Gene transfer mediated by YKS-220 cationic particles: convenient and efficient gene delivery reagent.
A monocationic lipid, YKS-220, with a symmetrical and biodegradable structure can be used as an effective gene transfer vector in a cationic particle form (not a cationic liposome form), and is obtained by diluting an ethanol solution of YKS-220 and DOPE (1:5, molar ratio) with an aqueous medium. This preparation method is more convenient than that for cationic liposomes. YKS-220 cationic particles showed a heterogeneous large mean diameter of 4.4 microm. An obvious size change was not observed when plasmid DNA was added. The transfection activity of YKS-220 cationic particles was comparable to those of YKS-220 liposomes and DOSPA liposomes (LipofectAMINE), and even higher than that of DOGS (TRNSFECTAM). Interestingly, the YKS-220 cationic particle/DNA complexes were resistant to the neutralizing effect of serum. All of these findings indicate that YKS-220 cationic particles are a convenient and efficient gene delivery reagent.
Yu
W
W
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan.
Shimoyama
A
A
Uneda
T
T
Obika
S
S
Miyashita
K
K
Doi
T
T
Imanishi
T
T
D000818:Animals; D002411:Cation Exchange Resins; D002412:Cations; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005229:Fatty Acids, Monounsaturated; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005998:Glycine / Q000031:analogs & derivatives; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D008156:Luciferases / Q000235:genetics; D010316:Particle Size; D010714:Phosphatidylethanolamines; D010957:Plasmids / Q000235:genetics; D000644:Quaternary Ammonium Compounds; D013096:Spermine / Q000031:analogs & derivatives; D014162:Transfection
D002411:Cation Exchange Resins; D002412:Cations; D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C118640:YKS 220; C061134:dioctadecylamidoglycylspermine; D013096:Spermine; C020888:1,2-dielaidoylphosphatidylethanolamine; D008156:Luciferases; D005998:Glycine