Determination of the relative impact of chromatographic conditions and solute structures on the retention behaviour of non-ionic surfactants in RP-HPLC.
The retention behaviour of non-ionic surfactants containing different hydrophobic moiety and various lengths of polar ethylene oxide chain was studied on RP-8 and RP-18 columns using methanol and acetonitrile as organic modifiers. The relative impact of the chromatographic conditions on the retention strength was determined using stepwise regression analysis. Non-ionic surfactants can be separated according to the character of the hydrophobic moiety, but not according to the length of the polar ethylene oxide chain. Calculations proved that the organic modifier exerted the highest impact on the retention; the effect of the number of carbon atoms in the hydrophobic moiety, column temperature and the length of the hydrophobic ligand on the surface of silica support are of secondary importance.
Balcan
M
M
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
Cserháti
T
T
Forgács
E
E
Anghel
D F
DF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D012044:Regression Analysis; D013501:Surface-Active Agents / Q000737:chemistry*
D013501:Surface-Active Agents