Poly(A) mRNA is attached to insect ovarian microtubules in vivo in a nucleotide-sensitive manner.
In ovarioles of hemipteran insects, RNA passes from anteriorly positioned nurse cells to the chain of developing oocytes via extended nutritive tubes. These intercellular connections may reach several millimeters in length. Each nutritive tube is comprised of many thousands of parallel microtubules. We have extracted microtubule bundles from isolated nutritive tubes of Notonecta glauca and, using hybridization techniques, provide evidence of poly(A) mRNA attachment to microtubules in vivo. We also show this attachment to be nucleotide-sensitive, which is typical of a motor protein-mediated interaction. The pattern of nucleotide sensistivity is indicative of a kinesin motor mechanism. We provide evidence that a kinesin is present in the nutritive tube translocation channels and is a component of the mRNA/microtubule bundles isolated and extracted from them. Our findings are consistent with kinesin-driven transport of mRNA along the nutritive tube microtubules.
Stephen
S
S
School of Biological Sciences, Washington Singer Laboratories, University of Exeter, United Kingdom.
Talbot
N J
NJ
Stebbings
H
H
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005260:Female; D006160:Guanosine Triphosphate / Q000378:metabolism; D006430:Hemiptera; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
D000227:Adenine Nucleotides; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); C030570:oligodeoxyadenylic acid; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D016547:Kinesins