Substratum of pleiotrophin (HB-GAM) stimulates rat CG-4 line oligodendrocytes to adopt a bipolar morphology and disperse: primary O-2A progenitor glial cells disperse similarly on pleiotrophin.
Pleiotrophin (HB-GAM), an extracellular matrix-associated protein with a high content of basic amino acid residues, is expressed in the central nervous system during late pre- and early post-natal development and promotes neurite outgrowth in vitro. Here, we show that, on a substratum of pleiotrophin formed from a 5 or 10 microg/ml solution, undifferentiated rat CG-4 line oligodendrocytes adopt a bipolar morphology and disperse over the substratum, as we have previously shown with poly-L-lysine (Rumsby et al. Neurosci. Res. Commun. 23:101-109, 1998). On pleiotrophin substrata formed from coating solutions of 1 microg/ml and below, CG-4 line cells form aggregates and do not disperse, as is also the case with poly-L-lysine. The same dispersing effect is observed with rat primary 0-2A progenitor glial cells on pleiotrophin substrata from solutions of 5 and 10 microg/ml: 0-2A cells aggregate together on pleiotrophin substrata formed from lower concentrations and do not disperse. A pleiotrophin substratum enhances proliferation of CG-4 line oligodendrocytes and primary 0-2A progenitor glial cells. The results show that pleiotrophin provides a substratum that can influence progenitor oligodendrocyte morphology, aid cell dispersion, and perhaps also enhance progenitor oligodendrocyte cell growth.
Rumsby
M
M
Department of Biology, University of York, England. mgr1@york.ac.uk
Suggitt
F
F
Haynes
L
L
Hughson
E
E
Kidd
D
D
McNulty
S
S
D000818:Animals; D015415:Biomarkers; D002352:Carrier Proteins / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line; D016764:Cell Polarity; D016207:Cytokines / Q000494:pharmacology*; D005732:Gangliosides / Q000096:biosynthesis; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011107:Polylysine / Q000494:pharmacology*; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D015415:Biomarkers; D002352:Carrier Proteins; D016207:Cytokines; D005732:Gangliosides; C062875:ganglioside A2B5; C066845:pleiotrophin; D011107:Polylysine; C026226:ganglioside, GD3