title: A novel method for predicting transmembrane segments in proteins based on a statistical analysis of the SwissProt database: the PRED-TMR algorithm.
abstract: We present a novel method that predicts transmembrane domains in proteins using solely information contained in the sequence itself. The PRED-TMR algorithm described, refines a standard hydrophobicity analysis with a detection of potential termini ('edges', starts and ends) of transmembrane regions. This allows one both to discard highly hydrophobic regions not delimited by clear start and end configurations and to confirm putative transmembrane segments not distinguishable by their hydrophobic composition. The accuracy obtained on a test set of 101 non-homologous transmembrane proteins with reliable topologies compares well with that of other popular existing methods. Only a slight decrease in prediction accuracy was observed when the algorithm was applied to all transmembrane proteins of the SwissProt database (release 35). A WWW server running the PRED-TMR algorithm is available at http://o2.db.uoa. gr/PRED-TMR/
authors.affiliation: Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Panepistimiopolis, Athens 15701, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Pasquier
authors.forename: V J
authors.initials: VJ
authors.lastname: Promponas
authors.forename: G A
authors.initials: GA
authors.lastname: Palaios
authors.forename: J S
authors.initials: JS
authors.lastname: Hamodrakas
authors.forename: S J
authors.initials: SJ
authors.lastname: Hamodrakas
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D019992:Databases as Topic; D020407:Internet; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D008565:Membrane Proteins