Rapid detection of respiratory viruses by shell vial assay using simultaneous culture of HEp-2, LLC-MK2, and MDCK cells in a single vial.
A shell vial assay with simultaneous culture of HEp-2, LLC-MK2, and MDCK cell lines in a single tube (CoHLM SV assay) was compared with traditional tube culture (TC) for the detection of the main respiratory viruses in 358 nasal wash specimens. A total of 170 strains were isolated from 168 virus-positive samples. A total of 94. 1% of the strains (160 strains; 128 respiratory syncytial viruses and 32 other viruses) were detected by the CoHLM SV assay in 48 h, whereas 98.2% of the strains (167 strains; 132 respiratory syncytial viruses and 35 other viruses) were detected by TC in a mean time of 6 days. The CoHLM SV assay may be useful for the rapid detection of respiratory viruses.
Navarro-Marí
J M
JM
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain.
Sanbonmatsu-Gámez
S
S
Pérez-Ruiz
M
M
De La Rosa-Fraile
M
M
D000818:Animals; D002460:Cell Line; D004285:Dogs; D006801:Humans; D009297:Nasal Mucosa / Q000821:virology*; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010252:Paramyxoviridae / Q000302:isolation & purification*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology; D013048:Specimen Handling; D014407:Tumor Cells, Cultured; D014773:Virology / Q000379:methods