title: Effect of transforming RNA on the synthesis of a protein with a secretory signal sequence in vitro.
abstract: U5 small nuclear RNA itself can act as a clastogenic and transforming agent when transfected into cells. In the previous work, the 3' half of the U5 small nuclear RNA first stem structure (designated RNA3S) was capable of driving normal cells into tumorigenic cells when expressed with a poly(A) tail (RNA3S+). This transformation critically depended upon the polypurine sequence GGAGAGGAA in RNA3S+. In this work, we first examined the pre-beta-lactamase and luciferase (model secretory and nonsecretory proteins) translation with the in vitro synthesized RNA3S in rabbit reticulocyte lysate. The capped RNA3S with a poly(A) tail suppressed the translation. In addition, the polypurine sequence played a crucial role in affecting the secretory protein synthesis, indicating a primary action of RNA3S+. Further studies revealed that the oligodeoxynucleotides, corresponding to the polypurine and its antisense sequences, directly contacted 28 S rRNA in ribosome and 7SL RNA in signal recognition particle, respectively, and differentially affected the nascent chain elongation of secretory protein synthesis. These results suggest that RNA3S+ blocks a physiological regulatory function played by signal recognition particle and the ribosome in the secretory protein synthesis and support the idea that the transformation might result from a repressed cellular activity.
authors.affiliation: Division of Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-0037, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Kumazaki
authors.forename: S
authors.initials: S
authors.lastname: Satoh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004792:Enzyme Precursors / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D011506:Proteins / Q000378:metabolism; D012339:RNA, Ribosomal, 28S / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics / Q000494:pharmacology*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D017421:Sequence Analysis; D018271:Signal Recognition Particle / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D004792:Enzyme Precursors; D021382:Protein Sorting Signals; D011506:Proteins; D012339:RNA, Ribosomal, 28S; D012342:RNA, Small Nuclear; D018271:Signal Recognition Particle; D011061:Poly A; D008156:Luciferases; D016914:Ribonuclease H; C037640:pre-beta-lactamase; D001618:beta-Lactamases