title: Molecular cloning and sequence analysis of the cDNA encoding beta-lactoglobulin in Bubalus bubalis.
abstract: The cDNA for bubaline beta-lactoglobulin (beta lg) has been cloned through RT-PCR approach and sequenced. Sequence data showed a single open reading frame coding for a protein of 180 amino acids with a signal sequence of 18 amino acid residues. Comparison with other ruminant beta lg sequences revealed a high homology indicating the protein to be conserved through evolution. The degree of homology, at amino acid level, is 96.1%, 95.6%, 93.9% and 63.7% with goat, sheep, cow and pig, respectively.
authors.affiliation: Gene Regulation Laboratory, National Institute of Immunology, New Delhi, India.
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.forename: S
authors.initials: S
authors.lastname: Jain
authors.forename: S
authors.initials: S
authors.lastname: Nayak
authors.forename: K B
authors.initials: KB
authors.lastname: Apparao
authors.forename: S M
authors.initials: SM
authors.lastname: Totey
authors.forename: L C
authors.initials: LC
authors.lastname: Garg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007782:Lactoglobulins / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D007782:Lactoglobulins