Rapid fabrication of plastic-insulated carbon-fiber electrodes for micro-amperometry.
Carbon-fiber amperometry and voltammetry are useful techniques to measure secretion of oxidizable neurotransmitters from neurosecretory cells. Recent applications with probes of small geometry permit detection of the exocytosis of single secretory vesicles in individual cells. We have developed a semi-automatic puller and cutter to prepare such plastic-insulated electrodes efficiently with various sizes of carbon fibers. The electrodes are smooth, reproducible, and easy to make.
Koh
D S
DS
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195-7290, USA. koh@u.washington.edu
Hille
B
B
D000588:Amines; D000818:Animals; D002244:Carbon; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004847:Epithelial Cells / Q000378:metabolism; D005089:Exocytosis; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D009490:Neurosecretory Systems / Q000166:cytology / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D010969:Plastics; D013572:Synaptic Vesicles / Q000378:metabolism
D000588:Amines; D018377:Neurotransmitter Agents; D010969:Plastics; D002244:Carbon