Effects of in vitro cadmium exposure on ovarian steroidogenesis in rats.
The purpose of this study was to evaluate the direct effect(s) of in vitro cadmium (Cd) exposure on steroidogenesis in rat ovaries during different reproductive states. Sprague-Dawley rats were killed on the day of proestrus, or on gestation day 6 or 16. Ovaries were removed, placed in medium and minced. Culture from each ovary was incubated with Cd2+ ions in concentrations of 0, 100, 500, 1000, 1500, or 2000 microM. One-hour whole-ovary production of progesterone (P4), testosterone and estradiol (E2) in culture medium was evaluated in the absence and presence of human chorionic gonadotropin (hCG) or hCG plus pregnenolone by specific radioimmunoassay. Under in vitro Cd exposure the most affected were productions of P4 and testosterone in proestrus rats and less in pregnant dams, whereas E2 was not affected at all. Cadmium appears to interfere with the ovarian steroidogenic pathway in rats at more than one site.
Piasek
M
M
Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Zagreb, Republic of Croatia. mpiasek@imi.hr
Laskey
J W
JW
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005865:Gestational Age; D006801:Humans; D009924:Organ Culture Techniques; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011359:Proestrus / Q000187:drug effects / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis; D051381:Rats; D013256:Steroids / Q000096:biosynthesis*; D013739:Testosterone / Q000096:biosynthesis
D006063:Chorionic Gonadotropin; D013256:Steroids; D002104:Cadmium; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol