title: Revelation of Antiviral Activities by Artificial Sulfation of a Glycosaminoglycan from a Marine Pseudomonas.
abstract: : Sulfated derivatives of a glycosaminoglycan containing l-glutamic acid produced by a marine Pseudomonas species, No. 42 strain, were prepared by the method of dicyclohexyl-carbodiimide-mediated reaction. Both low and high degrees of sulfation of the polysaccharides (products A1 and A2, respectively) were investigated for their antiviral activities against influenza virus type A (FluV-A) and B (FluV-B) in MDCK cells. Both preparations showed antiviral activity against FluV-A at the 50% antiviral effective concentration of 17.3 and 5.2 µg/ml, respectively, whereas they had no antiviral activity against FluV-B. No cytotoxicity of either product was noted against MDCK cells at the 50% cytotoxic concentration of 100 µg/ml.
authors.affiliation: Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan
authors.initials: AS
authors.lastname: Ahmad
authors.initials: M
authors.lastname: Matsuda
authors.initials: S
authors.lastname: Shigeta
authors.initials: K
authors.lastname: Okutani