title: Nonspecific microvascular vasodilation during iontophoresis is attenuated by application of hyperosmolar saline.
abstract: Iontophoretic administration of acetylcholine chloride (ACh) and sodium nitroprusside (SNP) combined with laser Doppler skin blood perfusion measurements are used for determination of endothelial-dependent and -independent vasodilation. However, the method is biased by nonspecific vasodilation. The primary aim of this study was to investigate if iontophoresis-induced nonspecific vasodilation may be attenuated by addition of high molar concentrations of NaCl to the iontophoresis solutions. Secondary we investigated the applicability of 5 mol/liter NaCl solution as vehicle for ACh and SNP in this method. Skin perfusion changes were determined for iontophoresis of pure vehicles, deionized water and 5 mol/liter NaCl solution, in 12 healthy volunteers. Responses in skin perfusion to iontophoresis of ACh and SNP dissolved in both vehicles were also investigated. Addition of 5 mol/liter NaCl to deionized water significantly attenuated the nonspecific vasodilation and lowered the potential applied over the skin. The inter- and intraindividual coefficients of variation to ACh and SNP responses became, however, higher using hyperosmolar vehicle. During iontophoresis of SNP (in deionized water) we were unable to distinguish between SNP and vehicle effects. This study shows that the nonspecific vasodilation induced by iontophoresis can be attenuated by addition of 5 mol/liter NaCl, possibly due to lower electrical potential over the skin. However, the variability of the method was not improved. When deionized water was used as vehicle the effect of SNP could not be differentiated from that of the vehicle. This was not the case for ACh.
authors.affiliation: Department of Pharmacology, School of Pharmacy, University of Oslo, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Asberg
authors.forename: T
authors.initials: T
authors.lastname: Holm
authors.forename: T
authors.initials: T
authors.lastname: Vassbotn
authors.forename: A K
authors.initials: AK
authors.lastname: Andreassen
authors.forename: A
authors.initials: A
authors.lastname: Hartmann
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000328:Adult; D001783:Blood Flow Velocity / Q000187:drug effects; D003864:Depression, Chemical; D005260:Female; D005542:Forearm / Q000098:blood supply; D006801:Humans; D007478:Iontophoresis; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D009599:Nitroprusside / Q000494:pharmacology; D010477:Perfusion; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012867:Skin / Q000098:blood supply / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*; D014867:Water / Q000494:pharmacology
chemical_list: D012462:Saline Solution, Hypertonic; D014867:Water; D009599:Nitroprusside; D000109:Acetylcholine