Biological effects of a bifunctional DNA crosslinker. I. Generation of triradial and quadriradial chromosomes.
Interduplex crosslinks by a bifunctional anthramycin DNA crosslinker produced triradial and quadriradial chromosomes. The crosslinker alkylates guanine at N-2. Bovine chromosomes contain GC-rich density satellite DNAs at the centromeric heterochromatin and is the basis for the formation of triradial and quadriradial chromosomes at the centromeres. The in situ crosslinking of interphase chromosomes indicates that the distance between centromeres is 17.5 A. We conclude that the nuclear matrix associated DNA in the centromeric heterochromatin of interphase chromosomes are positioned close enough for crosslinking to occur. We propose a model for the generation of triradial and quadriradial chromosomes based upon the number of interduplex crosslinks between two chromosomes.
D000818:Animals; D000876:Anthramycin / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D002869:Chromosome Aberrations; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004276:DNA, Satellite / Q000187:drug effects*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping