Contribution of distinct adhesive interactions to platelet aggregation in flowing blood.
Aggregation of blood platelets contributes to the arrest of bleeding at sites of vascular injury, but it can occlude atherosclerotic arteries and precipitate diseases such as myocardial infarction. The bonds that link platelets under flow conditions were identified using confocal videomicroscopy in real time. Glycoprotein (GP) Ibalpha and von Willebrand factor (vWF) acted in synergy with alphaIIbbeta3 and fibrinogen to sustain platelet accrual at the apex of thrombi where three-dimensional growth resulted in increasing shear rates. The specific function of distinct adhesion pathways in response to changing hemodynamic conditions helps to explain hemostatic and thrombotic processes.
Ruggeri
Z M
ZM
Roon Research Center for Arteriosclerosis and Thrombosis, the Division of Experimental Hemostasis and Thrombosis, the Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA. ruggeri@scripps.edu
Dent
J A
JA
Saldívar
E
E
D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D005340:Fibrinogen / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D010973:Platelet Adhesiveness / Q000502:physiology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000502:physiology / Q000648:ultrastructure; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000502:physiology / Q000648:ultrastructure; D014841:von Willebrand Factor / Q000502:physiology / Q000648:ultrastructure
D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; D014841:von Willebrand Factor; D005340:Fibrinogen