[The stimulating effect of destabilase, a component of Hirudo medicinalis salivary gland secretion, on sensory neuron neurite growth in organotypic culture].
The effect of destabilase, a component of Hirudo medicinalis salivary gland secret, was investigated in organotypic tissue culture of dorsal root ganglia (DRG) of 10-11-day old chick embryos. Native destabilase in concentrations 0.01 and 0.05 ng/ml was active, inducing a more intensive neurite growth in DRG that in the control. The stabilizing activity of destabilase was lost following reverse-phase chromatography. Neurite-stimulating effects of the drug "pyjavit" is due presumably to neurite-stimulating activity of destabilase.
Chalisova
N I
NI
Institute of Physiology, Russian Academy of Sciences, and Medical Institute, St. Petersburg.
Zhuravskiĭ
S G
SG
Penniiaĭnen
V A
VA
Berezhnoĭ
S N
SN
Artamonova
I I
II
Zavalova
L L
LL
Baskova
I P
IP
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D010450:Endopeptidases / Q000494:pharmacology*; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000187:drug effects*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D007865:Leeches; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D009475:Neurons, Afferent / Q000187:drug effects* / Q000648:ultrastructure; D012469:Salivary Glands / Q000378:metabolism*; D013268:Stimulation, Chemical
D009414:Nerve Growth Factors; D010450:Endopeptidases; C045348:fibrin destabilase
Stimuliruiushchee vliianie destabilazy, komponenta sekreta, sliunnykh zhelez meditsinskoĭ piiavki, na rost neĭritov chuvstvitel'nykh neĭronov v organotipicheskoĭ kul'ture.