title: Observations on photochemical fluorescence enhancement of the terbium(III)-sparfloxacin system.
abstract: Fluorescence of terbium(III) was sensitized when excited in the presence of sparfloxacin (SPFX) in the aqueous solution because a Tb(III)-SPFX complex was formed. The sensitized fluorescence was further enhanced when this system was exposed to 365 nm ultraviolet light. By the spectral properties and contrast experiments, it is proved that irradiation makes this system undergo photochemical reactions and a new terbium complex which is more favorable to the intramolecular energy transfer is formed. The mechanism of photochemical fluorescence enhancement of the Tb(III)-SPFX system is discussed and a new sensitive and selective photochemical fluorimetry for the determination of SPFX is established. Under the optimum conditions, the linear range is 1.0-50 x 10(-7) M for SPFX, the detection limit is 3.0 x 10(-9) M and the R.S.D. for 5.0 x 10(-7) M SPFX is 1.3% (n = 9). Without any pretreatment the recovery of SPFX in human urine was determined with satisfaction.
authors.affiliation: Department of Chemistry, Beijing Normal University, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fangtian
authors.forename: Z
authors.initials: Z
authors.lastname: Tieli
authors.forename: J
authors.initials: J
authors.lastname: Linpei
authors.forename: Z
authors.initials: Z
authors.lastname: Huichun
authors.forename: W
authors.initials: W
authors.lastname: Shubin
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000378:metabolism / Q000652:urine*; D000995:Antitubercular Agents / Q000737:chemistry* / Q000652:urine*; D002138:Calibration; D005453:Fluorescence; D024841:Fluoroquinolones; D006801:Humans; D010777:Photochemistry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods; D013725:Terbium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D000995:Antitubercular Agents; D024841:Fluoroquinolones; D013725:Terbium; C061363:sparfloxacin