Binding of phenylbutazone to human serum albumin. Characterization and identification of binding sites.
The quantitative parameters characterizing the binding of phenylbutazone (CAS 50-33-9) to human serum albumin are determined using the circular dichroism titration method and a combined mathematical approach. The values of affinity constants obtained are in good agreement with those found in the literature. The characterization and identification of binding sites is also performed. A conclusion is derived that phenylbutazone can be used as a model compound for investigation of ligand-macromolecule interactions.
Russeva
V
V
Department of Chemistry, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.
Mihailova
D
D
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D001665:Binding Sites; D002942:Circular Dichroism; D006801:Humans; D007700:Kinetics; D010653:Phenylbutazone / Q000097:blood*; D011485:Protein Binding; D012709:Serum Albumin; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012709:Serum Albumin; D010653:Phenylbutazone