Expanding the catalytic repertoire of nucleic acid catalysts: simultaneous incorporation of two modified deoxyribonucleoside triphosphates bearing ammonium and imidazolyl functionalities.
Two nucleoside triphosphates, a pyrimidine modified with an ammonium functionality and a purine modified with an imidazolyl functionality are compatible with all conditions for a combinatorial selection of nucleic-acid catalysts. We believe that this work is the first to demonstrate the potential for using not one but two modified nucleotides in tandem. The potential for an enriched catalytic repertoire is envisioned.
Perrin
D M
DM
Laboratoire de Biophysique, Museum National d'Histoire Naturelle, Paris, France.
Garestier
T
T
Hélène
C
C
D017123:Bacteriophage T7 / Q000201:enzymology; D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D017931:DNA Primers / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase; D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013698:Templates, Genetic
D017931:DNA Primers; D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase