Adenovirus-mediated introduction of DNA into pig sperm and offspring.
The ability of adenoviral vectors to transfer DNA into boar spermatozoa and to offspring was tested. Exposure of spermatozoa to adenovirus bearing the E. coli lacZ gene resulted in the transfer of the gene to the head of the spermatozoa. Treatment did not affect either viability or acrosomal integrity of boar sperm. Of the 2-to 8-cell embryos obtained after in vitro fertilization with adenovirus-exposed sperm, 21.7% expressed the LacZ product. Four out of 56 piglets (about 7%) obtained after artificial insemination with adenovirus-exposed spermatozoa were positive in PCR analyses, even though none of the piglets showed the LacZ gene after southern blot analysis. RT-PCR analysis performed in tissues from two positive stillborn piglets showed the presence of the LacZ mRNA in all of the tissues tested. The offspring obtained after mating two positive animals did not show LacZ gene presence. Our results indicate that adenovirus could be a feasible mechanism for the delivery of DNA into spermatozoa, even though the transfer of the transgene may be limited to the first generation.
Farre
L
L
Department of Biochemistry and Molecular Biology, School of Chemistry, University of Barcelona, Spain.
Rigau
T
T
Mogas
T
T
García-Rocha
M
M
Canal
M
M
Gomez-Foix
A M
AM
Rodríguez-Gil
J E
JE
D000177:Acrosome; D000256:Adenoviridae / Q000502:physiology; D000818:Animals; D004247:DNA; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D007315:Insemination, Artificial; D007763:Lac Operon; D008297:Male; D013094:Spermatozoa; D013552:Swine; D014779:Virus Replication
D004247:DNA