title: Immunogenicity of peptides derived from a fibronectin-binding protein of S. aureus expressed on two different plant viruses.
abstract: The D2 peptide derived from an S. aureus fibronectin-binding protein (FnBP) was expressed on the surface of the icosahedral cowpea mosaic virus (amino acids 1-30 of D2) or on the rod-shaped potato virus X (amino acids 1-38 of D2), termed CPMV-MAST1 and PVX-MAST8, respectively. Mice and rats were immunized subcutaneously with CPMV-MAST1 and mice with PVX-MAST8 in adjuvant and high titres of FnBP-specific antibody were obtained. The mouse IgG was predominantly of the IgG2a and IgG2b isotypes, which strongly bound complement component C1q, suggesting a TH1-bias in the peptide-specific responses. Sera from mice and rats immunized with CPMV-MAST1 and from mice immunized with PVX-MAST8 were shown to completely inhibit the binding of fibronectin to immobilised recombinant FnBP and rat sera against CPMV-MAST1 were able to block adherence of S. aureus to fibronectin. These studies demonstrate that the D2 peptide is highly immunogenic when expressed on 2 different plant viruses and highlight the potential of plant virus-based vaccines to protect against S. aureus infections.
authors.affiliation: Axis Genetics plc, Babraham, Cambridge, UK.
authors.forename: F R
authors.initials: FR
authors.lastname: Brennan
authors.forename: T D
authors.initials: TD
authors.lastname: Jones
authors.forename: M
authors.initials: M
authors.lastname: Longstaff
authors.forename: S
authors.initials: S
authors.lastname: Chapman
authors.forename: T
authors.initials: T
authors.lastname: Bellaby
authors.forename: H
authors.initials: H
authors.lastname: Smith
authors.forename: F
authors.initials: F
authors.lastname: Xu
authors.forename: W D
authors.initials: WD
authors.lastname: Hamilton
authors.forename: J I
authors.initials: JI
authors.lastname: Flock
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017798:Comovirus / Q000235:genetics; D015922:Complement C1q / Q000276:immunology / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D010942:Plant Viruses / Q000235:genetics; D017863:Potexvirus / Q000235:genetics; D011485:Protein Binding; D051381:Rats; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D005353:Fibronectins; D007074:Immunoglobulin G; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; C078635:fibronectin-binding proteins, bacterial; D015922:Complement C1q