Transgenic mice ubiquitously expressing human placental alkaline phosphatase (PLAP): an additional reporter gene for use in tandem with beta-galactosidase (lacZ).
A fundamental keystone of developmental biology has been the growing use of reporter genes in model transgenic systems. Their use has greatly facilitated investigations of cell lineage and cell fate in addition to aiding experiments aimed at determining patterns of gene expression, gene interaction and gene regulation. Through construction of transgenic mice, ubiquitously expressing human placental alkaline phosphatase (PLAP), we demonstrate the suitability of PLAP as a reporter gene for use in conjunction with, or as an alternative to, beta-galactosidase (lacZ). Our findings demonstrate that over-expression of PLAP has no adverse effects on mouse development or viability, despite a widespread pattern of expression. This technology provides a simple yet effective mechanism based on eukaryotic reporter gene technology to facilitate the identification of transgenic cells within complex in vivo systems.
Skynner
M J
MJ
Laboratory of Neuroendocrinology, Babraham Institute, Cambridge, United Kingdom. michael.skynner@bbsrc.ac.uk
Drage
D J
DJ
Dean
W L
WL
Turner
S
S
Watt
D J
DJ
Allen
N D
ND
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism; D002478:Cells, Cultured; D002678:Chimera; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008967:Molecular Biology / Q000379:methods*; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000502:physiology; D010957:Plasmids; D011506:Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
D011506:Proteins; C055324:phospholipase A2-activating protein; D001616:beta-Galactosidase