title: Selenium-enriched Agaricus bisporus mushrooms suppress 7,12-dimethlybenz[a]anthracene bioactivation in mammary tissue.
abstract: The present studies compared dietary Se (1.0 microg/g) when provided as either fortified Agaricus bisporus mushrooms, or sodium selenite on the in vivo metabolism of 7,12-dimethylbenz(a)anthracene (DMBA). Dietary addition of Se unenriched A. bisporus mushrooms at 2% did not alter the occurrence of DMBA induced DNA adducts or the activity of glutathione S-transferase (GST). However, the addition of Se as enriched mushrooms, or as selenite, significantly increased both liver and mammary GST activity. Providing sodium selenite, or enriched mushrooms also significantly reduced total and anti-3,4-dihydrodiol-1,2-epoxide-deoxyguanosine adducts compared to feeding the basal diet (P < 0.05). These investigations provide evidence that Se enriched mushrooms can be used as an effective method to retard chemically induced tumors.
authors.affiliation: Department of Food Science, The Pennsylvania State University, University Park, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Spolar
authors.forename: E M
authors.initials: EM
authors.lastname: Schaffer
authors.forename: R B
authors.initials: RB
authors.lastname: Beelman
authors.forename: J A
authors.initials: JA
authors.lastname: Milner
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000493:pharmacokinetics*; D000363:Agaricales; D000818:Animals; D001711:Biotransformation; D002273:Carcinogens / Q000493:pharmacokinetics*; D018736:DNA Adducts / Q000032:analysis; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000008:administration & dosage*
chemical_list: D002273:Carcinogens; D018736:DNA Adducts; D015127:9,10-Dimethyl-1,2-benzanthracene; D005982:Glutathione Transferase; D012643:Selenium