Measurement of urinary amino acids using high-performance liquid chromatography equipped with a strong cation exchange resin pre-column.
We determined the urinary amino acid concentrations by high-performance liquid chromatography (HPLC) using an eluent buffer which had been processed through a column packed with strong cation-exchange resin. With this column, ghost peaks resolved or much reduced in size and shifted to a post-arginine position. A clearer separation of peaks of urinary amino acids and ammonia was obtained. This could be due to the elimination of O-phthalaldehyde reactive substances in the eluent by the resin column. This method does not require a separate step to remove ammonia, unlike most of the methods currently used, and thus saves time.
Hara
K
K
Department of Clinical Laboratory, Kansai Medical University, Osaka, Japan.
Hijikata
Y
Y
Hiraoka
E
E
Ogawa
N
N
Takahashi
H
H
D000596:Amino Acids / Q000652:urine*; D001216:Asparagine / Q000652:urine; D001224:Aspartic Acid / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D018698:Glutamic Acid / Q000652:urine; D005973:Glutamine / Q000652:urine; D006801:Humans; D015203:Reproducibility of Results
D000596:Amino Acids; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D001216:Asparagine