Effect of antidepressant drugs on veratridine-evoked glutamate and aspartate release in rat prefrontal cortex.
In vivo microdialysis in conscious rats was used to evaluate the effect of local application, through a microdialysis probe, of desipramine (DMI), imipramine and citalopram (CIT), on veratridine-evoked glutamate and aspartate release in rat prefrontal cortex (PFCx). All antidepressant drugs (ADs), given at a concentration of 0.1 mM, significantly inhibited glutamate release, while aspartate release was affected only by DMI and CIT. In contrast, local administration of ADs markedly potentiated veratridine-evoked dopamine and noradrenaline release. Perfusion of clonidine, quinpirole and 1-[3-(trifluoro-methyl)phenyl]-piperazine (TFMPP) at 0.1 mM concentration also diminished, evoked release of glutamate and aspartate. The regulation of amino acid release in rat PFCx may be achieved by direct effect of ADs on Na+ channels or indirectly, by involvement of D2/D3, alpha 2 or 5-HT1B heteroceptors activated by the increased level of monoamines in response to the blockade of respective transporters.
Gołembiowska
K
K
Department of Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland.
Zylewska
A
A
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001224:Aspartic Acid / Q000032:analysis / Q000378:metabolism*; D015283:Citalopram / Q000494:pharmacology; D003891:Desipramine / Q000494:pharmacology; D004298:Dopamine / Q000032:analysis; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism*; D007099:Imipramine / Q000494:pharmacology; D008297:Male; D017551:Microdialysis / Q000379:methods; D009638:Norepinephrine / Q000032:analysis; D010879:Piperazines / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D013997:Time Factors; D014701:Veratridine / Q000494:pharmacology
D000928:Antidepressive Agents; D010879:Piperazines; D017366:Serotonin Receptor Agonists; D015283:Citalopram; C018406:1-(3-trifluoromethylphenyl)piperazine; D001224:Aspartic Acid; D018698:Glutamic Acid; D014701:Veratridine; D007099:Imipramine; D003891:Desipramine; D004298:Dopamine; D009638:Norepinephrine