title: Structure of the O-specific polysaccharide of a serologically separate strain Proteus penneri 2 from a new proposed serogroup O66.
abstract: O-specific polysaccharide chain of Proteus penneri strain 2 lipopolysaccharide was studied by full and partial acid hydrolysis, Smith degradation, methylation analysis, and NMR spectroscopy, including two-dimensional rotating-frame NOE spectroscopy (ROESY) and 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments. Together with D-glucose and 2-acetamido-2-deoxy-D-glucose, the polysaccharide was found to contain two rarely occurring sugars, 6-deoxy-L-talose (L-6dTal) and 2,3-diacetamido-2,3,6-trideoxy-L-mannose (L-RhaNAc3NAc), and the following structure of a non-stoichiometrically O-acetylated tetrasaccharide repeating unit was established: [equation: see text] The O-specific polysaccharide studied has a unique composition and structure and, accordingly, P. penneri 2 is serologically separate among Proteus strains. Therefore, we propose for P. penneri 2 a new Proteus O-serogroup O66 where this strain is at present the single representative.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: N P
authors.initials: NP
authors.lastname: Arbatsky
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: F V
authors.initials: FV
authors.lastname: Toukach
authors.forename: H
authors.initials: H
authors.lastname: Moll
authors.forename: K
authors.initials: K
authors.lastname: Zych
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: U
authors.initials: U
authors.lastname: Zähringer
authors.forename: Z
authors.initials: Z
authors.lastname: Sidorczyk
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000032:analysis; D006601:Hexoses; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011511:Proteus / Q000737:chemistry* / Q000235:genetics; D012210:Rhamnose / Q000031:analogs & derivatives; D017421:Sequence Analysis; D012699:Serology
chemical_list: D003837:Deoxy Sugars; D006601:Hexoses; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; C011754:6-deoxytalose; D012210:Rhamnose