Encephalitozoon cuniculi: light and electron microscopic evidence for di-, tetra-, and octosporous sporogony and a note on the molecular phylogeny of encephalitozoonidae.
We demonstrate, based on the light, electron microscopic, and immunofluorescence studies carried out on two isolates of Encephalitozoon cuniculi established in culture, that E. cuniculi exhibits di-, tri-, tetra- and octosporous sporogony. We therefore propose that the generic characters of Encephalitozoon should be amended to include tetra-sporous sporogony as generic features. Additionally, the molecular phylogenetic analysis indicates that E. cuniculi, E. hellem, and E. (Septata) intestinalis form a cohesive group.
Visvesvara
G S
GS
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. GSV1@CDC.GOV
Leitch
G J
GJ
Pieniazek
N J
NJ
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D016819:Encephalitozoon / Q000145:classification* / Q000235:genetics; D009670:Encephalitozoon cuniculi / Q000145:classification / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D013170:Spores / Q000502:physiology / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure
D016054:DNA, Protozoan; D012335:RNA, Ribosomal