title: Interaction of eukaryotic initiation factor eIF4B with the internal ribosome entry site of foot-and-mouth disease virus is independent of the polypyrimidine tract-binding protein.
abstract: Eukaryotic translation initiation factor 4B (eIF4B) binds directly to the internal ribosome entry site (IRES) of foot-and-mouth disease virus (FMDV). Mutations in all three subdomains of the IRES stem-loop 4 reduce binding of eIF4B and translation efficiency in parallel, indicating that eIF4B is functionally involved in FMDV translation initiation. In reticulocyte lysate devoid of polypyrimidine tract-binding protein (PTB), eIF4B still bound well to the wild-type IRES, even after removal of the major PTB-binding site. In conclusion, the interaction of eIF4B with the FMDV IRES is essential for IRES function but independent of PTB.
authors.affiliation: Institute of Biochemistry, D-35392 Giessen, Germany.
authors.forename: R C
authors.initials: RC
authors.lastname: Rust
authors.forename: K
authors.initials: K
authors.lastname: Ochs
authors.forename: K
authors.initials: K
authors.lastname: Meyer
authors.forename: E
authors.initials: E
authors.lastname: Beck
authors.forename: M
authors.initials: M
authors.lastname: Niepmann
mesh_terms: D005537:Aphthovirus / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D005057:Eukaryotic Cells; D039642:Eukaryotic Initiation Factors; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism*; D038941:Polypyrimidine Tract-Binding Protein; D014176:Protein Biosynthesis; D012367:RNA, Viral; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D039642:Eukaryotic Initiation Factors; D010448:Peptide Initiation Factors; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C031799:eIF-4B; D038941:Polypyrimidine Tract-Binding Protein