title: [A study of a derivative of Taq DNA polymerase].
abstract: Using the method of double primer oligonucleotide-mediated mutagenesis, the high expression plasmid of TaqND236, a derivative of Taq DNA polymerase, was constructed. To determine the frameshift mutation frequency of the in vitro DNA synthesis, we constructed a Gapped-DNA system using the pFDPM118 (a mutant of pUC118 with a -1 frameshift mutation on the lacZ gene) as template. By calculating the ratio of blue and white colonies on the X-gal plate after transforming E. coli TG1, the frameshift mutation frequency of Taq and TaqND236 was measured. It was found that the replication fidelity of the deleted Taq-TaqND236 increased more than 10 folds.
authors.affiliation: Institute of Genetics, Fudan University, Shanghai.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: C N
authors.initials: CN
authors.lastname: Ji
authors.forename: H S
authors.initials: HS
authors.lastname: Du
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zheng
authors.forename: Y M
authors.initials: YM
authors.lastname: Mao
mesh_terms: D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D016368:Frameshift Mutation; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism*
chemical_list: D019914:Taq Polymerase