Expression of saliva-binding epitopes of the Porphyromonas gingivalis FimA protein on the surface of Streptococcus gordonii.
Porphyromonas gingivalis, a gram-negative oral anaerobic bacterium, has been implicated in the onset and development of periodontitis. The P. gingivalis fimbriae which mediate bacterial adherence to host oral sites and induce host inflammatory responses have been suggested as a potential antigen candidate. for vaccine development. This study was undertaken to generate Streptococcus gordonii vectors expressing the major subunit protein (FimA) of P. gingivalis fimbriae for testing as a potential live vaccine against periodontitis. We report here the expression of the C-terminal saliva-binding epitopes of P. gingivalis FimA on the surface of S. gordonii and demonstrate that domains containing free cysteine residues are poorly expressed on the surface of S. gordonii.
Sharma
A
A
School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York, 14214, USA. sharmaa@acsu.buffalo.edu
Honma
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K
Sojar
H T
HT
Hruby
D E
DE
Kuramitsu
H K
HK
Genco
R J
RJ
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000378:metabolism*; D036781:Fimbriae Proteins; D016966:Porphyromonas gingivalis / Q000235:genetics*; D012463:Saliva / Q000378:metabolism* / Q000382:microbiology; D013291:Streptococcus / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D000939:Epitopes; C063055:fimbrillin; D036781:Fimbriae Proteins