title: Proteomics: theoretical and experimental considerations.
abstract: Cellular engineering relies on the ability to decipher the genetic basis of various phenotypes. Emerging technologies for analyzing the biological function of the information encoded in the genome of particular organisms and/or tissues focus on the monitoring of transcription (mRNA) and translation (protein) processes. Elementary theoretical considerations presented in this article strongly suggest that a combination of mRNA and protein expression patterns should be simultaneously considered to fully develop a conceptual understanding of the functional architecture of genomes and gene networks. We propose a framework of experimental and mathematical methods for acquiring and analyzing quantitative proteomic information and discuss recent developments in proteome analytical technology.
authors.affiliation: School of Chemical Engineering, Cornell University, Ithaca, New York 14853-5201, and Biosciences Division, Wayzata, Minnesota 55391-2397, USA.
authors.forename: V
authors.initials: V
authors.lastname: Hatzimanikatis
authors.forename: L H
authors.initials: LH
authors.lastname: Choe
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011506:Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins