Determination of the fraction composition of blood lipoproteins by the small-angle X-ray scattering technique.
A new method for analyzing the fraction composition of blood lipoproteins (LP) was developed based on the small-angle X-ray scattering (SAXS) technique. The method allows quantitative determination of the contents of basic LP fractions (high-density LP, low-density LP, very low-density LP and their subfractions) in the blood plasma or serum. The results of LP analysis by the new method were compared with electron microscopy, ultracentrifugation and gel electrophoresis data. The results obtained by SAXS correlated with those obtained by traditional methods. The new method for the determination of the LP fraction composition in the blood is rapid (1-1.5 h), uses only one reagent (e.g., sucrose) and features a high accuracy and resolution up to LP subfractions. A total of 0.05 ml of the blood plasma or serum is required for an assay. The assays can be carried out in purified preparations or in the blood plasma or serum. The method developed can be used in clinical practice for diagnostics and in scientific research.
Tuzikov
F V
FV
Institute of Molecular Biology, State Research Center of Virology and Biotechnology, Vector, Koltsovo, Novosibirsk Region, Russia.
Tuzikova
N A
NA
Panin
L E
LE
Nikitin
Y P
YP
Krylova
I N
IN
D002624:Chemistry, Clinical / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000648:ultrastructure; D008077:Lipoproteins, LDL / Q000648:ultrastructure; D008079:Lipoproteins, VLDL / Q000648:ultrastructure; D012542:Scattering, Radiation; D014965:X-Rays
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL