An optical recording system based on a fast CCD sensor for biological imaging.
This paper presents technical details, hardware and software of a complete imaging system which uses a fast CCD sensor and a 41 Msample/s A/D converter to acquire full-frame 12 bit/pixel digitized images with a time resolution of 1.25 ms/image. This apparatus permits to resolve intracellular Ca2+ gradients in individual cells as well as the spatio-temporal pattern of neural activity of cell assemblies in neural tissue.
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism; D004594:Electrophysiology; D005456:Fluorescent Dyes / Q000378:metabolism; D006168:Guinea Pigs; D006624:Hippocampus / Q000378:metabolism; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D009930:Organic Chemicals; D051381:Rats; D013997:Time Factors