title: Structure of VAT, a CDC48/p97 ATPase homologue from the archaeon Thermoplasma acidophilum as studied by electron tomography.
abstract: Valosine-containing protein-like ATPase from Thermoplasma acidophilum is a member of the superfamily of ATPases associated with a diversity of cellular activities and is closely related to CDC48 from yeast and p97 from higher eukaryotes and more distantly to N-ethylmaleimide-sensitive fusion protein. We have used electron tomography to obtain low-resolution (2-2.5 nm) three-dimensional maps of both the whole 500 kDa complex and the N-terminally truncated valosine-containing protein-like ATPase from T. acidophilum complex lacking the putative substrate binding domain.
authors.affiliation: Max-Planck-Institut für Biochemie, Abteilung für molekulare Strukturbiologie, Martinsried, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Rockel
authors.forename: J
authors.initials: J
authors.lastname: Walz
authors.forename: R
authors.initials: R
authors.lastname: Hegerl
authors.forename: J
authors.initials: J
authors.lastname: Peters
authors.forename: D
authors.initials: D
authors.lastname: Typke
authors.forename: W
authors.initials: W
authors.lastname: Baumeister
mesh_terms: D000251:Adenosine Triphosphatases / Q000648:ultrastructure*; D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D008854:Microscopy, Electron; D013822:Thermoplasma / Q000201:enzymology*; D000074405:Valosin Containing Protein
chemical_list: D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein; C105134:vat protein, Thermoplasma acidophilum