title: Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers.
abstract: Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co-glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.
authors.affiliation: MRC Clinical Sciences Centre, Tuberculosis & Related Infections Unit, Hammersmith Hospital, London, UK.
authors.forename: N
authors.initials: N
authors.lastname: Venkataprasad
authors.forename: A G
authors.initials: AG
authors.lastname: Coombes
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: M
authors.initials: M
authors.lastname: Rohde
authors.forename: K
authors.initials: K
authors.lastname: Wilkinson
authors.forename: F
authors.initials: F
authors.lastname: Hudecz
authors.forename: S S
authors.initials: SS
authors.lastname: Davis
authors.forename: H M
authors.initials: HM
authors.lastname: Vordermeier
mesh_terms: D000327:Adsorption; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D003460:Crystallization; D004247:DNA / Q000096:biosynthesis; D004337:Drug Carriers; D005260:Female; D007114:Immunization; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011091:Polyesters / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry; D018417:Th1 Cells / Q000276:immunology*
chemical_list: C075966:Ag38 antigen, Mycobacterium tuberculosis; D000942:Antigens, Bacterial; D004337:Drug Carriers; D008074:Lipoproteins; D010446:Peptide Fragments; D011091:Polyesters; D015847:Interleukin-4; D011098:Polyglactin 910; C033616:poly(lactide); D007371:Interferon-gamma; D004247:DNA