title: Release of outer membrane vesicles from Bordetella pertussis.
abstract: The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly- showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.
authors.affiliation: Centro de Investigación y Desarrollo en Fermentaciones Industriales, CONICET Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
authors.forename: D
authors.initials: D
authors.lastname: Hozbor
authors.forename: M E
authors.initials: ME
authors.lastname: Rodriguez
authors.forename: J
authors.initials: J
authors.lastname: Fernández
authors.forename: A
authors.initials: A
authors.lastname: Lagares
authors.forename: N
authors.initials: N
authors.lastname: Guiso
authors.forename: O
authors.initials: O
authors.lastname: Yantorno
mesh_terms: D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins / Q000032:analysis; D015153:Blotting, Western; D001886:Bordetella pertussis / Q000648:ultrastructure*; D018699:Coated Vesicles / Q000737:chemistry / Q000378:metabolism*; D006388:Hemagglutinins / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis; D008854:Microscopy, Electron; D011498:Protein Precursors / Q000032:analysis; D010566:Virulence Factors, Bordetella / Q000032:analysis
chemical_list: D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D006388:Hemagglutinins; D008070:Lipopolysaccharides; D011498:Protein Precursors; D010566:Virulence Factors, Bordetella