Theoretical aspects of single pulse induced modulation of cell-cell interactions favoring iso-electrofusion.
A single pulse-mediated electrofusion of freely suspending widely separated cells could reduce steps in the protocol. Prediction of such electrofusion is still unsolved. In pre-pulse condition, quantitative estimation with three-layered model cell surface reveals electrostatic repulsion (due to negative surface charge) is the major hindrance to membrane-to-membrane contact. On theoretical grounds we predict that designed single high voltage pulsing on widely separated model cell surface can counteract the non-specific repulsive interaction and favor approach of two apposed membranes. But hydrodynamic modulation of pulse exposed cell surface interaction can hamper approach of membranes contact when cells are held at a gap of more than approximately 450 A.
Biswas
S
S
Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi.
Guha
S K
SK
D002448:Cell Adhesion; D008962:Models, Theoretical; D055672:Static Electricity