title: Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme.
abstract: BACKGROUND
  Monomeric sarcosine oxidases (MSOXs) are among the simplest members of a recently recognized family of eukaryotic and prokaryotic enzymes that catalyze similar oxidative reactions with various secondary or tertiary amino acids and contain covalently bound flavins. Other members of this family include heterotetrameric sarcosine oxidase, N-methyltryptophan oxidase and pipecolate oxidase. Mammalian sarcosine dehydrogenase and dimethylglycine dehydrogenase may be more distantly related family members.
  
  
  RESULTS
  The X-ray crystal structure of MSOX from Bacillus sp. B-0618, expressed in Escherichia coli, has been solved at 2.0 A resolution by multiwavelength anomalous dispersion (MAD) from crystals of the selenomethionine-substituted enzyme. Fourteen selenium sites, belonging to two MSOX molecules in the asymmetric unit, were used for MAD phasing and to define the local twofold symmetry axis for electron-density averaging. The structures of the native enzyme and of two enzyme-inhibitor complexes were also determined.
  
  
  CONCLUSIONS
  MSOX is a two-domain protein with an overall topology most similar to that of D-amino acid oxidase, with which it shares 14% sequence identity. The flavin ring is located in a very basic environment, making contact with sidechains of arginine, lysine, histidine and the N-terminal end of a helix dipole. The flavin is covalently attached through an 8alpha-S-cysteinyl linkage to Cys315 of the catalytic domain. Covalent attachment is probably self-catalyzed through interactions with the positive sidechains and the helix dipole. Substrate binding is probably stabilized by hydrogen bonds between the substrate carboxylate and two basic sidechains, Arg52 and Lys348, located above the re face of the flavin ring.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Ave, St. Louis, MO 63110, USA.
authors.forename: P
authors.initials: P
authors.lastname: Trickey
authors.forename: M A
authors.initials: MA
authors.lastname: Wagner
authors.forename: M S
authors.initials: MS
authors.lastname: Jorns
authors.forename: F S
authors.initials: FS
authors.lastname: Mathews
mesh_terms: D000085:Acetates / Q000737:chemistry / Q000494:pharmacology; D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D002384:Catalysis; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry*; D011392:Proline / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D050879:Sarcosine Oxidase; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D011993:Recombinant Fusion Proteins; D005182:Flavin-Adenine Dinucleotide; C118653:(methylthio)acetic acid; C002986:2-pyrrolecarboxylic acid; D011392:Proline; D010089:Oxidoreductases, N-Demethylating; D050879:Sarcosine Oxidase