title: Amphiphilic diblock copolymeric nanospheres composed of methoxy poly(ethylene glycol) and glycolide: properties, cytotoxicity and drug release behaviour.
abstract: Amphiphilic diblock copolymers based on methoxy poly(ethylene glycol) (MePEG) and glycolide with different molar composition were synthesized by bulk polymerization without any catalysts. Using diblock copolymers, we have prepared indomethacin-loaded polymeric nanospheres by forming a micelle in selective solvents. The size of nanospheres measured using dynamic light scattering exhibited a narrow monodisperse size distribution and an average diameter in the range of less than 200 nm. The critical micelle concentration (CMC) of MG70 sample determined by fluorescence spectroscopy was 1.57 x 10(-7) mol/l which was lower than the CMC of common low molecular weight surfactant. In vitro release experiments using indomethacin-loaded MePEG/glycolide nanospheres exhibited the sustained release behaviour without any burst effect. In addition, the results of cytotoxicity tests using an MTT assay method showed that these MePEG/glycolide nanospheres could remarkably reduce cell damage compared with unloaded free drug.
authors.affiliation: Department of Industrial Chemistry, College of Engineering, Hanyang University, Seoul, South Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: I G
authors.initials: IG
authors.lastname: Shin
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018360:Crystallography, X-Ray; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000008:administration & dosage / Q000493:pharmacokinetics; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D011100:Polyglycolic Acid; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D007213:Indomethacin