title: Circulating myeloperoxidase and anti-myeloperoxidase antibody in patients with vasculitis.
abstract: To evaluate a role of myeloperoxidase (MPO) and antibody to myeloperoxidase (anti-MPO) in vasculitis, MPO and anti-MPO were determined by enzyme-linked immunosorbent assays in sera from 43 patients with vasculitis, 40 with rheumatoid arthritis, 36 with systemic lupus erythematosus (SLE), 23 with mixed connective tissue disease, 13 with systemic sclerosis, 22 with polymyositis/dermatomyositis, 18 with Sjögren's syndrome, and 30 normal controls. Kidney and lung sections from patients with vasculitis were stained for MPO. Anti-MPO titers were significantly higher (p<0.005) in the patients with vasculitis (mean+/- SD absorbance at 405 nm: 0.53 +/- 0.37) than in any other groups (0.15 +/- 0.04 to approximately 0.21 +/- 0.11). MPO levels in patients with vasculitis were comparable with those in patients with other diseases except SLE. In two patients with vasculitis, anti-MPO decreased sharply with simultaneous increases in MPO 1-2 weeks after they developed pulmonary hemorrhage. Numerous cells positive for MPO infiltrated the Bowman's spaces. These results indicate that MPO may contribute to the pathogenesis of vasculitis and a sudden fall in anti-MPO may predict a poor prognosis in some cases.
authors.affiliation: Division of Rheumatology and Clinical Immunology, Jichi Medical School, Tochigi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Minota
authors.forename: S
authors.initials: S
authors.lastname: Horie
authors.forename: A
authors.initials: A
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Iwamoto
authors.forename: T
authors.initials: T
authors.lastname: Yoshio
authors.forename: A
authors.initials: A
authors.lastname: Mimori
authors.forename: J
authors.initials: J
authors.lastname: Masuyama
authors.forename: S
authors.initials: S
authors.lastname: Kano
mesh_terms: D000818:Animals; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis; D023082:Defensins; D003882:Dermatomyositis / Q000097:blood / Q000276:immunology; D006801:Humans; D007678:Kidney Glomerulus / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation; D008947:Mixed Connective Tissue Disease / Q000097:blood / Q000276:immunology; D009195:Peroxidase / Q000032:analysis / Q000097:blood* / Q000276:immunology*; D011506:Proteins / Q000032:analysis; D012595:Scleroderma, Systemic / Q000097:blood / Q000276:immunology; D014657:Vasculitis / Q000097:blood* / Q000276:immunology*
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001798:Blood Proteins; D023082:Defensins; D011506:Proteins; D009195:Peroxidase