title: Role of cholecystokinin in the anorexia produced by duodenal delivery of peptone in rats.
abstract: We used the cholecystokinin receptor antagonist devazepide to assess the importance of CCK in mediating the anorexia produced by 2-h duodenal infusions of peptone, a protein digest, at dark onset in nonfasted rats. Peptone alone (0.14-2.24 g/h) suppressed food intake dose dependently by 18-96%, with an approximate half-maximal dose of 1 g/h. Peptone-induced reductions in caloric ingestion were comparable to the caloric loads infused. Devazepide alone (30-1,000 microgram/kg) stimulated food intake dose dependently by 30-73%, with a minimal effective dose of 100 micrograms/kg. Devazepide appeared to reverse the anorexic response to peptone (1.1 g/h) dose dependently by 29-65%, with a minimal effective dose of 30 micrograms/kg. The magnitudes of these devazepide-induced effects were similar to, and in some cases were larger than, those produced when the same doses of devazepide were administered alone. Coadministration of devazepide (1,000 micrograms/kg) and a lower peptone dose (0.8 g/h) produced similar results. These results suggest that an essential CCK mechanism plays a significant role in mediating the satiety response to duodenal delivery of protein.
authors.affiliation: Veterans Administration Medical Center, Omaha 68105; and Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska 68178, USA.
authors.forename: T
authors.initials: T
authors.lastname: Woltman
authors.forename: R
authors.initials: R
authors.lastname: Reidelberger
mesh_terms: D000818:Animals; D000855:Anorexia / Q000139:chemically induced* / Q000503:physiopathology*; D002766:Cholecystokinin / Q000502:physiology*; D020109:Devazepide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000502:physiology; D004435:Eating / Q000187:drug effects / Q000502:physiology; D006727:Hormone Antagonists / Q000494:pharmacology; D007267:Injections; D008297:Male; D010461:Peptones / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors
chemical_list: D006727:Hormone Antagonists; D010461:Peptones; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin; D020109:Devazepide