title: Effects of lysophosphatidic acid on proliferation and cytosolic Ca++ of human adult vascular smooth muscle cells in culture.
abstract: Lysophosphatidic acid (LPA) is a lipid mediator generated by activated platelets and having various effects on numerous cell types. We investigated some effects of 1-oleyl LPA on vascular smooth muscle cells cultured from adult human normal arteries. At micromolar concentrations, LPA induced a mitogenic effect ([3H]-thymidine incorporation and cell proliferation) on quiescent cells, without an additional growth factor being required. This effect was equipotent to that of 10% fetal calf serum, and it was accompanied by early (5 minutes) and late (1-3 hours) phosphorylation of mitogenactivated protein kinase. LPA inhibited cell migration through collagen coated membranes, with or without platelet-derived growth factor BB as chemoattractant. LPA induced a typical biphasic Ca2+ signal response made up of a rapid first phase due to Ca2+ release from intracellular stores followed by a second wave due to external Ca2+ influx. These findings support the proposal that LPA released from activated platelets is a mediator for smooth muscle cell response at the site of vessel injury in humans.
authors.affiliation: Service d'Hématologie, Fac. Pharmacie, Toulouse, France.
authors.forename: I
authors.initials: I
authors.lastname: Gennero
authors.forename: J M
authors.initials: JM
authors.lastname: Xuereb
authors.forename: M F
authors.initials: MF
authors.lastname: Simon
authors.forename: J P
authors.initials: JP
authors.lastname: Girolami
authors.forename: J L
authors.initials: JL
authors.lastname: Bascands
authors.forename: H
authors.initials: H
authors.lastname: Chap
authors.forename: B
authors.initials: B
authors.lastname: Boneu
authors.forename: P
authors.initials: P
authors.lastname: Sié
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002450:Cell Communication; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011460:Prostaglandins F / Q000494:pharmacology
chemical_list: D008246:Lysophospholipids; D011460:Prostaglandins F; D002118:Calcium