title: Cytogenetic and molecular characterization of T-cell acute lymphoblastic leukemia as a second tumor after anaplastic large-cell lymphoma in a boy.
abstract: We report a case of acute T-cell lymphoblastic leukemia which developed in a boy 8.5 years after successful treatment for anaplastic large-cell lymphoma. Cytogenetic and molecular characterizations of the second tumor were performed. The cytogenetic investigation revealed a complex pattern of karyotypic alterations, including double minutes, ring chromosomes, and a duplication of the p21-32 region of chromosome 1. The microsatellite DNA analysis excluded rearrangement or deletion of the TAL1 gene in the tumor cells; rearrangements of the MLL gene were excluded by Southern blot analysis. To the best of our knowledge, this is the first report of T-cell lymphoblastic leukemia arising after treatment of CD 30+ anaplastic large-cell lymphoma. The different T-cell receptor rearrangement evidenced in the two tumors indicates that this second malignancy most likely emerged de novo, but was plausibly related to the previous radiation and chemotherapy.
authors.affiliation: Division of Pediatric Oncology, Istituto Nazionale Tumori, via G. Venezian 1, 20133 Milan, Italy. perotti@istitutotumori.mi.it
authors.forename: D
authors.initials: D
authors.lastname: Perotti
authors.forename: G
authors.initials: G
authors.lastname: Sozzi
authors.forename: A
authors.initials: A
authors.lastname: Ferrari
authors.forename: M
authors.initials: M
authors.lastname: Casanova
authors.forename: F
authors.initials: F
authors.lastname: Gambirasio
authors.forename: P
authors.initials: P
authors.lastname: Mondini
authors.forename: A
authors.initials: A
authors.lastname: Mezzelani
authors.forename: R
authors.initials: R
authors.lastname: Giardini
authors.forename: F
authors.initials: F
authors.lastname: Pettenella
authors.forename: D
authors.initials: D
authors.lastname: Papini
authors.forename: A
authors.initials: A
authors.lastname: Biondi
authors.forename: F
authors.initials: F
authors.lastname: Fossati-Bellani
authors.forename: M
authors.initials: M
authors.lastname: Massimino
mesh_terms: D002648:Child; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007621:Karyotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000532:radiotherapy; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000209:etiology; D016609:Neoplasms, Second Primary; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta