title: Co-expression of gene 31 and 23 products of bacteriophage T4.
abstract: Folding of the major capsid protein of bacteriophage T4 encoded by gene 23 is aided by Escherichia coli GroEL chaperonin and phage co-chaperonin gp31. In the absence of gene product (gp) 31, aggregates of recombinant gp23 accumulate in the cell similar to inclusion bodies. These aggregates can be solubilized with 6 M urea. However, the protein cannot form regular structures in solution. A system of co-expression of gp31 and gp23 under the control of phage T7 promoter in E. coli cells has been constructed. Folding of entire-length gp23 (534 amino acid residues) in this system results in the correctly folded recombinant gp23, which forms long regular structures (polyheads) in the cell.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117871, Russia. vvm@ibch.siobc.ras.ru.
authors.forename: L P
authors.initials: LP
authors.lastname: Kurochkina
authors.forename: V V
authors.initials: VV
authors.lastname: Mesyanzhinov
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D036022:Capsid Proteins; D003001:Cloning, Molecular; D017931:DNA Primers; D008854:Microscopy, Electron; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D014764:Viral Proteins; C063473:gene 31 protein, Enterobacteria phage T4; C064517:gp23 protein, Bacteriophage T4