Anti-telomerase therapy suppressed glioma proliferation.
In order to determine if telomerase activity contributes to the growth of glioma cells, we constructed an anti-telomerase vector to suppress telomerase expression in glioma cells. The human telomerase (hTR)-antisense vector showed a significant suppression effect. However it did not appear to induce cell death as a stable population of cells survived more than two months following transfection. These results provide evidence that reagents aimed at inhibiting telomerase may represent a useful strategy for suppressing the growth of glioma cells. In addition to telomerase, another mechanism would also maintain telomere length, thus supporting continuous cell proliferation.
Yamaguchi
F
F
Department of Neurosurgery, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, Japan.
Morrison
R S
RS
Takahashi
H
H
Teramoto
A
A
D002455:Cell Division; D005822:Genetic Vectors; D005910:Glioma / Q000188:drug therapy* / Q000201:enzymology; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D016376:Oligonucleotides, Antisense; D019098:Telomerase