title: Generation of epitope-tagged proteins by inverse PCR mutagenesis.
authors.affiliation: Johns Hopkins University School of Medicine, Department of Physiology, Baltimore, MD 21205, USA.
authors.forename: L
authors.initials: L
authors.lastname: Gama
authors.forename: G E
authors.initials: GE
authors.lastname: Breitwieser
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D017931:DNA Primers / Q000235:genetics; D000939:Epitopes / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides; D011506:Proteins; C085706:FLAG peptide