title: Low chronic ethanol consumption affects ovulation and PGE synthesis by the cumulus cell masses in mice.
abstract: Central and gonadal function can be affected by chronic consumption of high and moderate doses of ethanol. Few studies have been conducted to determine the effect of ethanol intake at ovarian and gamete level. Previously, we showed that fertilization rates of low chronic ethanol treated female mice were diminished. Also, our recent results indicated that moderate chronic intake of ethanol by immature females could alter the ovulatory quantity and produce morphological alterations in the superovulated oocytes. Furthermore, PGE production by oocyte cumulus complexes (OCCs) was reduced in the females treated with 10% (w/v) ethanol. In the present investigation, we studied the effects of 5% ethanol treatment given to immature mice for 30 days on the quality and quantity of oocytes superovulated at 16 h posthuman chronic gonadotrophin. Treated females had impaired ovulation rates (P < 0.05) as compared to the controls. The percentage of activated and morphologically abnormal oocytes was elevated in the ethanol-treated females (P < 0.05). PGE synthesis by the OCCs was higher than in the controls (P < 0.01). In summary, the administration of long-term ethanol at a relatively low dose to immature females produces decreased ovulation rates, abnormal oocyte morphology with high spontaneous activation and altered levels of PGE production by the oocytes' cumulus complexes. The relationship between the oocyte quality and abnormal synthesis of PGE is discussed.
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010060:Ovulation / Q000187:drug effects*; D011458:Prostaglandins E / Q000096:biosynthesis*