Virus passage through track-etch membranes modified by salinity and a nonionic surfactant.
Why do viruses sometimes not pass through larger pores in track-etch filters? Increasing the salinity (0.8 to 160 mM Na+) decreased phiX174 and PRD1 passage through track-etch polycarbonate membranes (sodium dodecyl sulfate coated but not polyvinylpyrrolidone coated) and PRD1 passage through polyester membranes. Undiminished passage when 0.1% Tween 80 was added implied that nonionic virus adsorption occurred and indicated that high levels of salinity decreased virus passage by decreasing electrostatic repulsion that prevented adsorption.
Lytle
C D
CD
Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 20857, USA. cdl@cdrh.fda.gov
Routson
L B
LB
Jain
N B
NB
Myers
M R
MR
Green
B L
BL
D000327:Adsorption; D010584:Bacteriophage phi X 174 / Q000502:physiology*; D008567:Membranes, Artificial; D008851:Micropore Filters; D011076:Polycarboxylate Cement; D011091:Polyesters; D011136:Polysorbates / Q000494:pharmacology; D012483:Salmonella Phages / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology*; D055672:Static Electricity; D013499:Surface Properties; D013501:Surface-Active Agents / Q000494:pharmacology*
D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; D011091:Polyesters; D011136:Polysorbates; D013501:Surface-Active Agents; C005506:polycarbonate; D012965:Sodium Chloride