Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3).
Mouse PIAS3 (protein inhibitor of activated STAT3) is a specific inhibitor of STAT3 that downregulates its signaling pathway. Here we report the isolation and chromosome mapping of the human PIAS3 gene. Human PIAS3 cDNA encoded a predicted protein of 619 aa which has 83% overall amino acid identity to the mouse counterpart. Based on polymerase chain reaction assisted analysis of a human/rodent mono-chromosomal hybrid cell panel and a radiation hybrid mapping panel, the human PIAS3 gene was mapped to the chromosome 1q21 region. Mapping of a crucial gene in modulating the STAT3 signaling pathway may provide new clues to the understanding of malignancies or genetic disorders caused by this chromosome region.
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D050796:STAT3 Transcription Factor; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000037:antagonists & inhibitors*