Inhibition of liver metastases from neuraminidase-treated colon 26 cells by an anti-Thomsen-Friedenreich-specific monoclonal antibody.
Thomsen-Friedenreich antigen (TF; Galbeta1-3GalNAcalpha1-) is expressed on many human carcinomas. Evidence suggests that TF-carrying tumor cells specifically bind asialoglycoprotein receptors on hepatocytes resulting in metastasis formation in the liver. We used an animal model to examine the feasibility of preventing metastasis formation by an antibody to TF. Treatment of Colon 26 cells with neuraminidase led to the exposure of TF, and consequently to a higher frequency of liver metastases in syngeneic Balb/c mice. This could be prevented by an antibody to TF (A78-G/A7), but not by a control antibody. The results may open up a new strategy for the prophylaxis of metastatic spread to the liver.
Shigeoka
H
H
First Department of Surgery, Kinki University School of Medicine, Osaka, Japan. shigeoka@surg1.med.kindai.ac.jp
Karsten
U
U
Okuno
K
K
Yasutomi
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D000951:Antigens, Neoplasm / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D007075:Immunoglobulin M / Q000627:therapeutic use*; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control* / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009439:Neuraminidase / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured / Q000637:transplantation
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D007075:Immunoglobulin M; C016467:Thomsen-Friedenreich antigen; D009439:Neuraminidase