Methylation and silencing of the Thrombospondin-1 promoter in human cancer.
Neovascularization is a common feature of many human cancers, but relatively few molecular defects have been demonstrated in genes regulating angiogenesis. Decreased expression of Thrombospondin-1 (THBS1), a P53 and Rb regulated angiogenesis inhibitor, has been observed in some human tumors, including glioblastoma multiforme (GBM). To study whether methylation-associated inactivation is involved in down-regulating THBS1 expression in cancer, we analysed the methylation status of THBS1 in several cell lines and primary tumors. Three cell lines (RKO, CEM and RAJI) were completely methylated at several CpG sites within the THBS1 5' CpG island, and had no detectable expression by RT-PCR. THBS1 expression was readily reactivated using the methylation-inhibitor 5-deoxy-azacytidine in all three lines. Furthermore, THBS1 methylation was present in 33% (14/42) of primary GBMs. Thus, de novo methylation may serve as a potential way to inactivate THBS1 expression in human neoplasms.
Li
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Q
The Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
Ahuja
N
N
Burger
P C
PC
Issa
J P
JP
D001932:Brain Neoplasms / Q000235:genetics*; D018938:Caco-2 Cells; D018899:CpG Islands; D019175:DNA Methylation; D005909:Glioblastoma / Q000235:genetics*; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D011401:Promoter Regions, Genetic; D019700:Thrombospondin 1 / Q000235:genetics*; D014407:Tumor Cells, Cultured
D019700:Thrombospondin 1