Neuroendocrine mediators up-regulate alpha1b- and alpha1d-adrenergic receptor subtypes in human monocytes.
Beta2- and alpha2-adrenergic receptors (AR) are thought to be the main AR subtypes to exert the effects of catecholamines on the immune system. However, in the present study, we demonstrate that another subtype of AR can be induced in human monocytes. Expression of alpha1b- and alpha1d-AR mRNA can be obtained by culturing freshly isolated human peripheral blood monocytes with the neuroendocrine mediators dexamethasone or the beta2-AR agonist terbutaline. Using the human monocytic cell line THP-1, we demonstrate that increased levels of alpha1b- and alpha1d-mRNA are accompanied by increased levels of receptor protein as determined by Western blot analysis and radioligand binding assays. This study describes for the first time regulated expression of alpha1-AR subtypes in human monocytes.
Rouppe van der Voort
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Department of Immunology, University Hospital for Children and Youth Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands. c.rouppe@wkz.azu.nl
Kavelaars
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van de Pol
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Heijnen
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CJ
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D015153:Blotting, Western; D002395:Catecholamines / Q000378:metabolism; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013726:Terbutaline / Q000494:pharmacology; D015854:Up-Regulation / Q000276:immunology
C547716:ADRA1B protein, human; C547719:ADRA1D protein, human; D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D005938:Glucocorticoids; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D011943:Receptors, Adrenergic, beta; D003907:Dexamethasone; D013726:Terbutaline