Tandem organization of medaka fish soluble guanylyl cyclase alpha1 and beta1 subunit genes. Implications for coordinated transcription of two subunit genes.
We determined the complete nucleotide sequences of the alpha1 subunit gene (OlGCS-alpha1) and the beta1 subunit gene (OlGCS-beta1) of medaka fish soluble guanylyl cyclase. In the genome, OlGCS-alpha1 and OlGCS-beta1 are organized in tandem. The two genes are only 986 base pairs apart and span approximately 34 kilobase pairs in the order of OlGCS-alpha1 and OlGCS-beta1. The nucleotide sequence of a large part of the 5'-upstream region of OlGCS-alpha1 is complimentarily conserved in that of OlGCS-beta1. To analyze the promoter activity of each gene, a fusion gene construct in which the 5'-upstream region was fused with the green fluorescent protein gene was injected into medaka fish 2-cell embryos. When the fusion gene containing the OlGCS-alpha1 upstream region was injected, green fluorescent protein fluorescence was detected in the embryonic brain. The 5'-upstream region of OlGCS-beta1 alone was insufficient for the reporter gene expression in the embryos. When the OlGCS-alpha1 upstream region was located upstream of the OlGCS-beta1-green fluorescence protein fusion gene, the reporter gene was expressed in the brain and trunk region of the embryos. These results suggest that the 5'-upstream region of OlGCS-alpha1 can affect the expression of OlGCS-beta1. It is therefore possible that the expression of OlGCS-alpha1 and OlGCS-beta1 is coordinated.
Mikami
T
T
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
Kusakabe
T
T
Suzuki
N
N
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006162:Guanylate Cyclase / Q000235:genetics*; D008164:Luminescent Proteins; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000235:genetics
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006162:Guanylate Cyclase