title: The pvc gene cluster of Pseudomonas aeruginosa: role in synthesis of the pyoverdine chromophore and regulation by PtxR and PvdS.
abstract: A putative operon of four genes implicated in the synthesis of the chromophore moiety of the Pseudomonas aeruginosa siderophore pyoverdine, dubbed pvcABCD (where pvc stands for pyoverdine chromophore), was cloned and sequenced. Mutational inactivation of the pvc genes abrogated pyoverdine biosynthesis, consistent with their involvement in the biosynthesis of this siderophore. pvcABCD expression was negatively regulated by iron and positively regulated by both PvdS, the alternate sigma factor required for pyoverdine biosynthesis, and PtxR, a LysR family activator previously implicated in exotoxin A regulation.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
authors.forename: A
authors.initials: A
authors.lastname: Stintzi
authors.forename: Z
authors.initials: Z
authors.lastname: Johnson
authors.forename: M
authors.initials: M
authors.lastname: Stonehouse
authors.forename: U
authors.initials: U
authors.lastname: Ochsner
authors.forename: J M
authors.initials: JM
authors.lastname: Meyer
authors.forename: M L
authors.initials: ML
authors.lastname: Vasil
authors.forename: K
authors.initials: K
authors.lastname: Poole
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009842:Oligopeptides; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; C093593:PvdS protein, Pseudomonas aeruginosa; D012808:Sigma Factor; D014157:Transcription Factors; C042453:pyoverdin; C073470:LysR protein, Bacteria