PAX6 expression in the developing human eye.
OBJECTIVE
To investigate the changes in PAX6 expression in the developing human eye.


METHODS
Six developing human eyes from 6 to 22 weeks' gestation were evaluated. Frozen sections were immunohistochemically stained with monoclonal antibody to chick Pax6 (amino acids 1-223). To verify antibody specificity, western blot analysis was carried out using cell lysates from P19 cells transfected with the human PAX6 gene.


RESULTS
Western blot analysis demonstrated that the antibody reacted to human PAX6 protein. Positive immunostainings for PAX6 were seen in the surface ectoderm, lens vesicle, inner and outer layers of the optic cup, and optic stalk at 6 weeks, and in the corneal epithelia and conjunctiva, lens, and non-pigmented ciliary epithelia from 8 to 22 weeks. In the retina, positive cells were seen in the entire retina from 8 to 10 weeks, and were restricted to the ganglion cell layer and the inner and outer portions of the inner nuclear layer after 21 weeks.


CONCLUSIONS
PAX6 is expressed on the surface and neuroectoderms at an early stage, then in the differentiating cells in the cornea, lens, ciliary body, and retina through development. PAX6 may play a role in determining cell fate in the morphogenesis of various human ocular tissue.
Nishina
S
S
Department of Ophthalmology, National Children's Hospital, Tokyo, Japan.
Kohsaka
S
S
Yamaguchi
Y
Y
Handa
H
H
Kawakami
A
A
Fujisawa
H
H
Azuma
N
N
D000918:Antibody Specificity; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000235:genetics*; D005123:Eye / Q000196:embryology*; D005136:Eye Proteins; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D018398:Homeodomain Proteins; D006801:Humans; D007150:Immunohistochemistry; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins