Evidence for peroxynitrite formation during S-nitrosoglutathione photolysis in air saturated solutions.
Flash photolysis of S-nitrosoglutathione (GSNO) in aerated solutions at pH 10 gave rise to an absorption with a maximum around 310-320 nm. This peak is spectrally similar to that displayed by ONOO-. The decay kinetics of this absorption was compared to that of authentic ONOO-, generated independently. An excellent correlation was obtained. Further proof of ONOO- generation was provided by HPLC studies showing the production of 3-nitrotyrosine on irradiation of GSNO in the presence of tyrosine at pH 7.4. In addition, the nitration yield was increased approximately 5-fold in the presence of bicarbonate and totally eliminated with DMPO, indicating the requirement of a radical intermediate for peroxynitrite production during S-nitrosothiol photolysis.
Mutus
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Chemistry and Biochemistry, School of Physical Sciences, University of Windsor, Ont., Canada. mutusb@uwindsor.ca
Redmond
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Akhter
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D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism*; D010782:Photolysis; D026422:S-Nitrosoglutathione; D012996:Solutions
D009566:Nitrates; D009603:Nitroso Compounds; D012996:Solutions; C039642:peroxynitric acid; D026422:S-Nitrosoglutathione; D005978:Glutathione