[Regulation of traditional Chinese medicine formulae bailong on cAMP-PKA and DAG-PKC signal pathways of human gastric cancer cells].
OBJECTIVE
To evaluate the effects of traditional Chinese medicine formulae (Bailong) on cAMP/PKA and diacylaglycerol (DAG) protein kinase (PKC) pathways of the MGc80-3 cells.


METHODS
cAMP level, DAG content and activities of PKA and PKC were measured in different groups: (1) control, (2) 1.8 mg/ml Bailong, (3) 1.8 mg/ml Bailong +20 ug/ml PKA inhibitor, (4) 5 mumol/L PKC inhibitor.


RESULTS
When MGc80-3 cells were in treatment with Bailong for 3 hours, cAMP level and PKA activity were 11.27%, and 19.7% higher than that of the control, while DAG content and PKC activity were 47.0% and 64.2% lower than that of the control; When PKC pathway was blocked by PKC inhibitor GF-109203X, cAMP level and PKA activity were increased by 78.8% and 33.5% as against that of the control, while the DAG content and PKC activity were decreased by 40. 3% and 56.3% respectively. When MGc80-3cells were treated with Bailong, and at the same time, blocked PKA pathways by PKA inhibitor, cAMP level and PKA activity were decreased by 46.0% and 28.9%, on the other hand, DAG content and PKC activity were increased by 50.7% and 51.6% as against that of the Bailong group.


CONCLUSIONS
(1) There was relationship of causes and result between differentiation of MGc80-3 cells and the signal pathways. (2) Results of this study were similar to that of hexamethylenebisacetamide (HMBA). It is shown that the two signal systems are the foundation of regulative effects of Chinese medicine formulae Bailong or HMBA on proliferation and differentiation in MGc80-3 cells.
D002288:Adenocarcinoma, Mucinous / Q000378:metabolism* / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism