[Screening for mitochondrial 1555(G) mutation in patients with aminoglycoside antibiotic-induced deafness].
OBJECTIVE
To identify the incidence of the 1555(G) mutation in pedigrees and sporadic patients with aminoglycoside antibiotic- induced deafness so as, to privide the theoretical evidence for establishing the method of diagnosis of this disease.


METHODS
Blood samples were obtained from two pedigrees and seven sporadic patients with aminoglycoside antibiotic-induced deafness, and five mothers of the sporadic patients. DNA was extracted from the isolated leukocytes. The mitochondrial DNA fragments were amplified by PCR; 1555(G) mutation was detected by Alw26 I restriction endonuclease digestion.


RESULTS
Fourteen individuals from two pedigrees carried homoplasmic 1555(G) mutation. Seven sporadic patients and the five mothers did not have 1555(G) mutation.


CONCLUSIONS
The incidence of the 1555(G) mutation in pedigrees with aminoglycoside antibiotic-induced deafness is fairly high, while in sporadic patients is low. Screening for mitochondrial 1555(G) mutation is of potential value to clinical use.
Yuan
H
H
Institute of Otolaryngology,PLA General Hospital, Beijing, 100853 P. R. China. yuanhj@plagh.com.cn
Jiang
S
S
Yang
W
W
Guo
W
W
Cao
J
J
Dai
P
P
D000293:Adolescent; D000328:Adult; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D002648:Child; D002675:Child, Preschool; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000139:chemically induced*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009154:Mutation; D010375:Pedigree
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial