A chemogenetic approach to RNA function/structure analysis.
Almost two dozen nucleotide analogs have been synthesized with alpha-phosphorothioate-tagged triphosphates and utilized in an interference modification approach termed Nucleotide Analog Interference Mapping. This method has made it possible to determine the chemical basis of RNA function and structure, including the identification of new rules for RNA helix packing, the functional analysis of a binding site for monovalent metal ions within RNA and the characterization of the catalytic mechanism of RNA enzymes.
Strobel
S A
SA
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA. strobel@csb.yale.edu
D000818:Animals; D020029:Base Pairing; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics; D013329:Structure-Activity Relationship; D013768:Tetrahymena / Q000737:chemistry / Q000235:genetics; D013873:Thionucleotides / Q000737:chemistry
D009705:Nucleosides; D016337:RNA, Catalytic; D013873:Thionucleotides; D012313:RNA