title: p200, a collagen secreted by Schwann cells, is expressed in developing nerves and in adult nerves following axotomy.
abstract: Previously we reported that cultured rat Schwann cells secrete p200, a collagen-like heparin-binding adhesive glycoprotein with a restricted pattern of expression. Here we report that p200 is secreted as a stable trimer, but only after treatment of Schwann cells with ascorbic acid, and was deposited in the fibrillar extracellular matrix. Heparin and heparitinase treatment inhibited incorporation of p200 into extracellular matrix, suggesting the involvement of Schwann cell heparan sulfate proteoglycans in this process. Pepsin digestion revealed that p200 secreted by ascorbate-treated cells contains a collagenous domain of approximately 140 kDa. Immunofluorescent staining of rat embryos at different ages showed that p200 first appeared between embryonic days 15 and 18, and was confined to peripheral nerves. Staining of adult peripheral nerve was negative, but p200 expression was induced in adult sciatic nerve following nerve transection. These data suggest that p200 carries out unique functions during peripheral nerve development and regeneration and that its expression by Schwann cells is regulated by axon-Schwann cell interaction.
authors.affiliation: Henry Hood M.D. Research Program, Weis Center for Research, Pennsylvania State College of Medicine, Danville 17822-2613, USA. mchernousov@psghs.edu
authors.forename: M A
authors.initials: MA
authors.lastname: Chernousov
authors.forename: S S
authors.initials: SS
authors.lastname: Scherer
authors.forename: R C
authors.initials: RC
authors.lastname: Stahl
authors.forename: D J
authors.initials: DJ
authors.lastname: Carey
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001205:Ascorbic Acid / Q000494:pharmacology; D019771:Axotomy; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006493:Heparin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D008970:Molecular Weight; D009416:Nerve Regeneration; D010434:Pepsin A / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000196:embryology / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D011509:Proteoglycans; C036090:heparin proteoglycan; D006493:Heparin; D003094:Collagen; D010434:Pepsin A; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase; D001205:Ascorbic Acid