Staurosporine enhances cAMP-induced expression of neural-specific gene VGF and tyrosine hydroxylase.
Cyclic AMP transiently stimulates transcription of specific genes in the nervous system, including neural-specific gene VGF. The simultaneous presence of staurosporine (SSP) caused a progressive, rather than transient, increase in VGF mRNA levels during the cAMP-induced differentiation of human neuroblastoma cells. This steady increase does not appear to be due to increased stability of VGF mRNA. This synergy between cAMP and SSP was also observed at the protein level for tyrosine hydroxylase. These findings suggest the presence of cellular machinery that counteracts the decline of cAMP-induced gene expression.
Nagasaki
K
K
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan. knagasak@gan2.ncc.go.jp
Sasaki
K
K
Maass
N
N
Tsukada
T
T
Hanzawa
H
H
Yamaguchi
K
K
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides; D011506:Proteins / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019311:Staurosporine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D004791:Enzyme Inhibitors; D009479:Neuropeptides; D011506:Proteins; D012333:RNA, Messenger; C062040:Vgf protein, rat; D000242:Cyclic AMP; D014446:Tyrosine 3-Monooxygenase; D019311:Staurosporine