Regulation of the UNC-18-Caenorhabditis elegans syntaxin complex by UNC-13.
The Caenorhabditis elegans unc-13, unc-18, and unc-64 genes are required for normal synaptic transmission. The UNC-18 protein binds to the unc-64 gene product C. elegans syntaxin (Ce syntaxin). However, it is not clear how this protein complex is regulated. We show that UNC-13 transiently interacts with the UNC-18-Ce syntaxin complex, resulting in rapid displacement of UNC-18 from the complex. Genetic and biochemical evidence is presented that UNC-13 contributes to the modulation of the interaction between UNC-18 and Ce syntaxin.
Sassa
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Department of Nutrition, School of Medicine, Tokushima University, Tokushima 770-0042, Japan.
Harada
S
S
Ogawa
H
H
Rand
J B
JB
Maruyama
I N
IN
Hosono
R
R
D000483:Alleles; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D005089:Exocytosis / Q000502:physiology; D017353:Gene Deletion; D015870:Gene Expression / Q000502:physiology; D017238:Genes, Helminth / Q000502:physiology; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D016254:Mutagenesis, Insertional / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism; D010641:Phenotype; D010750:Phosphoproteins; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D009435:Synaptic Transmission / Q000235:genetics; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins
D000954:Antigens, Surface; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D050827:Syntaxin 1; C082636:Unc-18 protein, C elegans; D033921:Vesicular Transport Proteins; C083141:phorbol ester binding protein; C494215:unc-64 protein, C elegans