Effects of larval mosquitoes (Aedes triseriatus) and stemflow on microbial community dynamics in container habitats.
The dynamics of the microbial food sources for Aedes triseriatus larvae in microcosms were found to be strongly influenced by larval presence. The total abundance of bacteria in water samples generally increased in response to larvae, including populations of cultivable, facultatively anaerobic bacteria. Additionally, a portion of the community shifted from Pseudomonaceae to Enterobacteriaceae. Bacterial abundance on leaf material was significantly reduced in the presence of actively feeding larvae. Principle-component analysis of whole community fatty acid methyl ester (FAME) profiles showed that larvae changed the microbial community structure in both the water column and the leaf material. Cyclopropyl FAMEs, typically associated with bacteria, were reduced in microcosms containing larvae; however, other bacterial fatty acids showed no consistent response. Long-chain polyunsaturated fatty acids characteristic of microeukaryotes (protozoans and meiofauna) declined in abundance when larvae were present, indicating that larval feeding reduced the densities of these microorganisms. However, presumed fungal lipid markers either increased or were unchanged in response to larvae. Larval presence also affected microbial nitrogen metabolism through modification of the physiochemical conditions or by grazing on populations of bacteria involved in nitrification-denitrification. Stemflow primarily influenced inorganic ion and organic compound concentrations in the microcosms and had less-pronounced effects on microbial community parameters than did larval presence. Stemflow treatments diluted concentrations of all inorganic ions (chloride, sulfate, and ammonium) and organic compounds (total dissolved organic carbon, soluble carbohydrates, and total protein) measured, with the exceptions of nitrite and nitrate. Stemflow addition did not measurably affect larval biomass in the microcosms but did enhance development rates and early emergence patterns of adults.
Kaufman
M G
MG
W. K. Kellogg Biological Station, Michigan State University, Hickory Corners, Michigan 49060, USA. kaufman@kbs.msu.edu
Walker
E D
ED
Smith
T W
TW
Merritt
R W
RW
Klug
M J
MJ
D000330:Aedes / Q000254:growth & development*; D000818:Animals; D000838:Anions / Q000032:analysis; D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D015169:Colony Count, Microbial; D017753:Ecosystem; D005227:Fatty Acids / Q000032:analysis; D005247:Feeding Behavior; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D007814:Larva / Q000254:growth & development; D018515:Plant Leaves / Q000382:microbiology*; D014197:Trees; D014871:Water Microbiology
D000838:Anions; D005227:Fatty Acids