Migration inhibitory factor related protein-8 (MRP-8) is an autocrine chemotactic factor for periodontal ligament cells.
Previous studies have suggested that human periodontal ligament (PDL) cells secrete a chemotactic factor which stimulates motility in an autocrine manner. Here we report the partial amino acid sequence of a purified factor which shows 100% homology with human migration inhibitory factor related protein-8 (MRP-8). In addition, reverse-transcription polymerase chain-reaction (RT-PCR) analysis revealed that mRNA encoding MRP-8 was expressed in cultured human PDL cells. To confirm that MRP-8 is chemotactic for PDL cells, we synthesized 25 mer peptides overlapped by 5 amino acids covering the entire MRP-8 protein and tested them for their chemotactic activities. The data indicated that amino acid residues 21-45 showed chemotactic activity for cultured human PDL cells. The maximum chemotactic response was observed at the concentration of 10(-15) mol/mL for human PDL cells. The chemotactic activity was estimated to be approximately 1000-fold higher than that of platelet-derived growth factor (PDGF), insulin-like growth factors-I and -II (IGF-I, -II), and epidermal growth factor (EGF) when compared on a molar basis. Since MRP-8 is reported to be produced mainly by neutrophils and monocytes, the result of the current study may suggest another important role of MRP-8 in human PDL cells.
Nishimura
F
F
Department of Periodontology and Endodontology, Okayama University Dental School, Japan.
Terranova
V P
VP
Sawa
T
T
Murayama
Y
Y
D000595:Amino Acid Sequence; D000943:Antigens, Differentiation / Q000032:analysis* / Q000235:genetics / Q000494:pharmacology; D019898:Autocrine Communication; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000494:pharmacology; D040501:Calgranulin A; D002465:Cell Movement; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000032:analysis* / Q000235:genetics / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis* / Q000235:genetics / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
D000943:Antigens, Differentiation; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D002630:Chemotactic Factors; D008263:Macrophage Migration-Inhibitory Factors; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II