Determination of avilamycin in poultry feeds by liquid chromatography.
Avilamycin was extracted from feed with acetonitrile. Isolation of avilamycin factors from feed matrix interference was accomplished by normal-phase solid-phase extraction with silica as sorbent. Reversed-phase liquid chromatography was subsequently used to separate and quantitate the primary biologically active factors A and B for determination of chemical potency. This method combines specificity for avilamycins A and B in poultry feeds with simple sample preparation that removes matrix interferences. Recoveries of factor A ranged from 93.29 to 97.26%, with precision (relative standard deviation) ranging from 1.1 to 3.4%. Avilamycin factors in feed samples tested ranged from 4.45 to 17.82 micrograms/g for factor A and from 0.80 to 3.18 micrograms/g for factor B.
Scott
C A
CA
A Division of Eli Lilly and Company, Greenfield, IN 46140-0708, USA.
Yordy
D W
DW
Coleman
M R
MR
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; C006653:avilamycin