Using hippocampal slices to study how aging alters ion regulation in brain tissue.
Aging alters ion regulation in brain tissue. This article describes methods useful for studying such age-related changes in the rat hippocampal slice preparation. Topics considered include (a) selection of appropriate age groups of rats for aging studies, (b) a description of methods for preparing and maintaining hippocampal slices, (c) measurement of intracellular pH with the H+-sensitive dye carboxy-SNARF-1, and (d) measurement of extracellular pH and K+ with cation-selective microelectrodes.
Roberts
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Geriatric Research, Education, and Clinical Center, Miami VA Medical Center, Miami, Florida 33125, USA. roberts@neuron.med.miami.edu
D000375:Aging / Q000502:physiology*; D000818:Animals; D001578:Benzopyrans; D002138:Calibration; D004210:Dissection / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000254:growth & development / Q000502:physiology*; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D008839:Microelectrodes; D009284:Naphthols; D017966:Pyramidal Cells / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D012235:Rhodamines; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
D001578:Benzopyrans; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009284:Naphthols; D012235:Rhodamines; C068301:carboxy-seminaphthorhodaminefluoride