title: Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia.
abstract: BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution.
authors.affiliation: Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194, Japan. siwast@libra.ls.m-kagaku.co.jp
authors.forename: S
authors.initials: S
authors.lastname: Iwashita
authors.forename: T
authors.initials: T
authors.lastname: Nobukuni
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Iwanaga
authors.forename: H B
authors.initials: HB
authors.lastname: Tamate
authors.forename: T
authors.initials: T
authors.lastname: Masui
authors.forename: I
authors.initials: I
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000737:chemistry; D003670:Deer; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D011233:Precipitin Tests; D012091:Repetitive Sequences, Nucleic Acid; D012418:Ruminants / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: C104422:CFDP1 protein, human; D009687:Nuclear Proteins; D010750:Phosphoproteins; D004247:DNA