[Overexpression of p53 protein and its relation to HPV in squamous intraepithelial lesion and cervical carcinoma].
OBJECTIVE
To study the relation between the expression of p53 protein and HPV infection in squamous intraepithelial lesion (SIL) and cervical carcinoma.


METHODS
The expression of p53 protein was determined in the paraffin embedded tissues of 171 cases of cervical carcinoma, 68 cases of SIL and 29 cases of chronic cervicitis, using ABC immunohistochemical method. Detection and typing of HPV were carried out adopting PCR-RFLP technique on the same tissues.


RESULTS
In the cervical carcinomas, the overexpression rate (38.6%) and intensive overexpression rate (11.7%) of p53 protein were higher than those in LSIL (10.0% and 0.0%, respectively) and HSIL (35.4% and 2.1%, respectively). The overexpression rates of p53 protein in SIL and cervical carcinoma with positive HPV 18 were 87.5% and 84.3% respectively, higher than 21.2% and 10.5% in the corresponding groups carrying HPV 16, as well as 16.7% and 57.1% in the corresponding groups negative for HPV.


CONCLUSIONS
The overexpression of p53 protein might play a role in the carcinogenesis of cervical carcinoma. The relation between p53 overexpression and HPV 18 infection in SIL and cervical carcinoma remains to be clarified.
Li
X
X
Department of Gynecology and Obsterics, General Hospital, Tianjin Medical University.
Mi
R
R
Jiao
S
S
D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D014412:Tumor Virus Infections; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000821:virology*; D002575:Uterine Cervicitis / Q000378:metabolism / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000821:virology*
D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53