Presence of a 20 bp insertion/deletion in the ITS1 region of Verticillium lecanii.
Polymerase chain reaction (PCR) amplification of the complete ribosomal RNA internally transcribed spacer (ITS) region of 36 isolates of Verticillium lecanii and related species gave a single 620 bp product in 31 isolates. Five isolates received as V. lecanii, however, gave a single product of 600 bp. Restriction fragment analysis of the PCR products from all isolates gave consistent patterns for the 31 isolates with a 620 bp product. The five isolates with the 600 bp product showed only minor discrepancies to these, generally related to the size of only one restriction fragment. The total ITS region was sequenced from 10 typical 620 bp isolates and one 600 bp isolate. Sequence variation between the isolates varied from 0 to 14.5%, and the 20 bp size discrepancy was found to relate to an insertion or deletion in the centre of the ITS1 region.
Zare
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CABI Bioscience, Egham, Surrey, UK.
Kouvelis
V N
VN
Typas
M A
MA
Bridge
P D
PD
D001483:Base Sequence; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000032:analysis; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020172:Verticillium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D012331:RNA, Fungal; D012335:RNA, Ribosomal