title: Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
abstract: The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohba
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Nishitani
authors.forename: T
authors.initials: T
authors.lastname: Nishimoto
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D005260:Female; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008297:Male; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D010063:Ovum; D011994:Recombinant Proteins / Q000378:metabolism; D013077:Sperm Head / Q000502:physiology; D008941:Spindle Apparatus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D014981:Xenopus; D029867:Xenopus Proteins; D020931:ran GTP-Binding Protein
chemical_list: D018797:Cell Cycle Proteins; D002457:Cell Extracts; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; C489079:NUMA1 protein, Xenopus; D009687:Nuclear Proteins; C499304:RCC1 protein, Xenopus; D011994:Recombinant Proteins; D029867:Xenopus Proteins; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D004398:Dyneins; D020931:ran GTP-Binding Protein