The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB/c mice.
2,3-Dihydroxybenzoic acid (DHBA) is the only siderophore described for Brucella, and previous studies suggested that DHBA might contribute to the capacity of these organisms to persist in host macrophages. Employing an isogenic siderophore mutant (DeltaentC) constructed from virulent Brucella abortus 2308, however, we found that production of DHBA is not required for replication in cultured murine macrophages or for the establishment and maintenance of chronic infection in the BALB/c mouse model.
Bellaire
B H
BH
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932, USA.
Elzer
P H
PH
Baldwin
C L
CL
Roop
R M
RM
D000818:Animals; D002003:Brucella abortus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D002006:Brucellosis / Q000209:etiology / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D005798:Genes, Bacterial; D062385:Hydroxybenzoates / Q000378:metabolism*; D019751:Intramolecular Transferases / Q000235:genetics; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D017262:Siderophores / Q000378:metabolism*; D014774:Virulence / Q000235:genetics / Q000502:physiology
D062385:Hydroxybenzoates; D017262:Siderophores; C009135:2,3-dihydroxybenzoic acid; D019751:Intramolecular Transferases; C058486:isochorismate synthase