title: Embryonic hemoglobins are expressed in definitive cells.
abstract: Human embryonic zeta and epsilon globin chains are synthesized in yolk sac-derived primitive erythroid cells, and decrease rapidly during definitive erythropoiesis. Examination of zeta and epsilon globin expression at the cellular level using dual-color immunofluorescence staining with specific monoclonal antibodies showed that embryonic globin proteins are present in definitive erythroid cells. More than half of fetal erythrocytes were positive for zeta and approximately 5% for epsilon globin. Approximately one third of newborn red blood cells were zeta-positive and less than 1% epsilon-positive. Adult erythrocytes did not have embryonic globins. Erythroblasts that developed in liquid cultures also contained embryonic globin in amounts which declined with ontogenic age, and the proportion of positive cells in vitro was less than in the comparable erythrocytes that developed in vivo. Thus, embryonic globin chains are synthesized in definitive erythroid cells and decrease with ontogeny. Modulation of embryonic globin gene expression is not solely due to a switch from primitive to definitive erythropoiesis.
authors.affiliation: Division of Pediatric Hematology/Oncology, University of Texas Medical Branch, Galveston, Texas 77555-0361, USA.
authors.forename: H Y
authors.initials: HY
authors.lastname: Luo
authors.forename: X L
authors.initials: XL
authors.lastname: Liang
authors.forename: C
authors.initials: C
authors.lastname: Frye
authors.forename: M
authors.initials: M
authors.lastname: Wonio
authors.forename: G D
authors.initials: GD
authors.lastname: Hankins
authors.forename: D H
authors.initials: DH
authors.lastname: Chui
authors.forename: B P
authors.initials: BP
authors.lastname: Alter
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn
chemical_list: D005319:Fetal Hemoglobin