Protective effect of swerchirin on hematopoiesis in 60Co-irradiated mice.
The protective effect of swerchirin, a purified xanthone isolated from whole herb of Swertia calycina Franch. on hematopoiesis was investigated. A significant increase of colony formation in the spleen (colony forming unit in spleen = CFU-S) of mice irradiated with 550 rad 60Co gamma-rays and an enhancement of proliferative response of BMC to rmGM-CSF treated with swerchirin [10 mg/kg, 3 time/wk, i.p.] was observed. After introduction of swerchirin [10 mg/kg, i.p. once] a significant increase in the number of peripheral blood leukocytes and a rise in the serum of colony stimulating factor (CSF) were also confirmed. The types of CSF in serum were M-CSF and other hematopoietic growth factors, which were confirmed using McAb of IL-3, GM-CSF and PcAb of M-CSF. These beneficial effects of swerchirin on hematopoiesis may be related to its activity inducing CSFs and other hematopoietic growth factors, and warrant further evaluation of its usefulness.
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Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, P. R. China.
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D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003037:Cobalt Radioisotopes; D003115:Colony-Stimulating Factors / Q000097:blood / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D007958:Leukocyte Count / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal / Q000737:chemistry; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D014966:Xanthenes / Q000494:pharmacology*; D044004:Xanthones
D003037:Cobalt Radioisotopes; D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins; D014966:Xanthenes; D044004:Xanthones; C034385:swerchirin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor