Long-term durability of dentin bonds made with a self-etching primer, in vivo.
The long-term durability of bonds between adhesive resins and dentin is of significant importance for the longevity of bonded restorations. We carried out an in vivo one-year study to evaluate the durability of resin-dentin bonds in the oral cavity, as well as to test the hypothesis that the adhesive interface would show morphological changes in vivo over time. Very shallow saucer-shaped dentin cavities were prepared in 12 intact teeth of one Japanese monkey (Macaca fuscata) under general anesthesia. The cavities were restored with Clearfil Liner Bond II and Clearfil Photo Posterior resin composite. The teeth were extracted at three different times: immediately, and 180 and 360 days after placement of the restorations. One day after the monkey was killed, specimens of the three time periods were subjected to the micro-tensile bond test at a crosshead speed of 1 mm/min. The surfaces of the failed bonds were observed under a field emission scanning electron microscope (FE-SEM). Bond strength measurements in this study were successfully performed and were stable at approximately 19 MPa during the one-year testing. Scanning electron microscopic observations of the failed surfaces revealed, at the top of the hybrid layer and within the adhesive resin, porosity which increased over time. Long-term bonds can be assessed in vivo by the combined evaluation of the microtensile bond strength and SEM morphological examination of the adhesive interface.
D000134:Acid Etching, Dental / Q000379:methods / Q000706:statistics & numerical data; D000704:Analysis of Variance; D000818:Animals; D001840:Dental Bonding / Q000379:methods / Q000706:statistics & numerical data; D019232:Dental Restoration Failure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D008251:Macaca; D008334:Mandible; D008422:Materials Testing / Q000379:methods / Q000706:statistics & numerical data; D008437:Maxilla; D008855:Microscopy, Electron, Scanning; D008963:Molar; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors