title: Reconstitution of the actin-scavenger system after orthotopic liver transplantation for end-stage liver disease: a prospective and longitudinal study.
abstract: Serum levels of the actin scavenger Gc-globulin (group-specific component, vitamin D-binding protein), a member of the albumin multigene family, are decreased in severe liver disease but have not been evaluated in relation to liver transplantation. We measured Gc-globulin and Gc-globulin-actin complex ratio daily for 2 weeks after transplantation in 17 patients with end-stage liver disease. Before transplantation, Gc-globulin levels were significantly less in the patients than in healthy controls (235 +/- 106 v 340 +/- 35 mg/L, respectively; P<.001), whereas complex ratio level was in the normal range. Five patients (group N) had pretransplantation Gc-globulin values within the normal range (mean +/- 2 SD), and 12 patients had subnormal values (group S). In group N, mean Gc-globulin levels posttransplantation remained stable at a lower level than before transplantation but still within normal range. In this group, cold ischemia time correlated inversely with Gc-globulin levels on day 2 (r = -0.88; P <.05). In group S, normal mean levels were reached at a mean of 11 days after transplantation. However, almost half these patients had subnormal Gc-globulin levels at day 14. Complex ratio levels remained normal in the study period in both groups. Prothrombin index levels (plasma coagulation factors II, VII, and X) were identical in both groups and returned to normal 7 days posttransplantation, whereas plasma albumin levels were less than normal in both groups and further decreased after transplantation. In conclusion, the maintenance (group N) or reestablishment (group S) of serum Gc-globulin to normal levels occurred in the early posttransplantation course in the same time frame as the prothrombin index. Gc-globulin synthesis seems unrelated to albumin synthesis. A prolonged cold ischemia time may cause reduced Gc-globulin levels early after transplantation.
authors.affiliation: Department of Hepatology, Rigshospitalet, Copenhagen, Denmark.
authors.forename: F V
authors.initials: FV
authors.lastname: Schiodt
authors.forename: S
authors.initials: S
authors.lastname: Bondesen
authors.forename: K
authors.initials: K
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Rasmussen
authors.forename: A
authors.initials: A
authors.lastname: Hjortrup
authors.forename: P
authors.initials: P
authors.lastname: Kirkegaard
authors.forename: B A
authors.initials: BA
authors.lastname: Hansen
authors.forename: N
authors.initials: N
authors.lastname: Tygstrup
authors.forename: P
authors.initials: P
authors.lastname: Ott
mesh_terms: D000199:Actins / Q000378:metabolism*; D000328:Adult; D015925:Cryopreservation; D005167:Factor VII / Q000032:analysis; D005170:Factor X / Q000032:analysis; D005260:Female; D006801:Humans; D017093:Liver Failure / Q000378:metabolism / Q000601:surgery*; D016031:Liver Transplantation / Q000379:methods / Q000502:physiology*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D011516:Prothrombin / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors; D014809:Vitamin D-Binding Protein / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics
chemical_list: D000199:Actins; D012709:Serum Albumin; D014809:Vitamin D-Binding Protein; D005167:Factor VII; D011516:Prothrombin; D005170:Factor X