title: Cell-surface glycoprotein of oligodendrocyte progenitors involved in migration.
abstract: Myelination by oligodendrocytes in the CNS involves the migration to and recognition and ensheathment of axons. These distinct developmental phases of myelination are assumed to involve the interplay of a precisely regulated set of cell adhesion molecules expressed by both neurons and glial cells. These molecules remain largely unelucidated. In this paper we have identified a large (330 kDa) glycoprotein expressed by murine oligodendrocyte progenitor cells in vitro and in vivo that is downregulated as oligodendrocytes mature. Antigen-positive oligodendrocyte progenitor cells purified by panning develop into myelin-associated glycoprotein-positive oligodendrocytes and also adhere to cultured neurons. Polyclonal antibodies directed against the protein reduce the migration of oligodendrocyte progenitor cells. The observations suggest that the AN2 antigen may play a role in early stages of myelination.
authors.affiliation: Department of Neurobiology, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Niehaus
authors.forename: J
authors.initials: J
authors.lastname: Stegmüller
authors.forename: M
authors.initials: M
authors.lastname: Diers-Fenger
authors.forename: J
authors.initials: J
authors.lastname: Trotter
mesh_terms: D000818:Animals; D000906:Antibodies; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology; D001921:Brain / Q000166:cytology / Q000196:embryology; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009186:Myelin Sheath / Q000502:physiology; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D009836:Oligodendroglia / Q000737:chemistry* / Q000166:cytology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology
chemical_list: D000906:Antibodies; D008562:Membrane Glycoproteins