title: Examination of co-complexes for radiolabeling of platelets in positron emission tomographic studies.
abstract: Oxine, tropolonate, and mercaptopyridine-N-oxide (MPO) have been widely used with 111In for platelet labeling. The use of positron emission tomography (PET) radioisotopes for platelet labeling would offer a higher sensitivity and resolution over conventional imaging. The medium half-life PET radioisotope 55Co (t1/2 = 18.2 hours) would enable quantitative uptake and cell kinetic studies with radiolabeled platelets. To identify the optimal complex for radiolabeling of platelets with 55Co, we investigated the platelet uptake of 57Co-oxine, 57Co-tropolonate, and 57Co-MPO in varying different parameters. The platelet uptake of Co-complexes was generally low (5-12%), dependent on time and temperature of incubation and density of platelets but independent of the amount of radioactivity. These findings are not promising for potential PET application.
authors.affiliation: Department of Nuclear Medicine, University of Vienna, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Karanikas
authors.forename: J
authors.initials: J
authors.lastname: Schmaljohann
authors.forename: M
authors.initials: M
authors.lastname: Rodrigues
authors.forename: F
authors.initials: F
authors.lastname: Chehne
authors.forename: S
authors.initials: S
authors.lastname: Granegger
authors.forename: H
authors.initials: H
authors.lastname: Sinzinger
mesh_terms: D000328:Adult; D001161:Arteriosclerosis / Q000097:blood / Q000000981:diagnostic imaging; D001693:Biological Transport, Active; D001792:Blood Platelets / Q000378:metabolism*; D003037:Cobalt Radioisotopes / Q000097:blood / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008297:Male; D019275:Radiopharmaceuticals / Q000097:blood / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013927:Thrombosis / Q000097:blood / Q000000981:diagnostic imaging; D014055:Tomography, Emission-Computed / Q000379:methods*
chemical_list: D003037:Cobalt Radioisotopes; D019275:Radiopharmaceuticals