Lectin typing of methicillin-resistant Staphylococcus aureus.
This study reports the patterns of agglutination of 77 clinical isolates of methicillin-resistant Staphylococcus aureus by 32 commercially available lectins. Cell suspensions were not pre-treated. Each isolate was cultured on three media: Columbia blood agar, trypticase-soy agar and Chapman Stone agar. The lectins agglutinating each isolate varied widely depending on culture medium; only five isolates were agglutinated by the same set of lectins regardless of the culture medium used. Lectin typing could be a useful epidemiological tool, but it is necessary to standardise assay conditions (notably culture medium) to enable meaningful comparison of the results produced by different research groups or centres.
MUñTOZ
A
A
Department of Microbiology and Parasitology, Faculty of Biology, University of Santiago de Compostela.
Alvarez
O
O
Department of Microbiology and Parasitology, Faculty of Biology, University of Santiago de Compostela.
Alonso
B
B
Department of Microbiology and Parasitology, Faculty of Biology, University of Santiago de Compostela.
Llovo
J
J
Laboratory of Microbiology, Complex Hospitalario Universitano de Santiago (Hospital de Conxo), Spain.
D000372:Agglutination Tests / Q000379:methods*; D015373:Bacterial Typing Techniques; D037102:Lectins / Q000378:metabolism*; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects
D037102:Lectins