title: [Inhibitory effect of replication and expression of HDV by antisense oligodeoxynucleotides in H1 delta 9 cell].
abstract: OBJECTIVE
  To study inhibitory effect of an antisense oligodeoxynucleotide (ASODN) and its phosphorothioate (S-ASODN) on replication and expression of HDV in H1 delta 9 cell.
  
  
  METHODS
  In previous studies, it was proved that the ASODNs which are complementary to genomic HDV ribozyme self-cleavage site and stem I regions can inhibit availably genomic HDV ribozyme activity. In the present study, a 15-mer ASODN and S-ASODS which are complementary to this region (nucleotide 684-698) were added to medium of cultured H1 delta 9 cell. HDAg and HDV cDNA were detected by ELISA and Dot blot hybridization.
  
  
  RESULTS
  Twenty-fourth hour after 6 mumol/L ASODN and S-ASODN were added, both the secreting amount of HDAg in the supernatant and HDV RNA were inhibited. The inhibiting rates were 76.14% and 84.50% respectively. When the concentration of S-ASODN was 2, 4, 6 mumol/L, the inhibiting rate showed the feature of dosage dependence. Inhibitory effect of ASODN and S-ASODN were similar in the same dosage.
  
  
  CONCLUSIONS
  The results suggest that ASODN and S-ASODN can availably inhibit replication and expression of HDV in H1 delta 9 cell.
authors.affiliation: Center of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Mao
authors.forename: Q
authors.initials: Q
authors.lastname: Li
mesh_terms: D003698:Hepatitis Delta Virus / Q000187:drug effects* / Q000502:physiology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012367:RNA, Viral / Q000037:antagonists & inhibitors; D013873:Thionucleotides / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D016376:Oligonucleotides, Antisense; D012367:RNA, Viral; D013873:Thionucleotides