[The experimental study of the effect of insulin and danshen on culture of fibroblast in vitro].
To investigate the effect of Insulin and Danshen on anabolism and catabolism of collagen during the healing of wound, fibroblast which was cultured from human embryonic skin were divided into 3 groups: Insulin group, Danshen group and contral group. Each group was cultured for 2, 4 and 6 days, then the growth curve was established respectively.


RESULTS
1. The growth curve showed-insulin group > control group > Danshen group. 2. The rate of cell division in 3 groups was 19.6/1000, 2.5/1000, 3.77/1000 respectively. 3. The electron microscopic scanning showed that there was much of fibroid tissue surrounding the fibroblast cell in insulin group, but there was little fibroid tissue in Danshen group. The conclusion showed that insulin can accelerates proliferation of fibroblast and synthesis of collagen, but the effect of denshen was just on the contrary.
Liu
M
M
Department of Pediatric Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, P. R. China.
Peng
M
M
Liu
M
M
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D010618:Phenanthrolines / Q000494:pharmacology*; D010936:Plant Extracts; D027561:Salvia miltiorrhiza; D012867:Skin / Q000166:cytology*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007328:Insulin; D010618:Phenanthrolines; D010936:Plant Extracts; C028972:dan-shen root extract; D003094:Collagen