Determination of trans-resveratrol in human plasma by high-performance liquid chromatography.
The first method using high-performance liquid chromatography (HPLC) has been developed for the determination of trans-resveratrol in human plasma. The method involves a liquid--liquid extraction followed by reversed-phase HPLC with UV detection. The detection limit of trans-resveratrol in human plasma was 5.0 ng/ml. Standard curves are linear over the concentration range of 5.0-5000.0 ng/ml. Intra-assay variability ranged from 1.9 to 3.7% and inter-assay variability ranged from 2.5 to 4.0% at the concentration range of 15.0-4000.0 ng/ml.
Zhu
Z
Z
Pharmascience, Montreal, Quebec, Canada. zzhu@pharmascience.com
Klironomos
G
G
Vachereau
A
A
Neirinck
L
L
Goodman
D W
DW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013267:Stilbenes / Q000097:blood*
D013267:Stilbenes; D000077185:Resveratrol