title: Isolation and characterization of a sigB deletion mutant of Staphylococcus aureus.
abstract: The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mutant showed no reduction in virulence.
authors.affiliation: SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania, USA.
authors.forename: R O
authors.initials: RO
authors.lastname: Nicholas
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: McDevitt
authors.forename: A
authors.initials: A
authors.lastname: Marra
authors.forename: S
authors.initials: S
authors.lastname: Sucoloski
authors.forename: P L
authors.initials: PL
authors.lastname: Demarsh
authors.forename: D R
authors.initials: DR
authors.lastname: Gentry
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D017353:Gene Deletion; D051379:Mice; D051381:Rats; D012808:Sigma Factor / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C079011:SigB protein, Bacteria; D012808:Sigma Factor