title: Exposure to mutagenic airborne particulate in a rubber manufacturing plant.
abstract: Epidemiological studies conducted in the 1980s revealed that people working in the rubber manufacturing industry had an increased risk of cancer. Even now, workers employed in rubber processing are still at risk despite the measures adopted to improve their working conditions. The aim of the study was to evaluate the presence of a genotoxic risk in a rubber industry and to verify whether or not it was possible to locate the most dangerous position among the different rubber-working processes. The mutagenic activity of airborne particulate was evaluated in samples collected in the mixing department of a rubber manufacturing plant. Ambient air samples were taken over 3-h period in two stable positions near the mixing (Banbury mixer) and calendering areas. Personal air samples were taken over 2-h period during a normal workday from five workers employed in different rubber processing operations (mixing, weighing, calendering, compounding and extruding). The mutagenic activity of the air samples was determined by plate incorporation assay using Salmonella typhimurium strains (TA 98, TA 98NR, TA 100, YG 1021) with and without metabolic activation. Polycyclic aromatic hydrocarbon (PAH) concentrations were determined by high-performance liquid chromatography (HPLC); the presence of other presumable contaminants were carried out by gas chromatography-mass spectrometry (GC-MS). The results showed substantial direct and indirect frameshift mutagenicity in both ambient and personal samples. No mutagenic activity was present in S. typhimurium TA 100, except in the personal sample from a worker employed on the Banbury mixer. HPLC analysis revealed very low concentrations of PAHs. GC-MS analysis showed the presence of compounds such as azulene derivative, 1,2-dihydro-2,2,4-trimethylquinoline, N-methyl N-phenylbenzenamine, diphenylamine, bis(2-ethylhexyl)phthalate and bis(methyl-propyl)phthalate. We conclude that the high levels of mutagenic activity in ambiental and personal samples indicate the presence of substances with high genotoxic potency; no substantial differences were seen among the several rubber processing operations. PAHs were not involved in indoor pollution. GC-MS analysis revealed the presence of compounds which may be produced by high temperatures to which the raw materials are subjected during rubber manufacturing processes. These substances are potential carcinogen though their mutagen properties have not been clearly determined.
authors.affiliation: Institute of Pharmacology, Faculty of Medicine, University of Verona, Policlinico Borgo Roma, Verona 37134, Italy. fracasso@farma.univr.it
authors.forename: M E
authors.initials: ME
authors.lastname: Fracasso
authors.forename: P
authors.initials: P
authors.lastname: Franceschetti
authors.forename: E
authors.initials: E
authors.lastname: Mossini
authors.forename: S
authors.initials: S
authors.lastname: Tieghi
authors.forename: L
authors.initials: L
authors.lastname: Perbellini
authors.forename: L
authors.initials: L
authors.lastname: Romeo
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis* / Q000494:pharmacology; D001711:Biotransformation; D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D016368:Frameshift Mutation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007558:Italy; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000494:pharmacology; D016273:Occupational Exposure; D012408:Rubber; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: D000395:Air Pollutants, Occupational; D002273:Carcinogens; D009153:Mutagens; D012408:Rubber