title: Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor.
abstract: In order to isolate the unidentified autoantigens in autoimmune diabetes, a human pancreatic islet cDNA library was constructed and screened with the sera from the diabetic patients. From the library screening, one clone (DRS-1) that strongly reacted with the sera was isolated. Subsequent sequence analysis revealed that the clone was a novel cDNA related to the diazepam binding inhibitor. DRS-1 was expressed in most tissues including liver, lung, tonsil, and thymus, in addition to pancreatic islets. DRS-1 was in vitro translated and the recombinant DRS-1 protein was expressed in Escherichia coli and purified. The size of the in vitro translated or bacterially expressed DRS-1 protein was in agreement with the conceptually translated polypeptide of DRS-1 cDNA. Further studies are required to test whether or not DRS-1 is a new autoantigen in autoimmune diabetes.
authors.affiliation: Clinical Research Center, Samsung Biomedical Research Institute, Seoul, South Korea.
authors.forename: K
authors.initials: K
authors.lastname: Suk
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: D Y
authors.initials: DY
authors.lastname: Hwang
authors.forename: S H
authors.initials: SH
authors.lastname: Ihm
authors.forename: H J
authors.initials: HJ
authors.lastname: Yoo
authors.forename: M S
authors.initials: MS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D001324:Autoantigens / Q000235:genetics; D001483:Base Sequence; D019748:Carbon-Carbon Double Bond Isomerases; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics; D064006:Dodecenoyl-CoA Isomerase; D015723:Gene Library; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism; D007535:Isomerases; D008969:Molecular Sequence Data; D012313:RNA / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D001324:Autoantigens; D018076:DNA, Complementary; D011994:Recombinant Proteins; D012313:RNA; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase; C119738:ECI2 protein, human