Molecular cloning and sequence analysis of the cDNA encoding beta-lactoglobulin in Bubalus bubalis.
The cDNA for bubaline beta-lactoglobulin (beta lg) has been cloned through RT-PCR approach and sequenced. Sequence data showed a single open reading frame coding for a protein of 180 amino acids with a signal sequence of 18 amino acid residues. Comparison with other ruminant beta lg sequences revealed a high homology indicating the protein to be conserved through evolution. The degree of homology, at amino acid level, is 96.1%, 95.6%, 93.9% and 63.7% with goat, sheep, cow and pig, respectively.
Das
P
P
Gene Regulation Laboratory, National Institute of Immunology, New Delhi, India.
Jain
S
S
Nayak
S
S
Apparao
K B
KB
Totey
S M
SM
Garg
L C
LC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007782:Lactoglobulins / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D007782:Lactoglobulins