title: [Cloning the 5 ' end fragment of ST13 cDNA by nested PCR].
abstract: OBJECTIVE
  To clone and sequence the 5' -end fragment of ST13 cDNA.
  
  
  METHODS
  The 5' -end fragment of the ST13cDNA was amplified directly from cDNA library by nested PCR method, and cloned into pGEM-T. easy vector, then the sequencing of the inserted PCR product was performed.
  
  
  RESULTS
  After primary and secondary PCR, two PCR products obtained with the size of about 550bp and 480bp, respectively, were both proved to be the 5' -end of ST13 cDNA by sequencing.
  
  
  CONCLUSIONS
  the method presented is very simple and effective for cloning and sequencing the 5'-end of a target gene.
authors.affiliation: Cancer Institute of Zhejiang Medical University, Hangzhou, 310009 P.R. China. jcao@public.hz.zj.cn
authors.forename: L
authors.initials: L
authors.lastname: Geng
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: Y
authors.initials: Y
authors.lastname: Fang
mesh_terms: D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*