Growth kinetics of genetically engineered E. coli DH 5 cells in artificial cell APA membrane microcapsules: preliminary report.
This paper describes the growth kinetics of genetically engineered E. coli DH5 cells inside the APA membrane artificial cells. The APA microcapsule membrane found does not significantly affects the growth of the encapsulated E. coli DH5 cells. The total protein production of the E. coli DH5 cells inside the APA microcapsules were not significantly different from that of the bacterial cells grown in the free bacterial media. The result also show that the log phase APA artificial cells containing genetically engineered E. coli DH5 would be highly effective for the conversion of various external metabolites.
Prakash
S
S
Artificial Cells & Organ Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Chang
T M
TM
D000464:Alginates; D002214:Capsules; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D005818:Genetic Engineering; D007700:Kinetics; D008567:Membranes, Artificial; D011107:Polylysine / Q000031:analogs & derivatives; D013997:Time Factors
D000464:Alginates; D002214:Capsules; D003470:Culture Media; D008567:Membranes, Artificial; C051546:alginate-polylysine-alginate; D011107:Polylysine