title: Separation and identification of corticosterone metabolites by liquid chromatography--electrospray ionization mass spectrometry.
abstract: High-performance liquid chromatography coupled to atmospheric pressure ionization-electrospray ionization mass spectrometry (API-ESI-MS) was investigated for the analysis of corticosterone metabolites; their characterization was obtained by combining the separation on Zorbax Eclipse XDB C18 column (eluted with a methanol-water-acetic acid gradient) with identification using positive ion mode API-ESI-MS and selected ion analysis. The applicability of this method was verified by monitoring the activity of steroid converting enzymes (20beta-hydroxysteroid dehydrogenase and 11beta-hydroxysteroid dehydrogenase) in avian intestines.
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Prague. miksik@biomed.cas.cz
authors.forename: I
authors.initials: I
authors.lastname: Miksík
authors.forename: M
authors.initials: M
authors.lastname: Vylitová
authors.forename: J
authors.initials: J
authors.lastname: Pácha
authors.forename: Z
authors.initials: Z
authors.lastname: Deyl
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000302:isolation & purification / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003345:Corticosterone