Reversal of impaired calcium homeostasis in the rat diaphragm subjected to Monocrotaline-induced pulmonary hypertension.
Monocrotaline (MCT) pneumotoxicity is known to alter the structure of pulmonary vascular wall and impairs endothelial cell function resulting in pulmonary hypertension. Its effect on the diaphragm muscle has not yet been elucidated. This study examines the effect of MCT pneumotoxicity on calcium transport in the rat diaphragm. Pulmonary hypertension induced by MCT pneumotoxicity caused a significant increase (P < 0.001) in calcium accumulation in strips isolated from rat diaphragms. Treatment of rats having received MCT with Indapamide reduced calcium uptake by diaphragmatic strips to levels that are not significantly different from the control (P > 0.05). Treatment with Indapamide alone did not elicit any change in calcium accumulation in the diaphragmatic strips. Treatment of the animals with MCT, Indapamide or both did not produce any significant change (P > 0.05) in the cell volume of the diaphragmatic strips. Pulmonary hypertension increased calcium uptake by the muscle cells in the rat diaphragm which may alter diaphragmatic contractility; an effect that was prevented by Indapamide.
Kanj
N A
NA
Department of Internal Medicine, American University of Beirut Medical Center, Lebanon.
Nasser
M G
MG
Medawar
W A
WA
Al Tayeh
A U
AU
Khoury
M Y
MY
Nassar
C F
CF
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000493:pharmacokinetics; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002273:Carcinogens / Q000009:adverse effects*; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D006976:Hypertension, Pulmonary / Q000139:chemically induced / Q000503:physiopathology* / Q000517:prevention & control; D066298:In Vitro Techniques; D007190:Indapamide / Q000494:pharmacology; D008297:Male; D016686:Monocrotaline / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D002273:Carcinogens; D016686:Monocrotaline; D007190:Indapamide; D002118:Calcium