title: Comparison between 2 test models in evaluating the effect of a moisturizer on irritated human skin.
abstract: The purpose of the present study was to compare 2 experimental models of moisturizer efficacy on the recovery of irritated skin on the hands and the volar forearms. 12 healthy volunteers had their hands immersed in sodium lauryl sulfate (SLS) 10 min 2x daily for 2 days, and at the same time the volunteers had patch tests with SLS (0.125%, 0.25% and 0.5%) applied on their forearms for 24 h. After irritation of the skin, the volunteers had a moisturizer applied on one arm/hand 3x daily for the following 9 days. The other arm/hand served as untreated control. Evaluation was done on days (D) 1, 3, 5, 8 and 12 by transepidermal water loss, electrical capacitance, laser Doppler flowmetry and DermaSpectrometry. Both models were found useful, and the moisturizer was found to accelerate regeneration of the skin barrier function in both the hands (D8, p<0.05) and the volar forearms (0.5% SLS, D5 and D8, p<0.01). When the forearm model is used in the present set-up, a relatively high concentration of SLS (>0.25%) should be used and evaluation measurements are best performed on D5-D8. The forearm model proved reliable and easy to handle and we suggest that this model is used in future studies on moisturizer evaluation.
authors.affiliation: Department of Dermatology, University of Copenhagen, Gentofte Hospital, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Held
authors.forename: T
authors.initials: T
authors.lastname: Agner
mesh_terms: D000328:Adult; D017453:Dermatitis, Irritant / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D004643:Emollients / Q000494:pharmacology / Q000627:therapeutic use*; D004890:Erythema / Q000473:pathology; D005260:Female; D005542:Forearm; D006229:Hand Dermatoses / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D006801:Humans; D017078:Laser-Doppler Flowmetry; D008297:Male; D008875:Middle Aged; D010328:Patch Tests; D012720:Severity of Illness Index; D016037:Single-Blind Method; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects; D013501:Surface-Active Agents / Q000009:adverse effects; D016896:Treatment Outcome; D014870:Water Loss, Insensible / Q000187:drug effects
chemical_list: D004643:Emollients; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate