Effect of L-arginine load on platelet aggregation: a comparison between normotensive and preeclamptic pregnant women.
BACKGROUND
In the present study we hypothesized that a derangement of the L-arginine-nitric oxide system could be involved in the development of the hypercoagulative status found during preeclampsia. In order to verify such hypothesis we have compared the effects of nitric oxide substrate, L-arginine on platelet aggregation. Moreover, we have also measured the L-citrulline plasma levels as a stochiometric metabolite resulting from the conversion L-arginine to nitric oxide.


METHODS
Nine preeclamptic women and 11 normotensive pregnant women were enrolled for the study. Subjects were infused with saline and with 30gr of L-arginine. Blood samples were drawn during the saline infusion (30 min), during L-arginine administration (30 min) and 30 min thereafter. ADP and collagen-induced platelet aggregation was studied as per Born with a dual-channel aggregometer (Chrono-Log, Mascia Brunelli, Italy) and L-citrulline was measured by HPLC.


RESULTS
In normotensive women the infusion significantly decreased ADP and collagen-induced aggregation after 15 minutes of L-arginine load; whereas no effects were observed in preeclamptic women. Similarly in normotensive but not in preeclamptic women L-arginine load was able to increase L-citrulline plasma levels.


CONCLUSIONS
In normotensive women the in vivo L-arginine administration decreases platelet aggregation with an increase of L-citrulline plasma levels. On the contrary, no effects were observed in preeclamptic women. These findings confirm that a hypercoagulative status characterizes preeclampsia and that such phenomenon could be explained by a derangement of the platelet L-arginine-nitric oxide pathway.
Facchinetti
F
F
Department of Obstetrics and Gynecology, University of Modena, Italy.
Neri
I
I
Piccinini
F
F
Marietta
M
M
Torelli
U
U
Bruschettini
P L
PL
Volpe
A
A
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001120:Arginine / Q000494:pharmacology*; D002956:Citrulline / Q000097:blood; D003094:Collagen / Q000494:pharmacology; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy
D010975:Platelet Aggregation Inhibitors; D002956:Citrulline; D009569:Nitric Oxide; D000244:Adenosine Diphosphate; D003094:Collagen; D001120:Arginine