title: Fast C18 solid-phase desalting/delipidation of the human serum apolipoproteins for matrix-assisted laser desorption ionization and electrospray ionization mass spectrometric analysis.
abstract: A new method for the delipidation of human serum lipoproteins involving the use of a reversed-phase C18 solid-phase extraction (SPE) cartridge is introduced for use with matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry. This method is compared with two other methods of lipoprotein delipidation. The SPE method of delipidation produces a higher and more reproducible protein yield than the conventional liquid-liquid methanol-diethyl ether delipidation technique. Furthermore, the SPE method implements a fast, sequential, desalting and delipidation of the lipoproteins for subsequent mass spectrometric analysis providing high quality spectra.
authors.affiliation: Texas A&M University, Department of Chemistry, College Station 77842-3012, USA.
authors.forename: L K
authors.initials: LK
authors.lastname: Watkins
authors.forename: P V
authors.initials: PV
authors.lastname: Bondarenko
authors.forename: D C
authors.initials: DC
authors.lastname: Barbacci
authors.forename: S
authors.initials: S
authors.lastname: Song
authors.forename: S L
authors.initials: SL
authors.lastname: Cockrill
authors.forename: D H
authors.initials: DH
authors.lastname: Russell
authors.forename: R D
authors.initials: RD
authors.lastname: Macfarlane
mesh_terms: D001053:Apolipoproteins / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D011804:Quinolines / Q000032:analysis; D012016:Reference Values; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014461:Ultracentrifugation
chemical_list: D001053:Apolipoproteins; D007202:Indicators and Reagents; D008055:Lipids; D008075:Lipoproteins, HDL; D011804:Quinolines; D012997:Solvents; C047117:bicinchoninic acid