Induction of matrix metalloprotease-1 gene expression by retinoic acid in the human pancreatic tumour cell line Dan-G.
We have investigated the effects of retinoic acid (RA) on matrix metalloprotease-1 (MMP-1) gene expression in the human pancreatic tumour cell line Dan-G. 13-cis RA results in a time- and dose-dependent increase of MMP-1 protein concentration. These stimulatory effects were paralleled by a time- and dose-dependent increase of MMP-1 mRNA steady-state concentrations. Nuclear run-on analysis revealed that the increase of MMP-1 mRNA was partially due to an increase of MMP-1 gene transcription. In addition, 13-cis RA treatment results in an increase of MMP-1 mRNA stability. These data demonstrate that RA stimulates MMP-1 gene expression in human pancreatic carcinoma cells by transcriptional and post-transcriptional mechanisms.
von Marschall
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Z
Medizinische Klinik I, Gastroenterologie/Infektiologie, Klinikum Benjamin Franklin, Berlin, Germany.
Riecken
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EO
Rosewicz
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D015152:Blotting, Northern; D015153:Blotting, Western; D017364:Collagenases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D015474:Isotretinoin / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1; D010190:Pancreatic Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D017364:Collagenases; D020781:Matrix Metalloproteinase 1; D015474:Isotretinoin