title: Phenotype and cytokine profile of Schistosoma mansoni specific T cell lines and clones derived from schistosomiasis patients with distinct clinical forms.
abstract: It is essential to distinguish the role of T lymphocytes on the physiopathology associated to more severe forms of schistosomiasis and on the immunomodulation that evolves in the majority of infected people. In this study, we generated Schistosoma mansoni-specific T cell lines and clones from patients with the acute and chronic (intestinal and hepatosplenic forms) phases of disease, from former ones, and from uninfected individuals sensitized to parasite soluble antigens. T cell lines derived from nontreated acute infected donors were capable of producing IL-4 and IL-5, while cells from treated patients secreted IFN-gamma. Lines from intestinal chronic and antigen-sensitized donors preferentially produced IFN-gamma, while those from hepatosplenic patients secreted all three cytokines. The cytokine analysis of CD4+ T cell clones revealed a Th2/Th0 pattern (clones producing IL-4 and IL-5 and clones producing all three cytokines) for those derived from infected patients, while cells from antigen-sensitized donors exhibited an opposite Th1/Th0 pattern (clones producing IFN-gamma and clones producing all three cytokines). The possible role of these T cell populations on human schistosomiasis mansoni is discussed.
authors.affiliation: Departamento Bioquímica e Imunologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Contigli
authors.forename: D N
authors.initials: DN
authors.lastname: Silva-Teixeira
authors.forename: G
authors.initials: G
authors.lastname: Del Prete
authors.forename: M M
authors.initials: MM
authors.lastname: D'Elios
authors.forename: M
authors.initials: M
authors.lastname: De Carli
authors.forename: M
authors.initials: M
authors.lastname: Manghetti
authors.forename: A
authors.initials: A
authors.lastname: Amedei
authors.forename: F
authors.initials: F
authors.lastname: Almerigogna
authors.forename: J R
authors.initials: JR
authors.lastname: Lambertucci
authors.forename: A M
authors.initials: AM
authors.lastname: Goes
mesh_terms: D000208:Acute Disease; D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage; D002460:Cell Line; D002908:Chronic Disease; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis*; D006099:Granuloma / Q000276:immunology; D006801:Humans; D007114:Immunization; D008213:Lymphocyte Activation; D010641:Phenotype; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D016207:Cytokines