title: Characterisation of serotonin transport mechanisms in rainbow trout peripheral blood lymphocytes: role in PHA-induced lymphoproliferation.
abstract: In this study, we investigated the serotonin transport mechanisms in rainbow trout (Oncorhynchus mykiss) peripheral blood Lymphocytes. We have observed that the transport of serotonin is a membrane transport process that have the properties of a secondary active transport system. The binding isotherm of [3H]-paroxetine, a serotonin transport blocker, demonstrated a high-affinity binding site with a positive type of cooperativity, Hill coefficient being higher than unity. Known specific inhibitors of the mammalian serotonin transporter significantly inhibited the uptake process in fish lymphocytes. In order to demonstrate the physiological relevance of the serotonin transporter in T-cell activation, we conducted experiments on lymphocytes activated or not by phytohemagglutinin (PHA), a T-cell mitogen. We have observed that addition of PHA for 24hrs, increased the Vmax but not the Km of this transporter. Serotonin uptake inhibitors diminished the PHA-activated proliferation of fish lymphocytes. The intracellular concentrations of cAMP were found to regulate the serotonin uptake and the PHA-stimulated proliferation as the agents known to augment cAMP stimulated serotonin uptake, and inhibited the lymphoproliferation. Inhibitory effects of increased cAMP on the proliferation were reversed by the addition of the nanomolar concentrations of 8-OH-DPAT, a 5-HT1A receptor agonist which is known to diminish the intracellular cAMP concentrations, suggesting that serotonin also regulates PHA-induced proliferation via 5-HT1A membrane receptors in an autocrine manner. These results all together demonstrate that fish lymphocytes possess an active serotonin transporter that is implicated in the proliferation of these immunocompetent cells.
authors.affiliation: Université de Rennes-1, UPRES-A 6026 - Equipe E.M.R., Rennes, France. francois.ferriere@univ-rennes1.fr
authors.forename: F
authors.initials: F
authors.lastname: Ferriere
authors.forename: N A
authors.initials: NA
authors.lastname: Khan
authors.forename: J P
authors.initials: JP
authors.lastname: Meyniel
authors.forename: P
authors.initials: P
authors.lastname: Deschaux
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000502:physiology; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D017686:Oncorhynchus mykiss / Q000097:blood*; D010835:Phytohemagglutinins / Q000037:antagonists & inhibitors; D012701:Serotonin / Q000096:biosynthesis / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D000242:Cyclic AMP; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010835:Phytohemagglutinins; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors