Genetic dissection of "OLETF," a rat model for non-insulin-dependent diabetes mellitus: quantitative trait locus analysis of (OLETF x BN) x OLETF.
To identify genetic determinants relevant to non-insulin-dependent diabetes mellitus (NIDDM), we performed a genome-wide analysis for quantitative trait loci (QTLs) using 359 backcross progeny of the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. The OLETF strain is a well-studied animal model of obese NIDDM, with features of hyperinsulinemia, hyperglycemia, insulin resistance, and abundant abdominal fat. Our extensive genomic scanning with 218 markers revealed nine significant QTLs, including a strong determinant of obesity on chromosome 1 (Dmo1: LOD = 13.99, for body weight). Two highly significant QTLs for glucose homeostasis were found, one on chromosome 1 (Dmo4 LOD = 7.16, for postprandial glucose level) and the other on chromosome X (Dmo11/Odb1: LOD = 7.81, for postprandial glucose level). These data are comparable to results of our previous studies of the OLETF rat.
Watanabe
T K
TK
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima, 771-0192, Japan.
Okuno
S
S
Oga
K
K
Mizoguchi-Miyakita
A
A
Tsuji
A
A
Yamasaki
Y
Y
Hishigaki
H
H
Kanemoto
N
N
Takagi
T
T
Takahashi
E
E
Irie
Y
Y
Nakamura
Y
Y
Tanigami
A
A
D000273:Adipose Tissue; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D008126:Lod Score; D008297:Male; D009765:Obesity / Q000235:genetics; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D051381:Rats; D011914:Rats, Inbred BN; D020334:Rats, Inbred OLETF; D014960:X Chromosome / Q000235:genetics
D001786:Blood Glucose