title: Functional roles of aromatic residues in the ligand-binding domain of cyclic nucleotide-gated channels.
abstract: The ligand-binding domains of cyclic nucleotide-gated (CNG) channels show sequence homology to corresponding region(s) of the Escherichia coli catabolite gene-activator protein (CAP) and to the regulatory subunit of cAMP-dependent or cGMP-dependent protein kinases. The structure of CAP and that of a cAMP-dependent protein kinases regulatory subunit have been solved, prompting efforts to generate structural models for the binding domains in CNG channel. These models explicitly predicted that an aromatic residue in the CNG channel aligning with leucine 61 of CAP forms an interaction with the bound cyclic nucleotide. We tested this hypothesis by site-directed mutagenesis in a rat olfactory channel (rOCNC1) and a bovine rod photoreceptor channel (Brcng). We found that mutations at this site had only weak effects that were not specific to the aromatic or the hydrophobic nature of the substituted residue. This result weakens the hypothesis of a strong or specific interaction at this site. We also separately mutated most of the other aromatic residues in the binding domain to alanine; most of these mutations resulted in channels that either did not function or had only minor changes in sensitivity. However, replacing tyrosine 565 with alanine (Y565A) in rOCNC1 increased agonist sensitivity by approximately 10-fold and resulted in prominent spontaneous activities. Y565 presumably lies between two alpha helices in the binding domain; one of these, the C helix, probably rotates during channel activation. The position of Y565 at the "hinge" between the C helix and another portion of the binding domain, and the consequences of Y565 mutations, strongly suggest that this portion of the binding domain is involved in channel gating processes.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: H A
authors.initials: HA
authors.lastname: Lester
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D054815:Cyclic Nucleotide-Gated Cation Channels; D004594:Electrophysiology; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014443:Tyrosine / Q000235:genetics* / Q000502:physiology
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; D007473:Ion Channels; D014443:Tyrosine