Lack of causal relationship between clusterin expression and photoreceptor apoptosis in light-induced retinal degeneration.
Induction of apoptosis in the retina leads to cellular death by molecular mechanisms that are not well understood. Clusterin expression is increased in tissues undergoing apoptosis, including retinal neurodegenerative states, but the causal relationships remain to be clarified. To gain insight into clusterin's role in photoreceptor apoptosis, the cellular distribution of clusterin mRNA was compared with the pattern of apoptotic nuclear labelling in a rat model of light-induced retinal degeneration. In control retinal sections, clusterin mRNA was localized to the retinal pigment epithelium cells, photoreceptor inner segments, inner nuclear layer, and ganglion cell layer. Clusterin expression decreased in photoreceptors and retinal pigment epithelium cells, which progressively degenerated, and increased in preserved inner nuclear layer, in proportion to the duration of light exposure in both cyclic light- and dark-reared animals. These results suggest that clusterin is not causally involved in apoptotic mechanisms of photoreceptor death, but may relate to cytoprotective functions.
Jomary
C
C
Department of Pharmacology, The Rayne Institute, GKT, St. Thomas' Hospital, London, England.
Darrow
R M
RM
Wong
P
P
Organisciak
D T
DT
Neal
M J
MJ
Jones
S E
SE
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D051152:Clusterin; D006023:Glycoproteins / Q000378:metabolism*; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D008027:Light; D008297:Male; D018832:Molecular Chaperones; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism / Q000473:pathology; D012162:Retinal Degeneration / Q000473:pathology / Q000503:physiopathology*
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones