title: Establishment and characterization of a novel ALL-L3 cell line (BALM-18): induction of apoptosis by anti-IgM and inhibition of apoptosis by bone marrow stroma cells.
abstract: A human acute lymphoblastic leukemia (ALL) cell line, BALM-18, was established from the peripheral blood specimen of a patient with B cell ALL L3 type (ALL-L3) at diagnosis using bone marrow stroma cells (BST) as feeder cells. The primary leukemia cells did not grow without feeder cells. As with the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10+, CD20+, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL-L3 and Burkitt's lymphoma. It also revealed a significantly low level of bcl-2 protein. Strikingly, anti-human IgM antibody did induce apoptosis in induction experiments. However, it was reversed by the addition of anti-CD40 antibody or BST cells, whereas the culture supernatant of the stroma cells did not show any effect on the inhibition of apoptosis. BALM-18 may be useful for analyzing both the mechanisms of anti-IgM induced apoptosis and signaling during the inhibition of apoptosis by CD40 or BST cells.
authors.affiliation: Fujisaki Cell Center, Hayashibara Biochemical Labs. Inc., Okayama, Japan. yomatsuo@hayashibara.co.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuo
authors.forename: A
authors.initials: A
authors.lastname: Sugimoto
authors.forename: A
authors.initials: A
authors.lastname: Harashima
authors.forename: C
authors.initials: C
authors.lastname: Nishizaki
authors.forename: F
authors.initials: F
authors.lastname: Ishimaru
authors.forename: E
authors.initials: E
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
authors.forename: M
authors.initials: M
authors.lastname: Harada
authors.forename: K
authors.initials: K
authors.lastname: Orita
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000502:physiology*; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D001854:Bone Marrow Cells; D002051:Burkitt Lymphoma / Q000473:pathology*; D019013:CD40 Antigens / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D016130:Immunophenotyping; D007621:Karyotyping; D008297:Male; D017154:Stromal Cells; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019013:CD40 Antigens; D007075:Immunoglobulin M