Role of interleukin-2 receptor expression on macrophages from Salmonella-infected mice.
In this study, it was found that macrophages from Salmonella-infected mice expressed the interleukin-2 receptor (IL-2R). The IL-2R induced by the infection consisted mainly of both beta and gamma chains, and the expression of IL-2Ralpha was very low. Furthermore, similar expression of IL-2Rbeta and gamma chains was observed in interferon-gamma-treated macrophages. The effect of recombinant interleukin-2 on the intracellular killing activity of macrophages which expressed IL-2Rbeta and gamma was then studied. Our results demonstrated that interleukin-2 acted synergistically with interferon-gamma through the IL-2R in the induction of macrophage intracellular killing activity and established a new role of IL-2R on bactericidal activity of macrophages.
Matsui
K
K
Department of Immunobiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. kmatsui@my-pharm.ac.jp
D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000502:physiology*; D012481:Salmonella Infections, Animal / Q000276:immunology; D012486:Salmonella typhimurium
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma