[Glycodeoxycholic acid changes the membrane fluidity and superoxides of lipids in hepatocytes].
OBJECTIVE
To explore the mechanisms of hepatocyte injury caused by glycodeoxycholic acid (GDCA) through studies on the roles of GDCA to membrane fluidity and superoxides of lipids of hepatocyte.


METHODS
GDCA was used to treat hepatocytes of rat cultured in vitro and changes in cell membrane fluidity and malondialdehyde(MDA) were observed.


RESULTS
In the presence of GDCA (final concentration of 250 mumol/L), the concentration of hepatocyte MDA of GDCA increased significantly (P < 0.001) and membrane fluidity decreased (P < 0.02) after culture of 1 or 4 hours. ALT was directly related to MDA and fluorescence polarization (P), r was 0.945 and 0.986, respectively.


CONCLUSIONS
GDCA can induce the increase of MDA and decrease of fluidity of hepatocyte membrane, and finally cause hepatocyte injury.
Huang
W
W
Department of Clinical Laboratory, Chongqing University of Medical Sciences.
Kang
G
G
Tu
Z
Z
D000818:Animals; D002478:Cells, Cultured; D006002:Glycodeoxycholic Acid / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D006002:Glycodeoxycholic Acid