Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene.
Strain DCL14, which is able to grow on limonene as a sole source of carbon and energy, was isolated from a freshwater sediment sample. This organism was identified as a strain of Rhodococcus erythropolis by chemotaxonomic and genetic studies. R. erythropolis DCL14 also assimilated the terpenes limonene-1,2-epoxide, limonene-1,2-diol, carveol, carvone, and (-)-menthol, while perillyl alcohol was not utilized as a carbon and energy source. Induction tests with cells grown on limonene revealed that the oxygen consumption rates with limonene-1,2-epoxide, limonene-1,2-diol, 1-hydroxy-2-oxolimonene, and carveol were high. Limonene-induced cells of R. erythropolis DCL14 contained the following four novel enzymatic activities involved in the limonene degradation pathway of this microorganism: a flavin adenine dinucleotide- and NADH-dependent limonene 1, 2-monooxygenase activity, a cofactor-independent limonene-1, 2-epoxide hydrolase activity, a dichlorophenolindophenol-dependent limonene-1,2-diol dehydrogenase activity, and an NADPH-dependent 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity. Product accumulation studies showed that (1S,2S,4R)-limonene-1,2-diol, (1S, 4R)-1-hydroxy-2-oxolimonene, and (3R)-3-isopropenyl-6-oxoheptanoate were intermediates in the (4R)-limonene degradation pathway. The opposite enantiomers [(1R,2R,4S)-limonene-1,2-diol, (1R, 4S)-1-hydroxy-2-oxolimonene, and (3S)-3-isopropenyl-6-oxoheptanoate] were found in the (4S)-limonene degradation pathway, while accumulation of (1R,2S,4S)-limonene-1,2-diol from (4S)-limonene was also observed. These results show that R. erythropolis DCL14 metabolizes both enantiomers of limonene via a novel degradation pathway that starts with epoxidation at the 1,2 double bond forming limonene-1,2-epoxide. This epoxide is subsequently converted to limonene-1,2-diol, 1-hydroxy-2-oxolimonene, and 7-hydroxy-4-isopropenyl-7-methyl-2-oxo-oxepanone. This lactone spontaneously rearranges to form 3-isopropenyl-6-oxoheptanoate. In the presence of coenzyme A and ATP this acid is converted further, and this finding, together with the high levels of isocitrate lyase activity in extracts of limonene-grown cells, suggests that further degradation takes place via the beta-oxidation pathway.
van der Werf
M J
MJ
Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University and Research Centre, Wageningen, The Netherlands. mariet.vanderWerf@imb.ftns.wau.nl
Swarts
H J
HJ
de Bont
J A
JA
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001426:Bacterial Proteins; D001673:Biodegradation, Environmental; D053138:Cyclohexenes; D004851:Epoxide Hydrolases / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000382:microbiology; D000077222:Limonene; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D053138:Cyclohexenes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013729:Terpenes; D000077222:Limonene; D000429:Alcohol Oxidoreductases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; C119722:limonene-1,2-diol dehydrogenase; D010105:Oxygenases; C119723:1-hydroxy-2-oxolimonene 1,2-monooxygenase; C119721:limonene 1,2-monooxygenase; D004851:Epoxide Hydrolases; C115926:limonene-1,2-epoxide hydrolase