Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium.
An acid-stable soybean-peroxidase biosensor was developed by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 microA/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA).
Wang
B
B
Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, PRC.
Li
B
B
Wang
Z
Z
Xu
G
G
Wang
Q
Q
Dong
S
S
D004800:Enzymes, Immobilized / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016877:Oxidants / Q000032:analysis*; D010544:Peroxidases / Q000737:chemistry*; D013025:Glycine max / Q000201:enzymology*
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D016877:Oxidants; D006861:Hydrogen Peroxide; D010544:Peroxidases