title: Alcohol-induced pancreatic oxidative stress: protection by phospholipid repletion.
abstract: Oxidative stress is considered to be a forerunner of pancreatitis. Since we had found polyenylphosphatidylcholine, a mixture of polyunsaturated phosphatidylcholines extracted from soybeans, to protect against hepatic oxidative stress, we now tested its effects on the pancreas. Sprague-Dawley rats were pair-fed for two months nutritionally adequate liquid diet containing ethanol (36% of energy) or isocaloric carbohydrate, with either polyenylphosphatidylcholine (3 g/1000 kcal) or safflower oil, with or without 5 g/1000 kcal carbonyl iron. Parameters of oxidative stress (F2-isoprostanes, 4-hydroxynonenal, reduced glutathione), ubiquinol-10, ubiquinol-9 and vitamin E, as well as phosphatidylcholine species, were assessed by GC/MS and/or HPLC. Alcohol feeding increased pancreatic 4-hydroxynonenal three-fold, F2-isoprostanes and ubiquinol-9 by more than 70%, whereas it decreased total phospholipids, several phosphatidylcholine species, ubiquinol-10 and glutathione, especially in iron fed rats. Polyenylphosphatidylcholine prevented the rise in 4-hydroxynonenal and F2-isoprostanes, the decrease in dilinoleoylphosphatidylcholine and oleoyllinoleoylphosphatidylcholine and opposed the alcohol-induced decrease of glutathione; alpha-tocopherol remained unchanged. Iron had no significant effect except for decreasing ubiquinol-10 in the pancreas and increasing aminotransferases in the plasma. Thus, the alcohol-induced oxidative stress in the pancreas was shown to be prevented by polyenylphosphatidylcholine which may act, in part, by correcting the depletion of several phosphatidylcholine species.
authors.affiliation: Section of Liver Disease & Nutrition and Alcohol Research Center, Bronx VA Medical Center and Mt. Sinai School of Medicine, New York, NY 10468, USA.
authors.forename: S I
authors.initials: SI
authors.lastname: Aleynik
authors.forename: M A
authors.initials: MA
authors.lastname: Leo
authors.forename: M K
authors.initials: MK
authors.lastname: Aleynik
authors.forename: C S
authors.initials: CS
authors.lastname: Lieber
mesh_terms: D000437:Alcoholism / Q000503:physiopathology*; D000447:Aldehydes / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D019587:Dietary Supplements; D015237:Dinoprost / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D019803:Glutathione Disulfide / Q000032:analysis; D007501:Iron / Q000008:administration & dosage / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012450:Safflower Oil / Q000008:administration & dosage / Q000494:pharmacology*; D013025:Glycine max
chemical_list: D000447:Aldehydes; D010713:Phosphatidylcholines; C005152:lipostabil; D000431:Ethanol; D012450:Safflower Oil; D015237:Dinoprost; D007501:Iron; C027576:4-hydroxy-2-nonenal; D019803:Glutathione Disulfide