Reassessment of the role of CD8+ T cells in the induction of allograft tolerance by donor-specific blood transfusion.
Donor-specific allograft tolerance can be induced in adult rats by pregraft donor-specific blood transfusion (DST). We have previously shown that DST elicits in recipients the expansion of a donor-specific CD8+ T cell clone displaying the Vbeta18-Dbeta1-Jbeta2.7 TCR rearrangement, which rapidly infiltrates allografts after transplantation, suggesting a regulatory function for this clone in DST-induced tolerance. In this study, recipients were pretreated before grafting, using an anti-CD8 monoclonal antibody to deplete CD8+ T cells. CD8 depletion before DST and transplantation abrogated allograft tolerance, and the CD8+ T cell clone was absent from allografts. These effects were not observed when CD8 depletion was performed after DST but before transplantation. We conclude that CD8+ T cells play a role in the induction of allograft tolerance by DST.
Douillard
P
P
INSERM Unité U 437 Immunointervention dans les allo- et xeno-transplantations and Institut de Transplantation et de Recherche en Transplantation, Nantes, France.
Vignes
C
C
Josien
R
R
Chiffoleau
E
E
Heslan
J M
JM
Proust
V
V
Soulillou
J P
JP
Cuturi
M C
MC
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000961:Antilymphocyte Serum / Q000008:administration & dosage; D001483:Base Sequence; D001803:Blood Transfusion; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015342:DNA Probes / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology; D007108:Immune Tolerance / Q000235:genetics; D008212:Lymphocyte Depletion; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014019:Tissue Donors; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D015342:DNA Probes