Inhibition of the expression of VE-cadherin/catenin complex by gamma linolenic acid in human vascular endothelial cells, and its impact on angiogenesis.
Gamma linolenic acid (GLA) has been recently shown to inhibit tumour-induced angiogenesis. The present study investigated the effects of GLA on the HUVEC-specific adhesion. After treatment with GLA, HUVECs decreased the amounts of Triton soluble and insoluble VE-cadherin and beta-catenin and reduced tube formation in matrix in a concentration-dependent manner. An anti-VE-cadherin antibody dissociated HUVECs' colonies and exerted similar inhibitory effects on tube formation of HUVECs. These data indicate that the VE-cadherin/catenins complex is essential for formation and maintenance of new capillaries. It is concluded, therefore, that GLA inhibits tumour-induced angiogenesis partly via the decrease in the expression of VE-cadherin and beta-catenin.
Cai
J
J
University Department of Surgery, University of Wales College of Medicine, Cardiff, CF4 4XN, United Kingdom.
Jiang
W G
WG
Mansel
R E
RE
D015703:Antigens, CD; D015820:Cadherins / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D009369:Neoplasms / Q000098:blood supply; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D015534:Trans-Activators; D051176:beta Catenin; D017965:gamma-Linolenic Acid / Q000494:pharmacology*; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin; D017965:gamma-Linolenic Acid; D000099152:Cadherin 5; C495270:CTNNB1 protein, human