Phenotypic selection: a successful strategy to fix major genes of hypertension.
Hypertension is dominantly inherited in cross hybrids between hypertensive SHR/Mol and normotensive BB/OK rats. We used these cross hybrids for repeated backcrossing of selected hypertensive animals onto normotensive BB/OK rats to fix high blood pressure and to generate a hypertensive and diabetic BB/OK rat subline. After 8 backcrosses, the backcross parents were genetically analysed with the aid of 259 microsatellite markers to identify SHR genes causing blood pressure of 177 +/- 10 mmHg in this BB/OK rat subline. Loci on chromosomes 1, 14 and 18 showed longest heterozygosity. These loci might contain major genes of the SHR rat causing hypertension in this BB/OK rat subline. This classical strategy seems to be most suitable to fix major genes of hypertension in particular and complex traits in general and therefore to generate new animal models.
Klöting
I
I
Department of Laboratory Animal Science, University of Greifswald, Karlsburg, Germany.
Kovács
P
P
van den Brandt
J
J
D000818:Animals; D002875:Chromosomes; D003433:Crosses, Genetic; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D005838:Genotype; D006973:Hypertension / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D051381:Rats; D011918:Rats, Inbred SHR