Inhibitory effect of resveratrol on interleukin 6 release by stimulated peritoneal macrophages of mice.
In the present investigation, interleukin 6 (IL-6) activity in the supernatant of cultured mouse peritoneal macrophages was monitored using a sensitive bioassay involving the IL-6-dependent murine hybridoma B9 cell line. The effects of resveratrol on Il-6 release by mouse peritoneal macrophages stimulated with calcium ionophore A23187 and fMLP were explored. Resveratrol, at a concentration range from 5 x 10(-6) to 4 x 10(-5) mol.l-1, was found to dose-dependently inhibit IL-6 release by cultured macrophages induced by A23187 and fMLP, and showed no direct cytotoxic effect, but induced proliferation of cultured mouse thymus cells. Resveratrol, at a concentration range from 10(-8) to 10(-5) mol.l-1, was shown to dose-dependently inhibit calcium ion influx into the cells with the stimulation of fMLP (10(-6) mol.l-1). These results suggest that the blocking of calcium ion influx into cells by reveratrol is one of the possible mechanisms of the IL-6 biosynthesis inhibitory action of resveratrol.
Zhong
M
M
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Cheng
G F
GF
Wang
W J
WJ
Guo
Y
Y
Zhu
X Y
XY
Zhang
J T
JT
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism; D017136:Ion Transport; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000093242:Interleukin-6 Inhibitors
D000001:Calcimycin; D002118:Calcium; D015850:Interleukin-6; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000077185:Resveratrol; D013267:Stilbenes; D000093242:Interleukin-6 Inhibitors