Comparison of restriction pattern polymorphism of Mycoplasma agalactiae and Mycoplasma bovis by pulsed field gel electrophoresis.
Mycoplasma agalactiae and Mycoplasma bovis are closely related species in phylogenetic terms. Pulsed Field Gel Electrophoresis (PFGE) of SmaI, EclXI, Bsi WI, MluI, BssHII, SalI, XhoI, NruI and ApaI digested DNAs were used to analyse and to compare restriction fragment length polymorphism between M. agalactiae and M. bovis and to estimate their genome sizes. SmaI, EclXI and Bsi WI enzymes cleaved DNAs of both microrganisms. MluI, BssHII, SalI, XhoI and NruI digested only M. agalactiae DNA whereas ApaI cut only M. bovis DNA. The total DNA length was established to be 945 +/- 8.4 Kb for M. agalactiae and 961 +/- 18.9 Kb for M. bovis.
Tola
S
S
Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, Sassari, Italy. setola@tin.it
Idini
G
G
Rocchigiani
A M
AM
Manunta
D
D
Angioi
P P
PP
Rocca
S
S
Cocco
M
M
Leori
G
G
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D016680:Genome, Bacterial; D006801:Humans; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004269:DNA, Bacterial