Preliminary investigation of resistance of plasmid DNA to neon ion beams using transformation into recipient Escherichia coli cells.
The sensitivity of the vector plasmid pKmK8 DNA to neon ion beams was studied under dry conditions. This plasmid contains the cloning vector pJKKmf(-) and a 3.6 kbp segment encoding the Escherichia coli crp gene that can be used in mutagenesis analysis. The survival curve of the plasmid indicated an exponential profile and a D10 value of about 16 kGy in the E. coli wild-type strain for DNA repair capability. This was similar to the D10 value for the shuttle vector plasmid pZ189 DNA. Therefore, it was assumed that the excision repair system in E. coli was not effective for repairing DNA lesions induced by irradiation with heavy ion beams.
Imamura
M
M
The Third Department of Internal Medicine, Kansai Medical University, Moriguchi-shi, Osaka 570-8506, Japan.
Seki
T
T
Tamai
T
T
Nakagawa
T
T
Nishimura
A
A
Yamashiki
N
N
Inoue
K
K
Akagi
K
K
Sawada
S
S
Kasahara-Imamura
M
M
Okamura
A
A
Takimoto
K
K
Watanabe
H
H
Ohnishi
T
T
Harada
K
K
D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects*; D005798:Genes, Bacterial; D009356:Neon; D010957:Plasmids / Q000235:genetics*; D014170:Transformation, Genetic / Q000528:radiation effects*
D009356:Neon