Growth of HPV-18 immortalized human prostatic intraepithelial neoplasia cell lines. Influence of IL-10, follistatin, activin-A, and DHT.
Cultures from high grade prostatic intraepithelial neoplasia (HGPIN) have been established and immortalized by HPV-18 infection. The cultures were identified as PIN by Western blotting with anti-cytokeratin (34betaE12) and prostate specific antigen (PSA) antibodies. We examined the growth capabilities of the cultures in the presence of TGF-beta1, activin-A, follistatin (FS), androgens (DHEA, DHT) and several cytokines (IL-10, IL-2, IL-4). IL-10, FS, and DHT stimulated cell proliferation and colony forming ability, while the other cytokines and growth factors had no discernable effect. In addition, DHT and to a lesser extent IL-10 both stimulated PSA production. Activin-A blocked IL-10, FS, and DHT stimulated growth and PSA production. We interpret the data to mean that IL-10 induction of FS secretion (and FS binding of activin A) restores the normal growth capabilities of HGPIN cultures.
Wang
M
M
Department of Pathology and Laboratory Medicine, MCP-Hahnemann University, Philadelphia, PA 19102-1192, USA.
Liu
A
A
Garcia
F U
FU
Rhim
J S
JS
Stearns
M E
ME
D028341:Activins; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000728:Androgens / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003114:Colony-Forming Units Assay; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D038681:Follistatin; D006023:Glycoproteins / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007265:Inhibins / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D008027:Light; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008853:Microscopy; D027383:Papillomaviridae; D019048:Prostatic Intraepithelial Neoplasia / Q000473:pathology* / Q000821:virology; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000821:virology; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D000728:Androgens; D038681:Follistatin; D006023:Glycoproteins; D006133:Growth Substances; D013196:Dihydrotestosterone; D028341:Activins; D016753:Interleukin-10; D003687:Dehydroepiandrosterone; D007265:Inhibins