title: Placing the newborn on the maternal abdomen after delivery increases the volume and CD34 cell content in the umbilical cord blood collected: an old maneuver with new applications.
abstract: OBJECTIVE
  Our purpose was to increase the number of the progenitor cells in umbilical cord blood collected for transplantation.
  
  
  METHODS
  We randomly assessed the effect of "upper" and "lower" positions of the newborn on the volume and progenitor cell (CD34(+)) content of the umbilical cord blood collected from 49 healthy, vaginally delivered, term neonates.
  
  
  RESULTS
  Twenty-two collections were performed in the "upper" and 27 in the "lower" position. The volume of umbilical cord blood obtained in the "upper" position was 108.1 +/- 19.1 mL compared with 42.6 +/- 19.5 mL in the "lower" position (P <.0001). Mononuclear cell separation revealed significantly higher numbers of cells in umbilical cord blood obtained in the "upper" group (P <.01). Although the percentage of CD34(+) cells was comparable, the absolute number of CD34(+) cells was significantly higher in the "upper" group because of the larger volume collected (P <.02). At 24 hours after delivery the hemoglobin levels were not significantly different between newborns of the 2 groups.
  
  
  CONCLUSIONS
  Placing the newborn on the maternal abdomen after delivery and before cord clamping may significantly increase the volume of umbilical cord blood collected and therefore the CD34(+) counts that improve transplantation success without placing the mother or the newborn at risk.
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001810:Blood Volume; D002452:Cell Count; D002469:Cell Separation; D036861:Delivery, Obstetric; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007231:Infant, Newborn / Q000502:physiology*; D008297:Male; D011187:Posture; D011247:Pregnancy; D014470:Umbilical Cord