Intralobular distribution of class I alcohol dehydrogenase and aldehyde dehydrogenase 2 activities in the hamster liver.
The hepatic lobular localization of class I alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) 2 activities was examined histochemically using livers of hamsters with high ethanol preferences. The activity of class I ADH detected by the nitro blue tetrazolium method using 5 mM ethanol as a substrate was extremely high and was almost homogeneously distributed throughout the lobule. The ALDH 2 activity (substrate, 8 microM acetaldehyde) was localized to the centrilobular zone, whereas low Km ALDH (ALDH 1 + ALDH 2) activity (substrate, 50 microM acetaldehyde) showed a gradient distribution in the lobule with high centrilobular to moderate periportal activity, suggesting that the ALDH 1 activity was distributed throughout the lobule.
Watabiki
T
T
Department of Legal Medicine, Kansai Medical University, Moriguchi, Japan.
Tokiyasu
T
T
Yoshida
M
M
Okii
Y
Y
Yoshimura
S
S
Akane
A
A
D000426:Alcohol Dehydrogenase / Q000032:analysis*; D000444:Aldehyde Dehydrogenase / Q000032:analysis*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000818:Animals; D006224:Cricetinae; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D005518:Food Preferences; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology*; D014018:Tissue Distribution
D000431:Ethanol; D000426:Alcohol Dehydrogenase; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial