title: Isolation and characterization of a highly sulfated heparan sulfate from ascidian test cells.
abstract: Several sulfated polysaccharides have been isolated from the test cells of the ascidian Styela plicata. The preponderant polysaccharide is a highly sulfated heparan sulfate with the following disaccharide composition: (1) UA(2SO4)-1-->4 GlcN(SO4)(6SO4), 53%; (2) UA(2SO4)-1-->4-GlcN(SO4), 22%; (3) UA-1-->4-GlcNAc(6SO4), 14% and (4) UA-1-->4-GlcN(SO4), 11%. Two others unidentified sulfated polysaccharides and a glycogen polymer are also present in the ascidian eggs. Histochemistry with the cationic dye 1,9-dimethyl-methylene blue and biochemical analysis of the 35S-sulfate incorporation into the eggs reveal that the sulfated glycans are present exclusively in the test cells. Possibly these sulfated polysaccharides are involved in important functions of these cells, such as to confer an external and hydrophilic layer which protect the eggs and the larvae of ascidians.
authors.affiliation: Laboratório de Tecido Conjuntivo, Hospital Universitário and Departamento de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ 21941-590, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Cavalcante
authors.forename: P A
authors.initials: PA
authors.lastname: Mourão
authors.forename: M S
authors.initials: MS
authors.lastname: Pavão
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006003:Glycogen / Q000032:analysis; D006497:Heparan Sulfate / Q000737:chemistry / Q000302:isolation & purification*; D006651:Histocytochemistry; D008190:Lyases; D010063:Ovum / Q000737:chemistry*; D013462:Sulfur Radioisotopes; D014561:Urochordata / Q000737:chemistry*
chemical_list: D006003:Glycogen; D006497:Heparan Sulfate; D008190:Lyases; D013462:Sulfur Radioisotopes