Development of neutralising human recombinant antibodies to pertussis toxin.
A phage antibody display library of single chain Fv (scFv) was derived from the peripheral blood of two patients recently recovered from pertussis infection. Ten scFv, differentiated by DNA fingerprinting, were isolated by panning the library against pertussis toxin. One scFv (type 1) accounted for 33% of clones after panning. Six of the panned scFv bound to pertussis toxin. The ability of the scFv to neutralise pertussis toxin was assessed using the Chinese hamster ovary cell assay. The predominant scFv (type 1) and two others (types IV and VIII) were able to neutralise the pertussis toxin.
Williamson
P
P
University Department of Medical Microbiology, Manchester Royal Infirmary, UK.
Matthews
R
R
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D009500:Neutralization Tests; D037342:Pertussis Toxin; D011993:Recombinant Fusion Proteins / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology*; D014917:Whooping Cough / Q000276:immunology
D000907:Antibodies, Bacterial; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; C055966:immunoglobulin Fv; D037342:Pertussis Toxin