title: Comparison of basophil histamine releasability between atopic and nonatopic asthmatics.
abstract: To compare the mediator releasability between atopic and nonatopic asthmatics, we measured basophil histamine releasability (BaHR) using a calcium-ionophore A23187 and anti-IgE in 137 subjects who were treated at Seoul National University Hospital. Subjects were categorized into atopic (group AA, n=77) or nonatopic asthmatics (group NA, n=32), or normal controls (group NC, n=28). Serum total IgE levels were determined and correlation with BaHR was assessed. Anti-IgE-induced maximal BaHR in groups AA, NA, and NC was 41.0+/-3.2, 23.1+/-4.5, and 16.8+/-3.8, respectively (mean+/-SE, %). Anti-IgE-induced BaHR in group AA was significantly higher than that in groups NA and NC (p<0.05). Calcium ionophore A23187-induced maximal BaHR was 43.1+/-2.8, 40.8+/-4.4, and 50.5+/-5.2, respectively (mean+/-SE, %), and there was no significant difference among the groups. Serum total IgE level correlated significantly with anti-IgE-induced maximal BaHR (r=0.281, p<0.01) but not with that induced by calcium ionophore A23187. In conclusion, IgE receptor-related BaHR is higher in atopic asthmatics than in nonatopic asthmatics, and this increased BaHR in atopics is significantly associated with increased serum total IgE level.
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Medical Research Center, Korea.
authors.forename: J W
authors.initials: JW
authors.lastname: Son
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Chung
authors.forename: S R
authors.initials: SR
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Cho
authors.forename: K U
authors.initials: KU
authors.lastname: Min
authors.forename: Y Y
authors.initials: YY
authors.lastname: Koh
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kim
mesh_terms: D001249:Asthma / Q000276:immunology*; D001491:Basophils / Q000187:drug effects / Q000276:immunology*; D000001:Calcimycin / Q000494:pharmacology; D002648:Child; D006636:Histamine Release / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007476:Ionophores / Q000494:pharmacology
chemical_list: D007476:Ionophores; D007073:Immunoglobulin E; D000001:Calcimycin