Monitoring the genotoxicity of soil extracts from two heavily polluted sites in Prague using the Tradescantia stamen hair and micronucleus (MNC) assays.
We have taken soil samples from two sites in Prague, the capital of the Czech Republic, that are heavily polluted by motor vehicles. As a negative control, soil samples from a recreational site in Prague with no motor vehicle traffic were used. Soil samples from these sites were extracted with water or 5% dimethylsulphoxide (DMSO) for 24 h and cuttings of Tradescantia clone 4430 were immersed for 12 h at 25 degrees C in the extraction solutions. As a positive control Tradescantia plants have been treated with the promutagenic arylamine o-phenylenediamine at the same treatment conditions. None of the tested soil extractions significantly increased the frequency of somatic mutations in the stamen hair assay. By contrast, a 5% DMSO soil extract from one of the tested sites (entrance of the Letná tunnel) significantly increased the frequency of micronuclei (MNC) in the pollen mother tetrad cells. A repetition of the treatment 14 days later also resulted in an increase in the frequency of MNC, however the increase was not statistically significant. This study was conducted for the International Programme on Plant Bioassays.
Gichner
T
T
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, 160 00, Praha 6, Czech Republic. gichner@ueb.cas.cz
Velemínský
J
J
D018153:Czech Republic; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D015162:Micronucleus Tests; D018986:Motor Vehicles; D009153:Mutagens / Q000633:toxicity*; D010655:Phenylenediamines / Q000633:toxicity; D010944:Plants / Q000187:drug effects* / Q000235:genetics; D015203:Reproducibility of Results; D012989:Soil Pollutants / Q000633:toxicity*; D014504:Urban Health
D009153:Mutagens; D010655:Phenylenediamines; D012989:Soil Pollutants; C034193:1,2-diaminobenzene; D004121:Dimethyl Sulfoxide