Electrochemotherapy against colorectal carcinoma: comparison of in vitro cytotoxicity of 5-fluorouracil, cisplatin and bleomycin.
Effectiveness of electrochemotherapy against colorectal carcinoma (CRC) was investigated in vitro using murine CRC cell lines. Electropermeabilization did not increase the sensitivity of CRC cells to 5-fluorouracil or cisplatin. Conversely, electropermeabilization markedly increased the sensitivity of CRC cells to bleomycin (BLM), resulting in 1,000-fold higher susceptibility. Subsequent analyses revealed that electropermeabilization significantly increased intracellular BLM levels of CRC cells. These results suggest that electrochemotherapy with BLM is a promising modality for the treatment of CRC, and that electrochemotherapy can be translated from the treatment of superficial tumors to the treatment of internal tumors including CRC.
Kuriyama
S
S
Third Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8522, Japan.
Matsumoto
M
M
Mitoro
A
A
Tsujinoue
H
H
Toyokawa
Y
Y
Nakatani
T
T
Yamazaki
M
M
Okamoto
S
S
Fukui
H
H
D000230:Adenocarcinoma / Q000139:chemically induced / Q000188:drug therapy* / Q000453:epidemiology / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D001761:Bleomycin / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D002463:Cell Membrane Permeability; D002945:Cisplatin / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000453:epidemiology / Q000473:pathology; D016895:Culture Media, Serum-Free; D004127:Dimethylhydrazines; D004354:Drug Screening Assays, Antitumor; D018274:Electroporation; D005472:Fluorouracil / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D016895:Culture Media, Serum-Free; D004127:Dimethylhydrazines; D001761:Bleomycin; D002945:Cisplatin; D005472:Fluorouracil