title: Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines.
abstract: The Spodoptera exigua multinucleocapsid nucleopolyhedrovirus (SeMNPV) was inoculated to eight lepidopteran cell lines derived from Spodoptera exigua (Se301), Spodoptera frugiperda (SF21AEII), Spodoptera littoralis (CLS-79), Spodoptera litura (SpLi-221), Pseudaletia separata (LeSe-11), Trichoplusia ni (hi-5), Plutella xylostella (PXL/C) and Bombyx mori (BmN4). The productive infection of SeMNPV was observed only in Se301 cells. However, a dot-blot hybridization analysis revealed that SeMNPV DNA replicated in five non-permissive cell lines: SF21AEII, CLS-79, SpLi-221, hi-5 and BmN4. In addition, the virus-infected hi-5 and BmN4 cells displayed morphological changes. In contrast, CLS-79 cells inoculated with SeMNPV showed membrane blebbing at 20 hrs post inoculation (p.i.) and fragmentation of genomic DNA. All that indicated that the infected CLS-79 cells underwent apoptosis. These findings indicate that the SeMNPV replication was restricted at various points in dependence upon each cell line.
authors.affiliation: Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yanase
authors.forename: C
authors.initials: C
authors.lastname: Yasunaga
authors.forename: T
authors.initials: T
authors.lastname: Kawarabata
mesh_terms: D000818:Animals; D017209:Apoptosis; D012831:Bombyx; D002460:Cell Line / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D017924:Nucleopolyhedroviruses / Q000254:growth & development* / Q000302:isolation & purification; D013045:Species Specificity; D018411:Spodoptera; D013997:Time Factors; D014779:Virus Replication
chemical_list: D004279:DNA, Viral