Isolation of cDNA clones encoding two isoforms of nucleoside diphosphate kinase from bovine retina.
The cDNA encoding bovine retinal isoforms of nucleoside diphosphate kinase (NDP-kinase, EC 2.7.4.6) has been cloned and sequenced. Based on the partial amino acid sequence of the enzyme determined after trypsin digestion of purified NDP-kinase, primers were synthesized and used to isolate two different cDNA clones encoding the full length of two NDP-kinase isoforms. The nucleotide sequences of these clones contained open reading frames encoding 152-residue polypeptides with calculated molecular masses of 17.262 and 17.299 kDa, similar to that determined for the subunits of purified enzyme (17.5 and 18.5 kDa). The deduced NDP-kinase sequences showed high similarity with the known NDP-kinase sequences from other sources.
Karaschuk
G
G
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. karas@ibch.siobc.ras.ru
Kakuev
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics*; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D012160:Retina / Q000201:enzymology*
D018076:DNA, Complementary; D007527:Isoenzymes; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D009701:Nucleoside-Diphosphate Kinase