title: Quantitative aspects of the use of bacterial chloramphenicol acetyltransferase as a reporter system in the yeast Saccharomyces cerevisiae.
abstract: The quantitative aspects of the use of the reporter system chloramphenicol acetyltransferase (CAT) has been evaluated in the yeast Saccharomyces cerevisiae. It was found that the CAT activity measured with a radiosotopic fluor diffusion assay was strongly dependent on the amount of yeast extract applied, both when CAT was expressed endogenously and when a purified Escherichia coli enzyme was investigated. Desalting the yeast extract by gel filtration partly eliminated the problem, indicating that some low-molecular-weight compound was involved in the phenomenon. However, the extract still exhibited stability problems on ice. An immunological CAT assay was tested and found to yield satisfactory quantitative result.
mesh_terms: D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D004790:Enzyme Induction; D017930:Genes, Reporter; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D009997:Osmotic Pressure; D011865:Radioisotope Dilution Technique; D012441:Saccharomyces cerevisiae / Q000235:genetics*