An approach for high sensitivity detection of prostate cancer by analysis of changes in structuredness of the cytoplasmic matrix of lymphocytes specifically induced by PSA-ACT.
OBJECTIVE
An alternative procedure for detection of prostate cancer was examined based on the observation that cells reexposed in vitro to antigenic or mitogenic stimulation will change their intracellular structuredness as measured by polarization of fluorescent light emitted by labeled cells (SCM test).


METHODS
Lymphocytes derived from patients bearing a nonmalignant prostate tumor and healthy individuals were exposed to PSA-ACT, PHA, and MUC-1.


RESULTS
Of sixty-five patients with prostate carcinoma (CaP), sixty-two were correctly diagnosed by the test. Of the eighty males in the control group, five were incorrectly diagnosed as having the disease and seventy-five were correctly diagnosed as healthy subjects. The sensitivity of the test was 96.8%. The specificity was 91.1%. The BPH (Benign Prostatic Hyperplasia) control group exhibited a sensitivity of 9.38%, but the specificity was 91.1%. Similar percentages for specificity and sensitivity were observed in the NRT (Non-Relevant Tumor) control group.


CONCLUSIONS
The results shown here indicate the possibility of a different use of PSA-ACT for detection of prostate cancer with high specificity and sensitivity.
Klein
O
O
Urology Department, Rebecca Sieff Medical Center, Tzfat, Israel.
Lin
S
S
Embon
O
O
Sazbon
A
A
Zidan
J
J
Kook
A I
AI
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm; D003593:Cytoplasm / Q000473:pathology*; D005454:Fluorescence Polarization; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D018396:Mucin-1; D009077:Mucins; D010835:Phytohemagglutinins; D017430:Prostate-Specific Antigen; D011471:Prostatic Neoplasms / Q000473:pathology*; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; C060192:MUC1 protein, human; D018396:Mucin-1; D009077:Mucins; D010835:Phytohemagglutinins; D017430:Prostate-Specific Antigen