A filamentous phage of Vibrio parahaemolyticus O3:K6 isolated in Laos.
A filamentous phage, 'lvpf5,' of Vibrio parahaemolyticus O3:K6 strain LVP5 was isolated and characterized. The host range was not restricted to serotype O3:K6, but 7 of 99 V. parahaemolyticus strains with a variety of serotypes were susceptible to the phage. The phage was inactivated by heating at 80 C for 10 min and by treating with chloroform. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the phage exhibited a 3.8 kDa protein. The amino-terminal amino acid sequence of the coat protein was determined as AEGGAADPFEAIDLLGVATL. The phage genome consisted of a single-stranded DNA molecule. The activity of the phages was inhibited by anti-Na2 pili antibody.
Nakasone
N
N
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.
Ikema
M
M
Higa
N
N
Yamashiro
T
T
Iwanaga
M
M
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004196:Disease Outbreaks; D006801:Humans; D007812:Laos / Q000453:epidemiology; D008969:Molecular Sequence Data; D014735:Vibrio Infections / Q000453:epidemiology / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000821:virology*