title: Differences in plasma and abomasal kinetics of albendazole and its metabolites in calves grazed on pasture or fed a grain-based diet.
abstract: We evaluated the comparative plasma and abomasal fluid disposition kinetics of albendazole (ABZ) and its metabolites in calves either grazing on pasture or fed a grain-based concentrate diet. Six male Holstein calves (weight 180 to 200 kg) were allowed to graze on lush pasture for three weeks before intraruminal administration of ABZ at 10 mg kg-1(pasture group). After a three-week wash-out period, the same animals were housed and fed on a grain-based concentrate diet for three weeks prior to receiving the same ABZ treatment (concentrate group). Jugular blood and abomasal fluid samples were collected over 120 hours post-treatment. Plasma and abomasal fluid samples were analysed by high performance liquid chromatography (HPLC). The digesta transit time was measured using cobalt (Co) as a fluid marker; abomasal fluid and faecal samples were collected and Co concentrations measured by atomic absorption spectrophotometry. Complementary studies of the in vitro dissolution of ABZ particles at different pH values were also conducted. The pH of abomasal fluid collected from animals kept under both feeding conditions was registered. Increased concentrations of ABZ sulphoxide (ABZSO) and sulphone (ABZSO2) in plasma, resulting in significantly higher Cmax and area under the curve (AUC) values for both metabolites, were obtained in calves fed on the concentrate diet compared to those grazing on pasture. Enhanced abomasal fluid levels of ABZ and ABZSO were observed in concentrate-fed calves. The mean retention time of the digestive fluid marker in the gastrointestinal (GI) tract was significantly longer in the animals fed the grain-based diet. The in vitro dissolution of ABZ at a pH value equivalent to that obtained in the abomasum of the concentrate-fed calves (1.75) was significantly greater than that obtained at the pH registered in pasture-fed animals (2.00). The characterisation of the kinetic/metabolic behaviours and the resultant efficacy of antiparasitic drugs in animals reared under different management conditions may be relevant in increasing parasite control in livestock.
authors.affiliation: Departmento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro, Tandil, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Sánchez
authors.forename: L
authors.initials: L
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Pis
authors.forename: M
authors.initials: M
authors.lastname: Quiroga
authors.forename: C
authors.initials: C
authors.lastname: Lanusse
mesh_terms: D000018:Abomasum / Q000378:metabolism*; D015766:Albendazole / Q000097:blood / Q000493:pharmacokinetics*; D000821:Animal Feed; D000818:Animals; D000871:Anthelmintics / Q000097:blood / Q000493:pharmacokinetics*; D000977:Antiparasitic Agents / Q000097:blood / Q000493:pharmacokinetics*; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D005772:Gastrointestinal Transit; D006863:Hydrogen-Ion Concentration; D008297:Male
chemical_list: D000871:Anthelmintics; D000977:Antiparasitic Agents; D015766:Albendazole