title: Cellular and humoral immune responses to MUC1 mucin and tandem-repeat peptides in ovarian cancer patients and controls.
abstract: The objective of this study was to demonstrate the presence of proliferative T cell responses to human polymorphic epithelial mucin (MUC1) and its tandem-repeat peptides in peripheral blood mononuclear cells (PBMC) from ovarian cancer patients and from controls and to correlate these cellular responses to a humoral response to MUC1. PBMC were obtained from 6 healthy women, from 13 women in the third trimester of pregnancy and from 21 ovarian cancer patients. Only 1 of the 6 healthy women showed a weak primary proliferative response (stimulation index, SI <2) to a 20-mer MUC1 tandem-repeat peptide in the presence of interleukin-2 (IL-2). In PBMC from 5/13 pregnant women (38%) a weak response could be induced by the 20-mer and/or 60-mer tandem-repeat peptides (SI < or =3.0) and in PBMC from 8/15 ovarian cancer patients (53%) 20-mer and/or 60-mer MUC1 tandem-repeat peptides induced primary responses (SI < or =5.4). MUC1 mucin purified from a breast tumor cell line and/or from urine of a healthy donor had a relatively strong stimulating effect (SI < or =19) on PBMC from 4 of 16 ovarian cancer patients (25%). In contrast, in PBMC of 9 ovarian cancer patients stimulated by the addition of a Candida albicans extract, MUC1 mucin strongly inhibited proliferation. This inhibition could partially be abrogated by the addition of IL-2. MUC1 (CA 15.3 assay) and free circulating MUC1 IgG and IgM antibodies (PEM.CIg assay) were determined in the plasma of all subjects. The MUC1 and the free circulating MUC1 IgG antibody plasma levels were significantly higher in the ovarian cancer patients than in the healthy women. Although no significant correlations were found between MUC1 mucin, MUC1 Ab plasma levels and the individual proliferative responses to the MUC1 antigens, an association may exist between them, since all three are significantly higher in the ovarian cancer patients than in the healthy women.
authors.affiliation: Department of Obstetrics and Gynaecology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands. snijdewint@azvu.nl
authors.forename: F G
authors.initials: FG
authors.lastname: Snijdewint
authors.forename: S
authors.initials: S
authors.lastname: von Mensdorff-Pouilly
authors.forename: A H
authors.initials: AH
authors.lastname: Karuntu-Wanamarta
authors.forename: A A
authors.initials: AA
authors.lastname: Verstraeten
authors.forename: I
authors.initials: I
authors.lastname: van Zanten-Przybysz
authors.forename: P
authors.initials: P
authors.lastname: Hummel
authors.forename: H W
authors.initials: HW
authors.lastname: Nijman
authors.forename: P
authors.initials: P
authors.lastname: Kenemans
authors.forename: J
authors.initials: J
authors.lastname: Hilgers
mesh_terms: D000595:Amino Acid Sequence; D000917:Antibody Formation / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000097:blood / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology*; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology; D020080:Tandem Repeat Sequences
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018396:Mucin-1