[The relation between mitochondrial DNA mutation and aminogly- coside antibiotics-induced deafness].
OBJECTIVE
To nvestigate the relation between mitochondrial DNA mutation and aminoglycoside antibiotics-induced deafness.


METHODS
Mitochondrial DNA (mtDNA) of peripheral blood from 48 sporadic cases was analysed using PCR-BsmAI digestion and PCR-direct DNA silver sequencing.


RESULTS
Six of 48 sporadic patients had A to G mutation in 12S rRNA gene of mtDNA at nucleotide 1555.


CONCLUSIONS
The results suggest that mtDNA 1555(G) mutation is related to the occurring of aminoglycoside antibiotics-induced deafness which can cause genetic susceptibility to aminoglycoside antibotics ototoxicity.
Zhang
L
L
Department of Biology, Nanjing Railway Medical College, Nanjing, 210009 P. R. China. LishanZh@jlonline.com
Lu
M
M
Huang
Y
Y
Zhou
X
X
Qiu
D
D
Wang
W
W
D000293:Adolescent; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D002648:Child; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000139:chemically induced*; D020022:Genetic Predisposition to Disease; D006801:Humans; D009154:Mutation
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial