[The intratypic characterization of the poliovirus by the polymerase chain reaction technic].
The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about the non circulation of the wild poliovirus in Cuba.
Avalos Redón
I
I
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Más Lago
P J
PJ
Sarmiento Pérez
L R
LR
Palomera Puente
R
R
Muné Jiménez
M
M
Bello Corredor
M
M
Pérez Santos
L
L
D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D017955:Poliovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012367:RNA, Viral / Q000235:genetics
D015345:Oligonucleotide Probes; D012367:RNA, Viral