title: Development and application of a selective detection method for genetically modified soy and soy-derived products.
abstract: A method has been developed to distinguish between traditional soy beans and transgenic Roundup Ready soy beans, i.e. the glyphosate ('Roundup') resistant soy bean variety developed by Monsanto Company. Glyphosate resistance results from the incorporation of an Agrobacterium-derived 5-enol-pyruvyl-shikimate-3-phosphatesynthase (EPSPS) gene. The detection method developed is based on a nested Polymerase Chain Reaction (PCR) procedure. Ten femtograms of soy bean DNA can be detected, while, starting from whole soy beans, Roundup Ready DNA can be detected at a level of 1 Roundup Ready soy bean in 5000 non-GM soy beans (0.02% Roundup Ready soy bean). The method has been applied to samples of soy bean, soy-meal pellets and soy bean flour, as well as a number of processed complex products such as infant formula based on soy, tofu, tempeh, soy-based desserts, bakery products and complex meat and meat-replacing products. The results obtained are discussed with respect to practical application of the detection method developed.
authors.affiliation: Department of Food Safety and Health, State Institute for Quality Control of Agricultural Products (RIKILT-DLO), Wageningen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Hoef
authors.forename: E J
authors.initials: EJ
authors.lastname: Kok
authors.forename: E
authors.initials: E
authors.lastname: Bouw
authors.forename: H A
authors.initials: HA
authors.lastname: Kuiper
authors.forename: J
authors.initials: J
authors.lastname: Keijer
mesh_terms: D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D005524:Food Technology; D005818:Genetic Engineering; D006801:Humans; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods*; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant