title: Sodium-bicarbonate cotransport in retinal astrocytes and Müller cells of the rat.
abstract: Sodium-bicarbonate cotransport in retinal glial cells was studied in the everted eyecup preparation of the rat. Intracellular pH was monitored with the indicator dye BCPCF and fluorescence confocal microscopy. Raising the K+ concentration from 3 to 12 mM in HCO3- -buffered perfusate evoked an intracellular alkalinization in both astrocytes and Müller cells. The alkalinization developed more rapidly and was larger in astrocytes. The K+ -induced alkalinization was HCO3- -dependent; it was reduced by 33% in astrocytes and 71% in Müller cells when HCO3- was removed from the perfusate. The alkalinization was effectively blocked by addition of 0.5 mM 4,4"-diisothiocyanato-stilbene-2,2'-disulfonic acid (DIDS). Removal of Na+ from the perfusate evoked a rapid acidification in both types of glial cells. The results indicate that astrocytes and Müller cells in situ in the rat retina possess an electrogenic Na+/HCO3- cotransporter.
authors.affiliation: Department of Physiology, University of Minnesota, Minneapolis 55455, USA. ean@tc.umn.edu
authors.forename: E A
authors.initials: EA
authors.lastname: Newman
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid; D008297:Male; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D017693:Sodium Bicarbonate / Q000378:metabolism*; D027982:Sodium-Bicarbonate Symporters
chemical_list: D001639:Bicarbonates; D002352:Carrier Proteins; D027982:Sodium-Bicarbonate Symporters; D017693:Sodium Bicarbonate; D012964:Sodium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium