[Angiotensin 1-converting enzyme gene insertion/deletion polymorphism in patients with coronary artery disease and essential hypertension and its nucleotide sequence].
OBJECTIVE
To determine angiotensin 1-coverting enzyme(ACE) gene insertion/deletion(I/D) polymorphism distributions in patients with coronary artery disease(CAD) and essential hypertension(EH),and to detect its nucleotide sequence.


METHODS
ACE genotypes of 137 patients with CDA, 42 patients with EH and 63 healthy people were detected by PCR methods. At the same time, nucleotide sequences of D and I allele were determined by fluorescein labeling automatic sequencing method.


RESULTS
the frequency of DD genotype in CAD group was significantly higher than that in control group (0.45 and 0.21,P < 0.01), but there was no significant difference of ACE geneI/D polymorphism distribution between EH and control group. The lengths of D and I alleles are 191bp and 479bp separately and their nucleotide sequences are somewhat different with the results of foreign countries.


CONCLUSIONS
ACE gene I/D polymorphism is an independent risk factor of CAD, but it has no relationship with EH. A 288bp insertion segment results in the I/D polymorphism of ACE gene.
Tan
H
H
Department of Cardiology, General Hospital of Jinan Army, Jinan, 250031, P. R. China.
Zhang
G
G
Huang
C
C
Zhu
K
K
Pan
Q
Q
D000328:Adult; D000368:Aged; D003327:Coronary Disease / Q000201:enzymology / Q000235:genetics*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic
D007703:Peptidyl-Dipeptidase A