title: Development and validation of a method for the analysis of a pharmaceutical preparation by near-infrared diffuse reflectance spectroscopy.
abstract: A near-infrared (NIR) spectroscopic method based on the use of a fiber optical probe for the analysis of a commercially available pharmaceutical preparation is proposed. The analyte is identified by comparison with a second-derivative spectral library, using the correlation coefficient as the discriminating parameter. Once a sample has been positively identified, the active principle is quantified with partial least-squares (PLS) calibration. The proposed method was validated for use as a control method; to this end, the selectivity of the identification process, and the repeatability, intermediate precision, accuracy, linearity, and robustness of the active principle quantitation, were assessed.
authors.affiliation: Departamento de Química, Unidad de Química Analítica, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain. iqan8@blues.uab.es
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Coello
authors.forename: A
authors.initials: A
authors.lastname: Eustaquio
authors.forename: H
authors.initials: H
authors.lastname: Iturriaga
authors.forename: S
authors.initials: S
authors.lastname: Maspoch
mesh_terms: D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared