title: Effects of isoprothiolane on cell growth of cultured bovine mammary epithelial cells.
abstract: This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dose-dependent manner at the concentration of 0.05 to 5 microM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 microM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial cells. Moreover, the cytokines, IL-1alpha, IL-1beta, IL-6, and tumor necrosis factor (TNF)-alpha tended to inhibit the proliferation of mammary epithelial cells in a dose-dependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines.
authors.affiliation: Department of Veterinary Pathology, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: H
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Kiku
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: T J
authors.initials: TJ
authors.lastname: Rosol
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005260:Female; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013876:Thiophenes / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D005659:Fungicides, Industrial; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D013876:Thiophenes; D014409:Tumor Necrosis Factor-alpha; C023889:isoprothiolane; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases