A comparison of 15N proline and 13C leucine for monitoring protein biosynthesis in the skin.
The tracers L 15N-proline and L(1-13C)-leucine were used to explore the synthesis of skin proteins in vivo in rabbits. They orally received a single dose containing an equimolecular mixture of L(1-13C)-leucine and L 15N-proline. The changes in the amounts of these tracers in blood and skin were monitored for a total of 8 h. The data showed the appearance of the two tracers in blood within 15 min and their clearance in 8 h. They were both rapidly (15 min) incorporated into skin proteins, but more proline was incorporated than leucine. We therefore consider L 15N-proline to be a better tracer than L(1-13C)-leucine for studying protein metabolism in the skin.
D000818:Animals; D002247:Carbon Isotopes; D007930:Leucine / Q000097:blood / Q000378:metabolism*; D009587:Nitrogen Isotopes; D011392:Proline / Q000097:blood / Q000378:metabolism*; D014176:Protein Biosynthesis; D011817:Rabbits; D011849:Radioactive Tracers; D012867:Skin / Q000378:metabolism*; D013997:Time Factors