Interaction of perindoprilat with human red blood cells.
The effects of perindoprilat on the morphology and dynamic properties of human erythrocytes were studied by light microscopy, electron spin resonance spectroscopy and spectrophotometric methods. Erythrocytes were exposed to perindoprilat at 37 degrees C for 30 and 120 min. It was shown that the drug at a concentration of 0.75 microg/ml did not cause significant changes in the structure of erythrocyte membranes. Higher doses of the drug (7.5 and 75 microg/ml) induced changes in membrane fluidity in the hydrophobic core of the lipid bilayer, the conformation of membrane proteins, the number of SH groups and the activity of membrane-bound acetylcholinesterase (AChE). These modifications were accompanied by changes in the shape of erythrocytes and did not depend on time of incubation. Therefore, it is proposed that perindoprilat perturbs the lipid bilayer and disturbs the organization of the protein-lipid environment.
Piasecka
A
A
Institute of Biophysics, University of Lódź, Poland.
Marciniak
K
K
Koter
M
M
Krajewska
E
E
Leyko
W
W
Bryszewska
M
M
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D048429:Cell Size / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000737:chemistry; D015394:Molecular Structure; D013113:Spin Labels; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D002800:Cholinesterase Inhibitors; D007211:Indoles; D008563:Membrane Lipids; D013113:Spin Labels; D013438:Sulfhydryl Compounds; C053500:perindoprilat