Isolation, purification, crystallization and preliminary X-ray analysis of beta 1-bungarotoxin from Bungarus caeruleus (Indian common krait).
Beta-Bungarotoxin is a heterodimeric neurotoxin consisting of a phospholipase A2 subunit linked by a disulfide bond to a K+ channel binding subunit, which is a member of of the Kunitz protease-inhibitor subfamily. Purified beta1-bungarotoxin was crystallized using microdialysis techniques. The rectangular-shaped crystals are orthorhombic, space group C2221, with unit-cell dimensions a = 80.7, b = 82.5, c = 56.9 A. The crystals have a calculated Vm of 2.35 A3 Da-1 for one molecule in the asymmetric unit. This corresponds to a solvent content of 48%. X-ray intensity data were collected to 2.6 A resolution. The data set is 97.4% complete.
Sharma
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S
Department of Biophysics, All India Institute of Medical Sciences, New Delhi 11 00 29, India.
Karthikeyan
S
S
Betzel
C
C
Singh
T P
TP
D000818:Animals; D002038:Bungarotoxins / Q000737:chemistry* / Q000302:isolation & purification; D017838:Bungarus / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray
D002038:Bungarotoxins