DNA sequencing and analysis of a 40 kb region from the right arm of chromosome II from Schizosaccharomyces pombe.
We have determined the complete nucleotide sequence of a 39,648 bp segment, contained in cosmid c32F12, derived from the right arm of chromosome II from the fission yeast Schizosaccharomyces pombe. Computer analysis of the sequence revealed the presence of 15 non-overlapping open reading-frames (ORFs) longer than 300 bp and one tRNA-Thr gene. Six ORFs correspond to the previously known rec14+, tug1+, rum1+, pch1+, gpd1+ and cyr1+ genes. Five ORFs code for putative proteins with significant homology to proteins from other organisms. SPBC32F12.01c shows considerable similarity to human neutral sphingomyelinase, whereas SPBC32F12.03c, SPBC32F12.10 and SPBC32F12.14 exhibit strong homology to glutathione peroxidase, phosphoglucomutase and ubiquitin protein ligase E-3 components from various organisms, respectively. The four remaining ORFs identified show weak or non-significant homology to previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession Number AL023796.
Sánchez
M
M
Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca/CSIC, Spain.
del Rey
F
F
Domínguez
A
A
Moreno
S
S
Revuelta
J L
JL
D000595:Amino Acid Sequence; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000235:genetics; D006801:Humans; D008025:Ligases / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010733:Phosphoglucomutase / Q000235:genetics; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
D004271:DNA, Fungal; D005656:Fungal Proteins; D012345:RNA, Transfer, Amino Acid-Specific; D005979:Glutathione Peroxidase; D044767:Ubiquitin-Protein Ligases; D013108:Sphingomyelin Phosphodiesterase; D010733:Phosphoglucomutase; D008025:Ligases