title: Effect of intracellular Ca2+ chelation with the acetoxymethyl ester-derived form of bis(o-aminophenoxy)ethane-N,N,N,N',N'-tetraacetic acid on meiotic division and chromosomal segregation in mouse oocytes.
abstract: OBJECTIVE
  Our purpose was to ascertain the effect of intracellular Ca2+ chelation on the chromosomal distribution and segregation of mouse oocytes during maturation in vitro.
  
  
  METHODS
  Germinal vesicle oocytes were loaded with the acetoxymethyl ester-derived form of bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA-AM). Chromosomal distribution and segregation of control and BAPTA-AM-treated metaphase II (MII) oocytes were evaluated at 16 hr, and intracellular ATP content at 0, 1, and 16 hr after BAPTA-AM loading.
  
  
  RESULTS
  BAPTA-AM treatment decreased (P < or = 0.05) the potential for in vitro maturation, increased (P < or = 0.0001) the percentage of oocytes displaying an abnormal distribution of metaphase II chromosomes in the meiosis II spindle and aneuploidy, and decreased (P < or = 0.005) the ATP content at 0, 1, and 16 hr of culture compared to the control groups.
  
  
  CONCLUSIONS
  These findings raise some concern about any other condition/drug that may directly or indirectly decrease the intracellular Ca2+ concentration in human oocytes.
authors.affiliation: Department of Paediatrics, Obstetrics and Gynaecology, Faculty of Medicine, University of Valencia, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Vendrell
authors.forename: J
authors.initials: J
authors.lastname: Ten
authors.forename: M N
authors.initials: MN
authors.lastname: De Oliveira
authors.forename: A
authors.initials: A
authors.lastname: Cano
authors.forename: J J
authors.initials: JJ
authors.lastname: Tarin
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000782:Aneuploidy; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002875:Chromosomes / Q000187:drug effects*; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*
chemical_list: C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D002118:Calcium