Method for measuring in vivo oxygen transport rates in a bioartificial organ.
Oxygen transport is crucial for the proper functioning of a bioartificial organ. In many cases, the immunoisolation membrane used to protect the transplanted cells from the host's immune system can be a significant barrier to oxygen transport. A method is described for measuring the in vitro and in vivo oxygen transport characteristics of a planar immunoisolation membrane. The in vitro oxygen permeability of the membrane was found to equal 9.22 x 10(-4) cm/sec and was essentially the same as the in vivo value of 9.51 x 10(-4) cm/sec. The fact that the in vitro and in vivo membrane permeabilities are identical indicates that any fibrotic tissue adjacent to the immunoisolation membrane did not present a significant resistance to the transport of oxygen. The measured oxygen permeability was also found consistent with the solute permeabilities obtained in a previous study for larger molecules. Based on the oxygen permeability results, theoretical calculations for this particular membrane indicate that about 1,100 islets of Langerhans/cm2 of membrane area can be sustained at high tissue densities and only 660 islets/cm2 can be supported at low tissue densities.
Whalen
D W
DW
Department of Bioengineering, University of Toledo, Toledo, Ohio 43606, USA.
Ding
Z
Z
Fournier
R L
RL
D000818:Animals; D001187:Artificial Organs; D002462:Cell Membrane / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D004285:Dogs; D000882:Haplorhini; D007515:Islets of Langerhans / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D008567:Membranes, Artificial; D008954:Models, Biological; D010100:Oxygen / Q000097:blood / Q000378:metabolism; D010539:Permeability
D008567:Membranes, Artificial; D010100:Oxygen