Establishment of a near-triploid human B-cell lymphoma cell line with t(14;18) and a p53 gene point mutation.
We report a rare large B-cell non-Hodgkin's lymphoma having a characteristic near-triploid cell population with add(17)(p22) and t(14;18)(q32;q21) translocation. We also established and characterized a new cell line (TK cell) derived from the present lymphoma. A codon 180 mutation (GAG --> GAT) in the p53 gene was detected. t(14;18)(q32;q21) was revealed juxtaposition of the bcl-2 and JH genes. Immunoprecipitation analyses of p53 and bcl-2 revealed that abnormality of the p53 protein and aberrant bcl-2 expression, which may protect cells from apoptosis, may be critical to the development of leukaemogenesis exhibiting near-triploid chromosomes.
Yamaguchi
H
H
Division of Haematology, Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
Inokuchi
K
K
Hanawa
H
H
Tanosaki
S
S
Shinozawa
I
I
Matuoka
H
H
Dan
K
K
D000368:Aged; D000369:Aged, 80 and over; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D007694:Killer Cells, Natural; D016393:Lymphoma, B-Cell / Q000235:genetics*; D011003:Ploidies; D017354:Point Mutation / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics*
D019253:Proto-Oncogene Proteins c-bcl-2