Real-time monitoring of enzymatic cleavage of nucleic acids using a quartz crystal microbalance.
The use of quartz crystal microbalance (QCM) for monitoring in situ the enzymatic cleavage of surface-confined nucleic acids by nucleases is described. Such real-time monitoring of mass changes associated with the enzymatic digestion indicates that the activity and specificity of nucleases is preserved at the gold surface, and can be used for manipulating surface-confined DNAs and RNAs. These observations indicate great promise for using QCM for elucidating the interactions of nucleic acids with enzymes, and for enhancing the power of hybridization biosensors.
Wang
J
J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA. joewang@nmsu.edu
Jiang
M
M
Palecek
E
E
D003460:Crystallization; D003851:Deoxyribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D009696:Nucleic Acids / Q000378:metabolism*; D011791:Quartz; D012260:Ribonucleases / Q000378:metabolism*
D015341:Nucleic Acid Probes; D009696:Nucleic Acids; D011791:Quartz; D003851:Deoxyribonucleases; D012260:Ribonucleases