title: Genetic analysis of large-sized RNA 2 species of a TCM-like tobacco rattle virus source from spinach.
abstract: RNA 2 of a tobacco rattle virus isolate from spinach (TRV SP) resembles that of a recently described isolate from onion (TRV ON), but its ORF 3 is much larger. Naturally infected spinach apparently contains a second TRV SP RNA 2 species with a partially deleted ORF 3 and an RNA 1-like 3'-end which consists of c. 1500 nts and thus is much longer than in any other tobravirus RNA 2 sequenced so far. RNAs 2 of TRV SP and ON are closely related to that of TRV TCM from tulip, but besides the additional ORF 3 they have longer 5'-UTRs with two GCAUA motifs and different coat protein C-termini. The high percentage of amino acid sequence identity in the coat proteins of the TCM-like tobraviruses suggests that they may be considered as strains of a distinct virus species. Possible pitfalls in the use of serology in tobravirus classification are discussed.
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: Koenig
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018724:Spinacia oleracea / Q000821:virology*
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral