Rapid and simple determination of oxytetracycline in chicken products.
A rapid and simple method for determining residual oxytetracycline (OTC) in chicken products (muscle, liver, and eggs) by high-performance liquid chromatography (HPLC) was developed. Samples were prepared by homogenization with acetonitrile-n-hexane (5 + 4, v/v) followed by centrifugation to minimize fat and protein contents. OTC in the acetonitrile layer was free from interfering compounds when examined by HPLC using a LiChrospher 100 RP-8 end-capped column, a mobile phase of acetonitrile-acetic acid-0.01 M disodium EDTA (28 + 2 + 70, v/v/v), and a photodiode array detector. Average recoveries from samples spiked with OTC (0.1, 0.2, and 1.0 ppm) were > 88%, with coefficients of variation between 2.3 and 5.1%. The limit of detection was 0.05 ppm.
Furusawa
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Osaka City University, Faculty of Human Life Science, Japan.
D000097:Acetonitriles; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002498:Centrifugation; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D010118:Oxytetracycline / Q000032:analysis*; D011202:Poultry Products / Q000032:analysis*; D012680:Sensitivity and Specificity
D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D010118:Oxytetracycline; C032159:acetonitrile