Detection of cerebroside sulfotransferase mRNA in human gastric mucosa and adenocarcinoma.
Sulfatide is a major acidic glycolipid in human gastric mucosa, and its sulfation is catalyzed by cerebroside sulfotransferase (CST). To investigate the expression of the CST gene in human gastric cancer, a reverse transcription PCR method was developed with the use of endoscopic bioptic specimens. By this method, we examined the CST mRNA expression in 11 cases of gastric cancer, and in all the cases we detected various levels of the expression both in cancer tissues and in uninvolved adjacent tissues. The present assay method was suggested to be useful in the detection of CST mRNA from a limited amount of bioptic samples.
Kobayashi
T
T
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Honke
K
K
Tsunematsu
I
I
Kagaya
H
H
Nishikawa
S
S
Hokari
K
K
Kato
M
M
Takeda
H
H
Sugiyama
T
T
Higuchi
A
A
Asaka
M
M
D000230:Adenocarcinoma / Q000201:enzymology* / Q000382:microbiology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000382:microbiology; D015238:Sulfotransferases / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase