Application of polymerase chain reaction for determination of toxins in Escherichia coli strains isolated from pigs with diarrhea.
The PCR method was used for the determination of LTI, STI, STII enterotoxins and Stx2e toxin genes in E. coli strains isolated from pigs with diarrhea. It was shown that most of the strains (77.3%) were enterotoxigenic, producing mainly LTI and STII (30.8%) or STI and STII (21.3%) toxins. It was found that 10.6% of isolates possessed the stx2e genes responsible for the elaboration of shiga toxin 2e.
Osek
J
J
Department of Microbiology, National Veterinary Research Institute, Puławy, Poland.
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods*; D022603:Shiga Toxins; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D022603:Shiga Toxins