title: [Investigation on the marker substances of crude drugs in formulations. I. Marker substances for the identification of astragali radix in kampo and drinkable preparations].
abstract: There are few reports about marker substances for the identification of Astragali Radix in formulations. First, constituents analysis was performed by HPLC for the screening of a marker substance, using several lots of Astragali Radix and its fluid extracts. As a result of the analysis, one of the main components was clearly detected even in the fluid extracts, and thought to be a good marker substance. This component was identified to be calycosin (7,3'-dihydroxy-4'-methoxyisoflavone) by the structural analysis. Then, the identification methods of Astragali Radix and its fluid extracts in formulations were investigated with an Astragali Radix extract as a reference standard solution and calycosin as a marker substance. This method was applied to three different kinds of Kampo formulations and three different kinds of drinkable preparations. Consequently, calycosin was clearly detected by a HPLC-multi wavelength detector system, in all investigated formulations. Calycosin will be successfully used as a marker substance for the identification of Astragali Radix and its fluid extracts in formulations.
authors.affiliation: Analytical Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Nishi
authors.forename: Y
authors.initials: Y
authors.lastname: Kokusenya
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007529:Isoflavones / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C121707:7,3'-dihydroxy-4'-methoxyisoflavone