title: Gallotannin hydrolysis by immobilized fungal mycelia in a packed bed bioreactor.
abstract: Hydrolysis of gallotannin to gallic acid by immobilized mycelia of Aspergillus niger MTCC 282, Aspergillus fischerii MTCC 150, Fusarium solani MTCC 350 and Trichoderma viride MTCC 167 in a packed bed bioreactor was studied. Fungal mycelia preinduced with 5 g L-1 gallotannin were immobilized in calcium alginate gel (1.5%) and the resultant beads were packed in a column to a bed volume of 175 mm3. Gallotannin dissolved in distilled water was passed through the column and the eluate was recycled after adjusting pH to 6 with ammonium hydroxide (10%). Maximum hydrolysis of gallotannin was recorded by immobilized mycelia of F. solani and T. viride at 35 degrees and 45 degrees C after 175 and 60 min of residency period respectively. Optimum substrate concentration required for maximum hydrolysis was 10 g L-1 at pH 5 for both the fungi. Immobilized mycelia of A. niger and A. fischerii revealed maximum operational stability. Loss of activity after eighth run was in the order of-A. niger (no loss), A. fischerii (7.5%), F. solani (18%) and T. viride (18%). Stability in terms of retention of enzyme activity after 150 days of storage at 4 degrees C was A. niger (58%), A. fischerii (26.8%), F. solani (83%) and T. viride (85.1%).
authors.affiliation: School of Life Sciences, Devi Ahilya University, Vigyan Bhawan, Indore, India.
authors.forename: B
authors.initials: B
authors.lastname: Bajpai
authors.forename: T
authors.initials: T
authors.lastname: Banerjee
authors.forename: S
authors.initials: S
authors.lastname: Patil
mesh_terms: D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D006868:Hydrolysis; D047348:Hydrolyzable Tannins / Q000378:metabolism*; D014242:Trichoderma / Q000254:growth & development / Q000378:metabolism*
chemical_list: D047348:Hydrolyzable Tannins