[Comparison of partial sequencing of 5' non-coding region of seven HGV strains isolated from different areas in China].
OBJECTIVE
To explore the variation of Chinese hepatitis G virus (HGV) strains.


METHODS
HGV RNA was detected by reverse transcription nested polymerase chain reaction (RT-nPCR) with a designed primer derived from the 5' non-coding region of Chinese HGV strains. HGV cDNA was sequenced in seven samples with positive products by ABI prism 377 DNA automatic sequencer.


RESULTS
Homology of nucleotide sequence of the 5' non-coding region of HGV strains in seven Chinese strains was 88.26%, 85.92%, 88.26%, 85.45%, 86.85%, 85.92% and 88.26%, respectively, as compared with the African strains GBV-C (u 36380), and 92.02%, 86.85%, 86.67%, 89.02%, 89.67%, 89.67% and 91.55%, respectively, as compared with the strains isolated from the U.S. (u 44402). Homology of nucleotide sequence was 92.02% to 97.18% between HGV strains isolated in China.


CONCLUSIONS
Genotypes of Chinese HGV strains differ from those of GBV-C and HGV subtypes reported in the U.S.
D020121:5' Untranslated Regions; D001483:Base Sequence; D018067:Flaviviridae / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid