[The determination of the site specificity of type-II restriction endonuclease in Streptomyces sp. 48].
The activity of restriction endonuclease which splits DNA of lambda and T7 phages into 8 fragments has been detected in the mycelium lysates of the 2-days culture of Streptomyces sp. 48. The fragments dimensions according to the data of agarose gel electrophoresis, coincide with those of AsuII--fragments of DNA of lambda and T7 phages. This allows the studied Ssp48 restrictase to be considered the isoschizomer of AsuII restriction endonuclease.
D017123:Bacteriophage T7 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity