Simultaneous production of catalase, glucose oxidase and gluconic acid by Aspergillus niger mutant.
The production of gluconic acid, extracellular glucose oxidase and catalase in submerged culture by a number of biochemical mutants has been evaluated. Optimization of stirrer speed, time cultivation and buffering action of some chemicals on glucose oxidase, catalase and gluconic acid production by the most active mutant, AM-11, grown in a 3-L glass bioreactor was investigated. Three hundred rpm appeared to be optimum to ensure good growth and best glucose oxidase production, but gluconic acid or catalase activity obtained maximal value at 500 or 900 rpm, respectively. Significant increase of dissolved oxygen concentration in culture (16-21%) and extracellular catalase activity were obtained when the traditional aeration was employed together with automatic dosed hydrogen peroxide.
Fiedurek
J
J
Department of Industrial Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
Gromada
A
A
Pielecki
J
J
D001234:Aspergillus niger / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors; D002374:Catalase / Q000096:biosynthesis*; D003470:Culture Media; D005898:Glass; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000096:biosynthesis*; D006861:Hydrogen Peroxide / Q000378:metabolism; D009154:Mutation; D010100:Oxygen / Q000494:pharmacology; D017640:Silicates; D012972:Sodium Hydroxide / Q000378:metabolism
D003470:Culture Media; D005942:Gluconates; D017640:Silicates; D012972:Sodium Hydroxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D005947:Glucose; C005144:pumice; C030691:gluconic acid; D010100:Oxygen