title: Immunohistochemical detection of collagen type III and IV in relation with transformation of Ito cells in liver sinusoids of patients with reactive biliary hepatitis.
abstract: Reactive biliary hepatitis is a defined morphological entity, which is a result of chronic diseases of the gall bladder, biliary ducts or pancreas. The aim of the present study was to describe the morphology of reactive biliary hepatitis and its significance for progression of liver fibrosis, and in particular Ito cell (fat storing cell) transformation and occurrence of collagen type III and IV in the liver. Liver tissue from 19 patients with reactive biliary hepatitis was investigated light microscopically and immunohistochemically. Histologically, the liver showed features of mild to severe portal and lobular inflammation. The number of Ito cells increased periportally and pericentrally. Deposition of collagen type III and IV was increased in portal tracts, septa and perisinusoidal spaces, mainly in periportal zones of the lobules. Ultrastructurally, collagen type III immunoreactive fibrillar networks were found to be increased in the space of Disse around transitional cells. Collagen type IV immunoreactive deposits were detected around newly proliferating bile ducts in portal stroma and in the space of Disse. Ito cells were mainly transformed into transitional and myofibroblast-like cells. We discuss here the role of Ito cells and certain cytokines in the process of fibrosis of the liver in the course of reactive biliary hepatitis. It is proposed that bile acid retention in bile ducts during non-specific reactive inflammation or a gut endotoxin may cause transformation of Ito cells and increased collagen type III and IV in this type of hepatitis.
authors.affiliation: Department of General and Clinical Pathology, Faculty of Medicine Trakia University, Stara Zagora, Bulgaria.
authors.forename: M V
authors.initials: MV
authors.lastname: Gulubova
authors.forename: H D
authors.initials: HD
authors.lastname: Stoyanov
authors.forename: A E
authors.initials: AE
authors.lastname: Julianov
authors.forename: I V
authors.initials: IV
authors.lastname: Vasilev
authors.forename: I I
authors.initials: II
authors.lastname: Stoyanova
authors.forename: P B
authors.initials: PB
authors.lastname: Hadjipetkov
mesh_terms: D000328:Adult; D000368:Aged; D003094:Collagen / Q000032:analysis*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006521:Hepatitis, Chronic / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism
chemical_list: D003094:Collagen