Retroviral-fibronectin interactions in transduction of mammalian cells.
Hematopoiesis occurs in a complex environment in the medullary cavity in close proximity to stromal cells, fibroblasts, endothelial cells and matrix molecules. Hematopoietic cell interactions in this environment appear to involve both integrin and proteoglycan-mediated cell-cell and cell-matrix interactions. Genetic transduction of hematopoietic stem cells via retroviral vectors has been hampered by low efficiency of gene transfer. Recently, hematopoietic stem cell adhesion to the extracellular matrix molecule fibronectin has been shown to increase transduction of these target cells using retrovirus vectors. The mechanism of increased transduction appears to involve colocalization of virus particles and target cells. These data are reviewed in this paper.
Williams
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Section of Pediatric Hematology/Oncology, Herman B Wells Center for Pediatric Research, Riley Hospital for Children, Howard Hughes Medical Institute, Indiana University School of Medicine, Indianapolis 46202, USA. dwilliam@iupui.edu
D000818:Animals; D002448:Cell Adhesion; D005353:Fibronectins / Q000235:genetics / Q000502:physiology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008322:Mammals; D012190:Retroviridae
D005353:Fibronectins