Transcribed dinucleotide microsatellites and their associated genes from channel catfish Ictalurus punctatus.
The presence of trinucleotide microsatellites within genes is a well-known cause for a number of genetic diseases. However, the precise distribution of dinucleotide microsatellites within genes is less well documented. Here we report 15 unique cDNAs containing dinucleotide repeats from the channel catfish Ictalurus punctatus. Gene identities of nine of the 15 cDNAs were determined, of which three encode structural genes, and six encode regulatory proteins. Five cDNAs harbored dinucleotide repeats in the 5' untranslated region (5'-NTR), nine in the 3'-NTR, and one in the coding region. The presence of these transcribed dinucleotide repeats and their potential expansion in size within coding regions could lead to disruption of the original protein and/or formation of new genes by frame shift. The low number of dinucleotide repeats within coding regions suggests that they were strongly selected against. All the transcribed microsatellite loci examined were polymorphic making them useful for gene mapping in catfish.
Liu
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Z
Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, Alabama, 36849, USA. zliu@acesag.auburn.edu
Tan
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Li
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Dunham
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D000818:Animals; D017931:DNA Primers / Q000235:genetics; D016208:Databases, Factual; D018900:Dinucleotide Repeats / Q000235:genetics*; D015723:Gene Library; D007059:Ictaluridae / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010902:Pituitary Gland / Q000378:metabolism; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D017931:DNA Primers