title: A novel electron transfer mechanism suggested by crystallographic studies of mitochondrial cytochrome bc1 complex.
abstract: The crystal structure of bovine mitochondrial cytochrome bc1 complex, an integral membrane protein complex of 11 different subunits with a total molecular mass of 242 kDa, demonstrated a tightly associated dimer consisting of three major regions: a matrix region primarily made of subunits core1, core2, 6, and 9; a transmembrane-helix region of 26 helices in the dimer contributed by cytochrome b, cytochrome c1, the Rieske iron-sulfur protein (ISP), subunits 7, 10, and 11; and an intermembrane-space region composed of extramembrane domains of ISP, cytochrome c1, and subunit 8. The structure also revealed the positions of and distances between irons of prosthetic groups, and two symmetry related cavities in the transmembrane-helix region upon dimerization of the bc1 complex. Extensive crystallographic studies on crystals of bc1 complexed with inhibitors of electron transfer identified binding pockets for both Qo and Qi site inhibitors. Discrete binding sites for subtypes of Qo site inhibitors have been mapped onto the Qo binding pocket, and bindings of different subtypes of Qo site inhibitors are capable of inducing dramatic conformational changes in the extramembrane domain of ISP. A novel electron transfer mechanism for the bc1 complex consistent with crystallographic observations is discussed.
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas 75235, USA. dixia@helix.nih.gov
authors.forename: D
authors.initials: D
authors.lastname: Xia
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: C A
authors.initials: CA
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: A
authors.initials: A
authors.lastname: Kachurin
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Deisenhofer
mesh_terms: D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray; D004579:Electron Transport / Q000502:physiology*; D014450:Electron Transport Complex III / Q000737:chemistry*; D008928:Mitochondria / Q000737:chemistry*; D008954:Models, Biological; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D014450:Electron Transport Complex III