Characteristic transfer of colostral components into cerebrospinal fluid via serum in neonatal pigs.
In order to evaluate the possibility of modification of brain function by colostral suckling, the characteristic transfer of colostral components into serum and cerebrospinal fluid (CSF) has been studied by SDS electrophoresis, immunoblot and ELISA methods in nonsuckling pigs. Total protein concentrations in the serum increased immediately after oral administration of bovine colostrum, reaching a peak value (7.0 +/- 0.7 g/dl) at 24 h after administration, corresponding to a 3-fold increase compared to preinfusion levels. IgG and other macromolecular components (MW 19, 000-58,000) were recognized in serum by electrophoretic and ELISA analysis. Total protein concentrations in the CSF collected from the cisterna magna also increased steeply after colostral administration, reaching a maximal value (54.1 +/- 5.0 mg/dl) at 4 h, corresponding to a 4-fold increase compared to preinfusion levels. Two colostral components (MW 19,000 and 31,000) in serum were confirmed to be present in the CSF by electrophoresis. The component of MW 19,000 was identified by immunoblot as beta-lactoglobulin. IgG in serum transferred from colostrum could not be detected in the CSF by ELISA. Lactoferrin administered into the intestine was also detected in the CSF via serum. These results indicate that some components of colostrum can be transported into the CSF via the serum, suggesting the possibility of modification of immature brain functions by colostral suckling in neonatal pigs.
Harada
E
E
Department of Veterinary Physiology, Faculty of Agriculture, Tottori University, Tottori, Japan. harada@muses.tottori-u.ac.jp
Sugiyama
A
A
Takeuchi
T
T
Sitizyo
K
K
Syuto
B
B
Yajima
T
T
Kuwata
T
T
D000818:Animals; D000831:Animals, Newborn / Q000097:blood* / Q000134:cerebrospinal fluid*; D001692:Biological Transport / Q000502:physiology; D001798:Blood Proteins / Q000032:analysis; D002417:Cattle; D002556:Cerebrospinal Fluid Proteins / Q000097:blood / Q000378:metabolism; D003126:Colostrum / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism; D011247:Pregnancy; D013552:Swine / Q000097:blood* / Q000134:cerebrospinal fluid*
D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D007074:Immunoglobulin G; D007781:Lactoferrin