title: Dot immunobinding assay for detection of bovine herpesvirus 4 antibodies in rabbits.
abstract: A dot immunobinding assay (DIA) was used for the detection of antibody against bovine herpesvirus 4 (BHV-4) in experimentally infected specific-pathogen-free male and female rabbits. A semipurified virus preparation was used as the antigen, and protein A/G-horseradish-peroxidase conjugate and diaminobenzidine tetrahydrochloride were used as the detection system. Results of the DIA procedure on serum samples of experimentally infected male and female rabbits were compared with those of a complement-dependent virus neutralization (VN) test. None of the tested sera (0/60 samples) from either male or female rabbits were positive by the complement-dependent VN test. Results of the DIA procedure for the same tested sera were positive in 35 of 60 samples (58%) from BHV-4 infected rabbits, indicating higher sensitivity of DIA procedure as compared with the complement-dependent VN test.
authors.affiliation: Department of Pathology, Animal Reproduction Research Institute, El Haram, Giza, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Essmail
authors.forename: D
authors.initials: D
authors.lastname: Baker
authors.forename: J
authors.initials: J
authors.lastname: Collins
authors.forename: S
authors.initials: S
authors.lastname: Vandewoude
authors.forename: M
authors.initials: M
authors.lastname: Salman
authors.forename: A A
authors.initials: AA
authors.lastname: Hegazy
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D005260:Female; D015151:Immunoblotting / Q000379:methods*; D008297:Male; D009500:Neutralization Tests; D011817:Rabbits; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary; D018139:Simplexvirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral