title: Broadly protective vaccine for Staphylococcus aureus based on an in vivo-expressed antigen.
abstract: Vaccines based on preferential expression of bacterial antigens during human infection have not been described. Staphylococcus aureus synthesized poly-N-succinyl beta-1-6 glucosamine (PNSG) as a surface polysaccharide during human and animal infection, but few strains expressed PNSG in vitro. All S. aureus strains examined carried genes for PNSG synthesis. Immunization protected mice against kidney infections and death from strains that produced little PNSG in vitro. Nonimmune infected animals made antibody to PNSG, but serial in vitro cultures of kidney isolates yielded mostly cells that did not produce PNSG. PNSG is a candidate for use in a vaccine to protect against S. aureus infection.
authors.affiliation: Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Department of Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: D
authors.initials: D
authors.lastname: McKenney
authors.forename: K L
authors.initials: KL
authors.lastname: Pouliot
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: V
authors.initials: V
authors.lastname: Murthy
authors.forename: M
authors.initials: M
authors.lastname: Ulrich
authors.forename: G
authors.initials: G
authors.lastname: Döring
authors.forename: J C
authors.initials: JC
authors.lastname: Lee
authors.forename: D A
authors.initials: DA
authors.lastname: Goldmann
authors.forename: G B
authors.initials: GB
authors.lastname: Pier
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D016667:Bacterial Capsules / Q000276:immunology; D002648:Child; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007668:Kidney / Q000276:immunology / Q000382:microbiology; D007674:Kidney Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D011817:Rabbits; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000276:immunology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D013209:Staphylococcal Vaccines; C119521:poly-N-succinyl beta-1-6-glucosamine