Butyrylcholinesterase is complexed with transferrin in chicken serum.
The function of the enzyme butyrylcholinesterase (BChE) both in serum and in brain is unclear. In serum, BChE has been found complexed with several biomedically relevant proteins, with which it could function in concert. Here, the existence of a similar complex formed between BChE and sero-transferrin from adult chicken serum was elucidated. In order to identify both proteins unequivocally, we improved methods to highly purify the 81-kDa BChE and the coisolated 75-kDa transferrin, which then allowed us to tryptically digest and sequence the resulting peptides. The sequences as revealed for BChE peptides were highly identical to mammalian BChEs. A tight complex formation between the two proteins could be established (a) since transferrin is coisolated along with BChE over three steps including procainamide affinity chromatography, while transferrin alone is not bound to this affinity column, and (b) since imunoprecipitation experiments of whole serum with a transferrin-specific antiserum allows us to detect BChE in the precipitate with the BChE-specific monoclonal antibody 7D11. The possible biomedical implications of a complex between transferrin and BChE which here has been shown to exist in chicken serum are briefly discussed.
Weitnauer
E
E
Department of Developmental Biology and Neurogenetics, Darmstadt University of Technology, Germany.
Ebert
C
C
Hucho
F
F
Robitzki
A
A
Weise
C
C
Layer
P G
PG
D000595:Amino Acid Sequence; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008322:Mammals; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011233:Precipitin Tests; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014168:Transferrin / Q000302:isolation & purification / Q000378:metabolism*
D010446:Peptide Fragments; D014168:Transferrin; D002091:Butyrylcholinesterase