Colloidal gold filtrates as metal substrates for surface-enhanced infrared absorption spectroscopy.
A new method for obtaining surface-enhanced infrared absorption (SEIRA) spectra of antibodies and antibody/antigen complexes has been developed. Antibodies attached to colloidal gold particles and then collected by filtration onto porous polyethylene membranes show enhanced spectral bands at 1080 and 990 cm-1 regardless of the antibody specificity. Attachment of a model antigen, glucose oxidase, to its specific antibody/colloid complex prior to collection produces enhanced bands at 1540, 1395, and 1250 cm-1. Similarly, when the antigen Salmonella is attached to its specific antibody/colloid complex prior to collection, a new enhanced band is observed at 1015 cm-1. Similarities and differences of the SEIRA spectra obtained on gold colloid are compared to previous work on gold films.
Seelenbinder
J A
JA
Partnership for Sensors and Surface Technology, University of Rhode Island, Kingston 02881, USA.
Brown
C W
CW
Pivarnik
P
P
Rand
A G
AG
D000906:Antibodies / Q000032:analysis*; D000907:Antibodies, Bacterial / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis; D017979:Gold Colloid / Q000737:chemistry*; D012475:Salmonella / Q000276:immunology; D013055:Spectrophotometry, Infrared / Q000379:methods*
D000906:Antibodies; D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D017979:Gold Colloid