Amplified-fragment length polymorphism analysis versus macro-restriction fragment analysis for molecular typing of Streptococcus pneumoniae isolates.
Forty-eight pneumococci were genotyped by on-line laser fluorescence amplified-fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE) analysis of chromosomal restriction fragments. Overall, the data generated by the two methods corresponded well. However, with AFLP, clusters were delineated at a higher similarity level, and isolate differentiation was more pronounced. AFLP and PFGE were equally efficient for assessing intraserotype diversity. We conclude that AFLP is a useful alternative to PFGE.
D000465:Algorithms; D001530:Belgium; D002876:Chromosomes, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005602:France; D005838:Genotype; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification