title: Isolation and quantification of tuliposides and tulipalins in tulips (Tulipa) by high-performance liquid chromatography.
abstract: The content of tuliposides and tulipalins were determined in Tulipa species and cultivars by reversed-phase high-performance liquid chromatography (RP-HPLC), using a water:methanol gradient as mobile phase. The compounds were detected by a diode array detector employed at 208 nm. The investigation revealed, in addition to 1- and 6-tuliposide A, tuliposide D and the lactonized aglycones tulipalin A and (-)-tulipalin B, the new tuliposide F and 6-tuliposide B, the latter being a new acyl derivative of the known 1-tuliposide B. All compounds were isolated by preparative RP-HPLC and identified by NMR and mass spectroscopy. The predominant compounds were 6-tuliposide A and B present in amounts up to 1.5% and 1.3% of fresh weight, respectively. 6-Tuliposide A and tulipalin A seem to be the major allergens in tulips, although tuliposide D and F may also contribute to the allergenic properties. Tulipalin A and (-)-tulipalin B occur in intact tulips and are not only produced in response to fungal attack or after excision of the plants. A few species were found to have very low allergen content and a relatively high level of tuliposide B, indicating it should be possible to breed non-allergenic and disease-resistant tulips.
authors.affiliation: Department of Fruit, Vegetable and Food Science, Danish Institute of Agricultural Sciences, Aarslev.
authors.forename: L P
authors.initials: LP
authors.lastname: Christensen
authors.forename: K
authors.initials: K
authors.lastname: Kristiansen
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry*; D012978:Sodium Oxybate / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000485:Allergens; D005960:Glucosides; D006027:Glycosides; C097922:tuliposide D; C048810:tulipalin B; C062516:tuliposide A; C014096:alpha-methylene gamma-butyrolactone; D012978:Sodium Oxybate; D015107:4-Butyrolactone