title: Genomic structure, tissue expression and chromosomal location of the LIM-only gene, SLIM1.
abstract: Human SLIM1 is a recently described gene of the LIM-only class encoding four and a half tandemly repeated LIM domains. LIM domains are double zinc finger structures which provide an interface for protein/protein interactions and are conserved in a variety of nuclear and cytoplasmic factors important in cell fate determination and cellular regulation. Here we report the structural organization, expression pattern and chromosomal localization of the human SLIM1 gene. SLIM1 was found to contain at least five exons with all four introns disrupting the coding region at a similar position relative to the respective complete LIM domains. Northern blot analysis confirmed strikingly high expression of SLIM1 in skeletal muscle and heart, with much lower expression observed in several other tissues including colon, small intestine and prostate. The SLIM1 gene was assigned to human chromosome Xq26 using fluorescence in situ hybridization.
authors.affiliation: TVW Telethon Institute for Child Health Research, Division of Childrens' Leukaemia and Cancer Research, West Perth, WA 6872, Australia. greenew@numbat.murdoc.edu.au
authors.forename: W K
authors.initials: WK
authors.lastname: Greene
authors.forename: E
authors.initials: E
authors.lastname: Baker
authors.forename: T H
authors.initials: TH
authors.lastname: Rabbitts
authors.forename: U R
authors.initials: UR
authors.lastname: Kees
mesh_terms: D002874:Chromosome Mapping; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D007438:Introns; D060588:LIM Domain Proteins; D009124:Muscle Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D014960:X Chromosome / Q000235:genetics
chemical_list: C101149:FHL1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009124:Muscle Proteins