Cell-density (cycle) dependent silencer of the rat insulin-like growth factor binding protein-2 (IGFBP-2) gene.
An element which has a negative effect on transcription has been identified in the 5'-flanking region of the rat insulin-like growth factor binding protein-2 (IGFBP-2) gene. This element was confirmed to be a silencer by truncation or by linking it to a heterologous promoter. The silencer activity disappeared in the growth-arrested BRL-3A cells at the G1/S border by high cell density where the IGFBP-2 production is highly elevated. This observation may represent a novel mechanism through which gene expression is controlled by modulation of a silencer. Taken together, these results suggest a regulatory link between cell growth and IGFBP-2 expression regulated by its silencer.
Kutoh
E
E
Molekulare Endokrinologie, Zentrum für Lehre und Forschung, Kantonsspital Basel, Switzerland. ekuto@janbe.jnj.com
Margot
J
J
Schwander
J
J
D000818:Animals; D002452:Cell Count / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics* / Q000502:physiology*; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D011401:Promoter Regions, Genetic; D051381:Rats; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D018971:Insulin-Like Growth Factor Binding Protein 2