title: Cloning and sequencing of an acetyl-CoA synthetase (ADP-forming) gene from the amitochondriate protist, Giardia lamblia.
abstract: A Giardia lamblia gene, Glacs, was cloned, sequenced and expressed in Escheria Coli. This gene codes for a 726 residue long acetyl-CoA synthetase (ADP-forming). This enzyme is responsible for the formation of acetate, a metabolic endproduct of G. lamblia. It is known from only two Type I amitochondriate eukaryotes, G. lamblia and Entamoeba histolytica and from the archaebacterium, Pyrococcus furiosus. With Glacs as query, homologous unidentified open reading frames were detected in the complete genomes of only a few archaebacteria and eubacteria. These form a new protein family present in all three domains of life, which probably plays a central role in the acyl-CoA metabolism but is of restricted taxonomic distribution.
authors.affiliation: The Rockefeller University, New York, NY 10021, USA. sanchel@rockvax.rockfeller.edu
authors.forename: L B
authors.initials: LB
authors.lastname: Sánchez
authors.forename: H G
authors.initials: HG
authors.lastname: Morrison
authors.forename: M L
authors.initials: ML
authors.lastname: Sogin
authors.forename: M
authors.initials: M
authors.lastname: Müller
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics*; D017931:DNA Primers; D016829:Giardia lamblia / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames
chemical_list: D017931:DNA Primers; D003066:Coenzyme A Ligases; C101958:acetate-CoA ligase (ADP-forming)