title: A lobster phospholipase C-beta that associates with G-proteins in response to odorants.
abstract: A cDNA clone encoding a protein of 1116 amino acids with significant homology to beta-isoforms of phospholipase C was isolated from lobster olfactory organ cDNA libraries and named lobPLCbeta. This cDNA hybridized predominantly to a 9 kb transcript in RNA from olfactory organ, pereiopod, brain, and eye-eyestalk and to several smaller minor transcripts only in eye-eyestalk. An antiserum raised to the C terminus of lobPLCbeta detected immunoreactivity in a single 130 kDa band in olfactory aesthetasc hairs, olfactory organ, pereiopod, dactyl, and brain. In eye-eyestalk this 130 kDa band was abundant, and minor bands of 100, 79, and 57 kDa also were detected. In cross sections of the aesthetasc hairs, immunoreactivity was detected in the outer dendritic segments of the olfactory receptor neurons, the site of olfactory transduction. A complex odorant caused lobPLCbeta immunoreactivity to increase in membrane fractions and decrease in soluble fractions of homogenates of aesthetasc hairs. The odorant also increased the amount of lobPLCbeta in immunoprecipitates of Galphaq and Gbeta from homogenates of aesthetasc hairs. These results support the conclusion that lobPLCbeta mediates olfactory transduction.
authors.affiliation: Department of Physiology, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298, USA.
authors.forename: F
authors.initials: F
authors.lastname: Xu
authors.forename: T S
authors.initials: TS
authors.lastname: McClintock
mesh_terms: D000825:Animal Structures / Q000201:enzymology; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003712:Dendrites / Q000201:enzymology; D005136:Eye Proteins / Q000032:analysis; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D044387:GTP-Binding Protein beta Subunits; D044388:GTP-Binding Protein gamma Subunits; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D020962:Heterotrimeric GTP-Binding Proteins; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008121:Nephropidae; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000201:enzymology* / Q000648:ultrastructure; D054799:Phospholipase C beta; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D012903:Smell / Q000502:physiology*; D010738:Type C Phospholipases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D005136:Eye Proteins; C118981:G-protein Beta gamma; D044387:GTP-Binding Protein beta Subunits; D044388:GTP-Binding Protein gamma Subunits; D007527:Isoenzymes; D012333:RNA, Messenger; D015544:Inositol 1,4,5-Trisphosphate; D010738:Type C Phospholipases; D054799:Phospholipase C beta; D019204:GTP-Binding Proteins; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins