Naturally occurring Tyzzer's disease in a calf.
Naturally occurring Clostridium piliforme infection (Tyzzer's disease) was found in a calf. Light microscopic examination revealed multifocal coagulative necrosis in the liver, catarrhal gastroenteritis, tracheitis and pneumonia, and thymic atrophy. Warthin-Starry staining clearly showed large filamentous bacilli in bundles or criss-cross patterns within the hepatocytes and epithelium and smooth muscle cells of the ileum and cecum. Immunohistochemistry using anti-C. piliforme RT and MSK strain antisera showed positive reaction against the bacilli. Electron microscopic examination revealed bacilli within the hepatocytes that demonstrated a characteristic vegetative form, with peritrichous flagella, and spores. The polymerase chain reaction (PCR) study using the paraffin-embedded liver sections, the 196-bp DNA fragment specific to 16S ribosomal RNA of C. piliforme was amplified. The characteristics of these bacilli are consistent with those of of C. piliforme. The PCR technique using paraffin-embedded sections should be useful for confirming C. piliforme infection in spontaneous cases.
Ikegami
T
T
Shirota
K
K
Une
Y
Y
Nomura
Y
Y
Wada
Y
Y
Goto
K
K
Takakura
A
A
Itoh
T
T
Fujiwara
K
K
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D003013:Clostridium / Q000472:pathogenicity*; D003015:Clostridium Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D017809:Fatal Outcome; D007150:Immunohistochemistry; D007564:Japan; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S