Comprehensive evaluation of isoprenoid biosynthesis regulation in Saccharomyces cerevisiae utilizing the Genome Reporter Matrix.
Gene expression profiling is rapidly becoming a mainstay of functional genomic studies. However, there have been relatively few studies of how the data from expression profiles integrate with more classic approaches to examine gene expression. This study used gene expression profiling of a portion of the genome of Saccharomyces cerevisiae to explore the impact of blocks in the isoprenoid biosynthetic pathway on the expression of genes and the regulation of this pathway. Approximately 50% of the genes whose expression was altered by blocks in isoprenoid biosynthesis were genes previously known to participate in the pathway. In contrast to this simple correspondence, the regulatory patterns revealed by different blocks, and in particular by antifungal azoles, was complex in a manner not anticipated by earlier studies.
Dimster-Denk
D
D
Acacia Biosciences, Inc., 4136 Lakeside Drive, Richmond, CA 94806, USA.
Rine
J
J
Phillips
J
J
Scherer
S
S
Cundiff
P
P
DeBord
K
K
Gilliland
D
D
Hickman
S
S
Jarvis
A
A
Tong
L
L
Ashby
M
M
D001483:Base Sequence; D065607:Cytochrome P-450 Enzyme Inhibitors; D017931:DNA Primers / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004875:Ergosterol / Q000096:biosynthesis / Q000235:genetics; D005246:Feedback; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D017930:Genes, Reporter; D016681:Genome, Fungal; D000072235:Mating Factor; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010455:Peptides / Q000235:genetics; D010675:Pheromones / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis* / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D058886:Sterol 14-Demethylase; D018631:ras Proteins / Q000235:genetics
D065607:Cytochrome P-450 Enzyme Inhibitors; D017931:DNA Primers; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D010455:Peptides; D010675:Pheromones; D011106:Polyisoprenyl Phosphates; D000072235:Mating Factor; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase; D018631:ras Proteins; D004875:Ergosterol