title: Secondary structure and oligomerization behavior of equilibrium unfolding intermediates of the lambda cro repressor.
abstract: The thermal unfolding of the wild-type Cro repressor, its disulfide-bridged mutant Cro-V55C (with the Val-55 --> Cys single amino acid substitution), and a CNBr-fragment (13-66)2 of Cro-V55C was studied by Fourier transform infrared spectroscopy and dynamic light scattering. The combined approach reveals that thermal denaturation of Cro-WT and Cro-V55C proceeds in two steps through equilibrium unfolding intermediates. The first thermal transition of the Cro-V55C dimer involves the melting of the alpha-helices and the short beta-strand localized in the N-terminal part of the molecule. This event is accompanied by the formation of tetramers, and also impacts on the hydrogen-bonding interactions of the C-terminal beta-strands. The beta-sheet formed by the C-terminal parts of each polypeptide chain is the major structural feature of the intermediate state of Cro-V55C and unfolds during a second thermal transition, which is accompanied by the dissociation of the tetramers. Cutting of 12 amino acids in the N-terminal region is sufficient to prevent the formation of alpha-helical structure in the CNBr-fragment of Cro-V55C, and to induce tetramerization already at room temperature. The tetramers may persist over a broad temperature range, and start to dissociate only upon thermal unfolding of the beta-sheet structure formed by the C-terminal regions. The wild-type protein is a dimer at room temperature and at protein concentrations of 1.8-5.8 mg/mL. At lower concentrations, the dimers are stable until the onset of thermal unfolding, which is accompanied by the dissociation of the dimers into monomers. At higher protein concentrations, the unfolding is more complex and involves the formation of tetramers at intermediate temperatures. At these intermediate temperatures, the Cro-WT has lost all of its alpha-helical structure and also most of its native beta-sheet structure. Upon further temperature increase, a tendency for an intermolecular association of the beta-strands is observed, which may result in irreversible beta-aggregation at high protein concentrations.
authors.affiliation: Max-Delbrück-Center for Molecular Medicine, Berlin, Germany. fabian@mdc-berlin.de
authors.forename: H
authors.initials: H
authors.lastname: Fabian
authors.forename: K
authors.initials: K
authors.lastname: Fälber
authors.forename: K
authors.initials: K
authors.lastname: Gast
authors.forename: D
authors.initials: D
authors.lastname: Reinstädler
authors.forename: V V
authors.initials: VV
authors.lastname: Rogov
authors.forename: D
authors.initials: D
authors.lastname: Naumann
authors.forename: D F
authors.initials: DF
authors.lastname: Zamyatkin
authors.forename: V V
authors.initials: VV
authors.lastname: Filimonov
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D010582:Bacteriophage lambda / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D003488:Cyanogen Bromide; D004268:DNA-Binding Proteins; D004058:Diffusion; D006358:Hot Temperature; D008027:Light; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014633:Valine / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D014633:Valine; D003488:Cyanogen Bromide