Electron microscopy of DNA-protein complexes and chromatin.
This article focused on a number of aspects of the preparation of chromatin and other DNA-protein complexes for conventional transmission EM that are critical for success but may not have been addressed in a single chapter before. These include the importance of optimizing fixation, the generation of active supporting supports, and the use of negative staining as a means of obtaining higher resolution detail than can be garnered from shadow casting methods.
Griffith
J
J
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill 27599, USA.
Michalowski
S
S
Makhov
A M
AM
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002645:Chickens; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism / Q000648:ultrastructure*; D004270:DNA, Circular / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure; D007202:Indicators and Reagents; D008854:Microscopy, Electron / Q000379:methods; D009707:Nucleosomes / Q000378:metabolism / Q000648:ultrastructure*; D013539:Simian virus 40 / Q000648:ultrastructure
D002843:Chromatin; D004270:DNA, Circular; D004268:DNA-Binding Proteins; D007202:Indicators and Reagents; D009707:Nucleosomes; D004247:DNA