Temporal and spatial expressions of transforming growth factor-betas and their receptors in epiphyseal growth plate.
The activities of TGF-beta and its receptors in enchondral ossification have not yet been fully elucidated. The temporal and spatial expressions of TGF-beta and its receptors were examined immunohistochemically in epiphyseal growth plate of rats, in order to clarify the roles of TGF-beta and receptors in cartilage cells during enchondral ossification. TGF-beta1 and TGF-beta3 were expressed from 6 to 24 weeks in resting, proliferating, and maturing zone. TGF-beta2 was expressed at 6 week, but decreased during growth. The expression of TGF-betas in hypertrophic chondrocytes was weak. TbetaR-I was co-expressed with the ligand in resting, proliferating, and maturing zone throughout the development. The expressions of TbetaR-II was recognized at 6 weeks but decreased after that. This study suggests that TGF-betas play a major role in modulating endochondral ossification during growth in epiphyseal growth plate.
Matsunaga
S
S
Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
Yamamoto
T
T
Fukumura
K
K
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D006132:Growth Plate / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis / Q000502:physiology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000502:physiology
D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta