title: Isolation and mapping of a putative b subunit of human ATP synthase (ATP-BL) from human leukocytes.
abstract: From a human-leukocyte cDNA library, we cloned cDNA encoding a novel protein, which has a significant homology with the b subunit of ATP synthase (proton-transporting ATPase, F1F0-ATPase; EC3.6.1.34) derived from Anabaena sp. strain PCC 7120. The cDNA has an open reading frame of 1314 nucleotides corresponding to 438 amino acids. The coding sequence was 37.9% identical over 57 amino acid with b subunit of ATP synthase. The 34-amino-acid region of the predicted peptide sequence displays a coiled-coil motif that could form a complex with some other protein(s). We designated this novel gene as ATP-BL because of its homology to the b subunit of ATP synthase. The ATP-BL locus was mapped by fluorescence in situ hybridization (FISH) and radiation hybrid mapping to the q24 region of chromosome 16.
authors.affiliation: Department of Materials and Biosystem Engineering, Faculty of Engineering, Toyama University, Toyama City, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sugimoto
authors.forename: T
authors.initials: T
authors.lastname: Hatakeyama
authors.forename: M
authors.initials: M
authors.lastname: Isobe
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007962:Leukocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D006180:Proton-Translocating ATPases