An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies.
A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi-solid media is described. The accumulation of metaphases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly-L-lysine-treated slides and fixed stepwise prior to staining. The technique produced high yields of well-spread metaphases facilitating clonal cytogenetic analysis.
Kadhim
M A
MA
Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire.
Pocock
D A
DA
Lorimore
S A
SA
Wright
E G
EG
D000818:Animals; D002875:Chromosomes; D003582:Cytogenetics / Q000379:methods*; D006412:Hematopoietic Stem Cells; D051379:Mice