Applications of the green fluorescent protein as a molecular marker in environmental microorganisms.
In this review, we examine numerous applications of the green fluorescent protein (GFP) marker gene in environmental microbiology research. The GFP and its variants are reviewed and applications in plant-microbe interactions, biofilms, biodegradation, bacterial-protozoan interactions, gene transfer, and biosensors are discussed. Methods for detecting GFP-marked cells are also examined. The GFP is a useful marker in environmental microorganisms, allowing new research that will increase our understanding of microorganisms in the environment.
Errampalli
D
D
Agriculture and Agri-Food Canada, Charlottetown, PEI.
Leung
K
K
Cassidy
M B
MB
Kostrzynska
M
M
Blears
M
M
Lee
H
H
Trevors
J T
JT
D001709:Biotechnology; D004783:Environmental Microbiology; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism*
D005819:Genetic Markers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins