Isolation and purification of penicillin G acylase obtained from Escherichia coli (NCIM-2400) and immobilisation on Eupergit C for the production of 6 amino penicillanic acid.
Penicillin G-Acylase is produced by submerged cultivation of E. Coli (NCIM-2400) and extracted from the harvested fermented broth, purified (affinity chromatography) and immobilised on Eupergit C (Synthetic polymer in bead form). The immobilised penicillin G acylase properties are studied and compared with soluble penicillin G-acylase. The control parameters for conversion of penicillin G-K to 6 APA are optimised [e.g. substrate (Pen G-K) concentration ratio to immobilised penicillin G-acylase, temperature, pH etc.] in a stirred tank reactor. Our findings suggest that immobilised penicillin G-acylase can be used commercially and the productivity of 1 kg. of immobilised enzyme is around 400 kg of 6 APA under given desired stipulated conditions.
Hegde
M M
MM
Hindustan Antibiotics Ltd., Pimpri, Pune, India.
Thadani
S B
SB
Singh
U
U
Naik
S R
SR
D004800:Enzymes, Immobilized / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D010399:Penicillin Amidase / Q000302:isolation & purification*; D011108:Polymers
D004800:Enzymes, Immobilized; D011108:Polymers; D010397:Penicillanic Acid; C065843:Eupergit C; D010399:Penicillin Amidase; C004408:aminopenicillanic acid