Non-proteolytic activation of human prorenin by anti-prorenin prosegment (pf#1: 1P-15P) antiserum.
Recombinant human prorenin was activated by incubation with anti-prorenin prosegment (L1PPTDTTTFKRIFLKR15P) antiserum at 4 degrees C. This activation was dependent on the concentration of the antiserum and incubation time. After the activation no molecular weight alteration of prorenin was observed by immunoblotting analysis. A peptide of L1PPTDTTTF8P as well as L1PPTDTTTFKRIFLKR15P potently interfered with the activation. Most of the activated prorenin bound to Protein A Sepharose CL 4B. The Km and Vmax values of the activated prorenin were 0.2 microM and 23.7 micrograms Ang I/ml/h, respectively, which were similar in level to those of mature renin obtained by trypsinization.
Suzuki
F
F
Molecular Genetics Research Center, Gifu University, Japan. aob3073@cc.gifu-u.ac.jp
Hatano
Y
Y
Nakagawa
T
T
Terazawa
K
K
Gotoh
A
A
Nasir
U M
UM
Ishida
Y
Y
Nakamura
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D003080:Cold Temperature; D006224:Cricetinae; D004789:Enzyme Activation / Q000276:immunology; D004792:Enzyme Precursors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012083:Renin / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D004792:Enzyme Precursors; D007106:Immune Sera; D010446:Peptide Fragments; D012083:Renin