The MobA-linked primase is the only replication protein of R1162 required for conjugal mobilization.
Cells newly transformed with plasmid R1162 DNA were used as donors in conjugal matings to determine if the plasmid replication genes are necessary for transfer. An intact system for vegetative replication is not required for transfer at normal frequency, but the plasmid primase, in the form linked to the nickase, must be present in donor cells.
Henderson
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Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
Meyer
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D001426:Bacterial Proteins; D003227:Conjugation, Genetic; D019915:DNA Primase / Q000235:genetics*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008040:Genetic Linkage; D010957:Plasmids / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D019915:DNA Primase