title: Reassessment of the role of CD8+ T cells in the induction of allograft tolerance by donor-specific blood transfusion.
abstract: Donor-specific allograft tolerance can be induced in adult rats by pregraft donor-specific blood transfusion (DST). We have previously shown that DST elicits in recipients the expansion of a donor-specific CD8+ T cell clone displaying the Vbeta18-Dbeta1-Jbeta2.7 TCR rearrangement, which rapidly infiltrates allografts after transplantation, suggesting a regulatory function for this clone in DST-induced tolerance. In this study, recipients were pretreated before grafting, using an anti-CD8 monoclonal antibody to deplete CD8+ T cells. CD8 depletion before DST and transplantation abrogated allograft tolerance, and the CD8+ T cell clone was absent from allografts. These effects were not observed when CD8 depletion was performed after DST but before transplantation. We conclude that CD8+ T cells play a role in the induction of allograft tolerance by DST.
authors.affiliation: INSERM Unité U 437 Immunointervention dans les allo- et xeno-transplantations and Institut de Transplantation et de Recherche en Transplantation, Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Douillard
authors.forename: C
authors.initials: C
authors.lastname: Vignes
authors.forename: R
authors.initials: R
authors.lastname: Josien
authors.forename: E
authors.initials: E
authors.lastname: Chiffoleau
authors.forename: J M
authors.initials: JM
authors.lastname: Heslan
authors.forename: V
authors.initials: V
authors.lastname: Proust
authors.forename: J P
authors.initials: JP
authors.lastname: Soulillou
authors.forename: M C
authors.initials: MC
authors.lastname: Cuturi
mesh_terms: D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000961:Antilymphocyte Serum / Q000008:administration & dosage; D001483:Base Sequence; D001803:Blood Transfusion; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015342:DNA Probes / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology; D007108:Immune Tolerance / Q000235:genetics; D008212:Lymphocyte Depletion; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D015342:DNA Probes