Protective capacity of a IgM/IgA-enriched polyclonal immunoglobulin-G preparation in endotoxemia.
Animal experiments were carried out to investigate whether a protective effect can be achieved in endotoxemia by intravenous (i.v.) application of a polyclonal immunoglobulin preparation (IVIG-IgG/A/M) enriched with 12% IgM and 12% IgA. Following administration of IVIG-IgG/A/M (500 mg/kg), endotoxemia was induced by intraperitoneal inoculation of a sublethal dose (5x10(8) CFU/kg) of Escherichia coli (E. coli) and subsequent i.v. administration of an antimicrobial agent (Imipenem). Plasma endotoxin activity, IL-6 activity, mean arterial pressure, and skeletal muscle oxygen pressure (tpO2) were measured at regular intervals over a total observation period of 7 h. Prophylactic administration of IVIG-IgG/A/M was found to significantly attenuate (P<0.01) the antibiotic-induced increase in endotoxin activity as compared to the albumin control group. Limited endotoxemia in the IgG/A/M group was associated with reduced levels of circulating IL-6 (P<0.01). Both lipopolysaccharide-induced hypotension and depression of tissue oxygenation were attenuated (P<0.01) by pre-treatment with IVIG-IgG/A/M. The experimental results suggest that in endotoxemia the polyclonal immunoglobulin preparation has a prophylactic protective effect on the acute phase responses and reduces the cardiodepressant effects of E. coli septicaemia.
Oesser
S
S
Department of General Surgery and Thoracic Surgery, University of Kiel, Germany.
Schulze
C
C
Seifert
J
J
D000818:Animals; D001431:Bacteriological Techniques; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D019446:Endotoxemia / Q000097:blood / Q000628:therapy*; D004926:Escherichia coli; D007022:Hypotension / Q000097:blood / Q000517:prevention & control / Q000628:therapy; D007070:Immunoglobulin A / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000494:pharmacology; D007075:Immunoglobulin M / Q000494:pharmacology*; D016756:Immunoglobulins, Intravenous; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000097:blood / Q000494:pharmacology; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology; D010100:Oxygen / Q000032:analysis; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016756:Immunoglobulins, Intravenous; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010100:Oxygen