Double staining of skeleton using microwave irradiation.
The fetal skeleton double staining method is used to reveal developmental abnormalities in the skeletal system. We used alizarin red S and alcian blue successfully with microwave irradiation for skeletal double staining. The fixation time was reduced from 4-7 days to 2-2.5 min and the staining time was reduced from 4 days to 23 min.
Ilgaz
C
C
Department of Histology and Embryology, Gazi University, Ankara, Turkey.
Kocabiyik
H
H
Erdogan
D
D
Ozogul
C
C
Peker
T
T
D000818:Animals; D001842:Bone and Bones / Q000196:embryology*; D005260:Female; D008297:Male; D051379:Mice; D008872:Microwaves; D013194:Staining and Labeling / Q000379:methods*