Microscopic methods for distinguishing among three cell types in TOL plasmid-carrying Pseudomonas putida cultures.
Microscopic methods were developed that enable the sensitive quantification of different cell types that are generated by plasmid instability processes when Pseudomonas putida PaW164 (X+), which carries a TOL plasmid (pWW0-164), is grown in chemostat culture. Cells that have lost the structural TOL genes (X-) or the entire TOL plasmid (X0) can be quantified in a background of 6000 X+ cells using catechol agarose miniplates. X0 cells can be quantified in a background of 3500 X+ or X- cells using carbenicillin agarose miniplates. These methods represent significant improvements in sensitivity over conventional plating methods.
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Department of Environmental Engineering and Sciences, Clemson University, SC 29634-0919, USA.
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Grady
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D001565:Benzoates / Q000378:metabolism; D002228:Carbenicillin / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media; D005798:Genes, Bacterial; D008858:Microscopy, Phase-Contrast; D010403:Penicillin Resistance / Q000235:genetics; D010406:Penicillins / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D014050:Toluene / Q000378:metabolism
D001565:Benzoates; D002396:Catechols; D003470:Culture Media; D010406:Penicillins; D014050:Toluene; D002228:Carbenicillin; C034221:catechol