title: Tyrosine hydroxylase-immunoreactive elements in the human globus pallidus and subthalamic nucleus.
abstract: In contrast to the well-established dopaminergic innervation of the neostriatum, the existence of dopaminergic innervation of the subthalamic nucleus and globus pallidus is controversial. In the present study, tyrosine hydroxylase (TH)-immunoreactive elements were observed by light microscopy after antigen retrieval in the subthalamic nucleus and in the internal and external segments of the globus pallidus in postmortem human brain. Small islands of apparent neostriatal tissue with abundant arborization of fine, TH-immunoreactive axons in the vicinity of calbindin-positive small neurons resembling neostriatal medium spiny neurons were present in the external segment of the globus pallidus. Large numbers of medium-large, TH-immunoreactive axons were observed passing above and through the subthalamic nucleus and through both pallidal segments; these are presumed to be axons of passage on their way to the neostriatum. In addition, fine, TH-immunoreactive axons with meandering courses, occasional branches, and irregular outlines, morphologically suggestive of terminal axon arborizations with varicosities, were seen in both pallidal segments, including the ventral pallidum, and the subthalamic nucleus, consistent with a catecholaminergic (probably dopaminergic) innervation of these nuclei. This finding suggests that, in Parkinson's disease and in animal models of this disorder, loss of dopaminergic innervation might contribute to abnormal neuronal activation in these three nuclei.
authors.affiliation: Department of Psychiatry, New England Medical Center and the Tufts University School of Medicine, Boston, Massachusetts 02111, USA. jhedreen@opal.tufts.edu
authors.forename: J C
authors.initials: JC
authors.lastname: Hedreen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D001369:Axons / Q000201:enzymology; D004298:Dopamine / Q000378:metabolism; D005260:Female; D005917:Globus Pallidus / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D017072:Neostriatum / Q000166:cytology / Q000201:enzymology; D009474:Neurons / Q000201:enzymology*; D016612:Paraffin Embedding; D010300:Parkinson Disease / Q000378:metabolism; D013787:Thalamic Nuclei / Q000166:cytology* / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine