The human papillomavirus type 11 upstream regulatory region triggers hair-follicle-specific gene expression in transgenic mice.
We have generated transgenic mice carrying the URR of the human papillomavirus type 11 ligated in front of the Escherichia coli beta-galactosidase coding region sequence. Using X-Gal staining to demonstrate beta-galactosidase production, we observed a hair-specific transcription of the reporter gene. This transcription was limited to the epithelial cells of the hair bulge region. The transgene was developmentally regulated, as no LacZ staining was demonstrated during embryogenesis and specific staining was first observed after birth. Surprisingly, dexamethasone and ultraviolet B, but not phorbol myristate acetate or progesterone treatment of the animals resulted in an increase in number and intensity of hair follicles expressing the reporter gene.
Schenkel
J
J
Deutsces Krebsforschungszentrum, Heidelberg, Germany.
Gaissert
H
H
Protopapa
E E
EE
Weiher
H
H
Gissmann
L
L
Alonso
A
A
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D004847:Epithelial Cells / Q000821:virology; D005260:Female; D005629:Frozen Sections; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic; D018808:Transcription Factor AP-1 / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000187:drug effects / Q000235:genetics; D001616:beta-Galactosidase / Q000032:analysis
D018808:Transcription Factor AP-1; D003907:Dexamethasone; D001616:beta-Galactosidase