In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica.
The activity of asiaticoside, isolated from Centella asiatica, has been studied in normal as well as delayed-type wound healing. In guinea pig punch wounds topical applications of 0.2% solution of asiaticoside produced 56% increase in hydroxyproline, 57% increase in tensile strength, increased collagen content and better epithelisation. In streptozotocin diabetic rats, where healing is delayed, topical application of 0.4% solution of asiaticoside over punch wounds increased hydroxyproline content, tensile strength, collagen content and epithelisation thereby facilitating the healing. Asiaticoside was active by the oral route also at 1 mg/kg dose in the guinea pig punch wound model. It promoted angiogenesis in the chick chorioallantoic membrane model at 40 microg/disk concentration. These results indicate that asiaticoside exhibits significant wound healing activity in normal as well as delayed healing models and is the main active constituent of Centella asiatica.
Shukla
A
A
Pharmacology Division, Central Drug Research Institute, Lucknow, India. gshukla@zoo.uvm.edu1
Rasik
A M
AM
Jain
G K
GK
Shankar
R
R
Kulshrestha
D K
DK
Dhawan
B N
BN
D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D002642:Chick Embryo; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008954:Models, Biological; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000494:pharmacology; D013997:Time Factors; D014315:Triterpenes / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
D000890:Anti-Infective Agents; D010936:Plant Extracts; D014315:Triterpenes; D013311:Streptozocin; C004446:asiaticoside