title: Maize glutathione-dependent formaldehyde dehydrogenase: protein sequence and catalytic properties.
abstract: Glutathione-dependent formaldehyde dehydrogenase (FDH; EC 1.2.1.1) has been purified 3900-fold from maize cell-suspension cultures to a specific activity of 4.68 mumol (mg protein)-1 min-1. The homogeneous enzyme consisted of two identical subunits with a molecular mass of 42 kDa, and an isoelectric point of 5.8. Eight tryptic peptides were sequenced and gave a perfect fit to the protein sequence derived from maize Fdh cDNA (J. Fliegmann and H. Sandermann, 1997, Plant Mol Biol 34: 843-854). There was 62% identity with the eucaryotic FDH consensus sequence. Michaelis constants of approx. 20 microns (formaldehyde), approx. 50 microns (glutathione) and approx. 31 microns (NAD+) were determined for the maize enzyme as well as for FDH partially purified from dog lung. Besides S-hydroxymethylglutathione, pentanol-1, octanol-1, and omega-hydroxy-fatty acids served as substrates for both FDH preparations. The unusual substrate specificity indicates that FDH may be involved in the detoxification of long-chain lipid peroxidation products.
authors.affiliation: GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Biochemische Pflanzenpathologie, Oberschleissheim, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Wippermann
authors.forename: J
authors.initials: J
authors.lastname: Fliegmann
authors.forename: G
authors.initials: G
authors.lastname: Bauw
authors.forename: C
authors.initials: C
authors.lastname: Langebartels
authors.forename: K
authors.initials: K
authors.lastname: Maier
authors.forename: H
authors.initials: H
authors.lastname: Sandermann
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D005978:Glutathione / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000201:enzymology*
chemical_list: D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D005978:Glutathione