Genotoxicity of water samples from Dianchi lake detected by the Vicia faba micronucleus test.
Dianchi Lake covers about a 300 km2 area. Kunming city on the edge of the lake is surrounded by industrial establishments. Farm land surrounds the remaining areas of the lake. The lake water is polluted by the Kunming city municipal sewage from 3 million inhabitants, the industrial effluent and farm runoff. Water samples were collected from 12 sites along the shore of the lake during the dry (May) and rainy (August) seasons for genotoxicity testing with the Vicia micronucleus assay during the year 1995. Genotoxicity in terms of micronuclei (MCN) frequencies in the root tip cells of Vicia showed a consistent elevated frequency of MCN over the control sample at the 0.05-0.01 levels of significance in both seasons. The MCN frequencies of sites A (Daguanhe), B (Gaoqiao) and C (Xiyuan) were 3.5-4 times as high as the control values (5.25/1000 cells) in the dry season. In the rainy season, the MCN frequencies of water samples from most of the 12 sites were relatively lower than those of the dry season except sites J (Haikou), and K (Kunyang). The average MCN frequency of the dry season samples was 14.97 per 1000 cells and that of the rainy season samples was 12.24 per 1000 cells while the average control value was around 5.00/1000 cells.
Duan
C Q
CQ
Biology Department, Yunnan University, Kunming, 650091, China.
Hu
B
B
Jiang
X H
XH
Wen
C H
CH
Wang
Z
Z
Wang
Y X
YX
D001681:Biological Assay / Q000379:methods; D002681:China; D004784:Environmental Monitoring / Q000379:methods*; D007887:Fabaceae; D005618:Fresh Water; D005843:Geography; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D018517:Plant Roots; D010946:Plants, Medicinal; D012722:Sewage; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014881:Water Supply
D009153:Mutagens; D012722:Sewage; D014874:Water Pollutants, Chemical