Humoral p53 antibody response is a prognostic parameter in ovarian cancer.
BACKGROUND
Mutant p53 protein may become the target of a tumor-specific humoral and cellular immune response.


METHODS
We used a specific qualitative p53 antibody ELISA to investigate serum samples of 33 patients with ovarian cancer taken prior to therapy. Additionally, we sought to evaluate whether p53 antibodies are also present in the sera of 17 patients with benign ovarian tumors.


RESULTS
p53 antibodies were detected in 36% of serum samples. There was a statistically significant association between p53 serum antibody response and poor overall survival (p < 0.006). No significant associations were found between p53 antibody status and histological type, histological grade, and tumor stage. In 81% of serum samples, no changes from p53 antibody negativity to positivity or vice versa during follow-up were observed. p53 antibodies were also detected in the sera of 18% of patients with benign ovarian tumors.


CONCLUSIONS
The results of this preliminary study suggest that a p53 antibody response in patients with ovarian cancer is associated with poor prognosis. A qualitative method of p53 antibody detection cannot be used to monitor the clinical course of ovarian cancer.
Mayerhofer
K
K
Department of Obstetrics and Gynecology, University of Vienna, Medical School, Austria. klaus.mayerhofer@akh-wien.ac.at
Tempfer
C
C
Kucera
E
E
Hefler
L
L
Zeisler
H
H
Kainz
C
C
Zeillinger
R
R
Sliutz
G
G
D000912:Antibodies, Neoplasm / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000401:mortality; D011237:Predictive Value of Tests; D011379:Prognosis; D012189:Retrospective Studies; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000276:immunology*
D000912:Antibodies, Neoplasm; D016159:Tumor Suppressor Protein p53