Expansion and immunological study of human tumor infiltrating gamma/delta T lymphocytes in vitro.
Goffa/delta T cells have stimulated a lot of interest because of their unique features in antigen recognition and cytotoxicities to many autologous and/or allogeneic tumor cells. We have developed a novel method to selectively expand larger amounts of human tumor-infiltrating gamma/delta T lymphocytes (gamma/delta TILs) ex vivo by immobilized pan- anti-TCRgamma/delta monoclonal antibody in the presence of exogenous IL-2. The expanded gamma/delta TILs mainly expressed CD45RO and HLA-DR molecules and did not express CD4. CD8+ gamma/delta TILs accounted for 19% of gamma/delta TILs. The expression of CD25 molecule on expanded gamma/delta T cells was inducible and downregulated following a time course. The Vdelta1 and Vdelta2 subsets amount to 37 and 58%, respectively. The expanded gamma/delta TILs show an IL-2-dependent proliferation, MHC class I-unrestricted and TCRgamma/delta-related cytotoxicities to two MHC class I+ and two MHC class I+ allogeneic tumor cell lines in vitro.
Yu
S
S
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, People's Republic of China.
He
W
W
Chen
J
J
Zhang
F
F
Ba
D
D
D000230:Adenocarcinoma / Q000276:immunology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D016692:Receptors, Antigen, T-Cell, gamma-delta