Membrane topology of MntB, the transmembrane protein component of an ABC transporter system for manganese in the cyanobacterium Synechocystis sp. strain PCC 6803.
The structure of the membrane protein MntB, a component of a manganese transporter system in Synechocystis sp. strain PCC 6803, was examined with a series of fusions to the reporter proteins alkaline phosphatase and beta-galactosidase. The results support a topological model for MntB consisting of nine transmembrane segments, with the amino terminus of the protein being in the periplasm and the carboxyl terminus being in the cytoplasm.
Bartsevich
V V
VV
Department of Biology, Washington University, St. Louis, Missouri 63130, USA.
Pakrasi
H B
HB
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins; D015153:Blotting, Western; D000458:Cyanobacteria / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter; D008345:Manganese / Q000378:metabolism; D008958:Models, Molecular; D019897:Periplasm / Q000378:metabolism; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; C119581:MntB protein, Synechocystis; D011993:Recombinant Fusion Proteins; D008345:Manganese; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase