title: [x811--mutation of the Pseudomonas aeruginosa transposable phage D3112 with a pleiotropic effect].
abstract: Characteristics of Pseudomonas aeruginosa Transposable Phage D3112 carrying mutation x811 are described. x811 is a recessive mutation with pleiotropic effect. It determines a deteriorated lysis of infected or induced bacteria, a delayed replication, and a considerably decreased replication rate. In addition, the x811 mutation is expressed as the Kil phenotype, since high-temperature induction of prophage D3112 cts15 x811 does not cause an immediate decrease in the ability of bacteria to form colonies at 42 degrees C. Restriction analysis of DNA of D3112 cts15 x811 and its segregants has not revealed extended insertions or deletions. The characteristics of segregants of the D3112 cts15 x811 phage agree with the suggestion that the x811 mutation has emerged in a regulatory element (a gene or a site) that controls both expression of the entire early operon, including the "pre-early" function Kil, and the regulation of the repressor synthesis.
authors.affiliation: State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Burkal'tseva
authors.forename: O V
authors.initials: OV
authors.lastname: Shaburova
authors.forename: E A
authors.initials: EA
authors.lastname: Pleteneva
authors.forename: V N
authors.initials: VN
authors.lastname: Krylov
mesh_terms: D004251:DNA Transposable Elements; D005808:Genes, Recessive; D009154:Mutation; D009876:Operon; D010641:Phenotype; D017105:Pseudomonas Phages / Q000235:genetics* / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000821:virology*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014779:Virus Replication / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements
vernacular_title: x811--mutatsiia faga-transpozona D3112 Pseudomonas aeruginosa s pleĭotropnym éffektom.