Two-dimensional map of human brain proteins.
Samples of human brain from the parietal cortex lobe were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradient strips covering the various pH regions. The protein spots were visualized with colloidal Coomassie blue stain and identified by matrix-assisted laser desorption/ionization mass spectrometry. Approximately 400 spots were identified, corresponding to 180 different brain proteins. The list of identified proteins includes a large number of structural proteins and of enzymes or enzyme subunits with various catalytic activities. The majority of proteins are localized in the cytoplasma and in mitochondria. The two-dimensional map may be useful as a reference database to study changes in the protein level caused by various disorders, such as Alzheimer's disease, major depression and schizophrenia.
Langen
H
H
F. Hoffmann-La Roche, Pharmaceutical Research-Gene Technology, Basel, Switzerland.
Berndt
P
P
Röder
D
D
Cairns
N
N
Lubec
G
G
Fountoulakis
M
M
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D051379:Mice; D008875:Middle Aged; D010296:Parietal Lobe / Q000737:chemistry; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011506:Proteins