title: Mutations in catabolite control protein CcpA separating growth effects from catabolite repression.
abstract: Carbon catabolite repression in Bacillus megaterium is mediated by the transcriptional regulator CcpA. A chromosomal deletion of ccpA eliminates catabolite repression and reduces the growth rate on glucose. We describe four single-amino-acid mutations in CcpA which separate the growth effect from catabolite repression, suggesting distinct regulatory pathways for these phenotypes.
authors.affiliation: Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Küster
authors.forename: T
authors.initials: T
authors.lastname: Hilbich
authors.forename: M K
authors.initials: MK
authors.lastname: Dahl
authors.forename: W
authors.initials: W
authors.lastname: Hillen
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001410:Bacillus megaterium / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D004794:Enzyme Repression; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014994:Xylose / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D014994:Xylose; D005947:Glucose