[Analysis of two cases of valproic acid intoxication by 1H NMR spectroscopy and gas chromatography/mass spectrometry].
Analysis of urine samples from two poisoned patients was performed using proton nuclear magnetic resonance (1H NMR) spectroscopy and a classical GC/MS protocol. Valproic acid as its glucuronide was identified and quantified in the 1H NMR spectra recorded directly from 0.5 mL of the collected samples. Moreover, a significant lactic aciduria could be observed. Comparison with the GC/MS findings suggests that 1H NMR spectroscopy can be clinically useful.
Azaroual
N
N
Laboratoire d'Application RMN, Faculté des Sciences Pharmaceutiques et Biologiques, Lille.
Cartigny
B
B
Imbenotte
M
M
Vallée
L
L
Vermeersch
G
G
Lhermitte
M
M
D000140:Acidosis, Lactic / Q000139:chemically induced; D000293:Adolescent; D000328:Adult; D000927:Anticonvulsants / Q000737:chemistry / Q000378:metabolism / Q000506:poisoning* / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005965:Glucuronates / Q000737:chemistry / Q000652:urine; D020723:Glucuronic Acid; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011041:Poisoning / Q000175:diagnosis; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*; D014635:Valproic Acid / Q000737:chemistry / Q000378:metabolism / Q000506:poisoning* / Q000652:urine*
D000927:Anticonvulsants; D005965:Glucuronates; D014635:Valproic Acid; D020723:Glucuronic Acid