title: Multi-enzymatic glucosylation using Eucalyptus UDP-glucosyltransferase coupled UDPglucose-fermentation by bakers' yeast.
abstract: The enzymatic synthesis of glucoside compounds using a membrane-associated UDP-glucosyltransferase fraction from Eucalyptus perriniana cultured cells as a water-insoluble catalyst (N. Nakajima, et. al., J. Ferment. Bioeng., 84 (5), pp. 455-460, 1997) has been effectively done by coupling UDPglucose-fermentation by bakers' yeast. For example, beta-thujaplicin (hinokitiol) and p-aminobenzoic acid were converted respectively to their corresponding beta-D-monoglucosides with the conversion rate of around 24-26% by the multi-enzymatic system with UDPglucose as a glucose donor, which is produced by yeast cells from glucose and 5'-UMP. Addition of either cellobiose, a substrate of beta-glucosidase, or DL-1,2-anhydro-myo-inositol, an inhibitor for the enzyme in the reaction mixture, could increased the yield of these beta-D-monoglucosides. This new enzymatic system could also be used for the synthesis of flavonoid glucosides such as isoquercitrin (quercetin 3-O-beta-D-glucoside).
authors.affiliation: Department of Nutritional Science, Okayama Prefectural University, Japan. nakajima@fhw.oka-pu.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.forename: H
authors.initials: H
authors.lastname: Hamada
authors.forename: S
authors.initials: S
authors.lastname: Yamane
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Furuya
mesh_terms: D005052:Eucalyptus / Q000201:enzymology*; D005285:Fermentation; D005964:Glucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D010946:Plants, Medicinal; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*
chemical_list: D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose