title: Cryoenzymic studies on an organized system: myofibrillar ATPases and shortening.
abstract: We have exploited cryoenzymology, first, to probe the product release steps of myofibrillar ATPase under relaxing conditions and, second, to define the conditions for studying the contractile process in slow motion. Cryoenzymology implies perturbation by temperature and by the antifreeze added to allow for work at subzero temperatures. Here, we studied myofibrillar shortening and ATPases by the rapid quench flow method over a wide temperature range (-15 to 30 degrees C) in two antifreezes, 40% ethylene glycol and 20% methanol. The choice of solvent and temperature was dictated by the purpose of the experiment. Ethylene glycol (40%) is suitable for investigating the kinetics of the products release steps which is difficult in water. In this cryosolvent, the myofibrillar ATPase is not activated by Ca2+ nor is there shortening, except under special conditions, i.e., Ca2+ plus strong rigor bridges [Stehle, R., Lionne, C., Travers, F., and Barman, T. (1998) J. Muscl. Res. Cell Motil. 19, 381-392]. By the use of the glycol, we show that at low Ca2+ the kinetics of the ADP release are much faster with myofibrils than with S1. On the other hand, the kinetics of the Pi release were very similar for the two materials. Therefore, we suggest that, upon Ca2+ activation, only the Pi release kinetics are accelerated. In 20% methanol, in the presence of Ca2+, myofibrils shortened at temperatures above -2 degrees C but not below. At a given temperature above -2 degrees C, both the shortening and ATPase rates were reduced by the methanol. The temperature dependences of the myofibrillar ATPases (+/-Ca2+) converged with a decrease in temperature: at 20 degrees C, Ca2+ activated 30-fold, but at -15 degrees C, only about 5-fold. We suggest that studies in methanol may open the way for an investigation of muscle contraction in slow motion and, further, to obtain thermodynamic information on the internal forces involved in the shortening process.
authors.affiliation: INSERM U128, IFR 24, CNRS, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Lionne
authors.forename: R
authors.initials: R
authors.lastname: Stehle
authors.forename: F
authors.initials: F
authors.lastname: Travers
authors.forename: T
authors.initials: T
authors.lastname: Barman
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D019855:Ethylene Glycol; D005615:Freezing; D000432:Methanol; D009119:Muscle Contraction; D009126:Muscle Relaxation; D009210:Myofibrils / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D016658:Psoas Muscles; D011817:Rabbits; D012997:Solvents; D013696:Temperature
chemical_list: D010710:Phosphates; D012997:Solvents; D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases; D009218:Myosins; D019855:Ethylene Glycol; D000432:Methanol