Molecular cloning of an acetylcholinesterase gene from the plant parasitic nematodes, Meloidogyne incognita and Meloidogyne javanica.
A gene encoding a protein with strong homology with Caenorhabditis elegans and C. briggsae acetylcholinesterase ACE-1 was cloned from Meloidogyne incognita and M. javanica pre-parasitic juveniles. Both cDNAs have an ORF of 1968 bp for a deduced translation product of 656 amino acid residues. The key residues essential to acetylcholinesterase (AChE) structure and function are conserved in both sequences. M. incognita and M. javanica AChE share a homology of 98.8% at the amino acid level and 97% at the nucleotide level. Phylogenetic analysis showed that Meloidogyne and Caenorhabditis AChE form a cluster among AChE of triploblastic organisms. This Meloidogyne AChE is expressed in eggs, pre-parasitic juveniles and males and AChE activity was detected in situ in amphids of pre-parasitic juveniles. The opportunity of using AChE as a target in new strategies of nematode control is discussed.
Piotte
C
C
Laboratoire de Biologie des Invertébrés, INRA, Antibes, France.
Arthaud
L
L
Abad
P
P
Rosso
M N
MN
D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D017238:Genes, Helminth; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000469:parasitology; D016415:Sequence Alignment; D014431:Tylenchoidea / Q000201:enzymology / Q000235:genetics*
D000110:Acetylcholinesterase