The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5.
Snf/Swi, a nucleosome remodeling complex, is important for overcoming nucleosome-mediated repression of transcription in Saccharomyces cerevisiae. We have addressed the mechanism by which Snf/Swi controls transcription in vivo of an Snf/Swi-dependent promoter, that of the SUC2 gene. By single-cell analysis, our results show that Snf/Swi is required for activated levels of SUC2 expression in every cell of a population. In addition, Snf/Swi is required for maintenance of SUC2 transcription, suggesting that continuous chromatin remodeling is necessary to maintain an active transcriptional state. Finally, Snf/Swi and Gcn5, a histone acetyltransferase, have partially redundant roles in the control of SUC2 transcription, suggesting a functional overlap between two different mechanisms believed to overcome repression by nucleosomes, nucleosome remodeling and histone acetylation.
Sudarsanam
P
P
Department of Genetics, Harvard Medical School, Boston, 200 Longwood Avenue, MA 02115, USA.
Cao
Y
Y
Wu
L
L
Laurent
B C
BC
Winston
F
F
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins; D051548:Histone Acetyltransferases; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D026901:Membrane Transport Proteins; D008957:Models, Genetic; D008968:Molecular Conformation; D009154:Mutation; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D013698:Templates, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins; D051548:Histone Acetyltransferases; D008164:Luminescent Proteins; D026901:Membrane Transport Proteins; D009707:Nucleosomes; D010940:Plant Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C078562:SWI1 protein, S cerevisiae; C078729:sucrose transport protein, plant; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C071570:SNF1-related protein kinases