The efficacy of 5% Sulfamylon solution for the treatment of contaminated explanted human meshed skin grafts.
Large TBSA burns have a deficiency of skin graft donor sites necessitating meshed skin autografts, cultured epithelial autografts or biosynthetic skin substitutes. Because these do not effect immediate complete biological closure of the wound, the burn victim remains at risk for life-threatening infection. Topical antimicrobials can protect colonization of these grafts from becoming invasive sepsis. However, many of these agents are cytotoxic to new partially keratinized epithelial cells. This study using a model of epithelialization kinetics of human meshed skin grafts explanted to athymic 'nude' rats evaluated: (1) the effect of bacterial colonization on the rate of closure of meshed graft interstices; (2) the efficacy of 5% Sulfamylon solution for bacterial control and (3) the effect on interstitial closure rates caused by control of bacterial proliferation. Results showed the rate of interstitial closure was progressive over 7 days in noncontaminated grafts treated with moistened saline dressings. Areas of total closure of a 1:1.5 meshed graft were seen as early as 5 days. When grafts were inoculated with 10(2) or 10(3) Pseudomonas aeruginosa organisms and treated with saline moistened dressings, the resultant bacterial load rose to 10(6) organisms, less than 3% of the interstices closed and grafts were destroyed. With the same organism level of contamination, bacterial levels were eradicated with topical 5% Sulfamylon solution, interstitial closure rates returned to normal and areas of total meshed graft closure were seen by day 4. These data demonstrate the efficacy of 5% Sulfamylon solution on epithelialization kinetics of contaminated meshed skin grafts.
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002056:Burns / Q000382:microbiology / Q000601:surgery*; D004195:Disease Models, Animal; D006085:Graft Survival / Q000187:drug effects; D006801:Humans; D008272:Mafenide / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011923:Rats, Nude; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D016038:Skin Transplantation / Q000379:methods*; D013526:Surgical Mesh; D013530:Surgical Wound Infection / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects