title: Lacticin 3147 displays activity in buffer against gram-positive bacterial pathogens which appear insensitive in standard plate assays.
abstract: Lacticin 3147 is a broad-spectrum bacteriocin produced by Lactococcus lactis subsp. lactis DPC3147, which has been shown to be active against a range of food-borne bacteria. The reported inhibitory range for lacticin is extended to include methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecalis, penicillin-resistant Pneumococcus, Propionibacterium acne and Streptococcus mutans. This extended host range is not obvious from traditional agar plate-based methods, but reductions in bacterial cell numbers by up to 6 log10 cfu ml-1 was observed after 2 h in time-kill curve studies conducted in broth, suggesting that the bacteriocin may have potential as a therapeutic agent in the treatment of human infections.
authors.affiliation: National Food Biotechnology Centre, University College Cork, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Galvin
authors.forename: C
authors.initials: C
authors.lastname: Hill
authors.forename: R P
authors.initials: RP
authors.lastname: Ross
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology; D001430:Bacteriocins / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000472:pathogenicity*; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; C082508:lacticin 481