title: Antimutagenic activity of chemical fractions isolated from a commercial soybean processing by-product.
abstract: Commercial products of agronomic crop plants may become a reliable and inexpensive source of phytonutrients, such as antimutagenic food supplements. We previously demonstrated that PCC, an ethanol extract of a commercial soybean processing by-product, was able to repress induced genomic DNA damage, whole cell clastogenicity, and point mutation in mammalian cells. In this paper we separated PCC into a series of chemically defined fractions and determined their ability to repress induced mutagenic damage in Chinese hamster lung cells, Chinese hamster ovary cells and human lymphocytes. Fraction PCC70 (PCC 70% methanol eluate) contained the flavonoids from PCC and daidzin and genistin repressed 2-acetoxyacetylaminofluorene (2AAAF)-induced DNA damage measured with single cell gel electrophoresis. Genistein, however, enhanced the genotoxic impact of 2AAAF. Fraction PCC100 (PCC 100% methanol eluate) had the greatest level of antigenotoxic activity against 2AAAF in CHO cells and repressed the genotoxic capacity of the dietary carcinogen 2-amino-3-methylimidazo-(4,5-f)quinoline (IQ) in human lymphocytes. These data indicate that commercial soybean products and by-products may be a source of chemoprotective food additives.
authors.affiliation: Department of Crop Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign, USA. m-plewa@uiuc.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Plewa
authors.forename: E D
authors.initials: ED
authors.lastname: Wagner
authors.forename: M A
authors.initials: MA
authors.lastname: Berhow
authors.forename: A
authors.initials: A
authors.lastname: Conway
authors.forename: A L
authors.initials: AL
authors.lastname: Rayburn
authors.forename: D
authors.initials: D
authors.lastname: Anderson
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D002458:Cell Fractionation; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005511:Food Handling; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009152:Mutagenicity Tests; D013025:Glycine max / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016587:Antimutagenic Agents