title: Disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of Vibrio parahaemolyticus or Vibrio cholerae El Tor hemolysin.
abstract: It is believed that the thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus and El Tor hemolysin (ETH) of Vibrio cholerae damage erythrocytes and other cells by acting as pore-forming toxins. In this study, we found that a protein band with a molecular weight of 37,000 daltons specifically disappeared from erythrocyte membrane after hemolysis by TDH and ETH, but not after hypotonic hemolysis. The 37 kDa band was identified as glyceraldehyde 3-phosphate dehydrogenase (G3PD), a glycolytic enzyme, based on N-terminal 14 amino acid sequencing. The role of G3PD in hemolysis is discussed.
authors.affiliation: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan. chang@biken.osaka-u.ac.jp
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Honda
mesh_terms: D001426:Bacterial Proteins; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D014734:Vibrio cholerae / Q000378:metabolism*; D014736:Vibrio parahaemolyticus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases