title: Low-dose whole-body irradiation induces alteration of protein expression in mouse splenocytes.
abstract: In previous studies, the stimulatory effect on immunological function and adaptive response in splenocytes were found after exposure of mice to low-dose whole-body irradiation (LD-WBI). Protein synthesis was found to be required for these responses. The present study, therefore, attempts to investigate the protein changes in mouse splenocytes after exposure to LD-WBI. Two-dimensional gel electrophoresis was used to show the alterations of proteins in nuclei, cytoplasm and extracellular fluid of the splenocytes at 4 h after mice exposed to 75 mGy X-rays. As compared to control, changed expressions of 20, 15 and 6 proteins, in sizes ranging from 10 to 69 kDa with pI of 5.0-8.2, were found in the nuclei, cytoplasm and extracellular fluid of splenocytes, respectively. One protein, shown in the cytoplasm of splenocytes of control mice, appeared in extracellular fluid of splenocytes after LD-WBI. Two proteins, shown in cytoplasm, and one protein, shown in extracellular fluid of splenocytes in control mice, appeared in the nuclei after LD-WBI. Time-course of protein synthesis varied in different proteins after LD-WBI. These results suggest that alterations of protein expressions and redistribution of proteins between intracellular and extracellular compartments occurred in the splenocytes after LD-WBI. Protein extract from LD-WBI splenocytes was fractionated by Sephadex G-100. Fractions 61-80 contained proteins able to protect lymphocytes in vitro from radiation-induced chromosome aberrations. These findings are of importance in elucidating mechanisms of immuno-enhancement and adaptive response induced by low-dose radiation. although the features and the functions of these proteins remain to be elucidated in future studies.
authors.affiliation: Institute of Radiation Medicine, Norman Bethune University of Medical Sciences, Changchun, People's Republic of China.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Cai
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D014916:Whole-Body Irradiation; D014965:X-Rays
chemical_list: D011506:Proteins; D011837:Radiation-Protective Agents