Chemical stability of silicone oil in the human eye after prolonged clinical use.
OBJECTIVE
To investigate the assumption that silicone oil is chemically stable in the human eye after prolonged clinical use as a vitreous substitute.


METHODS
Experimental study.


METHODS
Samples of silicone oil recovered from the vitreous cavities of 25 consecutive patients up to 26 months (mean, 9.2 months) after implantation and 4 different batches of original highly purified silicone oil with a kinematic viscosity of 5000 mPa.s were analyzed. Visible silicone oil emulsification was present in 18 of the 25 eyes.


METHODS
Gas chromatography/mass spectroscopy (GC/MS) was used to detect and characterize low-molecular weight components (LMWC). Gel permeation chromatography (GPC) was used to determine the molecular weight distribution of the silicone oils. Functional groups of the silicone oils were quantified and characterized by infrared (IR) spectroscopy and 1H nuclear magnetic resonance (1H NMR) spectroscopy.


METHODS
The sample oils explanted from the eyes were compared to the original oils with regard to LMWC, molecular weight distribution, and type and amount of functional groups.


RESULTS
The GC/MS chromatograms showed no peaks indicative of LMWC in any of the 25 sample oils explanted from the eyes or any of the original oils. In the GPC chromatograms, the peak position occurred at the same retention times with identical signal shape in all original and sample oils, indicating that the molecular weight and the molecular weight distribution did not change after prolonged implantation. The IR spectroscopy and 1H NMR spectroscopy showed characteristic absorption bands at 1260 cm(-1) related to symmetric deformation vibration of the Si-CH3 group and at 800 cm(-1) related to the Si-(CH3)2 group. This was identical for all sample and original oils.


CONCLUSIONS
Highly purified 5000-mPa.s silicone oils, used as prolonged retinal tamponades in patients with proliferative vitreoretinopathy, proliferative diabetic retinopathy, and tractional retinal detachment after central retinal vein occlusion, are chemically stable in the human eye and do not undergo chemical modification. The LMWC do not play a substantial role in the variations of oil emulsification.
Lakits
A
A
Department of Ophthalmology, University of Vienna, Austria. adalbert.lakits@akh-wien.ac.at
Nennadal
T
T
Scholda
C
C
Knaus
S
S
Gruber
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002850:Chromatography, Gel; D004322:Drainage; D004355:Drug Stability; D004655:Emulsions; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D012164:Retinal Diseases / Q000601:surgery*; D012827:Silicone Oils / Q000737:chemistry* / Q000627:therapeutic use; D013055:Spectrophotometry, Infrared; D014783:Viscosity
D004655:Emulsions; D012827:Silicone Oils