title: Isolation of highly purified autologous and allogeneic peripheral CD34+ cells using the CliniMACS device.
abstract: The CliniMACS CD34+ selection device was used for positive selection of apheresis products for autologous transplantation from 10 patients with malignant diseases and for allogeneic transplantation from 26 healthy donors. A total of 71 separations were performed. In 1 allogeneic donor, CD34+ progenitors were also isolated from bone marrow. Between 0.27 and 8.9 x 10(10) nucleated cells (median 4.9 x 10(10)) containing 0.09%-10.8% (median 0.67%) CD34+ progenitor cells were separated. After separation, a median number of 227 x 10(6) mononuclear cells (MNC) (51-524) were recovered, with a median viability of 99% (22%-100%) and a median purity of 97.0% (68.3%-99.7%) CD34+ cells. Depletion of T cells was extensive, with a median of 0.04% residual CD3+ cells (range <0.01%-0.92%). Residual CD19+ cells were between <0.01% and 17%, including CD34+CD19+ cells. Recovery of CD34+ cells was calculated according to the ISHAGE guidelines and ranged from 24% to 105% (median 71%). We conclude that with the CliniMACS device CD34+ cells with high purity and recovery can be isolated with concomitant effective T cell depletion in the allogeneic setting and with a high purging efficacy in the autologous setting.
authors.affiliation: Children's Hospital, University of Tuebingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schumm
authors.forename: P
authors.initials: P
authors.lastname: Lang
authors.forename: G
authors.initials: G
authors.lastname: Taylor
authors.forename: S
authors.initials: S
authors.lastname: Kuçi
authors.forename: T
authors.initials: T
authors.lastname: Klingebiel
authors.forename: H J
authors.initials: HJ
authors.lastname: Bühring
authors.forename: A
authors.initials: A
authors.lastname: Geiselhart
authors.forename: D
authors.initials: D
authors.lastname: Niethammer
authors.forename: R
authors.initials: R
authors.lastname: Handgretinger
mesh_terms: D018952:Antigens, CD34; D001772:Blood Cell Count; D001793:Blood Preservation; D019650:Hematopoietic Stem Cell Mobilization / Q000295:instrumentation / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D008212:Lymphocyte Depletion; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D018952:Antigens, CD34