title: Single H2Kb, H2Db and double H2KbDb knockout mice: peripheral CD8+ T cell repertoire and anti-lymphocytic choriomeningitis virus cytolytic responses.
abstract: Single H2Kb, H2Db and double H2KbDb homozygous knockout (KO) mice were generated and their peripheral CD8+ T cell repertoires compared to that of C57BL/6 (B6) mice. Limited (10-20%, H2Db), substantial (30-50%, H2Kb) and profound (90%, H2KbDb) reduction of peripheral CD8+ T cells was observed in KO mice, without Vbeta diversity alteration. Classical class Ia molecules therefore ensure most but not all of the peripheral CD8+ T cell repertoire education. As expected, H2Kb but also H2Db KO mice developed choriomeningitis following intracranial infection by lymphocytic choriomeningitis virus with the same kinetics, lethality and CD8+ cell implication as wild-type B6 mice. By contrast, H2KbDb (class Ia-Ib+) KO mice survived. Choriomeningitis of H2Db KO mice was linked to the development of a subdominant (in normal B6 mice) H2Kb-restricted cytotoxic T lymphocyte response. Mice expressing a restricted set of histocompatibility class I molecules should represent useful tools to evaluate the immunological potentials of individual MHC class I molecules.
authors.affiliation: Département SIDA-Rétrovirus, Institut Pasteur, Paris, France. perarnau@pasteur.fr
authors.forename: B
authors.initials: B
authors.lastname: Pérarnau
authors.forename: M F
authors.initials: MF
authors.lastname: Saron
authors.forename: B
authors.initials: B
authors.lastname: Reina San Martin
authors.forename: N
authors.initials: N
authors.lastname: Bervas
authors.forename: H
authors.initials: H
authors.lastname: Ong
authors.forename: M J
authors.initials: MJ
authors.lastname: Soloski
authors.forename: A G
authors.initials: AG
authors.lastname: Smith
authors.forename: J M
authors.initials: JM
authors.lastname: Ure
authors.forename: J E
authors.initials: JE
authors.lastname: Gairin
authors.forename: F A
authors.initials: FA
authors.lastname: Lemonnier
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000235:genetics / Q000502:physiology*; D064532:Histocompatibility Antigen H-2D; D006801:Humans; D008216:Lymphocytic Choriomeningitis / Q000276:immunology; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout
chemical_list: D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D064532:Histocompatibility Antigen H-2D