title: Pseudomonas aeruginosa alginate is a potent secretagogue in the isolated ferret trachea.
abstract: Airway mucus hypersecretion is in part a response to infection and inflammation. Pseudomonas aeruginosa infection is nearly universal in advanced cystic fibrosis (CF) lung disease. Mucoid strains of P. aeruginosa produce an exopolysaccharide product called alginate. The purpose of this study was to determine whether P. aeruginosa alginate stimulates secretion from mucous or serous cells in the ferret trachea exposed to alginate at concentrations reported to be present in the CF airway. We used a sandwich enzyme-linked lectin assay (ELLA) to measure mucin secretion and spectrophotometry to measure lysozyme secretion from isolated ferret tracheal segments. Purified Pseudomonas aeruginosa alginate stimulated mucin and lysozyme secretion in a dose-dependent fashion (mucin = +111%: P = 0.003; lysozyme = +20%: P = 0.024 at 200 microg/mL). This stimulated secretion was not due to proteolytic activity, and alginate exposure did not produce ultrastructural damage to the trachea. We conclude that alginate may contribute to mucus hypersecretion and respiratory morbidity associated with P. aeruginosa infection in patients with CF.
authors.affiliation: Mie University School of Medicine, Tsu, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Kishioka
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: D J
authors.initials: DJ
authors.lastname: Hassett
authors.forename: D
authors.initials: D
authors.lastname: de Mello
authors.forename: B K
authors.initials: BK
authors.lastname: Rubin
mesh_terms: D000464:Alginates / Q000008:administration & dosage / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005289:Ferrets; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009113:Muramidase / Q000187:drug effects / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014132:Trachea / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology* / Q000648:ultrastructure
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D037102:Lectins; D009077:Mucins; D020723:Glucuronic Acid; D009113:Muramidase