Unique features of dendritic cells in IFN-gamma transgenic mice: relevance to cancer development and therapeutic implications.
Although induction of interferon gamma (IFN-gamma) and activation of antigen presenting dendritic cells (DCs) are two vital events during an immune response, the impact of endogenous IFN-gamma on DC function has yet to be clarified. The phenotype and function of DCs isolated from mice with high (IFN-gamma-transgenic mouse [Tg]) and undetectable levels of circulating IFN-gamma (normal mice [NM]) were therefore compared. The capacity to stimulate allogenic (p < 0.05) and antigen-specific T lymphocytes (p < 0.05), as well as the ability to produce IL-12 (p < 0.05) and to process soluble protein antigens (p < 0.05) was found to be significantly higher in DCs from the Tg mice compared to the NM case. The presence of activated DCs in a microenvironment of endogenous IFN-gamma suggests that the IFN-gamma-Tg mouse is a suitable animal model to study cancer immunotherapy in vivo.
Akbar
S M
SM
Third Department of Internal Medicine, Ehime University School of Medicine, Ehime, 791-0295, Japan.
Kajino
K
K
Tanimoto
K
K
Yamamura
K i
Ki
Onji
M
M
Hino
O
O
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D051940:B7-2 Antigen; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006433:Hemocyanins / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007167:Immunotherapy; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics*; D018664:Interleukin-12 / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D016207:Cytokines; D006514:Hepatitis B Surface Antigens; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D018664:Interleukin-12; D007371:Interferon-gamma; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin