title: Apocalmodulin binds to the myosin light chain kinase calmodulin target site.
abstract: The interaction of a 20-residue-long peptide derived from the calmodulin-binding domain of the smooth muscle myosin light chain kinase with calcium-free calmodulin (apocalmodulin) was studied using a combination of isothermal titration calorimetry and differential scanning calorimetry. We showed that: (i) a significant binding between apocalmodulin and the target peptide (RS20) exists in the absence of salt (Ka = 10(6) M-1), (ii) the peptide interacts with the C-terminal lobe of calmodulin and adopts a partly helical conformation, and (iii) the presence of salt weakens the affinity of the peptide for apocalmodulin, emphasizing the importance of electrostatic interactions in the complex. Based on these results and taking into account the work of Bayley et al. (Bayley, P. M., Findlay, W.A., and Martin, S. R. (1996) Protein Sci. 5, 1215-1228), we suggest a physiological role for apocalmodulin.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 117984 Moscow, Russia.
authors.forename: P O
authors.initials: PO
authors.lastname: Tsvetkov
authors.forename: I I
authors.initials: II
authors.lastname: Protasevich
authors.forename: R
authors.initials: R
authors.lastname: Gilli
authors.forename: D
authors.initials: D
authors.lastname: Lafitte
authors.forename: V M
authors.initials: VM
authors.lastname: Lobachov
authors.forename: J
authors.initials: J
authors.lastname: Haiech
authors.forename: C
authors.initials: C
authors.lastname: Briand
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
mesh_terms: D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D002147:Calmodulin; D009219:Myosin-Light-Chain Kinase; D002118:Calcium