title: Activation of mitogen-activated protein kinase by the bradykinin B2 receptor is independent of receptor phosphorylation and phosphorylation-triggered internalization.
abstract: Recent evidence suggests that serine/threonine phosphorylation and internalization of beta2-adrenergic receptors play critical roles in signalling to the mitogen-activated protein kinase cascade. To investigate whether this represents a general mechanism employed by G protein-coupled receptors, we studied the requirement of these processes in the activation of mitogen-activated protein kinase by G alpha(q)-coupled bradykinin B2 receptors. Mutant B2 receptors impaired in receptor phosphorylation and internalization are fully capable to activate mitogen-activated protein kinase. Bradykinin-induced long-term effects on mitogenic signalling monitored by measuring the transcriptional activity of Elk1 were identical in cells expressing the wild-type or mutant B2 receptors. Therefore, G protein-coupled bradykinin receptors activate the mitogen-activated protein kinase pathway independently of receptor phosphorylation and internalization.
authors.affiliation: Ludwig Institute for Cancer Research, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Blaukat
authors.forename: A
authors.initials: A
authors.lastname: Pizard
authors.forename: R M
authors.initials: RM
authors.lastname: Rajerison
authors.forename: F
authors.initials: F
authors.lastname: Alhenc-Gelas
authors.forename: W
authors.initials: W
authors.lastname: Müller-Esterl
authors.forename: I
authors.initials: I
authors.lastname: Dikic
mesh_terms: D001920:Bradykinin / Q000494:pharmacology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002460:Cell Line; D004789:Enzyme Activation; D006801:Humans; D010766:Phosphorylation; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D001920:Bradykinin