The effect of mitomycin-C on the healing process in rabbit extraocular muscle surgery.
OBJECTIVE
One of the most serious complications following extraocular muscle surgery is formation of postoperative adhesions that may result in restrictive ocular motility dysfunction. The effect of mitomycin-C (MMC) on postoperative wound healing in extraocular muscle surgery was investigated.


METHODS
Sixteen albino rabbits (24 eyes) were used and anesthetized with ketamine and xylazine. These rabbits were divided into 3 groups; (1) control group (balanced salt solution (BSS) exposure for 5 minutes), (2) Group 1 (0.5 mg/ml MMC exposure for 1 minute) and (3) Group 2 (0.5 mg/ml MMC exposure for 5 minutes). After 3 mm recession of superior rectus muscle, MMC or BSS was applied using Weck-Cel sponge (Solan, Jacksonville, FL). The reduction of fibroblast and collagen fiber proliferation were evaluated histopathologically with light microscope at 1, 2, 4 and 8 weeks after operation. We also evaluated the amount of basic fibroblast growth factor (bFGF) by immunohistochemical study at the same time.


RESULTS
Histopathologically, there was a more significant reduction of fibroblast and collagen fiber proliferation in Group 1 and 2 than the control group. Immunohistochemically, Group 1 and 2 showed lesser expression of bFGF than the control group. But, there was no difference between Group 1 and 2.


CONCLUSIONS
Topical intraoperative application of mitomycin C in extraocular muscle surgery may reduce postoperative adhesions.
Oh
S Y
SY
Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
Park
D W
DW
Chang
B L
BL
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005500:Follow-Up Studies; D016685:Mitomycin / Q000494:pharmacology*; D015835:Ocular Motility Disorders / Q000601:surgery; D009801:Oculomotor Muscles / Q000601:surgery*; D013508:Ophthalmologic Surgical Procedures; D011817:Rabbits; D012590:Sclera / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000267:Tissue Adhesions / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
D016222:Fibroblast Growth Factor 2; D016685:Mitomycin