title: Evidence for the presence of major peripheral myelin glycoprotein P0 in mammalian spinal cord and a change of its glycosylation state during aging.
abstract: Glycoproteins, which react with Lens culinaris agglutinin, in the membrane preparation of various portions of brains and spinal cords, obtained from 9-week-old rats and 29-month-old rats, were comparatively analyzed by SDS-polyacrylamide gel electrophoresis. In contrast to the samples from brain, which showed similar staining patterns in the two different age groups, the glycoprotein patterns of spinal cords showed marked differences by the age of donors. The most prominent evidence is that a glycoprotein with an apparent molecular weight of 30 kDa (gp30) was detected in the aged rats, but not in the young adult rats. Based on the amino acid sequence data around the glycosylation site, the gp30 was identified as P0, which is a member of immunoglobulin superfamily and a major structural component of mammalian peripheral nerve myelin. This is the first report indicating that P0, which has been considered as a peripheral nerve-specific glycoprotein, occurs also in the spinal cord of mammals. In addition, nonglycosylated P0 molecule could be detected in the spinal cord of young adult rats by anti-P0 polyclonal antibody. These results indicate that the glycosylation state of the P0 molecule in the spinal cord changes during aging.
authors.affiliation: Department of Glycobiology and Director's Office, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: C
authors.initials: C
authors.lastname: Yasuda
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: A
authors.initials: A
authors.lastname: Kobata
authors.forename: T
authors.initials: T
authors.lastname: Endo
mesh_terms: D000375:Aging / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D037102:Lectins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018993:Myelin P0 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D037121:Plant Lectins; D051381:Rats; D011916:Rats, Inbred F344; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D037102:Lectins; D018993:Myelin P0 Protein; D037121:Plant Lectins; C033619:lentil lectin