title: Cloning of a cysteine proteinase gene of Theileria sergenti.
abstract: A cDNA encoding cysteine proteinase of Theileria sergenti was isolated from a piroplasm cDNA library and its nucleotide sequence was determined. The gene encodes a polypeptide of 402 amino acids with predicted molecular mass of 46.4 kDa. Analysis of the predicted amino acid sequence revealed a number of features common to known cysteine proteinases. Southern blot analysis showed that the cysteine proteinase gene was likely to be a single copy per genome.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sako
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: M
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016794:Theileria / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D003546:Cysteine Endopeptidases