Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.
The Arabidopsis thaliana NPR1 has been shown to be a key regulator of gene expression during the onset of a plant disease-resistance response known as systemic acquired resistance. The npr1 mutant plants fail to respond to systemic acquired resistance-inducing signals such as salicylic acid (SA), or express SA-induced pathogenesis-related (PR) genes. Using NPR1 as bait in a yeast two-hybrid screen, we identified a subclass of transcription factors in the basic leucine zipper protein family (AHBP-1b and TGA6) and showed that they interact specifically in yeast and in vitro with NPR1. Point mutations that abolish the NPR1 function in A. thaliana also impair the interactions between NPR1 and the transcription factors in the yeast two-hybrid assay. Furthermore, a gel mobility shift assay showed that the purified transcription factor protein, AHBP-1b, binds specifically to an SA-responsive promoter element of the A. thaliana PR-1 gene. These data suggest that NPR1 may regulate PR-1 gene expression by interacting with a subclass of basic leucine zipper protein transcription factors.
Zhang
Y
Y
Developmental, Cell, and Molecular Biology Group, Department of Botany, Box 91000, Duke University, Durham, NC 27708-1000, USA.
Fan
W
W
Kinkema
M
M
Li
X
X
Dong
X
X
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D016367:Baculoviridae; D005656:Fungal Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics; D016350:Leucine Zippers; D018551:Solanum lycopersicum / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D020156:Salicylic Acid / Q000494:pharmacology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D005656:Fungal Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C053376:pathogenesis-related proteins, plant; C066958:NPR1 protein, S cerevisiae; D011494:Protein Kinases; D006026:Glycoside Hydrolases; D020156:Salicylic Acid