title: Surface pH at the basolateral membrane of the caecal mucosa of guinea pig.
abstract: Since the major mechanisms responsible for regulation of intracellular pH of enterocytes are located in the basolateral membrane, respective effects may be expected on pH in the compartment near the basolateral membrane. A method was established to estimate the pH at the basolateral membrane (pHb) of isolated caecal epithelia of guinea pig using pH-sensitive fluorescein attached to lectin (lens culinaris). In the presence of bicarbonate and a perfusion solution-pH of 7.4, pHb was 7.70 +/- 0.15. In the absence of bicarbonate or chloride as well as by inhibition of the basolateral Cl--HCO-3 exchange with H2-DIDS, pHb was reduced near to solution-pH. Inhibition of the basolateral Na+-H+ exchanger by adding a sodium- and bicarbonate-free, low-buffered solution increased pHb. Decrease of pH of serosal perfusion solution to 6.4 provoked a similar decrease of pHb to solution pH. Short-chain fatty acids (SCFA) added to the mucosal solution caused a slight decrease of pHb. SCFA added to the serosal side alkalized pHb. However, in the presence of bicarbonate pHb returned quickly to the initial pHb, and after removal of SCFA a transient acidification of pHb was seen. These responses could not be inhibited by MIA or H2-DIDS. We conclude that no constant pH-microclimate exists at the basolateral side. The regulation of the intracellular pH of enterocytes reflects pHb. The slightly alkaline pHb is due to the bicarbonate efflux. Data support the presence of an SCFA--HCO-3 exchange.
authors.affiliation: Department of Physiology, School of Veterinary Medicine, D-30173 Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kirschberger
authors.forename: R
authors.initials: R
authors.lastname: Busche
authors.forename: W
authors.initials: W
authors.lastname: von Engelhardt
mesh_terms: D000818:Animals; D002432:Cecum / Q000502:physiology*; D004594:Electrophysiology; D004848:Epithelium; D005232:Fatty Acids, Volatile / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000502:physiology*; D037102:Lectins; D008297:Male; D010477:Perfusion; D037121:Plant Lectins; D012996:Solutions
chemical_list: D005232:Fatty Acids, Volatile; D005456:Fluorescent Dyes; D037102:Lectins; D037121:Plant Lectins; D012996:Solutions; C033619:lentil lectin; D016650:Fluorescein-5-isothiocyanate