Epidermal growth factor and leukemia inhibitory factor levels in follicular fluid. Association with in vitro fertilization outcome.
OBJECTIVE
To determine the levels of epidermal growth factor (EGF) and leukemia inhibitory factor (LIF) in follicular fluid, if any, and to assess the association of these cytokines with the outcome of in vitro fertilization (IVF).


METHODS
EGF and LIF levels determined by enzyme-linked immunosorbent assay in 60 preovulatory follicular fluids were compared with 25 IVF outcomes.


RESULTS
Immunoreactive EGF and LIF could be detected in human follicular fluid. Levels of these cytokines were similar in FF obtained from follicles that resulted in fertilized oocytes and those that did not. EGF levels were significantly lower in patients establishing a pregnancy as compared to patients achieving no pregnancy (P < .007). LIF levels were similar in both groups of patients.


CONCLUSIONS
EGF appears to be associated with IVF outcome.
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000276:immunology; D004958:Estradiol / Q000032:analysis / Q000276:immunology; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry* / Q000276:immunology; D006131:Growth Inhibitors / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007246:Infertility / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000032:analysis* / Q000276:immunology; D008297:Male; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D011374:Progesterone / Q000032:analysis / Q000276:immunology; D013076:Sperm Count; D013081:Sperm Motility