A novel mouse gene, HemT, encoding an hematopoietic cell-specific transcript.
We performed differential display to identify hematopoietic cell-specific genes that are expressed differentially before and after mouse erythroleukemia cell (MELC) induction. In this study we report the identification, cloning, and characterization of one such mouse gene, HemT. Using Northern blot, RT-PCR and in situ hybridization, we show that HemT encodes three different transcripts. One of them, HemT-1, is hematopoietic-cell specific and may be erythroid cell-specific. Another, HemT-2, is expressed mainly in hematopoietic cells. HemT-1 and HemT-2 are alternative splicing products. A third type of HemT transcript is expressed only in testis. The erythroid transcript is expressed preferentially in uninduced MELC as compared to induced MELC, and is likely to be expressed in early erythroid cell populations.
Xue
H
H
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
O'Neill
D
D
Morrow
J
J
Bank
A
A
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D001854:Bone Marrow Cells / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D018076:DNA, Complementary; C119617:HemT protein, mouse; D012333:RNA, Messenger