title: Characterization of a haemolytic factor from Candida albicans.
abstract: The culture supernatant of Candida albicans promoted the disruption of human red blood cells (RBCs). The haemolytic activity was detected in a sugar-rich fraction (about 200 kDa) from Sephacryl S-100 chromatography. As the haemolytic activity was adsorbed by concanavalin A-Sepharose, the haemolytic factor may be a mannoprotein. The activity was inactivated by periodate oxidation, indicating that the sugar moiety of the mannoprotein played an important role in the haemolysis. The structure of the sugar moiety of the mannoprotein was identified as a cell-wall mannan by 1H-NMR analysis, and purified C. albicans mannan promoted the disruption of RBCs. The binding of mannan to RBCs was demonstrated by flow cytometric analysis and was inhibited by the addition of band 3 protein inhibitor, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. These results indicated that a mannoprotein released from C. albicans bound to the band 3 protein on RBCs, thereby promoting their disruption.
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Takano
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Murakami
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsuhisa
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakao
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mikami
authors.affiliation: Sendai Research Institute for Mycology, 14-34 Toshogu 1 Chome, Aobaku, Sendai 981-0908, Japan.
authors.forename: Masuko
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000838:Anions / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002176:Candida albicans / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000302:isolation & purification* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D013388:Succinimides / Q000494:pharmacology
chemical_list: D000838:Anions; D008351:Mannans; D008562:Membrane Glycoproteins; D013388:Succinimides; C025363:mannoproteins; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D006859:Hydrogen; C035760:bis(sulfosuccinimidyl)suberate; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid