title: Macromolecular docking of a three-body system: the recognition of human growth hormone by its receptor.
abstract: Human growth hormone (hGH) binds to its receptor (hGHr) in a three-body interaction: one molecule of the hormone and two identical monomers of the receptor form a trimer. Curiously, the hormone-receptor interactions in the trimer are not equivalent and the formation of the complex occurs in a specific kinetic order (Cunningham BC, Ultsch M, De Vos AM, Mulkerrin MG, Clauser KR, Wells JA, 1991, Science 254:821-825). In this paper, we model the recognition of hGH to the hGHr using shape complementarity of the three-dimensional structures and macromolecular docking to explore possible binding modes between the receptor and hormone. The method, reported previously (Hendrix DK, Kuntz ID, 1998, Pacific symposium on biocomputing 1998, pp 1234-1244), is based upon matching complementary-shaped strategic sites on the molecular surface. We modify the procedure to examine three-body systems. We find that the order of binding seen experimentally is also essential to our model. We explore the use of mutational data available for hGH to guide our model. In addition to docking hGH to the hGHr, we further test our methodology by successfully reproducing 16 macromolecular complexes from X-ray crystal structures, including enzyme-inhibitor, antibody-antigen, protein dimer, and protein-DNA complexes.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco, 94143-0446, USA.
authors.forename: D K
authors.initials: DK
authors.lastname: Hendrix
authors.forename: T E
authors.initials: TE
authors.lastname: Klein
authors.forename: I D
authors.initials: ID
authors.lastname: Kuntz
authors.forename: T E
authors.initials: TE
authors.lastname: Klien
mesh_terms: D000465:Algorithms; D001665:Binding Sites; D003198:Computer Simulation; D019382:Human Growth Hormone / Q000737:chemistry*; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D011485:Protein Binding
chemical_list: D019382:Human Growth Hormone