Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene.
Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the beta-glucuronidase (gusA) reporter gene. Subsequently, seeds were treated with EMS to obtain mutants in which both reporter gene fusions were up-regulated. Northern analysis showed that the mRNA level of a related, endogenous auxin-inducible GST gene of Arabidopsis was increased in some of these mutants as well. Two of the gup (GST up-regulated) mutants were characterized in more detail and roughly mapped. Both had epinastic cotyledons and leaves, a phenotype that turned out to be linked to the gup mutation.
van der Kop
D A
DA
Institute of Molecular Plant Sciences and RUL-TNO Centre for Phytotechnology, Leiden University, The Netherlands.
Schuyer
M
M
Pinas
J E
JE
van der Zaal
B J
BJ
Hooykaas
P J
PJ
D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010641:Phenotype; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015854:Up-Regulation
D004269:DNA, Bacterial; D007210:Indoleacetic Acids; D009153:Mutagens; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D005020:Ethyl Methanesulfonate; D005982:Glutathione Transferase