title: Novel endogenous type D retroviral particles expressed at high levels in a SCID mouse thymic lymphoma.
abstract: A xenograft model of the human disease Langerhans cell histiocytosis (LCH) was investigated with severe combined immunodeficiency (SCID) mice. Transplantation of human LCH biopsy material into SCID mice resulted in the generation of mouse tumors resembling lymphomas. A thymoma cell line (ThyE1M6) was generated from one of these mice and found to display significant levels of Mg2+-dependent reverse transcriptase activity. Electron microscopy revealed particles with type D retroviral morphology budding from ThyE1M6 cells at a high frequency, whereas control cultures were negative. Reverse transcription-PCR of virion RNA with degenerate primers for conserved regions of various mouse, human, and primate retroviruses amplified novel sequences related to primate type D retroviruses, murine intracisternal A particles, Jaagsiekte sheep retrovirus, and murine long interspersed nuclear elements but not other retroviral classes. We demonstrate that these sequences represent a novel group of endogenous retroviruses expressed at low levels in mice but expressed at high levels in the ThyE1M6 cell line. Furthermore, we propose that the activation of endogenous retroviral elements may be associated with a high incidence of thymomas in SCID mice.
authors.affiliation: L.A.R.C.H. Cancer Research Unit, Royal Children's Hospital, Parkville, Victoria 3052, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Ristevski
authors.forename: D F
authors.initials: DF
authors.lastname: Purcell
authors.forename: J
authors.initials: J
authors.lastname: Marshall
authors.forename: D
authors.initials: D
authors.lastname: Campagna
authors.forename: S
authors.initials: S
authors.lastname: Nouri
authors.forename: S P
authors.initials: SP
authors.lastname: Fenton
authors.forename: D A
authors.initials: DA
authors.lastname: McPhee
authors.forename: G
authors.initials: G
authors.lastname: Kannourakis
mesh_terms: D017766:Acid Anhydride Hydrolases; D000293:Adolescent; D000818:Animals; D030121:Betaretrovirus / Q000302:isolation & purification*; D020077:Endogenous Retroviruses / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D051379:Mice; D016513:Mice, SCID; D008854:Microscopy, Electron; D009363:Neoplasm Proteins; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013953:Thymus Neoplasms / Q000821:virology*; D014407:Tumor Cells, Cultured; D014771:Virion / Q000302:isolation & purification*; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D011506:Proteins; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase; D000099170:Bis(5'-adenosyl)triphosphatase