The presence of a muscarinic receptor on canine erythrocyte membranes.
Muscarinic receptors were present on erythrocyte ghosts of the domestic dog (Canis familiaris) at a concentration of 0.61 nmol/mg protein, and had a KD of 62.5 nmol. Properties including a mean nonspecific binding of 80%, the high risk that adherence of additional molecules of radioligand would occur, and the necessity for extensive dilution of erythrocyte ghosts to obtain a practical quantity of muscarinic receptors, lead us to conclude that the canine erythrocyte ghost is not an efficient or practical model to study muscarinic receptors and their interactions with cholinergic agonists or antagonists.
Dalefield
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Comparative Toxicology Laboratories, Kansas State University, Manhattan 66506-5606, USA.
Oehme
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D000818:Animals; D004285:Dogs; D004910:Erythrocyte Membrane / Q000378:metabolism*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000494:pharmacology; D011976:Receptors, Muscarinic / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors
D018727:Muscarinic Antagonists; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate