Salivary diagnosis of measles for surveillance: a clinic-based study in Niterói, state of Rio de Janeiro, Brazil.
This study was designed to investigate whether saliva could be a feasible alternative to serum for the diagnosis of recent measles infection in a clinic setting. Forty-two paired blood and saliva samples collected 1 to 16 days after onset of illness from 29 patients with clinical measles were tested for specific immunoglobulin (Ig) M by antibody-capture radioimmunoassay. Measles IgM was detected in all serum samples and in 39 (92.9%) saliva specimens. Between 1 and 3 weeks after illness onset, virus-specific IgM was detected in 100% of saliva samples. Measles IgM was also detected in 17 saliva specimens, not paired with blood samples, collected from study patients 5 days to 3 weeks after onset. Our results indicate that salivary IgM detection is a suitable non-invasive method for investigation of notified cases under conditions of routine clinic use.
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000097:blood; D007223:Infant; D008297:Male; D008457:Measles / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D012463:Saliva / Q000276:immunology* / Q000821:virology