Pim kinase expression is induced by LTP stimulation and required for the consolidation of enduring LTP.
In animals and several cellular models of synaptic plasticity, long-lasting changes in synaptic strength are dependent on gene transcription and translation. Here we demonstrate that Pim-1, a serine/threonine kinase closely related to Pim-2 and Pim-3, is induced in hippocampus in response to stimuli that evoke long-term potentiation (LTP). Mice deficient for Pim-1 show normal synaptic transmission and short-term plasticity. However, they fail to consolidate enduring LTP even though Pim-2 and Pim-3 are constitutively expressed in the hippocampus and Pim-3 expression is similarly induced by synaptic activity. Thus, expression of Pim-1 is required for LTP. Its level of expression and, consequently, its capacity to phosphorylate target proteins in dendritic and nuclear compartments of stimulated neurons might be a determining factor for the establishment of long-lasting changes in synaptic strength.
Konietzko
U
U
Zentrum für Molekulare Neurobiologie (ZMNH), University of Hamburg, Martinistrasse 52, D-20246 Hamburg, Germany.
Kauselmann
G
G
Scafidi
J
J
Staubli
U
U
Mikkers
H
H
Berns
A
A
Schweizer
M
M
Waltereit
R
R
Kuhl
D
D
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D003001:Cloning, Molecular; D003712:Dendrites / Q000201:enzymology / Q000378:metabolism; D004594:Electrophysiology; D004790:Enzyme Induction; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051573:Proto-Oncogene Proteins c-pim-1; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics; D009435:Synaptic Transmission
C094167:Pim2 protein, mouse; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C496636:Pim1 protein, mouse; C496637:Pim1 protein, rat; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1