title: Water-soluble 2,3,7,8-tetrachlorodibenzo-p-dioxin complex with human alpha-fetoprotein: properties, toxicity in vivo and antitumor activity in vitro.
abstract: The conditions for the formation of a non-covalent complex between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the human transport fetal protein, alpha-fetoprotein (AFP), have been studied. TCDD has been shown to form a stable complex with AFP in a 2:1 (TCDD:AFP) ratio. The apparent solubility of TCDD in water increases 10(5)-fold after complex formation. The toxicity of the TCDD:AFP complex injected into mice by the intravenous route is comparable with that of free TCDD administered in oil solution per os. The complex manifests very much higher toxicity (200-1400 times) against human tumor cells (CEM, MCF-7, HepG2) in vitro and surpasses TCDD in selectivity. AFP may facilitate TCDD transport in embryonic tissues and enhance its embryotoxic and teratogenic effects.
authors.affiliation: Moscow Research Institute of Medical Ecology, Russia. sergsev@aha.ru
authors.forename: A I
authors.initials: AI
authors.lastname: Sotnichenko
authors.forename: S E
authors.initials: SE
authors.lastname: Severin
authors.forename: G A
authors.initials: GA
authors.lastname: Posypanova
authors.forename: N B
authors.initials: NB
authors.lastname: Feldman
authors.forename: M I
authors.initials: MI
authors.lastname: Grigor'ev
authors.forename: E S
authors.initials: ES
authors.lastname: Severin
authors.forename: R V
authors.initials: RV
authors.lastname: Petrov
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D006801:Humans; D007275:Injections, Intravenous; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000737:chemistry* / Q000633:toxicity; D012995:Solubility; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000072317:Polychlorinated Dibenzodioxins; D000509:alpha-Fetoproteins