Bone morphogenetic protein-2 increases the rate of callus formation after fracture of the rabbit tibia.
The effects of human recombinant bone morphogenetic protein-2 (rhBMP-2) on rabbit fractures healing under both stable and unstable mechanical conditions were investigated. rhBMP-2 was administered (1) on bioerodible particles, (2) in a collagen gel, and (3) by injection. rhBMP-2 on bioerodible particles has no effect as the particles prevent the migration of cells that produce the callus. The collagen gel is resorbed more rapidly; the development of the callus of mechanically unstable fractures is similar to controls at 14 days. When rhBMP-2 is injected, the callus of mechanically unstable fractures develops more rapidly so that cortical union occurs by 21 days, as compared with 28 days in control fractures. The effects on fractures healing under stable mechanical conditions are minimal. It is argued that mechanical factors influence the size of the callus of normally healing fractures and, although BMP-2 accelerates the rate of development of the callus and cortical union, it does not affect the amounts of bone and cartilage produced.
Bax
B E
BE
Department of Anatomy, St. George's Hospital Medical School, Tooting, London, SW17 ORE, UK.
Wozney
J M
JM
Ashhurst
D E
DE
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D002146:Bony Callus / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D002356:Cartilage / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D003094:Collagen; D004333:Drug Administration Routes; D004337:Drug Carriers; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013978:Tibial Fractures / Q000188:drug therapy*; D016212:Transforming Growth Factor beta; D016474:Weight-Bearing / Q000502:physiology
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen