The subgenus-specific C-terminal region of protein IX is located on the surface of the adenovirus capsid.
We have investigated the antigenicity of the C- and N-terminal halves of pIX of human adenovirus types 2 and 3 (Ad2 and Ad3) as well as their orientations in virions. We found that only the C-terminal halves of Ad2 pIX and Ad3 pIX reacted in a subgenus-specific manner by enzyme-linked immunosorbent assay and immunoblot analysis. Based on immunoelectron microscopy experiments, pIX in viral capsids appears to be positioned such that the C-terminal part of pIX constitutes the surface domain whereas the N terminus of the protein makes up the internal domain in icosahedral Ad capsids.
Akalu
A
A
Institute of Medical Microbiology, Ernst-Moritz-Arndt-University, D-17487 Greifswald, Germany.
Liebermann
H
H
Bauer
U
U
Granzow
H
H
Seidel
W
W
D000260:Adenoviruses, Human / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D001665:Binding Sites; D002213:Capsid / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D036022:Capsid Proteins; D015870:Gene Expression; D006801:Humans; D009500:Neutralization Tests; D010957:Plasmids; D011817:Rabbits; D014771:Virion
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus