title: [Resistance in a strain of Culex quinquefasciatus coming from Medellín, Colombia].
abstract: The levels of susceptibility and/or resistance to 5 organophosphate insecticides (malathion, methyl-pyrimifos, clorpirifos, temephos and fenthion), 4 pyrethroids (cypermethrin, deltamethrin, permethrin and lambda-cyhalothrin), and a carbamate (propoxur) were determined in order to know the state of resistance in a strain of Culex quinquefasciatus from a locality of the city of Medellín, Colombia. Resistance to all organophosphate insecticides, though with relatively lower values for methyloirimifos and fenthion, was observed. No resistance to lambda-cyhalothrin and cypermethrin or to propoxur was found. These insecticides may be useful for the control of mosquitoes in Colombia. It was demonstrated by using the piperonil butoxide synergist that the oxidases of multiple function played an important role in the resistance to organophosphate insecticides and pyrethroids. The utilization of S.S.S. tributyl phosphotritiate revealed that the superproduction of unspecific esterases was a mechanism of resistance to organophosphate insecticides, except methyl-pirimifos and for perythroids, except lambda-cyhalothrin. This result should be taken into consideration for the strategies to be used to control Culex quinquefasciatus in Colombia. These two mechanism of resistance are not responsible for the resistance to propoxur. The electrophoretic analysis showed the presence of esterases B1, A6 and B6, which seem to have an important function in resistance.
authors.affiliation: Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
authors.forename: J
authors.initials: J
authors.lastname: Bisset
authors.forename: M M
authors.initials: MM
authors.lastname: Rodríguez
authors.forename: C
authors.initials: C
authors.lastname: Díaz
authors.forename: A
authors.initials: A
authors.lastname: Soca
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods / Q000706:statistics & numerical data; D002140:California; D003105:Colombia; D003465:Culex / Q000201:enzymology / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis / Q000235:genetics; D005838:Genotype; D007305:Insecticide Resistance / Q000235:genetics
chemical_list: D004950:Esterases