title: Comparative genomic hybridization defines frequent loss on 16p in human anaplastic thyroid carcinoma.
abstract: Anaplastic thyroid cancer (ATC) is one of the malignant tumors with the poor prognosis that is thought to arise from well-differentiated thyroid cancer (DTC). To investigate the molecular mechanism of ATC, we studied genomic alterations of eight ATC cell lines and three DTC cell lines by means of the comparative genomic hybridization (CGH) method. Loss of 16p was observed in five of eight ATC cell lines (62. 5%), but none of the three DTC cell lines showed loss of this chromosome arm. It is notable that loss of 18q [7/8 of ATC (87.5%), 2/3 of DTC (67%)] and gain of 20q [5/8 of ATC (62.5%), 3/3 of DTC (100%)] were frequently seen in both histologic types. Our results suggest that there is a gene in 16p that is closely associated with transformation from well-differentiated thyroid cancer to anaplastic cancer.
authors.affiliation: Department of Surgery II, Osaka University Medical School, Suita, Osaka 565-0871, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Komoike
authors.forename: Y
authors.initials: Y
authors.lastname: Tamaki
authors.forename: I
authors.initials: I
authors.lastname: Sakita
authors.forename: N
authors.initials: N
authors.lastname: Tomita
authors.forename: M
authors.initials: M
authors.lastname: Ohue
authors.forename: M
authors.initials: M
authors.lastname: Sekimoto
authors.forename: M
authors.initials: M
authors.lastname: Miyazaki
authors.forename: M
authors.initials: M
authors.lastname: Kadota
authors.forename: N
authors.initials: N
authors.lastname: Masuda
authors.forename: M
authors.initials: M
authors.lastname: Ooka
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Kozaki
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: N
authors.initials: N
authors.lastname: Matsuura
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: A
authors.initials: A
authors.lastname: Horii
authors.forename: M
authors.initials: M
authors.lastname: Monden
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002885:Chromosomes, Human, Pair 16; D017353:Gene Deletion; D006801:Humans; D019656:Loss of Heterozygosity; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured