title: Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms.
abstract: The enzymatic formation of glycosidic bonds may be catalyzed by the transfer of the glycosyl moiety from an activated nucleotide-diphospho-sugar donor to a specific acceptor. SpsA is a glycosyltransferase implicated in the synthesis of the spore coat of Bacillus subtilis, whose homologues include cellulose synthase and many lipopolysaccharide and bacterial O-antigen synthases. The three-dimensional crystal structure of SpsA has been determined by conventional MIR techniques at a resolution of 1.5 A. It is a two-domain protein with a nucleotide-binding domain together with an acceptor binding domain which features a disordered loop spanning the active site. The structures of SpsA in complex with both Mg-UDP and Mn-UDP have also been determined at 2.0 and 1.7 A, respectively. These complexes, together with the sequence conservation, begin to shed light on the mechanism of this ubiquitous family of inverting glycosyltransferases.
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Charnock
authors.forename: G J
authors.initials: GJ
authors.lastname: Davies
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002482:Cellulose / Q000096:biosynthesis; D002686:Chitin / Q000096:biosynthesis; D003198:Computer Simulation; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009702:Nucleoside Diphosphate Sugars / Q000737:chemistry* / Q000378:metabolism; D012984:Software; D014530:Uridine Diphosphate / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009702:Nucleoside Diphosphate Sugars; C110344:SpsA protein, Streptococcus pneumoniae; D002686:Chitin; D014530:Uridine Diphosphate; D002482:Cellulose; D016695:Glycosyltransferases