title: The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of gram-negative bacteria.
authors.affiliation: University of South Alabama, Department of Microbiology and Immunology, Mobile 36688, USA.
authors.forename: M F
authors.initials: MF
authors.lastname: Alexeyev
mesh_terms: D001709:Biotechnology; D004274:DNA, Recombinant / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D016254:Mutagenesis, Insertional; D011815:R Factors / Q000235:genetics
chemical_list: D004274:DNA, Recombinant