title: Comparisons among pimonidazole binding, oxygen electrode measurements, and radiation response in C3H mouse tumors.
abstract: Pimonidazole binding was compared with oxygen electrode measurements and with measurements of the radiobiologically hypoxic fraction in C3H mammary tumors in which oxygenation was manipulated by means of subjecting tumor-bearing CDF1 mice to air breathing, carbogen breathing, oxygen breathing, hydralazine injection or tumor clamping. Hypoxia measured by pimonidazole binding could be correlated with both pO2 (r2 = 0.81) and radiobiologically hypoxic fraction (r2 = 0.85) in this system. The scope and limitation of pimonidazole as an immunohistochemical marker for tumor hypoxia is discussed.
mesh_terms: D000818:Animals; D015415:Biomarkers; D015687:Cell Hypoxia; D004566:Electrodes; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply / Q000378:metabolism / Q000532:radiotherapy*; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D009593:Nitroimidazoles / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis*; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*