title: Pulmonary capillary sieving of hetastarch is not altered by LPS-induced sepsis.
abstract: BACKGROUND
  Gram-negative lipopolysaccharide (LPS) has been demonstrated to increase pulmonary capillary permeability as judged by the increased flow of protein-rich lymph from the lungs of sheep infused with LPS. This finding suggests that LPS-injured pulmonary capillaries might be less restrictive than uninjured capillaries to the filtration of large hetastarch molecules. Hetastarch has a broad molecular mass spectrum (35-1,500 kilodaltons (kDa)), and one way to test the restrictiveness of pulmonary capillaries is to measure the size of the largest hetastarch molecules that cross the microvascular barrier and enter the lymph. To evaluate the effects of LPS, we compared hetastarch molecular distributions in the lung lymph of normal and LPS-injured sheep.
  
  
  METHODS
  Adult sheep (38.2 +/- 0.8 kg) were surgically prepared for the collection of lung lymph, with study initiation after a 5- to 7-day recovery period. Hetastarch (6%) was infused (10 mL/kg) 24 hours before study to allow for stabilization of the hetastarch molecular distribution. On the day of study, LPS (Escherichia coli lipopolysaccharide, 2 microg/kg; n = 6) was infused, and plasma and lymph samples were collected for 12 hours. An additional group of animals not infused with LPS (n = 6) served as controls. Hetastarch molecular distributions in plasma and lymph were measured by using high performance size exclusion chromatography.
  
  
  RESULTS
  In control sheep, the largest hetastarch molecules in lymph averaged 861 +/- 18 kDa (mean +/- SEM) (plasma, 1,065 +/- 18 kDa). In LPS-treated sheep, the largest hetastarch molecules in lymph averaged 845 +/- 19 kDa (not significant vs. normal) (plasma, 1,025 +/- 14 kDa). Hetastarch concentrations in plasma and lung lymph of normal sheep, respectively, were 0.61 +/- 0.05% and 0.34 +/- 0.07%. In LPS-treated sheep, hetastarch concentrations in plasma and lymph were 0.56 +/- 0.08 (not significant vs. normal) and 0.29 +/- 0.07, respectively (p < or = 0.05). Lymph concentrations were lower after LPS because of increased lymph flows (19.9 +/- 5.4 mL/30 min, compared with 3.6 +/- 0.8 mL/30 min in normal sheep).
  
  
  CONCLUSIONS
  Our results suggest that LPS does not alter the diameter of the largest pores perforating the walls of pulmonary capillaries. Rather, the number of these pores in the capillary wall appears to be increased. This increase would explain why lymph flows rise after LPS with little change in the lymph protein concentration. Our results are also consistent with a filtration model in which capillaries are assumed to be perforated by small pores (protein reflection coefficient = 1) as well as large pores (protein reflection coefficient = 0).
authors.affiliation: Department of Surgery, University of Wisconsin-Madison Medical School Madison, USA. rconhaim@facstaff.wisc.edu
authors.forename: R L
authors.initials: RL
authors.lastname: Conhaim
authors.forename: K E
authors.initials: KE
authors.lastname: Watson
authors.forename: B M
authors.initials: BM
authors.lastname: Potenza
authors.forename: B A
authors.initials: BA
authors.lastname: Harms
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D004926:Escherichia coli; D006439:Hemodynamics; D006895:Hydroxyethyl Starch Derivatives / Q000493:pharmacokinetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000098:blood supply*; D008196:Lymph / Q000737:chemistry / Q000502:physiology; D008970:Molecular Weight; D018805:Sepsis / Q000209:etiology / Q000503:physiopathology*; D012756:Sheep
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D008070:Lipopolysaccharides