Acrylamide causes preimplantation abnormalities in embryos and induces chromatin-adducts in male germ cells of mice.
Acrylamide, a known male postmeiotic germ cell mutagen, caused a dose-dependent increase in the frequency of morphologic abnormalities in preimplantation embryos. Single-cell eggs, growth retardation, and blastomere lysis were detected after paternal treatment with acrylamide (10 to 50 mg/kg, 5 d). The major effects were seen at weeks 1 to 3 after male treatment, with the highest level of abnormalities at the first week (> 90% vs. 5% in control). The frequency of abnormal four-day embryos was similar to preimplantation loss assessed at 15 to 16 d p.c. A > 100-fold elevation of chromatin adducts in sperm was observed during 1st and 2nd week after treatment, after which adduct levels decreased to baseline level. However, morphologic defects in embryos are not fully explained by the spermatid adduct curve. These findings demonstrate the effects of paternal exposure to acrylamide on preimplantation development and indicate a potential risk to the offspring of men exposed to acrylamide.
Holland
N
N
Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720, USA.
Ahlborn
T
T
Turteltaub
K
K
Markee
C
C
Moore
D
D
Wyrobek
A J
AJ
Smith
M T
MT
D020106:Acrylamide / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005854:Germ Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009152:Mutagenicity Tests; D013091:Spermatogenesis / Q000187:drug effects; D013723:Teratogens / Q000633:toxicity*; D013997:Time Factors
D002843:Chromatin; D013723:Teratogens; D020106:Acrylamide