title: Zebrafish genetic map with 2000 microsatellite markers.
abstract: The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development. These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1.2-cM average resolution. One centimorgan in zebrafish is about 0. 74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks.
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
authors.forename: N
authors.initials: N
authors.lastname: Shimoda
authors.forename: E W
authors.initials: EW
authors.lastname: Knapik
authors.forename: J
authors.initials: J
authors.lastname: Ziniti
authors.forename: C
authors.initials: C
authors.lastname: Sim
authors.forename: E
authors.initials: E
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Kaplan
authors.forename: D
authors.initials: D
authors.lastname: Jackson
authors.forename: F
authors.initials: F
authors.lastname: de Sauvage
authors.forename: H
authors.initials: H
authors.lastname: Jacob
authors.forename: M C
authors.initials: MC
authors.lastname: Fishman
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D015723:Gene Library; D008040:Genetic Linkage; D016678:Genome; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation; D020161:Physical Chromosome Mapping; D015027:Zebrafish / Q000235:genetics*