Identification and characterization of excreted-secreted products and surface coat antigens of animal and plant-parasitic nematodes.
Nematode surface coat (SC) proteins and excreted-secreted products (E-S) are likely to play important roles in the host-parasite interaction and considerable similarities can be found in SC proteins and E-S products from certain plant and animal parasitic nematodes. Monoclonal antibodies raised to E-S products of plant-parasitic nematodes were shown to cross-react with E-S products and the surface coats of the animal parasites Trichinella spiralis and Haemonchus contortus. Most of the antibodies recognized carbohydrate epitopes but the activity of 2 MAbs (IACR-CCNj.2a.15 and IACR-Misec.8D.3) which recognized proteic epitopes in these nematodes were further characterized. Antibody 2a.15 recognized the SC and oral exudate of Meloidogyne incognita, T. spiralis and H. contortus. This antigen was also immunolocalized in the lining of the oesophagus and gut and in the exudate present during ecdysis of H. contortus L3. Antibody 8D.3 reacted with the SC of these nematodes on cryosections but on live nematodes the immunofluorescence was very patchy and was shed from the nematode SC.
Lopez de Mendoza
M E
ME
Entomology and Nematology Department, IACR-Rothamsted, Herts, UK.
Curtis
R H
RH
Gowen
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006189:Haemonchus / Q000276:immunology / Q000378:metabolism; D015801:Helminth Proteins / Q000276:immunology*; D010935:Plant Diseases / Q000469:parasitology*; D017160:Trichinella spiralis / Q000276:immunology / Q000378:metabolism; D014431:Tylenchoidea / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D015801:Helminth Proteins