[The use of minipigs to produce diagnostic sera for the serological identification of bacteria in the genus Morganella].
Mini-pigs of the Svetlogorsk breed were immunized with vaccine prepared from of M. morganii culture; the culture had been grown in a liquid nutrient medium, obtained from casein hydrolysate, in flasks placed in a thermostatically controlled shaker to the phases of exponential growth and cell growth deceleration. O-agglutinating sera thus obtained were specific. The highest antibody level was detected in the serum obtained as the result of the immunization of guinea pigs with the vaccine prepared from the culture on the phase of exponential growth. This serum retained its properties after storage for 9 months. Heterologous antibodies occurred in the sera under study not more frequently than in rabbit sera and practically in the same titers.
Gurdina
V V
VV
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Mel'nikova
V A
VA
Zaĭtsev
T A
TA
Osipov
V V
VV
Romanenko
E E
EE
Adlova
G P
GP
Baturo
A P
AP
D000373:Agglutinins / Q000302:isolation & purification; D000818:Animals; D000918:Antibody Specificity; D001428:Bacterial Vaccines / Q000276:immunology; D004755:Enterobacteriaceae / Q000145:classification* / Q000276:immunology; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization / Q000379:methods; D008297:Male; D012703:Serotyping / Q000379:methods*; D013552:Swine; D013556:Swine, Miniature / Q000276:immunology*
D000373:Agglutinins; D001428:Bacterial Vaccines; D007106:Immune Sera