Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours.
The RCK gene is a target of the t(11;14)(q23;q32) chromosomal translocation observed in human B-cell lymphoma, and the overexpression of its protein (rck/p54) by the translocation was shown to cause malignant transformation. The rck/p54 protein belongs to the DEAD box protein/RNA helicase family, which has a variety of functions such as translation initiation, pre-mRNA splicing and ribosome assembly. The expression of rck p54 in colorectal adenocarcinoma cells was examined by immunohistochemistry and Western blot analysis. The rck/p54 protein was found to be overexpressed in tumour tissues resected from 13 (50%) out of 26 cases of colorectal adenocarcinomas and two out of two (100%) cases of colonic severe dysplastic adenomas. In view of activities of rck/p54 determined in other tissue types, we suggest that rck/p54 may contribute to the cell proliferation and carcinogenesis at the translational level in the development of colorectal tumours.
Nakagawa
Y
Y
The Second Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan.
Morikawa
H
H
Hirata
I
I
Shiozaki
M
M
Matsumoto
A
A
Maemura
K
K
Nishikawa
T
T
Niki
M
M
Tanigawa
N
N
Ikegami
M
M
Katsu
K
K
Akao
Y
Y
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D053487:DEAD-box RNA Helicases; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000235:genetics
D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D012316:RNA Nucleotidyltransferases; C094683:DDX6 protein, human; D053487:DEAD-box RNA Helicases