Evaluation of an Epstein-Barr virus (EBV) immunoglobulin M enzyme-linked immunosorbent assay using a synthetic convergent peptide library, or mixotope, for diagnosis of primary EBV infection.
An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.
Tranchand-Bunel
D
D
Laboratoire d'Immunologie Cellulaire, CNRS UMR 8527, Institut Pasteur de Lille, 59019 Lille, France. d.bunel@infobiogen.fr
Gras-Masse
H
H
Bourez
B
B
Dedecker
L
L
Auriault
C
C
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006566:Herpesviridae Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008969:Molecular Sequence Data; D019151:Peptide Library; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D019151:Peptide Library