An association between lipid peroxidation and alpha-naphthylisothiocyanate-induced liver injury in rats.
An association between lipid peroxidation and alpha-naphthylisothiocyanate (ANIT)-induced liver injury was examined in rats injected once with the toxicant (75 mg/kg body weight). The severity of liver injury was estimated 12, 24, 48, and 72 h after ANIT injection. Liver injury appeared 24 h after ANIT injection, progressed at 48 h, and recovered at 72 h, judging from the serum levels of marker enzymes and components. Serum lipid peroxide (LPO) concentration increased 24 h after ANIT injection and further increased at 48 h, but this increase was attenuated at 72 h. In contrast, liver LPO content increased 12 h after ANIT injection and further increased 24 and 48 h, but this increase was attenuated at 72 h. Similarly, myeloperoxidase (MPO) activity, an index of neutrophil infiltration, in the liver tissue increased 12 h after ANIT injection and further increased at 24 and 48 h, but this increase was attenuated at 72 h. Either serum LPO concentration or liver LPO content was significantly correlated with liver MPO activity (r = 0.661 for serum LPO concentration; r = 0.585 for liver LPO content). These results suggest that lipid peroxidation might be associated with ANIT-induced liver injury in rats and that this lipid peroxidation might occur via oxygen radicals derived from neutrophils infiltrated into the liver tissue of ANIT-intoxicated rats.
Kongo
M
M
Department of Biochemistry, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.
Ohta
Y
Y
Nishida
K
K
Sasaki
E
E
Harada
N
N
Ishiguro
I
I
D015058:1-Naphthylisothiocyanate / Q000008:administration & dosage / Q000009:adverse effects*; D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D002779:Cholestasis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013223:Statistics as Topic; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000187:drug effects
D001647:Bile Acids and Salts; D008054:Lipid Peroxides; D015058:1-Naphthylisothiocyanate; D009195:Peroxidase; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase; D001663:Bilirubin