Cloning and sequencing of the fcbB gene encoding 4-chlorobenzoate-coenzyme A dehalogenase from Pseudomonas sp. DJ-12.
Pseudomonas sp. DJ-12 degrades 4-chlorobenzoate through hydrolytic dechlorination to produce 4-hydroxybenzoate and a chloride ion. The fcbB gene encoding the 4-chlorobenzoate-coenzyme A (4CBA-CoA) dehalogenase which catalyzes the nucleophilic substitution reaction to convert 4CBA-CoA to 4-hydroxybenzoate-coenzyme A (4HBA-CoA) in the consecutive steps of dechlorination was cloned from the chromosome of the organism. A nucleotide sequence analysis of the gene showed an open reading frame consisting of 810 nucleotides, which can encode for a polypeptide of molecular mass 30 kDa, containing 269 amino acid residues. A promoter-like sequence (-35 and -10 region) and a putative ribosome-binding sequence were identified. A deduced amino acid sequence of the 4CBA-CoA dehalogenase showed 86%, 50%, and 50% identity with those of corresponding enzymes in the Pseudomonas sp. CBS3, Arthrobacter sp. SU, and Arthrobacter sp. TM1, respectively.
Chae
J C
JC
Department of Microbiology, Chungbuk National University, Cheongju, Korea.
Kim
Y
Y
Min
K H
KH
Kim
Y C
YC
Kim
C K
CK
D000595:Amino Acid Sequence; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002712:Chlorides / Q000378:metabolism; D002723:Chlorobenzoates / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D010226:Parabens / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002712:Chlorides; D002723:Chlorobenzoates; D004269:DNA, Bacterial; D010226:Parabens; D006867:Hydrolases; C084333:4-chlorobenzoyl coenzyme A dehalogenase; C040768:4-chlorobenzoic acid; C038193:4-hydroxybenzoic acid