title: Intestinal damage induced by zinc deficiency is associated with enhanced CuZn superoxide dismutase activity in rats: effect of dexamethasone or thyroxine treatment.
abstract: Zinc has a wide spectrum of biological activities and its deficiency has been related to various tissue dysfunctions and alterations of normal cell metabolism. Zinc also plays an important role in the antioxidant cellular defenses being a structural element of the non-mitochondrial form of the enzyme superoxide dismutase (CuZnSOD). We have already reported that Zn deficiency induces severe alterations in the rat intestine, that are reverted by treatment with dexamethasone (Dex) or thyroxine (T4). Here we report a paradoxical increase of CuZnSOD activity in rat intestine after 20 and 40 days of zinc deficiency. The increase of CuZnSOD activity is not due to an upregulation of gene expression because both Northern and Western blot analysis indicate that CuZnSOD mRNA and protein levels are not affected by zinc deficiency. A significant increase of lipid peroxidation was also observed in duodenum and jejunum associated with zinc deficiency. Treatment with either Dex or T4 to zinc-deficient rats protects against intestinal oxidative damage and results in SOD activity similar to control rats. Because glutathione peroxidase and catalase activities decreased in zinc deficiency, we speculate that the increase in SOD activity may be associated with an accumulation of hydrogen peroxide that may activate inflammatory molecules, further worsening tissue damage.
authors.affiliation: Istituto Nazionale della Nutrizione, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Virgili
authors.forename: R
authors.initials: R
authors.lastname: Canali
authors.forename: E
authors.initials: E
authors.lastname: Figus
authors.forename: F
authors.initials: F
authors.lastname: Vignolini
authors.forename: F
authors.initials: F
authors.lastname: Nobili
authors.forename: E
authors.initials: E
authors.lastname: Mengheri
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology* / Q000293:injuries*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology; D015032:Zinc / Q000172:deficiency*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D003907:Dexamethasone; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D015032:Zinc; D013974:Thyroxine