title: gamma delta+ T cells regulate major histocompatibility complex class II(IA and IE)-dependent susceptibility to coxsackievirus B3-induced autoimmune myocarditis.
abstract: Coxsackievirus B3 (CVB3) infection induces myocardial inflammation and myocyte necrosis in some, but not all, strains of mice. C57BL/6 mice, which inherently lack major histocompatibility complex (MHC) class II IE antigen, develop minimal cardiac lesions despite high levels of virus in the heart. The present experiments evaluate the relative roles of class II IA and IE expression on myocarditis susceptibility in four transgenic C57BL/6 mouse strains differing in MHC class II antigen expression. Animals lacking MHC class II IE antigen (C57BL/6 [IA+ IE-] and ABo [IA- IE-]) developed minimal cardiac lesions subsequent to infection despite high concentrations of virus in the heart. In contrast, strains expressing IE (ABo Ealpha [IA- IE+] and Bl.Tg.Ealpha [IA+ IE+]) had substantial cardiac injury. Myocarditis susceptibility correlated to a Th1 (gamma interferon-positive) cell response in the spleen, while disease resistance correlated to a preferential Th2 (interleukin-4-positive) phenotype. Vgamma/Vdelta analysis indicates that distinct subpopulations of gamma delta+ T cells are activated after CVB3 infection of C57BL/6 and Bl.Tg.Ealpha mice. Depletion of gamma delta+ T cells abrogated myocarditis susceptibility in IE+ animals and resulted in a Th1-->Th2 phenotype shift. These studies indicate that the MHC class II antigen haplotype controls myocarditis susceptibility, that this control is most likely mediated through the type of gamma delta T cells activated during CVB3 infection, and finally that different subpopulations of gamma delta+ T cells may either promote or inhibit Th1 cell responses.
authors.affiliation: Department of Pathology, Department of Medicine, University of Vermont College of Medicine, Burlington, Vermont 05405, USA. shuber@salus.med.uvm.edu
authors.forename: S A
authors.initials: SA
authors.lastname: Huber
authors.forename: J E
authors.initials: JE
authors.lastname: Stone
authors.forename: D H
authors.initials: DH
authors.lastname: Wagner
authors.forename: J
authors.initials: J
authors.lastname: Kupperman
authors.forename: L
authors.initials: L
authors.lastname: Pfeiffer
authors.forename: C
authors.initials: C
authors.lastname: David
authors.forename: R L
authors.initials: RL
authors.lastname: O'Brien
authors.forename: G S
authors.initials: GS
authors.lastname: Davis
authors.forename: M K
authors.initials: MK
authors.lastname: Newell
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology; D003384:Coxsackievirus Infections / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000276:immunology; D029822:Enterovirus B, Human; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009205:Myocarditis / Q000276:immunology* / Q000473:pathology / Q000821:virology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D016692:Receptors, Antigen, T-Cell, gamma-delta