Selective expression of serotonin receptor transcripts in the mammalian cochlea and its subdivisions.
Expression of serotonin receptor (5-HTR) mRNA has been determined in the mammalian cochlea and its subdivisions by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Specific primers targeting individual 5-HTRs 1-7 directed amplification of 5-HTR subtypes 1A, 1B, 2B, 2C, 3, 5B, and 6 from mouse cochlea cDNA. No evidence of expression was obtained for 5-HTRs 1D, 2A, 4 (L and S), 5A, and 7. The distribution of receptor mRNA within the cochlea was determined with application of RT-PCR to morphologically defined microdissected subfractions of the rat cochlea. Messages for 5-HTR subtypes 1A, 1B, 2B, and 6 were present in the organ of Corti, lateral wall, and spiral ganglion subfractions. Messages for 5-HTR subtypes 2C, 3 and 5B were found in the spiral ganglion, but not in the organ of Corti or lateral wall fractions. The existence of transcripts for 5-HTRs 1A, 1B, 2B and 6 in the organ of Corti is consistent with a role for these receptors in serotonin-mediated modulation of the mechanosensory signal.
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C K
CK
Laboratory of Bio-otology, Department of Otolaryngology, Wayne State University, Detroit, MI, USA.
Drescher
M J
MJ
Hatfield
J S
JS
Drescher
D G
DG
D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D008297:Male; D008465:Mechanoreceptors / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009925:Organ of Corti / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011922:Rats, Mutant Strains; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000502:physiology; D013136:Spiral Ganglion / Q000378:metabolism
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D012701:Serotonin