Analysis of the mouse MAP1B gene identifies a highly conserved 4.3 kb 3' untranslated region and provides evidence against the proposed structure of DBI-1 cDNA.
We determined the previously unknown 3' end of MAP1B mRNA. We found an unusually long and highly conserved 3' untranslated region (3'UTR) of 4.3 kb and detected a polymorphism in the 3' flanking region probably due to the integration of an endogenous retroviral MuERV-L element. Furthermore, we found that MAP1B 3'UTR overlapped with the 5' end of the cDNA encoding DBI-1. However, further analysis suggested that the published structure of DBI-1 cDNA is most likely the result of fortuitous joining of unrelated cDNA fragments during cloning.
Meixner
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A
Institute of Biochemistry and Molecular Cell Biology, Vienna Biocenter, University of Vienna, Dr. Bohr-Gasse 9, A-1030, Vienna, Austria.
Wiche
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G
Propst
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F
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D053487:DEAD-box RNA Helicases; D018076:DNA, Complementary / Q000737:chemistry; D005091:Exons; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface / Q000235:genetics*; D017385:Sequence Homology; D025521:Tumor Suppressor Proteins; D020506:Untranslated Regions / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; C110734:Ints6 protein, mouse; D008869:Microtubule-Associated Proteins; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D020506:Untranslated Regions; C085284:microtubule-associated protein 1B; D053487:DEAD-box RNA Helicases