Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae.
House dust mite allergen is thought to be a major cause of asthma. Characterization of these allergen molecules is therefore an important step for the development of effective diagnostic and therapeutic agents against mite-associated allergic disorders. Here we report molecular cloning and expression of the group 6 (chymotrypsin-like) allergen from the house dust mite, Dermatophagoides farinae. Sequencing analysis indicates that cloned cDNA, designated Der f 6, encodes a 279 amino acid polypeptide which conserves a primary structure characteristic for chymotrypsin-like serine proteases found in mammals. Recombinant Der f 6 expressed in Escherichia coli bound IgE in a pool made of 20 sera, and induced histamine release from patients' peripheral blood cells.
Kawamoto
S
S
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan. skawa@ipc.hiroshima-u.ac.jp
Mizuguchi
Y
Y
Morimoto
K
K
Aki
T
T
Shigeta
S
S
Yasueda
H
H
Wada
T
T
Suzuki
O
O
Jyo
T
T
Ono
K
K
D000485:Allergens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D004391:Dust; D004926:Escherichia coli / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D004391:Dust; D006023:Glycoproteins; D012697:Serine Endopeptidases