Relationship between morphological changes and endotoxin release induced by carbapenems in Pseudomonas aeruginosa.
The relationship between morphological changes and endotoxin release induced in vitro by carbapenems in a clinical isolate of Pseudomonas aeruginosa was examined. The time-course and magnitude of endotoxin release induced varied among imipenem, panipenem, meropenem and biapenem and related to the morphological changes caused by these agents which variously affected cell shape, cell-wall disintegration and cell lysis. The amount of endotoxin released by carbapenem-treated cells correlated with both the cell-wall morphology and bacterial shape immediately before lysis. Meropenem and biapenem caused markedly increased endotoxin release during cell lysis and cell-wall disintegration, whereas imipenem and panipenem caused much less release of endotoxin.
Horii
Toshinobu
T
Departments of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466.
Ichiyama
Satoshi
S
Department of Clinical Laboratory Medicine, Kyoto University Hospital, 54 Kawahara-cho, Sakyo-ku, Kyoto 606, Japan.
Ohta
Michio
M
Departments of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466.
Kobayashi
Miya
M
Anatomy, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466.
D015780:Carbapenems / Q000494:pharmacology*; D004731:Endotoxins / Q000378:metabolism*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D013845:Thienamycins / Q000494:pharmacology
D015780:Carbapenems; D004731:Endotoxins; D013845:Thienamycins; D015378:Imipenem; D000077731:Meropenem; C039536:panipenem; C065257:biapenem