DNA flow cytometric quantification and DNA polymorphism analysis in the case of a complete mole with a coexisting fetus.
OBJECTIVE
Our purpose was to investigate whether DNA flow cytometric quantification and DNA polymorphism analysis are useful for cytogenetic diagnosis in the case of a complete hydatidiform mole that coexists with a living fetus.


METHODS
Flow cytometric analysis of the nuclear DNA content and polymerase chain reaction (PCR) amplification of the minisatellite locus with the MCT118 probe were performed on the tissues (fetus, placenta and mole) obtained at the initial evacuation.


RESULTS
DNA histograms of placental, fetal, and molar tissues showed diploid peaks. PCR products demonstrated that the allele of the mole was homozygous and inherited solely from the husband and that the mole differed genetically from the fetus and the placenta.


CONCLUSIONS
These results suggested that DNA flow cytometry and DNA polymorphism analysis may be useful for the cytogenetic diagnosis of a complete hydatidiform mole and a coexisting fetus.
Hirose
M
M
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Japan.
Kimura
T
T
Mitsuno
N
N
Wakuda
K
K
Takakura
K
K
Fujita
J
J
Noda
Y
Y
D000328:Adult; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000235:genetics; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics; D010920:Placenta / Q000000981:diagnostic imaging; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011272:Pregnancy, Multiple; D014427:Twins; D016216:Ultrasonography, Prenatal; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000235:genetics
D004273:DNA, Neoplasm; D004247:DNA