title: Molecular typing of Escherichia coli O157:H7 (H-) isolates from cattle in Japan.
abstract: A total of 77 Escherichia coli O157:H7 (H-) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx-2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157:H7 infection in Japan.
authors.affiliation: Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Akiba
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Sameshima
authors.forename: K
authors.initials: K
authors.lastname: Katsuda
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods; D022603:Shiga Toxins
chemical_list: D001427:Bacterial Toxins; D022603:Shiga Toxins