Three-colour, one-tube flow cytometric method for enumeration of CD4+ and CD8+ lymphocytes.
We investigated the usefulness of a one-tube, three-colour flow cytometric method for enumerating CD4+ and CD8+ lymphocytes. This method does not use any control antibodies and we compared it to the standard methods using either control and CD14/CD45 antibodies or control antibodies only on 38 blood samples from healthy and human immunodeficiency virus-infected patients. The one-tube method showed good agreement with the other, more complicated methods and is therefore suitable for reliable enumeration of CD4+ and CD8+ T-lymphocytes.
Janssen
W C
WC
Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands.
Hoffmann
J J
JJ
D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000032:analysis
D018950:Lipopolysaccharide Receptors; D017493:Leukocyte Common Antigens