Induction of human leukocyte antigen (HLA)-A2-restricted and MAGE-3-gene-derived peptide-specific cytolytic T lymphocytes using cultured dendritic cells from an HLA-A2 esophageal cancer patient.
OBJECTIVE
Using peripheral blood mononuclear cells (PBMCs) from a 10-year survivor with established human leukocyte antigen (HLA)-A2(+) and MAGE-3(+) esophageal cancer cell line (KYSE-170), we examined the induction of HLA-A2-restricted and MAGE-3-gene-derived peptide (FLWGPRALV, amino acids 271-279)-specific cytolytic T lymphocytes (CTLs).


METHODS
Autologous dendritic cells (DCs) cultured with granulocyte-macrophage colony stimulating factor and interleukin-4 were used as antigen presenting cells. PBMCs were stimulated by peptide-pulsed DCs in vitro.


RESULTS
PBMC cocultured with FLWGPRALV-pulsed DCs could induce the relevant peptide-specific CTLs, which had tumor necrosis factor production and specific cytotoxicity against relevant peptide-pulsed autologous DCs (34%, effector:target ratio = 40:1). Moreover, they showed specific cytotoxicity against the autologous esophageal cancer cell line KYSE-170 (17%, effector:target ratio = 40:1).


CONCLUSIONS
These results suggest that FLWGPRALV-pulsed cultured DCs would be a potent candidate for peptide vaccine against HLA-A2(+) and MAGE-3(+) esophageal cancer.
Kanaoka
S
S
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan. kanaoka@kuhp.kyoto-u.ac.jp
Yamasaki
S
S
Okino
T
T
Inoue
N
N
Shimada
Y
Y
Kaneko
M
M
Otaka
A
A
Fujii
N
N
Imamura
M
M
D000938:Antigen-Presenting Cells / Q000276:immunology; D000951:Antigens, Neoplasm; D003713:Dendritic Cells / Q000276:immunology; D004938:Esophageal Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D015789:HLA-A2 Antigen; D006801:Humans; D016233:Immunotherapy, Active; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D010455:Peptides; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; D036341:Intercellular Signaling Peptides and Proteins; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins; D010455:Peptides; C075525:A2-binding peptide