title: Characteristics of induction of virulence factor expression by activated charcoal in Listeria monocytogenes.
abstract: Activated charcoal has been previously shown to induce in vitro expression of virulence factors by Listeria monocytogenes. In trying to elucidate the nature of the charcoal action, we found that the treatment of brain heart infusion medium with activated charcoal followed by charcoal removal does not result in an increase of virulence factor expression. At the same time, the addition of fresh charcoal to the charcoal-treated medium induces expression, suggesting that the effect of activated charcoal cannot be explained only by changes in medium composition. In addition, we observed that activated charcoal induced expression of virulence factors even when L. monocytogenes was physically separated from charcoal particles by either a nitrocellulose membrane or a thin layer of agar. We propose that the interaction of charcoal with some listerial product(s) might be responsible for the effect observed.
authors.affiliation: Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia. sveta@ermolaeva.msk.su
authors.forename: S
authors.initials: S
authors.lastname: Ermolaeva
authors.forename: Y
authors.initials: Y
authors.lastname: Belyi
authors.forename: I
authors.initials: I
authors.lastname: Tartakovskii
mesh_terms: D002606:Charcoal / Q000494:pharmacology; D003470:Culture Media; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D008089:Listeria monocytogenes / Q000378:metabolism* / Q000472:pathogenicity*; D010740:Phospholipases / Q000096:biosynthesis*; D015398:Signal Transduction
chemical_list: D003470:Culture Media; D006460:Hemolysin Proteins; D002606:Charcoal; D010740:Phospholipases