Effects of a phosphate buffered extracellular (Ep4) solution in preservation and reperfusion injury in the canine liver.
The Ep4 solution, a phosphate buffered extracellular-type solution, is effective in canine lung transplantation following a 96-hour hypothermic (4 degrees C) preservation. In this experiment, we used this solution for liver preservation followed by transplantation. We compared the Ep4 solution with the lactated Ringer's (LR) and the Collins' M (CM) solution (a phosphate buffered intracellular-type solution) in two studies, 1) 48-hour liver preservation, and 2) orthotopic liver transplantation after 5-hour preservation. In the preservation study, purine nucleoside phosphorylase (PNP) levels as a marker of endothelial damage, and alanine aminotransferase (ALT) levels were significantly lower in the livers immersed into the Ep4 solution than in those immersed into other solutions at 36 and 48 hours after preservation. Microscopically, the endothelial injury occurred 24 hours after preservation in the CM solution, and 36 hours after preservation in the LR and Ep4 solutions. In the transplantation study, serum PNP and ALT levels in the livers immersed in Ep4 solution showed a lower tendency compared with those in other solutions at the time of reperfusion, but the histological differences among three groups were not apparent. The present study suggests that the liver can be stored better for a longer time using Ep4 solution than using LR and CM solutions.
Kawashima
Y
Y
The Second Department of Surgery, Gunma University School of Medicine, Maebashi, Japan. ykawashi@sb.gunma-u.ac.jp
Ohwada
S
S
Sakata
K
K
Ohya
T
T
Tomizawa
N
N
Takeyoshi
I
I
Morishita
Y
Y
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D003911:Dextrans / Q000494:pharmacology; D004285:Dogs; D005947:Glucose / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006982:Hypertonic Solutions / Q000494:pharmacology; D007552:Isotonic Solutions / Q000494:pharmacology; D008099:Liver / Q000098:blood supply* / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D016031:Liver Transplantation; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology; D011239:Prednisolone / Q000494:pharmacology; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D000077325:Ringer's Lactate
C024102:Collins' solution; D003911:Dextrans; C087055:Ep4 solution; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D010710:Phosphates; D000077325:Ringer's Lactate; D006493:Heparin; D011239:Prednisolone; D011683:Purine-Nucleoside Phosphorylase; D000410:Alanine Transaminase; D005947:Glucose