Specific numerical chromosomal aberrations induced by adriamycin.
Fluorescence in situ hybridization with 7, 17, X, and Y chromosome-specific DNA probe was used to investigate the ability of Adriamycin (AM) to induce aneuploidy in interphase human lymphocytes. The reliability of the probes was tested by hybridization to metaphases and interphase nuclei of untreated normal lymphocytes. Two signals were scored in over 87% of the analyzed nuclei with chromosome 7 and 17 probes, whereas one signal was recorded in over 86% of the nuclei with chromosomes X and Y. The same conditions and probe concentrations were used for hybridizing the four probes to interphase nuclei of AM-treated and untreated lymphocytes, cultured from healthy individuals and cancer patients. AM was found to induce significant increases of trisomy 7 and 17 in lymphocytes cultured from healthy individuals and cancer patients, where the interphase nuclei showed three spots in over 70% and 72% of the cells, respectively. Only 6% of interphase nuclei of untreated cells cultured from healthy individuals and cancer patients showed three spots. No significant increase in X or Y aneuploidy was induced by exposure to AM.
Aly
M S
MS
Zoology Department, Faculty of Science, Cairo University, Egypt.
Othman
O E
OE
El Nahas
S M
SM
D000328:Adult; D000782:Aneuploidy; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002478:Cells, Cultured; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D015342:DNA Probes; D004317:Doxorubicin / Q000633:toxicity*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D014960:X Chromosome; D014998:Y Chromosome
D000900:Anti-Bacterial Agents; D015342:DNA Probes; D004317:Doxorubicin