title: The rice inoculant strain Alcaligenes faecalis A15 is a nitrogen-fixing Pseudomonas stutzeri.
abstract: The taxonomic position of the nitrogen-fixing rice isolate A15, previously classified as Alcaligenes faecalis, was reinvestigated. On the basis of its small subunit ribosomal RNA (16S rRNA) sequence this strain identifies as Pseudomonas stutzeri. Phenotyping and fatty acid profiling confirm this result. DNA:DNA hybridisations, using the optical renaturation rate method, between strain A15 and Pseudomonas stutzeri LMG 11199T revealed a mean DNA-binding of 77%. The identification was further corroborated by comparative sequence analysis of the oprF gene, which encodes the major outer membrane protein of rRNA homology group I pseudomonads. Furthermore we determined the nifH sequence of this strain and of two putative diazotrophic Pseudomonas spp. and made a comparative analysis with sequences of other diazotrophs. These Pseudomonas NifH sequences cluster with NifH sequences isolated from the rice rhizosphere by PCR and of proteobacteria from the beta and gamma subclasses.
authors.affiliation: F. A. Janssens Laboratorium voor Genetica, Katholieke Universiteit Leuven, Heverlee, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Vermeiren
authors.forename: A
authors.initials: A
authors.lastname: Willems
authors.forename: G
authors.initials: G
authors.lastname: Schoofs
authors.forename: R
authors.initials: R
authors.lastname: de Mot
authors.forename: V
authors.initials: V
authors.lastname: Keijers
authors.forename: W
authors.initials: W
authors.lastname: Hai
authors.forename: J
authors.initials: J
authors.lastname: Vanderleyden
mesh_terms: D000421:Alcaligenes / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames / Q000235:genetics; D012275:Oryza / Q000382:microbiology; D010641:Phenotype; D010802:Phylogeny; D011549:Pseudomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S