Early gene expression of NK cell-activating chemokines in mice resistant to Leishmania major.
Susceptibility of mice to Leishmania major is associated with an insufficient NK cell-mediated innate immune response. We analyzed the expression of NK cell-activating chemokines in vivo during the first days of infection in resistant and susceptible mice. The mRNA expression of gamma interferon-inducible protein 10 (IP-10), monocyte chemoattractant protein 1 (MCP-1), and lymphotactin was upregulated 1 day after infection in the draining lymph nodes of resistant C57BL/6 mice but not in those of susceptible BALB/c mice. In vivo local treatment of BALB/c mice with recombinant IP-10 shortly after infection resulted in an enhanced NK cell activity in the draining lymph node. The data suggest that although the recruitment of NK cells is normal in susceptible mice, the lack of NK cell-activating chemokines is a factor resulting in a suboptimal NK cell-mediated defense.
Vester
B
B
Institute for Clinical Microbiology and Immunology, University of Erlangen-Nürnberg, Erlangen, Germany.
Müller
K
K
Solbach
W
W
Laskay
T
T
D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics; D018946:Chemokine CCL5 / Q000235:genetics; D054357:Chemokine CXCL10; D018925:Chemokines / Q000235:genetics / Q000276:immunology*; D019744:Chemokines, C; D019743:Chemokines, CXC / Q000235:genetics / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D007113:Immunity, Innate / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008222:Lymphokines / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000235:genetics
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines; D019744:Chemokines, C; D019743:Chemokines, CXC; D008222:Lymphokines; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; C486249:Xcl1 protein, mouse; C018997:lymphotactin