Reversal of methotrexate resistance in human squamous carcinoma cells by SQM1-liposome.
SQM1, a membrane protein associated with methotrexate (MTX) transport was incorporated into unilamellar vesicles, forming. SQM1-Liposome. Human squamous carcinoma of the head and neck (SqCHN) cells resistant to MTX with defective MTX transport and low SQM1 expression were treated with SQM1-Liposome. SQM1 content was found to be increased in these treated SqCHN cells by radioimmunoassay. Concurrent increases in MTX uptake and MTX cytotoxicity in these treated SqCHN cells were found. The ability to reverse MTX-resistance in vitro opens further possibilities for in vivo applications.
Wong
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YC
Division of Hematology/Oncology, San Fernando Valley Program, School of Medicine, University of California, Los Angeles, USA.
Bernal
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SD
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000008:administration & dosage*; D004337:Drug Carriers; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008081:Liposomes; D008727:Methotrexate / Q000493:pharmacokinetics / Q000494:pharmacology*; D009247:NADH, NADPH Oxidoreductases; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D015815:Cell Adhesion Molecules; D004337:Drug Carriers; D008081:Liposomes; C409164:NDUFB7 protein, human; D009247:NADH, NADPH Oxidoreductases; D008727:Methotrexate