title: Prevalence of calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity.
abstract: One hundred and seventeen rectal fecal specimens were collected in 1995 and 1996 from calves with diarrhea in Kagoshima Prefecture in Japan. The bovine group A rotavirus was detected by enzyme immunoassay in 43 of 117 specimens and isolated from 33 of the 43 specimens that were positive. G serotype, P serotype, and P genotype of 33 isolates were identified by reverse transcription-polymerase chain reaction, and 20 of 33 isolates (60.6%) were identified as G serotype 8. Thus, we discovered that calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity was prevalent in this research area during this research period. To our knowledge, this is the first report on the prevalence of calf diarrhea caused by the bovine group A rotavirus carrying G serotype 8 specificity.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukai
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Itou
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers / Q000737:chemistry; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D007564:Japan / Q000453:epidemiology; D015995:Prevalence; D012367:RNA, Viral / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000472:pathogenicity*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012835:Silver Nitrate / Q000737:chemistry; D016622:Silver Staining / Q000662:veterinary
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D012835:Silver Nitrate