Molecular farming of industrial proteins from transgenic maize.
Recombinant egg white avidin and bacterial B-glucuronidase (GUS) from transgenic maize have been commercially produced. High levels of expression were obtained in seed by employing the ubiquitin promoter from maize. The recombinant proteins had activities that were indistinguishable from their native counterparts. We have illustrated that down-stream activities in the production of these recombinant proteins, such as stabilizing the germplasm and processing for purification, were accomplished without any major obstacles. Avidin (A8706) and GUS (G2035) are currently marketed by Sigma Chemical Co.
Hood
E E
EE
Department of Food Science and Human Nutrition, Iowa State University, Ames 50101, USA. eha105@aol.com
Kusnadi
A
A
Nikolov
Z
Z
Howard
J A
JA
D000595:Amino Acid Sequence; D001360:Avidin / Q000096:biosynthesis / Q000235:genetics; D004529:Egg White; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D001360:Avidin; D005966:Glucuronidase