title: Utility of liquid-based cytology for cervical carcinoma screening: results of a population-based study conducted in a region of Costa Rica with a high incidence of cervical carcinoma.
abstract: BACKGROUND
  In a study using a split-sample design, liquid-based cytology (ThinPrep Processor, Cytyc Corporation, Boxborough, MA) was compared with the conventional Papanicolaou (Pap) smear in Guanacaste, Costa Rica. The study provides the first population-based comparison of the ThinPrep screening technology and includes "gold standard" measures of diagnostic accuracy.
  
  
  METHODS
  The population-based study was performed among over 8000 women residing in a Costa Rican province with a high incidence of cervical carcinoma. Conventional smears were prepared and diagnosed in Costa Rica, while the residual material on the sampling device was collected into a liquid preservative and shipped to the U.S., where ThinPrep cytologic slides were prepared and diagnosed. Cytologic diagnoses based on the two techniques, categorized according to the Bethesda System, were compared with a "gold standard" final case diagnosis for each patient, also based on Bethesda terminology, that reflected an integrated interpretation of all available data, including cytology, histology, and cervicography. Results were also compared with the results of HPV DNA detection (Hybrid Capture, Digene Corporation, Silver Spring, MD).
  
  
  RESULTS
  ASCUS was the threshold for colposcopy referral. There were significantly more women referred according to this threshold with the ThinPrep slide (12.7%) than with the conventional smear (6.7%, P<0.001). Compared with the final case diagnosis, referral by ThinPrep slides detected 92.9% of cases with high grade squamous intraepithelial lesions (HSIL) and 100% of carcinoma cases. Smears detected 77.8% of HSIL and 90.9% of carcinomas. Thus, ThinPrep cytology was significantly more sensitive in the detection of HSIL and cancer (McNemar test, P<0.001). Adjudication of cases in which the ThinPrep and smear diagnoses disagreed, using the final case diagnoses and the HPV DNA test results as reference standards, suggested that the ThinPrep method was detecting additional true SIL as opposed to false-positives.
  
  
  CONCLUSIONS
  In a population-based study of high risk women, ThinPrep cytology demonstrated significantly increased sensitivity for detecting HSIL and carcinoma, with a concurrent significant increase in colposcopy referrals.
authors.affiliation: Women & Infants Hospital, Brown University, Providence, Rhode Island 02905-2401, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Hutchinson
authors.forename: D J
authors.initials: DJ
authors.lastname: Zahniser
authors.forename: M E
authors.initials: ME
authors.lastname: Sherman
authors.forename: R
authors.initials: R
authors.lastname: Herrero
authors.forename: M
authors.initials: M
authors.lastname: Alfaro
authors.forename: M C
authors.initials: MC
authors.lastname: Bratti
authors.forename: A
authors.initials: A
authors.lastname: Hildesheim
authors.forename: A T
authors.initials: AT
authors.lastname: Lorincz
authors.forename: M D
authors.initials: MD
authors.lastname: Greenberg
authors.forename: J
authors.initials: J
authors.lastname: Morales
authors.forename: M
authors.initials: M
authors.lastname: Schiffman
mesh_terms: D002584:Cervix Uteri / Q000473:pathology; D015331:Cohort Studies; D003364:Costa Rica / Q000453:epidemiology; D003581:Cytodiagnosis / Q000295:instrumentation / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D015994:Incidence; D008403:Mass Screening; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis; D014412:Tumor Virus Infections / Q000175:diagnosis; D002578:Uterine Cervical Dysplasia / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology* / Q000821:virology; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral