title: V3 serotyping of HIV-1 infection: correlation with genotyping and limitations.
abstract: HIV-1 V3 serotyping is a classification of immunodeficiency viruses based on antibody binding to V3 peptides that allows obtaining information on circulating subtypes that could be important for population-based epidemiologic studies. Recently, several laboratories have developed V3 enzyme-immunoassays (EIAs) using V3 peptides of subtypes A to E. In the present study, the utility of including additional peptides of subtypes F to H to the EIA was evaluated on a panel of 203 well-characterized serum samples from patients with diverse geographic origins (22 countries) and known HIV-1 genotype (79 A, 61 B, 21 C, 7 D, 7 E, 21 F, 6 G, 1 H). The results indicate a high predictive value (ppv) for serotypes B (> or =0.86), D (1) and E (0.88), and confirm the difficulty of predicting genotype A or C based on serotype A or C. Results also indicate that inclusion of the F peptide in the V3 EIAs may be useful (ppv = 0.61), but introduction of peptides G and H failed to demonstrate significant sensitivity or specificity for these subtypes. Correlation between serotyping and amino-acid sequences of the V3 region from 103 samples allowed the identification of key amino-acids that appear essential for subtype-specific seroreactivity.
authors.affiliation: Laboratoire de Virologie, Université François Rabelais, Tours, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Plantier
authors.forename: F
authors.initials: F
authors.lastname: Damond
authors.forename: M
authors.initials: M
authors.lastname: Lasky
authors.forename: J L
authors.initials: JL
authors.lastname: Sankalé
authors.forename: C
authors.initials: C
authors.lastname: Apetrei
authors.forename: M
authors.initials: M
authors.lastname: Peeters
authors.forename: L
authors.initials: L
authors.lastname: Buzelay
authors.forename: S
authors.initials: S
authors.lastname: M'Boup
authors.forename: P
authors.initials: P
authors.lastname: Kanki
authors.forename: E
authors.initials: E
authors.lastname: Delaporte
authors.forename: F
authors.initials: F
authors.lastname: Simon
authors.forename: F
authors.initials: F
authors.lastname: Barin
mesh_terms: D000595:Amino Acid Sequence; D005260:Female; D005838:Genotype; D015699:HIV Envelope Protein gp120 / Q000145:classification* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000145:classification* / Q000276:immunology; D012703:Serotyping
chemical_list: D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments