title: Mutations affecting either generation or survival of cells influence the pool size of mature B cells.
abstract: The mature B cell compartment of MHC class II-deficient B6 I-Aalpha(-/-) and the btk-defective CBA/N mouse strain is 4- to 5-fold smaller than in wild-type B6 mice. The defect in B6 I-Aalpha(-/-) mice is intrinsic to B cells and due to a 4- to 5-fold reduced lifespan, which however can be normalized by an I-Ealpha(d) transgene, but only when expressed early during B cell development. The reduced number of mature B cells in the btk-defective CBA/N mouse is due to a 4- to 5-fold lower number of immature splenic B cells entering the mature compartment. The combined defects of reduced lifespan and impaired generation in double mutant mice result in a severe deficiency in the mature B cell pool.
authors.affiliation: Basel Institute for Immunology, Switzerland. antibody@bii.ch
authors.forename: A G
authors.initials: AG
authors.lastname: Rolink
authors.forename: T
authors.initials: T
authors.lastname: Brocker
authors.forename: H
authors.initials: H
authors.lastname: Bluethmann
authors.forename: M H
authors.initials: MH
authors.lastname: Kosco-Vilbois
authors.forename: J
authors.initials: J
authors.lastname: Andersson
authors.forename: F
authors.initials: F
authors.lastname: Melchers
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival; D006207:Half-Life; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains / Q000502:physiology*; D013154:Spleen / Q000166:cytology
chemical_list: D000949:Histocompatibility Antigens Class II