Enhancement of activity-dependent calcium increase by neurotrophin-4 in visual cortex pyramidal neurons.
In pyramidal neurons from rat visual cortex slices, bath-application of NT-4 (20 ng/ml) did not much affected the baseline calcium signal, but did enhance calcium signals elicited by injections of depolarizing currents (+0.5 nA, 1 s). This enhancing effect of NT-4 was abolished by co-applying K252a. With ryanodine injected intracellularly, the effect of NT-4 was significantly reduced, suggesting an involvement of intracellular calcium release in this NT-4-induced enhancement of calcium transient.
Kato
N
N
Department of Integrative Brain Science, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan. f50207@sakura.kudpc.kyoto-u.ac.jp
Tanaka
T
T
Yamamoto
K
K
Isomura
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D014793:Visual Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; C073922:neurotrophin 4; D002118:Calcium