Extracellular reduction of Cat1 free radical by transformed human hepatocytes.
Redox activities associated with plasma membranes of nonphagocytic animal and plant cells have been reported by several authors. However, the natural substrates, structure and biological role of these putative enzyme systems are not known. Data indicating extracellular reduction of a nitroxide free radical Cat1 (1-oxy-4-trimethylamine-2,2,6,6,tetramethyl-piperidine) by hepatocytes were thought to be artefactual. We report evidence in support of a notion that Cat1 as well as a tetrazolium salt, CTC (5-cyano-2,3-ditolyl tetrazolium chloride), are reduced extracellularly, probably at the cell surface, by human HepG2 hepatoma cells. These data provide evidence confirming the existence of a yet unidentified reducing activity associated with outer surface of plasma membranes of transformed human hepatocytes.
Bernas
T
T
Department of Biophysics, Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Dobrucki
J
J
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D015220:Calcium Channels; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D002374:Catalase / Q000378:metabolism*; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D050916:TRPV Cation Channels; D013777:Tetrazoles / Q000737:chemistry; D013778:Tetrazolium Salts
D015220:Calcium Channels; D005609:Free Radicals; D050916:TRPV Cation Channels; C120776:TRPV6 channel; D013777:Tetrazoles; D013778:Tetrazolium Salts; D009243:NAD; C075453:5-cyano-2,3-ditolyltetrazolium; D002731:4-Chloromercuribenzenesulfonate; D004317:Doxorubicin; D002374:Catalase; D001205:Ascorbic Acid