Identification and characterization of G90, a novel mouse RNA that lacks an extensive open reading frame.
We describe the cloning and characterization of the murine G90 gene, identified by subtractive hybridization based on the differential presence of its transcript in large and small intestine. The full-length cDNA and genomic sequences were cloned and found to produce a 1.5kb transcript that is polyadenylated but has no open reading frame larger than 249bp. The G90 gene was mapped to the proximal region of mouse chromosome 6. Expression analysis by Northern blotting showed that G90 is transcribed at very high levels in the small intestine and at lower levels in large intestine, testis and kidney of the mouse. In situ hybridization analysis on sections of small and large intestine and testis showed that G90 transcripts are present only in post-mitotic cells.
Krause
R
R
Institut für Biologie III der Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany.
Hemberger
M
M
Himmelbauer
H
H
Kalscheuer
V
V
Fundele
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D007420:Intestine, Large / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D011061:Poly A; D012313:RNA