A method for maintaining odor-responsive adult rat olfactory receptor neurons in short-term culture.
We report a culture system requiring the addition of freshly made ascorbic acid to the medium, that supports the short-term survival of adult rat olfactory receptor neurons. The cultured neurons exhibit typical voltage-gated currents and are responsive to application of odorants.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D004594:Electrophysiology; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis; D009812:Odorants; D051842:Olfactory Marker Protein; D018034:Olfactory Receptor Neurons; D051381:Rats; D013997:Time Factors; D014404:Tubulin / Q000032:analysis