Crystallization and preliminary X-ray studies of metallothionein II from rabbit liver.
Metallothionein II (Mw = 6.8 kDa), induced by cadmium and purified from rabbit liver, has been crystallized in space group P6222 or P6422. The unit-cell parameters were a = b = 113.4, c = 219.1 A, alpha = beta = 90.0, gamma = 120.0 degrees when crystallized from sodium citrate buffer and a = b = 113.4, c = 219.6 A, alpha = beta = 90.0, gamma = 120.0 degrees when crystallized from Tris-HCl buffer. There are 12 molecules per asymmetric unit and the solvent content is about 57%.
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D008099:Liver / Q000737:chemistry*; D008668:Metallothionein / Q000737:chemistry*; D011487:Protein Conformation; D011817:Rabbits