title: Molecular and genetic damage from environmental tobacco smoke in young children.
abstract: To assess the risks of early life exposure to environmental tobacco smoke (ETS), we tested whether four biomarkers in peripheral blood were associated with home ETS exposure in Hispanic and African-American children. The biomarkers included cotinine (a metabolite of nicotine) and three indicators of molecular and genetic damage from mutagens/carcinogens, protein adducts formed by the carcinogens 4-aminobiphenyl (4-ABP) and polycyclic aromatic hydrocarbons (PAHs), and sister chromatid exchanges (SCEs). We also explored possible ethnic differences in biomarkers. The study cohort comprised 109 Hispanic and African-American preschool children (1-6 years of age). Plasma cotinine was analyzed by gas chromatography, 4-ABP-hemoglobin adducts by gas chromatography-mass spectroscopy, PAH-albumin adducts by ELISA, and SCEs by cytogenetic techniques. Data on the amount of smoking by mothers (average 10.5 cigarettes per day) and other household members and regular visitors (average 6.5 cigarettes per day) were obtained by interview-administered questionnaires. Cotinine, 4-ABP-hemoglobin adducts, and PAH-albumin were significantly higher (P < 0.05) in the ETS-exposed children compared with the unexposed. SCEs were marginally higher (P = 0.076). African-American children had higher levels of cotinine (P = 0.059) and PAH-albumin (P = 0.02) than Hispanic children, after controlling for exposure to ETS. These results indicate molecular and genetic damage in minority children with
authors.affiliation: Division of Environmental Health Sciences, Columbia School of Public Health, New York, New York 10032, USA.
authors.forename: D
authors.initials: D
authors.lastname: Tang
authors.forename: D
authors.initials: D
authors.lastname: Warburton
authors.forename: S R
authors.initials: SR
authors.lastname: Tannenbaum
authors.forename: P
authors.initials: P
authors.lastname: Skipper
authors.forename: R M
authors.initials: RM
authors.lastname: Santella
authors.forename: G S
authors.initials: GS
authors.lastname: Cereijido
authors.forename: F G
authors.initials: FG
authors.lastname: Crawford
authors.forename: F P
authors.initials: FP
authors.lastname: Perera
mesh_terms: D000611:Aminobiphenyl Compounds / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D044383:Black People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003367:Cotinine / Q000097:blood; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D009369:Neoplasms / Q000208:ethnology* / Q000209:etiology*; D009519:New York City / Q000453:epidemiology; D011084:Polycyclic Aromatic Hydrocarbons / Q000097:blood; D012854:Sister Chromatid Exchange; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D044465:White People / Q000235:genetics; D001741:Black or African American
chemical_list: D000611:Aminobiphenyl Compounds; D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D014028:Tobacco Smoke Pollution; C006757:4-biphenylamine; D003367:Cotinine