[Study of the antigenic structure of tick-borne encephalitis virus using synthetic peptides].
A number of peptides, fragments of the envelope protein E of the tick-borne encephalitis virus (Sofjin strain), were synthesized. Their binding to the polyclonal antiserum to protein E was studied. Rats were immunized with both the free peptides and their KLH-conjugates, and the resulting antisera were tested for their reactivity toward protein E and for their neutralizing activity toward the virus in cell culture. The only peptide corresponding to the 98-113 sequence of protein E was shown to be bound by the protein E antiserum in EIA. Two-fold immunization of rats with KLH-conjugates of the peptides corresponding to the 98-113, 130-143, and 394-403 sequences of protein E resulted in antipeptide antibodies capable of binding the native protein E, and the antibodies to the 98-113 and 394-403 peptides were capable of neutralizing the virus.
Volkova
T D
TD
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Vol'pina
O M
OM
Ivanov
V T
VT
Rubin
S G
SG
Semashko
I V
IV
Karavanov
A S
AS
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D006433:Hemocyanins / Q000737:chemistry; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000737:chemistry*; D051381:Rats; D013552:Swine; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology
D000956:Antigens, Viral; D015335:Molecular Probes; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin