Prognostic significance of DNA ploidy pattern in osteosarcomas in association with chemotherapy.
In this study, we analysed the DNA ploidy of osteosarcomas at biopsy and attempted to clarify the relationship between DNA ploidy pattern and prognosis. Thirty patients with non-metastatic osteosarcoma of an extremity were studied. All underwent intensive chemotherapy with doxorubicin, cisplatin and methotrexate, in addition to wide tumor resection. DNA ploidy was detected by DNA cytofluorometry, using isolated and smeared cells of biopsied tumor tissue. Twelve tumors showed a diploid ploidy pattern and 18 showed a non-diploid pattern such as aneuploidy (15 tumors) and euploid-polyploidy (3 tumors). The event-free survival rate at 9 years was 63.5% in non-diploid osteosarcoma patients and 13.3% in diploid osteosarcoma patients. There was a statistically significant difference between the two groups (P = 0.0278). These results lead us to conclude that a non-diploid osteosarcoma may be more sensitive to chemotherapy than a diploid tumor.
Kusuzaki
K
K
Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, Hirokoji, Japan.
Takeshita
H
H
Murata
H
H
Hirata
M
M
Hashiguchi
S
S
Ashihara
T
T
Hirasawa
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001859:Bone Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000601:surgery; D002648:Child; D004273:DNA, Neoplasm / Q000187:drug effects; D018572:Disease-Free Survival; D005260:Female; D016195:G2 Phase / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000188:drug therapy / Q000235:genetics* / Q000601:surgery; D011003:Ploidies; D011379:Prognosis; D016196:S Phase / Q000235:genetics
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm