[Effects of low-molecular-weight Rehmannia glutinosa polysaccharides on p53 gene expression].
OBJECTIVE
To study the effect of low-molecular-weight Rehmannia glutinosa polysaccharides (LRPS) on p53 gene expression.


METHODS
The level of p53 gene expression was determined by quantitative polymerase chain reaction and autoradiography.


RESULTS
The levels of p53 mRNA in Lewis lung cancer tissue were 0.46, 1.52, 1.48, and 1.60 respectively after saline, LRPS 20 and 40 mg.kg-1, and cyclophosphamide 10 mg.kg-1, which were the effective doses inhibiting the growth of tumor tissue cells in vivo. LRPS markedly increased p53 gene expression.


CONCLUSIONS
LRPS effect on p53 gene expression is one of the mechanisms of antitumor activity.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D018827:Carcinoma, Lewis Lung / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D019684:Magnoliopsida / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics