[mRNA expression of granulocyte-macrophage colony-stimulating factor in airway tissues of asthma guinea pigs: effect of triptolide].
OBJECTIVE
To explore the role of granulocyte-macrophage colony-stimulating factor (GM-CSF) in eosinophil inflammation of asthma airway.


METHODS
Guinea pig models of asthma were established with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with injection of triptolide, density of eosinophil infiltration in airway tissues was observed under microscope and expression of GM-CSF mRNA in airway tissues was detected with dot hybridization by Dig-labeled cDNA probe.


RESULTS
Expression of GM-CSF mRNA in asthmatic animals were higher markedly than that in the triptolide-treated and the control groups. However, there was no statistically differences of density of eosinophils infiltration between the asthma and the triptolide-treated groups.


CONCLUSIONS
GM-CSF might participate in eosinophil inflammation in airway of asthmatic animals and triptolide might be of potential value in anti-inflammation for asthma.
Jin
Y
Y
Department of Respiratory Disease, Lanzhou General Hospital of PLA.
Wang
C
C
Wang
C
C
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D006168:Guinea Pigs; D010047:Ovalbumin; D010616:Phenanthrenes; D012333:RNA, Messenger / Q000235:genetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D012333:RNA, Messenger; C001899:triptolide; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010047:Ovalbumin