[The levels and nature of translation in mouse preimplantation embryos with repressed cytokinesis].
The translational activity of embryos cultured up to the 8-cell stage in cytochalasin D-supplemented medium was studied. These embryos remained unicellular during the entire preimplantation period. Blastocoel formation and hatching began in the cytochalasin-treated embryos at the same time as in the control. We have studied the overall translation rates of "one-cell embryos" and estimated the relative translational differences for individual polypeptides compared to the control embryos. Up to the early blastocyst stage, the translation levels in these embryos, measured by 35S-methionine incorporation, were about two times lower than in the control. At later stages, the differences were fivefold. The ppm values of approximately one-third of all individual spots on two-dimensional electrophoregrams differed three times or more between the control and cytochalasin-treated embryos. These results suggest that the first morphogenetic events in mammalian ontogenesis may be controlled autonomously and the timing of morphogenetic transitions is controlled by acquisition of a definite set of stage-specific factors which serves as the signal for subsequent development.
Evsikov
A V
AV
Department of Biochemical Genetics, National Academy of Sciences of Ukraine, Kiev.
Solomko
A P
AP
D000818:Animals; D001755:Blastocyst / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques / Q000379:methods; D015638:Cytochalasin D / Q000494:pharmacology; D003864:Depression, Chemical; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D051379:Mice; D008815:Mice, Inbred Strains; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*
D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D
Urovni i kharakter transliatsii u doimplantatsionnykh zarodysheĭ myshi s podavlennym tsitokinezom.