title: Effect of garlic active principle, diallyl disulfide, on cell viability, lipid peroxidation, glutathione concentration and its related enzyme activities in primary rat hepatocytes.
abstract: This study investigated the effects of various concentrations and incubation time intervals of diallyl disulfide (DADS), the active principle of garlic, on: 1. cell viability, 2. lipid peroxidation, and 3. glutathione (GSH) concentration and its related enzyme activities of rat hepatocytes. According to the results of LDH leakage and microscopic examination, 0.5 and 1 mM DADS did not significantly affect the viability of hepatocytes. However, significant decrease in cell viability according to increased LDH leakage and significant changes in morphology of hepatocytes were observed at 2 mM DADS (P < 0.05). Lipid peroxidation was also detected when the hepatocytes were treated with 2 mM DADS. At 0.5 mM DADS, a higher GSH content was found in the hepatocytes although not at a statistically significant level. 0.5 and 1 mM DADS has little effect on the activities of glutathione-S-transferase (GST) and glutathione peroxidase (GPx); however a significant decrease in GST, GPx and glutathione reductase (GRd) activities was observed at 2 mM DADS. Once the media of 2 mM DADS was replaced with fresh medium at 24 hr treatment, the activities of GST, GRd and GPx were recovered, although they were still lower than the control values.
authors.affiliation: Department of Nutrition, China Medical College, Taichung, Taiwan.
authors.forename: L Y
authors.initials: LY
authors.lastname: Sheen
authors.forename: S F
authors.initials: SF
authors.lastname: Sheu
authors.forename: S J
authors.initials: SJ
authors.lastname: Tsai
authors.forename: R H
authors.initials: RH
authors.lastname: Meng
authors.forename: C K
authors.initials: CK
authors.lastname: Lii
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004220:Disulfides / Q000494:pharmacology*; D005737:Garlic; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D010938:Plant Oils / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000498:Allyl Compounds; D000970:Antineoplastic Agents; D004220:Disulfides; D010938:Plant Oils; C028009:diallyl disulfide; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione