title: Degradation of Japanese encephalitis virus by neutrophils.
abstract: The ability of neutrophils to degrade the phagocytosed Japanese encephalitis (JE) virion, via triggering of the respiratory burst and generation of toxic radicals has been investigated. JEV or JEV-induced macrophage derived factor (MDF) induces increase in intracellular oxidative signals with generation of superoxide anion (O2-), via activation of cytosolic NADPH and subsequent formation of hydrogen peroxide, with maximum activity on day 7 post infection. The response was sensitive to anti-MDF antibody treatment. Further, the study revealed rapid degradation of phagocytosed JE viral protein and nucleic acid. The viral protein degradation was partially dependent on the generation of toxic oxygen species as it could be abrogated by pretreatment of the cells with staurosporine.
authors.affiliation: Postgraduate Department of Microbiology, King George's Medical College, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Srivastava
authors.forename: N
authors.initials: N
authors.lastname: Khanna
authors.forename: S K
authors.initials: SK
authors.lastname: Saxena
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.forename: A
authors.initials: A
authors.lastname: Mathur
authors.forename: T N
authors.initials: TN
authors.lastname: Dhole
mesh_terms: D000818:Animals; D002630:Chemotactic Factors / Q000494:pharmacology; D004672:Encephalitis, Japanese / Q000276:immunology*; D005416:Flavivirus / Q000276:immunology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis; D016897:Respiratory Burst; D014771:Virion / Q000276:immunology
chemical_list: D002630:Chemotactic Factors; D006861:Hydrogen Peroxide; D009195:Peroxidase; D019255:NADPH Oxidases