title: Tomographic reconstruction of the retina using a confocal scanning laser ophthalmoscope.
abstract: Retinal imaging with a confocal scanning laser ophthalmoscope (cSLO) involves scanning a small laser beam over the retina and constructing an image from the reflected light. By applying the confocal principle, tomographic images can be produced. However, the thickness of such slices, when compared with the retinal thickness, is too large to give useful 3D retinal images. In this study an algorithm has been developed which fits a double Gaussian curve to the axial intensity profiles generated from a stack of image slices. The underlying assumption is that the laser light has mainly been reflected by two structures in the retina, the internal limiting membrane and the retinal pigment epithelium. From the fitted curve, topographic images and novel thickness images of the retina can be generated. The technique has been applied to three normal volunteers and seven patients with macular pathology (cystoid macular oedema and macular hole) demonstrating the clinical value of the technique. The improvement in accuracy achieved by using a double rather than a single Gaussian is also demonstrated.
authors.affiliation: Department of Bio-Medical Physics and Bio-Engineering, University of Aberdeen, Foresterhill, UK. p.vieira@biomed.abdn.ac.uk
authors.forename: P
authors.initials: P
authors.lastname: Vieira
authors.forename: A
authors.initials: A
authors.lastname: Manivannan
authors.forename: C S
authors.initials: CS
authors.lastname: Lim
authors.forename: P
authors.initials: P
authors.lastname: Sharp
authors.forename: J V
authors.initials: JV
authors.lastname: Forrester
mesh_terms: D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008268:Macular Degeneration / Q000175:diagnosis*; D009887:Ophthalmoscopy / Q000379:methods*; D019047:Phantoms, Imaging; D012160:Retina / Q000033:anatomy & histology* / Q000502:physiology*; D014054:Tomography / Q000379:methods*