The effect of various doses of lazaroid U74389G on lung ischemia reperfusion injury.
BACKGROUND
We investigated the effect of lazaroid U74389G, a potent inhibitor of lipid peroxidation, on ischemia-reperfusion injury at three different doses in the rat orthotopic left lung transplantation model.


METHODS
Four groups of reperfused lungs were studied. In group I (control) donor lungs were transplanted after 12 hours of preservation in University of Wisconsin (UW) solution at 4 degrees C. In groups II, III, and IV, lazaroid was used as an additive to UW solution at concentrations of 30 micromol/L, 50 micromol/L, and 100 micromol/L, respectively, and was also administered intravenously to the recipients 30 minutes before reperfusion after 12 hours of storage in the UW solution at 4 degrees C.


RESULTS
After 1 hour of reperfusion, gas exchange function (p < 0.01), tissue lipid peroxide levels (p < 0.05) and histologic damage in reperfused lung allografts (p< 0.05) were significantly improved in lazaroid-treated group IV compared with control group I.


CONCLUSIONS
These findings suggest that lazaroid U74389G ameliorates ischemia-reperfusion injury in rat lung transplants by inhibiting lipid peroxidation.
Kuwaki
K
K
Department of Thoracic and Cardiovascular Surgery, Sapporo Medical University School of Medicine, Hokkaido, Japan.
Komatsu
K
K
Sohma
H
H
Abe
T
T
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001834:Body Water / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D008168:Lung / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D016040:Lung Transplantation / Q000502:physiology; D008297:Male; D009929:Organ Size; D011281:Pregnatrienes / Q000008:administration & dosage*; D011659:Pulmonary Gas Exchange; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013050:Spectrometry, Fluorescence
D000975:Antioxidants; D008054:Lipid Peroxides; D011281:Pregnatrienes; C074965:U 74389F