Cloning of the glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa.
The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti.
Franklund
C V
CV
Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.
Goldberg
J B
JB
D000595:Amino Acid Sequence; D002384:Catalysis / Q000187:drug effects; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005816:Genetic Complementation Test; D005975:Glutamate-tRNA Ligase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010618:Phenanthrolines / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D016962:Sinorhizobium meliloti / Q000201:enzymology / Q000235:genetics; D013696:Temperature; D015032:Zinc / Q000378:metabolism
D010618:Phenanthrolines; D011994:Recombinant Proteins; D005975:Glutamate-tRNA Ligase; D015032:Zinc; C025205:1,10-phenanthroline