title: Effect of toxin-g from Tityus serrulatus scorpion venom on gastric emptying in rats.
abstract: The effect of toxin-gamma from Tityus serrulatus scorpion venom on the gastric emptying of liquids was studied in 176 young adult male Wistar rats (2-3 months of age) divided into subgroups of 8 animals each. Toxin-gamma was injected i.v. at doses of 25, 37.5, 50 or 100 micrograms/kg and the effect on gastric emptying was assessed 30 min and 8 h later. A time-course study was also performed by injecting 50 micrograms of toxin-gamma/kg and measuring the effect on gastric emptying at times 0.25, 0.5, 1, 2, 4, 8, 24 and 48 h post-venom. Each envenomed animal was paired with its saline control and all received a saline test meal solution containing phenol red (60 micrograms/ml) as a marker. Ten minutes after administering the test meal by gavage the animals were sacrificed and gastric retention was determined by measuring the residual marker concentration of the test meal. A significant delay in gastric emptying, at 30 min and 8 h post-venom, was observed only after 50 and 100 micrograms of toxin-gamma/kg compared to control values. The responses to these two doses were significantly different after 8 h post-venom. Toxin-gamma (50 micrograms/kg) significantly delayed the gastric emptying of liquids at all times studied, with a peak response at 4 h after toxin administration compared to control values. These results indicate that the i.v. injection of toxin-gamma may induce a rapid, intense and sustained inhibition of gastric emptying 0.25 to 48 h after envenomation.
authors.affiliation: Departamento de Pediatria, Universidade Estadual de Campinas, SP, Brasil. bucaret@turing.unicamp.br
authors.forename: F
authors.initials: F
authors.lastname: Bucaretchi
authors.forename: A M
authors.initials: AM
authors.lastname: Vinagre
authors.forename: C
authors.initials: C
authors.lastname: Chavéz-Olórtegui
authors.forename: E F
authors.initials: EF
authors.lastname: Collares
mesh_terms: D000818:Animals; D005746:Gastric Emptying / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000633:toxicity*
chemical_list: D012604:Scorpion Venoms