[The effect of cyclosporine on human endothelial cells in culture].
Cyclosporine A is a selective immunosuppressive agent, which significantly minimises graft versus host disease and graft rejection at transplanted patients. Its pharmacological function is many-sided but it is still the subject of research. The author researched direct acting of Cyclosporine A in the culture of endothelial cells. Homogeneous continuous line of endothelial cells from umbilical line had been exposed to Cyclosporine A in the concentration of 2 mg/ml for seven days. Cyclosporine A inhibited replication and proliferation in the culture of endothelial cells. Some abnormal morphological changes were shown: formation of vesicles, nuclear changes and explicit destruction of the cells. By determination cells' vitality, it was discovered significantly smaller number of cells, in a comparison with control cultures (P < 0.01), while by using of monoclonal antibodies on vWF: Ag, a quantitative change in its expressiveness in relation to the control group was proved.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D014471:Umbilical Veins