Localization of GTPase-activating protein-(GAP) like immunoreactivity in mouse cerebral regions.
GTPase-activating protein is known to regulate the conversion between ras-GTP and ras-GDP. We studied the basal expression of GTPase-activating protein-like immunoreactivity in mouse cerebral regions using a polyclonal anti-GTPase-activating protein antibody. Cells with GTPase-activating protein-like immunoreactivity were distributed in frontal cortical layers IV and V, and in the parietal cortex, piriform cortex, amygdaloid area, septum, lateral thalamus, and hypothalamus. The GTPase-activating protein-like immunoreactivity was also observed in fiber-like structures in the caudate putamen, stria terminalis, internal capsule, and medial forebrain bundle, and around CA2 pyramidal cells in Ammon's horn. These results imply that GTPase-activating protein is constitutively expressed in mouse brain regions and may have physiological functions in specific neuronal pathways in the brain.
Namima
M
M
Center for Laboratory Animal Science, National Defense Medical College, Saitama, Japan.
Takeuchi
K
K
Watanabe
Y
Y
Yamano
M
M
Saito
M
M
Sasa
H
H
Okamoto
K
K
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D002552:Cerebral Ventricles / Q000378:metabolism; D003337:Corpus Callosum / Q000378:metabolism; D005625:Frontal Lobe / Q000378:metabolism; D020690:GTPase-Activating Proteins; D006624:Hippocampus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009928:Organ Specificity; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D011699:Putamen / Q000378:metabolism; D017966:Pyramidal Cells / Q000166:cytology / Q000378:metabolism; D012686:Septal Nuclei / Q000378:metabolism; D020703:ras GTPase-Activating Proteins
D020690:GTPase-Activating Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins