Cloning and sequencing of the gilthead sea bream estrogen receptor cDNA.
We report here the complete nucleotide sequence of a cDNA clone containing the full-coding sequence of the Sparus aurata estrogen receptor (ER) isolated from an expression library prepared from gilthead sea bream liver poly A+ RNA. The library was screened using a single strand rainbow trout ER cDNA probe, corresponding to the C-D domain. The cDNA sequence containing an insert of 2369 nucleotides was found to encode a protein of 579 amino acids. The 5'- and 3'-untranslated regions of the message are 186 and 392 nucleotides long, respectively. The gilthead sea bream ER shows the higher homology with the ER of another perciform, Chrysophrys major (93%), moderate to high homology with Oreocromis aureus (78%) medaka (77%) and rainbow trout (70.7%) ERs and lower homology with japanese eel (45%), amphibian (47%), avian (48.5%) and mammalian (47-47.5%) ERs. The sequence homologies and phylogenetic analysis of the various ERs suggest that gilthead sea bream ER should be considered as a ER alpha-like.
Muñoz-Cueto
J A
JA
Departamento de Biologia Animal, Vegetal y Ecologia, Facultad de Ciencias del Mar, Universidad de Cádiz, Spain. munoz.cueto@uca.es
Burzawa-Gérard
E
E
Kah
O
O
Valotaire
Y
Y
Pakdel
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D011960:Receptors, Estrogen / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011960:Receptors, Estrogen