title: Electrochemotherapy against colorectal carcinoma: comparison of in vitro cytotoxicity of 5-fluorouracil, cisplatin and bleomycin.
abstract: Effectiveness of electrochemotherapy against colorectal carcinoma (CRC) was investigated in vitro using murine CRC cell lines. Electropermeabilization did not increase the sensitivity of CRC cells to 5-fluorouracil or cisplatin. Conversely, electropermeabilization markedly increased the sensitivity of CRC cells to bleomycin (BLM), resulting in 1,000-fold higher susceptibility. Subsequent analyses revealed that electropermeabilization significantly increased intracellular BLM levels of CRC cells. These results suggest that electrochemotherapy with BLM is a promising modality for the treatment of CRC, and that electrochemotherapy can be translated from the treatment of superficial tumors to the treatment of internal tumors including CRC.
authors.affiliation: Third Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8522, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kuriyama
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Mitoro
authors.forename: H
authors.initials: H
authors.lastname: Tsujinoue
authors.forename: Y
authors.initials: Y
authors.lastname: Toyokawa
authors.forename: T
authors.initials: T
authors.lastname: Nakatani
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
authors.forename: S
authors.initials: S
authors.lastname: Okamoto
authors.forename: H
authors.initials: H
authors.lastname: Fukui
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000188:drug therapy* / Q000453:epidemiology / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D001761:Bleomycin / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D002463:Cell Membrane Permeability; D002945:Cisplatin / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000453:epidemiology / Q000473:pathology; D016895:Culture Media, Serum-Free; D004127:Dimethylhydrazines; D004354:Drug Screening Assays, Antitumor; D018274:Electroporation; D005472:Fluorouracil / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D016895:Culture Media, Serum-Free; D004127:Dimethylhydrazines; D001761:Bleomycin; D002945:Cisplatin; D005472:Fluorouracil