Effect of Escherichia coli cytotoxic necrotizing factor 1 on repair of human bladder cell monolayers in vitro.
We hypothesized that Escherichia coli cytotoxic necrotizing factor 1 (CNF1) might impair migration or proliferation of bladder cells and could potentially interfere with repair of the bladder epithelium. Using experimentally wounded human T24 bladder epithelial cell monolayers as an in vitro model, we found that both the number of T24 cells and the maximum distance they migrated into wounded regions was significantly decreased by bacterial extracts containing E. coli CNF1.
Island
M D
MD
Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA. misland@clark.net
Cui
X
X
Warren
J W
JW
D001427:Bacterial Toxins / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003603:Cytotoxins / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006801:Humans; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology*; D014945:Wound Healing
D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; C060296:cytotoxic necrotizing factor type 1