Different types of maize histone deacetylases are distinguished by a highly complex substrate and site specificity.
Enzymes involved in histone acetylation have been identified as important transcriptional regulators. Maize embryos contain three histone deacetylase families: RPD3-type deacetylases (HD1-B), nucleolar phosphoproteins of the HD2 family, and a third form unrelated to RPD3 and HD2 (HD1-A). Here we first report on the specificity of deacetylases for core histones, acetylated histone H4 subspecies, and acetylated H4-lysine residues. HD1-A, HD1-B, and HD2 deacetylate all four core histones, although with different specificity. However, experiments with histones from different sources (hyperacetylated MELC and chicken histones) using antibodies specific for individually acetylated H4-lysine sites indicate that the enzymes recognize highly distinct acetylation patterns. Only RPD3-type deacetylase HD1-B is able to deacetylate the specific H4 di-acetylation pattern (position 12 and 5) introduced by the purified cytoplasmic histone acetyltransferase B after incubation with pure nonacetylated H4 subspecies. HD1-A and HD2 exist as phosphorylated forms. Dephosphorylation has dramatic, but opposite effects; whereas HD2 loses enzymatic activity upon dephosphorylation, HD1-A is activated with a change of specificity against acetylated H4 subspecies. The data suggest that different types of deacetylases interact with different and highly specific acetylation patterns on nucleosomes.
Kölle
D
D
Department of Microbiology, University of Innsbruck, Medical School, Austria.
Brosch
G
G
Lechner
T
T
Pipal
A
A
Helliger
W
W
Taplick
J
J
Loidl
P
P
D000107:Acetylation; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D006655:Histone Deacetylases / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D006657:Histones / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D007527:Isoenzymes / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D012156:Reticulocytes / Q000201:enzymology; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured; D003313:Zea mays / Q000201:enzymology*
D006657:Histones; D007527:Isoenzymes; D006655:Histone Deacetylases