A pyruvated mannose-specific xanthan lyase involved in xanthan degradation by Paenibacillus alginolyticus XL-1.
The xanthan-degrading bacterium Paenibacillus alginolyticus XL-1, isolated from soil, degrades approximately 28% of the xanthan molecule and appears to leave the backbone intact. Several xanthan-degrading enzymes were excreted during growth on xanthan, including xanthan lyase. Xanthan lyase production was induced by xanthan and inhibited by glucose and low-molecular-weight enzymatic degradation products from xanthan. A xanthan lyase with a molecular mass of 85 kDa and a pI of 7.9 was purified and characterized. The enzyme is specific for pyruvated mannosyl side chain residues and optimally active at pH 6.0 and 55 degrees C.
Ruijssenaars
H J
HJ
Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, 6700 EV Wageningen, The Netherlands. Harald.Ruijssenaars@imb.ftns.wau.nl
de Bont
J A
JA
Hartmans
S
S
D001407:Bacillus / Q000201:enzymology*; D001673:Biodegradation, Environmental; D002240:Carbohydrate Sequence; D019757:Carbon-Oxygen Lyases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D019757:Carbon-Oxygen Lyases; C115444:xanthan lyase; D008358:Mannose; C002563:xanthan gum