title: A new solid-phase synthesis of oligonucleotides 3'-conjugated with peptides.
abstract: A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the first nucleoside via the base has been developed. A 17-mer designed for antisense experiments against HIV-1, linking at the 3'-terminus the tripeptide Gly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università degli Studi di Napoli Federico II, Italy.
authors.forename: L
authors.initials: L
authors.lastname: De Napoli
authors.forename: A
authors.initials: A
authors.lastname: Messere
authors.forename: D
authors.initials: D
authors.lastname: Montesarchio
authors.forename: G
authors.initials: G
authors.lastname: Piccialli
authors.forename: E
authors.initials: E
authors.lastname: Benedetti
authors.forename: E
authors.initials: E
authors.lastname: Bucci
authors.forename: F
authors.initials: F
authors.lastname: Rossi
mesh_terms: D002621:Chemistry / Q000379:methods*; D015497:HIV-1 / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010452:Peptide Biosynthesis; D013696:Temperature
chemical_list: D009841:Oligonucleotides