Modulation of alpha-tropomyosin expression by alpha-tocopherol in rat vascular smooth muscle cells.
The effect of alpha-tocopherol (vitamin E) on gene expression in rat vascular smooth muscle cells was studied by the differential display technique. One gene out of about 1000 genes analyzed, identified as alpha-tropomyosin, showed an increased transcription level caused by alpha-tocopherol treatment. Northern and Western blot analysis revealed a time-dependent transient up-regulation of the amount of mRNA (peak between 2 and 3 h) and protein (peak at 5 h) in alpha-tocopherol-treated cells. No effect was observed in cells treated with beta-tocopherol.
Aratri
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E
Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
Spycher
S E
SE
Breyer
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I
Azzi
A
A
D000818:Animals; D001769:Blood; D003470:Culture Media; D005786:Gene Expression Regulation; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014335:Tropomyosin / Q000096:biosynthesis* / Q000235:genetics*; D014810:Vitamin E / Q000494:pharmacology*
D003470:Culture Media; D012333:RNA, Messenger; D014335:Tropomyosin; D014810:Vitamin E