title: Instrumental biosensors: new perspectives for the analysis of biomolecular interactions.
abstract: The use of instrumental biosensors in basic research to measure biomolecular interactions in real time is increasing exponentially. Applications include protein-protein, protein-peptide, DNA-protein, DNA-DNA, and lipid-protein interactions. Such techniques have been applied to, for example, antibody-antigen, receptor-ligand, signal transduction, and nuclear receptor studies. This review outlines the principles of two of the most commonly used instruments and highlights specific operating parameters that will assist in optimising experimental design, data generation, and analysis.
authors.affiliation: Ludwig Institute for Cancer Research, CRC for Cellular Growth Factors, P.O. Royal Melbourne Hospital, Victoria, Australia. ed.nice@ludwig.edu.au
authors.forename: E C
authors.initials: EC
authors.lastname: Nice
authors.forename: B
authors.initials: B
authors.lastname: Catimel
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D002851:Chromatography, High Pressure Liquid; D018604:Epitope Mapping; D004867:Equipment Design; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008024:Ligands; D020349:Surface Plasmon Resonance / Q000295:instrumentation
chemical_list: D007202:Indicators and Reagents; D008024:Ligands