Crystallization and preliminary X-ray analysis of a complex between the Bowman-Birk trypsin inhibitor from barley and porcine pancreatic trypsin.
A 1:2 complex between the Bowman-Birk trypsin inhibitor from barley seeds and porcine pancreatic trypsin has been crystallized at 291 K using polyethylene glycol as precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67. 10, b = 88.38 and c = 203.65 A. The asymmetric unit contains two monomers of the complex, with a corresponding Vm of 2.41 A3 Da-1 and a solvent content of 49%. Native data to 2.2 A resolution have been collected at 100 K using synchrotron X-rays.
D003460:Crystallization; D018360:Crystallography, X-Ray; D001467:Hordeum / Q000737:chemistry*; D008027:Light; D010179:Pancreas / Q000201:enzymology*; D012542:Scattering, Radiation; D014357:Trypsin / Q000737:chemistry*; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry*