title: Study to assess the reliability of a disc diffusion method for determining the sensitivity of gram-positive pathogens to dalfopristin/quinupristin.
abstract: A standardized method of disc testing the sensitivity of gram-positive pathogens to dalfopristin/quinupristin was developed, and then 'field tested' in ten centres in the UK. For a 15 microg disc, zone diameter breakpoints of 20 mm and 15 mm are suggested when organisms are tested on Iso-Sensitest agar and Iso-Sensitest agar supplemented with 5% whole horse blood, respectively.
authors.affiliation: Department of Medical Microbiology, City Hospital NHS Trust, Birmingham, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Andrews
authors.forename: J P
authors.initials: JP
authors.lastname: Ashby
authors.forename: R
authors.initials: R
authors.lastname: Wise
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001769:Blood; D003470:Culture Media; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results; D014769:Virginiamycin / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D014769:Virginiamycin; C113825:quinupristin; C113826:dalfopristin