CP-10, a chemotactic peptide, is expressed in lesions of experimental autoimmune encephalomyelitis, neuritis, uveitis and in C6 gliomas.
CP-10 (chemotactic protein of m.w. 10,000) is a member of the S100 superfamily of Ca2+ binding peptides, which has potent chemotactic activity for murine and human myeloid cells. Here we report on the generation of monoclonal antibodies against CP-10 and accumulation of CP-10+ cells during experimental autoimmune encephalomyelitis (EAE), neuritis (EAN), uveitis (EAU) and in experimentally transplanted C6 gliomas. During acute inflammation, CP-10 is mainly expressed by large ED1+ monocytic perivascular cells that accumulate at days 11-14. CP-10+ cells are predominantly located in areas of cellular infiltration but are as well found in the meninges and infiltrating the brain parenchyma. In transplanted gliomas, CP-10+ cells are located exclusively within the tumor parenchyma. Using double labeling experiments, other cells participating in the inflammatory reaction were found to express CP-10, like few lymphoblastic W3/13+ cells in the vicinity of the inflammatory infiltrate.
Deininger
M H
MH
Institute of Brain Research, University of Tübingen, Germany. hirnforschung@uni-tuebingen.de
Zhao
Y
Y
Schluesener
H J
HJ
D000818:Animals; D000911:Antibodies, Monoclonal; D001327:Autoimmune Diseases / Q000276:immunology; D001483:Base Sequence; D040501:Calgranulin A; D002630:Chemotactic Factors / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D017931:DNA Primers; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D005910:Glioma; D006825:Hybridomas; D007962:Leukocytes / Q000737:chemistry / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009443:Neuritis / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000276:immunology; D009418:S100 Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured / Q000276:immunology; D014605:Uveitis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D040501:Calgranulin A; D002630:Chemotactic Factors; D017931:DNA Primers; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins; D009418:S100 Proteins; C086145:S100a8 protein, mouse