Dietary supplementation of selenomethionine reduces metastasis of melanoma cells in mice.
The present study investigated the effect of dietary supplementation of selenomethionine on pulmonary metastasis of B16BL6 murine melanoma cells in C57BL/6 mice. Mice were assigned to four groups of 15 each. They were fed a basal AIN93G diet and the basal diet supplemented with 2.5 ppm or 5 ppm selenium as selenomethionine or with 2.5 ppm selenium as selenite for two weeks before and after the intravenous injection of 0.5 x 10(5) tumor cells. At necropsy, the number and size of tumors that developed in the lungs were determined. The number of mice that had > or = 11 tumors was 13, 8, 8, and 6 (p < 0.02 compared with the control), and the median number of lung tumors was 64, 14, 12 (p < 0.05 compared with the control), and 8 (p < 0.01 compared with the control) in the control group and the groups with 2.5 ppm and 5 ppm selenium as selenomethionine and 2.5 ppm selenium as selenite. Dietary supplementation of selenomethionine decreased tumor cross-sectional area and tumor volume compared with the controls. At the same dietary level, selenite had a greater inhibitory effect on tumor size than selenomethionine. These results demonstrate that dietary supplementation of selenomethionine reduced experimental metastasis of melanoma cells in mice and inhibited the growth of metastatic tumors that formed in the lungs. It is concluded that selenomethionine is an active form of selenium that reduces experimental metastasis.
Yan
L
L
Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA. linyan@creighton.edu
Yee
J A
JA
Li
D
D
McGuire
M H
MH
Graef
G L
GL
D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage*; D019587:Dietary Supplements; D008099:Liver / Q000378:metabolism; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary*; D008297:Male; D008546:Melanoma, Experimental / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D012643:Selenium / Q000493:pharmacokinetics; D012645:Selenomethionine / Q000008:administration & dosage*
D016588:Anticarcinogenic Agents; D012645:Selenomethionine; D012643:Selenium