title: Immunohistochemical analysis, human papillomavirus DNA detection, hormonal manipulation, and exogenous gene expression of normal and dysplastic human cervical epithelium in severe combined immunodeficiency mice.
abstract: The cervical squamocolumnar junction of normal and dysplastic human xenografts was maintained in SCID-beige mice. Dysplastic tissue maintained a dysplastic morphology, irregular pattern of keratin expression, elevated levels of cellular proliferation, and human papillomavirus type 16 and/or type 18 DNA. Hyperplastic changes of normal xenografts occurred via high-dose estrogen exposure, and through recombinant adenovirus infection, the introduction and stable expression of an exogenous gene was accomplished.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California 92697, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Taylor
authors.forename: K
authors.initials: K
authors.lastname: Tewari
authors.forename: S Y
authors.initials: SY
authors.lastname: Liao
authors.forename: C C
authors.initials: CC
authors.lastname: Hughes
authors.forename: L P
authors.initials: LP
authors.lastname: Villarreal
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002455:Cell Division; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D027383:Papillomaviridae / Q000235:genetics*; D014183:Transplantation, Heterologous; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004958:Estradiol; D007633:Keratins; D001616:beta-Galactosidase