Regulation of inner ear fluid in the rat by vasopressin.
The anti-diuretic hormone vasopressin has been shown to be important in regulating inner ear fluid. The diuretic hormone, CNP, and its receptor, ANP-B receptor, may also function in the regulation of inner ear fluid. To determine whether vasopressin directly affects the fluid level, we infused this hormone to rat and assay of V2-AVP receptor mRNA by semiquantitative RT-PCR demonstrated a significantly lower level of this transcript in vasopressin-infused animals than in saline-infused animals. The levels of CNP and ANP-B receptors mRNA, however, were the same in both groups of rats. Results suggest that high plasma levels of vasopressin may be a principal causal factor of endolymphatic hydrops in Meniere's disease, perhaps by down-regulating the number of vasopressin receptors.
Kitano
H
H
Department of Otolaryngology, Shiga University of Medical Science, Otsu, Japan.
Suzuki
M
M
Kitanishi
T
T
Yazawa
Y
Y
Kitajima
K
K
Isono
T
T
Takeda
T
T
Kimura
H
H
Tooyama
I
I
D000818:Animals; D001127:Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology*; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology*; D004710:Endolymph / Q000502:physiology; D006162:Guanylate Cyclase / Q000235:genetics; D006706:Homeostasis; D007263:Infusions, Parenteral; D007761:Labyrinthine Fluids / Q000187:drug effects / Q000502:physiology*; D008575:Meniere Disease / Q000209:etiology / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics; D017483:Receptors, Vasopressin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D000099242:Atrial Natriuretic Factor Receptor B
D001127:Arginine Vasopressin; D006162:Guanylate Cyclase; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; D017483:Receptors, Vasopressin; D000099242:Atrial Natriuretic Factor Receptor B; C073616:atrial natriuretic factor receptor C