Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization.
A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.
Bach
H
H
Institute for Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany. bach@gsf.de
Errampalli
D
D
Leung
K T
KT
Lee
H
H
Hartmann
A
A
Trevors
J T
JT
Munch
J C
JC
D001409:Bacillus cereus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005798:Genes, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods*; D006109:Poaceae; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology
D017931:DNA Primers; D015342:DNA Probes; D008666:Metalloendopeptidases