A model for the generation of low level chemiluminescence from microbiological growth media and its depletion by bacterial cells.
We present a model for the development of chemiluminescence (CL) in autoclaved liquid growth media, as they are used in microbiology for the culturing of microorganisms, or in other related Maillard systems. The model distinguishes between four different stages consisting of sugar fragmentation during heating, autooxidation of highly reducing fragmentation products, radical chain reactions leading to a peroxidation of the media, and finally the formation of excited states, energy transfer reactions and CL emission. The proposed model is also discussed in regard of a recently reported elimination of this CL in growing cultures of microorganisms and possible pathways for this interference are suggested.
Vogel
R
R
Institut für Mikrobiologie und Molekularbiologie, Universität Hohenheim, Stuttgart Hohenheim, Germany.
Süssmuth
R
R
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005609:Free Radicals; D008163:Luminescent Measurements; D015416:Maillard Reaction
D003470:Culture Media; D005609:Free Radicals