Cell culture test of TCP/CPLA composite.
Thermoplastic polymers filled with a ceramic powder (here described as a "composite") of beta-tricalcium phosphate (TCP) and copoly-L-lactide (CPLA) was prepared by a heat-kneading method. The TCP/CPLA composite obtained had a three-point bending strength of 51.26 +/- 634 MPa, a Young's modulus of 5.18 +/- 1.11 GPa, and a fracture strength of 52.64 +/- 2.975 MPa, which is sufficient for usage as an artificial bone implant material. According to cell culture tests using MC3T3-E1 cells, the TCP/CPLA composite showed no cytotoxicity and the cells were in close contact with the surfaces of the composite without any observable morphological change. These results suggest that a composite consisting of osteoconductive TCP and biodegradable CPLA is suitable as a bioactive artificial bone material.
Kikuchi
M
M
National Institute for Research in Inorganic Materials, Ibaraki, Japan. kikuchi@nirim.go.jp
Tanaka
J
J
Koyama
Y
Y
Takakuda
K
K
D020341:Absorbable Implants / Q000009:adverse effects; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000633:toxicity; D018929:Cell Culture Techniques; D002516:Ceramics / Q000633:toxicity; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D011091:Polyesters / Q000633:toxicity
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011091:Polyesters; C485817:beta-tricalcium phosphate; C033616:poly(lactide); C018392:tricalcium phosphate