Evaluation of CAPTURE CMV solid phase testing over the allowable shelf-life of red blood cells (adenine-saline added).
BACKGROUND
CAPTURE CMV (IMMUCOR Inc., Norcross, GA) is a solid-phase screening test used to detect IgM and IgG antibodies to cytomegalovirus (CMV). CAPTURE CMV is licensed for testing whole blood (WB) in citrate phosphate dextrose (CPD) preserved segments of units of red blood cells (RBC) only up to 7 days of storage. We determined if CAPTURE CMV could produce consistent results in CPD preserved WB segments from RBC adenine-saline added (ASA) for their 42 day shelf-life.


METHODS
Ten CMV-seropositive and 10 CMV-seronegative RBC (ASA) tested by CAPTURE CMV during the first week of storage were studied. Segments were tested weekly for 6 weeks.


RESULTS
All 10 units that initially tested as CMV-seropositive remained strongly seropositive. All 10 units initially CMV-seronegative, remained seronegative.


CONCLUSIONS
CAPTURE CMV testing provides consistent results over the entire shelf-life of RBC (ASA).
Gammon
R R
RR
Clinical Laboratories and Blood Bank, University of Virginia, Charlottesville 22908, USA.
Avery
N
N
Mintz
P D
PD
D000225:Adenine; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D000925:Anticoagulants; D000937:Antigen-Antibody Reactions; D001793:Blood Preservation; D002951:Citrates; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis*; D004912:Erythrocytes; D005947:Glucose; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D007163:Immunosorbent Techniques; D008403:Mass Screening / Q000379:methods*; D010949:Plasma / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012965:Sodium Chloride; D013997:Time Factors
D000914:Antibodies, Viral; D000925:Anticoagulants; D002951:Citrates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D012965:Sodium Chloride; C007077:citrate phosphate dextrose; D005947:Glucose; D000225:Adenine