Endothelial dysfunction in DOCA-salt hypertension: possible involvement of prostaglandin endoperoxides.
The effects of the arachidonic acid metabolism inhibitors on the acetylcholine responses of aortae from control (CR) and deoxycorticosterone acetate (DOCA)-salt hypertensive (HR) rats were investigated. The acetylcholine decreased response observed in HR [relaxation (%): CR 95.5+/-2.7, n = 4; HR 52.0+/-6.3, n = 5, p < 0.05] was restored by the cyclooxygenase inhibitor piroxicam [relaxation (%): CR 99.8+/-0.2, n = 4; HR 86.0+/-4.0, n = 5] and by the thromboxane synthetase inhibitor and the thromboxane A2/prostaglandin H2 receptor antagonist ridogrel [relaxation (%): CR 92.1+/-4.4, n = 7; HR 93.1+/-2.0, n = 7] but not by the inhibitors of thromboxane synthetase, prostacyclin synthetase, cytochrome P-450 monooxygenase, and lipoxygenase. So, endoperoxide intermediates seem to be involved in the decreased endothelium-dependent relaxation to acetylcholine in DOCA-salt hypertension.
Cordellini
S
S
Department of Pharmacology, Institute of Biosciences, University Estadual Paulista, São Paulo, Brazil. cordelli@ibb.unesp.br
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D016718:Arachidonic Acid / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D003900:Desoxycorticosterone; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000503:physiopathology; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D010894:Piroxicam / Q000494:pharmacology; D011449:Prostaglandin Endoperoxides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation
D016861:Cyclooxygenase Inhibitors; D016232:Endothelins; D011449:Prostaglandin Endoperoxides; D010894:Piroxicam; D016718:Arachidonic Acid; D003900:Desoxycorticosterone; D000109:Acetylcholine