title: Construction and characterization of Escherichia coli disruptants defective in the yaeM gene.
abstract: Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kuzuyama
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Seto
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000096:biosynthesis* / Q000235:genetics*; D015139:Blotting, Southern; D002384:Catalysis; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics*; D009154:Mutation / Q000235:genetics / Q000502:physiology*; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D010428:Pentosephosphates / Q000378:metabolism; D010641:Phenotype; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: C109460:1-deoxylulose 5-phosphate; C114232:2-C-methylerythritol 4-phosphate; D004271:DNA, Fungal; D009097:Multienzyme Complexes; D010428:Pentosephosphates; D013403:Sugar Phosphates; D010088:Oxidoreductases; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; D019747:Aldose-Ketose Isomerases; D004896:Erythritol