Soy-derived antiapoptotic fractions protect gastrointestinal epithelium from damage caused by methotrexate treatment in the rat.
The ability of a previously described soy-derived antiapoptotic fraction (SDAAF), a soy water extract (Lexirin), and raw soy flour to inhibit methotrexate (MTX)-induced gastrointestinal damage was evaluated by histological examination of duodenal/jejunal sections from MTX-treated rats. Male Sprague-Dawley rats were fed diets containing casein as a sole protein source or diets supplemented with fractions isolated from soy (SDAAF or Lexirin) before and after MTX treatment. The soy fractions were also shown to inhibit serum deprivation-induced programmed cell death (apoptosis) in mouse embryonic C3H10T1/2 cells. Protein sequence (Lexirin) and enzyme activity (Lexirin and SDAAF) were also analyzed. Rats that received SDAAF- and Lexirin-supplemented diets had significantly reduced necrotic and apoptotic damage in the duodenal mucosa, as demonstrated by difference in villi height, mitotic activity, epithelial cell height, and inflammatory response.
Logvinova
A V
AV
LXR Biotechnology, Inc., Richmond, CA 94804, USA.
Foehr
M W
MW
Pemberton
P A
PA
Khazalpour
K M
KM
Funk-Archuleta
M A
MA
Bathurst
I C
IC
Tomei
L D
LD
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D002364:Caseins / Q000494:pharmacology*; D005770:Gastrointestinal Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008727:Methotrexate / Q000633:toxicity*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000737:chemistry / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology
D000964:Antimetabolites, Antineoplastic; D002364:Caseins; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; D008727:Methotrexate