title: Telomere dynamics in monkeys: increased cell turnover in macaques infected with chimeric simian-human immunodeficiency viruses.
abstract: To address the question of how cell turnover is affected by retroviral infections, we used the telomeric terminal restriction fragments (TRFs) as markers of cell replicative history and measured their length in macaques infected with chimeric simian-human immunodeficiency viruses (SHIVs). The TRF lengths of mononuclear cells in 104 samples, including longitudinal samples from nine cynomolgus and ten pig-tailed macaques infected with SHIV, and in samples from 26 uninfected macaques, were quantitated by an improved method, based on two-dimensional calibration of DNA sizes, pulsed field electrophoresis, and high-resolution Southern blot images. The average TRF lengths of peripheral blood mononuclear cells (PBMCs) from uninfected pig-tailed (14.9+/-1.6 kbp) and cynomolgus (14.1+/-1.8 kbp) macaques were about 3 and 5 kbp longer than those of human infants and 30-year-old adults, respectively. The rate of TRF length shortening in infected pig-tailed macaques was significantly (P = 0.035) higher (2.2-fold) than in uninfected monkeys. The TRFs in SHIV-infected cynomolgus monkeys, which, in general, had lower viral loads than pig-tailed macaques, shortened on average more rapidly (1.6-fold) than in uninfected animals, but the difference was not statistically significant. The TRFs of mononuclear cells from the lymph nodes of two rapidly progressing SHIV-infected macaques that developed AIDS and died also shortened in parallel but somewhat more rapidly than in the PBMCs. These results suggest that the rate of PBMC turnover in macaques could be increased several-fold during infections by immunodeficiency viruses, likely due to immune activation by SHIV antigens.
authors.affiliation: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA.
authors.forename: R
authors.initials: R
authors.lastname: Shibata
authors.forename: Y R
authors.initials: YR
authors.lastname: Feng
authors.forename: D
authors.initials: D
authors.lastname: Gee
authors.forename: D
authors.initials: D
authors.lastname: Norwood
authors.forename: X
authors.initials: X
authors.lastname: Xiao
authors.forename: S L
authors.initials: SL
authors.lastname: Zeichner
authors.forename: M A
authors.initials: MA
authors.lastname: Martin
authors.forename: D S
authors.initials: DS
authors.lastname: Dimitrov
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics*; D002678:Chimera; D015658:HIV Infections / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008251:Macaca / Q000033:anatomy & histology / Q000235:genetics*; D008252:Macaca fascicularis / Q000033:anatomy & histology / Q000235:genetics; D008253:Macaca mulatta / Q000033:anatomy & histology / Q000235:genetics; D008254:Macaca nemestrina / Q000033:anatomy & histology / Q000235:genetics; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000473:pathology*; D016615:Telomere / Q000235:genetics*; D013997:Time Factors