Effects of lung preservation solutions on PMN activation in vitro.
Polymorphonuclear leukocyte (PMN) activation and PMN-endothelial cell interactions may cause graft failure due to ischemia-reperfusion injury after lung transplantation. We investigated the effects of Euro-Collins solution (EC), low-potassium dextran solution (LPD), and EC plus pentoxifylline (EC-PTXF) on adhesion molecule (CD11b/CD18 and L-selectin) expression, chemotaxis, and oxidative burst of PMN. PMN from healthy human volunteers were incubated with EC, LPD, and EC-PTXF, and, in controls, without preservation solution. LPD exerted a suppressive effect on PMN chemotaxis as compared to EC (P < 0.05), but had no attenuating effect on the increase of CD11b/CD18, the shedding of L-selectin, and intracellular oxidant generation. EC-PTXF attenuated the expression of CD11b/CD18 and the oxidative burst as compared to EC alone (P < 0.05). These effects of LPD and PTXF on PMN function may contribute to successful organ preservation in transplantation.
Sakamaki
F
F
Department of medicine, National Cardiovascular Center, Osaka, Japan.
Hoffmann
H
H
Münzing
S
S
Krombach
F
F
Messmer
K
K
Schildberg
F W
FW
D018821:CD18 Antigens / Q000235:genetics; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003574:Cytochrome c Group / Q000378:metabolism; D003911:Dextrans / Q000494:pharmacology; D005786:Gene Expression Regulation; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D066298:In Vitro Techniques; D019041:L-Selectin / Q000235:genetics; D008168:Lung; D016177:Macrophage-1 Antigen / Q000235:genetics; D018375:Neutrophil Activation / Q000187:drug effects* / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010431:Pentoxifylline / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000097:blood
D018821:CD18 Antigens; D003574:Cytochrome c Group; D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D016177:Macrophage-1 Antigen; D019188:Organ Preservation Solutions; C083290:low potassium dextran glucose solution; D013481:Superoxides; D019041:L-Selectin; D005947:Glucose; D010431:Pentoxifylline