title: Labeling peptides with rhenium-188.
abstract: A direct labeling technique via EHDP for the preparation of 188Re-somatostatin analogue peptide beta-(2-naphthyl)-D-Ala-Cys-Tyr-D-Trp-Lys-Val-Cys-Thr-amide complex was developed. The influence of reaction conditions such as pH, temperature, weak ligand concentration and stannous chloride concentration were investigated. Methods of analysis were also established permitting identification of radiochemical impurities which may be present in the radiopharmaceutical solution. Results showed that under the procedure reported herein 188Re-peptide complex can be prepared with a radiochemical purity of 90% and a specific activity up to 1.8 GBq mg-1 without radiolytic degradation of the product.
authors.affiliation: Instituto Nacional de Nutrición, Salvador Zubirán, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Meléndez-Alafort
authors.forename: G
authors.initials: G
authors.lastname: Ferro-Flores
authors.forename: C
authors.initials: C
authors.lastname: Arteaga-Murphy
authors.forename: M
authors.initials: M
authors.lastname: Pedraza-López
authors.forename: M A
authors.initials: MA
authors.lastname: González-Zavala
authors.forename: J I
authors.initials: JI
authors.lastname: Tendilla
authors.forename: L
authors.initials: L
authors.lastname: García-Salinas
mesh_terms: D007553:Isotope Labeling; D012211:Rhenium; D013004:Somatostatin
chemical_list: D013004:Somatostatin; D012211:Rhenium