title: Characterization and localization of beta2-adrenergic receptors in the bovine oviduct: indication for progesterone-mediated expression.
abstract: Beta2-adrenergic receptors were detected in bovine oviductal epithelium by use of receptor binding studies and expression analysis. Complementary DNA cloning gave use to the first full-length bovine beta2-adrenoceptor messenger RNA sequence (2030 bases). Receptor bioactivity in oviduct epithelial cells was characterized by specific ligand interaction and consequent cAMP generation. Expression studies demonstrated an estrous cycle-dependent regulation, with higher transcript levels and significantly increased binding capacity during the luteal phase. After progesterone supplementation, oviduct epithelial cells showed elevated receptor expression in culture, supporting the hypothesis that progesterone up-regulates the beta2-adrenergic receptor within these cells. It seems likely that catecholamines from the circulation or from innervation might be able to influence reproductive success by regulating oviductal secretion.
authors.affiliation: Institute of Physiology, Technical University Munich-Weihenstephan, Freising, Germany. einspani@weihenstephan.de
authors.forename: R
authors.initials: R
authors.lastname: Einspanier
authors.forename: C
authors.initials: C
authors.lastname: Gabler
authors.forename: A
authors.initials: A
authors.lastname: Kettler
authors.forename: W
authors.initials: W
authors.lastname: Kloas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004837:Epinephrine / Q000494:pharmacology; D005187:Fallopian Tubes / Q000737:chemistry*; D005260:Female; D008969:Molecular Sequence Data; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D018343:Receptors, Adrenergic, beta-2 / Q000032:analysis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D011374:Progesterone; D004837:Epinephrine