title: Stabilization of polymer-based gene delivery systems.
abstract: To preserve the size and transfection potential of polymer-plasmid complexes. Freeze-drying and freeze-thawing were used for stabilization of these complexes. The concentration of the sugars is an important factor affecting both the size and transfection capability of the complexes after freeze-drying and freeze-thawing. However, the type of lyoprotectant (sugar) used is of minor importance. It is also shown that when damage to polymer-plasmid complexes occurs, it results from the drying process but is not due to the freezing step.
authors.affiliation: Research School Groningen-Utrecht Institute for Drug Exploration (GUIDE), Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80.082, 3508 TB, Utrecht, The Netherlands.
authors.forename: J Y
authors.initials: JY
authors.lastname: Cherng
authors.forename: P
authors.initials: P
authors.lastname: vd Wetering
authors.forename: H
authors.initials: H
authors.lastname: Talsma
authors.forename: D J
authors.initials: DJ
authors.lastname: Crommelin
authors.forename: W E
authors.initials: WE
authors.lastname: Hennink
mesh_terms: D005612:Freeze Drying; D015316:Genetic Therapy; D008320:Maltose / Q000494:pharmacology; D010957:Plasmids; D011108:Polymers; D013395:Sucrose / Q000494:pharmacology; D014162:Transfection; D014199:Trehalose / Q000494:pharmacology
chemical_list: D011108:Polymers; D013395:Sucrose; D008320:Maltose; D014199:Trehalose