Helicobacter pylori lipopolysaccharide enhances the expression of NADPH oxidase components in cultured guinea pig gastric mucosal cells.
Recently, we showed that cultured guinea pig gastric pit cells possess a phagocyte NADPH oxidase-like activity, which was up-regulated by Helicobacter pylori lipopolysaccharide. We demonstrate here that these cells express all of the phagocyte NADPH oxidase components (gp91-, p22-, p67-, p47-, and p40-phoxes). Treatment with lipopolysaccharide increased the expression of gp91-, p22-, and p67-phoxes, but not that of p47- and p40-phoxes. Intriguingly, the p67-phox expression consistently correlated with up-regulation of superoxide anion-producing ability. Thus, the gastric pit cell NADPH oxidase may play an important role in regulation of the inflammatory response associated with H. pylori infection.
Teshima
S
S
Department of Nutrition, School of Medicine, The University of Tokushima, Tokushima City, Japan.
Tsunawaki
S
S
Rokutan
K
K
D000818:Animals; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006168:Guinea Pigs; D016480:Helicobacter pylori / Q000276:immunology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D019255:NADPH Oxidases / Q000096:biosynthesis* / Q000235:genetics*; D009504:Neutrophils / Q000201:enzymology; D013481:Superoxides / Q000378:metabolism
D008070:Lipopolysaccharides; D013481:Superoxides; D019255:NADPH Oxidases