Isolation of highly enriched human monocytes from ten ml samples of heparinised whole blood.
A detailed study of the immune dysregulation involved in systemic inflammation requires the analysis of the main inflammatory cells in the circulation - the monocytes. Blood samples available from patients are necessarily restricted so that a rapid and efficient method is required to isolate these cells. Here we present a protocol to isolate blood monocytes in high yield from small samples of heparinised blood. The method yields monocyte preparations with a purity greater than 96%.
Wiegand
G
G
Institut für Immunologie und Transfusionsmedizin, Universität Greifswald, Germany.
Selleng
K
K
Gründling
M
M
Jack
R S
RS
D002469:Cell Separation; D005434:Flow Cytometry; D006493:Heparin; D006801:Humans; D018189:Immunomagnetic Separation; D009000:Monocytes / Q000166:cytology*
D006493:Heparin