title: 8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate reduced [Ca2+]i elevation induced by histamine, serotonin, and glutamate in cultured calf basilar artery smooth muscle cells.
abstract: OBJECTIVE
  To study the effects of 8-(N,N-diethylamino)-n-octyl-3,4,5- trimethoxybenzoate (TMB-8) on intracellular free calcium ([Ca2+]i) in cultured calf basilar artery smooth muscle cells.
  
  
  METHODS
  [Ca2+]i was examined by a system of measurement of AR-CM-MIC, using Fura 2-AM as a fluorescent indicator.
  
  
  RESULTS
  In the presence of extracellular Ca2+ 1.3 mmol.L-1, histamine (His), serotonin (5-HT), and sodium glutamate (Glu) markedly increased the [Ca2+]i which was attenuated by TMB-8. In Ca2+ free Hanks' solution containing egtazic acid 0.1 mmol.L-1, TMB-8 not only reduced the resting [Ca2+]i, but also inhibited the elevation of [Ca2+]i evoked by His and 5-HT.
  
  
  CONCLUSIONS
  TMB-8 reduced the resting [Ca2+]i and attenuated His-, 5-HT-, and Glu-induced increases of [Ca2+]i in basilar artery smooth muscle cells.
authors.affiliation: Department of Pharmacology, Nanjing Medical University, China. qj73@hotmail.com
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhang
authors.forename: T P
authors.initials: TP
authors.lastname: Liu
authors.forename: J G
authors.initials: JG
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001488:Basilar Artery; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D012702:Serotonin Antagonists / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D006633:Histamine Antagonists; D012702:Serotonin Antagonists; D018698:Glutamic Acid; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D002118:Calcium