Isolation and characterization of two cDNA clones encoding for glutamate dehydrogenase in Nicotiana plumbaginifolia.
We have isolated two full length cDNA clones encoding Nicotiana plumbaginifolia NADH-glutamate dehydrogenase. Both clones share amino acid boxes of homology corresponding to conserved GDH catalytic domains and putative mitochondrial targeting sequence. One clone shows a putative EF-hand loop. The level of the two transcripts is affected differently by carbon source.
Ficarelli
A
A
Institute of Genetics, University of Parma, Italy.
Tassi
F
F
Restivo
F M
FM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D005969:Glutamate Dehydrogenase