Differential inhibition of Maillard protein fluorescence by nitric oxide donors.
The nitric oxide donors nitroprusside (NP) and S-nitroso-N-acetylpenicillamine (SNAP) were added repeatedly over a prolonged period into a protein fructation system of 0.05 M fructose and BSA. These additions inhibited Maillard reaction advanced-stage fluorescence generation in a dose-dependent manner without affecting initiation of glycation. NP caused 66% inhibition whereas SNAP caused only 30% inhibition at maximum dose. The lower inhibition by SNAP possibly reflects an interference caused by N-acetylpenicillamine and mediated by a metal-dependent enhanced free-radical generation. We propose that the inhibition of fluorescence results from mutual annihilation between nitric oxide and free radicals, such as OH*, produced during fructation. In vivo generated nitric oxide may play a protective role in cells against the deleterious effect of free radicals that are associated with the augmented fructose autoxidation and fructation that occurs in diabetes.
Suárez
G
G
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA.
Wang
X H
XH
D005453:Fluorescence; D005947:Glucose / Q000737:chemistry; D015416:Maillard Reaction; D020030:Nitric Oxide Donors / Q000737:chemistry*; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000737:chemistry; D017382:Reactive Oxygen Species; D026423:S-Nitroso-N-Acetylpenicillamine; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D020030:Nitric Oxide Donors; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D026423:S-Nitroso-N-Acetylpenicillamine; D010396:Penicillamine; D005947:Glucose