The fungicide carbendazim induces meiotic micronuclei in the spermatids of the rat testis.
Whether the fungicide carbendazim affects the meiotic spermatocytes and consequently induces chromosome aberrations in the spermatids was determined in the adult rat testis using the micronucleus test. Round spermatids containing micronuclei (MN) were significantly increased in number at stages I and V on days 1 and 4.5 after treatment with carbendazim (100 mg/kg), respectively (p<0.05). Immunocytochemistry indicated that approximately 68% of the carbendazim-induced MN contained kinetochores. These results suggest that carbendazim induces chromosome aberrations in spermatids with a high incidence of aneuploidy.
Matsuo
F
F
Department of Veterinary Anatomy, Faculty of Agriculture, Miyazaki University, Japan.
Nakai
M
M
Nasu
T
T
D000818:Animals; D001562:Benzimidazoles / Q000633:toxicity*; D002219:Carbamates; D005659:Fungicides, Industrial / Q000633:toxicity*; D008297:Male; D008540:Meiosis; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology*; D013087:Spermatids / Q000166:cytology / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000473:pathology*
D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; C006698:carbendazim