Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase.
Transient-state kinetic experiments and analyses have been performed to examine the validity of hitherto unchallenged evidence proposed to be indicative of a channelled transfer of triose phosphates from aldolase to glyceraldehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behaviour of the examined aldolase-dehydrogenase reactions is fully consistent with a free-diffusion mechanism of metabolite transfer.
Pettersson
H
H
Avdelninger för Biokemi, Kemicentrum, Lunds Universitet, Lund, Sweden.
Pettersson
G
G
D002384:Catalysis; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry*; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry; D007554:Isotopes; D007700:Kinetics
D007554:Isotopes; D005993:Glycerolphosphate Dehydrogenase; D005634:Fructose-Bisphosphate Aldolase