Tex27, a gene containing a zinc-finger domain, is up-regulated during the haploid stages of spermatogenesis.
Tex27 is a gene encoding a protein containing a zinc-finger domain in the carboxy terminal region and a transactivation domain in the amino terminal region. The Tex27 cDNA was isolated from a subtractive library that was enriched for genes preferentially expressed during the development of the seminiferous epithelium. Northern and in situ hybridization analyses demonstrated that Tex27 is differentially expressed in the testis, showing an increased expression in the germ cells corresponding to postmeiotic stages of spermatogenesis. This expression pattern in testis has been described for other C2H2-type zinc-finger proteins in mouse and human, like CTfin51, Zpf29, Sp1, and Zpf37. RFLP-Southern assays revealed that Tex27 is conserved in mammals. The polypeptide analysis and expression pattern suggest that Tex27 is a potential transcription factor preferentially expressed in postmeiotic cells during mouse spermatogenesis.
de Luis
O
O
Departamento de Biología Celular y Desarrollo, Centro de Investigaciones Biológicas (CSIC), Velázquez 144, Madrid, 28006, Spain.
López-Fernández
L A
LA
del Mazo
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J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D019143:Evolution, Molecular; D006238:Haploidy; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; C119731:Tex27 protein, mouse; D014157:Transcription Factors