[Aneuploidy detection by multi-color fluorescence in situ hybridization in mouse sperm].
Multi-color fluorescence in situ hybridization (FISH) was employed to evaluate the frequency of aneuploid sperm in young adult mice after treated by 2-(4'-thiazolyl) benzimidazole or thiabendazole (TB). The animals were given TB (100 200 300 mg/kg) by oral incubation daily for 11 days, and sacrificed 22 days after the last treatment of TB. Three-chromosome FISH was applied to determine the frequencies of diploid, disomic and nullisomic sperm with DNA probes specific for the chromosomes X, Y and 8. The aneuploid frequencies were not significantly different between the sperm in 100 mg/kg group and solvent control. The frequencies aneuploid and diploid sperm in middle dose group were significantly higher than the solvent control (P < 0.001-0.05). There were no significant aneuploid frequency increases were found in 300 mg/kg group excepting the frequency of diploid sperm. It is conclude that TB is a potential germinal aneugen in mammalian, which may block the cleavage during the meiosis. Epididymal sperm aneuploidy can be reliably determined by sperm FISH analysis in mice.
Wang
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X
Department of Life Sciences, Yunnan Normal University, Kunming.
Schmid
T
T
Adler
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ID
D000782:Aneuploidy; D000818:Animals; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013094:Spermatozoa / Q000648:ultrastructure*