title: Identification of genes directly and indirectly involved in the insect transmission of African cassava mosaic geminivirus by Bemisia tabaci.
abstract: The inability to transmit progeny virus resulting from the cloned components of an isolate of African cassava mosaic virus originating from Kenya (ACMV-K) has been shown to be due to defects in both genomic components. This was achieved by the production of infectious pseudorecombinants between ACMV-K and the cloned components of a whitefly-transmissible ACMV isolate originating from Nigeria (ACMV-NOg). The exchange of gene fragments between ACMV-K and ACMV-NOg has been used to demonstrate that the defects responsible for lack of transmissibility reside on the coat protein and DNA B C1 gene of ACMV-K. The significance of these finding with respect to the present understanding of the function of these gene products are discussed.
authors.affiliation: Department of Virus Research, John Innes Centre, Norwich, UK.
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: R W
authors.initials: RW
authors.lastname: Briddon
authors.forename: I D
authors.initials: ID
authors.lastname: Bedford
authors.forename: M S
authors.initials: MS
authors.lastname: Pinner
authors.forename: P G
authors.initials: PG
authors.lastname: Markham
mesh_terms: D000818:Animals; D002213:Capsid / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D005814:Genes, Viral; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D002365:Manihot / Q000821:virology*; D010935:Plant Diseases; D018515:Plant Leaves / Q000821:virology; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins