title: Detection of low levels of flunitrazepam and its metabolites in blood and bloodstains.
abstract: Detection of low levels of flunitrazepam and its metabolites was developed using solid-phase extraction to isolate the drugs from whole liquid blood and dried bloodstains, with subsequent derivatization with pentafluoropropionic anhydride (PFPA) followed by N-(tert-butyldimethyl-silyl)-N-methyl-trifluoroacetamide (MTBSTFA) with 1% TBDMSCI. Analysis was confirmed by gas chromatography/mass spectroscopy using select ion monitoring (sim) in electron impact mode. The limit of detection of this procedure using 1 ml of blood was determined to be 0.1 microgram/dl.
authors.affiliation: Commonwealth of Massachusetts, Forensic Toxicology Laboratory, Sudbury 01776, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Elian
mesh_terms: D014151:Anti-Anxiety Agents / Q000097:blood* / Q000378:metabolism; D001801:Blood Stains; D005445:Flunitrazepam / Q000097:blood* / Q000378:metabolism; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
chemical_list: D014151:Anti-Anxiety Agents; D005445:Flunitrazepam