Anti-P-selectin antibody attenuates rat brain ischemic injury.
We examined a protective effect of anti-P-selectin monoclonal antibody against rat ischemic brain injury with 24 h of middle cerebral artery occlusion (MCAO). Anti-rat P-selectin monoclonal antibody, was intravenously injected at a dose of 1 mg/kg at 5 min before MCAO. Control animals received the same volume of vehicle. MCAO was accomplished by an insertion of nylon thread with silicone coating for 24 h. Application of anti-P-selectin antibody significantly reduced infarct size and brain water content at 24 h of MCAO. Although leukocyte infiltration was not normally detected, it became remarkably evident at 1 day of MCAO. However, treatment with ARP 2-4 significantly reduced the number of leukocytes. These results demonstrated that administration of monoclonal antibody against P-selectin attenuated infarct size and brain edema, which was associated with reduction of leukocyte infiltration.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001157:Arterial Occlusive Diseases / Q000276:immunology / Q000628:therapy; D001923:Brain Chemistry; D001929:Brain Edema / Q000276:immunology / Q000628:therapy; D002545:Brain Ischemia / Q000276:immunology* / Q000628:therapy*; D002544:Cerebral Infarction / Q000276:immunology / Q000628:therapy; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008297:Male; D019007:P-Selectin / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar