title: Highly specific detection of H2O2-dependent luminol chemiluminescence in stimulated human leukocytes using polyvinyl films.
abstract: When human polymorphonuclear leukocytes (PMN) were attached to glass coverslips, cells always spread and formed reactive oxygen species prior to any experimental stimulation. To avoid this, a polyvinylidine chloride film was used as an inactive substance to place the cells. Cells engaged in phagocytosis on the film exhibited a specific H2O2-mediated luminol chemiluminescence (LCL) at the cell-particle interface; the cells stimulated with 12-O-tetradecanoylphorbol-13-acetate became aggregated and the LCL was observed at the cell-cell contact. These results corresponded well with those obtained by an electron microscopic H2O2-demonstration method.
authors.affiliation: Department of Anatomy, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan. keiichi@dpc.aichi-gakuin.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Moriguchi
authors.forename: N
authors.initials: N
authors.lastname: Ohno
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism*; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis; D011145:Polyvinyls; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D011145:Polyvinyls; D017382:Reactive Oxygen Species; D008165:Luminol; D006861:Hydrogen Peroxide