Experimental inoculation of heifers with bovine adenovirus type 3.
Nine 2-year-old heifers having BAd3-neutralizing antibody titers between 1:120 and 1:1080 were individually exposed intranasally to an aerosol of 10(8) pfu of wild type (wt) bovine adenovirus type 3 (BAd3). Four animals were kept as non-inoculated controls. The heifers were examined daily for rectal temperature, weight gain/loss, nasal and ocular discharges, and other clinical signs for 10 d post-inoculation. None of the animals showed any sign of clinical disease. Virus excretion was observed in one animal only on Day 3 post-inoculation. All BAd3-inoculated heifers demonstrated a significant (P < 0.005, paired t-test) rise in BAd3-specific serum IgG, IgG1, or IgG2 ELISA titers and virus-neutralizing antibody titers compared to the titers before inoculation. All virus-inoculated animals demonstrated increased levels of BAd3-specific IgA ELISA titers in nasal secretions. These results suggest that in the presence of circulating BAd3-neutralizing antibodies, intranasal inoculation of cattle with wt BAd3 would result in inapparent infection.
Mittal
S K
SK
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907-1243, USA. skmittal@vet.purdue.edu
Tikoo
S K
SK
Van Donkersgoed
J
J
Beskorwayne
T
T
Godson
D L
DL
Babiuk
L A
LA
D000257:Adenoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D017921:Mastadenovirus / Q000276:immunology*; D009500:Neutralization Tests; D014765:Viral Vaccines / Q000009:adverse effects
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines