[Determination of sex using PCR of the X-Y homologous gene amelogenin].
Sex determination is carried out by simultaneously amplifying a X-specific and Y-specific DNA fragments which are 106 bp and 112 bp long respectively in one PCR reaction using one pair of primers which are specific to the first intron of the X-Y homologous gene Amelogenin PCR products are separated on a PAGE electrophoresis followed by visulization using silver stain. All the blood stain, muscle, saliva and hair samples gave correct sex determination. Less than or as little as 50 pg template DNA and the 16-year-old blood stain all can achieve satisfactory amplification. The results showed that the method discribed in this paper is a rapid, sensitive, reliable and easily manipulated sex test highly adapted to forensic specimen especially those petrified and degraded.
D053523:Amelogenin; D001769:Blood; D001801:Blood Stains; D004247:DNA / Q000032:analysis*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D006197:Hair / Q000737:chemistry; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D014960:X Chromosome; D014998:Y Chromosome