Comparison between automatic ribotyping and random amplified polymorphic DNA analysis of Bacillus cereus isolates from the dairy industry.
Discrimination by automatic ribotyping and random amplified polymorphic DNA PCR, RAPD, was compared for 40 different B. cereus dairy isolates, 4 different B. mycoides isolates and 6 culture collection strains. RAPD-PCR has previously shown to be useful for tracing contamination routes of B. cereus to milk. Automatic ribotyping using EcoRI and PvuII separated the B. cereus and B. mycoides isolates/strains into 36 different ribotypes. RAPD-typing with primers generated 40 different RAPD-profiles. However, 17 isolates clustered into eight groups, irrespective of the primer and restriction enzyme used, and in all but one case, the isolates with the same pattern were isolated from the same dairy. Automatic ribotyping proved to be a useful, standardized and quick method to discriminate between B. cereus strains, only slightly less discriminatory than RAPD-typing.
Andersson
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A
SIK, The Swedish Institute for Food and Biotechnology, Göteborg. annika.andersson@sik.se
Svensson
B
B
Christiansson
A
A
Rönner
U
U
D000818:Animals; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry*; D003612:Dairying; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D005516:Food Microbiology; D005525:Food-Processing Industry; D005517:Foodborne Diseases / Q000517:prevention & control; D008858:Microscopy, Phase-Contrast; D009716:Numerical Analysis, Computer-Assisted; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015246:Deoxyribonuclease EcoRI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific