Stabilization of polymer-based gene delivery systems.
To preserve the size and transfection potential of polymer-plasmid complexes. Freeze-drying and freeze-thawing were used for stabilization of these complexes. The concentration of the sugars is an important factor affecting both the size and transfection capability of the complexes after freeze-drying and freeze-thawing. However, the type of lyoprotectant (sugar) used is of minor importance. It is also shown that when damage to polymer-plasmid complexes occurs, it results from the drying process but is not due to the freezing step.
Cherng
J Y
JY
Research School Groningen-Utrecht Institute for Drug Exploration (GUIDE), Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80.082, 3508 TB, Utrecht, The Netherlands.
vd Wetering
P
P
Talsma
H
H
Crommelin
D J
DJ
Hennink
W E
WE
D005612:Freeze Drying; D015316:Genetic Therapy; D008320:Maltose / Q000494:pharmacology; D010957:Plasmids; D011108:Polymers; D013395:Sucrose / Q000494:pharmacology; D014162:Transfection; D014199:Trehalose / Q000494:pharmacology
D011108:Polymers; D013395:Sucrose; D008320:Maltose; D014199:Trehalose