title: Identification of retinoic acid receptor element in human cholesteryl ester transfer protein gene.
abstract: The transcriptional regulation of the human cholesteryl ester transfer protein (CETP) gene by retinoic acid was investigated by a transient transfection assay. A series of deleted vectors generated from the 5'-upstream region (3434 bp) of the CETP gene linked to the luciferase reporter gene was individually transfected to HepG2 cells. Promoter analyses revealed essential regulatory machinery in the -110/-40 region of the upstream sequence of the human CETP gene. When the cells transfected with the reporter vectors were stimulated with all-trans retinoic acid (tRA), the hormone response was drastically reduced when the -165/-110 region was deleted, thereby suggesting that there may be a retinoic acid receptor element (RARE) in the region. A footprinting analysis showed that the DNA segment at the -165/-134 is protected by the HepG2 nuclear extract. A competition analysis on the gel mobility shift assay using consensus RARE and a purified retinoic acid receptor confirmed the -165/-134 region as being RARE.
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D053480:Cholesterol Ester Transfer Proteins; D018983:DNA Footprinting; D017931:DNA Primers; D004586:Electrophoresis / Q000379:methods; D006023:Glycoproteins; D006801:Humans; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D014162:Transfection