Apolipoprotein E polymorphism in sheep.
Using polyacrylamide gel isoelectric focusing followed by immunoblotting with anti-human apolipoprotein E (APO E) antibody, the genetic polymorphism of APO E was determined from desialylated plasma of 554 unrelated adults of four European sheep (Suffolk, Corriedale, Cheviot, and Finnish Landrace) and five Asian local sheep (Bhyanglung, Baruwal, Kagi, Lampuchhre, and Vietnamese). Twenty phenotypes consisting of the homozygous and heterozygous combinations of two APO E variants within the seven variants (E1-E7) detected were identified. Family and population data supported the hypothesis that the phenotypes are controlled by seven codominant alleles, designated APOE1 to APOE7, at a single autosomal locus. The common alleles, APOE4, APOE5, and APOE7 were observed at mean frequencies of 0.5763, 0.1471, and 0.1921 in the European sheep group and 0.4920, 0.1123, and 0.2995 in the Asian local sheep group, respectively.
Tsunoda
K
K
Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.
Yamamoto
Y
Y
Akiya
Y
Y
Sato
K
K
Rajbhandary
H B
HB
van Son
H
H
Loc
C B
CB
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006579:Heterozygote; D006720:Homozygote; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity
D001057:Apolipoproteins E