title: Multicenter validation of recombinant, natural and synthetic antigens used in a single multiparameter assay for the detection of specific anti-nuclear autoantibodies in connective tissue disorders.
abstract: OBJECTIVE
  We investigated the feasibility of using a single multi-parameter test based mainly on recombinant autoantigens for the detection of anti-nuclear autoantibodies, and analyzed the agreement between this test format and conventional techniques.
  
  
  METHODS
  The presence of autoantibodies was determined by a line immunoassay (LIA) in 755 sera derived from patients with different autoimmune connective tissue disorders. All sera were previously tested by standard assays that are routinely used at the 8 participating European centers.
  
  
  RESULTS
  The overall sensitivity and specificity of autoantibody detection by LIA was similar or higher as compared to combined conventional techniques (CCT). In particular, the detection of anti-Ro52 in systemic lupus erythematosus (SLE) sera (P = 0.004) and anti-LA in both SLE (P < 0.0009) and in Sjögren's syndrome (P < 0.0009) sera was significantly more sensitive when using LIA compared to CCT. By contrast, CCT was never more sensitive than LIA for any of the markers.
  
  
  CONCLUSIONS
  The LIA is a reliable alternative to a combination of conventional techniques for the detection of specific anti-nuclear autoantibodies. The multi-parameter test also reveals autoantibody reactivities that may not be detected when only a limited number of conventional techniques are applied.
authors.affiliation: Innogenetics N.V., Ghent, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Meheus
authors.forename: W J
authors.initials: WJ
authors.lastname: van Venrooij
authors.forename: A
authors.initials: A
authors.lastname: Wiik
authors.forename: P J
authors.initials: PJ
authors.lastname: Charles
authors.forename: A G
authors.initials: AG
authors.lastname: Tzioufas
authors.forename: O
authors.initials: O
authors.lastname: Meyer
authors.forename: G
authors.initials: G
authors.lastname: Steiner
authors.forename: D
authors.initials: D
authors.lastname: Gianola
authors.forename: S
authors.initials: S
authors.lastname: Bombardieri
authors.forename: A
authors.initials: A
authors.lastname: Union
authors.forename: S
authors.initials: S
authors.lastname: De Keyser
authors.forename: E
authors.initials: E
authors.lastname: Veys
authors.forename: F
authors.initials: F
authors.lastname: De Keyser
mesh_terms: D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D001324:Autoantigens / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000276:immunology*; D005240:Feasibility Studies; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015151:Immunoblotting; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; D011993:Recombinant Fusion Proteins