title: Detection of bluetongue virus genome segment 6 sequences by RT-PCR.
abstract: Reverse transcription-PCR (RT-PCR) technique was adopted to amplify a 101 basepair nucleotide sequence of bluetongue virus (BTV) genome segment 6. The specificity of the amplicon was determined by its approximate size in 3% agarose gel electrophoresis, digestion with restriction enzyme MspI, dot-blot hybridization and cycle sequencing. The technique was found to be suitable for detection of bluetongue virus in infected cell culture and clinical samples.
authors.affiliation: National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Bandyopadhyay
authors.forename: R S
authors.initials: RS
authors.lastname: Kataria
authors.forename: A K
authors.initials: AK
authors.lastname: Tiwari
mesh_terms: D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics*; D002478:Cells, Cultured; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction