title: Comparison of three commercial systems for the identification of germ-tube negative yeast species isolated from clinical specimens.
abstract: Three commercial systems were evaluated for their ability to identify 171 germ-tube negative yeasts isolated from clinical specimens. The Yeast Biochemical Card and Analytical Profile Index 20 AUX identified 97% of 171 strains tested. The Biolog system had poor clinical utility: only 48% of strains were identified. For Yeast Biochemical Card and Analytical Profile Index 20 AUX, 9% and 6%, respectively, required repeat testing and both systems required supplemental tests for 28% of the strains. These observations indicate that considerable expertise and a battery of reagents in addition to the basic systems are required for accurate identification of germ-tube negative yeasts.
authors.affiliation: National Centre for Mycology, University of Alberta Hospital, Edmonton, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Sand
authors.forename: R P
authors.initials: RP
authors.lastname: Rennie
mesh_terms: D008828:Microbiological Techniques; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification