cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase and cis-1, 2-dihydro-1,2-dihydroxynaphathalene dehydrogenase catalyze dehydrogenation of the same range of substrates.
Pseudomonas putida strain G7 cis-1,2-dihydro-1, 2-dihydroxynaphthalene dehydrogenase (NahB) and Comamonas testosteroni strain B-356 cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase (BphB) were found to be catalytically active towards cis-2,3-dihydro-2,3-dihydroxybiphenyl (specificity factors of 501 and 5850 s-1 mM-1 respectively), cis-1,2-dihydro-1, 2-dihydroxynaphthalene (specificity factors of 204 and 193 s-1 mM-1 respectively) and 3,4-dihydro-3,4-dihydroxy-2,2',5, 5'-tetrachlorobiphenyl (specificity factors of 1.6 and 4.9 s-1 mM-1 respectively). A key finding in this work is the capacity of strain B-356 BphB as well as Burkholderia cepacia strain LB400 BphB to catalyze dehydrogenation of 3,4-dihydro-3,4-dihydroxy-2,2',5, 5'-tetrachlorobiphenyl which is the metabolite resulting from the catalytic meta-para hydroxylation of 2,2',5,5'-tetrachlorobiphenyl by LB400 biphenyl dioxygenase.
Barriault
D
D
INRS-Santé, Institut national de la recherche scientifique, 245 Hymus Boulevard, Pointe-Claire, Québec, H9R 1G6, Canada.
Vedadi
M
M
Powlowski
J
J
Sylvestre
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D016956:Burkholderia cepacia / Q000201:enzymology; D007700:Kinetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010802:Phylogeny; D016958:Pseudomonas putida / Q000201:enzymology; D013997:Time Factors
D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase