Investigations on the species specificity of Mannheimia (Pasteurella) haemolytica serotyping.
Eleven serotypes (1, 2, 5-9, 12-14 and 16) have been demonstrated within Mannheimia haemolytica. Subsequent serotyping of 166 Mannheimia haemolytica-like strains, genetically and phenotyphically distinct from Mannheimia haemolytica, and isolated from ruminants, pigs, hares and rabbits showed that 13.2% were typeable, 19 of which were serotype 11 representing strains now being classified as M. glucosida. In addition, three strains belonged to serotypes 6, 9 and 16, respectively. Additionally, the serotyping results of 98 (P.) haemolytica-like isolates from non-ruminant sources collected by the UK Veterinary Investigation Centres during the period 1982-1996 were investigated. None of these isolates have been kept, making further genetic characterization impossible. Among these isolates, 25.5% were typeable representing serotypes 1, 2, 3, 5, 6, 9, 10, 13 and 15. Substantial evidence has been reported indicating that M. haemolytica-like isolates from non-ruminant sources represent species different from M. haemolytica. The present investigation underlines that serotyping does not represent a reliable method for the identification of M. haemolytica or M. glucosida. These observations emphasize that extended phenotypic and genetic characterization is necessary for the proper identification of these organisms.
Angen
O
O
Danish Veterinary Laboratory, Copenhagen. ang@svs.dk
Quirie
M
M
Donachie
W
W
Bisgaard
M
M
D000818:Animals; D002417:Cattle; D005260:Female; D007793:Lagomorpha; D016978:Mannheimia haemolytica / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D012418:Ruminants; D012703:Serotyping / Q000379:methods; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D006113:United Kingdom