Feasibility of an in vitro microbiological model as an alternative to the rabbit eye model.
Ocular inserts of gentamicin sulfate with polyvinyl alcohol (PVA) 1.5%, 2.0%, and 2.5% and a combination of methyl cellulose 2% and Eudragit NE 30D 30%, 35%, and 40% w/w of methyl cellulose were fabricated by a casting technique. The inserts were sterilized by gamma radiation at 25 kGy and tested for sterility. The microbiological efficacy of the ocular inserts against Staphylococcus aureus ATCC 6538P and Pseudomonas aeruginosa NCIM 2200 was evaluated by developing an in vitro microbiological model and an in vivo noninvasive rabbit eye model. Parameters of the in vitro microbiological model were varied, and the results correlated with a noninvasive rabbit eye model. The in vitro model proved to be a viable alternative to the rabbit eye model in evaluating the microbiological efficacy of gentamicin sulfate ocular inserts.
Devarajan
P V
PV
Department of Chemical Technology, University of Mumbai, Matunga, India.
Bhogte
C P
CP
Majali
A B
AB
Sabharwal
S
S
D000287:Administration, Topical; D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D005069:Evaluation Studies as Topic; D005123:Eye / Q000382:microbiology*; D005839:Gentamicins / Q000008:administration & dosage*; D066298:In Vitro Techniques; D008297:Male; D008954:Models, Biological; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors
D005839:Gentamicins; D011142:Polyvinyl Alcohol