Organization of telomeric and sub-telomeric regions of chromosomes from the protozoan parasite Trypanosoma cruzi.
We present here a characterization of the telomeric and subtelomeric regions of Trypanosoma cruzi chromosomes, using three types of recombinants: cosmids from a genomic library, clones obtained by a vector-adaptor protocol, and a recombinant fragment cloned by a Bal31 trimming protocol. The last nine nucleotides of the T. cruzi overhang are 5'-GGGTTAGGG-3', and there are from 9 to 50 copies of the hexameric repeat 5'-TTAGGG-3', followed by a 189-bp junction sequence common to all recombinants. The subtelomeric region is made of sequences associated with the gp85/sialidase gene family, and/or sequences derived from SIRE, a retrotransposon-like sequence, and also the retrotransposon L1Tc. We discuss the possible implications of this genome organization.
Chiurillo
M A
MA
Instituto de Biologia Experimental, Universidad Central de Venezuela, Caracas.
Cano
I
I
Da Silveira
J F
JF
Ramirez
J L
JL
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics; D011994:Recombinant Proteins; D015183:Restriction Mapping; D018626:Retroelements / Q000235:genetics; D016615:Telomere / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics
D016054:DNA, Protozoan; D011994:Recombinant Proteins; D018626:Retroelements; D014643:Variant Surface Glycoproteins, Trypanosoma; D009439:Neuraminidase