Loss of 3-nitrotyrosine on exposure to hypochlorous acid: implications for the use of 3-nitrotyrosine as a bio-marker in vivo.
Peroxynitrite (ONOO-) is a reactive nitrogen species which in vivo is often assessed by the measurement of free or protein bound 3-nitrotyrosine. Indeed, 3-nitrotyrosine has been detected in many human diseases. However, at sites of inflammation there is also production of the powerful oxidant hypochlorous acid (HOCl) formed by the enzyme myeloperoxidase. Low concentrations of HOCl (<30 microM) caused significant and rapid loss (<10 minutes) of free and protein bound 3-nitrotyrosine. In contrast, no loss of 3-nitrotyrosine was observed with hydrogen peroxide, hydroxyl radical, or superoxide generating systems. Therefore, under conditions where there is concomitant peroxynitrite and hypochlorous acid formation, such as at sites of chronic inflammation, it is possible that HOCl removes 3-nitrotyrosine. This may have implications when assessing the role of reactive nitrogen species in disease conditions and could account for some of the discrepancies reported between 3-nitrotyrosine levels in tissues.
Whiteman
M
M
Pharmacology Group, University of London, Kings College, Manresa Road, London, SW3 6LX, England.
Halliwell
B
B
D015415:Biomarkers; D006997:Hypochlorous Acid / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D015415:Biomarkers; D017382:Reactive Oxygen Species; C002744:3-nitrotyrosine; D014443:Tyrosine; D006997:Hypochlorous Acid