title: Nucleus-encoded precursors to thylakoid lumen proteins of Euglena gracilis possess tripartite presequences.
abstract: The complete presequences of the nucleus-encoded precursors to two proteins, cytochrome c6 and the 30-kDa protein of the oxygen-evolving complex, that reside in the thylakoid lumen of the chloroplasts of Euglena gracilis are presented. Sorting of these proteins involves translocation across four membranes, the three-membraned chloroplast envelope and the thylakoid membrane. The tripartite presequences show the structure: signal sequence transit sequence signal sequence. Three hydrophobic domains become apparent: two of them correspond to signal sequences for translocation across the endoplasmic reticulum (ER) membrane and the thylakoid membrane, respectively, whereas the third constitutes the stop-transfer signal contained in the long stroma-targeting part of the tripartite presequence.
authors.affiliation: Institute of Molecular and Subcellular Biology, Comenius University, Bratislava, Slovak Republic.
authors.forename: R
authors.initials: R
authors.lastname: Vacula
authors.forename: J M
authors.initials: JM
authors.lastname: Steiner
authors.forename: J
authors.initials: J
authors.lastname: Krajcovic
authors.forename: L
authors.initials: L
authors.lastname: Ebringer
authors.forename: W
authors.initials: W
authors.lastname: Löffelhardt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics*; D003580:Cytochromes / Q000235:genetics*; D045348:Cytochromes f; D005056:Euglena gracilis / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid
chemical_list: D003580:Cytochromes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; C064388:oxygen-evolving complex, 33 kDa protein; D045348:Cytochromes f