Microcapsules prepared from alginate and a photosensitive poly(L-lysine).
A photosensitive polymer, alpha-phenylcinnamylideneacetylated poly(L-lysine), was synthesized and characterized. This photosensitive poly(L-lysine) had 10% of its lysine residues reacted with alpha-phenylcinnamylidene acetyl group and displayed an absorption maximum at 329 nm. The photosensitive poly(L-lysine) was used for the preparation of microcapsules. The capsules formed from this photosensitive poly(L-lysine) and alginate were strengthened significantly by light irradiation. The photo cross-linked capsular membrane was permeable to proteins with mass transfer rate in the descending order: cytochrome C, myoglobin. and serum albumin. GH3 (a rat pituitary tumor cell line) cells were encapsulated and cultured with this microencapsulation system. The cells proliferated to a density of about 4 x 10(5) cells ml(-1) in the capsules after 6 days cultivation.
Chang
S J
SJ
Institute of Biomedical Engineering, National Yang Ming University Shih Pai, Taipei, Taiwan, ROC.
Lee
C H
CH
Wang
Y J
YJ
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules / Q000138:chemical synthesis* / Q000737:chemistry*; D002452:Cell Count; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D009682:Magnetic Resonance Spectroscopy; D011107:Polylysine / Q000737:chemistry*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D011107:Polylysine; D020723:Glucuronic Acid