Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer.
NFkappaB plays an important role in mediating the gene expression of numerous cellular processes such as growth, development, the inflammatory response and virus proliferation. The p50/p65 heterodimer is the most abundant form of the NFkappaB dimers and plays a more elaborate role in gene regulation. Biochemical research on p50/p65 NFkappaB has not benefited however from the availability of easily purified recombinant protein. We report two methods for the large scale expression and purification of recombinant NFkappaB p50/p65 heterodimer. The first utilizes a bacterial double expression vector which contains two ribosomal binding sites to facilitate the coexpression of the polypeptides in the p50/p65 NFkappaB heterodimer. The second method uses a mixed protein refolding strategy. Both methods yield crystallizable protein. Electrophoretic mobility shift assays confirm that the DNA binding affinity is independent of the method used to purify the protein. These methods will facilitate the numerous studies on various NFkappaB/Rel family members.
Chen
F E
FE
Department of Biology, University of California at San Diego, La Jolla, CA 92037, USA.
Kempiak
S
S
Huang
D B
DB
Phelps
C
C
Ghosh
G
G
D001665:Binding Sites / Q000235:genetics; D003460:Crystallization; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D019281:Dimerization; D004926:Escherichia coli; D005786:Gene Expression Regulation; D016328:NF-kappa B / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
D004268:DNA-Binding Proteins; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins