title: The hatching gland cells of trout embryos: characterisation of N- and O-linked oligosaccharides.
abstract: A histochemical, light and electron microscopy study of the hatching gland cells (HGCs) in incubated 50-d-old trout embryos is reported. The distribution of carbohydrate residues in the glycoconjugates of these cells was studied by means of a battery of 13 different lectins conjugated with horseradish peroxidase (PNA, ConA, LCA, WGA, SBA, UEA-I, HPA, DBA) or digoxigenin (DSA, MAA, AAA, SNA, GNA). Identification of N- and O-linked oligosaccharides in HGCs was performed by application of both chemical and enzymatic treatments. Present results suggest that HGCs are seromucous cells which store both high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE), and that their cytoplasmic granules, endoplasmic reticulum and Golgi complex contain additional sialic acid-rich glycoproteins. The negative charge of these glycoproteins might be responsible for the rapid expansion of mucin to form a highly hydrated gel, which would facilite the action of these enzymes in programmed cell death and might play a major role during the morphogenic events.
authors.affiliation: Department of Anatomy and Embryology, Faculty of Veterinary Medicine, UCM, University of Alcala, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: De Gaspar
authors.forename: M J
authors.initials: MJ
authors.lastname: Blanquez
authors.forename: B
authors.initials: B
authors.lastname: Fraile
authors.forename: R
authors.initials: R
authors.lastname: Paniagua
authors.forename: M I
authors.initials: MI
authors.lastname: Arenas
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000737:chemistry; D018870:Endoplasmic Reticulum, Rough / Q000737:chemistry; D004792:Enzyme Precursors / Q000032:analysis; D006056:Golgi Apparatus / Q000737:chemistry; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins / Q000378:metabolism; D008854:Microscopy, Electron; D009844:Oligosaccharides / Q000032:analysis*; D014337:Trout / Q000196:embryology*
chemical_list: D004792:Enzyme Precursors; D037102:Lectins; D009844:Oligosaccharides