title: Outgrowth of pseudopodial cables induced by all-trans retinoic acid in micromere-derived cells isolated from sea urchin embryos.
abstract: Cultured cells derived from micromeres of sea urchin embryos underwent pseudopodial cable growth without spicule rod formation in the presence of all-trans retinoic acid (tRA) or insulin. Pseudopodial cable growth caused by tRA or insulin was inhibited by genistein, a protein tyrosine kinase inhibitor. Phosphorylation of protein tyrosine residue was augmented in the cells treated with tRA or insulin and was inhibited by genistein. Probably, protein tyrosine kinase takes an indispensable part in signal transduction systems for tRA and insulin in these cells. In tRA-treated cells, augmentation of the phosphorylation of protein tyrosine residue was accompanied by an increase in the activity of protein tyrosine kinase and was inhibited by actinomycin D, inhibiting cable growth. Activation of this enzyme in tRA-treated cells probably depends on RNA synthesis. In insulin-treated cells, augmentation of tyrosine residue phosphorylation occurred without any appreciable change in this enzyme's activity and was hardly affected by actinomycin D. Phosphorylation of protein tyrosine residue seems to be activated by the binding of insulin to an insulin receptor. Pseudopodial cable growth in these cells treated with tRA or insulin was inhibited by wortmannin. Phosphatidylinositol 3 kinase probably participates in tRA and insulin signal transduction systems.
authors.affiliation: Department of Biology, School of Education, Waseda University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kuno
authors.forename: M
authors.initials: M
authors.lastname: Kawamoto
authors.forename: M
authors.initials: M
authors.lastname: Okuyama
authors.forename: I
authors.initials: I
authors.lastname: Yasumasu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D010766:Phosphorylation; D012617:Sea Urchins / Q000196:embryology*; D015398:Signal Transduction; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D007328:Insulin; D014212:Tretinoin