title: High-performance liquid chromatographic determination of pholedrine in human serum using ion-pair extraction and amperometric detection.
abstract: The method for the quantitation of pholedrine in human serum involves extraction of the sample with benzene utilizing an ion-pairing reagent, bis(2-ethylhexyl)phosphoric acid, and re-extraction into diluted phosphoric acid. Analysis is carried out on a ODS reversed-phase column with heptanesulfonate as the ion-pairing reagent. The procedure allows quantitation at the lower nanogram level and is useful for pharmacokinetic investigations.
authors.affiliation: Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: G P
authors.initials: GP
authors.lastname: Peinhardt
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013566:Sympathomimetics / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D013566:Sympathomimetics; D008694:Methamphetamine; C005877:pholedrine