title: Co-existence of clpB and clpC in the Bacillaceae.
abstract: The gene encoding ClpC in Bacillus anthracis was amplified from the chromosome by polymerase chain reaction using degenerate oligonucleotide primers. These primers also amplified a second DNA fragment identified as a clpB homolog. Both genes were suggested to be functional. Contrary to Bacillus subtilis which possesses clpC but not clpB, many Bacillus species were found to harbor both clpC and clpB. We also found that Clostridium strains could possess clpB, clpC, or both. All the Gram-negative strains tested had clpB only.
authors.affiliation: Toxines et Pathogénie Bactériennes (CNRS URA 1858), Institut Pasteur, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Namy
authors.forename: M
authors.initials: M
authors.lastname: Mock
authors.forename: A
authors.initials: A
authors.lastname: Fouet
mesh_terms: D001406:Bacillaceae / Q000235:genetics* / Q000378:metabolism; D001408:Bacillus anthracis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D049071:Endopeptidase Clp; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C087481:ClpC protein, Bacteria; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D049071:Endopeptidase Clp; C069775:ClpB protein, E coli