Direct visual resolution of gene copy number in the human photopigment gene array.
OBJECTIVE
To visualize by direct fluorescent in situ hybridization the entire human visual pigment gene array on single X-chromosome fibers and to compare the results with values obtained by other molecular techniques.


METHODS
The size of the opsin gene array on the X-chromosome in eight male subjects was investigated by (i) direct visual in situ hybridization (DIRVISH) on elongated DNA fibers: (ii) quantitation of genomic restriction fragments after Southern blot hybridization; (iii) quantitation of restriction fragment length polymorphism after PCR amplification (PCR/RFLP), and (iv) sizing of NotI fragments by pulsed field gel electrophoresis and Southern blot detection. Each male subject's color vision was assessed by Rayleigh matches on a Nagel Type 1 anomaloscope.


RESULTS
The number of genes resolved by the DIRVISH protocol, which ranges from 1 to 6, agrees exactly with the gene array sizes obtained in the same male subjects from pulsed field gel electrophoresis, but differs from the estimates derived from the commonly used indirect Southern blot hybridization and PCR/RFLP quantitation methods. In particular, the PCR/RFLP method overestimates the copy number in all but the smallest arrays.


CONCLUSIONS
Visualization of the X-chromosome opsin gene array by DIRVISH provides a new, direct method for obtaining exact copy numbers and helps to resolve the controversy about the range and the average visual pigment gene number in the human population in favor of smaller average array sizes.
Wolf
S
S
Molekulargenetisches Labor, Universitäts-Augenklinik, Tübingen, Germany.
Sharpe
L T
LT
Schmidt
H J
HJ
Knau
H
H
Weitz
S
S
Kioschis
P
P
Poustka
A
A
Zrenner
E
E
Lichter
P
P
Wissinger
B
B
D015139:Blotting, Southern; D004247:DNA / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017299:Rod Opsins / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics*
D017299:Rod Opsins; D004247:DNA