title: Characterization of a Porphyromonas gingivalis gene prtK that encodes a lysine-specific cysteine proteinase and three sequence-related adhesins.
abstract: Porphyromonas gingivalis extracellular arginine- and lysine-specific proteinases have been implicated as major virulence factors in the development of adult periodontitis. We have previously purified a 48-kDa lysine-specific cysteine proteinase, designated PrtK48, from a P. gingivalis W50 cell-associated multiprotein complex. PrtK48 was non-covalently associated with three sequence-related adhesins designated PrtK39, PrtK15 and PrtK44 in the multiprotein complex. In this study we cloned and characterized the gene, designated prtK, that encodes a polyprotein that is post-translationally processed to yield the Lys-specific proteinase PrtK48 and the three sequence-related adhesins PrtK39, PrtK15 and PrtK44.
authors.affiliation: Biochemistry and Molecular Biology Unit, School of Dental Science, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: N
authors.initials: N
authors.lastname: Slakeski
authors.forename: S M
authors.initials: SM
authors.lastname: Cleal
authors.forename: P S
authors.initials: PS
authors.lastname: Bhogal
authors.forename: E C
authors.initials: EC
authors.lastname: Reynolds
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D003546:Cysteine Endopeptidases