title: CD 80(B7-1) expression on human tumor cell lines and its costimulatory signals for T cell proliferation and cytokine production.
abstract: OBJECTIVE
  To investigate CD 80 expression on human tumor cell lines and establish stable CD 80 expression transfectants to illustrate CD 80 costimulation on the T cell proliferation and cytokine production.
  
  
  METHODS
  Raji, MDA-453, MCF-7, Hela, 3AO, MKN-45 and EBV transformed B cell were detected for CD 80 expression by RT-PCR. CD 80 cDNA subcloned to retrovirus vector pLXSN, with them stable CD 80 transfectanants were established. With specific mAb BB1 and FACS assay, the expression of CD 80 were detected. Anti-CD3 induced T lymphocyte proliferation and IL-2, IFN-r production were performed to evaluate CD 80 costimulatory activity in the presence of calcium ionophore A23187 and phorbol ester PMA.
  
  
  RESULTS
  CD 80 (B7-1) expression of MDA-453, MCF-7, Hela, 3AO, MKN-45 was negative, and that of Raji and EBV transformed B cell was positive by RT-PCR and FACS methods. Cocultured with CD 80 transfected human breast carcinoma cell line MDA-453, anti-CD3 induced T cells proliferation and cytokine production were significantly increased in the presence of A23187 and PMA, but not with parental MDA-453.
  
  
  CONCLUSIONS
  CD 80 expression is absent on most human tumor cell line except for Raji and EBV transformed B cell. Full activation of T cell needs CD 80 costimulation. Influence of CD 80 costimulation on cytokine production is mainly regulated via IL-2 and IFN-r.
authors.affiliation: Shanghai Institute of Immunology, Shanghai Second Medical University, China.
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: B
authors.initials: B
authors.lastname: Ma
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Zhang
mesh_terms: D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002455:Cell Division; D005260:Female; D006367:HeLa Cells / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D018122:B7-1 Antigen; D018106:CD28 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D007371:Interferon-gamma