title: Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR.
abstract: The presence of the ileS-2 gene, responsible for mupirocin resistance, in clinical isolates of methicillin-resistant Staphylococcus aureus was determined by multiplex polymerase chain reaction. Three pairs of primers were used, which yielded specific fragments of femA (encoding a unique feature of S. aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The multiplex polymerase chain reaction system is an easy and time-saving technique that, together with a rapid method for DNA extraction by boiling, may be incorporated as a routine analysis in clinical diagnostic laboratories.
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: E L
authors.initials: EL
authors.lastname: Nunes
authors.forename: K R
authors.initials: KR
authors.lastname: dos Santos
authors.forename: P J
authors.initials: PJ
authors.lastname: Mondino
authors.forename: M do C
authors.initials: Mdo C
authors.lastname: Bastos
authors.forename: M
authors.initials: M
authors.lastname: Giambiagi-deMarval
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016712:Mupirocin / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017931:DNA Primers; C096686:FemA protein, Bacteria; D016712:Mupirocin