Antimutagenic effect of plant flavonoids in the Salmonella assay system.
The antimutagenic effects of 27 kinds of plant flavonoids on the mutagenicity of aflatoxin B1(AFB1) and N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in Salmonella typhimurium TA100 were investigated. In the mixed applications of AFB1 (1 microgram/plate) with the flavonoids (300 micrograms/plate) in the presence of a mammalian metabolic activation system (S9 mix), chrysin, apigenin, luteolin and its glucoside, kaempferol, fisetin, morin, naringenin, hesperetin, persicogenin, (+)-catechin and (-)-epicatechin showed the antimutagenic effect against AFB1 with more than 70% inhibition rate. A little or no antimutagenicities except flavone against MNNG (0.5 microgram/plate) were observed. For the antimutagenicity of the flavonoids on AFB1, the flavonoid structure that contains the free 5-, 7-hydroxyl group seemed to be essential. However, saturation of the 2,3-double bond or elimination of the 4-keto group did not affect the activity.
D016604:Aflatoxin B1 / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002392:Catechin / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D066298:In Vitro Techniques; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics