title: Reaction mechanism of phosphoglucosamine mutase from Escherichia coli.
abstract: The phosphoglucosamine mutase (GlmM) from Escherichia coli, specifically required for the interconversion of glucosamine-6-phosphate and glucosamine-1-phosphate (an essential step in the pathway for cell-wall peptidoglycan and lipopolysaccharide biosyntheses) was purified to homogeneity and its kinetic properties were investigated. The enzyme was active in a phosphorylated form and catalysed its reaction according to a classical ping-pong bi-bi mechanism. The dephosphorylated and phosphorylated forms of GlmM could be separated by HPLC and coupled MS showed that only one phosphate was covalently linked to the active site of the enzyme. The site of phosphorylation was clearly identified as Ser102 in the 445-amino acid polypeptide. GlmM was also capable of catalysing the interconversion of glucose-1-phosphate and glucose-6-phosphate isomers, although at a much lower (1400-fold) rate. Interestingly, the mutational change of the Ser100 to a threonine residue resulted in a 20-fold increase of the nonspecific phosphoglucomutase activity of GlmM, suggesting that the presence of either a serine or a threonine at this position in the consensus sequence of hexosephosphate mutases could be one of the factors that determines the specificity of these enzymes for either sugar-phosphate or amino sugar-phosphate substrates.
authors.affiliation: Hoechst-Marion-Roussel, Romainville, France.
authors.forename: L
authors.initials: L
authors.lastname: Jolly
authors.forename: P
authors.initials: P
authors.lastname: Ferrari
authors.forename: D
authors.initials: D
authors.lastname: Blanot
authors.forename: J
authors.initials: J
authors.lastname: Van Heijenoort
authors.forename: F
authors.initials: F
authors.lastname: Fassy
authors.forename: D
authors.initials: D
authors.lastname: Mengin-Lecreulx
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010733:Phosphoglucomutase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; C097631:phosphoglucosamine mutase; D010733:Phosphoglucomutase