Content and structure of ceramide and sphingomyelin and sphingomyelinase activity in mouse hepatoma-22.
The relative content of phosphatidylcholine is lower and that of sphingomyelin is higher in transplantable fast growing mouse hepatoma-22, thus decreasing their ratio approximately 2.5-fold versus normal liver. The ceramide content and the neutral sphingomyelinase activity is markedly higher (3- and 6.5-fold, respectively), whereas the acid sphingomyelinase activity is 4-fold lower in hepatoma-22 versus normal liver. The content of saturated fatty acids in ceramide and sphingomyelin of hepatoma-22 is higher than in normal liver. All sphingolipids of hepatoma-22 contain a considerable amount (25-37%) of sphinganine (dihydrosphingosine) along with sphingenine (sphingosine), whereas sphingolipids of normal liver contain predominantly sphingenine (over 95%). These results indicate that the activity of enzymes involved in sphingolipid biosynthesis and catabolism is disturbed in the transplantable mouse hepatoma-22 compared to normal liver.
Rylova
S N
SN
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117871, Russia. dyatl@ibch.siobc.ras.ru.
Somova
O G
OG
Zubova
E S
ES
Dudnik
L B
LB
Kogtev
L S
LS
Kozlov
A M
AM
Alesenko
A V
AV
Dyatlovitskaya
E V
EV
D000818:Animals; D002518:Ceramides / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D015394:Molecular Structure; D011487:Protein Conformation; D013108:Sphingomyelin Phosphodiesterase / Q000737:chemistry / Q000378:metabolism*; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism*
D002518:Ceramides; D013109:Sphingomyelins; D013108:Sphingomyelin Phosphodiesterase