title: R.SspD5I is a neoschizomer of HphI producing blunt end DNA fragments.
abstract: The strain Staphylococcus species D5 produces a restriction enzyme. It is the neoschizomer of HphI endonuclease, which cleaves DNA at a distance of eight nucleotides from the recognition sequences producing blunt end DNA fragments: 5'-GGTGA8N /-3' and 3'-CCACT8N /-5'.
authors.affiliation: Institute of Theoretical and Applied Biophysics, RAS, Pushchino, Russia.
authors.forename: L
authors.initials: L
authors.lastname: Zheleznaya
authors.forename: S
authors.initials: S
authors.lastname: Shiryaev
authors.forename: E
authors.initials: E
authors.lastname: Zheleznyakova
authors.forename: N
authors.initials: N
authors.lastname: Matvienko
authors.forename: N
authors.initials: N
authors.lastname: Matvienko
mesh_terms: D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D013210:Staphylococcus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024412:GGTGA-specific type II deoxyribonucleases