title: Multiple cadherin mRNA expression and developmental regulation of a novel cadherin in the developing mouse eye.
abstract: Cadherins form a large family of transmembrane glycoproteins whose members include the classical cadherins, the desmosomal cadherins, and the protocadherins. The classical cadherins mediate homophilic cell-cell adhesion and are key regulators of many morphogenetic processes. More than a dozen classical cadherins are expressed in both the developing and the mature central nervous system. Although individual cadherins have been identified in the retina of various species, we wished to determine the range of cadherins expressed at distinct developmental stages in the mouse retina. Using a PCR-based cloning strategy, we detected 10 different classical cadherin mRNAs of both type I and type II subtypes. The most abundant cDNA was that encoding the type II cadherin, Cadherin-11. The other type II cadherins detected were VE- and T2-cadherin and Cadherin-6 and -12. Four type I cadherins, N-, R-, P-, and E-cadherin, were also present. One cadherin cDNA encoded a novel cadherin, called EY-cadherin for cloned from eye. EY-cadherin is most closely related to human Cadherin-14 (93% identical). EY-cadherin mRNA was detected in the adult mouse eye, brain, and testis with a 20-fold increase in expression levels in the embryonic head from E11 to E19 and a 50-fold increase in expression levels in the postnatal eye from PN1 to PN16. Multiple cadherin gene expression is consistent with the hypothesis that different cadherins regulate morphogenetic processes, such as neuronal migration and lamination, and determine the specific interneuronal connections found in the mature retina.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D002448:Cell Adhesion; D018076:DNA, Complementary / Q000235:genetics; D005123:Eye / Q000196:embryology* / Q000254:growth & development; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity