title: Detection of methanogenic archaea in seawater particles and the digestive tract of a marine fish species.
abstract: A methanogen-specific nested PCR approach was used to detect methanogenic archaea in seawater particles of the North Sea and the feces and the digestive tract of flounder (Platichthys flesus), a fish found in the North Sea. A number of 16S rDNA sequences with 97.6-99.5% similarity to Methanococcoides methylutens were found in the seawater particles as well as the digestive tract and fecal samples.
authors.affiliation: Laboratory of Microbial Ecology, Center for Ecological and Evolutionary Studies, University of Groningen, Haren, Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: van der Maarel
authors.forename: W
authors.initials: W
authors.lastname: Sprenger
authors.forename: R
authors.initials: R
authors.lastname: Haanstra
authors.forename: L J
authors.initials: LJ
authors.lastname: Forney
mesh_terms: D000818:Animals; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D019605:Euryarchaeota / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005432:Flounder / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D017019:Methanosarcinaceae / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S