Dehydrogenase activity of infected cells and biological properties of HIV-1 variants.
Changes in dehydrogenase activity of some continuous cell lines were studied during the response to acute infection in vitro with HIV-1 variants having different biological features. Soon after infection, the dehydrogenase activity of infected cells increased, and this increase was greater and more prolonged with the HIV-1 r/h variant than with the HIV-1 s/l variant. Later stages showed decreased dehydrogenase activity of HIV-1-infected cells compared to the noninfected control; this is a manifestation of the cytodestructive effect of the virus. The changes increased monotonously (but not in direct proportion) with an increase in the infecting dose.
Shchelkanov
M Y
MY
Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow, 123098, Russia.
Eremin
V F
VF
Sakhuriya
I B
IB
Burunova
V V
VV
Pavlova
T V
TV
Kornilaeva
G V
GV
Karamov
E V
EV
D002460:Cell Line; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000097:blood / Q000201:enzymology; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000821:virology; D010088:Oxidoreductases / Q000097:blood*
D016655:HIV Core Protein p24; D010088:Oxidoreductases; D054303:HIV Reverse Transcriptase