Inhibitory effect of trapidil on proliferation of cultured rat aortic smooth muscle cells induced by endothelin-1.
OBJECTIVE
To study the effect of trapidil (Tra) on endothelin-1-induced proliferation of cultured rat aortic vascular smooth muscle cells (VSMC).


METHODS
The [3H]TdR incorporation into DNA assay, the number of VSMC, and cell cycle distribution were measured.


RESULTS
Pretreated with endothelin-1 100 nmol.L-1, cell number, [3H]TdR uptake, and cell mitogenic activity increased 134% +/- 23%, 210% +/- 70%, and 86% +/- 18%, respectively. This proliferation was inhibited by Tra 5, 50, 500 mumol.L-1. The inhibitory rates were 12%-48%, 35%-54% and 15%-47%, respectively. Tra did not influence the proliferation of VSMC without endothelin-1 pretreatment.


CONCLUSIONS
Tra antagonized the proliferation of VSMC induced by endothelin-1.
Cheng
Y
Y
Institute of Clinical Pharmacology, Tongji Medical University, Wuhan, China. chenyan@public.wh.hb.cn
Gong
P L
PL
Zeng
F D
FD
Liu
X H
XH
Su
J Y
JY
Tang
C S
CS
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000037:antagonists & inhibitors*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D014192:Trapidil / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
D019332:Endothelin-1; D014665:Vasodilator Agents; D014192:Trapidil