A monoclonal antibody-blocking enzyme-linked immunosorbent assay for the detection of serovar-specific antibodies to Haemophilus paragallinarum.
Two monoclonal antibody-blocking enzyme-linked immunosorbent assays (B-ELISAs) were developed to detect serovar-specific antibodies to Haemophilus paragallinarum. One assay detected antibodies against serovar A and the other antibodies against serovar C. The assays were evaluated with sera derived from disease-free chickens as well as chickens experimentally immunized and/or challenged with H. paragallinarum strains 0083 (serovar A), Modesto (serovar C), or HP31 (serovar C). When tested with 440 negative sera (170 from a specific-pathogen-free and 30 from each of nine commercial layer flocks), both tests gave only a single false-positive reaction. The use of the B-ELISAs with the experimentally produced sera showed the assays to be serovar specific. With the exception of one serum, the serovar A B-ELISA detected antibodies only in the chickens vaccinated with 0083. Similarly, with the exception of one serum, the serovar C B-ELISA detected antibodies only in those chickens vaccinated with Modesto or those chickens challenged with HP31. Overall, the serovar A B-ELISA had a specificity of 99.7% and a sensitivity of 78.7%, whereas the serovar C B-ELISA had a specificity of 99.8% and a sensitivity of 64.7%.
Zhang
P
P
Institute for Animal Husbandry and Veterinary Science, Beijing Academy of Agricultural and Forestry Science, Peoples Republic of China.
Blackall
P J
PJ
Yamaguchi
T
T
Iritani
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006190:Haemophilus / Q000145:classification / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal