title: Induction of cell shape changes through activation of the interleukin-3 common beta chain receptor by the RON receptor-type tyrosine kinase.
abstract: The RON receptor-type tyrosine kinase, a member of the hepatocyte growth factor receptor family, is a receptor for macrophage-stimulating protein (MSP). Recently, we observed that MSP induces morphological changes in interleukin (IL)-3-dependent Ba/F3 cells ectopically expressing RON. We show here that stimulation of those cells with either MSP or IL-3 increases tyrosine phosphorylation of proteins of 130, 110, 90, 62, and 58 kDa and induces similar morphological changes, accompanied by unique nuclear shape and redistribution of F-actin. A tyrosine kinase inhibitor, genistein, blocked both the increase in tyrosine phosphorylation and morphological changes. Upon stimulation with either MSP or IL-3, prominent tyrosine-phosphorylated pp90 was similarly co-immunoprecipitated with the common beta chain of IL-3 receptor (betac). Unlike IL-3, stimulation with MSP increased tyrosine phosphorylation of betac without activation of JAK2, resulting in morphological changes with modest cell growth. Confocal immunofluorescence analyses showed colocalization of RON, betac, and tyrosine-phosphorylated proteins. In vitro kinase assays revealed that autophosphorylated RON phosphorylated betac. These results suggest that the signaling pathway for morphological changes through betac and its associated protein pp90 is distinct from the pathway for cell growth in the IL-3 signal transduction system.
authors.affiliation: First Department of Internal Medicine, Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine Kumamoto 860-0811, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Mera
authors.forename: M
authors.initials: M
authors.lastname: Suga
authors.forename: M
authors.initials: M
authors.lastname: Ando
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: N
authors.initials: N
authors.lastname: Yamaguchi
mesh_terms: D000199:Actins / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects*; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D019833:Genistein / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D017228:Hepatocyte Growth Factor; D007377:Interleukin-3 / Q000494:pharmacology; D053614:Janus Kinase 2; D008858:Microscopy, Phase-Contrast; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D016185:Receptors, Interleukin-3 / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000199:Actins; D006133:Growth Substances; D007377:Interleukin-3; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D016185:Receptors, Interleukin-3; C082332:macrophage stimulating protein; D019000:Phosphotyrosine; D017228:Hepatocyte Growth Factor; D019833:Genistein; D011505:Protein-Tyrosine Kinases; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases; D053614:Janus Kinase 2