Detection and analysis of translation elongation factor 3 genes from various yeasts.
Yeast translation requires a unique elongation factor, EF-3. However, information about EF-3 genes has been limited to only a few yeast species. Here, we developed a PCR-based system to detect the EF-3 genes specifically, and identified EF-3 gene fragments from various yeast species in which EF-3 genes have not yet been found.
Uritani
M
M
Department of Chemistry, Faculty of Science, Shizuoka University, Japan. scmurit@ipc.shizuoka.ac.jp
Shoumura
Y
Y
Yamada
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D005656:Fungal Proteins; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae