title: Adjuvanted outer membrane protein vaccine protects poultry against infection with Salmonella enteritidis.
abstract: The immunogenicity of a sonicated extract (SE) and of outer membrane proteins (OMP) of Salmonella enteritidis was tested in birds of about 8 weeks of age. The dose, route of vaccination and the adjuvant used varied in different groups of birds. Two vaccine doses with or without adjuvant were given parenterally or orally 3 weeks apart. OMP vaccines gave significantly higher antibody titres than SE vaccines, as indicated by ELISA. The vaccines adjuvanted with oil produced higher antibody titres than those without any adjuvant. A dose of 1 mg of vaccine produced higher antibody titres than 0.5 mg of vaccine. Adjuvanted vaccine given subcutaneously elicited higher antibody responses than oral vaccines given without adjuvant. The birds were challenged with virulent S. enteritidis organisms at the end of the second week after a booster dose. None of the birds given 1 mg of OMP vaccine subcutaneously shed the organisms when tested by culturing cloacal swabs, although a few birds vaccinated with 0.5 mg of OMP vaccine did so. In general, adjuvanted OMP vaccines gave better protection than SE vaccines.
authors.affiliation: National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, Bareilly (UP).
authors.forename: M
authors.initials: M
authors.lastname: Meenakshi
authors.forename: C S
authors.initials: CS
authors.lastname: Bakshi
authors.forename: G
authors.initials: G
authors.lastname: Butchaiah
authors.forename: M P
authors.initials: MP
authors.lastname: Bansal
authors.forename: M Z
authors.initials: MZ
authors.lastname: Siddiqui
authors.forename: V P
authors.initials: VP
authors.lastname: Singh
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines