A specific PCR to differentiate between gE negative vaccine and wildtype bovine herpesvirus type 1 strains.
In the context of infectious bovine rhinotracheitis (IBR) control programmes using glycoprotein E (gE) deleted marker vaccines, a PCR assay was developed to allow the genotypic differentiation between wildtype bovine herpesvirus type 1 (BoHV-1) and gE negative strains. This assay is based on the PCR amplification of a 281 bp DNA fragment within the gE gene. The specificity of the amplification was confirmed by restriction endonuclease analysis and nucleotide sequencing of the PCR product. Its ability to determine the gE genotype of BoHV-1 strains was demonstrated on isolates coming from 20 experimental calves infected with four different BoHV-1 strains. This PCR assay may be a useful tool for monitoring the spread of live marker vaccine and the gE genotype of viral field isolates.
Schynts
F
F
Virology Department, Faculty of Veterinary Medicine, University of Liège, Belgium.
Baranowski
E
E
Lemaire
M
M
Thiry
E
E
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007241:Infectious Bovine Rhinotracheitis / Q000517:prevention & control / Q000821:virology*; D007668:Kidney; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013737:Testis; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D017931:DNA Primers; D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific