Effects of ciclosporin on whole blood chemiluminescence of renal transplant patients.
OBJECTIVE
To examine the possible inhibitory role of ciclosporin (Cic) on luminol-dependent chemiluminescence (CL) of whole blood in renal transplant patients.


METHODS
Luminol-dependent CL was used to measure active oxygen species generation in respiratory burst of whole blood stimulated by zymosan A. Fluorescence polarization immunoassay was used to monitor the blood concentration of Cic.


RESULTS
CL values of Cic group (n = 50) decreased in comparison with those of normal group (n = 10) (P < 0.01). The blood concentration of Cic was negatively related to CL value (P < 0.01). The serum of renal transplant patients directly inhibited respiratory burst of peritoneal macrophages of rats in a concentration-dependent manner.


CONCLUSIONS
Cic inhibits the phagocytic activity of neutrophils in renal transplant patients.
Chen
S Y
SY
Department of Clinical Pharmacology, Nanfang Hospital, First Military Medical University, Guangzhou, China. chensy@fimmu.edu.cn
Liu
S T
ST
Hou
L B
LB
Chen
Z L
ZL
D000328:Adult; D000818:Animals; D016572:Cyclosporine / Q000097:blood / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood / Q000494:pharmacology*; D016030:Kidney Transplantation; D008163:Luminescent Measurements; D017737:Macrophages, Peritoneal / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D016897:Respiratory Burst / Q000187:drug effects*
D007166:Immunosuppressive Agents; D016572:Cyclosporine