[Dynamic changes of nitric oxide and biochemistry in rats with intrahepatic cholestasis].
OBJECTIVE
To investigate the correlation between nitric oxide and biochemical changes of intrahepatic cholestasis.


METHODS
Studies were made on endogenous NO, biochemical changes of cholestasis, liver mitochondrial function and renal excretion in a rat model at different time points after ANIT administration.


RESULTS
In acute reactive phase, serum bilirubin, ALT, AKP, and bile acids significantly increased, accompanied by enhanced plasma NO and renal excretion. Liver NO increased and mitochondrial SDH activity decreased for 2-6 days after ANIT. There was a high negative correlation between liver NO and mitochondrial SDH activity (r = -0.92).


CONCLUSIONS
These results show that higher liver NO may be an important mediator of intrahepatic cholestasis. However, plasma NO increase may play a role in rapidly decreasing serum bilirubin and restoring bile acids, ALT, and AKP to control levels by mediating enhanced renal excretion.
Pei
Q
Q
Dalian Medical University.
Wang
D
D
Jiang
M
M
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D002780:Cholestasis, Intrahepatic / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000378:metabolism
D009569:Nitric Oxide; D013385:Succinate Dehydrogenase; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin