Induced mono-(ADP)-ribosylation of rat liver cytosolic proteins by lipid peroxidant agents.
We have studied the effect of free radical generating agents on the mono-(ADP)-ribosylation of rat liver cytosolic proteins. Our results show that this post-translational modification, whose physiological significance is still unclear, is activated by lipid peroxidant agents via activation of cytoplasmatic mono-(ADP)-ribosyltransferases. The implication of free radicals in this process is demonstrated by the fact that mono-(ADP)-ribosylation can be prevented by melatonin, N-tert-butyl-alpha-phenylnitrone and dithiothreitol. On the basis of our results, we discuss the modification of proteins caused by free radicals as a possible mechanism by which they damage the cell.
Parrado
J
J
Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, Spain.
Bougria
M
M
Ayala
A
A
Machado
A
A
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001555:Benzene Derivatives / Q000633:toxicity; D003497:Cyclic N-Oxides; D003600:Cytosol / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology; D009536:Niacinamide / Q000378:metabolism; D009589:Nitrogen Oxides / Q000494:pharmacology; D016877:Oxidants / Q000633:toxicity*; D010269:Paraquat / Q000633:toxicity; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D001555:Benzene Derivatives; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D005609:Free Radicals; D009589:Nitrogen Oxides; D016877:Oxidants; D011506:Proteins; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; C029217:phenyl-N-tert-butylnitrone; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; D008550:Melatonin; C007164:cumene hydroperoxide; D010269:Paraquat; D004229:Dithiothreitol