title: Pch2 links chromatin silencing to meiotic checkpoint control.
abstract: The PCH2 gene of Saccharomyces cerevisiae is required for the meiotic checkpoint that prevents chromosome segregation when recombination and chromosome synapsis are defective. Mutation of PCH2 relieves the checkpoint-induced pachytene arrest of the zip1, zip2, and dmc1 mutants, resulting in chromosome missegregation and low spore viability. Most of the Pch2 protein localizes to the nucleolus, where it represses meiotic interhomolog recombination in the ribosomal DNA, apparently by excluding the meiosis-specific Hop1 protein. Nucleolar localization of Pch2 depends on the silencing factor Sir2, and mutation of SIR2 also bypasses the zip1 pachytene arrest. Under certain circumstances, Sir3-dependent localization of Pch2 to telomeres also provides checkpoint function. These unexpected findings link the nucleolus, chromatin silencing, and the pachytene checkpoint.
mesh_terms: D018797:Cell Cycle Proteins; D002466:Cell Nucleolus / Q000737:chemistry / Q000235:genetics; D002843:Chromatin / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000502:physiology*; D006655:Histone Deacetylases; D008540:Meiosis / Q000235:genetics*; D016296:Mutagenesis / Q000502:physiology; D009687:Nuclear Proteins; D011995:Recombination, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D013573:Synaptonemal Complex / Q000235:genetics; D016615:Telomere / Q000737:chemistry / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology