Acceleration of the refolding of Arc repressor by nucleic acids and other polyanions.
The refolding rate of the Arc repressor dimer can be accelerated 30-fold or more by negatively charged polymers including single-stranded and double-stranded DNA, RNA, and polyvinylsulfate but not by neutral or positively charged polymers. The salt-dependence of the polyanion-mediated process and mutant studies indicate that electrostatic interactions are important in the rate acceleration. Urea-dependence studies suggest that Arc is relatively unstructured in the transition state for polyanion-stimulated refolding. At low ionic strength, the observed kinetics of refolding are consistent with a model in which denatured Arc monomers bind rapidly and nonspecifically to the polyanion and complete folding in the bound state.
Rentzeperis
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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139-4703, USA.
Jonsson
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Sauer
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RT
D000818:Animals; D004247:DNA / Q000378:metabolism / Q000494:pharmacology*; D019281:Dimerization; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D009154:Mutation; D009994:Osmolar Concentration; D000071228:Polyelectrolytes; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology*; D011145:Polyvinyls / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D012313:RNA / Q000378:metabolism / Q000494:pharmacology*; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins; D014783:Viscosity
D004247:DNA; D000071228:Polyelectrolytes; D011108:Polymers; D011145:Polyvinyls; D012313:RNA; D012097:Repressor Proteins; D014508:Urea; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; C019050:polyvinyl sulfate