A new biocatalyst for production of optically pure aryl epoxides by styrene monooxygenase from Pseudomonas fluorescens ST.
We developed a biocatalyst by cloning the styrene monooxygenase genes (styA and styB) from Pseudomonas fluorescens ST responsible for the oxidation of styrene to its corresponding epoxide. Recombinant Escherichia coli was able to oxidize different aryl vinyl and aryl ethenyl compounds to their corresponding optically pure epoxides. The results of bioconversions indicate the broad substrate preference of styrene monooxygenase and its potential for the production of several fine chemicals.
Di Gennaro
P
P
Department of Genetics and Biology of Microorganisms, University of Milan, 20133 Milan, Italy.
Colmegna
A
A
Galli
E
E
Sello
G
G
Pelizzoni
F
F
Bestetti
G
G
D002384:Catalysis; D004852:Epoxy Compounds / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D004852:Epoxy Compounds; D011994:Recombinant Proteins; D010105:Oxygenases; C026721:styrene monooxygenase