Non-invasive measurement of chemotherapy drug concentrations in tissue: preliminary demonstrations of in vivo measurements.
Measurements of the tissue concentrations of two chemotherapy agents have been made in vivo on an animal tumour model. The method used is based on elastic scattering spectroscopy (ESS) and utilizes a fibre-optic probe spectroscopic system. A broadband light source is used to acquire data over a broad range of wavelengths and, therefore, to facilitate the separation of absorptions from various chromophores. The results of the work include measurements of the time course of the drug concentrations as well as a comparison of the optical measurements with high performance liquid chromatography (HPLC) analysis of the drug concentrations at the time of sacrifice. It is found that the optical measurements correlate linearly with HPLC measurements, but give lower absolute values.
Mourant
J R
JR
Bioscience & Biotechnology Group, Los Alamos National Laboratory, NM 87545, USA.
Johnson
T M
TM
Los
G
G
Bigio
I J
IJ
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis* / Q000493:pharmacokinetics; D055592:Biophysical Phenomena; D001703:Biophysics; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000032:analysis / Q000493:pharmacokinetics; D005069:Evaluation Studies as Topic; D005336:Fiber Optic Technology / Q000295:instrumentation; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009010:Monte Carlo Method; D009374:Neoplasms, Experimental / Q000378:metabolism; D019047:Phantoms, Imaging; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000032:analysis / Q000493:pharmacokinetics; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D014018:Tissue Distribution
D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D004317:Doxorubicin