title: [Dentinogenesis imperfecta. Scanning electron microscopic study and microanalysis].
abstract: BACKGROUND
  Dentinogenesis imperfecta (DI) is an inherited dentine defect which affects both the primary and secondary dentitions. Shields et al. in 1973 suggested a classification of DI within three types: type I, associated with osteogenesis imperfecta (OI), type II and type III. Although the varying clinical, radiographic and histological findings in DI have been described in detail, an available method for closer examination of the abnormal dentine matrix, electron microscopy, has seldom been used. Scanning and transmission electron microscopy studies can help to understand the pathogenesis of the different types of heritable dentine defects and the diagnosis and classification of these diseases. The aim of the present study was to evaluate a case of DI using scanning electron microscopy and microanalysis.
  
  
  METHODS
  Dentine was obtained from tooth samples from a fourteen-year-old boy affected by DI and from third molars extracted for therapeutic reasons used as controls. Samples were observed with a scanning electron microscope, scanning electron micrographs were evaluated with an image analysis program and specimens were finally observed with a scanning electron microscope equipped for micro-analysis.
  
  
  CONCLUSIONS
  The results obtained showed that the total number of dentinal tubules was significantly reduced and the presence of a dentine mineralization defect in the patient affected by DI, in comparison to the controls.
authors.affiliation: Istituto di Scienze Odontostomatologiche, Università degli Studi, Ancona.
authors.forename: E
authors.initials: E
authors.lastname: Salvolini
authors.forename: R
authors.initials: R
authors.lastname: Di Giorgio
authors.forename: E
authors.initials: E
authors.lastname: Caselli
authors.forename: L
authors.initials: L
authors.lastname: De Florio
mesh_terms: D000293:Adolescent; D003627:Data Interpretation, Statistical; D003811:Dentinogenesis Imperfecta / Q000175:diagnosis* / Q000473:pathology; D004577:Electron Probe Microanalysis; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D011862:Radiography, Panoramic
vernacular_title: Dentinogenesi imperfetta. Studio al microscopio elettronico a scansione e microanalisi.