title: Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli.
abstract: To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solutions containing at least 0.25 g dry cell weight/L has been developed. The detection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metabolism, extraction of nucleotides, and quantitative analysis by ion-pair reversed-phase HPLC.
authors.affiliation: Institut für Biotechnologie, Universität Halle, Kurt-Mothes-Strasse 3, Halle, D-06120, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Meyer
authors.forename: N
authors.initials: N
authors.lastname: Noisommit-Rizzi
authors.forename: M
authors.initials: M
authors.lastname: Reuss
authors.forename: P
authors.initials: P
authors.lastname: Neubauer
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004734:Energy Metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006150:Guanine Nucleotides / Q000032:analysis* / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D006150:Guanine Nucleotides