title: Effect of factor XIII on endothelial barrier function.
abstract: The effect of factor XIII on endothelial barrier function was studied in a model of cultured monolayers of porcine aortic endothelial cells and saline-perfused rat hearts. The thrombin-activated plasma factor XIII (1 U/ml) reduced albumin permeability of endothelial monolayers within 20 min by 30 +/- 7% (basal value of 5.9 +/- 0.4 x 10(-6) cm/s), whereas the nonactivated plasma factor XIII had no effect. Reduction of permeability to the same extent, i.e., by 34 +/- 9% could be obtained with the thrombin-activated A subunit of factor XIII (1 U/ml), whereas the iodoacetamide-inactivated A subunit as well as the B subunit had no effect on permeability. Endothelial monolayers exposed to the activated factor XIII A exhibited immunoreactive deposition of itself at interfaces of adjacent cells; however, these were not found on exposure to nonactivated factor XIII A or factor XIII B. Hyperpermeability induced by metabolic inhibition (1 mM potassium cyanide plus 1 mM 2-deoxy-D-glucose) was prevented in the presence of the activated factor XIII A. Likewise, the increase in myocardial water content in ischemic-reperfused rat hearts was prevented in its presence. This study shows that activated factor XIII reduces endothelial permeability. It can prevent the loss of endothelial barrier function under conditions of energy depletion. Its effect seems related to a modification of the paracellular passageways in endothelial monolayers.
authors.affiliation: Physiologisches Institut, Justus-Liebig-Universität, D-35392 Giessen, Germany. thomas.noll@physiologie.med.uni-giessen.de
authors.forename: T
authors.initials: T
authors.lastname: Noll
authors.forename: G
authors.initials: G
authors.lastname: Wozniak
authors.forename: K
authors.initials: K
authors.lastname: McCarson
authors.forename: A
authors.initials: A
authors.lastname: Hajimohammad
authors.forename: H J
authors.initials: HJ
authors.lastname: Metzner
authors.forename: J
authors.initials: J
authors.lastname: Inserte
authors.forename: W
authors.initials: W
authors.lastname: Kummer
authors.forename: F W
authors.initials: FW
authors.lastname: Hehrlein
authors.forename: H M
authors.initials: HM
authors.lastname: Piper
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001834:Body Water; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005176:Factor XIII / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013552:Swine
chemical_list: D005176:Factor XIII