Molecular heterogeneity and regulation of poly(ADP-ribose) glycohydrolase.
We have recently described the isolation and characterization of bovine cDNA encoding poly(ADP-ribose) glycohydrolase (PARG). We describe here the preparation and characterization of antibodies to PARG. These antibodies have been used to demonstrate the presence of multiple forms of PARG in tissue and cell extracts from bovine, rat, mouse, and insects. Our results indicate that multiple forms of PARG previously reported could result from a single gene. Analysis of PARG in cells in which poly(ADP-ribose) polymerase (PARP) has been genetically inactivated indicates that the cellular content of PARG is regulated independently of PARP.
Amé
J C
JC
Division of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington 40536-0082, USA.
Jacobson
E L
EL
Jacobson
M K
MK
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D005838:Genotype; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D008957:Models, Genetic; D051381:Rats; D011993:Recombinant Fusion Proteins; D014407:Tumor Cells, Cultured
D011993:Recombinant Fusion Proteins; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase