title: Safety and efficacy of two live Pasteurella multocida aro-A mutant vaccines in chickens.
abstract: Two auxotrophic aro-A mutants of Pasteurella multocida designated PMP1 (serotype 1) and PMP3 (serotype 3) were tested as vaccine candidates to protect chickens against fowl cholera. A reliable intratracheal challenge method was established that resulted in > or = 75% mortality in both specific-pathogen-free chickens and commercial broiler breeders 24 hr after challenge. Dose protection studies indicated that at least 10(6) colony-forming units (CFU) of PMP1 and 10(8) CFU of PMP3 were required to provide complete protection against challenge in all birds. Although high doses of 10(9) CFU of the vaccine strains produced some endotoxinlike reactions, lower but protective dose levels produced no clinical sign or lesion in any chicken. Both vaccine strains provided cross-protection with a heterologous challenge strain PM206 (serotype 4). Future studies will examine the duration of protective immunity induced by the two vaccine candidates, PMP1 and PMP3, and cross-protection against other serovars.
authors.affiliation: School of Veterinary Science, University of Melbourne, Victoria, Australia.
authors.forename: P C
authors.initials: PC
authors.lastname: Scott
authors.forename: J F
authors.initials: JF
authors.lastname: Markham
authors.forename: K G
authors.initials: KG
authors.lastname: Whithear
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000276:immunology; D000818:Animals; D001428:Bacterial Vaccines; D002645:Chickens; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control*; D016896:Treatment Outcome; D014613:Vaccines, Attenuated
chemical_list: D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase