Isotope edited NMR studies of glycosidases: design and synthesis of a novel glycosidase inhibitor.
N-13C-methyl-deoxynojirimycin was synthesized and used in isotope-edited NMR studies to probe the binding site of an alpha-glucosidase. Results from this analysis led to the design and preparation of a novel alpha-glucosidase inhibitor, N-glycyl deoxynojirimycin.
Hines
J V
JV
Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Ohio State University, Columbus 43210, USA.
Chang
H
H
Gerdeman
M S
MS
Warn
D E
DE
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D005944:Glucosamine / Q000031:analogs & derivatives / Q000138:chemical synthesis; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000138:chemical synthesis* / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D005810:Multigene Family; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D017485:1-Deoxynojirimycin; D006026:Glycoside Hydrolases; D005944:Glucosamine; C013626:nojirimycin