title: An animal virus-derived peptide switches membrane morphology: possible relevance to nodaviral transfection processes.
abstract: The N-terminal domain of the capsid protein cleavage product of the flock house virus (FHV) consists of 21 residues and forms an amphipathic alpha-helix, which is thought to play a crucial role in permeabilizing biological membranes for RNA translocation in the host cell. We have found that the Met --> Nle variant of this domain (denoted here as gamma1) efficiently induces the formation of the interdigitated gel phase (LbetaI) of 1, 2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) bilayers. In situ scanning force microscopy of solid supported bilayers and fluorescence spectroscopy of peptide-treated DPPC vesicles provide evidence for the formation of acyl chain interdigitated lipid domains. It could be shown by fluorescence spectroscopy that the peptide inserts in the DPPC matrix above the main transition temperature of the lipid, while the formation of domains with decreased thickness occurs after the sample is cooled to 25 degrees C. The orientation and secondary structure of the peptide in lipid bilayers were investigated using attenuated total reflectance infrared (ATR-IR) and circular dichroism (CD) spectroscopy. These results enabled us to formulate a mechanistic model for the peptide-mediated induction of interdigitation in DPPC bilayers. Moreover, the membrane activity of gamma1 with gel phase lipids established in this study may have further implications for the infection strategy adopted by simple RNA viruses.
authors.affiliation: Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: A
authors.initials: A
authors.lastname: Janshoff
authors.forename: D T
authors.initials: DT
authors.lastname: Bong
authors.forename: C
authors.initials: C
authors.lastname: Steinem
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
authors.forename: M R
authors.initials: MR
authors.lastname: Ghadiri
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000595:Amino Acid Sequence; D002463:Cell Membrane Permeability / Q000235:genetics*; D007304:Insect Viruses / Q000235:genetics / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014162:Transfection; D014364:Tryptophan / Q000737:chemistry; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D010446:Peptide Fragments; D014764:Viral Proteins; D015060:1,2-Dipalmitoylphosphatidylcholine; D014364:Tryptophan