title: Naturally occurring scrapie in Southdown sheep.
abstract: The purpose of this study was to characterize naturally occurring scrapie in the Southdown breed of sheep. Experimental subjects included 4 Southdown ewes admitted to the University of Missouri, College of Veterinary Medicine Large Animal Clinic. All 4 sheep had signs compatible with clinical scrapie. Cerebrospinal fluid (CSF) cell counts ranged from a low of 1 nucleated cell/microL to high of 4 cells/microL with a median of 3 cells/microL. Cerebrospinal protein concentrations ranged from 26 to 78 mg/dL with a median of 53 mg/dL. Immunoassay of the CSF for the 14-3-3 protein yielded positive results in 3 of the 4 sheep. Sequencing of the prion protein (PrP) gene revealed that all 4 sheep were homozygous for glutamine at codon 171 and, hence, were of the QQ genotype. Histopathologic examination of brain stem tissue sections revealed intracytoplasmic neuronal vacuolation and mild spongiform changes in the gray matter neuropil in all 4 ewes. The diagnosis of scrapie was confirmed by immunohistochemical staining for the abnormal PrP Our results suggest that the genetics of scrapie susceptibility are probably similar in Suffolk and Southdown sheep. Positive immunoassay results for the 14-3-3 protein were observed in 3 of the 4 sheep.
authors.affiliation: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA. tylerj@missouri.edu
authors.forename: J W
authors.initials: JW
authors.lastname: Tyler
authors.forename: D M
authors.initials: DM
authors.lastname: Weaver
authors.forename: J R
authors.initials: JR
authors.lastname: Turk
authors.forename: K I
authors.initials: KI
authors.lastname: O'Rourke
authors.forename: M G
authors.initials: MG
authors.lastname: Harrington
authors.forename: W
authors.initials: W
authors.lastname: Taylor
authors.forename: A
authors.initials: A
authors.lastname: Jenny
mesh_terms: D048948:14-3-3 Proteins; D000818:Animals; D001921:Brain / Q000473:pathology; D002555:Cerebrospinal Fluid / Q000166:cytology; D003062:Codon / Q000235:genetics; D004791:Enzyme Inhibitors / Q000032:analysis; D005260:Female; D020022:Genetic Predisposition to Disease / Q000662:veterinary*; D005973:Glutamine / Q000235:genetics*; D007150:Immunohistochemistry; D010375:Pedigree; D011328:Prions / Q000235:genetics*; D011506:Proteins / Q000032:analysis; D012608:Scrapie / Q000235:genetics*; D012756:Sheep; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D003062:Codon; D004791:Enzyme Inhibitors; D011328:Prions; D011506:Proteins; D005973:Glutamine; D014446:Tyrosine 3-Monooxygenase