Structure of the Escherichia coli fumarate reductase respiratory complex.
The integral membrane protein fumarate reductase catalyzes the final step of anaerobic respiration when fumarate is the terminal electron acceptor. The homologous enzyme succinate dehydrogenase also plays a prominent role in cellular energetics as a member of the Krebs cycle and as complex II of the aerobic respiratory chain. Fumarate reductase consists of four subunits that contain a covalently linked flavin adenine dinucleotide, three different iron-sulfur clusters, and at least two quinones. The crystal structure of intact fumarate reductase has been solved at 3.3 angstrom resolution and demonstrates that the cofactors are arranged in a nearly linear manner from the membrane-bound quinone to the active site flavin. Although fumarate reductase is not associated with any proton-pumping function, the two quinones are positioned on opposite sides of the membrane in an arrangement similar to that of the Q-cycle organization observed for cytochrome bc1.
Iverson
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Graduate Option in Biochemistry, 147-75CH, California Institute of Technology, Pasadena, CA 91125, USA.
Luna-Chavez
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Cecchini
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Rees
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D000332:Aerobiosis; D000693:Anaerobiosis; D001665:Binding Sites; D002462:Cell Membrane / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D004579:Electron Transport; D004734:Energy Metabolism; D004926:Escherichia coli / Q000201:enzymology*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011487:Protein Conformation; D017510:Protein Folding; D011809:Quinones / Q000737:chemistry / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism
D005650:Fumarates; D007506:Iron-Sulfur Proteins; D011809:Quinones; D005182:Flavin-Adenine Dinucleotide; C032005:fumaric acid; D013385:Succinate Dehydrogenase