title: On the structure of endogenous ouabain.
abstract: The ouabain-like sodium pump inhibitor in mammals (so-called "endogenous ouabain") has been considered a subtle structural isomer of ouabain. Its structural investigation, however, has long been hindered by the paucity of sample material. Our recent purification of endogenous ouabain (3 micrograms) from bovine hypothalamus allowed the measurement of its 1H-NMR. The obtained spectrum as well as reexamination of past microscale structural studies on endogenous ouabain led us to identify the purified material as ouabain in an unusual manner. It turned out that the structural analysis had been complicated by a facile ouabain-borate complexation in borosilicate glassware. In retrospect, it is not surprising that the polyhydroxylated ouabain molecule serves as a polydentate ligand to inorganic species. In its physiological environment, ouabain may exist as some unknown complex. The chemical species giving rise to the reported biological activities of hypothalamic inhibitory factor preparations remain to be clarified.
authors.affiliation: Department of Chemistry, Columbia University, New York, NY 10027, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kawamura
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Itagaki
authors.forename: C
authors.initials: C
authors.lastname: Bell
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: G T
authors.initials: GT
authors.lastname: Haupert
authors.forename: S
authors.initials: S
authors.lastname: Magil
authors.forename: R T
authors.initials: RT
authors.lastname: Gallagher
authors.forename: N
authors.initials: N
authors.lastname: Berova
authors.forename: K
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007031:Hypothalamus / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010042:Ouabain / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011487:Protein Conformation; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase