Association of plasmid integrative J7W-1 prophage with Bacillus thuringiensis strains.
A region homologous to the genome of plasmid integrative phage J7W-1 was detected in the largest plasmid in 3 out of 22 type strains of Bacillus thuringiensis, dendrolimus (DEN), aizawai (AIZ) and indiana (IND). Phage induction by ethidium bromide observed particularly in the J7W-1 lysogen was identified in DEN and IND but not AIZ strains. The morphology of the phage induced in DEN and IND strains was identical to J7W-1, but the phage production in IND strain was lower as compared to the J7W-1 lysogen. Although the restriction analysis indicated that the prophage in DEN strain possessed a complete J7W-1 genome, modification and/or deletion had presumably occurred in AIZ and IND strains.
Kanda
K
K
Department of Applied Biological Sciences, Saga University, Japan.
Kitajima
Y
Y
Moriyama
Y
Y
Kato
F
F
Murata
A
A
D017103:Bacillus Phages / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D001413:Bacillus thuringiensis / Q000821:virology*; D004996:Ethidium / Q000494:pharmacology; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics; D014775:Virus Activation / Q000187:drug effects; D016662:Virus Integration / Q000235:genetics
D004996:Ethidium