[Identification and quantification of exogenous metabolites in biological liquids with new development in NMR spectroscopy in one and two dimensions].
By comparison of urine 1H NMR spectrum from a healthy person with those from poisoned patient, we have displayed one or several signals related to intoxication. In complement, various 1H and 13C NMR sequences in 1 and 2 dimensions (J-resolved, TOCSY, HMBC) were used to identify xenobiotic metabolites and quantify them in urine from patients poisoned with salicylate acetyl, chloroquine, valproïc acid, ethylene glycol and pesticides. NMR techniques provide a rapid determination on a small sample volume without pretreatment.
Azaroual
Laboratoire d'Application RMN, Faculté des Sciences Pharmaceutiques et Biologiques, Lille, France.
Imbenotte
M
M
Cartigny
B
B
Lhermitte
M
M
Vermeersch
G
G
D000293:Adolescent; D000328:Adult; D000700:Analgesics / Q000506:poisoning; D000927:Anticonvulsants / Q000506:poisoning; D000962:Antimalarials / Q000506:poisoning; D001241:Aspirin / Q000031:analogs & derivatives / Q000506:poisoning; D002738:Chloroquine / Q000506:poisoning; D005260:Female; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000506:poisoning; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011041:Poisoning / Q000175:diagnosis* / Q000378:metabolism / Q000652:urine*; D015203:Reproducibility of Results; D014635:Valproic Acid / Q000506:poisoning
D000700:Analgesics; D000927:Anticonvulsants; D000962:Antimalarials; D014635:Valproic Acid; D002738:Chloroquine; D008239:Lysine; D001241:Aspirin; C010395:acetylsalicylic acid lysinate