title: Possible causes of diabetes mellitus in cattle infected with bovine viral diarrhoea virus.
abstract: In cattle, we encountered insulin-dependent diabetes mellitus (IDDM) associated with bovine viral diarrhoea virus (BVDV) infection. To estimate the correlation between IDDM and BVDV infection, the distribution of BVDV in the pancreas and islet-cell antibody (ICA) were investigated. The distribution of BVDV in the pancreas was examined by in situ hybridization using two oligonucleotide probes that recognized the gp25- and p14-coding regions of the BVDV gene. ICA was examined by indirect fluorescence antibody assay using the sera from affected cattle and pancreata from normal cattle. In the pancreata of all BVDV-infected cattle, including IDDM-complicated cattle, oligonucleotide probe hybridized portions were recognized. In short, BVDV genes were detected not only in IDDM-complicated cattle but also in uncomplicated cattle. Moreover, there was no hybridized portion in the islet cells. In BVDV-infected and IDDM-complicated cattle, ICA was frequently detected. On the other hand, ICA was not detected in BVDV-infected and IDDM uncomplicated cattle. These results suggest that IDDM associated with BVDV infection is not a direct effect of BVDV on islet cells. Therefore, as BVDV did not induce IDDM in any cases, it appears that BVDV does not induce IDDM directly, but rather may be an autoimmune disease induced by autoantibodies against islet cells.
authors.affiliation: Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tajima
authors.forename: M
authors.initials: M
authors.lastname: Yuasa
authors.forename: M
authors.initials: M
authors.lastname: Kawanabe
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
authors.forename: O
authors.initials: O
authors.lastname: Yamato
authors.forename: Y
authors.initials: Y
authors.lastname: Maede
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000150:complications* / Q000473:pathology; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology / Q000821:virology*; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D005260:Female; D017403:In Situ Hybridization; D007515:Islets of Langerhans / Q000276:immunology; D010179:Pancreas / Q000473:pathology / Q000821:virology*
chemical_list: D001323:Autoantibodies; C024211:islet cell antibody