Cell cycle arrest in endometrial carcinoma cells exposed to gonadotropin-releasing hormone analog.
Gonadotropin-releasing hormone (GnRH) has been shown to have an inhibitory effect on the growth of several hormone-dependent human tumors. We have treated a human endometrial cancer cell line which expresses GnRH receptor with GnRH analog, D-Trp6-LHRH, in order to study whether there are differences in cell cycle kinetic response. Flow cytometric analysis revealed that cultured carcinoma cells showed a cell cycle arrest at the G1-S transition after treatment with 10 microM D-Trp6-LHRH for 36 h. Western blot analysis showed that the level of p16 protein was obvious following 24 h of D-Trp6-LHRH treatment. These results suggest that the mechanism by which GnRH inhibits the growth of endometrial carcinoma cells may include effects on cell cycle arrest.
Kim
J W
JW
Kangnam St. Mary's Hospital, Catholic Research Institutes of Medical Science, Catholic University Medical College, 505 Banpo-dong, Seocho-ku, Seoul, 137-040, Korea.
Lee
Y S
YS
Kim
B K
BK
Park
D C
DC
Lee
J M
JM
Kim
I K
IK
Namkoong
S E
SE
D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D017329:Triptorelin Pamoate; D014407:Tumor Cells, Cultured
D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone