title: Detection of the presence of soya protein in milk powder by sodium dodecyl sulfate capillary electrophoresis.
abstract: Two different commercial kits for sodium dodecyl sulfate capillary electrophoresis (SDS-CE) were evaluated for the detection of the presence of soya protein in milk powder. The results obtained showed that SDS-CE allowed the separation of the basic subunits of glycinin and the alpha and alpha' subunits of beta-conglycinin from the main milk protein peaks. However, a detection limit lower than 10% (w/w) of soya protein in total protein could not be achieved. The use of a tetraborate-EDTA sample treatment minimized interferences from milk proteins, allowing the detection of at least 1% (w/w) of soya protein in total protein. The addition of soya protein hydrolysates could not be determined using SDS-CE.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: López-Tapia
authors.forename: M R
authors.initials: MR
authors.lastname: García-Risco
authors.forename: M A
authors.initials: MA
authors.lastname: Manso
authors.forename: R
authors.initials: R
authors.lastname: López-Fandiño
mesh_terms: D002138:Calibration; D003611:Dairy Products / Q000032:analysis*; D004492:Edetic Acid; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D008894:Milk Proteins / Q000032:analysis; D008970:Molecular Weight; D030262:Soybean Proteins / Q000032:analysis*
chemical_list: D008894:Milk Proteins; D030262:Soybean Proteins; D004492:Edetic Acid