Preparation of a high titre Salmonella abortusovis H-antigen and antiserum and improvement of the microagglutination test.
Serological diagnosis of small ruminant abortions due to Salmonella abortusovis (S.a.o) was improved by using modified procedures to produce high titre H-antigens and anti-H serum. While the titres of the two standard antigens were 1:8 and 1:20, both modified antigens had a titre of 1:50. Similarly, the use of adjuvanted and non adjuvanted antigens for the production of anti-H hyperimmune serum resulted in a titre of 1:160,000 as compared to 1:20,000 when standard procedures were used. In addition, the slow micro-agglutination test has been modified and results can now be read within the same day.
Giannati
A
A
National Agricultural Research Foundation, Veterinary Institute of Infectious and Parasitic Diseases, Thessaloniki, Greece.
Koptopoulos
G
G
Hatzopoulou
E
E
Sarris
K
K
D000034:Abortion, Veterinary / Q000175:diagnosis* / Q000382:microbiology; D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D005260:Female; D011247:Pregnancy; D012475:Salmonella / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; C022863:H antigen