title: [Laser correlation spectroscopy of blood plasma for diagnosis of postradiation sequels].
abstract: The method of laser correlation spectroscopy (LCS) of biological fluids, permit to detect fractional composition of biological fluids (in this case--blood plasma). This method have been used to study the late effects of radiation in experimental investigations on animals (irradiation in doses that induce the high frequency of malignant tumors) and on the people living in the zone of Ural Radiation Trace. LCS spectra analysis show the proportion of separate ingredients in the blood plasma in the very bright range of molecular dimensions (d = 1-10000 nm) and for this analysis the special way of classification (semiotic classification) have been proposed. Multicomponent spectra, represented as a histogram (the abscess--diameters of light-scattering particles, ordinate--their per cent in the light-scattering of the whole sample) divide on the separate a priori marked zones with the appropriate means of diameters light-scattering subcomponent. In dependence on the increasing, or decreasing of enclosing in the light-scattering separated subcomponent the program of classification refer this histogram to separate cluster. Each cluster on the basis of preliminary investigations of different pathologic states related to the definite sign reflecting the type of homeostatic alterations (intoxication, allergic, dystrophic, catabolic and their combinations). The detection of the direction of homeostatic alteration and its degree is the main purpose of the semiotic classification of LC-spectra. In this paper it was shown that this way of diagnostic of the nature of homeostatic alterations in the blood plasma is more informative in the evaluation of the weightiness of pathologic state of organism in the comparison with the complexes of laboratory methods, usually used. The method can register immunoglobulin relations, changes in concentrations of lipoproteids and cholesterol and fractional composition of immunocompetent blood cells. The model experiments on animals irradiated in the doses that induce the high frequency of malignant tumors in 12 months thereafter (50 per cent of irradiated rats) have been conducted. With the help of LCS blood plasma it is possible to show the differences in direction of homeostatic changes in animals with and without oncological disease in a long period of time before pathomorphological registration of tumors. At last when 764 individuals living in the Zone of Ural radiation trace have been inspected by the LCS blood plasma it was shown: a. individuals without pathological diagnosis (in the range of 100-150 cz cumulating doses) the radiation action doesn't modify the homeostasis system in practice; b. in individuals with preoncological, oncological, autoimmune and metabolical diseases the modification of homeostasis system in these doses range is noticed. The direction of this modification depend on the nature of pathological process. The methodical simplicity of LCS investigations, rapidity and automatism of analysis together with informative sensitivity of this method testify to perspectives use of LCS blood plasma for the decision of the problem of biodosimetry of irradiation of experimental animals and for monitoring of late effects after low dose irradiation.
authors.affiliation: Ural Institute of Radiation Medicine, Health Ministry of Russia, Chelyabinsk.
authors.forename: M F
authors.initials: MF
authors.lastname: Kiselev
authors.forename: A V
authors.initials: AV
authors.lastname: Akleev
authors.forename: I A
authors.initials: IA
authors.lastname: Pashkov
authors.forename: N V
authors.initials: NV
authors.lastname: Klopov
authors.forename: V A
authors.initials: VA
authors.lastname: Noskin
authors.forename: L A
authors.initials: LA
authors.lastname: Noskin
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007834:Lasers; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000145:classification / Q000175:diagnosis; D010949:Plasma; D011230:Precancerous Conditions / Q000145:classification / Q000175:diagnosis; D011832:Radiation Injuries / Q000145:classification / Q000175:diagnosis*; D051381:Rats; D013057:Spectrum Analysis / Q000379:methods*