Effect of a micronized purified flavonoid fraction on in vivo platelet functions in the rat.
The aim of this study was to investigate the effects of a micronized purified flavonoid fraction (MPFF) on in vivo rat platelet functions. Platelet aggregation and disaggregation were evaluated by a noninvasive, automated isotope monitoring system (AimsPlus). Indium-labeled platelets were injected into anesthetized rats and stimulated by adenosine diphosphate (ADP) (10 microg/kg, i.v.) or collagen (50 microg/kg, i.v.). Fibrinogen binding to ex vivo ADP-activated platelets was determined by flow cytometry. MPFF (100 mg/kg, p.o.) significantly reduced ADP-induced platelet aggregation (p<0.05) and increased platelet disaggregation (p<0.05) compared with controls. Moreover, MPFF inhibited collagen-induced platelet aggregation (p<0.001) and increased platelet disaggregation (p<0.01). In addition, fibrinogen binding to 2.5 or 5 microM ADP-stimulated platelets also was reduced significantly (p<0.05 and 0.01, respectively). These results show that MPFF inhibits in vivo rat platelet functions.
McGregor
L
L
INSERM U331, Faculty of Medicine RTH Läennec, Lyon, France. lmc@laennec.univ-lyon1.fr
Bellangeon
M
M
Chignier
E
E
Lerond
L
L
Rousselle
C
C
McGregor
J L
JL
D000818:Animals; D003094:Collagen / Q000494:pharmacology; D004145:Diosmin / Q000494:pharmacology*; D004338:Drug Combinations; D005419:Flavonoids / Q000494:pharmacology*; D006569:Hesperidin / Q000494:pharmacology*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D004338:Drug Combinations; D005419:Flavonoids; D010975:Platelet Aggregation Inhibitors; C080675:S 5682; D004145:Diosmin; D003094:Collagen; D006569:Hesperidin