EXAFS study of zinc coordination in bacitracin A.
Bacitracin is a dodecapeptide antibiotic produced by Bacillus sp. The antibacterial activity depends upon the peptide binding a divalent metal. Hitherto, the exact coordination of the cation has not been established. In particular the role played by the sulphur and nitrogen atoms of the thiazoline ring of bacitracin A has not been clear. Here the coordination of Zn2+ by bacitracin A has been studied using extended X-ray absorption fine structure. The experimental data are consistent with a model in which zinc is coordinated by one oxygen and three nitrogen atoms with the sulphur atom of the thiazoline ring not being directly involved in the zinc coordination.
Drabløs
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SINTEF Unimed MR Centre, N-7465, Trondheim, Norway. finn.drablos@unimed.sintef.no
Nicholson
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DG
Rønning
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D000588:Amines / Q000737:chemistry; D001414:Bacitracin / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D018698:Glutamic Acid / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D008024:Ligands; D009584:Nitrogen; D010100:Oxygen; D013455:Sulfur; D013844:Thiazoles / Q000737:chemistry; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
D000588:Amines; D008024:Ligands; D013844:Thiazoles; D014867:Water; D001414:Bacitracin; D018698:Glutamic Acid; D006639:Histidine; D013455:Sulfur; C026461:bacitracin A; D015032:Zinc; D009584:Nitrogen; D010100:Oxygen