title: An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies.
abstract: A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi-solid media is described. The accumulation of metaphases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly-L-lysine-treated slides and fixed stepwise prior to staining. The technique produced high yields of well-spread metaphases facilitating clonal cytogenetic analysis.
authors.affiliation: Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire.
authors.forename: M A
authors.initials: MA
authors.lastname: Kadhim
authors.forename: D A
authors.initials: DA
authors.lastname: Pocock
authors.forename: S A
authors.initials: SA
authors.lastname: Lorimore
authors.forename: E G
authors.initials: EG
authors.lastname: Wright
mesh_terms: D000818:Animals; D002875:Chromosomes; D003582:Cytogenetics / Q000379:methods*; D006412:Hematopoietic Stem Cells; D051379:Mice