title: No-touch isolation technique reduces intraoperative shedding of tumor cells into the portal vein during resection of colorectal cancer.
abstract: BACKGROUND
  The mutant-allele-specific amplification (MASA) method is capable of detecting 1 genetically altered tumor cell among thousands of normal cells. The MASA enabled us to detect occult tumor cells undetectable by histopathologic examination of lymph nodes and blood samples.
  
  
  METHODS
  To investigate whether tumor manipulation during operation enhances cancer cell dissemination into the portal vein with use of MASA and to assess the effect of the no-touch isolation technique in the treatment of colorectal cancers, 27 colorectal cancers (17 were operated on conventionally and 10 were operated on according to the no-touch isolation technique) were screened for mutations in K-ras or p53. We next examined blood samples of the portal vein collected before, during, and after manipulation of tumors, using MASA to look for the specific mutation found in the primary tumors.
  
  
  RESULTS
  Somatic mutations were identified in 18 of these primary tumors (11 were in the conventional resection technique group and 7 were in the no-touch isolation technique group). In 8 of 11 (73%) conventional resection technique cases, we identified the same genetic alteration of the primary tumor in the portal blood during operation, whereas only 1 patient (14%) in the no-touch isolation technique group had a positive result.
  
  
  CONCLUSIONS
  The no-touch isolation technique may be useful to prevent cancer cells from being shed into the portal vein during surgical manipulation.
authors.affiliation: Department of Surgery II, Kumamoto University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Egami
authors.forename: M
authors.initials: M
authors.lastname: Kai
authors.forename: Y
authors.initials: Y
authors.lastname: Kurusu
authors.forename: S
authors.initials: S
authors.lastname: Takano
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D000483:Alleles; D014408:Biomarkers, Tumor / Q000097:blood; D003082:Colectomy / Q000379:methods*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000556:secondary* / Q000601:surgery*; D004273:DNA, Neoplasm / Q000032:analysis / Q000097:blood; D006801:Humans; D007431:Intraoperative Complications / Q000517:prevention & control; D009360:Neoplastic Cells, Circulating; D015345:Oligonucleotide Probes; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011169:Portal Vein
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes