title: Biliary structures lead to tumour recurrences after laser-induced interstitial thermotherapy.
abstract: OBJECTIVE
  Thermal diffusion during laser-induced interstitial thermotherapy (LITT) has not yet been fully investigated in heterogeneous tissue architecture such as liver. LITT was performed on rabbit liver tumours to analyse the role of biliary structures in thermal diffusion.
  
  
  METHODS
  Twenty-four VX2 tumours were grafted onto 12 rabbit livers. The animals were randomly separated into two groups when tumour size reached 8 mm. Thermotherapy was performed by delivering the 830-nm output of a diode laser to the centre of the tumour with a 300-,microm fibre. Irradiation conditions were 1.5 W over 900 sec. On day 7 or 14, the tumours were removed and stained with haematoxylin-eosin and picrosirius red F3BA (PR). Thermal damage was evaluated by PR and electron microscopic examinations.
  
  
  RESULTS
  Among the treated tumours, recurrences were found both at the periphery (one on day 7, seven on day 14) and within the treated area (two on day 7, two on day 14). All recurrences were located in the vicinity of the biliary structures, which are frequently spared from thermal injury.
  
  
  CONCLUSIONS
  Biliary ducts lead to a heat sink, thereby facilitating tumour recurrences.
mesh_terms: D000818:Animals; D001653:Bile Ducts, Intrahepatic / Q000473:pathology* / Q000502:physiology; D003972:Diathermy / Q000379:methods; D066298:In Vitro Techniques; D017075:Laser Coagulation / Q000379:methods*; D008113:Liver Neoplasms / Q000473:pathology* / Q000628:therapy*; D009364:Neoplasm Recurrence, Local / Q000209:etiology* / Q000473:pathology*; D011183:Postoperative Complications; D011817:Rabbits; D014407:Tumor Cells, Cultured / Q000637:transplantation