title: Phenol degradation by an enterobacterium: a Klebsiella strain carries a TOL-like plasmid and a gene encoding a novel phenol hydroxylase.
abstract: Although phenol catabolism is described for many different microorganisms, there is no example for such a pathway in an enterobacterial strain. Here we characterize a Klebsiella oxytoca strain that grows on phenol as the only source of carbon and energy. As the key enzyme of phenol degradation, phenol hydroxylase was purified to apparent homogeneity. Compared with other phenol hydroxylases, the Klebsiella enzyme differs with respect to several properties: (i) SDS-PAGE and gel-filtration analysis of the purified protein revealed that the enzyme is a monomer with a molecular mass of 156 kDa; (ii) steady-state kinetic measurements resulted in a K(m) value of 0.22 mM for phenol; and (iii) the enzyme is both dependent on NADPH/FAD and sensitive to EDTA. Further degradation of catechol, the reaction product of phenol hydroxylase, may occur via the effective meta-fission pathway often located on TOL or TOL-like plasmids. Such a plasmid was prepared from the Klebsiella strain and further characterized. The given data demonstrate that the isolated strain exhibits all characteristics of an efficient phenol-degrading microorganism.
authors.affiliation: Thgymoorgan GmbH, Vienenburg, Germany. kerstinh@uni-wh.de
authors.forename: K
authors.initials: K
authors.lastname: Heesche-Wagner
authors.forename: T
authors.initials: T
authors.lastname: Schwarz
authors.forename: M
authors.initials: M
authors.lastname: Kaufmann
mesh_terms: D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D002396:Catechols / Q000378:metabolism; D049308:Dioxygenases; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007709:Klebsiella / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010105:Oxygenases / Q000032:analysis; D019800:Phenol / Q000378:metabolism*; D010957:Plasmids / Q000032:analysis / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D002396:Catechols; D019800:Phenol; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; C022336:phenol 2-monooxygenase; C034221:catechol