title: The relation between the number of PBSC and number of leukaphereses in children.
abstract: Although PBSC transplantation has an advantage over BM transplantation in that fewer burdens are placed on the patient at the time of stem cell collection, the number of collected cells decreases when leukapheresis is done repeatedly. We examined the relation between the number of times leukapheresis is performed and the number of mononuclear cells (MNC), CD34+ cells, and colony-forming unit-granulocyte-macrophages (CFU-GM) collected. The percentage of CD34+ cells was measured by flow cytometry and the number of CFU-GM was measured by a progenitor assay. The number of cells collected was significantly decreased by the third collection. Therefore, to secure enough cells for transplantation, leukapheresis ideally should be performed no more than twice if PBSC collection is to be efficient.
authors.affiliation: Department of Pediatrics, Kawasaki Medical School, Matsushima, Kurashiki, Okayama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kajiume
authors.forename: S
authors.initials: S
authors.lastname: Yoshimi
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: N
authors.initials: N
authors.lastname: Kataoka
mesh_terms: D000293:Adolescent; D000328:Adult; D001772:Blood Cell Count; D002648:Child; D002675:Child, Preschool; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D007937:Leukapheresis; D008297:Male; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous