title: Molecular characterization of Mycobacterium bovis BCG: Romanian sub-strain.
abstract: The Romanian sub-strain used for BCG vaccine production was characterized by polymorphic GC-rich repetitive sequence (PGRS) restriction profile and IS6110 detection. For comparison, the Pasteur and Moscow Mycobacterium bovis BCG sub-strains, M. bovis AN5, and M. tuberculosis H37Rv were analyzed. The BCG sub-strains showed the same restriction profile for PGRS after Pvu II, BamH I and Sal I digestion. They could be distinguished from the strains M. bovis AN5 and M. tuberculosis H37Rv after Pvu II and Sal I digestion. Sal I can also distinguish between M. bovis AN5 and M. tuberculosis H37Rv. Digestion with Pvu II, Sal I, Sca I, Mlu I and BamH I gave identical profiles of IS6110 hybridization for the Romanian and Pasteur BCG substrains.
authors.affiliation: Cantacuzino Institute, Bucharest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Balazs
authors.forename: R
authors.initials: R
authors.lastname: Cojocaru
authors.forename: M
authors.initials: M
authors.lastname: Damian
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000145:classification* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial