[SPIO-enhanced MR angiography for the detection of venous thrombi in an animal model].
OBJECTIVE
An animal model is used to investigate whether MR angiography combined with super-paramagnetic particles of iron oxide (SPIO) is suitable for detecting thromboses.


METHODS
42 rats in groups of 7 each were examined on days 1, 3, 5, 7, 9 and 11, respectively, after mechanical/chemical thrombus induction in a 1.5 Tesla magnet with a FISP sequence (TR/TE/FA 50 ms/6 ms/40 degrees). Imaging was performed before and up to 90 minutes after intravenous injection of 30 mumol FE/kg BW of the experimental SPIO (hydrodynamic diameter, 34 +/- 17 nm LLS; R1 and R2 relaxivity at 0.47 T, 31 and 57 L/(mmol*s)). MIP reconstructions of MR angiographies were submitted to consensus assessment by two examiners. using histology as the gold standard.


RESULTS
The image quality of MIP reconstructions was rated as good in 38 of 42 cases. With regard to thrombotic vessel occlusion, MR angiography coincided with histology in 17 of 42 cases and differed in 25, lumen narrowing being overestimated by MRI in 4 cases and underestimated in 21. In these 21 cases, the histologically observed recanalization could not be detected by MR angiography.


CONCLUSIONS
SPIO-enhanced MR angiography achieves adequate vessel contrast and reliable thrombus detection in animal experiments. Partial volume effects prevent microscopically observed recanalization from being detected by clinical MRI.
Schmitz
S A
SA
Abteilung für Radiologische Diagnostik, Universitäts-Klinikum Benjamin Franklin, Freie Universität Berlin. s.schmitz@medizin.fu-berlin.de
Hansel
J
J
Wagner
S
S
Kressel
M
M
Hüningen
H
H
Wacker
F
F
Wolf
K J
KJ
D000818:Animals; D003287:Contrast Media; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D052203:Ferrosoferric Oxide; D007501:Iron; D007601:Jugular Veins / Q000473:pathology; D018810:Magnetic Resonance Angiography / Q000379:methods* / Q000706:statistics & numerical data; D015588:Observer Variation; D010087:Oxides; D051381:Rats; D013997:Time Factors; D020246:Venous Thrombosis / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology
D003287:Contrast Media; D010087:Oxides; D007501:Iron; D052203:Ferrosoferric Oxide