title: Assisted conception in HIV discordant couples: evaluation of semen processing techniques in reducing HIV viral load.
abstract: Artificial insemination with motile spermatozoa prepared from HIV-infected men using standard procedures has been employed with many HIV-discordant couples. We have demonstrated that processing semen from HIV positive men can reduce HIV levels, measured as HIV1 RNA copies/ml using nucleic acid based sequence amplification (NASBA), to undetectable levels (less than 400 copies/ml) but not in all samples. We believe that all processed samples should be tested prior to insemination.
authors.affiliation: Department of Virology, Guy's Hospital Trust, London, UK.
authors.forename: I L
authors.initials: IL
authors.lastname: Chrystie
authors.forename: J E
authors.initials: JE
authors.lastname: Mullen
authors.forename: P R
authors.initials: PR
authors.lastname: Braude
authors.forename: P
authors.initials: P
authors.lastname: Rowell
authors.forename: E
authors.initials: E
authors.lastname: Williams
authors.forename: N
authors.initials: N
authors.lastname: Elkington
authors.forename: A
authors.initials: A
authors.lastname: de Ruiter
authors.forename: K
authors.initials: K
authors.lastname: Rice
authors.forename: J
authors.initials: J
authors.lastname: Kennedy
mesh_terms: D005069:Evaluation Studies as Topic; D005260:Female; D015658:HIV Infections / Q000517:prevention & control / Q000821:virology*; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D012661:Semen / Q000821:virology*; D019562:Viral Load