title: [The role of phase contrast microscopy in the evaluation of cervix neck and vaginal biocenosis].
abstract: The aim of our study was to evaluate and compare the possibility of germ identification in cervico-vaginal infections of different origin by Ph cytology and classic fixated and stained (H-E) smears. We identified Lactobacillus vag., Gardnerella vag., short bacilli (Escherichia coli, Proteus, Klebsiella), mixed Coccus flora (diplococci, small and large cocci), Trichomonas vag., and Candidia alb. in cervico-vaginal smears. In endocervical, mature metaplastic or parabasal cells the elementary and cocoid bodies of Chlamydia trachomatis were possible to observed. The cytopathic effects caused by viral infections (HPV, HSV, CMV) were detected. The classic (koilocytosis, dyskeratosis) and discrete (binucleation, cracked cells, ghost cells, tadpole cells and large cytoplasmatic hyaline granulations) morphological changes must be confirmed by colposcopy and Digene Hybryde Capture analysis. The results of identification of vaginal biocenosis in Ph microscope and H-E smears were similar. The main benefit of Ph cytology is accuracy, speed and reliability of comprehensive information.
authors.affiliation: Kliniki Ginekologii i Onkologii Collegium Medicum Uniwersytu Jagiellonskiego w Krakowie.
authors.forename: A
authors.initials: A
authors.lastname: Basta
authors.forename: G
authors.initials: G
authors.lastname: Glab
authors.forename: M
authors.initials: M
authors.lastname: Strama
authors.forename: D
authors.initials: D
authors.lastname: Przeszlakowski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000473:pathology*; D005260:Female; D006801:Humans; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D002575:Uterine Cervicitis / Q000382:microbiology* / Q000473:pathology*; D014627:Vaginitis / Q000382:microbiology* / Q000473:pathology*; D014777:Virus Diseases / Q000473:pathology*
vernacular_title: Rola mikroskopii kontrastowo-fazowej w ocenie biocenozy szyjki macicy i pochwy.