Characterization of several LINE-1 elements in Microtus kirgisorum.
Several LINE-1s have been isolated and characterized from genomic DNA of the vole, Microtus kirgisorum. Blot hybridization revealed specific restriction patterns of L1 elements in vole genomes. Rehybridization of the genomic blot with a cloned 5'-end fragment revealed two major bands indicating the presence of two different L1 subfamilies. The copy numbers are estimated for different parts of M. kirgisorum L1 elements. Data also demonstrate that most vole L1 elements are truncated at the 5'-end; however, in contrast to mouse, the ORF1 copy number is higher in vole. A difference between the substitution rates of the ORF1 5'-region (approximately 330 nucleotides) and the rest of the L1 coding regions is revealed.
Mayorov
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Division of Basic Medical Sciences, Mercer University School of Medicine, 1550 College Street, Macon, Georgia 31207, USA.
Rogozin
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Adkison
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LR
D000595:Amino Acid Sequence; D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D001483:Base Sequence; D016386:Chromosome Walking; D004247:DNA; D020084:Long Interspersed Nucleotide Elements; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004247:DNA