[The proliferative response and immunoglobulin production in peripheral blood lymphocytes stimulated with Japanese cedar pollen antigens].
Peripheral blood mononuclear cells taken from 38 normal subjects and 50 Japanese cedar pollinosis patients were cultured with or without mitogens or Japanese cedar pollen antigens to study the proliferative response and immunoglobulin production in periphral blood lymphocytes. When stimulated with Con A or anti-CD 40 mAb, the proliferative responses in the patient group were significantly higher than those in the normal group, and when stimulated with anti-CD 40 mAb, the IgG 2 production was markedly dominant among the IgE and IgG subclass productions in both groups. When stimulated with Japanese cedar pollen antigens, the proliferative responses in the patient group were markedly augmented in comparison with the normal group, and showed a correlation with the serum anti-cedar IgE antibody titers in the patient group. In regard to the immunoglobulin production in peripheral blood lymphocytes stimulated with Japanese cedar pollen antigens, the IgE productions in the patient group were significantly higher than those in the normal group, and showed a weak correlation with the serum anti-cedar IgE antibody titers in the patient group. The IgG productions were, however, slightly enhanced by the stimulation with Japanese cedar pollen antigens in both of the normal and patient groups. While it could not be clearly demonstrated which subclass of IgG was responsible for the enhancement of IgG production, there were some cases of relatively higher responses of IgG 2 or IgG 4 production. Though the IgG 4 productions in the patient group were significantly higher than those in normal group, there was no clear correlation with the serum anti-cedar IgE antibody titers in the patient group. On the other hand, it seems to be noteworthy that a weak negative correlation was recognized between the IgG 2 productions and the IgG 4 productions, and between the IgG 4 productions and the IgE productions. Considering these results in the present study, it was concluded to be difficult to identify a IgG 4 high responder in a course of clinical immunotherapy through the in vitro IgG 4 production or other related reactions as attempted in this study.
Hayashi
T
T
Division of Otorhinolaryngology, Takanobashi Chuoh Hospital, Hiroshima.
Hayashi
K
K
Jyo
T
T
Asaoku
R
R
Shigeta
S
S
Ono
K
K
Yamashita
U
U
D000328:Adult; D000941:Antigens / Q000276:immunology*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011058:Pollen; D014197:Trees
D000941:Antigens; D007136:Immunoglobulins