Nerve growth factor modulates myelin-associated glycoprotein binding to sensory neurons.
Myelin-associated glycoprotein (MAG) is a molecule expressed by myelinating cells at the myelin/axon interface, which binds to an as yet unidentified molecule on neurons. We have used a MAG-immunoglobulin Fc fusion protein to examine the expression and regulation of the MAG binding molecule on sensory neurons in culture. Binding of the MAG-Fc reached a maximum at 24-48 h and was higher on neurons which expressed high levels of neurofilament. Nerve growth factor (NGF) upregulated expression of the MAG binding molecule in a dose dependent manner. Schwann cells co-cultured with sensory neurons in serum-free medium stimulated maximal expression of the MAG binding molecule, which was decreased by addition of anti-NGF to the co-cultures. This indicated that Schwann cells can modulate expression of the MAG binding molecule via production of NGF and may represent a physiological mechanism for regulation of MAG-MAG binding molecule interactions during myelination and remyelination.
Turnley
A M
AM
The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia. turnley@wehi.edu.au
Bartlett
P F
PF
D000818:Animals; D001369:Axons / Q000378:metabolism; D001665:Binding Sites; D002478:Cells, Cultured; D018920:Coculture Techniques; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000254:growth & development; D006001:Glycoconjugates / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6; D007700:Kinetics; D053605:Leukemia Inhibitory Factor; D008081:Liposomes; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D009186:Myelin Sheath / Q000502:physiology; D018992:Myelin-Associated Glycoprotein / Q000235:genetics / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D016900:Neurofilament Proteins / Q000096:biosynthesis; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism*
D016895:Culture Media, Serum-Free; D006001:Glycoconjugates; D006131:Growth Inhibitors; D007141:Immunoglobulin Fc Fragments; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008081:Liposomes; D008222:Lymphokines; D018992:Myelin-Associated Glycoprotein; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; D011993:Recombinant Fusion Proteins; D019158:N-Acetylneuraminic Acid