Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enteritidis.
Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enterica serovar Enteritidis (S. enteritidis) is described. The procedure consists of plasmid DNA purification, its digestion with restriction endonuclease TaqI, electrophoresis, charge-coupled device camera scanning of the gels, and an analysis of the restriction patterns with the software Gel Manager. The system allowed us to analyse, in detail, results of plasmid profiling in more than 600 field strains of S. enteritidis. In addition to plasmid-free and virulence plasmid only containing strains, 15 additional plasmid types were detected. All the images and detailed protocols are available at the Web site http://www.clark.cz/vri/salmon.htm.
Rychlik
I
I
Veterinary Research Institute, Brno, Czech Republic. rychlik@vri.cz
Karpiskova
R
R
Faldynova
M
M
Sisak
F
F
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005516:Food Microbiology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D010957:Plasmids / Q000145:classification / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology; D012480:Salmonella Infections / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases