title: cDNA cloning and sequence analysis of bubaline growth hormone.
abstract: The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homology comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.
authors.affiliation: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Verma
authors.forename: A
authors.initials: A
authors.lastname: Ghorpade
authors.forename: G
authors.initials: G
authors.lastname: Tiwari
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.forename: L C
authors.initials: LC
authors.lastname: Garg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D013006:Growth Hormone / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D013006:Growth Hormone