title: Revolution through genomics in investigative and discovery toxicology.
abstract: The remarkable technologic and methodologic advances spurred on by the Human Genome Project are being applied throughout the life sciences. In the field of toxicology, high-resolution assays now make it possible to discover virtually all the differences in gene expression brought on by exposure to a particular xenobiotic. There are 2 principal approaches used to build a catalog of changes in gene expression: hybridization microarrays and gel-based methods, such as differential display and AFLP-based mRNA finger-printing. The power of such approaches is exemplified by the identification of more than 300 genes that differ in expression level by at least 2-fold in response to the nongenotoxic rodent liver carcinogen phenobarbital.
authors.affiliation: Genomic Sequencing Center, Monsanto Life Sciences Company, St. Louis, Missouri 63198, USA. charles.p.rodi@monsanto.com
authors.forename: C P
authors.initials: CP
authors.lastname: Rodi
authors.forename: R T
authors.initials: RT
authors.lastname: Bunch
authors.forename: S W
authors.initials: SW
authors.lastname: Curtiss
authors.forename: L D
authors.initials: LD
authors.lastname: Kier
authors.forename: M A
authors.initials: MA
authors.lastname: Cabonce
authors.forename: J C
authors.initials: JC
authors.lastname: Davila
authors.forename: M D
authors.initials: MD
authors.lastname: Mitchell
authors.forename: C L
authors.initials: CL
authors.lastname: Alden
authors.forename: D L
authors.initials: DL
authors.lastname: Morris
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D005821:Genetic Techniques; D015894:Genome, Human; D006801:Humans; D014116:Toxicology