Immunofiltration: a methodology for preconcentration and determination of organic pollutants.
A new concentration procedure using an immunofiltration-based method is described. The approach enables quantitative determination of organic pollutants by filtering large volumes of sample through a poly(vinylidene difluoride) membrane where antibodies have been immobilized by passive adsorption. The analysis is based on a sequential competitive enzyme immunoassay. A wide range of sample volumes have been tested (0.2-5.0 mL) for each type of antibody. The improvement on the assay sensitivity and specificity achieved by means of this concentration procedure is discussed. Using this technique and the insecticide carbaryl as a model analyte, a concentration factor of at least 13 and a limit of detection of 4.75 ng/L are accomplished. The suitability of this methodology is demonstrated by the quantification of the insecticide in several types of water samples (bottled, estuarine, and physiological-saline solutions) with recoveries ranging between 102 and 111%. This method has proved to concentrate carbaryl directly, in an accurate way, for residue analysis without using organic solvents or any extraction process. Furthermore, this procedure offers the advantages of carrying out in the same system both preconcentration and quantitative determination of the analyte.
D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D000918:Antibody Specificity; D012721:Carbaryl / Q000276:immunology; D004785:Environmental Pollutants / Q000032:analysis*; D005374:Filtration / Q000379:methods*; D007120:Immunochemistry / Q000379:methods*; D008567:Membranes, Artificial; D014873:Water Pollutants / Q000032:analysis