Bioassay of biotin concentration with a Escherichia coli bio deletion mutant.
Biotin concentration was determined unequivocally with the E. coli bio mutant. The results demonstrate that this simple and efficient method can determine biotin concentration in the range of 10 pg to 50 ng/ml. The present method can also clearly distinguish biotin from its precursor and analog, dethiobiotin.
Hwang
S Y
SY
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Su
V
V
Farh
L
L
Shiuan
D
D
D001681:Biological Assay / Q000379:methods*; D001710:Biotin / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism*; D017353:Gene Deletion; D016296:Mutagenesis; D013997:Time Factors
D001710:Biotin