Detection and expression of corticosteroid binding protein gene in human pathogenic fungi.
Participation of steroid hormones in the growth of several fungal species has been widely reported. The aim of the present study was to detect the presence and expression of the corticosteroid binding protein (CBP) gene in different pathogenic fungal isolates from human clinical specimens. Genomic DNA and total RNA were obtained from six different pathogenic fungal species and submitted to Southern blot and Reverse Transcription-Polymerase Chain Reaction respectively. The results indicated that all the fungi studied presented and expressed CBP gene. The sequence of a PCR product of CBP gene fragment corresponding to the carboxyl terminal region in Trichophyton mentagrophytes, which presented the highest CBP expression, showed an identity of 98% as compared to the previously reported gene sequence from Candida albicans. The overall results indicate that CBP is a highly conserved gene in fungi and suggest that steroid hormones should play an important physiological role in these eukaryotic organisms.
Hernández-Hernández
F
F
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México. franciscahh@liceaga.facmed.unam.mx
López-Martínez
R
R
Camacho-Arroyo
I
I
Mendoza-Rodríguez
C A
CA
Cerbón
M A
MA
D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D015139:Blotting, Southern; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003345:Corticosterone; D015870:Gene Expression; D005800:Genes, Fungal; D006801:Humans; D003904:Mitosporic Fungi / Q000235:genetics* / Q000378:metabolism; D009181:Mycoses / Q000382:microbiology*; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000305:Adrenal Cortex Hormones; D002352:Carrier Proteins; D012331:RNA, Fungal; D003345:Corticosterone