Immunohistopathologic diagnosis of epidermolysis bullosa.
Routine histologic study usually is insufficient to subclassify epidermolysis bullosa (EB); currently, electron microscopic evaluation has been the gold standard. A major advance recently has been made in elucidating the molecular basis of several major forms of EB. Concomitantly, immunoreagents have been developed to map antigens in the basement membrane zone. Some of these reagents facilitate the classification of EB into types and subtypes and can be used as an adjunct informative screening procedure to direct mutation identification efforts using DNA technologies. The current review provides an overview of these recent developments and a more detailed account of the immunohistopathologic diagnosis of EB.
Bergman
R
R
Department of Dermatology, Rambam Medical Center, Haifa, Israel.
D001324:Autoantigens / Q000032:analysis; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D003937:Diagnosis, Differential; D000071821:Dystonin; D004820:Epidermolysis Bullosa / Q000378:metabolism / Q000473:pathology*; D016110:Epidermolysis Bullosa Simplex / Q000378:metabolism / Q000473:pathology; D016109:Epidermolysis Bullosa, Junctional / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D051190:Plectin; D012867:Skin / Q000737:chemistry / Q000473:pathology; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D051190:Plectin; D000097805:Collagen Type XVII; C486268:DST protein, human; C495332:PLEC protein, human