title: Effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test.
abstract: Since 1990, red sea bream iridovirus (RSIV) has caused high mortalities in the summertime in cultured red sea bream Pagrus major in southwest Japan. To establish control measures for red sea bream iridoviral disease (RSIVD), the effectiveness of a formalin-killed viral vaccine was evaluated in a field trial. Two groups each consisting of 1000 juvenile red sea bream were either intraperitoneally inoculated with vaccine (vaccinated group) or were not vaccinated (non-vaccinated group). After vaccination, the fish were held for 1 wk, then transferred to a marine net pen and observed for 12 wk. The cumulative mortalities caused by RSIVD in the vaccinated group or control group were 19.2 and 68.5%, respectively. Additionally, the presence of virus antigen in the spleen was investigated and body weight was measured 6 and 12 wk post vaccination. In the vaccinated group, viral antigen was not detected. The increase in body weight of vaccinated fish was significantly (p < 0.05) greater than that of control fish. These results suggest that the vaccine against RSIVD was effective in 1 field trial.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Agency, Mie, Japan. kazuhiro@nria.affrc.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Maeno
authors.forename: A
authors.initials: A
authors.lastname: Honda
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
authors.forename: T
authors.initials: T
authors.lastname: Tooriyama
authors.forename: S
authors.initials: S
authors.lastname: Manabe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D005399:Fishes; D007274:Injections, Intraperitoneal / Q000662:veterinary; D017994:Iridovirus / Q000276:immunology*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D013158:Splenic Diseases / Q000517:prevention & control / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
chemical_list: D000911:Antibodies, Monoclonal; D015164:Vaccines, Inactivated; D014765:Viral Vaccines