Comparison of the electrooptical properties and specific respiratory activity of Acinetobacter calcoaceticum A-122.
The electrooptical (EO) properties of a cell suspension and the specific respiratory activity of cells towards p-nitrophenol (PNP) were compared during PNP metabolism in Acinetobacter calcoaceticum strain A-122. The frequency dependence of the suspension's turbidity changes due to cellular orientation (orientational spectra) at frequencies of an orienting electric field of 10-10,000 kHz was determined. Orientational spectral changes observed during PNP incubation of the cells were followed over the range of 10-502 kHz. There were linear relationships between the magnitude of the EO effect at a 502-kHz frequency and the concentration of PNP over the range of 0.1-0.8 mM, and between the specific respiratory activity of the cells and the concentration of PNP over the range of 0.1-1.0 mM. The knowledge gained from these studies suggests a direct relationship between alterations in the cellular EO properties and PNP metabolism.
Ignatov
O V
OV
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia. oignatov@ibppm.saratov.su
Khorkina
N A
NA
Shchyogolev SYu
Singirtsev
I N
IN
Bunin
V D
VD
Tumaikina
Y A
YA
Ignatov
V V
VV
D016954:Acinetobacter calcoaceticus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D055592:Biophysical Phenomena; D001703:Biophysics; D004563:Electrochemistry; D009596:Nitrophenols / Q000378:metabolism*; D055095:Optics and Photonics; D010101:Oxygen Consumption
D009596:Nitrophenols; C024836:4-nitrophenol