Detection of exon skipping and retained introns in transcription of the human thrombospondin 2 gene.
Alternative transcripts of the gene for human thrombospondin 2 (TSP2), an extracellular glycoprotein, have been identified using a PCR procedure. Species that show exon skipping and/or retained introns were revealed. In particular, a species was characterized in which exon 3 has been spliced from the sequence. This transcript is otherwise intact from exon 1A to exon 22. Transcripts which retain intron 1A, intron 1B, or intron 2 were also identified. Tissue-specific differences were evident in the transcripts with retained introns. In addition, a species which both retained intron 1B and skipped exon 3 was revealed. The existence of these alternative transcripts of the human TSP2 gene could be important for understanding the diverse functions and tissue-specific expression implicated for TSP2.
Adolph
K W
KW
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, 55455, USA.
D017398:Alternative Splicing; D001483:Base Sequence; D001921:Brain / Q000196:embryology; D005091:Exons; D006801:Humans; D007438:Introns; D008957:Models, Genetic; D008969:Molecular Sequence Data; D019699:Thrombospondins / Q000235:genetics*; D014158:Transcription, Genetic; D000099262:Thrombospondin 2
D019699:Thrombospondins; D000099262:Thrombospondin 2