Frequencies of SDF-1 chemokine, CCR-5, and CCR-2 chemokine receptor gene alleles conferring resistance to human immunodeficiency virus type 1 and AIDS in Kuwaitis.
The frequencies of three mutations conferring resistance to HIV/AIDS were determined in a population sample of native Kuwaitis. The CCR2-641, SDF1-3'A, and CCR5-m303 mutations were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) tests using restriction endonucleases Bsa BI, Msp I, and Hinc II, respectively. The frequency of the mutant alleles were: for CCR2-641, 0.1195 (95% CI 0.0801-0.1694); for SDF1-3'A, 0.2593 (95% CI 0.2024-0.3231), and for CCR5-m303, less than 0.0025. Thus, the CCR2-641 and especially SDF1-3'A mutations are sufficiently common in Arabs and can be used for prognostic genotyping in HIV-infected individuals from the Gulf countries.
Voevodin
A
A
Department of Microbiology, Faculty of Medicine, Kuwait University, Safat. voevodin@hsc.kuniv.edu.kw
Samilchuk
E
E
Dashti
S
S
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology; D000483:Alleles; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007730:Kuwait; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000235:genetics*; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000235:genetics*
C515334:CCR2 protein, human; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine