[Molecular mechanism of diapause in Bombyx mori. 2. Diapause determination and expression of diapause hormone gene in pupal stage].
Embryonic diapause in Bombyx is induced by diapause hormone (DH), which is produced in the suboesophageal ganglion from a precursor protein. We have cloned the DH cDNA into pBluescript KS vector that containing T7 RNA promoter can be used to synthesize nonspecific RNA. DH cRNA was synthesized and used as internal control. We quantitatively measured DH mRNA at SG of day 3 pupae by Northern hybridization analysis and suggested that the embryonic diapause of Bombyx in next generation was determined by DH mRNA content in SG.
Xu
W H
WH
Department of Biology, University of Science and Technology of China, Hefei.
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D007301:Insect Hormones / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D018075:RNA, Complementary / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*
D007301:Insect Hormones; D009479:Neuropeptides; D018075:RNA, Complementary; D012333:RNA, Messenger; C075027:diapause hormone