Use of recombinant human cardiac Troponin T for standardization of third generation Troponin T methods.
The Elecsys Troponin T third generation assay uses recombinant human cardiac troponin T as standard material. The new assay has a linear calibration curve. Thus, linearity problems observed with the second generation assay have been eliminated. The assay has high precision, especially at the low end of measuring range (inter-assay CV < 10% at 0.1 microgram/L). The new standardization does not change the cut-off value of 0.1 microgram/L. The use of recombinant human cardiac troponin T as standard material enables a reproducible and reliable standardization of troponin T assays.
Hallermayer
K
K
Roche Diagnostics GmbH, Werk Penzberg, Germany.
Klenner
D
D
Vogel
R
R
D015415:Biomarkers; D002138:Calibration; D002624:Chemistry, Clinical / Q000592:standards*; D003327:Coronary Disease / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016014:Linear Models; D011994:Recombinant Proteins; D012016:Reference Values; D015203:Reproducibility of Results; D020107:Troponin T / Q000032:analysis / Q000097:blood
D015415:Biomarkers; D011994:Recombinant Proteins; D020107:Troponin T