title: Splanchnic tissue perfusion in acute experimental pancreatitis.
abstract: BACKGROUND
  Gut hypoperfusion may have a role in the pathogenesis of multiple organ failure, which is a the main cause of death in severe acute pancreatitis. We hypothesized that gut hypoperfusion is present early in acute pancreatitis and that supporting the systemic hemodynamics by fluid resuscitation would prevent this.
  
  
  METHODS
  In a pig model of randomized, controlled experimental hemorrhagic pancreatitis induced by Na-taurocholate the animals were divided into four groups (n = 6 for each): 1) pancreatitis, 2) control, 3) pancreatitis and fluid resuscitation to keep the pulmonary capillary wedge pressure at 5 to 6 mmHg, and 4) control and fluid resuscitation as in group 3. Splanchnic perfusion was assessed by means of local PCO2 gap with intestinal tonometer, oxygen delivery and consumption, lactate production, and blood flow. The follow-up time was 6 h.
  
  
  RESULTS
  The Pco2 gap increased in pancreatitis (1.72+/-0.17, 1.94+/-0.29, 1.75+/-0.22, 2.32+/-0.33; 9.40+/-2.16, 3.72+/-1.78, 0.84+/-0.39, 1.11+/-0.21 kPa, respectively; P < 0.05). Oxygen delivery in portal-drained organs decreased in pancreatitis (2.5+/-0.3, 2.6+/-0.2, 2.8+/-0.4, 2.3+/-0.2; 1.7+/-0.3, 2.3+/-0.3, 2.4+/-0.5, 2.3+/-0.3 ml/min x kg, respectively; P < 0.05). Regional oxygen consumption did not change. Arterial plasma lactate increased (1.20+/-0.19, 1.33+/-0.16, 1.14+/-0.15, 1.43+/-0.33; 3.81+/-1.31, 1.48+/-0.48, 1.12+/-0.18, 1.18+/-0.35 mmol/l, respectively; P < 0.05). The portal venous blood flow decreased 50% in pancreatitis, but with fluid resuscitation it increased 50%.
  
  
  CONCLUSIONS
  Splanchnic hypoperfusion is present early in acute hemorrhagic pancreatitis. The signs of hypoperfusion can be prevented with fluid resuscitation.
authors.affiliation: Dept. of Surgery, Kuopio University Hospital, Finland.
authors.forename: P O
authors.initials: PO
authors.lastname: Juvonen
authors.forename: J J
authors.initials: JJ
authors.lastname: Tenhunen
authors.forename: A A
authors.initials: AA
authors.lastname: Heino
authors.forename: M
authors.initials: M
authors.lastname: Merasto
authors.forename: H E
authors.initials: HE
authors.lastname: Paajanen
authors.forename: E M
authors.initials: EM
authors.lastname: Alhava
authors.forename: J A
authors.initials: JA
authors.lastname: Takala
mesh_terms: D000818:Animals; D005440:Fluid Therapy; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000503:physiopathology*; D007422:Intestines / Q000098:blood supply*; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000503:physiopathology*; D011897:Random Allocation; D013152:Splanchnic Circulation / Q000502:physiology*; D013552:Swine; D013656:Taurocholic Acid
chemical_list: D013656:Taurocholic Acid