Amination of polycarbonate surface and its application for cell attachment.
The polycarbonate sheet was modified with ammonia gaseous plasma and characterized by the contact angle measurement and ESCA analysis. The contact angles decreased significantly from 77 degrees to about 20 degrees-40 degrees, indicate that the polycarbonate sheet become more hydrophilic after plasma treatment. The ESCA analysis results showed that the hydrophilicity was mainly derived from the amino groups on the modified surface. In this study, a flow-chamber system was also constructed to evaluate the 3T3 fibroblast cells attachment phenomena on these modified sheets. Before the experimental run, the parameters of inoculated cell number and cell passage were examined previously. The results revealed that these two parameters are independent in shear experiment. And besides, 3-hours plating time has the better adhering fraction. The experimental results showed that the 3T3 fibroblast cells adhesion strength increased significantly on the plasma modified sheet.
Chang
S J
SJ
Institute of Biomedical Engineering, National Yang Ming University, Taiwan, ROC.
Kuo
S M
SM
Lan
J W
JW
Wang
Y J
YJ
D016475:3T3 Cells; D000586:Amination; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D004577:Electron Probe Microanalysis; D008567:Membranes, Artificial; D051379:Mice; D010949:Plasma / Q000737:chemistry; D011076:Polycarboxylate Cement / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; C005506:polycarbonate