Insulin sensitivity, clearance and release in kininogen-deficient rats.
Insulin sensitivity of kininogen-deficient rats was compared with that of normal rats using euglycaemic hyperinsulinaemic glucose clamping. Anaesthetized animals were infused with 2-50 mU kg-1 min-1 of insulin and the glucose infusion rates needed to maintain euglycaemia were determined. Maximum glucose uptake, insulin sensitivity index and insulin clearance were reduced in kininogen-deficient rats. Captopril increased the amount of glucose needed to maintain euglycaemia during infusion of 2 and 10 mU kg-1 min-1 of insulin in normal rats, but had no effect in kininogen-deficient rats. Anaesthetized rats of both strains were given an intraperitoneal injection of glucose and the evolution of blood glucose was followed for 120 min. The peak increase was higher in kininogen-deficient rats. Similar larger increases in blood glucose were observed after glucose injection in normal rats previously treated with HOE 140, a bradykinin B2 receptor antagonist. After glucose injection, plasma insulin increased in both groups of rats but reached lower levels in kininogen-deficient animals. These results suggest that bradykinin is involved not only in the clearance of glucose and insulin by the tissues during insulin infusion but also that bradykinin can affect the release of insulin after a glucose load.
Damas
J
J
Department of Human Physiology, Nutrition and Metabolic Disorders, Faculty of Medicine, University of Liege, B-4020, Liege, Belgium. jacques.damas@ulg.ac.be
Bourdon
V
V
Lefebvre
P J
PJ
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology; D065168:Bradykinin Receptor Antagonists; D002216:Captopril / Q000494:pharmacology; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D000078790:Insulin Secretion; D007704:Kininogens / Q000172:deficiency*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D043782:Receptor, Bradykinin B2
D001786:Blood Glucose; D065168:Bradykinin Receptor Antagonists; D007328:Insulin; D007704:Kininogens; D043782:Receptor, Bradykinin B2; C065679:icatibant; D002216:Captopril; D001920:Bradykinin