Altered temperature induction sensitivity of the lambda pR/cI857 system for controlled gene E expression in Escherichia coli.
Cell lysis of Gram-negative bacteria can be efficiently achieved by expression of the cloned lysis gene E of bacteriophage PhiX174. Gene E expression is tightly controlled by the rightward lambda pR promoter and the temperature-sensitive repressor cI857 on lysis plasmid pAW12. The resulting empty bacterial cell envelopes, called bacterial ghosts, are currently under investigation as candidate vaccines. Expression of gene E is stringently repressed at temperatures up to 30 degrees C, whereas gene E expression, and thus cell lysis, is induced at temperatures higher than 30 degrees C due to thermal inactivation of the cI857 repressor. As a consequence, the production of ghosts requires that bacteria have to be grown at 28 degrees C before the lysis process is induced. In order to reflect the growth temperature of pathogenic bacteria in vivo, it seemed favorable to extend the heat stability of the lambda pR promoter/cI857 repressor system, allowing pathogens to grow at 37 degrees C before induction of lysis. In this study we describe a mutation in the lambda pR promoter, which allows stringent repression of gene E expression at temperatures up to 36 degrees C, but still permits induction of cell lysis at 42 degrees C.
Jechlinger
W
W
Institute of Microbiology and Genetics, University of Vienna, Austria. W.Jechlinger@evax.de
Szostak
M P
MP
Witte
A
A
Lubitz
W
W
D001433:Bacteriolysis; D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology; D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D007763:Lac Operon; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics*
C029555:E protein, bacteriophage X174; D014764:Viral Proteins