Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein.
This study compares the immune responses produced by immunising mice and rabbits with two preparations of the recombinant 15/60 kDa protein of Cryptosporidium parvum. Genomic C. parvum DNA was amplified and the recombinant protein was synthesized as a fusion protein with glutathione-S-transferase in Escherichia coli and in the eukaryotic system of baculovirus/insect cells. Both recombinant proteins induced similar levels of serum antibodies against the fusion recombinant protein, but the eukaryotic recombinant protein triggered a stronger humoral response to C. parvum. Similarly, increased lymphoproliferation occurred only after stimulation of spleen cells from mice immunised with the eukaryotic recombinant protein. This suggests that the eukaryotic protein is a better candidate for immunological studies on cryptosporidiosis.
Iochmann
S
S
Equipe INRA d>>Immunologie Parasitaire EA 2637, Laboratoire de Virologie, UFR des Sciences Pharmaceutiques, 31 avenue Monge, Tours, 37200, France.
Sagodira
S
S
Mévélec
M N
MN
Répérant
J M
JM
Naciri
M
M
Coursaget
P
P
Bout
D
D
D000818:Animals; D000917:Antibody Formation; D018797:Cell Cycle Proteins; D002455:Cell Division; D002460:Cell Line; D016785:Cryptosporidium parvum / Q000235:genetics / Q000276:immunology*; D029721:Drosophila Proteins; D005057:Eukaryotic Cells; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000276:immunology*; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology*; D011387:Prokaryotic Cells; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014614:Vaccines, Synthetic / Q000276:immunology*
C094890:CP15 protein, Cryptosporidium parvum; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C098388:Map60 protein, Drosophila; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase