title: Establishment and characterization of normal and initiated hamster tracheal epithelial cell system.
abstract: The development and characterization of normal hamster tracheal epithelial (HTE) cell system and its initiated subline is described in the present study. Normal HTE cells grew in a monolayer, had a stable diploid karyotype, were anchorage dependent and non-tumorigenic. The presence of desmosomal attachments and keratin filaments confirmed the epithelial nature of these cells. An initiated subline DTC8 was isolated after treatment of HTE cells with a suboptimal dose of 9,10-dimethyl-1,2-benz[a]anthracene (DMBA). These DTC8 cells grew in a monolayer, had a higher growth rate and saturation density, were weakly anchorage independent and non-tumorigenic. Treatment of DTC8 cells with 100 ng 12-O-tetradecanoyl phorbol-13-acetate (TPA), resulted in transformation of these cells which then showed anchorage independent growth on semisolid agar and formed tumours in 85% animals. As DTC8 cells showed heterogeneity in chromosome number, they were further cloned by the limiting dilution method using gamma-irradiated hamster embryonic fibroblasts as a feeder layer. The clone H7I, isolated among these clones had all the properties of initiated cells.
authors.affiliation: Cell Biology Division, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai, India.
authors.forename: P D
authors.initials: PD
authors.lastname: Potdar
authors.forename: A N
authors.initials: AN
authors.lastname: Bhisey
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002875:Chromosomes; D003001:Cloning, Molecular; D006224:Cricetinae; D003896:Desmosomes / Q000648:ultrastructure; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D007621:Karyotyping; D007633:Keratins / Q000032:analysis; D008854:Microscopy, Electron; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014132:Trachea / Q000166:cytology*
chemical_list: D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene; D007633:Keratins; D013755:Tetradecanoylphorbol Acetate