Influence of RecA on in vivo virulence and Shiga toxin 2 production in Escherichia coli pathogens.
The enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains 933 and 86-24 as well as the uropathogenic E. coli (UPEC) strain 536 were compared with their isogenic rec A mutants and rec A trans -complemented strains in intravenous lethality and lung toxicity assays in mice. While the wild-type EHEC strains were fully virulent, the virulence of the rec A mutants was strongly reduced. Complementation of the EHEC rec A mutants with the cloned E. coli recA gene restored their virulence capacity. The stx2EHEC mutant TUV86-2 as well as its isogenic rec A mutant were completely avirulent in both assays. In contrast, RecA had no influence on the virulence of UPEC strain 536. We conclude that the lethality observed with EHEC is presumably mainly due to Shiga toxin, which is severely down-regulated in the rec A mutants as a result of lacking spontaneous phage induction. Therefore, the EHEC rec A+strains 933 and 86-24 were compared for their Shiga toxin 2 (Stx2) production with the respective rec A-counterparts. The rec A mutants of the EHEC strains were significantly reduced in toxin synthesis and were devoid of Stx2 specific phage production. Complementation of the EHEC rec A mutants with the cloned rec A gene enabled the rec A mutants to restore toxin and phage production. These results suggest that the higher level of Stx2 synthesis in the EHEC strains is the result of a higher level of spontaneous Stx2 specific phage induction, which is controlled by RecA.
Fuchs
S
S
Lehrstuhl für Molekulare Infektionsbiologie, Universität Würzburg, Röntgenring 11, D-97070 Würzburg, Germany.
Mühldorfer
I
I
Donohue-Rolfe
A
A
Kerényi
M
M
Emödy
L
L
Alexiev
R
R
Nenkov
P
P
Hacker
J
J
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001435:Bacteriophages / Q000254:growth & development / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D005260:Female; D006460:Hemolysin Proteins / Q000032:analysis; D051379:Mice; D016685:Mitomycin / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation; D019384:Nucleic Acid Synthesis Inhibitors / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D022603:Shiga Toxins; D013047:Specific Pathogen-Free Organisms; D010948:Viral Plaque Assay; D014774:Virulence
D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D022603:Shiga Toxins; D016685:Mitomycin; D011938:Rec A Recombinases