Toward an in vitro test for the diagnosis of allergy to penicillins. Synthesis, characterization, and use of beta-lactam and beta-lactam metabolite poly-L-lysines which recognize human IgE antibodies.
Conjugates of poly-L-lysine (PLL) containing a penicillin or a penicilloyl residue were prepared and characterized by 1H NMR and by size-exclusion (SE) HPLC. These conjugates were used in a radio allergo sorbent tests (RAST) test for the determination of allergy toward beta-lactams. The chemiometric evaluation of the data indicates that allergy to amoxycillin is different from allergy to the other beta-lactams tested. Furthermore, careful chemical characterization of the conjugates appears to be crucial to obtain meaningful information from the RAST data.
Bolzacchini
E
E
Dipartimento di Sicenze dell'Ambiente e del Territorio, Universita' di Milano, Italy.
Consonni
V
V
Gramatica
P
P
Meinardi
S
S
Orlandi
M
M
Rindone
B
B
Todeschini
R
R
Blanca
M
M
Mayorga
C
C
D002851:Chromatography, High Pressure Liquid; D003888:Desensitization, Immunologic; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D010406:Penicillins / Q000009:adverse effects / Q000276:immunology*; D011107:Polylysine / Q000737:chemistry / Q000276:immunology*; D011852:Radioallergosorbent Test; D047090:beta-Lactams / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*
D010406:Penicillins; D047090:beta-Lactams; D011107:Polylysine; D007073:Immunoglobulin E