[Detection of p53 and MDM2 gene expression in osteosarcoma with biotin-labelled in situ].
We studied the mRNA expression of p53 and MDM2 gene in osteosarcoma. Twenty osteosarcomas were examined with biotin-labelled oligodeoxynucleotide probe in situ. Of 20 tumors, 8 (40%) and 6(30%) showed positive expression of p53 and MDM2 genes, respectively. Among these, 2 tumors revealed co-expression of both p53 and MDM2 genes. This study confirmed the existence of aberrant mRNA expression of p53 and MDM2 genes in osteosarcoma.
Wang
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Bone Tumor Research Laboratory, People's Hospital, Beijing Medical University.
Wang
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Feng
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D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism; D002648:Child; D005260:Female; D005266:Femoral Neoplasms / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051736:Proto-Oncogene Proteins c-mdm2; D012333:RNA, Messenger / Q000096:biosynthesis; D013977:Tibia
D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2