title: Alpha2-macroglobulin and eosinophil cationic protein in the allergic airway mucosa in seasonal allergic rhinitis.
abstract: As previously demonstrated in seasonal allergic rhinitis, increased microvascular permeability and eosinophil activation are key features of allergic airway inflammation. In the present study, the hypothesis that exudation of alpha2-macroglobulin may cause the appearance of eosinophil cationic protein (ECP) in the airway lumen was explored. Nasal lavages were carried out using the nasal pool device before and during the pollen season both at baseline and after histamine challenge in 10 children with allergic rhinitis. Nasal lavage fluid levels of alpha2-macroglobulin and ECP were determined. All patients experienced nasal symptoms of allergic rhinitis during the pollen season (p<0.01-0.05). Baseline nasal lavage fluid levels of alpha2-macroglobulin and ECP were increased during the season (p<0.01-0.05) and were found to be well correlated (p<0.0001). Histamine produced concentration-dependent plasma exudation before and during the pollen season, but it was only during the pollen season that this caused an increase in the lavage fluid levels of ECP (p<0.05). These data suggest that exudation of plasma and increased tissue levels and output of eosinophil cationic protein characterize nasal mucosal inflammation in children with seasonal allergic rhinitis. The plasma exudation process in part may account for lumenal entry of eosinophil cationic protein molecules that have been released in mucosal tissue compartments. A combination of induced exudation and nasal lavage may improve the yield of important markers of inflammation in studies of nasal diseases.
authors.affiliation: Dept of Paediatrics, University Hospital, Lund/Malmö, Lund, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Meyer
authors.forename: C G
authors.initials: CG
authors.lastname: Persson
authors.forename: M
authors.initials: M
authors.lastname: Andersson
authors.forename: P
authors.initials: P
authors.lastname: Wollmer
authors.forename: M
authors.initials: M
authors.lastname: Linden
authors.forename: C
authors.initials: C
authors.lastname: Svensson
authors.forename: L
authors.initials: L
authors.lastname: Greiff
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D002648:Child; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D015200:Fluoroimmunoassay; D006632:Histamine; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis*; D008297:Male; D017817:Nasal Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis*; D012260:Ribonucleases; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D014197:Trees; D000511:alpha-Macroglobulins / Q000032:analysis*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D000511:alpha-Macroglobulins; D006632:Histamine; D012260:Ribonucleases