Simple version of "megaprimer" PCR for site-directed mutagenesis.
Colosimo
A
A
Gene Therapy Core Center, University of California, San Francisco 94143, USA.
Xu
Z
Z
Novelli
G
G
Dallapiccola
B
B
Gruenert
D C
DC
D001483:Base Sequence; D001709:Biotechnology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D005069:Evaluation Studies as Topic; D006801:Humans; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*
C505032:CFTR protein, human; D017931:DNA Primers; D019005:Cystic Fibrosis Transmembrane Conductance Regulator