Stimulation of motility of human renal cell carcinoma by SPARC/Osteonectin/BM-40 associated with type IV collagen.
SPARC is known to be important in development and tissue remodelling. Here, we examined the effects of SPARC (secreted protein, acidic and rich in cysteine; osteonectin) derived from a rat osteosarcoma cell line on migration of renal cell carcinoma (RCC) by a Boyden chamber assay. YCR RCC cells migrated through type IV collagen-coated filters without stimuli (basal level). SPARC in the lower compartment stimulated chemotactic activity to 120% of the basal level, whereas premixing of YCR with purified SPARC before inoculation reduced their migration to 72% of the basal level. Furthermore, SPARC mixed with type IV collagen more efficiently stimulated their migration in a concentration-dependent manner (up to 170% of the basal level). This suggests that SPARC bound to type IV collagen plays a role in tumor invasion.
Kato
Y
Y
Department of Oral Biochemistry, Kanagawa Dental College, Yokosuka, Japan.
Sakai
N
N
Baba
M
M
Kaneko
S
S
Kondo
K
K
Kubota
Y
Y
Yao
M
M
Shuin
T
T
Saito
S
S
Koshika
S
S
Kawase
T
T
Miyagi
Y
Y
Aoki
I
I
Nagashima
Y
Y
D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D015676:Osteonectin / Q000302:isolation & purification / Q000494:pharmacology*; D012516:Osteosarcoma / Q000473:pathology; D051381:Rats; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects
D017077:Culture Media, Conditioned; D015676:Osteonectin; D003094:Collagen