title: A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency.
abstract: A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a "snorbozyme," is metabolically stable and cleaves a target U3 RNA with nearly 100% efficiency in vivo. This is the most efficient in vivo cleavage reported for a trans-acting ribozyme. A key advantage of the model substrate featured is that a stable, trimmed cleavage product accumulates. This property allows accurate kinetic measurements of authentic cleavage in vivo. The system offers new avenues for developing effective ribozymes for research and therapeutic applications.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Samarsky
authors.forename: G
authors.initials: G
authors.lastname: Ferbeyre
authors.forename: E
authors.initials: E
authors.lastname: Bertrand
authors.forename: R H
authors.initials: RH
authors.lastname: Singer
authors.forename: R
authors.initials: R
authors.lastname: Cedergren
authors.forename: M J
authors.initials: MJ
authors.lastname: Fournier
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D016337:RNA, Catalytic; D012342:RNA, Small Nuclear