title: Gene delivery systems using the Sendai virus.
abstract: Fusogenic liposome (FL) is a delivery system that can transfer encapsulated materials into living cells directly through membrane fusion. FL is a promising approach for gene therapy because it can deliver various genetic materials much more efficiently than other non-viral vectors without damaging the cell. FL-mediated gene transfer consists of two independent membrane fusion phenomena; generation of a FL by fusing a Sendai virus (SV) particle with a simple liposome encapsulating DNA, and successive fusion of the FL with cell membrane. The former requires viral F protein but no other special molecule on the liposomal membrane, whereas the latter may require the receptor (sialic acid) and unidentified assistant molecule(s) on the cell membrane. Further analysis suggests that these assistant molecule(s), not the receptor, may control the fusion and govern the cell specificity of FL-mediated delivery. This review has described a detailed analysis of these fusion phenomena and discussed possible applications of FL-mediated gene delivery to human gene therapy.
authors.affiliation: Department of Neurovirology, Osaka University, Japan. mahito@biken.osaka-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Nakanishi
authors.forename: H
authors.initials: H
authors.lastname: Mizuguchi
authors.forename: K
authors.initials: K
authors.lastname: Ashihara
authors.forename: T
authors.initials: T
authors.lastname: Senda
authors.forename: A
authors.initials: A
authors.lastname: Eguchi
authors.forename: A
authors.initials: A
authors.lastname: Watabe
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.forename: M
authors.initials: M
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: A
authors.initials: A
authors.lastname: Masago
authors.forename: J
authors.initials: J
authors.lastname: Okabe
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Mayumi
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes / Q000648:ultrastructure; D008561:Membrane Fusion; D008954:Models, Biological; D018108:Respirovirus / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D008081:Liposomes