A specific loss of C-series gangliosides in pancreas of streptozotocin-induced diabetic rats.
Gangliosides in pancreas, kidney, and liver tissues from streptozotocin-induced diabetic rats were analyzed by methods including thin-layer chromatographic (TLC) immunostaining with a specific monoclonal antibody to c-series gangliosides. In rats suffering diabetes for one month, the composition of major gangliosides in pancreatic tissue was almost identical to control, except for a slight increase in the content of GM3. Though c-series gangliosides such as GT3, GT2, GQ1c, and CP1c were expressed in normal pancreatic tissue, they were practically lost in pancreas of diabetic animals. A specific loss of c-series gangliosides was also observed in pancreatic tissue from rats suffering diabetes only for three days. While the composition of major gangliosides in the kidney did not change, streptozotocin-induced diabetic conditions brought about significant increases in contents of practically all major ganglioside species in liver tissue. No change was observed in the amount and composition of c-series gangliosides in both tissues. These results strongly suggest that c-series gangliosides are specifically localized in pancreatic B cells.
Saito
M
M
Department of Clinical Pharmacology and Therapeutics, University of Shizuoka School of Pharmaceutical Sciences, Japan. saitom@ys2.u-shizuoka-ken.ac.jp
Ito
M
M
Sugiyama
K
K
D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D002855:Chromatography, Thin Layer; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism*; D004195:Disease Models, Animal; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D005732:Gangliosides