title: A gene (tmpA) for an efflux protein of the transporter family III from Brevibacterium linens OC2, an antibacterial substance-producing strain.
abstract: A gene (tmpA) encoding a putative transmembrane protein has been cloned from B. linens OC2, an antibacterial substance-producing strain. The deduced TmpA protein sequence shares similarities to members of the transporter family III exploiting the transmembrane proton gradient to provide export of toxic compounds such as antiseptics or antibiotics. Northern blot analysis indicated that tmpA gene is expressed. Length of RNA messenger and overlapping of ORFs upstream tmpA gene suggested that it might belong to an operon. The tmpA gene is unusual among B. linens species since it was not detected among eight B. linens collection strains and 40 B. linens industrial strains.
authors.affiliation: Institut de Génétique et Microbiologie, Unité Mixte de Recherche 2225, Centre National de la Recherche Scientifique, Bâtiment 360, Université Paris-Sud, 91405 Orsay Cedex 05, France.
authors.forename: C
authors.initials: C
authors.lastname: Boucabeille
authors.forename: J M
authors.initials: JM
authors.lastname: Simonet
authors.forename: G
authors.initials: G
authors.lastname: Henckes
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D015152:Blotting, Northern; D001951:Brevibacterium / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010455:Peptides
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010455:Peptides; C417482:TmpA protein, Brevibacterium linens