title: Precise quantitation of human parvovirus B19 DNA in biological samples by PCR.
abstract: An application of a quantitative PCR-based method was developed for the detection of human parvovirus B19 DNA. The procedure was characterised according to guidelines for the validation of analytical procedures. Furthermore, the reliability was demonstrated by the correct quantitation of samples of an international collaborative study. This application might be useful for studies focussed on removal and/or inactivation procedures of human parvovirus B19 as well as for general screening purposes of biological materials.
authors.affiliation: Baxter-IMMUNO, Biomedical Research Center, Orth/Donau, Austria.
authors.forename: F
authors.initials: F
authors.lastname: Gruber
authors.forename: F G
authors.initials: FG
authors.lastname: Falkner
authors.forename: F
authors.initials: F
authors.lastname: Dorner
authors.forename: T
authors.initials: T
authors.lastname: Hämmerle
mesh_terms: D001483:Base Sequence; D001688:Biological Products / Q000009:adverse effects / Q000032:analysis* / Q000592:standards; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D004340:Drug Contamination; D005069:Evaluation Studies as Topic; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D015203:Reproducibility of Results
chemical_list: D001688:Biological Products; D017931:DNA Primers; D004279:DNA, Viral