Simultaneous analysis of ketamine and bupivacaine in plasma by high-performance liquid chromatography.
A reversed-phase HPLC technique for the simultaneous measurement of both bupivacaine and ketamine in plasma is described. Plasma samples (0.5 ml) were prepared using a rapid and simple back-extraction technique. Resolution of both analytes and the internal standard, desipramine, from medicines coadministered to surgical paediatric patients was obtained using a 5 microm cyano (CN) (250x4.6 mm) column and a mobile phase comprising methanol-acetonitrile-orthophosphoric acid-0.01 M sodium dihydrogenphosphate (200:80:2:718). Good sensitivity for both analytes was observed using UV detection at a wavelength of 215 nm. The method has been validated according to the criteria established by the Journal of Chromatography B.
Gross
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Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire et Groupe NPPUA, Faculté de Médecine, Clermont-Ferrand, France.
Nicolay
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Eschalier
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D002045:Bupivacaine / Q000097:blood* / Q000493:pharmacokinetics; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007649:Ketamine / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007649:Ketamine; D002045:Bupivacaine