Damage to hemoglobin by radiation-generated serum albumin radicals.
We have studied the effects of the interaction of radiation generated human serum albumin radicals (HSA*) with human hemoglobin molecules (Hb). Diluted Hb aqueous solutions were irradiated under N2O or argon without HSA and in the presence of HSA. Analysis of Hb absorbance spectra in the visible range, cross-linking of HSA* radicals with Hb molecules and functional properties of Hb were investigated. The degree of Hb destruction estimated on the basis of changes in the absorption spectra indicated that the effectiveness of HSA* radicals generated under N2O for Hb destruction was approximately equal to that of *OH radicals. In this case mainly *OH radicals formed the secondary HSA* radicals. However, during the irradiation Hb + HSA under argon the presence of equivalent amounts of oxidizing and reducing products of water radiolysis lowers the degree of Hb destruction. Some reactions of HSA* radicals with Hb molecules lead to the formation of covalent bonds between the molecules of both proteins. The following types of hybrids could be distinguished: Hb monomer-HSA, Hb dimer-HSA and higher aggregates. Structural changes of Hb by HSA* radicals were reflected by alterations in the oxygen affinity (increase) and cooperativity (decrease) of Hb. The results obtained indicate that in the experimental systems studied, the HSA* radical reactions with Hb molecules are favoured over recombination reactions of HSA* radicals. On this basis one can suggest that in the studied systems Hb plays the role of an acceptor of radical energy located on HSA.
Puchała
M
M
Department of Molecular Biophysics, University of Lódź, Poland.
Szweda-Lewandowska
Z
Z
D001665:Binding Sites; D003432:Cross-Linking Reagents; D019281:Dimerization; D005609:Free Radicals / Q000528:radiation effects; D006454:Hemoglobins / Q000737:chemistry* / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D011487:Protein Conformation / Q000528:radiation effects; D012709:Serum Albumin / Q000737:chemistry* / Q000528:radiation effects*; D013053:Spectrophotometry; D014867:Water
D003432:Cross-Linking Reagents; D005609:Free Radicals; D006454:Hemoglobins; D012709:Serum Albumin; D014867:Water