Saccharomyces cerevisiae cells harboring the gene encoding sarcotoxin IA secrete a peptide that is toxic to plant pathogenic bacteria.
Sarcotoxin IA is a cecropin-type antibacterial protein produced by the flesh fly, Sarcophaga peregrina. Similar to other bactericidal small proteins produced by insects, sarcotoxin IA is released into the hemolymph of larvae and nymphs upon mechanical injury or bacterial infection. The gene (sarco) that encodes this toxin was introduced into Saccharomyces cerevisiae yeast cells and was expressed under a constitutive yeast promoter. The transformed yeast cells were grown in a liquid medium, and a peptide with a similar molecular size to that of the mature sarcotoxin IA was detected in the medium by Western blot analysis. The secreted sarcotoxin-like peptide (SLP) had a potent cytotoxic effect against several bacteria, including plant pathogenic bacteria, similar to the toxic effects of the authentic sarcotoxin IA. Erwinia carotovora was more susceptible to the toxic medium than Pseudomonas solanacearum and Pseudomonas syringae pv. lachrymans. Thus, yeast may be used in the production of such proteins for employment against various bacterial pathogens.
Aly
R
R
Department of Weed Research, A.R.O., Newe Ya'ar Research Center, Ramat Yishay, 30095, Israel. weedres@netvision.net.il
Granot
D
D
Mahler-Slasky
Y
Y
Halpern
N
N
Nir
D
D
Galun
E
E
D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology; D004175:Diptera / Q000235:genetics; D017344:Genes, Insect; D019476:Insect Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D016973:Pectobacterium carotovorum / Q000187:drug effects; D010944:Plants / Q000382:microbiology; D011549:Pseudomonas / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D019476:Insect Proteins; D011994:Recombinant Proteins; C054733:sarcotoxin IA protein, Sarcophaga peregrina