A new protocol to prepare dry plant specimens for electron microscopy and immunocytochemistry.
Samples from dry kidney bean cotyledons were fixed in acrolein vapor and embedded in Lowicryl K4M using a modified dehydration and embedding technique. The preservation of morphology as investigated by transmission electron microscopy was excellent showing desiccated cells and organelles in life-like preservation. Postembedding immunogold labeling for kidney bean purple acid phosphatase (KbPAP) showed association of kbPAP with ribosome-rich areas of the cytoplasm.
Grote
M
M
Institute of Medical Physics and Biophysics, University of Münster, Germany.
Reichelt
R
R
Wiermann
R
R
D000135:Acid Phosphatase / Q000032:analysis; D000171:Acrolein; D006023:Glycoproteins / Q000032:analysis; D007150:Immunohistochemistry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D012116:Resins, Plant; D012639:Seeds / Q000737:chemistry / Q000648:ultrastructure*; D016707:Tissue Fixation / Q000379:methods*
D006023:Glycoproteins; D012116:Resins, Plant; D000171:Acrolein; C091137:purple acid phosphatase; D000135:Acid Phosphatase