title: N-terminal domain, residues 1-91, of ribosomal protein TL5 from Thermus thermophilus binds specifically and strongly to the region of 5S rRNA containing loop E.
abstract: In this work we show for the first time that the overproduced N-terminal fragment (residues 1-91) of ribosomal protein TL5 binds specifically to 5S rRNA and that the region of this fragment containing residues 80-91 is a necessity for its RNA-binding activity. The fragment of Escherichia coli 5S rRNA protected by TL5 against RNase A hydrolysis was isolated and sequenced. This 39 nucleotides fragment contains loop E and helices IV and V of 5S rRNA. The isolated RNA fragment forms stable complexes with TL5 and its N-terminal domain. Crystals of TL5 in complex with the RNA fragment diffracting to 2.75 A resolution were obtained.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region. gongadze@vega.protres.ru
authors.forename: G M
authors.initials: GM
authors.lastname: Gongadze
authors.forename: V A
authors.initials: VA
authors.lastname: Meshcheryakov
authors.forename: A A
authors.initials: AA
authors.lastname: Serganov
authors.forename: N P
authors.initials: NP
authors.lastname: Fomenkova
authors.forename: E S
authors.initials: ES
authors.lastname: Mudrik
authors.forename: B H
authors.initials: BH
authors.lastname: Jonsson
authors.forename: A
authors.initials: A
authors.lastname: Liljas
authors.forename: S V
authors.initials: SV
authors.lastname: Nikonov
authors.forename: M B
authors.initials: MB
authors.lastname: Garber
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D016963:Thermus thermophilus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012341:RNA, Ribosomal, 5S; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; C106696:TL5 protein, Thermus thermophilus