Anti-HIV-1 activity of an ionically modified porous polypropylene membrane determined by filtration of a viral suspension.
We describe here a unique anti-HIV-1 membrane, derived from a chemically modified porous polypropylene (PP) membrane, which lowers viral infectivity upon the filtration of HIV-1 suspension. A cationic polymer, polyethyleneimine (PEI) was graft-polymerized onto the PP filter membrane (PP-PEI), and infectious HIV-1(HTLV-IIIB) derived from MOLT-4/HIV-1(HTLV-IIIB) cells (HIV-1(HTLV-IIIB(MOLT-4)) was applied. When a viral suspension of high titer (10(3.93) TCID50 ml(-1) was filtered, efficient reduction (>99%) of gag p24 antigen levels and infectious titer resulted. In a viral suspension of medium titer (10(2.37) TCID50 ml(-1), a significant decrease in the p24 antigen did not occur, although the titer was markedly reduced (>95%). Electron microscopic observation suggested that PEI induced viral aggregations under high titer conditions, and under medium titer conditions, PEI deprived HIV-1(HTLV-IIIB(MOLT-4)) of its infectivity alone to avoid virus adsorption. In contrast, HIV-1 propagated in human peripheral blood mononuclear cells (PBMC) such as HIV-1(HTLV-III(PBMC)) was more efficiently trapped by PP-PEI at lower titers as compared with HIV-1(HTLV-IIIB(MOLT-4)) from MOLT-4/HIV-1(HTLV-IIIB) cells. These data suggest host cell modification in the interactions between PP-PEI and HIV-1 strains. Since HIV-1(HTLV-IIIB(MOLT-4)) and HIV-1(HTLV-IIIB(PBMC)) were almost electrically neutral and negative, respectively, we concluded that the divergent effect of PEI on each HIV-1(HTLV-IIIB) resulted from their different electrical characteristics.
Owada
T
T
Department of New Materials Section, Terumo Research and Development Center, Hadano, Kanagawa, Japan. JZG00166@nifty.ne.jp
Miyashita
Y
Y
Motomura
T
T
Onishi
M
M
Yamashita
S
S
Yamamoto
N
N
D002460:Cell Line; D005374:Filtration; D015497:HIV-1 / Q000648:ultrastructure; D006801:Humans; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011094:Polyethyleneimine; D011126:Polypropylenes; D014771:Virion / Q000648:ultrastructure
D008567:Membranes, Artificial; D011126:Polypropylenes; D011094:Polyethyleneimine