title: Characterization of elicitin-like phospholipases isolated from Phytophthora capsici culture filtrate.
abstract: The phytopathogenic oomycete Phytophthora capsici secretes in culture a phospholipase activity. Two enzyme isoforms exhibiting a high phospholipase B activity were isolated by chromatography and electrophoresis. They differ in their apparent molar masses (22 and 32 kDa). Both proteins are glycosylated and share the same N-terminal amino acid sequence up to the 39th residue with a high homology with capsicein, the P. capsici elicitin. Although devoid of phospholipase activity, capsicein was shown by circular dichroism to specifically interact with negatively charged phospholipids, suggesting that the membrane lipids could be a potential target for elicitins.
authors.affiliation: Unité de Recherches de Biochimie et Structure des Protéines, INRA, Jouy-en-Josas, France. nespoulo@jouy.inra.fr
authors.forename: C
authors.initials: C
authors.lastname: Nespoulous
authors.forename: O
authors.initials: O
authors.lastname: Gaudemer
authors.forename: J C
authors.initials: JC
authors.lastname: Huet
authors.forename: J C
authors.initials: JC
authors.lastname: Pernollet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002211:Capsaicin / Q000737:chemistry; D002417:Cattle; D002942:Circular Dichroism; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008245:Lysophospholipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry; D010740:Phospholipases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D010838:Phytophthora / Q000201:enzymology*; D018515:Plant Leaves; D010947:Plants, Toxic; D011487:Protein Conformation; D016415:Sequence Alignment; D014026:Nicotiana
chemical_list: D005656:Fungal Proteins; D007527:Isoenzymes; D010446:Peptide Fragments; D010740:Phospholipases; D010741:Phospholipases A; D008245:Lysophospholipase; D002211:Capsaicin