HPLC method to characterize cyanogen bromide collagen fractions containing pyridinoline groups.
The HPLC method here described allows to separate CNBr collagen peptides within 2.5 h by reversed phase and gradient elution. The method is useful to determine both peptide bond and pyridinoline groups by absorbance spectophotometry. The fractions can be recovered and then submitted to other characterization techniques.
D000596:Amino Acids; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000737:chemistry*; D003488:Cyanogen Bromide