title: Structure of a neutral O-specific polysaccharide of the bacterium Providencia alcalifaciens O5.
abstract: A neutral polysaccharide containing D-galactose, 2-acetamido-2-deoxy-D-glucose, and 3-acetamido-3,6-dideoxy-D-glucose (Qui3NAc) in the ratios 2:1:1 was obtained by mild acid degradation of lipopolysaccharide of the bacterium Providencia alcalifaciens O5 followed by gel chromatography and ion-exchange chromatography or treatment with anhydrous hydrogen fluoride. On the basis of full acid hydrolysis, methylation, and 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), H-detected heteronuclear 1H,13C single-quantum coherence (HSQC), and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established:
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: O V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: E V
authors.initials: EV
authors.lastname: Kholodkova
authors.forename: E S
authors.initials: ES
authors.lastname: Stanislavsky
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry*
chemical_list: D019081:O Antigens