title: Cloning and characterization of an androgen-dependent acidic epididymal glycoprotein/CRISP1-like protein from the monkey.
abstract: A cDNA encoding an acidic epididymal glycoprotein (AEG)-like, CRISP1 (cysteine-rich secretory protein) protein from the monkey (Macaca mullata) epididymis has been cloned and sequenced. The monkey AEG (mAEG) has an open reading frame that encodes a protein containing 249 amino acids with a deduced molecular mass of 28 kDa. The mAEG protein sequence is 85% identical to human and 44% identical to mouse CRISP1, including all 16 conserved cysteine residues. mAEG also shows a significant amino acid homology with other CRISP proteins, rat AEG/DE, human TPX1/CRISP2, and guinea pig acrosomal autoantigen 1 (AA1). In addition, mAEG shows somewhat less homology to a toxin from the Mexican beaded lizard and to a human glioma pathogenesis-related protein. Northern blot analysis shows that the mRNA for mAEG is expressed in all the regions of the epididymis except the caput and was not detected in the testis, prostate, seminal vesicle, and brain. In castrated animals, mAEG gene expression in the epididymis is significantly diminished; however, testosterone enanthate replacement restored the normal level of expression, demonstrating that expression of mAEG is androgen dependent. Western blot analysis of monkey epididymal regions using mouse antirecombinant human AEG identified a 28-kDa protein only in the caudal region. Immunohistochemical analysis identified mAEG only in the principal cells of the cauda epididymal epithelium. Immunofluorescence analysis identified mAEG on the principal piece of the sperm tail and as small patches over the middle piece and head regions. The results described in the present study suggest that mAEG (CRISP1) is secreted in the monkey epididymis, regulated by androgens and present on epididymal spermatozoa.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, 27599-7090, USA.
authors.forename: P
authors.initials: P
authors.lastname: Sivashanmugam
authors.forename: R T
authors.initials: RT
authors.lastname: Richardson
authors.forename: S
authors.initials: S
authors.lastname: Hall
authors.forename: K G
authors.initials: KG
authors.lastname: Hamil
authors.forename: F S
authors.initials: FS
authors.lastname: French
authors.forename: M G
authors.initials: MG
authors.lastname: O'Rand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002369:Castration; D003001:Cloning, Molecular; D030501:Epididymal Secretory Proteins; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D008253:Macaca mulatta; D008297:Male; D008562:Membrane Glycoproteins; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013735:Testicular Hormones / Q000235:genetics*; D013739:Testosterone / Q000494:pharmacology
chemical_list: C486321:Crisp1 protein, mouse; C486320:Crisp3 protein, rat; D030501:Epididymal Secretory Proteins; D008562:Membrane Glycoproteins; D008667:Metalloproteins; D012471:Salivary Proteins and Peptides; D013735:Testicular Hormones; D013739:Testosterone