title: Cutting edge: Fc receptor type I for IgG on macrophages and complement mediate the inflammatory response in immune complex peritonitis.
abstract: The contributions of Fc receptors (FcRs) for IgG (FcgammaRs) and complement to immune complex (IC)-mediated peritonitis were evaluated in BALB/c-, C57BL/6-, FcRgamma chain-, and FcR type III for IgG (FcgammaRIII)-deficient mice, backcrossed to the C57BL/6 background. In BALB/c mice, but not in C57BL/6 mice, neutrophil migration was markedly attenuated after complement depletion. In mice lacking FcRgamma chain, neutrophil migration was abolished, whereas it was unaffected in FcgammaRIII-deficient mice. Huge amounts of TNF-alpha (TNF) were found in the peritoneal exudate of BALB/c and C57BL/6 mice but were absent in mice lacking FcRgamma chain or FcgammaRIII. Surprisingly, a functional inhibition of TNF in BALB/c and C57BL/6 mice had no effect on neutrophil infiltration. These data provide evidence that in IC peritonitis, the activation of FcR type I for IgG on peritoneal macrophages and the activation of the complement cascade, but not the interaction of ICs with FcgammaRIII and the subsequent release of TNF, initiate the inflammatory response in BALB/c and C57BL/6 mice.
authors.affiliation: Institute of Medical Microbiology, and Department of Clinical Immunology, Medical School Hannover, Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Heller
authors.forename: J E
authors.initials: JE
authors.lastname: Gessner
authors.forename: R E
authors.initials: RE
authors.lastname: Schmidt
authors.forename: A
authors.initials: A
authors.lastname: Klos
authors.forename: W
authors.initials: W
authors.lastname: Bautsch
authors.forename: J
authors.initials: J
authors.lastname: Köhl
mesh_terms: D000818:Animals; D001183:Arthus Reaction / Q000235:genetics / Q000276:immunology*; D001202:Ascitic Fluid / Q000737:chemistry / Q000166:cytology; D002634:Chemotaxis, Leukocyte; D003165:Complement System Proteins / Q000276:immunology*; D003433:Crosses, Genetic; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009504:Neutrophils / Q000276:immunology; D010538:Peritonitis / Q000235:genetics / Q000276:immunology*; D017452:Receptors, IgG / Q000276:immunology*; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D000097625:HLA-E Antigens
chemical_list: D003165:Complement System Proteins; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D000097625:HLA-E Antigens