title: Correlation between DNA content and p53 deletion in colorectal cancer.
abstract: OBJECTIVE
  To find out whether tumour DNA content correlates with allelic loss of p53 and other pathological features in primary colorectal carcinomas.
  
  
  METHODS
  Ongoing prospective study.
  
  
  METHODS
  University hospital, Italy.
  
  
  METHODS
  128 patients who had undergone radical resections for colorectal carcinoma.
  
  
  METHODS
  Flow cytometric measurement of tumour DNA content and detection of allelic loss on the short arm of chromosome 17 by Southern blot (restriction fragment length polymorphism) analysis in fresh tumour specimens.
  
  
  METHODS
  Correlation between DNA ploidy and deletion of p53, as well as between these two genetic events and clinicopathological variables.
  
  
  RESULTS
  Interpretable DNA histograms were obtained for 122 tumour specimens. Forty-three tumours (35%) were diploid and 79 (65%) aneuploid. The diploid tumours were significantly more common in the proximal colon (from the caecum to the splenic flexure) than in the distal colon (from the descending colon to the rectum) (p = 0.002). The allelic state on the short arm of chromosome 17 was evaluated in 80 heterozygous patients. Forty-four tumour specimens (55%) showed deletion of 17p. Allelic loss of p53 was significantly more common in the distal and rectal tumours than in the proximal ones (p < 0.0001). Aneuploidy was more common among those tumours which had shown deletion of p53 than in those that had not (p = 0.0008).
  
  
  CONCLUSIONS
  DNA aneuploidy was significantly associated with the deletion of the p53 gene. This suggests that the functional loss of p53 may favour the growth and establishment of an aneuploid cell population within tumours. Tumours of the proximal and distal colon differ in their genetic nature.
authors.affiliation: Clinica Chirurgica Generale, Università di Firenze, Florence, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Cianchi
authors.forename: M
authors.initials: M
authors.lastname: Balzi
authors.forename: A
authors.initials: A
authors.lastname: Becciolini
authors.forename: V
authors.initials: V
authors.lastname: Giachè
authors.forename: L
authors.initials: L
authors.lastname: Messerini
authors.forename: A
authors.initials: A
authors.lastname: Palomba
authors.forename: E
authors.initials: E
authors.lastname: Tisti
authors.forename: P
authors.initials: P
authors.lastname: Faraoni
authors.forename: F
authors.initials: F
authors.lastname: Chellini
authors.forename: F
authors.initials: F
authors.lastname: Pucciani
authors.forename: G
authors.initials: G
authors.lastname: Perigli
authors.forename: C
authors.initials: C
authors.lastname: Cortesini
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D002288:Adenocarcinoma, Mucinous / Q000235:genetics; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D015139:Blotting, Southern; D002886:Chromosomes, Human, Pair 17; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D017353:Gene Deletion; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
chemical_list: D004273:DNA, Neoplasm