The cellular form of human fibronectin as an adhesion target for the S fimbriae of meningitis-associated Escherichia coli.
The adhesion of the S fimbriae of meningitis-associated Escherichia coli O18ac:K1:H7 to the cellular and the plasma forms of human fibronectin was studied. E. coli HB101(pAZZ50) expressing the complete S-fimbria II gene cluster of E. coli O18 adhered to cellular fibronectin (cFn) on glass but not to plasma fibronectin (pFn). Adhesion to cFn was specifically inhibited by neuraminidase treatment of cFn as well as by incubation of the bacteria with sialyl-alpha2-3-lactose, a receptor analog of the S fimbriae. No significant adhesion to cFn or pFn was detected with E. coli HB101(pAZZ50-67) expressing S fimbriae lacking the SfaS lectin subunit. Strain HB101(pAZZ50) also adhered to a human fibroblast cell culture known to be rich in cFn, and the adhesion was specifically inhibited in the presence of polyclonal antibodies to cFn. The results show that the SfaS lectin of the S fimbriae mediates the adherence of meningitis-associated E. coli to sialyl oligosaccharide chains of cFn.
Sarén
A
A
Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland.
Virkola
R
R
Hacker
J
J
Korhonen
T K
TK
D001422:Bacterial Adhesion / Q000502:physiology*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D004927:Escherichia coli Infections / Q000209:etiology* / Q000382:microbiology; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D016920:Meningitis, Bacterial / Q000209:etiology* / Q000382:microbiology; D005810:Multigene Family; D011485:Protein Binding
D002241:Carbohydrates; D005353:Fibronectins