Changes in substance P and calcitonin gene-related peptide binding in the dorsal horn of rat spinal cord following pronase-induced deafferentation.
Quantitative receptor autoradiography was used to evaluate potential alterations in substance P (SP) and calcitonin gene-related peptide (CGRP) binding in the L4 spinal segment of rats following unilateral poisoning of the sciatic nerve with pronase. Ten days after pronase-induced deafferentation there was a significant increase in SP and CGRP binding in the superficial (I-II) and deeper (II-IV) laminae of the dorsal horn ipsilaterally. Densitometric measurements revealed a 50% return towards normal values for SP binding by 90 days postpronase injection in all laminae examined, while the density of CGRP binding showed a partial return towards normal values for laminae III-IV only. These differential responses may be indicative of the mechanisms underlying pronase-induced peripheral neuropathy.
Helgren
M E
ME
Physical Therapy Department, Quinnipiac College, Hamden, CT 06518, USA.
Wolfe
M
M
Arsenault
K
K
Kapadia
S E
SE
LaMotte
C C
CC
D000818:Animals; D001345:Autoradiography; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D007457:Iodine Radioisotopes; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism; D009437:Neuralgia / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D009475:Neurons, Afferent / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011402:Pronase; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000032:analysis / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000032:analysis / Q000378:metabolism; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism* / Q000494:pharmacology
D007457:Iodine Radioisotopes; D018015:Receptors, Calcitonin Gene-Related Peptide; D018040:Receptors, Neurokinin-1; D013373:Substance P; D011402:Pronase; D015740:Calcitonin Gene-Related Peptide