Inhibition of peroxidase, trypsin, and alpha-chymotrypsin by platinum (II) and platinum (IV) complexes.
Effects of various complexes of platinum (II) and platinum (IV) on activities of trypsin, alpha-chymotrypsin, and peroxidase were compared. The platinum (II) complexes were found to inhibit these enzymes, though with variable efficiency. The platinum (IV) complexes at concentrations < or = 0.2 mM efficiently inhibited peroxidase but had no effect on the proteases. An enzymatic assay was developed to measure the most effective peroxidase inhibitor (cisplatin) at concentrations of 5-50 microM in the presence of fivefold excess of its isomer (transplatin).
Muginova
S V
SV
School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia.
Vil'ms
E V
EV
Shekhovtsova
T N
TN
Ivanov
V B
VB
D002384:Catalysis; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors*; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D009944:Organoplatinum Compounds; D014361:Trypsin Inhibitors; D006735:Horseradish Peroxidase; D002918:Chymotrypsin