Postnatal development of action potential-induced dendritic calcium entry in neocortical layer II/III pyramidal cells.
By whole-cell patch-clamp recording and calcium imaging with fura-2, we investigated postnatal development of intracellular calcium dynamics in apical dendrites of layer II/III pyramidal cells in the rat visual cortex. Dendritic calcium increases, induced by single action potentials, occurred only slightly on postnatal days 8-10 (P8-10), and then underwent a gradual enhancement during the second postnatal week to become 2-3-fold larger on P16-18 than on P8-10. The results suggest that the developmental growth of calcium dynamics may play a critical role for functional development of neocortical neurons.
Isomura
Y
Y
Department of Integrative Brain Science, Graduate School of Medicine, Kyoto University, 606-8501, Kyoto, Japan.
Yamamoto
K
K
Hashimoto
K
K
Kato
N
N
D000200:Action Potentials / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D066298:In Vitro Techniques; D019579:Neocortex / Q000254:growth & development / Q000502:physiology*; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D002118:Calcium