Synthesis of basement membrane by gastrointestinal cancer cell lines.
Gastrointestinal adenocarcinoma-derived cell lines were studied in order to determine their pattern of expression of basement membrane components and their ability to form a basement membrane. In contrast to the well-preserved expression of laminin beta 2, beta 3, gamma 1, and gamma 2 chain mRNAs, five of eight gastrointestinal cancer cells lacked alpha 3 mRNA. Immunohistochemical and electron microscopic examination of four cell lines transplanted subcutaneously to SCID mice demonstrated the presence of both alpha 3 and alpha 5 chains and the formation of a basal lamina in two cases. The other two cell lines lacked both alpha 3 and alpha 5 chains and could not form a basal lamina, suggesting that this deficiency may be a factor which affects their ability to form a basement membrane. This abnormality might play some role in stromal invasion by tumour cells in gastrointestinal cancer.
Akashi
T
T
Department of Pathology, Tokyo Medical and Dental University, Japan. akashi.path@med.tmd.jp
Miyagi
T
T
Ando
N
N
Suzuki
Y
Y
Nemoto
T
T
Eishi
Y
Y
Nakamura
K
K
Shirasawa
T
T
Osa
N
N
Tanaka
N
N
Burgeson
R E
RE
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism* / Q000648:ultrastructure; D015152:Blotting, Northern; D005770:Gastrointestinal Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D015870:Gene Expression; D006801:Humans; D007797:Laminin / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D016513:Mice, SCID; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D007797:Laminin; D009363:Neoplasm Proteins; D012333:RNA, Messenger