[Bicuculline stimulates the release of adrenocorticotropin (ACTH) from rat anterior pituitary quarters].
The effect of gama-aminobutyric acid (GABA) on release of adrenocorticotropin (ACTH) from anterior pituitary quarters of male SD rats adrenalectomized for 96 hrs was investigated using the perfusion technique in vitro. Application of bicuculline (10(-6)mol/L), a specific GABA-A antagonist, resulted in a significant release of ACTH from perfused anterior pituitary quarters. But the same concentration of bicuculline had no effect on the release of ACTH from dispersed anterior cells of ADX rats in vitro. Taken together, these results suggest that GABA may play an important inhibitory role on ACTH secretion from pituitary in adrenalectomized rats.
D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D002478:Cells, Cultured; D018756:GABA Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley