Antisense of c-fos gene attenuates Fos expression in the spinal cord induced by unilateral constriction of the sciatic nerve in the rat.
Sciatic nerve constriction induces expression of c-fos protein in dorsal horn neurons of the spinal cord. Intrathecal administration of c-fos antisense (30 nmol/20 microl) into the lumbar region (L1-L5) 18 h prior to nerve ligation attenuated 80% of Fos-immunoreactivity 90 min after ligation compared to rats infused with c-fos sense or saline. Thus, c-fos antisense may be a useful tool in assessing the role of the c-fos gene in an animal model of neuropathic pain.
Huang
W
W
Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA.
Simpson
R K
RK
D000818:Animals; D007839:Functional Laterality; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*
D020319:Oligodeoxyribonucleotides, Antisense; D016760:Proto-Oncogene Proteins c-fos