Rapid identification of Debaryomyces hansenii/Candida famata by polymerase chain reaction.
Primers designed in this study were used in a polymerase chain reaction test to amplify a species-specific fragment of approximately 340 bp of the large subunit ribosomal DNA of Debaryomyces hansenii/Candida famata. None of the other medically relevant yeasts including C. guilliermondii, and also the related species, D. nepalensis and C. saitoana, were amplified by this primer pair.
Nishikawa
A
A
Department of Immunobiology, Meiji Pharmaceutical University, Tokyo, Japan.
Sugita
T
T
Shinoda
T
T
D002175:Candida / Q000145:classification* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D009181:Mycoses / Q000382:microbiology; D016133:Polymerase Chain Reaction; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity; D015003:Yeasts / Q000145:classification / Q000235:genetics
D004271:DNA, Fungal; D004275:DNA, Ribosomal