title: Microglia in the human fetal spinal cord--patterns of distribution, morphology and phenotype.
abstract: Microglia, the intrinsic macrophages of the nervous system, colonise the cerebrum around the second trimester in man. In order to determine the extent of microglial influx into the nervous system, we have examined their distribution within the human fetal spinal cord in relation to astrocytic and vascular development between 9 and 16 weeks of gestation, using conventional immunohistochemistry [CD11b; CD45; CD64; CD68; ICAM-1; ICAM-2; VCAM-1; PECAM; GFAP; vimentin] and lectin histochemistry [RCA-1]. Microglia are identifiable by 9 weeks, within the ventricular/sub-ventricular zones. Human fetal microglia display heterogeneity in phenotype and are more readily identified by CD68 in the spinal cord. There is a marked influx of cells dorsal and ventral to the neural cavity, from the marginal layer [meninges/connective tissue] with advancing gestational age, with greatest cell densities towards the end of the time period in this study. This inward migration is associated with progressive vascularisation, ICAM-2 expression and co-localises with GFAP and vimentin positive radial glia. The patterns of microglial migration in human fetal cord differ from that within the cerebrum, but generally conform to a route following white to gray matter.
authors.affiliation: Department of Neuropathology, Institute of Psychiatry, De Crespigny Park, London SE5 8JN, UK. p.rezaie@iop.kcl.ac.uk
authors.forename: P
authors.initials: P
authors.lastname: Rezaie
authors.forename: K
authors.initials: K
authors.lastname: Patel
authors.forename: D K
authors.initials: DK
authors.lastname: Male
mesh_terms: D005333:Fetus / Q000648:ultrastructure*; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D017628:Microglia / Q000648:ultrastructure*; D010641:Phenotype; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology