title: Strain-specific differences in the effect of influenza A virus neuraminidase on vector-expressed hemagglutinin.
abstract: In order to evaluate the efficiency of the removal of sialic acid residues from the influenza virus hemagglutinin by the viral neuraminidase in the course of the virus replication cycle, CV-1 cells expressing the hemagglutinin of H7 subtype from an SV40-based vector were superinfected with influenza virus strain A/Duck/Ukraine/63 (H3N8) or A/USSR/90/77 (H1N1). Vector-expressed hemagglutinin was immunoprecipitated from cell lysates and analyzed by polyacrylamide gel electrophoresis. The data indicate that the removal of sialic acid residues from the vector-expressed H7 hemagglutinin by N1 neuraminidase of A/USSR/90/77 virus in the course of the virus replication cycle is incomplete. The results are discussed in connection with previously published data showing that the low activity of NA in wild-type influenza virus results in incomplete removal of sialic acid residues from virion components.
authors.affiliation: Institut für Virologie, Philipps Universität Marburg, Germany.
authors.forename: N V
authors.initials: NV
authors.lastname: Kaverin
authors.forename: H D
authors.initials: HD
authors.lastname: Klenk
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004372:Ducks; D005822:Genetic Vectors; D015707:HN Protein / Q000096:biosynthesis / Q000235:genetics*; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D009439:Neuraminidase / Q000378:metabolism*; D013539:Simian virus 40; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D015707:HN Protein; D009439:Neuraminidase