Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales.
The overexpression of E-FABP and S100A7 in lesional psoriatic skin suggests a possible link with this hyperproliferative skin disease. In order to investigate a role for the proteins in this disease, the purifications for both proteins were re-analyzed. Moreover, a specific antiserum directed against purified human S100A7 was generated. By SDS-PAGE immunoblotting we show that E-FABP and S100A7 are expressed in cultured human differentiating keratinocytes and confirm their overexpression in psoriatic scales. Gel filtration and non-denaturing PAGE revealed that S100A7 co-purified with E-FABP, indicating an association between the two proteins. Ion-exchange chromatography resulted in the dissociation of the complex. Finally, immunoprecipitations using antiserum against E-FABP revealed that S100A7 co-immunoprecipitated with E-FABP from protein extracts of psoriatic scales. These data indicate that E-FABP and S100A7 might form a complex in the cytosol of human keratinocytes.
Hagens
G
G
Department of Dermatology and DHURDV, University Hospital, Geneva, Switzerland.
Roulin
K
K
Hotz
R
R
Saurat
J H
JH
Hellman
U
U
Siegenthaler
G
G
D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D018996:Myelin P2 Protein / Q000378:metabolism*; D009363:Neoplasm Proteins; D011233:Precipitin Tests; D011485:Protein Binding; D011565:Psoriasis / Q000378:metabolism*; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; C493412:FABP5 protein, human; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; D025521:Tumor Suppressor Proteins