title: [Angiotensin II receptor subtype in human adrenal glands].
abstract: Although adrenal gland is one of the major target organs of angiotensin II (Ang II), the pathophysiological significance of the its receptor subtype has not been elucidated. We demonstrated by reverse transcription-polymerase chain reaction with Southern blot analysis mRNA expression of both AT1 and AT2 in human adrenal tissues of normal adrenocortical tissues, aldosterone-producing adenoma, Cushing's syndrome, and pheochromocytoma. Ang II-induced aldosterone secretion in vitro was suppressed only by 50% in the presence of selective AT1 antagonist CV-11974, while AT2 agonist CGP-42112 increased aldosterone secretion by 55% over the control. Ang II or CGP-42112 did not affect cortisol secretion. In addition, Ang II could stimulate aldosterone secretion in AT1a knockout mice both in the presence and absence of CV-11974. These results suggest that non-AT1 receptor subtype(s) including AT2, as well as AT1, is involved in the stimulation of aldosterone secretion from human adrenals.
authors.affiliation: Department of Medicine, Tokyo Women's Medical University.
authors.forename: A
authors.initials: A
authors.lastname: Tanabe
authors.forename: M
authors.initials: M
authors.lastname: Naruse
authors.forename: K
authors.initials: K
authors.lastname: Naruse
authors.forename: T
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Mishina
authors.forename: T
authors.initials: T
authors.lastname: Imaki
authors.forename: K
authors.initials: K
authors.lastname: Sugaya
authors.forename: H
authors.initials: H
authors.lastname: Miyazaki
authors.forename: H
authors.initials: H
authors.lastname: Demura
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000311:Adrenal Glands / Q000737:chemistry*; D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D011945:Receptors, Angiotensin / Q000032:analysis* / Q000145:classification / Q000502:physiology
chemical_list: D011945:Receptors, Angiotensin; D000450:Aldosterone