title: Determination of clindamycin in human plasma by high-performance liquid chromatography using coupled columns.
abstract: A rapid automated method has been developed for the determination of clindamycin, a lincosamide antibiotic, in human plasma. Coupled column HPLC was used after precipitation of plasma proteins with a saturated ammonium sulfate solution. As a first step, the drug and internal standard were trapped on a precolumn of LiChrospher 60RP-select B. A reversed-phase Nucleosil 100 C18 HD column then separated drug and internal standard from each other and from remaining plasma components. The assay was validated in the range 0.2-10.0 micrograms ml-1 plasma. The results obtained for accuracy, intra- and inter-day precision complied very well with the generally accepted criteria for bioanalytical assays.
authors.affiliation: Zentrallaboratorium Deutscher Apotheker, Eschborn, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Fieger-Büschges
authors.forename: G
authors.initials: G
authors.lastname: Schüssler
authors.forename: V
authors.initials: V
authors.lastname: Larsimont
authors.forename: H
authors.initials: H
authors.lastname: Blume
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002981:Clindamycin / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin