Setaria digitata in cattle of Thailand identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis.
Adult Thai Setaria worms collected from cattle which were bred, housed and slaughtered in Thailand were morphologically identified as Setaria digitata. Furthermore, in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) adult Thai S. digitata had the same protein profiles as adult Japanese S. digitata, but did not possess the protein with a molecular size of 69 kDa which was confirmed in adult S. marshalli. In addition, there were no differences in the protein profiles between male and female S. digitata. In point of the distribution pattern of the proteins ranging from 73 to 64 kDa revealed by 2D-PAGE, there were no differences between Thai and Japanese S. digitata, and between male and female worms of the species.
Subhachalat
P
P
Animal Health Laboratory, School of Agriculture, Ibaraki University, Japan.
Shirasaka
S
S
Nakajima
H
H
Adachi
Y
Y
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary*; D005260:Female; D008297:Male; D008970:Molecular Weight; D017182:Setaria Nematode / Q000302:isolation & purification*; D012719:Setariasis / Q000175:diagnosis / Q000453:epidemiology*; D013785:Thailand