title: Double staining of skeleton using microwave irradiation.
abstract: The fetal skeleton double staining method is used to reveal developmental abnormalities in the skeletal system. We used alizarin red S and alcian blue successfully with microwave irradiation for skeletal double staining. The fixation time was reduced from 4-7 days to 2-2.5 min and the staining time was reduced from 4 days to 23 min.
authors.affiliation: Department of Histology and Embryology, Gazi University, Ankara, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Ilgaz
authors.forename: H
authors.initials: H
authors.lastname: Kocabiyik
authors.forename: D
authors.initials: D
authors.lastname: Erdogan
authors.forename: C
authors.initials: C
authors.lastname: Ozogul
authors.forename: T
authors.initials: T
authors.lastname: Peker
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000196:embryology*; D005260:Female; D008297:Male; D051379:Mice; D008872:Microwaves; D013194:Staining and Labeling / Q000379:methods*