title: An impaired routing of wild-type aquaporin-2 after tetramerization with an aquaporin-2 mutant explains dominant nephrogenic diabetes insipidus.
abstract: Autosomal recessive and dominant nephrogenic diabetes insipidus (NDI), a disease in which the kidney is unable to concentrate urine in response to vasopressin, are caused by mutations in the aquaporin-2 (AQP2) gene. Missense AQP2 proteins in recessive NDI have been shown to be retarded in the endoplasmic reticulum, whereas AQP2-E258K, an AQP2 mutant in dominant NDI, was retained in the Golgi complex. In this study, we identified the molecular mechanisms underlying recessive and dominant NDI. Sucrose gradient centrifugation of rat and human kidney proteins and subsequent immunoblotting revealed that AQP2 forms homotetramers. When expressed in oocytes, wild-type AQP2 and AQP2-E258K also formed homotetramers, whereas AQP2-R187C, a mutant in recessive NDI, was expressed as a monomer. Upon co-injection, AQP2-E258K, but not AQP2-R187C, was able to heterotetramerize with wild-type AQP2. Since an AQP monomer is the functional unit and AQP2-E258K is a functional but misrouted water channel, heterotetramerization of AQP2-E258K with wild-type AQP2 and inhibition of further routing of this complex to the plasma membrane is the cause of dominant NDI. This case of NDI is the first example of a dominant disease in which the 'loss-of-function' phenotype is caused by an impaired routing rather than impaired function of the wild-type protein.
authors.affiliation: Department of Cell Physiology, University of Nijmegen, 6500HB Nijmegen, The Netherlands.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kamsteeg
authors.forename: T A
authors.initials: TA
authors.lastname: Wormhoudt
authors.forename: J P
authors.initials: JP
authors.lastname: Rijss
authors.forename: C H
authors.initials: CH
authors.lastname: van Os
authors.forename: P M
authors.initials: PM
authors.lastname: Deen
mesh_terms: D000818:Animals; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D018500:Diabetes Insipidus, Nephrogenic / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009154:Mutation; D009865:Oocytes; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000378:metabolism; D014867:Water / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: C496023:AQP2 protein, human; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins; D011994:Recombinant Proteins; D014867:Water