Cloning and physical mapping of Atlantic salmon (Salmo salar L.) telomeric sequences.
We cloned and sequenced DNA containing telomeric sequences from Atlantic salmon (Salmo salar L.). At both low and high stringency conditions, a cloned probe containing (TTAGGG)n sequences binds only at the extreme termini of the chromosomes, not at interstitial sites. Blocks of interstitial telomere-like sequences were expected from the chromosome polymorphism because of Robertsonian translocations that exist in Atlantic salmon. Our results support the hypothesis that telomeric sequences were almost entirely lost during chromosome fusion events.
Perez
J
J
Departamento Biologia Funcional, Universidad de Oviedo, Facultad de Medicina, C/Julian Claveria, s/n. 33006-Oviedo, Spain.
Moran
P
P
Garcia-Vazquez
E
E
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D020161:Physical Chromosome Mapping; D012091:Repetitive Sequences, Nucleic Acid; D020317:Salmo salar / Q000235:genetics*; D016615:Telomere / Q000235:genetics*
D015345:Oligonucleotide Probes