Release of outer membrane vesicles from Bordetella pertussis.
The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly- showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.
Hozbor
D
D
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CONICET Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Rodriguez
M E
ME
Fernández
J
J
Lagares
A
A
Guiso
N
N
Yantorno
O
O
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins / Q000032:analysis; D015153:Blotting, Western; D001886:Bordetella pertussis / Q000648:ultrastructure*; D018699:Coated Vesicles / Q000737:chemistry / Q000378:metabolism*; D006388:Hemagglutinins / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis; D008854:Microscopy, Electron; D011498:Protein Precursors / Q000032:analysis; D010566:Virulence Factors, Bordetella / Q000032:analysis
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D006388:Hemagglutinins; D008070:Lipopolysaccharides; D011498:Protein Precursors; D010566:Virulence Factors, Bordetella