Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene.
Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation.
Sugawara
H
H
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan.
Inoue
T
T
Li
C
C
Gotowda
M
M
Hibino
T
T
Takabe
T
T
Kai
Y
Y
D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009154:Mutation; D010944:Plants / Q000737:chemistry*; D010970:Plastocyanin / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D055672:Static Electricity
D003300:Copper; D010970:Plastocyanin