title: Influence of perioperative whole blood transfusions on lymphocyte subpopulations in patients with stage II breast cancer.
abstract: Preliminary reports have suggested an adverse relationship between blood transfusion and survival after surgery in patients with solid tumour. One might postulate that from these studies, perioperative blood transfusions alter host immune defences. We therefore examined the influence of homologous whole blood transfusion on circulating lymphocyte subpopulations in transfused patients compared with non-transfused patients. Fifty-one women with Stage II breast cancer who underwent surgical procedures were studied. Patients were classified into two groups on the basis of whether or not they had received blood transfusion. The lymphocyte subpopulations were analyzed by flow cytometry before cancer surgery and three weeks after the operation. CD3+, CD4+, CD8+, and CD20+ cells as the lymphocyte subsets were quantitated using appropriate monoclonal antibodies. No significant differences between pre- and postoperative lymphocyte subset levels were seen in non-transfused patients. However, there was a statistically significant increase in the CD8+ cell count; decreasing CD4+ cell count and decreased CD3+ cells levels were observed in the transfused group (P < 0.05). Although these early results of the study suggest that the blood transfusions could be associated with alterations in lymphocyte populations, additional studies are needed to elucidate the possible mechanism of the transfusion-induced immunological modulations.
authors.affiliation: Department of Surgical Oncology, Ankara University, School of Medicine, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Eroğlu
authors.forename: H
authors.initials: H
authors.lastname: Canpinar
authors.forename: E
authors.initials: E
authors.lastname: Kansu
mesh_terms: D001803:Blood Transfusion; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets; D009367:Neoplasm Staging; D019990:Perioperative Care