title: Expression of estrogen receptor-beta protein in rodent ovary.
abstract: Estrogen is an essential hormone for the LH surge and ovulation. The primary source of estrogen is from ovarian granulosa cells and in rats, estrogen, in turn, increases granulosa cell number and enhances FSH-stimulated gene expression in these cells. Thus, rat granulosa cells both respond to and synthesize estrogen. To further elucidate the mechanisms mediating the actions of estrogen in granulosa cells, we have identified and characterized the estrogen receptor-beta (ER-beta) subtype in rodent granulosa cells. ER-beta protein was localized to the nuclei of rat granulosa cells in preantral and antral follicles by immunocytochemistry, coincident with the location of ER-beta messenger RNA (mRNA). Immunoprecipitation and Western blot analysis using ER-beta specific antisera demonstrated a protein of approximately 60 kDa in granulosa cells prepared from PMSG-primed immature mice and estrogen-treated immature rats. Extracts from granulosa cells specifically bound an estrogen response element and the complex was recognized by antisera to ER-beta. A synthetic steroid estrogen radioligand, [125I]-17alpha-iodovinyl-11beta-methoxyestradiol ([125I]-VME2), bound to cytosolic granulosa cell preparations with high affinity (estimated K(D) value of 401 +/- 83 pM, and Bmax value of 102 +/- 9 fmol/mg protein). ER-beta protein levels rapidly declined following hCG treatment consistent with the reported decrease in binding activity and ER-beta mRNA levels by high levels of gonadotropins. Overall, we have demonstrated that 1) ER-beta protein is the dominant estrogen receptor subtype present in rodent granulosa cells, 2) this receptor is functional, and 3) it is regulated by ovulatory doses of gonadotropins. Thus, ER-beta is likely to be a mediator of estrogen action in rodent granulosa cells during follicular development.
authors.affiliation: Molecular Biology Division, Women's Health Research Institute, Wyeth-Ayerst Research, Radnor, Pennsylvania 19087, USA. fitzpas2@war.wyeth.com
authors.forename: S L
authors.initials: SL
authors.lastname: Fitzpatrick
authors.forename: J M
authors.initials: JM
authors.lastname: Funkhouser
authors.forename: D M
authors.initials: DM
authors.lastname: Sindoni
authors.forename: P E
authors.initials: PE
authors.lastname: Stevis
authors.forename: D C
authors.initials: DC
authors.lastname: Deecher
authors.forename: A R
authors.initials: AR
authors.lastname: Bapat
authors.forename: I
authors.initials: I
authors.lastname: Merchenthaler
authors.forename: D E
authors.initials: DE
authors.lastname: Frail
mesh_terms: D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism; D047629:Estrogen Receptor beta; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D010053:Ovary / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000032:analysis* / Q000235:genetics
chemical_list: D006063:Chorionic Gonadotropin; D047629:Estrogen Receptor beta; D007106:Immune Sera; D011960:Receptors, Estrogen; D004958:Estradiol