CD4- and CD56-positive T-cell line, MTA, established from natural killer-like T-cell leukemia/lymphoma.
We have established a T-cell line, MTA, from the peripheral blasts of a patient with natural killer (NK)-like T-cell leukemia/lymphoma. The MTA cell displays a T-cell and NK-cell phenotype (CD2+, CD3+, CD4+, CD56+) identical to freshly isolated leukemic blasts from the patient. This cell line shows clonal T-cell receptor rearrangement and a distinguishable character by light microscopy with May-Grünwald Giemsa staining. G-banding analysis showed that the MTA cells had a karyotype of 94(4N), XXXX, add (1) (p36), del (5) (q14q23), add (17) (p11), add (19) (q13). However, unlike NK malignancy, we could not show a direct pathogenic role for Epstein-Barr virus (EBV) with EBV-encoded small RNA and polymerase chain reaction analysis. The MTA cell line is a novel cell line with which to study NK-like T-cell ontogenecity.
Emi
N
N
First Department of Internal Medicine, Nagoya University School of Medicine, Japan. j45973a@nucc.cc.nagoya-u.ac.jp
Abe
A
A
Kasai
M
M
Kohno
A
A
Tanimoto
M
M
Kimura
H
H
Kawashima
K
K
Ito
M
M
Mori
N
N
Saito
H
H
D015704:CD4 Antigens; D019002:CD56 Antigen; D002869:Chromosome Aberrations; D003582:Cytogenetics; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000276:immunology / Q000821:virology*; D008875:Middle Aged; D010641:Phenotype; D014407:Tumor Cells, Cultured
D015704:CD4 Antigens; D019002:CD56 Antigen; D016207:Cytokines