Detection of high levels of canine herpes virus-1 neutralising antibody in kennel dogs using a novel serum neutralisation test.
It is widely held that only cells of canine origin support canine herpesvirus-1 (CHV-1) replication and, that cytopathic effect (CPE) develops relatively slowly. Here we show that mink fetal lung cells (NBL-7 cell line) are permissive for CHV-1 and can be used to produce a sensitive test for neutralising antibody by plaque reduction in the presence of complement. The test was applied to the investigation of CHV-1 virus neutralising antibody levels in three kennel populations. The results showed that 26 out of 28 dogs were neutralising antibody positive (titre >/=2), and, 11 out of 28 had titres of >/=1024. The serum samples were analysed by enzyme linked immunoassay (ELISA); 27 out of 28 were graded as ELISA IgG positive (titre >/=500) and 26 of 28 were graded as ELISA IgM positive (titre >/=50).
Reading
M J
MJ
Centre for Veterinary Science, Cambridge University, Department of Clinical Veterinary Medicine, Cambridge, CB3 0ES, UK.
Field
H J
HJ
D016475:3T3 Cells; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D002184:Herpesvirus 1, Canid / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008907:Mink; D009500:Neutralization Tests / Q000662:veterinary; D011817:Rabbits
D000914:Antibodies, Viral