title: Differentiation of a cell line of human cervical argyrophil small cell carcinoma to macrophage lineage cells.
abstract: To investigate the origin of argyrophil small cell carcinoma (ASCC) of the uterine cervix, we examined the influence of dibutyryl cyclic adenosine 3',5'-monophosphate (dB-cAMP), a known differentiation inducer, on the characteristics of an ASCC cell line, TC-YIK, which has been shown to be a useful in vitro experimental model of ASCC. In TC-YIK cells after treatment with dB-cAMP, two specific antigenic markers of macrophages, CD14 and human leukocyte antigen-DR, were detected by flow cytometric analysis. In addition, interferon-gamma mRNA was detected by reverse transcription-polymerase chain reaction and interferon-gamma protein was detected by ELISA. More than 90% of the cells stained positive for alpha-naphthyl butyrate esterase, 1% of the cells showed phagocytotic activity against Micrococcus lysodeikticus, and 22% of the cells had M. lysodeikticus adsorbed on their surface. Furthermore, granulocyte-macrophage colony stimulating factor accelerated the proliferation of TC-YIK cells. These results indicate that dB-cAMP promotes differentiation of ASCC cells to macrophages. In contrast, less than 10% of the cells showed stellate morphology, suggesting differentiation to neuronal cells after treatment with dB-cAMP, as reported previously. Thus, TC-YIK cells have been shown to differentiate both into macrophage lineage cells and neuronal cells, suggesting that ASCC originates from undifferentiated stem cells.
authors.affiliation: Department of Microbiology, Kyoto Prefectural University of Medicine. ichimura@basic.kpu-m.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Ichimura
authors.forename: N
authors.initials: N
authors.lastname: Sakashita
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Chisaka
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
authors.forename: M
authors.initials: M
authors.lastname: Kita
authors.forename: S
authors.initials: S
authors.lastname: Yuasa
authors.forename: J
authors.initials: J
authors.lastname: Imanishi
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D003994:Bucladesine / Q000494:pharmacology; D018288:Carcinoma, Small Cell / Q000188:drug therapy / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage; D016207:Cytokines / Q000235:genetics; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008264:Macrophages / Q000473:pathology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016622:Silver Staining; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D016207:Cytokines; D012333:RNA, Messenger; D003994:Bucladesine