Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene.
To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR.
Kubota
S
S
Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, Japan.
Hattori
T
T
Nakanishi
T
T
Takigawa
M
M
D020413:3' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D055513:Connective Tissue Growth Factor; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006133:Growth Substances / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D020413:3' Untranslated Regions; C526317:CCN2 protein, human; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor