Structure of an extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83.
The extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83 was found to be composed of D-glucose and D-galactose in a molar ratio of 2:3. The primary structure of the polysaccharide was shown by sugar analysis, methylation analysis, FABMS, partial acid hydrolysis and nuclear magnetic resonance (NMR) spectroscopy to consist of a pentasaccharide repeating unit having the following structure: -->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->6)-alpha-D-Galp-(1-->6 )-alpha-D -Glcp-(1-->3)-beta-D-Galf-(1-->
Vanhaverbeke
C
C
Centre de Recherches sur les Macromolécules Végétales, CNRS and Université Joseph Fourier, Grenoble, France.
Bosso
C
C
Colin-Morel
P
P
Gey
C
C
Gamar-Nourani
L
L
Blondeau
K
K
Simonet
J M
JM
Heyraud
A
A
D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D007778:Lactobacillus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D011134:Polysaccharides