CD36 LIMPII analogous-1, a human homolog of the rodent scavenger receptor B1, provides the cholesterol ester for steroidogenesis in adrenocortical cells.
CD36 and LIMPII analogous-1 (CLA-1), a human homolog of the rodent scavenger receptor B1 (SR-B1), binds high-density lipoprotein (HDL) and mediates the selective uptake of HDL cholesterol ester (CE) by cultured transfected cells. CLA-1 is strongly expressed in steroidogenic tissues, including the adrenal gland, suggesting that CLA-1 plays a role in providing substrates for steroidogenesis. To address this, we established an adrenocortical cell line that highly expresses CLA-1. These cells increased CE uptake from HDL to 140.5% of the level in mock-transfected cells. After incubation of the transfected cells with HDL, corticosterone secretion from CLA-1-transfected cells increased to about two times the level in mock-transfected cells. These results indicate the possibility that CLA-1 (a close structural homolog of SR-B1)-mediated uptake of HDL CE may be a significant source of precursor cholesterol for steroidogenesis in humans as it is in mice.
Imachi
H
H
First Department of Internal Medicine, Kagawa Medical University, Kita-gun, Japan.
Murao
K
K
Sato
M
M
Hosokawa
H
H
Ishida
T
T
Takahara
J
J
D000302:Adrenal Cortex / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism* / Q000493:pharmacokinetics; D003345:Corticosterone / Q000096:biosynthesis*; D006801:Humans; D008075:Lipoproteins, HDL / Q000493:pharmacokinetics; D051379:Mice; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein / Q000378:metabolism*; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D014162:Transfection; D014407:Tumor Cells, Cultured
D018955:CD36 Antigens; D002788:Cholesterol Esters; C059714:HDL cholesteryl ester; D008075:Lipoproteins, HDL; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D003345:Corticosterone