title: The synthetic kappa-opioid agonist (-)U50,488 does not affect calcium transport into R1.1 mouse thymoma cell line.
abstract: In this paper, the effect of the synthetic kappa-opioid agonist (-)U50,488 on 45Ca2- transport into R1.1 mouse thymoma cells is presented. This thymoma cell line expresses selectively the kappa-opioid class of receptors. 45Ca2+ transport into R1.1 cells was not affected by the kappa-opioid agonist (-)U50,488 (10(-10) M-10(-4) M) alone, or in the presence of the plant lectins: PHA (250 microg/ml) and Con A (800 microg/ml), after a 60 min treatment. The plant lectins PHA and Con A stimulated 45Ca2+ transport into R1.1 cells, in high concentrations (100-800 microg/ml) and (200-1000 microg/ml) respectively, after a 60 min treatment. Thus, 45Ca2+ transport was not affected in R1.1 cells by the kappa-opioid agonist (-)U50,488 alone, or in the presence of mitogens after a 60 min treatment. This negative result does not indicate the lack of calcium channels on R1.1 cells, since the plant lectins PHA and Con A were able to stimulate 45Ca2+ transport into R1.1 cells.
authors.affiliation: Ruder Bosković Institute, Division of Molecular Medicine, Zagreb, Croatia.
authors.forename: I
authors.initials: I
authors.lastname: Martin-Kleiner
authors.forename: J M
authors.initials: JM
authors.lastname: Bidlack
mesh_terms: D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology; D017473:Receptors, Opioid, kappa / Q000819:agonists*; D013945:Thymoma / Q000378:metabolism*; D013953:Thymus Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D010835:Phytohemagglutinins; D017473:Receptors, Opioid, kappa; D003208:Concanavalin A; D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; D002118:Calcium