title: The human melanocyte as a particular target for UVA radiation and an endpoint for photoprotection assessment.
abstract: The induction of DNA breaks by UVA (320-400 nm) in the nucleus of normal human melanocytes in culture was investigated using single cell gel electrophoresis, also called the comet assay. Endogenous pigment and/or melanin-related molecules were found to enhance DNA breakage: comets were more intense in melanocytes than in fibroblasts, in cells with high melanin content or after stimulation of melanogenesis by supplying tyrosine in the culture medium. After UVA doses where strong comets were observed, neither cytotoxicity nor stimulation of tyrosinase activity were detected. However, the accumulation of p53 protein suggested that cells reacted to genotoxic stress under these experimental conditions. The same approach was used to compare two sunscreens with identical sun protection factors but different UVA protection factors. The results presented in this paper suggest that human melanocytes may be used as a target cell to evidence broadspectrum photoprotection. Moreover, these data appear to be helpful in getting a better understanding of the role of sunlight in the initiating steps of melanocyte transformation.
authors.affiliation: L'Oreal Advanced Research, Life Sciences Research, Aulnay-Sous-Bois, France. lmarrot@loreal-recherche.com
authors.forename: L
authors.initials: L
authors.lastname: Marrot
authors.forename: J P
authors.initials: JP
authors.lastname: Belaidi
authors.forename: J R
authors.initials: JR
authors.lastname: Meunier
authors.forename: P
authors.initials: P
authors.lastname: Perez
authors.forename: C
authors.initials: C
authors.lastname: Agapakis-Causse
mesh_terms: D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D006801:Humans; D008543:Melanins / Q000378:metabolism; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D018462:Photobiology; D013473:Sunscreening Agents / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D008543:Melanins; D013473:Sunscreening Agents