title: Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition.
abstract: RNA editing in higher plant plastids alters mRNA sequences by C-to-U conversions at highly specific sites through an unknown mechanism. To elucidate how the cytidine residues to be edited are specifically recognized and distinguished from other cytidines in close proximity, we have changed in vivo the distances of two plastid RNA-editing sites from their essential upstream cis-acting sequence element. Analysis of RNA editing in transgenic chloroplasts revealed that reduction of this distance by 1 nt entirely abolishes RNA editing. Surprisingly, deletions or combinations of deletional and point mutations that shift a heterologous cytidine residue in the same distance from the upstream cis-element as the editing site in the wild type result in transfer of the RNA-editing activity to the heterologous cytidine whereas the wild-type site remains unedited. Our results suggest that the molecular identity of at least some editing sites in the chloroplast genome is defined by their distance from an essential upstream sequence element.
authors.affiliation: Institut fur Biologie III, Universitat Freiburg, Schanzlestrasse 1, D-79104 Freiburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hermann
authors.forename: R
authors.initials: R
authors.lastname: Bock