title: Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5.
abstract: Crystals of the catalytic core domain of a Trichoderma reesei beta-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P21, with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 A, beta = 111.3 degrees, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 A resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 A at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, England.
authors.forename: E
authors.initials: E
authors.lastname: Sabini
authors.forename: A M
authors.initials: AM
authors.lastname: Brzozowski
authors.forename: M
authors.initials: M
authors.lastname: Dauter
authors.forename: G J
authors.initials: GJ
authors.lastname: Davies
authors.forename: K S
authors.initials: KS
authors.lastname: Wilson
authors.forename: M
authors.initials: M
authors.lastname: Paloheimo
authors.forename: P
authors.initials: P
authors.lastname: Suominen
authors.forename: M
authors.initials: M
authors.lastname: Siika-Aho
authors.forename: M
authors.initials: M
authors.lastname: Penttilä
mesh_terms: D002384:Catalysis; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D008361:Mannosidases / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D014242:Trichoderma / Q000201:enzymology*; D044902:beta-Mannosidase
chemical_list: D005656:Fungal Proteins; D008361:Mannosidases; D044902:beta-Mannosidase