title: Detection and quantification of HIV-1 in semen: identification of a subpopulation of men at high potential risk of viral sexual transmission.
abstract: BACKGROUND
  To assess HIV burden in both acellular and cellular fractions of semen in men with different levels of blood plasma HIV RNA by a cross-sectional study.
  
  
  METHODS
  Fifty-two HIV-1-seropositive men (21 receiving antiretroviral therapy) with CD4 cell counts ranging from 1 to 1170 x 10(6)/l.
  
  
  METHODS
  Semen was separated into seminal plasma and fractions enriched in motile spermatozoa or non-spermatozoal cells. HIV RNA was quantified by the HIV-Monitor technique (Roche) in blood plasma, seminal plasma and spermatozoa fractions. HIV DNA or infectious virions in cellular fractions were detected by either PCR or qualitative viral culture.
  
  
  RESULTS
  HIV RNA was detected in 86.5% of seminal plasma specimens and in 14.6% of spermatozoa fractions; HIV DNA was detected in 57.1% of non-spermatozoal cell fractions. HIV RNA levels in blood plasma and seminal plasma were correlated (r5 = 0.56, P < 0.0001, Spearman's rank test). A majority of men had lower levels in seminal plasma than in blood plasma: one-third had HIV-positive seminal cell fractions. However, 20 men (38.5%) with HIV RNA levels in seminal plasma (median: 4.65 log10 copies/ml) comparable to or higher than those in blood plasma had all HIV-positive non-spermatozoal cells or spermatozoa fractions with a high frequency of positive cultures.
  
  
  CONCLUSIONS
  A high frequency of men had detectable HIV in semen. We identified a subpopulation demonstrating high levels of HIV RNA in seminal plasma, comparable to or higher than those in blood plasma, frequently associated with a substantial viral shedding in seminal cells, raising the possibility of viral production within the genital tract and suggesting heterogeneity in the potential of HIV sexual transmission among infected men.
authors.affiliation: Laboratoire de Virologie, CHU Necker-Enfants Malades, Hôpital Cochin Port-Royal, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Tachet
authors.forename: E
authors.initials: E
authors.lastname: Dulioust
authors.forename: D
authors.initials: D
authors.lastname: Salmon
authors.forename: M
authors.initials: M
authors.lastname: De Almeida
authors.forename: S
authors.initials: S
authors.lastname: Rivalland
authors.forename: L
authors.initials: L
authors.lastname: Finkielsztejn
authors.forename: I
authors.initials: I
authors.lastname: Heard
authors.forename: P
authors.initials: P
authors.lastname: Jouannet
authors.forename: D
authors.initials: D
authors.lastname: Sicard
authors.forename: C
authors.initials: C
authors.lastname: Rouzioux
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000097:blood; D012307:Risk Factors; D012661:Semen / Q000821:virology*; D012725:Sexual Behavior; D013094:Spermatozoa / Q000821:virology
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral