title: Bacterial surface display of an anti-pollutant antibody fragment.
abstract: A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia coli. Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate. Cells containing the PAL fusion grew with little cellular toxicity when compared with the control. In contrast, expression of anti-atrazine antibody fragments alone caused the cells to lyse after 4 h. The surface display of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, UK.
authors.forename: J K
authors.initials: JK
authors.lastname: Dhillon
authors.forename: P D
authors.initials: PD
authors.lastname: Drew
authors.forename: A J
authors.initials: AJ
authors.lastname: Porter
mesh_terms: D001280:Atrazine / Q000276:immunology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D007128:Immunoglobulin Fragments / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014873:Water Pollutants / Q000276:immunology*
chemical_list: D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins; D014873:Water Pollutants; D001280:Atrazine