Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae).
The genetic variability of seven European corn borer populations, Ostrinia nubilalis, from North America and Europe was assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing. The nuclear ribosomal internal transcribed spacer 1 (ITS-1) region (approximately 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The smartweed borer, Ostrinia obumbratalis, and south-Western corn borer, Diatraea grandiosella, were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS-1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The lack of variation in the ITS-1 of European corn borer was confirmed by DNA sequence analysis. The genetic similarity of European corn borer populations, despite their wide geographical range and physiological differences, may be explained by a relatively recent origin for the voltinism and pheromone races, gene flow among races, and/or expansion from genetic bottlenecks.
Marçon
P C
PC
Department of Entomology, University of Nebraska-Lincoln, 68583-0816, USA.
Taylor
D B
DB
Mason
C E
CE
Hellmich
R L
RL
Siegfried
B D
BD
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D005260:Female; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D010675:Pheromones / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020033:Protein Isoforms; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D019476:Insect Proteins; D010675:Pheromones; D020033:Protein Isoforms