title: Tissue expression of the vesicle protein pantophysin.
abstract: The cell-type restricted expression of cytoplasmic microvesicle membrane protein isoforms may be a consequence of the functional adaptation of these vesicles to the execution of specialized processes in cells of different specialization. To characterize the expression of the vesicle protein pantophysin, an isoform of the synaptic vesicle proteins synaptophysin and synaptoporin, we have prepared and characterized antibodies useful for the immunological detection of pantophysin in vitro and in situ. Using these reagents, we show by immunoblot analyses that pantophysin expression is not homogeneous but differs significantly between various bovine tissues. Furthermore, these differences are not exactly paralleled by the expression of other vesicle proteins of the SCAMP (secretory carrier-associated membrane protein) and VAMP (vesicle-associated membrane protein) types that have previously been localized to pantophysin vesicles in cultured cells. By immunofluorescence microscopy, pantophysin expression is seen predominantly in non-neuroendocrine cells with pronounced membrane traffic. Pantophysin staining codistributes with SCAMP and VAMP immunoreactivities in most instances but differs in some. Remarkably, pantophysin staining in liver is restricted to cells surrounding sinusoids and is not detectable in hepatocytes, similar to that of the SCAMP and VAMP isoforms as detected by our reagents in tissue sections.
authors.affiliation: Department of Anatomy, Johannes Gutenberg-University Mainz, Becherweg 13, D-55099 Mainz, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Windoffer
authors.forename: M
authors.initials: M
authors.lastname: Borchert-Stuhlträger
authors.forename: N K
authors.initials: NK
authors.lastname: Haass
authors.forename: S
authors.initials: S
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Hergt
authors.forename: C J
authors.initials: CJ
authors.lastname: Bulitta
authors.forename: R E
authors.initials: RE
authors.lastname: Leube
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D002417:Cattle; D003594:Cytoplasmic Granules / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000096:biosynthesis; D009928:Organ Specificity; D020033:Protein Isoforms / Q000096:biosynthesis; D050683:R-SNARE Proteins
chemical_list: D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D050683:R-SNARE Proteins