Effect of vitamin E in gastric mucosal injury induced by ischaemia-reperfusion in nitric oxide-depleted rats.
BACKGROUND
Neutrophil infiltration and lipid peroxide accumulation are involved in reperfusion-induced gastric mucosal injury in nitric oxide-depleted rats.


OBJECTIVE
To assess the effect of vitamin E on this injury.


METHODS
After ischaemia-reperfusion, the total area of erosions, lipid peroxide contents in gastric mucosa, and gastric neutrophil accumulation were compared between nitric oxide-depleted rats with deficient, normal, and increased vitamin E intake over 8 weeks. Thiobarbituric acid-reactive substances and tissue-associated myeloperoxidase activity were measured in gastric mucosa as indices of lipid peroxidation and neutrophil infiltration.


RESULTS
The total area of erosions was significantly increased in the vitamin E-deficient group compared with the sufficient-intake and vitamin-supplemented groups. Both thiobarbituric acid-reactive substances and myeloperoxidase activity also were significantly increased in the vitamin E-deficient group compared with others. The total area of erosions closely paralleled the increases in both thiobarbituric acid-reactive substances and myeloperoxidase activity.


CONCLUSIONS
These results indicate that the inhibition of lipid peroxidation and interference with neutrophil infiltration by vitamin E may be responsible for its cytoprotective effect in ischaemia-reperfusion.
Naito
Y
Y
First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan. ynaito@koto.kpu-m.ac.jp
Yoshikawa
T
T
Matsuyama
K
K
Yagi
N
N
Kasai
K
K
Sugimoto
N
N
Masui
Y
Y
Yoshida
N
N
Kondo
M
M
D000818:Animals; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D020011:Protective Agents / Q000378:metabolism / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications / Q000503:physiopathology / Q000517:prevention & control*; D013272:Stomach Diseases / Q000209:etiology / Q000517:prevention & control*; D014810:Vitamin E / Q000378:metabolism / Q000627:therapeutic use*; D014811:Vitamin E Deficiency / Q000503:physiopathology
D020011:Protective Agents; D014810:Vitamin E; D009569:Nitric Oxide