The interaction of mitogen-activated protein kinases to Epstein-Barr virus activation in Akata cells.
To understand the mechanism by which Epstein-Barr virus (EBV) is activated in Akata cells by cross-linking of surface immunoglobulin, the interaction between mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) and EBV activation was investigated. Immunoblotting using an anti-phosphoMAPK antibody (Ab) revealed that anti-IgG Ab induced rapid phosphorylation of MAPK in the cells. The phosphorylation was inhibited by MAPK/ERK kinase specific inhibitor, PD98059. The expressions of the EBV immediate early BZLF1 mRNA and its protein product ZEBRA, and early antigen were also inhibited by the inhibitor. These results indicate that MAPK is involved in the pathways of EBV activation.
Satoh
T
T
Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
Hoshikawa
Y
Y
Satoh
Y
Y
Kurata
T
T
Sairenji
T
T
D001402:B-Lymphocytes / Q000821:virology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000502:physiology*; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D010766:Phosphorylation; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism; D014764:Viral Proteins; D014775:Virus Activation
C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D005419:Flavonoids; D007074:Immunoglobulin G; D015534:Trans-Activators; D014764:Viral Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one