Effects of oleic acid, docosahexaenoic acid and eicosapentaenoic acid on background and genotoxin-induced frequencies of SCEs in Indian muntjac fibroblasts.
Muntjac cells were cultured at 5 X 10(5) cells/10 cm Petri dish for 24 h prior to addition of fatty acids (50 microM) which were delivered to the cells complexed with 2% bovine serum albumin (fatty acid-free) and incubated for a further 24 h. Parallel dishes were processed for lipid extraction and GC analysis. This analysis showed highly significant (P < 0.01) uptake by the cells of each fatty acid. Genotoxins (75 microM hydrogen peroxide, 20 microM t-butylhydroperoxide and 2.4 microM mitomycin C) were added to the cells for 1 h prior to the end of the 24 h fatty acid incubation period. Control (no genotoxin or fatty acid) treatments were included. No difference was observed in background frequencies of SCEs between controls and fatty acid treatments, thus indicating that these fatty acids per se do not cause DNA damage. The cells incubated with the genotoxins showed increased (P < 0.05) frequencies of SCEs when compared with control frequencies. Cells incubated with genotoxins in the presence of fatty acids also showed significantly higher (P < 0.05) levels of SCEs when compared with control frequencies. When cells supplemented with genotoxins in the presence of fatty acids were compared with cells treated with genotoxins alone, higher levels of SCEs were observed in the former, suggesting that the fatty acids exacerbate DNA damage caused by these genotoxins.
Higgins
S
S
Department of Food Science and Technology, University College, Cork, Republic of Ireland.
Vasconcelos
M H
MH
O'Brien
N M
NM
D000818:Animals; D004249:DNA Damage; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D018668:Muntjacs; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors; D019301:Oleic Acid / Q000494:pharmacology*; D012854:Sister Chromatid Exchange; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D005231:Fatty Acids, Unsaturated; D009153:Mutagens; D019384:Nucleic Acid Synthesis Inhibitors; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D016685:Mitomycin; D020122:tert-Butylhydroperoxide; D015118:Eicosapentaenoic Acid; D006861:Hydrogen Peroxide