title: Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori in patients with different clinical presentations.
abstract: Chronic Helicobacter pylori infection is associated with mucosal inflammation. The aim of the present study was to assess human neutrophil and monocyte activation by H. pylori strains obtained from patients with different clinical presentations. Bacterial sonicates from 12 strains were used to stimulate phagocyte upregulation of CD11b/CD18 adherence molecules assessed by fluorescence flow cytometry and oxidative burst responses assessed by chemiluminescence. A dose-dependent activation of CD11b/CD18 adherence molecules was observed with all strains on both neutrophils and monocytes. The activities were similar for strains from patients with duodenal ulceration and for strains from asymptomatic volunteers irrespective of histopathologic grades of the biopsy specimens from the antral mucosa. The neutrophil chemiluminescence response correlated with histopathologic severity. We conclude that upregulation of neutrophil and monocyte adherence molecules by H. pylori sonicates is not associated with clinical presentation of the infection.
authors.affiliation: Department of Clinical Immunology, Aalborg Hospital, Aalborg, Denmark.
authors.forename: P S
authors.initials: PS
authors.lastname: Hansen
authors.forename: M F
authors.initials: MF
authors.lastname: Go
authors.forename: K
authors.initials: K
authors.lastname: Varming
authors.forename: L P
authors.initials: LP
authors.lastname: Andersen
authors.forename: R M
authors.initials: RM
authors.lastname: Genta
authors.forename: D Y
authors.initials: DY
authors.lastname: Graham
authors.forename: H
authors.initials: H
authors.lastname: Nielsen
mesh_terms: D018821:CD18 Antigens / Q000276:immunology; D004381:Duodenal Ulcer / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D016481:Helicobacter Infections / Q000097:blood / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D016177:Macrophage-1 Antigen / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D018375:Neutrophil Activation / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*
chemical_list: D018821:CD18 Antigens; D016177:Macrophage-1 Antigen