title: [Isolating candidate inserted fragment from positive fused phage clones using quantitative PCR].
abstract: OBJECTIVE
  To isolate quickly and exactly the specific inserted fragment from fused phage clones which were obtained from cDNA library by hybridization.
  
  
  METHODS
  According to the amplification principle of quantitative polymerase chain reaction (PCR) and based on the difference of original template quantity,target cDNA fragment was isolated and identified by two PCRs.
  
  
  RESULTS
  A positive clone with specific cDNA fragment of HumGT-H1 gene was obtained from a two-phage fused clone by using this method,and the inserted fragment was verified to be the 5' cDNA sequence of HumGT-H1,1.9kb in length. So another hybridization screening is not necessary.
  
  
  CONCLUSIONS
  The method presented is effective and rapid in gene cloning and can greatly save time and materials.
authors.affiliation: Institute of Genetics,Fudan University, Shanghai,200433 P.R. China. Longyu@fudan.edu.cn
authors.forename: Y
authors.initials: Y
authors.lastname: Fan
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: F
authors.initials: F
authors.lastname: Dai
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Dong
authors.forename: S
authors.initials: S
authors.lastname: Zhao
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D018076:DNA, Complementary / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary