The N-terminus of collagenase MMP-8 determines superactivity and inhibition: a relation of structure and function analyzed by biomolecular interaction analysis.
Tissue inhibitors of metalloproteinases (TIMPs) are the physiological, specific inhibitors of matrix metalloproteinases (MMPs) forming tight, noncovalent complexes. Therefore they control the proteolytic activity of MMPs toward the extracellular matrix. To analyze the inhibition of the "activated" and "superactivated" variants of human neutrophil collagenase (MMP-8) by TIMP-2, we determined complex dissociation constants using biomolecular interaction analysis (BIA). As it is known that the association rate constants can exceed the limits of the BIA instruments, the biomolecular interaction analysis was used to examine the equlibrium situation. The dissociation constants were determined by fitting the parameters of the mathematical term for the binding of collagenase onto the TIMP-coupled sensor chip surface to the saturation curve derived from individual sensorgrams. The resulting values are in the nanomolar range and correlate with the results of fluorescence kinetics. These data reveal that TIMP-2 (the recombinant inhibitory domain of human TIMP-2 and bovine TIMP-2 isolated from seminal plama) is a better inhibitor of the activated neutrophil collagenase than of the superactivated variant (the recombinant catalytic domain of human MMP-8). It has been demonstrated by X-ray analysis that the N-terminal heptapeptide only of superactivated MMP-8 is attached by a salt bridge and hydrophobic interaction to the C-terminal helix. Because these interactions have to be disrupted in the complex formation with TIMP we assume that the activated variant enables higher flexibility and a tighter induced fit in the complex formation. Therefore superactivation of MMP-8 correlates with weaker inhibition by TIMP-2.
Farr
M
M
University of Bielefeld, Faculty of Chemistry and Biochemistry, Germany.
Pieper
M
M
Calvete
J
J
Tschesche
H
H
D000818:Animals; D002384:Catalysis; D002417:Cattle; D017364:Collagenases / Q000737:chemistry* / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances; D020784:Matrix Metalloproteinase 8; D061965:Matrix Metalloproteinase Inhibitors; D008956:Models, Chemical; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity / Q000235:genetics; D020349:Surface Plasmon Resonance; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D005456:Fluorescent Dyes; D008024:Ligands; D046911:Macromolecular Substances; D061965:Matrix Metalloproteinase Inhibitors; D010446:Peptide Fragments; D011994:Recombinant Proteins; D019716:Tissue Inhibitor of Metalloproteinase-2; D017364:Collagenases; D020784:Matrix Metalloproteinase 8