Assessment of induced rat mammary tumour response to chemotherapy using the apparent diffusion coefficient of tissue water as determined by diffusion-weighted 1H-NMR spectroscopy in vivo.
Chemosensitivity of N-methyl-N-nitrosourea-induced rat mammary tumours treated with 5-fluorouracil at a dose of 100 mg kg(-1) i.p. was assessed by using diffusion-weighted 1H-MRS to measure the average diffusion coefficient (ADC) of water in the tumour tissue. ADC measurements prior to any therapy correlated positively with necrotic fraction. Tumours with low initial ADC (< 0.95 x 10(9) m2 s(-1)) showed an increase in ADC 7 days after treatment, whereas tumours with a high initial ADC (> 1.2 x 10(9) m2 s(-1)) showed a decrease. All tumours decreased significantly in volume (P < 0.05) 2, 5 and 7 days after treatment. At day 7 post-treatment, tumours with a high pre-treatment ADC started to regrow. The initial ADC value, as well as changes after treatment predict tumour chemosensitivity, which could be clinically relevant.
Lemaire
L
L
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London, UK. laurent.lemaire@univ-angers.fr
Howe
F A
FA
Rodrigues
L M
LM
Griffiths
J R
JR
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D002273:Carcinogens / Q000633:toxicity; D004058:Diffusion; D005069:Evaluation Studies as Topic; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D008770:Methylnitrosourea / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D014867:Water / Q000378:metabolism*
D000964:Antimetabolites, Antineoplastic; D002273:Carcinogens; D014867:Water; D008770:Methylnitrosourea; D005472:Fluorouracil