title: Interleukin-1alpha stimulated prostacyclin release by increasing gene transcription of prostaglandin H synthase and phospholipase A2 in human vascular endothelial cells.
abstract: This study was conducted to evaluate the effects of interleukin-1alpha (IL-1alpha) on prostacyclin (PGI2) production in cultured human vascular endothelial cells in association with intracellular Ca2+, inositol 1,4,5-trisphosphate (IP3), and with prostaglandin H synthase (PGHS) and phospholipase A2 (PLA2) gene expression by using the competitive polymerase chain reaction (PCR) method. IL-1alpha did not increase PGI2 production for 15 min, but induced an increase of about three-fold relative to that in controls at 60 and 180 min. IL- 1alpha had no effect on intracellular Ca2+ levels throughout the experimental period. In this study, consistent with previous reports, PGHS-1 messenger RNA (mRNA) was constitutively expressed, whereas PLA2 mRNA was not. After stimulation with IL-1alpha, PLA2 mRNA level showed an eightfold increase within 15 min, and PGHS-2 mRNA level increased by 76-fold within 180 min. PGHS-1 mRNA level was increased 1.6-fold at 180 min. These results suggest the existence of regulatory mechanisms of IL-1alpha-induced PGI2 production, which involve PGHS and PLA2 gene transcription.
authors.affiliation: Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Toratani
authors.forename: S
authors.initials: S
authors.lastname: Sawada
authors.forename: Y
authors.initials: Y
authors.lastname: Kono
authors.forename: T
authors.initials: T
authors.lastname: Higaki
authors.forename: H
authors.initials: H
authors.lastname: Imamura
authors.forename: Y
authors.initials: Y
authors.lastname: Tada
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Komatsu
authors.forename: N
authors.initials: N
authors.lastname: Akamatsu
authors.forename: T
authors.initials: T
authors.lastname: Tamagaki
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa
authors.forename: H
authors.initials: H
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
mesh_terms: D001483:Base Sequence; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000187:drug effects / Q000235:genetics*; D054467:Phospholipases A2; D016133:Polymerase Chain Reaction; D011451:Prostaglandin-Endoperoxide Synthases / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D015544:Inositol 1,4,5-Trisphosphate; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium