An immunoaffinity column for the determination of peanut protein in chocolate.
An immunoaffinity column was prepared from rabbit polyclonal antiserum for the determination of peanut protein from food matrixes. The anti-peanut immunoglobulin G was isolated from antiserum by affinity chromatography on a column coupled with peanut protein and then attached to an AminoLink gel. The column was applied to the determination of peanut protein in chocolate after extraction, immunoaffinity chromatography, and enzyme-linked immunosorbent assay (ELISA). Overall recoveries from chocolate spiked with 0.2-3.2 micrograms/g of peanut protein averaged 77% (range, 72-84%), and the minimum detection limit was 0.1 microgram/g. Chromatography of extracts with the column improved detection limit and eliminated the matrix effect experienced with direct ELISA of chocolate extracts.
Newsome
W H
WH
Health Canada, Health Protection Branch, Bureau of Chemical Safety, Food Research Division, Ottawa, ON, Canada.
Abbott
M
M
D000818:Animals; D010367:Arachis / Q000276:immunology; D002099:Cacao / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D015203:Reproducibility of Results
D007074:Immunoglobulin G; D010940:Plant Proteins