Evidence of LMP1-TRAF3 interactions in glycosphingolipid-rich complexes of lymphoblastoid and nasopharyngeal carcinoma cells.
Latent membrane protein 1 (LMP1) is an Epstein-Barr virus (EBV) protein expressed in EBV-transformed B lymphocytes and in approximately 50% of nasopharyngeal carcinomas (NPCs). LMP1 signaling involves several cellular signaling intermediates, especially TNF receptor-associated factors (TRAFs). We have shown previously that LMP1 is highly concentrated in a cell fraction called glycosphingolipid-rich membrane complexes (GSL complexes). We report here that parallel accumulation of LMP1 and TRAF3, but not TRAF1 or TRADD, was observed in GSL complexes from lymphoblastoid and LMP1-positive NPC cells. In contrast, TRAF3 was not concentrated in GSL complexes from LMP1-negative cells. Binding of LMP1 and TRAF3 in GSL complexes was demonstrated in lymphoblastoid and NPC cells, by co-immunoprecipitation with both anti-LMP1 and anti-TRAF3 antibodies.
Ardila-Osorio
H
H
Laboratoire de Biologie des Tumeurs Humaines, UMR 1598 CNRS, Institut Gustave Roussy, Villejuif, France.
Clausse
B
B
Mishal
Z
Z
Wiels
J
J
Tursz
T
T
Busson
P
P
D000818:Animals; D001706:Biopsy; D002051:Burkitt Lymphoma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D002499:Centrifugation, Density Gradient; D006028:Glycosphingolipids / Q000302:isolation & purification / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology; D048008:TNF Receptor-Associated Factor 3; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006028:Glycosphingolipids; D011506:Proteins; D018124:Receptors, Tumor Necrosis Factor; D048008:TNF Receptor-Associated Factor 3; D014763:Viral Matrix Proteins