Evaluation in mice of some iodinated melanoma imaging agents using cryosectioning and multi-wire proportional counting.
AMBIS 4000, a multi-wire proportional counter, was calibrated for iodine-125 measurements. The detector displayed a linear response over a wide dynamic range. Using whole-body mice cryosections, a linear relationship could be established between count rate per area (cpm/mm2) measured with the AMBIS 4000 detector and the count rate per gram (dpm/g) determined with an NaI(Tl) scintillation detector. A calibration curve could, thus be constructed. This new method allowed direct visual and quantitative evaluation of the biodistribution of a short series of 125I-labelled benzamides in melanoma-bearing mice. All the compounds studied showed good tumoral targeting ability. For one of them, ortho-N-(2-diethylaminoethyl)-4-iodobenzamide, liver and lung uptake decreased rapidly after dosing, making it a suitable tracer for scintigraphic detection of malignant melanoma.
Labarre
P
P
INSERM U484, Clermont-Ferrand, France.
Papon
J
J
Moreau
M F
MF
Moins
N
N
Veyre
A
A
Madelmont
J C
JC
D000818:Animals; D001549:Benzamides; D016616:Cryoultramicrotomy / Q000379:methods; D007457:Iodine Radioisotopes; D008297:Male; D008546:Melanoma, Experimental / Q000000981:diagnostic imaging*; D051379:Mice; D008810:Mice, Inbred C57BL; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D012588:Scintillation Counting / Q000295:instrumentation; D012878:Skin Neoplasms / Q000000981:diagnostic imaging*; D014018:Tissue Distribution
D001549:Benzamides; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals