Coinfection of Spodoptera exigua and Spodoptera frugiperda cell lines with the nuclear polyhedrosis viruses of Autographa californica and Spodoptera exigua.
The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) has a broad host range among Lepidoptera. In contrast, the Spodoptera exigua MNPV (SeMNPV) can replicate efficiently only in S. exigua larvae or S. exigua-derived cell lines. In this study, we examined the coinfection of S. exigua Se301 and Spodoptera frugiperda IPLB-SF21AEII (Sf21) cell lines with SeMNPV and AcMNPV recombinant (Ac360-501beta-gal) which was constructed for expression of beta-galactosidase under control of the polyhedrin promoter. Coinfection led to the restriction as the level of late gene expression, nonoccluded virus production, and DNA replication of Ac360-501beta-gal in both Se301 and Sf21 cell lines. In contrast, Ac360-501beta-gal supported the SeMNPV replication in Sf21 cells. Occurrence of recombinants, between Ac360-501beta-gal and SeMNPV, with expanded host range was not observed in coinfected Sf21 cells. This suggests that Ac360-501beta-gal supports the SeMNPV replication through trans-activation.
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D007313:Insecta / Q000821:virology*; D007814:Larva / Q000821:virology; D009693:Nucleic Acid Hybridization; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity / Q000502:physiology*; D018411:Spodoptera / Q000821:virology*; D014762:Viral Interference; D014779:Virus Replication; D001616:beta-Galactosidase / Q000378:metabolism