title: Invasiveness of Helicobacter pylori into human gastric mucosa.
abstract: BACKGROUND
  Helicobacter pylori has generally been observed only in the gastric mucous layer or in the spaces between gastric mucus-secreting cells and not in the gastric epithelial cells or in the lamina propria. The purpose of this study is to determine whether H. pylori invades the gastric mucosa, using an immunoelectron microscopical examination of human gastric mucosa infected with H. pylori.
  
  
  METHODS
  Five hundred gastric antral biopsy specimens were fixed in a periodate-lysin-paraformaldehyde solution, embedded in Lowicryl, sectioned, and examined with a light microscope. One hundred specimens moderately or severely infected with H. pylori were selected and were incubated with polyclonal rabbit anti-H. pylori antibody. The specimens were washed, incubated with 20 nm of colloidal gold-conjugated goat anti-rabbit IgG, stained with uranyl acetate and lead citrate, and observed with a transmission electron microscope.
  
  
  RESULTS
  In one case, a bacterium was observed within the cytoplasm of a gastric mucus-secreting cell; in another case, a few bacteria were observed within the cytoplasm of a stromal cell in the lamina propria. The bacteria could be differentiated from degenerated intracellular organelles by gold particles attached to the bacteria.
  
  
  CONCLUSIONS
  H. pylori rarely invade the lamina propria and gastric cells.
authors.affiliation: Department of Pathology, Gyeongsang National University College of Medicine, Chinju, Korea. gyunghko@gshp.gsnu.ac.kr
authors.forename: G H
authors.initials: GH
authors.lastname: Ko
authors.forename: S M
authors.initials: SM
authors.lastname: Kang
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: C K
authors.initials: CK
authors.lastname: Park
authors.forename: H S
authors.initials: HS
authors.lastname: Youn
authors.forename: S C
authors.initials: SC
authors.lastname: Baik
authors.forename: M J
authors.initials: MJ
authors.lastname: Cho
authors.forename: W K
authors.initials: WK
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000328:Adult; D001706:Biopsy; D005753:Gastric Mucosa / Q000382:microbiology* / Q000648:ultrastructure; D016480:Helicobacter pylori / Q000472:pathogenicity* / Q000648:ultrastructure; D006801:Humans; D016253:Microscopy, Immunoelectron; D011706:Pyloric Antrum / Q000382:microbiology* / Q000648:ultrastructure