title: The subgenus-specific C-terminal region of protein IX is located on the surface of the adenovirus capsid.
abstract: We have investigated the antigenicity of the C- and N-terminal halves of pIX of human adenovirus types 2 and 3 (Ad2 and Ad3) as well as their orientations in virions. We found that only the C-terminal halves of Ad2 pIX and Ad3 pIX reacted in a subgenus-specific manner by enzyme-linked immunosorbent assay and immunoblot analysis. Based on immunoelectron microscopy experiments, pIX in viral capsids appears to be positioned such that the C-terminal part of pIX constitutes the surface domain whereas the N terminus of the protein makes up the internal domain in icosahedral Ad capsids.
authors.affiliation: Institute of Medical Microbiology, Ernst-Moritz-Arndt-University, D-17487 Greifswald, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Akalu
authors.forename: H
authors.initials: H
authors.lastname: Liebermann
authors.forename: U
authors.initials: U
authors.lastname: Bauer
authors.forename: H
authors.initials: H
authors.lastname: Granzow
authors.forename: W
authors.initials: W
authors.lastname: Seidel
mesh_terms: D000260:Adenoviruses, Human / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D001665:Binding Sites; D002213:Capsid / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D036022:Capsid Proteins; D015870:Gene Expression; D006801:Humans; D009500:Neutralization Tests; D010957:Plasmids; D011817:Rabbits; D014771:Virion
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus