title: An SHV-derived extended-spectrum beta-lactamase in Pseudomonas aeruginosa.
abstract: A clinical isolate of Pseudomonas aeruginosa RP-1 produced the extended-spectrum beta-lactamase (ESBL) SHV-2a. Its gene was expressed from a composite promoter made of the -35 region derived from the left inverted repeat of IS26 and the -10 region from the blaSHV-2a promoter itself. The DNA sequences immediately surrounding blaSHV-2a were homologous to plasmid pMPA2a from Klebsiella pneumoniae KpZU-3, while further away and 3' to the blaSHV-2a gene, a sequence corresponding to the left end of Tn1721 was detected, thus indicating a likely enterobacterial origin of this ESBL gene.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France.
authors.forename: T
authors.initials: T
authors.lastname: Naas
authors.forename: L
authors.initials: L
authors.lastname: Philippon
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: E
authors.initials: E
authors.lastname: Ronco
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D001483:Base Sequence; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases