L-selectin expression enhances clonogenesis of CD34+ cord blood progenitors.
L-selectin, a surface adhesion glycoprotein expressed on leukocytes, has a well-established role in mediating inflammation and lymphocyte recirculation. Recent evidence suggests that L-selectin may also influence hematopoiesis. We observed that a greater proportion of CD34+ cells express L-selectin in cord blood compared with adult bone marrow, and we hypothesized that L-selectin expression is associated with enhanced clonogenic properties. To test this, we compared CD34+/L-selectin+ cells with CD34+/L-selectin- cells in hematopoietic clonogenic assays. From CD34+/L-selectin+ cell cultures, we observed a 3-fold increase of d 12-14 colony-forming unit-granulocyte/macrophage and multipotent progenitor cells, and a 5-fold enhancement of primitive d 21 high proliferative potential colony-forming cells compared with the progeny of CD34+/L-selectin- cells. We conclude that CD34+ cord blood cells expressing L-selectin are enriched in their clonogenic activity compared with cell fractions lacking L-selectin expression.
Koenig
J M
JM
Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
Baron
S
S
Luo
D
D
Benson
N A
NA
Deisseroth
A B
AB
D000328:Adult; D018952:Antigens, CD34 / Q000097:blood* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D003114:Colony-Forming Units Assay; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D006410:Hematopoiesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D019041:L-Selectin / Q000097:blood* / Q000378:metabolism; D011247:Pregnancy
D018952:Antigens, CD34; D019041:L-Selectin