title: [Neuronal apoptosis in human prion diseases].
abstract: Neuronal loss is a salient feature of prion diseases; however, its causes and mechanisms are unclear. The possibility that it could occur through an apoptotic process has been postulated and this is consistent with the lack of inflammation in prion disorders as supported by experimental studies. In order to test this hypothesis in humans, we examined samples of frontal and temporal cerebral cortex, striatum, thalamus and cerebellum from 26 patients who died from prion diseases. They included 16 cases of Creutzfeldt-Jakob disease (5 sporadic cases, 5 familial, 3 iatrogenic, and 3 cases with the new variant), and 10 cases of fatal familial insomnia including 8 homozygotes methionine/methionone at codon 129 of the prion protein gene and 2 heterozygotes. These were compared with age and sex matched controls. Using in situ end labelling, we identified apoptotic neurons in all the cases of Creutzfeldt-Jakob disease. A single labelled neuron was found in the eldest control. Apoptotic neurons were mostly found in damaged regions and their presence and abundance seemed to correlate closely with neuronal loss. This supports the view that apoptosis of neurons is a feature of prion diseases and may contribute to the neuronal loss which is one of the main characteristics of these conditions. Neuronal apoptosis also correlated well with microglial activation as demonstrated by the expression of major histocompatibility complex class II antigens and axonal damage as identified by beta-amyloid protein precursor immunostaining. In contrast, we found no obvious relationship between the topography and severity of neuronal apoptosis and the type, topography and abundance of prion protein deposits as demonstrated by immunohistochemistry.
authors.affiliation: Laboratoire d'Anatomie Pathologique (Neuropathologie), Hôpital Raymond Poincaré-Faculté de Médecine Paris-Ouest, Garches, France.
authors.forename: F
authors.initials: F
authors.lastname: Gray
authors.forename: H
authors.initials: H
authors.lastname: Adle-Biassette
authors.forename: F
authors.initials: F
authors.lastname: Chrétien
authors.forename: T
authors.initials: T
authors.lastname: Ereau
authors.forename: M B
authors.initials: MB
authors.lastname: Delisle
authors.forename: C
authors.initials: C
authors.lastname: Vital
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000473:pathology*; D017096:Prion Diseases / Q000473:pathology*
vernacular_title: Apoptose neuronale au cours des maladies à prions.