Species-specific distribution of alpha-galactosyl epitopes on the gastric H/K ATPase beta-subunit: relevance to the binding of human anti-parietal cell autoantibodies.
The gastric H/K ATPase beta-subunit, an abundant glycoprotein of the secretory membranes of gastric parietal cells, is the major autoantigen recognized by human parietal cell autoantibodies in gastric autoimmunity. Our previous studies demonstrated that the human autoantibodies recognize the H/K ATPase beta-subunit from a number of species and that glycosylation of the beta-subunit with complex N-glycans is required for autoantibody binding. The N-glycans of the beta-subunit contain polylactosamine chains. The lactosamine chains of the rabbit beta-subunit are terminated with alpha-linked galactosyl residues (alpha-galactosyl epitope) (Tyagarajan et al., Biochemistry, 1996, 35, 3238-3246). Here we have investigated the expression of alpha-galactosyl epitopes on the H/K ATPase beta-subunit from a number of species. Using the alpha-galactosyl binding lectin, BS1-IB4, and naturally occurring anti-alpha-galactosyl antibodies, we have demonstrated that the rat H/K ATPase beta-subunit also contains terminal alpha-galactosyl residues, but not the beta-subunit from pig, dog, and mouse, indicating species-specific differences in the terminal saccharide sequences of the beta-subunit. We also investigated the potential contribution of the alpha-galactosyl epitopes to the binding by human sera. The reactivity of human pernicious anemia serum with gastric parietal cells could not be inhibited with saccharide inhibitors and, in addition, no binding was observed with normal human sera. We conclude that the H/K ATPase beta-subunit oligosaccharides from rabbit and rat are terminated with alpha-galactosyl epitopes, and although the presence of this epitope does not contribute to binding by human parietal cell autoantibodies at the concentrations routinely used, it is recommended that neither rat or rabbit stomachs be used for screening human sera.
Stewart
L A
LA
Department of Pathology and Immunology, Monash University Medical School, Melbourne, Victoria, Australia.
van Driel
I R
IR
Toh
B H
BH
Gleeson
P A
PA
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D000939:Epitopes / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D017506:H(+)-K(+)-Exchanging ATPase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D010295:Parietal Cells, Gastric / Q000276:immunology*; D013045:Species Specificity; D013270:Stomach / Q000201:enzymology* / Q000276:immunology
D001323:Autoantibodies; D000939:Epitopes; D017506:H(+)-K(+)-Exchanging ATPase; D005690:Galactose