Structure of VAT, a CDC48/p97 ATPase homologue from the archaeon Thermoplasma acidophilum as studied by electron tomography.
Valosine-containing protein-like ATPase from Thermoplasma acidophilum is a member of the superfamily of ATPases associated with a diversity of cellular activities and is closely related to CDC48 from yeast and p97 from higher eukaryotes and more distantly to N-ethylmaleimide-sensitive fusion protein. We have used electron tomography to obtain low-resolution (2-2.5 nm) three-dimensional maps of both the whole 500 kDa complex and the N-terminally truncated valosine-containing protein-like ATPase from T. acidophilum complex lacking the putative substrate binding domain.
Rockel
B
B
Max-Planck-Institut für Biochemie, Abteilung für molekulare Strukturbiologie, Martinsried, Germany.
Walz
J
J
Hegerl
R
R
Peters
J
J
Typke
D
D
Baumeister
W
W
D000251:Adenosine Triphosphatases / Q000648:ultrastructure*; D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D008854:Microscopy, Electron; D013822:Thermoplasma / Q000201:enzymology*; D000074405:Valosin Containing Protein
D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein; C105134:vat protein, Thermoplasma acidophilum