Isolation and characterization of a sigB deletion mutant of Staphylococcus aureus.
The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mutant showed no reduction in virulence.
Nicholas
R O
RO
SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania, USA.
Li
T
T
McDevitt
D
D
Marra
A
A
Sucoloski
S
S
Demarsh
P L
PL
Gentry
D R
DR
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D017353:Gene Deletion; D051379:Mice; D051381:Rats; D012808:Sigma Factor / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; C079011:SigB protein, Bacteria; D012808:Sigma Factor