Interleukin-1alpha-induced changes in chromium-51 absorption, tissue retention, and urinary excretion in rats.
The effects of interleukin-1 alpha (IL-1alpha) on chromium-51 absorption, tissue retention, and urinary excretion were studied in adult male Sprague-Dawley rats. Ten rats were deprived of food for 12 h, injected intraperitoneally with mouse recombinant IL-1alpha (1 microg/kg of body weight in phosphate-buffered saline [PBS]) or control (0.1% bovine serum albumin [BSA] in PBS). Two hours after dosing with the IL-1alpha, rats were fed 50 microL (200 microCi, 0.36 microg Cr) of 51CrCl3 by micropipet. Blood was collected from the tail at 0.25, 0.5, 1, 2, and 4 h. Six hours after dosing with 51CrCl3, rats were exsanguinated and blood and tissues were sampled. The IL-1alpha significantly decreased chromium-51 in blood, urine, and some tissues compared to the control. The decreased absorption, retention, and urinary excretion of chromium-51 from 51CrCl3 in this study may be due to IL-1alpha-mediated increases in the production of prostaglandins and/or decreased production of gastric acid.
D000818:Animals; D002860:Chromium Radioisotopes / Q000378:metabolism* / Q000652:urine*; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014018:Tissue Distribution