Fast C18 solid-phase desalting/delipidation of the human serum apolipoproteins for matrix-assisted laser desorption ionization and electrospray ionization mass spectrometric analysis.
A new method for the delipidation of human serum lipoproteins involving the use of a reversed-phase C18 solid-phase extraction (SPE) cartridge is introduced for use with matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry. This method is compared with two other methods of lipoprotein delipidation. The SPE method of delipidation produces a higher and more reproducible protein yield than the conventional liquid-liquid methanol-diethyl ether delipidation technique. Furthermore, the SPE method implements a fast, sequential, desalting and delipidation of the lipoproteins for subsequent mass spectrometric analysis providing high quality spectra.
Watkins
L K
LK
Texas A&M University, Department of Chemistry, College Station 77842-3012, USA.
Bondarenko
P V
PV
Barbacci
D C
DC
Song
S
S
Cockrill
S L
SL
Russell
D H
DH
Macfarlane
R D
RD
D001053:Apolipoproteins / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D011804:Quinolines / Q000032:analysis; D012016:Reference Values; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014461:Ultracentrifugation
D001053:Apolipoproteins; D007202:Indicators and Reagents; D008055:Lipids; D008075:Lipoproteins, HDL; D011804:Quinolines; D012997:Solvents; C047117:bicinchoninic acid