alpha-D-Glcp-(1<-->1)-beta-D-Galp-containing oligosaccharides, novel products from lactose by the action of beta-galactosidase.
A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)]n-alpha-D-Glcp- (1<-->1)-beta-D-Galp[-(4<--1)-beta-D-Galp]m, with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp- (1<-->1)-beta-D-Galp.
Fransen
C T
CT
Department of Bio-Organic Chemistry, Utrecht University, Netherlands.
Van Laere
K M
KM
van Wijk
A A
AA
Brüll
L P
LP
Dignum
M
M
Thomas-Oates
J E
JE
Haverkamp
J
J
Schols
H A
HA
Voragen
A G
AG
Kamerling
J P
JP
Vliegenthart
J F
JF
D002240:Carbohydrate Sequence; D007785:Lactose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D001616:beta-Galactosidase / Q000737:chemistry*
C119562:Glcp-(1-1)-Galp; D009844:Oligosaccharides; D001616:beta-Galactosidase; D007785:Lactose