Recombinant fusion protein and DNA vaccines against foot and mouth disease virus infection in guinea pig and swine.
In this study, we provide evidence that a recombinant fusion protein containing beta-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.
Huang
H
H
State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, China.
Yang
Z
Z
Xu
Q
Q
Sheng
Z
Z
Xie
Y
Y
Yan
W
W
You
Y
Y
Sun
L
L
Zheng
Z
Z
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D006168:Guinea Pigs; D007111:Immunity, Cellular / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000276:immunology; D010957:Plasmids / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D013552:Swine; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D010455:Peptides; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines; D001616:beta-Galactosidase