Inhibition of Ets-1 DNA binding and ternary complex formation between Ets-1, NF-kappaB, and DNA by a designed DNA-binding ligand.
Sequence-specific pyrrole-imidazole polyamides can be designed to interfere with transcription factor binding and to regulate gene expression, both in vitro and in living cells. Polyamides bound adjacent to the recognition sites for TBP, Ets-1, and LEF-1 in the human immunodeficiency virus, type 1 (HIV-1), long terminal repeat inhibited transcription in cell-free assays and viral replication in human peripheral blood lymphocytes. The DNA binding activity of the transcription factor Ets-1 is specifically inhibited by a polyamide bound in the minor groove. Ets-1 is a member of the winged-helix-turn-helix family of transcription factors and binds DNA through a recognition helix bound in the major groove with additional phosphate contacts on either side of this major groove interaction. The inhibitory polyamide possibly interferes with phosphate contacts made by Ets-1, by occupying the adjacent minor groove. Full-length Ets-1 binds the HIV-1 enhancer through cooperative interactions with the p50 subunit of NF-kappaB, and the Ets-inhibitory polyamide also blocks formation of ternary Ets-1. NF-kappaB.DNA complexes on the HIV-1 enhancer. A polyamide bound adjacent to the recognition site for NF-kappaB also inhibits NF-kappaB binding and ternary complex formation. These results broaden the application range of minor groove-binding polyamides and demonstrate that these DNA ligands are powerful inhibitors of DNA-binding proteins that predominantly use major groove contacts and of cooperative protein-DNA ternary complexes.
Dickinson
L A
LA
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Trauger
J W
JW
Baird
E E
EE
Dervan
P B
PB
Graves
B J
BJ
Gottesfeld
J M
JM
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016322:HIV Enhancer; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D008024:Ligands; D016328:NF-kappa B / Q000378:metabolism*; D009757:Nylons / Q000378:metabolism; D011485:Protein Binding; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C494041:ETS1 protein, human; D008024:Ligands; D016328:NF-kappa B; D009757:Nylons; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; D004247:DNA