title: Quantitation of human haptoglobin: comparative ELISA studies using adsorption and capture methods.
abstract: Three ELISA methods for the quantitation of haptoglobin (Hp) in plasma and albumin are described: a polystyrene direct adsorption method and capture methods with antibody and hemoglobin. Hp aggregates generated by 60 degrees C heating showed as much as a hundred-fold higher response by polystyrene adsorption compared to the two capture methods, while unheated Hp showed comparable responses by the three methods.
authors.affiliation: Pharmaceutical Division, Bayer, Berkeley, CA 94701, USA.
authors.forename: E
authors.initials: E
authors.lastname: Yee
authors.forename: J E
authors.initials: JE
authors.lastname: Brown
mesh_terms: D000327:Adsorption; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006242:Haptoglobins / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D012015:Reference Standards
chemical_list: D006242:Haptoglobins