title: A thin glycoprotein coating of a synthetic lenticule does not cause nutritional deficiency of the anterior cornea.
abstract: OBJECTIVE
  This study investigated whether a glycoprotein coating that will be used to enhance corneal epithelialization affects in situ nutritional passage through a permeable membrane.
  
  
  METHODS
  Sixteen adult cats were equally divided into two groups. Polycarbonate membranes with pore size of 0.1 microm and total pore area (porosity) of 3.1% were used as implant materials. The membranes for Group 1 were coated with a thin layer of Collagen I, while the membranes for Group 2 were uncoated. Each membrane with 8-mm diameter was implanted into an interlamellar pocket of the cornea. The eyes were observed for approximately 35 days to monitor clinical signs of nutritional deficiency of the cornea, and then 7 membranes were removed from the eyes. The permeability of the explanted membranes to glucose, inulin and albumin was used to predict the in situ difference between the coated and uncoated groups in regard to nutritional passage through the membranes. To investigate the long-term effect of the surface coating on corneal health, two animals from Group 1 were followed for up to two years and then both eyes of each animal underwent histological examination.
  
  
  RESULTS
  Clinically, no post-surgical complications associated with nutritional deficiency were observed in any of the eyes. Nutritional permeability tests showed no significant differences between the coated and uncoated membranes. Histologically, the long-term animals showed no abnormal morphology associated with nutritional deficiency in the cornea anterior or posterior to the membranes.
  
  
  CONCLUSIONS
  A thin glycoprotein coating on a permeable membrane does not appear to affect the nutritional supply of the anterior cornea and therefore can be used to enhance epithelialization of synthetic corneal onlays in vivo.
authors.affiliation: Cooperative Research Centre for Eye Research and Technology, University of New South Wales, Sydney, Australia.
authors.forename: R Z
authors.initials: RZ
authors.lastname: Xie
authors.forename: D F
authors.initials: DF
authors.lastname: Sweeney
authors.forename: H J
authors.initials: HJ
authors.lastname: Griesser
authors.forename: S
authors.initials: S
authors.lastname: Tout
authors.forename: H Y
authors.initials: HY
authors.lastname: Cheng
authors.forename: J G
authors.initials: JG
authors.lastname: Steele
mesh_terms: D000818:Animals; D002415:Cats; D003315:Cornea / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D006023:Glycoproteins; D008567:Membranes, Artificial; D019736:Prostheses and Implants; D012016:Reference Values; D013499:Surface Properties
chemical_list: D006023:Glycoproteins; D008567:Membranes, Artificial