[Genetic polymorphism at FES locus in Chinese and German populations].
OBJECTIVE
The aim of this study on the genetic polymorphism at FES locus was to know whether there is genetic relationship between Chinese and German populations.


METHODS
EDTA-blood specimens were collected from 311 healthy unrelated Han individuals in Jilin, Chengdu and Guangzhou in China and from 123 healthy unrelated individuals in Germany. The DNA samples were extracted using Chelex method and amplified by PCR technique. The PAGE horizontal electrophoresis was used for typing the PCR product.DNA sequences were analyzed by ALF.


RESULTS
There were nine alleles at FES locus in the Chinese population. The allele frequencies were FES*6,0-0. 0051; FES *8,0-0.0042; FES*9,0.0083-0.0202; FES*10,0.0202-0.0604; FES*11,0.4066-0.5101; FES*12,0.2424-0.3099;FES*13, 0.1860-0.2198; FES*14,0.0041-0.0165; and FES*15,0-0.0050, respectively. In the German population, a variation in 5 flanking region and nine alleles at FES locus were noted. The allele frequencies were FES*8,0.0120; FES*10a,0.02317; FES*10,0.0407; FES*11a,0.0122; FES*11,0.4146; FES*12a, 0.0041; FES*12, 0.2357; FES*13, 0.0447; and FES*14, 0.0041, respectively. The results of test for Hardy-Weinberg equilibrium showed that the genotype distributions observed in the populations were correspondent with the expected.


CONCLUSIONS
There is a remarkable difference in the distribution of allele frequencies at FES locus between Chinese and German populations.
Wu
J
J
Institute for Forensic Medicine,West China University of Medical Sciences, Chengdu,610041 P.R.China. rechtsme@wcums.edu.cn
Hou
Y
Y
Li
Y
Y
Prinz
M
M
Staak
S
S
D000483:Alleles; D002681:China / Q000208:ethnology; D002874:Chromosome Mapping; D005787:Gene Frequency; D005858:Germany; D006801:Humans; D011110:Polymorphism, Genetic; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051578:Proto-Oncogene Proteins c-fes; D011519:Proto-Oncogenes
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496645:FES protein, human; D051578:Proto-Oncogene Proteins c-fes