Detection of glycosidic residues in carpal glands of wild and domestic pig revealed by basic and lectin histochemistry.
Carpal glands are compound tubuloalveolar glands, located on the medial surface of the carpus. This study was carried out on samples from carpal glands of adult wild and domestic pigs of both sexes. We elucidated the glycosidic composition of carpal gland secretion in situ using traditional histochemical methods and lectin histochemistry. Some secretory cells exhibited an intense reaction with PAS in both wild and domestic pigs. Lectin histochemistry showed differences in the localization and composition of glycoconjugates secreted by carpal glands. A cytoplasmic positivity was revealed in the wild pig by the sequence sialidase-PNA and WGA, whereas in the domestic pig the reactivity was localized at the apical surface of some cells. LTA positive cells were found only in the carpal glands of the domestic pig.
Pedini
V
V
Istituto di Anatomia degli Animali Domestici, Facoltà di Medicina Veterinaria, Perugia, Italy.
Scocco
P
P
Dall'Aglio
C
C
Gargiulo
A M
AM
D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D005260:Female; D006001:Glycoconjugates / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D037102:Lectins; D008297:Male; D009439:Neuraminidase; D012543:Scent Glands / Q000166:cytology* / Q000378:metabolism; D013545:Sweat Glands / Q000166:cytology* / Q000378:metabolism; D013552:Swine / Q000033:anatomy & histology*
D006001:Glycoconjugates; D006027:Glycosides; D037102:Lectins; D009439:Neuraminidase