Neurons derived in vitro from ES cells express homeoproteins characteristic of motoneurons and interneurons.
We have characterized different neuronal subpopulations derived from in vitro differentiation of embryonic stem (ES) cells using as markers the expression of several homeodomain transcription factors. Following treatment of embryo-like aggregates with retinoic acid (RA), Pax-6, a protein expressed by ventral central nervous system (CNS) progenitors is induced. In contrast, Pax-7 expressed in vivo by dorsal CNS progenitors, and erbB3, a gene expressed by neural crest cells and its derivatives, are almost undetectable. CNS neuronal subpopulations generated expressed combinations of markers characteristic of somatic motoneurons (Islet-1/2, Lim-3, and HB-9), cranial motoneurons (Islet-1/2 and Phox2b) and interneurons (Lim-1/2 or EN1). Molecular characterization of neuron subtypes generated from ES cells should considerably facilitate the identification of new genes expressed by restricted neuronal cell lineages.
Renoncourt
Y
Y
INSERM U382, Developmental Biology Institute of Marseille (IBDM), CNRS/INSERM/Université de la Méditerranée/AP de Marseille, France. renoncou@ibdm.univ-mrs
Carroll
P
P
Filippi
P
P
Arce
V
V
Alonso
S
S
D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005136:Eye Proteins; D018507:Gene Expression Regulation, Developmental; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D060850:LIM-Homeodomain Proteins; D008562:Membrane Glycoproteins; D051379:Mice; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D000071841:PAX6 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; D064531:Peripherins; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D060850:LIM-Homeodomain Proteins; C094898:Lhx3 protein; D008562:Membrane Glycoproteins; D009124:Muscle Proteins; C109220:NBPhox protein; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D000071841:PAX6 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; C497322:Pax7 protein, mouse; D064531:Peripherins; D012097:Repressor Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; C084059:Hb9 protein, mouse; D014212:Tretinoin; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase