Biological properties of interferon-alpha produced Ex vivo by whole blood of patients infected by human immunodeficiency virus-1.
We investigated the biological properties of interferon (IFN)-alpha produced by Sendai virus (SV)-activated whole blood cultures in 20 patients infected with human immunodeficiency virus (HIV)-1 and 24 healthy controls. Supernatants of cultures were assayed for IFN-alpha by using an immunological method (DELFIA), biological methods and an in-vitro MxA induction assay. The levels of intracellular MxA protein were detected by an immunochemiluminescence assay. The levels of IFN-alpha in patients measured by DELFIA were significantly lower than those in healthy controls (P < 0.0001), but the antiviral activity of IFN-alpha in patients infected with HIV-1 was lower than predicted from DELFIA. The IFN-alpha produced by cells of patients infected with HIV-1 was able to induce MxA protein in human amnions WISH cells but was unable to protect these cells against Vesicular Stomatitis Virus (VSV)-induced cytopathic effects. A relative increased capability to induce the production of MxA protein in vitro was observed with the IFN-alpha contained in culture supernatant of virus-activated whole blood of HIV-1-infected patients with increased levels of MxA in their peripheral blood. These data suggest that biological properties of IFN-alpha produced in the course of HIV-1 infection are different from those observed with IFN-alpha of healthy subjects.
Chehadeh
W
W
Laboratoire de Virologie, Institut Gernez-Rieux, CHU, Lille, France.
Hober
D
D
Chieux
V
V
Alm
G
G
Harvey
J
J
Lion
G
G
Mouton
Y
Y
Wattré
P
P
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000276:immunology; D003588:Cytopathogenic Effect, Viral; D019204:GTP-Binding Proteins; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000097:blood; D064547:Myxovirus Resistance Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis
D016898:Interferon-alpha; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D019204:GTP-Binding Proteins