title: Analysis of loss of heterozygosity in neoplastic nodules induced by diethylnitrosamine in the resistant BFF1 rat strain.
abstract: Loss of heterozygosity (LOH) at specific chromosomal regions is a frequent event in poorly differentiated human hepatocellular carcinomas (HCCs), but rare in mouse HCCs. This behavior could depend on interspecies differences in mechanisms of hepatocarcinogenesis or in developmental stage of lesions. To verify if LOH is involved in rat hepatocarcinogenesis, we studied LOH frequency in slowly growing neoplastic nodules induced by Solt-Farber model in diethylnitrosamine-initiated BFF1 rats. We analyzed, with microsatellites, markers at 67 rat loci dispersed over all chromosomes, corresponding to regions homologous to those lost in human HCCs or containing hepatocellular susceptibility (Hcs) or resistance (Hcr) loci in rat and mouse. In agreement with previous findings with mouse HCCs, but at variance with human HCCs, no detectable LOH was found at any locus in rats, suggesting rare LOH involvement in neoplastic nodules, with low tendency to progress to full malignancy, of BFF1 rats.
authors.affiliation: Department of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gariboldi
authors.forename: R
authors.initials: R
authors.lastname: Pascale
authors.forename: G
authors.initials: G
authors.lastname: Manenti
authors.forename: M R
authors.initials: MR
authors.lastname: De Miglio
authors.forename: D
authors.initials: D
authors.lastname: Calvisi
authors.forename: A
authors.initials: A
authors.lastname: Carru
authors.forename: T A
authors.initials: TA
authors.lastname: Dragani
authors.forename: F
authors.initials: F
authors.lastname: Feo
mesh_terms: D015073:2-Acetylaminofluorene; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D004052:Diethylnitrosamine; D005819:Genetic Markers; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344
chemical_list: D005819:Genetic Markers; D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene