title: Characterization of methylglyoxal synthase from Clostridium acetobutylicum ATCC 824 and its use in the formation of 1, 2-propanediol.
abstract: A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The Km and Vmax values for the substrate dihydroxyacetone phosphate were 0.53 mM and 1.56 mmol min(-1) microgram(-1), respectively. When E. coli glycerol dehydrogenase was coexpressed with methylglyoxal synthase in E. coli BL21(DE3), 3.9 mM 1,2-propanediol was produced.
authors.affiliation: Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005-1892, USA.
authors.forename: K
authors.initials: K
authors.lastname: Huang
authors.forename: F B
authors.initials: FB
authors.lastname: Rudolph
authors.forename: G N
authors.initials: GN
authors.lastname: Bennett
mesh_terms: D000595:Amino Acid Sequence; D019757:Carbon-Oxygen Lyases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D019946:Propylene Glycol / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D019946:Propylene Glycol; D019757:Carbon-Oxygen Lyases; C021578:methylglyoxal synthase