Cloning of oxetanocin A biosynthetic and resistance genes that reside on a plasmid of Bacillus megaterium strain NK84-0128.
Bacillus megaterium strain NK84-0218 produces a potent antiviral antibiotic, oxetanocin A, which has an oxetanosyl-N-glycoside linkage to an adenine moiety. However, the oxetanocin A productivity of the original strain was unstable and low. In this study, oxetanocin A productivity and resistance was shown to be lost simultaneously when a 51.5-kb plasmid, pOXT1, was cured during cultivation. The deficiency of oxetanocin A productivity and resistance was restored by re-introduction of the pOXT1 plasmid into the cured strain. By a cloning experiment it was shown that a 6.8-kb BglI-D fragment of the pOXT1 plasmid was responsible for oxetanocin A productivity and resistance.
Morita
M
M
Research and Development Division, Nippon Kayaku Co. Ltd., Tokyo, Japan.
Tomita
K
K
Ishizawa
M
M
Takagi
K
K
Kawamura
F
F
Takahashi
H
H
Morino
T
T
D000225:Adenine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000998:Antiviral Agents / Q000378:metabolism*; D001410:Bacillus megaterium / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D009154:Mutation; D010957:Plasmids / Q000235:genetics*
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; C051061:oxetanocin; D000225:Adenine