Intracerebroventricularly injected L-arginine-induced vasopressin release is mediated by cGMP in rats.
OBJECTIVE
To study the possible role of cGMP in the effect of L-arginine-induced arginine-vasopressin (AVP) release.


METHODS
In anesthetized rats, saline, L-arginine, 8-Br-cGMP, L-arginine + methylene blue (an inhibitor of guanylyl cyclase) were injected intracerebroventricularly (icv) and the plasma vasopressin level was detected with radioimmunoassay.


RESULTS
Both 8-Br-cGMP 2.53 g.L-1 and L-arginine 100 g.L-1 increased plasma AVP level [from (2.6 +/- 0.3) to (6.6 +/- 0.4) ng.L-1, and from (3.2 +/- 0.5) to (5.8 +/- 1.4) ng.L-1, respectively; P < 0.01] 5 min after the icv injection; methylene blue (3.74 g.L-1) + L-arginine (100 g.L-1) did not change plasma AVP level.


CONCLUSIONS
cGMP was a mediator of the effect of L-arginine-induced AVP release.
Cao
L X
LX
Shanghai Brain Research Institute, Chinese Academy of Sciences, China.
Shen
E
E
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D001127:Arginine Vasopressin / Q000097:blood*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000502:physiology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D007276:Injections, Intraventricular; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D001127:Arginine Vasopressin; C016276:8-bromocyclic GMP; D001120:Arginine; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D008751:Methylene Blue