Relationships between bone mineral density, serum vitamin D metabolites and calcium:phosphorus intake in healthy perimenopausal women.
OBJECTIVE
To determine the relationships between serum vitamin D metabolites, bone mass, and dietary calcium and phosphorus in a cohort of 510 healthy Danish perimenopausal women.


METHODS
A population-based cross-sectional study.


METHODS
A total of 510 healthy women aged 45-58 years, with amenorrhoea for 3-24 months. None of the women was using hormone replacement therapy.


METHODS
Measurements of total bone mineral content and regional bone mineral density were performed by dual-energy X-ray absorptiometry. Analyses of serum levels of 25-OHD and 1,25-(OH)2D, intact PTH, ionized calcium and phosphate, as well as biochemical markers of bone turnover in blood and urine. Assessment of calcium and phosphorus intake using dietary records.


RESULTS
A consistent inverse relationship between serum 1,25-(OH)2D and bone mineral content/ density was found in whole-body mineral content (P = 0.001), spine (P = 0.005) and femoral neck (P<0.05). There was a positive relationship between levels of 1,25-(OH)2D and biochemical bone markers, indicating that high levels of 1,25-(OH)2D are accompanied by increased bone turnover. The dietary calcium:phosphorus ratio was inversely related to serum 1,25-(OH)2D (P = 0.04) and positively related to bone mineral density (P<0.0005). No relationships could be detected between levels of PTH, serum ionized calcium and phosphate, and serum vitamin D metabolites.


CONCLUSIONS
Within normal physiological range, elevated levels of 1,25-(OH)2D were associated with decreased bone mineral density and content, reduced calcium:phosphorus ratio in the diet and increased bone turnover.
Brot
C
C
Osteoporosis Research Centre, Department of Rheumatology, Copenhagen Municipal Hospital, Denmark.
Jørgensen
N
N
Madsen
O R
OR
Jensen
L B
LB
Sørensen
O H
OH
D015502:Absorptiometry, Photon; D015519:Bone Density; D002136:Calcium, Dietary / Q000008:administration & dosage* / Q000097:blood; D003430:Cross-Sectional Studies; D003718:Denmark; D015930:Diet Records; D005260:Female; D006801:Humans; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood; D016226:Phosphorus, Dietary / Q000008:administration & dosage* / Q000097:blood; D017697:Premenopause; D012016:Reference Values; D014807:Vitamin D / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood*
D002136:Calcium, Dietary; D010281:Parathyroid Hormone; D016226:Phosphorus, Dietary; D014807:Vitamin D; C097949:1,25-dihydroxyvitamin D; C104450:25-hydroxyvitamin D