title: Analysis of human IgE epitope of Der f 2 with anti-Der f 2 mouse monoclonal antibodies.
abstract: Der f 2 is one of the major mite allergens recognized by human IgE antibodies of allergic patients. Using five anti-Der f 2 mouse monoclonal antibodies, human IgE epitopes of Der f 2 were analyzed. Among them, two monoclonal antibodies 15E11 and 13A4 inhibited the binding between Der f 2 and human IgE antibodies. To determine major IgE epitopes of Der f 2, epitopes for the monoclonal IgG antibodies were analyzed using 43 single site Der f 2 mutants constructed by site-directed mutagenesis. Binding ability of 13A4 and 15E11 was decreased by the amino acid replacement around the C-terminus, and around 73rd, respectively. These results suggest that the C-terminal portion and the central portion around 73rd of Der f 2 were recognized by human IgE antibodies as major epitopes. The location of the putative IgE epitopes on 3-D structure of Der f 2 is also discussed.
authors.affiliation: Bioscience Research and Development Laboratory, Asahi Breweries Ltd, Ibaraki, Japan. chiharu.nishiyama@asahibeer.co.jp
authors.forename: C
authors.initials: C
authors.lastname: Nishiyama
authors.forename: H
authors.initials: H
authors.lastname: Hatanaka
authors.forename: S
authors.initials: S
authors.lastname: Ichikawa
authors.forename: M
authors.initials: M
authors.lastname: Fukada
authors.forename: M
authors.initials: M
authors.lastname: Akagawa-Chihara
authors.forename: T
authors.initials: T
authors.lastname: Yuuki
authors.forename: T
authors.initials: T
authors.lastname: Yokota
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Okumura
mesh_terms: D000485:Allergens / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D039741:Antigens, Dermatophagoides; D018604:Epitope Mapping; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008925:Mites; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D000941:Antigens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D016056:Immunodominant Epitopes; D007073:Immunoglobulin E