A new double-stranded RNA mycovirus from Botrytis cinerea.
A simple double-stranded RNA mycovirus was detected in a wild-type Botrytis cinerea 55k strain. The virus was located in the fungus cytoplasm as free particles of approximately 28 nm in diameter. The mycovirus possesses a single double-stranded genome segment of 1.8 kilobase pairs (kbp) encapsidated within an isometric protein coat whose main structural component is a polypeptide of 68 kDa. Cells infected with this virus showed an important degree of cellular degeneration.
Castro
M
M
Departamento de Ciencias Biológicas, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile.
Kramer
K
K
Valdivia
L
L
Ortiz
S
S
Benavente
J
J
Castillo
A
A
D020171:Botrytis / Q000648:ultrastructure / Q000821:virology*; D003593:Cytoplasm / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000302:isolation & purification; D014771:Virion / Q000302:isolation & purification
D012330:RNA, Double-Stranded