title: Intratumor heterogeneity of chromosome 1, 7, 17, and 18 aneusomies obtained by FISH and association with flow cytometric DNA index in human colorectal adenocarcinomas.
abstract: BACKGROUND
  The origin and evolution of somatic chromosome aberrations in colorectal cancer is still poorly understood. The data in the literature suggest that some specific chromosome aberrations are more common. It is not known, however, if there is a correlation of these with near-diploid and high aneuploidy previously proposed to be a characteristic of the adenoma-carcinoma sequence.
  
  
  METHODS
  Chromosome 1, 7, 17 and 18 numerical aberrations and 1p deletions were evaluated by fluorescence in situ hybridization analysis for 20 human sporadic colorectal adenocarcinomas in 70 distinct tumor sectors and correlated with flow cytometric DNA index (DI) values.
  
  
  RESULTS
  Aneusomy for at least one of the investigated chromosomes was observed in 60 of 70 tumor sectors corresponding to 19 of 20 adenocarcinomas (95%). Deletions at 1p, observed in 8 of 18 adenocarcinomas (44%), were intratumor homogeneous in 7 of 8 tumors. In contrast, the other aberrations were intratumor heterogeneous. Aneusomies of chromosomes 1, 7, and 17 were strongly associated with DNA high aneuploidy (DI > or = 1.4), whereas aneusomy of chromosome 18 and 1p deletions were equally common among DNA diploid and near-diploid tumors (DI < 1.4 and DI not equal to 1).
  
  
  CONCLUSIONS
  Overall, these data suggest the existence of different aneuploidization routes correlated with specific chromosome aberrations. In addition, intratumor homogeneity of 1p deletions appears to be an indication of early occurrence or strong selection. We also suggest that tumors with monosomies and in particular monosomies-trisomies for the same chromosomes support a model of aneuploidization and chromosome instability during the colorectal tumor progression based on loss of symmetry during chromosome segregation (Giaretti: Lab Invest 71:904-910, 1994).
authors.affiliation: Laboratory of Biophysics and Cytometry, National Cancer Institute, Genoa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Di Vinci
authors.forename: E
authors.initials: E
authors.lastname: Infusini
authors.forename: C
authors.initials: C
authors.lastname: Peveri
authors.forename: A
authors.initials: A
authors.lastname: Sciutto
authors.forename: R
authors.initials: R
authors.lastname: Orecchia
authors.forename: E
authors.initials: E
authors.lastname: Geido
authors.forename: R
authors.initials: R
authors.lastname: Monaco
authors.forename: W
authors.initials: W
authors.lastname: Giaretti
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000782:Aneuploidy; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002887:Chromosomes, Human, Pair 18; D002897:Chromosomes, Human, Pair 7; D015179:Colorectal Neoplasms / Q000235:genetics*; D018740:Genetic Heterogeneity; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*