[Inhibitory effect of IGF-II antisense RNA on malignant phenotype of hepatocellular carcinoma].
OBJECTIVE
Inhibitory effect of insulin like growth factor II (IGF-II) antisense RNA on malignant phenotype of hepatic cancer cells was studied.


METHODS
A 0.1 kb cDNA of human IGF-II was reversely inserted into a eukaryotic expression vector of pcDNA3 and an IGF-II antisense RNA expression vector of pIGF-II As was obtained. The pIGF-II As was then introduced into human hepatic cancer cell line SMMC-7721. The effect of IGF-II antisense RNA which was expressed by pIGF-II As on SMMC-7721 cell cycles progression was measured with FCM.


RESULTS
The coloning formation in anchorage-independent assay of SMMC-7721 cells with pIGF-II As was significantly decreased by comparison with control groups of SMMC-7721 cells and SMMC-7721 cells transfected with pcDNA3 vector alone. FCM analysis showed that the S phase of cell cycles for SMMC-7721 cells with pIGF-II As was increased, but there were no obvious changes in the control groups of SMMC-7721 cells and SMMC-7721 cells with pcDNA3.


CONCLUSIONS
The pIGF-II As of IGF-II antisense RNA acts as an inhibitor on carcinogenesis of the SMMC-7721 cells in vitro.
Yang
D
D
Department of Gastroenterology, Zhujiang Hospital, First Military Medical University, Guangzhou.
Zhang
M
M
Du
J
J
D000818:Animals; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000037:antagonists & inhibitors* / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D010641:Phenotype; D016372:RNA, Antisense / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016372:RNA, Antisense; D007335:Insulin-Like Growth Factor II