title: Isolation of peptides from an enzymatic hydrolysate of food proteins and characterization of their taste properties.
abstract: Soybean protein, casein, bonito protein and chicken protein, each as foodstuff protein, were hydrolyzed with four proteinases; namely, pepsin, trypsin, alpha-chymotrypsin and bromelain. Since the chicken protein hydrolysate with bromelain possessed the most favorable umami taste, eleven peptides were isolated from the chicken protein hydrolysate by successive chromatography on ODS, Amberlite IR-120B, Amberlite IRA-410 and AG-50W; their structures were Asp-Ala, Asp-Val, Glu-Glu, Glu-Val, Ala-Asp-Glu, Ala-Glu-Asp, Asp-Glu-Glu, Asp-Glu-Ser, Glu-Glu-Asn, Ser-Pro-Glu, and Glu-Pro-Ala-Asp. Many of them did not show any umami taste by themselves, but Glu-Glu, Glu-Val, Ala-Asp-Glu, Ala-Glu-Asp, Asp-Glu-Glu, and Ser-Pro-Glu were recognized to enhance the umami taste of 0.02% 5'-inosine monophosphate (IMP). A combination of these peptides, especially 0.5% each of Glu-Glu, Glu-Val, Asp-Glu-Glu and Glu-Glu-Asn, with 0.02% IMP produced a delicious "full" umami taste.
authors.affiliation: Department of Fermentation Science, Tokyo University of Agriculture, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maehashi
authors.forename: M
authors.initials: M
authors.lastname: Matsuzaki
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Udaka
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004044:Dietary Proteins / Q000032:analysis*; D010450:Endopeptidases / Q000737:chemistry; D005399:Fishes; D006801:Humans; D008460:Meat / Q000032:analysis; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry; D013649:Taste
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D030262:Soybean Proteins; D010450:Endopeptidases