title: Reactive oxygen intermediates stimulate interleukin-6 production in human bronchial epithelial cells.
abstract: Reactive oxygen intermediates (ROIs) play an important role in the initiation and progression of lung diseases. In this study, we investigated whether ROIs were involved in the induction of interleukin (IL)-6 in human bronchial epithelial cells. We exposed normal human bronchial epithelial cells as well as a human bronchial epithelial cell line, HS-24, to ROIs. We measured the amount of IL-6 in the culture supernatants using ELISA and the IL-6 mRNA levels using RT-PCR. Superoxide anions (O-2), but not hydrogen peroxide (H2O2), increased IL-6 production. To examine whether it is a cell type-specific mechanism of airway epithelial cells, the experiments were also performed in human lung fibroblasts, WI-38-40. In WI-38-40 cells, neither O-2 nor H2O2 increased IL-6 production. In contrast, tumor necrosis factor (TNF)-alpha (200 U/ml) induced IL-6 at the protein and mRNA levels in both airway epithelial cells and lung fibroblasts. This cytokine-induced IL-6 production was significantly suppressed by several antioxidants, including dimethyl sulfoxide (DMSO), in airway epithelial cells. In WI-38-40 cells, DMSO was not able to suppress IL-6 production induced by TNF-alpha. Pretreatment with DMSO recovered the TNF-alpha-induced depletion of intracellular reduced glutathione in HS-24 cells. These findings indicate that oxidant stress specifically induces IL-6 production in human bronchial epithelial cells and that in these cells ROIs may be involved in IL-6 production after stimulation with cytokines such as TNF-alpha. Presumably, ROIs participate in the local immune response in lung diseases via IL-6 release from bronchial epithelial cells.
authors.affiliation: The First Department of Internal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.forename: N
authors.initials: N
authors.lastname: Arai
authors.forename: R
authors.initials: R
authors.lastname: Hayashi
authors.forename: J
authors.initials: J
authors.lastname: Araya
authors.forename: S
authors.initials: S
authors.lastname: Matsui
authors.forename: N
authors.initials: N
authors.lastname: Yamashita
authors.forename: E
authors.initials: E
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D007425:Intracellular Membranes / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D000093242:Interleukin-6 Inhibitors
chemical_list: D000975:Antioxidants; D004121:Dimethyl Sulfoxide; D016166:Free Radical Scavengers; D005978:Glutathione; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors