The role of plasma proteins and stress in the assessment of hemocompatibility.
The physiological and psychological conditions of subjects supplying blood for hemocompatibility tests significantly affect the behavior of platelets in terms of both adhesion and activation. The responses of platelets to a standard biomaterial, polyethylene (PE), were examined with blood collected from male rabbits both in basal conditions and after stress. Different media were utilized. First, platelet-rich plasma (PRP) was used to obtain a PE response to contact with platelets. Then platelets drawn from PRP were isolated and washed with Krebs-Ringer solution. One aliquot was suspended in serum (Pw-S) where fibrinogen was absent, another aliquot in Krebs-Ringer solution (Pw-KR) (in order to avoid the influence of the plasma proteins on platelets), and a third aliquot in the original plasma from which the platelets were drawn (Pw-PPP) (in order to restore the initial condition of the plasma but with washed platelets). The analysis of platelet adhesion and morphology was performed by Scanning Electron Microscopy (SEM). Differences in platelet adhesion and morphology were observed with four different media in nonstressed animals, with Pw-PPP showing a higher number and Pw-S and PW-KR lower numbers. Platelet morphology indicated low levels of activation. The platelets drawn from stressed subjects could not be counted in either PRP or PPP medium because they were fully aggregated and adhered; in contrast, in Pw-KR and Pw-S, no significant differences were found with respect to nonstressed conditions, and there was little difference in platelet morphology. All of these factors underline the role of plasma proteins, in particular fibrinogen, in the stress-induced activation of platelets.
Barbucci
R
R
Department of Chemical and Biosystem Sciences and Technology, University of Siena, Italy.
Lamponi
S
S
Aloisi
A M
AM
D000818:Animals; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D001798:Blood Proteins / Q000502:physiology*; D003345:Corticosterone / Q000097:blood; D003470:Culture Media; D007103:Immobilization; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000502:physiology; D011095:Polyethylenes; D011817:Rabbits; D013315:Stress, Psychological / Q000503:physiopathology*
D001672:Biocompatible Materials; D001798:Blood Proteins; D003470:Culture Media; D011095:Polyethylenes; D003345:Corticosterone