title: Phylogenetic evidence for recombination in dengue virus.
abstract: A split decomposition analysis of dengue (DEN) virus gene sequences revealed extensive networked evolution, indicative of recombination, among DEN-1 strains but not within serotypes DEN-2, DEN-3, or DEN-4. Within DEN-1, two viruses sampled from South America in the last 10 years were identified as recombinants. To map the breakpoints and test their statistical support, we developed a novel maximum likelihood method. In both recombinants, the breakpoints were found to be in similar positions, within the fusion peptide of the envelope protein, demonstrating that a single recombination event occurred prior to the divergence of these two strains. This is the first report of recombination in natural populations of dengue virus.
authors.affiliation: Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, U.K. Edward.Holmes@zoo.ox.ac.uk
authors.forename: E C
authors.initials: EC
authors.lastname: Holmes
authors.forename: M
authors.initials: M
authors.lastname: Worobey
authors.forename: A
authors.initials: A
authors.lastname: Rambaut
mesh_terms: D003715:Dengue / Q000517:prevention & control / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D005814:Genes, Viral; D015752:Genes, env; D006801:Humans; D016013:Likelihood Functions; D010802:Phylogeny; D011995:Recombination, Genetic; D012703:Serotyping; D014760:Viral Fusion Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D014760:Viral Fusion Proteins; D014765:Viral Vaccines