Cryptosporidiosis in immunocompromised patients in a Turkish university hospital.
The prevalence of Cryptosporidiosis in 18 immunosuppressed diarrheic patients was evaluated by examination of fecal samples by direct staining (Modified Kinyoun and Giemsa), direct and indirect immunofluorescence methods. Forty patients (10 nondiarrheic immunosuppressed, 10 nondiarrheic immunocompetent, and 20 diarrheic immunocompetent) were included in the study as the control group. 11 of 18 samples were positive for cryptosporidial oocysts by at least one of the methods. Oocysts were detected in all (n = 7) of the AIDS patients. This high frequency was attributed to a probable nosocomial infection. None of the samples from control subjects were found positive for Cryptosporidium. Our results indicate that Cryptosporidial oocysts should be detected particularly in immunosuppressed patients with diarrhea. Modified Kinyoun staining method is practical and reliable for this purpose. Immunofluorescence staining methods can be applied for confirmation of the results.
D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology; D000328:Adult; D000818:Animals; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000209:etiology; D003458:Cryptosporidium / Q000302:isolation & purification; D003967:Diarrhea / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D015995:Prevalence