Cloning and functional activity of a novel truncated form of annexin IV in mouse macrophages.
Annexin IV was cloned and sequenced from a mouse bone marrow-derived macrophage cDNA library, and was found to exist as three different alternatively spliced transcripts. One transcript contained an additional 688 base pairs inserted within the coding region of the gene including an in-frame stop codon. Translation of this transcript in vitro confirmed the premature arrest of translation which resulted in a truncated annexin IV protein of approximately 22 kDa. Like other members of the annexin family, the product of the wild-type annexin IV transcript bound in a calcium-dependent manner to both phenyl-sepharose and phospholipid vesicles. In contrast, the truncated annexin IV product bound to these substrates in a Ca2+-independent fashion. The existence of a novel form of annexin IV in mouse macrophages may aid in further defining the role of members of the annexin family.
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Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado, 80206, USA.
Riches
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DW
D000595:Amino Acid Sequence; D000818:Animals; D017314:Annexin A4 / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D012685:Sepharose / Q000031:analogs & derivatives / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D017314:Annexin A4; D018076:DNA, Complementary; D010743:Phospholipids; C020927:phenyl-sepharose; D012685:Sepharose; D002118:Calcium