title: Heat-shock protein-73 protects against small intestinal warm ischemia-reperfusion injury in the rat.
abstract: BACKGROUND
  The protective effects of heat-shock protein (hsp) in rat small intestinal warm ischemia-reperfusion (I/R) injury are poorly understood.
  
  
  METHODS
  Hsp-73 expression was induced in rat small intestine with use of sodium arsenite injected (6 mg/kg) through a catheter cannulated into the left common carotid artery 24 hours before ischemia (group 1). In the control group an equal volume of phosphate-buffered saline solution was injected (group 2). To block the induction of hsp-73 expression, sodium arsenate and quercetin (5 mg/kg) were injected (group 3).
  
  
  RESULTS
  The mean peak plasma levels of tumor necrosis factor-alpha and cytokine-induced neutrophil chemoattractant after reperfusion were lower in group 1 than in group 2. The tissue myeloperoxidase activity after reperfusion was lower in group 1 than in group 2. The mean peak plasma level of interleukin-10 after reperfusion was higher in group 1 than in group 2. The induction of hsp-73 expression reduced the synthesis of nitric oxide and the magnitude of the small intestinal warm I/R injury. The results in group 3 were similar to those in group 2.
  
  
  CONCLUSIONS
  Hsp-73 protects against small intestinal warm I/R injury by inhibiting the synthesis of inflammatory cytokines and the activation of neutrophils and by accelerating the synthesis of anti-inflammatory cytokines.
authors.affiliation: Department of Surgery, Sapporo Medical University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuruma
authors.forename: A
authors.initials: A
authors.lastname: Yagihashi
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Yajima
authors.forename: H
authors.initials: H
authors.lastname: Kameshima
authors.forename: J
authors.initials: J
authors.lastname: Araya
authors.forename: K
authors.initials: K
authors.lastname: Hirata
mesh_terms: D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D015153:Blotting, Western; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000097:blood; D006133:Growth Substances / Q000096:biosynthesis / Q000097:blood; D006360:Heat-Shock Proteins / Q000032:analysis / Q000502:physiology*; D006358:Hot Temperature; D036341:Intercellular Signaling Peptides and Proteins; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood; D007413:Intestinal Mucosa / Q000098:blood supply / Q000201:enzymology / Q000601:surgery; D007421:Intestine, Small / Q000098:blood supply* / Q000201:enzymology / Q000601:surgery*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis / Q000097:blood; D009195:Peroxidase / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000139:chemically induced / Q000517:prevention & control*; D017670:Sodium Compounds / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D018053:Arsenites; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D004791:Enzyme Inhibitors; D006131:Growth Inhibitors; D006133:Growth Substances; D006360:Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D017670:Sodium Compounds; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; C017947:sodium arsenite; D011794:Quercetin; D009195:Peroxidase