[Cloning and expression of the gene for the mycoplasma key division protein FtsZ in Escherichia coli].
Gene ftsZ responsible for division of bacterial cells was revealed in most prokaryote groups. A 520-bp fragment of the ftsZ gene was amplified on the template of A. laidlawii DNA using degenerate primers. This fragment was sequenced and served as a hybridization probe for cloning of the full-sized copy of the A. laidlawii ftsZ gene. The amplified fragment was cloned in a pGEX3X vector and expressed in E. coli cells. Polyclonal antibodies derived from the chimeric polypeptide containing a fragment of A. laidlawii FtsZ protein interacted only with the A. laidlawii protein with molecular mass of 40 kDa. Comparison of nucleotide sequences of the ftsZ-gene region of A. laidlawii and other bacterial species showed that they were highly homologous in A. laidlawii, E. coli, and Bac. subtilis, while low homology was revealed between the A. laidlawii sequence and those of the members of the genus Mycoplasma. Analysis of the ftsZ-gene nucleotide sequences is suggested as a means to study the evolutionary relatedness of prokaryotes.
Kukekova
A V
AV
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Malinin
A Iu
AIu
Vonskiĭ
M A
MA
Borkhsenius
S N
SN
D000128:Acholeplasma laidlawii / Q000235:genetics*; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics*; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D000939:Epitopes; C071525:FtsZ protein, Bacteria
Klonirovanie i ĕkspressiia gena kliuchevogo belka deleniia FtsZ mikoplazmy v Escherichia coli.