Mutations affecting either generation or survival of cells influence the pool size of mature B cells.
The mature B cell compartment of MHC class II-deficient B6 I-Aalpha(-/-) and the btk-defective CBA/N mouse strain is 4- to 5-fold smaller than in wild-type B6 mice. The defect in B6 I-Aalpha(-/-) mice is intrinsic to B cells and due to a 4- to 5-fold reduced lifespan, which however can be normalized by an I-Ealpha(d) transgene, but only when expressed early during B cell development. The reduced number of mature B cells in the btk-defective CBA/N mouse is due to a 4- to 5-fold lower number of immature splenic B cells entering the mature compartment. The combined defects of reduced lifespan and impaired generation in double mutant mice result in a severe deficiency in the mature B cell pool.
Rolink
A G
AG
Basel Institute for Immunology, Switzerland. antibody@bii.ch
Brocker
T
T
Bluethmann
H
H
Kosco-Vilbois
M H
MH
Andersson
J
J
Melchers
F
F
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival; D006207:Half-Life; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains / Q000502:physiology*; D013154:Spleen / Q000166:cytology
D000949:Histocompatibility Antigens Class II