title: Maternal mRNA localization of zebrafish DAZ-like gene.
abstract: Members of the DAZ gene family encode RNA-binding proteins and have been shown to play a pivotal role in gametogenesis. In Xenopus, a DAZ-like gene encodes an RNA component of the germ plasm. We have identified a zebrafish DAZ homologue, zDazl. zDazl mRNA was expressed in gonads of both sexes. In ovary, it was localized in the cortex of oocytes. At the onset of embryogenesis, maternal zDazl mRNA was detected at the vegetal pole. It migrated toward blastomeres through cytoplasmic streams as early embryogenesis proceeded. This is the first report showing maternal mRNA localization at the vegetal pole in fish and the existence of mRNA streams in the yolk cytoplasm.
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D000075364:Deleted in Azoospermia 1 Protein; D005260:Female; D015723:Gene Library; D017403:In Situ Hybridization; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D015027:Zebrafish / Q000196:embryology*