title: Screening for protein-protein interactions.
abstract: The assays described above can be used to screen for cellular proteins that can interact with a protein of interest, to screen for mutant proteins that retain the ability to bind to its partner, and to identify the domains and amino acids involved in known protein-protein interactions. Nonetheless, the biological significance of some of these interactions needs to be confirmed by appropriate cellular studies.
authors.affiliation: University of Medical Dentistry of New Jersey, Clinical Institute of New Jersey, New Brunswick 08901, USA.
authors.forename: F J
authors.initials: FJ
authors.lastname: Germino
authors.forename: N K
authors.initials: NK
authors.lastname: Moskowitz
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods*; D017124:Conserved Sequence; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000096:biosynthesis; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D005399:Fishes; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000426:Alcohol Dehydrogenase; D001616:beta-Galactosidase