title: Infectivity of feline enteroepithelial stages of Toxoplasma gondii isolated by Percoll-density gradient centrifugation.
abstract: The infectivity of the feline enteroepithelial stages of Toxoplasma gondii isolated by Percoll-density gradient centrifugation was examined by the trypan blue dye exclusion method by assaying their penetration into feline fibroblast cells in vitro and by inoculation of the intestinal mucosa of cats. A large population of the parasites showed trypan blue dye exclusion activity. When feline fibroblast cells were inoculated with feline enteroepithelial stage parasites, no intracellular parasites were found 18 h post-inoculation. Kittens inoculated intraduodenally with 2 x 10(6) feline enteroepithelial stage parasites shed oocysts between 2 and 8 days post-inoculation. These results indicate that the isolated feline enteroepithelial stage parasites display infectivity towards enterocytes of cats and are capable of gametogenesis.
authors.affiliation: Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Omata
authors.forename: T
authors.initials: T
authors.lastname: Kawano
authors.forename: T
authors.initials: T
authors.lastname: Ohsawa
authors.forename: S
authors.initials: S
authors.lastname: Sugaya
authors.forename: M
authors.initials: M
authors.lastname: Satake
authors.forename: T
authors.initials: T
authors.lastname: Isamida
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: A
authors.initials: A
authors.lastname: Taka
authors.forename: K
authors.initials: K
authors.lastname: Miyazawa
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000981:Antiprotozoal Agents / Q000378:metabolism; D002371:Cat Diseases / Q000276:immunology* / Q000469:parasitology; D002415:Cats; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000662:veterinary; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013047:Specific Pathogen-Free Organisms; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000276:immunology*; D014343:Trypan Blue / Q000378:metabolism
chemical_list: D000913:Antibodies, Protozoan; D000981:Antiprotozoal Agents; D014343:Trypan Blue