Identification of bacteria using two degenerate 16S rDNA sequencing primers.
Two degenerate 16S rDNA primers have been designed for broad-range identification of eubacteria by PCR and automated sequencing. Using a simple method, the primers have proven useful in identification of proteobacteria (Campylobacter, Enterobacter, Escherichia, Helicobacter, Klebsiella), gram-positive bacteria (Mycobacterium, Staphylococcus, Streptococcus) and spirochetes (Borrelia) derived from clinical samples. In several cases, the samples could be identified at the species level.
Boye
K
K
Clinical Biochemistry Department, Statens Serum Institut, Copenhagen, Denmark. kbo@ssi.dk
Høgdall
E
E
Borre
M
M
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D001898:Borrelia / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D013045:Species Specificity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S