Interactions between 192-IgG saporin and intraseptal cholinergic and GABAergic drugs: role of cholinergic medial septal neurons in spatial working memory.
Rats were administered 192-IgG saporin (SAP) or vehicle into the medial septum-vertical limb of the diagonal band (MS-vDB). Starting 1 week later, the effects of intraseptal scopolamine, oxotremorine, and muscimol were tested in a T-maze alternation task. Choice accuracy in the absence of infusions did not differ between control and SAP-treated rats. Intraseptal scopolamine or muscimol impaired the choice accuracy of SAP-treated but not control rats. Oxotremorine impaired accuracy similarly in control and SAP-treated rats. The enhanced effects of scopolamine and muscimol produced by SAP are consistent with the hypothesis that cholinergic MS-vDB neurons are used in spatial working memory. The finding that SAP alone did not alter choice accuracy provides further evidence that cholinergic MS-vDB neurons are not necessary for spatial working memory. Thus, cholinergic MS-vDB neurons are involved in but not necessary for spatial working memory.
Pang
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KC
Department of Psychology, Bowling Green State University, Ohio 43403, USA. kpang@bgnet.bgsu.edu
Nocera
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D018678:Cholinergic Agents / Q000494:pharmacology*; D004347:Drug Interactions; D005625:Frontal Lobe / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D018682:GABA Agents / Q000494:pharmacology*; D000922:Immunotoxins / Q000494:pharmacology*; D007276:Injections, Intraventricular; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D008568:Memory / Q000187:drug effects* / Q000502:physiology*; D009118:Muscimol / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases; D009475:Neurons, Afferent / Q000187:drug effects; D010095:Oxotremorine / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D012601:Scopolamine / Q000494:pharmacology; D013028:Space Perception / Q000187:drug effects / Q000502:physiology
C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D018678:Cholinergic Agents; D018682:GABA Agents; D000922:Immunotoxins; D054789:Ribosome Inactivating Proteins, Type 1; D009118:Muscimol; D010095:Oxotremorine; D012601:Scopolamine; D009699:N-Glycosyl Hydrolases; D000076985:Saporins