Immunocytochemical study of parafollicular (C) cells of the thyroid in some wild rodents.
Studies were done on 3 wild species of rodents: field voles (Microtus agrestis, Linnaeus 1761), bank voles (Clethrionomys glareolus, Schreber 1780), and forest mice (Apodemus flavicollis, Melchior 1834). Immunocytochemical reactions were used to detect calcitonin (CT), calcitonin gene-related peptide (CGRP), neuron-specific enolase (NSE), chromogranin A (CgA) in the thyroid parafollicular (C) cells in all species examined. Antisera to human CT, rat CGRP, bovine CgA, rat NSE and human NSE give probably a positive reaction in all C cells in the rodents examined. However, in the NSE determining reaction, much feebler positive C cells were observed. Individual variation in respect of shape and distribution of C cells was observed in all species. In forest mice the C cells resembled in shape the C cells previously described in mouse and rat. In field voles and bank voles the C cells were assembled into small groups more often than in forest mice. Antibodies (anti-human CT, anti-rat CGRP, anti-bovine CgA, anti-rat NSE and anti-human NSE) used by us were good markers of C cells in the thyroids of the 3 species of free-living rodents examined.
Sawicki
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Department of Histology and Embryology, Academy of Medicine, Białystok, Poland.
Zabel
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D000818:Animals; D003411:Arvicolinae / Q000033:anatomy & histology*; D002116:Calcitonin / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry / Q000379:methods; D009115:Muridae / Q000033:anatomy & histology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats; D013045:Species Specificity; D013961:Thyroid Gland / Q000166:cytology*
D053379:Chromogranin A; D002864:Chromogranins; D007106:Immune Sera; D002116:Calcitonin; D010751:Phosphopyruvate Hydratase; D015740:Calcitonin Gene-Related Peptide