title: [Serial change of cerebral blood flow after collagenase induced intracerebral hemorrhage in rats].
abstract: In case of intracerebral hemorrhage (ICH), many clinical studies have reported that great alterations in regional cerebral blood flow (rCBF) have been observed not only in the acute but also in the chronic stage. However, due to the absence of reproducible animal model, experimental studies have failed to confirm the evidence. We therefore studied the serial change of rCBF and investigated the possibility of secondary brain damage using our collagenase induced ICH model. A total of 60 adult male rats were examined. 2 microliters artificial cerebrospinal fluid, which was adjusted to a pH 7.4 by a buffer, containing 0.25 units of bacterial collagenase was stereotactically injected into the left caudoputamen in 28 rats. 2 microliters artificial cerebrospinal fluid without collagenase was also injected into 28 control rats under sham operation. The remaining 4 rats were studied as a non treatment group. The rCBF of the frontal cortex, parietal cortex, temporal cortex, occipital cortex, caudoputamen, thalamus and the cerebellum was determined bilaterally by [14C]-iodoantipyrine quantitative autoradiography according to Sakurada's method at 2, 4, 12, 24, 48, 168 and 720 hours after the operation. At 4 hours after injection, significant decrease of rCBF was observed in the ipsilateral hemisphere and also in some contralateral regions. In the ipsilateral frontal cortex and caudoputamen, there was significant increase of the rCBF which reached close to the baseline at 24 hours. After that, the rCBF of those regions decreased significantly again and remained at a low value even 1 month after injection. In the other regions of the ipsilateral hemisphere which were located remotedly from the hematoma, the rCBF recovered from 24 to 48 hours after injection. In the contralateral hemisphere, the rCBF also recovered at 24 hours. We suggest that this alteration of the rCBF observed in perihematomatous regions was the result of secondary brain damage caused by the existence of hematoma. On account of this, we conclude that early removal of hematoma is necessary to obtain a better result in cases of ICH.
authors.affiliation: Department of Neurosurgery, Nara Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yonezawa
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Sakaki
mesh_terms: D000818:Animals; D001345:Autoradiography; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D017364:Collagenases; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017364:Collagenases