Candida albicans mannan extract-protein conjugates induce a protective immune response against experimental candidiasis.
Candida albicans mannan extracts encapsulated in liposomes were previously used to stimulate mice to produce antibodies protective against candidiasis. In the present study, mannan-protein conjugates without liposomes were tested as vaccine candidates. Mannan extracts were coupled to bovine serum albumin, and isolated conjugates consisted of carbohydrate and protein at a ratio of 0.7-1.0. Vaccination of mice with the conjugate and an adjuvant yielded antiserum that contained Candida agglutinins. Vaccinated mice challenged with yeast cells had a mean survival time of 56 days, compared with <13 days for control groups. The antiserum protected naive animals against disseminated disease. Naive mice given the antiserum intravaginally developed 79% fewer fungal colony-forming units, compared with control groups. The serum-protective factor was stable at 56 degrees C and was removed by adsorption with yeast cells. It is concluded that the conjugate vaccine can induce protective antibody responses against experimental disseminated candidiasis and Candida vaginal infection.
Han
Y
Y
Department of Microbiology, Montana State University, Bozeman, MT 59717-3520, USA.
Ulrich
M A
MA
Cutler
J E
JE
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000918:Antibody Specificity; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control*; D004337:Drug Carriers; D004339:Drug Compounding; D005260:Female; D005657:Fungal Vaccines / Q000276:immunology / Q000627:therapeutic use*; D007116:Immunization, Passive; D018796:Immunoconjugates / Q000276:immunology / Q000627:therapeutic use*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008081:Liposomes; D008351:Mannans / Q000276:immunology / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination
D000908:Antibodies, Fungal; D004337:Drug Carriers; D005657:Fungal Vaccines; D018796:Immunoconjugates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008081:Liposomes; D008351:Mannans