title: Isolation of highly enriched human monocytes from ten ml samples of heparinised whole blood.
abstract: A detailed study of the immune dysregulation involved in systemic inflammation requires the analysis of the main inflammatory cells in the circulation - the monocytes. Blood samples available from patients are necessarily restricted so that a rapid and efficient method is required to isolate these cells. Here we present a protocol to isolate blood monocytes in high yield from small samples of heparinised blood. The method yields monocyte preparations with a purity greater than 96%.
authors.affiliation: Institut für Immunologie und Transfusionsmedizin, Universität Greifswald, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Wiegand
authors.forename: K
authors.initials: K
authors.lastname: Selleng
authors.forename: M
authors.initials: M
authors.lastname: Gründling
authors.forename: R S
authors.initials: RS
authors.lastname: Jack
mesh_terms: D002469:Cell Separation; D005434:Flow Cytometry; D006493:Heparin; D006801:Humans; D018189:Immunomagnetic Separation; D009000:Monocytes / Q000166:cytology*
chemical_list: D006493:Heparin