[Purification of PCR products from paraffin-embedded lung cancer tissue of pneumoconiosis cases].
A method of purification of PCR products from paraffin-embedded human lung tissue by low melting temperature agarose gel electrophoresis was developed. This method increased the specificity of PCR products of DNA from lung tissues with pneumoconiosis. The retrieved DNA fragments can be used for SSCP, sequencing and other molecular biology research.
D016158:Genes, p53; D006801:Humans; D008175:Lung Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D016612:Paraffin Embedding; D011009:Pneumoconiosis / Q000150:complications / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA