Preliminary characterization of a reovirus isolated from golden ide Leuciscus idus melanotus.
Some characteristics of a reovirus recently isolated from golden ide Leuciscus idus melanotus and tentatively designated as golden ide reovirus (GIRV) were determined. Spherical non-enveloped particles with an outer capsid of about 70 nm and an inner capsid of about 50 nm were observed by electron microscopy. The density of the virus determined in CsCl gradients was 1.36 g ml-1. The genome contained 11 segments of dsRNA. GIRV differed from other aquareoviruses by a slight reduction of infectivity after treatment with chloroform and by the absence of forming syncytia in cell monolayers.
Neukirch
M
M
Fish Diseases Research Unit, School of Veterinary Medicine, Hannover, Germany. mneuki@fisch.tiho-hannover.de
Haas
L
L
Lehmann
H
H
von Messeling
V
V
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000662:veterinary; D002725:Chloroform / Q000494:pharmacology; D003530:Cyprinidae; D005393:Fish Diseases / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D018061:Infectious pancreatic necrosis virus / Q000276:immunology; D008854:Microscopy, Electron / Q000662:veterinary; D012087:Reoviridae / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D012088:Reoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000473:pathology / Q000662:veterinary / Q000821:virology
D007106:Immune Sera; D002725:Chloroform