Purification of bovine S100A12 from recombinant Escherichia coli.
S100A12, a member of the S100 family of EF-hand calcium-binding proteins, was purified from Escherichia coli cells expressing the corresponding cDNA. The procedure involved washing induced E. coli cells with EDTA-containing hypotonic solution, ion-exchange chromatography, and HPLC. Recombinant S100A12 was purified to homogeneity with the final yield around 6.7 mg per 20 ml of culture. The purified protein was identical to native S100A12 in the N-terminal amino acid sequence, lysylendopeptidase peptide mapping, mass spectrum, and Ca2+-dependent binding affinity to amlexanox, an antiallergy drug. However, the N-terminal methionine residue of the purified protein was not cleaved off as in the native protein. The method used in the present study permits the purification of recombinant S100A12 in large quantities and may also be applicable to preparation of other S100 family proteins.
Yamashita
K
K
Department of Chemistry, Kagawa Medical University, Kagawa, 761-0793, Japan.
Oyama
Y
Y
Shishibori
T
T
Matsushita
O
O
Okabe
A
A
Kobayashi
R
R
D000595:Amino Acid Sequence; D000631:Aminopyridines / Q000378:metabolism; D000818:Animals; D018926:Anti-Allergic Agents / Q000378:metabolism; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D066298:In Vitro Techniques; D008168:Lung / Q000737:chemistry; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009418:S100 Proteins; D000067616:S100A12 Protein
D000631:Aminopyridines; D018926:Anti-Allergic Agents; D002135:Calcium-Binding Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human; C045742:amlexanox