Effect of histidine on myocardial mitochondria and platelet aggregation during thrombotic cerebral ischemia in rats.
OBJECTIVE
To study the effect of histidine on cerebral thrombosis and possible mechanism.


METHODS
Cerebral-cardiac stroke was produced by photochemically induced thrombotic cerebral ischemia in rats.


RESULTS
Platelet aggregation in whole blood increased markedly, peak heights at 4 and 24 h were (5.1 +/- 0.5) omega and (4.3 +/- 0.5) omega, respectively. Heart mitochondria volume (V), volume density (Vv), surface density (Nm), and surface density of outer membrane (Sv1) increased (8.2 +/- 5.5, 0.59 +/- 0.16, 0.11 +/- 0.03, and 0.22 +/- 0.05, respectively, P < 0.01), but numerical density (Nv), specific surface of inner membrane (delta 2) and of the cristae (delta 3) decreased (0.07 +/- 0.02, 2.8 +/- 0.8, and 2.4 +/- 0.7, respectively, P < 0.01) after cerebral thrombosis. The myocardial histopathologic characteristics were different from those of ischemic necrosis and myocardial damage caused by ischemic reperfusion. In rat treated with histidine after photochemical reaction, platelet aggregation decreased markedly [(2.93 +/- 1.08) omega, P < 0.01], reversible change often went with parameters related to the inner mitochondrial membrane but not the outer mitochondrial membrane.


CONCLUSIONS
Histidine depressed platelet aggregation and reduced myocardial mitochondrial damage resulted from cerebral ischemia.
Li
S Q
SQ
Department of Pathophysiology, Kunming Medical College, China.
Zhao
G
G
Li
J
J
Qian
W
W
D000818:Animals; D006639:Histidine / Q000494:pharmacology*; D002542:Intracranial Embolism and Thrombosis / Q000097:blood / Q000473:pathology*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects*; D008933:Mitochondrial Swelling; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010975:Platelet Aggregation Inhibitors; D006639:Histidine