Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees.
An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.
Haruta
M
M
Department of Nutrition and Food Science, Ochanomizu University, Tokyo, Japan.
Murata
M
M
Kadokura
H
H
Homma
S
S
D000595:Amino Acid Sequence; D000906:Antibodies; D015139:Blotting, Southern; D004156:Catechol Oxidase / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D003429:Cross Reactions; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005638:Fruit; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014197:Trees / Q000201:enzymology*
D000906:Antibodies; D018744:DNA, Plant; D004156:Catechol Oxidase