[Diagnosis of the active form of cytomegalovirus infection based on determination of IgG antibodies to the immediate-early cytomegalovirus protein by lanthanide immunofluorescence analysis].
A system for time-resolved fluoroimmunoassay (TR-FIA) is developed for detecting early IgG during the active stage of cytomegalovirus infection on the basis of CMV-control enzyme immunoassay system. The antigen is cloned and expressed in E. coli recombinant main immediate early protein of human CMV. The active form of CMV infection was detected additionally in 5 out of 25 women who gave birth to sick babies by means of the new test system in complex with other laboratory diagnostic methods. The test is recommended for prenatal diagnosis of active CMV infection in pregnant women and for predicting the risk of neonatal disease.
Pomelova
V G
VG
Zubkov
V V
VV
Bychenkova
T A
TA
Osin
N S
NS
Kudashov
N I
NI
Zvonarev
A Iu
AIu
Kuliakina
M N
MN
Zaĭtsev
I Z
IZ
D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D017874:Immediate-Early Proteins / Q000276:immunology*; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000276:immunology; D008674:Metals, Rare Earth; D011247:Pregnancy; D011296:Prenatal Diagnosis
D017874:Immediate-Early Proteins; D008674:Metals, Rare Earth