Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum.
A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.
Lee
H S
HS
College of Pharmacy, Wonkwang University, Iksan, Korea. hslee@wonnms.wonkwang.ac.kr
Kim
Y S
YS
Kim
S B
SB
Choi
B E
BE
Woo
B H
BH
Lee
K C
KC
D006801:Humans; D007723:Korea; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D014784:Mistletoe / Q000737:chemistry*; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D014407:Tumor Cells, Cultured
D037102:Lectins; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum