The expression of peripheral benzodiazepine receptors in human skin: the relationship with epidermal cell differentiation.
The peripheral benzodiazepine receptor (PBR) is a protein of mitochondrial outer membranes utilizing porphyrins as endogenous ligands. PBR is part of a heteromeric receptor complex involved in the formation of mitochondrial permeability transition pores and in the early events of apoptosis. PBR may function as an oxygen-dependent signal generator; recent data indicate that these receptors may preserve the mitochondria of haematopoietic cell lines from damage caused by oxygen radicals. To identify PBRs in human skin, we used a specific monoclonal antibody directed against the C-terminus fragment of the human receptor. PBR immunoreactivity was found in keratinocytes, Langerhans cells, hair follicles and dermal vascular endothelial cells. Interestingly, confocal microscopic examination of skin sections revealed that PBR expression was strongly upregulated in the superficial differentiated layers of the epidermis. Ultrastructurally, PBRs were distributed throughout the cytoplasm but were selectively expressed on the mitochondrial membranes of epidermal cells. The elevated level of PBRs in the spinous layer was not associated with an increased number of mitochondria nor with an increased amount of mRNA as assessed by in situ hybridization on microautoradiographed skin sections. The present work provides, for the first time, evidence of PBR immunoreactivity in human skin. This mitochondrial receptor may modulate apoptosis in the epidermis; its increased expression in differentiated epidermal layers may represent a novel mechanism of natural skin protection against free radical damage generated by ultraviolet exposure.
Stoebner
P E
PE
Department of Dermatology-Allergology-Photobiology, Hôpital St-Eloi, 2 Avenue Bertin Sans, 34295 Montpellier, Cedex 05, France.
Carayon
P
P
Penarier
G
G
Fréchin
N
N
Barnéon
G
G
Casellas
P
P
Cano
J P
JP
Meynadier
J
J
Meunier
L
L
D000328:Adult; D017209:Apoptosis; D002454:Cell Differentiation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006801:Humans; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000201:enzymology; D011963:Receptors, GABA-A / Q000032:analysis* / Q000378:metabolism
D011963:Receptors, GABA-A