Prophylaxis of local vascular graft infection with levofloxacin incorporated into albumin-sealed dacron graft (LVFX-ALB Graft).
An animal model was used to assess the efficacy of levofloxacin (LVFX) incorporated into albumin (ALB)-sealed Dacron (LVFX-ALB) graft for the prevention of vascular graft infections caused by Staphylococcus aureus. Under general anesthetic, an interposition graft was placed into dog carotid artery. On completion of the operation, 0.1 ml of normal saline containing 10(7) colony-forming units (CFU) of a slime-producing S. aureus was inoculated directly onto the graft. After 1 day, the samples were sterilely harvested. The antibacterial activity of LVFX into the LVFX-ALB graft was evaluated by colony counting in bacterial cultures and by the fluorescent antibody method staining bacteria adhesion to the grafts. LVFX-ALB grafts had a lower infection rate than the control grafts (1/4, 10(2) CFU vs 4/4, 1.50 x 10(5)+/-1.38 x 10(5)CFU (mean+/-SE)). In an immunostaining study, LVFX-ALB grafts had small fluorescent areas showing S. aureus adhesion, while fluorescence was observed over the entire surface of the control grafts. Therefore, LVFX-ALB presumably had a bactericidal action and adhesive prevention against inoculated S. aureus. LVFX-ALB may be useful in preventing graft infections during and immediately after vascular reconstruction.
Osada
T
T
Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University School of Medicine, Aichi, Japan. t-osada@med.nagoya-u.ac.jp
Yamamura
K
K
Fujimoto
K
K
Mizuno
K
K
Sakurai
T
T
Ohta
M
M
Nabeshima
T
T
D000418:Albumins; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004285:Dogs; D006801:Humans; D064704:Levofloxacin; D015242:Ofloxacin / Q000494:pharmacology*; D011093:Polyethylene Terephthalates; D011817:Rabbits; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control*
D000418:Albumins; D000890:Anti-Infective Agents; D011093:Polyethylene Terephthalates; D064704:Levofloxacin; D015242:Ofloxacin