title: Detection of avian adenovirus by polymerase chain reaction.
abstract: An avian adenovirus-specific polymerase chain reaction was developed. The origin of primers was from the DNA sequence data of the chicken embryo lethal orphan avian adenovirus virus genome. An avian adenovirus-specific 421-bp DNA product was amplified by these primers from group I of adenovirus containing 12 serotypes and serotypes of adenovirus from group II and group III. The adenovirus-specific DNA product was also amplified from the 19 field isolates of avian adenoviruses but not from the mammalian adenovirus and other avian pathogenic viruses and bacteria. As little as 1 fg of avian adenovirus DNA was detected by gel electrophoresis and Southern blot analysis.
authors.affiliation: Department of Pathobiology, College of Agriculture and Natural Resources, University of Connecticut, Storrs 06269-3089, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Xie
authors.forename: A A
authors.initials: AA
authors.lastname: Fadl
authors.forename: T
authors.initials: T
authors.lastname: Girshick
authors.forename: M I
authors.initials: MI
authors.lastname: Khan
mesh_terms: D000818:Animals; D000259:Aviadenovirus / Q000302:isolation & purification*; D002642:Chick Embryo; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral