Molecular characterization of Mycobacterium bovis BCG: Romanian sub-strain.
The Romanian sub-strain used for BCG vaccine production was characterized by polymorphic GC-rich repetitive sequence (PGRS) restriction profile and IS6110 detection. For comparison, the Pasteur and Moscow Mycobacterium bovis BCG sub-strains, M. bovis AN5, and M. tuberculosis H37Rv were analyzed. The BCG sub-strains showed the same restriction profile for PGRS after Pvu II, BamH I and Sal I digestion. They could be distinguished from the strains M. bovis AN5 and M. tuberculosis H37Rv after Pvu II and Sal I digestion. Sal I can also distinguish between M. bovis AN5 and M. tuberculosis H37Rv. Digestion with Pvu II, Sal I, Sca I, Mlu I and BamH I gave identical profiles of IS6110 hybridization for the Romanian and Pasteur BCG substrains.
Balazs
D
D
Cantacuzino Institute, Bucharest, Romania.
Cojocaru
R
R
Damian
M
M
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000145:classification* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial