Histochemical and morphological identification of Kupffer cells activated by cisplatin.
Cisplatin is a potent anti-cancer agent which has been shown to activate Kupffer cells. These activated macrophages demonstrate an increase in extensions, lysosomes, and peroxisomes increasing their anti-tumor activity. Wistar rats were treated with cisplatin (9 mg/kg) and sections of liver were excised for light and electron microscopic analysis at 1, 6, 15, and thirty days post treatment. Non-specific esterase staining was used to differentiate Kupffer cells using light microscopy, morphologic criteria were used for TEM analysis. Liver sections taken 6 days post treatment showed the greatest number of activated macrophages, with the highest degree of activation. Interaction between natural killer cells and Kupffer cells was only seen 6 days post treatment. These results show that cisplatin's ability to enhance the immune system requires several days post treatment to reach maximum potency.
Telgenhoff
D J
DJ
Department of Zoology, Michigan State University, East Lansing 48824, USA.
Aggarwal
S K
SK
Johnson
B N
BN
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D007728:Kupffer Cells / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000970:Antineoplastic Agents; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D002945:Cisplatin