High oxygen radical production is associated with fast parasite clearance in children with Plasmodium falciparum malaria.
It has been hypothesized that reactive oxygen intermediates (ROI) released by leukocytes play a major role in the immune response to many infectious agents. In the present study, the parasitologic and clinical courses of 75 Gabonese children with Plasmodium falciparum malaria were compared with the ability of their granulocytes to produce oxygen radicals. The luminol-dependent chemiluminescence in granulocyte suspensions for the children was measured without stimulation and after stimulation with phorbol-12-myristate-13-acetate, N-formyl-methionyl-leucyl-phenylalanine, or tumor necrosis factor. A significant association was found between fast parasite clearance time and high oxygen radical generation in both the unstimulated and stimulated granulocyte preparations. No correlation was found between fever clearance time and ROI generation. These findings suggest that ROI play a pivotal role in the immune response as a first line of defense against P. falciparum malaria.
Greve
B
B
Sektion Humanparasitologie, Institut für Tropenmedizin, Universität Tübingen, D-72074 Tübingen, Germany.
Lehman
L G
LG
Lell
B
B
Luckner
D
D
Schmidt-Ott
R
R
Kremsner
P G
PG
D002648:Child; D002675:Child, Preschool; D005260:Female; D005609:Free Radicals; D005681:Gabon; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000276:immunology*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D026082:Singlet Oxygen; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D005609:Free Radicals; D014409:Tumor Necrosis Factor-alpha; D026082:Singlet Oxygen; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen