title: Effects of hypercholesterolaemia on Leydig and Sertoli cell secretory function and the overall sperm fertilizing capacity in the rabbit.
abstract: The effects of hypercholesterolaemia on testicular endocrine and exocrine function were evaluated. The influence of hypercholesterolaemia on sperm quality, quantity, and fertilizing potential was also determined. Ten mature rabbits (group A) were fed chow containing 3% cholesterol for 12 weeks. Ten control rabbits (group B) were fed normal chow for the same period. At the end of the experimental period testosterone profiles and sperm parameters were evaluated and the sperm reproductive potential was assessed by in vitro fertilization (IVF) techniques. Peripheral serum testosterone responses to testicular stimulation with human chorionic gonadotrophin, androgen-binding protein activity in testicular cytosols, sperm concentration, sperm motility, length of sperm midpiece, and IVF outcome were all significantly lower in group A than in group B. In contrast, serum cholesterol concentrations were significantly higher in group A. There were no significant differences in either testicular versus intra-abdominal temperature differences or cholesterol concentrations in seminal plasma or testicular tissue between groups A and B. The results suggest that hypercholesterolaemia has a detrimental effect on Leydig and Sertoli cell secretory function, spermatogenesis, epididymal sperm maturation process, and the overall sperm fertilizing capacity.
authors.affiliation: Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Shimamoto
authors.forename: N
authors.initials: N
authors.lastname: Sofikitis
authors.forename: I
authors.initials: I
authors.lastname: Miyagawa
mesh_terms: D000818:Animals; D001831:Body Temperature; D002784:Cholesterol / Q000032:analysis; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004822:Epididymis / Q000473:pathology; D005307:Fertilization in Vitro; D006801:Humans; D006937:Hypercholesterolemia / Q000209:etiology / Q000503:physiopathology*; D007985:Leydig Cells / Q000502:physiology*; D008297:Male; D009929:Organ Size; D011817:Rabbits; D012661:Semen / Q000737:chemistry; D012708:Sertoli Cells / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology*; D013739:Testosterone / Q000097:blood
chemical_list: D002791:Cholesterol, Dietary; D006063:Chorionic Gonadotropin; D013739:Testosterone; D002784:Cholesterol