Cloning, sequencing and characterisation of a downstream region of ksdDI operon of Arthrobacter simplex.
An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.
Dziadek
J
J
Centre for Microbiology and Virology, Polish Academy of Sciences, Lódź, Poland.
Yamashita
M
M
Murooka
Y
Y
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013251:Steroid Isomerases / Q000737:chemistry / Q000235:genetics*; D013256:Steroids / Q000378:metabolism
D013256:Steroids; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase; D013251:Steroid Isomerases; C022660:steroid delta-isomerase