A convenient and efficient microsatellite-based assay for resolving parentages in dogs.
We present a practical and efficient parentage analysis test for dogs. The method makes use of ten polymorphic microsatellite markers combined in three multiplex-PCR reactions. All three multiplexes can be performed under the same cycling conditions and using a multiple-dye detection system the PCR-products can be pooled for electrophoresis. Amplifications in four different thermal cyclers produced easily interpretable results throughout demonstrating the repeatability of the assay. In order to evaluate the method's efficiency a total of 100 dogs from four different breeds were typed. Assuming one known parent, exclusion probabilities ranging from 99.34% to 99.93% were attained.
Koskinen
M T
MT
Department of Animal Science, University of Helsinki, Finland.
Bredbacka
P
P
D000818:Animals; D001947:Breeding; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D004285:Dogs / Q000235:genetics*; D004586:Electrophoresis / Q000662:veterinary; D005260:Female; D006197:Hair / Q000737:chemistry; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010290:Parents; D016133:Polymerase Chain Reaction / Q000662:veterinary
D017931:DNA Primers; D004247:DNA