Effect of Coriaria lactone on cytosolic free calcium of cultured neurons from rat cerebral cortex.
OBJECTIVE
To study the effect of Coriaria lactone (CL) on cytosolic free calcium ([Ca2+]i) of cultured neurons from cerebral cortex.


METHODS
Primary neuron culture (14d) and AR-CM-MIC cation measurement system were used, the [Ca2+]i were measured. CL effect was observed by loading egtazic acid.


RESULTS
The [Ca2+]i of cultured neurons (99.4-103.4) nmol.L-1 was elevated concentration-dependently by CL (25-500) mumol.L-1 (P < 0.01). This effect disappeared after loading egtazic acid 5 mmol.L-1, but reappeared after adding CaCl2 to 1 mmol.L-1.


CONCLUSIONS
The [Ca2+]i of cultured neurons was elevated by CL, depending on extracellular Ca2+.
Zhu
X F
XF
Department of Brain Research, Tongji Medical University, Wuhan, China.
Zhao
X L
XL
Zhu
C G
CG
Wang
J X
JX
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005333:Fetus; D007783:Lactones / Q000302:isolation & purification / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism; D051381:Rats
D004365:Drugs, Chinese Herbal; D007783:Lactones; C049836:coriaria lactone; D002118:Calcium