title: [Use of protein-polysaccharide Brucella antigen labeled with colloidal gold for detection of specific antibodies by the dot-immunoassay method].
abstract: Antibrucella antibodies were detected by dot-immunoassay with colloid gold label of antigen. The specific antigen was protein-polysaccharide complex (PPC) isolated from vaccine strain Brucella abortus 19 BA by acetic acid hydrolysis of bacterial cells. Dot immunoassay with PPC labeled with colloid gold was effective in testing cattle, rabbit, and human sera for antibrucella antibodies. The proposed test system is simple, economic, highly sensitive and specific, and requires no expensive equipment and reagents, which are its advantages over routine serological tests.
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Zagoskina
authors.forename: A I
authors.initials: AI
authors.lastname: Kalinovskiĭ
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Markov
authors.forename: N L
authors.initials: NL
authors.lastname: Esina
authors.forename: E P
authors.initials: EP
authors.lastname: Golubinskiĭ
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D017979:Gold Colloid / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017979:Gold Colloid; D011135:Polysaccharides, Bacterial