Achiral and chiral determination of tramadol and its metabolites in urine by capillary electrophoresis.
An achiral and chiral separation for the determination of tramadol and its main metabolite O-demethyltramadol in urine samples by CE with UV detection was developed. It was possible to separate tramadol and its phase I and phase II metabolites in one single run using a borate buffer. Furthermore, the simultaneous chiral separation of tramadol and the phase I metabolites was achieved using carboxymethyl-beta-cyclodextrin as chiral selector. To reach the required limits of quantification for the analytes, a preconcentration by solid-phase extraction for the achiral assay and by liquid-liquid extraction for the chiral assay was used. The methods were validated and their applicability was shown by the determination of tramadol and O-demethyltramadol in urine samples.
Kurth
B
B
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D000701:Analgesics, Opioid / Q000652:urine*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D014147:Tramadol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine*
D000701:Analgesics, Opioid; C080580:O-demethyltramadol; D014147:Tramadol