Capillary electrophoretic method for quality control of phospholipid products.
A practical, low cost capillary electrophoretic method for phospholipid products employed in pharmaceutical, cosmetic and food industries with quality control purposes is described. The phospholipid components of the samples were hydrolyzed prior to their electrophoretic separation. The carrier electrolyte to determine choline, ethanolamine and serine consisted of 5 mM imidazole, pH 3.0 using indirect UV detection at 214 nm. Inositol was quantified employing a buffer of 40 mM sodium borate, pH 10.0 at 40 degrees C and detection at 185 nm. Analytical parameters were evaluated and the results obtained of phospholipid composition in commercial samples from soybeans and animal sources showed acceptable values.
D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D010743:Phospholipids / Q000032:analysis* / Q000592:standards; D011786:Quality Control