title: Simple version of "megaprimer" PCR for site-directed mutagenesis.
authors.affiliation: Gene Therapy Core Center, University of California, San Francisco 94143, USA.
authors.forename: A
authors.initials: A
authors.lastname: Colosimo
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: G
authors.initials: G
authors.lastname: Novelli
authors.forename: B
authors.initials: B
authors.lastname: Dallapiccola
authors.forename: D C
authors.initials: DC
authors.lastname: Gruenert
mesh_terms: D001483:Base Sequence; D001709:Biotechnology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D005069:Evaluation Studies as Topic; D006801:Humans; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: C505032:CFTR protein, human; D017931:DNA Primers; D019005:Cystic Fibrosis Transmembrane Conductance Regulator