title: Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664: implication of a novel set of cellulose synthase genes.
abstract: Three sets of cellulose synthase genes were cloned from a cellulose-producing bacterium Acetobacter xylinum JCM 7664. One set of genes (bcsAI/bcsBI/bcsCI/bcsDI) were highly conserved with the well-established type I genes in other strains of A. xylinum, while the other two (bcsABII-A, bcsABII-B) were homologous to the known type II (acsAII). Unexpectedly, they were immediately followed by a gene cluster of bcsX/bcsY/bcsCII/ORF569, likely forming an operon. Western blotting demonstrated that the BcsY protein accumulated in cells. Since BcsY showed striking similarities to a number of membrane-bound transacylases, it was hypothesized that the type II cellulose synthase produces acylated cellulose, which might be anchored on the cytoplasmic membrane. An insertion sequence of IS1380-type was found just upstream of the one type II gene (bcsABII-B), suggestive of nonfunctioning.
authors.affiliation: Energy and Environment R&D Center, Tokyo Electric Power Company, Yokohama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Umeda
authors.forename: A
authors.initials: A
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Ishibashi
authors.forename: H
authors.initials: H
authors.lastname: Akiyama
authors.forename: T
authors.initials: T
authors.lastname: Onizuka
authors.forename: M
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005798:Genes, Bacterial; D041822:Gluconacetobacter xylinus / Q000201:enzymology / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D005964:Glucosyltransferases; C457769:PRC1 protein, Arabidopsis; C028634:cellulose synthase (UDP-forming)