Endothelial nitric oxide synthetase (eNOS) in astrocytes: another source of nitric oxide in neocortex.
The distribution of the endothelial form of nitric oxide synthetase (eNOS) was examined in the visual cortex of three species of primate and in the rat using immunocytochemistry. Labeled cells were found in both the gray and white matter. These cells were stellate in appearance and labeled cell processes were seen contacting blood vessels or the pia, suggesting that, by morphological criteria, the cells were astrocytes. All eNOS positive cells were double labeled with an antibody against S100beta. Although all cells were double labeled in the white matter, in the gray matter, some S100beta positive cells did not contain detectable levels of eNOS. eNOS positive astrocytic processes appeared to form prominent and distinctive structures next to neurons, especially in cortical layer IIIC. We postulate that these eNOS-positive structures form astrocytic perisynaptic sheaths on neuronal somas in the cortex. If this is true, then nitric oxide can influence neuronal transmission directly at axosomatic synapses in the cortex. In addition, the presence of eNOS in astrocytes and in their processes that contact blood vessels suggests that the link between local cortical activity and changes in cerebral blood flow could be mediated by astrocytic release of nitric oxide.
Wiencken
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AE
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232-2175, USA.
Casagrande
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VA
D000818:Animals; D001029:Aotus trivirgatus; D001253:Astrocytes / Q000201:enzymology*; D001921:Brain / Q000166:cytology / Q000201:enzymology; D002135:Calcium-Binding Proteins / Q000032:analysis; D005701:Galago; D009252:NADPH Dehydrogenase / Q000032:analysis; D019579:Neocortex / Q000201:enzymology*; D009414:Nerve Growth Factors / Q000032:analysis; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052250:Nitric Oxide Synthase Type III; D011323:Primates; D051381:Rats; D020318:Rats, Long-Evans; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012453:Saimiri
D002135:Calcium-Binding Proteins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D009252:NADPH Dehydrogenase