-treated fibroblasts was able to reverse the inhibitory effect on E. tenella replication. In conclusion, production of inorganic NO or toxic oxygen intermediates may be involved in the E. tenella growth inhibitory activity of chicken macrophages pretreated with supernatants containing an IFN-gamma activity, and cellular tryptophan depletion may be involved for chicken fibroblasts, thus matching the mechanisms of the IFN-gamma-induced growth inhibitory activity for protozoans in mammals.
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CJF INSERM 93-09 Immunologie des Maladies Infectieuses-Equipe associée INRA Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, Tours, France.
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D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D019817:Benzoic Acid / Q000378:metabolism; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D003048:Coccidiosis / Q000662:veterinary*; D003208:Concanavalin A / Q000494:pharmacology*; D016786:Eimeria tenella / Q000254:growth & development*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000469:parasitology*; D006639:Histidine / Q000378:metabolism; D007371:Interferon-gamma / Q000502:physiology*; D008264:Macrophages / Q000469:parasitology*; D008353:Mannitol / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010879:Piperazines / Q000378:metabolism; D011201:Poultry Diseases / Q000469:parasitology*; D017986:Reticuloendotheliosis Viruses, Avian; D013154:Spleen / Q000187:drug effects / Q000821:virology; D013482:Superoxide Dismutase / Q000378:metabolism; D014364:Tryptophan / Q000494:pharmacology; D019323:omega-N-Methylarginine
D001120:Arginine; D019817:Benzoic Acid; D003208:Concanavalin A; D004791:Enzyme Inhibitors; D006639:Histidine; D007371:Interferon-gamma; D008353:Mannitol; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D010879:Piperazines; D013482:Superoxide Dismutase; D014364:Tryptophan; C092506:N(G)-monomethylarginine acetate; D019323:omega-N-Methylarginine; C007306:triethylenediamine