Adenovirus cellular receptor site recirculation of HeLa cells upon receptor-mediated endocytosis is not low pH-dependent.
HeLa cells were depleted of 75% of the available adenovirus type 2 specific cellular receptor sites (CRSs) by controlled attachment of viruses at a high multiplicity of infection. Upon removal of unattached viruses and further incubation, the cells were able to recycle the CRSs to 75% of the level of uninfected control cells within 5 h. The rate of virus receptor recycling was approx. 1 CRS x min-1 x cell-1. The existence of receptor recycling further strengthens the involvement of a total process of receptor-mediated endocytosis in adenovirus internalization. The recirculation process was neither affected by the lysosomotropic agents ammonium chloride and amantadine-HCl, nor by the ionophore monensin or the multifunctional weak-base amine chloroquine.
Everitt
E
E
Department of Microbiology, Lund University, Sweden.
Rodríguez
E
E
D000260:Adenoviruses, Human / Q000187:drug effects / Q000502:physiology*; D000547:Amantadine / Q000494:pharmacology; D000643:Ammonium Chloride / Q000494:pharmacology; D002738:Chloroquine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008985:Monensin / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology; D011991:Receptors, Virus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D013462:Sulfur Radioisotopes
D011991:Receptors, Virus; D013462:Sulfur Radioisotopes; C085301:adenovirus receptor; D000643:Ammonium Chloride; D002738:Chloroquine; D008985:Monensin; D003513:Cycloheximide; D008715:Methionine; D000547:Amantadine; D009550:Nigericin