title: Interleukin-8 inhalation directly provokes bronchoconstriction in guinea pigs.
abstract: BACKGROUND
  Although it has been reported that the concentration of interleukin (IL)-8 in nasal lavage fluid and sputum and its production in bronchial epithelium were increased in asthmatic subjects, the direct effects of IL-8 on the airways in vivo is unclear.
  
  
  METHODS
  We examined bronchoconstriction in response to IL-8 inhalation through an endotracheal cannula in anesthetized, artificially ventilated guinea pigs.
  
  
  RESULTS
  Inhalation of IL-8 at concentrations of 1 and 10 microg/ml caused significant bronchoconstriction, as revealed by the elevation of pressure at the airway opening. Moreover, the bronchoconstriction induced by IL-8 was significantly inhibited by the antihistamines diphenhydramine and terfenadine, suggesting the involvement of histamine release in the IL-8-induced bronchoconstriction. No significant leukocyte infiltration was observed in the bronchoalveolar lavage fluid or histologic findings 25 min after the first IL-8 inhalation.
  
  
  CONCLUSIONS
  IL-8 provokes bronchoconstriction without leukocyte accumulation in the airways, mediated in part by histamine release, in guinea pigs.
authors.affiliation: Third Department of Internal Medicine, Kanazawa University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujimura
authors.forename: S
authors.initials: S
authors.lastname: Myou
authors.forename: M
authors.initials: M
authors.lastname: Nomura
authors.forename: M
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: A
authors.initials: A
authors.lastname: Harada
authors.forename: N
authors.initials: N
authors.lastname: Mukaida
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
authors.forename: A
authors.initials: A
authors.lastname: Nonomura
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016084:Bronchoconstriction / Q000187:drug effects* / Q000502:physiology; D004155:Diphenhydramine / Q000494:pharmacology; D006168:Guinea Pigs; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D016209:Interleukin-8 / Q000008:administration & dosage / Q000494:pharmacology*; D016593:Terfenadine / Q000494:pharmacology
chemical_list: D006634:Histamine H1 Antagonists; D016209:Interleukin-8; D016593:Terfenadine; D004155:Diphenhydramine