Microcolumn size-exclusion chromatography coupled with electrospray ionization mass spectrometry.
Microcolumn (250 x 0.5 mm I.D.) size-exclusion chromatography was implemented for the separation of polydisperse mixtures prior to electrospray ionization (ESI) mass spectrometric detection. An improved separation, compared to conventional-bore SEC, was demonstrated upon coupling with ESI quadrupole ion-trap mass spectrometry and a Fourier transform ion cyclotron resonance instrument for the separation of individual oligomers present in octylphenoxypoly(ethoxy)ethanol.
Prokai
L
L
University of Florida, College of Pharmacy, Center for Drug Discovery, Gainesville 32610-0497, USA.
Aaserud
D J
DJ
Simonsick
W J
WJ
D002850:Chromatography, Gel / Q000295:instrumentation*; D005583:Fourier Analysis; D013058:Mass Spectrometry / Q000295:instrumentation*; D017830:Octoxynol / Q000737:chemistry
D017830:Octoxynol