title: Anti-anisakis antibodies in the clinical course of Crohn's disease.
abstract: BACKGROUND
  Human anisakidosis is a parasitic disease whose intestinal form shares several characterictics with Crohn's disease (CD), like the ileocolonic location predominantly and the presence of granuloma.
  
  
  METHODS
  73 CD patients diagnosed according to Lennard-Jones' criteria were studied. The disease activity was measured by means of Harvey-Bradshaw Index. Different clinical variables were analyzed. Antigenic extract of Anisakis simplex larvae extracted from blue whiting was prepared. Determination of IgG, IgM, IgA and total specific immunoglobulin against A. simplex were carried out in sera by enzyme-linked immunosorbent assay. Immunorecognition patterns of these sera were realized by immunoblotting analysis. Results were compared with prevalence of antibodies anti-Anisakis in 251 healthy controls.
  
  
  RESULTS
  29% of CD patients showed detectable specific total Ig (G+M+A) against A. simplex (95% CI, 19-41). For IgG, IgM and IgA the percentages were 44, 18 and 53% (95% CI, 32-56, 10-26, and 41-65) respectively. In positive specific total Ig cases CD was localized mainly in ileum. In IgG-, IgM- and IgA-positive cases the location was predominantly ileocolonic. CD activity index was higher for groups with positive IgA (2.86 vs. 5.55; p < 0.05).
  
  
  CONCLUSIONS
  Patients with CD have high prevalence of specific immunoglobulin against A. simplex. Specific IgA are associated to higher activity index of CD.
authors.affiliation: Departamento de Parasitologia, Facultad de Farmacia, Universidad Complutense, Hospital la Paz, Madrid, España.
authors.forename: R
authors.initials: R
authors.lastname: Guillén-Bueno
authors.forename: R
authors.initials: R
authors.lastname: Gutiérrez-Ramos
authors.forename: M J
authors.initials: MJ
authors.lastname: Perteguer-Prieto
authors.forename: A
authors.initials: A
authors.lastname: Olveira-Martin
authors.forename: I
authors.initials: I
authors.lastname: Fernandez-Blanco
authors.forename: A
authors.initials: A
authors.lastname: Pozuelo-García
authors.forename: C
authors.initials: C
authors.lastname: Cuéllar del Hoyo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017129:Anisakiasis / Q000276:immunology* / Q000469:parasitology; D017163:Anisakis / Q000276:immunology*; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D003424:Crohn Disease / Q000276:immunology* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012307:Risk Factors; D012720:Severity of Illness Index
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth