Effects of tanshinone II-A sulfonate on adhesion molecule expression of endothelial cells and platelets in vitro.
OBJECTIVE
To study the action of tanshinone II-A sulfonate (Tan) on adhesion molecule expression by cultured endothelial cells and platelets.


METHODS
Tumor necrosis factor alpha (TNF-alpha)-induced ICAM-1 expression on the cell surface and endothelial adhesivity toward HL-60 cells were studied using human umbilical vein endothelial cells (HUVEC). Thrombin-induced expression of platelet P-selectin was studied using human blood platelets. Adhesion molecule expression on the cell surface was measured by flow cytometry. The number of HL-60 cells adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy.


RESULTS
Pretreatment of HUVEC with TNF-alpha significantly enhanced ICAM-1 expression and increased HL-60 cells adhesion to HUVEC from 4.6% +/- 0.7% to 30% +/- 6%. Tan (25-200 mumol.L-1) inhibited the effects of TNF-alpha in a concentration-dependent manner. Tan also inhibited the increase of P-selectin expression of thrombin-activated platelets in a concentration-dependent manner.


CONCLUSIONS
Tan inhibited expression of adhesion molecules (ICAM-1, P-selectin) in HUVEC and in human blood platelets.
Jiang
K Y
KY
Department of Pharmacology, Suzhou Medical College, China.
Ruan
C G
CG
Gu
Z L
ZL
Zhou
W Y
WY
Guo
C Y
CY
D001792:Blood Platelets / Q000378:metabolism*; D002317:Cardiovascular Agents / Q000494:pharmacology; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology*; D018922:HL-60 Cells / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D019007:P-Selectin / Q000096:biosynthesis*; D010616:Phenanthrenes / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D019007:P-Selectin; D010616:Phenanthrenes; D018799:Intercellular Adhesion Molecule-1; C024894:tanshinone II A sodium sulfonate