Synergistic effects of laminin and thyroid hormones on neuron polarity in culture.
This study is aimed to elucidate whether triiodothyronine (T3) and laminin have additive effects in regulating neural differentiation. We focused our attention on the expression of synapsin I, the 68 kDa component of the neurofilament triplet (NF-68), growth associated protein (GAP)-43 and microtubule associated protein (MAP)-2 as markers of synapses, cytoskeleton, axons and the somatodendritic domain, respectively. The addition of T3 to the medium of differentiating rat cortical neurons cultured on laminin did not have any effect on the concentration of these proteins, but was critical for their subcellular localization, suggesting a synergistic role of thyroid hormones and laminin in the establishment of neural polarity.
Savettieri
G
G
Istituto di Neuropsichiatria, Policlinico Universitario Paolo Giaccone, Palermo, Italy.
Licata
L
L
Catania
C
C
Raneri
R
R
Di Liegro
I
I
Cestelli
A
A
D000818:Animals; D016764:Cell Polarity / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology; D005333:Fetus; D019922:GAP-43 Protein / Q000032:analysis; D007797:Laminin / Q000494:pharmacology*; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D016900:Neurofilament Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016704:Synapsins / Q000032:analysis; D014284:Triiodothyronine / Q000494:pharmacology*
D019922:GAP-43 Protein; D007797:Laminin; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D016704:Synapsins; C066858:neurofilament protein NF 68; D014284:Triiodothyronine