[Application of biomarkers in homologous clone identification of enterotoxigenic Escherichia coli].
OBJECTIVE
To study the application of multiple molecular biotechniques and multi-biomarkers in homologous clone identification of enterotoxigenic Escherichia coli (ETEC).


METHODS
On the basis of detection and typing for toxigenic genes, plasmid analysis combined with multilocus enzyme electrophoresis (MEE) was used for the first time in the comprehensive research on ETEC homologous clone.


RESULTS
Two hundred and six strains of ETEC could be classified into 93 plasmid types with plasmid profiling, and 140 strains with unique genotype. Those 206 strains of ETEC could be classified into 148 electrophoresis-types by MEE further, and altogether 190 strains with unique genotype, by combination of three methods.


CONCLUSIONS
Multiple molecular biotechniques and multi-biomarkers can provide much more and more reliable information in homologous clone identification.
D003967:Diarrhea / Q000382:microbiology; D004586:Electrophoresis; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005819:Genetic Markers; D006801:Humans; D010957:Plasmids / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid