Preparation of IgG subclass allotypes from polyclonal IgG.
Two allotypes have been identified for each of the IgG subclasses IgG1, IgG2 and IgG3. These allotypes are referred to as G1m(a) and G1m(f), G2m(n) and G2m(-n), and G3m(g) and G3m(b). Using a pool of normal human serum and a combination of preparative electrophoresis, DEAE ion-exchange and protein A-Sepharose chromatography, it was possible to separate G1m(f) from G1m(a), G2m(-n) from G2m(n) and G3m(g) from G3m(b). Purification of G2m(-n) molecules is of special interest as no genetic marker has been found to identify this allotype.
Oxelius
V A
VA
Department of Pediatrics and Clinical Immunology, University Hospital, University of Lund, Sweden.
D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000097:blood* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood* / Q000302:isolation & purification; D007129:Immunoglobulin Gm Allotypes / Q000097:blood; D012685:Sepharose / Q000031:analogs & derivatives; D013205:Staphylococcal Protein A
D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes; D012685:Sepharose; C042030:Staphylococcal protein A-sepharose; D013205:Staphylococcal Protein A