title: Pore-forming properties and antibacterial activity of proteins extracted from epidermal mucus of fish.
abstract: Among several biological functions, the epidermal mucus of fish may play an important role in host defense, particularly in the prevention of colonization by parasites, bacteria and fungi. In previous work, two hydrophobic proteins of 27 and 31 kDa were isolated from carp mucus. This study identified a strong antibacterial activity (0.16-0.18 microM) well correlated with pore-forming properties. Here this work was extended to other fish species, four fresh water fish and one sea water fish. After a first step of purification, water-soluble and hydrophobic material were separated, and both fractions were analyzed by SDS-PAGE and capillary electrophoresis. Only the hydrophobic component induced pore-forming activity, when reconstituted in planar lipid bilayers. This pore-forming activity was well correlated to a strong antibacterial activity against several bacteria strains. These results suggest that fish secrete antibacterial proteins able to permeabilize the membrane of the target cell and thus act as a defense barrier.
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D005399:Fishes / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects; D007476:Ionophores / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D009093:Mucus / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D012867:Skin / Q000378:metabolism* / Q000382:microbiology