Preliminary crystallographic studies of a protease resistant botulinum neurotoxin associated protein Hn-33.
Botulinum neurotoxin (BoNT) is one of the most potent toxins known. BoNT is also a food poison, which means that the toxin must survive the protease action and acidity of the gut. A group of neurotoxin-associated proteins which are only beginning to be identified and characterized are believed to be responsible for this protection. Hn-33 is a 33 kDa polypeptide which is a major component of the type A botulinum neurotoxin complex. Crystals of Hn-33 have been grown by vapour-diffusion techniques. They belong to a primitive orthorhombic space group and diffract to a resolution of 2. 6 A, with unit-cell parameters a = 130.3, b = 122.2, c = 37.2 A.
D001426:Bacterial Proteins; D001905:Botulinum Toxins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D010450:Endopeptidases / Q000378:metabolism*; D006388:Hemagglutinins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation