An easy colorimetric assay for screening and qualitative assessment of deiodination and dehalogenation by bacterial cultures.
Halogenated organic substances are among the main environmental concerns. A number of micro-organisms are able to dehalogenate these compounds. However, the methods for the assessment of micro-organismal ability to dehalogenate are expensive and require complex instrumentation. Here, an easy colorimetric assay for the screening and assessment of the ability of bacterial cultures to deiodinate, and potentially dehalogenate, chemical substances is proposed. The method is based on the oxidation of iodide, released due to biotransformation, to iodine followed by a subsequent detection of iodine by a classical reaction with starch.
Kuritz
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Oak Ridge National Laboratory, TN 37831, USA. kuritzt@ornl.gov
D017033:Anabaena / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D001673:Biodegradation, Environmental; D002723:Chlorobenzoates / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007463:Iodobenzoates / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics
D002723:Chlorobenzoates; D007463:Iodobenzoates; C040768:4-chlorobenzoic acid