[Lowering of resistance to oxidation of VLDL lipoproteins and some subfractions of LDL lipoproteins in patients with non-insulin-dependent diabetes mellitus].
OBJECTIVE
To investigate composition of very low density lipoproteins (VLDLP) and some subfractions of low density lipoproteins (LDLP), and their resistance to oxidation in patients with non-insulin-dependent diabetes mellitus (NIDDM) varying in severity compared to healthy subjects.


METHODS
26 NIDDM patients and 16 healthy controls. VLDLP and subfractions of LDLP were isolated by ultracentrifugation. The lipoproteins were assayed for protein, cholesterol, initial products of lipid peroxidation (LPO). After autooxidation of lipoproteins, fluorimetry examined the degree of the oxidant modification.


RESULTS
The initial level of LPO products was elevated in VLDLP and light subfractions of LDLP in severe and moderate NIDDM patients. Even in mild NIDDM, resistance to oxidation of dense LDLP subfractions was low and continued to decrease with aggravation of the disease. High ratio cholesterol/ protein occurred in VLDLP and subfractions of LDLP from patient with severe NIDDM.


CONCLUSIONS
It is shown that subnormal resistance to oxidation of LDLP dense subfractions even in mild NIDDM is an informative diagnostic criterion of risk factor to develop atherosclerosis.
Ragino
Iu I
IuI
Dushkin
M I
MI
Nikitin
Iu P
IuP
D000328:Adult; D001161:Arteriosclerosis / Q000097:blood / Q000209:etiology; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000150:complications; D018450:Disease Progression; D005260:Female; D005470:Fluorometry; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008077:Lipoproteins, LDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D014461:Ultracentrifugation
D015415:Biomarkers; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D002784:Cholesterol