[Detection of a new tissue-specific enhancer in the ct6 region of the regulatory region of the drosophila cut locus].
The cut locus of Drosophila is an interesting example of a complex eukaryotic locus responsible for the development of many tissues and organs. Most of this locus is regulatory. The entire locus was cloned by Tchurikov et al. in 1986 and Blochlinger et al. in 1988. The wing ctn enhancer located 80 kb upstream of the promoter was earlier found in a 2.7 kb EcoRI-BamHI DNA fragment. The locus region 65-80 kb remote from the promoter was assumed to control the development of wings and vibrissae. We have found a new enhancer region in the ct6 region of the locus, which was in a 5 kb BamHI-EcoRI DNA fragment adjacent to the ctn enhancer. This region is responsible for the expression of the reporter lacZ gene in many tissues and organs at all stages of Drosophila development (at least in the intestine, Malpighian tubules, thoracic and abdominal sensory organs, thoracic ganglia and in ring glands). Thus, the region located 75 kb upstream of the promoter has some properties of the locus control region (LCR).
Pavlova
G V
GV
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Churikov
H A
HA
Korochkin
L I
LI
Ponomarenko
N A
NA
Shostak
N G
NG
D000818:Animals; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D018398:Homeodomain Proteins; D007763:Lac Operon; D019901:Locus Control Region; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D014157:Transcription Factors
D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C066381:ct protein, Drosophila
Obnaruzhenie novykh tkanespetsificheskikh énkhanserov v raĭone ct6 reguliatornoĭ oblasti lokusa cut drozofily.