title: Preparation of IgG subclass allotypes from polyclonal IgG.
abstract: Two allotypes have been identified for each of the IgG subclasses IgG1, IgG2 and IgG3. These allotypes are referred to as G1m(a) and G1m(f), G2m(n) and G2m(-n), and G3m(g) and G3m(b). Using a pool of normal human serum and a combination of preparative electrophoresis, DEAE ion-exchange and protein A-Sepharose chromatography, it was possible to separate G1m(f) from G1m(a), G2m(-n) from G2m(n) and G3m(g) from G3m(b). Purification of G2m(-n) molecules is of special interest as no genetic marker has been found to identify this allotype.
authors.affiliation: Department of Pediatrics and Clinical Immunology, University Hospital, University of Lund, Sweden.
authors.forename: V A
authors.initials: VA
authors.lastname: Oxelius
mesh_terms: D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000097:blood* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood* / Q000302:isolation & purification; D007129:Immunoglobulin Gm Allotypes / Q000097:blood; D012685:Sepharose / Q000031:analogs & derivatives; D013205:Staphylococcal Protein A
chemical_list: D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes; D012685:Sepharose; C042030:Staphylococcal protein A-sepharose; D013205:Staphylococcal Protein A