The relation between degranulation and rapid metabolic responses in RBL-2H3 cells.
The relation between degranulation and rapid metabolic responses (acidification rate changes) in RBL-2H3 cells was studied using a cytosensor microphysiometer, a silicon-based biosensor system. The metabolic responses in RBL-2H3 cells by antigen stimulation were compared with those by inhibitors of Ca2+ -ATPase. The former resulted in a rapid transient increase in the acidification rate of RBL-2H3 cells while the latter resulted in gradual decreases. When RBL-2H3 cells were costimulated with the inhibitors of Ca2+ -ATPase and an activator (PMA, phorbol-12-myristoyl-13-acetate) of protein kinase C, the metabolic responses increased again in RBL-2H3 cells. This seems to indicate that degranulation in RBL-2H3 cells was accelerated by costimulation with the inhibitors and PMA. However, costimulation was not able to completely mimic the way antigen stimulated RBL-2H3 cells in degranulation and in rapid metabolic responses.
Furuno
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Ohyama
N
N
Nakanishi
M
M
D000818:Animals; D015549:Basophil Degranulation Test; D001491:Basophils / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D015550:Cell Degranulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D004791:Enzyme Inhibitors; D001619:beta-N-Acetylhexosaminidases; D000252:Calcium-Transporting ATPases; D002118:Calcium