title: Internal initiation of translation of bovine viral diarrhea virus RNA.
abstract: Initiation of translation on the bovine viral diarrhea virus (BVDV) internal ribosomal entry site (IRES) was reconstituted in vitro from purified translation components to the stage of 48S ribosomal initiation complex formation. Ribosomal binding and positioning on this mRNA to form a 48S complex did not require the initiation factors eIF4A, eIF4B, or eIF4F, and translation of this mRNA was resistant to inhibition by a trans-dominant eIF4A mutant that inhibited cap-mediated initiation of translation. The BVDV IRES contains elements that are bound independently by ribosomal 40S subunits and by eukaryotic initiation factor (eIF) 3, as well as determinants that mediate direct attachment of 43S ribosomal complexes to the initiation codon.
authors.affiliation: Morse Institute for Molecular Genetics, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, Brooklyn, New York, 11203, USA.
authors.forename: T V
authors.initials: TV
authors.lastname: Pestova
authors.forename: C U
authors.initials: CU
authors.lastname: Hellen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D018387:Codon, Initiator; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics*; D039621:Eukaryotic Initiation Factor-3; D039601:Eukaryotic Initiation Factor-4A; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D018387:Codon, Initiator; D039621:Eukaryotic Initiation Factor-3; D010448:Peptide Initiation Factors; D012367:RNA, Viral; D039601:Eukaryotic Initiation Factor-4A