title: Content and structure of ceramide and sphingomyelin and sphingomyelinase activity in mouse hepatoma-22.
abstract: The relative content of phosphatidylcholine is lower and that of sphingomyelin is higher in transplantable fast growing mouse hepatoma-22, thus decreasing their ratio approximately 2.5-fold versus normal liver. The ceramide content and the neutral sphingomyelinase activity is markedly higher (3- and 6.5-fold, respectively), whereas the acid sphingomyelinase activity is 4-fold lower in hepatoma-22 versus normal liver. The content of saturated fatty acids in ceramide and sphingomyelin of hepatoma-22 is higher than in normal liver. All sphingolipids of hepatoma-22 contain a considerable amount (25-37%) of sphinganine (dihydrosphingosine) along with sphingenine (sphingosine), whereas sphingolipids of normal liver contain predominantly sphingenine (over 95%). These results indicate that the activity of enzymes involved in sphingolipid biosynthesis and catabolism is disturbed in the transplantable mouse hepatoma-22 compared to normal liver.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117871, Russia. dyatl@ibch.siobc.ras.ru.
authors.forename: S N
authors.initials: SN
authors.lastname: Rylova
authors.forename: O G
authors.initials: OG
authors.lastname: Somova
authors.forename: E S
authors.initials: ES
authors.lastname: Zubova
authors.forename: L B
authors.initials: LB
authors.lastname: Dudnik
authors.forename: L S
authors.initials: LS
authors.lastname: Kogtev
authors.forename: A M
authors.initials: AM
authors.lastname: Kozlov
authors.forename: A V
authors.initials: AV
authors.lastname: Alesenko
authors.forename: E V
authors.initials: EV
authors.lastname: Dyatlovitskaya
mesh_terms: D000818:Animals; D002518:Ceramides / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D015394:Molecular Structure; D011487:Protein Conformation; D013108:Sphingomyelin Phosphodiesterase / Q000737:chemistry / Q000378:metabolism*; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002518:Ceramides; D013109:Sphingomyelins; D013108:Sphingomyelin Phosphodiesterase