title: PsiI, a novel restriction endonuclease recognizing the DNA sequence 5'-TTA downward arrow TAA-3'.
abstract: PsiI, a novel restriction endonuclease produced by the bacterial strain Pseudomonas sp. SE-G49, has been isolated and characterized. The enzyme cleaves DNA in the middle of its palindromic recognition sequence 5'-TTA downward arrow TAA-3'. Thus, PsiI belongs to a rare group of type II restriction endonucleases whose recognition sites consist of AT base pairs only.
authors.affiliation: SibEnzyme Co., Novosibirsk, 630117, Russia. se_info@ma.nsc.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Abdurashitov
authors.forename: O A
authors.initials: OA
authors.lastname: Belichenko
authors.forename: N A
authors.initials: NA
authors.lastname: Lebedeva
authors.forename: S K
authors.initials: SK
authors.lastname: Degtyarev
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006868:Hydrolysis; D013379:Substrate Specificity
chemical_list: D004247:DNA; C120006:endodeoxyribonuclease PsiI; D015252:Deoxyribonucleases, Type II Site-Specific