title: Antioxidant enzymes in the macular retinal pigment epithelium of eyes with neovascular age-related macular degeneration.
abstract: OBJECTIVE
  To test the hypothesis that neovascular age-related macular degeneration is related to oxidative stress involving the macular retinal pigment epithelium. This study investigated, as a function of age, levels of enzymes that defend tissues against oxidative stress in the macular retinal pigment epithelium of human eyes with this disease.
  
  
  METHODS
  Surgical specimens of macular choroidal neovascular membranes from eyes with age-related macular degeneration and the macular regions of whole donor eyes with neovascular age-related macular degeneration or without evident ocular disease were studied by quantitative electron microscopic immunocytochemistry with colloidal gold-labeled second antibodies. Relative levels in retinal pigment epithelium cell cytoplasm and lysosomes were determined of five enzymes believed to protect cells from oxidative stress, as well as levels of the retinal pigment epithelium marker cytoplasmic retinaldehyde-binding protein, for comparison with the enzymes.
  
  
  RESULTS
  Copper, zinc superoxide dismutase immunoreactivity increased and catalase immunoreactivity decreased with age in cytoplasm and lysosomes from macular retinal pigment epithelium cells of normal eyes and eyes with age-related macular degeneration. Cytoplasmic retinaldehyde-binding protein immunoreactivity showed no significant relationship to age or the presence of neovascular age-related macular degeneration. Glutathione peroxidase immunoreactivity was absent from human retinal pigment epithelium cells. Both heme oxygenase-1 and heme oxygenase-2 had highly significantly greater immunoreactivity in retinal pigment epithelium cell lysosomes than in cytoplasm, differing from the much greater cytoplasmic immunoreactivity of the other proteins studied. This immunoreactivity decreased with age, particularly in the lysosomes of retinal pigment epithelium cells from eyes with neovascular age-related macular degeneration. These decreases were of borderline significance (P = .067 for heme oxygenase-1; P = .12 for heme oxygenase-2) when eyes with age-related macular degeneration were compared with normal eyes by multivariable logistic regression.
  
  
  CONCLUSIONS
  The high heme oxygenase-1 and heme oxygenase-2 lysosomal antigen levels in macular retinal pigment epithelium cells of eyes with neovascular age-related macular degeneration suggest that oxidative stress causes a pathologic upregulation of these enzymes. Increased lysosomal disposal may indicate that the reparative functions of these enzymes are accompanied by deleterious effects, necessitating their rapid removal from the cell. The much higher heme oxygenase-1 and heme oxygenase-2 antigen levels in macular retinal pigment epithelium cells from younger individuals suggest that protective mechanisms against oxidation and, hence, presumably to the development of age-related macular degeneration, decrease with age.
authors.affiliation: Kresge Eye Institute of Wayne State University School of Medicine, Detroit, Michigan 48201, USA. rnfrank@med.wayne.edu
authors.forename: R N
authors.initials: RN
authors.lastname: Frank
authors.forename: R H
authors.initials: RH
authors.lastname: Amin
authors.forename: J E
authors.initials: JE
authors.lastname: Puklin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D020256:Choroidal Neovascularization / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D051547:Heme Oxygenase-1; D006801:Humans; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008266:Macula Lutea / Q000201:enzymology* / Q000648:ultrastructure; D008268:Macular Degeneration / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000201:enzymology* / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C020932:11-cis-retinal-binding protein; D000975:Antioxidants; D002352:Carrier Proteins; D008565:Membrane Proteins; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D013482:Superoxide Dismutase