[Effect of yuxintong on ECE, NOS gene expression and immunohistochemical studies on cooper-zinc-SOD in ischemic myocardium in experimental dogs].
OBJECTIVE
To explore the effect of Yuxintong (YXT) on the endothelin converting enzyme (ECE) and the nitric oxide synthase (NOS) gene expression levels and cooper-zinc-superoxide dismutase (SOD) activity.


METHODS
The acute myocardial ischemia models were reproduced in anesthetized dogs by ligating the left coronary artery at the middle of anterior descending branch. The ECE and NOS gene expressions were observed by in situ hybridization and the cooper-zinc-SOD was studied by immunohistochemical and image analysis.


RESULTS
It was found that the transfer of ECE mRNA was greatly increased, while that of NOS mRNA was greatly decreased in ischemic my ocardium, but the YXT could enhance the expression levels of NOS mRNA and inhibit the over expression of ECE mRNA in ischemic myocardium in dog. Meanwhile, the cooper-zinc-SOD immunohistochemistry was significantly weakened in ischemic myocardium, but the YXT could evidently increase SOD activity in experimental ischemic myocardium.


CONCLUSIONS
It suggested that YXT had notable protection on experimental ischemic myocardium and was definitely valuable on prevention and treatment of coronary heart disease.
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000072396:Endothelin-Converting Enzymes; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism*