Increase in apoptosis and concomitant reduction of ischemic lesion volume and evidence for synaptogenesis after transient focal cerebral ischemia in rat treated with staurosporine.
We sought to determine whether induction of apoptosis alters an ischemic lesion. Rats were subjected to 2 h of middle cerebral artery occlusion (MCAo) and treated with staurosporine (n=8) or vehicle (n=4). Our data demonstrate that at 22 h after MCAo, staurosporine triggered a significant increase in apoptosis, a reduction of lesion volume and an increase of synaptophysin immunoreactivity, as compared to the control group.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001157:Arterial Occlusive Diseases / Q000188:drug therapy / Q000473:pathology; D001921:Brain / Q000098:blood supply / Q000201:enzymology / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000473:pathology*; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D019311:Staurosporine / Q000494:pharmacology*; D013569:Synapses / Q000473:pathology*