Protein kinase C regulates NO-cGMP pathway in muscarinic receptor activation by HIV+-IgA.
In this work we demostrate that IgA purified from HIV infected patients recognizes a band with a molecular weight corresponding to radiolabelled ileal muscarinic acethylcholine receptors (mAChR) by immunoblotting. HIV+-IgA triggers the signals that are the consequence of mAChR stimulation in the intestine, regulating protein kinase C (PKC) and nitric oxide synthase (NOS) activity as well as 3',5'-cyclic guanosine monophosphate (cGMP) formation. On the one hand PKC activation by HIV+-IgA induces NOS inhibition and as a consequence, low amounts of NO that could improve local immunosuppression in the intestine; on the other hand HIV+-IgA stimulates cGMP production which could potentiate ileal motility and loss of water/electrolytes involved in intestinal damage in AIDS.
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Instituto de Oncologia Angel H. Roffo, Buenos Aires, Argentina.
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Sterin-Borda
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Borda
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de Bracco
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D000818:Animals; D000918:Antibody Specificity; D006152:Cyclic GMP / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007082:Ileum / Q000378:metabolism; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000097:blood / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D011493:Protein Kinase C / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000378:metabolism*; D015398:Signal Transduction
D004791:Enzyme Inhibitors; D007070:Immunoglobulin A; D011976:Receptors, Muscarinic; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D011493:Protein Kinase C; D006152:Cyclic GMP