Human papilloma virus DNA detection in oral lesions in the Greek population.
HPVs (Human Papilloma Viruses) are small DNA "epitheliotropic" viruses, implicated in cervical carcinogenesis, particularly the "high-oncogenic-risk" types HPV-16 and HPV-18. Data concerning oral carcinogenesis are however, contradictory. We examined the presence of HPV and subsequently HPV-16 and HPV-18 in 102 specimens of paraffin-embedded oral tissue blocks--81 oral squamous cell carcinomas and 21 oral hyperplasias--using PCR technique followed by agarose gel electrophoresis. Our results demonstrated that 49% (50/102) of the samples were HPV positive. Subsequent analysis of HPV positive lesions revealed 22% positivity for HPV-16 and 44% for HPV-18. HPV-18 was detected only in carcinomas, while HPV-16 was more abundant in papillomatous hyperplasias and in a small percentage of carcinomas. These findings may probably indicate a contributing role for HPV-18 as a potent co-carcinogen in oral epithelial carcinogenesis in the Greek population.
Aggelopoulou
E P
EP
Medical School of Athens, Department of Histology-Embryology, Greece.
Skarlos
D
D
Papadimitriou
C
C
Kittas
C
C
Troungos
C
C
D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000382:microbiology*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006115:Greece; D006801:Humans; D006965:Hyperplasia; D008297:Male; D009055:Mouth / Q000382:microbiology* / Q000473:pathology; D009062:Mouth Neoplasms / Q000453:epidemiology / Q000382:microbiology*; D015513:Oncogene Proteins / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012189:Retrospective Studies; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000382:microbiology*
D017931:DNA Primers; D004279:DNA, Viral; C093478:E1 protein, Human papillomavirus 16; D015513:Oncogene Proteins; D009856:Oncogene Proteins, Viral; C084762:oncogene protein E1, Human papillomavirus type 18