Distribution of the glycine transporter glyt-1 in mammalian and nonmammalian retinae.
We have examined the distribution of the glycine transporter glyt-1 in retinae of macaques, cats, rabbits, rats, and chickens. In all species, all glycine-containing amacrine cells expressed immunoreactivity for glyt-1, though the intensity of immunoreactivity for glyt-1 did not appear to directly correlate with the intensity of immunoreactivity for glycine in individual cells. A small subpopulation of glycine-immunoreactive displaced amacrine cells or ganglion cells also expressed glyt-1 in retinae from macaques, cats, chickens, and rats but not in retinae from rabbits. In addition, in all species examined, some displaced amacrine cells also contained glycine but did not express glyt-1. In monkeys, cats, and rats, populations of cells which we interpret as being glycine-containing interplexiform cells expressed glyt-1: these cells lacked a content of glutamate, suggesting they are not bipolar cells. The glycine-containing bipolar cells did not express glyt-1, suggesting that these cells probably acquired their content of glycine by other means such as via gap junctional connections with glycine-containing amacrine cells.
Pow
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Department of Physiology and Pharmacology, and Vision, Touch and Hearing Research Centre, University of Queensland, Brisbane, Australia.
Hendrickson
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D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002415:Cats; D002645:Chickens; D019084:Fluorescent Antibody Technique, Indirect; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D008253:Macaca mulatta; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; C493266:Slc6a9 protein, rat; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005998:Glycine