High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey.
Cottage cheese whey is a cheese industry by-product still rich in proteins and lactose. Its recycling is seldom cost-effective. In this work we show that the lactose-utilizing yeast Kluyveromyces lactis, engineered for production of recombinant human lysozyme, can be grown in cottage cheese whey, resulting in high-level production of the heterologous protein (125 microg/ml).
Maullu
C
C
Dipartimento di Scienze Mediche, Cattedra di Microbiologia Applicata, Università di Cagliari, 09124 Cagliari, Italy.
Lampis
G
G
Desogus
A
A
Ingianni
A
A
Rossolini
G M
GM
Pompei
R
R
D002611:Cheese; D003470:Culture Media; D005818:Genetic Engineering; D006801:Humans; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D007785:Lactose / Q000378:metabolism*; D008894:Milk Proteins / Q000378:metabolism*; D009113:Muramidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D000067816:Whey Proteins
D003470:Culture Media; D008894:Milk Proteins; D011994:Recombinant Proteins; D000067816:Whey Proteins; D009113:Muramidase; D007785:Lactose