title: Genetic variation in the VP4 and NSP4 genes of human rotavirus serotype 3 (G3 type) isolated in China and Japan.
abstract: Sequence analyses of the VP4 and NSP4 genes were performed on twenty human isolates of serotype G3 rotavirus obtained from China and Japan. One isolate from China, CHW17, possessed P[4] genotype VP4 and KUN group NSP4 genes which are associated with G2. One isolate (02/92) from Japan, which was shown to have a wider spacing between RNA segments 10 and 11 by RNA polyacrylamide gel electrophoretic analysis like AU-1, possessed P[9] genotype VP4 and AU-1 group NSP4 genes. The other isolates had P[8] genotype VP4 and Wa group NSP4 genes. While the nucleotide sequence conservation among the G3 VP7 genes was more than 79% (Wen et al, Arch. Virol., 1997, 142: 1481-1489), the conservation of VP4 and NSP4 genes in the same genotypes or groups was more than 85%.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Japan.
authors.forename: X R
authors.initials: XR
authors.lastname: Cao
authors.forename: S
authors.initials: S
authors.lastname: Akihara
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Fang
authors.forename: O
authors.initials: O
authors.lastname: Nakagomi
authors.forename: H
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002681:China; D004279:DNA, Viral; D005814:Genes, Viral; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology; D012703:Serotyping; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; D017361:Viral Nonstructural Proteins