Eosinophil and IgE responses of IL-5 transgenic mice experimentally infected with Nippostrongylus brasiliensis.
Eosinophil and IgE responses of interleukin (IL)-5 transgenic and normal C3H/HeN mice were studied after experimental infection with Nippostrongylus brasiliensis (Nb). Intestinal worms were recovered at day 5 post-infection (PI), and numbers of total white blood cells (WBC) and eosinophils, and total serum IgE and anti-hapten (dinitrophenyl) (DNP) specific IgE titers, were measured at days 0, 14 and 21 PI. IL-5 mice appeared resistant to Nb infection showing a significantly lower worm recovery rate than normal mice (P < 0.05). Total WBC and eosinophil counts (/mm3) were significantly increased in Nb infected normal mice (P < 0.05), but unchanged (total WBC) or decreased (eosinophils) in IL-5 mice at day 21 PI. The total serum IgE level remarkably increased in normal mice, but only a little in IL-5 mice at days 14 and 21 PI. Priming with DNP brought about more remarkable increases of the total and anti-DNP specific IgE in normal mice than in IL-5 mice. The results show that IL-5 mice are resistant to Nb infection, and that eosinophil and IgE responses in these mice are not augmented by Nb infection.
Chai
J Y
JY
Department of Parasitology, Seoul National University College of Medicine. cjy@plaza.snu.ac.kr
Shin
E H
EH
Takatsu
K
K
Matsumoto
N
N
Kojima
S
S
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000097:blood*; D015848:Interleukin-5 / Q000235:genetics*; D007958:Leukocyte Count; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic / Q000276:immunology; D009559:Nippostrongylus / Q000276:immunology; D017206:Strongylida Infections / Q000276:immunology*
D000909:Antibodies, Helminth; D015848:Interleukin-5; D007073:Immunoglobulin E