The role of roots in cysteine biosynthesis by Arabidopsis thaliana.
O-Acetylserine(thiol)lyase (OASTL) enzyme catalyzes the cysteine biosynthesis in photosynthetic organisms. In mature Arabidopsis thaliana the highest activity level is observed in non-photosynthetic tissues like roots, that also show significant amount of protein detected by Western blot analysis. By means of specific probes for cytosolic and plastidic OASTL isoenzyme transcripts, cytosolic isoenzyme has been determined to be predominantly expressed in roots, while the expression of the plastidic isoform is high in both green and non-green tissues. Sulfur starvation produces an increase on the OASTL activity level in all the Arabidopsis organs examined, this effect being particularly significant in the aerial parts of the plant.
Barroso
C
C
Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, Spain.
Vega
J M
JM
Gotor
C
C
D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism*; D003545:Cysteine / Q000096:biosynthesis*; D003547:Cysteine Synthase / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000502:physiology*; D013455:Sulfur / Q000378:metabolism
D013455:Sulfur; D003547:Cysteine Synthase; D003545:Cysteine