title: The origin of marginal zone B cells in the rat.
abstract: The marginal zone is a unique compartment that is only found in the spleen. Rat marginal zone B cells (MZ-B) can be distinguished from other B cells, e.g. recirculating follicular B cells (RF-B), by several phenotypic characteristics. Typically MZ-B cells are surface (s)IgMhi, sIgDlo and CD45R(B220)lo, whereas RF-B cells are sIgMlo, sIgDhi and CD45Rhi. In addition, MZ-B cells stain strongly with HIS57, a newly developed monoclonal antibody. The developmental pathway and origin of MZ-B cells are not exactly known. However, previous studies indicate that recirculating (i. e. thoracic duct) B cells can give rise to MZ-B cells. Here the origin of (naive) MZ-B cells was studied using adriamycin (doxorubicin)-induced B cell depletion. Using three-color flow cytometry and immunohistology we show that 2 days after a single i.v. injection of the anti-tumor drug adriamycin only RF-B cells can be detected, while all other B cell subpopulations are depleted, including all bone marrow precursor B cells. By studying the sequential reappearance of various B cell subsets and their precursors after adriamycin administration we show that MZ-B cells and the splenic marginal zone can be detected at a time point at which newly generated B cells (immature B cells) are not yet present. Given the observation that only RF-B cells were present at this time, we conclude that RF-B cells are the immediate MZ-B precursor cells.
authors.affiliation: Department of Histology and Cell Biology, University of Groningen, The Netherlands. p.m.dammers@med.rug.nl
authors.forename: P M
authors.initials: PM
authors.lastname: Dammers
authors.forename: N K
authors.initials: NK
authors.lastname: de Boer
authors.forename: G J
authors.initials: GJ
authors.lastname: Deenen
authors.forename: P
authors.initials: P
authors.lastname: Nieuwenhuis
authors.forename: F G
authors.initials: FG
authors.lastname: Kroese
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D004317:Doxorubicin; D005434:Flow Cytometry; D008214:Lymphocytes; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000166:cytology
chemical_list: D016328:NF-kappa B; D004317:Doxorubicin