Interstrain variation in the human cytomegalovirus DNA polymerase sequence and its effect on genotypic diagnosis of antiviral drug resistance. Adult AIDS Clinical Trials Group CMV Laboratories.
The polymerase (pol) coding sequence was determined for 40 independent clinical cytomegalovirus isolates sensitive to ganciclovir and foscarnet. Sequence alignments showed >98% interstrain homology and amino acid variation in only 4% of the 1, 237 codons. Almost all variation occurred outside of conserved functional domains where resistance mutations have been identified.
Chou
S
S
VA Medical Center, Portland, Oregon 97201, USA. chous@ohsu.edu
Lurain
N S
NS
Weinberg
A
A
Cai
G Y
GY
Sharma
P L
PL
Crumpacker
C S
CS
D000998:Antiviral Agents / Q000494:pharmacology*; D003062:Codon; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000235:genetics; D004352:Drug Resistance, Microbial; D017245:Foscarnet / Q000494:pharmacology; D015774:Ganciclovir / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D017386:Sequence Homology, Amino Acid
D000998:Antiviral Agents; D003062:Codon; D017245:Foscarnet; D004259:DNA-Directed DNA Polymerase; D015774:Ganciclovir