Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK.
Thymus-expressed chemokine (TECK) has been reported to chemoattract dendritic cells, thymocytes, and activated macrophages. Here, we show that TECK is a specific agonist for a human orphan receptor called GPR-9-6. We have determined the cDNA sequence of human GPR-9-6 and cloned the corresponding murine cDNA. Human and murine GPR-9-6 expression is very high in the thymus and low in lymph nodes and spleen. RT-PCR analysis of murine GPR-9-6 expression on murine FACS-sorted thymocyte subpopulations showed that this gene is expressed in both immature and mature T cells. Additions of human or murine TECK to HEK 293/human GPR-9-6 and HEK 293/murine GPR-9-6 transfectants provoked intracytoplasmic calcium mobilization. Human TECK also induced the in vitro migration of HEK 293/human GPR-9-6 cells. These results confirm that GPR-9-6 is a specific receptor for TECK. According to the established nomenclature system, we propose to rename GPR-9-6 as CC chemokine receptor 9 (CCR9).
Zaballos
A
A
Departamento de Inmunología y Oncología, Centro Nacional de Biotecnología, Universidad Autónoma de Madrid, Madrid, Spain.
Gutiérrez
J
J
Varona
R
R
Ardavín
C
C
Márquez
G
G
D000595:Amino Acid Sequence; D000818:Animals; D020013:Calcium Signaling; D019742:Chemokines, CC / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000302:isolation & purification; D054388:Receptors, CCR; D019707:Receptors, Chemokine / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013950:Thymus Gland / Q000166:cytology; D014018:Tissue Distribution
C119309:CC chemokine receptor 9; C108074:CCL25 protein, human; C491541:Ccl25 protein, mouse; D019742:Chemokines, CC; D008024:Ligands; D012333:RNA, Messenger; D054388:Receptors, CCR; D019707:Receptors, Chemokine