Protective effects of Ginkgo biloba extract against lysophosphatidylcholine-induced vascular endothelial cell damage.
OBJECTIVE
To study the protective effects of Ginkgo biloba extract (GbE) against endothelial cell damage induced by lysophosphatidylcholine (LPC).


METHODS
The vasorelaxation response to acetylcholine (ACh) were investigated in the isolated rabbit thoracic aorta. Lipid peroxidation products were determined by measuring thiobarbituric acid reactive substance.


RESULTS
GbE attenuated the inhibition of vasorelaxation response to ACh and prevented the LPC-induced increase of malondialdehyde (MDA) content both in thoracic aortae. GbE prevented the leakage of LDH and the increase of MDA content in cultured endothelial cells in a concentration-dependent manner. GbE also markedly increased epoprostenol level in cultured endothelial cells treated with LPC.


CONCLUSIONS
GbE protected endothelial cells against LPC-induced damage due to reduction in lipid peroxidation and facilitation of synthesis and/or release of epoprostenol.
Chen
J X
JX
Department of Pharmacology, Hengyang Medical College, China.
Chen
W Z
WZ
Huang
H L
HL
Chen
L X
LX
Xie
Z Z
ZZ
Zhu
B Y
BY
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010946:Plants, Medicinal; D011817:Rabbits; D014664:Vasodilation / Q000187:drug effects
D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D046934:Ginkgolides; D007783:Lactones; D008244:Lysophosphatidylcholines; D008315:Malondialdehyde; D015121:6-Ketoprostaglandin F1 alpha; C045856:ginkgolide B; D007770:L-Lactate Dehydrogenase