Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion.
The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-micros pulse length, and electric field of 0.2 kV cm(-1), can make erythrocyte-amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities.
Biswas
S
S
Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi.
Guha
S K
SK
D000650:Amnion / Q000166:cytology* / Q000648:ultrastructure; D002459:Cell Fusion; D004560:Electricity; D004912:Erythrocytes / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010920:Placenta / Q000166:cytology* / Q000648:ultrastructure