title: Effect of mitoxantrone on DNA polymerase of Ehrlich ascites carcinoma cells.
abstract: OBJECTIVE
  To study the effect of mitoxantrone (Mit) on DNA polymerases of tumor cells.
  
  
  METHODS
  DNA polymerases of Ehrlich ascites carcinoma cells were isolated by phosphocellulose column chromatography. The effects of Mit on DNA polymerase alpha, beta, and gamma were detected by method of K Ono.
  
  
  RESULTS
  Mit inhibited DNA polymerase alpha, beta, and gamma, IC50 values were 11.9, 6.5, and 11.9 mumol.L-1, and Ki 1.86, 2.22, and 2.05 mumol.L-1, respectively. The inhibitory mode of Mit on DNA polymerase alpha, beta, and gamma was competitive.
  
  
  CONCLUSIONS
  Mit is a strong inhibitor on DNA polymerase alpha, beta, and gamma. The inhibitory mode was competition with respect to template DNA.
authors.affiliation: Cancer Institute, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Xian
authors.forename: H X
authors.initials: HX
authors.lastname: Li
authors.forename: Z C
authors.initials: ZC
authors.lastname: Liu
authors.forename: Q C
authors.initials: QC
authors.lastname: Pan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001667:Binding, Competitive; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology* / Q000473:pathology; D004256:DNA Polymerase I / Q000378:metabolism; D019951:DNA Polymerase beta / Q000378:metabolism; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D008942:Mitoxantrone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000201:enzymology
chemical_list: D000970:Antineoplastic Agents; D004317:Doxorubicin; D008942:Mitoxantrone; D004256:DNA Polymerase I; D019951:DNA Polymerase beta; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase