[Effects of nordihydroguaiaretic acid on the growth and differentiation of SHG-44 glioma cell line].
OBJECTIVE
To investigate the effects of nordihydroguaiaretic acid (NDGA) on the growth and differentiation of glioma cells and their mechanism.


METHODS
A human glioblastoma cell line (SHG-44) was treated with NDGA in the medium or intracytoplasmic microinjection of NDGA. Changes of the growth, morphology, cell cycle and immunohistochemical features of the cell were investigated.


RESULTS
It was found that the growth rates and proliferation activity were inhibited by 100 mumol/L NDGA in the medium, with cell cycles altered. NDGA could induce differentiation of these cells with the glial filaments increased, and the treated cells expressed more GFAP but less vimentin. The expression of p53 and bFGF were also decreased. Furthermore, the result of microinjection (1.5 x 10(-11) g/cell) showed similar but more rapid and permanent effects on the glioma cells.


CONCLUSIONS
NDGA has multiple effects of growth inhibition, differentiation therapy, as well as angiosuppression on human glioblastoma cells.
Bian
X
X
Department of Pathology, Third Military Medical University, Chongqing.
Shi
J
J
Xin
R
R
D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D009637:Masoprocol / Q000494:pharmacology*; D008845:Microinjections; D014407:Tumor Cells, Cultured
D016859:Lipoxygenase Inhibitors; D009637:Masoprocol