[The interrelationship of biomass and toxin accumulation to the dynamics of the physicochemical parameters of Corynebacterium diphtheriae PW8 cultivation].
As established in the process the controlled cultivation of C. diphtheriae PW8, the rate of the accumulation of biomass and antigenic material increases with higher values of the redox potential of the nutrient medium (exceeding 11.0 mV). The data obtained in this study are indicative of the expediency of using the value of redox potential as an additional criterion in the evaluation of the quality of the nutrient media. The direct relationship between the productivity of the process of C. diphtheriae PW8 cultivation as regards the accumulation of biomass and antigenic material and the economic coefficients of the accumulation of biomass and antigenic material as determined by the utilized substrate has been established. The above relationship may facilitate the prognostication of the course of the process of microbial cultivation and the accumulation of diphtheria toxin, evaluated by antigenic activity.
Gruber
I M
IM
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Dmitrieva
M N
MN
Gavrilova
N A
NA
D000942:Antigens, Bacterial / Q000032:analysis / Q000096:biosynthesis; D001431:Bacteriological Techniques; D018533:Biomass; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003353:Corynebacterium diphtheriae / Q000737:chemistry / Q000254:growth & development* / Q000276:immunology; D003470:Culture Media; D004167:Diphtheria Toxin / Q000032:analysis / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D018709:Statistics, Nonparametric
D000942:Antigens, Bacterial; D003470:Culture Media; D004167:Diphtheria Toxin