Human autoantibodies identify a protein in dense fibrillar and granular components of the nucleolus.
A novel nucleolar protein of an approximate molecular weight of 60 kDa was identified by immunoprecipitation in human cells with an autoimmune sclerodermic serum. It maps at the ultrastructural level to nucleolar granular and dense fibrillar components. This 60 kDa protein could not be demonstrated in Western blots suggesting that the epitope structure is complex and/or is sensitive to the treatment of cells. The immunoprecipitation results indicate that the 60 kDa protein is not a phosphoprotein and is not associated with a nucleolar RNA containing particle. The identified protein represents a new autoimmune marker in the field of systemic connective tissue diseases.
Raska
I
I
Department of Cell Biology, Academy of Sciences of the Czech Republic, Prague.
Reimer
G
G
D001323:Autoantibodies / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D003609:Dactinomycin / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011233:Precipitin Tests; D013997:Time Factors
D001323:Autoantibodies; D019384:Nucleic Acid Synthesis Inhibitors; D003609:Dactinomycin; D004004:Dichlororibofuranosylbenzimidazole