Inhibition of focus formation of transformed cloned cells by contact with non-transformed BALB/c 3T3 A31-1-1 cells.
When transformed cells were co-cultured with various densities of non-transformed BALB/c 3T3 A31-1-1 cells, the number of transformed cell foci decreased as the density of the A31-1-1 cells was increased. Under the condition of separate co-cultivation in which transformed cloned cells could not make contact with A31-1-1 cells, no inhibitory effect was induced. We examined with a dye-transfer assay the formation of heterologous gap-junctional intercellular communication (GJIC), links between the transformed cells and A31-1-1 cells before and after focus formation. Heterologous GJIC was observed almost always before, but almost never after, focus formation. Using time-lapse photography to record the fate of transformed cloned cells that did not form foci in the co-cultivation, it was noted that most of them were living, but did not proliferate. These results suggested that focus formation of transformed cloned cells was inhibited by contact with non-transformed A31-1-1 cells.
Sakamoto
Y
Y
Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
Takeda
Y
Y
Takagi
H
H
Tsuchiya
T
T
Shoji
A
A
Miyazaki
K
K
Umeda
M
M
D016475:3T3 Cells / Q000166:cytology; D000818:Animals; D002450:Cell Communication; D002452:Cell Count; D016923:Cell Death; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D002999:Clone Cells / Q000166:cytology; D018920:Coculture Techniques; D003260:Contact Inhibition / Q000502:physiology*; D017629:Gap Junctions / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D018715:Microscopy, Video; D013995:Time; D014410:Tumor Stem Cell Assay