title: Epidemiological analysis of Salmonella enteritidis from human outbreaks by pulsed-field gel electrophoresis.
abstract: To determine the extent of genetic diversity among isolates of Salmonella enteritidis obtained from outbreaks in Fukuoka Prefecture, Japan, from 1989 to 1994, we analyzed a total of 55 isolates of S. enteritidis obtained from 13 distinct outbreaks with pulsed-field gel electrophoresis. These isolates showed three different patterns in pulsed-field profile with Bln I digestion. The groups A, B and C consisted of three outbreaks isolates (Dice coefficient, F = 1), of seven outbreaks (F = 0.56-0.94) and of three outbreaks (F = 0.65-0.78), respectively. This result suggests that a few limited clonal lines of S. enteritidis were successively causing outbreaks in this area from 1989 to 1994.
authors.affiliation: Pathology and Bacteriology Division, Fukuoka Institute of Health and Environmental Sciences, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Horikawa
authors.forename: K
authors.initials: K
authors.lastname: Otsuki
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004196:Disease Outbreaks / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D014644:Genetic Variation; D006801:Humans; D012481:Salmonella Infections, Animal / Q000453:epidemiology*; D012477:Salmonella enteritidis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C064053:CCTAGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific