title: Regional changes in the expression of neurotrophic factors and their receptors following acute traumatic brain injury in the adult rat brain.
abstract: We have analyzed the effect of severe traumatic brain injury (TBI) on the levels of mRNA expression of neurotrophic factors (NTFs): brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), ciliary neurotrophic factor (CNTF) and their respective receptors: trkB, trkA and CNTFRalpha. The expression was examined in the region of the lesion as well as a region remote from the lesion at 12, 24, and 36 h following the injury. Our data suggest that after the brain injury, the expression of NGF and BDNF mRNAs were early, transiently and significantly upregulated while that of CNTF was a slow and less amplified response in both areas of the brain. We also found that trkA mRNA expression was only upregulated significantly in the remote area; trkB mRNA showed no significant change in either area except an upregulation at 12 h in the remote area. CNTFRalpha was downregulated significantly by 24-36 h in the lesion area and by 24 h in the remote area. These changes suggest that TBI regulates the expression of NTFs and their receptors. These alterations in expression may be involved in modulating the neuronal response after brain injury.
authors.affiliation: Department of Neurosurgery, Emory University School of Medicine, Atlanta, GA 30322, USA. noyesik@emory.edu
authors.forename: N M
authors.initials: NM
authors.lastname: Oyesiku
authors.forename: C O
authors.initials: CO
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: Houston
authors.forename: R S
authors.initials: RS
authors.lastname: Darrell
authors.forename: J S
authors.initials: JS
authors.lastname: Smith
authors.forename: Z L
authors.initials: ZL
authors.lastname: Fulop
authors.forename: C E
authors.initials: CE
authors.lastname: Dixon
authors.forename: D G
authors.initials: DG
authors.lastname: Stein
mesh_terms: D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000235:genetics; D001930:Brain Injuries / Q000503:physiopathology*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D020934:Ciliary Neurotrophic Factor; D017931:DNA Primers; D015870:Gene Expression / Q000502:physiology; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics; D009473:Neuronal Plasticity / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020801:Receptor, Ciliary Neurotrophic Factor; D020917:Receptor, trkA; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor; D017931:DNA Primers; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA