Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves.
Four fractions from the leaves of Indigofera oblongifolia were obtained on Sephadex G-25 column chromatography. A single bands of these fractions were detected on Poly-acrylamide SDS gel electrophoresis. An antibacterial action of small protein peptide was tested against Escherichia coli, Klebsiella aerogenes, Kl. pneumoniae. Staphyllococcus aureus, and Bacillus subtilis. Peptide 3 showed strong inhibitory activity against B. subtilis and Aspergillus niger but clear zone of inhibition was also noted against A. fumigatus. Peptide 4 showed significant inhibitory zone against Kl. pneumoniae, S. aureus, B. subtilis. A. fumigatus, A. niger and A. flavus.
Dahot
M U
MU
Department of Biochemistry, Institute of Chemistry, University of Sindh, Jamshoro, Pakistan.
D000890:Anti-Infective Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*
D000890:Anti-Infective Agents; D000935:Antifungal Agents; D010936:Plant Extracts; D011506:Proteins