Constitutive hsp70 is essential to mitosis during early cleavage of Paracentrotus lividus embryos: the blockage of constitutive hsp70 impairs mitosis.
Localization of constitutive hsp70 in eggs and early embryos of sea urchin Paracentrotus lividus is shown by means of in situ immunostaining. An accumulation of this protein is shown in the mitotic structures (asters, spindles and centrosomes). Microinjection of anti-hsp70 antibodies into eggs causes impairment of formation of mitotic structures and of cell division. This impairment goes from a complete mitotic block, to irregular mitotic apparatus formation with irregular cleavage, depending upon the antibody concentration. The localization of hsp70 after antibody microinjection is also described. Blockage of mitotic apparatus formation by nocodazole also blocks the concentration of hsp70 molecules observed in nontreated eggs. That the constitutive hsp70 plays a role in sea urchin mitosis is indicated.
Sconzo
G
G
Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Università di Palermo, Palermo, Italy. sconzo@unipa.it
Palla
F
F
Agueli
C
C
Spinelli
G
G
Giudice
G
G
Cascino
D
D
Geraci
F
F
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005306:Fertilization; D006131:Growth Inhibitors / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000502:physiology*; D008845:Microinjections; D008938:Mitosis / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D012617:Sea Urchins / Q000033:anatomy & histology / Q000196:embryology*; D013997:Time Factors
D006131:Growth Inhibitors; D018840:HSP70 Heat-Shock Proteins; D015739:Nocodazole