Mannan-degrading enzymes from Cellulomonas fimi.
The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and beta-mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian beta-mannosidases.
Stoll
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Department of Microbiology and Immunology and The Protein Engineering Network of Centres of Excellence, The University of British Columbia, Vancouver, British Columbia, Canada.
Stålbrand
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Warren
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D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D015723:Gene Library; D006093:Gram-Positive Asporogenous Rods / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D008351:Mannans / Q000378:metabolism*; D008361:Mannosidases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D044902:beta-Mannosidase
D004269:DNA, Bacterial; D008351:Mannans; D008361:Mannosidases; D044902:beta-Mannosidase; C041867:endo-1,4-beta-D-mannanase