Identification and Expression Pattern of DNA Polymerase alpha Gene in a Marine Diatom, Skeletonema costatum.
: To investigate the potential of DNA polymerase alpha as a marker for DNA replication in phytoplankton, two gene fragments that showed a high degree of similarity with eukaryotic DNA polymerase alpha were cloned from two strains of a diatom, Skeletonema costatum (Greville) Cleve. The gene fragments amplified with the polymerase chain reaction were 397 and 396 bp in length, respectively. The deduced amino acid sequences showed 44% to 61% similarity to the corresponding regions of DNA polymerase alpha sequences of eukaryotic organisms ranging from yeast to humans. The similarity was especially high in three evolutionarily conserved regions within the amplified fragments. Further, hybridization patterns from Southern blotting confirmed that the amplified fragments were an integral part on the genome of S. costatum. In batch cultures abundant messenger of DNA polymerase alpha appeared in the late exponential phase and the early stationary phase. This pattern suggests that DNA polymerase alpha expression is associated with actively dividing cells.
Hwang
SP
Institute of Zoology, Academia Sinica, Nankang, Taipei 115-29, Taiwan, Republic of China
Wang
SK
Wei
SF
Cheng
LC
Chang
J