Structural heterogeneity of the sialic-acid-containing oligosaccharides from the lipopolysaccharide of Hafnia alvei strain 2 as detected by FABMS studies.
The structure of four oligosaccharide fractions from the Hafnia alvei strain 2 lipopolysaccharide (LPS) have been assigned by FABMS. This approach corroborates data previously established by NMR spectroscopy for the major oligosaccharides in these fractions [A. Gamian, E. Romanowska, U. Dabrowski, J. Dabrowski, Biochemistry 30 (1991) 5032-5038; E. Katzenellenbogen, A. Gamian, E. Romanowska, U. Dabrowski, J. Dabrowski, Biochem. Biophys. Res. Commun. 194 (1993) 1058-1064; N. Ravenscroft, A. Gamian, E. Romanowska, Eur. J. Biochem. 227 (1995) 889-896]. In addition, the MS/MS with B/E linked scan technique allowed the detection of an additional oligosaccharide with the structure: [formula: see text] lacking the branched O-6 linked glucopyranose residue at the 3-linked Gal unit, which indicates a structural heterogeneity for the major oligosaccharide fraction.
Gamian
A
A
Polish Academy of Sciences, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland. gamian@immuno.iitd.pan.wroc.pl
Ulrich
J
J
Defaye
J
J
Mieszała
M
M
Witkowska
D
D
Romanowska
E
E
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004755:Enterobacteriaceae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry
D008070:Lipopolysaccharides; D009844:Oligosaccharides; D019158:N-Acetylneuraminic Acid