title: Fibronectin-pluronic coadsorption on a polystyrene surface with increasing hydrophobicity: relationship to cell adhesion.
abstract: Recently, patterned polystyrene surfaces containing hydrophobic (PS) and more hydrophilic (PSox) areas have been shown to be capable of directing cellular growth, which is mainly due to the competitive adsorption of adhesive and antiadhesive molecules. In this article, the competitive adsorption between a pluronic surfactant and fibronectin was studied on homogeneous PS or PSox substrates conditioned with mixtures containing increasing concentrations of one of the two molecules. Radiolabeling and X-ray photoelectron spectroscopy techniques showed that fibronectin adsorption increased on both surfaces if the fibronectin concentrations increased in the conditioning mixture. In contrast, fibronectin adsorption decreased on PSox and did not occur on PS surfaces when pluronic concentrations increased in the coating mixture. A comparison of these data with pheochromocytoma and Schwann cells cultured on patterned surfaces showed that the direction of cell growth on PSox areas depended first on the relative concentrations of the two components in the mixtures, and second, on their ratio; the best concentration ratio probably depends on the cell's ability to recondition its support.
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005353:Fibronectins / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D016716:PC12 Cells; D020442:Poloxamer / Q000737:chemistry* / Q000494:pharmacology*; D011137:Polystyrenes / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells; D013052:Spectrometry, X-Ray Emission; D014407:Tumor Cells, Cultured