Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake.
An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3-4-5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7, 8-diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7-keto 8-aminopelargonic acid.
Phalip
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V
Laboratoire de Biotechnologie, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brandt, 67400, Illkirch-Graffenstaden, France. vincent.phalip@esbs.u-strasbg.fr
Kuhn
I
I
Lemoine
Y
Y
Jeltsch
J M
JM
D000596:Amino Acids / Q000378:metabolism*; D000599:Amino Acids, Diamino / Q000378:metabolism; D001692:Biological Transport; D001710:Biotin / Q000096:biosynthesis* / Q000235:genetics; D019731:Carbon-Nitrogen Ligases / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D000637:Transaminases / Q000235:genetics / Q000378:metabolism
D000596:Amino Acids; D000599:Amino Acids, Diamino; C511615:BIO5 protein, S cerevisiae; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C004720:7,8-diaminopelargonic acid; C001502:7-keto-8-aminopelargonic acid; D001710:Biotin; C020029:7,8-diaminopelargonic acid aminotransferase; D000637:Transaminases; D019731:Carbon-Nitrogen Ligases; C020024:dethiobiotin synthetase