Curative effect of picroliv on primary cultured rat hepatocytes against different hepatotoxins: an in vitro study.
Picroliv, the standardized active principle from the plant Picrorhiza kurrooa showed significant curative activity in vitro in primary cultured rat hepatocytes against toxicity induced by thioacetamide (200 microg/mL), galactosamine (400 microg/mL), and carbon tetrachloride (3 microl/mL). Activity was assessed by determining the change in hepatocyte viability and rate of oxygen uptake and other biochemical parameters (GOT, GPT, and AP). The toxic agents alone produced a 40-62% inhibition of cell viability and a reduction of biochemical parameters after 24 h of incubation at 37 degrees C which (on removal of the toxic agents) was reversed after further incubation for 48 h. Incubation of damaged hepatocytes with picroliv exhibited a concentration- (1-100 microg/mL) dependent curative effect in restoring altered viability parameters. The results warrant the use of this in vitro system as an alternative for in vivo assessment of hepatoprotective activity of new agents.
Visen
P K
PK
Division of Pharmacology, Central Drug Research Institute, Lucknow, UP, India.
Saraswat
B
B
Dhawan
B N
BN
D000818:Animals; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005688:Galactosamine / Q000037:antagonists & inhibitors / Q000633:toxicity; D006027:Glycosides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013853:Thioacetamide / Q000037:antagonists & inhibitors / Q000633:toxicity; D014641:Vanillic Acid / Q000494:pharmacology*
D002934:Cinnamates; D006027:Glycosides; D010936:Plant Extracts; D013853:Thioacetamide; C057463:kutkin; D005688:Galactosamine; D002251:Carbon Tetrachloride; D014641:Vanillic Acid