sgk is an aldosterone-induced kinase in the renal collecting duct. Effects on epithelial na+ channels.
The early phase of the stimulatory effect of aldosterone on sodium reabsorption in renal epithelia is thought to involve activation of apical sodium channels. However, the genes initiating this effect are unknown. We used a combination of polymerase chain reaction-based subtractive hybridization and differential display techniques to identify aldosterone-regulated immediate early genes in renal mineralocorticoid target cells. We report here that aldosterone rapidly increases mRNA levels of a putative Ser/Thr kinase, sgk (or serum- and glucocorticoid-regulated kinase), in its native target cells, i.e. in cortical collecting duct cells. The effect occurs within 30 min of the addition of aldosterone, is mediated through mineralocorticoid receptors, and does not require de novo protein synthesis. The full-length sequences of rabbit and mouse sgk cDNAs were determined. Both cDNAs show significant homology to rat and human sgk (88-94% at the nucleotide level, and 96-99% at the amino acid level). Coexpression of the mouse sgk in Xenopus oocytes with the three subunits of the epithelial Na+ channel results in a significantly enhanced Na+ current. These results suggest that sgk is an immediate early aldosterone-induced gene, and this protein kinase plays an important role in the early phase of aldosterone-stimulated Na+ transport.
Náray-Fejes-Tóth
A
A
Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756-0001, USA. aniko.fejes-toth@dartmouth.edu
Canessa
C
C
Cleaveland
E S
ES
Aldrich
G
G
Fejes-Tóth
G
G
D000450:Aldosterone / Q000096:biosynthesis / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000595:Amino Acid Sequence; D000732:Androstanols / Q000494:pharmacology; D000818:Animals; D004553:Electric Conductivity; D004790:Enzyme Induction; D017874:Immediate-Early Proteins; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000201:enzymology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D018161:Receptors, Mineralocorticoid / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D000099184:Serum-Glucocorticoid Regulated Kinases
D000450:Aldosterone; D000584:Amiloride; D000732:Androstanols; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D018161:Receptors, Mineralocorticoid; D011994:Recombinant Proteins; D000099184:Serum-Glucocorticoid Regulated Kinases; C049154:11,17-dihydroxy-6-methyl-17-(1-propynyl)androsta-1,4,6-triene-3-one