Bronchial allergen challenge in subjects with low levels of allergic sensitization to indoor allergens.
BACKGROUND
Low skin reactivity to common inhalant allergens is frequently found in asymptomatic individuals as well as in patients with respiratory complaints. However, most studies on bronchial allergen challenge concern patients with high levels of allergic sensitization. The present study was directed to bronchial reactions after allergen challenge in subjects with low skin reactivity to Dermatophagoides pteronyssinus or cat dander.


METHODS
Titrated intracutaneous skin tests, skin prick tests, specific IgE assays, histamine release on washed leukocytes, and bronchial histamine and allergen-challenge tests were performed in 20 subjects with an intracutaneous skin test threshold for cat dander (Felis domesticus) or D. pteronyssinus above 0.1 BU/ml (mean wheal diameter in skin prick test with 10000 BU/ml: 4.4mm). Ten of the 20 patients had specific IgE below the detection limit in at least one of the three IgE assays which were done. Fifteen patients had a specific IgE level below 2 kU/I in all three tests. As a positive control group, the same parameters were studied in seven moderately sensitized patients with an intracutaneous skin test threshold below 0.1 BU/ml (mean wheal diameter with 10000 BU/ml: 7.2mm).


RESULTS
The 20 subjects with low levels of allergic sensitization had an early decrease in FEV1 of 8.6% (P<0.01) and a mean late decrease of 6.3% (P<0.05). There was a trend for decrease in PC20 histamine 24h after allergen challenge (-0.4 doubling doses, P=0.09).


CONCLUSIONS
In this group of subjects with low levels of allergic sensitization, a statistically significant early and late decrease in FEV1 was found. However, the decrease in lung function was small and unnoticed by most patients. The increase in nonspecific bronchial hyperresponsiveness after bronchial allergen challenge did not reach statistical significance in the study group. The results indicate that allergen exposure in patients with low levels of allergic sensitization may lead to airways changes in the absence of acute symptoms.
Witteman
A M
AM
Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands.
Mulder
M
M
Aalberse
R C
RC
Jansen
H M
HM
van der Zee
J S
JS
D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology; D001985:Bronchial Provocation Tests; D002415:Cats / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D011852:Radioallergosorbent Test; D012129:Respiratory Function Tests; D012220:Rhinitis / Q000276:immunology; D012882:Skin Tests
D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007073:Immunoglobulin E; D006632:Histamine