Microtitre plate and assay buffer alter detection of antiphosphatidylethanolamine antibodies in lupus anticoagulant positive plasma.
The ELISA for detection of anti-phosphatidylethanolamine antibodies in patients with lupus anticoagulant was studied. The influence of microtitre plate types, antigen concentration, antibody dilution, and buffer system was evaluated. Human albumin in the blocking buffer gave greater numbers of positive plasma samples than bovine serum. Comparison of simultaneous plasma and serum samples showed only minor variation in binding characteristics.
Drouvalakis
K A
KA
The University of Melbourne, Department of Medicine, Austin & Repatriation Medical Centre, Heidelberg, Victoria, Australia. drouvaka@leland.stanford.edu
Buchanan
R R
RR
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002021:Buffers; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000592:standards*; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000097:blood / Q000276:immunology*; D010714:Phosphatidylethanolamines / Q000276:immunology*; D012680:Sensitivity and Specificity
D000906:Antibodies; D002021:Buffers; D016682:Lupus Coagulation Inhibitor; D010714:Phosphatidylethanolamines