title: Ultrastructural correlates of quantal synaptic function at single CNS synapses.
abstract: We have tested the hypothesis that functional differences between synapses are associated with ultrastructure in cultured cortical neurons. Using Ca(2+) imaging, we measured NMDA receptor-mediated miniature synaptic calcium transients attributed to the spontaneous release of single transmitter quanta. After imaging, the identified neurons were processed for serial transmission electron microscopy. At sites of quantal NMDA receptor-dependent Ca(2+) transients, we confirmed the presence of excitatory synapses and measured spine size and synaptic contact area. Our results demonstrate that synapse size correlates positively with the amplitude of the NMDA receptor-mediated postsynaptic response, suggesting that larger synapses express a greater number of NMDA receptors. Therefore, regulation of quantal amplitude may involve processes that alter synapse size.
authors.affiliation: Kinsmen Laboratory of Neurological Research, Departments of Psychiatry and Physiology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Mackenzie
authors.forename: G S
authors.initials: GS
authors.lastname: Kenner
authors.forename: O
authors.initials: O
authors.lastname: Prange
authors.forename: H
authors.initials: H
authors.lastname: Shayan
authors.forename: M
authors.initials: M
authors.lastname: Umemiya
authors.forename: T H
authors.initials: TH
authors.lastname: Murphy
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology / Q000648:ultrastructure*; D019706:Excitatory Postsynaptic Potentials; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009457:Neuroglia / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D002118:Calcium