Effect of alpha 1-acidic glycoprotein in the ascitic fluid of cancer patients on human NK cells: selective suppression of interferon-induced NK activation.
The in vitro effect of C-AGP (pure alpha1-acid glycoprotein from the ascitic fluid of cancer patients) on NK cell cytotoxicity was tested using normal healthy human PBMC. C-AGP had no inhibitory effect on basal NK cell activity. C-AGP selectively suppressed the augmentation of NK cell activity by rIFNalphaA and rIFNgamma, but C-AGP did not prevent the NK activation by rIL-2. NK cells in PBMC treated with C-AGP for 12 h and then washed just once, to remove the C-AGP, fully recovered the ability to respond to rIFNalphaA. However, after the treatment of PBMC with C-AGP for 5 or 6 days, NK cells failed to respond to rIFNalphaA, in spite of washing to remove C-AGP from the cultures. Monocytes were necessary for the suppressive effect of C-AGP on rIFNalphaA activation of NK cells. Indomethacin restored the ability of NK cells to respond to rIFNalphaA in C-AGP-treated PBMC. These results suggest that monocytes are able to selectively suppress the response of NK cells to IFNs in the presence of, or following treatment with C-AGP.
Aso
H
H
Cellular Biology Laboratory, National Institute of Animal Industry, Tsukuba, Japan.
Tamura
K
K
Rose
M T
MT
Tomioka
Y
Y
Mizugaki
M
M
Ishida
N
N
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001202:Ascitic Fluid / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007372:Interferons / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D009961:Orosomucoid / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007376:Interleukin-2; D009961:Orosomucoid; D011994:Recombinant Proteins; D007372:Interferons; D007213:Indomethacin