[Genotyping of HCV isolates from different populations in Shanghai by using second generation line probe assay].
OBJECTIVE
To investigate the distribution and frequency of various genotypes in different populations in Shanghai.


METHODS
109 HCV isolates by using RT-nested PCR including 81 from patients with hepatitis C (PHCs), 7 from blood donors (BDs) and 21 from intravenous drug abusers (IVDAs) were genotyped by using recently improved second generation line probe assay (INNO-LIPA HCV II innogenetics N.V.Belgium).


RESULTS
Among 81 HCV isolates from PHCs, there were 71 (87.6%) genotype 1b, 4(4.9%)2a, 2(2.5%)3b, 1(1.2%)6a, 2(2.5%) mixed genotypes (1 for 2a or 2c + 2b + 1, 1 for 1b + 6a) and 1 (1.2%) undeterminable respectively, and among 7 from BDs, there were 5 1b and 2 genotype 2a. But among 21 from IVDAs, there were 8(38.1%) genotype 1a, 5(23.8%)1b, 2(9.5%)2a or 2c, 2(9.5%)3a, 1(4.8%) 3b and 3(14.3%) mixed genotypes (1 for 1a + 2a or 2c, 1 for 1b + 2a or 2c + 6a and 1 for 1a + 1b + 3b) respectively.


CONCLUSIONS
(1) 7 HCV subtypes (1a, 1b, 2a, 2b, 3a, 3b and 6a) belonging to 4 HCV genotypes (1, 2, 3, 6) were present in different populations in Shanghai. Of them, 2b, 3a, 3b and 6a were first found. (2) HCV genotype 1b was most commonly found in PHCs and BDs. 1a and 3a were only found in IVDAs. (3) The fact that more HCV subtypes were found in IVDAs in this city.
Jiang
W
W
Shanghai Municipal Infectious Disease Hospital.
Gu
S
S
Hu
Y
Y
D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged