Detection of surface antigen 17-1A in breast and colorectal cancer.
Substantial progress has been made in detecting cell surface or intracytoplasmatic antigens to identify spread tumor cells with monoclonal antibodies (MAbs). The 17-1A antigen is already used as a target for specific immunotherapy in colorectal cancer. The purpose of this study was to compare the expression of 17-1A antigen in colorectal tumors versus breast cancers. MAb against the epithelial-specific antigen (ESA) and a routine staining technique were used to detect the 17-1A antigen in 100 cases of colorectal and 111 cases of breast cancer. The antigen expression of each tumor entity was examined by light microscopy on paraffin sections. Thirty six of the formalin-fixed paraffin sections of breast cancer were compared with their corresponding frozen sections. Evaluation was realized by a histological score (grade 0-9) considering the distribution and the staining intensity. We found an antigen expression of 17-1A in colorectal cancer quantified at 7.1+/-1.8 and at 4.5+/-2.5 for breast cancer in our score. Comparing paraffin sections and frozen sections in the 36 cases of breast cancer, the score was 5.5+/-2.3 in the paraffin and 8.1+/-1.9 in the frozen section group. Our results confirmed the high expression of 17-1A cases of in colorectal carcinoma. Furthermore, 17-1A is expressed in the majority of breast carcinomas, revealing a high difference between paraffin and frozen sections. As a result, a specific immunotherapy with MAbs against 17-1A antigen in minimal residual stages of breast cancer might be considered.
Packeisen
J
J
Institute of Pathology, Bethesda Hospital, Duisburg, Germany.
Kaup-Franzen
C
C
Knieriem
H J
HJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D001943:Breast Neoplasms / Q000737:chemistry* / Q000276:immunology; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000276:immunology; D005260:Female; D005557:Formaldehyde; D005615:Freezing; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C050928:carcinoma-associated antigen 17-1A; D005557:Formaldehyde