title: Identification of two new proteins in spermidine nucleoids isolated from Escherichia coli.
abstract: The Escherichia coli nucleoid contains DNA in a condensed but functional form. Analysis of proteins released from isolated spermidine nucleoids after treatment with DNase I reveals significant amounts of two proteins not previously detected in wild-type E. coli. Partial amino-terminal sequencing has identified them as the products of rdgC and yejK. These proteins are strongly conserved in gram-negative bacteria, suggesting that they have important cellular roles.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0560, USA.
authors.forename: L D
authors.initials: LD
authors.lastname: Murphy
authors.forename: J L
authors.initials: JL
authors.lastname: Rosner
authors.forename: S B
authors.initials: SB
authors.lastname: Zimmerman
authors.forename: D
authors.initials: D
authors.lastname: Esposito
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017124:Conserved Sequence; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D006090:Gram-Negative Bacteria / Q000737:chemistry; D008969:Molecular Sequence Data; D015388:Organelles / Q000737:chemistry*; D013095:Spermidine / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C103478:RdgC protein, E coli; C119943:YejK protein, E coli; D003850:Deoxyribonuclease I; D013095:Spermidine