title: Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours.
abstract: The RCK gene is a target of the t(11;14)(q23;q32) chromosomal translocation observed in human B-cell lymphoma, and the overexpression of its protein (rck/p54) by the translocation was shown to cause malignant transformation. The rck/p54 protein belongs to the DEAD box protein/RNA helicase family, which has a variety of functions such as translation initiation, pre-mRNA splicing and ribosome assembly. The expression of rck p54 in colorectal adenocarcinoma cells was examined by immunohistochemistry and Western blot analysis. The rck/p54 protein was found to be overexpressed in tumour tissues resected from 13 (50%) out of 26 cases of colorectal adenocarcinomas and two out of two (100%) cases of colonic severe dysplastic adenomas. In view of activities of rck/p54 determined in other tissue types, we suggest that rck/p54 may contribute to the cell proliferation and carcinogenesis at the translational level in the development of colorectal tumours.
authors.affiliation: The Second Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: H
authors.initials: H
authors.lastname: Morikawa
authors.forename: I
authors.initials: I
authors.lastname: Hirata
authors.forename: M
authors.initials: M
authors.lastname: Shiozaki
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Maemura
authors.forename: T
authors.initials: T
authors.lastname: Nishikawa
authors.forename: M
authors.initials: M
authors.lastname: Niki
authors.forename: N
authors.initials: N
authors.lastname: Tanigawa
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
authors.forename: K
authors.initials: K
authors.lastname: Katsu
authors.forename: Y
authors.initials: Y
authors.lastname: Akao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D053487:DEAD-box RNA Helicases; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D012316:RNA Nucleotidyltransferases; C094683:DDX6 protein, human; D053487:DEAD-box RNA Helicases