title: Relationship between radical intensity and biological activity of cacao husk extracts.
abstract: The relationship between radical intensity and biological activity of cacao husk extracts was investigated. Electron spin resonance (ESR) spectroscopy demonstrated that the radical intensity of hexane, acetone, methanol and 70% methanol extracts increased with water-solubility. Several fractions of these husk extracts, separated by different column chromatographies, significantly inhibited the cytopathic effect of human immunodeficiency virus (HIV) infection in parallel with their radical intensity. However, their cytotoxic activity against human leukemic and carcinoma cell lines is not always correlated with their radical intensity. Water-soluble and lipophilic compounds might induce cytotoxic activity by different mechanisms.
authors.affiliation: Meiji Pharmaceutical University, Tokyo, Japan. motohasi@my-pharm.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Motohashi
authors.forename: M
authors.initials: M
authors.lastname: Kawase
authors.forename: T
authors.initials: T
authors.lastname: Kurihara
authors.forename: Y
authors.initials: Y
authors.lastname: Shirataki
authors.forename: K
authors.initials: K
authors.lastname: Kamata
authors.forename: H
authors.initials: H
authors.lastname: Nakashima
authors.forename: M
authors.initials: M
authors.lastname: Premanathan
authors.forename: R
authors.initials: R
authors.lastname: Arakaki
authors.forename: K
authors.initials: K
authors.lastname: Kanbara
authors.forename: K
authors.initials: K
authors.lastname: Satoh
authors.forename: H
authors.initials: H
authors.lastname: Sakagami
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology; D002099:Cacao; D005609:Free Radicals; D018922:HL-60 Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012995:Solubility
chemical_list: D019380:Anti-HIV Agents; D005609:Free Radicals; D010936:Plant Extracts