Gene therapy study of cytokine-transfected xenogeneic cells (Vero-interleukin-2) in patients with metastatic solid tumors.
On the basis of compelling preclinical data in cats and dogs, we initiated a clinical gene therapy study in nine patients with advanced solid tumors using xenogeneic fibroblasts secreting human interleukin (IL)-2 (Vero-IL-2 cells). Cohorts of three successive patients with tumors accessible to computed tomography- or ultrasound-guided injection were treated repeatedly with 5 x 10(5), 5 x 10(6), or 5 x 10(7) Vero-IL-2 cells. The endpoints of the study were feasibility, toxicity, and the clinical and biological effects of this novel approach to immunotherapy of cancer. Histopathological, immunological, and molecular analyses were performed on biopsy specimens of tumors and blood samples before, during, and after treatment. Treatment was well tolerated, and toxicity consisted of transient fever in one patient and short-lived, mild itching and erythema in two others. One patient with soft-tissue sarcoma showed a reduction of >90% and >50% of the volume of two distant, noninjected metastases, lasting for 29+ and 26 months, respectively. Four other patients showed stabilization of their disease for 3-9 months; of these patients, one with melanoma developed marked vitiligo. We conclude that repeated injections of < or =5 x 10(7) Vero-IL-2 cells are feasible and safe in heavily pretreated patients with advanced solid tumors. An additional evaluation of an intratumoral application of Vero-IL-2 seems warranted.
Rochlitz
C
C
Division of Medical Oncology, Kantonsspital, Basal, Switzerland. rochlitzc@ubaclu.unibas.ch
Jantscheff
P
P
Bongartz
G
G
Dietrich
P Y
PY
Quiquerez
A L
AL
Schatz
C
C
Mehtali
M
M
Courtney
M
M
Tartour
E
E
Dorval
T
T
Fridman
W H
WH
Herrmann
R
R
D000328:Adult; D000368:Aged; D000818:Animals; D001706:Biopsy; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000628:therapy; D017252:CD3 Complex / Q000235:genetics; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000235:genetics*; D005260:Female; D015316:Genetic Therapy / Q000009:adverse effects; D006801:Humans; D007150:Immunohistochemistry; D007376:Interleukin-2 / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D011859:Radiography; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014709:Vero Cells; D014820:Vitiligo / Q000139:chemically induced
D017252:CD3 Complex; D016207:Cytokines; D007376:Interleukin-2