Interferon-gamma and lipopolysaccharide induce mouse guanylate-binding protein 3 (mGBP3) expression in the murine macrophage cell line RAW264.7.
Mouse guanylate-binding protein 3 (mGBP3) is a 71-kDa GTPase which belongs to GTP-binding protein family. The present study showed that the expression of mGBP3 transcript was readily induced in a dose dependent fashion in the macrophage cell line RAW264.7 treated with either interferon-gamma (IFN-gamma) or lipopolysaccharide (LPS). The expression of mGBP3 protein was also apparent by 4 and 6 h after the treatment of cells with IFN-gamma (100 U/ml) or LPS (1 microgram/ml), and remained at plateau for at least 24 h. Cycloheximide (10 micrograms/ml) had no effect on the IFN-gamma- or LPS-induced mGBP3 expression, suggesting that the mGBP3 induction did not require further protein synthesis. Interestingly, a protein kinase C (PKC) inhibitor staurosporine (50 nM) abolished the induction of mGBP3 expression by LPS, but not by IFN-gamma. These findings suggest that mGBP3 may be involved in the macrophage activation process and both IFN-gamma and LPS induce the mGBP3 expression through distinct signal transduction pathways.
Han
B H
BH
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
D000818:Animals; D002460:Cell Line; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D011493:Protein Kinase C / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D019311:Staurosporine / Q000494:pharmacology
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; D019311:Staurosporine