Evolution of centromeric satellite DNA and its use in phylogenetic studies of the Sparidae family (Pisces, Perciformes).
In this paper, we use the EcoRI centromeric satellite DNA family conserved in Sparidae as a taxonomic and a phylogenetic marker. The analyses of 56 monomeric units (187 bp in size) obtained by means of cloning and PCR from 10 sparid species indicate that this repetitive DNA evolves by concerted evolution. Different phylogenetic inference methods, such as neighbor-joining and UPGMA, group the 56 repeats by taxonomic affinity and support the existence of at least two monophyletic groups within the Sparidae family. These results reinforce the recent taxonomic revision of the genera Sparus and Pagrus and contradict previous classifications of the Sparidae family.
Garrido-Ramos
M A
MA
Facultad de Ciencias, Universidad de Granada, Granada, 18071, Spain.
de la Herrán
R
R
Jamilena
M
M
Lozano
R
R
Ruiz Rejón
C
C
Ruiz Rejón
M
M
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D019143:Evolution, Molecular; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D016678:Genome; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment
D004276:DNA, Satellite; D005819:Genetic Markers; D015246:Deoxyribonuclease EcoRI