Linkage mapping of rat chromosome 5 markers generated from chromosome-specific libraries.
Seventy-six novel microsatellite markers with various simple sequence repeat (SSR) motifs are reported in this paper. They were generated on the basis of non-radioactive library screening procedures from flow-sorted rat Chromosome (Chr) 5-specific DNA, and were mapped in three rat backcross populations. Fifty-four of these markers mapped to Chr 5, while the other 22 mapped to other chromosomes of the rat genome. The marker D3Uwm8 is a new microsatellite marker for the rat syndecan 4 (ryudocan) gene. A genotyping protocol based on agarose gel electrophoresis is also provided in this paper.
Lan
H
H
McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA.
Shepel
L A
LA
Haag
J D
JD
Gould
M N
MN
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains
D005819:Genetic Markers