title: Isolation, characterization, molecular cloning and molecular modelling of two lectins of different specificities from bluebell (Scilla campanulata) bulbs.
abstract: Two lectins have been isolated from bluebell (Scilla campanulata) bulbs. From their isolation by affinity chromatography, they are characterized as a mannose-binding lectin (SCAman) and a fetuin-binding lectin (SCAfet). SCAman preferentially binds oligosaccharides with alpha(1,3)- and alpha(1,6)-linked mannopyranosides. It is a tetramer of four identical protomers of approx. 13 kDa containing 119 amino acid residues; it is not glycosylated. The fetuin-binding lectin (SCAfet), which is not inhibited by any simple sugars, is also unglycosylated. It is a tetramer of four identical subunits of approx. 28 kDa containing 244 residues. Each 28 kDa subunit is composed of two 14 kDa domains. Both lectins have been cloned from a cDNA library and sequenced. X-ray crystallographic analysis and molecular modelling studies have demonstrated close relationships in sequence and structure between these lectins and other monocot mannose-binding lectins. A refined model of the molecular evolution of the monocot mannose-binding lectins is proposed.
authors.affiliation: School of Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK.
authors.forename: L M
authors.initials: LM
authors.lastname: Wright
authors.forename: E J
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: A
authors.initials: A
authors.lastname: Barre
authors.forename: A K
authors.initials: AK
authors.lastname: Allen
authors.forename: F
authors.initials: F
authors.lastname: Van Leuven
authors.forename: C D
authors.initials: CD
authors.lastname: Reynolds
authors.forename: P
authors.initials: P
authors.lastname: Rouge
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D037222:Collectins; D019143:Evolution, Molecular; D006031:Glycosylation; D006860:Hydrogen Bonding; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D037222:Collectins; D037102:Lectins; D037121:Plant Lectins; D000509:alpha-Fetoproteins; D008358:Mannose