Improved fusion partners transfected with DNA fragment encoding IL-11 on generation of human B lymphocyte hybridomas.
IL-11, a less identified cytokine, possesses some overlapping functions with IL-6 that are able to facilitate the growth and antibody secretion of B lymphocyte hybridomas. In this report, a DNA fragment encoding human IL-11 was transduced into fusion partners (mouse myelomas Ag8.653 and SP2/0, and human lymphoblastoid cell line HF2) mediated by lipofection. The transfected cells selected with G418 secreted IL-11 constitutively over the range of 32.4 +/- 10.5 units/ml to 76.6 +/- 18.4 units/ml, which could be inhibited by an IL-11 neutralizing MAb up to 80%. The fusion frequency of PBMC doubled, while that of LCLs displayed a 2.4- or 3.3-fold increase, when fused with the transfected fusion partners, respectively. The derived hybridomas from IL-11 secreting fusion partner secreted 3 or 4 times as many immunoglobulins as that from its ancestor. Our data indicate that IL-11 gene transfected fusion partners are improved cell lines for generation of human B lymphocyte hybridomas, and IL-11 may contribute to the increased fusion frequency and antibody secretion of B lymphocyte hybridomas.
Zhu
Y
Y
Department of Immunology, Fourth Military Medical University, Shaanxi, P.R. China. zhuyong@ihw.com.cn
Jin
B
B
Jiang
S
S
Sun
K
K
Sun
C
C
Liu
X
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D002459:Cell Fusion; D002478:Cells, Cultured; D005822:Genetic Vectors; D006801:Humans; D006825:Hybridomas; D007136:Immunoglobulins / Q000096:biosynthesis*; D017370:Interleukin-11 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D017370:Interleukin-11