title: Ultraviolet-B induction of interstitial collagenase and stromelyin-1 occurs in human dermal fibroblasts via an autocrine interleukin-6-dependent loop.
abstract: Ultraviolet-B irradiation of human dermal fibroblasts has earlier been shown to induce matrix-degrading metalloproteinases, thus driving connective tissue degradation in photoaging and photocarcinogenesis. Herein, we report that Ultraviolet-B irradiation led to a dramatic increase in specific mRNA and protein levels of interstitial collagenase, stromelysin and interleukin-6. By contrast, the major tissue inhibitor of matrix-degrading metalloproteinases, TIMP-1, was unaffected. Monospecific neutralizing antibodies directed against human interleukin-6 significantly reduced the interstitial collagenase and stromelysin-1 protein levels. Taken together, our data provide the first evidence that Ultraviolet-B induction of interstitial collagenase and stromelysin-1 occurs via the synthesis and release of interleukin-6. Hence, this newly identified autocrine mechanism may contribute to dermal photodamage.
authors.affiliation: Department of Dermatology, University of Cologne, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Brenneisen
authors.forename: M
authors.initials: M
authors.lastname: Wlaschek
authors.forename: J
authors.initials: J
authors.lastname: Wenk
authors.forename: R
authors.initials: R
authors.lastname: Blaudschun
authors.forename: R
authors.initials: R
authors.lastname: Hinrichs
authors.forename: J
authors.initials: J
authors.lastname: Dissemond
authors.forename: T
authors.initials: T
authors.lastname: Krieg
authors.forename: K
authors.initials: K
authors.lastname: Scharffetter-Kochanek
mesh_terms: D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D017364:Collagenases / Q000378:metabolism*; D004790:Enzyme Induction; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D020781:Matrix Metalloproteinase 1; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D009500:Neutralization Tests; D014466:Ultraviolet Rays
chemical_list: D015850:Interleukin-6; D017364:Collagenases; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1