Biotransformation of pentachlorophenol, aniline and biphenyl in isolated rainbow trout (Oncorhynchus mykiss) hepatocytes: comparison with in vivo metabolism.
1. The biotransformation of pentachlorophenol (PCP), aniline and biphenyl in rainbow trout (Oncorhynchus mykiss) isolated liver cells was investigated to examine if fish hepatocytes represent a suitable alternative to the in vivo approach for studying the biotransformation of chemicals. Each compound was incubated at two concentrations (10 and 60 microM) for 2 h. For comparison, the metabolic profile of these xenobiotics was also studied in urine and bile of trout orally exposed to 1.8-4.0 mg/kg wet wt of each compound. 2. In vitro as in vivo, PCP glucuronide and to a lesser extent PCP sulphate were the metabolites formed by trout from PCP. 3. Aniline was mainly metabolized to acetanilide and to a lesser extent to 2-aminophenol by isolated hepatocytes, but neither hydroxylated acetanilide nor conjugates were found in vitro whereas they were present in bile and urine of trout treated with this chemical. 4. Trout hepatocytes metabolized biphenyl to hydroxylated and dihydroxylated products and the corresponding glucuronides. These results correlated well with the metabolic profile obtained from the bile of trout exposed to this pesticide. 5. It is concluded that although hepatocytes are well suited for several types of biotransformation studies, the fact that this system may in some cases produce a different metabolic pattern than in vivo should be considered when attempting to extrapolate in vitro to in vivo data.
Cravedi
J P
JP
Laboratoire des Xénobiotiques, INRA, Toulouse, France. jcravedi@toulouse.inra.fr
Lafuente
A
A
Baradat
M
M
Hillenweck
A
A
Perdu-Durand
E
E
D000814:Aniline Compounds / Q000493:pharmacokinetics* / Q000652:urine; D000818:Animals; D001711:Biotransformation; D001713:Biphenyl Compounds / Q000493:pharmacokinetics* / Q000652:urine; D002273:Carcinogens / Q000493:pharmacokinetics; D005260:Female; D005659:Fungicides, Industrial / Q000493:pharmacokinetics / Q000652:urine; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010416:Pentachlorophenol / Q000493:pharmacokinetics* / Q000652:urine; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics
D000814:Aniline Compounds; D001713:Biphenyl Compounds; D002273:Carcinogens; D005659:Fungicides, Industrial; D010416:Pentachlorophenol; D014874:Water Pollutants, Chemical; C010574:biphenyl; C023650:aniline