cpmA, a gene involved in an output pathway of the cyanobacterial circadian system.
We generated random mutations in Synechococcus sp. strain PCC 7942 to look for genes of output pathways in the cyanobacterial circadian system. A derivative of transposon Tn5 was introduced into the chromosomes of reporter strains in which cyanobacterial promoters drive the Vibrio harveyi luxAB genes and produce an oscillation of bioluminescence as a function of circadian gene expression. Among low-amplitude mutants, one mutant, tnp6, had an insertion in a 780-bp open reading frame. The tnp6 mutation produced an altered circadian phasing phenotype in the expression rhythms of psbAI::luxAB, psbAII::luxAB, and kaiA::luxAB but had no or little effect on those of psbAIII::luxAB, purF::luxAB, kaiB::luxAB, rpoD2::luxAB, ndhD::luxAB, and conII::luxAB. This suggests that the interrupted gene in tnp6, named cpmA (circadian phase modifier), is part of a circadian output pathway that regulates the expression rhythms of psbAI, psbAII, and kaiA.
Katayama
M
M
Department of Biology, Texas A&M University, College Station, Texas 77843-3258, USA.
Tsinoremas
N F
NF
Kondo
T
T
Golden
S S
SS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001683:Biological Clocks / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D056925:Circadian Rhythm Signaling Peptides and Proteins; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D045332:Photosystem II Protein Complex; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D056925:Circadian Rhythm Signaling Peptides and Proteins; D004251:DNA Transposable Elements; C114269:KaiA protein, cyanobacteria; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C119580:cpmA protein, Synechococcus sp.