title: Phagocyte-induced lipid peroxidation of different intravenous fat emulsions and counteractive effect of vitamin E.
abstract: Unsaturated fatty acids, a major component of fat emulsions used in parenteral nutrition, are prone to peroxidation which is an important feature of oxygen-associated tissue damage. We used the nitroblue tetrazolium (NBT) reduction test to measure the production of superoxide radicals by stimulated polymorphonuclear neutrophils (PMN) in the presence of different fat emulsions: Intralipid (containing 100% long-chain triacylglycerols, LCT), Vasolipid (a physical mixture of 50% LCT and 50% medium-chain triacylglycerols, MCT) and Structolipid (structured triacylglycerols containing 63% LCT and 37% MCT). We measured the amount of malonaldehyde (MDA) and 4-hydroxyalkenal to determine the lipid peroxidation of the three fat emulsions in the presence of stimulated neutrophils. Further, we investigated the role of vitamin E (alpha-tocopherol) in preventing lipid peroxidation in vitro. The results showed that the values of NBT reduction of PMN were significantly decreased in each of the three fat emulsions and that increasing concentrations of fat emulsions were associated with decreased values of NBT reductions, in a dose-dependent way (P<0.001). There were, however, no statistically significant differences between the values of the three different types of fat emulsions (P>0.05). Lipid peroxidation increased significantly in the presence of all three types of fat emulsions, and was more pronounced for Intralipid than for Vasolipid and Structolipid after 1 and 2 h of incubation with resting as well as with stimulated phagocytes. The increased lipid peroxidation of the fat emulsions was markedly reduced by vitamin E, and the inhibition was concentration dependent. In conclusion, lipid peroxidation in vitro is more pronounced when PMNs are incubated with fat emulsions. This increase in lipid peroxidation can be reduced by adding vitamin E to the fat emulsions.
authors.affiliation: Department of Clinical and Oral Bacteriology, Huddinge University Hospital, Karolinska Institute, Sweden.
authors.forename: G H
authors.initials: GH
authors.lastname: Wu
authors.forename: C
authors.initials: C
authors.lastname: Jarstrand
authors.forename: J
authors.initials: J
authors.lastname: Nordenström
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005217:Fat Emulsions, Intravenous / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000502:physiology*; D013481:Superoxides / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D005217:Fat Emulsions, Intravenous; D013481:Superoxides; D014810:Vitamin E; D009580:Nitroblue Tetrazolium; D008315:Malondialdehyde