Effect of diclofenac sodium and dexamethasone on cultured human Tenon's capsule fibroblasts.
OBJECTIVE
To investigate the effect of diclofenac sodium and dexamethasone on cultured human Tenon's capsule fibroblasts.


METHODS
Two experiments were conducted. In the first experiment, fibroblasts were treated with either diclofenac sodium or dexamethasone at different concentrations, and the cell growth was quantified by using Coulter counter and hexosaminidase methods at 1, 3, 5, and 7 days after adding the drugs. In the second experiment, the cells were treated with each drug for 24 hours and then the cultures were switched to a drug-free medium. The cell growth was quantified at day 7 after removing the drugs from the medium.


RESULTS
In the first experiment, inhibition of fibroblast growth in a dose-dependent manner was observed from days 1 to 7 in the cultures treated with each drug. The inhibitory was more pronounced in the diclofenac treated cultures. The typical spindle-shaped fibroblasts treated with higher concentrations of the drugs became spherical cells. In the second experiment, inhibition was not observed when the cultures were switched to a drug-free medium. The spherical cells recovered to spindle-shaped cells and proliferated as normal cells.


CONCLUSIONS
Our results have shown that diclofenac sodium and dexamethasone can significantly inhibit human Tenon's capsule fibroblast growth in a cell culture model. The inhibitory effect was not observed when the cultures were switched after 24 hours to a drug-free culture medium.
Sun
R
R
Gimbel Eye Centre, Calgary, Alberta, Canada.
Gimbel
H V
HV
Liu
S
S
Guo
D
D
Hollenberg
M D
MD
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003239:Connective Tissue Cells / Q000187:drug effects* / Q000201:enzymology; D003907:Dexamethasone / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005938:Glucocorticoids; D004008:Diclofenac; D003907:Dexamethasone; D001619:beta-N-Acetylhexosaminidases