Evaluation of solid phase radiolabels of dog food for gastric emptying.
99mTc-Pertechnetate, 99mTc-mebrofenin, 99mTc-disofenin, 99mTc-sulfur colloid, and 99mTc-Dowex resin beads were evaluated for in vitro stability as a label for both dry extruded, and canned dog food for gastric emptying scintigraphy. A sample of each radiolabeled diet was added to water, gastric juice, intestinal juice, or gastric juice followed by intestinal juice for in vitro digestion. After a 3-hour digestion period, tubes were centrifuged and percentage solid phase retention (%SPR) was calculated. The experiment was repeated three times over a 14-day period to minimize day-to-day variation. For dry dog food, 99mTc-mebrofenin and 99mTc-disofenin had similar %SPR in water (96 and 93%, respectively) and gastric juice (>95% each) and were significantly higher than other labels. For canned dog food, mebrofenin had a 91% SPR for the water or gastric juice digestions, and 99mTc-Dowex had a %SPR of >99%. 99mTc-mebrofenin and 99mTc-Dowex were also tested in vivo, where 99mTc-Dowex had poor stability, and 99mTc-Mebrofenin had excellent stability. 99mTc-Mebrofenin is a suitable label for dog food for gastric emptying scintigraphy.
Kunze
C P
CP
Department of Clinical Sciences, Kansas State University, Manhattan 66506, USA.
Hoskinson
J J
JJ
Butine
M D
MD
Goggin
J M
JM
D000704:Analysis of Variance; D000821:Animal Feed; D000818:Animals; D002855:Chromatography, Thin Layer; D004063:Digestion; D004064:Digestive System / Q000000981:diagnostic imaging*; D004068:Digestive System Physiological Phenomena; D004285:Dogs; D005069:Evaluation Studies as Topic; D005746:Gastric Emptying; D005750:Gastric Juice; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D017556:Technetium Compounds
D019275:Radiopharmaceuticals; D017556:Technetium Compounds