Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.
Shi
S H
SH
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Hayashi
Y
Y
Petralia
R S
RS
Zaman
S H
SH
Wenthold
R J
RJ
Svoboda
K
K
Malinow
R
R
D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D004558:Electric Stimulation; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D006801:Humans; D017774:Long-Term Potentiation; D009473:Neuronal Plasticity; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D011940:Receptor Aggregation; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission; D013746:Tetany
D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins