[Preparation of NADPH-cytochrome P-450 reductase and the role in metabolism of xenobiotics].
NADPH-Cytochrome P-450 Reductase is the major part of MFO (mixed function oxidase) system of liver. A simple and economic method of preparation of reductase was introduced in this paper and it was certified that xenobiotics benzene, bichloroethylene, and asbestos can be oxidized by reductase and reconstituted cytochrome P-450 2B1. But the metabolites of the 3 compounds can not cause the changes of plasmid PBR322 DNA.
D000818:Animals; D001194:Asbestos / Q000378:metabolism; D001554:Benzene / Q000378:metabolism; D005025:Ethylene Dichlorides / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015262:Xenobiotics / Q000378:metabolism*