Herpes simplex virus chronically infected human T lymphocytes are susceptible to HIV-1 superinfection and support HIV-1 pseudotyping.
Peripheral blood lymphocytes (PBL) and CEM CD4+ T-cell line can be infected by herpes simplex virus-1 (HSV-1). CEM cells were characterized as a cellular model to study interactions occurring between HSV-1 and HIV-1. Virtually all cells were persistently infected by HSV-1 (CEM(HSV)) and expressed the latency associated transcripts, whereas only a fraction tested positive for HSV-antigens. CD4 and CXCR-4 expression and function were not affected in CEM(HSV) cells and no significant increase of deoxyribonucleotide pools was noticed. Superinfection of CEM(HSV) cells with HIV-1 led to a cell line chronically infected by both viruses (CEM(HSV/HSV)). Evidence was also obtained that this cell line can produce HIV-1 pseudotyped by HSV-1 envelope. These results may have important implications for a better understanding of AIDS pathogenesis.
Calistri
A
A
Institute of Microbiology of the University of Padova, Italy.
Parolin
C
C
Pizzato
M
M
Calvi
P
P
Giaretta
I
I
Palù
G
G
D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D003854:Deoxyribonucleotides / Q000378:metabolism; D005434:Flow Cytometry; D016679:Genome, Viral; D015726:Giant Cells / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D008934:Mitogens / Q000494:pharmacology; D009500:Neutralization Tests; D019718:Receptors, CXCR4 / Q000378:metabolism; D015163:Superinfection / Q000821:virology*; D014759:Viral Envelope Proteins / Q000502:physiology; D014764:Viral Proteins / Q000032:analysis; D019065:Virus Assembly; D017735:Virus Latency / Q000187:drug effects / Q000235:genetics
D015704:CD4 Antigens; D003854:Deoxyribonucleotides; D008934:Mitogens; D019718:Receptors, CXCR4; D014759:Viral Envelope Proteins; D014764:Viral Proteins