title: Alteration in diastolic function following cardiac cryopreservation at subzero temperatures.
abstract: BACKGROUND
  We have studied the alterations produced in the diastolic function of the left ventricle after applying a protocol of cryopreservation at subzero temperatures.
  
  
  METHODS
  Isolated rabbit hearts and 5% polyethylene glycol (PM 4000) as the cryoprotective agent were used for the study.
  
  
  CONCLUSIONS
  Following cryopreservation we found a statistically significant increase in systolic function. However, the diastolic function shows worsening, with a statistically significant increase (p < 0.05) in mean stiffness, decrease in differential stiffness, (p < 0.05) and upward and leftward displacement of the diastolic pressure-volume curve.
authors.affiliation: Virgen del Rocio Hospital, Seville, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gutiérrez-Carretero
authors.forename: R
authors.initials: R
authors.lastname: Bello-Puentes
authors.forename: J M
authors.initials: JM
authors.lastname: Borrego-Domínguez
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Fernández
authors.forename: J
authors.initials: J
authors.lastname: Muñoz-Garcia
authors.forename: A
authors.initials: A
authors.lastname: Ordóñez-Fernández
mesh_terms: D000818:Animals; D002306:Cardiac Volume / Q000502:physiology; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000627:therapeutic use; D003971:Diastole / Q000502:physiology*; D004548:Elasticity; D005615:Freezing; D005947:Glucose / Q000627:therapeutic use; D016027:Heart Transplantation / Q000502:physiology*; D019188:Organ Preservation Solutions / Q000627:therapeutic use; D011092:Polyethylene Glycols / Q000627:therapeutic use; D011817:Rabbits; D013501:Surface-Active Agents / Q000627:therapeutic use; D013599:Systole / Q000502:physiology; D014325:Tromethamine / Q000627:therapeutic use; D016277:Ventricular Function, Left / Q000502:physiology; D017725:Ventricular Pressure / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents; C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D013501:Surface-Active Agents; D014325:Tromethamine; D011092:Polyethylene Glycols; D005947:Glucose