Aa-Pri2, a single-copy gene from Agrocybe aegerita, specifically expressed during fruiting initiation, encodes a hydrophobin with a leucine-zipper domain.
The Aa-Pri2 gene, specifically expressed during basidiocarp differentiation of the mushroom Agrocybe aegerita, was cloned. Sequence analysis showed a 525-nt ORF interrupted by three introns (51, 48, 54 bp). One TATA box (-94), two CAAT boxes (-382, -398) and one CT-rich motif (-149) were present. The transcription start point was located by primer extension at position -60. The hydrophobic Aa-PRI2 protein (123 aa) possessed a putative signal peptide (aa1-aa18), eight cysteine residues characteristic of the hydrophobin proteins, and one putative leucine-zipper domain (aa50-aa71). Hybridization of the genomic DNA of several edible mushrooms with the Aa-Pri2 cDNA, showed a 2.7-kb DNA fragment highly similar to the Aa-Pri2 gene in the species Agrocybe chaxingu; sequences showing slight hybridization signals were detected in Pleurotus eryngii, Lentinula edodes and Agaricus bisporus.
Santos
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Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, INRA-Université Victor Segalen Bordeaux 2, Cedex, France.
Labarère
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D000363:Agaricales / Q000235:genetics; D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D017403:In Situ Hybridization; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
C120382:Aa-Pri2 protein, Agrocybe aegerita; D005656:Fungal Proteins