title: Toward an in vitro test for the diagnosis of allergy to penicillins. Synthesis, characterization, and use of beta-lactam and beta-lactam metabolite poly-L-lysines which recognize human IgE antibodies.
abstract: Conjugates of poly-L-lysine (PLL) containing a penicillin or a penicilloyl residue were prepared and characterized by 1H NMR and by size-exclusion (SE) HPLC. These conjugates were used in a radio allergo sorbent tests (RAST) test for the determination of allergy toward beta-lactams. The chemiometric evaluation of the data indicates that allergy to amoxycillin is different from allergy to the other beta-lactams tested. Furthermore, careful chemical characterization of the conjugates appears to be crucial to obtain meaningful information from the RAST data.
authors.affiliation: Dipartimento di Sicenze dell'Ambiente e del Territorio, Universita' di Milano, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bolzacchini
authors.forename: V
authors.initials: V
authors.lastname: Consonni
authors.forename: P
authors.initials: P
authors.lastname: Gramatica
authors.forename: S
authors.initials: S
authors.lastname: Meinardi
authors.forename: M
authors.initials: M
authors.lastname: Orlandi
authors.forename: B
authors.initials: B
authors.lastname: Rindone
authors.forename: R
authors.initials: R
authors.lastname: Todeschini
authors.forename: M
authors.initials: M
authors.lastname: Blanca
authors.forename: C
authors.initials: C
authors.lastname: Mayorga
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003888:Desensitization, Immunologic; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D010406:Penicillins / Q000009:adverse effects / Q000276:immunology*; D011107:Polylysine / Q000737:chemistry / Q000276:immunology*; D011852:Radioallergosorbent Test; D047090:beta-Lactams / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*
chemical_list: D010406:Penicillins; D047090:beta-Lactams; D011107:Polylysine; D007073:Immunoglobulin E