title: The interaction between the archaeal elongation factor 1alpha and its nucleotide exchange factor 1beta.
abstract: In Sulfolobus solfataricus the binding of the exchange factor 1beta (SsEF-1beta) to SsEF-1alpha-GDP displaces the nucleotide and the SsEF-1alpha-SsEF-1beta complex is formed. The complex itself is stable, but it dissociates upon the addition of GDP or Gpp(NH)p but not ATP. Since the rate of the formation of the SsEF-1alpha-SsEF-1beta complex is significatively slower than the rate of the nucleotide exchange catalyzed by SsEF-1beta it can be inferred that in vivo the GDP/GTP exchange reaction proceeds via an SsEF-1alpha-SsEF-1beta interaction without involving the formation of a stable binary complex as an intermediate.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Raimo
authors.forename: M
authors.initials: M
authors.lastname: Masullo
authors.forename: V
authors.initials: V
authors.lastname: Bocchini
mesh_terms: D020662:Guanine Nucleotide Exchange Factors; D007700:Kinetics; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D016944:Sulfolobus / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins