title: Comparative studies of Nsc and Fmoc as N(alpha)-protecting groups for SPPS.
abstract: 2-(4-Nitrophenyl)sulfonylethoxycarbonyl (Nsc) is an alternative base-labile N(alpha)-protecting group to 9-fluorenylmethoxycarbonyl (Fmoc) for amino acids. The UV spectrum of the Nsc group exhibits moderate absorption at 380 nm which is excellent for real-time monitoring of the deprotection process. It also decreases the rearrangement of X-Asp, which can be a serious problem in SPPS.
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D001426:Bacterial Proteins / Q000138:chemical synthesis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D019332:Endothelin-1; D016232:Endothelins / Q000138:chemical synthesis; D005449:Fluorenes / Q000737:chemistry*; D006801:Humans; D009578:Nitrobenzenes / Q000737:chemistry; D010452:Peptide Biosynthesis; D011498:Protein Precursors / Q000138:chemical synthesis; D013373:Substance P / Q000138:chemical synthesis; D013997:Time Factors