Secretion of lecithin:cholesterol acyltransferase by brain neuroglial cell lines.
The ability of different human and rat brain cell lines (neuronal and gliomal) to secrete lecithin:cholesterol acyltransferase (LCAT) was examined. Of these, the strongly secreting human gliomal (U343 and U251) cell lines were selected for a detailed study of enzymatic and structural properties of the secreted LCAT. Both plasma- and brain-derived enzymes are inhibited by DTNB (90%) and are activated by apolipoprotein A-I. LCAT mRNA was measured in these cell lines at levels similar to that found in HepG2 cells. In contrast, apoA-I, apoE, and apoD mRNAs were undetectable in these cell lines. The presence of functional LCAT secreted by cultured nerve cells provides an in vitro model to study the expression and function of LCAT in the absence of others factors of plasma cholesterol metabolism.
Collet
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Phospholipides membranaires, Signalisation cellulaire et Lipoprotéines, Hopital Purpan, INSERM U326, Institut National de la santé et de la Recherche Médicale, Unité 326, Toulouse, 31059, France. collet@cict.fr
Francone
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Besnard
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Fielding
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CJ
D000818:Animals; D001053:Apolipoproteins / Q000235:genetics; D001921:Brain / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D051379:Mice; D009457:Neuroglia / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
D001053:Apolipoproteins; D012333:RNA, Messenger; D007862:Phosphatidylcholine-Sterol O-Acyltransferase