[Inhibition of lipoxygenase activity reduces radiation-induced DNA fragmentation in lymphocytes].
Lypoxygenase activity of lymphocytes towards exogenous substrate linolic acid and the level of endogenous arachidonate metabolite 15-HETE are increased within 6 h and decreased below the control level at 24 h after 1 Gy X-ray irradiation of Wistar rats. DNA fragmentation is stimulated both in vivo within 12 h and during in vitro incubation of lymphocytes, obtained 3 h following irradiation. DNA analysis by agarose gel shows that DNA fragments are multiples of the 200 base pair unit. When 20 microM nordihydroguayaretic acid, a selective inhibitor of arachidonate lypooxidation, was added to the incubation medium, DNA fragmentation was observed to be significantly less, especially at the early steps of incubation.
Matyshevskaia
O P
OP
Kyiv Taras Shevchenko University, Department of Biochemistry.
Pastukh
V N
VN
Solodushko
V A
VA
D000818:Animals; D053938:DNA Fragmentation / Q000187:drug effects* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D014916:Whole-Body Irradiation
D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D008084:Lipoxygenase
Ingibirovanie lipoksigenaznoĭ aktivnosti snizhaet indutsirovannuiu radiatsieĭ fragmentatsiiu DNK limfotsitov.