Localization of HArep-containing genes on the chromosome of Porphyromonas gingivalis W83.
We have mapped a group of virulence genes of Porphyromonas gingivalis to a single large fragment of the genome. These genes (rgpA, kgp, and hagA) all contain a consensus repeat sequence (HArep). rgpA and kgp encode cysteine proteases with Arg-X and Lys-X specificity, respectively, and hagA encodes a hemagglutinin. Genomic DNA fragments separated by pulse-field gel electrophoresis were blotted and probed in order to localize the genes to a 0.25-Mb NheI fragment of the P. gingivalis W83 genome. Further hybridization analyses with single- and double-restriction digestion allowed us to generate a physical map of the fragment and determine the precise locations of the protease and hemagglutinin genes. In addition, we found an insertion-like sequence, IS195, near the ends of the 0. 25-Mb NheI fragment. A similarly sized fragment carrying HArep sequences was also demonstrated in the P. gingivalis W12 and W50 genomes.
Lewis
J P
JP
Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23298-0566, USA.
Macrina
F L
FL
D001426:Bacterial Proteins; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D016384:Consensus Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D037102:Lectins; D020161:Physical Chromosome Mapping; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers; D006388:Hemagglutinins; D037102:Lectins; C114923:hemagglutinin A, Porphyromonas gingivalis; D003546:Cysteine Endopeptidases