title: Ultrastructural changes of the tooth root surface by Nd:YAG laser irradiation followed by citric acid and tetracycline.
abstract: The neodymium:yttrium-aluminum-garnet (Nd:YAG) laser has been used for treatment of dentinal hypersensitivity, eradicating periodontal pathogens, and facilitating calculus removal. However, Nd:YAG laser irradiation exerts potentially harmful effects on the tooth root surface. The purpose of this study was to examine the ultrastructural changes of the tooth root surface caused by Nd:YAG laser irradiation, and to determine whether chemical and mechanical preparations can correct these ultrastructural changes. Eighteen tooth specimens (3 x 3 x 0.5 mm) with healthy root surfaces were prepared and irradiated with an Nd:YAG laser at various power densities. Root surfaces were irradiated at 100 mJ at 20 pulses per second (pps) for 2 seconds followed by the application of citric acid (pH 1.2) or tetracycline solution (100 mg/mL) for 3 and 5 minutes, respectively, or ultrasonic scaling for 5 strokes of 3 seconds per stroke. As observed with low-vacuum scanning electron microscopy, (Wet-SEM), Nd:YAG laser irradiation at 70 to 100 mJ, 20 pps for 2 seconds caused surface cratering, areas of porosity, pitting, fissures, and lava-like structures in an area 140 to 280 microns in diameter. Irradiation of 50 mJ, 20 pps for 2 seconds, led to only mild surface charring. No evidence of morphologic changes was found when root surfaces were irradiated with the Nd:YAG laser at 20 mJ, 20 pps for 2 seconds or at 50 mJ, 10 pps for 8 seconds. The laser-induced lava-like structures were partially detached by citric acid (pH 1.2) etching and ultrasonic scaling, but not by tetracycline (100 mg/mL). These results indicate that chemical and mechanical preparations can be used effectively in conjunction with Nd:YAG laser irradiation for root surface preparation during both nonsurgical and surgical periodontal treatments.
authors.affiliation: Graduate Institute of Clinical Dental Science, College of Medicine, National Taiwan University, Taipei.
authors.forename: J H
authors.initials: JH
authors.lastname: Jeng
authors.forename: K W
authors.initials: KW
authors.lastname: Chen
authors.forename: C P
authors.initials: CP
authors.lastname: Lin
authors.forename: H G
authors.initials: HG
authors.lastname: Chou
authors.forename: W H
authors.initials: WH
authors.lastname: Lan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D013752:Tetracycline / Q000494:pharmacology*; D014092:Tooth Root / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure*
chemical_list: D000900:Anti-Bacterial Agents; D019343:Citric Acid; D013752:Tetracycline