Three-dimensional cultures of prostatic cells: tissue models for the development of novel anti-cancer therapies.
This review addresses the application of three-dimensional cultures of prostatic cells to the development of novel anti-cancer therapies. A variety of therapeutic agents to combat prostate cancer are currently under development. These include cytotoxins, differentiation agents and, more recently, genetically modified tumor vaccines. Three-dimensional cultures of prostatic cells are increasingly used in preclinical research in the design of new therapies and in the development of delivery strategies for these treatments. These tissue-like structures more realistically model the structural architecture and differentiated function of the human prostate than a cellular monolayer. In doing so, three-dimensional cultures produce an in vivo-like response to therapeutic agents. Advances in tissue engineering have improved the variety, fidelity and quantity of these prostate models. To date, they have been applied to estimate the dose of new drug therapies, evaluate drug penetration into solid tumors, assess the effectiveness of drug combinations, and develop tumor vaccines.
O'Connor
K C
KC
Department of Chemical Engineering, Tulane University, New Orleans, Louisiana 70118, USA. koc@mailhost.tcs.tulane.edu
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000628:therapy*; D018874:Spheroids, Cellular