Reduced blood bcl-2 protein concentration in patients on hemodialysis.
The concentrations of bcl-2 protein that can block programmed cellular death in various cell lines were evaluated in blood samples from 10 uremic patients on hemodialysis, 10 uremics not yet on hemodialysis, and in 10 healthy controls. The bcl-2 protein variations (in uremics on dialysis) were ascertained in patients during the dialysis session. Oxidative stress was evaluated in all groups by assaying the products of intraerythrocytic lipoperoxidation. Dialyzed and nondialyzed uremic patients had higher bcl-2 protein concentrations than healthy subjects. Dialysis causes a significant reduction in the concentrations of bcl-2 protein which becomes statistically significant during the 3rd hour. In both groups of uremic patients a positive correlation was found between bcl-2 protein and products of lipoperoxidation.
Buemi
M
M
Cattedra di Nefrologia II, Dipartimento di Medicina Interna, Policlinico Universitario di Messina, Italia.
Allegra
A
A
Corica
F
F
Ruello
M
M
Di Pasquale
G
G
Aloisi
C
C
Giaccobe
M S
MS
Romeo
A
A
Frisina
N
N
D000368:Aged; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D004912:Erythrocytes / Q000737:chemistry; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D007958:Leukocyte Count; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood / Q000172:deficiency*; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood* / Q000628:therapy
D019253:Proto-Oncogene Proteins c-bcl-2; D004921:Erythropoietin