title: The impact of intraoperative culture on treatment and outcome in children with perforated appendicitis.
abstract: BACKGROUND
  Most protocols for the operative treatment of perforated appendicitis use a routine culture. Although isolated studies suggest that routine culture may not be necessary, these recommendations generally are not based on objective outcome data.
  
  
  METHODS
  The authors reviewed the records of 308 children who underwent operative treatment for perforated appendicitis between 1988 and 1998 to determine if information gained from routine culture changes the management or improves outcome. Inclusion criteria included either gross or microscopic evidence of appendiceal perforation.
  
  
  RESULTS
  Mean patient age was 7.5 years, 51% were boys, and there was no mortality. The majority of children (96%) underwent culture that was positive for either aerobes (21%), anaerobes (19%), or both (57%). Antibiotics were changed in only 16% of the patients in response to culture results. The use of empiric antibiotics, as compared with modified antibiotics, was associated with a lower incidence of infectious complication, shorter fever duration, and decreased length of hospitalization. We also investigated the relationship between culture isolates and antibiotic regimens with regard to outcome. The utilization of antibiotics suitable for the respective culture isolate or organism sensitivity was associated with an increased incidence of infectious complication and longer duration of both fever and length of hospitalization. Finally, the initial culture correlated poorly with subsequent intraabdominal culture (positive predictive value, 11%).
  
  
  CONCLUSIONS
  These outcome data strongly suggest that the practice of obtaining routine cultures can be abandoned, and empiric broad spectrum antibiotic coverage directed at likely organisms is completely adequate for treatment of perforated appendicitis in children.
authors.affiliation: Department of Surgery, St Louis University Health Sciences Center and Cardinal Glennon Children's Hospital, MO 63104, USA.
authors.forename: E R
authors.initials: ER
authors.lastname: Kokoska
authors.forename: M L
authors.initials: ML
authors.lastname: Silen
authors.forename: T F
authors.initials: TF
authors.lastname: Tracy
authors.forename: P A
authors.initials: PA
authors.lastname: Dillon
authors.forename: D J
authors.initials: DJ
authors.lastname: Kennedy
authors.forename: T V
authors.initials: TV
authors.lastname: Cradock
authors.forename: T R
authors.initials: TR
authors.lastname: Weber
mesh_terms: D000293:Adolescent; D001064:Appendicitis / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery*; D001202:Ascitic Fluid / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007416:Intestinal Perforation / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery*; D007432:Intraoperative Period; D008297:Male; D013048:Specimen Handling; D016896:Treatment Outcome