[Inhibitory effect of replication and expression of HDV by antisense oligodeoxynucleotides in H1 delta 9 cell].
OBJECTIVE
To study inhibitory effect of an antisense oligodeoxynucleotide (ASODN) and its phosphorothioate (S-ASODN) on replication and expression of HDV in H1 delta 9 cell.


METHODS
In previous studies, it was proved that the ASODNs which are complementary to genomic HDV ribozyme self-cleavage site and stem I regions can inhibit availably genomic HDV ribozyme activity. In the present study, a 15-mer ASODN and S-ASODS which are complementary to this region (nucleotide 684-698) were added to medium of cultured H1 delta 9 cell. HDAg and HDV cDNA were detected by ELISA and Dot blot hybridization.


RESULTS
Twenty-fourth hour after 6 mumol/L ASODN and S-ASODN were added, both the secreting amount of HDAg in the supernatant and HDV RNA were inhibited. The inhibiting rates were 76.14% and 84.50% respectively. When the concentration of S-ASODN was 2, 4, 6 mumol/L, the inhibiting rate showed the feature of dosage dependence. Inhibitory effect of ASODN and S-ASODN were similar in the same dosage.


CONCLUSIONS
The results suggest that ASODN and S-ASODN can availably inhibit replication and expression of HDV in H1 delta 9 cell.
Li
H
H
Center of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing.
Mao
Q
Q
Li
Q
Q
D003698:Hepatitis Delta Virus / Q000187:drug effects* / Q000502:physiology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012367:RNA, Viral / Q000037:antagonists & inhibitors; D013873:Thionucleotides / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D016376:Oligonucleotides, Antisense; D012367:RNA, Viral; D013873:Thionucleotides