Transformation of bile acids and sterols by clostridia (fusiform bacteria) in Wistar rats.
The effects on bile acid and sterol transformation of clostridia (fusiform bacteria), the dominant intestinal bacteria in rodents (ca. 10(10) counts per g wet feces) were examined in Wistar rats. After inoculation of clostridia into germ-free rats and into rats previously inoculated solely with Escherichia coli, most of the endogenous bile acids were deconjugated, and cholic acid and chenodeoxycholic acid were 7alpha-dehydroxylated to deoxycholic acid and lithocholic acid, respectively. Tauro-beta-muricholic acid, another major bile acid in rats, was deconjugated, but only part of it (ca. 30%) was transformed into hyodeoxycholic acid. Cholesterol and sitosterol were also reduced to coprostanol and sitostanol, respectively. Escherichia coli transformed neither bile acids nor sterols. These data suggest that clostridia play an important role in the formation of secondary bile acids and coprostanol in rats.
Uchida
K
K
Strategic Information Unit, Shionogi & Co., Ltd., Shibuya, Tokyo, Japan. kiyohisha.uchida@shionogi.co.jp
Satoh
T
T
Narushima
S
S
Itoh
K
K
Takase
H
H
Kuruma
K
K
Nakao
H
H
Yamaga
N
N
Yamada
K
K
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis / Q000378:metabolism*; D004083:Cholestanol / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D019826:Cholic Acid / Q000032:analysis; D003013:Clostridium / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005260:Female; D005856:Germ-Free Life; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012855:Sitosterols / Q000378:metabolism; D013261:Sterols / Q000378:metabolism*; D013656:Taurocholic Acid / Q000031:analogs & derivatives / Q000378:metabolism
D001647:Bile Acids and Salts; D012855:Sitosterols; D013261:Sterols; C037351:tauromuricholic acid; D013656:Taurocholic Acid; C025473:gamma-sitosterol; D004083:Cholestanol; D002784:Cholesterol; D019826:Cholic Acid