title: Structural and immunohistological modifications in olfactory bulb of the staggerer mutant mouse.
abstract: In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volume of three out of the six architectonic layers (glomerular, external and internal plexiform), a reduction of glomeruli size, a loss of half the mitral cells and a slight decrease in juxtaglomerular interneuron number. In staggerer, an hypertrophy of glial ensheathing cell processes was especially evident at the level of each glomerulus, whereas the density of the astrocyte network was weaker in the granular layer and the nerve layer not apparently impaired. An immunofluorescent labelling study combined with confocal scanning microscopy was performed in order to identify the cellular type and the differentiation degree of the various elements. Antibodies anti-GFAP, a protein present in both ensheathing cells and astrocytes, and anti-OMP, the specific maturation protein of the nerve layer, were used for that purpose. Data confirmed the reality of the gliosis and the persistence of the sensory component in the mutant. All the structural alterations described in staggerer olfactory bulb were in close agreement with the functional troubles previously recorded. Our results are discussed in connection with the present knowledge on embryonal origin, fetal development and adult cellular renewal of the olfactory bulb.
mesh_terms: D000818:Animals; D015415:Biomarkers; D002452:Cell Count; D002454:Cell Differentiation; D002526:Cerebellar Diseases / Q000235:genetics / Q000473:pathology*; D017353:Gene Deletion; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants / Q000033:anatomy & histology*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009410:Nerve Degeneration / Q000235:genetics / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D009457:Neuroglia / Q000473:pathology; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D009830:Olfactory Bulb / Q000473:pathology*; D051842:Olfactory Marker Protein; D009832:Olfactory Nerve / Q000473:pathology; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency* / Q000235:genetics; D015534:Trans-Activators / Q000172:deficiency* / Q000235:genetics