title: A novel 45 kDa secretory protein from rat olfactory epithelium: primary structure and localisation.
abstract: cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein and the proteins involved in the transfer of hydrophobic ligands. Using in situ hybridisation, the 45 kDa protein mRNA expression was detected in the layer of supportive cells of olfactory epithelium, apical region of trachea, surface layer of the ciliated bronchial epithelium in lung and in skin epidermis.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: M I
authors.initials: MI
authors.lastname: Merkulova
authors.forename: S G
authors.initials: SG
authors.lastname: Andreeva
authors.forename: T M
authors.initials: TM
authors.lastname: Shuvaeva
authors.forename: S V
authors.initials: SV
authors.lastname: Novoselov
authors.forename: I V
authors.initials: IV
authors.lastname: Peshenko
authors.forename: M F
authors.initials: MF
authors.lastname: Bystrova
authors.forename: V I
authors.initials: VI
authors.lastname: Novoselov
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
authors.forename: V M
authors.initials: VM
authors.lastname: Lipkin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009831:Olfactory Mucosa / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger