Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli.
To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solutions containing at least 0.25 g dry cell weight/L has been developed. The detection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metabolism, extraction of nucleotides, and quantitative analysis by ion-pair reversed-phase HPLC.
Meyer
S
S
Institut für Biotechnologie, Universität Halle, Kurt-Mothes-Strasse 3, Halle, D-06120, Germany.
Noisommit-Rizzi
N
N
Reuss
M
M
Neubauer
P
P
D000227:Adenine Nucleotides / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004734:Energy Metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006150:Guanine Nucleotides / Q000032:analysis* / Q000378:metabolism
D000227:Adenine Nucleotides; D006150:Guanine Nucleotides