title: ATF-1 is activated in response to UV irradiation in B16 melanoma cells.
abstract: We have found that the transferrin receptor gene promoter is strongly activated by exposure of B16 melanoma cells to UV light. This is a delayed event occurring more than 6 h after exposure and requires an AP-1/CRE-like element in the promoter as demonstrated by site-specific mutagenesis. UV irradiation enhances the binding of a nuclear factor to this element and supershift analysis demonstrates that this DNA-protein complex involves ATF-1. No other members of either the AP-1 or CREB/ATF families of transcription factors were found to bind to this DNA element in UV-irradiated B16 cells. Western blots show that the level of ATF-1 does not change following exposure to UV light, indicating that the increased binding of this factor is most likely mediated by posttranslational modifications in response to UV-mediated signaling pathways.
mesh_terms: D051697:Activating Transcription Factor 1; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic / Q000528:radiation effects; D011990:Receptors, Transferrin / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays