[Study of transcription and cleavage in vitro of HDV with HBV-specific hammerhead ribozyme].
OBJECTIVE
To study the effect of hepatitis B virus(HBV) specific ribozyme(RZ) and recombinant hepatitis D virus(HDV) inserting hammerhead ribozyme(rHDVRZP and rHDVRZA).


METHODS
831 bp HBV C gene fragment was cloned under the control of T7 promoter, 32P-labeled HBV transcript was incubated with gel-purified RZ, rHDVRZA, rHDVRZP at different temperature and autoradiographed after denaturing gel-electrophoresis.


RESULTS
These results show that rRZ, rHDVRZA, rHDVRZP were active at 37 degrees C and more so at higher temperatures.


CONCLUSIONS
Recombinant Delt virus could serve as a vector for the delivery of a ribozyme specific for hepatitis B virus cleavage. Our data demonstrate the value of recombinant ribozyme as potential therapeutic agents for treatment of HBV infection. Further study about cleavage in vitro and in vivo will continue.
Wen
S
S
Genetic Centre, Nanfang Hospital, Guangzhou.
Qi
X
X
Zhou
R
R
D003001:Cloning, Molecular; D006515:Hepatitis B virus / Q000235:genetics*; D003698:Hepatitis Delta Virus / Q000235:genetics*; D016337:RNA, Catalytic / Q000502:physiology*; D013696:Temperature; D014158:Transcription, Genetic
D016337:RNA, Catalytic