title: A set of plasmids constitutively producing different RNA levels in Escherichia coli.
abstract: New plasmids were developed for the in vivo expression of RNA in Escherichia coli. These plasmids combine constitutive promoters of different strengths with different origins of replication to provide a 75-fold range of expression of amber suppressor tRNA. The plasmids are either pMB1, p15A or temperature-sensitive SC101 replicons, and can be used in two plasmid systems for studying RNA-protein interactions. The temperature-sensitive SC101 plasmids may be useful as gene replacement vectors. Another vector that is suitable for generating lethal mutations was constructed in a plasmid containing a regulatable phage T7 promoter.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, WI, 53706-1567, USA.
authors.forename: K
authors.initials: K
authors.lastname: Gabriel
authors.forename: W H
authors.initials: WH
authors.lastname: McClain
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D005804:Genes, Lethal / Q000235:genetics; D016153:Genes, Suppressor / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000096:biosynthesis* / Q000235:genetics; D012344:RNA, Transfer, Ala / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D012344:RNA, Transfer, Ala; D012313:RNA