title: The effect of mitomycin-C on the healing process in rabbit extraocular muscle surgery.
abstract: OBJECTIVE
  One of the most serious complications following extraocular muscle surgery is formation of postoperative adhesions that may result in restrictive ocular motility dysfunction. The effect of mitomycin-C (MMC) on postoperative wound healing in extraocular muscle surgery was investigated.
  
  
  METHODS
  Sixteen albino rabbits (24 eyes) were used and anesthetized with ketamine and xylazine. These rabbits were divided into 3 groups; (1) control group (balanced salt solution (BSS) exposure for 5 minutes), (2) Group 1 (0.5 mg/ml MMC exposure for 1 minute) and (3) Group 2 (0.5 mg/ml MMC exposure for 5 minutes). After 3 mm recession of superior rectus muscle, MMC or BSS was applied using Weck-Cel sponge (Solan, Jacksonville, FL). The reduction of fibroblast and collagen fiber proliferation were evaluated histopathologically with light microscope at 1, 2, 4 and 8 weeks after operation. We also evaluated the amount of basic fibroblast growth factor (bFGF) by immunohistochemical study at the same time.
  
  
  RESULTS
  Histopathologically, there was a more significant reduction of fibroblast and collagen fiber proliferation in Group 1 and 2 than the control group. Immunohistochemically, Group 1 and 2 showed lesser expression of bFGF than the control group. But, there was no difference between Group 1 and 2.
  
  
  CONCLUSIONS
  Topical intraoperative application of mitomycin C in extraocular muscle surgery may reduce postoperative adhesions.
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Oh
authors.forename: D W
authors.initials: DW
authors.lastname: Park
authors.forename: B L
authors.initials: BL
authors.lastname: Chang
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005500:Follow-Up Studies; D016685:Mitomycin / Q000494:pharmacology*; D015835:Ocular Motility Disorders / Q000601:surgery; D009801:Oculomotor Muscles / Q000601:surgery*; D013508:Ophthalmologic Surgical Procedures; D011817:Rabbits; D012590:Sclera / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000267:Tissue Adhesions / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016222:Fibroblast Growth Factor 2; D016685:Mitomycin