Purification and characterisation of a minor low-sulphated dermatan sulphate-proteoglycan from ray skin.
A minor low-sulphated dermatan sulphate proteoglycan was isolated from ray skin by extraction with 2% sodium dodecyl sulphate, followed with ion-exchange chromatography, gel chromatography and density gradient centrifugation. The proteoglycan with a relative molecular mass (Mr) ranging from 70 to 120 kDa is composed of about two dermatan sulphate chains (Mr 33 kDa) bound on a protein core of Mr 27 kDa, and oligosaccharides consisting of uronic acids, hexosamines and neutral sugars. The major amino acids of the protein core were glycine (corresponding to about one-fourth of the total amino acids), serine, threonine, glutamic acid/glutamine, leucine and cysteine, together amounting to 56% of the total. The isolated proteoglycan does not interact with hyaluronic acid and does not form self-aggregates. Dermatan sulphate was rich in iduronic acid (62% of total uronic acid) and composed of non-sulphated (44%), and mono-sulphated disaccharides bearing esterified sulphate groups at positions C-4 (53%) or C-6 (3%) of the N-acetyl galactosamine. HPLC analysis of a pure preparation of dermatan sulphate, showed the presence of galactose and glucose possibly as branches on the dermatan sulphate chain.
Chatziioannidis
C C
CC
Department of Chemistry, University of Patras, Greece.
Karamanos
N K
NK
Anagnostides
S T
ST
Tsegenidis
T
T
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000737:chemistry / Q000302:isolation & purification*; D004586:Electrophoresis; D012860:Skates, Fish; D012867:Skin / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry
D000596:Amino Acids; D011508:Chondroitin Sulfate Proteoglycans; D013464:Sulfuric Acids; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate