Mucin production in the middle ear in response to lipopolysaccharides.
OBJECTIVE
This study examined the response of middle ear tissue to establish the lowest dose of lipopolysaccharide to induce mucin production in a rat otitis media model.


METHODS
Twenty-six male Sprague-Dawley rats' eustachian tubes were obstructed before transtympanic inoculation of the bulla tympanica with 35 microL of Krebs Ringer or 1, 10, 100, or 1000 microgram/mL lipopolysaccharide. After 7 days the effusion and a lavage were collected for mucin ELISA measurement, and tissue was collected for histologic evaluation.


RESULTS
Mucin secretion was significantly increased in the 100 microgram/mL 51.20 +/- 13.6 microgram/mL (SE) and 1000 microgram/mL 69.42 +/- 8.57 microgram/mL groups when compared with the Krebs Ringer control group 1.84 +/- 0.28 microgram/mL (P < 0.05). Histologic evaluation shows goblet cell metaplasia and hyperplasia in the middle ear epithelium in the 1000 and 100 microgram/mL groups.


CONCLUSIONS
The histology and ELISA results suggest that a middle ear effusion is generated with a dose of lipopolysaccharide as low as 100 microgram/mL.
Hunter
S E
SE
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of North Carolina School of Medicine, Chapel Hill, USA.
Singla
A K
AK
Prazma
J
J
Jewett
B S
BS
Randell
S H
SH
Pillsbury
H C
HC
D000818:Animals; D004195:Disease Models, Animal; D004432:Ear, Middle / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009092:Mucous Membrane / Q000378:metabolism; D010034:Otitis Media with Effusion / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; D009077:Mucins