Localization of TFF3 peptide to porcine conjunctival goblet cells.
TFF-peptides (formerly P-domain peptides, trefoil factors) form a new family of mucin-associated peptides mainly in the gastrointestinal tract. TFF3 is a typical secretory product of intestinal goblet cells and occurs also in the respiratory tract. Here, polyclonal antisera specific for TFF3 were used in Western blot analysis and immunofluorescence to determine the presence and distribution of TFF3 in the porcine conjunctiva, which is the primary source for ocular mucins. Significant accumulation of TFF3 was detected in conjunctival goblet cells but not in the lacrimal glands. This peptide, together with ocular mucins, may play a role in the rheological function of the tear film.
Jagla
W
W
Institut für Molekularbiologie und Medizinische Chemie, Universitätsklinikum, Otto-von-Guericke Universität, Leipziger Strasse 44, D-39120 Magdeburg, Germany.
Wiede
A
A
Hoffmann
W
W
D000818:Animals; D015153:Blotting, Western; D003228:Conjunctiva / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D020397:Goblet Cells / Q000378:metabolism*; D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; D011506:Proteins / Q000378:metabolism*; D013552:Swine; D000071165:Trefoil Factor-3
D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; D011506:Proteins; C082495:TFF3 protein, human; D000071165:Trefoil Factor-3