Differential effects of iron load on basal and interferon-gamma plus lipopolysaccharide enhance anticryptococcal activity by the murine microglial cell line BV-2.
Here we evaluated the influence of intracellular iron levels on the constitutive and interferon (IFN)-gamma plus lipopolysaccharide (LPS) enhanced anticryptococcal activity by the murine microglial cell line BV-2. We demonstrated that iron loading via ferric nitrilotriacetate (FeNTA) resulted in a significant increase in the constitutive levels of anticryptococcal activity, while the enhancing effects by IFN-gamma plus LPS were prevented. Accordingly, a major increase was observed in the levels of thiobarbituric reactive substance (TBARS) produced upon iron loading under basal conditions, whereas IFN-gamma plus LPS treatment, that per se did not affect TBARS production, prevented by about 50% the enhancement otherwise occurring in response to iron loading. The potential involvement of multiple effector system and their relation to intracellular iron will be discussed.
Saleppico
S
S
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Boelaert
J R
JR
Omodeo Salè
F
F
Mazzolla
R
R
Morucci
P
P
Bistoni
F
F
Blasi
E
E
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology; D003453:Cryptococcosis / Q000276:immunology* / Q000378:metabolism / Q000628:therapy; D005290:Ferric Compounds / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D002273:Carcinogens; D005290:Ferric Compounds; D008070:Lipopolysaccharides; D017392:Thiobarbituric Acid Reactive Substances; D007371:Interferon-gamma; D007501:Iron; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate