Platelet-activating factor and eicosanoids are mediators of local and systemic changes induced by immune-complexes in mice.
A passive Arthus reaction (AR) induced in the peritoneal cavity of mice was followed by increased local vascular permeability and haemoconcentration. The intensity of the increased vasopermeability was higher in BALB/c compared with C3H/HePas mice despite the latter being 10 times more sensitive to platelet-activating factor (PAF). C3H/HePas mice however, exhibited higher levels of haemoconcentration and shock-like symptoms. Both events were inhibited by the PAF antagonist, WEB 2170. Indomethacin reduced both pathological events whereas L663,536, that inhibits leukotrienes synthesis reduced haemoconcentration but only in BALB/c mice. PAF was released into the peritoneal cavity, peak release being at 10 min after induction of AR. Prostaglandin E2 (PGE2), thromboxane B2 (TXB2), leukotriene B4 (LTB4), and leukotriene C4/D4 (LTC4/D4) were also released at this time. Similar levels of PAF and eicosanoids were found in BALB/c and C3H/HePas mice except for LTB4, which was higher in C3H/HePas. It is concluded that PAF and eicosanoids are mediators of local and systemic changes induced by immune complexes in the peritoneal cavity of mice.
Steil
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Departamento de Imunologia, Universidade de São Paulo, Brazil.
Teixeira
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Jancar
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D000818:Animals; D000936:Antigen-Antibody Complex / Q000502:physiology*; D001183:Arthus Reaction / Q000503:physiopathology*; D002199:Capillary Permeability; D015777:Eicosanoids / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity; D010972:Platelet Activating Factor / Q000378:metabolism / Q000502:physiology*; D013045:Species Specificity
D000936:Antigen-Antibody Complex; D015777:Eicosanoids; D010972:Platelet Activating Factor