title: Negative correlation between cyclophilin mRNA level in leukocytes of renal allograft recipients and ciclosporin concentration in whole blood.
abstract: OBJECTIVE
  To study mRNA level of cyclophilin in the white blood cells (WBC) of the renal allograft recipients (RAR) and its correlation with ciclosporin concentration in whole blood.
  
  
  METHODS
  The cyclophilin mRNA levels and beta-actin as controls in the WBC of 47 RAR were measured by quantitative reverse transcription polymerase chain reaction. The blood ciclosporin assay utilized monoclonal antibody fluorescence polarization immunoassay.
  
  
  RESULTS
  With the increase of ciclosporin concentration in whole blood (from 62 micrograms.L-1 to 678 micrograms.L-1), relative cyclophilin mRNA level in the WBC of RAR decreased nonlinearly (from 1.1 to 0.03, r = 0.8195).
  
  
  CONCLUSIONS
  There was a negative correlation between the mRNA level of cyclophilin in the WBC of RAR and the ciclosporin concentration in whole blood.
authors.affiliation: Molecular Biology Laboratory, Jinling Hospital, Nanjing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: F Q
authors.initials: FQ
authors.lastname: Li
authors.forename: J G
authors.initials: JG
authors.lastname: Wu
authors.forename: X M
authors.initials: XM
authors.lastname: Chu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: X D
authors.initials: XD
authors.lastname: Yao
mesh_terms: D000293:Adolescent; D000328:Adult; D016572:Cyclosporine / Q000097:blood*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D016030:Kidney Transplantation; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D016572:Cyclosporine; D019696:Peptidylprolyl Isomerase