Response to IL-6 of HPV-18 cervical carcinoma cell lines.
The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls cell type-specific expression of the viral oncoproteins E6 and E7. The HPV-18 URR is active in the cervical carcinoma cell line HeLa but inactive in the hepatoma cell line HepG2. C/EBPss (NF-IL-6) was shown to participate as an important regulator in HPV transcription dependent on the cell type. The finding that C/EPBss is critical for HPV-18 URR activity and that C/EPBss is induced by IL-6 offers the opportunity of manipulating HPV activity by specific cytokine treatment. In this report, we show that treatment with IL-6 results in activation of HPV-18 URR activity in HepG2 cells. In contrast, the HPV-18 URR is not inducible by IL-6 in three cervical carcinoma cell lines. In all three cell lines we found decreased expression of the IL-6 receptor compared to the IL-6-responsive HepG2 cells, whereas the level of expression of the signal transduction component gp130 is present in all cells. These results suggest that cervical carcinoma cells may circumvent the IL-6-induced cellular defense mechanism through downregulation of the IL-6-receptor.
Bauknecht
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T
Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum Heidelberg, Heidelberg, 69120, Germany.
Randelzhofer
B
B
Schmitt
B
B
Ban
Z
Z
Hernando
J J
JJ
D015703:Antigens, CD / Q000235:genetics; D050822:Cytokine Receptor gp130; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology* / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D019947:Receptors, Interleukin-6 / Q000235:genetics; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000821:virology*
D015703:Antigens, CD; C494199:IL6ST protein, human; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130