[The estimation of the amount of diphtheria toxin and its activity by various tests in the dynamic cultivation of Corynebacterium diphtheriae PW8].
Direct correlation between the results of tests for the biological activity of diphtheria toxin, carried out in vivo guinea pigs and in vitro in the microcytotoxicity test in CHO cell culture, has been established, which makes it possible to use the latter as one of the methods for the rapid, reproducible and economic evaluation of diphtheria toxin. The qualitative and quantitative evaluation of diphtheria toxin in the enzyme immunoassay with the use of monoclonal antibodies and in the microcytotoxicity test demonstrates that these two tests, when used for controlling cultivation processes, have essential advantages over the flocculation test as regards their specificity and information content.
Dmitrieva
M N
MN
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Gruber
I M
IM
Gavrilova
N A
NA
Titova
N G
NG
Iakovleva
I V
IV
Sviridov
V V
VV
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D002478:Cells, Cultured; D003353:Corynebacterium diphtheriae / Q000254:growth & development* / Q000276:immunology; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004167:Diphtheria Toxin / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D005260:Female; D010053:Ovary / Q000166:cytology / Q000187:drug effects; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D003470:Culture Media; D004167:Diphtheria Toxin