Determination of aminoglutethimide enantiomers as dansyl derivative in human plasma by HPLC with fluorescence detection.
Determination of aminoglutethimide enantiomers as a dansyl derivative in plasma by HPLC has been achieved using cellulose tris(3,5-dimethylphenyl carbamate) chiral stationary phase known as Chiralcel OD, and a mobile phase consisted of ethanol-cyclohexane-methanol (95:5:2 v/v/v). The limit of detection for each enantiomer of aminoglutethimide using fluorescence detector was 20 ng ml-1.
D000616:Aminoglutethimide / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis; D005453:Fluorescence; D006801:Humans; D013237:Stereoisomerism