Binding of the Mn(III) complex of meso-tetrakis (4-N-methyl-pyridiniumyl) porphyrin to DNA. Effect of ionic strength.
Interactions of the water-soluble Mn(III) complex of meso-tetrakis (4-N-methyl-pyridiniumyl) porphyrin (Mn(III)TMPyP) with DNA in aqueous solutions at low (0.01 M) and high (0.2 M) ionic strengths have been studied by optical absorption, resonance light scattering (RLS) and 1H NMR spectroscopies. Optical absorption and RLS measurements have demonstrated that in DNA solutions at low ionic strength the Mn(III)TMPyP form aggregates, which are decomposed at DNA excess. At high ionic strength the aggregation was not observed. We explain this effect by assuming that upon increase in ionic strength, Mn(III) TMPyP dislocates from the DNA sites, which produces better conditions for the porphyrin aggregation, to sites where the aggregation is hindered. The 1H NMR data demonstrated that the aggregation observed at low ionic strength reduces the paramagnetism of Mn(III)TMPyP. This phenomenon was not observed at the high ionic strength in the absence of aggregation.
Gandini
S C
SC
Instituto de Química de São Carlos, Universidade de São Paulo, Brazil.
Yushmanov
V E
VE
Perussi
J R
JR
Tabak
M
M
Borissevitch
I E
IE
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000737:chemistry*; D009994:Osmolar Concentration; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D011522:Protons; D012542:Scattering, Radiation
D011166:Porphyrins; D011522:Protons; C092399:meso-tetrakis(1-methyl-4-pyridiniumyl)porphyrin; D008345:Manganese; D004247:DNA