High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat.
The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)-malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100 degrees C for 150 min and the DETBA-MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol.
Sakai
T
T
Faculty of Agriculture, Miyazaki University, Japan. a0b102u@cc.miyazaki-u.ac.jp
Habiro
A
A
Kawahara
S
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013858:Thiobarbiturates / Q000737:chemistry*
D013858:Thiobarbiturates; D008315:Malondialdehyde; C046058:1,3-diethyl-2-thiobarbituric acid