title: Aging accentuates and bone marrow transplantation ameliorates metabolic defects in Fabry disease mice.
abstract: Fabry disease is an X-linked metabolic disorder caused by a deficiency of alpha-galactosidase A (alpha-Gal A). The enzyme defect leads to the systemic accumulation of glycosphingolipids with alpha-galactosyl moieties consisting predominantly of globotriaosylceramide (Gb3). In patients with this disorder, glycolipid deposition in endothelial cells leads to renal failure and cardiac and cerebrovascular disease. Recently, we generated alpha-Gal A gene knockout mouse lines and described the phenotype of 10-week-old mice. In the present study, we characterize the progression of the disease with aging and explore the effects of bone marrow transplantation (BMT) on the phenotype. Histopathological analysis of alpha-Gal A -/0 mice revealed subclinical lesions in the Kupffer cells in the liver and macrophages in the skin with no gross lesions in the endothelial cells. Gb3 accumulation and pathological lesions in the affected organs increased with age. Treatment with BMT from the wild-type mice resulted in the clearance of accumulated Gb3 in the liver, spleen, and heart with concomitant elevation of alpha-Gal A activity. These findings suggest that BMT may have a potential role in the management of patients with Fabry disease.
authors.affiliation: Functional Genomics Unit, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: T
authors.initials: T
authors.lastname: Ohshima
authors.forename: R
authors.initials: R
authors.lastname: Schiffmann
authors.forename: G J
authors.initials: GJ
authors.lastname: Murray
authors.forename: J
authors.initials: J
authors.lastname: Kopp
authors.forename: J M
authors.initials: JM
authors.lastname: Quirk
authors.forename: S
authors.initials: S
authors.lastname: Stahl
authors.forename: C C
authors.initials: CC
authors.lastname: Chan
authors.forename: P
authors.initials: P
authors.lastname: Zerfas
authors.forename: J H
authors.initials: JH
authors.lastname: Tao-Cheng
authors.forename: J M
authors.initials: JM
authors.lastname: Ward
authors.forename: R O
authors.initials: RO
authors.lastname: Brady
authors.forename: A B
authors.initials: AB
authors.lastname: Kulkarni
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D016026:Bone Marrow Transplantation; D003433:Crosses, Genetic; D000795:Fabry Disease / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D005838:Genotype; D006028:Glycosphingolipids / Q000378:metabolism; D006321:Heart / Q000254:growth & development; D008099:Liver / Q000254:growth & development / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012867:Skin / Q000254:growth & development / Q000473:pathology / Q000648:ultrastructure; D000519:alpha-Galactosidase / Q000235:genetics
chemical_list: D006028:Glycosphingolipids; D000519:alpha-Galactosidase