title: Viability studies on actinomycetes.
abstract: Eighty-nine Actinomycetes strains were tested for their viability, morphological and physiological characteristics after being kept under paraffin oil overlay and distilled water for a period between 10-30 years. Most of the studied strains belong to the "Lorenzo De Montemayor" collection. Almost all the recovered strains were 28-30 years old and had never been subcultured since the paraffin oil was overlaid. 71.4% of viable Streptomycetes strains had been kept on Sabouraud-dextrose agar and 28.6% were kept on Negroni and Bonfiglioli-medium. Streptomyces violaceusruber produced its characteristic pigment even after 28 years under these conditions. All of the recovered strains were tested for their biological activity, but only Streptomyces lavendulae showed growth-inhibition against Staphylococcus aureus and Bacillus subtilis.
authors.affiliation: Departamento de Química, Universidad Simón Bolívar, Caracas, Venezuela. ataddei@usb.ve
authors.forename: A
authors.initials: A
authors.lastname: Taddei
authors.forename: M M
authors.initials: MM
authors.lastname: Tremarias
authors.forename: C
authors.initials: C
authors.lastname: Hartung de Capriles
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D000193:Actinomycetales Infections / Q000382:microbiology; D018070:Biological Specimen Banks; D003470:Culture Media; D006801:Humans; D008828:Microbiological Techniques; D010232:Paraffin; D013048:Specimen Handling / Q000379:methods*; D013302:Streptomyces / Q000378:metabolism / Q000502:physiology; D013997:Time Factors; D014687:Venezuela
chemical_list: D003470:Culture Media; D010232:Paraffin