A new insight into the Ag+ and Cu+ binding sites in the metallothionein beta domain.
The copper(I) and silver(I) binding properties of the beta fragment of recombinant mouse metallothionein I have been studied by electronic absorption and circular dichroism spectroscopy. When possible, the stoichiometry of the species formed was confirmed by electrospray mass spectrometry. The behaviour observed differs from that reported for the native protein. Titration of either Zn3-beta MT at pH 7 or apo-beta MT at pH 3 with Cu+ leads to the formation of species having the same stoichiometry and structure: Cu6-beta MT, Cu7-beta MT and Cu10-beta MT. In the first stage of the titration of Zn3-beta MT with Cu+ at pH 7 one additional species of formula Cu4Zn1-beta MT was detected. In contrast, the titration of Zn3-beta MT at pH 7.5 and of apo-beta MT at pH 2.5 with Ag+ proceeds through different reaction pathways, affording ZnxAg3-beta MT, Ag6-beta MT and Ag9-beta MT or Ag3-beta MT, Ag6-beta MT and Ag9-beta MT, respectively. The CD envelope corresponding to species with the same stoichiometric ratio, Ag6-beta MT and Ag9-beta MT, indicates that they have a different structure at each pH value. On the basis of the differences observed, the postulated similarity between copper and silver binding to metallothionein may be questioned.
Bofill
R
R
Departament de Química, Facultat de Ciencies, Universitat Autònoma de Barcelona, Spain.
Palacios
O
O
Capdevila
M
M
Cols
N
N
González-Duarte
R
R
Atrian
S
S
González-Duarte
P
P
D000818:Animals; D001665:Binding Sites; D002942:Circular Dichroism; D003300:Copper / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008668:Metallothionein / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012834:Silver / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D010446:Peptide Fragments; D011994:Recombinant Proteins; D012834:Silver; D003300:Copper; D008668:Metallothionein