[Use of protein-polysaccharide Brucella antigen labeled with colloidal gold for detection of specific antibodies by the dot-immunoassay method].
Antibrucella antibodies were detected by dot-immunoassay with colloid gold label of antigen. The specific antigen was protein-polysaccharide complex (PPC) isolated from vaccine strain Brucella abortus 19 BA by acetic acid hydrolysis of bacterial cells. Dot immunoassay with PPC labeled with colloid gold was effective in testing cattle, rabbit, and human sera for antibrucella antibodies. The proposed test system is simple, economic, highly sensitive and specific, and requires no expensive equipment and reagents, which are its advantages over routine serological tests.
Zagoskina
T Iu
TIu
Kalinovskiĭ
A I
AI
Markov
E Iu
EIu
Esina
N L
NL
Golubinskiĭ
E P
EP
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D017979:Gold Colloid / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017979:Gold Colloid; D011135:Polysaccharides, Bacterial