Germ cell transfer into rat, bovine, monkey and human testes.
Germ cell transplantation is a potentially valuable technique offering oncological patients gonadal protection by reinitiating spermatogenesis from stem cells which were reinfused into the seminiferous tubules. In order to achieve an intratubular germ cell transfer, intratubular microinjection, efferent duct injections and rete testis injections were applied on dissected testes of four different species: rat, bull, monkey and man. Ultrasound-guided intratesticular rete testis injection was the best and least invasive injection technique with maximal infusion efficiency for larger testes. Deep infiltration of seminiferous tubules was only achieved in immature or partially regressed testes. This technique was applied in vivo on two cynomolgus monkeys. In the first monkey a deep infusion of injected cells and dye into the lumen of the seminiferous tubules was achieved. In the second, transplanted germ cells were present in the seminiferous epithelium 4 weeks after the transfer. These cells were morphologically identified as B-spermatogonia and located at the base of the seminiferous epithelium. In summary, this paper describes a promising approach for germ cell infusion into large testes. The application of this technique is the first successful attempt of a germ cell transfer in a primate.
Schlatt
S
S
Institute of Reproductive Medicine of the University, Münster, Germany.
Rosiepen
G
G
Weinbauer
G F
GF
Rolf
C
C
Brook
P F
PF
Nieschlag
E
E
D000818:Animals; D002417:Cattle; D006801:Humans; D066298:In Vitro Techniques; D007267:Injections; D008252:Macaca fascicularis; D008297:Male; D008845:Microinjections; D051381:Rats; D012671:Seminiferous Tubules / Q000601:surgery; D013094:Spermatozoa / Q000637:transplantation*; D013737:Testis / Q000601:surgery*