Analysis of cyclin D1 in de novo and relapsed childhood acute lymphoblastic leukemia.
In a retrospective analysis we investigated 59 children with de novo acute lymphoblastic leukemia (ALL) and 28 patients with relapsed ALL for the expression of cyclin D1 using RT-PCR. In addition, the relationships of cyclin D1 to the retinoblastoma tumor suppressor gene and the Ki-67-expression were analyzed. Cyclin D1-mRNA was detectable in 42 out of 58 patients with de novo ALL (72%) and in 26 out of 28 relapsed patients (93%). The blast cells of the relapsed patients contained significantly higher levels of cyclin D1-mRNA compared with the de novo group (p = 0.0007). The cyclin D1 expression was independent of the proliferative activity of the cells and inversely correlated to the expression of the retinoblastoma tumor suppressor gene (r = -0.27, p = 0.03). Prognostic considerations using Kaplan Meier estimates for the relapse-free interval showed that patients with high cyclin D1- mRNA levels had a poorer prognosis (p = 0.046).
Sauerbrey
A
A
University of Jena, Department of Pediatrics, Germany.
Häfer
R
R
Zintl
F
F
Volm
M
M
D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D019938:Cyclin D1 / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007223:Infant; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D012008:Recurrence; D016160:Retinoblastoma Protein / Q000378:metabolism; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001613:beta 2-Microglobulin / Q000378:metabolism
D019394:Ki-67 Antigen; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D001613:beta 2-Microglobulin; D019938:Cyclin D1