title: Leishmania (Viannia) braziliensis is the predominant species infecting patients with American cutaneous leishmaniasis in the State of Minas Gerais, Southeast Brazil.
abstract: Skin biopsies from 53 patients with American cutaneous leishmaniasis (ACL) from the State of Minas Gerais, Brazil, were used for a characterization of the Leishmania parasites. A pair of primers flanking the conserved region of the Leishmania minicircle kDNA was used to obtain amplified DNA via the polymerase chain reaction. The amplified products were subsequently hybridized with Leishmania subgenus-specific radiolabeled probes. Parasites from 49 out of 53 samples (92.5%) were characterized as belonging to the subgenus Viannia and four (7.5%) as belonging to the subgenus Leishmania. Clinical, epidemiological and molecular evidence allow us to conclude that Leishmania (V.) braziliensis and Leishmania (L.) amazonensis are the species present in the patients studied and that L. (V.) braziliensis is the predominant species in the State of Minas Gerais, Brazil.
authors.affiliation: Departamento de Clinica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: V M
authors.initials: VM
authors.lastname: Passos
authors.forename: O
authors.initials: O
authors.lastname: Fernandes
authors.forename: P A
authors.initials: PA
authors.lastname: Lacerda
authors.forename: A C
authors.initials: AC
authors.lastname: Volpini
authors.forename: C M
authors.initials: CM
authors.lastname: Gontijo
authors.forename: W
authors.initials: W
authors.lastname: Degrave
authors.forename: A J
authors.initials: AJ
authors.lastname: Romanha
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002648:Child; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D007892:Leishmania braziliensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012867:Skin / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan