title: [Inhibition of peritoneal bacterial adhesion using oligosaccharides. An experimental model of peritonitis in rats].
abstract: BACKGROUND
  Peritoneal colonization is a crucial event in the pathogenesis of peritonitis and its local complications. Adherence to the serosal mesothelium is mediated in a number of microorganisms derived from the digestive tract (especially E. coli) by type-1 fimbriae which have an oligosaccharide specificity.
  
  
  OBJECTIVE
  To evaluate the effect of repeated peritoneal washes with saline solution and oligosaccharides on E. coli peritoneal adherence in a rat peritonitis model.
  
  
  METHODS
  Sixty rats were randomized in 3 groups of 20. E. coli was inoculated at a constant concentration of 10(8)/mL per 100 g of weight. Then, peritoneal washes were achieved daily during three consecutive days (D1, D2, D3), with saline solution in Group I (control group), Methyl alpha-D-Mannoside (MADM) in Group II, and p-Nitro-phenyl alpha-D-Mannoside (pNADM) in Group III. Peritoneal samples were obtained before and after lavage at D1, D2, and D3. Microbial recovery was expressed as cfu/mg of tissue, and converted into a percentage of the initial value. A 10% threshold defined efficiency of the wash (inhibition of adherence for 90% of bacteries).
  
  
  RESULTS
  Compared with data from Group I, E. coli peritoneal adherence was significantly lower after washes in Group III (D1: p = 0.03; D2: p = 0.009; D3: p = 0.003). Repeated washes were more efficient in Group III than in Group II (D1: p = 0.1; D2: p = 0.5; D3: p = 0.001).
  
  
  CONCLUSIONS
  These results suggest that the addition of oligosaccharides, especially of pNADM, reduces the peritoneal adherence of E. coli when a peritoneal wash is performed for peritonitis.
authors.affiliation: Service de chirurgie digestive et générale, hôpital Sainte-Marguerite, Marseille, France.
authors.forename: I
authors.initials: I
authors.lastname: Sielezneff
authors.forename: M N
authors.initials: MN
authors.lastname: Mallet
authors.forename: P
authors.initials: P
authors.lastname: Berthezene
authors.forename: B
authors.initials: B
authors.lastname: Sastre
authors.forename: J C
authors.initials: JC
authors.lastname: Dagorn
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D004927:Escherichia coli Infections / Q000188:drug therapy*; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000502:physiology; D008362:Mannosides / Q000627:therapeutic use*; D008766:Methylmannosides; D010533:Peritoneal Lavage; D010537:Peritoneum / Q000382:microbiology; D010538:Peritonitis / Q000188:drug therapy / Q000382:microbiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride; D013501:Surface-Active Agents / Q000627:therapeutic use*
chemical_list: D008362:Mannosides; D008766:Methylmannosides; D013501:Surface-Active Agents; C002731:4-nitrophenyl-alpha-D-mannopyranoside; D012965:Sodium Chloride; C008466:methylmannoside