The mRNA-binding ribosomal protein S26 as a molecular marker in plants: molecular cloning, sequencing and differential gene expression during environmental stress.
One condition for using a gene as a transcriptional marker for environmental stress is its specific and differential expression. In order to be used as such a marker, the ribosomal protein S26 cDNA from pea (Pisum sativum L.) was cloned and fully sequenced. The gene (PsRPS26) was shown to be differentially regulated by ozone and UV-B radiation in opposite ways. Ozone gave rise to increased mRNA levels, whereas UV-B led to a decrease in S26 transcript abundance. Thus, the expression of PsRPS26 can be used as a molecular marker to differentiate between these two environmental stresses.
Brosché
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M
Biokemi och Biofysik, Göteborgs Universitet, P.O. Box 462, SE-40530, Göteborg, Sweden.
Strid
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A
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D012269:Ribosomal Proteins