Inducers of nod genes of Rhizobium ciceri.
Induction of nodABC genes of R. ciceri was studied by constructing nodABC-lacZ fusion. The root exudates of the homologous hosts induced the expression of nodABC genes but those of heterologous hosts failed to do so. The HPLC analysis of the root exudates of C. arietinum showed the presence of 6-7 compounds with retention times matching to flavonoids like naringenin, hesperetin, daidzein, naringin, 7 OH coumarin and luteolin. Induction studies using the standard flavonoids showed naringenin, followed by daidzein, as most potent inducer of the nodABC genes of R. ciceri. Naringenin in combination with daidzein showed a synergistic effect on the expression of nodABC genes.
Srivastava
P
P
Department of Microbiology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar, India. ncr75@hotmail.com
Sharma
P K
PK
Dogra
R C
RC
D019677:Artificial Gene Fusion / Q000379:methods; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005798:Genes, Bacterial / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology; D007763:Lac Operon / Q000187:drug effects / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics*; D014157:Transcription Factors / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D044950:Flavanones; D005419:Flavonoids; D007529:Isoflavones; D014157:Transcription Factors; C004742:daidzein; C005273:naringenin