Expression and localization of p80 and p68 interleukin-1 receptor proteins in the brain of adult mice.
The biological effects of interleukin-1 (IL-1) are mediated by two distinct receptors, the p80 type I IL-1 and p68 type II IL-1 receptor proteins (IL-1RI and IL-1RII, respectively), both of which have been recently co-localized to the growth hormone synthesizing cells of the adenohypophysis. Previous studies have shown that IL-1 can bind to specific structures in the central nervous system, but the distribution of IL-1RI and IL-1RII proteins in the adult mouse brain has not been reported. Here we have used immunohistochemistry to study the expression, distribution and cellular localization of both isoforms of the IL-1 receptor proteins in the adult mouse brain. Using a combination of processing techniques (AMeX fixation and cryosectioning), we have immunolabeled brain sections for each isoform of the IL-1R. Both isoforms are expressed in the CNS, particularly in neuronal soma of the granular layer of the dentate gyrus and pyramidal cells of fields CA1-CA4 of Ammon's horn of the hippocampus, in epithelial cells of the choroid plexus and ependymal layer, and in neuronal soma of Purkinje cells of the cerebellum. The IL-1RII isoform, but not IL-1RI, is expressed in specific neuronal soma and proximal cell processes of neurons of the paraventricular gray matter of the hypothalamus. These immunohistochemical data directly demonstrate the neuronal expression of both IL-1R proteins in situ. The distribution and cellular localization of IL-1R proteins in the CNS provide a molecular basis for understanding reciprocal interactions between the immune system and the brain.
French
R A
RA
Department of Pathobiology, College of Agriculture, University of Connecticut, Storrs 06269, USA.
VanHoy
R W
RW
Chizzonite
R
R
Zachary
J F
JF
Dantzer
R
R
Parnet
P
P
Bluthé
R M
RM
Kelley
K W
KW
D000367:Age Factors; D000818:Animals; D001923:Brain Chemistry / Q000276:immunology*; D002831:Choroid Plexus / Q000737:chemistry / Q000276:immunology; D004805:Ependyma / Q000737:chemistry / Q000276:immunology; D005260:Female; D006624:Hippocampus / Q000737:chemistry / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009443:Neuritis / Q000276:immunology / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II
D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II