Differential expression of two spermidine synthase genes during early fruit development and in vegetative tissues of pea.
Two cDNAs from young pea fruits coding for functional spermidine synthases (EC 2.5.1.16) were isolated. The corresponding genes were named psSPDSYN1 and psSPDSYN2. Both cDNAs complemented spe3delta gene when introduced into the Y480 strain of Saccharomyces cerevisiae, which is a null mutant for the spermidine synthase gene. psSPDSYN1 and psSPDSYN2 are regulated differentially. psSPDSYN1 is up-regulated early after fruit set whereas psSPDSYN2 is expressed later. Spermidine synthase activity was detected in pea ovaries, and correlates with the pattern of expression of psSPDSYN1. In the pea plant, psSPDSYN1 is highly expressed in actively growing tissues, whereas the highest level of psSPDSYN2 mRNA was detected in fully elongated stem.
Alabadí
D
D
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
Carbonell
J
J
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D018744:DNA, Plant / Q000032:analysis; D004790:Enzyme Induction / Q000187:drug effects; D005638:Fruit / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D005875:Gibberellins / Q000494:pharmacology; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D011436:Spermidine Synthase / Q000235:genetics*; D014018:Tissue Distribution
D018076:DNA, Complementary; D018744:DNA, Plant; D005875:Gibberellins; D012333:RNA, Messenger; C007842:gibberellic acid; D011436:Spermidine Synthase