The number of interleukin 1 receptors on lung fibroblasts in patients with idiopathic pulmonary fibrosis.
BACKGROUND
We investigated the expression of the IL-1 receptor on lung fibroblasts in patients with idiopathic pulmonary fibrosis (IPF) the histology of which was diagnosed as usual interstitial pneumonia.


METHODS
125I-labeled IL-1alpha bound to lung fibroblasts in a specific and saturable manner in lung fibroblasts obtained from both IPF patients and control subjects.


RESULTS
Scatchard plot analysis revealed a single type of binding site in both groups. In total, 8,600 +/- 1,450 (mean +/-SD) sites/cell with a dissociation constant (Kd) of 1.83 +/- 0.28 (mean +/-SD) nM in patients with IPF and 7,920 +/- 1,470 sites/cell with a Kd of 2.43 +/- 0.49 nM in control subjects were counted. Furthermore, affinity cross-linking experiments revealed that the IL-1 receptor on lung fibroblasts in patients with IPF and control subjects had a molecular size of 80 kD.


CONCLUSIONS
In conclusion, type I receptor was present on human lung fibroblasts, but there were no differences in number or affinity between IPF patients and controls.
Takeuchi
M
M
Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kyoto, Japan. mtakex@cc.kyoto-su.ac.jp
Nagai
S
S
Tsutumi
T
T
Mio
T
T
Izumi
T
T
D000368:Aged; D001665:Binding Sites / Q000502:physiology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D008970:Molecular Weight; D011658:Pulmonary Fibrosis / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins
D007375:Interleukin-1; D007457:Iodine Radioisotopes; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins