Overexpression and amplification of c-myc in the Syrian hamster kidney during estrogen carcinogenesis: a probable critical role in neoplastic transformation.
An estrogen receptor-driven, multistep process for estrogen carcinogenesis in the Syrian hamster kidney is proposed. Because in this species the reproductive and urogenital tracts arise from the same embryonic germinal ridge, it is evident that the kidney has carried over genes that are responsive to estrogens. Using in situ hybridization, overexpression of early estrogen-response genes, i.e., c-myc and c-fos, has been shown to be localized preferentially in early renal tumor foci after 3.5-4.0 months of estrogen treatment. This event coincides with an increased number of S-phase proliferating cell nuclear antigen-labeled cells in these tumor foci, along with a rapid rise in aneuploid frequency in the kidney. Western blot analyses of c-MYC and c-FOS protein products support the overexpression of these genes. Amplification of c-myc, 2.4-3.6-fold, but not of c-fos, was detected in 67% of the primary renal tumors examined, by Southern blot analyses. Consistent chromosomal gains, common to both diethylstilbestrol- and estradiol-induced renal neoplasms, were observed in chromosomes 1, 2, 3, (6), 11, (13), 16, 20, and 21 (chromosome number alterations are indicated in parentheses). Using fluorescence in situ hybridization, the c-myc gene was localized to hamster chromosome 6qb. Chromosome 6 exhibited a high frequency of trisomies and tetrasomies in the kidney after 5.0 months of estrogen treatment and in primary renal tumors. The data presented indicate that estrogen-induced genomic instability may be a key element in carcinogenic processes induced by estrogens.
Li
J J
JJ
University of Kansas Cancer Institute, and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66160-7312, USA. jli1@kumc.edu
Hou
X
X
Banerjee
S K
SK
Liao
D Z
DZ
Maggouta
F
F
Norris
J S
JS
Li
S A
SA
D000782:Aneuploidy; D000818:Animals; D002292:Carcinoma, Renal Cell / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000235:genetics*; D002874:Chromosome Mapping; D003043:Cocarcinogenesis; D006224:Cricetinae; D004054:Diethylstilbestrol / Q000633:toxicity*; D004967:Estrogens; D005784:Gene Amplification; D005786:Gene Expression Regulation / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016762:Genes, fos; D016259:Genes, myc; D017403:In Situ Hybridization; D007621:Karyotyping; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008647:Mesocricetus / Q000235:genetics*; D009376:Neoplasms, Hormone-Dependent / Q000139:chemically induced / Q000235:genetics*; D009919:Orchiectomy; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000187:drug effects / Q000502:physiology*; D016196:S Phase; D013045:Species Specificity
D004967:Estrogens; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011960:Receptors, Estrogen; D004054:Diethylstilbestrol