title: Reevaluation of phylogeny in the Drosophila obscura species group based on combined analysis of nucleotide sequences.
abstract: The Drosophila obscura species group has served as an important model system in many evolutionary and population genetic studies. Despite the amount of study this group has received, some phylogenetic relationships remain unclear. While individual analysis of different nuclear, mitochondrial, allozyme, restriction fragment, and morphological data partitions are able to discern relationships among closely related species, they are unable to resolve relationships among the five obscura species subgroups. A combined analysis of several nucleotide data sets is able to provide resolution and support for some nodes not seen or well supported in analyses of individual loci. A phylogeny of the obscura species group based on combined analysis of nucleotide sequences from six mitochondrial and five nuclear loci is presented here. The results of several different combined analyses indicate that the Old World obscura and subobscura subgroups form a monophyletic clade, although they are unable to resolve the relationships among the major lineages within the obscura species group.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Arizona, Tucson 85721, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: O'Grady
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D002965:Classification; D004247:DNA / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004330:Drosophila / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D017344:Genes, Insect; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D009711:Nucleotides; D004247:DNA