title: Mechanism of alanine hyperproduction by arthrobacter oxydans HAP-1: metabolic shift to fermentation under nongrowth aerobic conditions
abstract: Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We propose that this enzyme functions as an electron sink even under aerobic conditions.
authors.affiliation: Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan.
authors.initials: Si
authors.lastname: Hashimoto
authors.initials: R
authors.lastname: Katsumata