Isolation and expression of a nitrogen regulatory gene, nmc, of Penicillium roqueforti.
The nmc gene, encoding a global nitrogen regulator, has been cloned and characterized from Penicillium roqueforti, a fungus used in the dairy industry. The deduced amino acid sequence predicts a protein of 860 amino acids in length whose zinc finger DNA binding domain is at least 94% identical to those of the homologous fungal proteins. Northern blot analysis showed that nmc expression is induced by nitrogen starvation and not repressed by variation of the external pH.
Gente
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Laboratoire de Biologie Cellulaire Fongique (bât. 405), Centre de Microbiologie et Génétique, UMR-CNRS 5577, Université Claude Bernard Lyon I, Villeurbanne, France. gente@biomserv.univ-lyon1.fr
Poussereau
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Fèvre
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D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D010407:Penicillium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
C120271:AREA protein, Penicillium roqueforti; D005656:Fungal Proteins; D009584:Nitrogen