Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus).
The cDNA encoding the precursor of an aspartic proteinase from the flowers of the cardoon, Cynara cardunculus, was expressed in Pichia pastoris, and the recombinant, mature cyprosin that accumulated in the culture medium was purified and characterized. The resultant mixture of microheterogeneous forms was shown to consist of glycosylated heavy chains (34 or 32 kDa) plus associated light chains with molecular weights in the region of 14,000-18,000, resulting from excision of most, but not all, of the 104 residues contributed by the unique region known as the plant specific insert. SDS-polyacrylamide gel electrophoresis under non-reducing conditions indicated that disulfide bonding held the heavy and light chains together in the heterodimeric enzyme forms. In contrast, when a construct was expressed in which the nucleotides encoding the 104 residues of the plant specific insert were deleted, the inactive, unprocessed precursor form (procyprosin) accumulated, indicating that the plant-specific insert has a role in ensuring that the nascent polypeptide is folded properly and rendered capable of being activated to generate mature, active proteinase. Kinetic parameters were derived for the hydrolysis of a synthetic peptide substrate by wild-type, recombinant cyprosin at a variety of pH and temperature values and the subsite requirements of the enzyme were mapped using a systematic series of synthetic inhibitors. The significance is discussed of the susceptibility of cyprosin to inhibitors of human immunodeficiency virus proteinase and particularly of renin, some of which were found to have subnanomolar potencies against the plant enzyme.
White
P C
PC
School of Biosciences, Cardiff University, P. O. Box 911, Cardiff CF1 3US, Wales, United Kingdom.
Cordeiro
M C
MC
Arnold
D
D
Brodelius
P E
PE
Kay
J
J
D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D019659:Asteraceae / Q000201:enzymology*; D002402:Cathepsin D / Q000037:antagonists & inhibitors; D003001:Cloning, Molecular; D004792:Enzyme Precursors; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D010843:Pichia / Q000235:genetics; D010944:Plants / Q000201:enzymology; D017510:Protein Folding; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D004792:Enzyme Precursors; D009842:Oligopeptides; D011498:Protein Precursors; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; C087364:cyprosin; C058345:procathepsin D; D002402:Cathepsin D