Clusterin (SGP-2) gene expression is cell cycle dependent in normal human dermal fibroblasts.
In confluent human dermal fibroblasts brought to quiescence (G0) by serum starvation, the S phase peaked at 24 h after serum re-addiction and G2/M phase peaked at 36 h. This was confirmed by titration of h-gas1 mRNA (a marker of G0 phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G0, in parallel with h-gas1 mRNA. At 6 h (roughly G1 phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G0 peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.
Bettuzzi
S
S
Dipartimento di Scienze Biomediche, University of Modena and Reggio Emilia, Modena, Italy. corti@unimo.it
Astancolle
S
S
Guidetti
G
G
Moretti
M
M
Tiozzo
R
R
Corti
A
A
D015152:Blotting, Northern; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D051152:Clusterin; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D006023:Glycoproteins / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins; D012867:Skin / Q000166:cytology; D013997:Time Factors
C495242:CLU protein, human; D018797:Cell Cycle Proteins; D051152:Clusterin; C066709:GAS1 protein, S cerevisiae; C096192:GAS1 protein, human; D058851:GPI-Linked Proteins; D006023:Glycoproteins; D006657:Histones; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins