Characterization of a hemoglobin-like protein from adult Haemonchus contortus.
A hemoglobin-like protein was purified from supernatants of adult Haemonchus contortus extracts by high-pressure liquid chromatography. The purified protein had an M(r) of 33 kDa as determined by size-exclusion chromatography under non-denaturing conditions and an M(r) of 19 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting the hemoglobin may exist as a dimer. The sequences of 3 peptides resulting from proteolytic digest of the purified protein were determined and demonstrated greater than 50% identity to the globin from Trichostrongylus colubriformis. Adult H. contortus incubated overnight in [3H]leucine, incorporated radioactivity into a peak that coeluted with parasite hemoglobin, indicating the adults synthesize hemoglobin in vitro. The L3-stage lacked hemoglobin, but the L4-stage contained a hemoglobin with an M(r) of 19.6 kDa.
Fetterer
R H
RH
Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Maryland 20705, USA.
Hill
D E
DE
Rhoads
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D006189:Haemonchus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D015801:Helminth Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D007814:Larva / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012756:Sheep / Q000469:parasitology
D015801:Helminth Proteins; D005914:Globins