Metabolism of L(-)-carnitine by Enterobacteriaceae under aerobic conditions.
Different Enterobacteriaceae, such as Escherichia coli, Proteus vulgaris and Proteus mirabilis, are able to convert L(-)-carnitine, via crotonobetaine, into gamma-butyrobetaine in the presence of carbon and nitrogen sources under aerobic conditions. Intermediates of L(-)-carnitine metabolism (crotonobetaine, gamma-butyrobetaine) could be detected by thin-layer chromatography. In parallel, L(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase activities were determined enzymatically. Monoclonal antibodies against purified CaiB and CaiA from E. coli O44K74 were used to screen cell-free extracts of different Enterobacteriaceae (E. coli ATCC 25922, P. vulgaris, P. mirabilis, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae) grown under aerobic conditions in the presence of L(-)-carnitine.
Elssner
T
T
Institut für Biochemie, Universität Leipzig, Germany.
Preusser
A
A
Wagner
U
U
Kleber
H P
HP
D000217:Acyltransferases; D000332:Aerobiosis; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002331:Carnitine / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000254:growth & development; D006836:Hydro-Lyases / Q000276:immunology / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000276:immunology / Q000378:metabolism*; D010088:Oxidoreductases / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; D010088:Oxidoreductases; C067153:crotonobetaine reductase; D000217:Acyltransferases; C060278:crotonobetainyl-CoA carnitine coenzyme A transferase; D006836:Hydro-Lyases; D002331:Carnitine