title: Assessment of the genotoxicity of contaminated soil with the Allium/Vicia-micronucleus and the Tradescantia-micronucleus assays.
abstract: The present study concerns the genotoxicity of contaminated soil near Metz, France. Three plant bioassays, the Vicia faba (broad bean), the Allium cepa (white onion) and the Tradescantia (spiderwort) micronuclei tests were used to evaluate for genotoxicity. Two soil samples were tested: soil sample A (from an industrial waste site) and soil sample B (from a cokeworks waste site). Maleic hydrazide was used as the positive control. Aqueous extracts of the soil samples were used to treat the roots of Vicia and Allium, and plant cuttings of Tradescantia according to the standard protocol for these plant assays established by the International Program on Chemical Safety and the World Health Organization. The results of these tests showed differential sensitivity in the three different bioassays. Soil sample A was more toxic than soil sample B.
authors.affiliation: Centre des Sciences de l'Environnement, Université de Metz, B.P. 4025, 57040, Metz Cedex 1, France.
authors.forename: S
authors.initials: S
authors.lastname: Cotelle
authors.forename: J F
authors.initials: JF
authors.lastname: Masfaraud
authors.forename: J F
authors.initials: JF
authors.lastname: Férard
mesh_terms: D000490:Allium / Q000187:drug effects / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D007887:Fabaceae / Q000187:drug effects / Q000235:genetics; D005602:France; D005843:Geography; D007220:Industrial Waste; D008670:Metals / Q000633:toxicity; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010944:Plants / Q000187:drug effects* / Q000235:genetics; D010946:Plants, Medicinal; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000633:toxicity*; D013045:Species Specificity
chemical_list: D007220:Industrial Waste; D008670:Metals; D009153:Mutagens; D012987:Soil; D012989:Soil Pollutants