Cell division tracking and expansion of hematopoietic long-term repopulating cells.
The combined use of rigorous assays for quantitating transplantable stem cell numbers and precise cell labeling and tracking procedures have provided definitive evidence that stem cell self-renewal divisions can occur in vitro in the absence of stromal feeder layers. These findings set the stage for defining conditions that may alter the ability of these cells to maintain their primitive status when mitogenically activated.
Oostendorp
R A
RA
Audet
J
J
Miller
C
C
Eaves
C J
CJ
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006085:Graft Survival; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D051379:Mice; D009930:Organic Chemicals; D010641:Phenotype; D017154:Stromal Cells / Q000166:cytology; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D016298:Hematopoietic Cell Growth Factors; D007378:Interleukins; D009930:Organic Chemicals; C070080:PKH 26; C068241:PKH-2; D013388:Succinimides