Multi-enzymatic glucosylation using Eucalyptus UDP-glucosyltransferase coupled UDPglucose-fermentation by bakers' yeast.
The enzymatic synthesis of glucoside compounds using a membrane-associated UDP-glucosyltransferase fraction from Eucalyptus perriniana cultured cells as a water-insoluble catalyst (N. Nakajima, et. al., J. Ferment. Bioeng., 84 (5), pp. 455-460, 1997) has been effectively done by coupling UDPglucose-fermentation by bakers' yeast. For example, beta-thujaplicin (hinokitiol) and p-aminobenzoic acid were converted respectively to their corresponding beta-D-monoglucosides with the conversion rate of around 24-26% by the multi-enzymatic system with UDPglucose as a glucose donor, which is produced by yeast cells from glucose and 5'-UMP. Addition of either cellobiose, a substrate of beta-glucosidase, or DL-1,2-anhydro-myo-inositol, an inhibitor for the enzyme in the reaction mixture, could increased the yield of these beta-D-monoglucosides. This new enzymatic system could also be used for the synthesis of flavonoid glucosides such as isoquercitrin (quercetin 3-O-beta-D-glucoside).
Nakajima
N
N
Department of Nutritional Science, Okayama Prefectural University, Japan. nakajima@fhw.oka-pu.ac.jp
Ishihara
K
K
Hamada
H
H
Yamane
S
S
Nakamura
K
K
Furuya
T
T
D005052:Eucalyptus / Q000201:enzymology*; D005285:Fermentation; D005964:Glucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D010946:Plants, Medicinal; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*
D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose