[Application of competitive immunoenzyme assay for analysis of group B fumonisins].
A varriant of competitive ELISA, making it possible to detect fumonisin B1 (up to 0.4 ng/well) was developed, based on the use of specific polyclonal antibodies obtained by immunization of rabbits with a horseradish peroxidase conjugate of fumonisin B1. The minimum concentration of fumonisin B in water-acetonitrile extracts of corn grain, dry corn products, and feeds, detected by the ELISA version developed is 0.2 mg/kg.
Kononenko
G P
GP
Russian Research Institute of Veterinary Sanitary, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, Russia.
Burkin
A A
AA
Zotova
E B
EB
Soboleva
N A
NA
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002264:Carboxylic Acids / Q000032:analysis* / Q000276:immunology; D002274:Carcinogens, Environmental / Q000032:analysis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005516:Food Microbiology; D037341:Fumonisins; D009183:Mycotoxins / Q000032:analysis*; D010544:Peroxidases; D011817:Rabbits; D012710:Serum Albumin, Bovine; D003313:Zea mays
D000906:Antibodies; D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; D012710:Serum Albumin, Bovine; C056933:fumonisin B1; D010544:Peroxidases
Primenenie tverdofaznogo konkurentnogo immunofermentnogo analiza dlia opredeleniia fumonizinov gruppy B.