title: Bacteriochlorin-protein interactions in native B800-B850, B800 deficient and B800-Bchla(p)-reconstituted complexes from Rhodopseudomonas acidophila, strain 10050.
abstract: Recently, a method which allows the selective release and removal of the 800 nm absorbing bacteriochlorophyll a (B800) molecules from the LH2 complex of Rhodopseudomonas acidophila strain 10050 has been described [Fraser, N.J. (1999) Ph.D. Thesis, University of Glasgow, UK]. This procedure also allows the reconstitution of empty binding sites with the native pigment Bchla(p), esterified with phytol. We have investigated the bacteriochlorophylla-protein interactions in native, B800 deficient (or B850) and in B8110-bacteriochlorophylla(p)-reconstituted LH2 complexes by resonance Raman spectroscopy. We present the first direct structural evidence which shows that the reconstituted pigments are correctly bound within their binding pockets.
authors.affiliation: Section de Biophysique des Protéines et des Membranes, DBCM/CEA and URA2096/CNRS, CEA-Saclay, Gif-sur-Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Gall
authors.forename: N J
authors.initials: NJ
authors.lastname: Fraser
authors.forename: M C
authors.initials: MC
authors.lastname: Bellissent-Funel
authors.forename: H
authors.initials: H
authors.lastname: Scheer
authors.forename: B
authors.initials: B
authors.lastname: Robert
authors.forename: R J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D012241:Rhodopseudomonas / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C023693:protein complex B800-850, Rhodobacter; D002338:Carotenoids