Efficacy and stability of a subunit vaccine based on glycoprotein E2 of classical swine fever virus.
The purpose of this study was to determine the efficacy and stability of an E2 subunit vaccine against classical swine fever virus (CSFV). The vaccine, which contains E2 produced in insect cells by a baculovirus expression vector is a potential marker vaccine, as it allows discrimination between infected and vaccinated pigs. Several vaccination-challenge experiments were performed to determine the dose that protects 95% of the vaccinated pigs (PD95), and to determine the stability and efficacy of the vaccine several months after production. A single vaccination with a vaccine dose of 32 microg E2 - the estimated PD95 - in a water-oil-water adjuvant prevented clinical signs and mortality due to a CSFV challenge-inoculation three weeks after vaccination. Moreover, virus transmission to susceptible sentinel pigs was prevented in nearly all groups of pigs vaccinated with this dose. The vaccine was stable for at least 18 months, and retained its full potency. These findings indicate that the E2 marker vaccine merits further evaluation for suitability for use in a control program during an outbreak of CSF.
Bouma
A
A
Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands. a.bouma@id.dlo.nl
de Smit
A J
AJ
de Kluijver
E P
EP
Terpstra
C
C
Moormann
R J
RJ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D016367:Baculoviridae; D006691:Classical Swine Fever / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission; D006692:Classical Swine Fever Virus / Q000276:immunology* / Q000302:isolation & purification; D007313:Insecta; D013552:Swine; D014162:Transfection; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines; D014766:Viremia / Q000276:immunology / Q000503:physiopathology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus