title: Quantitative analysis of histone H1 degrees protein synthesis in HTC cells.
abstract: H1 degrees, a member of histone H1 family associated with cell growth arrest and differentiation, is barely expressed in most mammalian cells in culture. Depending on the cell type, serum deprivation or drugs, such as sodium butyrate, significantly increase H1 degrees mRNA level and H1 degrees protein accumulates. However, probably because of a lack of a simple quantitative procedure, little is known about the relationship between H1 degrees mRNA content and its effective translation rate. Using a rat hepatoma cell line and sodium butyrate as a model system, we attempted to evaluate this in different cellular conditions by measuring H1 degrees synthesis with a rapid quantitative procedure we described previously. We found that although the amount of H1 degrees mRNA rapidly increased and then stabilized under sodium butyrate treatment, its transcription was delayed and H1 degrees protein was synthesized in a progressive wave. Butyrate removal from cell culture confirmed that mRNA level and protein synthesis were independently regulated, and provided evidence that sodium butyrate would not directly target the translation apparatus. In contrast, during the S phase of the cell cycle, H1 degrees gene transcription and protein synthesis were concomitantly activated. Taken together these data provide evidence that H1 degrees accumulation results from an increase of its synthesis and that, depending on conditions, a cell exhibits a H1 degrees translation efficiency which may or may not reflect the mRNA level.
authors.affiliation: Laboratoire de Biologie Moléculaire des Cellules Eucaryotes, ICGM-EA 1501, Université Paris V, Port-Royal, France.
authors.forename: L
authors.initials: L
authors.lastname: Cuisset
authors.forename: L
authors.initials: L
authors.lastname: Tichonicky
authors.forename: M
authors.initials: M
authors.lastname: Delpech
mesh_terms: D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology; D002453:Cell Cycle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D006657:Histones; D012333:RNA, Messenger; D020148:Butyric Acid