Biosynthesis of polyketomycin produced by Streptomyces diastatochromogenes Tü 6028.
The biosynthesis of polyketomycin was investigated by feeding 13C-labeled acetate and propionate to the growing cultures of Streptomyces diastatochromogenes Tü 6028. 13C NMR spectral analysis demonstrated the polyketide origin of the aglycone and the dimethylsalicyloyl moieties. The O-methyl group and 6-CH3 of the aglycone as well as 3B-CH3 of L-axenose and 3C-CH3 of the salicyloyl residue were labeled by feeding L-[methyl-13C]methionine. Both deoxysugars emerged from D-glucose. The biosynthesis of the aglycone and the assembly of the glycoside are discussed. The polyketomycin producing strain may be a candidate for further exploration in combinatorial biosynthesis.
Paululat
T
T
Institut für Organische Chemie, Universität Göttingen, Germany.
Zeeck
A
A
Gutterer
J M
JM
Fiedler
H P
HP
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D005285:Fermentation; D006038:Glyoxylates / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013302:Streptomyces / Q000378:metabolism*; D014961:X-Ray Diffraction
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006038:Glyoxylates; C111268:polyketomycin