Circadian-regulated expression of a nuclear-encoded plastid sigma factor gene (sigA) in wheat seedlings.
The activity of a light-responsive psbD promoter in plastids is known to be regulated by a circadian clock. However, the mechanism of the circadian regulation of the psbD light-responsive promotor, which is recognized by an Escherichia coli-type RNA polymerase, is not yet known. We examined the time course of mRNA accumulation of two E. coli-type RNA polymerase subunit genes, sigA and rpoA, under a continuous light condition after 12 h light/12 h dark entrainment. Accumulation of the sigA mRNA was found to be regulated by a circadian clock, while rpoA mRNA did not show any significant oscillation throughout the experiment.
Morikawa
K
K
Graduate School of Human and Environmental Studies, Kyoto University, Japan.
Ito
S
S
Tsunoyama
Y
Y
Nakahira
Y
Y
Shiina
T
T
Toyoshima
Y
Y
D000595:Amino Acid Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli; D017343:Genes, Plant; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012639:Seeds / Q000235:genetics; D016415:Sequence Alignment; D012808:Sigma Factor / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit