A protective role for common p21WAF1/Cip1 polymorphisms in human ovarian cancer.
The objective of this study was to compare the frequency of two common p21WAF1/Cip1 gene polymorphisms in ovarian cancer patients with that in age-matched controls, from a population originating from Eastern Scotland. Both polymorphisms were found significantly less frequently in both the constitutive and tumour tissue DNA of ovarian cancer patients (3/65; 4.6%), than in that from geographically and age-matched controls (25/186; 13.4%) (p=0.0495, chi2). Furthermore, we found no p21WAF1/Cip1 gene mutations in any of the tumours, reflected by a relatively low degree of loss of heterozygosity (LOH) at the chromosomal region where the gene maps, providing further evidence that the p21WAF1/Cip1 gene is not mutated in ovarian cancer. The data suggest however, that there may potentially be a protective function for the two p21WAF1/Cip1 gene polymorphisms in the population under study.
Milner
B J
BJ
Department of Medicine and Therapeutics (Medical Genetics), Aberdeen University Medical School, Foresterhill, Aberdeen AB25 2ZD, UK.
Brown
I
I
Gabra
H
H
Kitchener
H C
HC
Parkin
D E
DE
Haites
N E
NE
D000483:Alleles; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics* / Q000502:physiology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D018450:Disease Progression; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D009363:Neoplasm Proteins / Q000235:genetics* / Q000502:physiology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012606:Scotland / Q000453:epidemiology
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins