title: Protective effects of Ginkgo biloba extract against lysophosphatidylcholine-induced vascular endothelial cell damage.
abstract: OBJECTIVE
  To study the protective effects of Ginkgo biloba extract (GbE) against endothelial cell damage induced by lysophosphatidylcholine (LPC).
  
  
  METHODS
  The vasorelaxation response to acetylcholine (ACh) were investigated in the isolated rabbit thoracic aorta. Lipid peroxidation products were determined by measuring thiobarbituric acid reactive substance.
  
  
  RESULTS
  GbE attenuated the inhibition of vasorelaxation response to ACh and prevented the LPC-induced increase of malondialdehyde (MDA) content both in thoracic aortae. GbE prevented the leakage of LDH and the increase of MDA content in cultured endothelial cells in a concentration-dependent manner. GbE also markedly increased epoprostenol level in cultured endothelial cells treated with LPC.
  
  
  CONCLUSIONS
  GbE protected endothelial cells against LPC-induced damage due to reduction in lipid peroxidation and facilitation of synthesis and/or release of epoprostenol.
authors.affiliation: Department of Pharmacology, Hengyang Medical College, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Chen
authors.forename: W Z
authors.initials: WZ
authors.lastname: Chen
authors.forename: H L
authors.initials: HL
authors.lastname: Huang
authors.forename: L X
authors.initials: LX
authors.lastname: Chen
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Xie
authors.forename: B Y
authors.initials: BY
authors.lastname: Zhu
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010946:Plants, Medicinal; D011817:Rabbits; D014664:Vasodilation / Q000187:drug effects
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D046934:Ginkgolides; D007783:Lactones; D008244:Lysophosphatidylcholines; D008315:Malondialdehyde; D015121:6-Ketoprostaglandin F1 alpha; C045856:ginkgolide B; D007770:L-Lactate Dehydrogenase