DNA delivery from polymer matrices for tissue engineering.
We have proposed engineering tissues by the incorporation and sustained release of plasmids encoding tissue-inductive proteins from polymer matrices. Matrices of poly(lactide-co-glycolide) (PLG) were loaded with plasmid, which was subsequently released over a period ranging from days to a month in vitro. Sustained delivery of plasmid DNA from matrices led to the transfection of large numbers of cells. Furthermore, in vivo delivery of a plasmid encoding platelet-derived growth factor enhanced matrix deposition and blood vessel formation in the developing tissue. This contrasts with direct injection of the plasmid, which did not significantly affect tissue formation. This method of DNA delivery may find utility in tissue engineering and gene therapy applications.
Shea
L D
LD
Department of Biologic and Materials Science, University of Michigan, Ann Arbor 48109-1078, USA.
Smiley
E
E
Bonadio
J
J
Mooney
D J
DJ
D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D018014:Gene Transfer Techniques; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D010957:Plasmids; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew
D001672:Biocompatible Materials; D010982:Platelet-Derived Growth Factor; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA