A second gene encoding the mouse proteasome activator PA28beta subunit is part of a LINE1 element and is driven by a LINE1 promoter.
The PMSE2 gene encodes the beta-subunit of the proteasome activator PA28 and, as shown by genomic Southern blot analysis, there probably exist four copies sharing sequence homology with PMSE2. Here, we report that in the mouse genome there exist two different chromosomal loci for PA28beta, both of which are transcribed and and which encode a functional PA28beta subunit. One of these represents the previously described PMSE2 gene possessing an intron-exon structure and a gamma interferon (IFNgamma)-inducible promoter. The second one, named PMSE2b, which we describe here, exhibits all the characteristics of an expressed retrotransposon. Our data show that the PA28beta retrotransposon is inserted into a transcriptional active LINE1 element and is driven by a LINE1 F-type monomer promoter as revealed by luciferase assays. The resulting PMSE2b mRNA encodes a protein which is indistinguishable from that encoded by the IFNgamma-inducible PMSE2 gene. Since PA28 plays an important role in major histocompatibility complex class I antigen presentation, the implications for the mouse immune system through a constitutively expressed PA28beta subunit and the biological relevance of this finding are discussed.
Zaiss
D M
DM
Institute of Biochemistry-Charité, Humboldt University Medical School, Humboldt University, Monbijoustrasse 2, Berlin, 10117, Germany.
Kloetzel
P M
PM
D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D020084:Long Interspersed Nucleotide Elements; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D046988:Proteasome Endopeptidase Complex; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D018626:Retroelements; D014158:Transcription, Genetic
D001324:Autoantigens; C099558:Ki antigen; D011506:Proteins; D018626:Retroelements; D046988:Proteasome Endopeptidase Complex; C486214:Psme2 protein, mouse