Application of the polymerase chain reaction to detect fowl adenoviruses.
The possibility of using the polymerase chain reaction (PCR) for the detection of fowl adenoviruses (FAdV) was tested. The optimal reaction parameters were evaluated and defined for purified genomic DNA of type 8 fowl adenovirus (FAdV-8), and then the same conditions were applied for nucleic acid extracted from infected cells. One hundred picograms of purified viral DNA, or 250 FAdV-8-infected cells, were detected by ethidium bromide staining of the PCR products in agarose gels. The sensitivity was increased to 10 pg purified viral DNA, or 25 infected cells, when the PCR products were hybridized with a specific labeled probe. Several field isolates of FAdV and the CELO virus (FAdV serotype 1) could be amplified by the same primers and conditions, but the size of the amplicons was smaller than that for the FAdV-8 PCR product. Other avian viruses and uninfected cell cultures tested negative.
Jiang
P
P
Department of Pathobiology, Ontario Veterinary College, University of Guelph.
Ojkic
D
D
Tuboly
T
T
Huber
P
P
Nagy
E
E
D000257:Adenoviridae Infections / Q000175:diagnosis / Q000662:veterinary; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics / Q000302:isolation & purification*; D006528:Carcinoma, Hepatocellular; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008113:Liver Neoplasms; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D004279:DNA, Viral