Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA.
We found that the Escherichia coli cafA::cat mutant accumulated a precursor of 16S rRNA. This precursor migrated to the same position with 16.3S precursor found in the BUMMER strain that is known to be deficient in the 5' end processing of 16S rRNA. Accumulation of 16. 3S rRNA in the BUMMER mutant was complemented by introduction of a plasmid carrying the cafA gene. The mutant type cafA gene cloned from the BUMMER strain had a 11-bp deletion in its coding region. A small amount of the mature 16S rRNA was still formed in the cafA::cat mutant. This residual activity was found to be due to RNase E encoded by the rne/ams gene by rifampicin-chase experiments of the cafA::cat ams1 double mutant. These results indicated that the cafA gene encodes a novel RNase responsible for processing of the 5' end of 16S rRNA.
Wachi
M
M
Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan. mwachi@bio.titech.ac.jp
Umitsuki
G
G
Shimizu
M
M
Takada
A
A
Nagai
K
K
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010957:Plasmids; D012322:RNA Precursors / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012260:Ribonucleases / Q000235:genetics*; D012293:Rifampin / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012322:RNA Precursors; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004722:Endoribonucleases; D012260:Ribonucleases; C081905:rng protein, E coli; C022537:ribonuclease E; D012293:Rifampin