Detection of Fas/APO-1 in six human urogenital malignant cell lines with flow cytometry.
OBJECTIVE
To investigate the expression of Fas/APO-1 in urogenital tumor cell lines.


METHODS
With direct immunofluorescence, the expression of Fas/APO-1 in six urogenital malignant cell lines and one primary in vitro cultured normal renal fibroblast was detected by flow cytometry.


RESULTS
Expression of Fas/APO-1 was detected in all six urogenital tumor cell lines, but with limited positive cell percentage and relatively lower fluorescence intensity, compared with expression of Fas/APO-1 in normal control of primary in vitro cultured renal fibroblast.


CONCLUSIONS
Lower expression of Fas/APO-1 in urogenital malignant cell lines than that in normal cells might be the reason for occurrence and progression of urogenital malignant tumors.
Li
H
H
Department of Urology, First Hospital of Beijing Medical University, Beijing Medical University, China.
Yu
L
L
Guo
Y
Y
Ding
Y
Y
Liu
L
L
D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology*; D008297:Male; D011471:Prostatic Neoplasms / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000473:pathology*; D019014:fas Receptor / Q000096:biosynthesis*
D019014:fas Receptor