Relationship of human pancreatic cholesterol esterase gene structure with lipid phenotypes.
Pancreatic cholesterol esterase is one of the enzymes that plays a pivotal role in cholesterol absorption. Differences in the genotype of this enzyme could affect the susceptibility of individuals to dyslipidemia and/or cardiovascular disease. We undertook this study to investigate if any correlation exists between restriction fragment length polymorphism in the human pancreatic cholesterol esterase gene and serum lipid levels. DNA from 96 healthy adults was restricted with Stu I, Southern blotted, and probed with cDNA of human pancreatic cholesterol esterase. Results revealed six distinct patterns which were classified as A, B, C, D, E, and F which had a population frequency of 1%, 34.5%, 49%, 12.5%, 1% and 2% respectively. Correlation of the distribution of lipid and lipoprotein levels by pattern and sex revealed a significant interaction between pattern type and HDL (p=0.03) in the most common group (group C) for males. Male patients of pattern C tended to have a lower LDL cholesterol than non-pattern C males (p=0.07); in addition, 80% of all males in the study population with LDL cholesterol under 100 mg/dl were found in pattern C. Thus, the most common Stu I RFLP genotype is associated with a favorable lipid phenotype. This report shows an association between the human pancreatic cholesterol esterase genotype and serum lipid levels. Further analysis of a larger study group with Stu I and alternative polymorphic restriction enzymes is warranted, to confirm this biologically plausible result.
Aleman-Gomez
J A
JA
Department of Medicine, Washington University Medical Center, St. Louis, Missouri, USA.
Colwell
N S
NS
Vyas
K
K
Borecki
I
I
Shonfeld
G
G
Lange
L G
LG
Kumar
V B
VB
D000328:Adult; D002784:Cholesterol / Q000097:blood; D004247:DNA / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D002787:Sterol Esterase / Q000235:genetics*
D008055:Lipids; D008074:Lipoproteins; D004247:DNA; D002784:Cholesterol; D002787:Sterol Esterase