title: Identification of peptides presented by HLA class I molecules on cervical cancer cells with HPV-18 infection.
abstract: In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85% with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.
authors.affiliation: Laboratorio de Immunobiología, Unidad de Investigación en Diferenciación Celular y Cáncer, Facultad de Estudios Superiores Zaragoza, UNAM, México City, Mexico.
authors.forename: A M
authors.initials: AM
authors.lastname: García
authors.forename: V F
authors.initials: VF
authors.lastname: Ortíz-Navarrete
authors.forename: M L
authors.initials: ML
authors.lastname: Mora-García
authors.forename: F
authors.initials: F
authors.lastname: Flores-Borja
authors.forename: A
authors.initials: A
authors.lastname: Diaz-Quiñonez
authors.forename: A
authors.initials: A
authors.lastname: Isibasi-Araujo
authors.forename: C
authors.initials: C
authors.lastname: Trejo-Becerril
authors.forename: R
authors.initials: R
authors.lastname: Chacón-Salinas
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Montes
authors.forename: J
authors.initials: J
authors.lastname: Granados-Arreola
authors.forename: C
authors.initials: C
authors.lastname: de Leo
authors.forename: B
authors.initials: B
authors.lastname: Weiss-Steider
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000276:immunology*; D030361:Papillomavirus Infections / Q000276:immunology / Q000821:virology; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000276:immunology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D014764:Viral Proteins