title: A new pair of primers designed for amplification of the ITS region in Tuber species.
abstract: The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of primers able to amplify the internal transcribed spacer region of ribosomal DNA in mycorrhizal fungi, such as truffles. The higher yield of the amplification product demonstrates a better annealing of the new primers to the rDNA, as compared to the universal primers internal transcribed spacer 1 and internal transcribed spacer 4. Therefore, the new primers can be used as an easier and more sensitive tool for the identification of truffle species in any stage of their life cycle, including the mycorrhizal phase.
authors.affiliation: Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Bertini
authors.forename: A
authors.initials: A
authors.lastname: Amicucci
authors.forename: D
authors.initials: D
authors.lastname: Agostini
authors.forename: E
authors.initials: E
authors.lastname: Polidori
authors.forename: L
authors.initials: L
authors.lastname: Potenza
authors.forename: C
authors.initials: C
authors.lastname: Guidi
authors.forename: V
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers / Q000235:genetics*; D004271:DNA, Fungal / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S