Relationship of serum N-acetyl-beta-glucosaminidase activity to oxidative stress in diabetes mellitus.
Serum N-acetyl-beta-glucosaminidase activity was evaluated in 40 Type 1 and 40 Type 2 diabetic patients and compared with parameters of diabetes control and oxidative stress. Significantly increased mean serum N-acetyl-beta-glucosaminidase activity was found in both groups of diabetic patients as compared with the corresponding group of healthy persons (p < 0.01). Oxidative stress measured by plasma malondialdehyde concentration was significantly higher in Type 2 than in Type 1 diabetic patients (p < 0.01) but in comparison with control subjects it was higher only in Type 2 diabetes. Plasma malondialdehyde concentration positively correlated with body mass index (r=0.77, p<0.001) and with serum N-acetyl-beta-glucosaminidase activities (r=0.57, p <0.001). Treatment of 10 Type 2 diabetic patients with antioxidant alpha-tocopherol caused a significant decrease in malondialdehyde concentration (p < 0.001) which was accompanied by a decrease of N-acetyl-beta-glucosaminidase activity (p < 0.01). We conclude that serum N-acetyl-beta-glucosaminidase activity may be influenced by oxidative stress which is more pronounced in Type 2 than in Type 1 diabetic patients.
Skrha
J
J
Department of Internal Medicine 3, Faculty of Medicine 1, Charles University, Prague, Czech Republic.
Hilgertová
J
J
D000118:Acetylglucosaminidase / Q000097:blood*; D000328:Adult; D015992:Body Mass Index; D003920:Diabetes Mellitus / Q000097:blood / Q000201:enzymology*; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood
D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000118:Acetylglucosaminidase