title: Male sterility in transgenic mice expressing activin betaA subunit gene in testis.
abstract: Activins and inhibins, which are endocrine regulators of anterior pituitary function, have also been reported to participate in the paracrine and autocrine regulation of reproductive function. To determine the in vivo effects of overexpressed activin/inhibin, we generated transgenic mice carrying the human activin/inhibin betaA subunit mini gene under the regulatory control of the mouse methallothionein promoter. In one of the transgenic line analyzed, the betaA subunit gene was preferentially expressed in the testis. Ectopic and allochronic expression of the betaA gene started at 3 weeks after birth and transgenic male mice became sterile in the ensuing several weeks. Histological analysis revealed testicular degeneration in these mice. The results from this transgenic line strongly support the in vivo activity of activin/inhibin in male reproductive functions.
authors.affiliation: Institute of Applied Biochemistry, Institute of Basic Medical Sciences.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanimoto
authors.forename: K
authors.initials: K
authors.lastname: Tanimoto
authors.forename: F
authors.initials: F
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Horiguchi
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Yagami
authors.forename: A
authors.initials: A
authors.lastname: Fukamizu
mesh_terms: D000818:Animals; D006801:Humans; D007248:Infertility, Male; D028322:Inhibin-beta Subunits; D007265:Inhibins / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008957:Models, Genetic; D013737:Testis / Q000033:anatomy & histology / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: C103008:inhibin beta A subunit; D007265:Inhibins; D028322:Inhibin-beta Subunits