title: Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms.
abstract: The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom.
authors.forename: F
authors.initials: F
authors.lastname: Floret
authors.forename: L C
authors.initials: LC
authors.lastname: Chaudhary
authors.forename: W C
authors.initials: WC
authors.lastname: Ellis
authors.forename: S
authors.initials: S
authors.lastname: El Hassan
authors.forename: N
authors.initials: N
authors.lastname: McKain
authors.forename: C J
authors.initials: CJ
authors.lastname: Newbold
authors.forename: R J
authors.initials: RJ
authors.lastname: Wallace
mesh_terms: D000821:Animal Feed; D000818:Animals; D001384:Azetidines / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D001713:Biphenyl Compounds / Q000494:pharmacology*; D003013:Clostridium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D003641:Deamination; D010450:Endopeptidases / Q000378:metabolism*; D004791:Enzyme Inhibitors; D009861:Onium Compounds / Q000494:pharmacology*; D006109:Poaceae; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D011759:Pyrrolidines / Q000494:pharmacology*; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*; D012756:Sheep
chemical_list: C121134:1-(2-(2-methyl-4-nitrophenyl)diaz-1-enyl)pyrrolidine-2-carboxylic acid; D001384:Azetidines; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D009861:Onium Compounds; D011506:Proteins; D011759:Pyrrolidines; C031291:diphenyliodonium; D011392:Proline; D010450:Endopeptidases