title: Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin.
abstract: Using the baculovirus/Sf9 cell expression system, we have incorporated 99% 15N-enriched [alpha,epsilon-15N2]-L-lysine into the rod visual pigment rhodopsin. We have subsequently investigated the protonated Schiff base (pSB) linkage in the [alpha, epsilon-15N2]Lys-rhodopsin with cross-polarization magic angle spinning (CP/MAS) 15N NMR. The Schiff base (SB) 15N in [alpha, epsilon-15N2]Lys-rhodopsin resonates with an isotropic shift sigmaI of 155.9 ppm, relative to 5.6 M 15NH4Cl. This suggests that the SB in rhodopsin is protonated and stabilized by a complex counterion. The 15N shifts of retinal SBs correlate with the energy difference between the ground and excited states and the frequency of maximum visible absorbance, numax, associated with the pi-pi transition of the polyene chromophore. Experimental modeling of the relation between the numax and the size of the counterion with a set of pSBs provides strong evidence that the charged chromophore in rhodopsin is stabilized by a counterion with an estimated effective center-center distance (deff) between the counterion and the pSB of 0.43 +/- 0.01 nm. While selected prokaryotic proteins and complexes have been labeled before, this is the first time to our knowledge that a 15N-labeled eukaryotic membrane protein has been generated in sufficient amount for such NMR investigations.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, RA Leiden, The Netherlands.
authors.forename: A F
authors.initials: AF
authors.lastname: Creemers
authors.forename: C H
authors.initials: CH
authors.lastname: Klaassen
authors.forename: P H
authors.initials: PH
authors.lastname: Bovee-Geurts
authors.forename: R
authors.initials: R
authors.lastname: Kelle
authors.forename: U
authors.initials: U
authors.lastname: Kragl
authors.forename: J
authors.initials: J
authors.lastname: Raap
authors.forename: W J
authors.initials: WJ
authors.lastname: de Grip
authors.forename: J
authors.initials: J
authors.lastname: Lugtenburg
authors.forename: H J
authors.initials: HJ
authors.lastname: de Groot
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D019204:GTP-Binding Proteins / Q000737:chemistry*; D007553:Isotope Labeling; D008956:Models, Chemical; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D020419:Photoreceptor Cells, Vertebrate / Q000737:chemistry*; D012172:Retinaldehyde / Q000737:chemistry; D012243:Rhodopsin / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D009587:Nitrogen Isotopes; D012545:Schiff Bases; D012243:Rhodopsin; D019204:GTP-Binding Proteins; D012172:Retinaldehyde