Characterization and hormonal modulation of immunoreactive thiamin carrier protein in immature rat Sertoli cells in culture.
Immature rat Sertoli cells synthesize and secrete a protein species which has immunological similarity with chicken egg thiamin carrier protein (TCP) as assessed by immunocytochemical localization, liquid phase radioimmunoassay (RIA), immunoprecipitation of [35S]-methionine incorporated newly synthesized proteins by polyclonal antibodies (pAbs) to chicken TCP and tryptic peptide mapping of iodinated immunoprecipitated proteins. FSH and testosterone together bring about 4-fold induction of Sertoli cell TCP over the control levels which is inhibitable upto 75% by an aromatase inhibitor. Addition of optimal concentrations of exogenous estradiol-17beta to the cultures causes 2-fold enhancement of secretion of TCP which can significantly be inhibited by tamoxifen, when added along with estradiol-17beta. These results show that Sertoli cells produce estrogen-inducible TCP, presumably to transport the vitamin to the developing germ cells.
Subramanian
S
S
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore.
Adiga
P R
PR
D000818:Animals; D047072:Aromatase Inhibitors; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*; D013629:Tamoxifen / Q000494:pharmacology; D013737:Testis / Q000166:cytology / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology; D013831:Thiamine / Q000378:metabolism*
D047072:Aromatase Inhibitors; D002352:Carrier Proteins; D004791:Enzyme Inhibitors; D004965:Estrogen Antagonists; D006728:Hormones; D013629:Tamoxifen; D013739:Testosterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D013831:Thiamine