Evidence for extensive distribution of oestrogen receptor alpha-immunoreactivity in the cerebral cortex of adult rats.
The presence of oestrogen receptor alpha (ERalpha)-immunoreactivity (IR) in the cerebral cortex of adult rats has been investigated. Previous studies have reported a negligible presence of ERalpha or ERalpha mRNA in this region of the adult rat brain. We have used immunoprecipitation and immunohistochemistry, with various antibodies and fixatives, to detect this protein in the cingulate cortex. When the tissue was fixed using paraformaldehyde alone only faint ERalpha-IR was observed at this site. In contrast, following fixation either with acrolein (with or without paraformaldehyde) or with a mixture of paraformaldehyde and glutaraldehyde there was extensive ERalpha-IR throughout layers II to VI; this was absent when the antibodies were preincubated with the peptide fragment used in their production. The presence of ERalpha-IR in the nonfixed cingulate cortex of adult rats was confirmed by immunoprecipitation.
D000171:Acrolein; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D016477:Artifacts; D002540:Cerebral Cortex / Q000737:chemistry*; D004396:Coloring Agents; D047628:Estrogen Receptor alpha; D004967:Estrogens / Q000502:physiology; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral; D007150:Immunohistochemistry / Q000379:methods; D008970:Molecular Weight; D010052:Ovariectomy; D011108:Polymers; D011233:Precipitin Tests; D051381:Rats; D011960:Receptors, Estrogen / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results