Molecular basis of feline beta-glucuronidase deficiency: an animal model of mucopolysaccharidosis VII.
A family of domestic cats was found that exhibited clinical and biochemical abnormalities consistent with mucopolysaccharidosis VII, an autosomal recessive lysosomal storage disorder caused by beta-glucuronidase deficiency. beta-Glucuronidase activity was undetectable in affected cat fibroblasts and restored by retroviral gene transfer of rat beta-glucuronidase cDNA. beta-Glucuronidase mRNA was normal in affected cat testis by Northern blot analysis. Normal feline beta-glucuronidase cDNA was cloned and characterized, and amplified from affected cat fibroblasts by reverse transcription coupled polymerase chain reaction. There was a G-to-A transition in the affected cat cDNA that predicted an E351K substitution, destroyed a BssSI site, and eliminated GUSB enzymatic activity in expression studies. Multiple species comparison and the crystal structure of human beta-glucuronidase indicated that E351 is a highly conserved residue most likely essential in maintenance of the enzyme's conformation. BssSI digestion of polymerase chain reaction products amplified from genomic DNA indicated that affected cats were homozygous and cats with half-normal beta-glucuronidase activity were heterozygous for the missense mutation. Carriers identified in this manner produced affected kittens in prospective breedings, and a feline MPS VII breeding colony has been established.
Fyfe
J C
JC
College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA. fyfe@cvm.msu.edu
Kurzhals
R L
RL
Lassaline
M E
ME
Henthorn
P S
PS
Alur
P R
PR
Wang
P
P
Wolfe
J H
JH
Giger
U
U
Haskins
M E
ME
Patterson
D F
DF
Sun
H
H
Jain
S
S
Yuhki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D004252:DNA Mutational Analysis; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D005966:Glucuronidase / Q000172:deficiency*; D006025:Glycosaminoglycans / Q000652:urine; D006801:Humans; D008297:Male; D008956:Models, Chemical; D008969:Molecular Sequence Data; D016538:Mucopolysaccharidosis VII / Q000235:genetics*; D010375:Pedigree; D010641:Phenotype; D051381:Rats; D017386:Sequence Homology, Amino Acid
D006025:Glycosaminoglycans; D005966:Glucuronidase