Cutting edge: requirement of class I signal sequence-derived peptides for HLA-E recognition by a mouse cytotoxic T cell clone.
The human nonclassical MHC class I molecule HLA-E has recently been shown to act as a major ligand for NK cell inhibitory receptors. Using HLA-E-expressing transgenic mice, we produced a cytotoxic T cell clone that specifically recognizes the HLA-E molecule. We report here that this T cell clone lyses HLA-E-transfected RMA-S target cells sensitized with synthetic class I signal sequence nonamers. Moreover, this T cell clone lyses human EBV-infected B lymphocytes, PHA blasts, and PBL, formally demonstrating the surface expression of HLA-E/class I signal-derived peptide complex on human cells. Furthermore, these data show that HLA-E complexed with class I signal sequence-derived peptides is not only a ligand for NK cell inhibitory receptors, but can also trigger cytotoxic T cells (CTL).
Martinozzi
S
S
Mouse Immunogenetics, Institut National de la Santé et de la Recherche Médicale, Unite 462, Saint-Louis, Paris, France.
Pacasova
R
R
Boulouis
H J
HJ
Ulbrecht
M
M
Weiss
E H
EH
Sigaux
F
F
Pla
M
M
D000818:Animals; D002999:Clone Cells / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009842:Oligopeptides / Q000276:immunology; D021382:Protein Sorting Signals / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D000097625:HLA-E Antigens
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides; D021382:Protein Sorting Signals; D000097625:HLA-E Antigens