Effect of a single bolus of intracoronary basic fibroblast growth factor on perfusion in an ischemic porcine model.
Basic fibroblast growth factor (bFGF) has been shown to induce angiogenesis in various animal models, but the methods of administration used experimentally are not clinically feasible. The objective of this study was to determine whether a single intracoronary bolus injection of bFGF would improve coronary perfusion in a porcine ischemic model that mimics clinical chronic ischemia. A copper coil studded with gold was delivered into the proximal right coronary artery of juvenile Yorkshire pigs and deployed by interventional techniques. After a four-week interval for stenosis maturation, bFGF (100 micrograms) was administered by bolus injection into the left coronary artery in five animals, and vehicle alone was administered in four animals. Angiogenesis and change in right coronary perfusion area were assessed two weeks later by angiography, myocardial contrast echocardiography and immunohistochemistry. The right coronary perfusion area increased significantly after treatment in all but one of the animals that received bFGF but not in any of the controls. Intimal hyperplasia was not induced by bFGF. Capillary density determined histochemically was not different in the two groups. In conclusion, in a porcine ischemic model, bFGF administered by a single bolus intracoronary injection into the contralateral artery improved antegrade perfusion into the ischemic territory although without histological evidence of angiogenesis. This preliminary work merits further investigation.
Devlin
G P
GP
The Toronto Hospital, University of Toronto, Canada. devling@hwl.co.nz
Fort
S
S
Yu
E
E
Cusimano
R J
RJ
Wei
K
K
Rakowski
H
H
Butany
J
J
Richards
J A
JA
Sole
M J
MJ
Schwartz
L
L
D000818:Animals; D017023:Coronary Angiography; D003327:Coronary Disease / Q000000981:diagnostic imaging; D004195:Disease Models, Animal; D004452:Echocardiography; D005347:Fibroblasts; D006801:Humans; D017202:Myocardial Ischemia / Q000628:therapy; D015425:Myocardial Reperfusion / Q000379:methods*; D013552:Swine