title: Phenotypic characteristics of B cells in Behçet's disease: increased activity in B cell subsets.
abstract: OBJECTIVE
  Increased numbers of spontaneous Ig secreting B cells and elevated immunoglobulin levels have been described in Behçet's disease (BD), in addition to changes in numbers and activities of T cells, natural killer cells, and monocyte-macrophages. We investigated other characteristics of B cells in BD.
  
  
  METHODS
  B lymphocyte subsets (CD19+CD5+, CD19+CD13+, CD19+CD28+, CD19+CD33+, CD19+CD80+, CD5+CD19+CD45RA+, CD5+CD19+CD45RO+) were phenotypically evaluated in 50 patients with BD, 80 healthy subjects, and 20 other patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and sepsis.
  
  
  RESULTS
  Although the B cell number (CD19+) was normal, CD13 and CD33 positive B cells were more numerous in BD and sepsis compared to healthy controls and patients with RA and SLE. The percentage of CD45RO positive B cells was higher in both BD and sepsis, while the percentage of CD80 positive B cells was high only in BD. There was no increase in the CD5+CD19+ B cell subset, previously shown to be increased in several autoimmune diseases. Naive (CD45RA) and memory (CD45RO) status of CD5+CD19+ and CD5-CD19+ B cells showed that CD45RA expression was higher in CD5+CD19+ B cells, whereas expression of both CD45RA and CD45RO was higher in the CD5-CD19+ B cell group compared with healthy controls.
  
  
  CONCLUSIONS
  Although the total B cell number was normal, increased levels of activated and memory B cell subsets suggest a modified B cell function in BD, which may be related to a weak stimulus by an unknown external antigen.
authors.affiliation: Department of Hematology-Immunology, Marmara University School of Medicine, Kadiköy, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Ekşioglu-Demiralp
authors.forename: A
authors.initials: A
authors.lastname: Kibaroglu
authors.forename: H
authors.initials: H
authors.lastname: Direskeneli
authors.forename: S
authors.initials: S
authors.lastname: Yavuz
authors.forename: F
authors.initials: F
authors.lastname: Karsli
authors.forename: S
authors.initials: S
authors.lastname: Yurdakul
authors.forename: H
authors.initials: H
authors.lastname: Yazici
authors.forename: T
authors.initials: T
authors.lastname: Akoglu
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D001528:Behcet Syndrome / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000276:immunology / Q000378:metabolism
chemical_list: D015703:Antigens, CD