Biodiversity in deep-sea sites located near the south part of Japan.
We obtained 100 isolates of bacteria from deep-sea mud samples collected at various depths (1050-10897m). Various types of bacteria such as alkaliphiles, thermophiles, psychrophiles, and halophiles were recovered on agar plates at a frequency of 0.8 x 10(2) to 2.3 x 10(4)/ g of dry sea mud. No acidophiles were recovered. These extremophilic bacteria were widely distributed, being detected at each deep-sea site, and the frequency of isolation of such extremophiles from the deep-sea mud was not directly influenced by the depth of the sampling sites. Phylogenetic analysis of deep-sea isolates based on 16S rDNA sequences revealed that a wide range of taxa were represented in the deep-sea environments. Growth patterns under high hydrostatic pressure were determined for the deep-sea isolates obtained in this study. No extremophilic strains isolated in this study showed growth at 60MPa, although a few of the other isolates grew slightly at this hydrostatic pressure.
Takami
H
H
Deep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, Yokosuka. takamih@jamstec.go.jp
Kobata
K
K
Nagahama
T
T
Kobayashi
H
H
Inoue
A
A
Horikoshi
K
K
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial; D004275:DNA, Ribosomal / Q000032:analysis; D007564:Japan; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D011312:Pressure; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S