title: Transregulation of erbB expression in the mouse olfactory bulb.
abstract: Previously, we have shown that erbB-3 expression is restricted to the ensheathing cells of the olfactory nerve layer, while erbB-4 is found in the periglomerular and mitral/tufted cells of the olfactory bulb and in cells coming out from the rostral migratory stream of the subependymal layer. In the present work, we have treated adult mice with zinc sulfate intranasal irrigation and analyzed erbB-3 and erbB-4 expression in the deafferented olfactory bulb. Following treatment, olfactory axons undergo degeneration, as indicated by the loss of OMP expression in the deafferented olfactory bulb. The thickness of the olfactory nerve layer is reduced, but the specific intensity of erbB-3 labeling in the remaining olfactory nerve layer is increased with respect to control. Interestingly, following deafferentation, erbB-4 immunoreactivity decreases specifically in cell types that normally make synaptic contacts with primary olfactory neurons in the glomeruli, i.e. periglomerular and mitral/tufted cells. Partial lesion of the olfactory epithelium allows regenerative axon growth of olfactory neurons to the olfactory bulb. Following olfactory axon regeneration, erbB-3 and erbB-4 immunoreactivity in the olfactory bulb is similar to control. Thus, like tyrosine hydroxylase, the down regulation of erbB-4 expression in the periglomerular cells is reversible.
authors.affiliation: Dipartimento di Biologia Animale e dell'Uomo, Università degli Studi di Torino, Italy. perroteau@dm.unito.it
authors.forename: I
authors.initials: I
authors.lastname: Perroteau
authors.forename: M
authors.initials: M
authors.lastname: Oberto
authors.forename: I
authors.initials: I
authors.lastname: Soncin
authors.forename: S
authors.initials: S
authors.lastname: Voyron
authors.forename: M
authors.initials: M
authors.lastname: De Bortoli
authors.forename: P
authors.initials: P
authors.lastname: Bovolin
authors.forename: A
authors.initials: A
authors.lastname: Fasolo
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D009410:Nerve Degeneration / Q000139:chemically induced; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000187:drug effects; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism*; D051842:Olfactory Marker Protein; D009832:Olfactory Nerve / Q000187:drug effects / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020893:Receptor, ErbB-3; D066247:Receptor, ErbB-4; D019287:Zinc Sulfate
chemical_list: D009419:Nerve Tissue Proteins; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D011518:Proto-Oncogene Proteins; D019287:Zinc Sulfate; D066246:ErbB Receptors; C587690:Erbb4 protein, mouse; D020893:Receptor, ErbB-3; D066247:Receptor, ErbB-4