Improved latex agglutination test for detection of antibodies in serum and cerebrospinal fluid of Trypanosoma brucei gambiense infected patients.
A rapid latex agglutination test (LATEX/T. b. gambiense) for detection of antibodies in patients infected with Trypanosoma brucei gambiense is presented. The reagent is coated with a mixture of three variable surface antigens of bloodstream form trypanosomes. Two hundred and forty sera and 79 CSF samples from patients with parasitologically confirmed trypanosome infection along with 173 sera and 38 CSF samples from non-trypanosomiasis patients have been tested. At 1:16 serum dilution, test specificity was 99%, while sensitivity ranged from 83.8 to 100% depending on the geographical origin of the samples. Undiluted CSF samples from non-trypanosomiasis and from first stage patients scored negative while 42 out of 66 CSF samples from second stage patients were positive. Stability and reproducibility of the lyophilized reagent were excellent.
Büscher
P
P
Department of Parasitology, University of Antwerp, Institute of Tropical Medicine, Belgium. pbuscher@itg.be
Lejon
V
V
Magnus
E
E
Van Meirvenne
N
N
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000134:cerebrospinal fluid*; D000953:Antigens, Protozoan / Q000276:immunology; D006801:Humans; D007841:Latex Fixation Tests; D012698:Serologic Tests; D014347:Trypanosoma brucei gambiense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000175:diagnosis*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D014643:Variant Surface Glycoproteins, Trypanosoma