Chemokine Up-regulation and activated T cell attraction by maturing dendritic cells.
Langerhans' cells migrating from contact-sensitized skin were found to up-regulate expression of macrophage-derived chemokine (MDC) during maturation into lymph node dendritic cells (DCs). Naïve T cells did not migrate toward MDC, but antigen-specific T cells rapidly acquired MDC responsiveness in vivo after a subcutaneous injection of antigen. In chemotaxis assays, maturing DCs attracted activated T cells more strongly than naïve T cells. These studies identified chemokine up-regulation as part of the Langerhans' cell maturation program to immunogenic T cell-zone DC. Preferential recruitment of activated T cells may be a mechanism used by maturing DCs to promote encounters with antigen-specific T cells.
Tang
H L
HL
Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA.
Cyster
J G
JG
D000818:Animals; D002478:Cells, Cultured; D054415:Chemokine CCL19; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000096:biosynthesis* / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D015854:Up-Regulation
C515461:Ccl19 protein, mouse; C515483:Ccl22 protein, mouse; D054415:Chemokine CCL19; D054422:Chemokine CCL22; D019742:Chemokines, CC; D016650:Fluorescein-5-isothiocyanate