[Expression of a new family of receptors similar to CXC chemokine receptors in endothelial cell precursors].
Characterization of a new family of G protein-coupled receptors is reported. Expression of these receptors is associated with endothelial lineage. Cloning of the Xenope X-msr receptor allowed to show that embryonic expression of this receptor occurred in the heart and developing primary blood vessels. Furthermore, within these cardiovascular structures, expression was restricted to the endothelial layer. Because structural similarities with the human orphan receptor h-APJ were found, the msr/apj receptor was cloned in mice. This showed that embryonic expression of this receptor was also confirmed to endothelial precursors. Thus, this receptor is the orthological equivalent in mice to the amphibian receptor X-msr. Molecular phylogenesis studies showed that the X-msr, msr/apj, and h-APJ receptors shared considerable homology with two CXC chemokine receptors, namely LCR1, whose name was recently changed to CXCR4, and RDC1, which is structurally similar to the CXCR2 receptor. The human h-APJ receptor is a co-receptor for entry of the HIV into T cells, a property associated only with CXC chemokine receptors in the lymphocyte population. These data suggest that this new signaling system may participate in endothelial precursor migration during developmental angiogenesis and in endothelial cell migration and proliferation during neoangiogenesis in adults.
Devic
E
E
Unité INSERM U-397, CHU Rangueil, Toulouse, France.
Rizzoti
K
K
Bodin
S
S
Paquereau
L
L
Knibiehler
B
B
Audigier
Y
Y
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000075244:Apelin Receptors; D004730:Endothelium, Vascular / Q000196:embryology / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D043562:Receptors, G-Protein-Coupled; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000378:metabolism*; D014981:Xenopus; D029867:Xenopus Proteins
C105530:APLNR protein, Xenopus; C104575:APLNR protein, human; D000075244:Apelin Receptors; C491285:Aplnr protein, mouse; D011956:Receptors, Cell Surface; D019707:Receptors, Chemokine; D043562:Receptors, G-Protein-Coupled; D029867:Xenopus Proteins; D019204:GTP-Binding Proteins
Expression d'une nouvelle famille de récepteurs apparentés aux récepteurs des CXC chémokines dans des précurseurs endothéliaux.