A chromosomal paracentric inversion associated with T-DNA integration in Arabidopsis.
T-DNA integration in the nuclear plant genome may lead to rearrangements of the plant target site. Here we present evidence for a chromosomal inversion of 26 cM bordered by two T-DNAs in direct orientation, which is linked to the mgoun2 mutation. The integration sites of the T-DNAs map at positions 80 and 106 of chromosome I and we show that each T-DNA is bordered by plant sequences from positions 80 and 106, respectively. Although the T-DNAs are physically distant, they are genetically closely linked. In addition, three markers located on the chromosome segment between the two T-DNA integration sites show no recombination with the mgo2 mutation. We show that the inversion cannot be a consequence of a recombination event between the two T-DNAs, but that the integration of the T-DNAs and the inversion were two temporally linked events. T-DNA integration mechanisms that could have led to this inversion are discussed.
Laufs
P
P
Institut National de la Recherche Agronomique, Laboratoire de Biologie Cellulaire, Versailles, France. patrick.laufs@bbsrc.ac.uk
Autran
D
D
Traas
J
J
D017360:Arabidopsis / Q000235:genetics*; D007446:Chromosome Inversion; D002875:Chromosomes; D004269:DNA, Bacterial / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006579:Heterozygote; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D005819:Genetic Markers; D010940:Plant Proteins; C036483:T-DNA