Expression of transforming growth factor-beta1, 2, 3 isoforms and type I and II receptors in acute focal cerebral ischemia: an immunohistochemical study in rat after transient and permanent occlusion of middle cerebral artery.
Transforming growth factor beta (TGF-beta) is involved in the modulation of cell growth, differentiation and repair following injury of various organs. Previous studies on human autopsy material have indicated that TGF-beta isoforms-beta1, -beta2 and -beta3, and TGF-beta receptor type I are expressed in various cells of necrotizing brain lesions like infarction and abscess. The present immunohistochemical study was designed to investigate changes that may occur with regard to TGF-beta and its receptors type I and II in a rat model of focal brain ischemia induced by transient or permanent occlusion of the middle cerebral artery. Our findings indicate that at days 1 and 3 following such transient and permanent ischemia there is an up-regulation of TGF-beta isoforms -beta1, -beta2 and -beta3, and TGF-beta receptor types I and II mainly in the perifocal neurons, reactive astroglial cells, endothelial cells and macrophages.
Ata
K A
KA
Department of Genetics and Pathology, University Hospital, Uppsala, Sweden. khaled_ahmad.ata@patologi.uu.se
Lennmyr
F
F
Funa
K
K
Olsson
Y
Y
Terént
A
A
D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D020768:Middle Cerebral Artery / Q000473:pathology*; D020033:Protein Isoforms; D051381:Rats; D017207:Rats, Sprague-Dawley; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis*; D016212:Transforming Growth Factor beta / Q000032:analysis*
D020033:Protein Isoforms; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta