Distribution of cycling T lymphocytes in blood and lymphoid organs during immune responses.
Proliferation of murine T lymphocytes in blood, lymph nodes, and spleen was studied in four in vivo stimulation systems, using BrdU pulse-labeling of DNA-synthesizing cells. The T cell response to the superantigen Staphylococcus enterotoxin B (SEB) was studied in detail. Vbeta8+ T cells showed a peak of DNA synthesis 16-24 h after SEB injection, and the percentage of BrdU+ CD4 and CD8 T cells was higher in blood than in lymph nodes and spleen. DNA synthesis was preceded by massive migration of Vbeta8+ cells from blood to lymphoid organs, in which the early activation marker CD69 was first up-regulated. SEB-nonspecific Vbeta6+ cells showed minimal stimulation but, when cycling, also expressed a high level of CD69. The other systems studied were injection of the IFN-gamma inducer polyinosinic:polycytidylic acid, infection by the BM5 variants of murine leukemia virus (the causative agent of murine AIDS), and T cell expansion after transfer of normal bone marrow and lymph node cells into recombinase-activating gene-2-deficient mice. In each case, a peak of T cell proliferation was observed in blood. These data demonstrate the extensive redistribution of cycling T cells in the first few hours after activation. Kinetic studies of blood lymphocyte status appear crucial for understanding primary immune responses because cycling and redistributing T lymphocytes are enriched in the circulating compartment.
Vasseur
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F
Institut National de la Santé et de la Recherche Médicale Unité 345, Institut Necker, Centre National de la Recherche Scientifique, Hôpital Necker, Paris, France.
Le Campion
A
A
Pavlovitch
J H
JH
Pénit
C
C
D000208:Acute Disease; D019264:Adoptive Transfer; D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology; D016026:Bone Marrow Transplantation; D002453:Cell Cycle / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D004247:DNA / Q000096:biosynthesis / Q000097:blood; D004768:Enterotoxins / Q000494:pharmacology; D018984:Epitopes, T-Lymphocyte / Q000097:blood; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000276:immunology; D008198:Lymph Nodes / Q000637:transplantation; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011070:Poly I-C / Q000494:pharmacology; D012192:Retroviridae Infections / Q000097:blood / Q000276:immunology / Q000473:pathology; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D014412:Tumor Virus Infections / Q000097:blood / Q000276:immunology / Q000473:pathology
D000942:Antigens, Bacterial; D004768:Enterotoxins; D018984:Epitopes, T-Lymphocyte; D018089:Superantigens; C049463:enterotoxin E, Staphylococcal; D004247:DNA; D011070:Poly I-C