Human immune response to a peptide mimic of Neisseria meningitidis serogroup C in hu-PBMC-SCID mice.
An anti-idiotype-based peptide mimic vaccine for Neisseria meningitidis serogroup C polysaccharide (MCPS) has been developed and shown to induce a response in mice that is specific, functional, and T-dependent. In this study, the immunogenicity of the MCPS peptide mimic vaccine preparation, as a potential vaccine for use in humans, is shown using the hu-PBMC-SCID mouse model. The human antibody response to the MCPS peptide mimic vaccine is specific and functional as shown by inhibition enzyme-linked immunoadsorbent assay (ELISA) and bactericidal assay. These data support the usefulness of the peptide mimic vaccine strategy for humans.
Hutchins
W A
WA
Department of Medicine, Medical College of Ohio, Toledo 43699, USA.
Kieber-Emmons
T
T
Carlone
G M
GM
Westerink
M A
MA
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002678:Chimera / Q000276:immunology; D000939:Epitopes; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D022401:Meningococcal Vaccines; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D020449:Repetitive Sequences, Amino Acid; D014611:Vaccination
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D022401:Meningococcal Vaccines; D010446:Peptide Fragments; D011135:Polysaccharides, Bacterial; C055966:immunoglobulin Fv; C037676:meningococcal group C polysaccharide