Electric pulse-induced precipitation of biological macromolecules in electroporation.
We found that electric discharge through solution of biological macromolecules (DNA, RNA and proteins) causes precipitation of significant portions of these macromolecules. This precipitation is a consequence of the interaction of biological macromolecules with the metal ions solubilized from the anode plate by the electric pulse, and occurs in both absence and presence of the cells in poration medium. Precipitated fractions of macromolecules sediments at the centrifugation speed used to pellet eukaryotic cells and does not dissolve when washed with buffer. Our data indicate a complication of the direct evaluation of electroporation efficiency based on the assumption that electroporated biological macromolecules which remain associated with the cells after several washes, are successfully electroinjected into the cytoplasm of cells.
Stapulionis
R
R
Institute for Biomedical Research, Kaunas Medical University, Lithuania. romas_stapulionis@fc.vdu.lt
D000818:Animals; D002021:Buffers; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D018274:Electroporation; D007501:Iron / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism
D002021:Buffers; D008670:Metals; D011506:Proteins; D012345:RNA, Transfer, Amino Acid-Specific; D012313:RNA; D004247:DNA; D007501:Iron