title: Characterization of eae+ Escherichia coli isolated from healthy and diarrheic calves.
abstract: Strains of Escherichia coli from 101 healthy and 114 diarrheic calves were screened by PCR for the eae (intimin) gene and Shiga toxin genes (stx). Each eae+ and eae/stx+ strain was examined for antimicrobial susceptibility, enterohemolysin activity, and the somatic O antigen was determined. An immunoassay was used to detect Shiga toxin antigens for the eae/stx+ E. coli. Significantly more (p = 0.005) of the healthy calves carried eae+ and eae/stx+ E. coli in their feces when compared to strains from diarrheic calves. Moreover, Shiga toxin antigens were detected significantly more (p = 0.001) often among the eae/stx+ strains from healthy calves when compared to eae/stx+ strains from diarrheic calves. However, significantly more (p = 0.001) of the eae+ and eae/stx+ strains from diarrheic calves were resistant to at least one of the antimicrobials tested, and the strains from diarrheic calves had a significantly (p = 0.05) higher rate of antimicrobial resistance to at least two different antimicrobial classes. No significant difference (p> or =0.05) was detected among the eae+ and eae/stx+ strains from healthy and diarrheic calves for enterohemolysin production. Serogroups O-negative, O5, O26, and O111 were predominate among both healthy and diarrheic calves.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, 48824, USA. rholland@cvm.msu.edu
authors.forename: R E
authors.initials: RE
authors.lastname: Holland
authors.forename: R A
authors.initials: RA
authors.lastname: Wilson
authors.forename: M S
authors.initials: MS
authors.lastname: Holland
authors.forename: V
authors.initials: V
authors.lastname: Yuzbasiyan-Gurkan
authors.forename: T P
authors.initials: TP
authors.lastname: Mullaney
authors.forename: D G
authors.initials: DG
authors.lastname: White
mesh_terms: D018829:Adhesins, Bacterial; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics; D002352:Carrier Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D005260:Female; D006460:Hemolysin Proteins / Q000032:analysis; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007422:Intestines / Q000473:pathology; D008824:Michigan; D016133:Polymerase Chain Reaction / Q000662:veterinary; D022603:Shiga Toxins
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D022603:Shiga Toxins; C073819:eaeA protein, E coli