title: Whole-body protein turnover and resting energy expenditure in obese, prepubertal children.
abstract: BACKGROUND
  Obesity is becoming more frequent in children; understanding the extent to which this condition affects not only carbohydrate and lipid metabolism but also protein metabolism is of paramount importance.
  
  
  OBJECTIVE
  We evaluated the kinetics of protein metabolism in obese, prepubertal children in the static phase of obesity.
  
  
  METHODS
  In this cross-sectional study, 9 obese children (x +/- SE: 44+/-4 kg, 30.9+/-1.5% body fat) were compared with 8 lean (28+/-2 kg ,16.8+/-1.2% body fat), age-matched (8.5+/-0.2 y) control children. Whole-body nitrogen flux, protein synthesis, and protein breakdown were calculated postprandially over 9 h from 15N abundance in urinary ammonia by using a single oral dose of [15N]glycine; resting energy expenditure (REE) was assessed by indirect calorimetry (canopy) and body composition by multiple skinfold-thickness measurements.
  
  
  RESULTS
  Absolute rates of protein synthesis and breakdown were significantly greater in obese children than in control children (x +/- SE: 208+/-24 compared with 137+/-14 g/d, P < 0.05, and 149+/-20 compared with 89+/-13 g/d, P < 0.05, respectively). When these variables were adjusted for fat-free mass by analysis of covariance, however, the differences between groups disappeared. There was a significant relation between protein synthesis and fat-free mass (r = 0.83, P < 0.001) as well as between protein synthesis and REE (r = 0.79, P < 0.005).
  
  
  CONCLUSIONS
  Obesity in prepubertal children is associated with an absolute increase in whole-body protein turnover that is consistent with an absolute increase in fat-free mass, both of which contribute to explaining the greater absolute REE in obese children than in control children.
authors.affiliation: Institute of Physiology, Faculty of Medicine, University of Lausanne, Switzerland. Yves.schutz@i.physiol.unil.ch
authors.forename: Y
authors.initials: Y
authors.lastname: Schutz
authors.forename: C M
authors.initials: CM
authors.lastname: Rueda-Maza
authors.forename: M
authors.initials: M
authors.lastname: Zaffanello
authors.forename: C
authors.initials: C
authors.lastname: Maffeis
mesh_terms: D002648:Child; D004734:Energy Metabolism; D006801:Humans; D009584:Nitrogen / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D011627:Puberty
chemical_list: D011506:Proteins; D009584:Nitrogen