Biodegradable alginate microspheres as a delivery system for naked DNA.
Sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. These biodegradable microspheres were used to encapsulate plasmid DNA containing the bacterial beta-galactosidase (LacZ) gene under the control of either the cytomegalovirus (CMV) immediate-early promoter or the Rous sarcoma virus (RSV) early promoter. Mice inoculated orally with microspheres containing plasmid DNA expressed LacZ in the intestine, spleen and liver. Inoculation of mice with microspheres containing both the plasmid DNA and bovine adenovirus type 3 (BAd3) resulted in a significant increase in LacZ expression compared to those inoculated with microspheres containing only the plasmid DNA. Our results suggest that adenoviruses are capable of augumenting transgene expression by plasmid DNA both in vitro and in vivo.
Aggarwal
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N
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA.
HogenEsch
H
H
Guo
P
P
North
A
A
Suckow
M
M
Mittal
S K
SK
D016475:3T3 Cells; D000464:Alginates / Q000493:pharmacokinetics*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001673:Biodegradation, Environmental; D002417:Cattle; D002460:Cell Line; D017690:Cell Transplantation; D003587:Cytomegalovirus / Q000235:genetics; D004337:Drug Carriers; D005822:Genetic Vectors; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D017921:Mastadenovirus; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D011994:Recombinant Proteins; D020723:Glucuronic Acid; D001616:beta-Galactosidase