[A study on diagnosis of oral squamous cell carcinoma (oral SCC) by glutathione S-transferase-pi (GST-pi)].
The purpose of this study was to estimate the usefulness of plasma GST-pi in diagnosis of oral SCC and to confirm the expression and localization of GST-pi in human oral SCC. Plasma GST-pi levels of 109 untreated oral SCC cases, 47 recurrent oral SCC cases and 36 healthy controls were measured by the sandwich enzyme-immunoassay (EIA) system. Human oral SCC and human oral SCC cell lines (HSC-3, HSC-4) were examined immunohistochemically and by the immunoelectron microscopic technique. The results were as follows: 1. The cut off level was 30.0 ng/ml (mean+2 S.D.). There was a statistically significant difference between the mean plasma GST-pi levels in oral SCC (both untreated and recurrent cases) and controls (p < 0.001). 2. The positive rate of GST-pi was 63.3% in untreated cases and 68.1% in recurrent cases. 3. There was no significant correlation between plasma GST-pi levels and each clinical factor. 4. The positive rates of plasma GST-pi were compared with those of serum SCC antigen in oral SCC. Plasma GST-pi was 62.9%, while serum SCC antigen 22.2%. 5. From immunohistochemical observation, GST-pi positive staining was evident in the cytoplasm and nucleus of oral SCC. 6. By immunoelectron microscopy, GST-pi was confirmed to be in the mitochondria and the inter-chromatinic regions of the nucleus.
Kobayashi
Y
Y
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis*
D014408:Biomarkers, Tumor; D005982:Glutathione Transferase