In vivo fate and immune pulmonary response after nasal administration of microspheres loaded with phosphorylcholine-thyroglobulin.
Phosphorylcholine is a widely occurring hapten which is present in the cell wall of many prokaryotes. It is, therefore, an attractive candidate for the development of a vaccine against many bacterial diseases. Poly(D,L-lactide-co-glycolide) microspheres loaded with phosphorylcholine linked to thyroglobulin (PC-Thyr) as protein carrier were prepared. The effect of the protein concentration on antigen encapsulation and release as well as on microsphere morphology has been investigated. When administered intranasally, PC-Thyr-loaded microspheres were taken up by epithelial cells of the nasopharyngeal associated lymphoid tissue and induced a specific IgA and IgG response in pulmonary secretions as well as a strong systemic immune response in BALB/c mice.
Trolle
S
S
URA-CNRS 1218, Faculté de Pharmacie, 92296, Châtenay-Malabry Cedex, France.
Caudron
E
E
Leo
E
E
Couvreur
P
P
Andremont
A
A
Fattal
E
E
D000281:Administration, Intranasal; D000818:Animals; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood; D008168:Lung / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010316:Particle Size; D010767:Phosphorylcholine / Q000276:immunology*; D013954:Thyroglobulin / Q000008:administration & dosage / Q000276:immunology*
D007070:Immunoglobulin A; D010767:Phosphorylcholine; D013954:Thyroglobulin