Mitochondrial participation in modulation of calcium transients in DRG neurons.
The effects of the mitochondrial Na+/Ca2+ exchange blocker tetraphenylphosphonium (TPP+) and the permeability transition blocker cyclosporinA (CysA) on the ability of mitochondria to participate in the regulation of intracellular calcium were investigated on freshly isolated mice sensory DRG neurons. The free intracellular calcium level ([Ca2+]in) was measured using indo-1 based microfluorimetry. The characteristics of depolarization-induced [Ca2+]in transients were changed in the presence of 25 microM TPP+. The amplitude of [Ca2+]in transients became decreased and the restoration of resting [Ca2+]in level speeded up in the presence of TPP+. Application of 5 microM cyclosporinA induced substantial residual elevation of [Ca2+]in after termination of depolarization. We conclude that the mitochondrial Na+/Ca2+ exchanger mechanism plays an important role in the regulation of calcium signals during neuronal activity, prolonging them by releasing Ca2+ stored during transient peak. Activation of permeability transition pores does not participate in these processes.
Svichar
N
N
Bogomoletz Institute of Physiology, Kiev, Ukraine.
Shishkin
V
V
Kostyuk
P
P
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000502:physiology*; D006706:Homeostasis; D066298:In Vitro Techniques; D007424:Intracellular Fluid; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009861:Onium Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology
D009861:Onium Compounds; D009943:Organophosphorus Compounds; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D011189:Potassium Chloride; D016572:Cyclosporine; D002118:Calcium; C013289:tetraphenylphosphonium