Alpha1,3-fucosyltransferase IX (Fuc-TIX) is very highly conserved between human and mouse; molecular cloning, characterization and tissue distribution of human Fuc-TIX.
The amino acid sequence of Fuc-TIX is very highly conserved between mouse and human. The number of non-synonymous nucleotide substitutions of the Fuc-TIX gene between human and mouse was strikingly low, and almost equivalent to that of the alpha-actin gene. This indicates that Fuc-TIX is under a strong selective pressure of preservation during evolution. The human Fuc-TIX (hFuc-TIX) showed a unique characteristics, i.e. hFuc-TIX was not activated by Mn2+ and Co2+, whereas hFuc-TIV and hFuc-TVI were activated by the cations. The hFuc-TIX transcripts were abundantly expressed in brain and stomach, and interestingly were detected in spleen and peripheral blood leukocytes.
Kaneko
M
M
Division of Cell Biology, Institute of Life Science, Soka University, Tokyo, Japan.
Kudo
T
T
Iwasaki
H
H
Ikehara
Y
Y
Nishihara
S
S
Nakagawa
S
S
Sasaki
K
K
Shiina
T
T
Inoko
H
H
Saitou
N
N
Narimatsu
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D005647:Fucosyltransferases / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D015723:Gene Library; D014644:Genetic Variation; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase