title: Phospholipase D as an effector for ADP-ribosylation factor in the regulation of vesicular traffic.
abstract: A mammalian phospholipase D (PLD) activity that is stimulated by ADP-ribosylation factor (ARF) has been identified in Golgi-enriched membrane fractions. This activity is due to the PLD1 isoform and evidence from several laboratories indicates that PLD1 is important for the polymerization of vesicle coat proteins on membranes. When expressed in Chinese hamster ovary cells, PLD1 localized to dispersed small vesicles that overlapped with the location of the ERGIC53 protein, a marker for the endoplasmic reticulum (ER)-Golgi intermediate compartment. Cells having increased PLD1 expression had accelerated anterograde and retrograde transport between the ER and Golgi. Membranes from cells having elevated PLD1 activity bound more COPI, ARF, and ARF-GTPase activating protein. These membranes also produced more COPI vesicles than did membranes from control cells. It is likely that PLD1 participates in both positive and negative feedback regulation of the formation of COPI vesicles and is important for controlling the rate of this process.
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 75229, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Roth
authors.forename: K
authors.initials: K
authors.lastname: Bi
authors.forename: N T
authors.initials: NT
authors.lastname: Ktistakis
authors.forename: S
authors.initials: S
authors.lastname: Yu
mesh_terms: D020727:ADP-Ribosylation Factors; D000818:Animals; D016466:CHO Cells; D002352:Carrier Proteins / Q000378:metabolism; D020756:Coatomer Protein; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008322:Mammals; D008565:Membrane Proteins / Q000378:metabolism; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D020756:Coatomer Protein; D008565:Membrane Proteins; D011994:Recombinant Proteins; D010739:Phospholipase D; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors