Periplakin and envoplakin are target antigens in canine and human paraneoplastic pemphigus.
BACKGROUND
On the basis of clinical and histopathologic similarities to human paraneoplastic pemphigus (PNP), we recently identified the first case of PNP in a nonhuman species, the dog.


OBJECTIVE
To determine a similar pathogenesis in both species, the present study aimed to define whether common antigens are targeted in dog and man.


METHODS
Canine and human PNP sera were used in parallel to immunoprecipitate 14C-labeled human keratinocyte antigens. The immunoreactive proteins were then identified by immunoprecipitation of canine keratinocyte extracts with specific antibodies to the antiplakin family members follwed by immunoblot analysis using canine and human PNP sera.


RESULTS
Protein bands of 210, 190, 170, and 130 kd were identified in dogs and humans. In both species, envoplakin and periplakin were demonstrated as antigens. Anti-desmoglein 3 antibodies could not be demonstrated in canine PNP, but in human PNP.


CONCLUSIONS
These results demonstrate that canine PNP closely correlates to the human counterpart and may therefore represent an excellent model for the human disease.
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis; D015820:Cadherins / Q000276:immunology; D002250:Carbon Radioisotopes; D003598:Cytoskeletal Proteins / Q000276:immunology*; D051184:Desmoglein 3; D003896:Desmosomes / Q000276:immunology*; D004195:Disease Models, Animal; D004285:Dogs; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D008970:Molecular Weight; D010257:Paraneoplastic Syndromes / Q000276:immunology*; D010392:Pemphigus / Q000276:immunology*; D051179:Plakins; D011233:Precipitin Tests; D011498:Protein Precursors / Q000276:immunology*; D019275:Radiopharmaceuticals