title: An scFv phage clone that binds to human beta2-microglobulin.
abstract: Using phage display technology, a novel mouse scFv phage clone D1 was generated from mice immunized with single-chain HLA-A2, the human MHC class I molecules. D1 recognized a determinant in beta2-microglobulin. It reacted with beta2-microglobulin associated with the heavy chain of HLA-A2, recombinant beta2-microglobulin made in Escherichia coli or beta2-microglobulin prepared from human urine. The heavy chain variable region of D1 belongs to gene family VII and the light chain variable region belongs to the kappa chain gene family XI according to the Kabat database.
authors.affiliation: Laboratory of Molecular Biology, DBS, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: B
authors.initials: B
authors.lastname: Jwang
authors.forename: N
authors.initials: N
authors.lastname: Yerushalmi
authors.forename: R J
authors.initials: RJ
authors.lastname: Kreitman
authors.forename: J
authors.initials: J
authors.lastname: Engberg
authors.forename: I
authors.initials: I
authors.lastname: Pastan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D015789:HLA-A2 Antigen / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D007128:Immunoglobulin Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology*
chemical_list: D015789:HLA-A2 Antigen; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin; C055966:immunoglobulin Fv