title: Transcription regulation by repressosome and by RNA polymerase contact.
abstract: The original model of repression of transcription initiation is steric interference of RNA polymerase binding to a promoter by its repressor protein bound to a DNA site that overlaps the promoter. From the results described here, we propose two other mechanisms of repressor action, both of which involve formation of higher-order DNA-multiprotein complexes. These models also explain the problem of RNA polymerase gaining access to a promoter in the condensed nucleoid in response to an inducing signal to initiate transcription.
authors.affiliation: Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255, USA.
authors.forename: S
authors.initials: S
authors.lastname: Adhya
authors.forename: M
authors.initials: M
authors.lastname: Geanacopoulos
authors.forename: D E
authors.initials: DE
authors.lastname: Lewis
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: T
authors.initials: T
authors.lastname: Aki
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D012097:Repressor Proteins; D012321:DNA-Directed RNA Polymerases