Biotransformation of glycyrrhizin to 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide by Streptococcus LJ-22, a human intestinal bacterium.
By human intestinal bacteria, glycyrrhizin (18beta-glycyrrhetinic acid-3-O-[beta-D-glucuronopyranosyl-(1-->2)-betaD-glucuro nopyranoside], GL) was metabolized to 18beta-glycyrrhetinic acid (GA): the main pathway metabolized GL to GA by glucuronidases of Bacteroides J-37 and Eubacterium sp. GLH and the minor pathway metabolized GL to 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide (GAMG) by beta-glucuronidase of Streptococcus LJ-22. Beta-Glucuronidase from Streptococcus LJ-22 hydrolyzed GL to GAMG (not GA). The molecular weight and optimal pH of the enzyme were 240 kDa and 5-6.
Kim
D H
DH
College of Pharmacy, Kyung-Hee University, Seoul, Korea.
Lee
S W
SW
Han
M J
MJ
D000818:Animals; D001711:Biotransformation; D002417:Cattle; D005966:Glucuronidase / Q000378:metabolism*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D019695:Glycyrrhizic Acid / Q000493:pharmacokinetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D008970:Molecular Weight; D051381:Rats; D013291:Streptococcus / Q000378:metabolism*; D013379:Substrate Specificity; D013549:Sweetening Agents / Q000378:metabolism*
D013549:Sweetening Agents; C052400:glycyrrhetyl 3-monoglucuronide; D019695:Glycyrrhizic Acid; D005966:Glucuronidase; D006034:Glycyrrhetinic Acid