title: Zinc deficiency and oxidative stress in the retina of pigmented rats.
abstract: OBJECTIVE
  To determine the effect of moderate zinc deficiency on antioxidant defenses and measures of oxidative stress in the retina and retinal pigment epithelium (RPE) of Brown Norway Rats.
  
  
  METHODS
  Twenty-four rats were housed individually and divided into three groups of 8 rats each. Group 1 was fed ad libitum a semipurified control diet formulated to contain 50 parts per million [ppm] total zinc; group 2 was fed ad libitum an identical diet but containing 5 ppm total zinc; and group 3 was pair-fed the control diet but restricted in amount to that consumed by group 2. Food intake was measured daily and the rats weighed weekly. After 6 weeks, the rats were killed and the following measurements were made: serum zinc, serum alkaline phosphatase, retinal zinc, RPE-choroid zinc, RPE-choroid catalase, liver metallothionein (MT), retinal MT, RPE-choroid MT, retinal catalase, and retinal thiobarbituric reactive substances (TBARS).
  
  
  RESULTS
  The following showed statistically significant differences between groups 2 and 3, respectively: serum Zn (1216 micro/l versus 1555 microg/l, P < or = 0.01), serum alkaline phosphatase (3.75 U/mg versus 5.10 U/mg, P < or = 0.05), liver MT (4.3 microg/mg protein versus 16.7 microg/mg, P < or = 0.0001), RPE-choroid MT (1.3 microg/mg protein versus 2.2 microg/mg, P < or = 0.02), retinal MT (0.85 microg/mg protein versus 2.8 microg/mg, P < or = 0.05), and retinal TBARS (6.2 nM/mg protein versus 2.2 nM/mg, P < or = 0.05).
  
  
  CONCLUSIONS
  The results show that retinal MT and RPE MT concentrations are very sensitive to intake of dietary zinc. The increase in retinal TBARS in group 2 indicates that moderate zinc deficiency increases oxidative stress to the retina. The results also suggest that MT is protective against lipid peroxidation of retinal membranes.
authors.affiliation: Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, Louisiana 70115, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Miceli
authors.forename: D J
authors.initials: DJ
authors.lastname: Tate
authors.forename: N W
authors.initials: NW
authors.lastname: Alcock
authors.forename: D A
authors.initials: DA
authors.lastname: Newsome
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002829:Choroid / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D012160:Retina / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015032:Zinc / Q000097:blood / Q000172:deficiency*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D008668:Metallothionein; D000469:Alkaline Phosphatase; D015032:Zinc