title: Small bowel ischaemia-reperfusion increases plasma concentrations of oxidised proteins in rats.
abstract: OBJECTIVE
  To find out whether plasma concentrations of protein carbonyl (a specific marker of oxidative damage of proteins) are increased during intestinal ischaemia-reperfusion and whether they are correlated with von Willebrand's factor (vWF, a marker of endothelial injury) or myeloperoxidase (a marker of neutrophil activation).
  
  
  METHODS
  Randomised experimental study.
  
  
  METHODS
  University department of surgery, New Zealand.
  
  
  METHODS
  Thirty anaesthetised adult Wistar rats.
  
  
  METHODS
  The sham operated group (n = 10) had laparotomy and isolation of the superior mesenteric artery without clamping. The ischaemia-reperfusion group (IR, n = 10) had the superior mesenteric artery clamped for 1 hour and reperfusion for 15 minutes. The control group (n = 10) had direct puncture of the heart to sample blood.
  
  
  METHODS
  Plasma concentrations of protein carbonyl, vWF, and myeloperoxidase.
  
  
  RESULTS
  Plasma protein carbonyl concentrations were significantly higher in the IR group than in the sham group (p < 0.02, Mann-Whitney test, median (range) 0.187 (0.141-0.242) compared with 0.144 (0.121-0.185) nmol/mg) and in the control group (p < 0.01, Mann-Whitney test, median (range) 0.187 (0.141-0.242) compared with 0.136 (0.108-0.175) nmol/mg). There was a significant correlation between protein carbonyl and vWF concentrations (r = 0.54, F = 10.9, p < 0.003, linear regression) but not with those of myeloperoxidase.
  
  
  CONCLUSIONS
  Intestinal ischaemia-reperfusion caused an increase in the plasma protein carbonyl concentration, which is possibly produced by endothelial cells.
authors.affiliation: Department of Surgery, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
authors.forename: F M
authors.initials: FM
authors.lastname: Abu-Zidan
authors.forename: C C
authors.initials: CC
authors.lastname: Winterbourn
authors.forename: M J
authors.initials: MJ
authors.lastname: Bonham
authors.forename: M O
authors.initials: MO
authors.lastname: Simovic
authors.forename: H
authors.initials: H
authors.lastname: Buss
authors.forename: J A
authors.initials: JA
authors.lastname: Windsor
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress; D009195:Peroxidase / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood*; D013997:Time Factors; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D014841:von Willebrand Factor; D009195:Peroxidase