title: Identification of squamous cell carcinoma antigen-2 in tumor tissue by two-dimensional electrophoresis.
abstract: The aim of this study was to identify two homologous serine proteinase inhibitor (serpin) molecules, squamous cell carcinoma (SCC) antigen-1 and -2, by two-dimensional electrophoresis (2-DE), combined with immunoblotting, and examine their expression in tumor tissue. The recombinant SCC (rSCC) antigen-1 showed four spots with p/ 6.5, 6.4, 6.3 and 6.0, whereas rSCC antigen-2 showed a more acidic spot with p/5.95. SCC antigen in tumor tissue appeared in three new acidic spots (p/5.7-5.5, M(r) 44 500), numbered 5, 6 and 7, besides the previously reported four spots numbered 1 to 4. These new acidic spots of SCC antigen apparently increased in SCC tissue. Treatment of tissue extract by carboxymethyl (CM)-papain agarose matrix extinguished spots 1 to 4 encoded on the SCCA1 gene, but not 5 to 7 on the SCCA2 gene. Overexpression of the SCCA2 gene may play an important role in the malignant behavior of tumor cells.
authors.affiliation: Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Ube, Japan. obgyn@po.cc.yamaguchi-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Nawata
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Hirabayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: H
authors.initials: H
authors.lastname: Ogata
authors.forename: Y
authors.initials: Y
authors.lastname: Suminami
authors.forename: F
authors.initials: F
authors.lastname: Numa
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Kato
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D010206:Papain; D011994:Recombinant Proteins / Q000032:analysis; D012685:Sepharose; D015843:Serpins
chemical_list: D000951:Antigens, Neoplasm; D011994:Recombinant Proteins; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D012685:Sepharose; D010206:Papain