Prothiofos metabolites in human poisoning.
METHODS
A 63-year-old woman was admitted to a local hospital after the ingestion of 40% prothiofos preparation (Tokuthion) 370 mL. Gastric lavage was performed and cathartics, active charcoal, diuretics, atropine sulfate, and pralidoxime were administered. Serum cholinesterase activity was 1.3 IU/L (normal 200-460 IU/L). The patient's consciousness was gradually restored after 4 hours of charcoal hemoperfusion and she was discharged 5 days after admission with no sequelae.


METHODS
Plasma and urine prothiofos and metabolites were detected by gas chromatography-flame photometry and gas chromatography-mass spectrometry. Two despropyl metabolites were synthesized for identification and estimation.


RESULTS
The main metabolites were identified with authentic prothiofos and methyl esters of synthesized des-S-propyl prothiofos oxon (O-2,4-dichlorophenyl O-ethyl phosphate), despropyl prothiofos oxon (O-2,4-dichlorophenyl O-ethyl phospholothiolate), and des-S-propyl prothiofos (O-2,4-dichlorophenyl O-ethyl phosphorothioate). Despropyl prothiofos (O-2,4-dichlorophenyl O-ethyl phosphorodithioate) was also identified in plasma. Large amounts of the hydrolyzed product, 2,4-dichlorophenol and its conjugate were also found. The metabolic pattern of prothiofos in humans appears to be different from that in rats.
Sakata
M
M
Health Sciences University of Hokkaido, Nayoro City Hospital, Japan. msakata@hoku-iryo-u.ac.jp
Gotoh
M
M
Ubukata
K
K
Hayashi
H
H
Kotaki
M
M
Omote
T
T
D002606:Charcoal / Q000627:therapeutic use; D002800:Cholinesterase Inhibitors / Q000097:blood / Q000378:metabolism* / Q000506:poisoning* / Q000652:urine; D002849:Chromatography, Gas; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007306:Insecticides / Q000097:blood / Q000378:metabolism* / Q000506:poisoning* / Q000652:urine; D008745:Methylation; D008875:Middle Aged; D062025:Organophosphate Poisoning; D063086:Organothiophosphates / Q000097:blood / Q000378:metabolism* / Q000652:urine
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D063086:Organothiophosphates; D002606:Charcoal; C032072:prothiophos