[Comparison of changes in optical density of mu-opioid receptors in the brain of adult rats after prenatal and postnatal hypoxia].
The opioid system modulates the growth and development of CNS. Hypoxia increases endogenous opioids significantly. The early development of the opioid receptor system has been reported to be susceptible to perinatal opioid exposure so the developing brain may be influenced by circulating opioids. To test the hypothesis that perinatal hypoxia affects the development of the opioid receptor system in developing brain, we performed the following study. We divided 36 pregnant Sprague-Dawley rats to three treatment groups and control group. We exposed one group (C) to chronic prenatal hypoxia (10.5% O2) continuously 24 h/day and second group (AH) to acute, intermittent hypoxia (9.0% O2) 6 h/day from day E15 to E20. Group of newborns (PH) we exposed to postnatal hypoxia (10.5% O2) from day P2-10. We analyzed the several brain regions: CPu, NA, MMPoA, LMPoA, CA3, VM, AH, Habenula by quantitative autoradiography following incubation with (3H) DAMGO.


RESULTS
1) Chronic prenatal hypoxia decreased mu-receptor binding in all brain regions examined. 2) In contrast, mu-receptor binding following acute, intermittent hypoxia tended to be increased in the several brain regions. 3) Postnatal hypoxia did not change mu-receptor binding in characteristic way. 4) Such receptor changes may be associated with endocrinologic or behavioral abnormalities.
Kraczkowski
J J
JJ
Kliniki Połoznictwa i Patologii Ciazy Akademii Medycznej w Lublinie.
Semczuk
M
M
D000208:Acute Disease; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D001921:Brain / Q000196:embryology* / Q000378:metabolism*; D002452:Cell Count; D002908:Chronic Disease; D005260:Female; D000860:Hypoxia / Q000196:embryology*; D008297:Male; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000196:embryology; D017450:Receptors, Opioid, mu / Q000378:metabolism*
D017450:Receptors, Opioid, mu