title: Insecticidal activity of an alpha-amylase inhibitor-like protein resembling a putative precursor of alpha-amylase inhibitor in the common bean, Phaseolus vulgaris L.
abstract: alpha-Amylase inhibitor (alphaAI) in the common bean, Phaseolus vulgaris L., protects seeds from insect pests such as the cowpea weevil (Callosobruchus maculatus) and the azuki bean weevil (C. chinensis). Cultivars which lack alphaAI still show resistance to both bruchids. These cultivars have a glycoprotein that reacts with anti-alphaAI-1 antibodies. The glycoprotein with a molecular mass of 29 kDa (Gp29) was purified and the encoding gene was isolated. The primary structure of Gp29 is the same as alpha-amylase inhibitor-like protein (AIL) from which the encoding gene has already been isolated. AIL resembles a putative precursor of alphaAI, even though it does not form the active inhibitor. However, AIL has some inhibitory effect on the growth of C. maculatus but not C. chinensis. The presence of AIL alone is insufficient to explain the bruchid resistance of common bean cultivars lacking alpha-AI. Common bean seeds appear to contain several factors responsible for the bruchid resistance.
authors.affiliation: Legume Breeding Laboratory, National Agriculture Research Center, Tsukuba, Ibaraki 305-8666, Japan. ishimoto@narc.affrc.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Ishimoto
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Kaga
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D015723:Gene Library; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006386:Hemagglutination Tests; D007306:Insecticides / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment
chemical_list: D006023:Glycoproteins; D007306:Insecticides; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D011498:Protein Precursors; C061510:alpha-amylase inhibitor, Phaseolus vulgaris; C120230:alpha-amylase inhibitor-like protein, Phaseolus vulgaris; C110154:arcelin-1