Irradiation of amelanotic melanoma cells with 532 nm high peak power pulsed laser radiation in the presence of the photothermal sensitizer Cu(II)-hematoporphyrin: a new approach to cell photoinactivation.
Cu(II)-hematoporphyrin (CuHp) was efficiently accumulated by B78H1 amelanotic melanoma cells upon incubation with porphyrin concentrations up to 52 microM. When the cells incubated for 18 h with 13 microM CuHp were irradiated with 532 nm light from a Q-switched Nd: YAG laser operated in a pulsed mode (10 ns pulses, 10 Hz) a significant decrease in cell survival was observed. The cell photoinactivation was not the consequence of a photodynamic process, as CuHp gave no detectable triplet signal upon laser flash photolysis excitation and no decrease in cell survival was observed upon continuous wave irradiation. Thus, it is likely that CuHp sensitization takes place by photothermal pathways. The efficiency of the photoprocess was modulated by different parameters; thus, while varying the amount of added CuHp in the 3.25-26 microM range had little effect, pulse energies larger than 50 mJ and irradiation times of at least 120 s were necessary to induce a cell inactivation of about 50%. The porphyrin-cell incubation time prior to irradiation had a major influence on cell survival, suggesting that the nature of the CuHp microenvironment can control the efficiency of photothermal sensitization.
Soncin
M
M
Department of Biology, University of Padova, Italy. polo@civ.bio.unipd.it
Busetti
A
A
Fusi
F
F
Jori
G
G
Rodgers
M A
MA
D000818:Animals; D006415:Hematoporphyrins / Q000627:therapeutic use*; D053685:Laser Therapy; D008546:Melanoma, Experimental / Q000188:drug therapy*; D008665:Metalloporphyrins / Q000627:therapeutic use*; D051379:Mice; D018462:Photobiology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*
D006415:Hematoporphyrins; D008665:Metalloporphyrins; D017319:Photosensitizing Agents; C120350:copper(II)-hematoporphyrin