title: The effect of Helicobacter pylori on neutrophil chemotaxis is independent of cagA.
abstract: Sixteen Helicobacter pylori strains were studied in order to determine their neutrophil chemotactic activity and the association with the presence cagA gene. Neutrophil chemotactic activity was detected by a modified Boyden chamber method and the results were expressed in terms of chemotactic index (CI). The presence of cagA was determined by PCR. Of the 16 strains, eight were cagA+ and eight were cagA-. All of the isolated strains showed chemotactic activity. The mean value of CI of the patient group was significantly higher than the negative control (P < 0.01). The mean value of CI of zymosan-activated serum (P < 0.05) and the reference strain H. pylori NCTC 11637 (HP11637) (P < 0.01) was significantly higher than the patient group's mean value of CI. There were no statistical significance in the CI between cagA+ and cagA- strains (P > 0.05). It is concluded that H. pylori attracts neutrophils by chemotaxis, however, there is no association with cagA.
authors.affiliation: Hacettepe University, Medical Faculty, Department of Clinical Microbiology, Ankara, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Akyön
authors.forename: G
authors.initials: G
authors.lastname: Hascelik
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D004381:Duodenal Ulcer / Q000276:immunology / Q000382:microbiology; D005260:Female; D005756:Gastritis / Q000276:immunology / Q000382:microbiology; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori