Fresh vs aged benzylpenicillin on non-IgE responses in mice.
OBJECTIVE
To study whether or not the freshly prepared benzylpenicillin could induce different non-IgE antibody response from aged benzylpenicillin.


METHODS
Antibody response was determined by enzyme-linked immunosorbent assay (ELISA). Antigen molecules recognized by antibodies and antigenic cross reactions were tested by hapten inhibition assay.


RESULTS
Isotypes of specific non-IgE antibodies induced by freshly prepared benzylpenicillin were mainly IgM, and then IgG and IgA. Some parts of specific antibodies recognized benzylpenicillin molecule and major parts combined with degraded or transforming products. Isotypes of antibodies responsible for cross reaction were mainly IgG between benzylpenicillin and ampicillin and IgM between benzylpenicillin and piperacillin.


CONCLUSIONS
Freshly prepared and aged benzylpenicillin induced different non-IgE antibody response.
Zhang
L
L
Department of Pharmacology, School of Pharmacy, Shanghai Medical University, China.
Chen
B Y
BY
Shan
B Z
BZ
Yu
L M
LM
Chen
B J
BJ
Wang
Y M
YM
D000667:Ampicillin / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D010400:Penicillin G / Q000276:immunology*; D010406:Penicillins / Q000276:immunology*; D010878:Piperacillin / Q000276:immunology; D013997:Time Factors
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010406:Penicillins; D000667:Ampicillin; D010400:Penicillin G; D010878:Piperacillin