Molecular cloning and characterization of a locus responsible for O acetylation of the O polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide.
Complementation experiments, Tn5 mutagenesis, and DNA sequencing were used to identify a locus (lag-1) that participates in acetylation of Legionella pneumophila serogroup 1 lipopolysaccharide. Nuclear magnetic resonance analyses of lipopolysaccharides from mutant and complemented strains suggest that lag-1 is responsible for O acetylation of serogroup 1 O polysaccharide.
Zou
C H
CH
Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida, 33101, USA.
Knirel
Y A
YA
Helbig
J H
JH
Zähringer
U
U
Mintz
C S
CS
D000107:Acetylation; D000123:Acetyltransferases; D001426:Bacterial Proteins; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000096:biosynthesis* / Q000737:chemistry; D010455:Peptides / Q000235:genetics*; D011134:Polysaccharides / Q000378:metabolism; D017421:Sequence Analysis; D012703:Serotyping
D001426:Bacterial Proteins; D019081:O Antigens; D010455:Peptides; D011134:Polysaccharides; C120065:lag-1 protein, Legionella; D000123:Acetyltransferases