title: An improved growth medium for Flavobacterium psychrophilum.
abstract: Supplementing cytophaga agar and broth with 0.5 g l-1 each of D(+) galactose, D(+) glucose, L-rhamnose and skimmed milk gave a dramatic improvement in the isolation of the fish pathogen Flavobacterium psychrophilum. By means of spread-plating, approximately double the number of colonies of larger size were obtained on the improved medium compared to cytophaga agar alone. In supplemented cytophaga broth, growth of Fl. psychrophilum was more rapid and generated greater biomass.
authors.affiliation: Department of Biological Sciences, Heriot-Watt University, Edinburgh, UK.
authors.forename: H
authors.initials: H
authors.lastname: Daskalov
authors.forename: D A
authors.initials: DA
authors.lastname: Austin
authors.forename: B
authors.initials: B
authors.lastname: Austin
mesh_terms: D000818:Animals; D003470:Culture Media; D005393:Fish Diseases / Q000382:microbiology*; D005417:Flavobacterium / Q000254:growth & development* / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D017686:Oncorhynchus mykiss / Q000382:microbiology*
chemical_list: D003470:Culture Media