Neuronal actions of oxytocin on the subfornical organ of male rats.
The aim of this study was to investigate effects of oxytocin (OT) on electrical neuronal activities in rat subfornical organ (SFO) and compare its action with the well-described excitatory effects of blood-borne angiotensin II (ANG II) on the same SFO neurons. With the use of extracellular recordings from spontaneously active neurons in slice preparations of the SFO of male rats, 11.7% of tested neurons (n = 206) were excited and 9.7% were inhibited by superfusion with 10(-6) M OT. Both excitatory and inhibitory effects of OT were dose dependent with similar threshold concentrations and were blocked by a specific OT-receptor antagonist but not by a vasopressin receptor antagonist. Blocking synaptic transmission with low calcium medium suppressed only inhibitory effects of OT. All but one of the OT-sensitive neurons were also excited by superfusion with ANG II at a concentration much lower than required for OT, suggesting that synaptically released OT rather than blood-borne OT alters the activity of SFO neurons in vivo. The results support the hypothesis that neurally released OT may modulate SFO-mediated functions by acting on OT-sensitive neurons.
Hosono
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Department of Physiology, School of Allied Health Sciences, Osaka University Faculty of Medicine, Suita, Osaka 565-0871, Japan. hosono@sahs.med.osaka-u.ac.jp
Schmid
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HA
Kanosue
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Simon
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D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000008:administration & dosage; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D066298:In Vitro Techniques; D008297:Male; D009433:Neural Inhibition / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D010121:Oxytocin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018045:Receptors, Oxytocin / Q000037:antagonists & inhibitors; D013356:Subfornical Organ / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects
D003470:Culture Media; D018045:Receptors, Oxytocin; D000804:Angiotensin II; D010121:Oxytocin; D002118:Calcium