title: Influence of iron on growth and extracellular products of Acinetobacter baumannii.
abstract: Iron is an important nutrient required by bacteria for optimal growth. Acquisition of iron from the host where iron is restricted is an important mediator of bacterial pathogenesis. In iron deplete chemically defined medium (CDM-Fe) growth of Acinetobacter baumannii was restricted as compared to iron replete medium (CDM + Fe). Bacteria developed four high molecular weight outer membrane proteins (OMPs) of 88, 84, 80 and 77 kDa in CDM-Fe medium which were absent in CDM + Fe medium, and are known iron regulated outer membrane proteins (IROMPs). A. baumannii secreted siderophores extracellularly into the medium which act as iron chelators which had been demonstrated in the supernatants of CDM-Fe media. The siderophore was of catechol type. This shows that A. baumannii under iron restricted conditions express IROMPs along with production of catechol type siderophore in order to acquire iron from the external milieu.
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Goel
authors.forename: A
authors.initials: A
authors.lastname: Kapil
authors.forename: B
authors.initials: B
authors.lastname: Das
authors.forename: D N
authors.initials: DN
authors.lastname: Rao
mesh_terms: D000150:Acinetobacter / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D000151:Acinetobacter Infections / Q000209:etiology / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D001693:Biological Transport, Active; D003470:Culture Media; D006801:Humans; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D008970:Molecular Weight; D017262:Siderophores / Q000096:biosynthesis; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D017262:Siderophores; D007501:Iron