An expressional system of human cytochrome P-450 CYP1A1 gene transcription.
OBJECTIVE
To explore an expressional system of human cytochrome P-450 CYP1A1 (CYP1A1) gene transcription.


METHODS
The plasmid pMC 6.3 K containing human CYP1A1 promoter was transiently transfected into Hep G2 cells. The expression of chloramphenical acetyltransferase (CAT) reporter gene was detected by ELISA.


RESULTS
Both the CAT expression and CYP1A1 activity increased with the concentrations of beta-naphthoflavone from 2.5 to 10 mumol.L-1. At 10 mumol.L-1 of beta-naphthoflavone, the levels of CAT and CYP1A1 were 94-fold and 2.8-fold those of the corresponding control, respectively. Using this method, the study of 8 glucosinolates with various side chains on the induction of CYP1A1 gene transcription showed that none of the parent glucosinolates increased CAT expression, whereas the breakdown products of indol-3-yl-methyl glucosinolate (glucobrassicin), rather than indole-3-carbinol, increased the CAT expression.


CONCLUSIONS
The CYP1A1 gene transcriptional system was more reliable and sensitive.
Wang
H
H
Department of Food Molecular Biochemistry, Institute of Food Research, Norwich, UK. clbwhcbd@public.hb.cn
Williamson
G
G
Peng
R X
RX
D001937:Brassica / Q000737:chemistry; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter; D005961:Glucosinolates / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007211:Indoles; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured; D019324:beta-Naphthoflavone / Q000494:pharmacology
D005961:Glucosinolates; D007211:Indoles; D019324:beta-Naphthoflavone; C048308:glucobrassicin; D019363:Cytochrome P-450 CYP1A1; D015500:Chloramphenicol O-Acetyltransferase