title: Serologic and genetic characterization of HIV type 1 subtypes on Reunion Island.
abstract: The aim of this study was to determine the HIV subtypes present on Reunion Island, a French island located in the Indian Ocean, where the first case of AIDS was diagnosed in 1987. Paired sera and blood samples were collected between September 1996 and September 1997 from 53 HIV-1-positive patients. Subtyping was performed by serotyping with a previously described subtype-specific enzyme immunoassay (SSEIA) and by genotyping with the heteroduplex mobility assay (HMA). When samples gave uninterpretable results with either of the methods, or discordant results, the V3 env region was sequenced and genetic subtypes were determined by phylogenetic analysis. Genetic subtyping showed that 48 of 53 patients were infected with HIV-1 subtype B (90.5%). This high prevalence of subtype B on Reunion Island is probably due to the regular exchanges with metropolitan France. The other five patients were infected with subtype A (9.5%); they had been directly linked to African populations. Of the 48 subtype B samples, 44 (91.7%) were correctly subtyped by SSEIA and 43 (89.6%) by HMA. However, the SSEIA did not allow the subtyping of A strains in three of five patients. Thus, the SSEIA could be an alternative routine technique for screening subtype B versus nonsubtype B HIV-1 strains.
authors.affiliation: Department of Virology/RHAP CNRS, Faculty of Medicine/University Hospital, Grenoble, France.
authors.forename: K
authors.initials: K
authors.lastname: Brengel-Pesce
authors.forename: P
authors.initials: P
authors.lastname: Innocenti-Francillard
authors.forename: P
authors.initials: P
authors.lastname: Morand
authors.forename: A
authors.initials: A
authors.lastname: Michault
authors.forename: P
authors.initials: P
authors.lastname: Poubeau
authors.forename: F
authors.initials: F
authors.lastname: Barin
authors.forename: S
authors.initials: S
authors.lastname: Saragosti
authors.forename: J M
authors.initials: JM
authors.lastname: Seigneurin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D005260:Female; D005602:France; D015483:HIV Antibodies / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012703:Serotyping
chemical_list: D004279:DNA, Viral; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments