title: In vitro comparative effect of Cd2+, Ni2+, and Co2+ on mouse postblastocyst development.
abstract: Postblastocyst development of mouse preembryos was studied in vitro in order to determine direct effect of Cd2+, Ni2+, and Co2+ ions on embryogenesis during the peri-implantation stage. Uterine horns were flushed on Day 4 of pregnancy and expanded blastocysts were cultured for 4 days in the presence of micromolar Cd2+ (1.1-26.4), Ni2+ (0. 1-500) or Co2+ (1-200). Area of trophoblast outgrowth was measured and used as a quantitative toxicological endpoint. Hatching, attachment, outgrowth, and formation of inner cells mass were also registered. Significant adverse effect on the development stages were observed at 2.2 microM (Cd2+), at 10 microM (Ni2+), and at 100 microM (Co2+). Cd2+ and Co2+ decreased the area of trophoblast markedly at concentrations of 1.1 and 10 microM, respectively. Ni2+ exposure resulted in a slight increase at 10 microM followed by a marked reduction in the trophoblast area at 250 microM. Reduced proliferative ability of trophoblast cells may point to compromised invasiveness of the embryo. The lowest Cd2+ concentration (1.1 microM=0.25 microg/ml) significantly deteriorating trophoblast development was found to be lower than Cd levels ranging up to 0.512 microg/g, reported in clinical ovarian samples of occupationally nonexposed women. The morphological alteration and loss of cellular contacts in blastocysts induced by exposure to Cd2+, Ni2+, or Co2+ may adversely influence adhesion and recognition events and may disturb aggregation of mononuclear trophoblastic cells to multinucleated cells in the course of peri-implantation in vivo as well.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002104:Cadmium / Q000633:toxicity*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000187:drug effects; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D009532:Nickel / Q000633:toxicity*; D011247:Pregnancy; D013997:Time Factors; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects