title: Predictive value of malignancy grading systems, DNA content, p53, and angiogenesis for stage I tongue carcinomas.
abstract: OBJECTIVE
  To assess the clinical value of malignancy grading systems compared with nuclear DNA content, protein p53, and angiogenesis for predicting recurrence of stage I (UICC, 1987) tongue carcinomas.
  
  
  METHODS
  Histopathological malignancy grading according to Jakobsson and tumour front grading according to Bryne et al were performed on haematoxylin and eosin slides. DNA analysis was performed by image cytometry. Protein p53 and angiogenesis were evaluated by immunohistochemical analysis using antibody CM1 and antibody against factor VIII related antigen, respectively.
  
  
  RESULTS
  49 patients with stage I carcinomas of the mobile tongue were included, all treated by local surgical excision alone. Eight patients (16%) suffered from local recurrence during follow up, and 13 (27%) had regional recurrence. Both Jakobsson's malignancy grading system and p53 immunoreactivity proved to be useful predictors of regional recurrence in a Cox multivariate regression analysis.
  
  
  CONCLUSIONS
  Histopathological malignancy grading systems provide valuable prognostic information and can still compete with current biological markers in this respect.
authors.affiliation: Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Karolinska Hospital, Stockholm, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Högmo
authors.forename: R
authors.initials: R
authors.lastname: Kuylenstierna
authors.forename: J
authors.initials: J
authors.lastname: Lindholm
authors.forename: E
authors.initials: E
authors.lastname: Munck-Wikland
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000473:pathology* / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011379:Prognosis; D012008:Recurrence; D014062:Tongue Neoplasms / Q000098:blood supply / Q000473:pathology* / Q000601:surgery; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53