title: 4-hydroxycinnamoyl-CoA hydratase/lyase (HCHL)--An enzyme of phenylpropanoid chain cleavage from Pseudomonas.
abstract: The enzyme 4-hydroxycinnamoyl-CoA hydratase/lyase (HCHL), which catalyzes a hydration and two-carbon cleavage step in the degradation of 4-hydroxycinnamic acids, has been purified and characterized from Pseudomonas fluorescens strain AN103. The enzyme is a homodimer and is active with three closely related substrates, 4-coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA (Km values: 5.2, 1.6, and 2.4 microM, respectively), but not with cinnamoyl-CoA or with sinapinoyl-CoA. The abundance of the enzyme reflects a low catalytic center activity (2.3 molecules s-1 at 30 degrees C; 4-coumaroyl-CoA as substrate).
authors.affiliation: Institute of Food Research-Norwich Laboratory, Norwich Research Park, Norwich, Colney, NR4 7UA, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Mitra
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: M J
authors.initials: MJ
authors.lastname: Gasson
authors.forename: A
authors.initials: A
authors.lastname: Narbad
authors.forename: A J
authors.initials: AJ
authors.lastname: Parr
authors.forename: J
authors.initials: J
authors.lastname: Payne
authors.forename: M J
authors.initials: MJ
authors.lastname: Rhodes
authors.forename: C
authors.initials: C
authors.lastname: Sewter
authors.forename: N J
authors.initials: NJ
authors.lastname: Walton
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D019281:Dimerization; D004795:Enzyme Stability; D006836:Hydro-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D002934:Cinnamates; D003373:Coumaric Acids; C119167:feruloyl-CoA; C058644:4-coumaroyl-coenzyme A; C058645:caffeoyl-coenzyme A; C022903:cinnamoyl-coenzyme A; C004999:ferulic acid; C119166:4-hydroxycinnamoyl-CoA hydratase-lyase; D006836:Hydro-Lyases