Design and semisynthesis of spermine-sensitive Ribonuclease S'.
Spermine-sensitive stabilization of semisynthetic Ribonuclease S' was successfully carried out by sequence specific incorporation of a poly-anion domain into alpha-helix region of S-peptide.
Hamachi
I
I
Department of Chemistry & Biochemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka, Japan.
Yamada
Y
Y
Eboshi
R
R
Hiraoka
T
T
Shinkai
S
S
D000595:Amino Acid Sequence; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D012260:Ribonucleases / Q000138:chemical synthesis* / Q000737:chemistry*; D013096:Spermine / Q000737:chemistry*; D013696:Temperature
D013096:Spermine; D012260:Ribonucleases; C022542:ribonuclease S