Inhibitory effect of a self-derived peptide on glucosyltransferase of Streptococcus mutans. Possible novel anticaries measures.
Glucosyltransferase (GTF) plays an important role in the development of dental caries. We examined the possible presence of self-inhibitory segments within the enzyme molecule for the purpose of developing anticaries measures through GTF inhibition. Twenty-two synthetic peptides derived from various regions presumably responsible for insoluble-glucan synthesis were studied with respect to their effects on catalytic activity. One of them, which is identical in amino acid sequence to residues 1176-1194, significantly and specifically inhibited both sucrose hydrolysis and glucosyl transfer to glucan by GTF-I. Double-reciprocal analysis revealed that the inhibition is noncompetitive. Scramble peptides, composed of the identical amino acids in randomized sequence, had no effect on GTF-I activity. Furthermore, the peptide is tightly bound to the enzyme once complexed, even in the presence of sodium dodecyl sulfate (SDS). Kinetic analysis using an optical evanescent resonant mirror cuvette system demonstrated that the enzyme-peptide interaction was biphasic. These results indicate that the peptide directly interacts with the enzyme with high affinity and inhibits its activity in a sequence-specific manner. This peptide itself could possibly be an effective agent for prevention of dental caries, although its effectiveness may be improved by further modification.
Eto
A
A
Department of Oral Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Saido
T C
TC
Fukushima
K
K
Tomioka
S
S
Imai
S
S
Nisizawa
T
T
Hanada
N
N
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003731:Dental Caries / Q000188:drug therapy / Q000382:microbiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005936:Glucans / Q000378:metabolism; D005964:Glucosyltransferases; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D011485:Protein Binding; D011506:Proteins / Q000037:antagonists & inhibitors*; D013295:Streptococcus mutans / Q000201:enzymology*; D013395:Sucrose / Q000378:metabolism
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005936:Glucans; D010446:Peptide Fragments; D011506:Proteins; C067118:GTF-I protein, Streptococcus; D013395:Sucrose; D005964:Glucosyltransferases; C489364:glucosyltransferase I