title: Antigen-specific in vitro activation of T-lymphocyte subsets of cattle immunized with a modified live bovine herpesvirus 1 vaccine.
abstract: Bovine peripheral blood mononuclear cells from cattle immunized with a modified live virus bovine herpesvirus 1 (BHV1) vaccine and from BHV1-negative cattle were incubated in vitro with inactivated BHV1 for 6 days. Activation of T-lymphocyte subsets was measured by two-color flow cytometric analysis of T-cell phenotype and surface expression of CD25, the alpha-subunit of the high-affinity interleukin-2 receptor. Vaccinated animals, but not unvaccinated animals, had CD3+, CD4+, and gamma-delta T cells that significantly (p < 0.05) increased expression of CD25 when incubated with BHV1. CD8+ T cells from vaccinated animals did not consistently increase CD25 expression when incubated with inactivated BHV1.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Quade
authors.forename: J A
authors.initials: JA
authors.lastname: Roth
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D002417:Cattle; D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005434:Flow Cytometry; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D018984:Epitopes, T-Lymphocyte; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D014613:Vaccines, Attenuated; D014765:Viral Vaccines