The reduction state of the Q-pool regulates the electron flux through the branched respiratory network of Paracoccus denitrificans.
In this work we demonstrate how the reduction state of the Q-pool determines the distribution of electron flow over the two quinol-oxidising branches in Paracoccus denitrificans: one to quinol oxidase, the other via the cytochrome bc1 complex to the cytochrome c oxidases. The dependence of the electron-flow rate to oxygen on the fraction of quinol in the Q-pool was determined in membrane fractions and in intact cells of the wild-type strain, a bc1-negative mutant and a quinol oxidase-negative mutant. Membrane fractions of the bc1-negative mutant consumed oxygen at significant rates only at much higher extents of Q reduction than did the wild-type strain or the quinol oxidase-negative mutant. In the membrane fractions, dependence on the Q redox state was exceptionally strong corresponding to elasticity coefficients close to 2 or higher. In intact cells, the dependence was weaker. In uncoupled cells the dependence of the oxygen-consumption rates on the fractions of quinol in the Q-pool in the wild-type strain and in the two mutants came closer to that found for the membrane fractions. We also determined the dependence for membrane fractions of the wild-type in the absence and presence of antimycin A, an inhibitor of the bc1 complex. The dependence in the presence of antimycin A resembled that of the bc1-negative mutant. These results indicate that electron-flow distribution between the two quinol-oxidising branches in P. denitrificans is not only determined by regulated gene expression but also, and to a larger extent, by the reduction state of the Q-pool.
Otten
M F
MF
Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Free University, The Netherlands.
Reijnders
W N
WN
Bedaux
J J
JJ
Westerhoff
H V
HV
Krab
K
K
Van Spanning
R J
RJ
D004579:Electron Transport / Q000187:drug effects; D007700:Kinetics; D008564:Membrane Potentials; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000378:metabolism* / Q000502:physiology; D011809:Quinones / Q000378:metabolism
D011809:Quinones; D010100:Oxygen