title: The effects of selenium deficiency on differentiation, degradation, and cell lysis of L8 rat skeletal muscle cells.
abstract: We investigated the effects of selenium (Se) deficiency on differentiation, protein degradation, and cell lysis in cultured skeletal muscle cells, using L8 rat skeletal muscle cells cultured in serum-free (SF) medium to induce differentiation and to maintain myotubes. Creatine kinase activity was reduced (p < 0.05) by approximately 15% without Se supplementation for 96 h. Confluent myoblasts were treated with SF media with four different levels of vitamin E (0, 10, 35, and 100 microM) in the absence and presence of Se (0 and 0.25 microM, respectively). After 96 h, vitamin E at a high dose (100 microM) was effective in the prevention of the decrease of differentiation caused by Se deficiency (p < 0.05). Following differentiation, the effects of three Se concentrations (0, 0.25, and 2.5 microM) on degradation of proteins as assessed by release of 3H-labeled free amino acids secreted into the media were studied. Selenium supplementation did not affect (p > 0.05) total protein degradation. However, Se deficiency increased (p < 0.05) lactate dehydrogenase released from lyzed dead cells. The results indicate that Se is required to maintain an optimal rate of muscle cell differentiation and health of myotube cultures.
authors.affiliation: Department of Animal Sciences, Oregon State University, Corvallis 97331, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda
authors.forename: P D
authors.initials: PD
authors.lastname: Whanger
authors.forename: N E
authors.initials: NE
authors.lastname: Forsberg
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003402:Creatine Kinase / Q000378:metabolism; D016895:Culture Media, Serum-Free; D005979:Glutathione Peroxidase / Q000378:metabolism; D006868:Hydrolysis; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D016895:Culture Media, Serum-Free; D009124:Muscle Proteins; D014810:Vitamin E; D005979:Glutathione Peroxidase; D003402:Creatine Kinase; D012643:Selenium