title: Separation of enantiomers on a chiral stationary phase based on ovoglycoprotein. V. Influence of immobilization method on chiral resolution.
abstract: Ovoglycoprotein from chicken egg whites (OGCHI) was bound to aminopropyl-silica gels via an amino or carboxyl group(s) of OGCHI. In the former case, OGCHI was bound to N,N'-disuccinimidyl carbonate-activated aminopropyl-silica gels, while in the latter case OGCHI activated by a water-soluble carbodiimide and N-hydroxysulfosuccinimide was bound to aminopropyl-silica gels. The obtained OGCHI materials were compared with regard to the bound amounts, retentivity and enantioselectivity. The OGCHI materials prepared via a carboxyl group(s) of OGCHI are suitable for chiral resolution of acidic solutes, and those via an amino group(s) of OGCHI are suitable for chiral resolution of basic solutes. It is suggested that the electrostatic interaction between an amino or carboxyl group of OGCHI and a charged solute should play an important role in chiral recognition of the solute.
authors.affiliation: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan. haginaka@mwu.mukogawa-u.ac.jp
authors.forename: J
authors.initials: J
authors.lastname: Haginaka
authors.forename: Y
authors.initials: Y
authors.lastname: Okazaki
authors.forename: H
authors.initials: H
authors.lastname: Matsunaga
mesh_terms: D001573:Benzoin / Q000302:isolation & purification; D002744:Chlorpheniramine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004529:Egg White / Q000032:analysis*; D006020:Glycopeptides / Q000302:isolation & purification*; D007052:Ibuprofen / Q000032:analysis; D007202:Indicators and Reagents; D058428:Silica Gel; D012822:Silicon Dioxide; D012996:Solutions; D013237:Stereoisomerism
chemical_list: D006020:Glycopeptides; D007202:Indicators and Reagents; D012996:Solutions; C013176:ovoglycopeptide; D002744:Chlorpheniramine; D058428:Silica Gel; D012822:Silicon Dioxide; D001573:Benzoin; D007052:Ibuprofen