Mild vitamin A deficiency delays fetal lung maturation in the rat.
During late pregnancy, the fetal lung stores surfactant in preparation for extrauterine life. Surfactant deficiency, most often due to prematurity, precipitates respiratory distress syndrome (RDS) of the neonate. Although vitamin A (retinol) and retinoic acid have been shown to enhance the synthesis of phospholipid surfactant components, their effect on surfactant-specific proteins is unclear. No attempt has been made to evaluate the consequences of vitamin A restriction on surfactant phospholipid storage or on the expression of the life-essential surfactant protein-B (SP-B). We induced in rats a partial vitamin A deficiency leading to a 30-60% reduction in blood retinol, a status compatible with maintenance of gestation and absence of gross abnormalities in offspring. At term, lung surfactant phospholipids were reduced by 21%, and the major surfactant phospholipid, disaturated phosphatidylcholine (DSPC), was reduced by 27% in vitamin A-deficient (VAD) fetuses. The decrease in surfactant phospholipids and DSPC correlated linearly with plasma retinol, and reached about 50% in fetuses with the lowest retinol concentrations; it was accompanied by reduced expression of the gene for fatty acid synthase, a key enzyme in the synthetic pathway for surfactant-phospholipid lipid precursors. The amounts of SP-A, SP-B, and SP-C messenger RNAs were decreased by 46%, 32%, and 28%, respectively, in VAD fetuses. Consistently, amounts of SP-A and SP-B proteins were diminished as assessed by Western blotting. The proportion of type II cells determined after SP-B labeling was unchanged in VAD as compared with control lungs. Vitamin A deficiency is therefore a cause of lung maturational delay. In view of its rather large incidence in human populations, it may represent an increased risk for RDS and an aggravating factor for prematurity.
Chailley-Heu
B
B
INSERM U319, Développement Normal et Pathologique des Fonctions Epithéliales, Université Paris 7-Denis Diderot, Paris, France.
Chelly
N
N
Lelièvre-Pégorier
M
M
Barlier-Mur
A M
AM
Merlet-Bénichou
C
C
Bourbon
J R
JR
D000818:Animals; D019916:Choline-Phosphate Cytidylyltransferase / Q000032:analysis; D004224:Diterpenes; D005260:Female; D008168:Lung / Q000196:embryology* / Q000201:enzymology; D010743:Phospholipids / Q000032:analysis; D011247:Pregnancy; D011248:Pregnancy Complications; D011270:Pregnancy, Animal; D011663:Pulmonary Surfactants / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D014802:Vitamin A Deficiency / Q000196:embryology*
D004224:Diterpenes; D010743:Phospholipids; D011663:Pulmonary Surfactants; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; D019916:Choline-Phosphate Cytidylyltransferase