Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae.
Pyruvate-decarboxylase (Pdc)-negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose-containing complex media, but not on defined glucose-containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cerevisiae does not exhibit prolonged growth on glucose. Pdc- strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA.
Flikweert
M T
MT
Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, The Netherlands.
de Swaaf
M
M
van Dijken
J P
JP
Pronk
J T
JT
D000085:Acetates / Q000378:metabolism; D000105:Acetyl Coenzyme A / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D008962:Models, Theoretical; D011767:Pyruvate Decarboxylase / Q000172:deficiency*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development*
D000085:Acetates; D003470:Culture Media; D000105:Acetyl Coenzyme A; D011767:Pyruvate Decarboxylase; D005947:Glucose