Dendritic cells and MPIF-1: chemotactic activity and inhibition of endogenous chemokine production by IFN-gamma and CD40 ligation.
We have examined the biological activity of the CC chemokine myeloid progenitor inhibitory factor 1 (MPIF-1) on human dendritic cells. MPIF-1 has chemotactic activity on dendritic cells derived from either peripheral blood monocytes or cord blood CD34+ progenitors. However, chemokine treatment did not induce further cell activation or maturation. In addition, MPIF-1 is constitutively released by monocyte-derived dendritic cells but not macrophages or monocytes (resting or stimulated). The proinflammatory stimuli lipopolysaccharide and tumor necrosis factor alpha, which induced the release of monocyte chemotactic protein-1, macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and interleukin-8, did not affect MPIF-1 release. In contrast, CD40 ligation and interferon-gamma treatment, while stimulating the production of the other chemokines, caused a pronounced reduction of MPIF-1 transcript and protein release. Thus, in dendritic cells the regulation of the production and release of MPIF-1 is distinct in comparison to other CC and CXC chemokines.
Nardelli
B
B
Human Genome Sciences, Inc., Rockville, Maryland 20850, USA. bernardetta_nardelli@hgsi.com
Morahan
D K
DK
Bong
G W
GW
Semenuk
M A
MA
Kreider
B L
BL
Garotta
G
G
D000970:Antineoplastic Agents / Q000494:pharmacology; D019742:Chemokines, CC / Q000502:physiology*; D003713:Dendritic Cells / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology; D011506:Proteins / Q000378:metabolism / Q000494:pharmacology; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology; D048008:TNF Receptor-Associated Factor 3; D016335:Zinc Fingers / Q000502:physiology
D000970:Antineoplastic Agents; C105504:CCL23 protein, human; D019742:Chemokines, CC; D011506:Proteins; D018124:Receptors, Tumor Necrosis Factor; D048008:TNF Receptor-Associated Factor 3; D007371:Interferon-gamma