Monoclonal anti-idiotype antibody mimicking the pesticide binding site of cutinase: potential for broad specificity organophosphate recognition.
An anti-idiotype monoclonal antibody (Mab) able to bind the organophosphate pesticides, chlorfenvinphos (CFV), ethyl paraoxon, tetrachlorfenvinphos and demeton-s-methyl, has been produced using as immunogen a Mab which binds to the active site of cutinase. The principle of using an anti-idiotype antibody as the mimic of a site on a protein able to bind a group of ligands has, therefore, been demonstrated, and may have implications for future research on broad specificity immunoanalysis of groups of compounds.
Ward
C M
CM
Department of Biochemistry, Institute of Food Research, Norwich Research Park, Colney, UK.
Wilkinson
A P
AP
Lee
H A
HA
Morgan
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001665:Binding Sites; D002265:Carboxylic Ester Hydrolases / Q000276:immunology*; D005670:Fusarium / Q000201:enzymology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007306:Insecticides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009943:Organophosphorus Compounds
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007306:Insecticides; D009943:Organophosphorus Compounds; D002265:Carboxylic Ester Hydrolases; C019952:cutinase