Characterization of antigenic proteins from Tritrichomonas foetus recognized by antibodies in rabbit serum, bovine serum and bovine cervicovaginal mucus.
Tritrichomonas foetus is an obligate parasite of the bovine urogenital tract and is recognized as 1 of the more common infectious agents causing decreased reproductive efficiency in beef cattle. Infections result in reproductive failure and produce considerable economic loss. Vaccination of heifers with vaccines containing T. foetus induces elevated serological responses to many T. foetus antigens, decreases the rate and/or length of infection with T. foetus, and decreases fetal loss caused by infection. Because T. foetus infections are usually limited to lumen and mucosal surfaces of the reproductive tract, it has been assumed that protection from infection and abortion is partially mediated by immunoglobulins in the uterus and vagina. The objective of this study was to identify and characterize specific antigens of T. foetus that show promise for use in a recombinant vaccine that will generate a protective mucosal immune response in cattle. Surface proteins were identified by using polyclonal rabbit anti-trichomonal sera eluted from paraformaldehyde-fixed cells. Analyses of these proteins, utilizing mucosal antibodies from vaccinated and convalescent cows, have identified proteins involved in generating a local immune response. Western immunoblot analysis indicates that these proteins are well conserved and are excellent candidates for incorporation into a recombinant vaccine.
Gault
R A
RA
School of Veterinary Medicine, College of Agriculture, University of Nevada, Reno 89557-0046, USA.
Hall
M R
MR
Kvasnicka
W G
WG
Hanks
D R
DR
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology; D002582:Cervix Mucus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007526:Isoelectric Point; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011528:Protozoan Infections / Q000276:immunology / Q000469:parasitology; D011529:Protozoan Infections, Animal; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D016835:Tritrichomonas foetus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D014621:Vagina / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D037102:Lectins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D010504:Periodic Acid; C009288:metaperiodate