Effect of phenolic and chlorine disinfectants on hepatitis C virus binding and infectivity.
BACKGROUND
The purpose of this study was to evaluate the inhibitory activity of 2 polyphenolic disinfectants and a chlorine compound (NaDCC) on hepatitis C virus (HCV) binding and infectivity.


METHODS
VERO cells (a continuous cell line derived from cynomolgus kidney cells) suitable for analyzing HCV binding and replication, and the competitive reverse transcription (cRT-PCR) technique for HCV RNA molecules quantitative evaluation have been chosen as a methodologic approach for the antiviral activity testing.


RESULTS
At their recommended use dilutions, polyphenolic disinfectants inhibited HCV binding and replication. The chlorine compound was ineffective, probably a result of its low concentration in the presence of protein substances in VERO cell cultures.


CONCLUSIONS
Inhibition of HCV binding and replication by the tested polyphenolic associations confirm their value in instrument decontamination and environmental disinfection against this clinically important lipid virus.
Agolini
G
G
Department of Biomedical Sciences, University of Trieste, Italy.
Russo
A
A
Clementi
M
M
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D017606:Chlorine Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004202:Disinfectants / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016174:Hepacivirus / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D012367:RNA, Viral / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells / Q000187:drug effects / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D017606:Chlorine Compounds; D004202:Disinfectants; D010636:Phenols; D012367:RNA, Viral