Construction and characterization of a sheep BAC library of three genome equivalents.
A sheep BAC library of over three genome equivalents was constructed and arrayed in superpools and row, column, and plate pools. The library contains 90,000 clones distributed in 39 superpools. The average insert size was estimated at 123 kb. The library was screened by PCR with 77 primer pairs corresponding to ovine microsatellites distributed throughout the genome. The probability of finding a random sequence in the library could be estimated at 0.96.
Vaiman
D
D
Laboratoire de Génétique biochimique et de Cytogénétique, Département de Génétique Animale, INRA, 78352 Jouy-en-Josas, France.
Billault
A
A
Tabet-Aoul
K
K
Schibler
L
L
Vilette
D
D
Oustry-Vaiman
A
A
Soravito
C
C
Cribiu
E P
EP
D000818:Animals; D002876:Chromosomes, Bacterial; D015247:Deoxyribonuclease HindIII / Q000235:genetics; D015723:Gene Library; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018895:Microsatellite Repeats; D011328:Prions / Q000235:genetics; D012756:Sheep / Q000235:genetics*; D014960:X Chromosome
D011328:Prions; D015247:Deoxyribonuclease HindIII