Monoclonal antibodies against integrin subunits alpha6 and beta1 inhibit migration of gingival epithelium in organ culture.
BACKGROUND
The main problem in the posterior instrumental treatment of periodontitis is the re-epithelization of the periodontal defects, leading to the formation of an unphysiological, long junctional epithelium inhibiting the regeneration of periodontal attachment. A precondition for the re-epithelization is the interaction between epithelial cells and their extracellular matrix mediated by integrins. Integrins are cellular adhesion molecules (CAM), binding to different structures of the extracellular matrix, e.g., collagen, laminin, or fibronectin.


METHODS
Biopsy specimens of marginal gingiva, composed of epithelium and subepithelial connective tissue, were cultivated on microporous membranes in tissue culture plates. The culture medium was supplemented with monoclonal antibodies directed against human integrin subunits. The period of cultivation was 6 days and the medium was replaced daily. After cultivation, the tissue development was analyzed by histological and immunohistochemical methods.


RESULTS
We found that the combination of antibodies directed against the integrin subunits alpha6 and beta1 inhibited the migration of epithelium completely in 9 out of 10 cultures, whereas control cultures containing none or only irrelevant antibodies demonstrated connective tissues completely covered by epithelium.


CONCLUSIONS
Influencing the regeneration of periodontal tissue on a molecular basis by using antibodies directed against integrin subunits may be of future interest in postsurgical treatment of periodontal diseases.
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement; D003239:Connective Tissue Cells / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology; D005881:Gingiva / Q000166:cytology* / Q000502:physiology; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D039121:Integrin alpha6beta1; D016023:Integrins / Q000037:antagonists & inhibitors* / Q000502:physiology; D009924:Organ Culture Techniques; D010513:Periodontal Ligament / Q000502:physiology