title: Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae.
abstract: Pyruvate-decarboxylase (Pdc)-negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose-containing complex media, but not on defined glucose-containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cerevisiae does not exhibit prolonged growth on glucose. Pdc- strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA.
authors.affiliation: Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, The Netherlands.
authors.forename: M T
authors.initials: MT
authors.lastname: Flikweert
authors.forename: M
authors.initials: M
authors.lastname: de Swaaf
authors.forename: J P
authors.initials: JP
authors.lastname: van Dijken
authors.forename: J T
authors.initials: JT
authors.lastname: Pronk
mesh_terms: D000085:Acetates / Q000378:metabolism; D000105:Acetyl Coenzyme A / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D008962:Models, Theoretical; D011767:Pyruvate Decarboxylase / Q000172:deficiency*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development*
chemical_list: D000085:Acetates; D003470:Culture Media; D000105:Acetyl Coenzyme A; D011767:Pyruvate Decarboxylase; D005947:Glucose