title: Inhibition of calcium channels by neurokinin receptor and signal transduction in hamster submandibular ganglion cells.
abstract: Both substance P (SP) and neurokinin A (NKA) are known as neurotransmitters of the submandibular ganglion (SMG) neurons. SP released from collaterals of the sensory nerves also regulates the excitability of SMG neurons. It has recently been shown that neurokinins (NK) inhibit calcium channels in various neurons. In this study, the effects of NK on voltage-dependent calcium channel current (I(Ca)) in SMG cells were investigated using the whole-cell patch-clamp recording method. NK-1 receptor agonist and SP caused inhibition of I(Ca) in SMG cells in a dose-dependent manner. NK-1 receptor agonist inhibited L-, N- and P/Q-type I(Ca) components. GDP-beta-S included in the pipette solution reduced the NK-1 receptor agonist-induced inhibition of I(Ca). In addition, NK-1 receptor agonist-induced inhibition of I(Ca) was reduced by stimulation of protein kinase C (PKC) but not cyclic AMP-dependent protein kinase (PKA). The results provided evidence for a signal transduction pathway in which calcium channel inhibition by NK receptors required activation of G-protein and PKC-affected step phosphorylation in SMG neurons.
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism* / Q000502:physiology; D020864:Calcium Channels, N-Type; D006224:Cricetinae; D004553:Electric Conductivity; D004789:Enzyme Activation / Q000502:physiology; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000502:physiology*; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives; D008297:Male; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques; D011493:Protein Kinase C / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000819:agonists / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013363:Submandibular Gland / Q000294:innervation*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology