Change in the localization of heat shock protein 27 (HSP 27) in BG-1 human ovarian cancer cells following treatment by the ether lipid ET-18-OCH3.
We have demonstrated a higher nuclear protein content in the hypodiploid fraction of BG-1 human ovarian cancer cells following treatment with one of the ether lipids, ET-18-OCH3. In this study, we have attempted to identify the overexpressed nuclear protein induced in those dying or dead cells in the hypodiploid fraction and its localization before and after ET-18-OCH3 treatment. The pattern of nuclear proteins was analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) before and after ET-18-OCH3 treatment. The partial amino acid sequence of the most dominantly and consistently up-regulated protein spot after ET-18-OCH3 treatment was determined and it was found to be heat shock protein 27 (HSP27). Immunofluorescence staining disclosed that HSP27 localizes in the cytoplasm of the BG-1 cells before ET-18-OCH3 treatment. Condensation of HSP27 around the nuclei was observed following treatment by ET-18-OCH3. Ultimately, the nuclei of the cells in the hypodiploid fraction were stained by immunofluorescent HSP27. These results indicate that change of the localization of HSP27 may play an important role as a component of the signal transduction pathways affected by ether lipids.
Fujiwara
K
K
Department of Obstetrics and Gynecology, Kawasaki Medical School, Kurashiki-City, Japan. fujiwara@med.kawasaki-m.ac.jp
Shirafuji
H
H
Fushitani
K
K
Fujimoto
K
K
Kohno
I
I
Modest
E J
EJ
D000230:Adenocarcinoma / Q000378:metabolism*; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D010051:Ovarian Neoplasms / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010742:Phospholipid Ethers; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D010713:Phosphatidylcholines; D010742:Phospholipid Ethers; C026659:edelfosine