title: Functional analysis of poly(ADP-ribose) polymerase in Drosophila melanogaster.
abstract: Poly(ADP-ribose) polymerase (PARP) is conserved in eukaryotes. To analyze the function of PARP, we isolated and characterized the gene for PARP in Drosophila melanogaster. The PARP gene consisted of six translatable exons and spanned more than 50 kb. The DNA binding domain is encoded by exons 1-4. Although the consensus cleavage site of CED-3 like protease during apoptosis is conserved from human to Xenopus laevis PARPs, it is neither conserved in the corresponding region of Drosophila nor Sarcophaga peregrina. There are two cDNAs species in Drosophila. One cDNA could encode the full length PARP protein (PARP I), while the other is a truncated cDNA which could encode a partial-length PARP protein (PARP II), which lacks the automodification domain and is possibly produced by alternative splicing. The expression of these two forms of PARP in E. coli demonstrated that while PARP II has the catalytic NAD-binding domain and DNA-binding domain it is enzymatically inactive. On the other hand PARP I is active. A deletion mutant of PARP gene could grow to the end of embryogenesis but did not grow to the adult fly. These results suggest that the PARP gene plays an important function during the development of Drosophila.
authors.affiliation: Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miwa
authors.forename: S
authors.initials: S
authors.lastname: Hanai
authors.forename: P
authors.initials: P
authors.lastname: Poltronieri
authors.forename: M
authors.initials: M
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Uchida
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics*; D005799:Genes, Dominant; D008954:Models, Biological; D008957:Models, Genetic; D016296:Mutagenesis; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D011065:Poly(ADP-ribose) Polymerases