title: CD5 and CD23 expression on B cells in peripheral blood and synovial fluid of rheumatoid arthritis patients: relationship with interleukin-4, soluble CD23 and tumour necrosis factor alpha levels.
abstract: METHODS
  We have studied in peripheral blood (PB) and synovial fluid (SF) of 31 patients diagnosed with rheumatoid arthritis (RA), the expression of CD5 and CD23 antigens on B cells, and the levels of soluble CD23 (sCD23), interleukin-4 (IL-4) and tumour necrosis factor alpha (TNF-alpha). We have also correlated the results with the disease activity index.
  
  
  RESULTS
  CD5+ B cells are expanded in SF and, moreover, show higher expression of CD23 than CD5 - B cells. Twelve patients had detectable levels of IL-4 in plasma and 10 in SF (nine patients in both samples); the absence of IL-4 was related to a higher expression of CD23 on CD5 + B cells and with higher levels of sCD23. A negative correlation was found in SF between TNF-alpha and sCD23 levels.
  
  
  CONCLUSIONS
  There is no correlation between disease activity index and the different parameters studied (expression of CD5 and CD23 on B cells, sCD23, IL-4 and TNF-alpha levels) either in plasma/PB or in SF.
authors.affiliation: Department of Rheumatology, Hospital of Navarra, Pamplona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Loza
authors.forename: T
authors.initials: T
authors.lastname: Tinturé
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-Ibarrola
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D015415:Biomarkers; D018956:CD5 Antigens / Q000032:analysis*; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000032:analysis*; D012720:Severity of Illness Index; D012995:Solubility; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018956:CD5 Antigens; D017455:Receptors, IgE; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4