Experimental study of hypovolaemic shock-induced gastric mucosal lesions in the rat.
This study was designed to determine whether oxygen-derived free radicals play a role in the pathogenesis of gastric mucosal lesions produced by haemorrhagic shock and reperfusion experimental model in the rat. Ranitidine (H2-receptor blocker) in different doses, allopurinol, an inhibitor of xanthine oxidase and SOD (superoxide dysmutase) pre-treatment were used against haemorrhagic shock and reperfusion induced gastric mucosal lesions. Altogether 67 rats were divided into seven different groups. The area of gastric mucosal lesions was measured, the activity of endogenous peroxidase was examined histochemically and histological grading was made. Evans blue was used to demonstrate the improved permeability of gastric mucosal membranes. Ranitidine, in high dose, allopurinol and superoxide dysmutase significantly protected against haemorrhagic shock-induced gastric mucosal lesions, against improved membrane permeability and peroxidation.
Zöllei
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Department of Surgery, Albert Szent-Györgyi Medical University, Szeged, Hungary. ZI@surg.szote.u-szeged.hu
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005753:Gastric Mucosa / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006651:Histocytochemistry; D008297:Male; D009195:Peroxidase / Q000032:analysis; D011899:Ranitidine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology; D012769:Shock / Q000473:pathology* / Q000503:physiopathology; D012771:Shock, Hemorrhagic / Q000473:pathology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D016166:Free Radical Scavengers; D005609:Free Radicals; D006635:Histamine H2 Antagonists; D000493:Allopurinol; D011899:Ranitidine; D009195:Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase