A novel mechanism for cytokine regulation: screening, selection, and characterization of anticytokine monoclonal and polyclonal autoantibodies.
We have recently described an immunoregulatory mechanism involving release of neutralizing autoantibodies (Aab) to cytokines during bacterial infections. Intraperitoneal inoculation of Haemophilus influenzae type b (Hib) into Sprague-Dawley rats resulted in high levels of inflammatory mediators early after infection. Increased titers of cytokine Aab were observed, with a peak at day 7. We cloned Aab-producing B cells. Screening of the clones with five different cytokines resulted in detection of Aab-producing clones reactive with each cytokine. After repeated subcloning, monoclonal Aab (mAab) were selected and characterized for their specificity, isotypes, and affinities. To elucidate regulatory importance, mAab to interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) dose-dependently inhibited IFN-gamma-induced MHC expression by peritoneal macrophages and TNF-alpha-induced thymocyte proliferation, respectively. Fab fragments exhibited binding and neutralizing effects, confirming specificities. Cross-reactivity with other rat cytokines was excluded. Pools of clones containing several mAab to each cytokine were obtained and served as polyclonal Aab. The relative affinity of the Aab was determined and found to be of high index. The characterized Aab were tested in methodologic assays for cytokine detection, revealing that some Aab were useful in a cell release capturing (CRC) ELISA.
Bakhiet
M
M
Division of Infectious Diseases, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden.
Elkarim
R
R
Diab
A
A
Zhu
J
J
Lindquist
L
L
Link
H
H
Mustafa
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D016207:Cytokines / Q000276:immunology / Q000502:physiology*; D004195:Disease Models, Animal; D015536:Down-Regulation; D006192:Haemophilus Infections / Q000276:immunology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D008403:Mass Screening / Q000379:methods; D016920:Meningitis, Bacterial / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D016207:Cytokines; D018836:Inflammation Mediators