Optical mapping and its potential for large-scale sequencing projects.
Physical mapping has been rediscovered as an important component of large-scale sequencing projects. Restriction maps provide landmark sequences at defined intervals, and high-resolution restriction maps can be assembled from ensembles of single molecules by optical means. Such optical maps can be constructed from both large-insert clones and genomic DNA, and are used as a scaffold for accurately aligning sequence contigs generated by shotgun sequencing.
Aston
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Wyeth-Ayerst Research, CNS Disorders, Princeton, NJ 08543, USA. astonc01@mcrcr.med.nyu.edu
Mishra
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Schwartz
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D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D055095:Optics and Photonics; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA