title: The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans.
abstract: Osmoregulated periplasmic glucans (OPGs) of Escherichia coli are anionic oligosaccharides that accumulate in the periplasmic space in response to low osmolarity of the medium. Their anionic character is provided by the substitution of the glucosidic backbone by phosphoglycerol originating from the membrane phospholipids and by succinyl residues from unknown origin. A phosphoglycerol-transferase-deficient mdoB mutant was subjected to Tn5 transposon mutagenesis, and putative mutant clones were screened for changes in the anionic character of OPGs by thin-layer chromatography. One mutant deficient in succinylation of OPGs was obtained, and the gene inactivated in this mutant was characterized and named mdoC. mdoC, which encodes a membrane-bound protein, is closely linked to the mdoGH operon necessary for the synthesis of the OPG backbone.
authors.affiliation: Laboratoire de Chimie Biologique, UMR111 du CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq, Cedex, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Lacroix
authors.forename: E
authors.initials: E
authors.lastname: Lanfroy
authors.forename: V
authors.initials: V
authors.lastname: Cogez
authors.forename: Y
authors.initials: Y
authors.lastname: Lequette
authors.forename: A
authors.initials: A
authors.lastname: Bohin
authors.forename: J P
authors.initials: JP
authors.lastname: Bohin
mesh_terms: D002855:Chromatography, Thin Layer; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005838:Genotype; D005936:Glucans / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009876:Operon; D010743:Phospholipids / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D013386:Succinates / Q000378:metabolism*; D014882:Water-Electrolyte Balance
chemical_list: D005936:Glucans; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D011994:Recombinant Proteins; D013386:Succinates