Increased uncoupling protein2 mRNA in white adipose tissue, and decrease in leptin, visceral fat, blood glucose, and cholesterol in KK-Ay mice fed with eicosapentaenoic and docosahexaenoic acids in addition to linolenic acid.
The effects of n-3 polyunsaturated fatty acids (n-3PUFA) on obesity and diabetes were examined using KK-Ay mice fed with perilla oil (P), soybean oil (S), or lard (L), and those containing 30% fish oil (PF, SF, or LF), containing eicosapentaenoic acid (EPA = 9.9%) and docosahexaenoic acid (DHA = 18.0%). Perilla oil contained the largest proportion of linolenic acid (LNA = 61.9%). Computerized tomography (CT) scans showed narrower areas of visceral fat in the abdominal cross sections of groups given fish oil (PF, SF, and LF) and lower leptin levels (p < 0.05-p < 0.001) compared with controls (P, S, and L), without significant changes in energy intake and body weight. The highest plasma n-3PUFA content (21.31 +/- 0.35%) was attained with PF. This group contained 2.6-fold more plasma DHA (p < 0.001), and expressed 2.7-fold more UCP2 mRNA in white adipose tissue (p < 0.01) than in the P group. The epididymal fat pad (p < 0.05) weighed less, and levels of blood glucose (p < 0.05) and total cholesterol (p < 0.01) were reduced in PF compared with P.
Hun
C S
CS
Department of Biochemistry, Jichi Medical School, Tochigi-ken, 329-0498, Japan.
Hasegawa
K
K
Kawabata
T
T
Kato
M
M
Shimokawa
T
T
Kagawa
Y
Y
D000273:Adipose Tissue / Q000000981:diagnostic imaging / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D003920:Diabetes Mellitus / Q000378:metabolism; D004032:Diet; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D007473:Ion Channels; D020738:Leptin; D008297:Male; D026901:Membrane Transport Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D024101:Mitochondrial Proteins; D009765:Obesity; D009929:Organ Size; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D000071246:Uncoupling Protein 2; D017962:alpha-Linolenic Acid / Q000494:pharmacology
D001786:Blood Glucose; D015525:Fatty Acids, Omega-3; D007473:Ion Channels; D020738:Leptin; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D002784:Cholesterol; D015118:Eicosapentaenoic Acid