[The evaluation of the specific type of capsular polysaccharide of Cryptococcus neoformans as an immunogen and positive-control antigen].
The specific type of capsular polysaccharide of an autochthonous strain of Cryptococcus neoformans var. neoformans was obtained by using the method of selective precipitation with hexadimethrine bromide. The capsular polysaccharide was matched to lamb's erythrocytes and it was used as an immunogen in rabbits. Antibody titres of up to 1:32 were attained. Doble serial dilutions of the capsular polysaccharide were evaluated as positive control antigen by contraimmunoelectrophoresis, latex and ELISA techniques, showing biological activity.
Toraño Peraza
G T
GT
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Rodríguez Acebo
W
W
Martínez Machín
G
G
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis / Q000097:blood; D000946:Antigens, Fungal / Q000276:immunology* / Q000302:isolation & purification; D003455:Cryptococcus neoformans / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D007114:Immunization / Q000379:methods; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D012756:Sheep
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D011134:Polysaccharides