Electrophoretic karyotyping and triazole susceptibility of Candida glabrata clinical isolates.
A series of 35 strains of Candida glabrata isolated from 29 subjects (5 AIDS patients and 24 HIV-seronegative individuals) were typed by electrophoretic karyotyping and tested for their susceptibilities to both fluconazole and itraconazole. Almost every individual harboured his/her own specific isolate (DNA type). Neither the source of isolation nor the patient's HIV status was associated with a given DNA type. Recurrences were generally due to the persistence of the same DNA type over time. Only 9% of the isolates showed reduced susceptibility to fluconazole (MIC > or = 8.0 microg/ml), while 43% of the isolates showed reduced susceptibility to itraconazole (MIC > or = 0.25 microg/ml) (P = 0.02). These data show that electrophoretic karyotyping is a useful technique for DNA typing of isolates of Candida glabrata. Care must be taken prior to initiation of antifungal therapy with either of these drugs.
Barchiesi
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Istituto di Malattie Infettive e Medicina Pubblica, Università degli Studi di Ancona, Ospedale Umberto I, Italy. cmalinf@popcsi.unian.it
Falconi Di Francesco
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Arzeni
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Caselli
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F
Gallo
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Scalise
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G
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000235:genetics*; D002177:Candidiasis / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis*; D004352:Drug Resistance, Microbial / Q000235:genetics; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D007621:Karyotyping; D008826:Microbial Sensitivity Tests
D000935:Antifungal Agents; D004271:DNA, Fungal; D017964:Itraconazole; D015725:Fluconazole