title: Tissue response to potential root-end filling materials in infected root canals.
abstract: The tissue responses to two potential root-end filling materials, a light-cured glass ionomer cement (Vitrebond) and a reinforced zinc oxide-eugenol cement (Kalzinol) were compared with that to amalgam. In 27 premolar teeth of beagle dogs (54 roots), a collection of endodontic pathogenic bacteria was first inoculated into the root canals to induce periapical lesions. On each root, an apicectomy was performed and root-end cavities prepared to receive fillings of each material. The teeth and surrounding jaw were removed after 8 weeks (24 roots) and 4 weeks (30 roots); and they were prepared for histological examination. The tissue response to amalgam fillings after 4 and 8 weeks was marked by moderate or severe inflammation on all roots, and extended > 0.5 mm in 10 out of 18 roots. In contrast, after 8 weeks, the majority of roots filled with Kalzinol showed little or moderate inflammation while the tissue response to Vitrebond was the best of the three materials, and was also less extensive. After 4 weeks, the overall best tissue response was with Kalzinol, followed closely by Vitrebond. The differences between materials for both time periods with either none or few inflammatory cells when compared with that with either moderate or severe inflammation were statistically significant (P < 0.01). Similarly, the differences between materials for both time periods with no inflammation or inflammation extending < 0.2 mm when compared with that with inflammation extending > 0.2 mm (< or = 0.5 mm or > 0.5 mm) were statistically significant (P < 0.01). Both Vitrebond and Kalzinol have potential as root-end filling materials as the tissue response was considerably more favourable than that to amalgam.
authors.affiliation: Department of Conservative Dentistry, United Medical and Dental Schools, Guy's Hospital, London, UK.
authors.forename: B S
authors.initials: BS
authors.lastname: Chong
authors.forename: T R
authors.initials: TR
authors.lastname: Ford
authors.forename: S P
authors.initials: SP
authors.lastname: Kariyawasam
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D003722:Dental Alloys / Q000633:toxicity; D003723:Dental Amalgam / Q000633:toxicity; D004285:Dogs; D004338:Drug Combinations; D005054:Eugenol / Q000633:toxicity; D005557:Formaldehyde / Q000633:toxicity; D005899:Glass Ionomer Cements / Q000633:toxicity; D010485:Periapical Periodontitis / Q000628:therapy; D010486:Periapical Tissue / Q000187:drug effects*; D012184:Retrograde Obturation / Q000379:methods*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D015034:Zinc Oxide / Q000633:toxicity
chemical_list: D003722:Dental Alloys; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D012387:Root Canal Filling Materials; C067790:Vitrabond; D005557:Formaldehyde; D005054:Eugenol; C036886:tytin; D003723:Dental Amalgam; C003799:N2 Dental Cement; D015034:Zinc Oxide