Kinetics of thermal inactivation of penaeus penicillatus acid phosphatase
The kinetics of thermal inactivation of Penaeus penicillatus acid phosphatase have been studied using a kinetic method related to the substrate reaction during irreversible inhibition of the enzyme activity as previously described by Tsou (Adv. Enzymol. Relat. Areas Mol. Biol. (1988) 61, 381-436). The kinetics of thermal inactivation of the enzyme show that the reaction is irreversible. The microscopic rate constants were determined for thermal inactivation of free enzyme and the enzyme--substrate complex. The results show that the presence of substrate has a significant protective effect against thermal inactivation of the enzyme.
Yang
P
Department of Biology, Xiamen University, Xiamen, 361005, People's Republic of China.
Chen
Q
Xie
Z
Chen
S
Yang
Y
Park
Y
Zhou
H