title: Absence of limiting amino acids in calves fed a corn and soybean meal diet past three months of age.
abstract: We conducted three nitrogen balance trials using Holstein bull calves older than 16 wk (Trial 1; n = 8), 13 wk (Trial 2; n = 6), and 15 wk of age (Trial 3; n = 9) in a 4 x 4 (Trial 1) or 3 x 3 Latin square design (Trials 2 and 3) to identify limiting amino acids for a corn and soybean meal diet. All calves were trained to maintain reflex closure of the reticular groove after weaning at 5 wk of age. The basal diet was fed daily at 20 or 27 g/kg BW (Trial 1) and at 20 g/kg BW (Trials 2 and 3). The lower feeding level resulted in reduced urinary excretion of purine derivatives, suggesting reduced synthesis of ruminal microbial protein (Trial 1). In Trials 1 and 2, administration of DL-methionine plus L-lysine monohydrochloride through the reticular groove did not increase N retention compared with the supplement of isonitrogenous L-glutamine at either level of intake. In Trial 3, administration of either casein or isonitrogenous monosodium glutamate increased N retention to a similar extent above that observed with a N-free supplement. Results suggested that no specific amino acids were limiting for the corn-soybean meal diet. Administration of methionine plus lysine resulted in a remarkable increase in plasma methionine (Trials 1 and 2), especially at the lower intake level (Trial 1), and a decrease in plasma branched-chain amino acids at either intake level. Glutamine supplementation did not increase plasma branched-chain amino acids compared with the supplementation of diammonium citrate (Trial 2).
authors.affiliation: School of Veterinary Medicine, Azabu University, Sagamihara, Japan. abe@azabu-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Kasahara
authors.forename: T
authors.initials: T
authors.lastname: Iriki
authors.forename: R
authors.initials: R
authors.lastname: Kuriyama
authors.forename: M
authors.initials: M
authors.lastname: Funaba
mesh_terms: D000821:Animal Feed; D000818:Animals; D002417:Cattle / Q000254:growth & development*; D008239:Lysine / Q000032:analysis*; D008297:Male; D008715:Methionine / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D013997:Time Factors; D003313:Zea mays / Q000737:chemistry*
chemical_list: D008715:Methionine; D008239:Lysine