Interleukin-15 enhances host protection against acute Toxoplasma gondii infection in T-cell receptor alpha-/-deficient mice.
A potential role for T-cell receptor (TCR)-gamma(delta) cells in response to Toxoplasma gondii has been described. This study was undertaken to evaluate whether exogenous rIL-15 can enhance the production of gamma(delta) T cells in response to infection with T. gondii. For this, C57BL/6 and TCR alpha-gene deficient mice (alpha-/-) were vaccinated with Toxoplasma lysate antigen (TLA) in combination with interleukin (IL)-15. The cellular and humoral immune response was assessed following immunization. Administration of IL-15/TLA to alpha-/-mice lengthened survival time post lethal challenge. Several immunological parameters were increased in the alpha-/-mice following immunization with IL-15/TLA including serum immunoglobulin (Ig)G1 and IgG2a antibody titres and splenocyte lymphoproliferation in response to parasite antigen. Further, the CTL response to parasite infected target cells as well as the production of interferon gamma was enhanced by IL-15/TLA administration in the alpha-/-mice. These observations indicate that the gamma(delta) T cells response to this parasite can be enhanced by the administration of exogenous IL-15 in the presence of parasite lysate antigen.
Lee
Y H
YH
Departments of Medicine and Microbiology, Dartmouth Medical School, Lebanon, NH 03756, USA;
Ely
K H
KH
Lepage
A
A
Kasper
L H
LH
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D005260:Female; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D019409:Interleukin-15 / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D019409:Interleukin-15; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins; D007371:Interferon-gamma