Examination of the relationship between parameters to determine electropermeability of Saccharomyces cerevisiae.
A rectangular electric pulse was applied to Saccharomyces cerevisiae suspensions in NaCl solutions. The relationship between field strength, pulse width and conductivity of extracellular media of key--factors to determine the yield of electropermeability--was examined at the time when the same permeability occurred. The results showed that the dependence of the yield of permeability upon the width of applied pulse was mutually related with the conductivity of extracellular media. Namely at one field strength, the value of pulse width is inversely proportional to that of conductivity of media and its relationship holds true for any field strength. Further, the relationship between parameters considered bears a close resemblance to that recognized between stress amplitude and the number of cycles to failure in the fatigue fracture of materials.
Muraji
M
M
Faculty of Engineering, Osaka City University, Osaka, Japan.
Taniguchi
H
H
Tatebe
W
W
Berg
H
H
D002463:Cell Membrane Permeability; D004560:Electricity; D012441:Saccharomyces cerevisiae / Q000502:physiology*