title: alpha-D-Glcp-(1<-->1)-beta-D-Galp-containing oligosaccharides, novel products from lactose by the action of beta-galactosidase.
abstract: A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)]n-alpha-D-Glcp- (1<-->1)-beta-D-Galp[-(4<--1)-beta-D-Galp]m, with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp- (1<-->1)-beta-D-Galp.
authors.affiliation: Department of Bio-Organic Chemistry, Utrecht University, Netherlands.
authors.forename: C T
authors.initials: CT
authors.lastname: Fransen
authors.forename: K M
authors.initials: KM
authors.lastname: Van Laere
authors.forename: A A
authors.initials: AA
authors.lastname: van Wijk
authors.forename: L P
authors.initials: LP
authors.lastname: Brüll
authors.forename: M
authors.initials: M
authors.lastname: Dignum
authors.forename: J E
authors.initials: JE
authors.lastname: Thomas-Oates
authors.forename: J
authors.initials: J
authors.lastname: Haverkamp
authors.forename: H A
authors.initials: HA
authors.lastname: Schols
authors.forename: A G
authors.initials: AG
authors.lastname: Voragen
authors.forename: J P
authors.initials: JP
authors.lastname: Kamerling
authors.forename: J F
authors.initials: JF
authors.lastname: Vliegenthart
mesh_terms: D002240:Carbohydrate Sequence; D007785:Lactose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: C119562:Glcp-(1-1)-Galp; D009844:Oligosaccharides; D001616:beta-Galactosidase; D007785:Lactose