title: Oxalate oxidase from Ceriporiopsis subvermispora: biochemical and cytochemical studies.
abstract: The enzyme oxalate oxidase was identified in mycelial extracts of the basidiomycete Ceriporiopsis subvermispora and thereafter purified to homogeneity. The purification procedure included only three steps: Q-Sepharose chromatography, precipitation at pH 3.0, and phosphocellulose chromatography. The enzyme is a 400-kDa homohexamer, as determined by gel permeation in Sephadex G-200 and SDS-polyacrylamide gel electrophoresis. Isoelectrofocusing revealed a pI of 4.2. Optimal activity was obtained at pH 3.5 and at 45 degrees C. The purified enzyme has Km and kcat values of 0.1 mM and 88 s-1, respectively. It is highly specific for oxalate, although it is inhibited at concentrations of this substrate above 2.5 mM. Hystochemistry studies conducted over mycelium slices showed reactions products in both endocellular and periplasmic associated elements. A possible connection between the intracellular metabolism of oxalate and the extracellular ligninolytic activity of the fungus is proposed.
authors.affiliation: Departamento de Genética Molecular y Microbiología, Departamento de Biología Celular y Molecular, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Aguilar
authors.forename: U
authors.initials: U
authors.lastname: Urzúa
authors.forename: C
authors.initials: C
authors.lastname: Koenig
authors.forename: R
authors.initials: R
authors.lastname: Vicuña
mesh_terms: D004789:Enzyme Activation; D006651:Histocytochemistry; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology; D011124:Polyporaceae / Q000201:enzymology* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D010940:Plant Proteins; D010088:Oxidoreductases; C020598:oxalate oxidase