Antibody purification methods.
Antibodies (Abs) from the sera of patients with autoimmune diseases are reported to have different catalytic functions. Their recovery by efficient purification methods is, therefore, a crucial step. This article reviews different available methods for their recovery and emphasizes a new approach, namely adsorbents with immobilized histidine, which allows a good purification both in yield and purity of Abs, with the additional advantage of using gentle elution conditions. This, in turn, will ensure the recovery of intact (nondenatured) catalytically functional Abs, directly from the sera.
Vijayalakshmi
M A
MA
Laboratoire d'Interactions Moléculaires et Technologie des Separations, CNRS-UPRES A 6022; Université de Technologie de Compiegne, France. viji@utc.fr
D000906:Antibodies / Q000302:isolation & purification*; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D002846:Chromatography, Affinity; D006639:Histidine; D006801:Humans
D000906:Antibodies; D006639:Histidine