title: In plants a putative isovaleryl-CoA-dehydrogenase is located in mitochondria.
abstract: In plants the degradation pathways of branched-chain amino acids have remained somewhat unclear with respect to both their biochemistry and their intracellular location. While biochemical evidence has localized some of the catabolic enzymes in peroxisomes/glyoxysomes, others cofractionate with mitochondria. We have now identified a candidate protein and corresponding cDNA for an enzyme of the leucine catabolic pathway, the isovaleryl-CoA-dehydrogenase (IVD). This polypeptide is a member of the acyl-CoA-dehydrogenase (ACDH) family and is encoded in the nuclear genome of Arabidopsis thaliana. Expression of the putative IVD gene in pea seedlings is documented by western blot analyses with an antibody against the mammalian IVD. Subcellular fractionation identifies the putative IVD enzyme in the mitochondrion. This localization suggests that in plants mitochondria contain at least part of the branched-chain amino acid degradation pathway(s).
authors.affiliation: Allgemeine Botanik, Universität Ulm, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Däschner
authors.forename: C
authors.initials: C
authors.lastname: Thalheim
authors.forename: C
authors.initials: C
authors.lastname: Guha
authors.forename: A
authors.initials: A
authors.lastname: Brennicke
authors.forename: S
authors.initials: S
authors.lastname: Binder
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D015153:Blotting, Western; D002458:Cell Fractionation; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D017343:Genes, Plant / Q000235:genetics; D050769:Isovaleryl-CoA Dehydrogenase; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D044925:Oxidoreductases Acting on CH-CH Group Donors; D018532:Pisum sativum / Q000201:enzymology; D012639:Seeds / Q000201:enzymology; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D050769:Isovaleryl-CoA Dehydrogenase