Mapping of the antigenic regions of streptokinase in humans after streptokinase therapy.
Streptokinase (SK) is efficaciously used as a thrombolytic drug for the treatment of myocardial infarction. Being a bacterial protein, SK is immunogenic in humans. Therefore, resulting from SK therapy, patients become immunized and anti-SK antibody (Ab) titers rise post-treatment. High Ab titers might provoke severe immune reactions during SK therapy and neutralize SK activity, preventing effective thrombolysis. Spot synthesis combined with peptide library techniques is a useful tool for studying protein-peptide interactions on continuous cellulose membranes. Here, we report on the mapping of antigenic regions of SK using a spot-synthesized peptide library and human total sera from patients receiving SK therapy. All tested samples have high anti-SK Ab titers and most of them show significant SK neutralizing capacity. Individual variations in peptide recognition were detected. However, patients treated with SK tend, in general, to show a common regional binding pattern, including residues 1-20, 130-149, 170-189, and 390-399. This is the first study reporting the probing of a cellulose-bound set of peptides with total human sera.
Torréns
I
I
Division of Pharmaceutical, Centro de Ingeniería Genética y Biotecnología, Havana, Cuba. Isis@cigb.edu.cu
Reyes
O
O
Ojalvo
A G
AG
Seralena
A
A
Chinea
G
G
Cruz
L J
LJ
de la Fuente
J
J
D000595:Amino Acid Sequence; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000276:immunology; D003462:Cuba; D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000097:blood* / Q000628:therapy; D010446:Peptide Fragments / Q000276:immunology; D019151:Peptide Library; D011485:Protein Binding / Q000276:immunology; D012699:Serology; D013300:Streptokinase / Q000276:immunology* / Q000627:therapeutic use
D001426:Bacterial Proteins; D010446:Peptide Fragments; D019151:Peptide Library; D013300:Streptokinase