title: Identification and characterisation of a cytochrome P450 gene and processed pseudogene from an arachnid: the cattle tick, Boophilus microplus.
abstract: We isolated and sequenced the first known cytochrome P450 gene and pseudogene from an arachnid, the cattle tick, Boophilus microplus. Both the gene and pseudogene belong to the family CYP4, but a new subfamily, CYP4W, had to be created for these genes because they are substantially different to other CYP4 genes. The gene, CYP4W1, has greatest homology with CYP4C1 from a cockroach, Blaberus discoidalis. The predicted molecular weight of the protein encoded by CYP4W1 (63 KDa) is greater than that of the other CYP4 genes. The pseudogene, CYP4W1P, is probably a processed pseudogene derived from the functional gene CYP4W1. This is only the third CYP processed pseudogene to be identified. The pseudogene is 98% identical to the functional gene, CYP4W1, therefore we hypothesise that this pseudogene evolved recently from the functional gene. The CYP4 genes from arthropods have diverged from each other more than those of mammals; consequently the phylogeny of the arthropod genes could not be resolved.
authors.affiliation: Department of Parasitology, University of Queensland, Brisbane, Australia. andrea@cybergal.com
authors.forename: A L
authors.initials: AL
authors.lastname: Crampton
authors.forename: G D
authors.initials: GD
authors.lastname: Baxter
authors.forename: S C
authors.initials: SC
authors.lastname: Barker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010802:Phylogeny; D011544:Pseudogenes; D013987:Ticks / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D003577:Cytochrome P-450 Enzyme System