Survival of cryopreserved fetal substantia nigra implanted into the rat brain in the two forms and estimation of the host brain reaction against graft.
A fetal, cryopreserved ventral mesencephalic rat tissue was grafted into striatum of 143 healthy adult rats, applying two different forms, solid tissue block or cell-suspension. The fetal tissue was preserved in liquid nitrogen for 30-80 days. For transplantation stereotaxic placement technique was employed. The controls were subjected to sham transplantation. The survival of transplanted dopaminergic cells in rat striatum was evaluated by means of histological and immunocytochemical methods (TH-tyrosine hydroxylase) 1, 3, 7, 14 and 21 days after transplantation. The host cellular reaction against the graft and sham-lesion was examined. Glial fibrillary acidic protein (GFAP) was used for the visualization of astroglial reaction and ferritin for microglia. The occurrence of T-lymphocytes was also assessed using W3/13 antibodies. Electron microscopy was utilized as well in the evaluation. It was found that fetal cells of cryopreserved rat mesencephalon transplanted into adult rat striatum in two different models survive and mature similarly. The host cellular reaction against the graft was nonspecific and similar to that found in the control groups. Over a posttransplantation period of 21 days no graft rejection was observed in any of the experimental groups.
Kosno-Kruszewska
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Department of Neuropathology, Institute of Psychiatry and Neurology, Warszawa.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000601:surgery*; D015925:Cryopreservation; D016332:Fetal Tissue Transplantation; D006084:Graft Rejection / Q000175:diagnosis*; D006789:Host vs Graft Reaction / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000166:cytology / Q000196:embryology* / Q000637:transplantation*; D014022:Tissue Survival