Assay of humoral immunity to mumps virus.
One hundred randomly chosen sera from blood donors from North London were assayed for antibodies to mumps virus by plaque reduction and microtitre neutralisation assay, haemagglutination inhibition and in-house ELISA. The assay reproducibility was determined, and there was reasonable agreement between antibody levels measured by the two neutralising methods. Neutralising antibody levels measured by either method were low but the strain used in the assay had a large effect on the antibody titres observed. Titres measured by neutralisation assay, HI assay and ELISA did not correlate well. Assessment of immunity to mumps virus remains problematical.
Pipkin
P A
PA
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK.
Afzal
M A
MA
Heath
A B
AB
Minor
P D
PD
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008875:Middle Aged; D009109:Mumps virus / Q000276:immunology*; D009500:Neutralization Tests; D014709:Vero Cells; D010948:Viral Plaque Assay
D000914:Antibodies, Viral