title: Contamination of intact apples after immersion in an aqueous environment containing Escherichia coli O157:H7.
abstract: The extent and location of Escherichia coli O157:H7 contamination after intact apples were immersed in cold (2 degrees C) 1% peptone water containing approximately 3x10(7) CFU/ml was assessed using four apple varieties, Golden Delicious, McIntosh, Red Delicious, and Braeburn. Room temperature and refrigerated apples were used to determine the effect of temperature differential on E. coli infiltration. The highest levels of E. coli were associated with the outer core region of the apple, followed by the skin. Apples were subsequently treated by immersing them for 1 min in 2,000 mg/liter sodium hypochlorite, followed by a 1-min tapwater rinse. This treatment reduced pathogen levels by 1- to 3-log cycles but did not eliminate the microorganism, particularly from the outer core region. While E. coli was not detected in the inner core of most apples, warm fruit immersed in cold peptone water occasionally internalized the pathogen. The frequency and extent of internalization of the pathogen was less when cold apples were immersed in cold peptone water. Subsequent dye uptake studies with Golden Delicious apples indicated that approximately 6% of warm apples immersed into a cold dye solution accumulated dye via open channels leading from the blossom end into the core region. However, dye uptake did not occur when the dye solution was warmer than the apple.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA. rbuchana@bangate.fda.gov
authors.forename: R L
authors.initials: RL
authors.lastname: Buchanan
authors.forename: S G
authors.initials: SG
authors.lastname: Edelson
authors.forename: R L
authors.initials: RL
authors.lastname: Miller
authors.forename: G M
authors.initials: GM
authors.lastname: Sapers
mesh_terms: D004396:Coloring Agents / Q000378:metabolism; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005511:Food Handling; D019685:Rosales / Q000382:microbiology*; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004396:Coloring Agents