Genetic variation and epidemiology of Echinococcus granulosus in Argentina.
Polymerase chain reaction-ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR-RFLP) analysis and sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1) genes were used to characterize 33 Echinococcus granulosus isolates collected from different regions and hosts in Argentina, and to determine which genotypes occurred in humans with cystic hydatid disease. The results of the study demonstrated the presence of at least 4 distinct genotypes; the common sheep strain (G1) in sheep from Chubut Province and in humans from Río Negro Province, the Tasmanian sheep strain (G2) in sheep and 1 human from Tucumán Province, the pig strain (G7) in pigs from Santa Fe Province and the carnel strain (G6) in humans from Río Negro and Buenos Aires Provinces. The finding that pigs harboured the pig strain and the occurrence of the Tasmanian sheep strain has considerable implications for the implementation of hydatid control programmes due to the shorter maturation time of both strains in dogs compared with the common sheep strain. Furthermore, this is the first report of the presence of the G2 and G6 genotypes in humans which may also have important consequences for human health.
Rosenzvit
M C
MC
Departamento de Parasitología Sanitaria, Administración Nacional de Laboratorios, Buenos Aires, Argentina.
Zhang
L H
LH
Kamenetzky
L
L
Canova
S G
SG
Guarnera
E A
EA
McManus
D P
DP
D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004443:Echinococcosis / Q000453:epidemiology* / Q000517:prevention & control; D004446:Echinococcus / Q000145:classification* / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D013552:Swine
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV