Simple method for DNA extraction from pancreatic juice for PCR amplification assays.
CONCLUSIONS
Preparation of DNA from pancreatic juice for subsequent polymerase chain reaction (PCR) is difficult, but manageable. The protocol presented offers a simple and fast solution. This method might be applicable to other complicated samples, such as saliva, would secretions, or stool washings.


BACKGROUND
Of all the biological samples used for PCR amplification, pancreatic juice is the most problematic because of the presence of potential inhibitory substances and the amount of nucleases. This demands a DNA preparation procedure that is suitable for routine diagnostic PCR, and is therefore efficient and safe. This is particularly true for pancreatic juice obtained during routine endoscopy.


METHODS
We describe here a simple method utilizing modified phenol/chloroform extraction and precipitation directly from native pancreatic juice suitable for diagnostic PCR applications, such as oncogenes.


RESULTS
DNA could be prepared in quantitative amounts from routine endoscopic specimens. DNA could also be prepared from samples kept several days at room temperature.
Müller
P
P
Department of Medicine, University of Rostock, Germany.
Jesnowski
R
R
Liebe
S
S
Rolfs
A
A
Löhr
M
M
D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D011905:Genes, ras; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D010189:Pancreatic Juice / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA