title: Phosphorylation of HPr and Crh by HprK, early steps in the catabolite repression signalling pathway for the Bacillus subtilis levanase operon.
abstract: Carbon catabolite repression (CCR) of Bacillus subtilis catabolic genes is mediated by CcpA and in part by P-Ser-HPr. For certain operons, Crh, an HPr-like protein, is also implicated in CCR. In this study we demonstrated that in ptsH1 crh1 and hprK mutants, expression of the lev operon was completely relieved from CCR and that both P-Ser-HPr and P-Ser-Crh stimulated the binding of CcpA to the cre sequence of the lev operon.
authors.affiliation: Unité de Biochimie Microbienne, CNRS URA 1300, Institut Pasteur, F-75724 Paris, France. iverstra@pasteur.fr
authors.forename: I
authors.initials: I
authors.lastname: Martin-Verstraete
authors.forename: J
authors.initials: J
authors.lastname: Deutscher
authors.forename: A
authors.initials: A
authors.lastname: Galinier
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004794:Enzyme Repression; D015964:Gene Expression Regulation, Bacterial; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; C118042:Crh protein, Bacillus subtilis; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D002244:Carbon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; C110847:HPr kinase; D017346:Protein Serine-Threonine Kinases; D006026:Glycoside Hydrolases; C021512:levanase