title: Strain dependency of the immunopotentiating activity of Lactobacillus delbrueckii subsp. bulgaricus.
abstract: To obtain strains of Lactobacillus delbrueckii subsp. bulgaricus with high immunopotentiating activity, we screened 90 strains of this bacterial species for the proliferative response of murine spleen and beta-lactoglobulin-primed lymph node cells. In this screening, certain strains showed strong immunopotentiating activity. Among them, strain 1023 had the strongest mitogenic activity for murine Peyer's patch (PP) cells. Furthermore, strain 1023 induced IgA antibody production by PP cells as strongly as Bifidobacterium longum 6001, which had adjuvant activity when orally administered. Also in the assays using immune cells from human-flora-associated mice a few strains including 1023 showed strong immunopotentiating activity comparable to B. longum 6001. These results suggest that L. delbrueckii subsp. bulgaricus strains such as 1023 may be useful for the production of fermented milk with a more beneficial effect on the systemic and mucosal immune system.
authors.affiliation: Nutrition Science Institute, Meiji Milk Products Co., Ltd., Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nagafuchi
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Yajima
authors.forename: T
authors.initials: T
authors.lastname: Kuwata
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
authors.forename: K
authors.initials: K
authors.lastname: Itoh
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D004731:Endotoxins / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D066298:In Vitro Techniques; D007778:Lactobacillus / Q000737:chemistry / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D004731:Endotoxins; D007070:Immunoglobulin A; D007782:Lactoglobulins; D008070:Lipopolysaccharides; D003208:Concanavalin A; C044101:endotoxin, Escherichia coli