title: Biosynthesis of phytochelatins in the fission yeast. Phytochelatin synthesis: a second role for the glutathione synthetase gene of Schizosaccharomyces pombe.
abstract: By complementation screening of a cadmium-sensitive Schizosaccharomyces pombe mutant deficient in phytochelatin synthesis, but with 44% of the wild-type glutathione content, we cloned a DNA fragment involved in phytochelatin synthesis. Sequence analysis revealed that it encodes the second enzyme involved in glutathione (GSH) biosynthesis, glutathione synthetase (GSH2) (E.C.6.3.2.3, Wang and Oliver, 1997). The mutant allele shows a single base-pair exchange at the 3' end of the reading frame leading to a single amino acid change from glycine to aspartate. This mutation leads to a significant reduction of phytochelatin synthesis, whereas glutathione synthesis is impaired to a far lesser extent. Complementation with the Arabidopsis thaliana GSH2 cDNA led to a partial restoration of phytochelatin synthesis. These data strongly suggest that the GSH2 gene encodes a bifunctional enzyme that is able to catalyse both the synthesis of GSH by adding glycine to the dipeptide (gammaGlu-Cys) and the synthesis of phytochelatins. The sequence has been submitted to EMBL, Accession No. Y08414.
authors.affiliation: Institut für Biologie IV (Mikrobiologie), Rheinisch-Westfälische Technische Hochschule Aachen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Al-Lahham
authors.forename: V
authors.initials: V
authors.lastname: Rohde
authors.forename: P
authors.initials: P
authors.lastname: Heim
authors.forename: R
authors.initials: R
authors.lastname: Leuchter
authors.forename: J
authors.initials: J
authors.lastname: Veeck
authors.forename: C
authors.initials: C
authors.lastname: Wunderlich
authors.forename: K
authors.initials: K
authors.lastname: Wolf
authors.forename: M
authors.initials: M
authors.lastname: Zimmermann
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology; D004271:DNA, Fungal / Q000235:genetics; D005816:Genetic Complementation Test; D005978:Glutathione / Q000096:biosynthesis; D005981:Glutathione Synthase / Q000235:genetics* / Q000378:metabolism; D008667:Metalloproteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D054811:Phytochelatins; D010940:Plant Proteins / Q000096:biosynthesis*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D008667:Metalloproteins; D010940:Plant Proteins; D002104:Cadmium; D054811:Phytochelatins; D005981:Glutathione Synthase; D005978:Glutathione