Effect of mitoxantrone on DNA polymerase of Ehrlich ascites carcinoma cells.
OBJECTIVE
To study the effect of mitoxantrone (Mit) on DNA polymerases of tumor cells.


METHODS
DNA polymerases of Ehrlich ascites carcinoma cells were isolated by phosphocellulose column chromatography. The effects of Mit on DNA polymerase alpha, beta, and gamma were detected by method of K Ono.


RESULTS
Mit inhibited DNA polymerase alpha, beta, and gamma, IC50 values were 11.9, 6.5, and 11.9 mumol.L-1, and Ki 1.86, 2.22, and 2.05 mumol.L-1, respectively. The inhibitory mode of Mit on DNA polymerase alpha, beta, and gamma was competitive.


CONCLUSIONS
Mit is a strong inhibitor on DNA polymerase alpha, beta, and gamma. The inhibitory mode was competition with respect to template DNA.
Xian
L J
LJ
Cancer Institute, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
Li
H X
HX
Liu
Z C
ZC
Pan
Q C
QC
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001667:Binding, Competitive; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology* / Q000473:pathology; D004256:DNA Polymerase I / Q000378:metabolism; D019951:DNA Polymerase beta / Q000378:metabolism; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D008942:Mitoxantrone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000201:enzymology
D000970:Antineoplastic Agents; D004317:Doxorubicin; D008942:Mitoxantrone; D004256:DNA Polymerase I; D019951:DNA Polymerase beta; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase