Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants.
We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006168:Guinea Pigs; D015707:HN Protein / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D006382:Hemadsorption; D006801:Humans; D009500:Neutralization Tests; D011233:Precipitin Tests; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018108:Respirovirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000235:genetics* / Q000276:immunology / Q000502:physiology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology