The developmental changes of the "paraclaustral reservoir" of migrating cells in the rat brain: a study using morphometric and in situ DNA end labeling techniques.
A distinct group of small cells lying in the ventral part of the external capsule in the rat brain is clearly visible at birth. On the basis of its location (medially to the prepiriform claustrum) and probably its function (as a source of neurons for adjacent structures), we define this nucleus as the "paraclaustral reservoir". The present study reveals the cellular changes of the paraclaustral reservoir during postnatal development of the rat brain using unbiased morphometry and in situ DNA end labeling. During the first 4 days after birth the density and total number of cells in the paraclaustral reservoir were stable; after this period a decrease of these parameters was observed until the complete disappearance of this structure at the end of first postnatal week. The rather low number of TUNEL (TdT mediated dUTP nick end labeling of fragmented DNA) positive nuclei in the paraclaustral reservoir suggests that apoptosis is not a crucial mechanism leading to decay of this structure.
Maciejewska
B
B
Department of Anatomy and Neurobiology, Medical University of Gdańsk, Poland.
Berdel
B
B
Dziewiatkowski
J
J
Moryś
J
J
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D017209:Apoptosis / Q000502:physiology; D001479:Basal Ganglia / Q000166:cytology* / Q000254:growth & development*; D001921:Brain / Q000166:cytology*; D016923:Cell Death / Q000502:physiology; D002465:Cell Movement; D020287:In Situ Nick-End Labeling; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar