[Cloning the 5 ' end fragment of ST13 cDNA by nested PCR].
OBJECTIVE
To clone and sequence the 5' -end fragment of ST13 cDNA.


METHODS
The 5' -end fragment of the ST13cDNA was amplified directly from cDNA library by nested PCR method, and cloned into pGEM-T. easy vector, then the sequencing of the inserted PCR product was performed.


RESULTS
After primary and secondary PCR, two PCR products obtained with the size of about 550bp and 480bp, respectively, were both proved to be the 5' -end of ST13 cDNA by sequencing.


CONCLUSIONS
the method presented is very simple and effective for cloning and sequencing the 5'-end of a target gene.
Geng
L
L
Cancer Institute of Zhejiang Medical University, Hangzhou, 310009 P.R. China. jcao@public.hz.zj.cn
Cai
X
X
Cao
J
J
Fang
Y
Y
D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*