title: Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results.
abstract: Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a significantly higher plasma level of MPO in horses operated for strangulation obstruction of the large intestine (n = 6) than in horses suffering from a non-strangulating displacement of the large intestine (n = 9). For the 2 groups, 3 phases were distinguished: reception (P1), intensive care (P2) and terminal phase (P3). The mean peak values of MPO for these phases were 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3) for the non-strangulating group, and 242.6 ng/mL (P1); 426.0 ng/mL (P2), and 379.5 ng/mL (P3) for the strangulation group. The variations of the mean peak values of plasma MPO were significantly different between the 2 groups and between the different phases. A significant increase of the least square means of MPO was observed between P1 and P2. A significant decrease of the least square means of the number of circulating leukocytes was observed between P1 and P3. Polymorphonuclear neutrophil activation could play a major role in the pathogenesis of acute abdominal disease and endotoxic shock.
authors.affiliation: Anaesthésiologie générale, Pathologie chirugicale des grands animaux, Université de Liège, Sart Tilman, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Grulke
authors.forename: H
authors.initials: H
authors.lastname: Benbarek
authors.forename: I
authors.initials: I
authors.lastname: Caudron
authors.forename: G
authors.initials: G
authors.lastname: Deby-Dupont
authors.forename: M
authors.initials: M
authors.lastname: Mathy-Hartert
authors.forename: F
authors.initials: F
authors.lastname: Farnir
authors.forename: C
authors.initials: C
authors.lastname: Deby
authors.forename: M
authors.initials: M
authors.lastname: Lamy
authors.forename: D
authors.initials: D
authors.lastname: Serteyn
mesh_terms: D000208:Acute Disease; D000818:Animals; D003108:Colonic Diseases / Q000503:physiopathology / Q000601:surgery / Q000662:veterinary*; D003422:Critical Care; D006734:Horse Diseases / Q000097:blood* / Q000503:physiopathology / Q000601:surgery; D006736:Horses; D007415:Intestinal Obstruction / Q000097:blood* / Q000503:physiopathology / Q000601:surgery*; D016018:Least-Squares Analysis; D007958:Leukocyte Count; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000097:blood*
chemical_list: D009195:Peroxidase