Analysis of the antigenicity in mice of biotinyl enzymes from Anisakis simplex and other nematodes.
We analyzed the antigenicity of biotinyl enzymes contained in somatic extracts from Anisakis simplex and other parasite nematodes and show in this report that these molecules are an important source of cross-reactivity problems among these nematodes. Cross-reactivity was most pronounced among members of the superfamily Ascaridoidea. These results suggest that the presence of biotinyl enzymes in whole somatic extracts of A. simplex and other parasites may make serodiagnostic assays based on this kind of antigenic preparation unreliable.
Lorenzo
S
S
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
Iglesias
R
R
Paniagua
E
E
Leiro
J
J
Ubeira
F M
FM
D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D001710:Biotin; D002262:Carboxy-Lyases / Q000276:immunology*; D002678:Chimera; D003429:Cross Reactions; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009348:Nematoda / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001710:Biotin; D002262:Carboxy-Lyases