title: Prolonged induction of IL-8 gene expression in a human fibroblast cell line infected with reovirus serotype 1 strain Lang.
abstract: Viruses which infect mucosal surfaces commonly infect these particular anatomical sites based on both the virion structure and the interaction of the virus with a particular microenvironment. We infected a human lung epithelial cell line, a human gut epithelial cell line, and a human lung fibroblast cell line with reovirus 1/L to explore how this natural isolate of both the lung and the gut may interact with mucosal surfaces. While reovirus infection of the gut and lung epithelial cell lines was lytic, a chronic infection was established in the human lung fibroblast cell line. All three cell lines also produced interleukin-8 (IL-8) after infection with reovirus 1/L, and IL-8 production was not dependent upon viral replication. A prolonged production of IL-8 was observed in the chronically infected lung fibroblast cell line, suggesting that this mucosal population may be involved in the generation of inflammatory responses after the resolution of the initial lytic infection of the epithelium. These studies provide an in vitro model system for analyzing the interaction of reovirus 1/L with resident mucosal cell populations.
authors.affiliation: Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425, USA.
authors.forename: D
authors.initials: D
authors.lastname: Hamamdzic
authors.forename: S
authors.initials: S
authors.lastname: Altman-Hamamdzic
authors.forename: S C
authors.initials: SC
authors.lastname: Bellum
authors.forename: T J
authors.initials: TJ
authors.lastname: Phillips-Dorsett
authors.forename: S D
authors.initials: SD
authors.lastname: London
authors.forename: L
authors.initials: L
authors.lastname: London
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D018928:Immunity, Mucosal; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics*; D009092:Mucous Membrane / Q000276:immunology / Q000821:virology; D018165:Orthoreovirus / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012088:Reoviridae Infections / Q000235:genetics / Q000276:immunology; D012703:Serotyping
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger