Diet modification affects DNA oxidative damage in healthy humans.
DNA 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a promising biomarker for oxidative damage. We assessed its responsiveness to diet in 32 nonsmoking, healthy subjects (12 male, 20 female) aged 31+/-7.6 years. They consumed two liquid formula diets (Ensures) as the sole source of nutrition for 10-d in a randomized crossover design, with 5-d control solid food diets as washout before each liquid diet period. Reformulated Ensure (Re-En) had a vitamin E/ PUFA of 3.5 compared to standard Ensure (En) of 1.1. We hypothesized that subjects would have lower leukocyte 8-OHdG/deoxyguanosine (dG) ratios while consuming Re-En compared to En. But 8-OHdG/dG ratios did not change with the consumption of either Re-En or En. The mean ratios of 8-OHdG/dG after 10 days of Re-En and En consumption were (2.12+/-0.68)x10(-5) and (2.16+/-0.63)x10(-5), respectively. However, there was a 22% decrease in 8-OHdG/dG by the end of the study and a significant downward trend of leukocyte 8-OHdG among all subjects throughout all nutrient-rich diet phases as the study progressed (Test for trend: p = .04; paired t-test: p = .07). Because all the experimental diets provided antioxidant nutrients at higher quantities than typically consumed by a U.S. age-matched population, this study adds to the few in vivo studies that show a decrease in DNA damage in healthy nonsmoking subjects through dietary intervention.
Chen
L
L
Department of Human Nutrition and Dietetics, University of Illinois at Chicago, 60612, USA.
Bowen
P E
PE
Berzy
D
D
Aryee
F
F
Stacewicz-Sapuntzakis
M
M
Riley
R E
RE
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood; D018592:Cross-Over Studies; D004247:DNA / Q000097:blood*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D004042:Dietary Fats, Unsaturated; D019422:Dietary Sucrose; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D005526:Food, Formulated; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D012044:Regression Analysis; D013997:Time Factors; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
D015415:Biomarkers; D004042:Dietary Fats, Unsaturated; D019422:Dietary Sucrose; C082131:Ensure formulated food; D014810:Vitamin E; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine