title: Distribution of rantes and interleukin-5 in allergic nasal mucosa and nasal polyps.
abstract: Eosinophil-chemoattracting cytokines are thought to be important in the pathogenesis of allergic inflammation. However, little is known about the presence and significance of RANTES in nasal allergy and nasal polyps, two well-known rhinologic disorders characterized by eosinophil infiltration in the tissue. In order to evaluate the role of RANTES in eosinophil infiltration in vivo, the tissue distributions of RANTES and interleukin-5 (IL-5) and their correlation with eosinophil infiltration were investigated. Nasal mucosa specimens were obtained from 9 allergic and 12 control subjects, and nasal polyps from 6 allergic and 9 nonallergic subjects. All the subjects were divided into 4 groups: normal mucosa, allergic mucosa, nonallergic polyps, and allergic polyps. To identify the cellular localizations of RANTES and IL-5, we used specific immunohistochemical staining. We also investigated the differences in cytokine expression among the 4 groups, and the correlation between cytokine expression and eosinophil infiltration in the tissue. RANTES was expressed in the epithelium, endothelium, and some submucosal cells, while IL-5 was confined to the cells in the submucosa. Expression of both RANTES and IL-5 significantly increased in allergic mucosa and nasal polyps compared to normal mucosa; however, there was no significant difference in their expression between allergic and nonallergic polyps. Both cytokines had a significant correlation between their expression and either total or activated eosinophil numbers. The results of this study suggest that RANTES, as well as IL-5, plays a role in eosinophil recruitment in allergic nasal mucosa and nasal polyps in vivo.
authors.affiliation: Department of Otorhinolaryngology, College of Medicine, Seoul National University, Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Lee
authors.forename: C S
authors.initials: CS
authors.lastname: Rhee
authors.forename: S O
authors.initials: SO
authors.lastname: Lee
authors.forename: Y G
authors.initials: YG
authors.lastname: Min
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D018946:Chemokine CCL5 / Q000276:immunology / Q000378:metabolism*; D004727:Endothelium / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism; D005169:Factor VIII / Q000276:immunology / Q000378:metabolism; D006801:Humans; D015848:Interleukin-5 / Q000276:immunology / Q000378:metabolism*; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D009298:Nasal Polyps / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D012221:Rhinitis, Allergic, Perennial / Q000150:complications / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5; D015848:Interleukin-5; D005169:Factor VIII