Quantitation of human haptoglobin: comparative ELISA studies using adsorption and capture methods.
Three ELISA methods for the quantitation of haptoglobin (Hp) in plasma and albumin are described: a polystyrene direct adsorption method and capture methods with antibody and hemoglobin. Hp aggregates generated by 60 degrees C heating showed as much as a hundred-fold higher response by polystyrene adsorption compared to the two capture methods, while unheated Hp showed comparable responses by the three methods.
Yee
E
E
Pharmaceutical Division, Bayer, Berkeley, CA 94701, USA.
Brown
J E
JE
D000327:Adsorption; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006242:Haptoglobins / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D012015:Reference Standards
D006242:Haptoglobins