title: Chlamydia trachomatis nucleic acids can be found in the synovium of some asymptomatic subjects.
abstract: OBJECTIVE
  The recent identification of antigens or nucleic acids of infectious agents in the joints of patients with reactive arthritis has raised questions about whether chlamydial or other infectious agent nucleic acids are also present in normal joints. We had the opportunity to study synovium from 30 asymptomatic volunteer subjects by use of polymerase chain reaction (PCR) for attempted identification of Chlamydia and other infectious agents.
  
  
  METHODS
  All subjects had blind needle synovial biopsies with the Parker-Pearson needle. DNA was extracted and PCR performed using primers for Chlamydia trachomatis, Chlamydia pneumoniae, Borrelia burgdorferi, and pan bacterial 16S ribosomal RNA (rRNA).
  
  
  RESULTS
  Two subjects were identified with nucleic acid for the 16S rRNA gene of C trachomatis. All other PCR reactions were negative except for the pan bacterial 16S rRNA in the C trachomatis-positive subjects. Both subjects, although symptom free, had some evidence of synovial reaction.
  
  
  CONCLUSIONS
  C trachomatis appears to occasionally be disseminated to joints without producing overt disease.
authors.affiliation: Department of Veterans Affairs Medical Center, Philadelphia, Pennsylvania 19104, USA.
authors.forename: H R
authors.initials: HR
authors.lastname: Schumacher
authors.forename: T
authors.initials: T
authors.lastname: Arayssi
authors.forename: M
authors.initials: M
authors.lastname: Crane
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Gerard
authors.forename: A P
authors.initials: AP
authors.lastname: Hudson
authors.forename: J
authors.initials: J
authors.lastname: Klippel
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D007719:Knee Joint / Q000000981:diagnostic imaging; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011859:Radiography; D013583:Synovial Membrane / Q000382:microbiology* / Q000473:pathology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S