Proteome analysis of factor for inversion stimulation (Fis) overproduction in Escherichia coli.
The factor-for-inversion stimulation protein (Fis) is a global regulatory protein in Escherichia coli that activates ribosomal RNA (rRNA) transcription by binding to three upstream activation sites of the rRNA promoter and enhances transcription 5- to 10-fold in vivo. Fis overexpression results in different effects on cell growth depending on nutrient conditions. Differential proteome analysis of Fis-expressing cells shows ten protein spots corresponding to Fis overexpression in both rich (YT) and minimal (M9+glucose) media. Three of these spots have been identified as elongation factor TS, histidine-binding periplasmic protein precursor, and ketol-acid reductoisomerase.
Choe
L H
LH
School of Chemical Engineering, Cornell University, Ithaca, NY 14853-5201, USA.
Chen
W
W
Lee
K H
KH
D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D016601:RNA-Binding Proteins; C440502:integration host factor, E coli