title: Regulation of squid visual phospholipase C by activated G-protein alpha.
abstract: Phospholipase C (PLC) is the key enzyme in the phototransduction cascade of invertebrate rhabdomeric photoreceptors. In addition to 130 kDa PLC, a 95 kDa protein recognized by antibody against the catalytic site of PLC was found in the squid retina. The PLC-like 95 kDa protein (95 kDa PLC) was produced from 130 kDa PLC by an intrinsic protease in the presence of calcium. The 130 kDa PLC was stimulated by the active form of Gq-class G-protein alpha (Gq alpha), but the 95 kDa PLC was not, although their PLC activity was similar. A 35 kDa fragment, the counterpart of 95 kDa PLC, was not recognized by antibodies against catalytic site or N-terminal site of the 130 kDa PLC, indicating that the cleavage site is on the C-terminal side beyond the catalytic site. In the presence of a large excess of the 35 kDa fragment, 95 kDa PLC was stimulated by Gq alpha to a similar extent as intact 130 kDa PLC. These results indicate that the C-terminal polypeptide of PLC is necessary for regulation of its enzyme activity by Gq alpha. The uncoupling of PLC from Gq alpha, caused by limited proteolysis, is therefore a candidate regulatory mechanism of the phototransduction cascade in rhabdomeric photoreceptors.
authors.affiliation: Department of Pharmacology, Hyogo College of Medicine, Japan. suzutatu@hyo-med.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Narita
authors.forename: A
authors.initials: A
authors.lastname: Terakita
authors.forename: E
authors.initials: E
authors.lastname: Takai
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: Y
authors.initials: Y
authors.lastname: Kito
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukahara
mesh_terms: D000818:Animals; D001665:Binding Sites; D049832:Decapodiformes / Q000378:metabolism*; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D010738:Type C Phospholipases / Q000737:chemistry / Q000378:metabolism*; D014714:Vertebrates / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins