Protease-resistant and detergent-insoluble prion protein is not necessarily associated with prion infectivity.
PrPSc, an abnormal isoform of PrPC, is the only known component of the prion, an agent causing fatal neurodegenerative disorders such as bovine spongiform encephalopathy (BSE) and Creutzfeldt-Jakob disease (CJD). It has been postulated that prion diseases propagate by the conversion of detergent-soluble and protease-sensitive PrPC molecules into protease-resistant and insoluble PrPSc molecules by a mechanism in which PrPSc serves as a template. We show here that the chemical chaperone dimethyl sulfoxide (Me2SO) can partially inhibit the aggregation of either PrPSc or that of its protease-resistant core PrP27-30. Following Me2SO removal by methanol precipitation, solubilized PrP27-30 molecules aggregated into small and amorphous structures that did not resemble the rod configuration observed when scrapie brain membranes were extracted with Sarkosyl and digested with proteinase K. Interestingly, aggregates derived from Me2SO-solubilized PrP27-30 presented less than 1% of the prion infectivity obtained when the same amount of PrP27-30 in rods was inoculated into hamsters. These results suggest that the conversion of PrPC into protease-resistant and detergent-insoluble PrP molecules is not the only crucial step in prion replication. Whether an additional requirement is the aggregation of newly formed proteinase K-resistant PrP molecules into uniquely structured aggregates remains to be established.
Shaked
G M
GM
Department of Neurology, Hadassah University Hospital, Jerusalem 91120, Israel.
Fridlander
G
G
Meiner
Z
Z
Taraboulos
A
A
Gabizon
R
R
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003902:Detergents; D004121:Dimethyl Sulfoxide; D019286:Endopeptidase K / Q000378:metabolism; D008854:Microscopy, Electron; D018622:PrP 27-30 Protein / Q000737:chemistry; D018620:PrPSc Proteins / Q000737:chemistry* / Q000472:pathogenicity; D011328:Prions / Q000737:chemistry* / Q000472:pathogenicity; D011487:Protein Conformation; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D012608:Scrapie / Q000378:metabolism; D012995:Solubility; D013388:Succinimides / Q000737:chemistry
D003902:Detergents; D018620:PrPSc Proteins; D011328:Prions; D013388:Succinimides; D018622:PrP 27-30 Protein; C025231:sarkosyl; D019286:Endopeptidase K; C019358:disuccinimidyl suberate; D004121:Dimethyl Sulfoxide; D012521:Sarcosine