title: Antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis patients.
abstract: In the present study, the antibiotic susceptibility of most prevalent micro-organisms in advanced periodontitis patients was evaluated. In 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. From each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (Rapid ID 32 A). From all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for susceptibility testing to tetracycline, metronidazole and amoxicillin/clavulanate using the E Test. The most prevalent microorganisms were Fusobacterium nucleatum (38/214), Peptostreptococcus micros (33/214), Prevotella oralis (33/214) and Porphyromonas gingivalis (32/214). Regarding antibiotic susceptibility it could be shown that minimal inhibitory concentration (MIC) in all cases was below antibiotic concentrations achievable in gingival crevicular fluid. However, antibiotic resistance was seen in 3 to 29% of the investigated microorganisms.
authors.affiliation: Section of Periodontology, College of Dentistry, The Ohio State University, Columbus 43120, USA. kleinfelder.3@osu.edu
authors.forename: J W
authors.initials: JW
authors.lastname: Kleinfelder
authors.forename: R F
authors.initials: RF
authors.lastname: Müller
authors.forename: D E
authors.initials: DE
authors.lastname: Lange
mesh_terms: D000328:Adult; D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects; D003773:Dental Plaque / Q000382:microbiology; D004352:Drug Resistance, Microbial; D005260:Female; D016967:Fusobacterium nucleatum / Q000187:drug effects; D005883:Gingival Crevicular Fluid / Q000382:microbiology; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010462:Peptostreptococcus / Q000187:drug effects; D010512:Periodontal Index; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018720:Prevotella / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole; D019980:Amoxicillin-Potassium Clavulanate Combination; D013752:Tetracycline