Catalytic DNA- and RNA-hydrolyzing antibodies from milk of healthy human mothers.
Various catalytically active antibodies (Abs), or abzymes, have been detected recently in the sera of patients with autoimmune pathologies, in whom their presence is probably associated with autoimmunization. Normal humans are generally not considered to have abzymes, since no obvious immunizing factors are present. Here is shown by different methods that IgG from the milk of normal females possesses both DNase and RNase activities. The activities were also present in the IgG F(ab')2 and Fab fragments. Affinity modification of IgG by the chemically reactive derivative of an oligonucleotide led to preferential modification of the L chain of IgG. After separation of the subunits by sodium dodecyl sulfate electrophoresis in a gel containing DNA, an in-gel assay showed DNase activity in the L chain. The L chain separated by affinity chromatography on DNA-cellulose was catalytically active. These findings speak in favor of the generation of catalytic Abs by the immune system of healthy mothers. It is known that the treatment of adults with DNases and RNases offers protection from viral and bacterial diseases. Since breast milk protects the infants from infections until the immune system is developed, this raises the possibility that catalytic Abs like nucleases, may possess a protective role.
Buneva
V N
VN
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Russia.
Kanyshkova
T G
TG
Vlassov
A V
AV
Semenov
D V
DV
Khlimankov DYu
Breusova
L R
LR
Nevinsky
G A
GA
D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000276:immunology; D017151:Antibodies, Catalytic / Q000032:analysis* / Q000276:immunology; D001483:Base Sequence; D004247:DNA / Q000276:immunology / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D066298:In Vitro Techniques; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000276:immunology / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
D000974:Antibodies, Antinuclear; D017151:Antibodies, Catalytic; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases