title: Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium.
abstract: An acid-stable soybean-peroxidase biosensor was developed by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 microA/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA).
authors.affiliation: Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, PRC.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Dong
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016877:Oxidants / Q000032:analysis*; D010544:Peroxidases / Q000737:chemistry*; D013025:Glycine max / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D016877:Oxidants; D006861:Hydrogen Peroxide; D010544:Peroxidases