title: Dendritic cell-T-cell interactions support coreceptor-independent human immunodeficiency virus type 1 transmission in the human genital tract.
abstract: Worldwide, human immunodeficiency virus (HIV) is transmitted predominantly by heterosexual contact. Here, we investigate for the first time, by examining mononuclear cells obtained from cervicovaginal tissue, the mechanisms whereby HIV type 1 (HIV-1) directly targets cells from the human genital tract. In contrast to earlier findings in mucosal models such as human skin, we demonstrate that the majority of T cells and macrophages but none or few dendritic cells (DC) express the HIV-1 coreceptor CCR5 in normal human cervicovaginal mucosa, whereas all three cell types express the coreceptor CXCR4. To understand the role of coreceptor expression on infectivity, mucosal mononuclear cells were infected with various HIV-1 isolates, using either CCR5 or CXCR4. Unstimulated T cells become rapidly, albeit nonproductively, infected with R5- and X4-tropic variants. However, DC and T cells form stable conjugates which permit productive infection by viruses of both coreceptor specificities. These results indicate that HIV-1 can exploit T-cell-DC synergism in the human genital tract to overcome potential coreceptor restrictions on DC and postentry blocks of viral replication in unactivated T cells. Thus, mononuclear cells infiltrating the genital mucosa are permissive for transmission of both R5- and X4-tropic HIV-1 variants, and selection of virus variants does not occur by differential expression of HIV-1 coreceptors on genital mononuclear cells.
authors.affiliation: Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: F
authors.initials: F
authors.lastname: Hladik
authors.forename: G
authors.initials: G
authors.lastname: Lentz
authors.forename: R E
authors.initials: RE
authors.lastname: Akridge
authors.forename: G
authors.initials: G
authors.lastname: Peterson
authors.forename: H
authors.initials: H
authors.lastname: Kelley
authors.forename: A
authors.initials: A
authors.lastname: McElroy
authors.forename: M J
authors.initials: MJ
authors.lastname: McElrath
mesh_terms: D002584:Cervix Uteri / Q000166:cytology / Q000821:virology; D003713:Dendritic Cells / Q000378:metabolism* / Q000821:virology; D005260:Female; D005836:Genitalia, Female / Q000166:cytology / Q000821:virology*; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D009092:Mucous Membrane / Q000166:cytology; D010641:Phenotype; D019713:Receptors, CCR5 / Q000096:biosynthesis*; D019718:Receptors, CXCR4 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D014621:Vagina / Q000166:cytology / Q000821:virology; D014779:Virus Replication
chemical_list: D019713:Receptors, CCR5; D019718:Receptors, CXCR4