Effects of alimentary intact proteins and their oligopeptide hydrolysate on growth, nitrogen retention, and small bowel adaptation in inflammatory turpentine rat.
The effects of dietary proteins given as whole proteins (WP) or as a peptide hydrolysate (PH) on growth, nitrogen retention, and small bowel adaptation were assessed using two groups of male Wistar rats. Measurements were made 18, 42, and 66 h after acute inflammation induced by subcutaneous injections of 0.125 mL turpentine and in two control groups (n = 12). The two diets had the same caloric, nitrogen, vitamin, and mineral content. The WP diet resulted in better weight gain, nitrogen retention, and small intestinal adaptation by control rats than did the PH diet. Loss of body weight after 18 h of acute inflammation was significantly lower and nitrogen retention significantly higher in animals on the WP diet than in those on the PH diet. Small intestine morphology was maintained with the WP diet, whereas villus height was significantly lower after 66 h, and there were fewer mitoses per crypt in the rats on the PH diet. Glucoamylase activity at all times, and N-aminopeptidase activity at 18 h, were significantly higher in rats on the WP diet. The putrescine (at 42 h) and spermidine (at 18 h) concentrations in the mucosa were higher in the rats on the WP diet. These data suggest that synthetic diets should be tested for their nutritional value during acute inflammation before they are used in human nutrition.
Zarrabian
S
S
INSERM U458, Robert Debrè Hospital, Paris, France.
Buts
J P
JP
Fromont
G
G
Tran
T U
TU
Macry
J
J
Mendy
F
F
Roger
L
L
Cèzard
J P
JP
D000222:Adaptation, Physiological; D000818:Animals; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology*; D004751:Enteritis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D006128:Growth / Q000187:drug effects*; D006868:Hydrolysis; D007413:Intestinal Mucosa / Q000473:pathology / Q000503:physiopathology; D007421:Intestine, Small / Q000473:pathology / Q000503:physiopathology*; D007583:Jejunum / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D009929:Organ Size; D011073:Polyamines / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014425:Turpentine; D015431:Weight Loss
D004044:Dietary Proteins; D009842:Oligopeptides; D011073:Polyamines; D009584:Nitrogen; D014425:Turpentine