Transduction of well-differentiated airway epithelium by recombinant adeno-associated virus is limited by vector entry.
The limitations of adeno-associated virus (AAV)-mediated vectors for lung-directed gene transfer were investigated by using differentiated human respiratory epithelium in air-liquid interface cultures. Transduction efficiency was high in undifferentiated cells and was enhanced in well-differentiated cells after basolateral application of the vector or after apical application following disruption of tight junctions or pretreatment of the cultures with glycosidases. These results indicate that transduction of airway epithelia by AAV vectors is limited by entry and reinforce the importance of a physical barrier on the airway surface.
Bals
R
R
Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Xiao
W
W
Sang
N
N
Weiner
D J
DJ
Meegalla
R L
RL
Wilson
J M
JM
D002454:Cell Differentiation; D002472:Cell Transformation, Viral; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology*; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000502:physiology*; D006026:Glycoside Hydrolases / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008168:Lung / Q000166:cytology / Q000821:virology*; D011995:Recombination, Genetic; D014357:Trypsin / Q000378:metabolism
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D006026:Glycoside Hydrolases; D014357:Trypsin