6-Oxo-PGF1alpha and 8-epi-PGF2alpha in human atherosclerotic vascular tissue.
Isoprostanes are a new family of compounds generated by the free radical catalyzed action on arachidonic acid. Formed during oxidation they have been claimed to be a reliable indicator of in vivo oxidation injury. We assessed the amount of 8-epi-PGF2alpha in human surgical specimens as compared to PGI2 (via its stable metabolite 6-oxo-PGF1alpha), the major compound generated by vascular tissue. 8-epi-PGF2alpha is low in normal vascular tissue as is the 8-epi-PGF2alpha/6-oxo-PGF1alpha ratio. The vessels of smokers in general exhibited an increased 8-epi-PGF2alpha (r=0.82) and a decreased 6-oxo-PGF1alpha (r=0.71). The 8-epi-PGF2alpha/6-oxo-PGF1alpha ratio is, not significantly, increased in vessels derived from hyperlipidemics and hypertensives. These findings indicate that lipid peroxidation occurs within the human arterial wall as evidenced by 8-epi-PGF2alpha, probably further decreasing the synthesis of PGI2 and promoting atherogenic mechanisms.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000367:Age Factors; D000368:Aged; D001161:Arteriosclerosis / Q000378:metabolism*; D001808:Blood Vessels / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism*; D028441:F2-Isoprostanes; D005260:Female; D006801:Humans; D007118:Immunoassay; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged