title: Dual specificity antibodies using a double-stranded oligonucleotide bridge.
abstract: The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The antibodies were then allowed to hybridize by base pairing of the complementary nucleotide sequences and the generation of bispecific antibody was analyzed on SDS-PAGE and confirmed using BIAcore analysis. The strategy of complementary oligonucleotide-linked bispecific molecules is not limited to antibodies but is applicable to linking any two molecules of different characteristics.
authors.affiliation: Department of Immunology, University College London, The Windeyer Institute for Medical Sciences, UK.
authors.forename: Z N
authors.initials: ZN
authors.lastname: Chaudri
authors.forename: M
authors.initials: M
authors.lastname: Bartlet-Jones
authors.forename: G
authors.initials: G
authors.lastname: Panayotou
authors.forename: T
authors.initials: T
authors.lastname: Klonisch
authors.forename: I M
authors.initials: IM
authors.lastname: Roitt
authors.forename: T
authors.initials: T
authors.lastname: Lund
authors.forename: P J
authors.initials: PJ
authors.lastname: Delves
mesh_terms: D000818:Animals; D018033:Antibodies, Bispecific / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015374:Biosensing Techniques; D004247:DNA / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D051379:Mice; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D018033:Antibodies, Bispecific; D007140:Immunoglobulin Fab Fragments; D009841:Oligonucleotides; D004247:DNA