Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss.
Two rainbow trout chemokine receptors have been sequenced, with homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a mature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7. Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors. Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.
Daniels
G D
GD
Department of Zoology, University of Aberdeen, Scotland, United Kingdom.
Zou
J
J
Charlemagne
J
J
Partula
S
S
Cunningham
C
C
Secombes
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000276:immunology*; D009928:Organ Specificity / Q000276:immunology; D010802:Phylogeny; D051381:Rats; D054400:Receptors, CCR7; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000302:isolation & purification; D019707:Receptors, Chemokine / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
C515370:CCR7 protein, human; C515371:Ccr7 protein, mouse; C515372:Ccr7 protein, rat; D054400:Receptors, CCR7; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine