title: Skeletal muscle and small-conductance calcium-activated potassium channels.
abstract: Skeletal muscle becomes hyperexcitable following denervation and when cultured in the absence of nerve cells. In these circumstances, the bee venom peptide toxin apamin, a blocker of small-conductance calcium-activated potassium (SK) channels, dramatically reduces the hyperexcitability. In this report, we show that SK3 channels are expressed in denervated skeletal muscle and in L6 cells. Action potentials evoked from normal innervated rat skeletal muscle did not exhibit an afterhyperpolarization, indicating a lack of SK channel activity; very low levels of apamin binding sites, SK3 protein, or SK3 mRNA were present. However, denervation resulted in apamin-sensitive afterhyperpolarizations and increased apamin binding sites, SK3 protein, and SK3 mRNA. Cultured rat L6 myoblasts and differentiated L6 myotubes contained similar levels of SK3 mRNA, although apamin-sensitive SK currents and apamin binding sites were detected only following myotube differentiation. Therefore, different molecular mechanisms govern SK3 expression levels in denervated muscle compared with muscle cells differentiated in culture.
authors.affiliation: Department of Cell and Developmental Biology, Oregon Health Sciences University, Portland, USA.
authors.forename: D
authors.initials: D
authors.lastname: Pribnow
authors.forename: T
authors.initials: T
authors.lastname: Johnson-Pais
authors.forename: C T
authors.initials: CT
authors.lastname: Bond
authors.forename: J
authors.initials: J
authors.lastname: Keen
authors.forename: R A
authors.initials: RA
authors.lastname: Johnson
authors.forename: A
authors.initials: A
authors.lastname: Janowsky
authors.forename: C
authors.initials: C
authors.lastname: Silvia
authors.forename: M
authors.initials: M
authors.lastname: Thayer
authors.forename: J
authors.initials: J
authors.lastname: Maylie
authors.forename: J P
authors.initials: JP
authors.lastname: Adelman
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002874:Chromosome Mapping; D015894:Genome, Human; D006801:Humans; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000502:physiology*; D009431:Neural Conduction; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D013234:Stem Cells / Q000187:drug effects
chemical_list: D015221:Potassium Channels; D002118:Calcium