Characterization of the meta-cleavage compound hydrolase gene involved in degradation of the lignin-related biphenyl structure by Sphingomonas paucimobilis SYK-6.
Sphingomonas paucimobilis SYK-6 has the ability to transform a lignin-related biphenyl compound, 2,2'-dihydroxy-3,3'-dimethoxy-5, 5'-dicarboxybiphenyl (DDVA), to 5-carboxyvanillic acid (5CVA) via 2, 2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (OH-DDVA). In the 4.9-kb HindIII fragment containing the OH-DDVA meta-cleavage dioxygenase gene (ligZ), we found a novel hydrolase gene (ligY) responsible for the conversion of the meta-cleavage compound of OH-DDVA to 5CVA. Incorporation of 18O from H218O into 5CVA indicated there was a hydrolytic conversion of the OH-DDVA meta-cleavage compound to 5CVA. LigY exhibited hydrolase activity only toward the meta-cleavage compound of OH-DDVA, suggesting its restricted substrate specificity.
Peng
X
X
Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
Masai
E
E
Katayama
Y
Y
Fukuda
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001713:Biphenyl Compounds / Q000737:chemistry* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D006867:Hydrolases; D008031:Lignin; D010105:Oxygenases; C010574:biphenyl; C113517:2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl oxygenase; C120989:LigY hydrolase, bacteria