title: The gene encoding a periplasmic deoxyribonuclease from Aeromonas hydrophila.
abstract: A gene encoding a deoxyribonuclease, dnsH, was cloned from Aeromonas hydrophila JMP636. The predicted mature protein was very similar to the previously described extracellular Dns from this organism and an N-terminal region corresponding to a large putative signal sequence was predicted for the JMP636 protein. Inactivation of dnsII demonstrated that the DnsH protein was not present extracellularly in this strain. As DnsH degraded plasmid DNA and was believed to have a periplasmic location, a dnsH mutant was constructed to determine whether electroporation of A. hydrophila with plasmid DNA could be achieved. No transformants were detected. From SDS-PAGE studies, at least two additional DNases remain to be characterised from A. hydrophila JMP636.
authors.affiliation: Department of Microbiology, University of Queensland, Australia.
authors.forename: H N
authors.initials: HN
authors.lastname: Dodd
authors.forename: J M
authors.initials: JM
authors.lastname: Pemberton
mesh_terms: D016980:Aeromonas hydrophila / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D018274:Electroporation; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D019897:Periplasm / Q000201:enzymology*; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004706:Endodeoxyribonucleases; C119833:DnsH protein, Aeromonas hydrophila