Mutations in catabolite control protein CcpA separating growth effects from catabolite repression.
Carbon catabolite repression in Bacillus megaterium is mediated by the transcriptional regulator CcpA. A chromosomal deletion of ccpA eliminates catabolite repression and reduces the growth rate on glucose. We describe four single-amino-acid mutations in CcpA which separate the growth effect from catabolite repression, suggesting distinct regulatory pathways for these phenotypes.
Küster
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Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Federal Republic of Germany.
Hilbich
T
T
Dahl
M K
MK
Hillen
W
W
D000483:Alleles; D000595:Amino Acid Sequence; D001410:Bacillus megaterium / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D004794:Enzyme Repression; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014994:Xylose / Q000378:metabolism
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D014994:Xylose; D005947:Glucose