title: Autodegradation of protein disulfide isomerase.
abstract: Protein disulfide isomerase (PDI) and its degradation products were found in HepG2, COS-1, and CHO-K1 cells. Whether or not the products were formed through autodegradation of PDI was examined, since PDI contains the CGHC motif, which is the active center of proteolytic activity in ER-60 protease. Commercial bovine PDI was autodegraded to produce a trimmed PDI. In addition, human recombinant PDI also had autodegradation activity. Mutant recombinant PDIs with CGHC motifs of which cysteine residues were replaced with serine or alanine residues were prepared. However, they were not autodegraded, suggesting the cysteine residues of motifs are necessary for autodegradation.
authors.affiliation: Research Institute for Food Science, Kyoto University, Japan. urade@soya.food.kyoto-u.ac.jp
authors.forename: R
authors.initials: R
authors.lastname: Urade
authors.forename: A
authors.initials: A
authors.lastname: Yasunishi
authors.forename: H
authors.initials: H
authors.lastname: Okudo
authors.forename: T
authors.initials: T
authors.lastname: Moriyama
authors.forename: M
authors.initials: M
authors.lastname: Kito
mesh_terms: D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D018871:Endoplasmic Reticulum, Smooth / Q000201:enzymology; D006801:Humans; D051379:Mice; D016297:Mutagenesis, Site-Directed; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D019704:Protein Disulfide-Isomerases