A role for fasciclin II in the guidance of neuronal migration.
The insect cell adhesion receptor fasciclin II is expressed by specific subsets of neural and non-neural cells during embryogenesis and has been shown to control growth cone motility and axonal fasciculation. Here we demonstrate a role for fasciclin II in the guidance of migratory neurons. In the developing enteric nervous system of the moth Manduca sexta, an identified set of neurons (the EP cells) undergoes a stereotyped sequence of migration along the visceral muscle bands of the midgut prior to their differentiation. Probes specific for Manduca fasciclin II show that while the EP cells express fasciclin II throughout embryogenesis, their muscle band pathways express fasciclin II only during the migratory period. Manipulations of fasciclin II in embryonic culture using blocking antibodies, recombinant fasciclin II fragments, and enzymatic removal of glycosyl phosphatidylinositol-linked fasciclin II produced concentration-dependent reductions in the extent of EP cell migration. These results support a novel role for fasciclin II, indicating that this homophilic adhesion molecule is required for the promotion or guidance of neuronal migration.
Wright
J W
JW
Department of Cell and Developmental Biology L215, Oregon Health Sciences University, Portland, OR 97201, USA.
Snyder
M A
MA
Schwinof
K M
KM
Combes
S
S
Copenhaver
P F
PF
D000595:Amino Acid Sequence; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics* / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology; D017615:Enteric Nervous System / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D018402:Manduca / Q000196:embryology*; D008969:Molecular Sequence Data; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D015816:Cell Adhesion Molecules, Neuronal; D017261:Glycosylphosphatidylinositols; D020033:Protein Isoforms; C051642:fasciclin II