Characterization of Drosophila Presenilin and its colocalization with Notch during development.
Mutant Presenilin proteins cause early-onset familial Alzheimer's disease in humans and Caenorhabditis elegans Presenilins may facilitate Notch receptor signaling. We have isolated a Drosophila Presenilin homologue and determined the spatial and temporal distribution of the encoded protein as well as its localization relative to the fly Notch protein. In contrast to previous mRNA in situ studies, we find that Presenilin is widely expressed throughout oogenesis, embryogenesis, and imaginal development, and generally accumulates at comparable levels in neuronal and nonneuronal tissues. Double immunolabeling with Notch antibodies revealed that Presenilin and Notch are coexpressed in many tissues throughout Drosophila development and display partially overlapping subcellular localizations, supporting a possible functional link between Presenilin and Notch.
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Department of Genetics, University of Pennsylvania School of Medicine, Stellar-Chance Laboratories 709C, Philadelphia 19104, USA.
Fortini
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D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D004330:Drosophila / Q000196:embryology* / Q000235:genetics / Q000254:growth & development; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics; D053763:Presenilins; D051880:Receptors, Notch; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions
D000906:Antibodies; D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D053763:Presenilins; C106427:Psn protein, Drosophila; D051880:Receptors, Notch