[Aerosolized recombinant interferon-gamma prevent antigen-induced eosinophil recruitment in guinea pig trachea].
OBJECTIVE
In order to determine whether interferon-gamma (IFN-gamma) inhibits eosinphil infiltration in the trachea of asthmatic guinea pigs induced by Rhizopus nigricans.


METHODS
We had administered aerosolized rIFN-gamma in the tracheas of 30 sensitized guinea pigs which had been divided into six groups, then teated animal inhaled rIFN-gamma of 5 x 10(4), 20 x 10(4), and 40 x 10(4) concentration, BDP and normal saline respectively at 24 h, 12 h, 2 h before being challenged.


RESULTS
(1) Provocation positive rates decreased in 40 x 10(4) rIFN-gamma and BDP group compared with that in normal saline group and before intervention (P < 0.05), airway resistence decreased (P < 0.01). (2) The administration of aerosolized rIFN-gamma (40 x 10(4)) and BDP also decreased fungus-induced eosnophils but not other cells infiltration in the trachea. (3) In BALF, Eos count and ECP level were obviously lower than those in other groups. However, eosinophil numbers did not show significant change in the peripheral blood.


CONCLUSIONS
Local administration of rIFN-gamma (40 x 10(4)) may reduce airway inflammation and intervene asthmatic attack by inhibition of Eos, ECP infiltration in airways.
Gao
Y
Y
Respiratory Department, General Hospital of PLA Shenyang.
Chenping
Lin
X P
XP
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001249:Asthma / Q000209:etiology / Q000473:pathology*; D001798:Blood Proteins / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000473:pathology*; D006168:Guinea Pigs; D007371:Interferon-gamma / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D011994:Recombinant Proteins; D012233:Rhizopus; D012260:Ribonucleases
D000336:Aerosols; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; D012260:Ribonucleases