title: Genetic variation in Beauveria bassiana populations associated with the darkling beetle, Alphitobius diaperinus.
abstract: A study was conducted to assess genetic variation within and among populations of Beauveria bassiana (Deuteromycotina: Hyphomycetes) associated with the darkling beetle, Alphitobius diaperinus (Coleoptera: Tenebrionidae), using RAPD markers. A hierarchical collection of samples (strains from the same insect specimen, from insects from the same location, and from insects from different locations) was obtained from infected beetles from North Carolina (NC) and West Virginia (WV), USA. Ten primers resolved 81 strains into 80 distinct multiband phenotypes reflecting the substantial amount of variation that was present. Variation present within populations was evident not only in the separation of each strain as a distinct multiband phenotype but also in the separation of strains within a population into separate clusters. Among populations, a group sharing more than 89% similarity was observed among all the strains from Martin Co. and Greene Co., NC and 61% of the strains collected from WV. Some genetic differentiation was present among the other populations but the separation was not distinct with a few strains from some populations showing greater affinity to strains from other collection sites.
authors.affiliation: Department of Entomology, North Carolina State University, Raleigh, North Carolina 27695, USA. castrila@muohio.edu
authors.forename: L A
authors.initials: LA
authors.lastname: Castrillo
authors.forename: B M
authors.initials: BM
authors.lastname: Wiegmann
authors.forename: W M
authors.initials: WM
authors.lastname: Brooks
mesh_terms: D000818:Animals; D020171:Botrytis / Q000145:classification / Q000235:genetics*; D001517:Coleoptera / Q000382:microbiology*; D014644:Genetic Variation