title: Glutathione depletion causes an uncoupling effect on retinal horizontal cells through oxidative stress.
abstract: To investigate a physiological role of glutathione in the horizontal cells of carp retina, the gap junctional intercellular communication between horizontal cells was studied using the techniques of intracellular recording of light-induced responses and coupling of the fluorescence dye Lucifer Yellow. Intravitreal injection of 2.5 micromol L-buthionine sulfoximine, an inhibitor of glutathione synthesis, induced a dramatic reduction (20% of control) of retinal glutathione level two days after treatment. The low level of glutathione continued for a further four to five days, and thereafter gradually recovered to about 40% (20 days after injection) and 70% (50 days after injection) of the control level. The spatial properties of the photopic L-type horizontal cell response were examined by enlarging the diameter of the central spot and peripheral annulus over the recording point. In normal retinas, the response amplitude of horizontal cells was monotonically enhanced as the diameter of the spot increased (0.5-4.0 mm) and correspondingly the dye diffusion area was wide, as the injected Lucifer Yellow normally diffused to several neighboring cells. Treatment with L-buthionine sulfoximine significantly altered the spatial properties of horizontal cells by increasing the response amplitude to central spots and slightly decreasing that to peripheral annuli, which were observed by four days after injection. It also restricted intracellular Lucifer Yellow to one or two cells. Accompanying the recovery of the cellular level of glutathione, the spatial properties and dye coupling of horizontal cells were restored to normal. A time lag (two days) of initiation in retinal glutathione depletion and alteration of spatial or dye coupling properties of horizontal cells is discussed, together with reactive oxygen species accumulation.
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medicine, University of Kanazawa, Japan.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Ohkawa
authors.forename: K
authors.initials: K
authors.lastname: Muramoto
authors.forename: K
authors.initials: K
authors.lastname: Homma
authors.forename: K
authors.initials: K
authors.lastname: Mawatari
authors.forename: M
authors.initials: M
authors.lastname: Devadas
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002347:Carps; D002450:Cell Communication / Q000502:physiology; D004058:Diffusion; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D005978:Glutathione / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000378:metabolism; D007546:Isoquinolines / Q000493:pharmacokinetics; D018384:Oxidative Stress / Q000502:physiology*; D012160:Retina / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; D019328:Buthionine Sulfoximine; C017475:lucifer yellow; D005978:Glutathione