title: Cytochrome P450 CYP1A1 enzyme activity and DNA adducts in placenta of women environmentally exposed to organochlorines.
abstract: Organochlorine compounds bioaccumulate in fishing and hunting products included in the daily diet of many coastal populations. Prenatal and perinatal exposure to large doses of PCBs and PCDFs was shown to be deleterious on fetal and neonatal development, but information is scarce regarding possible effects of chronic low-dose exposure. This study investigates biomarkers of early effects in newborns from women exposed to organochlorines through the consumption of species from marine food chains, in two remote coastal regions of the province of Quebec (Canada). A CYP1A1-dependent enzyme activity (EROD) and DNA adducts were measured in placenta samples obtained from 30 women living on the Lower-North-Shore of the St. Lawrence River and 22 Inuit women from Nunavik (Arctic Quebec). These biomarkers were also assessed in 30 women from a Quebec urban center (Sept-Iles) as a reference group. Prenatal organochlorine exposure was determined by measuring these compounds in umbilical cord plasma. The amount of bulky polycyclic aromatic hydrocarbon (PAH)-related DNA adducts was significantly greater in the Lower-North-Shore group than in the reference group. Placental EROD activity and the amount of less bulky (OC-related) DNA adducts were significantly higher in the Nunavik group than in the reference group. For both biomarkers, smoking was found to be an important confounding factor. Organochlorine exposure was significantly associated with EROD activity and DNA adduct levels when stratifying for smoking. This study confirms that CYP1A1 enzyme induction and DNA adducts in placental tissue constitute useful biomarkers of early effects induced by environmental exposure to organochlorines.
mesh_terms: D000704:Analysis of Variance; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis*; D004781:Environmental Exposure; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006801:Humans; D006843:Hydrocarbons, Chlorinated; D007231:Infant, Newborn; D007306:Insecticides / Q000009:adverse effects* / Q000097:blood; D004930:Inuit; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D011247:Pregnancy / Q000097:blood; D011792:Quebec / Q000453:epidemiology; D012907:Smoking