Combining solid-phase preconcentration, capillary electrophoresis and off-line matrix-assisted laser desorption/ionization mass spectrometry: intracerebral metabolic processing of peptide E in vivo.
The in vivo metabolism of peptide E was studied in the anesthetized rat using a combination of microdialysis sampling, solid-phase preconcentration capillary electrophoresis and imaging matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The metabolic profile of peptides identified by MALDI/MS showed that the primary enzymatic activity for degradation of peptide E was due to carboxypeptidases and, to a lesser extent, aminopeptidases and some trypsin-like endopeptidases. Over 75 metabolic fragments were detected from the action of these enzymes in vivo.
Zhang
H
H
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Stoeckli
M
M
Andren
P E
PE
Caprioli
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D004745:Enkephalins / Q000032:analysis* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D051381:Rats
D004745:Enkephalins; C039933:peptide E (adrenal medulla)