title: Determination of buspirone and 1-(2-pyrimidinyl)-piperazine (1-PP) in human plasma by capillary gas chromatography.
abstract: Two separate gas chromatographic methods for the determination of buspirone and its active metabolite, 1-(2-pyrimidinyl)-piperazine (1-PP) in human plasma are described. Both procedures involve solid-phase extraction (the packing material of the cartridges used was C8 for buspirone and a mixed-mode sorbent for 1-PP), injection of the sample into a gas chromatograph equipped with a fused-silica capillary column and a nitrogen-phosphorus detector, and analysis with temperature programming (from 220 degrees C to 285 degrees C for buspirone and from 138 degrees C to 285 degrees C for 1-PP). The coating material of the analytical column was 5% diphenyl dimethyl silicone for buspirone and 50% diphenyl dimethyl silicone for 1-PP. Zolpidem was used as an internal standard in the buspirone assay and 1-phenylpiperazine in the 1-PP assay. Recovery of buspirone and 1-PP averaged 98% and 89%, respectively, and the limit of quantification was 0.2 ng/mL for both compounds. The between-run coefficients of variation ranged from 3.2% to 9.4% and from 2.9% to 8.6% for samples spiked with three different concentrations of buspirone and 1-PP, respectively. The suitability of these assays for pharmacokinetic studies was shown by analyzing timed plasma samples from volunteers after ingestion of a single therapeutic dose of buspirone (10 mg).
authors.affiliation: Department of Clinical Pharmacology, University of Helsinki and Helsinki University Central Hospital, Finland.
authors.forename: K T
authors.initials: KT
authors.lastname: Kivistö
authors.forename: J
authors.initials: J
authors.lastname: Laitila
authors.forename: K
authors.initials: K
authors.lastname: Mårtensson
authors.forename: P J
authors.initials: PJ
authors.lastname: Neuvonen
mesh_terms: D014151:Anti-Anxiety Agents / Q000097:blood*; D002065:Buspirone / Q000031:analogs & derivatives* / Q000097:blood*; D002138:Calibration; D002849:Chromatography, Gas; D006801:Humans; D015203:Reproducibility of Results; D017366:Serotonin Receptor Agonists / Q000097:blood*
chemical_list: D014151:Anti-Anxiety Agents; D017366:Serotonin Receptor Agonists; C037921:1-(2-pyrimidinyl)piperazine; D002065:Buspirone