title: Growth and hydrolytic enzyme production of Capnocytophaga gingivalis on different protein substrates.
abstract: Capnocytophaga gingivalis was grown with proteins (albumin, collagen, mucin and hemoglobin) as carbon and energy sources in chemostat culture. The mu max (0.34 h-1) and biomass yield (0.96 g.l-1) were as high with hemoglobin (3 g.l-1) as with glucose (3 g.l-1) (20). Albumin, collagen and mucin also supported an increased mu max, or yield or both, in comparison with basal (tryptone/thiamine) medium. In steady-state, trypsin-like protease specific activity increased 3- to 5-fold in the presence of albumin, collagen and hemoglobin: whereas the greatest increase (21-fold) in alpha-glucoside activity was in the presence of mucin. There were significant, but less substantial changes in other hydrolytic enzymes (aminopeptidase, acid and alkaline phosphatases). The bulk of the detected hydrolytic activity (> 66%) was associated with the cells. The data indicate that C. gingivalis regulates its production of hydrolytic enzymes in response to environmental conditions.
authors.affiliation: Faculty of Applied Sciences, University of the West of England, Bristol, United Kingdom.
authors.forename: D A
authors.initials: DA
authors.lastname: Spratt
authors.forename: J
authors.initials: J
authors.lastname: Greenman
authors.forename: A G
authors.initials: AG
authors.lastname: Schaffer
mesh_terms: D000135:Acid Phosphatase / Q000096:biosynthesis; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000626:Aminopeptidases / Q000096:biosynthesis; D000704:Analysis of Variance; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002206:Capnocytophaga / Q000201:enzymology* / Q000254:growth & development*; D003470:Culture Media; D005959:Glucosidases / Q000096:biosynthesis; D006867:Hydrolases / Q000096:biosynthesis*; D012697:Serine Endopeptidases / Q000096:biosynthesis; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D006867:Hydrolases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005959:Glucosidases; D000626:Aminopeptidases; D012697:Serine Endopeptidases