Dauricine inhibited L-type calcium current in single cardiomyocyte of guinea pig.
OBJECTIVE
To study the effect of dauricine (Dau) on L-type calcium current in guinea pig ventricular myocytes.


METHODS
Using whole-cell recording method to record L-type calcium current (ICa) in single ventricular cell of guinea pig.


RESULTS
Dau 1, 10, and 100 mumol.L-1 markedly reduced ICa by 15.2% +/- 2.2%, 41% +/- 5%, and 82% +/- 8%, respectively. After washing out, ICa partially recovered. Dau inhibited ICa at 3 Hz and 1 Hz to a similar extent, its effect on ICa appeared to be not frequency-dependent.


CONCLUSIONS
Dau had a calcium channel blocking effect.
D000470:Alkaloids; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D044182:Benzylisoquinolines; D015220:Calcium Channels / Q000187:drug effects*; D006168:Guinea Pigs; D007546:Isoquinolines / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology*; D018408:Patch-Clamp Techniques; D044005:Tetrahydroisoquinolines