Protein folding observed by capillary electrophoresis in isoelectric buffers.
Capillary zone electrophoresis measurements in acidic isoelectric buffers provide a sensitive and rapid method for comparison of the folding and stability of wild type, mutant or post-translationally modified proteins. The potential of the method is illustrated using the small globular protein cytochrome c.
D002021:Buffers; D003574:Cytochrome c Group / Q000737:chemistry*; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry; D017510:Protein Folding; D012260:Ribonucleases / Q000737:chemistry