title: Arabidopsis thaliana RNA polymerase II subunits related to yeast and human RPB5.
abstract: Arabidopsis thaliana contains at least four genes that are predicted to encode polypeptides related to the RPB5 subunit found in yeast and human RNA polymerase II. This subunit has been shown to be the largest subunit common to yeast RNA polymerases I, II, and III (RPABC27). More than one of these genes is expressed in Arabidopsis suspension culture cells, but only one of the encoded polypeptides is found in purified RNA polymerases II and III. This polypeptide has a predicted pI of 9.6, matches 14 of 16 amino acids in the amino terminus of cauliflower RPB5 that was microsequenced, and shows 42 and 53% amino acid sequence identity with the yeast and human RPB5 subunits, respectively.
authors.affiliation: Department of Biochemistry, 117 Schweitzer Hall, University of Missouri, Columbia, MO 65211, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Larkin
authors.forename: G
authors.initials: G
authors.lastname: Hagen
authors.forename: T J
authors.initials: TJ
authors.lastname: Guilfoyle
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000737:chemistry* / Q000235:genetics; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D015342:DNA Probes; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D012097:Repressor Proteins; C117184:URI1 protein, human; D012319:RNA Polymerase II