Expression of a Cx43 deletion mutant in 3T3 A31 fibroblasts prevents PDGF-induced inhibition of cell communication and suppresses cell growth.
Communication through gap junctions was first suggested to have a role in the social control of cell growth over 30 years ago. However, despite extensive experimentation, the importance of gap junctions as a general mechanism of growth control remains to be established. A number of different studies have shown that a common early response of cells in culture to polypeptide growth factors such as PDGF is a rapid and transient inhibition of cell communication suggesting that a cell may have to lose communication with its neighbors before it can undergo cell division. Here we show that 3T3 A31 fibroblasts exposed to PDGF exhibit a 50% decrease in cell communication as measured by dye transfer in the absence of significant changes in the cellular content and distribution of Cx43. Likewise, PDGF inhibited cell communication in cells transfected either with a vector which did not contain a cDNA or with an expression vector encoding full-length Cx43 fused to a c-myc tag (Cx43-M). In contrast, 3T3 A31 fibroblasts transfected with an expression construct encoding a deletion mutant of Cx43 (Cx43-256M) consisting of amino acids 1-256 of Cx43 fused to a c-myc tag maintain high levels of gap junction activity following exposure to PDGF. These results suggest that sites which trigger loss of cell communication in response to PDGF are located within amino acids 257 to 382 of the Cx43 molecule. Cells transfected with an expression vector encoding full-length Cx43 fused to a c-myc tail exhibited a reduced basal growth rate compared to both parent cells and cells transfected with a control vector but maintained a strong mitogenic response to PDGF. In contrast, both the basal growth rate and the mitogenic response to PDGF was markedly reduced in cells which expressed Cx43-256M consistent with the hypothesis that loss of cell communication is required before a cell can respond to mitogenic stimuli.
Moorby
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Centre for Mechanisms of Human Toxicity, Leicester University, Leicester, LE1 9HN UK. cdm5@le.ac.uk
Gherardi
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D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication / Q000235:genetics*; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D018031:Connexin 43 / Q000096:biosynthesis / Q000235:genetics*; D017629:Gap Junctions / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D009154:Mutation / Q000235:genetics*; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D014162:Transfection
D018031:Connexin 43; D010982:Platelet-Derived Growth Factor; D020033:Protein Isoforms