Demonstration of a novel sulfotransferase in fetal bovine serum, which transfers sulfate to the C6 position of the GalNAc residue in the sequence iduronic acid alpha1-3GalNAc beta1-4iduronic acid in dermatan sulfate.
A novel sulfotransferase activity was discovered in fetal bovine serum using pig skin dermatan sulfate as an acceptor and [35S]3'-phosphoadenosine 5'-phosphosulfate as a sulfate donor. The enzyme was separated from chondroitin:GalNAc 6-O-sulfotransferase by chromatographic techniques. Enzymatic analysis of the reaction products demonstrated that the enzyme transferred sulfate to the C6 position of the GalNAc residue in the sequence -iduronic acid alpha1-3GalNAc beta1-4iduronic acid-. Thus, the enzyme has been identified as a hitherto unreported dermatan sulfate:GalNAc 6-O-sulfotransferase. The finding is in sharp contrast to the current concept that in dermatan sulfate biosynthesis GalNAc 4-O-sulfation is a prerequisite for iduronic acid formation by C5 epimerase.
Nadanaka
S
S
Department of Biochemistry, Kobe Pharmaceutical University, Japan.
Fujita
M
M
Sugahara
K
K
D000116:Acetylgalactosamine; D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D002413:Cations, Divalent / Q000494:pharmacology; D002417:Cattle; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000378:metabolism*; D005312:Fetal Blood; D005333:Fetus; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D012867:Skin; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000097:blood* / Q000302:isolation & purification; D013552:Swine; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism
D002413:Cations, Divalent; D014312:Trisaccharides; D003871:Dermatan Sulfate; D000244:Adenosine Diphosphate; C010840:adenosine 3'-phosphate-5'-phosphate; D015238:Sulfotransferases; D008274:Magnesium; D000116:Acetylgalactosamine