T cell receptor beta chain lacking the large solvent-exposed Cbeta FG loop supports normal alpha/beta T cell development and function in transgenic mice.
The striking and unique structural feature of the T cell receptor (TCR) beta chain is the bulky solvent-exposed FG loop on the Cbeta domain, the size of almost half an immunoglobulin domain. The location and size of this loop suggested immediately that it could be a crucial structural link between the invariant CD3 subunits and antigen-recognizing alpha/beta chains during TCR signaling. However, functional analysis does not support the above notion, since transgene coding for TCR beta chain lacking the complete FG loop supports normal alpha/beta T cell development and function.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D005434:Flow Cytometry; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D016296:Mutagenesis; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D017384:Sequence Deletion; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection