Low affinity Fc gamma receptors on murine macrophages: mitogen-activated protein kinase activation and AP-1 DNA binding activity.
Mouse macrophage cell lines such as J774 express Fc receptors for IgG2a immune complexes, which upon binding of the proper ligand, trigger several signal transduction pathways. A surface to nucleus signaling through these receptors has been demonstrated. We describe here the activation of the mitogen-activated protein kinase (MAPK) and an increase in the binding of the activator protein 1 (AP-1) to DNA upon receptor stimulation. The described effects are only partially blocked by inhibitors of the Ca2+/diacylglycerol-dependent protein kinase (PKC), suggesting that differential signaling pathways are activated upon receptor cross-linking and that they converge at or above the MAPK level. These results pave the way to our understanding of Fc gammaR cross-linking induced gene expression regulation.
Cote-Vélez
M J
MJ
Departamento de Genética y Biología Molecular Centro de Investagacion y de Estudios Avanzados del IPN 2508, C.P. México, Mexico.
Ortega
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Ortega
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D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004789:Enzyme Activation; D007074:Immunoglobulin G / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D017452:Receptors, IgG / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D007074:Immunoglobulin G; D017452:Receptors, IgG; D018808:Transcription Factor AP-1; D004247:DNA; D017871:Calcium-Calmodulin-Dependent Protein Kinases