A simple HPLC method for simultaneous determination of mycophenolic acid and mycophenolic acid glucuronide in plasma.
A reversed-phase high-performance liquid chromatographic method for the simultaneous determination of mycophenolic acid and its metabolite, mycophenolic acid glucuronide, is presented herein. Sample purification is limited to protein precipitation with acetonitrile. The analytes were separated on a C18 column with a mobile phase containing 30% acetonitrile and a 40 mm phosphoric acid buffer at pH 2.1 and measured with UV-detection at 215 nm.
Svensson
J O
JO
Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.
Brattström
C
C
Säwe
J
J
D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000097:blood*; D020719:Glucuronides; D006801:Humans; D015394:Molecular Structure; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000097:blood*; D013997:Time Factors
D005965:Glucuronates; D020719:Glucuronides; C113145:mycophenolic acid glucuronide; D009173:Mycophenolic Acid