Salivary glands of the sand fly Phlebotomus papatasi contain pharmacologically active amounts of adenosine and 5'-AMP.
Salivary gland homogenates of the sand fly Phlebotomus papatasi contain large amounts of adenosine and 5'-AMP, of the order of 1 nmol per pair of glands, as demonstrated by liquid chromatography, ultraviolet spectrometry, mass spectrometry and bioassays. These purines, 75-80 % of which are secreted from the glands following a blood meal, have vasodilatory and anti-platelet activities and probably help the fly to obtain a blood meal. Salivary 5'-AMP is also responsible for the previously reported protein phosphatase inhibitor in the salivary glands of P. papatasi, which is shown to be artifactual in nature as a result of allosteric modification by AMP of the phosphatase substrate used (phosphorylase a).
Ribeiro
J M
JM
Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Building 4, Room 126, Bethesda, MD 20892-0425, USA. Jribeiro@Atlas.Niaid.NIH.Gov
Katz
O
O
Pannell
L K
LK
Waitumbi
J
J
Warburg
A
A
D000241:Adenosine / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D001769:Blood; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors; D005260:Female; D005502:Food; D008264:Macrophages / Q000201:enzymology; D013058:Mass Spectrometry; D051379:Mice; D008809:Mice, Inbred C3H; D010691:Phlebotomus / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010975:Platelet Aggregation Inhibitors; D011817:Rabbits; D012469:Salivary Glands / Q000737:chemistry* / Q000378:metabolism; D014665:Vasodilator Agents
D004791:Enzyme Inhibitors; D010975:Platelet Aggregation Inhibitors; D014665:Vasodilator Agents; D000249:Adenosine Monophosphate; D010749:Phosphoprotein Phosphatases; D000241:Adenosine