Sodium-dependent uptake of [3H]scyllo-inositol by Tetrahymena: incorporation into phosphatidylinositol, phosphatidylinositol-linked glycans, and polyphosphoinositols.
[3H]Scyllo-inositol was taken up by Tetrahymena cells through a sodium-dependent pathway wherein unlabeled scyllo- and myo-inositol competed for uptake. d-Glucose was a competitor of [3H]myo-inositol uptake, but did not appear to compete for [3H]scyllo-inositol uptake. Transport of [3H]scyllo- and [3H]myo-inositol was inhibited when sodium was removed from the labeling buffer and by phlorizin, an inhibitor of sodium-dependent transporters. Cytochalasin B, an inhibitor of facilitated glucose transporters, had no significant effect on inositol transport. Internalized [3H]scyllo-inositol was readily incorporated intact into phosphatidylinositol, phosphatidylinositol-linked glycans, and polyphosphoinositols. Distribution of [3H]scyllo- and [3H]myo-inositol radioactivity into individual polyphosphoinositols was found to differ.
Ryals
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Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State 39762, USA. per@ra.msstate.edu
Kersting
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D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism*; D010695:Phlorhizin / Q000494:pharmacology; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D012964:Sodium / Q000502:physiology*; D013237:Stereoisomerism; D013768:Tetrahymena / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D017261:Glycosylphosphatidylinositols; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D014316:Tritium; C009217:scyllitol; D003571:Cytochalasin B; D007294:Inositol; D012964:Sodium; D010695:Phlorhizin