title: Shiga toxin-producing Escherichia coli O157 in feedlot cattle and Norwegian rats from a large-scale farm.
abstract: A total of 365 faecal samples from different categories of cattle, 12 samples of untreated slurry, 50 samples of fresh droppings of feral domestic pigeons, 20 samples of fresh droppings of domestic sparrows and stool samples of 19 synanthropic rodents were examined for the presence of Escherichia coli by broth enrichment culture and a subsequent immunomagnetic separation. Escherichia coli O157 was found in 72 (20%) bovine samples, six (50%) samples of untreated slurry and four (40%) of 10 rats (Rattus norvegicus). Significant differences were found in the E. coli O157 shedding frequency between different age categories of bulls. Genes stx2 and eaeA were detected in all isolates, and the stx1 gene in all but 10 isolates.
authors.affiliation: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. cizekal@post.cz
authors.forename: A
authors.initials: A
authors.lastname: Cízek
authors.forename: P
authors.initials: P
authors.lastname: Alexa
authors.forename: I
authors.initials: I
authors.lastname: Literák
authors.forename: J
authors.initials: J
authors.lastname: Hamrík
authors.forename: P
authors.initials: P
authors.lastname: Novák
authors.forename: J
authors.initials: J
authors.lastname: Smola
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D010856:Columbidae / Q000382:microbiology; D003470:Culture Media; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D018189:Immunomagnetic Separation; D051381:Rats / Q000382:microbiology*; D012376:Rodent Diseases / Q000382:microbiology; D022603:Shiga Toxins; D020308:Songbirds / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media; D022603:Shiga Toxins