The choice of reference cell in the analysis of kinetic data using BIAcore.
An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the bulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data.
Ober
R J
RJ
Cancer Immunobiology Center, Center for Immunology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, Texas 75235-8576, USA.
Ward
E S
ES
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D002645:Chickens; D003627:Data Interpretation, Statistical; D007700:Kinetics; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D012015:Reference Standards; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000592:standards / Q000706:statistics & numerical data
D000911:Antibodies, Monoclonal; D009113:Muramidase