[Immunohistochemical detection of cytokine receptors on cryostat tissue sections].
We have studied tissue expression of the cytokine receptors using a high sensitivity biotin-streptavidin system on cryostat sections. We used a panel of monoclonal antibodies from the 6th International Workshop on Human Leukocyte Differentiation Antigens, namely CD25 (IL-2R alpha), CD95 (FAS antigen), CD116 (GM CSFR), CD117 (SCFR), CD120 alpha (TNFR I), CD120b (TNFR II), CD121a (IL-1R I), CDw123 (IL-3R), CD124 (IL-4R), CD126 (IL-6R), CD127 (IL-7R), CDw128 (IL-8R), CD130 (gpl130), CD131 (IL-3R), CD132 (IL-2R gamma), CD134 (OC-40), CD135 (FLT3/FLK2). Examined tissues (lymph nodes and spleens) were obtained from 12 patients with folicular non-Hodgkin's lymphoma, periferal T non-Hodgkin's lymphoma, B lymphoma, myeloma, Hodgkin's disease, two cases of T cell rich B-lymphoma, autoimmune haemolytic anemia and two cases of rudimentary trombocytopenic purpura. Our results indicate that immunohistological technology using native tissues on cryostat sections, monoclonal antibodies and the visualisation with biotin-streptavidin is a particularly suitable supplementary staining procedure for detection of the cytokine receptors in tissues.
Kumberová
A
A
Ustav hematologie a krevní transfuze, Praha.
Jirásek
A
A
Koubek
K
K
Kolárová
M
M
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D005260:Female; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D018121:Receptors, Cytokine / Q000032:analysis*; D013154:Spleen / Q000737:chemistry
D000911:Antibodies, Monoclonal; D018121:Receptors, Cytokine
Imunohistochemická detekce cytokinových receptorů na kryostatových tkánových rezech.