Effects of ELF capacitively coupled weak electric fields on metabolism of 6B1 cells.
In this study, we adopted several methods of MTT colorimetry, DAPI fluorimetry and ELISA to study the effects of extremely low frequency (ELF) capacitively coupled electric fields (EFs) on the metabolism of 6B1 cells. The result shows that 50 mV cm(-1) ELF EF (10-100 Hz) has no significant effect on proliferation, DNA synthesis and activity of succinate dehydrogenase of 6B1 cells, indicating that the effect of ELF (10-100 Hz) EF on the metabolism of 6B1 cells is not obvious. However, 50 mV cm(-1), 50 Hz EF significantly promotes the HBs-Ab (Hepatitis B surface antibody) secretion of 6B1 cells, implying that under this situation, EF has some distinctive effect on the outerface of 6B1 cell membrane.
Wang
X
X
Department of Biophysical Science and Technology, Nankai University, Tianjin, China.
Zhou
A
A
Liu
M
M
Yu
H
H
Pang
L
L
Zhu
M
M
Wang
L
L
Berg
H
H
D002455:Cell Division; D002460:Cell Line; D003124:Colorimetry; D004560:Electricity; D004797:Enzyme-Linked Immunosorbent Assay; D005470:Fluorometry; D008928:Mitochondria / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase