title: Mutational analysis of the role of HPr in Listeria monocytogenes.
abstract: The regulatory role of HPr, a protein of the phosphotransferase system (PTS), was investigated in Listeria monocytogenes. By constructing mutations in the conserved histidine 15 and serine 46 residues of HPr, we were able to examine how HPr regulates PTS activity. The results indicated that histidine 15 was phosphorylated in a phosphoenolpyruvate (PEP)-dependent manner and was essential for PTS activity. Serine 46 was phosphorylated in an ATP-dependent manner by a membrane-associated kinase. ATP-dependent phosphorylation of serine 46 was significantly enhanced in the presence of fructose 1,6-diphosphate and resulted in a reduction of PTS activity. The presence of a charge at position 15 did not inhibit ATP-dependent phosphorylation of serine 46, a finding unique to gram-positive PEP-dependent PTSs studied to this point. Finally, HPr phosphorylated at serine 46 does not appear to possess self-phosphatase activity, suggesting a specific phosphatase protein may be essential for the recycling of HPr to its active form.
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0919, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Christensen
authors.forename: A K
authors.initials: AK
authors.lastname: Benson
authors.forename: R W
authors.initials: RW
authors.lastname: Hutkins
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005635:Fructosediphosphates / Q000494:pharmacology; D015870:Gene Expression; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D017354:Point Mutation; D012694:Serine / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D005635:Fructosediphosphates; D012694:Serine; D000255:Adenosine Triphosphate; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; C029063:fructose-1,6-diphosphate