A new monoclonal anti-idiotypic catalytic antibody with a CPA-like activity.
IIF9D8, a new monoclonal anti-idiotypic catalytic antibody with a CPA esterase-like activity was elicited by ID11D7, the monoclonal competitive inhibitory antibody to CPA. The hydrolysis of hippuryl-DL-phenyllactic acid by McAb IIF9D8 follows the Michaelis-Menten kinetics. The Km value and kcat are 0.036 M and 0.598 min(-1), respectively, and the rate acceleration (kcat/kuncat) is 30500. Compared with the previous McAb 32C3 induced by polyclonal antibodies to CPA, McAb IIF9D8 shows higher catalytic efficiency The catalytic antibodies with the catalytic properties similar to natural enzymes could be obtained by this approach.
Du
M
M
College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Guo
Z
Z
Jin
S
S
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D017151:Antibodies, Catalytic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002268:Carboxypeptidases / Q000378:metabolism*; D043422:Carboxypeptidases A; D004950:Esterases / Q000037:antagonists & inhibitors; D006626:Hippurates / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010666:Phenylpropionates / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D006626:Hippurates; D007773:Lactates; D010666:Phenylpropionates; D011480:Protease Inhibitors; C121218:hippurylphenyllactic acid; C035253:3-phenylpropionic acid; D004950:Esterases; D002268:Carboxypeptidases; D043422:Carboxypeptidases A