title: Cloning of the cDNA encoding phenylalanyl tRNA synthetase regulatory alpha-subunit-like protein whose expression is down-regulated during differentiation.
abstract: Hybrid polar compounds (HPCs), such as suberoylanilide hydroxamic acid (SAHA), induce differentiation of transformed cells. Differential display of RNA was used to identify genes whose expression is changed during SAHA-induced differentiation of murine erythroleukemia (MEL) cells. One such cDNA was identified whose mRNA level decreased by 50% after 8h of SAHA treatment as determined by Northern blot analysis. The full-length cDNA (1944bp in length) was cloned by sequencing of an EST clone and rapid amplification of 5' cDNA ends (5'-RACE). The predicted amino acid sequence is 589 amino acids and shares 45% identity with the yeast cytoplasmic phenylalanyl tRNA synthetase (PheRS) regulatory alpha-subunit. Human EST clones which share over 90% identity of predicted amino acid sequence with this cDNA map to chromosome 2 near the paired box homeotic gene 3 (PAX3) locus, a region syngenic to mouse chromosome 1. This is the first report of the cloning of the full-length cDNA for the murine PheRS regulatory alpha-subunit-like protein. The level of PheRS alpha-subunit-like mRNA is regulated during differentiation but not during cell cycle progression.
authors.affiliation: Cell Biology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center and Graduate School of Medical Sciences, Cornell University Medical School, New York, NY 10021, USA. x-zhou@ski.mskcc.org
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: V M
authors.initials: VM
authors.lastname: Richon
authors.forename: L
authors.initials: L
authors.lastname: Ngo
authors.forename: R A
authors.initials: RA
authors.lastname: Rifkind
authors.forename: P A
authors.initials: PA
authors.lastname: Marks
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015536:Down-Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D010652:Phenylalanine-tRNA Ligase