title: Placental Fas ligand expression is a mechanism for maternal immune tolerance to the fetus.
abstract: Fas ligand (FasL) is a peptide that plays an important immunoregulatory role in limiting the host immune response. Several studies have shown that the expression of FasL in the anterior chamber of the eye and the testis allows these tissues to be immunoprivileged sites. Immunotolerance is achieved by binding of FasL to its receptor (Fas) on activated immune cells, which results in cell apoptosis. To determine whether FasL has a role in maternal immune tolerance to the fetus, we looked for the expression of FasL in the human placenta. Immunoperoxidase staining localized FasL to both syncytiotrophoblast and cytotrophoblast in placental villi and chorionic extravillous trophoblast. Western analysis demonstrated FasL in placental villi and a human first-trimester trophoblast cell line (ED27). In contrast, Fas was colocalized to CD45 (leukocyte common antigen) positive cells found in maternal decidua. When isolated peripheral blood lymphocytes were induced to express Fas with phytohemagglutinin (PHA) and interleukin-2 (IL-2) and then cocultured with trophoblast, 30% of the lymphocytes underwent apoptosis, as determined by the in situ death (TUNEL) assay. Neutralizing antibodies to FasL inhibited apoptosis by 40% in these studies. In contrast, activated lymphocytes cocultured with non-FasL-expressing fibroblasts or unactivated non-Fas-expressing lymphocytes cocultured with ED27 trophoblast showed little evidence of apoptosis. These findings suggest that FasL expressed by fetal trophoblast cells can induce apoptosis in activated lymphocytes there by providing a mechanism for maternal immune tolerance to the fetus.
authors.affiliation: Department of Obstetrics/Gynecology, Virginia Commonwealth University/Medical College of Virginia, Richmond 23298, USA. skauma@hsc.vcu.edu
authors.forename: S W
authors.initials: SW
authors.lastname: Kauma
authors.forename: T F
authors.initials: TF
authors.lastname: Huff
authors.forename: N
authors.initials: N
authors.lastname: Hayes
authors.forename: A
authors.initials: A
authors.lastname: Nilkaeo
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002460:Cell Line; D053222:Fas Ligand Protein; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005333:Fetus / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D017493:Leukocyte Common Antigens / Q000276:immunology; D008024:Ligands; D008214:Lymphocytes / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D010920:Placenta / Q000276:immunology / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation; D019014:fas Receptor / Q000276:immunology
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008024:Ligands; D008562:Membrane Glycoproteins; D019014:fas Receptor; D017493:Leukocyte Common Antigens