title: Polypeptide flux through bacterial Hsp70: DnaK cooperates with trigger factor in chaperoning nascent chains.
abstract: A role for DnaK, the major E. coli Hsp70, in chaperoning de novo protein folding has remained elusive. Here we show that under nonstress conditions DnaK transiently associates with a wide variety of nascent and newly synthesized polypeptides, with a preference for chains larger than 30 kDa. Deletion of the nonessential gene encoding trigger factor, a ribosome-associated chaperone, results in a doubling of the fraction of nascent polypeptides interacting with DnaK. Combined deletion of the trigger factor and DnaK genes is lethal under normal growth conditions. These findings indicate important, partially overlapping functions of DnaK and trigger factor in de novo protein folding and explain why the loss of either chaperone can be tolerated by E. coli.
authors.affiliation: Max-Planck-Institut für Biochemie, Department of Cellular Biochemistry, Martinsried, Germany.
authors.forename: S A
authors.initials: SA
authors.lastname: Teter
authors.forename: W A
authors.initials: WA
authors.lastname: Houry
authors.forename: D
authors.initials: D
authors.lastname: Ang
authors.forename: T
authors.initials: T
authors.lastname: Tradler
authors.forename: D
authors.initials: D
authors.lastname: Rockabrand
authors.forename: G
authors.initials: G
authors.lastname: Fischer
authors.forename: P
authors.initials: P
authors.lastname: Blum
authors.forename: C
authors.initials: C
authors.lastname: Georgopoulos
authors.forename: F U
authors.initials: FU
authors.lastname: Hartl
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism; D021983:Cyclophilins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D012270:Ribosomes / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D010455:Peptides; C063842:dnaK protein, E coli; D021983:Cyclophilins; C114522:PPIE protein, human; D019696:Peptidylprolyl Isomerase