Localization of verotoxin receptors in nervous system.
We use immunohistochemistry to show the existence of verotoxin receptor in small sensory neurons in DRG of human, rabbit, rat and mouse. In capillary in nervous system, the verotoxin receptor exists in human and rabbit, but the receptor could not be demonstrated in rat and mouse, by this method. The receptors in sensory neuron of rat and in capillary in rabbit brain are determined as galactosylglobotriaosylceramide (GalGb3) and globotriaosylceramide (Gb3,), respectively. Although verotoxin was reported to bind to glycolipid receptors that possess the terminal disaccharide Galalpha1-4Galbeta (galactobiose), the binding to toxin to galabiosylceramide was half of that of GalGb3 which has galactobiose internally.
Ren
J
J
Department of Neuroscience, Showa College of Pharmaceutical Sciences, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-0042, Japan. miyatake@wellmet.or.jp
Utsunomiya
I
I
Taguchi
K
K
Ariga
T
T
Tai
T
T
Ihara
Y
Y
Miyatake
T
T
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism / Q000494:pharmacology*; D001921:Brain / Q000098:blood supply; D002196:Capillaries / Q000737:chemistry; D000795:Fabry Disease / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology; D005732:Gangliosides / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis; D006463:Hemolytic-Uremic Syndrome / Q000378:metabolism; D006801:Humans; D007790:Lactosylceramides / Q000032:analysis; D051379:Mice; D009475:Neurons, Afferent / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D022622:Shiga Toxin 1; D014281:Trihexosylceramides / Q000032:analysis
D001427:Bacterial Toxins; D005732:Gangliosides; D006017:Glycolipids; D007790:Lactosylceramides; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; D014281:Trihexosylceramides; C018549:globotriaosylceramide; C054370:galabiosylceramide