Genetic mapping of the whirler mutation.
The whirler (wi) mutation on mouse Chromosome (Chr) 4 results in an autosomal recessive neuroepithelial deafness and vestibular dysfunction exhibited as a characteristic shaker-waltzer behavior (deafness, circling, and head-bobbing). We have constructed a genetic linkage map across the wi region in both an interspecific [(wi/wi x CAST/Ei)F1 x wi/wi] backcross (n = 817) and an intraspecific [(wi/wi x CBA/Ca)F1 x wi/wi)] backcross (n = 335). In the interspecific backcross, wi was found to be non-recombinant with Orm1, 0.12 cM distal of D4Mit87 and Ambp, and 0.12 cM proximal of CD301. In the intraspecific backcross, wi was found to be non-recombinant with Orm1 and D4Mit244, 0.3 cM distal of Mup1, and 0.6 cM proximal of Tnc. We also report a family from the interspecific backcross that shows evidence of multiple recombinations across the region of mouse Chr 4 around the wi locus. These rearrangements appear specific to both the region and the family.
Rogers
M J
MJ
MRC Institute of Hearing Research, University Park, Nottingham, UK.
Fleming
J
J
Kiernan
B W
BW
Mburu
P
P
Varela
A
A
Brown
S D
SD
Steel
K P
KP
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D051379:Mice; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains; D009154:Mutation; D013045:Species Specificity