Enhancement of Mycoplasma bovis detection in milk samples by antigen capture prior to PCR.
A rapid and sensitive procedure to detect Mycoplasma bovis from milk samples was developed. Mycoplasma cells were collected by selective binding to a monoclonal antibody, thus separating them from milk proteins and facilitating DNA extraction. Polymerase chain reaction (PCR) using species-specific primers allowed detection of 20 cfu ml-1 in agarose gels and 2 cfu ml-1 on Southern blots. The method is especially suitable for specimens from subclinical cases and intermittent shedders.
Hotzel
H
H
Division 4, Federal Institute for Health Protection of Consumers and Veterinary Medicine, Naumburger Str. 96a, D-07743 Jena, Germany.
Heller
M
M
Sachse
K
K
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D008892:Milk / Q000382:microbiology*; D009174:Mycoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D000954:Antigens, Surface