title: Identification of a 24 kDa intrinsic membrane protein from mammalian peroxisomes.
abstract: A 24 kDa protein from rat liver peroxisomal membrane was isolated and subjected to Edman degradation. Using the N-terminal sequence of this polypeptide we have identified several rat and human expressed sequence tags in the GenBank Database. The complete sequence of a human cDNA clone was determined. The open reading frame encodes an extremely basic protein 212 amino acid residues long. A high similarity between this mammalian protein and hypothetical proteins from Caenorhabditis elegans and Neurospora crassa was found. Hydropathy analysis reveals the existence of two putative membrane-spanning domains in conserved regions of the three homologous proteins.
authors.affiliation: Unidade de Neurobiologia Genética do Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Reguenga
authors.forename: M E
authors.initials: ME
authors.lastname: Oliveira
authors.forename: A M
authors.initials: AM
authors.lastname: Gouveia
authors.forename: C
authors.initials: C
authors.lastname: Eckerskorn
authors.forename: C
authors.initials: C
authors.lastname: Sá-Miranda
authors.forename: J E
authors.initials: JE
authors.lastname: Azevedo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D006801:Humans; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008830:Microbodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment
chemical_list: D008565:Membrane Proteins; C120047:PXMP4 protein, human