title: The development of resistance to caffeine in Drosophila prosaltans: productivity and longevity after ten generations of treatment.
abstract: The productivity of Drosophila prosaltans treated with six concentrations of caffeine (from 50 micrograms/ml to 2,500 micrograms/ml of culture medium) during ten generations (approximately 8 months) decreased in a dosage dependent manner in every generation, but at the end of the treatment the flies in all experiments recovered normal productivity, except for those treated with 2,500 micrograms/ml. Longevity in the tenth generation was significantly reduced in males and females only in the 2,500 micrograms/ml dosage, with males being much more affected than females. In a previous study in which the treatment was done in a single generation, productivity exhibited only a partial recovery when the treatment ceased and longevity was significantly reduced in 1,500 micrograms/ml dosages. The hypothesis of selection occurring in ten generations leading to recovery in productivity and to a reduction in the processes which cause a decrease in longevity is being considered.
authors.affiliation: Departamento de Biologia, Universidade Estadual Paulista Júlio de Mesquita Filho-UNESP, Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Itoyama
authors.forename: H E
authors.initials: HE
authors.lastname: Bicudo
authors.forename: A J
authors.initials: AJ
authors.lastname: Manzato
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004330:Drosophila / Q000187:drug effects / Q000254:growth & development*; D005260:Female; D005298:Fertility / Q000187:drug effects; D008136:Longevity / Q000187:drug effects*; D008297:Male; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*
chemical_list: D010726:Phosphodiesterase Inhibitors; D002110:Caffeine