Cytogenetic and molecular characterization of T-cell acute lymphoblastic leukemia as a second tumor after anaplastic large-cell lymphoma in a boy.
We report a case of acute T-cell lymphoblastic leukemia which developed in a boy 8.5 years after successful treatment for anaplastic large-cell lymphoma. Cytogenetic and molecular characterizations of the second tumor were performed. The cytogenetic investigation revealed a complex pattern of karyotypic alterations, including double minutes, ring chromosomes, and a duplication of the p21-32 region of chromosome 1. The microsatellite DNA analysis excluded rearrangement or deletion of the TAL1 gene in the tumor cells; rearrangements of the MLL gene were excluded by Southern blot analysis. To the best of our knowledge, this is the first report of T-cell lymphoblastic leukemia arising after treatment of CD 30+ anaplastic large-cell lymphoma. The different T-cell receptor rearrangement evidenced in the two tumors indicates that this second malignancy most likely emerged de novo, but was plausibly related to the previous radiation and chemotherapy.
Perotti
D
D
Division of Pediatric Oncology, Istituto Nazionale Tumori, via G. Venezian 1, 20133 Milan, Italy. perotti@istitutotumori.mi.it
Sozzi
G
G
Ferrari
A
A
Casanova
M
M
Gambirasio
F
F
Mondini
P
P
Mezzelani
A
A
Giardini
R
R
Pettenella
F
F
Papini
D
D
Biondi
A
A
Fossati-Bellani
F
F
Massimino
M
M
D002648:Child; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007621:Karyotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000532:radiotherapy; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000209:etiology; D016609:Neoplasms, Second Primary; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D014178:Translocation, Genetic
D016692:Receptors, Antigen, T-Cell, gamma-delta