The distribution of gentamicin in the rabbit cornea following iontophoresis to the central cornea.
The purpose of this study was to evaluate the penetration of gentamicin into the central, midperipheral and peripheral cornea of rabbits following iontophoresis to the central 3 mm of the cornea. Four groups (groups 1-4) of five rabbits (one eye per rabbit) underwent corneal iontophoresis using gentamicin dissolved in agar. Low (1 mg/ml) and high (10 mg/ml) concentrations of gentamicin in agar were used for one or ten minutes. Two control groups (groups 5 and 6) of five eyes each underwent mock iontophoresis with low and high concentrations of agar-gentamicin mixture. Following sacrifice of the rabbits, the central, midperipheral and peripheral parts of each cornea were excised. Gentamicin concentration was determined in each part of every cornea. High concentrations of gentamicin (951.6 +/- 369.4 microg/ml to 26.6 +/- 41.34 microg/ml) were obtained in the central parts of all the iontophoresis-treated corneas. In each group, except group 6, central corneas had higher concentrations of gentamicin compared to midperipheral corneas (p = 0.038 to p = 0.021), and midperipheral corneas had higher levels than peripheral corneas (p = 0.038 to p = 0.021). Following iontophoresis, gentamicin is found in all portions of the corneas; however, the highest concentration of the drug remains in the central cornea.
Frucht-Pery
J
J
Department of Ophthalmology, The Brown School of Public Health and Community Medicine, Hadassah University Hospital, Jerusalem, Israel.
Goren
D
D
Solomon
A
A
Siganos
C S
CS
Mechoulam
H
H
Solomon
A
A
Ever-Hadani
P
P
Shapiro
M
M
D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D003315:Cornea / Q000378:metabolism*; D005839:Gentamicins / Q000493:pharmacokinetics*; D007478:Iontophoresis; D011817:Rabbits; D014018:Tissue Distribution
D000900:Anti-Bacterial Agents; D005839:Gentamicins