title: [Effect of the RNAase from Bacillus intermedius on growth and physiological characteristics of Escherichia coli].
abstract: The effect of the RNase from Bacillus intermedius on the growth of Escherichia coli was investigated. RNase added to growth medium enhanced the synthesis of DNA, RNA, and protein and stimulated cell division; the degree of stimulation depended on the enzyme concentration. A necessary condition for stimulation was the adsorption of the enzyme on the cell surface and its interaction with the cytoplasmic membrane, as demonstrated immunocytochemically. The adsorption of the enzyme was accompanied by a 43% decrease in the surface charge density. Other effects of RNase involved a 25% increase in the growth rate, a 38% biomass gain, and generation time shortening by 10 min. The stimulation of bacterial growth correlated with the stimulation of cellular respiration rate.
authors.affiliation: Kazan State University, Tatarstan, Russia.
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Egorov
authors.forename: S V
authors.initials: SV
authors.lastname: Ulakhovich
authors.forename: V V
authors.initials: VV
authors.lastname: Dmitriev
authors.forename: F G
authors.initials: FG
authors.lastname: Kupriianova-Ashina
authors.forename: I B
authors.initials: IB
authors.lastname: Leshchinskaia
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D008564:Membrane Potentials; D012260:Ribonucleases / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012260:Ribonucleases
vernacular_title: Issledavanie vliianiia RNKazy Bacillus intermedius na rost i fiziologicheskuiu kharakteristiku Esherichia coli.