title: Formation of a chiral acetoinic compound from diacetyl by Escherichia coli expressing meso-2,3-butanediol dehydrogenase.
abstract: L-Acetoin (L-AC) was produced from diacetyl (DA) by Escherichia coli JM109/pBUD119 containing the meso-2,3-butanediol dehydrogenase (D-AC forming) gene. However, when the strain was cultured in the presence of isopropyl-beta-D-thiogalacto-pyranoside, the enzyme formed catalysed not only D-AC but also L-AC. L-AC was further reduced to L-2,3-butanediol (BD). The yield of L-AC or L-BD from DA (3 gl-1) was about 70% (w/w).
authors.affiliation: Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ui
authors.forename: A
authors.initials: A
authors.lastname: Mimura
authors.forename: M
authors.initials: M
authors.lastname: Ohkuma
authors.forename: T
authors.initials: T
authors.lastname: Kudo
mesh_terms: D000093:Acetoin / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D002072:Butylene Glycols / Q000378:metabolism; D003931:Diacetyl / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013237:Stereoisomerism
chemical_list: D002072:Butylene Glycols; D000093:Acetoin; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; D003931:Diacetyl