Quantification of residual dimethyl sulfoxide in supernatants of haematopoietic stem cells by capillary zone electrophoresis.
Dimethyl sulfoxide (DMSO) is a chemical compound that is used to preserve haematopoietic stem cells during freezing at -180 degrees C. As DMSO is largely removed by washing before reinjection of cells into a patient, accidents (notably cardiovascular) are infrequent. The lack of a method for evaluating the residual quantities of this product led us to develop a technique for assaying DMSO by capillary zone electrophoresis without extraction. This simple, rapid and precise technique was applied to the supernatant of cell pellets of thirteen patients before and after washing.
Houzé
P
P
Laboratoire de Biochimie, Hôpital Saint Louis, Paris, France.
Dal Cortivo
L
L
Anselme
M
M
Bousquet
B
B
Gourmel
B
B
D003470:Culture Media / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003470:Culture Media; D004121:Dimethyl Sulfoxide