[Evaluation of the interactions between strains of S. aureus and peripheral blood phagocytic cells].
The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal evaluation these cells were incubated with suspensions of opsonized bacteria. In parallel, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate the efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocynate (FITC) and the percentage of cells containing FITC-labelled bacteria were analysed by flow cytometry. The data obtained show the strains of S. aureus derived from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes--strains isolated from the blood show the highest, but strains isolated from the respiratory tract have the lowest sensitivity to killing. These strains differ too in their ability to trigger monocyte CL response. On the contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed a significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.
Baran
J
J
Zakład Immunologii Klinicznej i Mikrobiologii, Polsko-Amerykański Instytut Pediatrii Collegium Medicum UJ w Krakowie.
Pajdak
E
E
Isendorf
M
M
Szmyd
D
D
D001769:Blood / Q000382:microbiology; D001772:Blood Cell Count; D005434:Flow Cytometry; D006801:Humans; D008163:Luminescent Measurements; D009000:Monocytes / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000276:immunology; D012137:Respiratory System / Q000382:microbiology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D013492:Suppuration / Q000382:microbiology
D017382:Reactive Oxygen Species