Cytogenetic and CGH studies of four neuroendocrine tumors and tumor-derived cell lines of a patient with multiple endocrine neoplasia type 1.
A malignant insulinoma (LOHG-I), a carcinoid of the lung (LOHG-L), a parathyroid adenoma (LOHG-NSA), and a fibroma (LOHG-F) were obtained from a patient with multiple endocrine neoplasia type 1 (MEN1). Long-term cultures were established. Essential neurobiological properties of the cell lines were proven immunocytochemically and by electron microscopy. Molecular analysis of the germline DNA showed a 4 bp deletion in exon 3 of the MEN1 gene. Cytogenetic and CGH analyses of the tumors/tumor cell lines revealed diploidy and balanced and unbalanced structural aberrations different for each tumor. Chromosomes 6q21, 11q and 17q were most frequently involved in clonal structural aberrations.
Sigl
E
E
Department of General and Experimental Pathology, University of Graz, Graz, Austria.
Behmel
A
A
Henn
T
T
Wirnsberger
G
G
Weinhausl
A
A
Kaserer
K
K
Niederle
B
B
Pfragner
R
R
D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000310:Adrenal Gland Neoplasms / Q000473:pathology; D000328:Adult; D000782:Aneuploidy; D002276:Carcinoid Tumor / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D004938:Esophageal Neoplasms / Q000473:pathology; D005350:Fibroma / Q000235:genetics* / Q000473:pathology; D016147:Genes, Tumor Suppressor; D006801:Humans; D007340:Insulinoma / Q000235:genetics* / Q000473:pathology; D007889:Leiomyoma / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D010282:Parathyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D010673:Pheochromocytoma / Q000473:pathology; D011518:Proto-Oncogene Proteins; D017384:Sequence Deletion; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; C105373:MEN1 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins