Discrimination between ribonuclease H- and ribonuclease L-mediated RNA degradation by 2'-O-methylated 2-5A-antisense oligonucleotides.
2',5'-Oligoadenylate (2-5A) antisense chimeric oligonucleotides were synthesized containing varying 2'-O-methyl-ribonucleotide substitution patterns in the antisense domain. The ability of these composite oligonucleotides to mediate RNase H- and RNase L-catalyzed RNA degradation showed that these two enzymes have different activation requirements.
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Section on Biomedical Chemistry, Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805, USA.
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D000227:Adenine Nucleotides / Q000235:genetics*; D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D008745:Methylation; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000235:genetics*; D012313:RNA / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism*
D000227:Adenine Nucleotides; D016376:Oligonucleotides, Antisense; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D012313:RNA; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D016914:Ribonuclease H