title: Modulation of transducin GTPase activity by chimeric RGS16 and RGS9 regulators of G protein signaling and the effector molecule.
abstract: RGS9, a member of the family of regulators of G protein signaling (RGS), serves as a GTPase-activating protein (GAP) for the transducin alpha-subunit (Gtalpha) in the vertebrate visual transduction cascade. The GAP activity of RGS9 is uniquely potentiated by the gamma-subunit of the effector enzyme, cGMP-phosphodiesterase (Pgamma). In contrast, Pgamma attenuates the GAP effects of several other RGS proteins, including RGS16. We demonstrate here that the Pgamma subunit exerts its effects on the GTPase activity of the Gtalpha-RGS complex via the C-terminal domain, Pgamma-63-87. The structural determinants that control the direction of Pgamma effects on the RGS-Gtalpha system are localized within the RGS domains. The addition of Pgamma caused an increase in the maximal stimulation of Gtalpha GTPase activity by RGS9d without affecting the EC50 value. Modulation of Gtalpha GTPase activity by chimeric RGS16 and RGS9 proteins and Pgamma has been investigated. This analysis suggests that in addition to the differences in primary structures, the overall conformations of the RGS fold in RGS9 and RGS16 are likely to be responsible for the opposite effects of Pgamma on the RGS9 and RGS16 GAP activity. The RGS9 alpha3-alpha5 region constituted the minimal insertion of the RGS9 domain into RGS16 that reversed the inhibitory effect of Pgamma. A model of the RGS9 complex with Gtalpha shows the alpha3-alpha5 helices in RGS9 facing the proximate Pgamma binding site on Gtalpha. Our results and this model demonstrate that the mechanism of potentiation of RGS9 GAP activity by Pgamma involves a more rigid stabilization of the Gtalpha switch regions when Gtalpha is bound to both RGS9 and Pgamma.
authors.affiliation: Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: McEntaffer
authors.forename: M
authors.initials: M
authors.lastname: Natochin
authors.forename: N O
authors.initials: NO
authors.lastname: Artemyev
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000502:physiology; D000818:Animals; D002417:Cattle; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D004357:Drug Synergism; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D008958:Models, Molecular; D010446:Peptide Fragments / Q000502:physiology; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020710:RGS Proteins; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000502:physiology*; D012374:Rod Cell Outer Segment / Q000201:enzymology / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics; D015293:Transducin / Q000378:metabolism*
chemical_list: D020690:GTPase-Activating Proteins; D010446:Peptide Fragments; D011506:Proteins; D020710:RGS Proteins; C107413:RGS16 protein; D011993:Recombinant Fusion Proteins; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D020558:GTP Phosphohydrolases; D015293:Transducin