Molecular analysis of a new splice variant of the human melanocortin-1 receptor.
The primary hormonal regulator of pigmentation is melanocyte stimulating hormone derived from proopiomelanocortin by proteolytic processing. The melanocortin-1 receptor serves a key role in the regulation of pigmentation. We describe the identification of the first intron within a melanocortin receptor. A new melanocortin-1 receptor isoform, generated by alternative mRNA splicing, encodes an additional 65 amino acids at the predicted intracellular, C-terminal tail of the melanocortin-1 receptor. When expressed in heterologous cells, the new spliced form of the melanocortin-1 receptor (melanocortin-1 receptor B) appears pharmacologically similar to the non-spliced melanocortin-1 receptor. Melanocortin-1 receptor B is expressed in testis, fetal heart and melanomas.
Tan
C P
CP
Department of Metabolic Disorders, Merck Research Laboratories, Rahway, NJ 07065, USA.
McKee
K K
KK
Weinberg
D H
DH
MacNeil
T
T
Palyha
O C
OC
Feighner
S D
SD
Hreniuk
D L
DL
Van Der Ploeg
L H
LH
MacNeil
D J
DJ
Howard
A D
AD
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D020224:Expressed Sequence Tags; D006801:Humans; D020128:Inhibitory Concentration 50; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011485:Protein Binding; D018043:Receptors, Corticotropin / Q000235:genetics* / Q000378:metabolism; D044101:Receptors, Melanocortin
D018043:Receptors, Corticotropin; D044101:Receptors, Melanocortin