Isolation and characterization of di- and tri-mannosyl-cyclomaltoheptaoses (beta-cyclodextrins) produced by reverse action of alpha-mannosidase from jack bean.
Di- and tri-mannosyl-cyclomaltoheptaoses (beta-cyclodextrins, beta CDs), which were synthesized together with monomannosyl-beta CD in a large-scale production by reverse action of alpha-mannosidase from jack bean, were isolated and purified by HPLC. The structures of seven isomers of di-mannosyl-beta CD and six isomers of tri-mannosyl-beta CD were elucidated by FABMS and NMR spectroscopy, and by enzymatic methods.
Koizumi
K
K
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Tanimoto
T
T
Okada
Y
Y
Takeyama
S
S
Hamayasu
K
K
Hashimoto
H
H
Kitahata
S
S
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000138:chemical synthesis / Q000302:isolation & purification*; D007887:Fabaceae / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008361:Mannosidases / Q000737:chemistry*; D010946:Plants, Medicinal; D016339:Spectrometry, Mass, Fast Atom Bombardment; D043323:alpha-Mannosidase; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D008361:Mannosidases; D043323:alpha-Mannosidase; C031215:betadex