The use of polymerase chain reaction for determination of virulence factors of Escherichia coli strains isolated from pigs in Poland.
E. coli strains isolated from pigs with postweaning diarrhea or edema disease were tested by phenotypic and genotypic methods for the presence of virulence antigens and genes, respectively. The slide agglutination and ELISA analyses were used for determination of F4, F5, F6, F17, and F41 fimbriae whereas the prevalence of fimbrial fedA and toxin eltI, estI, estII, stx1, stx2 and stx2e genes were recorded by the means of PCR. Only F4 antigen (ac variant) was found in strains of the serogroup O149:K91 isolated from pigs with diarrhea. PCR analyses showed that the fedA gene encoding F18 fimbriae was present in 61.9% of strains isolated from pigs with diarrhea and in 84.2% of strains isolated from pigs with edema disease. The eltI genes encoding heat-labile toxin I (LTI) were present only in 9 out of 21 strains recovered from pigs with diarrhea. Shiga toxin 2 variant (stx2e) genes were found in six isolates from edema disease and also in one strain from diarrhea. The PCR test used in the study was a sensitive and valuable method for determination of virulence factors of E. coli strains.
Osek
J
J
National Veterinary Research Institute (PIWet.), Pulawy, Poland. josek@piwet.pulawy.pl
Gallien
P
P
Truszczyñski
M
M
Protz
D
D
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004768:Enterotoxins / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D006461:Hemolysis; D019081:O Antigens / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C030659:K antigens; D019081:O Antigens; C034151:heat stable toxin (E coli)