Characterization of the human CR1 gene promoter.
The human CR1 is a single chain membrane glycoprotein that is a member of the group of regulators of the complement activation system. In order to clarify the regulatory mechanisms of human CR1 gene expression, the 5'-flanking region of the human CR1 gene was isolated and its promoter was characterized. The CR1 expression was found to be transcriptionally up-regulated in HL60 cells by stimulation with DMSO. The cloned CR1 gene promoter was sequenced and computer analyzed. The potential promoter region lacks a distinct TATA box sequence. The transcription initiation site was determined by primer extension and several possible regulatory elements for transcription were found in the promoter region.
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017463:Receptors, Complement 3b / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D015854:Up-Regulation / Q000187:drug effects