title: Phosphoglycosylation of a secreted acid phosphatase from Leishmania donovani.
abstract: The secreted acid phosphatase (SAcP) of L.donovani is a heterogeneous glycoprotein that displays a wide array of N- and O-linked glycosylations. The O-linked sugars are of particular interest due to their similarity to the phosphoglycan structures of the major lipophosphoglycan surface antigen and released phosphoglycan (Turco et al., 1987; Greis et al., 1992). This study describes a structural analysis of the SAcP O-linked glycosylations using mass spectroscopy, amino acid sequencing, and enzymatic carbohydrate sequencing. Analysis of glycan chain lengths and peptide glycosylation site distribution was performed, revealing that the average O-linked structure was approximately 32 repeat units in length. Amino acid sequence analysis of glycosylated peptides showed that phosphoglycosylations did not occur randomly but were localized to specific serine residues within an array of degenerate serine/threonine-rich repeat sequences localized in the C-terminus. No evidence was obtained for modification of threonine residues. The observed pattern suggested that a consensus sequence may exist for localization of phosphoglycan structures.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 2Y2, Canada.
authors.forename: D N
authors.initials: DN
authors.lastname: Lippert
authors.forename: D W
authors.initials: DW
authors.lastname: Dwyer
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: R W
authors.initials: RW
authors.lastname: Olafson
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D006031:Glycosylation; D006834:Hydrazines / Q000378:metabolism; D007893:Leishmania donovani / Q000201:enzymology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D006834:Hydrazines; D010446:Peptide Fragments; D011134:Polysaccharides; C029424:hydrazine; D000135:Acid Phosphatase