Evidence of oxygen free radical damage in human otitis media.
Recent work with a guinea pig model of otitis media has demonstrated evidence of oxygen free radical damage to the middle ear mucosa. However, the relevance of an animal model to human disease is uncertain. Accordingly, the following pilot study was conducted to examine human middle ear fluid for lipid hydroperoxides as evidence of free radical damage. Thirty-five specimens of middle ear fluid from children with chronic otitis media were collected and described as mucoid (n = 19), purulent (n = 10), or serous (n = 6); specimens were weighed and analyzed for lipid hydroperoxide content. The results demonstrated the presence of lipid hydroperoxide in all 3 types of middle ear fluid. Additionally, there was a statistically significant elevation of total lipid hydroperoxide content in mucoid effusions compared with serous effusions, as well as a significant elevation of lipid hydroperoxide divided by weight of purulent effusions compared with serous effusions. This is the first study to document free radical damage in human otitis media.
Takoudes
T G
TG
Division of Pediatric Otolaryngology-Head and Neck Surgery, Babies & Children's Hospital of New York, Columbia-Presbyterian Medical Center, New York 10032, USA.
Haddad
J
J
D001826:Body Fluids / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D005260:Female; D005609:Free Radicals / Q000009:adverse effects / Q000276:immunology; D006801:Humans; D007223:Infant; D008054:Lipid Peroxides / Q000032:analysis*; D008297:Male; D008876:Middle Ear Ventilation; D010033:Otitis Media / Q000145:classification / Q000276:immunology* / Q000473:pathology* / Q000601:surgery; D017382:Reactive Oxygen Species / Q000276:immunology*
D005609:Free Radicals; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species