title: Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins.
abstract: We purified, cloned, and expressed aggrecanase, a protease that is thought to be responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4)] is a member of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373-alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.
authors.affiliation: Department of Inflammatory Diseases Research, DuPont Pharmaceuticals Company, Wilmington, DE 19880-0400, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Tortorella
authors.forename: T C
authors.initials: TC
authors.lastname: Burn
authors.forename: M A
authors.initials: MA
authors.lastname: Pratta
authors.forename: I
authors.initials: I
authors.lastname: Abbaszade
authors.forename: J M
authors.initials: JM
authors.lastname: Hollis
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.forename: S A
authors.initials: SA
authors.lastname: Rosenfeld
authors.forename: R A
authors.initials: RA
authors.lastname: Copeland
authors.forename: C P
authors.initials: CP
authors.lastname: Decicco
authors.forename: R
authors.initials: R
authors.lastname: Wynn
authors.forename: A
authors.initials: A
authors.lastname: Rockwell
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: J L
authors.initials: JL
authors.lastname: Duke
authors.forename: K
authors.initials: K
authors.lastname: Solomon
authors.forename: H
authors.initials: H
authors.lastname: George
authors.forename: R
authors.initials: R
authors.lastname: Bruckner
authors.forename: H
authors.initials: H
authors.lastname: Nagase
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: D M
authors.initials: DM
authors.lastname: Ellis
authors.forename: H
authors.initials: H
authors.lastname: Ross
authors.forename: B H
authors.initials: BH
authors.lastname: Wiswall
authors.forename: K
authors.initials: K
authors.lastname: Murphy
authors.forename: M C
authors.initials: MC
authors.lastname: Hillman
authors.forename: G F
authors.initials: GF
authors.lastname: Hollis
authors.forename: R C
authors.initials: RC
authors.lastname: Newton
authors.forename: R L
authors.initials: RL
authors.lastname: Magolda
authors.forename: J M
authors.initials: JM
authors.lastname: Trzaskos
authors.forename: E C
authors.initials: EC
authors.lastname: Arner
mesh_terms: D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; D000071121:ADAMTS4 Protein; D053674:Aggrecans; D000595:Amino Acid Sequence; D001168:Arthritis / Q000188:drug therapy; D002356:Cartilage / Q000378:metabolism; D020134:Catalytic Domain; D003001:Cloning, Molecular; D019483:Disintegrins / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D037181:Lectins, C-Type; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011348:Procollagen N-Endopeptidase; D011480:Protease Inhibitors / Q000494:pharmacology; D021382:Protein Sorting Signals; D011509:Proteoglycans / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017421:Sequence Analysis
chemical_list: D053674:Aggrecans; D019483:Disintegrins; D016326:Extracellular Matrix Proteins; D006877:Hydroxamic Acids; D007375:Interleukin-1; D037181:Lectins, C-Type; D011480:Protease Inhibitors; D021382:Protein Sorting Signals; D011509:Proteoglycans; D011994:Recombinant Proteins; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human; D008666:Metalloendopeptidases; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human