[Parameters of hemolysis by sodium dodecyl sulfate as an indicator of the structural states of erythrocyte membranes].
The relationship between the parameters (percentage and rate) of hemolysis by sodium dodecyl sulfate and the time of incubation of human erythrocyte suspensions in glucose-free medium at 37 degrees C was studied. The polyphasic changes in the parameters were found, which depend on the mode of pretreatment: ATP depletion by iodoacetate, heat denaturation of spectrin, and treatment of cells by valinomycin. It was found that the percentage and rate of detergent hemolysis do not always change in parallel.
D000255:Adenosine Triphosphate / Q000378:metabolism; D003470:Culture Media; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013049:Spectrin / Q000737:chemistry; D014634:Valinomycin / Q000494:pharmacology