Human nuclear matrix contains a protein complex which can specifically recognize alpha-satellite DNA in vitro.
A nuclear matrix protein complex has been found which specifically binds to alpha-satellite DNA as revealed by gel shift assay. The complex contains three proteins--two DNA-binding proteins with molecular masses of 70 and 80 kD and a 58-kD protein which does not bind DNA but enhances the specificity of binding of the others to alpha-satellite DNA. The complex does not contain CENP-A, CENP-B, CENP-C, CENP-G, or lamins A/C and B.
Enukashvily
N I
NI
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia. natella@ivm.stud.pu.ru.
Lobov
I B
IB
Podgornaya
O I
OI
D034961:Antigens, Nuclear; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004276:DNA, Satellite / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding
D034961:Antigens, Nuclear; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins