A new enzyme immunoassay for PCDD/F TEQ screening in environmental samples: comparison to micro-EROD assay and to chemical analysis.
A new Enzyme ImmunoAssay (EIA) for PCDD/F TEQ measurement in extracts of environmental samples was described. The bioassay TEQ which derived from EIA and EROD were compared with each other and with results from chemical analysis. For all environmental samples, the EROD-TEQ is higher than the value from chemical analysis. However, the EIA-TEQ is much more identical with the value from chemical analysis. Our results indicate that the EIA assay is a complementary method to the EROD assay and should be useful as a rapid and sensitive screening tool for environmental samples in many situations.
Li
W
W
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P.R. China.
Wu
W Z
WZ
Barbara
R B
RB
Schramm
K W
KW
Kettrup
A
A
D001572:Benzofurans / Q000032:analysis*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000592:standards; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*
D001572:Benzofurans; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants; D019363:Cytochrome P-450 CYP1A1