Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae.
The spindle checkpoint arrests cells in mitosis in response to defects in the assembly of the mitotic spindle or errors in chromosome alignment. We determined which spindle defects the checkpoint can detect by examining the interaction of mutations that compromise the checkpoint (mad1, mad2, and mad3) with those that damage various structural components of the spindle. Defects in microtubule polymerization, spindle pole body duplication, microtubule motors, and kinetochore components all activate the MAD-dependent checkpoint. In contrast, the cell cycle arrest caused by mutations that induce DNA damage (cdc13), inactivate the cyclin proteolysis machinery (cdc16 and cdc23), or arrest cells in anaphase (cdc15) is independent of the spindle checkpoint.
Hardwick
K G
KG
Department of Physiology, University of California, San Francisco, California 94143-0444, USA.
Li
R
R
Mistrot
C
C
Chen
R H
RH
Dann
P
P
Rudner
A
A
Murray
A W
AW
D002455:Cell Division / Q000235:genetics; D016213:Cyclins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D018816:Genes, cdc / Q000235:genetics; D006868:Hydrolysis; D018386:Kinetochores; D008870:Microtubules / Q000235:genetics; D020409:Molecular Motor Proteins / Q000235:genetics; D009154:Mutation; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D008941:Spindle Apparatus / Q000235:genetics*; D014404:Tubulin / Q000235:genetics
D016213:Cyclins; D004268:DNA-Binding Proteins; D020409:Molecular Motor Proteins; D012097:Repressor Proteins; D014404:Tubulin