Unique 5'-end of a Na(+)-K(+)-2Cl- cotransporter-like mRNA expressed in rat skeletal muscle.
Functional evidence presented by others indicates that rat slow-twitch skeletal muscle lacks typical Na(+)-K(+)-2Cl- cotransporter activity, as determined by loop diuretic-sensitive potassium transport. This report presents a unique 5' mRNA sequence of a Na(+)-K(+)-2Cl- cotransporter-like molecule expressed in the rat soleus muscle and the deduced N-terminus of the protein. In addition to its unique 5' mRNA sequence, the coding region of the N-terminus is quite short compared with other known Na(+)-K(+)-2Cl- cotransporters. Nonetheless, the mRNA possesses conserved cotransporter-like membrane spanning domains, though one domains corresponding to a reported exon is divergent. Therefore, it appears that skeletal muscle does express a Na(+)-K(+)-2Cl- cotransporter-like mRNA that may code for a protein with atypical Na(+)-K(+)-2Cl- cotransporter properties.
Fu
L
L
Department of Physiology, University of Tennessee, Memphis 38163, USA.
Wong
J A
JA
Schneider
E G
EG
Thomason
D B
DB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D028021:Sodium-Potassium-Chloride Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D028021:Sodium-Potassium-Chloride Symporters