title: Down-regulation of liver RNA breakdown by turpentine administration in the starved rat: autophagy and relevant factors.
abstract: OBJECTIVE
  To determine whether the inhibition of RNA breakdown observed in ad libitum fed rats 24 h after turpentine administration still occurs in inflamed rats fasted for 24 h and to examine the mechanism and factors involved.
  
  
  METHODS
  RNA breakdown was measured during cyclic in situ perfusion of livers by the accumulation of [14C] cytidine after in vivo RNA labelling. Autophagic activity was determined by the morphometric analysis of lysosomal structures.
  
  
  RESULTS
  The decrease in RNA breakdown (53%) observed in the inflamed rats was accompanied by a 38% drop in the fractional cytoplasmic volume of initial and digestive autophagic vacuoles. Among amino acids, only the portal levels of glutamate were significantly enhanced by 83%. In vivo suppression of glucocorticoid activity using RU 38486 in inflamed rats did not affect the inhibition of RNA breakdown.
  
  
  CONCLUSIONS
  The results show that turpentine-induced inflammation in fasted rats inhibits RNA degradation as well as autophagy and that glucocorticoids do not seem to be involved.
authors.affiliation: Laboratoire de Biologie Cellulaire, U 327 de l'Institut National de la Santé de la Recherche Médicale (INSERM), Faculté de Médecine Xavier Bichat, Université Paris 7, France.
authors.forename: A
authors.initials: A
authors.lastname: Saadane
authors.forename: D
authors.initials: D
authors.lastname: Delautier
authors.forename: V
authors.initials: V
authors.lastname: Lestriez
authors.forename: G
authors.initials: G
authors.lastname: Feldmann
authors.forename: B
authors.initials: B
authors.lastname: Lardeux
authors.forename: F
authors.initials: F
authors.lastname: Bleiberg-Daniel
mesh_terms: D000208:Acute Disease; D000818:Animals; D015536:Down-Regulation; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D010477:Perfusion; D012313:RNA / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors; D013217:Starvation; D014425:Turpentine / Q000494:pharmacology*
chemical_list: D011965:Receptors, Glucocorticoid; D015735:Mifepristone; D012313:RNA; D014425:Turpentine