Detection of equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) in Przewalski's wild horses.
In blood samples of seven captive equid species from four German zoos EHV-1 specific antibodies were detected in 76% and EHV-4 specific antibodies in 73% of the 55 animals, whereas 93% were tested positive for EHV-2 and EHV-5, respectively. In only one blood sample from a Przewalski's wild horse EHV-4 DNA was amplified by PCR. From seven Przewalski's wild horses EHV-2, and from another one EHV-5 was isolated by cocultivation. The identity of the virus isolates was verified by PCR and restriction enzyme digestion.
Borchers
K
K
Institut für Virologie, FU Berlin, Federal Republic of Germany.
Frölich
K
K
Ludwig
H
H
D000818:Animals; D000835:Animals, Wild; D000836:Animals, Zoo; D000914:Antibodies, Viral / Q000097:blood; D018397:Equidae / Q000821:virology*; D018142:Gammaherpesvirinae / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D005858:Germany; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D000914:Antibodies, Viral