title: Effects of lung preservation solutions on PMN activation in vitro.
abstract: Polymorphonuclear leukocyte (PMN) activation and PMN-endothelial cell interactions may cause graft failure due to ischemia-reperfusion injury after lung transplantation. We investigated the effects of Euro-Collins solution (EC), low-potassium dextran solution (LPD), and EC plus pentoxifylline (EC-PTXF) on adhesion molecule (CD11b/CD18 and L-selectin) expression, chemotaxis, and oxidative burst of PMN. PMN from healthy human volunteers were incubated with EC, LPD, and EC-PTXF, and, in controls, without preservation solution. LPD exerted a suppressive effect on PMN chemotaxis as compared to EC (P < 0.05), but had no attenuating effect on the increase of CD11b/CD18, the shedding of L-selectin, and intracellular oxidant generation. EC-PTXF attenuated the expression of CD11b/CD18 and the oxidative burst as compared to EC alone (P < 0.05). These effects of LPD and PTXF on PMN function may contribute to successful organ preservation in transplantation.
authors.affiliation: Department of medicine, National Cardiovascular Center, Osaka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Sakamaki
authors.forename: H
authors.initials: H
authors.lastname: Hoffmann
authors.forename: S
authors.initials: S
authors.lastname: Münzing
authors.forename: F
authors.initials: F
authors.lastname: Krombach
authors.forename: K
authors.initials: K
authors.lastname: Messmer
authors.forename: F W
authors.initials: FW
authors.lastname: Schildberg
mesh_terms: D018821:CD18 Antigens / Q000235:genetics; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003574:Cytochrome c Group / Q000378:metabolism; D003911:Dextrans / Q000494:pharmacology; D005786:Gene Expression Regulation; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D066298:In Vitro Techniques; D019041:L-Selectin / Q000235:genetics; D008168:Lung; D016177:Macrophage-1 Antigen / Q000235:genetics; D018375:Neutrophil Activation / Q000187:drug effects* / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010431:Pentoxifylline / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000097:blood
chemical_list: D018821:CD18 Antigens; D003574:Cytochrome c Group; D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D016177:Macrophage-1 Antigen; D019188:Organ Preservation Solutions; C083290:low potassium dextran glucose solution; D013481:Superoxides; D019041:L-Selectin; D005947:Glucose; D010431:Pentoxifylline