title: Regulation of transcription by a protein methyltransferase.
abstract: The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors, but only when GRIP1 or SRC-1a was coexpressed. Thus, CARM1 functions as a secondary coactivator through its association with p160 coactivators. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduced both methyltransferase and coactivator activities. Thus, coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.
authors.affiliation: Department of Pathology HMR 301, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA.
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Hong
authors.forename: S S
authors.initials: SS
authors.lastname: Koh
authors.forename: S M
authors.initials: SM
authors.lastname: Huang
authors.forename: B T
authors.initials: BT
authors.lastname: Schurter
authors.forename: D W
authors.initials: DW
authors.lastname: Aswad
authors.forename: M R
authors.initials: MR
authors.lastname: Stallcup
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D056920:Nuclear Receptor Coactivator 1; D051958:Nuclear Receptor Coactivator 2; D056921:Nuclear Receptor Coactivator 3; D011484:Protein-Arginine N-Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D006657:Histones; C498002:Ncoa2 protein, mouse; D051958:Nuclear Receptor Coactivator 2; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011988:Receptors, Thyroid Hormone; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D011484:Protein-Arginine N-Methyltransferases; C119966:coactivator-associated arginine methyltransferase 1; D051548:Histone Acetyltransferases; C538861:Ncoa1 protein, mouse; C538867:Ncoa3 protein, mouse; D056920:Nuclear Receptor Coactivator 1; D056921:Nuclear Receptor Coactivator 3