A novel plant nuclear gene encoding chloroplast ribosomal protein S9 has a transit peptide related to that of rice chloroplast ribosomal protein L12.
We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluorescence was detectable only in chloroplasts, indicating that this nuclear gene encodes chloroplast ribosomal protein S9. The N-terminal region of rice ribosomal protein S9 was found to have a high sequence similarity to the transit peptide region of the rice chloroplast ribosomal protein L12, suggesting that these transit peptides have a common lineage.
Arimura
S
S
Laboratory of Radiation Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Takusagawa
S
S
Hatano
S
S
Nakazono
M
M
Hirai
A
A
Tsutsumi
N
N
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus; D002736:Chloroplasts / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant; D020224:Expressed Sequence Tags; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012275:Oryza; D010455:Peptides / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018744:DNA, Plant; D009687:Nuclear Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12