Identification of cDNAs for Sox-4, an HMG-Box protein, and a novel human homolog of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/delta12-PGJ2 in Hep3B cells.
We have examined specific genes whose expression is altered during apoptosis induced by prostaglandin (PG)A2 and Delta12-PGJ2 in human hepatocellular carcinoma Hep3B cells. Using mRNA differential display, we have identified two genes: one is specifically up-regulated and encodes for human Sox-4 (Sry-HMG box gene) and the other is significantly down-regulated and is the human homolog of yeast Ssf-1, a novel splicing factor. Northern blot analysis confirmed their differential expressions. Interestingly, Sox-4 was highly expressed in subcutaneous tumors grown in nude mice as a xenograft from Hep3B cells. These results suggest that the expression of Sox-4 may be related to the apoptosis pathway leading to cell death as well as to tumorigenesis, and that Ssf-1 gene may serve as a negative regulator of PGA2/Delta12-PGJ2-mediated Hep3B cell apoptosis.
Ahn
S G
SG
Department of Biochemistry, Research Institute of Molecular Genetics, College of Medicine, Catholic University of Korea, 505 Banpo-Dong, Socho-Gu, Seoul, 137-701, Korea.
Cho
G H
GH
Jeong
S Y
SY
Rhim
H
H
Choi
J Y
JY
Kim
I K
IK
D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D015536:Down-Regulation; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D011454:Prostaglandins A / Q000494:pharmacology*; D055751:SOXC Transcription Factors; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D018076:DNA, Complementary; D006609:High Mobility Group Proteins; D009687:Nuclear Proteins; C491917:PPAN protein, human; D011454:Prostaglandins A; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors; C114037:SSF1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C489990:Sox4 protein, mouse; D015534:Trans-Activators; C100008:prostaglandin A2