title: Allelic imbalance in the clonal evolution of prostate carcinoma.
abstract: BACKGROUND
  To understand better the genetic basis of the clonal evolution of prostate carcinoma, the authors analyzed the pattern of allelic loss in 25 matched primary and metastatic prostate tumors.
  
  
  METHODS
  Twenty-five cases were selected from the surgical pathology files of the Mayo Clinic from patients who had undergone radical retropubic prostatectomy and bilateral lymphadenectomy between 1987-1991. All patients had regional lymph node metastases at the time of surgery. DNA samples for the analysis of allelic loss pattern were prepared from primary tumors and matched synchronous lymph node metastases by tissue microdissection. The oligonucleotide primer pairs for the microsatellite DNA markers were D8S133, D8S136, D8S137, ANK1 on chromosome 8p12-21, LPLTET on chromosome 8p22, and D17S855 (intragenic to the BRCA1 gene) on chromosome 17q21. One case was not informative at any of the loci tested and was excluded from further analysis.
  
  
  RESULTS
  The overall frequency of allelic imbalance was 79% in primary tumors and 88% in paired metastases. Of 24 informative cases, 14 patients (58%) showed the same pattern of allelic loss or retention in matched primary and metastatic tumors at all marker locus; discordant allelic loss was observed in the remaining 10 patients (42%). Four patients showed loss of the same allele at one or more marker loci in both primary and metastatic tumors, but discordant allelic loss was observed at other marker loci. Five patients showed allelic loss in at least one genetic marker in the metastatic tumor but not in its matched primary tumor. Five patients displayed loss of one allele at one or more marker loci in a primary tumor but not in the matched metastases. There was no significant difference in the frequency of allelic imbalance between primary and metastatic tumors at any marker analyzed (P>0.05).
  
  
  CONCLUSIONS
  These data suggest that different patterns of allelic deletion may be acquired during cancer progression to metastases. The differences in genetic composition between primary prostate carcinoma and its metastases may be related to intrinsic cancer heterogeneity, overall genetic instability, and clonal divergence.
authors.affiliation: Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis 46202, USA. lcheng@iupui.edu
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: D G
authors.initials: DG
authors.lastname: Bostwick
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Hu
authors.forename: A O
authors.initials: AO
authors.lastname: Vortmeyer
authors.forename: Z
authors.initials: Z
authors.lastname: Zhuang
mesh_terms: D000368:Aged; D000483:Alleles; D002999:Clone Cells / Q000473:pathology; D018450:Disease Progression; D006801:Humans; D008197:Lymph Node Excision; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012189:Retrospective Studies