Daily exercise enhances acetylcholine-induced dilation in mesenteric and hindlimb vasculature of hypertensive rats.
The effect of daily spontaneous running (DSR) on endothelial function was examined in spontaneously hypertensive rats (SHR). Following 8-11 weeks of DSR (n=15) or sedentary control (SED, n=15), rats were instrumented with arterial and venous catheters and mesenteric and iliac Doppler ultrasonic flow probes. Hemodynamic responses to vasodilator-mediated substances were determined under two experimental conditions; 1) bolus injection of indomethacin (10 mg/kg) and 4 bolus doses of acetylcholine (0.5-2.0 microg/kg); 2) bolus injection of N(omega)-nitro-L-arginine (5 mg/kg) and 4 bolus doses of nitroglycerin (3-12 microg/kg). Hindlimb vascular conductance decreased more in response to indomethacin in DSR vs. SED rats (-18.3+/-2.8% vs. -10.4+/-2.5%). However, the mesenteric or hindlimb vascular conductance responses to N(omega)-nitro-L-arginine were not different between DSR and SED rats. DSR also enhanced mesenteric and hindlimb vascular conductance responses to acetylcholine. Results suggest that DSR enhances acetylcholine-induced vasodilation in SHR.
Chen
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Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Collins
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DiCarlo
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D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D005500:Follow-Up Studies; D005730:Ganglionic Blockers / Q000494:pharmacology; D018738:Hexamethonium / Q000494:pharmacology; D006614:Hindlimb / Q000098:blood supply; D006973:Hypertension / Q000188:drug therapy / Q000503:physiopathology*; D007083:Iliac Artery / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008638:Mesenteric Arteries / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D019335:Nitroarginine / Q000494:pharmacology; D005996:Nitroglycerin / Q000494:pharmacology; D010805:Physical Conditioning, Animal; D051381:Rats; D011918:Rats, Inbred SHR; D018608:Ultrasonography, Doppler; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D005730:Ganglionic Blockers; D014665:Vasodilator Agents; D019335:Nitroarginine; D018738:Hexamethonium; D005996:Nitroglycerin; D000109:Acetylcholine; D007213:Indomethacin