Characterization of the Arabidopsis lecRK-a genes: members of a superfamily encoding putative receptors with an extracellular domain homologous to legume lectins.
An Arabidopsis cDNA clone that defines a new class of plant serine/threonine receptor kinases was found to be a member of a family of four clustered genes (lecRK-a1-a4) which have been cloned, sequenced and mapped on chromosome 3. This family belongs to a large superfamily encoding putative receptors with an extracellular domain homologous to legume lectins and appears to be conserved at least among dicots. In the Columbia ecotype only the lecRK-a1 and perhaps the lecRK-a3 gene is functional, since lecRK-a2 is disrupted by a Ty-copia retroelement and lecRK-a4 contains a frameshift mutation. Structural analysis of the lecRK-al and lecRK-a3 deduced amino-acid sequences suggests that the lectin domain is unlikely to be involved in binding monosaccharides but could interact with complex glycans and/or with hydrophobic ligands. Immunodetection of lecRK gene products in plasma membranes purified by free-flow electrophoresis showed that the lecRK-a proteins are probably highly glycosylated integral plasma membrane components.
Hervé
C
C
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, UMR 215 CNRS-INRA, Castanet Tolosan, France.
Serres
J
J
Dabos
P
P
Canut
H
H
Barre
A
A
Rougé
P
P
Lescure
B
B
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001665:Binding Sites; D002874:Chromosome Mapping; D019143:Evolution, Molecular; D007887:Fabaceae / Q000235:genetics; D017343:Genes, Plant; D037102:Lectins / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D037121:Plant Lectins; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D011487:Protein Conformation; D011975:Receptors, Mitogen / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D011975:Receptors, Mitogen