title: Relationship between lipid composition, frequency of ethanol-induced respiratory deficient mutants, and ethanol tolerance in Saccharomyces cerevisiae.
abstract: The frequency of ethanol-induced respiratory deficient mutants and lipid composition in two Saccharomyces cerevisiae strains showing different degrees of ethanol tolerance were investigated. The more ethanol-tolerant strain exhibited a lower frequency of ethanol-induced respiratory deficient mutants than the less ethanol-tolerant strain. In addition, the more ethanol-tolerant strain contained a higher ergosterol/phospholipid ratio, a higher proportion of phosphatidylcholine, a lower proportion of phosphatidylethanolamine, a higher incorporation of long-chain fatty acids in total phospholipids, and a slightly higher proportion of unsaturated fatty acids in total phospholipids than the less ethanol-tolerant strain. These results show a clear relationship between the lipid composition, the frequency of ethanol-induced respiratory deficient mutants, and the ethanol tolerance of S. cerevisiae. A possible explanation of this relationship is discussed.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Chi
authors.forename: N
authors.initials: N
authors.lastname: Arneborg
mesh_terms: D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D010101:Oxygen Consumption / Q000187:drug effects*; D010743:Phospholipids / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D000431:Ethanol