Effects of surfactants on the activity of phospholipase D.
We studied the dependence of the activity of cabbage phospholipase A on the substrate (phosphatidylcholine) the aggregated state of which is regulated by addition of either anionic (sodium dodecyl sulfate, cholate or oleate) or cationic (cetyl-trimethylammonium bromide) surfactants. Activation of the enzyme induced by anionic surfactants was shown to correlate with the size of their polar groups. The phospholipase hydrolase activity correlated with the transformation of multilayer liposomes into micelles. The relationship between the processes was of a complex character. The dependence of the amount of enzymically released choline on the calcium concentration passed through a sharp maximum in the presence of the anionic detergents and monotonically increased in the presence of the cationic detergent. In the former case, the sharp increase in the enzyme activity was suggested to be caused by precipitation of phospholipase D with the anionic detergent calcium salt, which can be considered as a specific type of immobilization.
Shumilina
E V
EV
Institute of Chemistry, Far Eastern Division of the Russian Academy of Sciences, Vladivostok.
Zonova
N Y
NY
Shchipunov
Y A
YA
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002642:Chick Embryo; D020355:Cholates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019301:Oleic Acid / Q000494:pharmacology; D010739:Phospholipase D / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D002593:Cetrimonium Compounds; D020355:Cholates; D013501:Surface-Active Agents; D019301:Oleic Acid; D012967:Sodium Dodecyl Sulfate; D010739:Phospholipase D; D002122:Calcium Chloride; D000077286:Cetrimonium