Iron overload upregulates haem oxygenase 1 in the lung more rapidly than in other tissues.
Haem oxygenase-1 is upregulated by numerous insults, including oxidative stress, and under such circumstances it is considered to be a protective stratagem. We have measured the haem oxygenase-1 expression in heart, lung and liver tissues of control and iron-overloaded rats. Lung tissue from iron-overloaded rats displayed a significant increase in the haem oxygenase-1 protein but no changes in haem oxygenase-1 mRNA. Conversely, heart tissue showed a significant increase in haem oxygenase-1 mRNA but no changes in haem oxygenase-1 protein. We conclude that during oxidative stress caused by iron overload, lung tissue responds with a rapid upregulation of haem oxygenase-1 levels.
Anning
P B
PB
Unit of Critical Care, Royal Brompton and Harefield NHS Trust, Imperial College of Science, Technology and Medicine, London, UK.
Chen
Y
Y
Lamb
N J
NJ
Mumby
S
S
Quinlan
G J
GJ
Evans
T W
TW
Gutteridge
J M
JM
D000818:Animals; D001794:Blood Pressure; D015971:Gene Expression Regulation, Enzymologic; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis* / Q000235:genetics; D051547:Heme Oxygenase-1; D019190:Iron Overload / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D012333:RNA, Messenger; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1