Bovine herpes virus expressing envelope protein (E2) of bovine viral diarrhea virus as a vaccine candidate.
The gene encoding the envelope protein (E2) of bovine viral diarrhea virus (BVDV) was expressed under the thymidine kinase (TK) promoter of Korean bovine herpesvirus 1 (BHV-1) isolate. Thymidine kinase negative (TK-) BHV-1 recombinants expressing E2 of BVDV were constructed and the expression of E2 was identified by immunofluorescence and Western blotting. Compared to wild type BHV-1, the recombinant BHV-1 had a delayed cytopathogenic effect in cells. The immunogenicity of the recombinant BHV-1 was examined in guinea pigs and cattle. Although an increase in body temperature was detected for a few days, the inoculated cattle returned to normal temperature with the development of neutralizing antibodies to BVDV.
Kweon
C H
CH
National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Republic of Korea.
Kang
S W
SW
Choi
E J
EJ
Kang
Y B
YB
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015153:Blotting, Western / Q000662:veterinary; D001831:Body Temperature; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control*; D002417:Cattle; D002472:Cell Transformation, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005822:Genetic Vectors; D006168:Guinea Pigs; D007242:Herpesvirus 1, Bovine; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C076551:gp53, bovine viral diarrhea virus; D013937:Thymidine Kinase