Selective PCR amplification of functional immunoglobulin light chain from hybridoma containing the aberrant MOPC 21-derived V kappa by PNA-mediated PCR clamping.
Cochet
O
O
Institut Curie-Unité INSERM 255, Paris, France.
Martin
E
E
Fridman
W H
WH
Teillaud
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D017931:DNA Primers / Q000235:genetics; D006825:Hybridomas / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology; D020135:Peptide Nucleic Acids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D020135:Peptide Nucleic Acids