Excretion study of the beta2-agonist reproterol in human urine.
An excretion study of the beta2-agonist 7-[3-[(beta-3,5-trihydroxyphenethyl)amino]-propyl]theophylline (reproterol) in human urine, which is reportedly misused by athletes and horses as a doping agent, is presented. The study was performed after an oral administration of 20 mg of reproterol hydrochloride. The collected urine samples were prepared using the standard anabolic steroid extraction procedure and analyzed by gas chromatography coupled with quadrupole mass spectrometry and, also, with high-resolution mass spectrometry (HRMS). The main reproterol metabolite was found, whereas unchanged reproterol was not detected. The structure of the main metabolite was confirmed by an accurate HRMS measurement of diagnostic ions. Finally, an excretion urine profile of the main metabolite is presented. The mass spectrum of another possible unidentified reproterol metabolite is also reported.
Georgakopoulos
C G
CG
Doping Control Laboratory of Athens, Olympic Athletic Center of Athens, Maroussi, Greece. oaka@compulink.gr
Tsitsimpikou
C
C
Spyridaki
M H
MH
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000652:urine*; D004300:Doping in Sports; D004338:Drug Combinations; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009921:Metaproterenol / Q000031:analogs & derivatives* / Q000652:urine; D013806:Theophylline / Q000031:analogs & derivatives* / Q000652:urine
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D004338:Drug Combinations; C014792:reproterol; D009921:Metaproterenol; D013806:Theophylline