title: Investigation of the local structure and dynamics of the H subunit of the mitochondrial glycine decarboxylase using heteronuclear NMR spectroscopy.
abstract: The lipoate-dependent H protein plays a pivotal role in the catalytic cycle of the glycine decarboxylase complex (GDC), undergoing reducing methylamination, methylene transfer, and oxidation. The local structure and backbone dynamics of the methylamine-loaded H (Hmet), oxidized H (Hox), and H apoprotein (Hapo) have been investigated in solution. Filtered NOESY experiments using a [13C]Hmet as well as comparison of the heteronuclear shifts between the Hox and Hmet proteins demonstrate that the methylamine group is located inside a cleft of the protein. Furthermore, this group appears to be locked in this configuration as indicated by the high value of the activation energy (37 kcal/mol) of the global unloading reaction and by its restricted mobility, deduced from 13C relaxation measurements. Comparisons of the 1H and 15N chemical shifts and 15N relaxation in the three forms suggest that part of the lipoyl-lysine arm interacts with the protein polypeptide in the Hox and Hmet. The major change induced by the loading of the methylamine group concerns the C-terminal helix whose mobility becomes completely restricted compared to those of the Hox and Hapo. This C-terminal helix exhibits different reorientational characteristics in the three forms, which can be explained in the Hapo by a model consisting of a twisting motion about an axis passing through the helix. Our results indicate that the model of a freely swinging arm proposed for other lipoate-containing proteins is not acceptable in solution for the GDC. The implication of this observation in terms of the mechanism of the interaction of the H protein with the T protein, its physiological partner during the catalytic cycle, is discussed.
authors.affiliation: Institut de Biologie Structurale Jean-Pierre Ebel (CEA-CNRS), Grenoble, France.
authors.forename: L
authors.initials: L
authors.lastname: Guilhaudis
authors.forename: J P
authors.initials: JP
authors.lastname: Simorre
authors.forename: M
authors.initials: M
authors.lastname: Blackledge
authors.forename: M
authors.initials: M
authors.lastname: Neuburger
authors.forename: J
authors.initials: J
authors.lastname: Bourguignon
authors.forename: R
authors.initials: R
authors.lastname: Douce
authors.forename: D
authors.initials: D
authors.lastname: Marion
authors.forename: P
authors.initials: P
authors.lastname: Gans
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D002247:Carbon Isotopes; D050959:Glycine Decarboxylase Complex; D050961:Glycine Decarboxylase Complex H-Protein; D050841:Glycine Dehydrogenase (Decarboxylating); D008744:Methylamines / Q000737:chemistry; D008928:Mitochondria / Q000201:enzymology*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D018532:Pisum sativum / Q000201:enzymology; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation; D011522:Protons; D013696:Temperature; D013816:Thermodynamics; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D050961:Glycine Decarboxylase Complex H-Protein; D008744:Methylamines; D010940:Plant Proteins; D011522:Protons; D008063:Thioctic Acid; C013091:lipoamide; C027451:methylamine; D000594:Amino Acid Oxidoreductases; D050959:Glycine Decarboxylase Complex; D050841:Glycine Dehydrogenase (Decarboxylating)