title: The C-terminal sequence of the lambda holin constitutes a cytoplasmic regulatory domain.
abstract: The C-terminal domains of holins are highly hydrophilic and contain clusters of consecutive basic and acidic residues, with the overall net charge predicted to be positive. The C-terminal domain of lambda S was found to be cytoplasmic, as defined by protease accessibility in spheroplasts and inverted membrane vesicles. C-terminal nonsense mutations were constructed in S and found to be lysis proficient, as long as at least one basic residue is retained at the C terminus. In general, the normal intrinsic scheduling of S function is deranged, resulting in early lysis. However, the capacity of each truncated lytic allele for inhibition by the S107 inhibitor product of S is retained. The K97am allele, when incorporated into the phage context, confers a plaque-forming defect because its early lysis significantly reduces the burst size. Finally, a C-terminal frameshift mutation was isolated as a suppressor of the even more severe early lysis defect of the mutant SA52G, which causes lysis at or before the time when the first phage particle is assembled in the cell. This mutation scrambles the C-terminal sequence of S, resulting in a predicted net charge increase of +4, and retards lysis by about 30 min, thus permitting a viable quantity of progeny to accumulate. Thus, the C-terminal domain is not involved in the formation of the lethal membrane lesion nor in the "dual-start" regulation conserved in lambdoid holins. Instead, the C-terminal sequence defines a cytoplasmic regulatory domain which affects the timing of lysis. Comparison of the C-terminal sequences of within holin families suggests that these domains have little or no structure but act as reservoirs of charged residues that interact with the membrane to effect proper lysis timing.
authors.affiliation: Institute of Microbiology and Genetics, Vienna Biocenter, 1030 Vienna, Austria.
authors.forename: U
authors.initials: U
authors.lastname: Bläsi
authors.forename: P
authors.initials: P
authors.lastname: Fraisl
authors.forename: C Y
authors.initials: CY
authors.lastname: Chang
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Young
mesh_terms: D000595:Amino Acid Sequence; D001433:Bacteriolysis / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000821:virology*; D008565:Membrane Proteins / Q000235:genetics*; D008961:Models, Structural; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D013997:Time Factors; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D008565:Membrane Proteins; C108624:S holin, bacteriophage lambda; D014764:Viral Proteins; C082002:bacteriophage lambda lysis inhibitor protein S107