Separation and identification of corticosterone metabolites by liquid chromatography--electrospray ionization mass spectrometry.
High-performance liquid chromatography coupled to atmospheric pressure ionization-electrospray ionization mass spectrometry (API-ESI-MS) was investigated for the analysis of corticosterone metabolites; their characterization was obtained by combining the separation on Zorbax Eclipse XDB C18 column (eluted with a methanol-water-acetic acid gradient) with identification using positive ion mode API-ESI-MS and selected ion analysis. The applicability of this method was verified by monitoring the activity of steroid converting enzymes (20beta-hydroxysteroid dehydrogenase and 11beta-hydroxysteroid dehydrogenase) in avian intestines.
Miksík
I
I
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague. miksik@biomed.cas.cz
Vylitová
M
M
Pácha
J
J
Deyl
Z
Z
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000302:isolation & purification / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D003345:Corticosterone