title: [Importance of liver puncture biopsy and endoscopic retrograde cholangiography in patients with chronic anicteric unexplained cholestasis. A retrospective study in 79 patients].
abstract: OBJECTIVE
  To determine the diagnostic value of systematic liver needle biopsy and endoscopic retrograde cholangiography in patients with unexplained chronic anicteric cholestasis.
  
  
  METHODS
  Seventy nine patients presented with anicteric cholestasis for over 6 months as defined by: a concomitant increase in at least 2 of 3 cholestatic enzymes (GGT, alkaline phosphatase, 5'nucleotidase); a low cytolytic ratio (ALT/AP (xN/xN) < or = 5); and negative test results (normal ultrasound scan; no antimitochondrial antibodies, viral, drug-induced, or toxic hepatitis, or known ulcerative cholitis). Based on liver biopsy and endoscopic retrograde cholangiography, 5 groups were determined; group A: normal liver biopsy and endoscopic retrograde cholangiography; group B: primary sclerosing cholangitis with histological biliary lesions; group C: primary sclerosing cholangitis with normal histology; group D: histologic biliary lesions alone; group E: other (aspecific histologic lesions, isolated anomalies of intrahepatic bile ducts on endoscopic retrograde cholangiography).
  
  
  RESULTS
  Diagnosis of cholestasis was fortuitous in 43% of cases. Group A: 5 patients had normal liver biopsy and endoscopic retrograde cholangiography; group B (10 patients): 5 with destructive cholangitis, 5 with degenerative cholangitis, associated with portal fibrosis in 90%; group C: none of the patients had primary sclerosing cholangitis with normal histology; group D: 39 patients {idiopathic ductopenia (1), Caroli's disease (1), benign recurrent cholestasis (1), regenerative nodular hyperplasia (4), destructive cholangitis without ductopenia (7), degenerative cholangitis (15), ductular proliferation (10)}; group E: 24 patients with aspecific histologic lesions, and one patient with isolated anomalies of the intrahepatic bile ducts on endoscopic retrograde cholangiography.
  
  
  CONCLUSIONS
  In the present population: a) 13% presented with intense cholangitis and primary sclerosing cholangitis on endoscopic retrograde cholangiography; b) 49% presented with various histologic biliary lesions without primary sclerosing cholangitis. We conclude that in chronic anicteric cholestasis of unexplained origin, first choice work-up should include liver biopsy, and endoscopic retrograde cholangiography should only be performed when intense histologic cholangitis is observed.
authors.affiliation: Clinique des Maladies du Foie et INSERM U49, Centre Hospitalier Universitaire Pontchaillou, Rennes.
authors.forename: T
authors.initials: T
authors.lastname: Sapey
authors.forename: B
authors.initials: B
authors.lastname: Turlin
authors.forename: V
authors.initials: V
authors.lastname: Canva-Delcambre
authors.forename: M H
authors.initials: MH
authors.lastname: Mendler
authors.forename: Y
authors.initials: Y
authors.lastname: Deugnier
authors.forename: P
authors.initials: P
authors.lastname: Brissot
authors.forename: D
authors.initials: D
authors.lastname: Guyader
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001652:Bile Ducts / Q000473:pathology*; D001706:Biopsy; D002758:Cholangiography / Q000379:methods*; D015209:Cholangitis, Sclerosing / Q000175:diagnosis*; D002780:Cholestasis, Intrahepatic / Q000175:diagnosis*; D002908:Chronic Disease; D004724:Endoscopy; D005260:Female; D006801:Humans; D008099:Liver / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies