title: Inhibitory effects of combined treatment with vitamin K and D on bone loss of ovariectomized rats: a microradiographic study.
abstract: This study was designed to assess the effect of vitamin K and D supplementation on ovariectomy-induced bone loss. Female Sprague-Dawley rats aged 8-9 months were ovariectomized (OVX) or sham operated and divided into five experimental groups: (1) sham operation, (2) ovariectomy (OVX), (3) OVX plus vitamin K supplementation, (4) OVX plus vitamin D supplementation, (5) OVX plus vitamin K and vitamin D supplementation. The trabecular bone area was estimated by bone histomorphometry by microradiography and histological examination. Bone loss in OVX plus vitamin K and vitamin D group was significantly reduced at both 7 and 14 weeks compared with the OVX group. No significant bone loss in OVX plus vitamin K or OVX plus vitamin D groups was found. Similar effect of vitamin K and D supplementation on ovariectomy-induced bone loss was recognized in histological examination. Our findings indicate that vitamin K and D may have synergistic effects on reducing bone loss. This is a valuable information for the treatment of bone loss in postmenopausal women with osteoporosis.
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University.
authors.forename: K
authors.initials: K
authors.lastname: Kamezawa
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D008852:Microradiography; D015663:Osteoporosis, Postmenopausal / Q000000981:diagnostic imaging / Q000188:drug therapy*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014807:Vitamin D / Q000627:therapeutic use*; D014812:Vitamin K / Q000627:therapeutic use*
chemical_list: D014812:Vitamin K; D014807:Vitamin D