Detection of a novel DNA virus (TTV) sequence in peripheral blood mononuclear cells.
DNA sequences of a novel DNA virus (TTV) were examined in 81 peripheral blood mononuclear cell (PBMC) DNA samples from 48 children and 33 adults, 22 cord blood mononuclear cells (CBMC) DNA samples, and 7 autopsy liver tissue DNA samples by a hemi-nested polymerase chain reaction (PCR). The PCR was carried out using the published primers (NG059, NG061, NG063) to amplify TTV DNA sequences. The sequences were detected in 4 of 81 (5%) PBMC DNA samples, in none of 22 (0%) CBMC DNA samples, and in 2 of 7 (29%) liver tissue DNA samples by direct gel analysis. The PCR-amplified products were confirmed by direct sequencing. The sequencing showed considerable diversities, with differences of 0-55% in 6 TTV isolates, compared with the prototype sequence of TTV. These results suggest that TTV is a ubiquitous virus that produces asymptomatic infection in a large proportion of the general population without transfusion of blood-derived products. To our knowledge, this is the first report describing the detection of TTV DNA sequences in PBMCs.
Okamura
A
A
Department of Pediatrics, Hokkaido University School of Medicine, Sapporo, Japan.
Yoshioka
M
M
Kubota
M
M
Kikuta
H
H
Ishiko
H
H
Kobayashi
K
K
D000328:Adult; D001344:Autopsy; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004266:DNA Virus Infections / Q000821:virology*; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000821:virology; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008099:Liver / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D017422:Sequence Analysis, DNA
D004279:DNA, Viral