A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris.
Zeatin is the most active and ubiquitous form of the naturally occurring cytokinins. Glycosyl conjugates of zeatin are found in many plant tissues and are considered important for storage and protection against degradative enzymes. Two enzymes catalyzing the formation of O-glycosyl derivatives of zeatin have been characterized, O-glucosyltransferase and O-xylosyltransferase, occurring in seeds of lima bean (Phaseolus lunatus) and bean (Phaseolus vulgaris), respectively. Recently, the ZOG1 gene (zeatin O-glucosyltansferase) was isolated from P. lunatis (). Based on the ZOG1 sequence, the ZOX1 gene (zeatin O-xylosyltransferase) was cloned from P. vulgaris. ZOX1 contains an open reading frame of 1362 bp that codes for a 454-amino acid peptide of 51 kD. The recombinant protein has properties identical to the native enzyme: it catalyzes O-xylosylzeatin formation with UDP-Xyl as a glycosyl donor but does not recognize UDP-Glucose as a substrate. The ZOX1 and ZOG1 genes exhibit 93% identity at the nucleotide level and 90% similarity at the amino acid level. Neither gene contains introns. These zeatin-specific genes and their promoters will be useful for studies of the regulation of active versus storage forms of cytokinins. Comparison of sequences encoding similar enzymes with distinct substrate specificity may lead to identification of epitopes specific to cytokinin and glycosyl donor molecules.
Martin
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RC
Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon 97331-7304, USA.
Mok
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Mok
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D001483:Base Sequence; D003001:Cloning, Molecular; D003583:Cytokinins / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000235:genetics; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015026:Zeatin / Q000378:metabolism
D003583:Cytokinins; D017931:DNA Primers; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins; D015026:Zeatin; D005964:Glucosyltransferases; C117387:UDPglucose zeatin O-glucosyltransferase, plant; D010430:Pentosyltransferases; C120478:zeatin O-beta-D-xylosyltransferase protein, Phaseolus vulgaris