[Malonyldialdehyde and total antioxidant status in the peritoneal fluid of infertile women].
OBJECTIVE
To estimate the concentration of malonyldialdehyde (MDA) and total antioxidant status in the peritoneal fluid (PF) of patients with unexplained infertility (UI) and infertile women with minimal and mild endometriosis.


METHODS
PF was obtained during laparoscopy from 8 women with UI, 12 infertile women with endometriosis (I degree and II degrees rAFS) and 10 women with benign noninflammatory ovarian tumours. All laparoscopies were performed in the follicular phase of the cycle. MDA concentration was measured according to Ledwozyw method, TAS was measured spectrophotometrically using RANDOX diagnostic reagent system.


RESULTS
We found significantly higher concentration of MDA in PF from both patients with UI (p = 0.03) and with endometriosis (p = 0.046) compared to the control group. TAS was significantly (p = 0.027) higher in PF of women with UI but did not differ significantly (p = 0.49) between patients with endometriosis and controls.


CONCLUSIONS
Our results show that an imbalance between lipid peroxides and the antioxidant system in PF environment may be one of the main factors responsible for the UI. In the group with endometriosis a marginally significant difference in MDA levels, no significant differences in TAS and data from the literature, suggest that accelerated lipid peroxidation in PF doesn't appear to play a role in the endometriosis associated infertility.
Polak
G
G
Katedry i Zakładu Mikrobiologii Lekarskiej AM w Lublinie.
Rola
R
R
Gogacz
M
M
Kozioł-Montewka
M
M
Kotarski
J
J
D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001202:Ascitic Fluid / Q000737:chemistry*; D004715:Endometriosis / Q000150:complications; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000150:complications / Q000175:diagnosis*; D007813:Laparotomy / Q000379:methods; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000150:complications
D000975:Antioxidants; D008315:Malondialdehyde
Stezenie dialdehydu malonowego oraz całkowity potencjał antyoksydacyjny w płynie otrzewnowym niepłodnych kobiet.