Time course and age dependence of motor neuron death following facial nerve crush injury: role of fibroblast growth factor.
Peripheral nerve crush injury (PNCI) has been used for many years in adult animals to study central and peripheral changes related to regeneration across the injury site. While these adult animals experience full recovery with no neuronal cell loss following PNCI, it has been noted that the injury in perinatal animals is followed by retrograde neuronal cell death. The present study determines, in mice of different postnatal ages, the degree to which motor neurons are vulnerable to PNCI induced cell death and examines the rate of neuronal loss. Animals of 4 days of age and younger were found to be significantly more vulnerable to motor neuron cell death following PNCI. There also was a proportional relationship between age at injury and final motor neuronal survival and an inverse relationship between age at injury and rate of neuronal cell death following injury. In addition a proportional relationship was observed between the expression level of acidic fibroblast growth factor within motor neurons and the resistance to PNCI induced neuronal death. It was also found that PNCI in an environment that contained higher levels of FGFs (either in mice treated with acidic FGF or in transgenic mice that overexpress basic FGF) significantly decreases neuronal cell death following early postnatal injury.
Kuzis
K
K
Department of Cell and Developmental Biology, Oregon Health Sciences University, Portland, Oregon 97201, USA.
Coffin
J D
JD
Eckenstein
F P
FP
D000375:Aging / Q000502:physiology*; D000818:Animals; D016923:Cell Death / Q000502:physiology; D020220:Facial Nerve Injuries; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000627:therapeutic use; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic / Q000235:genetics; D009046:Motor Neurons / Q000502:physiology*; D009409:Nerve Crush; D013997:Time Factors; D014949:Wounds, Nonpenetrating / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors