Modulation of hepatic and intestinal glutathione S-transferases and other antioxidant enzymes by dietary lipids in streptozotocin diabetic rats.
Antioxidant enzymes in liver and small intestine were investigated using control and streptozotocin diabetic rats fed diets with 5% olive, sunflower or fish oil for five weeks. In liver, Glutathione Peroxidase and Superoxide Dismutase decreased and in intestine Glutathione-S-transferase (GST) increased by diabetes. In isolated jejunum and ileum, this increase in GST activity was due to an increase in GST-alpha and -mu isoenzymes in jejunum and GST-alpha, mu and -pi in ileum. Since GST plays an important role in protecting tissues from oxidative damage, our results highlight the role of the intestine against free radicals in physiological or pathological situations.
Girón
M D
MD
Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Granada, Spain. mgiron@goliat.ugr.es
Salto
R
R
González
Y
Y
Girón
J A
JA
Nieto
N
N
Periago
J L
JL
Suárez
M D
MD
Hortelano
P
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000074242:Sunflower Oil; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005395:Fish Oils; D007527:Isoenzymes; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase