title: The molybdenum cofactor biosynthesis protein MobA from Rhodobacter capsulatus is required for the activity of molybdenum enzymes containing MGD, but not for xanthine dehydrogenase harboring the MPT cofactor.
abstract: The requirement of MobA for molybdoenzymes with different molybdenum cofactors was analyzed in Rhodobacter capsulatus. MobA is essential for DMSO reductase and nitrate reductase activity, both enzymes containing the molybdopterin guanine dinucleotide cofactor (MGD), but not for active xanthine dehydrogenase, harboring the molybdopterin cofactor. In contrast to the mob locus of Escherichia coli and R. sphaeroides, the mobB gene is not located downstream of mobA in R. capsulatus. The mobA gene is expressed constitutively at low levels and no increase in mobA expression could be observed even under conditions of high MGD demand.
authors.affiliation: Ruhr-Universität Bochum, Fakultät für Biologie, Bochum, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Leimkühler
authors.forename: W
authors.initials: W
authors.lastname: Klipp
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006150:Guanine Nucleotides / Q000378:metabolism*; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D016254:Mutagenesis, Insertional; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D011621:Pteridines / Q000378:metabolism*; D011622:Pterins / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000201:enzymology* / Q000235:genetics; D013466:Sulfurtransferases / Q000378:metabolism; D014968:Xanthine Dehydrogenase / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006150:Guanine Nucleotides; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D050901:Nitrate Reductase; D009565:Nitrate Reductases; D010088:Oxidoreductases; D011621:Pteridines; D011622:Pterins; D013466:Sulfurtransferases; D014968:Xanthine Dehydrogenase; C095754:mobA protein, E coli; C063761:molybdopterin guanine dinucleotide; C022261:molybdopterin; C045664:dimethyl sulfoxide reductase; C085585:molybdopterin synthase