title: Carboxy-terminal processing of the large subunit of [Fe] hydrogenase from Desulfovibrio desulfuricans ATCC 7757.
abstract: hydA and hydB, the genes encoding the large (46-kDa) and small (13. 5-kDa) subunits of the periplasmic [Fe] hydrogenase from Desulfovibrio desulfuricans ATCC 7757, have been cloned and sequenced. The deduced amino acid sequence of the genes product showed complete identity to the sequence of the well-characterized [Fe] hydrogenase from the closely related species Desulfovibrio vulgaris Hildenborough (G. Voordouw and S. Brenner, Eur. J. Biochem. 148:515-520, 1985). The data show that in addition to the well-known signal peptide preceding the NH2 terminus of the mature small subunit, the large subunit undergoes a carboxy-terminal processing involving the cleavage of a peptide of 24 residues, in agreement with the recently reported data on the three-dimensional structure of the enzyme (Y. Nicolet, C. Piras, P. Legrand, E. C. Hatchikian, and J. C. Fontecilla-Camps, Structure 7:13-23, 1999). We suggest that this C-terminal processing is involved in the export of the protein to the periplasm.
authors.affiliation: Unité de Bioénergétique et Ingéniérie des Protéines, IBSM, CNRS, 13402 Marseilles Cedex 20, France. hatch@ibsm.cnrs-mrs.fr
authors.forename: E C
authors.initials: EC
authors.lastname: Hatchikian
authors.forename: V
authors.initials: V
authors.lastname: Magro
authors.forename: N
authors.initials: N
authors.lastname: Forget
authors.forename: Y
authors.initials: Y
authors.lastname: Nicolet
authors.forename: J C
authors.initials: JC
authors.lastname: Fontecilla-Camps
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D003001:Cloning, Molecular; D003901:Desulfovibrio / Q000201:enzymology* / Q000235:genetics; D016969:Desulfovibrio vulgaris / Q000201:enzymology / Q000235:genetics; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D019897:Periplasm / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D008565:Membrane Proteins; C118830:iron hydrogenase; D006864:Hydrogenase