Establishment and characterization of human laryngeal squamous cell carcinoma cell lines.
OBJECTIVE
Six human laryngeal squamous cell carcinoma cell lines (SNU-46, -585, -899, -1066, -1076, -1214) established from Korean patients are reported.


METHODS
In vitro culture of six squamous cell carcinoma cell lines derived from primary tumors of the larynx. Description of the cell line phenotypes and determination of molecular characteristics.


METHODS
Six laryngeal squamous cell carcinoma cell lines were cultured. The cell phenotypes, including the histopathology of the primary tumors and in vitro growth characteristics, were determined. Molecular characterization was also performed, including DNA fingerprinting analysis and abnormalities of p15, p16, p53, and TGF-betaRII genes by polymerase chain reaction-based single strand conformation polymorphism and sequencing analysis.


RESULTS
All cell lines grew as adherent cells; five lines grew as monolayers and one other line grew as stratifying colonies. All lines showed 1) high viability (75%-92%) with various doubling times (36-96 h); 2) absence of Mycoplasma and other bacteria; and 3) genetic heterogeneity by DNA profile analysis. p53 Mutations were found in three lines and p16 mutations were observed in five cell lines. TGF-betaRII mutations were found in two lines: one line had frameshift mutation and another line had a missense mutation at the kinase domain.


CONCLUSIONS
These newly established and characterized laryngeal squamous cell carcinoma cell lines will be useful for investigating the biologic characteristics of laryngeal cancer.
Ku
J L
JL
Laboratory of Cell Biology, Cancer Research Institute, Seoul National University College of Medicine, Korea.
Kim
W H
WH
Lee
J H
JH
Park
H S
HS
Kim
K H
KH
Sung
M W
MW
Park
J G
JG
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D016172:DNA Fingerprinting; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000302:isolation & purification; D016147:Genes, Tumor Suppressor / Q000235:genetics; D019942:Genes, p16 / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018895:Microsatellite Repeats; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D012334:RNA, Neoplasm / Q000302:isolation & purification; D016212:Transforming Growth Factor beta / Q000235:genetics; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D016212:Transforming Growth Factor beta