Bone-bonding behavior of alumina bead composite.
Previously we developed an alumina bead composite (ABC) consisting of alumina bead powder (AL-P) and bisphenol-alpha-glycidyl methacrylate (Bis-GMA)-based resin and reported its excellent osteoconductivity in rat tibiae. In the present study, are evaluated histologically and mechanically the effect of alumina crystallinity on the osteoconductivity and bone-bonding strength of the composite. AL-P was manufactured by fusing crushed alpha-alumina powder and quenching it. The AL-P was composed mainly of amorphous and delta-crystal phases of alumina. Its average particle size was 3.5 microm, and it took a spherical form. Another composite (alpha ALC), filled with pure alpha-alumina powder (alpha AL-P), was used as a referential material. The proportion of powder added to each composite was 70% w/w. Mechanical testing of ABC and alpha ALC indicated that they would be strong enough for use under weight-bearing conditions. The affinity indices for ABC, determined using male Wistar rat tibiae, were significantly higher than those for alpha ALC (p < 0.0001) up to 8 weeks. Composite plates (15 x 10 x 2 mm) that had an uncured surface layer on one side were made in situ in a rectangular mold. One of the plates was implanted into the proximal metaphysis of the tibia of a male Japanese white rabbit, and the failure load was measured by a detaching test 10 weeks after implantation. The failure loads for ABC on its uncured surface [1.91+/-1.23 kgf (n = 8)] were significantly higher than those for alpha ALC on its uncured surface [0.35+/-0.33 kgf (n = 8); (p < 0.0001)], and they also were significantly higher than those for ABC on the other (cured surface) side (p < 0.0001). Histological examinations using rabbit tibiae revealed bone ingrowth into the composite only on the uncured surface of ABC. This study revealed that the amorphous phase of alumina and formation of an uncured surface layer are needed for the osteoconductive and bone-bonding ability of ABC. ABC shows promise as a basis for the development of a highly osteoconductive and mechanically strong biomaterial.
Shinzato
S
S
Department of Orthopeadic Surgery, Faculty of Medicine, Kyoto University, Shogoin, Japan.
Kobayashi
M
M
Choju
K
K
Kokubo
T
T
Nakamura
T
T
D000537:Aluminum Oxide; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate; D001826:Body Fluids; D001844:Bone Conduction; D001846:Bone Development; D001842:Bone and Bones / Q000033:anatomy & histology / Q000502:physiology*; D004548:Elasticity; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013718:Tensile Strength
D017438:Bisphenol A-Glycidyl Methacrylate; D000537:Aluminum Oxide