title: Rescue of cyclin D1 deficiency by knockin cyclin E.
abstract: D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene (Ccnd1) have been deleted and replaced by those of human cyclin E (CCNE). In the tissues and cells of these mice, the expression pattern of human cyclin E faithfully reproduces that normally associated with mouse cyclin D1. The replacement of cyclin D1 with cyclin E rescues all phenotypic manifestations of cyclin D1 deficiency and restores normal development in cyclin D1-dependent tissues. Thus, cyclin E can functionally replace cyclin D1. Our analyses suggest that cyclin E is the major downstream target of cyclin D1.
authors.affiliation: Dana-Farber Cancer Institute, and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Geng
authors.forename: W
authors.initials: W
authors.lastname: Whoriskey
authors.forename: M Y
authors.initials: MY
authors.lastname: Park
authors.forename: R T
authors.initials: RT
authors.lastname: Bronson
authors.forename: R H
authors.initials: RH
authors.lastname: Medema
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: R A
authors.initials: RA
authors.lastname: Weinberg
authors.forename: P
authors.initials: P
authors.lastname: Sicinski
mesh_terms: D000818:Animals; D019938:Cyclin D1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011960:Receptors, Estrogen / Q000378:metabolism; D012160:Retina / Q000378:metabolism
chemical_list: D019927:Cyclin E; D011960:Receptors, Estrogen; D019938:Cyclin D1