[The effect of coenzyme Q10 on lactate acidosis at the beginning of experimental cerebral ischemia in rats after the use of endothelin 1 (preliminary results)].
The aim of the work was to evaluate effect of CoQ10 on the lactate level in the rat brain after administration of Et-1. Fifty-three male Wistar rats (approximately 220-250 g) were used in the experiment. The animals were divided into four groups. 1. control; 2-4 under chloride hydrate anaesthesia, the rats were subjected to stereotaxic injection into right cerebral lateral ventricle: 2. 1 microliter Ringer's solution (control) and i.p. 0.5 ml of soybean oil, 3. 10 pmoles Et-1 diluted in 1 microliter Ringer's solution and i.p. 0.5 ml soybean oil, 4. 10 pmoles Et-1 diluted in 1 microliter Ringer's solution and i.p. 10 mg/kg of the body weight CoQ1O (Sigma). Right and left hemispheres were taken after one hour, 4 and 24 hours and the lactate level was assayed by an enzymatic method. Et-1 evoked a significant increase of lactate level at all experimental time intervals. CoQ10 reduced lactate acidosis after 24 hours from the administration of Et-1. The above phenomenon can be explained among other by an improvement of the cellular respiratory activity. CoQ10 represents very important electron transporter from flavins to cytochromes in the respiratory chain. Therefore CoQ10 can be used for prevention and treatment of ischaemic insult in human cases.
Piotrowski
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P
Pracowni Neurochemii Klinicznej i Zakładu Neuropatologii, Instytutu Centrum Medycyny Doświadczalnej i Klinicznej P.A.N. w Warszawie.
Ostrowski
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RP
Pańkowska
T
T
Smiałek
M
M
D000140:Acidosis, Lactic / Q000188:drug therapy* / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000098:blood supply; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism*; D019332:Endothelin-1 / Q000187:drug effects* / Q000378:metabolism*; D019344:Lactic Acid / Q000032:analysis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014451:Ubiquinone / Q000494:pharmacology* / Q000627:therapeutic use*
D019332:Endothelin-1; D014451:Ubiquinone; D019344:Lactic Acid