title: Characterization of Staphylococcus epidermidis polysaccharide intercellular adhesin/hemagglutinin in the pathogenesis of intravascular catheter-associated infection in a rat model.
abstract: Biofilm production is thought to be a crucial factor in the ability of Staphylococcus epidermidis to produce a biomaterial-based infection. A rat central venous catheter (CVC)-associated infection model was used to assess the importance of biofilm production, mediated by polysaccharide intercellular adhesin/hemagglutinin (PIA/HA), in the pathogenesis of intravascular catheter-associated infection. PIA/HA-positive S. epidermidis 1457 was significantly more likely to cause a CVC-associated infection (71 versus 14%, P < 0.03) resulting in bacteremia and metastatic disease than its isogenic PIA/HA-negative mutant. These results confirm the importance of biofilm production, mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental CVC-associated infection.
authors.affiliation: Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA. MERUPP@UNMC.EDU
authors.forename: M E
authors.initials: ME
authors.lastname: Rupp
authors.forename: J S
authors.initials: JS
authors.lastname: Ulphani
authors.forename: P D
authors.initials: PD
authors.lastname: Fey
authors.forename: D
authors.initials: D
authors.lastname: Mack
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000209:etiology; D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D004195:Disease Models, Animal; D006388:Hemagglutinins / Q000502:physiology*; D008297:Male; D011135:Polysaccharides, Bacterial / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000209:etiology*; D013212:Staphylococcus epidermidis / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
chemical_list: D006388:Hemagglutinins; D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin