The chronic cell death with DNA fragmentation after post-ischaemic hypothermia in the gerbil hippocampus.
The long-term effects of post-ischaemic hypothermia are controversial. The purpose of this study was to examine the long-term effects of post-ischaemic hypothermia on neuronal survival in gerbils in terms of morphology and function. Hypothermia was induced at 32 degrees C for 4 h immediately after ischaemia. Examination was performed at 1 week and at 1 month after ischaemia. Post-ischaemic hypothermia prevented CA1 neuronal damage 1 week after ischaemia. At 1 month after ischaemic insult, however, the degree of the protective effect of post-ischaemic hypothermia was reduced in the lateral and medial CA1 areas. DNA fragmentation was also observed at 1 month. The errors in the 8-arm radial maze trial were increased at 1 month. These data may indicate that cells in the CA1 area are very vulnerable to ischaemia and die after post-ischaemic hypothermia, and that their death is associated with apoptosis.
Nakamura
T
T
Department of Neurological Surgery, Kagawa Medical University, Japan.
Miyamoto
O
O
Yamagami
S
S
Toyoshima
T
T
Negi
T
T
Itano
T
T
Nagao
S
S
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000150:complications / Q000473:pathology / Q000628:therapy*; D002470:Cell Survival; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D005849:Gerbillinae; D006624:Hippocampus / Q000473:pathology; D007036:Hypothermia, Induced; D008297:Male; D008569:Memory Disorders / Q000209:etiology / Q000517:prevention & control; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors