title: Comparison of synthesis and antibacterial activity of temporin A.
abstract: Temporin A is a small, basic, highly hydrophobic, antibacterial peptide found in the skin of the European red frog, Rana temporaria. It was synthesized twice by the FastMoc solid phase method using amino acids protected at the N(alpha)-position with either 9-fluorenylmethoxycarbonyl or 2-(4-nitrophenylsulfonyl)ethoxycarbonyl. The syntheses of temporin A demonstrates the difference between 2-(4-nitrophenylsulfonyl)ethoxycarbonyl and 9-fluorenylmethoxycarbonyl amino acids. The purified peptide showed also antibacterial activity against clinically important gram-positive bacteria. It was found to have a moderately good activity against both methicillin resistant and sensitive strains of Staphylococcus aureus, but a weaker activity against vancomycin resistant strains of Enterococcus faecium.
authors.affiliation: Haartman Institute, Department of Virology, Helsinki University, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Harjunpää
authors.forename: P
authors.initials: P
authors.lastname: Kuusela
authors.forename: M T
authors.initials: MT
authors.lastname: Smoluch
authors.forename: J
authors.initials: J
authors.lastname: Silberring
authors.forename: H
authors.initials: H
authors.lastname: Lankinen
authors.forename: D
authors.initials: D
authors.lastname: Wade
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D016984:Enterococcus faecium / Q000187:drug effects*; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D011896:Rana temporaria; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides