Cutting edge: expression of the NF of activated T cells in eosinophils: regulation by IL-4 and IL-5.
We report that NF-AT1 and NF-AT4 are expressed cytoplasmically in resting eosinophils, whereas NF-AT2 and NF-AT3 have not been seen. Likewise, NF-AT1 mRNA and NF-AT4 mRNA have been detected in resting eosinophils, and their levels can be significantly up-regulated by the Th2-associated cytokines IL-4 and IL-5. There is no detectable NF-AT protein expression in the nuclei of resting eosinophils. However NF-ATs appear in the nuclei of IL-4-, IL-5-, or ionomycin-stimulated eosinophils. Only NF-AT1 and NF-AT4, but not NF-AT2 and NF-AT3, have translocated into the nuclei in IL-4- or IL-5-stimulated eosinophils. These findings delineate a novel pathway in the cytokine network in which Th2 lymphocytes "control" eosinophils via the release of IL-4 and IL-5, and activation of NF-AT in eosinophils. The findings also suggest that a later feedback "talking" may exist between eosinophils and Th2 lymphocytes.
Jinquan
T
T
Laboratory of Medical Allergology, Allergy Unit, Department of Clinical Immunology, National University Hospital, Copenhagen, Denmark. TAN@RH.DK
Quan
S
S
Jacobi
H H
HH
Reimert
C M
CM
Millner
A
A
Hansen
J B
JB
Thygesen
C
C
Ryder
L P
LP
Madsen
H O
HO
Malling
H J
HJ
Poulsen
L K
LK
D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics / Q000276:immunology; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D015847:Interleukin-4 / Q000502:physiology*; D015848:Interleukin-5 / Q000502:physiology*; D008213:Lymphocyte Activation; D005810:Multigene Family / Q000276:immunology; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics / Q000276:immunology
D004268:DNA-Binding Proteins; D015848:Interleukin-5; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D015847:Interleukin-4