Identification of squamous cell carcinoma antigen-2 in tumor tissue by two-dimensional electrophoresis.
The aim of this study was to identify two homologous serine proteinase inhibitor (serpin) molecules, squamous cell carcinoma (SCC) antigen-1 and -2, by two-dimensional electrophoresis (2-DE), combined with immunoblotting, and examine their expression in tumor tissue. The recombinant SCC (rSCC) antigen-1 showed four spots with p/ 6.5, 6.4, 6.3 and 6.0, whereas rSCC antigen-2 showed a more acidic spot with p/5.95. SCC antigen in tumor tissue appeared in three new acidic spots (p/5.7-5.5, M(r) 44 500), numbered 5, 6 and 7, besides the previously reported four spots numbered 1 to 4. These new acidic spots of SCC antigen apparently increased in SCC tissue. Treatment of tissue extract by carboxymethyl (CM)-papain agarose matrix extinguished spots 1 to 4 encoded on the SCCA1 gene, but not 5 to 7 on the SCCA2 gene. Overexpression of the SCCA2 gene may play an important role in the malignant behavior of tumor cells.
Nawata
S
S
Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Ube, Japan. obgyn@po.cc.yamaguchi-u.ac.jp
Murakami
A
A
Hirabayashi
K
K
Sakaguchi
Y
Y
Ogata
H
H
Suminami
Y
Y
Numa
F
F
Nakamura
K
K
Kato
H
H
D000951:Antigens, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D010206:Papain; D011994:Recombinant Proteins / Q000032:analysis; D012685:Sepharose; D015843:Serpins
D000951:Antigens, Neoplasm; D011994:Recombinant Proteins; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D012685:Sepharose; D010206:Papain