Proinflammatory cytokines secreted by monocytes of filarial patients.
The levels of interleukin 1, granulocyte macrophage colony stimulating factor (GM-CSF) and tumour necrosis factor (TNF)-alpha secreted by the monocytes of filarial patients, such as asymptomatic microfilaremics (MF), chronic pathology (CP), and normal individuals, residing in a Wuchereria bancrofti endemic area (EN) in response to whole Brugia malayi antigen (BmA) and Setaria digitata (Sd-cuticular) and a recombinant filarial antigen (pRJ51) were studied. Stimulation of peripheral blood adherent cells with whole parasite antigen showed marked increase in IL-1 levels in MF as compared to CP or EN. The recombinant antigen stimulation, however, resulted in similar levels of IL-1 in MF and CP. In contrast, stimulation of peripheral blood adherent cells with whole parasite antigen produced high levels of GM-CSF and TNF-alpha in CP as opposed to MF or EN. Recombinant antigen stimulation, however, produced high levels of GM-CSF in EN as compared to MF or CP, while no significant change in the release of TNF-alpha was observed in these patients. These results suggest that monocytes from filarial patients exhibit functional activity similar to that observed by the monocytes of endemic normals (control group).
Raman
U
U
Centre for Biotechnology, Anna University, Chennai, Nadu, India.
Eswaran
D
D
Narayanan
R B
RB
Jayaraman
K
K
Kaliraj
P
P
D000818:Animals; D000947:Antigens, Helminth; D017178:Brugia malayi / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D004605:Elephantiasis, Filarial / Q000276:immunology*; D006801:Humans; D008842:Microfilariae / Q000276:immunology*; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins; D017182:Setaria Nematode / Q000276:immunology
D000947:Antigens, Helminth; D016207:Cytokines; D011994:Recombinant Proteins