Preparation of encapsulated proteins dissolved in low viscosity fluids.
The majority of proteins are too large to be comprehensively examined by solution NMR methods, primarily because they tumble too slowly in solution. One potential approach to making the NMR relaxation properties of large proteins amenable to modern solution NMR techniques is to encapsulate them in a reverse micelle which is dissolved in a low viscosity fluid. Unfortunately, promising low viscosity fluids such as the short chain alkanes, supercritical carbon dioxide, and various halocarbon refrigerants all require the application of significant pressure to be kept liquefied at room temperature. Here we describe the design and use of a simple cost effective NMR tube suitable for the preparation of solutions of proteins encapsulated in reverse micelles dissolved in such fluids.
Ehrhardt
M R
MR
Johnson Research Foundation, Department of Biochemistry & Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6059, USA.
Flynn
P F
PF
Wand
A J
AJ
D002214:Capsules; D007202:Indicators and Reagents; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D012997:Solvents; D014783:Viscosity
D002214:Capsules; D007202:Indicators and Reagents; D008823:Micelles; D011506:Proteins; D012996:Solutions; D012997:Solvents