title: Influence of serum-free culture conditions on steroid 5alpha-reductase mRNA expression in rat C6 glioma cells.
abstract: Immunocytochemical studies previously showed that serum deprivation resulted in the appearance of steroid 5alpha-reductase (5alpha-R) in the cytoplasm of rat C6 glioma cells. To determine whether this increase in cytoplasmic 5alpha-R was due to changes in 5alpha-R gene expression, the effect of serum deprivation on 5alpha-R mRNA expression was examined. No significant change in the mRNA levels was observed in cells grown in serum-free culture medium. Therefore, the appearance of 5alpha-R immunoreactivity in the cell cytoplasm observed under serum-free conditions is probably not due to changes in 5alpha-R gene expression.
authors.affiliation: Department of Pharmacology, Tokushima University School of Medicine, 3-18-15 Kuramoto, Tokushima 770, Japan. km@basic.med.tokushima-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuruo
authors.forename: K
authors.initials: K
authors.lastname: Ishimura
authors.forename: S
authors.initials: S
authors.lastname: Her
authors.forename: R A
authors.initials: RA
authors.lastname: Bell
authors.forename: D L
authors.initials: DL
authors.lastname: Wong
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics*; D000818:Animals; D016895:Culture Media, Serum-Free; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D005910:Glioma / Q000378:metabolism*; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016895:Culture Media, Serum-Free; D012333:RNA, Messenger; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase