Cloning and molecular characterization of the citrate utilization citMCDEFGRP cluster of Leuconostoc paramesenteroides.
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF the subunits of the citrate lyase of Leuconostoc. Moreover, it suggests that citM encodes a Leuconostoc malic enzyme. Analysis of citrate consumption by and citrate lyase activity of Lc. paramesenteroides J1[pCITJ1] showed that its citrate permease and its citrate lyase are induced by the presence of citrate in the growth medium. Southern blot analysis demonstrated that the citMCDEFGRP cluster is located in a plasmid.
Martín
M
M
Centro de Investigaciones Biológicas (CSIC), Madrid, Spain.
Corrales
M A
MA
de Mendoza
D
D
López
P
P
Magni
C
C
D001426:Bacterial Proteins; D001483:Base Sequence; D019343:Citric Acid / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D027361:Organic Anion Transporters; D007652:Oxo-Acid-Lyases / Q000235:genetics* / Q000378:metabolism; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D009097:Multienzyme Complexes; D027361:Organic Anion Transporters; D019343:Citric Acid; C065431:citP protein, Lactococcus lactis; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase