title: Chlamydia pneumoniae reactive T lymphocytes in the walls of abdominal aortic aneurysms.
abstract: BACKGROUND
  The presence of Chlamydia pneumoniae in the walls of abdominal aortic aneurysms (AAAs) has been demonstrated recently, but its role in the cause and/or maintenance of aortic wall inflammation is not known. In the present study, we have investigated the possible relationship between C. pneumoniae and the antigen specificity of T lymphocytes mediating inflammation in AAA tissue.
  
  
  METHODS
  Tissue specimens were obtained from 22 consecutive AAA patients undergoing elective surgery (mean age 67 +/- 1 year). Immunohistochemical analysis of the formalin-fixed tissue was performed using the streptavidin-biotin-peroxidase method. In vivo activated T lymphocytes were propagated from the specimens with interleukin (IL) 2, and antigen specificity of the established T-cell lines was analysed in the presence of autologous antigen-presenting cells using radioactive thymidine labelling.
  
  
  RESULTS
  Immunohistological staining of AAA tissue showed the presence of C. pneumoniae antigen in 55% (6/11) of the samples studied. The inflammatory cell infiltrate of the AAA tissue contained 60-90% T (CD45RO) and 0-10% B (CD20) cells. When the tissue specimens were cultured without antigen in the presence of IL-2, lymphocyte propagation was achieved in 17 out of the 22 samples. Chlamydia pneumoniae antigen was found to induce a positive proliferative response in 8 of the 17 lines.
  
  
  CONCLUSIONS
  The presence of C. pneumoniae specific T lymphocytes among in vivo activated cells from the AAA tissue specimens suggests that C. pneumoniae participates in the maintenance of the inflammatory response in the tissue and may thus be involved in the progression of the disease.
authors.affiliation: National Public Health Institute, Oulu, University of Oulu, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Halme
authors.forename: T
authors.initials: T
authors.lastname: Juvonen
authors.forename: A
authors.initials: A
authors.lastname: Laurila
authors.forename: J
authors.initials: J
authors.lastname: Juvonen
authors.forename: M
authors.initials: M
authors.lastname: Mosorin
authors.forename: P
authors.initials: P
authors.lastname: Saikku
authors.forename: H M
authors.initials: HM
authors.lastname: Surcel
mesh_terms: D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D001012:Aorta, Abdominal / Q000276:immunology* / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D016993:Chlamydophila pneumoniae / Q000276:immunology* / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G