Differentiation and assessment of cell death.
Three documented cell death pathways, apoptosis, necrosis, and oncosis will be discussed. The end result of each pathway is cell death; however, the path by which death is achieved and the morphological and physiological traits of each may be strikingly distinct. Now that well characterized models have been established for particularly apoptosis, the induction pathway(s) has received much attention and the pathway pathology is beginning to be understood. Three model systems were investigated: APO-1/Fas, hypoxia, and oncosis. Cell death was induced, and during a time course sampling, a variety of methodologies, including DNA fragmentation by flow cytometry and gel electrophoresis, DNA staining, flow cytometric light scatter, transmission electron microscopy, anti-tubulin, Trypan blue, annexin V, and anti-APO2.7 were employed to monitor the cell death progress. The apoptotic pathway in the CD95-induced Jurkat cell model was further investigated using caspase inhibitor peptides and analyzed for APO2.7 antigen expression and DNA fragmentation by flow cytometry. Time course sampling characterized the cell death pathway and helped to differentiate the capabilities of the methods. The time to response and duration of the response were dependent upon cell type and method of induction. The CD95-induced Jurkat cell model showed a classical apoptotic response; however the MDA-MB-175-VII hypoxia model and the anti-5A9 induced oncosis model were not as clear. Each methodology shows advantages and disadvantages that allow the investigator to select several methods to identify, monitor, and enumerate cells with respect to cell death progression using time course studies.
Koester
S K
SK
Advanced Technology, Beckman Coulter, Inc., Miami, Florida 33196-2500, USA.
Bolton
W E
WE
D016923:Cell Death / Q000276:immunology; D002454:Cell Differentiation; D005434:Flow Cytometry; D006801:Humans; D008027:Light; D008563:Membrane Lipids / Q000378:metabolism; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism; D012542:Scattering, Radiation; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D008563:Membrane Lipids; D010743:Phospholipids