title: Application of high-performance liquid chromatography of plasma fatty acids as their phenacyl esters to evaluate splanchnic and renal fatty acid balance in vivo.
abstract: Plasma fatty acids from renal and hepatic veins, and arterialized hand vein obtained in 20 subjects before and after insulin infusion were separated by reversed-phase high-performance liquid chromatography following phenacyl esterification. Separation and quantification over the range 1.0-100 nmol per injection of nine fatty acids was achieved within 60 min using [2H31]palmitic acid as internal standard. Analytical recoveries were greater than 90% and the intra- and inter-assay coefficients of variation were less than 2.5 and 4.0%, respectively. Following insulin infusion, net splanchnic uptake of total fatty acids decreased from 3.0+/-0.3 to 1.0+/-0.1 micromol/kg min (p<0.01), whereas net renal balance remained neutral (-0.04+/-0.04 vs. -0.06+/-0.03 micromol/kg min, p=N.S.). Individual fatty acid balance varied from a low of 0.012+/-0.005 (myristic acid) to a high of 0.95+/-0.08 (oleic acid) micromol/kg min across the splanchnic tissues and from 0.005+/-0.002 (stearic acid) to 0.21+/-0.1 (oleic acid) micromol/kg min across the kidney. There is a substantial diversity in changes in plasma concentration and regional balance of individual fatty acid during short-term fasting and hyperinsulinemia. This method is simple, accurate, and can be applied to assess individual fatty acid metabolism in vivo.
authors.affiliation: Department of Medicine, State University of New York at Stony Brook, 11794-8154, USA.
authors.forename: I
authors.initials: I
authors.lastname: Zaitseva
authors.forename: M
authors.initials: M
authors.lastname: Ajmal
authors.forename: E
authors.initials: E
authors.lastname: Cersosimo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters; D005069:Evaluation Studies as Topic; D005230:Fatty Acids, Nonesterified / Q000097:blood* / Q000737:chemistry; D006801:Humans; D007328:Insulin / Q000008:administration & dosage; D012015:Reference Standards; D012016:Reference Values; D012077:Renal Artery / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013152:Splanchnic Circulation
chemical_list: D004952:Esters; D005230:Fatty Acids, Nonesterified; D007328:Insulin