[A mutant allele gam18, participating in the RecF repair path in Escherichia coli K-12].
Plasmid pGam18 carrying one of the cloned mutant loci, responsible for enhanced radiation resistance in the strain Escherichia coli Gamr444, was shown to increase resistance to the lethal effect of gamma-rays with a dose modification factor DMF = 2. Enhanced resistance was observed in wild-type cells and in the mutant recBC sbcB, but not recFBC sbcA. This indicates the involvement of a product of the gam18 locus in the RecF pathway of recombinational repair. The protective effect of plasmid pGam18 against radiation was completely abolished by mutations in the most RecF pathway genes (recF, recJ, recR, recO, recQ, recN, and ruvB). However, three mutations in the uvrD gene, which encodes DNA helicase II and belongs to the RecF pathway, can be partially complemented by plasmid pGam18. These data suggest that the mutant allele gam18 affects the DNA helicase II activity at the presynaptic stage of the RecF pathway-mediated repair of DNA double-stranded breaks induced by gamma-irradiation.
Verbenko
V N
VN
St. Petersburg Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Leningrad Oblast, Russia.
Krup'ian
E P
EP
Kuznetsova
L V
LV
Kalinin
V L
VL
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005720:Gamma Rays; D005798:Genes, Bacterial; D009154:Mutation; D011836:Radiation Tolerance / Q000235:genetics; D011995:Recombination, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C489340:recF protein, E coli; C066218:recF protein, Bacteria
Mutantnyĭ allel' gam18, uchastvuiushchiĭ v RecF-puti reparatsii u Escherichia coli K-12.