title: Expression of cathepsin B-like enzyme activity in cell lysate from cultured human normal keratinocytes.
abstract: In the present study, we have found that the cell lysate from cultured human normal keratinocytes from foreskin (HFKs) hydrolyzed alpha-N-benzoyl-DL-arginine beta-naphthylamide (BANA), and the BANA hydrolysis occurred most under conditions of 37 degrees C and pH 6.0. This activity was strongly inhibited by leupeptin, which is an inhibitor to cathepsin B. These results suggested that the cell lysate from cultured HFKs contained cathepsin B-like enzyme activity. This is the first report to demonstrate that cathepsin B-like enzyme activity was expressed in the cell lysate from human normal keratinocytes.
authors.affiliation: Department of Dermatology, Shimane Medical University.
authors.forename: T
authors.initials: T
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Takata
authors.forename: D
authors.initials: D
authors.lastname: Ueda
authors.forename: S
authors.initials: S
authors.lastname: Dekio
mesh_terms: D001587:Benzoylarginine-2-Naphthylamide / Q000378:metabolism; D002401:Cathepsin B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D015603:Keratinocytes / Q000201:enzymology*; D007976:Leupeptins / Q000494:pharmacology; D008297:Male
chemical_list: D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D001587:Benzoylarginine-2-Naphthylamide; D002401:Cathepsin B; C032854:leupeptin