title: Enhancement of endothelial cell E-selectin expression by sera from patients with active Behçet's disease: moderate correlation with anti-endothelial cell antibodies and serum myeloperoxidase levels.
abstract: We studied the in vitro E-selection expression of endothelial cells treated with sera from patients with Behçet's disease (BD) and factors (anti-endothelial cell antibodies, anti-neutrophil cytoplasmic antibodies, cytokines, and myeloperoxidase (MPO) that may contribute to adhesion molecule expression. A total of 21 patients with BD and 27 healthy controls were studied. In vitro E-selectin endothelial cell expression was investigated by ELISA after HUVEC incubation with sera or purified IgG from patients with BD and controls. Increased E-selectin expression was observed when endothelial cells were incubated with sera from patients with active disease or from patients with circulating anti-endothelial cell antibodies and high levels of plasma myeloperoxidase. Abnormalities of endothelial cell function have been suggested to play a role in the occurrence of vascular damage in BD. Our findings suggest that anti-endothelial cell antibodies and neutrophil hyperactivity, as inferred from the high plasma MPO levels in patients with active disease, may explain endothelial cell activation and neutrophil accumulation in vascular lesions.
authors.affiliation: Cattedra di Allergologia e Immunologia Clinica, University of Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Triolo
authors.forename: A
authors.initials: A
authors.lastname: Accardo-Palumbo
authors.forename: G
authors.initials: G
authors.lastname: Triolo
authors.forename: M C
authors.initials: MC
authors.lastname: Carbone
authors.forename: A
authors.initials: A
authors.lastname: Ferrante
authors.forename: E
authors.initials: E
authors.lastname: Giardina
mesh_terms: D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D001528:Behcet Syndrome / Q000097:blood* / Q000201:enzymology / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D019040:E-Selectin / Q000097:blood / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000097:blood* / Q000276:immunology
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D016207:Cytokines; D019040:E-Selectin; D007074:Immunoglobulin G; C087589:anti-endothelial cell antibody; D009195:Peroxidase