Assessment of mucosal immune response in genitourinary tract using urine.
A novel method to assess mucosal immune response in the genitourinary mucosa after immunization with a mucosal vaccine has been developed. In this method, secretory IgA antibody is measured by a highly sensitive enzyme immunoassay (immune-complex transfer enzyme immunoassay) using urine as a specimen. The urinary IgA antibody response could be detected by the immune-complex transfer enzyme immunoassay. In contrast, a conventional enzyme immunoassay (enzyme-linked immunosorbent assay (ELISA)) could not detect this response because of its low sensitivity. Because urine samples can be collected easily and nontraumatically, not only from experimental animals but also from humans, both males and females, the present method may be applicable for assessing the protective efficacy of candidates for mucosal vaccines against sexually transmitted microorganisms, such as human immunodeficiency virus. Furthermore, the usefulness of this method for novel mucosal vaccine formulae was shown for a model in which vaccine antigen and Bordetella pertussis adjuvant were adsorbed onto CaCO, and enclosed in enteric coated capsules.
Kohno
T
T
Department of Environmental Health Sciences, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan.
Tanaka
H
H
Watabe
K
K
Yamashita
S
S
Sezaki
H
H
Nadai
T
T
Sugie
Y
Y
Ogouchi
T
T
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018928:Immunity, Mucosal; D007124:Immunoenzyme Techniques; D007071:Immunoglobulin A, Secretory / Q000652:urine*; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D014566:Urogenital System / Q000276:immunology*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D007071:Immunoglobulin A, Secretory; D014612:Vaccines; D010047:Ovalbumin