title: Activation of DNA replication in yeast by recruitment of the RNA polymerase II transcription complex.
abstract: Activators of transcription are known to also play an important and direct role in activating DNA replication. However, the mechanism whereby they stimulate replication has remained elusive. One model suggests that, in the context of replication origins, transcriptional activators work by interacting with replication factors. We show that a defined, single interaction between a DNA-bound derivative of the activator Gal4 and Gal11P, a mutant form of the RNA polymerase II holoenzyme component Gal11, suffices for stimulating DNA replication as it does for transcription. Moreover, recruitment of TBP, which can activate transcription from a gene promoter, also stimulates DNA replication from an origin site. These results strongly argue that transcriptional activators may not necessarily need to contact DNA replication factors directly, but can stimulate replication by recruiting the RNA polymerase II transcription complex to DNA.
authors.affiliation: Institute for Veterinary Biochemistry, University of Zürich, Switzerland.
authors.forename: I
authors.initials: I
authors.lastname: Stagljar
authors.forename: U
authors.initials: U
authors.lastname: Hübscher
authors.forename: A
authors.initials: A
authors.lastname: Barberis
mesh_terms: D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D005800:Genes, Fungal; D012319:RNA Polymerase II / Q000378:metabolism*; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D012319:RNA Polymerase II