Cytotoxic activity of pierisin, from the cabbage butterfly, Pieris rapae, in various human cancer cell lines.
Pierisin, a protein purified from pupae of the cabbage butterfly, Pieris rapae, exhibits cytotoxic effects against the human gastric cancer TMK-1 cell line, inducing apoptosis. The present study was performed to determine whether pierisin might exert a similar influence on nine other human cancer cell lines and human umbilical vein endothelial cells (HUVECs). Pierisin showed cytotoxic effects in all the human cells tested, with IC50 values ranging from 0.043 ng/ml to 150 ng/ml. Among the target cells, the cervical carcinoma cell line, HeLa, was the most sensitive to pierisin, showing a 1000-fold less IC50 value than that of HUVECs. While pierisin clearly induced apoptotic cell death in most cancer cell lines and HUVECs, the pathway appeared to be probably different from that involving anti-Fas, TNF-alpha and p53. Pierisin may thus be a promising new candidate for cancer therapy.
Kono
T
T
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan.
Watanabe
M
M
Koyama
K
K
Kishimoto
T
T
Fukushima
S
S
Sugimura
T
T
Wakabayashi
K
K
D036002:ADP Ribose Transferases; D000818:Animals; D017209:Apoptosis; D002080:Butterflies / Q000737:chemistry; D003603:Cytotoxins / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D019476:Insect Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003603:Cytotoxins; D019476:Insect Proteins; C113557:pierisin protein, insect; D036002:ADP Ribose Transferases