High-performance liquid chromatographic assay of (+/-)-lactic acid and its enantiomers in calf serum.
Two high-performance liquid chromatographic methods are described for the determination of lactic acid and its enantiomers in calf serum. A 300x8.0 mm I.D. column packed with sulfonated styrene-divinylbenzene copolymer and a 50x4.6 mm ODS column with N,N-dioctyl-L-alanine were used. UV detection was at 205 and 236 nm for the non-chiral and chiral assays, respectively. Both assays demonstrated excellent linear relationships between peak area ratios and serum concentrations over a range of 0.5 to 20 mM, based on 100 microl bovine serum. Recovery was complete. Inter- and within-batch bias and relative standard deviation were <15%.
Omole
O O
OO
College of Pharmacy and Nutrition and The Department of Internal Medicine, University of Saskatchewan, Saskatoon, Canada.
Brocks
D R
DR
Nappert
G
G
Naylor
J M
JM
Zello
G A
GA
D000818:Animals; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019344:Lactic Acid / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D019344:Lactic Acid