title: Mast cells and gut nerve development: implications for Hirschsprung's disease and intestinal neuronal dysplasia.
abstract: OBJECTIVE
  In Hirschsprung's disease (HD), the aganglionic bowel is characterized by the presence of hypertrophic nerve trunks and increased numbers of adrenergic and cholinergic nerve fibers. Intestinal neuronal dysplasia (IND), if associated with HD, occurs proximal to the aganglionic segment in HD, and is characterized by dysplasia of parasympathetic nerves, hyperganglionosis, and giant ganglia. However, the cause of such abnormalities in HD and IND is unclear. Recent reports that mast cells (MC) have been observed in direct contact with nerve fibers generally, suggest that MC are essential for nerve growth and repair. MC synthesize, store, and release nerve growth factor (NGF). NGF supports the development and functional maintenance of sympathetic and cholinergic neurons. The aim of this study was to examine the colonic distribution of MC with respect to nerves in HD and HD associated with IND.
  
  
  METHODS
  MC and NGF were examined immunohistochemically in ganglionic, transitional, and aganglionic segments of colon from 20 patients with HD (five patients associated with IND) and 15 age-matched controls. MC were counted in each of five random fields using light microscopy (x100).
  
  
  RESULTS
  Interestingly, aganglionic and IND segments had large numbers of MC in all layers compared with ganglionic segments in HD patients and controls (P< .0001). The number of MC in transitional segments was significantly less compared with ganglionic segments in HD patients and controls (P< .01). MC stained positively for NGF, and some were found in contact with abnormal hypertrophic nerve trunks in HD and giant ganglia in IND.
  
  
  CONCLUSIONS
  MC may cause hypertrophic nerve trunks and giant ganglia by releasing NGF and also may be an important factor in the excessive development of cholinergic and adrenergic nerve fibers in HD and IND.
authors.affiliation: Department of Pediatric Surgery, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: A
authors.initials: A
authors.lastname: Yamataka
authors.forename: T
authors.initials: T
authors.lastname: Fujimoto
authors.forename: G J
authors.initials: GJ
authors.lastname: Lane
authors.forename: T
authors.initials: T
authors.lastname: Miyano
mesh_terms: D003106:Colon / Q000294:innervation*; D017615:Enteric Nervous System / Q000254:growth & development* / Q000473:pathology; D006627:Hirschsprung Disease / Q000209:etiology* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008407:Mast Cells / Q000502:physiology*; D009412:Nerve Fibers / Q000737:chemistry / Q000473:pathology; D009414:Nerve Growth Factors / Q000502:physiology
chemical_list: D009414:Nerve Growth Factors