Cloning and characterization of the gene encoding Pasteurella haemolytica FnrP, a regulator of the Escherichia coli silent hemolysin sheA.
A Pasteurella haemolytica A1 gene was identified from a recombinant library clone that expressed hemolysis in host Escherichia coli cells. The gene, designated fnrP, had sequence identity to E. coli fnr, a global transcriptional regulator of genes required for conversion to anaerobic growth. FnrP complemented anaerobic deficiencies of a fnr-null mutant strain of E. coli and increased expression of the Fnr-dependent, anaerobic terminal reductase gene, frdA. FnrP was purified, identified by immunoblotting, and shown to be nonhemolytic. When FnrP was expressed in E. coli DeltasheA, a null mutant of the cryptic hemolysin SheA, the transformants were nonhemolytic, indicating that FnrP activates this silent hemolysin.
Uhlich
G A
GA
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506, USA.
McNamara
P J
PJ
Iandolo
J J
JJ
Mosier
D A
DA
D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D006460:Hemolysin Proteins / Q000235:genetics*; D006461:Hemolysis; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012756:Sheep; D013385:Succinate Dehydrogenase / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D006460:Hemolysin Proteins; D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C119944:hlyE protein, E coli; D013385:Succinate Dehydrogenase