title: Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections.
abstract: A commercial capture ELISA for specific IgM and IgG antibodies produced during dengue infection (PanBio Dengue Duo) showed excellent sensitivity (99%, n = 78) using sera collected at hospital discharge compared with established ELISA and hemagglutination inhibition (HAI) assays. Furthermore, the ELISA was able to diagnose 79% of the dengue cases using sera collected at hospital admission. The ELISA also showed high specificity (92%) in paired sera from patients without flavivirus infection (n = 26), although 45% of the patients with Japanese encephalitis (n = 20) showed elevation of IgG but not IgM. The IgG capture ELISA showed good correlation with the HAI assay (r = 0.83, P < 0.0001), and IgG levels could be used to distinguish between primary and secondary infection, with 100% of primary infections and 96% of secondary infections being correctly classified. This ELISA should prove useful in the clinical diagnosis of dengue infections.
authors.affiliation: United States Army Medical Component-Armed Forces Research Institute of Medical Science, Bangkok, Thailand.
authors.forename: D W
authors.initials: DW
authors.lastname: Vaughn
authors.forename: A
authors.initials: A
authors.lastname: Nisalak
authors.forename: T
authors.initials: T
authors.lastname: Solomon
authors.forename: S
authors.initials: S
authors.lastname: Kalayanarooj
authors.forename: M D
authors.initials: MD
authors.lastname: Nguyen
authors.forename: R
authors.initials: R
authors.lastname: Kneen
authors.forename: A
authors.initials: A
authors.lastname: Cuzzubbo
authors.forename: P L
authors.initials: PL
authors.lastname: Devine
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic