title: Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae.
abstract: House dust mite allergen is thought to be a major cause of asthma. Characterization of these allergen molecules is therefore an important step for the development of effective diagnostic and therapeutic agents against mite-associated allergic disorders. Here we report molecular cloning and expression of the group 6 (chymotrypsin-like) allergen from the house dust mite, Dermatophagoides farinae. Sequencing analysis indicates that cloned cDNA, designated Der f 6, encodes a 279 amino acid polypeptide which conserves a primary structure characteristic for chymotrypsin-like serine proteases found in mammals. Recombinant Der f 6 expressed in Escherichia coli bound IgE in a pool made of 20 sera, and induced histamine release from patients' peripheral blood cells.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan. skawa@ipc.hiroshima-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuguchi
authors.forename: K
authors.initials: K
authors.lastname: Morimoto
authors.forename: T
authors.initials: T
authors.lastname: Aki
authors.forename: S
authors.initials: S
authors.lastname: Shigeta
authors.forename: H
authors.initials: H
authors.lastname: Yasueda
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: O
authors.initials: O
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Jyo
authors.forename: K
authors.initials: K
authors.lastname: Ono
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D004391:Dust; D004926:Escherichia coli / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D004391:Dust; D006023:Glycoproteins; D012697:Serine Endopeptidases