[Quantitation and heterogeneity of serum alpha 2-macroglobulin].
alpha 2-Macroglobulin (alpha 2M) is a major protease inhibitor in human plasma. In this study, serum alpha 2M was determined by means of immunoelectrophoresis (IEP), rocket immunoelectrophoresis (R-IEP), laser nephelometry (LN) and single radial immunodiffusion (SRID) in 133 subjects. The values obtained by these methods coincided well in most specimens, but some specimens showed disagreement. The sera of alpha 2M deficiency found by IEP were analyzed by the other methods. We found that R-IEP gave the results of alpha 2M deficiency most frequently while the other methods gave the results of normal alpha 2M level for some specimens. We studied on the presumption that the phenomenon is due to heterogeneity in molecular weight of alpha 2M. After alpha 2M was purified by affinity chromatography and gel filtration chromatography, we further employed gel-filtration on a high-performance liquid chromatography (HPLC) to isolate alpha 2M with different molecular size. Two peaks of immunoreactive alpha 2M were found by the gel-filtration HPLC. When we performed IEP for both portions, the first portion with higher molecular size showed larger rate of migration than the second portion. Serum alpha 2M quantitation with SRID is not appropriate because molecular weight influences on the determination by SRID. We conclude that R-IEP is best suitable for the detection of decrease of serum alpha 2M, although this method requires expertness of manipulation.
Mashiko
T
T
Department of Clinical Pathology, Kitasato University School of Medicine, Sagamihara.
Ichikawa
K
K
Jimbo
S
S
Tanaka
T
T
Kanoh
Y
Y
Ohtani
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000379:methods; D008875:Middle Aged; D008970:Molecular Weight; D009391:Nephelometry and Turbidimetry; D000511:alpha-Macroglobulins / Q000032:analysis* / Q000302:isolation & purification
D000511:alpha-Macroglobulins