title: [Trichome staining method applied to stools from HIV-infected patients with diarrhea, for microsporidia investigation].
abstract: The microsporidia have been involved in several clinical manifestations in patients with AIDS, of whom diarrhoea is the commonest. The diagnosis of microsporidiasis depended on invasive procedures and the identification of the organisms is made by electron microscopy. The modified trichrome staining method allows that the diagnosis be made without such procedures by using light microscopy. In the present work, the modified trchrome method was applied in stools from 62 patients with diarrhoea, who had asymptomatic HIV infection or AIDS. Of the 62 samples analyzed, there was detection of microsporidial spores in one. This work confirms the existence of such protozoans in our patients, associated with manifestations of chronic diarrhoea in patients with AIDS who have severe immunodeficiency and ascertains that this staining method allows satisfactory identification of microsporidia from faeces, as well points out some directions to further studies.
authors.affiliation: Departamento de Doenças Infecciosas e Parasitárias, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, Brasil.
authors.forename: J G
authors.initials: JG
authors.lastname: Amato
authors.forename: V S
authors.initials: VS
authors.lastname: Amato
authors.forename: V
authors.initials: V
authors.lastname: Amato Neto
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000469:parasitology*; D000293:Adolescent; D000328:Adult; D000818:Animals; D001391:Azo Compounds; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D003967:Diarrhea / Q000469:parasitology*; D004801:Eosine Yellowish-(YS); D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008739:Methyl Green; D016816:Microsporida / Q000302:isolation & purification*; D008875:Middle Aged; D013194:Staining and Labeling / Q000379:methods
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)
vernacular_title: Aplicação de método de coloração tricrômica, em fezes diarréicas de infectados pelo HIV, para pesquisa de microsporídios.