title: Assessment of cortical dysfunction in human strabismic amblyopia using magnetoencephalography (MEG).
abstract: The aim of this study was to use the technique of magnetoencephalography (MEG) to determine the effects of strabismic amblyopia on the processing of spatial information within the occipital cortex of humans. We recorded evoked magnetic responses to the onset of a chromatic (red/green) sinusoidal grating of periodicity 0.5-4.0 c deg-1 using a 19-channel SQUID-based neuromagnetometer. Evoked responses were recorded monocularly on six amblyopes and six normally-sighted controls, the stimuli being positioned near the fovea in the lower right visual field of each observer. For comparison, the spatial contrast sensitivity function (CSF) for the detection of chromatic gratings was measured for one amblyope and one control using a two alternate forced-choice psychophysical procedure. We chose red/green sinusoids as our stimuli because they evoke strong magnetic responses from the occipital cortex in adult humans (Fylan, Holliday, Singh, Anderson & Harding. (1997). Neuroimage, 6, 47-57). Magnetic field strength was plotted as a function of stimulus spatial frequency for each eye of each subject. Interocular differences were only evident within the amblyopic group: for stimuli of 1-2 c deg-1, the evoked responses had significantly longer latencies and reduced amplitudes through the amblyopic eye (P < 0.05). Importantly, the extent of the deficit was uncorrelated with either Snellen acuity or contrast sensitivity. Localization of the evoked responses was performed using a single equivalent current dipole model. Source localizations, for both normal and amblyopic subjects, were consistent with neural activity at the occipital pole near the V1/V2 border. We conclude that MEG is sensitive to the deficit in cortical processing associated with human amblyopia, and can be used to make quantitative neurophysiological measurements. The nature of the cortical deficit is discussed.
authors.affiliation: Department of Psychology, University of London, Egham, Surrey, UK. s.j.anderson@rhbnc.ac.uk
authors.forename: S J
authors.initials: SJ
authors.lastname: Anderson
authors.forename: I E
authors.initials: IE
authors.lastname: Holliday
authors.forename: G F
authors.initials: GF
authors.lastname: Harding
mesh_terms: D000328:Adult; D000550:Amblyopia / Q000503:physiopathology*; D003118:Color Perception / Q000502:physiology; D015350:Contrast Sensitivity / Q000502:physiology; D005074:Evoked Potentials, Visual; D005260:Female; D006801:Humans; D015225:Magnetoencephalography; D008297:Male; D008875:Middle Aged; D010364:Pattern Recognition, Visual / Q000502:physiology*; D011601:Psychophysics; D013285:Strabismus / Q000503:physiopathology*; D014793:Visual Cortex / Q000503:physiopathology*