[Selective suppression of polymerase chain reaction].
The selective suppression of the polymerase chain reaction and methods based upon it (construction of cDNA libraries from low amounts of biological material, subtractive hybridization and differential display of mRNA, fast cloning of full-size cDNA, chromosome walking, cloning in vitro, and others) are reviewed. These methods display a high effectiveness and, taken together, enable intricate DNA analyses to be performed--from the search for nontrivial sequences to the total sequencing of the corresponding genes.
Luk'ianov
K A
KA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. luk@ibch.siobc.ras.ru
Gurskaia
N G
NG
Bogdanova
E A
EA
Luk'ianov
S A
SA
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D016386:Chromosome Walking; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D005786:Gene Expression Regulation; D015723:Gene Library; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D013489:Suppression, Genetic
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary
Selektivnaia supressiia polimeraznoĭ tsepnoĭ reaktsii.