[Preparation and amplification of cDNAs from a single cell].
OBJECTIVE
To set up the technique by which gene expression analysis could be done from a few cells so as to resolve the challenging problem between increasingly needs of cellular gene expression pattern analysis and the difficulty of obtaining homogeneous cell materials in some cases.


METHODS
Pick up a single cell to do reverse transcription directly, and then amplify cDNA in sequence-independent manner by using a single primer containing oligo (dT) and test the representation of cDNA.


RESULTS
Representatively amplified cDNAs from a single HL-60 cell.


CONCLUSIONS
Producing whole cellular cDNAs probe from samples as small as a single cell could provide an ideal way to analyze differential gene expression in heterogeneous highly differentiating system and to establish cell type/tissue type specific gene expression profiles.
Liu
G
G
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical Collegy, Beijing, 100021 P.R.China. wumin@moon.cicams.ac.cn
Ferrari
N
N
Levi
G
G
Wu
M
M
D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification*; D018922:HL-60 Cells; D006801:Humans; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary