title: Structural and functional studies on the overproduced L11 protein from Thermus thermophilus.
abstract: The L11 ribosomal protein from Thermus thermophilus (TthL11) has been overproduced and purified to homogeneity using a two-step purification protocol. The overproduced protein carries a similar methylation pattern at Lys-3 as does its homolog from Escherichia coli. Chymotrypsin digested only a small part of the TthL11 protein and did not cleave TthL11 into two peptides, as in the case of EcoL11, but produced only a single N-terminal peptide. Tryptic digestion of TthL11 also produced an N-terminal peptide, in contrast to the C-terminal peptide obtained with L11 from Bacillus stearothermophilus. The recombinant protein forms a specific complex with a 55-nt 23S rRNA fragment known to interact with members of the L11 family from several organisms. Cooperative binding of TthL11 and thiostrepton to 23S rRNA leads to an increased protection of TthL11 from tryptic digestion. The similar structural and biochemical properties as well as the significant homology between L11 from E. coli and B. stearothermophilus with the corresponding protein from Thermus thermophilus indicate an evolutionarily conserved protein important for ribosome function.
authors.affiliation: Laboratory of Biochemistry, School of Chemistry, Aristotle University of Thessaloniki, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Triantafillidou
authors.forename: M
authors.initials: M
authors.lastname: Simitsopoulou
authors.forename: F
authors.initials: F
authors.lastname: Franceschi
authors.forename: T
authors.initials: T
authors.lastname: Choli-Papadopoulou
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D008745:Methylation; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C027624:ribosomal protein L11