Hypothermia causes a reversible, p53-mediated cell cycle arrest in cultured fibroblasts.
Normal human fibroblasts grown in cell culture undergo a reversible growth arrest when incubated at 28 degrees C. During incubation at 28 degrees C, levels of p53 and p21 rise in these cells and cell cycle analysis shows that they have undergone a cell cycle arrest. To examine the importance of p53 in mediating this arrest, mouse embryo fibroblasts that are either wild-type or that are defective in p53 were also subjected to hypothermia. Only those cells with wild-type p53 undergo a cell cycle arrest, indicating that p53 has a role in mediating this response. Because many tumor cells have defective p53, this suggests that hypothermia may increase the selective toxicity of chemotherapeutic agents for tumor cells.
Matijasevic
Z
Z
Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester 01655-0126, USA.
Snyder
J E
JE
Ludlum
D B
DB
D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007035:Hypothermia; D051379:Mice; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53