Detection of bluetongue virus genome segment 6 sequences by RT-PCR.
Reverse transcription-PCR (RT-PCR) technique was adopted to amplify a 101 basepair nucleotide sequence of bluetongue virus (BTV) genome segment 6. The specificity of the amplicon was determined by its approximate size in 3% agarose gel electrophoresis, digestion with restriction enzyme MspI, dot-blot hybridization and cycle sequencing. The technique was found to be suitable for detection of bluetongue virus in infected cell culture and clinical samples.
Bandyopadhyay
S K
SK
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India.
Kataria
R S
RS
Tiwari
A K
AK
D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics*; D002478:Cells, Cultured; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction