Endogenous porphyrin accumulation and photosensitization in the yeast Saccharomyces cerevisiae in the presence of 2,2'-dipyridyl.
The chelator 2,2'-dipyridyl (0.2 mM) induces a remarkable increase of protoporphyrin IX concentration as well as of its Zn-containing complex in the yeast Saccharomyces cerevisiae. Endogenous porphyrin accumulation results in five- to six-fold cell sensitization to visible light (400-600 nm). Mitochondria isolated from the cells grown in the presence of 2,2'-dipyridyl accumulate protoporphyrin IX and Zn-protoporphyrin IX, while plasma membranes besides that exhibit porphyrin-type fluorescence at 670-675 nm in chloroform extract. The protoporphyrin IX content increases more than four-fold in mitochondria and two-fold in plasma membranes isolated from chelator-treated cells. The relative contribution of subcellular structure photodestruction to photoinduced cell inactivation is discussed.
Strakhovskaya
M G
MG
Department of Biophysics, Faculty of Biology, M.V. Lomonosov Moscow State University, Russia.
Shumarina
A O
AO
Fraikin GYa
Rubin
A B
AB
D015082:2,2'-Dipyridyl / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D008027:Light; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects
D017319:Photosensitizing Agents; D011166:Porphyrins; D015082:2,2'-Dipyridyl