title: Sequence-ready physical map of the mouse chromosome 16 region with conserved synteny to the human velocardiofacial syndrome region on 22q11.2.
abstract: Proximal mouse Chromosome (Chr) 16 shows conserved synteny with human Chrs 16, 8, 22, and 3. The mouse Chr 16/human Chr 22 conserved synteny region includes the DiGeorge/Velocardiofacial syndrome region of human Chr 22q11.2. A physical map of the entire mouse Chr 16/human Chr 22 region of conserved synteny has been constructed to provide a substrate for gene discovery, genomic sequencing, and animal model development. A YAC contig was constructed that extends ca. 5.4 Mb from a region of conserved synteny with human Chr 8 at Prkdc through the region conserved with human Chr 3 at DVL3. Sixty-one markers including 37 genes are mapped with average marker spacing of 90 kb. Physical distance was determined across the 2.6-Mb region from D16Mit74 to Hira with YAC fragmentation. The central region from D16Jhu28 to Igl-C1 was converted into BAC and PAC clones, further refining the physical map and providing sequence-ready template. The gene content and borders of three blocks of conserved linkage between human Chr 22q11.2 mouse Chr 16 are refined.
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lund
authors.forename: B
authors.initials: B
authors.lastname: Roe
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: M
authors.initials: M
authors.lastname: Budarf
authors.forename: N
authors.initials: N
authors.lastname: Galili
authors.forename: R
authors.initials: R
authors.lastname: Riblet
authors.forename: R D
authors.initials: RD
authors.lastname: Miller
authors.forename: B S
authors.initials: BS
authors.lastname: Emanuel
authors.forename: R H
authors.initials: RH
authors.lastname: Reeves
mesh_terms: D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D002892:Chromosomes, Human, Pair 22; D004062:DiGeorge Syndrome / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D051379:Mice; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers