title: Senescent erythrocytes: modification of rheologic properties, antigenic expression and interaction with monocytes.
abstract: Human erythrocytes have a well-defined lifespan of 120 days. Their eventual removal from circulation is a complex process affected by many cellular parameters, making them susceptible to sequestration in the spleen and other organs. The purpose of this study was to investigate putative changes in rheologic properties, antigenic expression and interaction with monocytes of senescent erythrocytes (SE). SE and young erythrocyte (YE) fractions were obtained by differential centrifugation from 20 healthy donor blood samples. Membrane rheomechanic properties (by diffractometric method), ABO and MN antigens reactivity and erythrophagocytosis by peripheral monocytes were investigated in each fractions. SE showed a little decrease in the deformability index and an increase of both membrane elastic modulus and surface viscosity. The studies performed indicate a decreased expression in the antigens of both blood group systems studied (p < 0.01) and an increased rate of erythrophagocytosis by monocytes in SE compared to YE (p < 0.01). The significant modifications in the biomechanic properties of senescent red blood cell membrane and the loss of antigenic expression could lead to physiological phagocytosis.
authors.affiliation: Laboratorio Inmunohematología, Hemorreología e Inmunogenética, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina. ccotorru@agatha.unr.edu.ar
authors.forename: A
authors.initials: A
authors.lastname: Racca
authors.forename: C
authors.initials: C
authors.lastname: Biondi
authors.forename: C
authors.initials: C
authors.lastname: Cotorruelo
authors.forename: S
authors.initials: S
authors.lastname: Galizzi
authors.forename: R J
authors.initials: RJ
authors.lastname: Rasia
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
authors.forename: J
authors.initials: J
authors.lastname: Valverde
mesh_terms: D000941:Antigens / Q000096:biosynthesis; D001809:Blood Viscosity; D002450:Cell Communication / Q000502:physiology*; D004905:Erythrocyte Aging / Q000276:immunology / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology; D012212:Rheology
chemical_list: D000941:Antigens