title: Detection of specific antibodies against human cultured chondrosarcoma (HCS-2/8) and osteosarcoma (Saos-2) cells in the serum of patients with osteoarthritis of the temporomandibular joint.
abstract: To find specific humoral antibodies in sera from patients with temporomandibular joint (TMJ) osteoarthritis (OA), an immortal human chondrocyte (HCS-2/8) and osteoblast (Saos-2) cell line derived from a chondrosarcoma and an osteosarcoma, respectively, were used as source proteins of human antigens. Patients with chronically painful TMJ OA (n = 18) but no other joints symptoms were selected from a consecutive series of patients with temperomandibular disorders and sex-matched asymptomatic controls (n = 8) were also recruited. Cellular proteins of the HCS-2/8 and Saos-2 cells were subjected to Western blotting with the OA and control sera as probes. Band-recognition frequency and the peak optical density of the band were compared between groups by chi2 and t-tests. OA sera recognized various bands for the chondrocytes, and one of these (47-kDa) was specific for the OA sera. In two OA patients whose treatment outcome was less favorable, the reactivity against the 47-kDa protein was relatively high. In addition, the OA sera clearly cross-reacted with recombinant HSP47. Based on these findings, an autoimmune reaction against chondrocytes could be one of the exaggerating and/or perpetuating mechanisms in the pathophysiology of osteoarthritic TMJs, and the humoral antibody titre against the HSP47-like protein derived from the chondrocytes could be one of the possible markers for the prognosis of the joint pathology.
authors.affiliation: Department of Fixed Prosthodontics, Japan. kuboki@dent.okayama-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kuboki
authors.forename: T
authors.initials: T
authors.lastname: Hattori
authors.forename: T
authors.initials: T
authors.lastname: Mizushima
authors.forename: M
authors.initials: M
authors.lastname: Kanyama
authors.forename: T
authors.initials: T
authors.lastname: Fujisawa
authors.forename: A
authors.initials: A
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood*; D001323:Autoantibodies / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D019902:Chondrocytes / Q000276:immunology; D002813:Chondrosarcoma / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood / Q000276:immunology*; D010006:Osteoblasts / Q000276:immunology; D012516:Osteosarcoma / Q000276:immunology*; D011379:Prognosis; D011994:Recombinant Proteins; D013705:Temporomandibular Joint Disorders / Q000097:blood / Q000276:immunology*; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D015415:Biomarkers; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; C494660:SERPINH1 protein, human