Investigations on airborne microorganisms in animal stables. 2. Report: further characterization of airborne Clostridium perfringens.
Toxovars of 97 airborne C. perfringens isolates and 10 C. perfringens isolates from fecal samples of a calf stable were determined by an EIA procedure. Most airborne and fecal isolates belonged to toxovar A (88.7% and 80.0% respectively). Eight point two% of airborne C. perfringens were identified as toxovar C and 3.1% as toxovar D. Toxovar B was not found in the airborne state. Twenty% of fecal C. perfringens belonged to toxovar D. Toxovar B and C was not isolated from fecal samples. In addition, all fecal and air-borne isolates of C. perfringens toxovar D strains were analyzed in SDS-PAGE for their polypeptide pattern. All isolates from both sources exhibited the same polypeptide pattern after electrophoretic analysis in SDS-PAGE. Both results, determination of toxovars as well as polypeptide pattern analysis in SDS-PAGE, suggest that a major source of airborne C. perfringens in animal stables is animal feces.
Draz
A
A
Institute of Animal Hygiene and Environment Protection, Free University Berlin, Germany.
Chai
T
T
Zucker
B A
BA
D000391:Air Microbiology; D016902:Air Pollution, Indoor; D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification; D002417:Cattle; D003016:Clostridium perfringens / Q000145:classification / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D006799:Housing, Animal / Q000592:standards*; D010455:Peptides / Q000302:isolation & purification
D001426:Bacterial Proteins; D010455:Peptides