Identification of genes directly and indirectly involved in the insect transmission of African cassava mosaic geminivirus by Bemisia tabaci.
The inability to transmit progeny virus resulting from the cloned components of an isolate of African cassava mosaic virus originating from Kenya (ACMV-K) has been shown to be due to defects in both genomic components. This was achieved by the production of infectious pseudorecombinants between ACMV-K and the cloned components of a whitefly-transmissible ACMV isolate originating from Nigeria (ACMV-NOg). The exchange of gene fragments between ACMV-K and ACMV-NOg has been used to demonstrate that the defects responsible for lack of transmissibility reside on the coat protein and DNA B C1 gene of ACMV-K. The significance of these finding with respect to the present understanding of the function of these gene products are discussed.
Liu
S
S
Department of Virus Research, John Innes Centre, Norwich, UK.
Briddon
R W
RW
Bedford
I D
ID
Pinner
M S
MS
Markham
P G
PG
D000818:Animals; D002213:Capsid / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D005814:Genes, Viral; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D002365:Manihot / Q000821:virology*; D010935:Plant Diseases; D018515:Plant Leaves / Q000821:virology; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
D011994:Recombinant Proteins; D014764:Viral Proteins