Neuronal messengers and peptide receptors in the human sphenopalatine and otic ganglia.
A majority of the parasympathetic nerve fibers to cranial structures derive from the sphenopalatine and otic ganglia. In particular, blood vessels are invested with a rich supply of dilator fibers of parasympathetic origin. In the present study, we have examined the occurrence of noncholinergic neuromessengers and neuropeptide receptors in the human sphenopalatine and otic ganglia. Vasoactive intestinal peptide (VIP)-immunoreactive (ir) nerve cell bodies occurred in high numbers in the sphenopalatine and otic ganglia. Likewise, high numbers of NOS- and PACAP-containing nerve cell bodies were seen in both ganglia. Autofluorescent lipofuscin, characteristic of adult human nervous tissue, was present within many nerve cell bodies in both ganglia. Receptor mRNA was studied with reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNA from the sphenopalatine and otic ganglia was successfully extracted. By using appropriate sense and antisense primers, oligonucleotides were designed from the human sequences derived from GenBank, corresponding to human NPY Y1, CGRP1 and VIP1 receptors. In the sphenopalatine ganglion, we revealed the presence of mRNA for the human NPY Y1 and VIP1 receptors but not the CGRP1 receptor. The otic ganglion was found to react positively only for primers to mRNA for VIP1 but not for CGRP1 or NPY Y1 receptors.
Uddman
R
R
Department of Otorhinolaryngology, Malmö University Hospital, S-20502, Malmö, Sweden.
Tajti
J
J
Möller
S
S
Sundler
F
F
Edvinsson
L
L
D000368:Aged; D000369:Aged, 80 and over; D001306:Auditory Pathways / Q000737:chemistry / Q000502:physiology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000235:genetics; D017931:DNA Primers; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005726:Ganglia, Parasympathetic / Q000737:chemistry* / Q000502:physiology; D017950:Ganglia, Sensory / Q000737:chemistry / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology; D009478:Neuropeptide Y / Q000032:analysis / Q000235:genetics; D009479:Neuropeptides / Q000032:analysis / Q000235:genetics*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000235:genetics; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger / Q000032:analysis; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000032:analysis / Q000235:genetics; D018013:Receptors, Neuropeptide / Q000032:analysis / Q000235:genetics*; D017476:Receptors, Neuropeptide Y / Q000032:analysis / Q000235:genetics; D018005:Receptors, Vasoactive Intestinal Peptide / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014660:Vasoactive Intestinal Peptide / Q000032:analysis / Q000235:genetics
C495401:ADCYAP1 protein, human; D017931:DNA Primers; D009478:Neuropeptide Y; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger; D018015:Receptors, Calcitonin Gene-Related Peptide; D018013:Receptors, Neuropeptide; D017476:Receptors, Neuropeptide Y; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase; D015740:Calcitonin Gene-Related Peptide