title: Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast.
abstract: Telomeric TG-rich repeats and their associated proteins protect the termini of eukaryotic chromosomes from end-to-end fusions. Associated with the cap structure at yeast telomeres is a subtelomeric domain of heterochromatin, containing the silent information regulator (SIR) complex. The Ku70/80 heterodimer (yKu) is associated both with the chromosome end and with subtelomeric chromatin. Surprisingly, both yKu and the chromatin-associated Rap1 and SIR proteins are released from telomeres in a RAD9-dependent response to DNA damage. yKu is recruited rapidly to double-strand cuts, while low levels of SIR proteins are detected near cleavage sites at later time points. Consistently, yKu- or SIR-deficient strains are hypersensitive to DNA-damaging agents. The release of yKu from telomeric chromatin may allow efficient scanning of the genome for DNA strand breaks.
authors.affiliation: Swiss Institute for Experimental Cancer Research, Epalinges.
authors.forename: S G
authors.initials: SG
authors.lastname: Martin
authors.forename: T
authors.initials: T
authors.lastname: Laroche
authors.forename: N
authors.initials: N
authors.lastname: Suka
authors.forename: M
authors.initials: M
authors.lastname: Grunstein
authors.forename: S M
authors.initials: SM
authors.lastname: Gasser
mesh_terms: D034961:Antigens, Nuclear; D001761:Bleomycin / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000378:metabolism; D004249:DNA Damage; D004265:DNA Helicases; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis; D019281:Dimerization; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D005838:Genotype; D000072200:Ku Autoantigen; D009687:Nuclear Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D016615:Telomere / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D020668:rap GTP-Binding Proteins
chemical_list: D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; C061573:SIR4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D014157:Transcription Factors; C081647:high affinity DNA-binding factor, S cerevisiae; D001761:Bleomycin; C069930:rad9 protein; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific; D019204:GTP-Binding Proteins; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D020668:rap GTP-Binding Proteins; D000072200:Ku Autoantigen