Targeting genes for self-excision in the germ line.
A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker Neor, and the two genes flanked with loxP elements. This cassette was targeted to the Hoxa3 gene in mouse ES cells that were in turn used to generate chimeric mice. In these chimeras, somatic cells derived from the ES cells retained the cassette, but self-excision occurred in all ES-cell-derived sperm.
Bunting
M
M
Hematology Division, Department of Internal Medicine, University of Utah, Salt Lake City, Utah 84112, USA.
Bernstein
K E
KE
Greer
J M
JM
Capecchi
M R
MR
Thomas
K R
KR
D000818:Animals; D001483:Base Sequence; D004249:DNA Damage; D018076:DNA, Complementary; D005260:Female; D018390:Gene Targeting / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics*; D019426:Integrases / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013094:Spermatozoa; D013737:Testis; D014764:Viral Proteins
D018076:DNA, Complementary; D018398:Homeodomain Proteins; C080331:Hoxa3 protein, mouse; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases; D007703:Peptidyl-Dipeptidase A