title: DGGE/TGGE a method for identifying genes from natural ecosystems.
abstract: Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population dynamics. Recent advances in these techniques have demonstrated their importance in microbial ecology.
authors.affiliation: Netherlands Institute for Sea Research NL-1790 AB Den Burg, The Netherlands. gmuyzer@nioz.nl
authors.forename: G
authors.initials: G
authors.lastname: Muyzer
mesh_terms: D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004783:Environmental Microbiology; D005796:Genes / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S