An association between LSIL and the high secretor phenotype of IL-1beta.
OBJECTIVE
The aims of this study were to determine the frequency of a nucleotide transition from C to T, which leads to increased transcription of interleukin-1beta (IL-1beta) in patients with different grades of cervical lesions, and to determine whether a correlation exists between the genotypes and cervical lesions.


METHODS
One hundred forty-seven DNA samples from patients with different grades of cervical lesions were compared with 100 healthy, age and sex-matched bone marrow donors. TaqI restriction digest of PCR products was used to analyze the IL-1beta +5887 C --> T mutation and the results were confirmed using induced heteroduplex analysis with an induced heteroduplex generator.


RESULTS
The Pearson chi2 test (Yate's correction) was used for statistical analysis. Patients with LSIL (n = 80) demonstrated a high frequency of allele T, previously associated with high IL-1beta secretor phenotype, compared to controls (P = 0.000012). A trend was also observed in patients with HSIL (n = 28, P = 0.039).


CONCLUSIONS
We report for the first time a highly significant association between high secretor IL-1beta phenotypes (i.e., IL-1beta +5887 CT or TT genotypes) and LSIL. A less significant association exists with HSIL. The intrinsic ability to produce variable amounts of IL-1beta during different clinical stages involving cervical lesions may be of immunological importance in their pathogenesis.
Majeed
G S
GS
Department of Obstetrics & Gynaecology and Pathology & Microbiology, University of Bristol, Bristol, BS2 8EG, United Kingdom.
Glew
S
S
Bidwell
J
J
D000230:Adenocarcinoma / Q000235:genetics*; D002278:Carcinoma in Situ / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003596:Cytosine / Q000032:analysis; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D013941:Thymine / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D007375:Interleukin-1; D003596:Cytosine; D013941:Thymine