title: Plasma levels of selectins and interleukins in cardiovascular surgery using cardiopulmonary bypass.
abstract: BACKGROUND
  Cardiovascular surgery with cardiopulmonary bypass (CPB) leads to activation of a variety of inflammatory pathways, including the release of cytokines and selectins.
  
  
  METHODS
  In 20 patients undergoing elective coronary artery bypass grafting, plasma levels of interleukins IL-2, -6, -8, -10, -12 and of P-, E-, and L-selectins were measured at eight time points before, during, and after CPB using a standardized ELISA technique.
  
  
  RESULTS
  IL-2 plasma levels decreased significantly after the start of CPB and remained low until the second postoperative day. IL-6 and IL-8 levels increased significantly after weaning off CPB, with mean peak values six hours postoperatively. Very low IL-10 plasma levels were detectable preoperatively. They remained low during CPB and peaked significantly after weaning off CPB until skin closure. The IL-12 levels decreased after weaning off CPB (p < 0.05) until 6 hours postoperatively. The plasma levels of P-selectin showed no alterations, but concentrations of E- and L-selectin decreased after the start of CPB (p < 0.05). There were no adverse postoperative events.
  
  
  CONCLUSIONS
  The results of our study demonstrate a dysregulation of cytokine and selectin production during and up to 48 h after CPB, which may be a "normal" stress reaction to CPB.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig University, Giessen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sablotzki
authors.forename: M G
authors.initials: MG
authors.lastname: Dehne
authors.forename: V
authors.initials: V
authors.lastname: Mann
authors.forename: G
authors.initials: G
authors.lastname: Görlach
authors.forename: J
authors.initials: J
authors.lastname: Mühling
authors.forename: B
authors.initials: B
authors.lastname: Zickmann
authors.forename: G
authors.initials: G
authors.lastname: Hempelmann
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass / Q000379:methods*; D003327:Coronary Disease / Q000097:blood* / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology; D007378:Interleukins / Q000097:blood*; D007432:Intraoperative Period; D011184:Postoperative Period; D011379:Prognosis; D019087:Selectins / Q000097:blood*
chemical_list: D015415:Biomarkers; D007378:Interleukins; D019087:Selectins