Characterization of two monoclonal antibodies against porcine VCAM-1.
Immunization of mice with TNF alpha-activated porcine endothelial cells led to the characterization of two monoclonal antibodies (MAbs), 5F3 and 8A7, specific for porcine VCAM-1. Upon flow cytometry, both antibodies increasingly labeled endothelial cells according to their degree of activation. They bound a band of MW 80 kDa on Western blots of endothelial cells, which is the apparent molecular weight of porcine VCAM-1. It was determined by surface plasmon resonance that the antibodies are directed to different antigenic sites. It was also found that 5F3 competes for binding the antigen with a MAb previously characterized as binding domain 1 of porcine VCAM-1. Subsequently, 5F3, but not 8A7, was found to inhibit the adhesion of human B lymphocyte Ramos cells to porcine endothelial cells in vitro. These antibodies, which do not cross-react with human VCAM-1, might be useful for diagnostic or therapeutic purposes in xenotransplantation.
Richard
C
C
INSERM U437, Institut de Transplantation Et de Recherche en Transplantation, Centre Hospitalier Universitaire de Nantes, France.
Charreau
B
B
Vusio
P
P
Soulillou
J P
JP
Bouhours
J F
JF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D000918:Antibody Specificity; D001011:Aorta / Q000166:cytology; D001402:B-Lymphocytes / Q000187:drug effects; D015374:Biosensing Techniques; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003429:Cross Reactions; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D013045:Species Specificity; D013552:Swine / Q000276:immunology*; D014183:Transplantation, Heterologous; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1