title: Interstrain variation in the human cytomegalovirus DNA polymerase sequence and its effect on genotypic diagnosis of antiviral drug resistance. Adult AIDS Clinical Trials Group CMV Laboratories.
abstract: The polymerase (pol) coding sequence was determined for 40 independent clinical cytomegalovirus isolates sensitive to ganciclovir and foscarnet. Sequence alignments showed >98% interstrain homology and amino acid variation in only 4% of the 1, 237 codons. Almost all variation occurred outside of conserved functional domains where resistance mutations have been identified.
authors.affiliation: VA Medical Center, Portland, Oregon 97201, USA. chous@ohsu.edu
authors.forename: S
authors.initials: S
authors.lastname: Chou
authors.forename: N S
authors.initials: NS
authors.lastname: Lurain
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
authors.forename: G Y
authors.initials: GY
authors.lastname: Cai
authors.forename: P L
authors.initials: PL
authors.lastname: Sharma
authors.forename: C S
authors.initials: CS
authors.lastname: Crumpacker
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D003062:Codon; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000235:genetics; D004352:Drug Resistance, Microbial; D017245:Foscarnet / Q000494:pharmacology; D015774:Ganciclovir / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D017386:Sequence Homology, Amino Acid
chemical_list: D000998:Antiviral Agents; D003062:Codon; D017245:Foscarnet; D004259:DNA-Directed DNA Polymerase; D015774:Ganciclovir