title: [A clinical application of laser scanning cytometer--the significance in the cytology as an additional diagnostic technique].
abstract: Laser scanning cytometer (LSC) is a new machine similar to flow cytometer but with advantages for certain clinical and research applications. LSC is a microscope based and measures cells on a slide with the position of each cell on the slide. This new technique of LSC can be utilized on extremely small specimens and enables direct correlation of all of the measured fluorescent parameters with light microscopic cytologic morphology. To date, LSC has been successfully used to perform DNA content analysis of numerous specimen types and automated analysis of fluorescence in situ hybridization specimens. In this report, we describe characteristics of LSC comparison with flow cytometry and a clinical application of LSC focused on DNA content analysis in clinical specimens with pulmonary disorders. LSC provides a number of benefits that may make it more suitable for clinical laboratories than FCM.
authors.affiliation: Department of Pulmonary Diseases and Clinical Research Institute, Sapporo National Hospital.
authors.forename: H
authors.initials: H
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Tokuchi
authors.forename: M
authors.initials: M
authors.lastname: Kamachi
authors.forename: M
authors.initials: M
authors.lastname: Harada
authors.forename: S
authors.initials: S
authors.lastname: Matsubayashi
authors.forename: K
authors.initials: K
authors.lastname: Doda
authors.forename: K
authors.initials: K
authors.lastname: Yamashiro
mesh_terms: D001980:Bronchi / Q000166:cytology; D003581:Cytodiagnosis / Q000295:instrumentation*; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis; D010996:Pleural Effusion / Q000166:cytology
chemical_list: D004273:DNA, Neoplasm; D004247:DNA