title: MR imaging of the arthritic rabbit knee joint using albumin-(Gd-DTPA)30 with correlation to histopathology.
abstract: The purpose of this study was to demonstrate a technique, in a pilot study, for measuring abnormal capillary permeability in synovial tissue of rabbit arthritic knees using dynamic MRI with a gadolinium-based blood pool agent. Arthritis, simulating rheumatoid arthritis, was induced in knees of 8 rabbits by intra-articular injection of carrageenan (n = 4) or ovalbumin (n = 4). Sequential fat presaturated T1-weighted Spoiled Grass images were obtained before and up to 30 min after intravenous administration of albumin-(Gd-DTPA)30. Estimates of synovial tissue plasma-volume (PV), fractional-leak-rate (FLR), and permeability-surface-area-product (PS) were computed. Histologic correlation was obtained in the corresponding regions. Dynamic MRI showed extravasation of albumin-(Gd-DTPA)30 into hypertrophic synovium in six of the eight arthritic knees. Histologic examination of these six knees showed markedly inflamed synovium. The two knees that did not show abnormal vascular permeability contained non-hypertrophic synovium. None of the rabbits showed abnormal permeability in muscle. MRI derived microvascular characteristics (PV, FLR and PS) correlated positively (r2 = 0.51, 0.97 and 0.86) with the histology. Factors involving the structural and functional microvascular characteristics of synovial tissue can be estimated non-invasively using albumin-(Gd-DTPA)30. This technique may be useful for monitoring disease progression and treatment response in rheumatoid arthritis.
authors.affiliation: Department of Radiology, University of California, San Francisco 94143-0628, USA.
authors.forename: C F
authors.initials: CF
authors.lastname: van Dijke
authors.forename: C G
authors.initials: CG
authors.lastname: Peterfy
authors.forename: R C
authors.initials: RC
authors.lastname: Brasch
authors.forename: P
authors.initials: P
authors.lastname: Lang
authors.forename: T P
authors.initials: TP
authors.lastname: Roberts
authors.forename: D
authors.initials: D
authors.lastname: Shames
authors.forename: J B
authors.initials: JB
authors.lastname: Kneeland
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: J S
authors.initials: JS
authors.lastname: Mann
authors.forename: S D
authors.initials: SD
authors.lastname: Kapila
authors.forename: H K
authors.initials: HK
authors.lastname: Genant
mesh_terms: D000418:Albumins; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D002199:Capillary Permeability; D002351:Carrageenan; D003287:Contrast Media; D019786:Gadolinium DTPA; D007091:Image Processing, Computer-Assisted; D007719:Knee Joint / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010047:Ovalbumin; D010865:Pilot Projects; D011817:Rabbits; D013583:Synovial Membrane / Q000098:blood supply / Q000378:metabolism / Q000473:pathology
chemical_list: D000418:Albumins; D003287:Contrast Media; C058946:albumin-(gadolinium-DTPA); D002351:Carrageenan; D010047:Ovalbumin; D019786:Gadolinium DTPA