title: Optical mapping and its potential for large-scale sequencing projects.
abstract: Physical mapping has been rediscovered as an important component of large-scale sequencing projects. Restriction maps provide landmark sequences at defined intervals, and high-resolution restriction maps can be assembled from ensembles of single molecules by optical means. Such optical maps can be constructed from both large-insert clones and genomic DNA, and are used as a scaffold for accurately aligning sequence contigs generated by shotgun sequencing.
authors.affiliation: Wyeth-Ayerst Research, CNS Disorders, Princeton, NJ 08543, USA. astonc01@mcrcr.med.nyu.edu
authors.forename: C
authors.initials: C
authors.lastname: Aston
authors.forename: B
authors.initials: B
authors.lastname: Mishra
authors.forename: D C
authors.initials: DC
authors.lastname: Schwartz
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D055095:Optics and Photonics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA