title: Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment.
abstract: The objective of this study was to evaluate the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) concentration on regeneration of alveolar bone and cementum, and on associated root resorption and ankylosis. Contralateral, critical size, supra-alveolar, periodontal defects were surgically produced and immediately implanted with rhBMP-2 in an absorbable collagen sponge (ACS) carrier in 8, young adult, male, beagle dogs. 6 animals received rhBMP-2/ACS (rhBMP-2 at 0.05, 0.10, or 0.20 mg/mL; total construct volume/defect approximately 4.0 mL) in contralateral defects following an incomplete block design. 2 animals received rhBMP-2/ACS (rhBMP-2 at 0 and 0.10 mg/mL) in contralateral defects (controls). The animals were euthanised at 8 weeks post-surgery and block sections of the defects were collected for histologic and histometric analysis. Supra-alveolar periodontal defects receiving rhBMP-2 at 0.05, 0.10, or 0.20 mg/ml exhibited extensive alveolar regeneration comprising 86%, 96%, and 88% of the defect height, respectively. Cementum regeneration encompassed 8%, 6%, and 8% of the defect height, respectively. Root resorption was observed for all rhBMP-2 concentrations. Ankylosis was observed in almost all teeth receiving rhBMP-2. Control defects without rhBMP-2 exhibited limited, if any, evidence of alveolar bone and cementum regeneration, root resorption, or ankylosis. Within the selected rhBMP-2 concentration and observation interval, there appear to be no meaningful differences in regeneration of alveolar bone and cementum. There also appear to be no significant differences in the incidence and extent of root resorption and ankylosis, though there may be a positive correlation with rhBMP-2 concentration.
authors.affiliation: Bone Biology and Applications, Genetics Institute, Inc., Andover, MA, USA. uwikesjo@dental.temple.edu
authors.forename: U M
authors.initials: UM
authors.lastname: Wikesjö
authors.forename: P
authors.initials: P
authors.lastname: Guglielmoni
authors.forename: A
authors.initials: A
authors.lastname: Promsudthi
authors.forename: K S
authors.initials: KS
authors.lastname: Cho
authors.forename: L
authors.initials: L
authors.lastname: Trombelli
authors.forename: K A
authors.initials: KA
authors.lastname: Selvig
authors.forename: L
authors.initials: L
authors.lastname: Jin
authors.forename: J M
authors.initials: JM
authors.lastname: Wozney
mesh_terms: D020341:Absorbable Implants; D016301:Alveolar Bone Loss / Q000188:drug therapy* / Q000601:surgery; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000009:adverse effects / Q000494:pharmacology* / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D003094:Collagen; D003739:Dental Cementum / Q000187:drug effects*; D003758:Dental Implantation, Endosseous / Q000379:methods; D015921:Dental Implants; D004285:Dogs; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D008334:Mandible; D017622:Periodontal Attachment Loss / Q000188:drug therapy* / Q000601:surgery; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D012391:Root Resorption / Q000209:etiology; D020254:Tooth Ankylosis / Q000209:etiology; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D015921:Dental Implants; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen