title: pH-dependent binding of Helicobacter pylori to pig gastric mucins.
abstract: A microtiter-based assay was developed to study the binding of Helicobacter pylori to pig gastric mucins purified by density-gradient centrifugation in CsCl/4 M guanidinium chloride. Binding of H. pylori was observed over the 'mucin' band as well as with 'low-density' components in the gradients, and binding to the latter was more pronounced when incubations were performed at 37 degrees C as compared to 20 degrees C. At a lower pH, binding of H. pylori (strain SVA 40) to the 'high-density' mucins from pig antrum was increased but binding to the 'low-density' ones was decreased. Binding of the P466 strain (Le(b)-specific) was mainly associated with the 'mucin' band, whereas the MO19 strain reacted preferentially with the 'low-density' components. In summary, H. pylori may bind to gastric mucins and the binding is influenced by temperature, pH and the repertoire of bacterial adhesins.
authors.affiliation: Mucosal Biology Group, CMB, Lund University, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Nordman
authors.forename: T
authors.initials: T
authors.lastname: Borén
authors.forename: J R
authors.initials: JR
authors.lastname: Davies
authors.forename: L
authors.initials: L
authors.lastname: Engstrand
authors.forename: I
authors.initials: I
authors.lastname: Carlstedt
mesh_terms: D000328:Adult; D000818:Animals; D001422:Bacterial Adhesion; D002499:Centrifugation, Density Gradient; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009077:Mucins / Q000378:metabolism*; D010437:Peptic Ulcer / Q000382:microbiology; D013552:Swine
chemical_list: D009077:Mucins