Increasing DNA transfer efficiency by temporary inactivation of host restriction.
E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restriction of incoming DNA by the recipient cells. Here, we describe a simple method that temporarily inactivates host restriction, allowing high-frequency DNA transfer. This technique might be readily applied to a wide range of bacteria to increase DNA transfer between strains and species.
Edwards
R A
RA
Department of Microbiology, University of Illinois, Urbana-Champaign 61801, USA.
Helm
R A
RA
Maloy
S R
SR
D017100:Bacteriophage P22 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D001709:Biotechnology; D003227:Conjugation, Genetic; D004274:DNA, Recombinant / Q000235:genetics*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000037:antagonists & inhibitors; D018274:Electroporation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D018014:Gene Transfer Techniques; D006358:Hot Temperature; D012477:Salmonella enteritidis / Q000201:enzymology / Q000235:genetics; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics; D013045:Species Specificity
D004274:DNA, Recombinant; D015253:Deoxyribonucleases, Type I Site-Specific