A missense mutation in the GHR gene of Cornell sex-linked dwarf chickens does not abolish serum GH binding.
Sex-linked dwarfism (SLD) in chickens is characterized by impaired growth despite normal or supranormal plasma growth hormone (GH) levels. This resistance to GH action is thought to be due to mutations of the GH receptor (GHR) gene that reduce or prevent GH binding to target sites. The genetic lesion causing GH resistance in Cornell SLD chickens is, however, not known. Previous studies have shown that hepatic GH-binding activity is abnormally low in these birds, yet the GHR gene is transcribed into a transcript of appropriate size and abundance. Point mutations or defects in translation could therefore account for the impaired GHR activity in this strain. These possibilities were addressed in the present study. A missense mutation resulting in the substitution of serine for the conserved phenylalanine was identified in the region of the GHR cDNA encoding the extracellular domain. Translation of this mutant transcript was indicated by the presence of GHR/GH-binding protein (GHBP)-immunoreactive proteins in liver (55, 70 and 100 kDa) and serum (70 kDa) of normal (K) and SLD birds. Radiolabelled GH did not, however, bind to the hepatic membranes of most SLD chickens. Serum GH-binding activity, in contrast, was readily detectable, although at significantly lower levels than in normal birds. The missense mutation in the SLD GHR gene may thus affect targeting of GHRs to hepatic plasma membranes.
Hull
K L
KL
Department of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
Marsh
J A
JA
Harvey
S
S
D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D004392:Dwarfism / Q000235:genetics*; D013006:Growth Hormone / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D020125:Mutation, Missense; D011485:Protein Binding; D011869:Radioligand Assay; D011986:Receptors, Somatotropin / Q000235:genetics*; D012730:Sex Chromosomes
D011986:Receptors, Somatotropin; D013006:Growth Hormone