Gain of Bcl-2 is more potent than bax loss in regulating mammary epithelial cell survival in vivo.
The impact of gain of Bcl-2 function on mammary epithelial cell survival was compared with loss of Bax function during the two stages of mammary gland involution. Bcl-2 gain of function reduced apoptosis 50% during the first stage and increased cell survival 70% during the second stage. Complete loss of Bax reduced apoptosis by 20% during the first stage without second stage effect. Partial loss of Bax was ineffective but increased cell survival 2.4-fold when combined with Bcl-2 gain. Gain of Bcl-2 function is more potent than loss of Bax function in regulating mammary epithelial cell survival in vivo.
Schorr
K
K
Department of Physiology, University of Maryland Medical School, Baltimore 21201, USA.
Li
M
M
Bar-Peled
U
U
Lewis
A
A
Heredia
A
A
Lewis
B
B
Knudson
C M
CM
Korsmeyer
S J
SJ
Jäger
R
R
Weiher
H
H
Furth
P A
PA
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002470:Cell Survival; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein