Haploid male germ cells show no susceptibility to transformation by simian virus 40 large tumour antigen in transgenic mice.
Cell-type specific tumorigenesis can be induced in transgenic mice by the directed expression of simian virus 40 (SV 40) large tumour antigen (TAg). In an attempt to determine the susceptibility of haploid male germ cells to neoplastic transformation by this oncogene, transgenic mice were generated that harboured a chimeric gene composed of the SV40 T antigen genes fused to the 2.3-kb 5' flanking sequences of the rat proacrosin gene. It was previously shown that this regulatory sequence is able specifically to direct the expression of CAT reporter gene in male germ cells with the onset of translation in early haploid male germ cells. The transgene showed regulated expression in male germ cells. Although T antigen immunostaining was detected specifically in spermatids, no testicular pathology was observed. This indicates that spermatids show no susceptibility to transformation by oncogene TAg. However, in about 10% of animals of two independent transgenic lines, we could find non-testicular tumours in abdomen with a sarcoma-like structure in advanced age which showed SV40 TAg expression.
D000008:Abdominal Neoplasms / Q000473:pathology / Q000821:virology; D000176:Acrosin / Q000235:genetics; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D017930:Genes, Reporter; D006238:Haploidy; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012513:Sarcoma, Experimental / Q000473:pathology / Q000821:virology; D013539:Simian virus 40 / Q000235:genetics*; D013087:Spermatids / Q000166:cytology; D013090:Spermatocytes / Q000166:cytology; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*; D013737:Testis / Q000166:cytology* / Q000502:physiology