title: Characterization of the Tra2 region of the IncHI1 plasmid R27.
abstract: In this study, the DNA sequence of one of the transfer regions of the IncHI1 plasmid R27 was determined. This region, which corresponds to coordinates 0-40 on the R27 map has been called the Tra2 region, and is believed to be involved in mating pair formation. DNA sequence analysis of the transfer region identified 11 open reading frames which showed similarities to the transfer genes from other conjugative systems. The R27 transfer genes appear to most closely resemble the genes from the F plasmid and Sphingomonas aromaticivorans plasmid pNL1, both within the individual genes and in the overall gene order. The Tra2 region is also distinct in that replication, partitioning, and stability genes are found in the middle of the transfer region. The R27 Tra2 region also contains a gene, trhF, which appears to be related to the TraF genes of Agrobacterium and Rhizobium species. This, along with the temperature-sensitive transfer system found in both H plasmids and Agrobacterium, leads to the speculation that the R27 transfer region evolved from both ancestral F-like and P-like plasmids.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
authors.forename: M M
authors.initials: MM
authors.lastname: Rooker
authors.forename: C
authors.initials: C
authors.lastname: Sherburne
authors.forename: T D
authors.initials: TD
authors.lastname: Lawley
authors.forename: D E
authors.initials: DE
authors.lastname: Taylor
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D017355:Zymomonas / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers