title: The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
abstract: The mAb 2A10 recognizes a 120-kDa protein with sequence homology to the human CD163 and whose expression is restricted to the cells of the porcine monocyte/macrophage lineage. While most of tissue macrophages express high levels of 2A10 Ag, bone marrow cells and a subset of blood monocytes are negative for this marker. The percentage of 2A10+ blood monocytes ranges between 5-50% depending on the donor. The phenotypic analysis indicates that these cells are more similar to mature macrophages than 2A10- monocytes. 2A10+ monocytes express higher levels of swine histocompatibility leukocyte Ag II, CD16, and the adhesion molecules very late Ag-4 (CD49d) and LFA-1 (CD11a) than 2A10- monocytes, while CD14 and SWC1 expression is lower. Both monocyte subsets also differ in their functional capabilities. 2A10+ monocytes induce a greater allogeneic response on T lymphocytes than 2A10- cells. LPS-stimulated 2A10+ and 2A10- monocytes both produce proinflammatory cytokines (TNF-alpha and IL-1alpha), but antiinflammatory IL-10 is only detected on the latter population. When 2A10- monocytes were cultured in medium containing pig serum, they acquired some phenotypic features of 2A10+ cells, expressing the 2A10 Ag. In contrast, when they were cultured in the presence of L929 supernatant as a source of GM-CSF, the 2A10 Ag expression remained low, scarcely increasing over basal levels. 2A10+ cells cultured with pig serum developed features that resemble monocyte-derived dendritic cells. These results indicate that 2A10+ monocytes could constitute a cell population in a more advanced maturation stage than 2A10- circulating monocytes.
authors.affiliation: Departamento Sanidad Animal, Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: N
authors.initials: N
authors.lastname: Doménech
authors.forename: J
authors.initials: J
authors.lastname: Vázquez
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D016130:Immunophenotyping; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D009000:Monocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism; D013552:Swine / Q000276:immunology*; D000098902:CD163 Antigen
chemical_list: C118892:2A10 antigen, porcine; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D016207:Cytokines; D010446:Peptide Fragments; D011956:Receptors, Cell Surface