title: Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle.
abstract: The enzymic regulation of triacylglycerol breakdown in skeletal muscle is poorly understood. Western blotting of muscle fibres isolated by collagenase treatment or after freeze-drying demonstrated the presence of immunoreactive hormone-sensitive lipase (HSL), with the concentrations in soleus and diaphragm being more than four times the concentrations in extensor digitorum longus and epitrochlearis muscles. Neutral lipase activity determined under conditions optimal for HSL varied directly with immunoreactivity. Expressed relative to triacylglycerol content, neutral lipase activity in soleus muscle was about 10 times that in epididymal adipose tissue. In incubated soleus muscle, both neutral lipase activity against triacylglycerol (but not against a diacylglycerol analogue) and glycogen phosphorylase activity increased in response to adrenaline (epinephrine). The lipase activation was completely inhibited by anti-HSL antibody and by propranolol. The effect of adrenaline could be mimicked by incubation of crude supernatant from control muscle with the catalytic subunit of cAMP-dependent protein kinase, while no effect of the kinase subunit was seen with supernatant from adrenaline-treated muscle. The results indicate that HSL is present in skeletal muscle and is stimulated by adrenaline via beta-adrenergic activation of cAMP-dependent protein kinase. The concentration of HSL is higher in oxidative than in glycolytic muscle, and the enzyme is activated in parallel with glycogen phosphorylase.
authors.affiliation: Department of Applied Physiology, The Polish Academy of Sciences, Warsaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Langfort
authors.forename: T
authors.initials: T
authors.lastname: Ploug
authors.forename: J
authors.initials: J
authors.lastname: Ihlemann
authors.forename: M
authors.initials: M
authors.lastname: Saldo
authors.forename: C
authors.initials: C
authors.lastname: Holm
authors.forename: H
authors.initials: H
authors.lastname: Galbo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002384:Catalysis; D004789:Enzyme Activation; D004837:Epinephrine / Q000494:pharmacology*; D006651:Histocytochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: D002787:Sterol Esterase; D004837:Epinephrine