A new injectable calcium phosphate biomaterial for immediate bone filling of extraction sockets: a preliminary study in dogs.
BACKGROUND
Many different bone substitutes, such as autografts, allografts or synthetic biomaterials have been proposed to restore alveolar bone loss and support efficient placement of dental implants. This experimental study evaluated the osteoconductive properties of an injectable bone substitute (IBS) composed of a polymeric carrier and a calcium phosphate mineral phase, used to fill mandibular and maxillary canine extraction sockets.


METHODS
The polymer was a cellulose derivative (methyl-hydroxy-propyl-cellulose, MHPC), and the mineral phase consisted of granules of biphasic calcium phosphate (BCP) ceramics 200 to 500 microm in diameter. Mandibular and maxillary premolars extracted from 3 dogs (a total of 60 extraction sites) were immediately treated with the IBS or left unfilled as control sites. Animals were sacrificed 3 months after implantation and all extraction sockets were prepared for histological evaluation.


RESULTS
Qualitative histological studies showed that the IBS was able to support the extensive apposition of well-mineralized newly formed lamellar bone over the entire socket surface and appeared to prevent alveolar ridge bone loss in treated extraction sites. Quantitative evaluation showed that the amount of newly formed bone was significantly higher in mandibular than maxillary extraction sockets for both treated and control sites.


CONCLUSIONS
An injectable bone substitute composed of a polymeric carrier and calcium phosphate was effective in enhancing the bone fill of extraction sockets. This approach may prove promising for periodontal lesions. The material expressed osteoconductive capacities, and the biological properties of the mineral phase were conserved.
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000008:administration & dosage / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000494:pharmacology*; D002516:Ceramics; D004285:Dogs; D004337:Drug Carriers; D005260:Female; D065347:Hypromellose Derivatives; D007267:Injections; D008747:Methylcellulose / Q000031:analogs & derivatives; D010865:Pilot Projects; D018709:Statistics, Nonparametric; D014081:Tooth Extraction; D020390:Tooth Socket