[Plasma concentrations of vitamin K1 and acenocoumarol related to international normalized ratios].
Reduced vitamins K are acting as cofactors of glutamic carboxylation of procoagulant factors (II, VII, IX and X). The reduction of these vitamins is inhibited by oral anticoagulants. The response to antivitamins K is individual and may change during the treatment of a patient. The determinations of plasmatic vitamin K and acenocoumarol may help to explain the mechanism of a resistance to the anticoagulant therapy. Quantifications of vitamin K1 are realized after liquid-liquid extraction with liquid chromatography and fluorescence detection after post-column reduction. For acenocoumarol, plasma concentration is measured, after liquid-liquid extraction, by liquid chromatograph, with ion trap mass spectrometer detector.
Gougnard
T
T
Service de Toxicologie Clinique, Centre Hospitalier Universitaire de Liège.
Charlier
C
C
David
J L
JL
Plomteux
G
G
D000074:Acenocoumarol / Q000097:blood*; D000328:Adult; D000368:Aged; D000925:Anticoagulants / Q000097:blood*; D000933:Antifibrinolytic Agents / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D019934:International Normalized Ratio / Q000379:methods*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D010837:Vitamin K 1 / Q000097:blood*
D000925:Anticoagulants; D000933:Antifibrinolytic Agents; D010837:Vitamin K 1; D000074:Acenocoumarol