Detection of IgG antibody to bovine leukaemia virus in urine and serum by two enzyme immunoassays.
Four hundred blood sera from a cattle production unit were tested for BLV-(Bovine Leukaemia Virus) antibody with IP (Institut Porquier) and SB (Svanova Biotech) ELISA kits. Seventy-seven cattle with BLV-antibody (19.25%) and 77 without the antibody were used. No significant difference was found between O.D. of sera of PL+ (Persistent Lymphocytosis Positive) and PL- (Negative) cattle. The mean O.D. of urine samples of 77 seropositive cattle was significantly higher than that of 77 seronegative cattle (P < 0.01). There were also differences between urine O.D.s of seropositive (PL+) and seropositive (PL-) groups of cattle with IP (P < 0.05) and SB (P < 0.01) kits. All the results revealed the presence of BLV-antibody in the urine of the cattle without any urinary dysfunction.
Carli
K T
KT
Department of Microbiology, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey. tayfun@uu20.bim.uludag.edu.tr
Sen
A
A
Batmaz
H
H
Kennerman
E
E
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000652:urine*; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood / Q000652:urine; D001909:Leukemia Virus, Bovine / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G