[A study of a derivative of Taq DNA polymerase].
Using the method of double primer oligonucleotide-mediated mutagenesis, the high expression plasmid of TaqND236, a derivative of Taq DNA polymerase, was constructed. To determine the frameshift mutation frequency of the in vitro DNA synthesis, we constructed a Gapped-DNA system using the pFDPM118 (a mutant of pUC118 with a -1 frameshift mutation on the lacZ gene) as template. By calculating the ratio of blue and white colonies on the X-gal plate after transforming E. coli TG1, the frameshift mutation frequency of Taq and TaqND236 was measured. It was found that the replication fidelity of the deleted Taq-TaqND236 increased more than 10 folds.
Zhang
J
J
Institute of Genetics, Fudan University, Shanghai.
Ji
C N
CN
Du
H S
HS
Zheng
Z H
ZH
Mao
Y M
YM
D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D016368:Frameshift Mutation; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism*
D019914:Taq Polymerase