Ligand recognition in multiallelic pheromone receptors from the basidiomycete Schizophyllum commune studied in yeast.
The homobasidiomycete Schizophyllum commune encodes a multiallelic pheromone receptor system that distinguishes more than 20 nonself from at least 2 self pheromones. The well-investigated pheromone response system of the yeast Saccharomyces cerevisiae was used to link the FUS1::lacZ reporter system to the heterologous pheromone receptors from S. commune. To investigate yeast G-protein binding, the unchanged heterologous receptor was compared to constructs carrying an exchange of the 3rd cytoplasmatic loop for the Ste2 sequence. A better coupling could be achieved with the altered constructs. In order to examine activation by single pheromones, an artificial peptide based on the sequence of a new putative pheromone gene, bap2(1), in the Balpha2 mating-type locus encoding the shortest pheromone found so far in fungal mating types was used. Thus, we have reassembled the pheromone recognition of the basidiomycete S. commune and constructed a system ideal for specificity analysis in the yeast S. cerevisiae.
Hegner
J
J
Department of Biology-Genetics, Philipps University, Marburg, 35032, Germany.
Siebert-Bartholmei
C
C
Kothe
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D002628:Chemoreceptor Cells / Q000378:metabolism*; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D017930:Genes, Reporter / Q000235:genetics; D008024:Ligands; D008969:Molecular Sequence Data; D012567:Schizophyllum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D008024:Ligands