Isolation and partial characterisation of the Triton X-100 solubilised protein antigen from Mycobacterium tuberculosis.
This report describes extraction of a new native antigen fraction from Mycobacterium tuberculosis without massive degradation of proteins by Triton X-100. The Triton X-100 solubilised protein (TSP) antigen showed a characteristic antigen profile and reproducible extraction pattern. To characterise the nature of their composition, the TSP antigen was fractionated by Triton X-114 phase partitioning. The TSP antigen contained a variety of lipids and glycoconjugates as well as diverse proteins. Most proteins were partitioned into the aqueous phase during phase fractionation, whereas non-protein molecules and lipoproteins were recovered in the detergent phase. The lymphoproliferative responses to the TSP aqueous fraction in healthy tuberculin reactors were significantly higher than those to the purified protein derivative (PPD) and unfractionated TSP. In contrast, the antibody responses to TSP aqueous fraction in tuberculosis patients showed weak reactivity. This study suggests that the TSP aqueous fraction can be used as a T-cell antigen associated with protective immunity against tuberculosis.
Kim
Hwa-Jung
HJ
Department of Microbiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
Jo
Eun-Kyeong
EK
Department of Microbiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
Park
Jeong-Kyu
JK
Department of Microbiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
Lim
Jae-Hyun
JH
Department of Microbiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
Min
Dullei
D
Department of Microbiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
Paik
Tae-Hyun
TH
Department of Microbiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D003902:Detergents / Q000737:chemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D017830:Octoxynol / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility; D014373:Tuberculin / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D003902:Detergents; D007106:Immune Sera; D014373:Tuberculin; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40