title: Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns.
abstract: Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein-tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in response to increased concentration of IP3. The detection of translocation enabled monitoring of IP3 concentration changes within single cells and revealed spatiotemporal dynamics in the concentration of IP3 synchronous with Ca2+ oscillations and intracellular and intercellular IP3 waves that accompanied Ca2+ waves. Such changes in IP3 concentration may be fundamental to Ca2+ signaling.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Tokyo and CREST, Japan Science and Technology Corporation, Tokyo 113-8654, Japan. hirose@calcium.cmp.m.u-tokyo.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: S
authors.initials: S
authors.lastname: Kadowaki
authors.forename: M
authors.initials: M
authors.lastname: Tanabe
authors.forename: H
authors.initials: H
authors.lastname: Takeshima
authors.forename: M
authors.initials: M
authors.lastname: Iino
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004285:Dogs; D049452:Green Fluorescent Proteins; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008164:Luminescent Proteins; D018613:Microscopy, Confocal; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D054803:Phospholipase C delta; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013997:Time Factors; D010738:Type C Phospholipases / Q000737:chemistry / Q000378:metabolism
chemical_list: D007295:Inositol Phosphates; D007527:Isoenzymes; D008024:Ligands; D008164:Luminescent Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D011993:Recombinant Fusion Proteins; C081655:inositol-1,3,4,5-tetrakisphosphate; D049452:Green Fluorescent Proteins; D015544:Inositol 1,4,5-Trisphosphate; D000255:Adenosine Triphosphate; D010738:Type C Phospholipases; D054803:Phospholipase C delta; D002118:Calcium