title: Characterisation of a starch-hydrolysing enzyme of Aspergillus niger.
abstract: A UV-induced mutant strain of Aspergillus niger (CFTRI-1105-U9) overproduced a starch-hydrolysing enzyme with properties characteristically different from the known amylases of the fungus. The purified enzyme of 4.0 pI had an apparent molecular mass of 125 kDa and it dextrinised starch and then saccharified the dextrins. Patterns of the enzyme activity on starch, resulting in glucose at 60 degrees C and glucose, maltose and maltodextrins at 70 degrees C as primary products, suggested significant applications for the enzyme in starch-processing industries.
authors.affiliation: Department of Food Microbiology, Central Food Technological Research Institute, Mysore, India.
authors.forename: C
authors.initials: C
authors.lastname: Suresh
authors.forename: A K
authors.initials: AK
authors.lastname: Dubey
authors.forename: S
authors.initials: S
authors.lastname: Srikanta
authors.forename: S U
authors.initials: SU
authors.lastname: Kumar
authors.forename: N G
authors.initials: NG
authors.lastname: Karanth
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D013696:Temperature
chemical_list: D013213:Starch; D006867:Hydrolases