title: A functional bone morphogenetic protein system in the ovary.
abstract: Bone morphogenetic proteins (BMPs) comprise a large group of polypeptides in the transforming growth factor beta superfamily with essential physiological functions in morphogenesis and organogenesis in both vertebrates and invertebrates. At present, the role of BMPs in the reproductive system of any species is poorly understood. Here, we have established the existence of a functional BMP system in the ovary, replete with ligand, receptor, and novel cellular functions. In situ hybridization histochemistry identified strong mRNA labeling for BMP-4 and -7 in the theca cells and BMP receptor types IA, IB, and II in the granulosa cells and oocytes of most follicles in ovaries of normal cycling rats. To explore the paracrine function of this BMP system, we examined the effects of recombinant BMP-4 and -7 on FSH (follicle-stimulating hormone)-induced rat granulosa cytodifferentiation in serum-free medium. Both BMP-4 and -7 regulated FSH action in positive and negative ways. Specifically, physiological concentrations of the BMPs enhanced and attenuated the stimulatory action of FSH on estradiol and progesterone production, respectively. These effects were dose- and time-dependent. Furthermore, the BMPs increased granulosa cell sensitivity to FSH. Thus, BMPs have now been identified as molecules that differentially regulate FSH-dependent estradiol and progesterone production in a way that reflects steroidogenesis during the normal estrous cycle. As such, it can be hypothesized that BMPs might be the long-sought "luteinization inhibitor" in Graafian follicles during their growth and development.
authors.affiliation: Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093-0633, USA. sshimasaki@ucsd.edu
authors.forename: S
authors.initials: S
authors.lastname: Shimasaki
authors.forename: R J
authors.initials: RJ
authors.lastname: Zachow
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Iemura
authors.forename: N
authors.initials: N
authors.lastname: Ueno
authors.forename: K
authors.initials: K
authors.lastname: Sampath
authors.forename: R J
authors.initials: RJ
authors.lastname: Chang
authors.forename: G F
authors.initials: GF
authors.lastname: Erickson
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology*; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology; D010053:Ovary / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000502:physiology*; D017978:Receptors, Growth Factor; D016212:Transforming Growth Factor beta
chemical_list: C525811:Bmp4 protein, rat; C525821:Bmp7 protein, rat; D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D016212:Transforming Growth Factor beta; D005640:Follicle Stimulating Hormone; D052004:Bone Morphogenetic Protein Receptors