Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence.
Eleven thousand, three hundred and seventy enhancer/promoter trap lines in Arabidopsis were generated via T-DNA transformation utilizing the binary vector pD991 that contains a minimal promoter fused to the uidA reporter gene. Overall 31% of the lines generated exhibit a staining pattern in the inflorescence. Flanking DNA has been cloned from 15 lines exhibiting inflorescence staining patterns by either thermal asymmetric interlaced PCR (TAIL-PCR), inverse PCR (IPCR), or partial library construction. Seeds from these lines are available from the ABRC and NASC Arabidopsis stock centers and DNA pools are available from the ABRC.
Campisi
L
L
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
Yang
Y
Y
Yi
Y
Y
Heilig
E
E
Herman
B
B
Cassista
A J
AJ
Allen
D W
DW
Xiang
H
H
Jack
T
T
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D017343:Genes, Plant; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D017931:DNA Primers; D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA; D005966:Glucuronidase