title: Self-interaction of the herpes simplex virus type 1 regulatory protein ICP27.
abstract: The herpes simplex virus type 1 (HSV-1) regulatory protein ICP27 is a nuclear phosphoprotein required for viral lytic infection, which acts partly at the posttranscriptional level to affect RNA processing and export. In the present study, we show that ICP27 can interact with itself in vivo. Immunofluorescent staining of cells expressing both an ICP27 mutant with a deletion of the major nuclear localization signal and wild-type ICP27 showed that the mutant protein was efficiently imported into the nucleus in the majority of the cotransfected cells, suggesting heterodimer formation between the wild-type and mutant proteins. Coimmunoprecipitation experiments using epitope-tagged wild-type ICP27 and a series of ICP27 mutants with deletions and insertions in important functional regions of the protein revealed that the C-terminal cysteine-histidine-rich zinc-finger-like region of ICP27 was required for the self-association. Furthermore the self-association was also shown in yeast using two-hybrid assays, and again, an intact C-terminal zinc-finger-like region was required for the interaction. This study provides biochemical evidence that ICP27 may function as a multimer in infected cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus; D019281:Dimerization; D000939:Epitopes; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D019913:Nuclear Localization Signals; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011233:Precipitin Tests; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D012441:Saccharomyces cerevisiae; D016335:Zinc Fingers