title: [Studies on cloned genomic differential DNA fragments extracted from esophageal carcinoma and non-cancerous esophageal tissues in Linxian County].
abstract: OBJECTIVE
  To isolate the genes involved in carcinogenesis of esophageal cancer and to provide a molecular marker for screening and early detection of it in susceptible individuals.
  
  
  METHODS
  DNA extracted from 12 matched pairs of esophageal cancer and non-cancerous tissues in patients of Linxian County, Henan Province, a high incidence area for esophageal cancer, and differential DNA fragments were isolated with arbitrary primer polymerase chain reaction (AP-PCR), cloned and sequenced. Restriction endonuclease Dra I and Hind III were used to digest genomic DNA in the pair-matched cancerous and non-cancerous esophageal tissues for Southern blot analysis.
  
  
  RESULTS
  There was a randomly amplified differential DNA fragment of about 1.0 kb in cancerous tissues in three of the twelve cases with esophageal cancer, but no such fragments could be found in corresponding non-cancerous tissues. Sequence analysis showed that no homologous DNA in GeneBank database as same as 11T differential DNA fragments. Southern blot analysis showed that there was significant difference between pair-matched cancerous and non-cancerous tissues.
  
  
  CONCLUSIONS
  11T differential DNA fragment appeared during carcinogenesis of the esophagus, and further investigations were needed to study if it could be regarded as a new kind of oncogene or a biomarker of it.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D004935:Esophageal Diseases / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics*; D004947:Esophagus / Q000378:metabolism*; D006801:Humans; D016555:Matched-Pair Analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA