Antichymotrypsin interaction with chymotrypsin. Intermediates on the way to inhibited complex formation.
Serpins form enzymatically inactive covalent complexes (designated E*I*) with their target proteinases, corresponding most likely to the acyl enzyme that resembles the normal intermediate in substrate turnover. Formation of E*I* involves large changes in the conformation of the reactive center loop (residues P17 to P9') and of the serpin molecule in general. The "hinge" region of the reactive center loop, including residues P10-P14, shows facile movement in and out of beta-sheet A, and this movement appears to be crucial in determining whether E*I* is formed (the inhibitor pathway) or whether I is rapidly hydrolyzed to I* (the substrate pathway). Here, we report stopped-flow and rapid quench studies investigating the pH dependence of the conversion of the alpha1-antichymotrypsin.alpha-chymotrypsin encounter complex, E.I, to E*I*. These studies utilize fluorescent derivatives of cysteine variants of alpha1-antichymotrypsin at the P11 and P13 residues. Our results demonstrate three identifiable intermediates, EIa, EIb, and EIc, between E.I and E*I* and permit informed speculation regarding the nature of these intermediates. Partitioning between inhibitor and substrate pathways occurs late in the process of E*I* formation, most likely from a species occurring between EIc and E*I*.
Luo
Y
Y
Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 191046323, USA.
Zhou
Y
Y
Cooperman
B S
BS
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010069:Oxadiazoles / Q000737:chemistry; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D000514:alpha 1-Antichymotrypsin / Q000737:chemistry*
D010069:Oxadiazoles; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D000514:alpha 1-Antichymotrypsin; C046364:4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole; D002918:Chymotrypsin