[Detection of mRNA expression of endothelin-1 in organs on severe burn rat model with in situ hybridization technique].
After the dynamic observation of postburn endothelin changes in plasma on severe burn rat model, we detected ET-1 mRNA expression and cell localization in some organs such as heart, lung, liver and kidney with in situ hybridization technique. We that endothelin concentration in plasma rapidly increased postburn (it reached its climax at 6th hour and maintained fairly high level even at 24th hour postburn). Meanwhile in situ hybridization indicated that ET-1 mRNA expression intensity and number of positive cells rapidly increased postburn. The expression climaxes of which appeared decreasing in turn from kidney, liver, lung to heart and the types of positive cells increased. This may due to the endothelin's functions of contracting blood vessels and regulating the redistribution of blood flow and be helpful for guaranteeing blood perfusion of important organs under shock state, but the overexpression of ET-1 mRNA may make the ischemic and anoxic damages to organs more severe.
Wang
X
X
Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing.
Gao
J
J
Luo
Q
Q
D000818:Animals; D002056:Burns / Q000378:metabolism*; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014781:Viscera / Q000378:metabolism*
D019332:Endothelin-1; D012333:RNA, Messenger