title: cpmA, a gene involved in an output pathway of the cyanobacterial circadian system.
abstract: We generated random mutations in Synechococcus sp. strain PCC 7942 to look for genes of output pathways in the cyanobacterial circadian system. A derivative of transposon Tn5 was introduced into the chromosomes of reporter strains in which cyanobacterial promoters drive the Vibrio harveyi luxAB genes and produce an oscillation of bioluminescence as a function of circadian gene expression. Among low-amplitude mutants, one mutant, tnp6, had an insertion in a 780-bp open reading frame. The tnp6 mutation produced an altered circadian phasing phenotype in the expression rhythms of psbAI::luxAB, psbAII::luxAB, and kaiA::luxAB but had no or little effect on those of psbAIII::luxAB, purF::luxAB, kaiB::luxAB, rpoD2::luxAB, ndhD::luxAB, and conII::luxAB. This suggests that the interrupted gene in tnp6, named cpmA (circadian phase modifier), is part of a circadian output pathway that regulates the expression rhythms of psbAI, psbAII, and kaiA.
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843-3258, USA.
authors.forename: M
authors.initials: M
authors.lastname: Katayama
authors.forename: N F
authors.initials: NF
authors.lastname: Tsinoremas
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: S S
authors.initials: SS
authors.lastname: Golden
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001683:Biological Clocks / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D056925:Circadian Rhythm Signaling Peptides and Proteins; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D045332:Photosystem II Protein Complex; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D056925:Circadian Rhythm Signaling Peptides and Proteins; D004251:DNA Transposable Elements; C114269:KaiA protein, cyanobacteria; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C119580:cpmA protein, Synechococcus sp.