title: Ultrastructural localization of anionic sites and lectin-binding sites in sarcoid human alveolar macrophages during interaction with T-lymphocytes.
abstract: Sarcoidosis alveolitis is caused by an unknown stimulus activating alveolar macrophages (AM) and T-lymphocytes. During antigen presentation, the complex HLA class II molecule/processed peptide, on the surface of sarcoid AM, induces the T-lymphocyte to proliferate. Altered glycosylation patterns of cell surface glycoproteins such as class II molecules in inflammatory states, may enhance the antigen-presenting capability of AM. In order to know if anionic sites and lectin-binding sites take part in the process of antigen presentation by alveolar macrophages, cells obtained from bronchoalveolar lavage of patients with pulmonary sarcoidosis were incubated with cationized ferritin (CF) and colloidal gold complexed lectins (BSL-I-A4; RCA-I; RCA-II; WGA) for 30 min at 4 degrees C. After incubation, the cells were fixed with 4% paraformaldehyde, 2% glutaraldehyde, postfixed, and Epon embedded. The CF particles were uniformly distributed over the entire cell surface of the lymphocyte, and formed clusters on the surface of the macrophage mainly at the adhesion region between the AM and the lymphocytes. We found enhanced binding of BSL-I-A4 by AM, while WGA and RCA were poorly taken up by these cells. Gold-BSL-I-A4 was distributed randomly on the plasma membrane of the AM, and clustered in the adhesion region with lymphocytes. These results suggest that anionic sites and alpha-D-N-acetyl-galactosamine residues labeled with gold-BSL-I-A4 may be involved in the process of antigen presentation by sarcoid alveolar macrophages.
authors.affiliation: Department of Histology and Embryology, State University of Rio de Janeiro, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Thole
authors.forename: A C
authors.initials: AC
authors.lastname: Stumbo
authors.forename: C H
authors.initials: CH
authors.lastname: Costa
authors.forename: G
authors.initials: G
authors.lastname: Milward
authors.forename: L C
authors.initials: LC
authors.lastname: Porto
authors.forename: L
authors.initials: L
authors.lastname: Carvalho
mesh_terms: D000838:Anions / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis*; D002450:Cell Communication; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D007365:Intercellular Junctions / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*; D037102:Lectins; D008213:Lymphocyte Activation; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D017565:Sarcoidosis, Pulmonary / Q000276:immunology / Q000473:pathology; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure
chemical_list: D000838:Anions; D002241:Carbohydrates; D006681:HLA-D Antigens; D037102:Lectins