title: A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains.
abstract: Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10(-7) per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri.
authors.affiliation: Center for Information Biology, National Institute of Genetics, Mishima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Okayama
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: J
authors.initials: J
authors.lastname: Takeda
authors.forename: R W
authors.initials: RW
authors.lastname: Davis
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Gojobori
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D007564:Japan; D008969:Molecular Sequence Data; D009154:Mutation; D009711:Nucleotides / Q000235:genetics*; D016415:Sequence Alignment; D012699:Serology; D012763:Shigella flexneri / Q000235:genetics; D013997:Time Factors; D014481:United States
chemical_list: D009711:Nucleotides