[Mutation of p53 gene in the transformed fibroblasts detected by nonradioisotopic single strand conformation polymorphism analysis].
The human embryonic lung fibroblasts transformation was induced by chemical mutant Glycidyl Methacrylate (GMA) in vitro. Transformed clonies were isolated and then exon 5 and exon 8 of p53 gene were specifically amplified by polymerase chain reaction. Amplified fragments were detected by using silver stained single strand conformation polymorphism analysis. The results showed that exon 8 altered in the transformed cells, indicating that p53 gene played an important role in the human embryonic lung fibroblasts transformation induced by GMA.
D000395:Air Pollutants, Occupational / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic; D004622:Embryo, Mammalian; D004852:Epoxy Compounds / Q000633:toxicity*; D005347:Fibroblasts / Q000166:cytology; D016158:Genes, p53; D006801:Humans; D008168:Lung / Q000166:cytology*; D008689:Methacrylates / Q000633:toxicity*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational