Systemic suppression of human peripheral blood phagocytic leukocytes after whole-body UVB irradiation.
We examined systemic effects of whole-body UVB irradiation on human peripheral blood phagocytes. We found that 24 h after a single erythemal dose of UVB radiation two phagocyte functions, adhesion and phagocytosis, were reduced by 50%. This functional suppression was accompanied by a significant decrease in the expression of complement receptors (CR1 and CR3) and IgG Fc receptors (FcRII and FcRIII). The greatest reduction (47%) was observed in CR3, which is important for both adhesion and phagocytosis. A kinetic analysis showed that both CR1 and CR3 levels started to decrease 15 min after the UVB exposure, reaching the lowest levels at 4.5- and 24-h time points, respectively. The down-modulation of CRs after whole-body UVB exposure was not due to a defective receptor synthesis or translocation from internal stores to plasma membrane because the maximal CR levels in stimulated cells were not affected by UVB. No change in the serum soluble ICAM-1 was detected after UVB, which rules out CD1 1b epitope masking by sICAM-1. UVB did not release low-receptor-density myeloid progenitor cells from storage pools into circulation. Interleukin 10, a mediator of UVB-induced immunosuppression, was unable to modulate CR expression in vitro. When seven suberythemal whole-body UVB exposures were given repeatedly within 2 weeks, a significant decrease in CR expression was seen, which was greatest after three irradiations. Our data suggest that an exposure to UVB has systemic effects in humans which, possibly due to the down-modulation of preexisting cell-surface receptors, suppress some important functions of circulating phagocytic cells.
Leino
L
L
Department of Clinical Chemistry, University of Turku, Finland. leino@draco.se.astra.com
Saarinen
K
K
Kivistö
K
K
Koulu
L
L
Jansen
C T
CT
Punnonen
K
K
D000328:Adult; D002448:Cell Adhesion / Q000276:immunology / Q000528:radiation effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000528:radiation effects; D006801:Humans; D007165:Immunosuppression Therapy / Q000009:adverse effects*; D016753:Interleukin-10 / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000528:radiation effects*; D010587:Phagocytosis / Q000276:immunology / Q000528:radiation effects; D017463:Receptors, Complement 3b / Q000096:biosynthesis; D017452:Receptors, IgG / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014916:Whole-Body Irradiation / Q000009:adverse effects*
D016177:Macrophage-1 Antigen; D017463:Receptors, Complement 3b; D017452:Receptors, IgG; D011994:Recombinant Proteins; D016753:Interleukin-10