[The determination of antibodies to the human immunodeficiency virus type 1 (HIV-1) in samples of dried blood on filter paper].
The possibility of detecting HIV-1 antibodies by an immunoblotting kit is studied in a panel of 125 known specimens of dried blood spotted on filter paper and their corresponding serum samples. No differences were observed in the patterns of bands with both types of samples or in the sensitivity and specificity, where 100% figures were attained, allowing to conclude that the blood specimen taken on filter paper may be used for the detection of HIV-1 antibodies by the DAVIH-BLOT system and may be kept at 4 degrees C during 30 weeks.
Pérez Guevara
M T
MT
Centro de Investigaciones Científicas, Defensa Civil, Laboratorio de Investigaciones del SIDA, San José de las Lajas, Cuba.
Rolo Gómez
F M
FM
Nibot Sánchez
C
C
Cruz Sui
O
O
Rodríguez
O
O
D015492:AIDS Serodiagnosis / Q000295:instrumentation / Q000379:methods; D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods; D005374:Filtration / Q000295:instrumentation; D015483:HIV Antibodies / Q000097:blood*; D006801:Humans; D010209:Paper; D012680:Sensitivity and Specificity
D015483:HIV Antibodies