Negative correlation between cyclophilin mRNA level in leukocytes of renal allograft recipients and ciclosporin concentration in whole blood.
OBJECTIVE
To study mRNA level of cyclophilin in the white blood cells (WBC) of the renal allograft recipients (RAR) and its correlation with ciclosporin concentration in whole blood.


METHODS
The cyclophilin mRNA levels and beta-actin as controls in the WBC of 47 RAR were measured by quantitative reverse transcription polymerase chain reaction. The blood ciclosporin assay utilized monoclonal antibody fluorescence polarization immunoassay.


RESULTS
With the increase of ciclosporin concentration in whole blood (from 62 micrograms.L-1 to 678 micrograms.L-1), relative cyclophilin mRNA level in the WBC of RAR decreased nonlinearly (from 1.1 to 0.03, r = 0.8195).


CONCLUSIONS
There was a negative correlation between the mRNA level of cyclophilin in the WBC of RAR and the ciclosporin concentration in whole blood.
Zhao
Q
Q
Molecular Biology Laboratory, Jinling Hospital, Nanjing, China.
Li
F Q
FQ
Wu
J G
JG
Chu
X M
XM
Zhou
Y
Y
Yao
X D
XD
D000293:Adolescent; D000328:Adult; D016572:Cyclosporine / Q000097:blood*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D016030:Kidney Transplantation; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D016572:Cyclosporine; D019696:Peptidylprolyl Isomerase