title: Synthesis of an antisense oligonucleotide targeted against C-raf kinase: efficient oligonucleotide synthesis without chlorinated solvents.
abstract: It is demonstrated that a solution of dichloroacetic acid in toluene removes dimethoxytrityl groups from the 5'-terminus of an antisense phosphorothioate oligodeoxyribonucleotide (ISIS 5132/CGP69846A) during synthesis on solid support cleanly and efficiently. It is therefore suggested to replace health hazardous dichloromethane which is typically used in oligonucleotide synthesis as solvent for DMTr-removal by toluene.
authors.affiliation: Isis Pharmaceuticals Inc., Carlsbad, CA 92008, USA.
authors.forename: A H
authors.initials: AH
authors.lastname: Krotz
authors.forename: D L
authors.initials: DL
authors.lastname: Cole
authors.forename: V T
authors.initials: VT
authors.lastname: Ravikumar
mesh_terms: D001483:Base Sequence; D002713:Chlorine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000302:isolation & purification; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics*; D012997:Solvents / Q000737:chemistry
chemical_list: D016376:Oligonucleotides, Antisense; D012997:Solvents; D002713:Chlorine; D019908:Proto-Oncogene Proteins c-raf