[The isolation of monoclonal antibodies to the hemolysin of noncholerigenic vibrios].
The results of the work on obtaining monoclonal antibodies (McAb) to hemolysin of noncholerigenic vibrios and the study of hemolysin preparations and Vibrio cholerae strains are presented. As established with the use of McAb, hemolysin preparations proved to be complex compounds containing, in addition to hemolytic protein, some lipopolysaccharide determinants, which had not been detected with the use of traditional biochemical tests. The study revealed that V. cholerae non-01 and V. cholerae eltor hemolysins have common antigenic determinants, which was confirmed by the data of literature on the homogeneity of these substances. McAb obtained in this study interacted with V. cholerae avirulent hemolytic strains, not interacting with V. cholerae classical strains.
Telesmanich
N R
NR
Research Institute for Plague Control, Rostov-on-Don, Russia.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D006460:Hemolysin Proteins