title: Aberrant DNA methylation of the p16INK4a gene in plasma DNA of breast cancer patients.
abstract: Hypermethylation of exon 1 of p16INK4a was examined in tumour and plasma DNA of a series of breast cancer patients. De novo methylation was observed in the tumours of eight patients (23%), and in plasma DNA in five (14%) of these eight patients. Our data show that de novo methylation of exon 1 of p16INK4a can be demonstrated in plasma DNA of breast cancer patients, a fact that provides additional evidence of the tumour-related origin of free plasma DNA in cancer patients.
authors.affiliation: Department of Medical Oncology, Clinica Puerta de Hierro, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Silva
authors.forename: G
authors.initials: G
authors.lastname: Dominguez
authors.forename: M J
authors.initials: MJ
authors.lastname: Villanueva
authors.forename: R
authors.initials: R
authors.lastname: Gonzalez
authors.forename: J M
authors.initials: JM
authors.lastname: Garcia
authors.forename: C
authors.initials: C
authors.lastname: Corbacho
authors.forename: M
authors.initials: M
authors.lastname: Provencio
authors.forename: P
authors.initials: P
authors.lastname: España
authors.forename: F
authors.initials: F
authors.lastname: Bonilla
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D019942:Genes, p16 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D004273:DNA, Neoplasm