Abundance and distribution of bacteria carrying sltII gene in natural river water.
Direct in situ PCR with HNPP/Fast Red TR was used to enumerate bacteria carrying the sltII gene in river water. By direct in situ PCR with a sltII-specific EVS primer, 10(2)-10(5) cells ml-1 of bacteria carrying the sltII gene were detected from all sampling sites, except the site nearest to the source of the river, while 10(2)-10(4) cells ml-1 of Escherichia coli O157:H7 were detected using a direct fluorescent antibody staining method. These results indicate that such bacteria are commonly distributed in natural river water. Direct in situ PCR with HNPP/Fast Red TR is a useful tool for detecting cells carrying specific genes, such as verotoxin-producing bacteria in natural environments.
Kurokawa
K
K
Genome Information Research Center, Graduate School of Pharmacy, Osaka University, Japan.
Tani
K
K
Ogawa
M
M
Nasu
M
M
D001427:Bacterial Toxins / Q000235:genetics*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005618:Fresh Water / Q000382:microbiology*; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods*; D012621:Seasons; D012722:Sewage / Q000382:microbiology; D022641:Shiga Toxin 2; D014876:Water Pollution
D001427:Bacterial Toxins; D012722:Sewage; D022641:Shiga Toxin 2