Expression of an immunoglobulin heavy chain transgene in macrophage as well as lymphocyte lineages in vivo.
A rearranged immunoglobulin heavy chain (IgH) transgene-encoded protein is expressed in macrophage lineage cells, in addition to B and T lineages, in transgenic mouse bone marrow. Peripheral macrophages also express transgenic IgH protein. Mature T cells express lower levels than immature thymocytes. Almost all B220+ cells in the bone marrow express transgenic IgH protein, and this early expression in the B lineage is accompanied by a reduction of cell frequency even in the early B220+ CD43+ BP-1- stages, although it is more prominent in BP-1+ pre-B cells. Thus, an IgH transgene can be expressed not only in lymphoid but also in myeloid cells, although its developmental effects are restricted to the B cell lineage.
Pasare
C
C
National Institute of Immunology, New Delhi, India.
Noggle
S
S
Entringer
M
M
Heinzelmann
A
A
Bansal
P
P
George
A
A
Bal
V
V
Rath
S
S
Durdik
J M
JM
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019076:Transgenes
D007143:Immunoglobulin Heavy Chains