Role of lactosyl glycan sequences in inhibiting enteropathogenic Escherichia coli attachment.
Previously, we found that asialo-lactosamine sequences served as receptors for enteropathogenic Escherichia coli (EPEC) binding to Chinese hamster ovary (CHO) cells. In the present report, we have extended these earlier results by examining the ability of lactosamine- or fucosylated lactosamine-bovine serum albumin (BSA) glycoconjugates to inhibit EPEC, strain E2348/69, binding to HEp-2 cells. We found that, consistent with our previous findings with CHO cells, N-acetyllactosamine-BSA was the most effective inhibitor of EPEC localized adherence to HEp-2 cells, with Lewis X-BSA being the next best inhibitor. Further investigation revealed that coincubating EPEC E2348/69 with these BSA glycoconjugates alone caused a decrease in the expression of the bundle-forming pilus structural subunit (BfpA) and intimin by the bacteria. BfpA and intimin expression were reduced to the greatest extent by N-acetyllactosamine-BSA and Lewis X-BSA, respectively. These results suggest that the glycoconjugate inhibition of EPEC binding to HEp-2 cells might be achieved, wholly or in part, by an active mechanism that is distinct from simple competitive antagonism of receptor-adhesin interactions.
Vanmaele
R P
RP
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Heerze
L D
LD
Armstrong
G D
GD
D000606:Amino Sugars / Q000494:pharmacology; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology*; D001665:Binding Sites; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000378:metabolism*; D006001:Glycoconjugates / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D016256:Lewis X Antigen / Q000494:pharmacology; D009844:Oligosaccharides / Q000378:metabolism / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000606:Amino Sugars; D006001:Glycoconjugates; D016256:Lewis X Antigen; D009844:Oligosaccharides; C032233:lactosamine; D012710:Serum Albumin, Bovine