Immobilized iminodiacetic acid (IDA)-type Cu2+ -chelating membrane affinity chromatography for purification of bovine liver catalase.
A metal ion chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined for the purification of bovine liver catalase (BLC). The effect of buffer pH, chelator surface density, initial concentration of crude enzyme and flow rate on BLC binding efficiency to the copper ion chelating membrane adsorbent were examined. Under the chromatographic conditions chosen, 67.7% recovery of BLC was attained with an overall 4.2-fold increase in specific activity in a single step. After performance of BLC purification, the chelating membrane adsorbent can be easily regenerated by imidazole or EDTA buffer with higher reviving effectiveness with the latter.
Yang
L
L
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, People's Republic of China.
Jia
L
L
Zou
H
H
Zhang
Y
Y
D000818:Animals; D002374:Catalase / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D007098:Imino Acids / Q000737:chemistry*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008854:Microscopy, Electron; D011485:Protein Binding
D002614:Chelating Agents; D007098:Imino Acids; D003300:Copper; D002374:Catalase; C008109:iminodiacetic acid