Simple and reproducible model of rat spinal cord injury induced by a controlled cortical impact device.
Consistently reproducible experimental trauma was inflicted on the rat spinal cord (L3-L4) employing a controlled cortical impact device. The spinal cord was injured with one of three sizes of chips; thick (3 mm diameter), medium (2 mm diameter), thin (1 mm diameter). Each chip was applied at 1, 2 and 3 mm deformation depths. The correlations of the magnitude of the primary trauma were examined histopathologically. It was found that the extent and intensity of the trauma could be changed by altering the depth of deformation and the chip diameter at a fixed velocity of 4.6 m per sec. The injury caused by the 2 mm diameter chip at 0.5 mm-deformation damaged one to two segments of the perifocal spinal cord. In the surviving animals, the histological changes could be classified as primary, secondary, and late phase changes, and finally the lesion became a cavity. This study reports the application of a controlled cortical impact device to morphological research on a spinal cord injury. We found that the injury by the 2 mm chip at 0.5 mm deformation was the most advantageous and reproducible model for further investigations.
D000818:Animals; D001696:Biomechanical Phenomena; D002540:Cerebral Cortex / Q000293:injuries; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005911:Gliosis; D008297:Male; D009457:Neuroglia / Q000737:chemistry / Q000473:pathology; D010243:Paralysis / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology*