title: The role of the alternative respiratory pathway in the stimulation of cephalosporin C formation by soybean oil in Acremonium chrysogenum.
abstract: Addition of soybean oil to Acremonium chrysogenum cultures growing on sugars doubled the specific production of cephalosporin C during the idiophase of growth. While the addition of soybean oil had no effect on the total rate of respiration, the respiration that proceeded via the alternative, cyanide-insensitive pathway exhibited a more than twofold increase. Addition of soybean oil also stimulated the formation of isocitrate lyase activities. Inhibition of oxidative metabolism of one of the products of isocitrate lyase (succinate) by thenoyltrifluoroacetone completely inhibited the alternative respiratory pathway. The role of soybean-oil-stimulated alternative respiration in the stimulation of cephalosporin C production and the role of isocitrate lyase are discussed.
authors.affiliation: Department of Microbiology and Biotechnology, Kossuth Lajos University, Debrecen, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Karaffa
authors.forename: E
authors.initials: E
authors.lastname: Sándor
authors.forename: J
authors.initials: J
authors.lastname: Kozma
authors.forename: C P
authors.initials: CP
authors.lastname: Kubicek
authors.forename: A
authors.initials: A
authors.lastname: Szentirmai
mesh_terms: D000164:Acremonium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002511:Cephalosporins / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation / Q000187:drug effects / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D007522:Isocitrate Lyase / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D013024:Soybean Oil / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors; D019802:Succinic Acid / Q000378:metabolism; D013804:Thenoyltrifluoroacetone / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002511:Cephalosporins; D003470:Culture Media; D013804:Thenoyltrifluoroacetone; C025163:cephalosporin C; D013024:Soybean Oil; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; D007522:Isocitrate Lyase; D005947:Glucose