Hot water extract of bark of Nikko maple (Acer nikoense) induces apoptosis in leukemia cells.
In screening for antitumor constituents in traditional crude drugs, we used three cultured cell lines: mouse leukemia P388 cells, doxorubicin-resistant P388 cells and leczyme (catalytic lectin)-resistant P388 cells. The hot water extract (HWE) of the bark of Nikko maple (Acer nikoense) showed concentration-dependent inhibitory effects on the growth of these three cell lines. DNA fragmentation and morphological changes, accompanied by condensed and fragmented nuclei, were observed in the leukemia cell lines cultured with HWE of the bark of Nikko maple. Treatment with this HWE increased the expression of sialylated glycoconjugates on the apoptotic cells. These results suggest that HWE induces cell death via apoptosis in vitro.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006001:Glycoconjugates / Q000378:metabolism; D006358:Hot Temperature; D007941:Leukemia P388 / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D014197:Trees / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects; D014867:Water / Q000737:chemistry