[Immunohistochemical characterisation of surgically excised choroidal neovascularisation in age-related macular degeneration].
OBJECTIVE
The objective of the study was to gather further information about the pathogenesis of choroidal neovascularisations (CNV), which is still not clearly understood, and to establish criteria for making decisions on a appropriate therapy. Immunohistochemical characteristation should allow a more comprehensive evaluation of cellular components of the membranes and their functional role.


METHODS
In 29 patients (16 women, 13 men) with age-related macular degeneration ranging in age from 46 to 91 years (mean age, 76.4 years), CNV were excised by pars-plana vitrectomy. Sections were stained with hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) and examined by light microscopy. For the immunohistochemical characterisation of the surgical specimens the following anti-genetic determinants were used: glial fibrillary acid protein (GFAP) for glial cells, synaptophysin for neuronal cells, neuron-specific enolase (NSE) for neuronal and neuroectodermal cells, CD 31 for endothelial cells and pancytokeratin (KL1) for cells of the retinal pigment epithelium (RPE). Cells undergoing apoptosis were labeled with the TUNEL technique.


RESULTS
22 (76%) surgical specimens showed TUNEL positive cells in the connective tissue, vascular endothelium and retinal pigment epithelium. Positive immunostaining of neuronal antigenetic determinants was found for glial fibrillary acid protein in 22 patients (76%), for synaptophysin in 28 patients (97%) and for neuron-specific enolase in 21 patients (72%) CNV. The epithelial marker KL1 was positive in 28 patients (97%) and the endothelial marker CD 31 in 20 patients (69%).


CONCLUSIONS
The immunohistochemical analyses of CNV showed that in the majority of cases during the excision of choroidal neovascularizations in addition to scar tissue and connective tissue also parts of the native retinal pigment epithelium and of the neurosensory retina are removed which is only partly visible with standard staining techniques. These findings suggest that the mostly not satisfying postoperative results are partly due to the damage of neuronal cells and a partial loss of the retinal pigment epithelium. Apoptosis as a regulating mechanism in choroidal neovascularization. The variable appearence of apoptosis suggests that it is possibly related to the degree of activity of CNV.
Wiezorrek
R
R
Universitäts-Krankenhaus Eppendorf, Augenklinik, Hamburg.
Bialasiewicz
A A
AA
Schäfer
H
H
Richard
G
G
D000368:Aged; D000369:Aged, 80 and over; D020256:Choroidal Neovascularization / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D053938:DNA Fragmentation; D004475:Ectoderm / Q000737:chemistry / Q000201:enzymology; D004730:Endothelium, Vascular / Q000737:chemistry; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling / Q000379:methods; D007633:Keratins / Q000032:analysis; D008268:Macular Degeneration / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000737:chemistry; D009474:Neurons / Q000737:chemistry / Q000201:enzymology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000737:chemistry; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D016708:Synaptophysin / Q000032:analysis; D014821:Vitrectomy
D005904:Glial Fibrillary Acidic Protein; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D016708:Synaptophysin; D007633:Keratins; D010751:Phosphopyruvate Hydratase