Germline and somatic mutations of the STK11/LKB1 Peutz-Jeghers gene in pancreatic and biliary cancers.
Peutz-Jeghers syndrome (PJS) is an autosomal-dominant disorder characterized by hamartomatous polyps in the gastrointestinal tract and by pigmented macules of the lips, buccal mucosa, and digits. Less appreciated is the fact that PJS also predisposes patients to an increased risk of gastrointestinal cancer, and pancreatic cancer has been reported in many PJS patients. It was recently shown that germline mutations of the STK11/LKB1 gene are responsible for PJS. We investigated the role of STK11/LKB1 in the development of pancreatic and biliary cancer in patients with and without the PJS. In a PJS patient having a germline splice site mutation in the STK11/LKB1 gene, sequencing analysis of an intestinal polyp and pancreatic cancer from this patient revealed loss of the wild-type allele of the STK11/LKB1 gene in the cancer. Inactivation of STK11/LKB1, by homozygous deletions or somatic sequence mutations coupled with loss of heterozygosity, was also demonstrated in 4-6% of 127 sporadic pancreatic and biliary adenocarcinomas. Our results demonstrate that germline and somatic genetic alterations of the STK11/LKB1 gene may play a causal role in carcinogenesis and that the same gene contributes to the development of both sporadic and familial forms of cancer.
Su
G H
GH
Departments of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hruban
R H
RH
Bansal
R K
RK
Bova
G S
GS
Tang
D J
DJ
Shekher
M C
MC
Westerman
A M
AM
Entius
M M
MM
Goggins
M
M
Yeo
C J
CJ
Kern
S E
SE
D000091162:AMP-Activated Protein Kinase Kinases; D000230:Adenocarcinoma / Q000150:complications / Q000235:genetics*; D000328:Adult; D015139:Blotting, Southern; D002460:Cell Line; D003138:Common Bile Duct Neoplasms / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005819:Genetic Markers; D018095:Germ-Line Mutation; D006801:Humans; D019656:Loss of Heterozygosity; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000150:complications / Q000235:genetics*; D010580:Peutz-Jeghers Syndrome / Q000150:complications / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017384:Sequence Deletion
D005819:Genetic Markers; D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases