title: GelStar nucleic acid gel stain: high sensitivity detection in gels.
abstract: GelStar nucleic acid gel stain can be used for sensitive fluorescent detection of both double-stranded (ds) and single-stranded (ss) DNAs, oligonucleotides and RNA in gels. The stain can be added to agarose gels at casting for immediate imaging after electrophoresis or can be used after electrophoresis with both agarose and acrylamide gels. GelStar stain is highly fluorescent only when bound to nucleic acids thus giving superior signal-to-noise ratios and obviating the need to destain the gel. The detection limits of GelStar strain are 20 pg for dsDNA, 25 pg for ssDNA and 10 ng for native or glyoxal-treated RNA.
authors.affiliation: FMC BioProducts, Rockland, ME, USA.
authors.forename: H W
authors.initials: HW
authors.lastname: White
authors.forename: N B
authors.initials: NB
authors.lastname: Vartak
authors.forename: T G
authors.initials: TG
authors.lastname: Burland
authors.forename: F P
authors.initials: FP
authors.lastname: Curtis
authors.forename: N
authors.initials: N
authors.lastname: Kusukawa
mesh_terms: D001709:Biotechnology; D004396:Coloring Agents; D004252:DNA Mutational Analysis; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes; D005782:Gels; D009696:Nucleic Acids / Q000032:analysis*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D005782:Gels; D009696:Nucleic Acids; D004996:Ethidium