title: The primer generator: a program that facilitates the selection of oligonucleotides for site-directed mutagenesis.
abstract: Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins. Newer PCR-based mutagenesis techniques have reduced the process of mutagenesis to as little as one day. While each technique has its advantages, both require a strategy to isolate the desired clone from a population that contains mutagenized and wild-type genes. In this report, we describe a World Wide Web-based computer program that facilitates the design of mutagenic primers such that successfully mutagenized clones can be identified by the presence or absence of a unique restriction site.
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: A
authors.initials: A
authors.lastname: Turchin
authors.forename: J F
authors.initials: JF
authors.lastname: Lawler
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D017931:DNA Primers / Q000138:chemical synthesis* / Q000235:genetics*; D004262:DNA Restriction Enzymes; D015195:Drug Design; D020407:Internet; D016297:Mutagenesis, Site-Directed; D012984:Software; D012985:Software Design
chemical_list: D017931:DNA Primers; D004262:DNA Restriction Enzymes