title: Activation of the 2'-N-acetyltransferase gene [aac(2')-Ia] in Providencia stuartii by an interaction of AarP with the promoter region.
abstract: The aac(2')-Ia gene in Providencia stuartii encodes a 2'-N-acetyltransferase capable of acetylating both peptidoglycan and certain aminoglycoside antibiotics. Regulation of the aac(2')-Ia gene is influenced in a positive manner by the product of the aarP gene, which encodes a small transcriptional activator of the AraC (XylS) family. In this study, we demonstrate the sequence requirements at the aac(2')-Ia promoter for AarP binding and activation.
authors.affiliation: Departments of Medicine and of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Macinga
authors.forename: M R
authors.initials: MR
authors.lastname: Paradise
authors.forename: M M
authors.initials: MM
authors.lastname: Parojcic
authors.forename: P N
authors.initials: PN
authors.lastname: Rather
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D001426:Bacterial Proteins; D015971:Gene Expression Regulation, Enzymologic; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000302:isolation & purification / Q000502:physiology*
chemical_list: C093833:AarP protein, Providencia stuartii; D001426:Bacterial Proteins; D015534:Trans-Activators; D000123:Acetyltransferases