Physical mapping of the CXC chemokine locus on human chromosome 4.
A physical map of the CXC chemokine locus on chromosome 4 has been constructed by PCR analysis and PFGE mapping of YAC clones. The genes for IL8, GRO1, PPBP, PF4, SCYB5 (ENA-78) and SCYB6 (GCP-2) have been co-localized on a 335-kb genomic fragment. The GRO2 and GRO3 genes did not map within this region and based on analysis of a YAC contig overlapping IL8 we speculate that GRO2 and GRO3 map downstream of this region. We have also assigned the novel CXC chemokine gene, SCYB9B (alias H174/betaR1) to chromosome 4q21, upstream and within 12 kb of INP10. Like INP10 and MIG, INP10 and SCYB9B are arranged in a head to tail manner. The chromosomal arrangement of these genes appears to reflect the evolution of this multigene family and supports the theory that it arose by gene duplication.
O'Donovan
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Department of Microbiology, University College Cork, Cork, Ireland.
Galvin
M
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Morgan
J G
JG
D005075:Biological Evolution; D054360:Chemokine CXCL1; D054365:Chemokine CXCL5; D054427:Chemokine CXCL6; D019743:Chemokines, CXC / Q000235:genetics*; D002630:Chemotactic Factors / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D020440:Gene Duplication; D006133:Growth Substances / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000031:analogs & derivatives / Q000235:genetics; D005810:Multigene Family; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D010978:Platelet Factor 4 / Q000235:genetics; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
C475829:CXCL1 protein, human; C499707:CXCL3 protein, human; C486263:CXCL5 protein, human; C515503:CXCL6 protein, human; D054360:Chemokine CXCL1; D054365:Chemokine CXCL5; D054427:Chemokine CXCL6; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D010978:Platelet Factor 4