A study of early pregnancy factor activity in the sera of women with trophoblastic tumor.
OBJECTIVE
To detect whether or not the early pregnancy factor (EPF)-like activity, or chaperonin 10, could be in the sera of patients with trophoblastic tumor in order to find another more efficient means to diagnose this kind of tumor.


METHODS
The rosette inhibition assay was used to detect EPF-like activity in 216 sera, collected from patients with gestational trophoblastic tumor, including 47 sera of patients with choriocarcinoma, 68 sera of patients bearing invasive mole, and 101 sera of patients with vesicular mole.


RESULTS
The accuracy of diagnosing malignant trophoblastic tumor by detecting EPF-like activity is 91.3% (105/115), with a false positive rate of 14.58% and a false negative rate of 4.8% by this method. Furthermore, the rosette inhibition titer (RIT) values have significant difference (P < 0.001) between the sera in patients with malignant trophoblastic tumor before treatment and those after treatment.


CONCLUSIONS
This study demonstrated that diagnosis of malignant trophoblastic tumor could be made with an accuracy of 91.3% by detecting EPF-like activity and that EPF-like activity could be used as an indicator to distinguish benign from malignant trophoblastic tumor.
D000328:Adult; D000818:Animals; D018835:Chaperonin 10 / Q000097:blood; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D010455:Peptides / Q000097:blood*; D011247:Pregnancy; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; D014328:Trophoblastic Neoplasms / Q000097:blood / Q000175:diagnosis*; D014594:Uterine Neoplasms / Q000097:blood / Q000175:diagnosis*