Cytokine production in acute versus chronic human Schistosomiasis mansoni: the cross-regulatory role of interferon-gamma and interleukin-10 in the responses of peripheral blood mononuclear cells and splenocytes to parasite antigens.
The contribution of interleukin (IL)-10 and interferon (IFN)-gamma to the regulation of type 1 and type 2 cytokine responses was investigated in Brazilians with different clinical forms of schistosomiasis mansoni. Cells from members of a family with acute intestinal schistosomiasis responded to schistosomal soluble egg antigen (SEA) or soluble adult worm antigen preparation (SWAP) with greater amounts of IFN-gamma than did cells from several patients with chronic intestinal schistosomiasis; IL-10 levels were similar. Neutralization of IL-10 had no effect on the SEA-specific IFN-gamma response in patients with acute infection, whereas SWAP-induced IFN-gamma was increased in both groups. Anti-IL-10 also up-regulated SEA-specific IFN-gamma protein and mRNA responses in most splenocyte cultures from hepatosplenic schistosomiasis patients but had no effect on antigen-specific IL-4 or IL-5 production. Neutralization of IFN-gamma resulted in a comparable increase in SWAP-specific IL-10 and IL-5, while IL-4 was not affected. These studies demonstrate that early disease in schistosomiasis is associated with a significant IFN-gamma response and that IL-10 contributes to the suppression of that response during both early and chronic infection.
Montenegro
S M
SM
Department de Imunologia, Centro de Pesquisas Aggeu Magalhaes-FIOCRUZ, Cidade Universitaria, 7472, 50670-420 Recife-PE, Brazil. silvia@cpqam.fiocruz.br
Miranda
P
P
Mahanty
S
S
Abath
F G
FG
Teixeira
K M
KM
Coutinho
E M
EM
Brinkman
J
J
Gonçalves
I
I
Domingues
L A
LA
Domingues
A L
AL
Sher
A
A
Wynn
T A
TA
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000947:Antigens, Helminth / Q000276:immunology*; D002908:Chronic Disease; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006529:Hepatomegaly; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013163:Splenomegaly
D000947:Antigens, Helminth; D016207:Cytokines; D012333:RNA, Messenger; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma