Voltammetric response and determination of DNA with a silver electrode.
A current from DNA was obtained using a silver electrode with low overpotentials for the first time. Experimental results revealed that the voltammetric response of DNA was attributed to the redox reactions of purine bases. It was also shown that such a method provided a convenient and practical way to determine DNA. A linear dependence of the peak currents on ssDNA concentrations was observed in the range 0.5-2.5 microg/mL. The relative standard deviation was 3.5% for six successive determinations at 0.5 microg/mL. The detection limit was 50 ng/mL. Influence of the structure and the length of the nucleic acids on their electrochemical behavior was discussed. In view of the merits of the silver electrode, this technique might provide new possibilities for further electrochemical research and determination of nucleic acids.
Fan
C
C
Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, People's Republic of China.
Song
H
H
Hu
X
X
Li
G
G
Zhu
J
J
Xu
X
X
Zhu
D
D
D000225:Adenine / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D003854:Deoxyribonucleotides / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D008297:Male; D009691:Nucleic Acid Denaturation; D010084:Oxidation-Reduction; D012474:Salmon; D012834:Silver
D003854:Deoxyribonucleotides; D012834:Silver; D004247:DNA; D000225:Adenine