title: Molecular analysis of Pseudomonas aeruginosa: epidemiological investigation of mastitis outbreaks in Irish dairy herds.
abstract: Pseudomonas aeruginosa is a pathogen in both humans and animals. This bacterium, most often associated with respiratory infections in cystic fibrosis patients, was found to be the causative agent in bovine mastitis outbreaks among 11 Irish dairy herds. Epidemiological findings suggested that the infection was spread to all herds by teat wipes that had been contaminated with this organism. Two molecular-typing strategies were used in an attempt to determine the genomic relationship(s), if any, of the P. aeruginosa strains isolated from the various herds and to verify whether the same strain was responsible for each outbreak. Thirty-six isolates from the mastitis outbreaks were tested and compared to fourteen clinical isolates from Cork University Hospital. With one exception, all outbreak-linked strains produced identical patterns when ribotyped with ClaI and PvuII enzymes. Eight of the clinical isolates gave the same ClaI ribotype pattern as the mastitis-causing strains. However, PvuII proved more discriminatory, with only the outbreak isolates producing identical patterns. Similar results were obtained with RW3A-primed DNA amplification fingerprinting, with all outbreak isolates except one displaying the same fingerprint array. The clinical strains produced several fingerprint patterns, all of which were different from those of the mastitis-causing isolates. Fine-resolution DNA fingerprinting with a fluorescence-labelled RW3A primer also identified a number of low-molecular-weight polymorphisms that would have remained undetected by conventional methods. These data support the view that the same P. aeruginosa strain was responsible for the mastitis outbreaks in all 11 herds.
authors.affiliation: Molecular Diagnostics Unit, Cork Institute of Technology, Bishopstown, Cork, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Daly
authors.forename: E
authors.initials: E
authors.lastname: Power
authors.forename: J
authors.initials: J
authors.lastname: Björkroth
authors.forename: P
authors.initials: P
authors.lastname: Sheehan
authors.forename: A
authors.initials: A
authors.lastname: O'Connell
authors.forename: M
authors.initials: M
authors.lastname: Colgan
authors.forename: H
authors.initials: H
authors.lastname: Korkeala
authors.forename: S
authors.initials: S
authors.lastname: Fanning
mesh_terms: D000818:Animals; D002417:Cattle; D003550:Cystic Fibrosis / Q000382:microbiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D003612:Dairying; D004196:Disease Outbreaks; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D008414:Mastitis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D017720:Molecular Epidemiology; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal