Identification and characterization of a protein containing formin homology (FH1/FH2) domains.
A novel member of the Formin/Diaphanous family of proteins was cloned and characterized. A 4kB mRNA is ubiquitously expressed but is found in abundance in the spleen. FHOS (Formin Homologue Overexpressed in Spleen) contains a 3414bp open reading frame and encodes for an approximately 128kDa protein. FHOS has sequence homology to Diaphanous and Formin proteins within the Formin Homology (FH)1 and FH2 domains. FHOS also contains a coiled-coil, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. FHOS-specific antiserum was generated and used to determine that FHOS is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. FHOS was mapped to chromosome 16q22 between framework markers WI-5594 and WI-9392.
Westendorf
J J
JJ
Vanderbilt University, Department of Biochemistry and Vanderbilt Cancer Center, Nashville, TN 37232, USA.
Mernaugh
R
R
Hiebert
S W
SW
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D005326:Fetal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D000080865:Formins; D018922:HL-60 Cells; D006801:Humans; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D012313:RNA / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D014407:Tumor Cells, Cultured
C120021:FHOD1 protein, human; D005326:Fetal Proteins; D000080865:Formins; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D012313:RNA