Effective inhibition of Candida albicans growth by the combination of murine peritoneal neutrophils and activated macrophages.
The effect of leukocytes on the anti-Candida activity of neutrophils was examined. Murine neutrophils which were purified from casein-induced peritoneal cells inhibited the mycelial growth of Candida albicans. This anti-Candida activity of neutrophils was augmented by the addition of spleen cells prepared from mice pretreated with bacterial lipopolysaccharide 3 hr before, but not from non-treated mice. The population in the spleen cells, which enhanced the anti-Candida activity of neutrophils, was plastic-plate adherent, nylon-fiber columns adherent and anti-Mac-1 antigen-positive. These immunological profiles suggested that the enhancing cells are classified to splenic macrophages. Peritoneal-exudated macrophages from mice treated with lipopolysaccharide also augmented the anti-Candida activity of neutrophils. These results suggest that the anti-Candida activity of neutrophils may be upregulated by activated macrophages.
Tansho
S
S
Department of Microbiology and Immunology, University of Teikyo School of Medicine, Tokyo, Japan.
Abe
S
S
Tansho
T
T
Yamaguchi
H
H
D000818:Animals; D002176:Candida albicans / Q000254:growth & development* / Q000276:immunology*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D008070:Lipopolysaccharides