title: Combining solid-phase preconcentration, capillary electrophoresis and off-line matrix-assisted laser desorption/ionization mass spectrometry: intracerebral metabolic processing of peptide E in vivo.
abstract: The in vivo metabolism of peptide E was studied in the anesthetized rat using a combination of microdialysis sampling, solid-phase preconcentration capillary electrophoresis and imaging matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The metabolic profile of peptides identified by MALDI/MS showed that the primary enzymatic activity for degradation of peptide E was due to carboxypeptidases and, to a lesser extent, aminopeptidases and some trypsin-like endopeptidases. Over 75 metabolic fragments were detected from the action of these enzymes in vivo.
authors.affiliation: Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Stoeckli
authors.forename: P E
authors.initials: PE
authors.lastname: Andren
authors.forename: R M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D004745:Enkephalins / Q000032:analysis* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D051381:Rats
chemical_list: D004745:Enkephalins; C039933:peptide E (adrenal medulla)