Pseudoazurin mediates periplasmic electron flow in a mutant strain of Paracoccus denitrificans lacking cytochrome c550.
A periplasmic protein able to transfer electrons from cytoplasmic membrane to the periplasmic nitrite reductase (cytochrome cd1) has been purified from the anoxically grown cytochrome c550 mutant strain Pd2121 and shown to be pseudoazurin by several independent criteria (molecular mass, copper content, visible spectrum, N-terminal amino acid sequence). Under our assay conditions, the half-saturation of electron transport occurred at about 10 microM pseudoazurin; the reaction was retarded by increasing ionic strength.
Koutný
M
M
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Kucera
I
I
Tesarík
R
R
Turánek
J
J
Van Spanning
R J
RJ
D000595:Amino Acid Sequence; D001400:Azurin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D003574:Cytochrome c Group / Q000502:physiology*; D004579:Electron Transport; D008969:Molecular Sequence Data; D009154:Mutation; D010231:Paracoccus denitrificans / Q000235:genetics / Q000378:metabolism*; D019897:Periplasm / Q000378:metabolism; D011549:Pseudomonas
D003574:Cytochrome c Group; C047163:pseudoazurin; D001400:Azurin; D003300:Copper; C031732:cytochrome C-550