title: Nucleotide sequence of the streptococcin A-FF22 lantibiotic regulon: model for production of the lantibiotic SA-FF22 by strains of Streptococcus pyogenes.
abstract: Streptococcin A-FF22 (SA-FF22) is a type AII linear lantibiotic produced by Streptococcus pyogenes strain FF22. Sequence analysis of an approximate 10 kb region of DNA showed it to contain nine open reading frames arranged in three operons responsible for regulation, biosynthesis and immunity of SA-FF22. This region is organized similarly to the Lactococcus lactis lacticin 481 region, however, unlike lacticin 481, a two-component regulatory system is essential for SA-FF22 production. Located immediately downstream of the scn region is a putative transposase gene, the presence of which supports earlier data that indicated a mobile nature to this region.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: McLaughlin
authors.forename: J J
authors.initials: JJ
authors.lastname: Ferretti
authors.forename: W L
authors.initials: WL
authors.lastname: Hynes
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001430:Bacteriocins; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D018085:Regulon; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; D012329:RNA, Bacterial; C016887:A-FF22