Evidence for oestrogen receptor alpha-immunoreactivity in gonadotrophin-releasing hormone-expressing neurones.
Having used the cingulate cortex to demonstrate the validity of our methods for detecting hitherto unrecognized oestrogen receptor alpha (ERalpha)-immunoreactive neurones, we have now employed immunoprecipitation and double-label immunohistochemistry to investigate whether the ERalpha protein is present in gonadotrophin-releasing hormone (GnRH)-containing cells. The immortalized GnRH cell line GT1-7 and GnRH neurones within the rat preoptic area were found to possess ERalpha-immunoreactivity (ERalpha-IR). These observations indicate that oestrogen may regulate the synthesis and release of GnRH by direct actions on GnRH neurones.
D000171:Acrolein; D000818:Animals; D000918:Antibody Specificity; D016477:Artifacts; D002457:Cell Extracts; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D047628:Estrogen Receptor alpha; D005260:Female; D005557:Formaldehyde; D005976:Glutaral; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007150:Immunohistochemistry; D008970:Molecular Weight; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D010052:Ovariectomy; D011108:Polymers; D011233:Precipitin Tests; D011301:Preoptic Area / Q000166:cytology*; D051381:Rats; D011960:Receptors, Estrogen / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results