title: Expression of lectinlike oxidized low-density lipoprotein receptor-1 in human atherosclerotic lesions.
abstract: BACKGROUND
  Oxidized LDL (Ox-LDL) seems to play key roles in atherogenesis. Lectinlike Ox-LDL receptor-1 (LOX-1) is a recently identified cell-surface receptor for Ox-LDL. The relationship of this novel receptor for Ox-LDL to atherogenesis, however, has not yet been clarified. In this study, we explored the expression of LOX-1 in the atherosclerotic lesions of human carotid arteries.
  
  
  RESULTS
  Using carotid endarterectomy specimens obtained from 21 patients and 2 samples of normal human aortas, we examined LOX-1 expression by reverse transcription-polymerase chain reaction and immunohistochemistry. In aortas without atherosclerosis, LOX-1 expression was undetectable by immunohistochemistry and negligible by reverse transcription-polymerase chain reaction. In carotid arteries, luminal endothelial cells covering early atherosclerotic lesions were more frequently positive for LOX-1 expression than those in advanced atherosclerotic lesions. Endothelial cells in the intimal neovasculature of advanced lesions also expressed LOX-1. In addition, macrophages and smooth muscle cells in the intima of advanced atherosclerotic plaques were positive for LOX-1 expression.
  
  
  CONCLUSIONS
  LOX-1 may play important roles in Ox-LDL uptake and subsequent functional alteration in the luminal endothelium in early atherosclerotic lesions and in intimal neovascular endothelial cells in advanced plaques. Furthermore, LOX-1 may also be involved in Ox-LDL uptake and subsequent foam cell transformation in macrophages and smooth muscle cells in the atherosclerotic intima.
authors.affiliation: Departments of Geriatric Medicine, and Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: N
authors.initials: N
authors.lastname: Kume
authors.forename: S
authors.initials: S
authors.lastname: Miyamoto
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: H
authors.initials: H
authors.lastname: Moriwaki
authors.forename: T
authors.initials: T
authors.lastname: Murase
authors.forename: T
authors.initials: T
authors.lastname: Sawamura
authors.forename: T
authors.initials: T
authors.lastname: Masaki
authors.forename: N
authors.initials: N
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Kita
mesh_terms: D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis / Q000276:immunology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology*; D016466:CHO Cells; D002339:Carotid Arteries / Q000737:chemistry / Q000473:pathology; D006224:Cricetinae; D004730:Endothelium, Vascular / Q000737:chemistry / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D037102:Lectins; D008264:Macrophages / Q000737:chemistry / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011973:Receptors, LDL / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E; D014162:Transfection; D017539:Tunica Intima / Q000737:chemistry / Q000473:pathology; D000097382:CD68 Molecule
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D037102:Lectins; D012333:RNA, Messenger; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C495137:OLR1 protein, human