Neurons induce GFAP gene promoter of cultured astrocytes from transgenic mice.
In order to investigate the influence of neuron-glia interaction on astrocyte differentiation, we used a transgenic mouse bearing part of the gene promoter of the astrocytic maturation marker GFAP linked to the beta-galactosidase (beta-gal) reporter gene. Addition of embryonic cerebral hemisphere (CH) neurons to transgenic CH astrocyte monolayers increased by 50-60% beta-gal positive cell number. Such event was dependent on the brain regional origin of the neurons and was followed by an arrest of astrocytes from the cell cycle and induction of glial differentiation. Time-course assays demonstrated that maximum effect was observed after 24 h of coculture. Addition of conditioned medium (CM) derived from CH neurons also increased beta-gal positive CH astrocytic cell number. However, such CM had no effect on midbrain and cerebellum astroglia. Together, these data suggest that neurons secrete brain region-specific soluble factors which induce GFAP gene promoter, as measured by beta-gal expression, thus suggesting that neuron-glia interaction might induce the astrocytic differentiation program.
Gomes
F C
FC
Departamento de Anatomia, Universidade Federal do Rio de Janeiro, Brazil.
Garcia-Abreu
J
J
Galou
M
M
Paulin
D
D
Moura Neto
V
V
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D004058:Diffusion; D015870:Gene Expression / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics*; D007763:Lac Operon / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein