5'-Nucleotidase as a marker of both general and local inflammation in rheumatoid arthritis patients.
OBJECTIVE
To evaluate measurements of serum and synovial fluid 5'-nucleotidase (5'N) activity as a marker of general and local inflammation in arthritis, and to resolve a contradiction in the literature as to whether or not the activity of 5'N in the synovial fluids of rheumatoid arthritis (RA) patients is raised in comparison with that in the synovial fluids of other arthritis patients.


METHODS
Assays for 5'N were carried out in the presence of inhibitors of other phosphatases, AMP deaminase and of 5'N itself.


RESULTS
The 5'N activity in the synovial fluid of RA patients was both significantly higher (mean 1.7-fold) and had a greater variance than that in the synovial fluids of other arthritis patients, and the contradiction in the literature was resolved. There was a strong correlation between the 5'N activity in the sera of RA patients and their erythrocyte sedimentation rate. There was no significant correlation between the 5'N in the serum and synovial fluid for the RA patients, in marked contrast to the strong correlation between the two 5'N activities shown by the osteoarthritis patients. The 5'N activity was greater in the synovial fluid than in the serum for virtually all the patients, showing that it was being made locally.


CONCLUSIONS
The 5'N activity in the serum (which came mostly from the liver) could be used as a marker of general inflammation, whereas the 5'N in the synovial fluid was mostly produced locally, and could be used as a marker of joint inflammation, particularly for the RA patients.
Johnson
S M
SM
Division of Biochemistry, St George's Hospital and Medical School, London, UK.
Patel
S
S
Bruckner
F E
FE
Collins
D A
DA
D015720:5'-Nucleotidase / Q000032:analysis* / Q000097:blood; D000659:AMP Deaminase / Q000494:pharmacology; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000175:diagnosis* / Q000201:enzymology* / Q000276:immunology; D015415:Biomarkers; D001799:Blood Sedimentation; D003070:Coformycin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000175:diagnosis / Q000201:enzymology / Q000276:immunology; D013582:Synovial Fluid / Q000201:enzymology* / Q000276:immunology
D015415:Biomarkers; D004791:Enzyme Inhibitors; D003070:Coformycin; D015720:5'-Nucleotidase; D000659:AMP Deaminase