Interaction between RGS7 and polycystin.
Regulators of G protein signaling (RGS) proteins accelerate the intrinsic GTPase activity of certain Galpha subunits and thereby modulate a number of G protein-dependent signaling cascades. Currently, little is known about the regulation of RGS proteins themselves. We identified a short-lived RGS protein, RGS7, that is rapidly degraded through the proteasome pathway. The degradation of RGS7 is inhibited by interaction with a C-terminal domain of polycystin, the protein encoded by PKD1, a gene involved in autosomal-dominant polycystic kidney disease. Furthermore, membranous expression of C-terminal polycystin relocalized RGS7. Our results indicate that rapid degradation and interaction with integral membrane proteins are potential means of regulating RGS proteins.
Kim
E
E
Laboratory of Molecular and Developmental Neuroscience, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Arnould
T
T
Sellin
L
L
Benzing
T
T
Comella
N
N
Kocher
O
O
Tsiokas
L
L
Sukhatme
V P
VP
Walz
G
G
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000378:metabolism; D001665:Binding Sites; D003546:Cysteine Endopeptidases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020710:RGS Proteins; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D050396:TRPP Cation Channels; D014158:Transcription, Genetic; D014452:Ubiquitins / Q000378:metabolism
D003546:Cysteine Endopeptidases; D019204:GTP-Binding Proteins; D009097:Multienzyme Complexes; D046988:Proteasome Endopeptidase Complex; D011506:Proteins; D020710:RGS Proteins; D011994:Recombinant Proteins; D050396:TRPP Cation Channels; D014452:Ubiquitins; C492039:RGS7 protein, human