Rapid high-performance liquid chromatographic determination of serum gabapentin.
Gabapentin (GBP) is a new antiepileptic drug approved for clinical treatment of partial seizures in the USA. Serum GBP concentrations in 283 patients were studied using high-performance liquid chromatography with fluorescence detection. The standard curves were linear over a range of 60 ng to 15 microg/ml. The coefficient of variations were 3.4 to 8.8% and 1.4 to 9.8% for intra- and inter-assay studies, respectively. The lower limit of quantitation was 10 ng/ml. Of the 283 patients studied, 72.5% had GBP levels between 2 and 10 microg/ml, 14.8% were below 2 microg/ml and 12.7% above 10 microg/ml. The mean+/-S.E. of GBP in 283 patients was 5.38+/-0.23 microg/ml. Peak concentrations of more than 15 microg/ml and trough levels as low as 0.1 microg/ml were not uncommon. The method described was rapid, simple, highly sensitive and reproducible. Other antiepileptic drugs and endogenous compounds did not interfere with the assay.
Jiang
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Specialty Laboratories, Santa Monica, CA 90404, USA.
Li
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D000085:Acetates / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000588:Amines; D000927:Anticonvulsants / Q000097:blood*; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids; D000077206:Gabapentin; D006801:Humans; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D005680:gamma-Aminobutyric Acid
D000085:Acetates; D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin