Expression of cathepsin B-like enzyme activity in cell lysate from cultured human normal keratinocytes.
In the present study, we have found that the cell lysate from cultured human normal keratinocytes from foreskin (HFKs) hydrolyzed alpha-N-benzoyl-DL-arginine beta-naphthylamide (BANA), and the BANA hydrolysis occurred most under conditions of 37 degrees C and pH 6.0. This activity was strongly inhibited by leupeptin, which is an inhibitor to cathepsin B. These results suggested that the cell lysate from cultured HFKs contained cathepsin B-like enzyme activity. This is the first report to demonstrate that cathepsin B-like enzyme activity was expressed in the cell lysate from human normal keratinocytes.
Ohno
T
T
Department of Dermatology, Shimane Medical University.
Hayashi
S
S
Takata
Y
Y
Ueda
D
D
Dekio
S
S
D001587:Benzoylarginine-2-Naphthylamide / Q000378:metabolism; D002401:Cathepsin B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D015603:Keratinocytes / Q000201:enzymology*; D007976:Leupeptins / Q000494:pharmacology; D008297:Male
D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D001587:Benzoylarginine-2-Naphthylamide; D002401:Cathepsin B; C032854:leupeptin