Inhibitory effect of mast cell-mediated immediate-type allergic reactions by Cichorium intybus.
We investigated the effect of an aqueous extract of Cichorium intybus (CIAE) on mast cell-mediated immediate type allergic reactions. CIAE (0.1-1000 mg kg-1) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. Especially, CIAE inhibited compound 48/80-induced anaphylactic reaction 100% with the dose of 1000 mg kg-1. CIAE 1000 mg kg-1also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with CIAE at a concentration ranging from 0.1 to 1000 mg kg-1, the plasma histamine levels were reduced in a dose-dependent manner. CIAE (1-1000 microg ml-1) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMC, when CIAE (1000 microg ml-1) was added, increased significantly compared with that of control cells. These results indicate that CIAE inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.
Kim
H M
HM
College of Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, South Korea.
Kim
H W
HW
Lyu
Y S
YS
Won
J H
JH
Kim
D K
DK
Lee
Y M
YM
Morii
E
E
Jippo
T
T
Kitamura
Y
Y
An
N H
NH
D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control; D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D018651:Cichorium intybus / Q000737:chemistry / Q000627:therapeutic use*; D000242:Cyclic AMP / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000188:drug therapy*; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine
D018926:Anti-Allergic Agents; D010936:Plant Extracts; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP