title: Diffusely adhering Escherichia coli (DAEC) strains of fecal origin rarely express F1845 adhesin.
abstract: A total of 398 diffusely adhering Escherichia coli (DAEC) strains of fecal origin were analyzed for the presence of sequences homologous to the structural subunit gene (daaE) of the F1845 fimbria. For that purpose, a DNA fragment homologous to daaE, obtained by PCR, was used as a probe in colony hybridization assays. Only two strains carried daaE and expressed F1845, suggesting that this fimbria is rare among DAEC strains.
authors.affiliation: Departamento de Bacteriologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil. lccampos@gene.dbbm.fiocruz.br
authors.forename: L C
authors.initials: LC
authors.lastname: Campos
authors.forename: M A
authors.initials: MA
authors.lastname: Vieira
authors.forename: L R
authors.initials: LR
authors.lastname: Trabulsi
authors.forename: L A
authors.initials: LA
authors.lastname: da Silva
authors.forename: V
authors.initials: V
authors.lastname: Monteiro-Neto
authors.forename: T A
authors.initials: TA
authors.lastname: Gomes
mesh_terms: D018830:Adhesins, Escherichia coli / Q000096:biosynthesis / Q000235:genetics*; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D036781:Fimbriae Proteins; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007223:Infant
chemical_list: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C080952:DaaE protein, E coli; D036781:Fimbriae Proteins