Optimization of the cost and sensitivity of receptor- and enzyme-based assays.
In detecting receptor antagonists or enzyme inhibitors, there are three parameters that often affect the outcome in a predictable quantitative manner: concentrations of the receptors (enzyme), labeled ligand (substrate), and antagonist (inhibitor). The usual goal of assay optimization is to maximize the ability of the assay to detect low concentrations of the analyte. Another question of practical importance, especially in screening of large numbers of samples, would be minimization of the reagent cost. Although the mathematical theory of optimization of the receptor binding assay was developed a long time ago, the resulting formulas (in the general case of unequal affinities of ligand and competitor) were not well suited for practical use. The current availability of computational programs, such as Mathematica, makes possible an efficient solution, both for receptor- and enzyme-based assays. We use a graphical approach to assay optimization and apply it to the following problems: (1) optimization of assay sensitivity, (2) optimization of the reagent cost, and (3) analysis of the entire range of the parameter values since the mathematically optimal values may sometimes be impractical. The computation is extremely simple and the problem can sometimes be solved in several minutes.
Model
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Division of Biological Materials, Northwestern University, Chicago, Illinois 60611, USA. mmodel@nwu.edu
Healy
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D001667:Binding, Competitive; D001699:Biometry; D002384:Catalysis; D003365:Costs and Cost Analysis; D004791:Enzyme Inhibitors / Q000032:analysis; D004798:Enzymes / Q000032:analysis*; D005069:Evaluation Studies as Topic; D008024:Ligands; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000037:antagonists & inhibitors; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis* / Q000037:antagonists & inhibitors; D012680:Sensitivity and Specificity; D012984:Software
D004791:Enzyme Inhibitors; D004798:Enzymes; D008024:Ligands; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear