The electroreceptor organ of the catfish, Ictalurus melas, as a model for cisplatin-induced ototoxicity.
The ototoxic side-effects of the anti-cancer drug cisplatin (cis-diaminedichloroplatinum) have been widely investigated. However, the exact site of action remains unclear. In this study, the electroreceptor organ of the freshwater catfish Ictalurus melas is used as a model for examining the acute effects of cisplatin. The sensory cells in the electroreceptor organ are homologous to the inner hair cells in the cochlea of mammals. The effects of cisplatin administration can be investigated by in vivo recording of the spike trains from the electroreceptor organ primary afferents. Exposure of electroreceptor organs to 330 microM cisplatin for 1 h causes the spontaneous activity to drop, the overall sensitivity to diminish and the shape of the frequency characteristics to change. These effects persist in the week after administration. Control levels have returned at day 22. These results demonstrate an acute and, with considerable hysteresis, reversible cisplatin effect on the electroreceptor organs, which is to a large extent consistent with the cisplatin-induced effects in isolated hair cells in mammals. The time-course of the effect supports the hypotheses that ion channels are blocked immediately by cisplatin administration, and that cisplatin metabolites disturb enzymatic cellular processes.
Peters
R C
RC
Department of Comparative Physiology, Utrecht University, The Netherlands.
Mommersteeg
P M
PM
Heijmen
P S
PS
D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D004557:Electric Organ / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects; D007059:Ictaluridae; D008297:Male; D008322:Mammals; D012867:Skin / Q000294:innervation; D013997:Time Factors
D000970:Antineoplastic Agents; D002945:Cisplatin