title: Induction of human leukocyte antigen (HLA)-A2-restricted and MAGE-3-gene-derived peptide-specific cytolytic T lymphocytes using cultured dendritic cells from an HLA-A2 esophageal cancer patient.
abstract: OBJECTIVE
  Using peripheral blood mononuclear cells (PBMCs) from a 10-year survivor with established human leukocyte antigen (HLA)-A2(+) and MAGE-3(+) esophageal cancer cell line (KYSE-170), we examined the induction of HLA-A2-restricted and MAGE-3-gene-derived peptide (FLWGPRALV, amino acids 271-279)-specific cytolytic T lymphocytes (CTLs).
  
  
  METHODS
  Autologous dendritic cells (DCs) cultured with granulocyte-macrophage colony stimulating factor and interleukin-4 were used as antigen presenting cells. PBMCs were stimulated by peptide-pulsed DCs in vitro.
  
  
  RESULTS
  PBMC cocultured with FLWGPRALV-pulsed DCs could induce the relevant peptide-specific CTLs, which had tumor necrosis factor production and specific cytotoxicity against relevant peptide-pulsed autologous DCs (34%, effector:target ratio = 40:1). Moreover, they showed specific cytotoxicity against the autologous esophageal cancer cell line KYSE-170 (17%, effector:target ratio = 40:1).
  
  
  CONCLUSIONS
  These results suggest that FLWGPRALV-pulsed cultured DCs would be a potent candidate for peptide vaccine against HLA-A2(+) and MAGE-3(+) esophageal cancer.
authors.affiliation: Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan. kanaoka@kuhp.kyoto-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Kanaoka
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: T
authors.initials: T
authors.lastname: Okino
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: A
authors.initials: A
authors.lastname: Otaka
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: M
authors.initials: M
authors.lastname: Imamura
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D000951:Antigens, Neoplasm; D003713:Dendritic Cells / Q000276:immunology; D004938:Esophageal Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D015789:HLA-A2 Antigen; D006801:Humans; D016233:Immunotherapy, Active; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D010455:Peptides; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; D036341:Intercellular Signaling Peptides and Proteins; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins; D010455:Peptides; C075525:A2-binding peptide