title: Cytogenetic assignment of 53 microsatellites from the USDA-MARC porcine genetic map.
abstract: This study provides 53 new fluorescent in situ hybridization cytogenetic assignments for microsatellite markers linked on the swine genetic map. Forty microsatellites are physically assigned for the first time. The chromosomal locations of eight markers were either confirmed or refined, while five loci were assigned to locations different from those given in previous reports. Markers were selected to provide physical anchors based on their presumed proximity to centromeres or telomeres and at approximately 30 cM intervals across the genetic map. The number of physical anchors for pig (SSC) chromosomes 8, 15, and 18 linkage groups was significantly improved. Centromeric regions were localized to areas less than 10 cM for SSC 1, 2, 3, 6, 7, 8, and 9. Although the recombination rate was generally higher across small biarmed chromosomes and lowest for large acrocentric chromosomes, two regions with particularly low (1q2.1-->q2.9 and 13q2.3-->q4.1) and three regions with extremely high (5p1.5-->p1.2, 6p1.4-->p1.3, and 12p1.5-->p1.4) rates of recombination were detected. These assignments represent an overall 10% increase in the number of physically assigned markers in Sus scrofa and more than a 20% increase in the number of Type II loci assigned to the pig cytogenetic map.
authors.affiliation: US Meat Animal Research Center (MARC), Agricultural Research Service, US Department of Agriculture, Clay Center, NE 68933-0166, USA.
authors.forename: N L
authors.initials: NL
authors.lastname: Lopez-Corrales
authors.forename: C W
authors.initials: CW
authors.lastname: Beattie
authors.forename: G A
authors.initials: GA
authors.lastname: Rohrer
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003582:Cytogenetics; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D020161:Physical Chromosome Mapping; D013552:Swine / Q000235:genetics*