Existence of human DAZLA protein in the cytoplasm of human oocytes.
The human deleted in azoospermia-like autosomal (DAZLA) gene is thought to be a candidate gene for azoospermia. cDNA encoding the C-terminal 94 amino acid residues of human DAZLA was used to express a bacterial fusion protein which was then used to raise polyclonal antibodies in rabbits. Immunohistochemical analyses with the human DAZLA antiserum showed that the DAZLA protein is expressed at a cytoplasmic location in female germ cells. Available evidence suggests that the DAZLA gene is a participant in human oogenesis.
Nishi
S
S
Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
Hoshi
N
N
Kasahara
M
M
Ishibashi
T
T
Fujimoto
S
S
D000293:Adolescent; D000328:Adult; D000818:Animals; D015153:Blotting, Western; D003593:Cytoplasm / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008593:Menopause; D008875:Middle Aged; D009865:Oocytes / Q000378:metabolism*; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D016601:RNA-Binding Proteins; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013737:Testis / Q000378:metabolism
C101358:DAZL protein, human; D011506:Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins