Elevated growth of Saccharomyces cerevisiae ATH1 null mutants on glucose is an artifact of nonmatching auxotrophies of mutant and reference strains.
Yeast strains disrupted for ATH1, which encodes vacuolar acid trehalase, have been reported to grow to higher cell densities than reference strains. We showed that the increase in cell density is due to the URA3 gene introduced as a part of the disruption and concluded that the misinterpretation is a result of not using a control strain with matching auxotrophic markers.
Chopra
R
R
Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
Sharma
V M
VM
Ganesan
K
K
D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D005819:Genetic Markers; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014198:Trehalase / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D005656:Fungal Proteins; D005819:Genetic Markers; D029701:Saccharomyces cerevisiae Proteins; C097090:ATH1 protein, S cerevisiae; D014198:Trehalase; D005947:Glucose