Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool.
A simple method for the gas-liquid chromatographic quantitation of human fecal bile acids and sterols is described where bile acids are subjected to n-butyl ester derivatization, without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile acids. Under these conditions, bile acid derivatives are well resolved from each other and from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method was shown to be highly reproducible and recoveries were similar to those obtained with other methods used for fecal bile acid analysis. Application of the method for bile acid and sterol analysis in human stool is described.
Batta
A K
AK
Department of Medicine, Gastroenterology Division, University of Medicine and Dentistry-New Jersey Medical School, Newark, NJ, USA.
Salen
G
G
Rapole
K R
KR
Batta
M
M
Batta
P
P
Alberts
D
D
Earnest
D
D
D001647:Bile Acids and Salts / Q000032:analysis*; D004083:Cholestanol / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D003840:Deoxycholic Acid / Q000032:analysis; D005243:Feces / Q000737:chemistry*; D006801:Humans; D008095:Lithocholic Acid / Q000032:analysis; D015203:Reproducibility of Results; D013261:Sterols / Q000032:analysis*; D014297:Trimethylsilyl Compounds
D001647:Bile Acids and Salts; D013261:Sterols; D014297:Trimethylsilyl Compounds; D003840:Deoxycholic Acid; D008095:Lithocholic Acid; D004083:Cholestanol; D002784:Cholesterol