Cholinomimetic action of macrolide antibiotics on airway gland electrolyte secretion.
We investigated the acute effects of erythromycin (EM) and its derivatives on ionic currents in airway glands from feline tracheae. Therapeutic concentrations of EM or clarithromycin (CAM) attenuated the whole cell currents evoked by ACh in a competitive manner. The maximally stimulated inward Cl- currents were reduced to 54 and 83% and the outward K+ currents to 55 and 84% of control values by EM and CAM, respectively, whereas the responses induced by phenylephrine, norepinephrine, caffeine, or ionomycin were unaffected by EM, CAM, or EM523, a synthetic derivative of EM. K+ channels in excised outside-out patches were not influenced by macrolides. Although therapeutic concentrations of macrolides showed no effect on the baseline currents, high concentrations of macrolides alone evoked currents mimicking the ACh response, which were abolished completely by atropine. We concluded that macrolides act as a partial agonist on cholinergic receptors, resulting in a reduction of Cl- secretion at pharmacological doses of the agents, which may exhibit a pronounced effectiveness on hypertrophied and/or cholinergically sensitized submucosal glands in pathological airways.
Irokawa
T
T
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai 980-8574, Japan.
Sasaki
T
T
Shimura
S
S
Sasamori
K
K
Oshiro
T
T
Nara
M
M
Tamada
T
T
Shirato
K
K
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002415:Cats; D018678:Cholinergic Agents / Q000494:pharmacology*; D004553:Electric Conductivity; D004573:Electrolytes / Q000378:metabolism*; D004917:Erythromycin / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D009092:Mucous Membrane / Q000378:metabolism / Q000502:physiology; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D014132:Trachea / Q000378:metabolism* / Q000502:physiology
D000900:Anti-Bacterial Agents; D018678:Cholinergic Agents; D004573:Electrolytes; D015221:Potassium Channels; D004917:Erythromycin; D000109:Acetylcholine