Distribution of the rubredoxin gene among the Clostridium butyricum species.
With PCR methods, the rubredoxin gene was systematically identified among 11 strains of Clostridium butyricum; this ubiquity means major functions in the metabolism of the Clostridia. The 11 PCR products allowed deduction of a sequence of 26 amino acids corresponding to positions 11-36 of the rubredoxin. They all contained the tyrosines at positions 11 and 13 and the phenylalanine at position 30 characteristic of the rubredoxin, but differed at positions 14-17, 20, 25, 29, and 31, allowing determination of three types of rubredoxins among these 11 strains of C. butyricum.
Gérard
P
P
Laboratoire de biochimie des bactéries Gram+, Domaine scientifique Victor Grignard, Université Henri Poincaré, Faculté des sciences, Vandoeuvre-les-Nancy, France.
Amine
J
J
Raval
G
G
Petitdemange
H
H
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012416:Rubredoxins / Q000235:genetics*
D012416:Rubredoxins