title: Absence of antisperm surface antibodies in prepubertal boys with cryptorchidism and other anomalies of the inguinoscrotal region before and after surgery.
abstract: OBJECTIVE
  Although the prepubertal immune system cannot recognize postmeiotic germ cell antigens, an overall 21 to 28% incidence of antisperm antibodies directed at these antigens has been reported preoperatively in prepubertal children with cryptorchidism and other inguinoscrotal anomalies. We investigated the prevalence of antisperm antibodies in these prepubertal patients before and after surgery.
  
  
  METHODS
  We examined 82 prepubertal boys 0.6 to 13.2 years old, including 33 with unilateral cryptorchidism, 21 with inguinoscrotal anomalies and 28 who were normal. IgG, IgM and IgA antisperm antibodies were determined by the indirect Immunobead test. Serum testing was repeated 1 and 2 years postoperatively and annually for 2 more years in the normal children. Also sera from 183 infertile men 21 to 47 years old with a history of cryptorchidism and/or inguinal hernia operated on in childhood were similarly studied.
  
  
  RESULTS
  Of the adults 70 (39%) tested IgG positive, including 12 (7%) who were also IgA positive, and all tested IgM negative. Repeat measurements were negative for all IgG, IgA and IgM isotypes in all children, patients and controls.
  
  
  CONCLUSIONS
  We conclude that there are no antibodies to sperm surface antigens in prepubertal children with cryptorchidism and inguinoscrotal anomalies before and within 2 years after surgery. Autoimmunity against postmeiotic sperm membrane antigens is apparent in adults only.
authors.affiliation: Department of Pediatric Surgery, Karamandanion Children's Hospital, Patras, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Mirilas
authors.forename: M
authors.initials: M
authors.lastname: De Almeida
mesh_terms: D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003456:Cryptorchidism / Q000097:blood* / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D011184:Postoperative Period; D011300:Preoperative Care; D011627:Puberty; D013094:Spermatozoa / Q000276:immunology*; D014564:Urogenital Abnormalities / Q000097:blood / Q000276:immunology
chemical_list: D001323:Autoantibodies