Suppression of the neoplastic phenotype by transfection of phospholipase C beta 3 to neuroendocrine tumor cells.
The expression of phospholipase C beta 3 (PLCB3) is low or absent in several neuroendocrine neoplasias. To investigate the role of PLCB3 in the neuroendocrine tumorigenesis, we transfected a PLCB3 construct to three neuroendocrine tumor cell lines with a low PLCB3 expression. The growth rate and tumorigenicity were assessed in vitro by [3H]thymidine incorporation and cell counting, in vivo, by xenografting to nude mice. In vitro, PLCB3 expressing clones showed a significant growth inhibition. The tumor weight was reduced for one of the two xenografted PLCB3-transfected cell lines and in both, a reduced number of proliferating (Ki-67 positive) cells was observed. This study implies an essential role for PLCB3 in the neuroendocrine tumorigenesis.
Stålberg
P
P
Department of Internal Medicine, University Hospital, Uppsala, Sweden.
Wang
S
S
Larsson
C
C
Weber
G
G
Oberg
K
K
Gobl
A
A
Skogseid
B
B
D000818:Animals; D002455:Cell Division; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018358:Neuroendocrine Tumors / Q000201:enzymology* / Q000209:etiology; D010641:Phenotype; D054799:Phospholipase C beta; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000096:biosynthesis* / Q000235:genetics
D007527:Isoenzymes; D019394:Ki-67 Antigen; D010738:Type C Phospholipases; C518039:PLCB3 protein, human; D054799:Phospholipase C beta; C518040:Plcb3 protein, mouse; C518041:Plcb3 protein, rat