SPR analysis of the total reduction of protein adsorption to surfaces coated with mixtures of long- and short-chain polyethylene oxide block copolymers.
PEO/PPO/PEO triblock copolymers have previously been shown to reduce the binding of proteins to a variety of surfaces. In this study, mixtures of long- and short-chain copolymers have been shown to adhere to gold substrate surface plasmon resonance slides. The mixtures have been shown to significantly reduce the binding of BSA to gold surfaces, compared to the more commonly used long chain PEO copolymers. These mixtures have been shown to be more effective, than either short, or long-chain copolymers used individually, complementing a published theoretical treatise of PEO surfactant behaviour towards protein interaction with surfaces.
Pavey
K D
KD
Novel Methods Group, CASS, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK. karl_d_pavey@sbphrd.com
Olliff
C J
CJ
D000327:Adsorption; D020099:Coated Materials, Biocompatible; D006046:Gold; D007700:Kinetics; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods
D020099:Coated Materials, Biocompatible; C116176:PEO-PPO-PEO; D011409:Propylene Glycols; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006046:Gold