Isotachophoretic determination of 3-methylhistidine.
A capillary isotachophoretic (cITP) method to determine the concentration of 3-methylhistidine (3-MeHis) in meat and meat products is described. A clear separation of the 3-MeHis from histidine, 1-methylhistidine and other components of acidic sample hydrolyzate was achieved within 20 min. Method characteristics (linearity, accuracy, precision and detection limit) were determined. Low laboriousness, sufficient sensitivity and low running cost are the important attributes of cITP method. The developed method was successfully applied to analyses of real samples and used for the determination of lean meat content in meat and meat products.
D019075:Electrophoresis, Capillary / Q000379:methods*; D006639:Histidine / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D008762:Methylhistidines / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity