Early detection of human cytomegalovirus infection after kidney transplantation by nucleic acid sequence-based amplification.
BACKGROUND
The early detection of human cytomegalovirus infection after organ transplantation is a prerequisite for effective antiviral therapy. We evaluated the diagnostic value of monitoring the viral immediate-early (IE) 1 mRNA expression in blood leukocytes by nucleic acid sequence-based amplification (NASBA).


METHODS
Nucleic acids were isolated from 489 blood samples collected from 42 kidney transplant recipients and subjected to amplification by IE NASBA. The IE NASBA results were compared to those from pp67 NASBA, pp65 antigenemia, cell culture (DEAFF and CPE), and serology.


RESULTS
IE NASBA proved to be the most sensitive assay which detected the onset of both primary and secondary cytomegalovirus infection significantly earlier than the other assays.


CONCLUSIONS
The early detection of cytomegalovirus infection with IE NASBA would enable the start of effective antiviral therapy at an early state of infection to prevent cytomegalovirus disease in patients at risk.
Blok
M J
MJ
Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.
Christiaans
M H
MH
Goossens
V J
VJ
van Hooff
J P
JP
Sillekens
P
P
Middeldorp
J M
JM
Bruggeman
C A
CA
D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis* / Q000517:prevention & control; D004279:DNA, Viral / Q000097:blood; D005784:Gene Amplification; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis; D016030:Kidney Transplantation / Q000009:adverse effects*; D009696:Nucleic Acids / Q000097:blood*; D011446:Prospective Studies; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D014764:Viral Proteins / Q000096:biosynthesis
D004279:DNA, Viral; D017874:Immediate-Early Proteins; D009696:Nucleic Acids; D012333:RNA, Messenger; D014764:Viral Proteins