Laparoscopic insufflation with room air causes exaggerated interleukin-6 response.
BACKGROUND
The gut is a central organ in the postoperative stress reaction. We previously reported that measuring gut-mucosal cytokines may more accurately reflect the response to operative stress. Additionally, we have shown that the gut demonstrates a blunted cytokine response after laparoscopy as compared with laparotomy.


METHODS
To further investigate whether this differential response is caused by exposure of the peritoneal cavity to general atmospheric air during laparotomy, 80 A/J mice were randomized equally into four groups: CD (carbon dioxide [CO2] pneumoperitoneum), RA (room air pneumoperitoneum), AP (anesthesia and port insertion only), and PC (pure control, no intervention). Pneumoperitoneum was established and maintained at 3 mmHg for 60 min. All the mice were killed 4 h after the intervention. Jejunal mucosa and serum samples were collected and analyzed for interleukin-6 (IL-6) levels. Results were analyzed by analysis of variance (ANOVA).


RESULTS
Gut-mucosal IL-6 in the RA group was significantly higher than in all other groups: RA, 1,354.5 +/- 117.9* vs. CD, 964.3 +/- 114.0 vs. AP, 960.2 +/- 86.2 vs. PC, 908.0 +/- 83.6; *p < 0.05. The CD group did not show a significant increase in gut-mucosal IL-6 as compared with the AP and PC groups. Similarly, RA resulted in significant increases in serum IL-6 as compared with AP and PC, whereas CD showed no significant difference: RA, 161.3 +/- 66.2* vs. 95.1 +/- 1 vs. AP, 10.6 +/- 5.3 vs. PC, undetectable; *p < 0.05. There was no difference in serum IL-6 level between CD or any of the other groups.


CONCLUSIONS
Exposure of the peritoneal cavity to atmospheric air, independently of the trauma of abdominal access, causes an exaggerated serum and gut mucosal IL-6 response 4 h after intervention. The beneficial effect of CO2 laparoscopy may be caused by the exclusion of general atmospheric air from the peritoneal cavity.
Tung
P H
PH
Department of Surgery, Emory University School of Medicine, 1364 Clifton Road, NE, Suite H 122, Atlanta, GA 30322, USA.
Smith
C D
CD
D000388:Air; D000704:Analysis of Variance; D000818:Animals; D002245:Carbon Dioxide; D004797:Enzyme-Linked Immunosorbent Assay; D007327:Insufflation / Q000379:methods*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000378:metabolism*; D010535:Laparoscopy; D007813:Laparotomy; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D011897:Random Allocation
D015850:Interleukin-6; D002245:Carbon Dioxide