Deletion of 24 open reading frames from chromosome XI from Saccharomyces cerevisiae and phenotypic analysis of the deletants.
As a part of the EUROFAN program, 24 open reading frames from Saccharomyces cerevisiae (YKR010c to YKR013w, YKR015c to YKR025w, YKR081c to YKR083c, YKR087c to YKR091w and YKR096w) were disrupted in two genetic backgrounds, FY1679 and W303. Systematic deletions and phenotypic analysis were performed following a hierarchical strategy, the so-called 'mass murder'. Of the 24 genes thus deleted, four are essential, whereas the deletion of 17 did not reveal any significant difference between the parental and mutant strains. Deletions of the remaining three show some growth phenotype; ykr024c mutants grow slowly under any conditions, ykr019c mutants grow slower in a rich medium and ykr082w mutants are temperature sensitive, being unable to germinate at 30 degrees C and above.
Zúñiga
S
S
Centro de Biología Molecular 'Severo Ochoa', CSIC and UAM, 28049-Cantoblanco, Madrid, Spain.
Boskovic
J
J
García-Cantalejo
J M
JM
Jim nez
A
A
Ballesta
J P
JP
Remacha
M
M
D001483:Base Sequence; D015825:Chromosomes, Fungal; D017931:DNA Primers; D006238:Haploidy; D006358:Hot Temperature; D009154:Mutation; D016366:Open Reading Frames; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D017384:Sequence Deletion
D017931:DNA Primers