Genomic analysis of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 in human pancreatic ductal adenocarcinoma.
CONCLUSIONS
Abnormality of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 appears to play a limited role in pancreatic ductal carcinogenesis.


BACKGROUND
Recently, a human G/T-specific TDG gene was identified. This protein acts in a system correcting G/T mispairs to G/C pairs. TDG was mapped to chromosome bands 12q22-q24.1, one of the regions frequently lost in pancreatic cancer. Therefore, there is the possibility that the TDG gene on 12q is one of the genes responsible for pancreatic ductal carcinogenesis.


METHODS
Nucleotide sequences of the entire coding region of the TDG gene were analyzed in 21 human pancreatic cancer cell lines. mRNA expression of the TDG gene was also analyzed by Northern hybridization in several human tissues and 21 human pancreatic cancer cell lines.


RESULTS
Decreased levels of mRNA expression were detected in the pancreatic cancer cell lines, but no somatic mutations were observed.
Yatsuoka
T
T
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
Furukawa
T
T
Abe
T
T
Yokoyama
T
T
Sunamura
M
M
Kobari
M
M
Matsuno
S
S
Horii
A
A
D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D015894:Genome, Human; D006801:Humans; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)