Customisation of AFLP analysis for cassava varietal identification.
Amplified fragment length polymorphism (AFLP) markers were used in the characterization of eight cassava varieties. This nonradioactive AFLP system was customized in terms of the choice of restriction enzymes used and the selection of nucleotides added to the 3' end of primers. EcoRI/MseI and HindIII/MseI fragments generally gave monomorphic profiles while ApaI/TaqI fragments produced polymorphic profiles suggesting a genome with high G + C content. It was possible to identify the eight cassava varieties used in this study using CTG as selective bases at the TaqI primer. For cassava, the AFLP system provided a higher number of loci detected per run when compared to RAPD. The reliability accompanying AFLP analysis would thus make it suitable for the characterization of cassava varieties.
Wong
H L
HL
Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore.
Yeoh
H H
HH
Lim
S H
SH
D016172:DNA Fingerprinting / Q000379:methods; D004262:DNA Restriction Enzymes / Q000378:metabolism; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004586:Electrophoresis; D005784:Gene Amplification; D014644:Genetic Variation; D002365:Manihot / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D018744:DNA, Plant; D004262:DNA Restriction Enzymes