Stimulation of prolactin release by prolactin-releasing peptide in rats.
We have previously reported a hypothalamic peptide that shows specific prolactin (PRL)-releasing activity in vitro, named prolactin-releasing peptide (PrRP). However, its activity in vivo has not yet been shown. In this study, we examined whether PrRP could induce specific PRL release in vivo using normal cycling female and male rats. Intravenous injection of PrRP31 increased plasma PRL levels in rats in a dose-dependent manner. PrRP31 (50 nmol/kg i.v.) significantly (P < 0.05) stimulated plasma PRL levels within 25 min after injection in rats in proestrus, estrus, and metestrus. A higher dose of PrRP31 (500 nmol/kg i.v.) was necessary for a significant increase in plasma PRL levels in male rats. These results clearly indicate that female rats, especially at proestrus, are more sensitive to PrRP-induced PRL secretion than male rats. The effect of PrRP on PRL release is affected considerably by the estrous cycle and sex, which suggests that PrRP sensitivity is controlled by the endogenous hormonal milieu, such as estrogen levels. PrRP31 did not affect other pituitary hormone secretions. The results indicate that PrRP shows specific PRL-releasing activity in vivo as well as in vitro and suggest that it plays an important role in the regulation of PRL release under certain physiological conditions.
Matsumoto
H
H
Pharmaceutical Discovery Division, Takeda Chemical Industries Ltd., Wadai-10, Tsukuba, Ibaraki, 300-4293, Japan. Matsumoto_Hirokazu@takeda.co.jp
Noguchi
J
J
Horikoshi
Y
Y
Kawamata
Y
Y
Kitada
C
C
Hinuma
S
S
Onda
H
H
Nishimura
O
O
Fujino
M
M
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D004971:Estrus / Q000097:blood; D005260:Female; D007028:Hypothalamic Hormones / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D011388:Prolactin / Q000097:blood*; D056690:Prolactin-Releasing Hormone; D011863:Radioimmunoassay; D051381:Rats; D011916:Rats, Inbred F344; D013972:Thyrotropin / Q000097:blood
D007028:Hypothalamic Hormones; D009479:Neuropeptides; C534379:Prlh protein, rat; D056690:Prolactin-Releasing Hormone; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D013972:Thyrotropin