[Extracellular components of broncho-alveolar lavage fluid (BALF) as markers of interstitial pulmonary disease activity. II. Phospholipid concentration].
Phospholipids found in the alveolar space come from the surfactant which is produced by type II pneumocytes and whose surplus is eliminated from lungs by macrophages. In 188 patients with interstitial pulmonary diseases bronchoalveolar lavage was performed. Phospholipids from the supernatant of BALF were extracted using of Folch method and their concentration was measured colorimetrically assaying inorganic phosphorus after prior mineralization by Fiske-Subbarov method. The persons were divided into 7 groups: active sarcoidosis, inactive sarcoidosis, avian fancier's lung in the period of contact with the antigen, and after stopping the contact with the antigen, advanced idiopathic pulmonary fibrosis, moderate idiopathic pulmonary fibrosis, and a control group of healthy persons. Apart from that, the patients were divided into untreated and treated with corticosteroids. In every group of patients we have noticed increased concentration and total amount of phospholipids in BALF. Particularly distinct increase of the total number of phospholipids in BALF in pulmonary fibrosis was observed. However, this parameter does not permit to estimate disease activity in interstitial lung disease. Corticotherapy increases phospholipids concentration in BALF in patients with idiopathic pulmonary fibrosis and avian fancier's lung but decreases in sarcoidosis patients.
Zielonka
T M
TM
Kliniki Pneumonologii, Akademii Medycznej w Warszawie.
Wałajtys-Rode
E
E
Jasińska
R
R
Chazan
R
R
Droszcz
W
W
D015415:Biomarkers / Q000032:analysis; D001716:Bird Fancier's Lung / Q000175:diagnosis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000175:diagnosis*; D010743:Phospholipids / Q000032:analysis*; D011658:Pulmonary Fibrosis / Q000175:diagnosis; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis
D015415:Biomarkers; D010743:Phospholipids