alpha-Dystroglycan is a laminin receptor involved in extracellular matrix assembly on myotubes and muscle cell viability.
alpha-Dystroglycan (alpha-DG) is a laminin-binding protein and member of a glycoprotein complex associated with dystrophin that has been implicated in the etiology of several muscular dystrophies. To study the function of DG, C2 myoblasts were transfected stably with an antisense DG expression construct. Myotubes from two resulting clones (11F and 11E) had at least a 40-50% and 80-90% reduction, respectively, in alpha-DG but normal or near normal levels of alpha-sarcoglycan, integrin beta1 subunit, acetylcholine receptors (AChRs), and muscle-specific kinase (MuSK) when compared with parental C2 cells or three clones (11A, 9B, and 10C) which went through the same transfection and selection procedures but expressed normal levels of alpha-DG. Antisense DG-expressing myoblasts proliferate at the same rate as parental C2 cells and differentiate into myotubes, however, a gradual loss of cells was observed in these cultures. This loss correlates with increased apoptosis as indicated by greater numbers of nuclei with condensed chromatin and more nuclei labeled by the TUNEL method. Moreover, there was no sign of increased membrane permeability to Trypan blue as would be expected with necrosis. Unlike parental C2 myotubes, 11F and 11E myotubes had very little laminin (LN) on their surfaces; LN instead tended to accumulate on the substratum between myotubes. Exogenous LN bound to C2 myotubes and was redistributed into plaques along with alpha-DG on their surfaces but far fewer LN/alpha-DG plaques were seen after LN addition to 11F or 11E myotubes. These results suggest that alpha-DG is a functional LN receptor in situ which is required for deposition of LN on the cell and, further, implicate alpha-DG in the maintenance of myotube viability.
Montanaro
F
F
Center for Research in Neuroscience, McGill University, Montreal General Hospital Research Institute, Montreal, Quebec H3G 1A4, Canada.
Lindenbaum
M
M
Carbonetto
S
S
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002455:Cell Division; D002459:Cell Fusion; D002462:Cell Membrane / Q000378:metabolism; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D049030:Dystroglycans; D005109:Extracellular Matrix / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D011485:Protein Binding; D016372:RNA, Antisense / Q000235:genetics / Q000502:physiology; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D049031:Sarcoglycans; D014162:Transfection
D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D016023:Integrins; D007797:Laminin; D008562:Membrane Glycoproteins; D016372:RNA, Antisense; D011950:Receptors, Cholinergic; D017474:Receptors, Laminin; D049031:Sarcoglycans; D049030:Dystroglycans; C095714:MUSK protein, human; D020794:Receptor Protein-Tyrosine Kinases