title: NILR-1, a novel immunoglobulin-like receptor expressed by neutrophilic granulocytes, is encoded by a leukocyte receptor gene complex on rat chromosome 1.
abstract: Several receptors expressed by subsets of leukocytes and with sequence homology to the killer cell inhibitory receptors have recently been identified both in man and mouse. Here we describe a rat cDNA that encodes a novel receptor of this group, designated neutrophil immunoglobulin-like receptor-1 (NILR-1). The predicted 58.7-kDa mature NILR-1 protein is a type I integral membrane protein, with three C2-type immunoglobulin superfamily domains, a transmembrane region devoid of charged amino acids, and a cytoplasmic tail containing four immunoreceptor tyrosine-based inhibition motif-like regions. NILR-1 shows greatest sequence homology to the mouse paired immunoglobulin-like receptor-B and members of the human leukocyte immunoglobulin-like receptor/immunoglobulin-like transcript group of receptors. As shown by Northern blot analysis, NILR-1 was transcribed by neutrophilic granulocytes. Although weaker transcription was found with a macrophage cell line, no signal was detected with peritoneal macrophage or spleen RNA. Linkage analysis localized Nilr1 to chromosome 1, closely linked to a locus encoding a rat NKp46 orthologue. The two loci define a rat leukocyte receptor gene complex, in a region syntenic to human chromosome 19q13.4 and the proximal part of mouse chromosome 7, that harbors the human and mouse leukocyte receptor gene complexes.
authors.affiliation: Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Norway. s.f.berg@basalmed.uio.no
authors.forename: S F
authors.initials: SF
authors.lastname: Berg
authors.forename: S
authors.initials: S
authors.lastname: Fossum
authors.forename: E
authors.initials: E
authors.lastname: Dissen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D007136:Immunoglobulins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; C119832:neutrophil immunoglobulin-like receptor 1