title: IgG, IgG1 and IgM response in Trichinella spiralis-infected mice treated with 4-deoxypirydoxine or fed a Vitamin B6-deficient diet.
abstract: The aim of this study was to investigate the effect of pyridoxine (Vitamin B6) deficiency on the immunological response of BALB/c mice infected with the parasite T. spiralis. Specific anti-parasite IgM and IgG immunoglobulins were detected by ELISA method in the serum of treated animals at different periods for 60 days post infection. Vitamin B6-deficiency was induced in two separate groups of mice by either (1) maintaining the mice on a Vitamin B6-deficient synthetic pellet diet for 40 days before infection, or (2) by daily intraperitoneal injection of 8 x 10(5) M/100 microl of 4-Deoxypyridoxine (4-DPD), a potent antagonist of Vitamin B6 for 20 days prior to infection. These two groups of mice were then injected with 100 larvae (L1-T. spiralis) per os. Parasite burdens in the mice were observed by light microscopy. Cysts were present in the diaphragms of the mice after 60 days post-infection. Parasite specific IgG, as well as IgG1 levels were determined in the sera of infected mice fed a normal diet. These levels were found to be lower in the 4-DPD-treated mice compared to the untreated mice. The inhibition started from the 10th day and continued to the 60th day, and in the 4-DPD-treated group the inhibition initiated after 24 h to 60 days. IgM level also was depressed by 4-DPD, starting from 24 h after injection of the compound. In mice fed Vitamin B6-deficient diets the levels of IgG were lower than in mice fed normal diets. These results show that BALB/c mice infected with T. spiralis and fed either a Vitamin B6-deficient diet or a diet which included the Vitamin B6-antagonist, 4-DPD, both influence the course of IgG, IgG1 and IgM production.
authors.affiliation: Department of Parasitology, Veterinary Faculty, Aristotle University, Thessaloniki, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Frydas
authors.forename: M
authors.initials: M
authors.lastname: Reale
authors.forename: D
authors.initials: D
authors.lastname: Vacalis
authors.forename: R C
authors.initials: RC
authors.lastname: Barbacane
authors.forename: F C
authors.initials: FC
authors.lastname: Placido
authors.forename: I
authors.initials: I
authors.lastname: Cataldo
authors.forename: M
authors.initials: M
authors.lastname: Di Gioacchino
authors.forename: E
authors.initials: E
authors.lastname: Karagouni
authors.forename: E
authors.initials: E
authors.lastname: Dotsika
authors.forename: G
authors.initials: G
authors.lastname: Anogiannakis
authors.forename: A
authors.initials: A
authors.lastname: Trakatellis
authors.forename: P
authors.initials: P
authors.lastname: Conti
mesh_terms: D000818:Animals; D004032:Diet; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011736:Pyridoxine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D017160:Trichinella spiralis / Q000302:isolation & purification*; D014235:Trichinellosis / Q000276:immunology*; D026681:Vitamin B 6 Deficiency / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; C004675:4-deoxypyridoxine; D011736:Pyridoxine