Protection of rabbits against lapinized rinderpest virus with purified envelope glycoproteins of peste-des-petits-ruminants and rinderpest viruses.
Haemagglutinin (HA) and fusion (F) proteins of peste-des-petits-ruminants virus (PPRV) and rinderpest virus (RPV) were purified by immunoaffinity chromatography. The purified proteins were characterized by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Rabbit hyperimmune sera were raised against the purified HA and F proteins and assayed by enzyme-linked immunosorbent assay (ELISA), haemagglutination-inhibition (HAI) and virus neutralization (VN) tests. The immunized animals were challenged with a virulent lapinized (rabbit-adapted) strain of RPV. Both HA and F proteins of PPRV protected rabbits against a lethal challenge with lapinized RPV. As expected, RPV HA and F proteins also conferred a similar protection against the homologous challenge. The postchallenge antibody responses were of a true anamnestic type.
Devireddy
L R
LR
Department of Veterinary Microbiology, Veterinary College, Bangalore, India.
Raghavan
R
R
Ramachandran
S
S
Subbarao
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D002522:Chlorocebus aethiops; D002846:Chromatography, Affinity; D005260:Female; D006389:Hemagglutinins, Viral / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012301:Rinderpest / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D012302:Rinderpest virus / Q000737:chemistry / Q000276:immunology*; D013997:Time Factors; D014709:Vero Cells / Q000821:virology; D014760:Viral Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; D014765:Viral Vaccines