Ternary conjugates of guanosine monophosphate as initiator nucleotides for the enzymatic synthesis of 5'-modified RNAs.
We give a detailed account on the enzymatic synthesis of RNA conjugates by T7 RNA polymerase using modified initiator nucleotides during transcription. Following two different routes, ternary conjugates of guanosine-5'-monophosphate, poly(ethylene glycol), and anthracene were synthesized via phosphoramidite intermediates and characterized by a variety of spectroscopic techniques. Up to a degree of polymerization nPEG of about 17, these conjugates were efficiently incorporated into RNA by T7 RNA polymerase at the 5'-termini, thereby giving access to RNA conjugates required for biochemical studies as well as for the exploration of the catalytic potential of ribonucleic acids. The resulting conjugates are intact and functional.
Seelig
B
B
Institut für Biochemie, Freie Universität Berlin, Thielallee 63, D-14195 Berlin.
Jäschke
A
A
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D006157:Guanosine Monophosphate / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins
D004798:Enzymes; D014764:Viral Proteins; D011092:Polyethylene Glycols; D012313:RNA; D006157:Guanosine Monophosphate; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases