title: CD95 ligand is expressed in Reed-Sternberg cells of Hodgkin's disease.
abstract: Reed-Sternberg (RS) cells and their mononuclear variants, Hodgkin's (H) cells, are considered to be the neoplastic cells of Hodgkin's disease (HD). The cellular origin of H-RS cells remains the subject of considerable controversy, although most recent papers have claimed that H-RS cells are of B cell origin. Recently, however, it has been reported that some H-RS cells express granzyme B, as observed in cytotoxic T cells and/or natural killer cells, which also express CD95 ligand (FasL/APO-1L). In the present study, the expression of CD95L and granzyme B in H-RS cells of HD was investigated. CD95L was detected in H-RS cells in five of nine HD cases (one case of lymphocyte-rich classical HD, two of these cases of nodular sclerosis type, and two of four cases of mixed cellularity type). All three examined HD cell lines expressed CD95L in the cytoplasm, although cell surface expression was seen only in L428 cells. Three HD cases expressed both CD95L and granzyme B. It was concluded that CD95L is frequently expressed in H-RS cells, which is one of their notable characteristics; albeit it seems to be irrespective of cell lineage.
authors.affiliation: Department of Pathology, Okayama University Medical School, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Sakuma
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: K
authors.initials: K
authors.lastname: Omonishi
authors.forename: R
authors.initials: R
authors.lastname: Nishiuchi
authors.forename: N
authors.initials: N
authors.lastname: Teramoto
authors.forename: H
authors.initials: H
authors.lastname: Yanai
authors.forename: K
authors.initials: K
authors.lastname: Kawahara
authors.forename: I
authors.initials: I
authors.lastname: Kubonishi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuo
authors.forename: T
authors.initials: T
authors.lastname: Akagi
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D053804:Granzymes; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008024:Ligands; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D016539:Reed-Sternberg Cells / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D008024:Ligands; D012333:RNA, Messenger; D019014:fas Receptor; C509213:GZMB protein, human; D053804:Granzymes; C509214:Gzmb protein, mouse; D012697:Serine Endopeptidases