Inhibitory effects of indomethacin on interleukin-1 and nitric oxide production in rat microglia in vitro.
Indomethacin, as a nonsteroidal antiinflammatory drug, is reported to be effective in some degree in the prevention and treatment of Alzheimer's disease (AD). Effects of indomethacin on proinflammatory cytokines interleukin-1 (IL-1), tumor necrosis factor alpha and nitric oxide (NO) on rat microglia in vitro were investigated. The biological activities of IL-1 were assayed by thymocyte proliferation assay and the activities of TNG-alpha, by L929 cytotoxicity assay. NO concentration was represented by nitrite and determined by Griess reaction. Indomethacin inhibited IL-1 and NO production by rat microglia stimulated at the concentration of 0.1-10 micromol/l. However, it did not show any inhibitory effect on TNF-alpha production by resting and LPS-stimulated rat microglia. The results suggest that the mechanism by which indomethacin might be beneficial in treatment of AD might be due to the inhibition IL-1 and NO production from microglia.
Du
Z Y
ZY
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Li
X Y
XY
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002478:Cells, Cultured; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007375:Interleukin-1; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007213:Indomethacin