MCP-1 is selectively expressed in the late phase by cytokine-stimulated human neutrophils: TNF-alpha plays a role in maximal MCP-1 mRNA expression.
Culture supernatants of phytohemagglutinin (PHA)-stimulated human peripheral blood mononuclear cells (PHA-sup) induced monocyte chemoattractant protein-1 (MCP-1) mRNA expression in human neutrophils. MCP-1 mRNA was first detected by Northern analysis at 8 h, and the peak level was detected at 16 h and sustained until 72 h. Cycloheximide and genistein, but not pertussis toxin, inhibited the expression of MCP-1 mRNA. Recombinant tumor necrosis factor alpha (TNF-alpha) induced a low level MCP-1 mRNA accumulation in neutrophils, and addition of anti-TNF-alpha IgG blocked 30-70% of MCP-1 mRNA expression induced with PHA-sup. PHA-sup-stimulated PMN synthesized and secreted 3.1+/-1.3 ng/5 x 10(6) PMN MCP-1 within the first 24 h. Hybridization of 32P-labeled cDNA preparations to an array of human cytokine cDNAs further indicated that MCP-1 mRNA was selectively up-regulated in the late phase after stimulation with the PHA-sup.
Yamashiro
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S
Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702, USA.
Kamohara
H
H
Yoshimura
T
T
D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D037342:Pertussis Toxin; D010835:Phytohemagglutinins / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D015854:Up-Regulation / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D018932:Chemokine CCL2; D016207:Cytokines; D018076:DNA, Complementary; D006131:Growth Inhibitors; D010835:Phytohemagglutinins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D010566:Virulence Factors, Bordetella; D003513:Cycloheximide; D019833:Genistein; D037342:Pertussis Toxin