A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye.
To understand molecular mechanisms underlying the response to pressure in human trabecular cells of the eye, genes induced by cyclic mechanical stretching were isolated by subtractive hybridization assisted by polymerase chain reaction. A novel gene containing an Alu repetitive element in the 5' untranslated region was identified, and its expression was confirmed by Northern blot analysis to be stretch-specific in trabecular cells. The gene was also expressed in the retina, but not in the other tissues, including the brain. The gene encoded a putative small protein with 44 amino acids, which showed homology with neuromedin K. The putative novel protein was named as "oculomedin," and would be used as a candidate gene for glaucoma.
Sato
Y
Y
Department of Ophthalmology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama City, 700-8558, Japan.
Matsuo
T
T
Ohtsuki
H
H
D020121:5' Untranslated Regions / Q000235:genetics; D020087:Alu Elements; D000595:Amino Acid Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005136:Eye Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005901:Glaucoma / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D015287:Neurokinin B / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D012160:Retina / Q000378:metabolism; D016415:Sequence Alignment; D013314:Stress, Mechanical; D014129:Trabecular Meshwork / Q000378:metabolism*
D020121:5' Untranslated Regions; D005136:Eye Proteins; C119566:OCLM protein, human; D012333:RNA, Messenger; D015287:Neurokinin B