Safe and efficient peripheral blood stem cell collection in the smallest of children.
To clarify the factors that may affect the peripheral blood stem cell (PBSC) collection in children weighing < or = 15 kg, a consecutive registry of 109 leukapheresis procedures was analyzed. Collections were performed on a COBE Spectra separator. In 65.1% of the procedures, the peripheral vein, together with a central catheter inserted routinely at diagnosis, or 2 peripheral veins were used to access/return. For 84.4% of the procedures, the extracorporeal line was primed with red blood cells. The median granulocyte-macrophage colony forming unit (CFU-GM) number derived from 1 patient's blood volume processed was 13.8 x 10(4)/kg. Six times, a collection series failed, always in children treated for > or = 26 weeks and 4 of those times in children weighing < or = 11 kg. The patient's age, diagnosis, duration of preleukapheresis treatment, and mobilization regimens did not significantly affect the collection yield. Twenty-four transplantations were performed. The median times to neutrophils >0.5 x 10(9)/L and platelets >20 x 10(9)/L were 13 and 20 days, respectively. We conclude that even in very small children, leukapheresis can be performed safely, allowing adequate PBSC collection for transplantation and/or in vitro manipulations.
Kanold
J
J
Unité Bioclinique de Prélèvement et de Traitement des Cellules Souches Hématopoïétiques, CHU Clermont-Ferrand, France.
Halle
P
P
Rapatel
C
C
Berger
M
M
Gembara
P
P
deLumley
L
L
Vannier
J P
JP
Lutz
P
P
Plantaz
D
D
Malpuech
G
G
Deméocq
F
F
D000367:Age Factors; D001810:Blood Volume; D001835:Body Weight; D002675:Child, Preschool; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D007958:Leukocyte Count; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000628:therapy*; D009504:Neutrophils; D010976:Platelet Count; D012189:Retrospective Studies; D016896:Treatment Outcome