Gene expressions of antiinflammatory mediators in THR retrieved interfacial membranes.
We investigated gene expression of antiinflammatory mediators in the interfacial membranes retrieved at hip revision arthroplasty using reverse transcription-polymerase chain reaction (RT-PCR). Levels of RT-PCR products were compared with those of synovial tissue from patients with osteoarthrosis or rheumatoid arthritis. Antiinflammatory mediators such as type II interleukin (IL)-1 receptor, IL-4, IL-10, IL-1 receptor antagonist, and transforming growth factor-beta 1 (TGF-beta 1) were expressed in the interfacial membrane. In interfacial tissue, the level of IL-10 was lower, but that of the IL-1 receptor antagonist higher than in diseased synovial tissue.
Nabae
M
M
Department of Orthopaedic Surgery, Shiga University of Medical Science, Japan.
Inoue
K
K
Ushiyama
T
T
Hukuda
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression / Q000235:genetics* / Q000276:immunology*; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation; D018836:Inflammation Mediators / Q000032:analysis*; D053590:Interleukin 1 Receptor Antagonist Protein; D016753:Interleukin-10 / Q000032:analysis*; D015847:Interleukin-4 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D016348:Osseointegration / Q000235:genetics* / Q000276:immunology*; D010003:Osteoarthritis / Q000473:pathology; D011475:Prosthesis Failure; D017472:Receptors, Interleukin-1 / Q000032:analysis*; D012086:Reoperation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000032:analysis*; D013583:Synovial Membrane / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000032:analysis*
C507763:IL1RN protein, human; D018836:Inflammation Mediators; D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4