Cytokine gene expression following neural grafts.
A reverse-transcription polymerase chain reaction study of molecular events following solid neural tissue xenogeneic and syngeneic grafts into the mouse caudate nucleus was made between 1 day and 30 days post-grafting. Constitutive expression of interleukin (IL)-1 and transforming growth factor (TGF)-beta1 mRNAs was detected. The sham operation produced minimal cytokine upregulation, indicating that surgical trauma caused minimal damage. A similar picture was seen with syngeneic grafts, which showed good graft survival. Xenografts, however, were rejected within 30 days and cytokine mRNAs for IL-2, IL-2R, IL-4, IL-10 and interferon (IFN)-gamma were detected from 7 days post-graft, correlating with histological identification of a leukocytic infiltrate. This method provides a quick, comparative screen for detecting cytokine mRNA profiles in neural grafts and may assist the diagnosis of early rejection phenomena. As such, it is a potential analytical tool for strategies aimed at depleting/blocking the activity of different cell types and/or cytokines in future neural transplant therapy.
Vine
A M
AM
Department of Human Anatomy, University of Oxford, England. a.vine@ic.ac.uk
Lang
J
J
Wood
M J
MJ
Charlton
H M
HM
D000818:Animals; D002421:Caudate Nucleus / Q000378:metabolism / Q000502:physiology*; D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009417:Nerve Tissue / Q000502:physiology* / Q000637:transplantation*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous / Q000502:physiology; D014185:Transplantation, Isogeneic
D016207:Cytokines; D012333:RNA, Messenger