[The excretion pattern and protective capacity in rabbits orally immunized with attenuated strains of Vibrio cholerae O1 biotype El Tor].
In order to study the excretion patterns, colonization and protective capacity of live attenuated strains of Vibrio cholerae O1. El Tor, rabbits were immunized in New Zealand with these strains and their corresponding parental strains. 2 doses were administered by the model of oral inoculation in adult rabbits. Rabbits were rotated 2 weeks after the second dose by the model of ligated intestine with highly virulent strains of V. cholerae O1 Ogawa and Inaba serotypes and O139 serogroup. It was proved that the genetically manipulated strains do not effect the excretion patterns when they are compared with their parental strains. It was observed in the challenge a decrease in the levels of colonization of virulent strains of both serotypes, not only among the rabbits immunized with the attenuated strains, but also among those immunized with the parental strains in comparison with control animals immunized with the strain of Escherichia coli K-12, which means that there was certain degree of protection. In the case of the animals challenged with the O139 strain it was demonstrated that the protection is specific for each serogroup, since in this case there was no reduction of the colonization.
García Sánchez
H M
HM
Centro de Investigación, Producción de Vacunas Y Sueros, Instituto Finlay, Ciudad de La Habana, Cuba.
García Imia
L
L
Oliva Hernández
R
R
Pérez Quiñoy
J L
JL
Cedré Marrero
B
B
Domínguez Iriarte
R
R
Benítez Robles
J
J
D000284:Administration, Oral; D000818:Animals; D002771:Cholera / Q000382:microbiology* / Q000517:prevention & control*; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D005243:Feces / Q000382:microbiology; D007114:Immunization / Q000379:methods*; D011817:Rabbits; D012703:Serotyping; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D014734:Vibrio cholerae / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity
D022121:Cholera Vaccines; D014613:Vaccines, Attenuated