Experimental infection of pregnant cows with Bacillus licheniformis bacteria.
To study the abortifacient potential and fetoplacental tropism of Bacillus licheniformis bacteria, eight cows in the sixth to eighth month of gestation were inoculated intravenously either once (n = 4) or on four successive days (n = 4) with B. licheniformis at doses ranging from 10(9) to 10(12) colony-forming units. Cows were euthanatized and necropsied prior to abortion (n = 2), at the time of abortion (n = 2), or at calving (n = 4). Live-born calves (n = 5) were euthanatized immediately after delivery and necropsied. B. licheniformis was reisolated from placentomes/endometrium in six of eight (75%) cows and from one fetus aborted 43 days after inoculation. Lesions associated with B. licheniformis were restricted to the pregnant uterus, with the exception of one cow, which developed pneumonia. Necrosis in the fetal compartment of the placenta were present in three of four (75%) cows of both inoculation groups. Lesions were mainly restricted to fetal membranes and especially to the fetal side of the placentomes. Necrosis and diffuse neutrophil infiltrations of both villi and intervillous areas occurred in the fetal part of the placenta, and the placentomal interface was distended by bacteria, neutrophils, erythrocytes, and debris. Within trophoblasts, bacteria were located both free in the cytoplasm and in cytoplasmatic vesicles. Inflammation was present in three of eight (38%) calves. Placental and fetal lesions were similar to those found in cases of spontaneous abortions associated with B. licheniformis. The abortifacient potential of B. licheniformis and the tropism for the bovine placenta is demonstrated here for the first time.
Agerholm
J S
JS
Department of Pathology, Danish Veterinary Laboratory, Copenhagen, Denmark. jsa@svs.dk
Jensen
N E
NE
Dantzer
V
V
Jensen
H E
HE
Aarestrup
F M
FM
D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D000831:Animals, Newborn; D016863:Bacillaceae Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D001407:Bacillus / Q000472:pathogenicity*; D016470:Bacteremia / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005260:Female; D007150:Immunohistochemistry; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D010920:Placenta / Q000382:microbiology / Q000473:pathology; D011247:Pregnancy; D014327:Trophoblasts / Q000382:microbiology / Q000473:pathology