[Interactions between steroidogenic cells and capillary endothelial cells in the adrenal gland].
Adrenocortical capillary endothelial (ACE) cells showed a two- to three-fold increase in number when they were cocultured with bovine adrenocortical (BAC) cells from the zona fasciculata-reticularis. This effect was detectable within 48 h, persisted throughout the seven-day coculture period, and occurred in the absence of addition of exogenous growth factors. It was similar to that obtained by addition of 1 ng/ml of FGF-2. Adrenal cortex tumor cells from humans (NCI) and mice (Y1) also stimulated ACE cell growth, whereas NIH 3T3 cells did not, suggesting an effect specific of steroid-producing cells. Addition of an FGF-2-neutralizing antibody failed to inhibit the BAC cell-induced ACE cell growth stimulation, indicating that FGF-2 was not involved. BAC cell-conditioned medium stimulated ACE cell growth, indicating a role for a diffusible factor. When BAC cells were cocultured with ACE cells in a 3D collagen gel, capillary-like tubes developed, consistent with secretion by BAC cells of angiogenic factors. Studies are under way to identify these factors.
Gaillard
I
I
Unité INSERM 244, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, Grenoble, France.
Prugnard
E
E
Brand
C
C
Chambaz
E
E
Vilgrain
I
I
D016475:3T3 Cells; D000302:Adrenal Cortex / Q000166:cytology* / Q000187:drug effects; D000306:Adrenal Cortex Neoplasms / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002417:Cattle; D002450:Cell Communication; D002455:Cell Division; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D051379:Mice; D014407:Tumor Cells, Cultured
D016222:Fibroblast Growth Factor 2
Intéractions entre les cellules stéroïdogènes et les cellules endothéliales de capillaires dans la glande surrénale.