title: The distribution of HIV-1 subtypes in Fukuoka, Japan.
abstract: To determine the incidence of human immunodeficiency virus type-1 (HIV-1) subtypes in Fukuoka, Japan, viruses from 41 HIV-1 infected individuals were subtyped. Subtyping by V3-loop enzyme-linked immunosorbent assay (ELISA) showed 31 of the 41 subjects as subtype B (MN type), one as subtype A, one as subtype C, and eight untypable. The subject infected with subtype C was identified as a foreigner; the subtype A subject was Japanese. A phylogenetic analysis of nucleic acid sequences from the env C2-V3 region was also conducted. Genetic subtyping was successful for 25 samples: 23 samples were determined as subtype B, one subtype A and one subtype E. One of the individuals infected with subtype B, as well as the subtype A and subtype E subjects, were not Japanese. This study indicated that subtype B (USA and European type) is still dominant among HIV-1 infections in Fukuoka. Further, no Japanese were subtype E positive, which is increasing in the Kanto region. It is notable, however, that subtype A and subtype C infections, which are rare in Japan, were found in Fukuoka, located far from the metropolitan area of Tokyo.
authors.affiliation: Fukuoka Institute of Health and Environmental Sciences, Department of General Medicine, Kyushu University Hospital, Dazaifu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Chijiwa
authors.forename: T
authors.initials: T
authors.lastname: Ishibashi
authors.forename: S
authors.initials: S
authors.lastname: Kashiwagi
authors.forename: R
authors.initials: R
authors.lastname: Mori
mesh_terms: D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015483:HIV Antibodies / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D004279:DNA, Viral; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120