TCR repertoire of suppressor CD8+CD28- T cell populations.
The cellular basis of graft rejection and the development of strategies for specific suppression of T cell responses against allogeneic and xenogeneic transplants represents an area of active investigation. Recently, a population of MHC-class I restricted CD8+CD28- T suppressor cells (Ts) which are able to inhibit specifically the proliferative response of allospecific, xenospecific and nominal-antigen specific CD4+ T helper cells (Th) has been identified. We have studied the TCR V beta gene repertoire expressed by CD8+CD28- Ts isolated from allospecific, xenospecific, and nominal antigen-specific T cell lines (TCL). A limited V beta repertoire has been found in all TCLs studied. The most restricted TCR V beta usage was observed within the population of Ts from xenospecific TCLs. The TCR V beta usage within the Ts subset of TCL differs from the TCR repertoire expressed by the CD4+ Th subset of the same TCL. This is consistent with the fact that Ts and Th cells recognize distinct MHC/ antigen complexes. The finding that the TCR repertoire used by Ts is limited opens new avenues for studying the mechanisms of transplant rejection.
Pennesi
G
G
Department of Pathology, College of Physicians and Surgeons of Columbia University, New York 10032, USA.
Liu
Z
Z
Ciubotariu
R
R
Jiang
S
S
Colovai
A
A
Cortesini
R
R
Suciu-Foca
N
N
Harris
P
P
D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology; D018106:CD28 Antigens / Q000096:biosynthesis*; D016827:CD8 Antigens / Q000096:biosynthesis*; D002460:Cell Line; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D005810:Multigene Family / Q000276:immunology; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine; D013556:Swine, Miniature; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*
D015478:Antigens, Heterophile; D018106:CD28 Antigens; D016827:CD8 Antigens; D018984:Epitopes, T-Lymphocyte; D007519:Isoantigens; D016693:Receptors, Antigen, T-Cell, alpha-beta