Alteration of N-acetylglucosaminyltransferases in pancreatic carcinoma.
The activities of three N-acetylglucosaminyltransferases (GnT III, GnT IV and GnT V) were determined in 10 samples of pancreatic carcinoma (PCa) and compared with those in 9 samples of normal pancreatic tissue (NP). It was found that the specific activities of GnT III, GnT IV and GnT V increased in all of the PCa samples. GnT III increased most significantly, up to 22.3 fold of normal, GnT IV was elevated 12.3 fold, while GnT V increased only 2.4 fold. The elevation of GnTs in pancreatic carcinoma was consistent with the increase in the number of antenna and bisecting GlcNAc structures in N-glycans of pancreatic ribonuclease (RNase) as assessed by Con A affinity chromatography. Polycytidylate specific RNase from the serum of PCa patients showed the same structural changes as that found in in N-glycans of the RNase from PCa tissue.
Nan
B C
BC
Key Laboratory of Glycoconjugate Research, Ministry of Public Health, Shanghai Medical University, China.
Shao
D M
DM
Chen
H L
HL
Huang
Y
Y
Gu
J X
JX
Zhang
Y B
YB
Wu
Z G
ZG
D000328:Adult; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D010190:Pancreatic Neoplasms / Q000201:enzymology*; D012260:Ribonucleases / Q000097:blood / Q000378:metabolism
D017351:N-Acetylglucosaminyltransferases; D012260:Ribonucleases