[Use of rapid methods in detection of enteroviruses].
Indirect immunofluorescence (IIF) and RT-PCR were used for rapid diagnosis of enterovirus infection during an outbreak of aseptic meningitis in Gomel. IgM to enterovirus were detected in 93.6% sera by IIF and in 71.4% cerebrospinal fluid specimens by RT-PCR. IIF takes only 1.5-2 h and RT-PCR 6-7 h. The methods are recommended for rapid diagnosis of enterovirus infection.
Eremin
V F
VF
Amvros'eva
T V
TV
Votiakov
V I
VI
Kvacheva
Z B
ZB
D'iakonova
O V
OV
Sharko
R M
RM
Titov
L P
LP
D000914:Antibodies, Viral / Q000032:analysis; D002555:Cerebrospinal Fluid / Q000821:virology; D004770:Enterovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000276:immunology; D004784:Environmental Monitoring; D005243:Feces / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D008587:Meningitis, Viral / Q000134:cerebrospinal fluid / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014871:Water Microbiology
D000914:Antibodies, Viral; D007075:Immunoglobulin M