The application of comparative genomic hybridization and fluorescence in situ hybridization to the characterization of genotoxicity screening tester strains AHH-1 and MCL-5.
AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasmids. The metabolic components of these cell lines make them a useful screening tool for use in mutagenicity studies. Although AHH-1 and MCL-5 are closely related, the two cell lines show differences which cannot be attributed to transfection. In the present study both cell lines were investigated for chromosome stability by comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) using whole chromosome probes and telomeric probes. Amplification in chromosomes 4q, 3q and 9p was observed in both cell lines. To compare the cell lines directly, AHH-1 and MCL-5 DNAs were co-hybridized on the same metaphases using a modified CGH technique. The only difference observed between AHH-1 and MCL-5 was the degree of amplification involving the subtelomeric region of chromosome 4; the additional telomeric region (4q) was translocated onto chromosome 11 and/or chromosome X. FISH was use to show the presence of isochromosomes 3q and 9p in both cell lines with a chromosome number of 48 or higher. These data demonstrate that CGH and FISH with chromosome-specific probes are able to resolve complex karyotypes and to highlight subchromosomal regions involved in rearrangements and potential chromosome fragile sites. Analyses such as those described here may be of considerable value in the determination of the stability of a variety of the cell lines used in the mutagenicity testing of chemicals.
Corso
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Center for Molecular Genetics and Toxicology, School of Biological Sciences, University of Wales-Swansea, Swansea SA2 8PP, UK. bacorso@swansea.ac.uk
Parry
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D001711:Biotransformation; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics; D020223:Chromosome Painting / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism; D015342:DNA Probes; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods
D015342:DNA Probes; D003577:Cytochrome P-450 Enzyme System