title: [Detection of TT virus in healthy volunteer].
abstract: A novel DNA virus, TT virus(TTV), has been reported in Japanese patient with non A to G posttransfusion hepatitis. We sought to determine whether TTV infection occurs in healthy volunteer, and to compared with DNA extraction methods and polymerase chain reaction(PCR) primer for TTV in diagnostic system. Using a nested PCR assay, serum sample of healthy volunteer serve our laboratory in Japan were examined for the presence of TTV DNA. Twenty of 90(22%) healthy volunteer were detected to have TTV sequences in their serum. Also, we found that DNA extraction methods with a modified phenol-chloroform method. Our result suggested that detection of TTV DNA are high ratio of adults in Japan and were necessary to take care of selected using diagnostic systems.
authors.affiliation: Gene-Diagnostic Center, Otsuka Assay Laboratory, Otsuka Pharmaceutical Co., Ltd.
authors.forename: T
authors.initials: T
authors.lastname: Hadama
authors.forename: S
authors.initials: S
authors.lastname: Takami
authors.forename: M
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002353:Carrier State / Q000453:epidemiology* / Q000821:virology; D004266:DNA Virus Infections / Q000453:epidemiology* / Q000821:virology; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006525:Hepatitis, Viral, Human / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D015415:Biomarkers; D004279:DNA, Viral