Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale.
We report the luminescence and spectral properties of a phospholipid analogue containing a long-lifetime luminescent rhenium metal-ligand complex (MLC) covalently linked to the amino group of phosphatidyl ethanolamine. When incorporated into synthetic membranes, this lipid probe displays intensity decay times near 3 microseconds. Importantly, the probe displays highly polarized emission with a maximal fundamental anisotropy of 0.33. This probe is expected to have numerous applications for studies of microsecond diffusion and dynamics of membranes.
Li
L
L
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Castellano
F N
FN
Gryczynski
I
I
Lakowicz
J R
JR
D004058:Diffusion; D008024:Ligands; D008563:Membrane Lipids / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D012211:Rhenium; D013053:Spectrophotometry; D013816:Thermodynamics; D013997:Time Factors
D008024:Ligands; D008563:Membrane Lipids; D015335:Molecular Probes; D009942:Organometallic Compounds; D010714:Phosphatidylethanolamines; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D012211:Rhenium