[Phagocytosis and killing of Staphylococcus aureus by granulocytes in mice after granulocyte-macrophage colony stimulating factor (GM-CSF) injection].
GM-CSF is a hematopoietic growth factor. In vitro it stimulates the proliferation of myeloid progenitors and formation of granulocyte and macrophage colonies. It was found that GM-CSF in vitro is also stimulated the function of mature granulocytes, but we have no information about such influence in vivo. The purpose of this investigation was the evaluation in vivo of the GM-CSF effect on phagocytosis, bactericidal activity, and lysosome enzyme activities in granulocytes. GM-CSF was injected into mice subcutaneously during 5 consecutive days in the dose of 1 microgram/kg/d. The examination of the percent of cell phagocytizing bacteria (Staphylococcus aureus), NBT test, bactericidal activity and activation of acid phosphatase, alkaline phosphatase, peroxidase and esterase was performed every day and an evident increase of the tested parameters was found. These results prove in vivo activation of granulocytes by GM-CSF.
Czygier
M
M
Zakład Diagnostyki Biochemicznej Akademii Medycznej w Białymstoku.
Mroczko
B
B
Szmitkowski
M
M
Jankowski
D
D
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004950:Esterases / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D006098:Granulocytes / Q000201:enzymology / Q000276:immunology*; D007279:Injections, Subcutaneous; D008262:Macrophage Activation / Q000502:physiology; D008297:Male; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000276:immunology*
D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D009195:Peroxidase; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase