Promotion of ATP and S-140 to ribosome inactivation with camphorin, cinnamomin, and other RNA N-glycosidases.
OBJECTIVE
To study the effect of ATP and extra-ribosomal factors (S-140) on type I and type II RNA N-glycosidases in inactivating ribosome.


METHODS
The activity of ATP and S-140 was determined by characterization of R-fragment in gel. An improved two-step method of cell-free protein synthesis system was used to quantitate the requirements of S-140 in ribosome inactivation.


RESULTS
IC50 ratios of camphorin, gamma-momorcharin, luffin S, luffin A, trichosanthin (type I); and ricin, ricin A-chain; cinnamonin, cinnamomin A-chain (type II) between the absence and presence of ATP and S-140 were 3108, 151, 51, 45, 15; and 47, 7, 26, 12, respectively.


CONCLUSIONS
The ribosome-inactivating activity of type II ribosome-inactivating proteins, including intact protein and its A-chain, was promoted by ATP and S-140. Camphorin showed a significant difference from cinnamomin in need of ATP and S-140 for such promoting.
Ruan
J P
JP
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
Chen
W F
WF
Liu
W Y
WY
D000255:Adenosine Triphosphate / Q000378:metabolism*; D020418:Algal Proteins; D000818:Animals; D008099:Liver / Q000378:metabolism; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015978:Trichosanthin / Q000378:metabolism
D020418:Algal Proteins; D010940:Plant Proteins; D011506:Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C104406:camphorin protein, Cinnamomum camphora; C061930:cinnamomin, Phytophthora cinnamomi; D015978:Trichosanthin; D000255:Adenosine Triphosphate; D012276:Ricin; D013482:Superoxide Dismutase; D009699:N-Glycosyl Hydrolases