Scavenging effect of benzophenones on the oxidative stress of skeletal muscle cells.
Benzophenone is an ultraviolet (UV)-absorbing agent that has been used in industry and medicine for more than 30 years. Consumers of cosmetics and sunscreens containing UV-absorbers are exposed to benzophenones on a daily basis, owing to the widespread use of these compounds. However, the efficacy of these compounds as scavengers of oxidative stress is still not well established. In the present study, we investigate the antioxidative capacity of six sunscreen benzophenone compounds. A primary myoblast culture was mixed in vitro with 100 microM menadione. The cytotoxic effect by menadione-induced oxidative stress was monitored by the lucigenin- or luminol-amplified chemiluminescence, methylthiotetrazole (MTT) assay, and the antioxidative effects of various benzophenone compounds were evaluated. The results showed that the addition of menadione can induce oxidative stress on myoblasts by superoxide and hydrogen peroxide production, which can be eradicated by superoxide dismutase (SOD) and catalase, respectively, in a dose-dependent mode. The catalase has a protective effect on the cytotoxicity induced by menadione as measured by the MTT assay, while the SOD does not. The selected benzophenones also have a significant scavenging effect on the menadione-induced cell death on the myoblasts. The ortho-dihydroxyl structure and other hydroxy groups in the same ring have a stronger scavenging effect on the superoxide anion on myoblasts; thus, a stable penoxy radical may be formed. The mechanism of this effect remains to be clarified.
Sun
J S
JS
Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, ROC.
Shieh
K M
KM
Chiang
H C
HC
Sheu
S Y
SY
Hang
Y S
YS
Lu
F J
FJ
Tsuang
Y H
YH
D000166:Acridines; D000818:Animals; D001577:Benzophenones / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013473:Sunscreening Agents / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014812:Vitamin K / Q000633:toxicity
D000166:Acridines; D001577:Benzophenones; D016166:Free Radical Scavengers; D013473:Sunscreening Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014812:Vitamin K; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D013482:Superoxide Dismutase; C022616:thiazolyl blue