title: Alpha1,3-fucosyltransferase IX (Fuc-TIX) is very highly conserved between human and mouse; molecular cloning, characterization and tissue distribution of human Fuc-TIX.
abstract: The amino acid sequence of Fuc-TIX is very highly conserved between mouse and human. The number of non-synonymous nucleotide substitutions of the Fuc-TIX gene between human and mouse was strikingly low, and almost equivalent to that of the alpha-actin gene. This indicates that Fuc-TIX is under a strong selective pressure of preservation during evolution. The human Fuc-TIX (hFuc-TIX) showed a unique characteristics, i.e. hFuc-TIX was not activated by Mn2+ and Co2+, whereas hFuc-TIV and hFuc-TVI were activated by the cations. The hFuc-TIX transcripts were abundantly expressed in brain and stomach, and interestingly were detected in spleen and peripheral blood leukocytes.
authors.affiliation: Division of Cell Biology, Institute of Life Science, Soka University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: T
authors.initials: T
authors.lastname: Kudo
authors.forename: H
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ikehara
authors.forename: S
authors.initials: S
authors.lastname: Nishihara
authors.forename: S
authors.initials: S
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Shiina
authors.forename: H
authors.initials: H
authors.lastname: Inoko
authors.forename: N
authors.initials: N
authors.lastname: Saitou
authors.forename: H
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D005647:Fucosyltransferases / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D015723:Gene Library; D014644:Genetic Variation; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase