The mutagenic and toxic effects of bleomycin and trifluoperazine in Drosophila melanogaster.
The mutagenic and toxic effects of trifluoperazine and bleomycin on Drosophila were investigated in the progenies of males injected with 0.2 microliter of bleomycin and/or trifluoperazine. The Muller-5 method was used to study the induction of complete- and mosaic-sex-linked recessive lethals induced by 0.1 microgram/ml bleomycin and/or 0.1 mM trifluoperazine in the five successive broods, mainly representing the different stages of spermatogenesis. Trifluoperazine increased the induction rate of sex-linked recessive mutations above the spontaneous rates of the control, but these increases were not statistically significant at the 5% level27 in any of the five different broods. Contrary to trifluoperazine, bleomycin significantly (5% level)27 increased the induction rate of the complete sex-linked recessive lethals over those of the control in the meiotic and premeiotic broods C and D, and the meiotic brood E. As with the separate treatment with bleomycin, the frequencies of the complete sex-linked recessive lethals induced by the simultaneous combination treatment of 0.1 microgram/ml bleomycin and 0.1 mM trifluoperazine were significantly higher than those of the control at the 5%27 level, only in the meiotic and premeiotic broods, but they were not significantly higher than those induced by bleomycin treatment alone19. Treatments with 0.1 mM trifluoperazine enhanced the toxicity, sterility and the number of mutated clusters induced by 0.1 mM bleomycin but did not significantly increase the rates of induced lethals over the additive effects of both drugs in the meiotic and premeiotic stages, suggesting no potentiation effects for trifluoperazine over those of bleomycin in Drosophila. Higher concentrations of the two drugs could not be used due to their high toxicity and sterility effects.
Khabour
O F
OF
Department of Biological Sciences, Faculty of Sciences, Yarmouk University, Irbid, Jordan.
Sadiq
M F
MF
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D014150:Antipsychotic Agents / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D005260:Female; D007248:Infertility, Male / Q000139:chemically induced; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D014268:Trifluoperazine / Q000633:toxicity*
D000964:Antimetabolites, Antineoplastic; D014150:Antipsychotic Agents; D009153:Mutagens; D001761:Bleomycin; D014268:Trifluoperazine