Rapid mass spectrometric analysis for ascorbate and related organic acids in small volumes of plasma for use in pediatric subjects.
A gas chromatographic-mass spectrometric isotope dilution method was developed for analysis of ascorbate on 10 microl samples of plasma. This assay was reproducible (standard deviation of less than 4%) and gave values for plasma ascorbate content within 8% of our previously published gas chromatographic-mass spectrometric method. Non-specific sample preparation allowed other analytes to be determined on the same sample by adjusting data acquisition parameters and adding the appropriate internal standard. Analysis on 28 subjects fell within the expected range for plasma ascorbate 68+/-29 microm (11.9+/-5.0 microg/ml) and established a normal range for plasma threonate of 28.1+/-2.4 microm (3.8+/-0.4 microg/ml).
Deutsch
J C
JC
Department of Medicine, Denver Veterans Affairs Hospital and University of Colorado Health Sciences Center, 80220, USA. deutschj@essex.uchsc.edu
Butler
J A
JA
Marsh
A M
AM
Ross
C A
CA
Norris
J M
JM
D001205:Ascorbic Acid / Q000097:blood*; D002138:Calibration; D002675:Child, Preschool; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007223:Infant; D012015:Reference Standards; D012016:Reference Values; D015203:Reproducibility of Results; D013912:Threonine / Q000097:blood*
D013912:Threonine; D001205:Ascorbic Acid