Comparison of AluI-induced frequencies of dicentrics and translocations in human lymphocytes by chromosome painting.
It has been shown repeatedly that following irradiation of human lymphocytes in the G0 stage, more translocations are induced than dicentrics. To check the role of DNA double-strand breaks (DSB) alone for the induction of symmetrical and asymmetrical chromosome aberrations, the frequencies of induced exchange aberrations by the restriction enzyme AluI were analyzed. The enzyme was introduced into cells using the pellet pipetting technique. Frequencies of induced translocations and dicentrics were determined using a chromosome painting assay with chromosome-specific DNA libraries for chromosomes 1, 4 and X (representing 16.8% of the human genome). The number of translocations detected was approximately 3-fold higher than the number of dicentrics, indicating that the increased frequency of translocations compared with dicentrics found in irradiated human lymphocytes does not result from DNA lesions other than DSB but from differential processing of DSB.
Chatterjee
S
S
MGC, Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Centre, The Netherlands.
Martínez-López
W
W
Grigorova
M
M
Darroudi
F
F
Obe
G
G
Natarajan
A T
AT
D002503:Centromere / Q000187:drug effects; D002869:Chromosome Aberrations / Q000235:genetics*; D020223:Chromosome Painting / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D014178:Translocation, Genetic / Q000235:genetics*
C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific