Caffeine interaction with fluorescent calcium indicator dyes.
We report that caffeine, in millimolar concentrations, interacts strongly with four common calcium indicator dyes: mag-fura-2, magnesium green, fura-2, and fluo-3. Fluorescence intensities are either noticeably enhanced (mag-fura-2, fura-2) or diminished (magnesium green, fluo-3). The caffeine-induced changes in the fluorescence spectra are clearly distinct from those of metal ion binding at the indicator chelation sites. Binding affinities for calcium of either mag-fura-2 or magnesium green increased only slightly in the presence of caffeine. Caffeine also alters the fluorescence intensities of two other fluorescent dyes lacking a chelation site, fluorescein and sulforhodamine 101, implicating the fluorophore itself as the interaction site for caffeine. In the absence of caffeine, variation of solution hydrophobicity by means of water/dioxane mixtures yielded results similar to those for caffeine. These observations suggest that hydrophobic substances, in general, can alter dye fluorescence in a dye-specific manner. For the particular case of caffeine, and perhaps other commonly used pharmacological agents, the dye interactions can seriously distort fluorescence measurements of intracellular ion concentrations with metal indicator dyes.
Muschol
M
M
Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6074 USA.
Dasgupta
B R
BR
Salzberg
B M
BM
D000814:Aniline Compounds / Q000737:chemistry; D002110:Caffeine / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D004146:Dioxanes / Q000737:chemistry; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D016257:Fura-2 / Q000031:analogs & derivatives / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013805:Theobromine / Q000737:chemistry; D014966:Xanthenes / Q000737:chemistry
D000814:Aniline Compounds; D002614:Chelating Agents; D004146:Dioxanes; D005456:Fluorescent Dyes; D012235:Rhodamines; D014966:Xanthenes; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; C059715:Fluo-3; D002110:Caffeine; C050829:sulforhodamine 101; C025223:1,4-dioxane; D013805:Theobromine; D002118:Calcium; D019793:Fluorescein; D016257:Fura-2