title: Adherence of Staphylococcus aureus is enhanced by an endogenous secreted protein with broad binding activity.
abstract: A novel mechanism for enhancement of adherence of Staphylococcus aureus to host components is described. A secreted protein, Eap (extracellular adherence protein), was purified from the supernatant of S. aureus Newman and found to be able to bind to at least seven plasma proteins, e.g., fibronectin, the alpha-chain of fibrinogen, and prothrombin, and to the surface of S. aureus. Eap bound much less to cells of Staphylococcus epidermidis, Streptococcus mutans, or Escherichia coli. The protein can form oligomeric forms and is able to cause agglutination of S. aureus. Binding of S. aureus to fibroblasts and epithelial cells was significantly enhanced by addition of Eap, presumably due to its affinity both for plasma proteins on the cells and for the bacteria.
authors.affiliation: Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institutet, Huddinge University Hospital, F82, S-141 86 Huddinge, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Palma
authors.forename: A
authors.initials: A
authors.lastname: Haggar
authors.forename: J I
authors.initials: JI
authors.lastname: Flock
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D001798:Blood Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000302:isolation & purification; D004847:Epithelial Cells / Q000382:microbiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000382:microbiology; D011485:Protein Binding; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D001798:Blood Proteins; D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; C095564:Map protein, Staphylococcus aureus