BseSI, a restriction endonuclease from Bacillus stearothermophilus Jo 10-553, which recognizes the novel hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3'.
A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends.
Steponaviciene
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MBI Fermentas, Institute of Biotechnology, Graiciũno 8, 2028 Vilnius, Lithuania.
Maneliene
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Petrusyte
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Janulaitis
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D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D004269:DNA, Bacterial; D009841:Oligonucleotides; C120857:endodeoxyribonuclease BseSI; D015252:Deoxyribonucleases, Type II Site-Specific