Leukocyte glycolysis and lactate output in animal sepsis and ex vivo human blood.
Lactate is released in large quantity from sites of sepsis and inflammation. We asked whether the increased lactate production found in sepsis can be explained by the augmented glycolysis of inflammatory cells. The glycolytic metabolism of rat peritoneal leukocytes was measured following cecal ligation and perforation (CLP) or sham laparotomy. CLP augmented glucose uptake, the pentose phosphate pathway, and glucose oxidation. Lactate output increased from 1.03 +/- 0.05 to 1.20 +/- 0.05 fmol x cell(-1) x min(-1) (P < .001). Total lactate output of peritoneal lavage fluid increased from 7.94 +/- 2.59 to 28.12 +/- 5.60 nmol L x min(-1) (P < .005). The effect of lipopolysaccharide (LPS) on the lactate output of whole blood from 31 critically ill patients was measured. Leukocyte lactate production was calculated by multiple linear regression analysis. Following exposure to LPS, human leukocyte lactate output increased from 0.20 +/- 0.09 to 1.22 +/- 0.14 fmol x cell(-1) x min(-1) (P < .001). This rate of production is so high that it suggests that the lactate output of different tissue beds in sepsis may be affected by their different cell populations and state of activation. This study supports the hypothesis that lactate may be more a product of inflammation than a marker of tissue hypoxia in sepsis.
Haji-Michael
P G
PG
Department of Intensive Care, Erasmus Hospital, Brussels, Belgium.
Ladrière
L
L
Sener
A
A
Vincent
J L
JL
Malaisse
W J
WJ
D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism; D016638:Critical Illness; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006019:Glycolysis; D006801:Humans; D000860:Hypoxia / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood*; D007962:Leukocytes / Q000378:metabolism*; D016014:Linear Models; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000097:blood; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000378:metabolism*
D015415:Biomarkers; D008070:Lipopolysaccharides; D019344:Lactic Acid