title: Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
abstract: Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a beta-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins.
authors.affiliation: Research Center for Cardiovascular Diseases, Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas-Houston Health Science Center, 77030, USA.
authors.forename: L
authors.initials: L
authors.lastname: Gong
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Millas
authors.forename: E T
authors.initials: ET
authors.lastname: Yeh
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D003001:Cloning, Molecular; D004789:Enzyme Activation; D006801:Humans; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D000075687:NEDD8 Protein; D025842:SUMO-1 Protein; D017386:Sequence Homology, Amino Acid; D044764:Ubiquitin-Activating Enzymes; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism
chemical_list: D000075687:NEDD8 Protein; C082860:NEDD8 protein, human; D025842:SUMO-1 Protein; C470301:UBA2 protein, human; D014452:Ubiquitins; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; D044764:Ubiquitin-Activating Enzymes