DNA levels in immune complexes circulating in mice with induced systemic lupus erythematosus.
The levels of DNA in IgG immune complexes, which appeared in the circulation of mice after the induction of systemic lupus erythematosus (SLE), were measured by an immunochemical quantitative assay using monoclonal anti-dsDNA antibodies. The amount of DNA in immune complexes was already high at 10-12 days following the injection of a human monoclonal anti-DNA antibody bearing the major idiotype designated 16/6 in complete Freund's adjuvant, i.e. long before the appearance of clinical manifestations. The injections of these antibodies in the alum-precipitated form did not induce the formation of DNA:anti-DNA complexes as well as SLE itself. The levels of DNA in circulating immune complexes were in general high throughout the whole experimental period (up to 7 months) decreasing gradually before the first clinical manifestations appeared and thereafter, when the disease was fully developed. Such a decrease could be explained by the retention of immune complexes in kidneys. The levels of DNA in immune complexes circulating in normal mice or in mice receiving injections of complete Freund's adjuvant was very low. Treatment of experimental SLE that affected the clinical manifestations prevented the formation of high levels of DNA containing immune complexes.
Nezlin
R
R
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel. linezlin@weizmann.weizmann.ac.il
Dayan
M
M
Zinger
H
H
Mozes
E
E
D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood*; D004247:DNA / Q000097:blood*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D008727:Methotrexate / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D019384:Nucleic Acid Synthesis Inhibitors / Q000627:therapeutic use; D013629:Tamoxifen / Q000627:therapeutic use
D000936:Antigen-Antibody Complex; D019384:Nucleic Acid Synthesis Inhibitors; D013629:Tamoxifen; D004247:DNA; D008727:Methotrexate