Retroviral vectors pseudotyped with lymphocytic choriomeningitis virus.
Pseudotyping can improve retroviral vector stability and transduction efficiency. Here, we describe a novel pseudotype of murine leukemia virus packaged with lymphocytic choriomeningitis virus (LCMV). This pseudotype was stable during ultracentrifugation and infected several cell lines from different species. Moreover, LCMV glycoproteins were not cell toxic.
Miletic
H
H
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, 20251 Hamburg, Germany.
Bruns
M
M
Tsiakas
K
K
Vogt
B
B
Rezai
R
R
Baum
C
C
Kühlke
K
K
Cosset
F L
FL
Ostertag
W
W
Lother
H
H
von Laer
D
D
D000818:Animals; D000956:Antigens, Viral; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D005822:Genetic Vectors / Q000502:physiology*; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000502:physiology*; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics; D014407:Tumor Cells, Cultured; D014764:Viral Proteins
D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins; C115679:glycoprotein peptide, Lymphocytic choriomeningitis virus