Identification of Staphylococcus carnosus and Staphylococcus warneri isolated from meat by fluorescent in situ hybridization with 16S rRNA-targeted oligonucleotide probes.
16S rRNA targeted oligonucleotide probes were designed by sequence analysis of an rRNA database to discriminate S. carnosus, S. warneri, and S. saprophyticus species. After establishing hybridization conditions by RNA dot blot hybridization with reference species, our probes were shown to be specific. By in situ hybridization only S-S-S.carno-0440-a-A-23 and S-S-S.war-0180-a-A-23 can specifically detect S. carnosus and S. warneri, respectively. The detection of old cells of S. carnosus 833 was more limited by the permeabilisation than by the low rRNA content. One day old cells could be permeabilized with lysostaphin, whereas young cells were permeabilized with lysozyme.
Gory
L
L
Station de recherces sur la viande, INRA, Clermont-Theix, Saint-Genès Champanelle.
Millet
L
L
Godon
J J
JJ
Montel
M C
MC
D001483:Base Sequence; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S