Establishment and characterization of a novel ALL-L3 cell line (BALM-18): induction of apoptosis by anti-IgM and inhibition of apoptosis by bone marrow stroma cells.
A human acute lymphoblastic leukemia (ALL) cell line, BALM-18, was established from the peripheral blood specimen of a patient with B cell ALL L3 type (ALL-L3) at diagnosis using bone marrow stroma cells (BST) as feeder cells. The primary leukemia cells did not grow without feeder cells. As with the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10+, CD20+, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL-L3 and Burkitt's lymphoma. It also revealed a significantly low level of bcl-2 protein. Strikingly, anti-human IgM antibody did induce apoptosis in induction experiments. However, it was reversed by the addition of anti-CD40 antibody or BST cells, whereas the culture supernatant of the stroma cells did not show any effect on the inhibition of apoptosis. BALM-18 may be useful for analyzing both the mechanisms of anti-IgM induced apoptosis and signaling during the inhibition of apoptosis by CD40 or BST cells.
Matsuo
Y
Y
Fujisaki Cell Center, Hayashibara Biochemical Labs. Inc., Okayama, Japan. yomatsuo@hayashibara.co.jp
Sugimoto
A
A
Harashima
A
A
Nishizaki
C
C
Ishimaru
F
F
Kondo
E
E
Katayama
Y
Y
Harada
M
M
Orita
K
K
D000328:Adult; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000502:physiology*; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D001854:Bone Marrow Cells; D002051:Burkitt Lymphoma / Q000473:pathology*; D019013:CD40 Antigens / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D016130:Immunophenotyping; D007621:Karyotyping; D008297:Male; D017154:Stromal Cells; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019013:CD40 Antigens; D007075:Immunoglobulin M