[The application of the polymerase chain reaction technic for the detection of human papillomavirus sequences].
The polymerase chain reaction technique was applied to detect sequences of human Papillomavirus (HPV) by controls of cellular lines of cervical cancer and of tissues obtained through biopsy with a HPV-positive clinical diagnosis. A set of consensus oligonucleotides, which are complementary to a highly conserved region within the open reading frame E1 of the viral genome of HPV affecting the cervical mucosa, was used. With these primers it was possible to amplify DNA sequences corresponding to HPV 6 and 11, considered in the low risk group, and to HPV 16, 18, 31 and 33, included in the high risk group. The study of the sensitivity of the amplification technique showed a level of detection of 3,5 viral particles per each cellular diploid genome.
Soto
Y
Y
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Muné
M
M
Goicolea
A
A
Morales
E
E
Santoyo
J M
JM
Valdés
O
O
Ramírez
R
R
Pimentel
T
T
D004279:DNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D006801:Humans; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation / Q000379:methods
D004279:DNA, Viral; D015345:Oligonucleotide Probes