Glucocorticoids inhibit proliferation and adhesion of the IL-3-dependent mast cell line, MC/9, to NIH/3T3 fibroblasts, with an accompanying decrease in IL-3 receptor expression.
We investigated the effects of glucocorticoids on IL-3-dependent proliferation and c-kit expression of cells of the mouse mast cell line, MC/9. Glucocorticoids (dexamethasone, prednisolone, and hydrocortisone) inhibited IL-3-dependent MC/9 cell proliferation, whereas sex steroids (progesterone, beta-estradiol, and testosterone) had no effect. Flow cytometric analysis revealed that glucocorticoids reduced the expression of the IL-3 receptor on MC/9 cells. Immunoblot and Northern blot analyses indicated that glucocorticoids also reduced the expression of both c-kit protein and c-kit mRNA transcript. Furthermore, the adhesion of MC/9 cells to stem cell factor-expressing NIH/3T3 cells was reduced following glucocorticoid treatment. Our results indicate that glucocorticoids inhibit IL-3-dependent MC/9 mast cell proliferation, with an accompanying decrease in IL-3 receptor expression. Glucocorticoids also reduced c-kit expression on MC/9 cells resulting in a decreased adhesion to NIH/3T3 fibroblasts.
Sakai
H
H
Department of Dermatology, Asahikawa Medical College, Hokkaido, Japan.
Toyota
N
N
Ito
F
F
Takahashi
H
H
Hashimoto
Y
Y
Iizuka
H
H
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005434:Flow Cytometry; D016195:G2 Phase; D015870:Gene Expression / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007377:Interleukin-3 / Q000502:physiology*; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008938:Mitosis; D011239:Prednisolone / Q000494:pharmacology; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000096:biosynthesis / Q000187:drug effects*; D016196:S Phase; D013739:Testosterone / Q000494:pharmacology
D005938:Glucocorticoids; D007377:Interleukin-3; D012333:RNA, Messenger; D016185:Receptors, Interleukin-3; D013739:Testosterone; D004958:Estradiol; D003907:Dexamethasone; D011239:Prednisolone; D019009:Proto-Oncogene Proteins c-kit; D006854:Hydrocortisone