Zinc is required in pyrrolidine dithiocarbamate inhibition of NF-kappaB activation.
Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF-kappaB) activation. PDTC inhibited basal NF-kappaB activity of endothelial cells. PDTC, however, failed to inhibit basal NF-kappaB activity after withdrawal of serum in the media, and the inhibitory effect of PDTC could be restored by addition of zinc. When various preparations of metal ion-EDTA were tested with PDTC in serum-containing media, only Zn-EDTA failed to block the inhibitory effect of PDTC. The dependence on zinc was also noted in PDTC inhibition of NF-kappaB stimulated by TNF alpha. These facts suggest that zinc is required for PDTC inhibition of NF-kappaB activation.
Kim
C H
CH
Yonsei Brain Research Institute and Department of Pharmacology, Yonsei University College of Medicine, Seoul, South Korea.
Kim
J H
JH
Hsu
C Y
CY
Ahn
Y S
YS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D002412:Cations; D002417:Cattle; D002478:Cells, Cultured; D008670:Metals; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D011759:Pyrrolidines / Q000378:metabolism* / Q000494:pharmacology; D013859:Thiocarbamates / Q000378:metabolism* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism*
D000975:Antioxidants; D002412:Cations; D008670:Metals; D016328:NF-kappa B; D011759:Pyrrolidines; D013859:Thiocarbamates; D014409:Tumor Necrosis Factor-alpha; C020972:pyrrolidine dithiocarbamic acid; D015032:Zinc