Detection of specific antibodies against human cultured chondrosarcoma (HCS-2/8) and osteosarcoma (Saos-2) cells in the serum of patients with osteoarthritis of the temporomandibular joint.
To find specific humoral antibodies in sera from patients with temporomandibular joint (TMJ) osteoarthritis (OA), an immortal human chondrocyte (HCS-2/8) and osteoblast (Saos-2) cell line derived from a chondrosarcoma and an osteosarcoma, respectively, were used as source proteins of human antigens. Patients with chronically painful TMJ OA (n = 18) but no other joints symptoms were selected from a consecutive series of patients with temperomandibular disorders and sex-matched asymptomatic controls (n = 8) were also recruited. Cellular proteins of the HCS-2/8 and Saos-2 cells were subjected to Western blotting with the OA and control sera as probes. Band-recognition frequency and the peak optical density of the band were compared between groups by chi2 and t-tests. OA sera recognized various bands for the chondrocytes, and one of these (47-kDa) was specific for the OA sera. In two OA patients whose treatment outcome was less favorable, the reactivity against the 47-kDa protein was relatively high. In addition, the OA sera clearly cross-reacted with recombinant HSP47. Based on these findings, an autoimmune reaction against chondrocytes could be one of the exaggerating and/or perpetuating mechanisms in the pathophysiology of osteoarthritic TMJs, and the humoral antibody titre against the HSP47-like protein derived from the chondrocytes could be one of the possible markers for the prognosis of the joint pathology.
Kuboki
T
T
Department of Fixed Prosthodontics, Japan. kuboki@dent.okayama-u.ac.jp
Hattori
T
T
Mizushima
T
T
Kanyama
M
M
Fujisawa
T
T
Yamashita
A
A
Takigawa
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood*; D001323:Autoantibodies / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D019902:Chondrocytes / Q000276:immunology; D002813:Chondrosarcoma / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood / Q000276:immunology*; D010006:Osteoblasts / Q000276:immunology; D012516:Osteosarcoma / Q000276:immunology*; D011379:Prognosis; D011994:Recombinant Proteins; D013705:Temporomandibular Joint Disorders / Q000097:blood / Q000276:immunology*; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D015415:Biomarkers; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; C494660:SERPINH1 protein, human