Use of orchiectomy and testosterone replacement to explore meal number-to-meal size relationship in male rats.
Because food intake is a function of meal number and meal size and because gender-related hormones are involved in feeding regulation, we explored effects of orchiectomy and testosterone replacement on the relationship between meal number and size and changes in resulting feeding patterns in adult male rats, randomized into orchiectomy and sham-operation groups. A rat eater meter measured feeding indexes for 1 wk before and 2 wk after castration and during 8 days of testosterone replacement. Orchiectomy leads to an immediate change in the meal number-to-size relationship, resulting in 1) change in pattern of feeding; 2) a significant decrease in dark-phase meal number; 3) a significant increase in dark-phase meal size, but insufficient to offset decrease in meal number, so total food intake significantly decreased during dark phase; 4) no significant change in light-phase meal number; and 5) an increase in meal size leading to an increased food intake during light phase, which offset decreased food intake in dark cycle and resulted in no net significant change in food intake after orchiectomy. Testosterone replacement acutely reversed effects of orchiectomy on meal number-to-meal size relationship, restoring feeding pattern. Data suggest that androgens immediately influence the meal number-to-meal size relationship. The speed of onset seen after orchiectomy suggests that the influence of testosterone on food intake may also occur partially via a nongenomic effect.
Chai
J K
JK
Neuroscience Program, Surgical Metabolism and Nutrition Laboratory, Department of Surgery, University Hospital, State University of New York Health Science Center, Syracuse, New York 13210, USA.
Blaha
V
V
Meguid
M M
MM
Laviano
A
A
Yang
Z J
ZJ
Varma
M
M
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D002940:Circadian Rhythm / Q000502:physiology; D004435:Eating / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D008297:Male; D009919:Orchiectomy; D051381:Rats; D011916:Rats, Inbred F344; D013739:Testosterone / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
D018931:Antineoplastic Agents, Hormonal; D013739:Testosterone