Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator.
In order to facilitate the isolation of pure cultures from natural habitats we have developed a method for the isolation of single microbial cell clones from a mixed population, e.g. the flora of the termite gut, with the aid of a modern micromanipulator. The separated single prokaryotic or eukaryotic cells were grown after transfer in culture media or they were used for single cell PCR. The micromanipulator was also applied for the removal of nuclei from protozoa, of which the SSU rDNA was directly amplified.
Fröhlich
J
J
Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität, Mainz, Germany. jfroehl@mail.uni-mainz.de
König
H
H
D000818:Animals; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D056890:Eukaryota / Q000302:isolation & purification; D008828:Microbiological Techniques / Q000295:instrumentation; D008846:Micromanipulation / Q000295:instrumentation; D010802:Phylogeny