title: Clinical validation of the avidin/indium-111 biotin approach for imaging infection/inflammation in orthopaedic patients.
abstract: We report here the results of a validation study of the avidin/indium-111 biotin approach in patients with skeletal lesions. This study involved 54 patients with orthopaedic conditions: 20 patients with intermediate suspected osteomyelitis of the trunk, 19 patients with infection/inflammation of prosthetic joint replacements, and 15 patients with suspected osteomyelitis of appendicular bones. Avidin (3 mg) was injected as an i.v. bolus, followed 4 h later by 111In-biotin; imaging was acquired 30 min and 16-18 h after administration of 111In-biotin. Technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO)-labelled leucocyte scintigraphy was performed in 39/54 patients. The overall sensitivity of the avidin/111In-biotin scan was 97.7% (versus 88.9% for 99mTc-HMPAO leucocyte scintigraphy). While the diagnostic performance of avidin/111In-biotin scintigraphy was similar to that of 99mTc-HMPAO leucocyte scintigraphy in patients with prosthetic joint replacements or osteomyelitis of appendicular bones, the avidin/111In-biotin approach clearly performed better than 99mTc-HMPAO leucocyte scintigraphy in patients with suspected osteomyelitis of the trunk (100% sensitivity, specificity and accuracy versus 50% sensitivity, 100% specificity and 66.7% accuracy for 99mTc-HMPAO-leucocyte scintigraphy). These results demonstrate the feasibility of the avidin/111In-biotin approach for imaging sites of infection/inflammation in the clinical setting. Although no systematic advantages of avidin/111In-biotin scintigraphy were found versus 99mTc-HMPAO leucocyte scintigraphy, the newer scintigraphic method is more practicable and involves lower biological risk for the operators.
authors.affiliation: Regional Centre of Nuclear Medicine, University of Pisa, Pisa, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Lazzeri
authors.forename: M
authors.initials: M
authors.lastname: Manca
authors.forename: N
authors.initials: N
authors.lastname: Molea
authors.forename: S
authors.initials: S
authors.lastname: Marchetti
authors.forename: V
authors.initials: V
authors.lastname: Consoli
authors.forename: L
authors.initials: L
authors.lastname: Bodei
authors.forename: R
authors.initials: R
authors.lastname: Bianchi
authors.forename: M
authors.initials: M
authors.lastname: Chinol
authors.forename: G
authors.initials: G
authors.lastname: Paganelli
authors.forename: G
authors.initials: G
authors.lastname: Mariani
mesh_terms: D001360:Avidin; D001710:Biotin; D015299:Discitis / Q000000981:diagnostic imaging; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D010019:Osteomyelitis / Q000000981:diagnostic imaging*; D011237:Predictive Value of Tests; D016459:Prosthesis-Related Infections / Q000000981:diagnostic imaging*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019690:Technetium Tc 99m Exametazime
chemical_list: D007205:Indium Radioisotopes; D019275:Radiopharmaceuticals; D001360:Avidin; D019690:Technetium Tc 99m Exametazime; D001710:Biotin