Sertoli cells decrease microglial response and increase engraftment of human hNT neurons in the hemiparkinsonian rat striatum.
Sertoli cells (SCs) provide immune protection and nutritive support to the developing germ cells in the testis. Sertoli cells have also been shown to provide immune protection to islets transplanted outside the testes. In this study, the ability of these cells to diminish the infiltration/activation of microglia into a neural graft implanted in the lesioned striatum of a hemiparkinsonian rat was investigated. Human neuron-like cells (hNT neurons) were implanted either alone or in combination with rat SCs. Three months later, the animals were sacrificed and immunohistochemistry was performed to determine the survival of the xenografted neurons as well as microglial infiltration/activation. Cotransplantation of the SCs with the hNT neurons increased graft survival and was associated with an increase in graft size. Furthermore, there were fewer microglia present in the grafted tissue of the cotransplantation groups. These results show that SCs retain their immunosuppressive ability even within the brain. As immune responses to grafted neural tissue within the central nervous system become better understood, this ability of the SCs to provide localized immunosuppression to the transplanted tissue may become more important. This is particularly true as the search for alternative sources of neural tissue to treat neurodegenerative diseases expands to encompass other species.
Willing
A E
AE
Department of Neurosurgery, University of South Florida College of Medicine, Tampa 33612, USA.
Sudberry
J J
JJ
Othberg
A I
AI
Saporta
S
S
Poulos
S G
SG
Cameron
D F
DF
Freeman
T B
TB
Sanberg
P R
PR
D000818:Animals; D000911:Antibodies, Monoclonal; D003176:Complement C3 / Q000378:metabolism; D003342:Corpus Striatum / Q000601:surgery*; D006085:Graft Survival / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D017628:Microglia / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000637:transplantation*; D010302:Parkinson Disease, Secondary / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011951:Receptors, Complement / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation
D000911:Antibodies, Monoclonal; D003176:Complement C3; D000949:Histocompatibility Antigens Class II; D011951:Receptors, Complement