title: Overlapping between fluorescence modifications and activation of prostate transglutaminase induced by sodium dodecyl sulfate.
abstract: The transglutaminase from rat coagulating gland secretion has been proposed as a new member of the transglutaminase family. Its basal activity is about 11-fold lower than those of other transglutaminases (e.g., the cytosolic tissue transglutaminase), but reaches levels comparable to those of other transglutaminases on addition of specific surfactant agents. There is no study devoted to understanding the molecular basis of this apparently anomalous activation, which is maximal at approximately 1.5 mM sodium dodecyl sulfate. We provide evidence that in the presence of this detergent modifications of the intrinsic fluorescence as well as energy transfer of the protein fluorescence to a micellar probe parallel the activation of the enzyme. As the sodium dodecyl sulfate concentration inducing maximal activation equals the critical micellar concentration, the biological activity of this transglutaminase appears to be modulated by the binding of micellar aggregates. In fact, the enzyme is modified by posttranslational modifications consisting of some lipid tails. At least two of these tails could act as aggregation nuclei of the enzyme with detergents. This behavior is different from that typical of molecular forms purified from other sources.
authors.affiliation: Dipartimento di Biochimica e Biofisica "F. Cedrangolo,", Seconda Università di Napoli, via Costantinopoli 16, Naples, 80138, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Esposito
authors.forename: I
authors.initials: I
authors.lastname: Caputo
authors.forename: P
authors.initials: P
authors.lastname: Colicchio
authors.forename: R
authors.initials: R
authors.lastname: Porta
authors.forename: R
authors.initials: R
authors.lastname: Ragone
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D005453:Fluorescence; D008297:Male; D008956:Models, Chemical; D011467:Prostate / Q000201:enzymology*; D011499:Protein Processing, Post-Translational; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D011503:Transglutaminases / Q000187:drug effects*
chemical_list: D012967:Sodium Dodecyl Sulfate; D011503:Transglutaminases