Development of the GABA system in organotypic culture of hippocampal and cerebellar slices from a 67-kDa isoform of glutamic acid decarboxylase (GAD67)-deficient mice.
Mice lacking glutamic acid decarboxylase 67kDa (GAD67) are born with much reduced GABA contents but do not survive after birth. To estimate the subsequent development of GAD67-deficient neural tissue, the hippocampal and cerebellar slices were prepared while the mice were alive, and subjected to organotypic culture. Although GABA contents were initially less than 5%, they were markedly increased, and GABA-containing fiber networks were well developed during the culture, indicating that GAD65, the other GAD isoform, largely compensates for the lack of GAD67. Cerebellar Purkinje cells developed similarly in culture.
Ji
F
F
Laboratory of Neurochemistry, National Institute for Physiological Sciences and School of Life Sciences, Graduate University for Advanced Studies, Okazaki, Japan.
Obata
K
K
D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009924:Organ Culture Techniques; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D007527:Isoenzymes; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase