title: Simplified PCR set-up using a frozen preformulated mix for the detection of cytomegalovirus.
abstract: A simple PCR set-up for the detection of cytomegalovirus in clinical specimen was developed. All components of the PCR master mix including Taq DNA polymerase, uracil N-glycosilase, and primers were preformulated and stored frozen in aliquots. After thawing the master mix aliquots, the PCR was immediately started after the addition of sample DNA. This method gave excellent reproducible PCR-results without loss of enzyme activities following storage at -20 degrees C for at least 4 months.
authors.affiliation: Children's Hospital, General Hospital Salzburg, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Kofler
authors.forename: A
authors.initials: A
authors.lastname: Klausegger
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D045647:DNA Glycosylases; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D005615:Freezing; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase / Q000378:metabolism; D051981:Uracil-DNA Glycosidase
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase