title: Endurance training increases the beta-adrenergic lipolytic response in subcutaneous adipose tissue in obese subjects.
abstract: OBJECTIVE
  The aim of this study was to assess, by longitudinal follow-up, the influence of aerobic training on the in vivo lipolytic activity of adipose tissue in obese male subjects.
  
  
  METHODS
  Eleven obese non-diabetic males, aged 41.5+/-5.77 (range 27-49 y) with body mass index (BMI) 36.5+/-4.5 kg/m2 (range 29.4-47.1 kg/m2) participated in the study.
  
  
  METHODS
  Subjects took part in a 12-week aerobic training program. Before and after training, microdialysis of abdominal subcutaneous adipose tissue (SCAT) was carried out, using perfusion with graded doses of the beta-adrenergic agonist isoprenaline and a single dose of the phosphodiesterase inhibitor theophylline. In addition, the response of plasma glycerol and free fatty acids (FFAs) to intravenous infusion of graded doses of isoprenaline was tested.
  
  
  RESULTS
  The training did not induce significant weight loss and promoted an increase in maximum aerobic capacity (P<0.05). The increase of extracellular glycerol in SCAT in response to isoprenaline perfusion was enhanced after the training (P<0.05), while no change in the response of interstitial glycerol to theophylline action was observed. The training did not elicit a change in the isoprenaline-induced changes of blood flow in adipose tissue. The increases of plasma FFAs and glycerol in response to intravenous isoprenaline infusion, were significantly enhanced after training.
  
  
  CONCLUSIONS
  The present study shows that aerobic training induced an increase in the response of plasma and subcutaneous adipose tissue concentration of glycerol to beta-adrenergic stimulation. The effect of an agent acting at the post-receptor level (theophylline) in SCAT was not modified by training.
authors.affiliation: Department of Sport Medicine, 3rd Medical Faculty, Charles University, Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Stich
authors.forename: I
authors.initials: I
authors.lastname: de Glisezinski
authors.forename: J
authors.initials: J
authors.lastname: Galitzky
authors.forename: J
authors.initials: J
authors.lastname: Hejnova
authors.forename: F
authors.initials: F
authors.lastname: Crampes
authors.forename: D
authors.initials: D
authors.lastname: Rivière
authors.forename: M
authors.initials: M
authors.lastname: Berlan
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000008:administration & dosage; D000328:Adult; D015444:Exercise; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005951:Glucose Tolerance Test; D005990:Glycerol / Q000097:blood; D006801:Humans; D007545:Isoproterenol / Q000008:administration & dosage; D008055:Lipids / Q000097:blood; D008066:Lipolysis; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism* / Q000628:therapy; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage; D010807:Physical Endurance; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D013806:Theophylline / Q000008:administration & dosage
chemical_list: D000318:Adrenergic beta-Agonists; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D010726:Phosphodiesterase Inhibitors; D011943:Receptors, Adrenergic, beta; D013806:Theophylline; D007545:Isoproterenol; D005990:Glycerol