Characterization of an alternatively spliced isoform of rat vesicle associated membrane protein-2 (VAMP-2).
VAMPs are vesicle associated membrane proteins that are essential for secretion. A spliced isoform of rat VAMP-2, called VAMP-2B, is characterized in this study. The VAMP-2B transcript is the result of alternative RNA splicing in which an intron is retained. The predicted amino acid sequence of VAMP-2B differs from VAMP-2 at its carboxy-terminal end. Because recent studies have shown that VAMP's carboxy-terminal end influences the protein's sorting, the location of myc-epitope tagged VAMP-2B in PC12 cells was determined. Subcellular fractionation showed colocalization of myc-VAMP-2B and endogenous VAMP-2. Thus alternative RNA splicing does not affect VAMP-2 sorting in PC12 cells.
Mandic
R
R
Department of Medicine and the Digestive Disease Center, Stanford University, CA 94305-5487, USA.
Lowe
A W
AW
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016716:PC12 Cells; D050683:R-SNARE Proteins; D051381:Rats; D014018:Tissue Distribution
D008565:Membrane Proteins; D050683:R-SNARE Proteins