Postmortem proteolysis and calpain/calpastatin activity in callipyge and normal lamb biceps femoris during extended postmortem storage.
The present experiment was conducted to determine whether calpastatin inhibits only the rate, or both the rate and extent, of calpain-induced postmortem proteolysis. Biceps femoris from normal (n = 6) and callipyge (n = 6) lamb was stored for 56 d at 4 degrees C. Calpastatin activity was higher (P < .05) in the callipyge muscle at 0 and 14 d postmortem, but not at 56 d postmortem. The activity of mu-calpain did not differ between normal and callipyge biceps femoris at 0 and 56 d postmortem (P > .05), but was higher at 14 d postmortem in the callipyge muscle (P < 0.05). The activity of m-calpain was higher in the callipyge muscle (P < 0.05). Western blot analyses of titin, nebulin, dystrophin, myosin heavy chain, vinculin, alpha-actinin, desmin, and troponin-T indicated that postmortem proteolysis was less extensive in callipyge than in normal biceps femoris at all postmortem times. The results of this experiment indicate that calpastatin inhibits both the rate and extent of postmortem proteolysis.
Geesink
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USDA, ARS, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933-0166, USA.
Koohmaraie
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M
D000185:Actinin / Q000032:analysis; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002154:Calpain / Q000378:metabolism*; D064211:Connectin; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism*; D003893:Desmin / Q000032:analysis; D016189:Dystrophin / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D009124:Muscle Proteins / Q000032:analysis; D018482:Muscle, Skeletal / Q000201:enzymology*; D018995:Myosin Heavy Chains / Q000032:analysis; D011180:Postmortem Changes; D011494:Protein Kinases / Q000032:analysis; D012756:Sheep / Q000033:anatomy & histology*; D020107:Troponin T / Q000032:analysis; D016596:Vinculin / Q000032:analysis
D002135:Calcium-Binding Proteins; D064211:Connectin; D015853:Cysteine Proteinase Inhibitors; D003893:Desmin; D016189:Dystrophin; D009124:Muscle Proteins; D020107:Troponin T; C035620:nebulin; D000185:Actinin; D016596:Vinculin; C033668:calpastatin; D011494:Protein Kinases; D002154:Calpain; D018995:Myosin Heavy Chains