title: Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum.
abstract: A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Korea. hslee@wonnms.wonkwang.ac.kr
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: S B
authors.initials: SB
authors.lastname: Kim
authors.forename: B E
authors.initials: BE
authors.lastname: Choi
authors.forename: B H
authors.initials: BH
authors.lastname: Woo
authors.forename: K C
authors.initials: KC
authors.lastname: Lee
mesh_terms: D006801:Humans; D007723:Korea; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D014784:Mistletoe / Q000737:chemistry*; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: D037102:Lectins; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum