title: Quantitative assessment of human leukocyte antigen-G protein in amniotic fluid by a double-determinant enzyme-linked immunosorbent assay using anti-human leukocyte antigen-G-specific antibody '87G'.
abstract: OBJECTIVE
  The objective of this study was to establish an enzyme-linked immunosorbent assay (ELISA) system, in an attempt to quantify the amount of human leukocyte antigen (HLA)-G protein in amniotic fluid.
  
  
  METHODS
  We established a double-determinant ELISA system using the anti-HLA-G specific mouse monoclonal antibody '87G' as a capture antibody and the horseradish-peroxidase labeled rabbit anti-human beta2-microglobulin antibody as a detection antibody. We then measured the concentration of HLA-G protein in amniotic fluid samples from nine normal second-trimester pregnant women and in serum samples from eight normal males.
  
  
  CONCLUSIONS
  HLA-G protein was detected in amniotic fluid at a concentration of 275 ng/ml (197-343 ng/ml) (median value and 95% confident range), whereas the concentration of HLA-G protein in male serum was below the minimum detection level.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hamai
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: A
authors.initials: A
authors.lastname: Miki
authors.forename: D E
authors.initials: DE
authors.lastname: Geraghty
authors.forename: I
authors.initials: I
authors.lastname: Harada
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
authors.forename: S
authors.initials: S
authors.lastname: Kozuma
authors.forename: O
authors.initials: O
authors.lastname: Tsutsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006680:HLA Antigens / Q000032:analysis* / Q000097:blood / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000097:blood / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D014327:Trophoblasts / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I