Presence of high levels of MT1-MMP protein in fibroblastic cells of human invasive carcinomas.
Matrix metalloproteinase 2 (MMP2) activity is associated with the aggressiveness of human cancers. Therefore, the mechanisms regulating its activation are of great interest for a better understanding of malignant invasive processes. MT1-MMP, a membrane-bound MMP, is involved in the conversion of the latent form of MMP2 to the active one. In the present study, we have raised 3 monoclonal antibodies (Mabs) directed against 3 different epitopes of human MT1-MMP, which we used to investigate the expression and cellular localization of MT1-MMP protein in human carcinomas. MT1-MMP protein was present in all invasive carcinomas tested, and it was almost exclusively located to the stromal cells and not to cancer cells as previously reported, suggesting that MMP2 activation might be a peri-fibroblastic event.
Chenard
M P
MP
Service d'Anatomie Pathologique Générale, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, France.
Lutz
Y
Y
Mechine-Neuville
A
A
Stoll
I
I
Bellocq
J P
JP
Rio
M C
MC
Basset
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D019556:COS Cells; D002277:Carcinoma / Q000201:enzymology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology / Q000473:pathology; D018275:Carcinoma, Lobular / Q000201:enzymology / Q000473:pathology; D004789:Enzyme Activation; D004790:Enzyme Induction; D000939:Epitopes / Q000276:immunology; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D053511:Matrix Metalloproteinase 14; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D017154:Stromal Cells / Q000201:enzymology; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; C507096:Mmp14 protein, mouse; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D053511:Matrix Metalloproteinase 14