title: Diagnosis of nosocomial pneumonia in cancer patients undergoing mechanical ventilation: a prospective comparison of the plugged telescoping catheter with the protected specimen brush.
abstract: OBJECTIVE
  Quantitative culture of protected samples of lower respiratory tract secretions obtained by a fiberoptic protected specimen brush (PSB) is widely accepted for the diagnosis of ventilator-associated pneumonia (VAP), but this diagnostic procedure is time consuming, expensive, and may give rise to iatrogenic complications, especially in cancer patients who often present with thrombocytopenia. The plugged telescoping catheter (PTC) could be a satisfactory alternative to the PSB in this setting. The aim of the present study was to evaluate the interest of the PTC to diagnose VAP in ventilated cancer patients.
  
  
  METHODS
  A prospective observational study.
  
  
  METHODS
  A 15-bed medical-surgical ICU in a comprehensive cancer center.
  
  
  METHODS
  Over a 9-month period, 42 patients suspected of having bacterial VAP during mechanical ventilation underwent 69 bronchial samplings: a blinded PTC and a fiberoptic PSB were performed successively in each case. A positive culture for both sampling procedures was defined as the recovery of > or = 10(3) cfu/mL of at least one potential pathogen. The PSB result was taken as the reference standard.
  
  
  RESULTS
  The overall agreement between the techniques was 87% (60/69). PTC had a sensitivity of 67%, a specificity of 93%, a positive predictive value of 71%, and a negative predictive value of 91%.
  
  
  CONCLUSIONS
  We conclude that the accuracy of the blinded PTC compares well with that of the PSB for the diagnosis of VAP in cancer patients. The sensitivity of the PTC observed herein, which is slightly lower than that described in previous studies, may be due to the blinded nature of the method: the indications for initial or secondary coupling with a directed sampling method in patients with suspicion of localized pneumonia remain to be determined.
authors.affiliation: Intensive Care Unit, Institut Gustave Roussy, Villejuif, France.
authors.forename: M
authors.initials: M
authors.lastname: Casetta
authors.forename: F
authors.initials: F
authors.lastname: Blot
authors.forename: S
authors.initials: S
authors.lastname: Antoun
authors.forename: B
authors.initials: B
authors.lastname: Leclercq
authors.forename: C
authors.initials: C
authors.lastname: Tancrède
authors.forename: F
authors.initials: F
authors.lastname: Doyon
authors.forename: G
authors.initials: G
authors.lastname: Nitenberg
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001999:Bronchoscopy; D003428:Cross Infection / Q000175:diagnosis* / Q000209:etiology; D004866:Equipment Contamination; D005260:Female; D005336:Fiber Optic Technology; D006801:Humans; D007442:Intubation, Intratracheal / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy*; D015588:Observer Variation; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000209:etiology; D011446:Prospective Studies; D012121:Respiration, Artificial / Q000009:adverse effects*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation*