Antibodies against Helicobacter felis in sera of cats and dogs.
Serum samples from 61 dogs and 49 cats were screened for circulating antibodies against Helicobacter felis by an enzyme-linked immunosorbent assay (ELISA) using sonicated bacteria as an antigen. To improve the specificity of the ELISA, sera were absorbed with Campylobacter jejuni subsp. jejuni H. pylori as well as H. felis. Sera from 26 dogs (43%) and 19 cats (39%) revealed clear positive absorbance readings determined as an optical density (OD) that was statistically significant above the OD mean value [P < 0.025 (one-tailed); log10]. The absorbance pattern of ELISA-positive sera corresponded to results obtained with bovine and human reference sera. Furthermore, a correlation between the immune response and results from histopathological examination of gastric specimens from 22 dogs was demonstrated. It could be shown that antibodies against H. felis in sera of cats and dogs can easily be detected using an ELISA. The diagnostic value of this test must be evaluated in further investigations.
Seidel
K E
KE
Technische Universität München, Freising-Weihenstephan, Germany.
Stolte
M
M
Lehn
N
N
Bauer
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D016123:Campylobacter jejuni / Q000276:immunology / Q000302:isolation & purification; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D002415:Cats / Q000276:immunology*; D002417:Cattle; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000382:microbiology; D016998:Helicobacter / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D012016:Reference Values
D000907:Antibodies, Bacterial