title: Cloning, sequencing, expression and characterization of the manganese superoxide dismutase gene from Vibrio alginolyticus.
abstract: The sodA gene coding for manganese superoxide dismutase from the marine microorganism Vibrio alginolyticus was cloned, sequenced and over-expressed in Escherichia coli using the pET20b (+) expression vector. The full-length gene was consisted of 603bp open reading frame, which encoded a polypeptide of 201 amino acid residues, with a calculated molecular weight of 22672Da. The deduced amino acid sequence of the sodA showed considerable homology to other Mn-SODs. The recombinant enzyme was efficiently purified from crude E. coli cell lysate by the metal ion affinity chromatography. The recombinant VAMn-SOD resisted thermo-denaturation up to 60 degrees C and was insensitive to inhibitors such as H2O2, NaN3 and diethyldithiocarbamic acid.
authors.affiliation: Institute of Marine Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, R. O. C.
authors.forename: Y C
authors.initials: YC
authors.lastname: Shyu
authors.forename: C C
authors.initials: CC
authors.lastname: Chiu
authors.forename: F P
authors.initials: FP
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015870:Gene Expression; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000235:genetics*; D013696:Temperature; D014733:Vibrio / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D013482:Superoxide Dismutase