[Construction of expressing vector for phage display scFv and a mouse unspecified antibody library].
The display of antibody gene library on the surface of E. coli filamentous phage offers a new method of obtaining antibodies against specific antigens. In the paper, it is reported that a phagemid for phage display antibody, named after pFUW80, characterized expressing scFv either secretly or stickily. With a series of designed PCR primers, heavy-chain and light-chain variable region genes of mice antibodies were amplified and a mouse unspecified single-chain antibody library with the size of 1.2 x 10(6) clones was constructed. From this library, phage was selected out against antigen human IgG and detected by ELISA and partial sequence analysis. These primary results established the foundation for future research using this system.
Zhang
W G
WG
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Liu
X F
XF
Wang
Y
Y
Jian
X
X
Lin
Q
Q
Chen
A
A
Wu
C W
CW
Huang
H M
HM
Li
Z H
ZH
Zhang
Y
Y
Huang
H L
HL
D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv