Aortic endothelial cells damaged by a nitric oxide donor and protected by flavonoids.
Cultured porcine aortic endothelial cells (PAEC) were exposed to four concentrations (0.00 mM - 5.00 mM) of 3-Morpholino-sydnonimine-hydrochloride (SIN-1, a nitric oxide donor). SIN-1 demonstrated a dose dependent cytotoxicity against PAEC as indicated by the thiobarbituric acid (TBA) assay. Morphologically and biochemically, the presence of selected flavonoids (morin, quercetin, or catechin) was shown to protect the PAEC from SIN-1 toxicity. Protection levels determined from the TBA assay were significant (p<0.05) for all flavonoids, with morin at 72+/-8%. Quercetin and catechin had comparable protective activities of 54+/-6% and 43+/-3%, respectively. This study supports the contention that SIN-1 is cytotoxic to PAEC and that antioxidants such as flavonoids may attenuate such toxicity.
Law
A
A
Department of Clinical Biochemistry, University of Toronto, and Toronto Hospital, Canada.
Wu
J
J
Zeng
L H
LH
Wu
T W
TW
D000818:Animals; D001011:Aorta / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D005419:Flavonoids / Q000494:pharmacology*; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000633:toxicity; D009336:Necrosis; D020030:Nitric Oxide Donors / Q000633:toxicity*; D013552:Swine
D005419:Flavonoids; D020030:Nitric Oxide Donors; C002385:linsidomine; D008981:Molsidomine