title: Effect of growth temperature and vector ends phosphorylation in the cloning efficiency of specific DNA fragments.
authors.affiliation: Veterans Affairs Medical Center, Houston, TX 77030, USA. asepulv@bcm.tmc.edu
authors.forename: A R
authors.initials: AR
authors.lastname: Sepulveda
authors.forename: M W
authors.initials: MW
authors.lastname: Lieberman
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D051379:Mice; D008970:Molecular Weight; D010766:Phosphorylation; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013696:Temperature; D005723:gamma-Glutamyltransferase / Q000235:genetics*
chemical_list: D015342:DNA Probes; D005723:gamma-Glutamyltransferase; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D000469:Alkaline Phosphatase