title: Structure of aspartate-beta-semialdehyde dehydrogenase from Escherichia coli, a key enzyme in the aspartate family of amino acid biosynthesis.
abstract: Aspartate beta-semialdehyde dehydrogenase (ASADH) lies at the first branch point in an essential aspartic biosynthetic pathway found in bacteria, fungi and the higher plants. Mutations in the asd gene encoding for ASADH that produce an inactive enzyme are lethal, which suggests that ASADH may be an effective target for antibacterial, herbicidal and fungicidal agents. We have solved the crystal structure of the Escherichia coli enzyme to 2.5 A resolution using single isomorphous replacement and 3-fold non-crystallographic symmetry. Each monomer has an N-terminal nucleotide-binding domain and a dimerisation domain. The presence of an essential cysteine locates the active site in a cleft between the two domains. The functional dimer has the appearance of a butterfly, with the NADP-binding domains forming the wings and the dimerisation domain forming the body.A histidine residue is identified as a likely acid/base catalyst in the enzymic reaction. Other amino acids implicated in the enzymic activity by mutagenesis are found in the active site region and define the substrate binding pocket.
authors.affiliation: Department of Biochemistry Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, 02254-9110, USA. A.T.Hadfield@bris.ac.uk
authors.forename: A
authors.initials: A
authors.lastname: Hadfield
authors.forename: G
authors.initials: G
authors.lastname: Kryger
authors.forename: J
authors.initials: J
authors.lastname: Ouyang
authors.forename: G A
authors.initials: GA
authors.lastname: Petsko
authors.forename: D
authors.initials: D
authors.lastname: Ringe
authors.forename: R
authors.initials: R
authors.lastname: Viola
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000096:biosynthesis; D001223:Aspartate-Semialdehyde Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D001224:Aspartic Acid / Q000096:biosynthesis*; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D000596:Amino Acids; D001224:Aspartic Acid; D009249:NADP; D001223:Aspartate-Semialdehyde Dehydrogenase