Renal effects of cP450 arachidonate metabolites in the Lyon hypertensive rat.
This study is aimed at evaluating the influence of the cytochrome P450 arachidonate metabolites on the renal alterations exhibited by the Lyon hypertensive (LH) rat. To that purpose, kidneys were isolated from LH rats and their normotensive (LN) controls and single-pass perfused at different pressure levels before (control conditions) and after cytochrome P450 inhibition by 7-ethoxyresorufin (7-ER, 1 microM). In control conditions, LH kidneys differed from LN ones by an increased preglomerular resistance and a blunted pressure natriuresis. 7-ER, which did not affect the function of LN kidneys, decreased the vascular resistance of LH kidneys, increased their glomerular filtration rate but had no effect on their pressure natriuresis. These results indicate that the renal cytochrome P450 arachidonate metabolism differs between LN and LH rats and is presumably involved in the functional alterations exhibited by LH kidneys.
Monneret
G
G
Department of Physiology and Clinical Pharmacology, CNRS-ESA 5014, Faculty of Pharmacy, Lyon, France.
Sassard
J
J
Benzoni
D
D
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005919:Glomerular Filtration Rate; D006973:Hypertension / Q000201:enzymology* / Q000235:genetics*; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007677:Kidney Function Tests; D008297:Male; D011460:Prostaglandins F / Q000378:metabolism; D051381:Rats; D012079:Renal Circulation / Q000502:physiology; D012964:Sodium / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
D011460:Prostaglandins F; D016718:Arachidonic Acid; D013929:Thromboxane B2; D003577:Cytochrome P-450 Enzyme System; D012964:Sodium; D006152:Cyclic GMP; C100006:prostaglandin F1