Isolation of highly purified autologous and allogeneic peripheral CD34+ cells using the CliniMACS device.
The CliniMACS CD34+ selection device was used for positive selection of apheresis products for autologous transplantation from 10 patients with malignant diseases and for allogeneic transplantation from 26 healthy donors. A total of 71 separations were performed. In 1 allogeneic donor, CD34+ progenitors were also isolated from bone marrow. Between 0.27 and 8.9 x 10(10) nucleated cells (median 4.9 x 10(10)) containing 0.09%-10.8% (median 0.67%) CD34+ progenitor cells were separated. After separation, a median number of 227 x 10(6) mononuclear cells (MNC) (51-524) were recovered, with a median viability of 99% (22%-100%) and a median purity of 97.0% (68.3%-99.7%) CD34+ cells. Depletion of T cells was extensive, with a median of 0.04% residual CD3+ cells (range <0.01%-0.92%). Residual CD19+ cells were between <0.01% and 17%, including CD34+CD19+ cells. Recovery of CD34+ cells was calculated according to the ISHAGE guidelines and ranged from 24% to 105% (median 71%). We conclude that with the CliniMACS device CD34+ cells with high purity and recovery can be isolated with concomitant effective T cell depletion in the allogeneic setting and with a high purging efficacy in the autologous setting.
Schumm
M
M
Children's Hospital, University of Tuebingen, Germany.
Lang
P
P
Taylor
G
G
Kuçi
S
S
Klingebiel
T
T
Bühring
H J
HJ
Geiselhart
A
A
Niethammer
D
D
Handgretinger
R
R
D018952:Antigens, CD34; D001772:Blood Cell Count; D001793:Blood Preservation; D019650:Hematopoietic Stem Cell Mobilization / Q000295:instrumentation / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D008212:Lymphocyte Depletion; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D018952:Antigens, CD34