Characterization of benzodiazepine receptor binding following kainic acid administration: an autoradiography study in rats.
Effects of status epilepticus and chronic temporal lobe epilepsy on benzodiazepine (BDZ) receptor binding in the rat brain were evaluated using in vitro autoradiography, 24 h and 40 days after kainic acid (KA) administration (10 mg/kg i.p). One day after KA-induced status epilepticus and in comparison to saline control group, significantly increased BDZ binding was detected in pyriform cortex (53%), whereas decreased binding was found in anterior amygdaloid nucleus (28%), fields CA1-3 of Ammon's horn (25%) and dentate gyrus (27%). Forty days after status epilepticus, significantly BDZ binding augmentation was noticed in dentate gyrus (65%) and in periaqueductal gray dorsolateral (52%), whereas decreased binding was found in medial thalamic nucleus (70%). Our data indicate different patterns of BDZ binding following KA administration, which may depend on the consequences of the status epilepticus (24 h after KA) and temporal lobe epilepsy (40 days after KA) per se.
Rocha
L
L
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico. rocha@cenids.ssa.gob.mx
Ondarza-Rovira
R
R
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005445:Flunitrazepam / Q000493:pharmacokinetics; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D014316:Tritium
D011963:Receptors, GABA-A; D014316:Tritium; D005445:Flunitrazepam; D007608:Kainic Acid