title: Glucosamine content of tempe mould, Rhizopus oligosporus.
abstract: The glucosamine content of Rhizopus oligosporus NRRL 2710 mycelium grown in different media was determined. In Sabouraud dextrose broth the glucosamine content ranged from 51 g (kg dry biomass)(-1) for mycelial pellets less than 5 mm diameter to 107 g kg(-1) for pellets 16-35 mm diameter. Mycelium grown on Sabouraud dextrose agar contained 111 g glucosamine (kg dry biomass)(-1) while that grown on soymilk agar, used to simulate growth on soybeans in tempe, contained 82 g kg(-1). The estimation of glucosamine was reproducible, with a mean coefficient of variation of 4% for mycelial pellets and 11% for mycelium from agar media. It is suggested that a conversion factor of 12 g dry biomass (g glucosamine)(-1) is applicable to determine fungal biomass in tempe fermentation.
authors.affiliation: Department of Food Science and Technology, The University of Reading, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Sparringa
authors.forename: J D
authors.initials: JD
authors.lastname: Owens
mesh_terms: D018533:Biomass; D003470:Culture Media; D005944:Glucosamine / Q000032:analysis*; D012233:Rhizopus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D013025:Glycine max / Q000382:microbiology*; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D003470:Culture Media; D005944:Glucosamine