Evidence suggesting protein tyrosine phosphorylation in plants depends on the developmental conditions.
Protein tyrosine phosphorylation plays a central role in a variety of signal transduction pathways regulating animal cell growth and differentiation, but its relevance and role in plants are controversial and still largely unknown. We report here that a large number of proteins from all plant subcellular fractions are recognized by recombinant, highly specific, anti-phosphotyrosine antibodies. Protein tyrosine phosphorylation patterns vary among different adult plant tissues or somatic embryo stages and somatic embryogenesis is blocked in vivo by a cell-permeable tyrosyl-phosphorylation inhibitor, demonstrating the involvement of protein tyrosine phosphorylation in control of specific steps in plant development.
Barizza
E
E
Dipartimento di Biologia, Padua, Italy.
Lo Schiavo
F
F
Terzi
M
M
Filippini
F
F
D000818:Animals; D000906:Antibodies; D010766:Phosphorylation; D063245:Plant Development; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D010944:Plants / Q000196:embryology / Q000378:metabolism*; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*
D000906:Antibodies; D010940:Plant Proteins; D014443:Tyrosine