Effect of ion modification of commonly used orthopedic materials on the attachment of human bone-derived cells.
Biomaterials which combine optimum properties of strength and biocompatibility are desirable in improving the long-term performance of implantable medical devices. Our study is aimed at developing technology designed to alter the outer atomic layers of a material to give the desired compatibility with the tissue while retaining the properties of the bulk substratum. Materials used in this study were titanium vanadium alloy (Ti-6Al-4V) and cobalt chromium molybdenum alloy (Co-Cr). Soda lime glass discs and polyethylene terephthalate (PET) acted as controls. A cathode of either Ti-6Al-4V or Co-Cr was used to simultaneously deposit and implant identified substrata. The attachment of human bone-derived cells (HBDC) to various materials was determined using radiolabeling or colorimetric assays. Results show that HBDC adhere preferentially to the unmodified surfaces of Ti-6Al-4V and Ti-6Al-4V on glass compared to the unmodified Co-Cr surfaces and to that of the Co-Cr on glass. Depositing Ti-6Al-4V on Co-Cr gives significantly better attachment of HBDC than when depositing Co-Cr onto Ti-6Al-4V. While cellular attachment to the created surfaces reflects that of the cathodic materials, it is not identical to these materials. Ion deposition/implantation is capable of creating permanent surfaces which reflect the adhesion of source materials not bulk substrata.
D000497:Alloys; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology*; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002858:Chromium Alloys / Q000737:chemistry / Q000494:pharmacology; D003124:Colorimetry; D004577:Electron Probe Microanalysis; D005898:Glass; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D013778:Tetrazolium Salts; D013844:Thiazoles; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology; D014961:X-Ray Diffraction