Engraftment and multilineage expression of human bone marrow CD34- cells in vivo.
The fetal sheep competitive engraftment model of human hematopoietic stem cells (HSC) was used to evaluate the in vivo engraftment potential of human bone marrow CD34- Lin- cells. Transplantation of CD34- Lin- cells into primary hosts resulted in the long-term (> 1 year) engraftment and multilineage donor cell/progenitor expression with production of significant numbers of CD34+ cells. Secondary transplantation and limiting dilution studies confirmed the presence in human CD34- fraction of HSC with in vivo long-term engraftment and multilineage differentiation potentials.
Zanjani
E D
ED
Department of Veterans Affairs Medical Center, University of Nevada Reno 89520, USA. zanjani@scs.unr.edu
Almeida-Porada
G
G
Livingston
A G
AG
Porada
C D
CD
Ogawa
M
M
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D002478:Cells, Cultured; D005333:Fetus; D006085:Graft Survival / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D012756:Sheep; D014183:Transplantation, Heterologous / Q000276:immunology / Q000502:physiology*
D015703:Antigens, CD; D018952:Antigens, CD34; D015415:Biomarkers