title: Laser pyrolysis products-genotoxic, clastogenic and mutagenic effects of the particulate aerosol fractions.
abstract: Laser therapy has gained wide acceptance and application in many medical disciplines. Nevertheless, during surgical procedures, the thermal destruction of tissue creates a smoke plume. Recent research data indicate that pyrolysates liberated during vaporisation of tissue induce DNA damage. However, assessing potential health hazards during medical laser treatment requires comprehensive insight into the cytotoxic, genotoxic, clastogenic and mutagenic capacity of laser pyrolysis products (LPP). Therefore, the aim of this study was to evaluate the cytotoxic, genotoxic, clastogenic and mutagenic potential of substances resulting from laser irradiation. Four different types of porcine tissues were irradiated with a surgical CO2 laser, the aerosols were sampled under defined conditions and subjected to the SCE test, micronucleus test and the HPRT test. The results showed that the pyrolysis products are strong inducers of cytotoxic effects. The pyrolysis products induced positive effects in the SCE test, micronucleus test and the HPRT test. The ability and extent to induce genotoxic and mutagenic effects turned out to be dependent on the type of tissue that had been irradiated. In general, the effects were most pronounced with liver pyrolysate. In all test systems, a clear dose relationship could be established. In conclusion, we were able to prove that the particulate fraction of laser pyrolysis aerosols originating from biological tissues undoubtedly have to be classified as cytotoxic, genotoxic, clastogenic and mutagenic. Therefore, they could be potential health hazards for humans.
authors.affiliation: Department of Occupational, Social and Environmental Medicine, University of Ulm, Albert-Einstein Allee 11, D 89070, Ulm, Germany. ulla.plappert@medizin.uni-ulm.de
authors.forename: U G
authors.initials: UG
authors.lastname: Plappert
authors.forename: B
authors.initials: B
authors.lastname: Stocker
authors.forename: R
authors.initials: R
authors.lastname: Helbig
authors.forename: T M
authors.initials: TM
authors.lastname: Fliedner
authors.forename: H J
authors.initials: HJ
authors.lastname: Seidel
mesh_terms: D000273:Adipose Tissue / Q000528:radiation effects*; D000328:Adult; D000336:Aerosols / Q000633:toxicity; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000528:radiation effects; D007834:Lasers / Q000009:adverse effects*; D008099:Liver / Q000528:radiation effects*; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D015162:Micronucleus Tests; D018482:Muscle, Skeletal / Q000528:radiation effects*; D009152:Mutagenicity Tests; D012854:Sister Chromatid Exchange; D013552:Swine
chemical_list: D000336:Aerosols; D007041:Hypoxanthine Phosphoribosyltransferase