title: Identification of three distinct regions of allelic deletions on the short arm of chromosome 8 in hepatocellular carcinoma.
abstract: The chromosome 8p is associated with a large number of allelic imbalances in epithelial tumors including hepatocellular carcinoma (HCC). However, no tumor suppressor gene has been identified so far in this particular region of the genome. To further clarify the pattern of allelic deletions on chromosome 8p in HCC, we have undertaken high-density polymorphic marker analysis of 109 paired normal and primary tumor samples using 40 microsatellites positioned every 2 cm in average throughout 8p. We found that 60% of the tumors exhibited loss of heterozygosity (LOH) at one or more loci at 8p with three distinct minimal deleted areas: a 13 cm region in the distal part of 8p21, a 9 cm area in the more proximal portion of 8p22 and a 5 cm area in 8p23. These data strongly suggest the presence of at least three novel tumor suppressor loci on 8p in hepatocellular carcinoma.
authors.affiliation: Unité de Recombinaison & Expression Génétique, INSERM U163, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Pineau
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: S
authors.initials: S
authors.lastname: Prigent
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: G
authors.initials: G
authors.lastname: Gyapay
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
authors.forename: P
authors.initials: P
authors.lastname: Tiollais
authors.forename: M A
authors.initials: MA
authors.lastname: Buendia
authors.forename: A
authors.initials: A
authors.lastname: Dejean
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D002898:Chromosomes, Human, Pair 8; D017931:DNA Primers; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged
chemical_list: D017931:DNA Primers