title: Changes in the extracellular profiles of neuroactive amino acids in the rat striatum at the asymptomatic stage of hepatic failure.
abstract: Rats were treated with a hepatotoxin thioacetamide (TAA) and examined 21 days later, when they showed moderate fatty metamorphosis of the liver and morphological changes in brain indicative of excitotoxic neuronal damage, but no evident biochemical or neurophysiological symptoms of hepatic encephalopathy (HE). High-performance liquid chromatography (HPLC) analysis of extracellular amino acids in striatal microdialysates of TAA-treated rats revealed a significant increase in the excitatory amino acids glutamate (Glu) and aspartate (Asp) and their amino acid metabolites glutamine (Gln) and alanine (Ala). Microdialysis in the presence of 50 mM K+ triggered in TAA-treated rats an accumulation of Asp and Glu, and diminished the accumulation of Gln. These effects were virtually absent in control rats. None of the treatments affected the accumulation of the nontransmitter amino acid leucine (Leu). The above changes mirror those previously described in symptomatic HE and are likely to contribute to excitotoxic damage. The basal microdialysate content of taurine (Tau), an amino acid with antioxidant and volume regulatory properties, was 60% lower in TAA-treated rats than in control rats despite its increased blood-to-brain transport. The decrease in extracellular Tau may thus reflect Tau redistribution to adjacent central nervous system (CNS) cells manifesting a cell-protective response. Stimulation with 50 mM K+ increased extracellular Tau in control rats by 182% and in TAA-treated rats by 322%. Stimulation with 100 microM N-methyl-D-aspartate (NMDA) increased extracellular Tau in control rats by 27 % and in TAA-treated rats by as much as 250%. The increase of K+- or NMDA-dependent Tau release may reflect improved cell volume regulation and neuroprotection and contribute to attenuation of neurologic symptoms in rats with liver failure.
authors.affiliation: Department of Neurotoxicology, Medical Research Centre, Polish Academy of Sciences, Warsaw.
authors.forename: W
authors.initials: W
authors.lastname: Hilgier
authors.forename: M
authors.initials: M
authors.lastname: Zielińska
authors.forename: H D
authors.initials: HD
authors.lastname: Borkowska
authors.forename: R
authors.initials: R
authors.lastname: Gadamski
authors.forename: M
authors.initials: M
authors.lastname: Walski
authors.forename: S S
authors.initials: SS
authors.lastname: Oja
authors.forename: P
authors.initials: P
authors.lastname: Saransaari
authors.forename: J
authors.initials: J
authors.lastname: Albrecht
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001692:Biological Transport; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D018846:Excitatory Amino Acids / Q000378:metabolism*; D005234:Fatty Liver / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D005911:Gliosis; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006501:Hepatic Encephalopathy / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D017551:Microdialysis; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000378:metabolism; D013853:Thioacetamide / Q000633:toxicity
chemical_list: D000596:Amino Acids; D018846:Excitatory Amino Acids; D013853:Thioacetamide; D005973:Glutamine; D013654:Taurine; D001224:Aspartic Acid; D018698:Glutamic Acid; D000409:Alanine; D011188:Potassium