title: [Basic calponin expressing human neuroblastoma cell line of KP-N-YS].
abstract: Recent studies have demonstrated that human neuroblastoma (NB) cell lines have at least two morphological appearance of neuroblastic (N-type) and substrate-adhesive (S-type) cells. Our previous study revealed that S-type cells expressed alpha-smooth muscle actin and/or desmin, suggesting the smooth muscle cell characteristics of S-type cells. In the present study, a new human NB cell line, KP-N-YS, was established from bone marrow metastasis of a four-year-old boy with advanced NB, originating from the left adrenal gland. Subsequently N-type cell clone (YS1n) and S-type cell clone (YS2s) was isolated from the parent cell line. Parent and clones cell lines were identified as NB by surface membrane antigen and cytoskeletal protein analysis, and these cell lines were demonstrated as common progenitors by chromosomal analysis. Furthermore the presence of basic calponin were determined by indirect immunofluorescence, Western blot, as well as by RT-PCR (reverse transcriptase-polymerase chain reaction). Our demonstration of basic calponin not in N-type cells, but in S-type cells supports the plausible smooth muscle cell characteristics of this NB cell line.
authors.affiliation: Department of Pediatrics, Kyoto Prefectural University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yagi
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
authors.forename: H
authors.initials: H
authors.lastname: Mine
authors.forename: Y
authors.initials: Y
authors.lastname: Horii
authors.forename: T
authors.initials: T
authors.lastname: Matsumura
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: J
authors.initials: J
authors.lastname: Goshi
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002675:Child, Preschool; D002999:Clone Cells; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008840:Microfilament Proteins; D009130:Muscle, Smooth / Q000166:cytology; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D000096985:Calponins
chemical_list: D000954:Antigens, Surface; D002135:Calcium-Binding Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D000096985:Calponins