Regulation of steroidogenesis in jc-410, a stable cell line of porcine granulosa origin.
We investigated the regulation of steroidogenesis in a cell line of porcine granulosa origin, JC-410. Cells responded to the protein kinase-A activators, cholera toxin and forskolin, with increased accumulation of intracellular cAMP. Histochemically, cells were shown to contain 3beta-HSD, the enzyme which converts pregnenolone to progesterone. The JC-410 cells produced progesterone and responded to the protein kinase-A activators with an increase in progesterone synthesis. Progesterone levels were also increased by 25-hydroxycholesterol, pregnenolone, estradiol and androstenedione. Follicle-stimulating hormone and luteinizing hormone had no effect on cAMP or progesterone accumulation. Androstenedione was required for the synthesis of estradiol by JC-410 cells. Steady-state levels of mRNA for the steroidogenic enzymes 3beta-HSD and P450scc were increased by treatment with cholera toxin, whereas P450arom was not changed. These cells express the steroidogenic enzymes genes in a similar fashion to primary cultures of porcine granulosa cells. The JC-410 cells may represent a valuable model to study second messenger regulation and the molecular mechanisms involved in steroidogenesis in granulosa cells.
Rodway
M R
MR
Department of Obstetrics Gynecology and Reproductive Biology, Toxicology Centre, Saskatoon, SK, Canada.
Swan
C L
CL
Gillio-Meina
C
C
Crellin
N K
NK
Flood
P F
PF
Chedrese
P J
PJ
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006888:Hydroxycholesterols / Q000494:pharmacology; D007700:Kinetics; D011284:Pregnenolone / Q000378:metabolism / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects
D006888:Hydroxycholesterols; D005576:Colforsin; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D011284:Pregnenolone; C007997:25-hydroxycholesterol; D002772:Cholera Toxin; D000242:Cyclic AMP; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme; D017868:Cyclic AMP-Dependent Protein Kinases