title: Comparative analysis of Otx2, Gbx2, Pax2, Fgf8 and Wnt1 gene expressions during the formation of the chick midbrain/hindbrain domain.
abstract: The patterns of the Gbx2, Pax2, Wnt1, and Fgf8 gene expression were analyzed in the chick with respect to the caudal limit of the Otx2 anterior domain, taken as a landmark of the midbrain/hindbrain (MH) boundary. The Gbx2 anterior boundary is always concomitant with the Otx2 posterior boundary. The ring of Wnt1 expression is included within the Otx2 domain and Fgf8 transcripts included within the Gbx2 neuroepithelium. Pax2 expression is centred on the MH boundary with a double decreasing gradient. We propose a new nomenclature to differentiate the vesicles and constrictions observed in the avian MH domain at stage HH10 and HH20, based on the localization of the Gbx2/Otx2 common boundary.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000032:analysis / Q000378:metabolism*; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000196:embryology*; D008954:Models, Biological; D008957:Models, Genetic; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D051857:Otx Transcription Factors; D051762:PAX2 Transcription Factor; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000378:metabolism*; D012249:Rhombencephalon / Q000196:embryology*; D013997:Time Factors; D014018:Tissue Distribution; D015534:Trans-Activators / Q000032:analysis / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism*; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins