title: Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei.
abstract: The start site of the A-type ribosomal RNA transcription units of the rodent malaria parasite, Plasmodium berghei, has been identified. The two A-type units cannot be distinguished within the transcription unit, yet exist as single copies on different chromosomes. Gene transcription initiates 820 bp upstream of the A-type small subunit (SSU) ribosomal gene and two major processing sites were mapped 610 and 611 nucleotides upstream of the SSU in the external transcribed spacer region. Surprisingly the nucleotide sequence of the DNA region containing the putative ribosomal promoter lacked repetitive DNA sequences typical of ribosomal promoters. This region was further analysed by computer using programs designed to reveal sequence-dependent structural features. Comparison of DNA curvature, duplex stability and pattern of twist angle variation revealed a striking degree of conservation between the ribosomal promoters from Plasmodium and other eukaryotes.
authors.affiliation: Department of Parasitology, University of Leiden, The Netherlands.
authors.forename: R M
authors.initials: RM
authors.lastname: van Spaendonk
authors.forename: G A
authors.initials: GA
authors.lastname: McConkey
authors.forename: J
authors.initials: J
authors.lastname: Ramesar
authors.forename: A
authors.initials: A
authors.lastname: Gabrielian
authors.forename: T F
authors.initials: TF
authors.lastname: McCutchan
authors.forename: C J
authors.initials: CJ
authors.lastname: Janse
authors.forename: A P
authors.initials: AP
authors.lastname: Waters
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D016053:RNA, Protozoan / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D016053:RNA, Protozoan