Early embryonic expression of the growth hormone family protein genes in the developing rainbow trout, Oncorhynchus mykiss.
In fish, growth hormone (GH), prolactin (PRL) and somatolactin (SL) are three major peptide hormones produced in the pituitary gland. Using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis of the amplified products, the expression of GH, PRL, and SL genes were determined during the embryonic development in rainbow trout (Oncorhynchus mykiss). The mRNA for GH, PRL and SL were detected not only in embryos prior to or after organogenesis of the pituitary gland but also in mature oocytes as maternal messages. After hatching, all of these three mRNA species were detected at very high levels. Since the ontogenesis of the pituitary gland takes place on embryonic day (ED) 14, these observations suggest that the GH family protein genes are expressed in the developing embryos prior to the formation of the pituitary gland. Using the same RT-PCR assay, PRL mRNA was detected only in the head part of the fry whereas GH and SL mRNA were detected in both the head and trunk. In adult animals, though high levels of GH mRNA were primarily detected in the pituitary gland, brain, gill, and heart, low levels of GH mRNA were also detected in the kidney, liver, pyloric ceaca, and ovary. Results of the current study and those reported by Yang et al. (1997. Gen Comp Endocrinol 106:271-281) suggest that GH and SL genes are also expressed in extrapituitary tissues even after the organogenesis of the pituitary gland. Furthermore, these results suggest that these hormones may play important roles yet to be identified during embryonic development in fish.
Yang
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BY
Biotechnology Center and Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-4156, USA.
Greene
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M
Chen
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TT
D000818:Animals; D015139:Blotting, Southern; D017931:DNA Primers; D015342:DNA Probes; D005260:Female; D029941:Fish Proteins; D005784:Gene Amplification; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000235:genetics*; D010902:Pituitary Gland; D010907:Pituitary Hormones / Q000235:genetics*; D011388:Prolactin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D015342:DNA Probes; D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone