[Detection of circulating autoantibodies to beta 2-adrenergic receptors in patients with asthma].
To understand the roles of autoantibodies to beta 2 adrenergic receptors in the pathogenesis of asthma, we investigated the positive chronotropic action of beta 2-selective adrenergic agonist, clenbuterol, on cultured neonatal rat cardiomyocytes. Thereafter, we detected the autoantibodies to beta 2-adrenergic receptors in the sera from patients with asthma as it could inhibit the positive chronotropic action of clenbuterol. In the sera of all patients with asthma (16 cases) there were autoantibodies to beta 2-adrenergic receptors, but not in the normal controls (20 cases). Further study showed that the inhibitory autoantibodies were IgG type. This experiment suggests that the autoantibodies to beta 2-adrenergic receptors may play an important role in the pathogenesis of asthma.
Wu
J
J
First Affiliated Hospital, Nanjing Medical University.
Yin
K
K
Yang
Y
Y
D000319:Adrenergic beta-Antagonists; D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D001249:Asthma / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D002478:Cells, Cultured; D002976:Clenbuterol / Q000037:antagonists & inhibitors; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018343:Receptors, Adrenergic, beta-2 / Q000276:immunology*
D000319:Adrenergic beta-Antagonists; D001323:Autoantibodies; D007106:Immune Sera; D018343:Receptors, Adrenergic, beta-2; D002976:Clenbuterol