B-cell pulmonary lymphoma: gene rearrangement analysis of bronchoalveolar lymphocytes by polymerase chain reaction.
OBJECTIVE
Non-Hodgkin's lymphomas (NHLs) are clonal proliferation of B or T lymphocytes. Assessment of clonality in lymphoid proliferations uses immunochemistry and, recently, molecular biology. The aim of our study is to assess the role of immunoglobulin gene rearrangement analysis on bronchoalveolar lymphocytes to aid in the diagnosis of B-cell pulmonary NHL.


METHODS
The study took place in a university hospital. There were seven consecutive patients with B-cell-type pulmonary lymphoma and nine control subjects. Gene rearrangement analysis using polymerase chain reaction (PCR) technique was performed on alveolar lymphocytes recovered by BAL.


RESULTS
Analysis of the immunoglobulin heavy chain gene rearrangement showed a predominant clonal alveolar lymphocyte population in six of seven patients while all control subjects showed germline pattern.


CONCLUSIONS
Gene rearrangement analysis by PCR of alveolar lymphocytes would appear to be sensitive in patients with B-cell pulmonary NHL (six of seven patients) and specific (zero of nine in the control group). This simple test should be added only in the analysis of cells recovered by BAL in patients with suspected primary and secondary B-cell pulmonary NHL.
Philippe
B
B
Department of Pulmonary Diseases, Foch Hospital, Suresnes, France. bphil@pasteur.fr
Delfau-Larue
M H
MH
Epardeau
B
B
Autran
B
B
Clauvel
J P
JP
Farcet
J P
JP
Couderc
L J
LJ
D000328:Adult; D000368:Aged; D016175:B-Lymphocyte Subsets; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005819:Genetic Markers; D006801:Humans; D016130:Immunophenotyping; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011446:Prospective Studies
D005819:Genetic Markers