title: Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes.
abstract: We have studied the induction of chromosomal aberrations in human lymphocytes exposed in G0 to X rays or carbon ions. Aberrations were analyzed in G0, G1, G2 or M phase. Analysis during the interphase was performed by chemically induced premature chromosome condensation, which allows scoring of aberrations in G1, G2 and M phase; fusion-induced premature chromosome condensation was used to analyze the damage in G0 cells after incubation for repair; M-phase cells were obtained by conventional Colcemid block. Aberrations were scored by Giemsa staining or fluorescence in situ hybridization (chromosomes 2 and 4). Similar yields of fragments were observed in G1 and G2 phase, but lower yields were scored in metaphase. The frequency of chromosomal exchanges was similar in G0 (after repair), G2 and M phase for cells exposed to X rays, while a lower frequency of exchanges was observed in M phase when lymphocytes were irradiated with high-LET carbon ions. The results suggest that radiation-induced G2-phase block is associated with unrejoined chromosome fragments induced by radiation exposure during G0.
authors.affiliation: Space and Particle Radiation Group, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Durante
authors.forename: Y
authors.initials: Y
authors.lastname: Furusawa
authors.forename: H
authors.initials: H
authors.lastname: Majima
authors.forename: T
authors.initials: T
authors.lastname: Kawata
authors.forename: E
authors.initials: E
authors.lastname: Gotoh
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D016195:G2 Phase / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D008387:Marine Toxins; D010080:Oxazoles / Q000494:pharmacology
chemical_list: D008387:Marine Toxins; D010080:Oxazoles; C059041:calyculin A