Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area.
Two beta-lactamase gene regions were characterized by DNA sequencing in eight clinical isolates of Klebsiella oxytoca. The blaOXY-2a region encoded a beta-lactamase nearly identical to OXY-2 (one amino acid residue substituted) and conferred aztreonam and cefuroxime resistance on the K. oxytoca isolates. Overproduction of OXY-2a was caused by a G-to-A substitution of the fifth nucleotide in the -10 consensus sequence of blaOXY-2a. The blaOXY-1a was identified in a susceptible strain, and the OXY-1a enzyme differed from OXY-1 by two amino acid residues.
Wu
S W
SW
The Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institute and Karolinska Hospital, 171 76 Stockholm, Sweden. wus@rockvax.rockefeller.edu
Dornbusch
K
K
Kronvall
G
G
D000595:Amino Acid Sequence; D016470:Bacteremia / Q000188:drug therapy / Q000382:microbiology*; D001483:Base Sequence; D005798:Genes, Bacterial; D006801:Humans; D007709:Klebsiella / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology*; D008969:Molecular Sequence Data; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases