title: Lectin-binding properties of different Leishmania species.
abstract: Carbohydrate cell-surface residues on stationary promastigotes of 19 isolates of Leishmania were studied with a panel of 27 highly purified lectins, which were specific for N-acetyl-D-glucosamine, D-mannose, L-fucose, D-galactose, N-acetyl-D-galactosamine, and sialic acid. The specificity of the cell-surface carbohydrates was analyzed by agglutination and radioiodinated lectin-binding assays. L. (L.) amazonensis and L. (L.) donovani were agglutinated by 12 and 10 of the 27 lectins used, respectively. Artocarpus integrifolia lectin (Jacalin) was incapable of agglutinating the tested species of the donovani complex, and this result was confirmed by radioiodinated Jacalin-binding assays. Jacalin had an average of 3.8 x 10(6) receptors/L. (L) amazonensis promastigote and bound with an association constant of 5 x 10(6) M(-1).
authors.affiliation: Departamento Imunologia, Instituto de Microbiologia - UFRJ, CCS, Rio de Janeiro, RJ, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Andrade
authors.forename: E M
authors.initials: EM
authors.lastname: Saraiva
mesh_terms: D000371:Agglutination; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D037102:Lectins / Q000378:metabolism*; D007891:Leishmania / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D007893:Leishmania donovani / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D007894:Leishmania mexicana / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D037121:Plant Lectins; D013045:Species Specificity
chemical_list: D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin