Inhibitory effects of Sasa senanensis Rehder extract (SE) on calcium-ionophore A23187-induced histamine release from rat peritoneal exudate cells.
The effects of Sasa senanensis Rehder extract (SE) by alkaline hydrolysis on histamine release from rat peritoneal exudate cells (PECs) were examined. Preincubation with SE for 5 min suppressed calcium-ionophore A23187-induced histamine release in a concentration-dependent manner. A23187 evoked a quick increase in cytoplasmic free calcium ([Ca2+]i) levels in the presence of extracellular calcium. Preincubation with SE also had an inhibitory effect on calcium influx increases induced by A23187. These results indicate that SE prevents degranulation from rat PECs by inhibiting [Ca2+]i level elevation.
Okazaki
M
M
Department of Pharmacology, School of Medicine, Showa University, Hatanodai, Tokyo, Japan.
Tsuji
M
M
Yamazaki
Y
Y
Kanda
Y
Y
Iwai
S
S
Oguchi
K
K
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005122:Exudates and Transudates / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D007476:Ionophores / Q000494:pharmacology*; D007501:Iron / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D018613:Microscopy, Confocal; D010537:Peritoneum; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018927:Anti-Asthmatic Agents; D007476:Ionophores; D010936:Plant Extracts; D000001:Calcimycin; D003300:Copper; D007501:Iron; D004205:Cromolyn Sodium; D002118:Calcium