title: Leishmaniosis and generalized demodicosis in three dogs: a clinicopathological and immunohistochemical study.
abstract: This paper describes the clinicopathological and immunohistochemical aspects of the skin lesions in three dogs with leishmaniosis and generalized demodicosis. Diffuse alopecia, crusts, folliculitis and furunculosis, as commonly seen in generalized demodicosis, were prominent in all the dogs. MicroIscopically, there was a diffuse and perifollicular superficial and deep granulomatous dermatitis and, in two dogs, both Copyright Demodex canis mites and Leishmania spp. amastigotes were observed in the same lesions. Numerous Mac387(+)macrophages were observed in the inflammatory infiltrates, but macrophages loaded with amastigotes were Mac387(-). In all cases, immunoreactive CD3 lymphocytes were sparse, both in the granulomatous and perifollicular infiltrates. There were numerous IgG+, IgG4(+)-secreting plasma cells in areas of folliculitis and furunculosis and fewer IgG2(+), IgG3(+), IgA+and IgM+-secreting plasma cells in the inflammatory infiltrate. In all cases, MHC Class II was expressed by the majority of dermal macrophages and dendritic cells, as well as by lymphocytes and fibroblasts. The paucity of CD3(+)lymphocytes, usually abundant in D. canis lesions, points to leishmania-induced cell-mediated immunosuppression as a predisposing factor for generalized demodicosis.
authors.affiliation: Facultad de Veterinaria, Universidad de Córdoba, Avenida Medina Azahara 9, Córdoba, 14005, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Mozos
authors.forename: J
authors.initials: J
authors.lastname: Pérez
authors.forename: M J
authors.initials: MJ
authors.lastname: Day
authors.forename: R
authors.initials: R
authors.lastname: Lucena
authors.forename: P J
authors.initials: PJ
authors.lastname: Ginel
mesh_terms: D000505:Alopecia / Q000150:complications / Q000473:pathology / Q000662:veterinary; D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry; D007896:Leishmaniasis / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D008297:Male; D008924:Mite Infestations / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D011711:Pyoderma / Q000150:complications / Q000473:pathology / Q000662:veterinary; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D017252:CD3 Complex