Induction of Toxoplasma gondii cystogenesis and multiplication arrest by treatments with a phosphatidylcholine-specific phospholipase C inhibitor.
A model of tissue cyst formation was developed using D609, a specific inhibitor of phosphatidylcholine specific-phospholipase C. The phospholipase inhibitor induced a decrease in Toxoplasma gondii multiplication and several successive treatments could lead to an arrest in parasite multiplication and full encystment of the parasites. This could be a first step towards an in vitro model of T. gondii reactivation.
Ricard
J
J
Laboratoire Relations Hôte-Agents Pathogènes, CNRS ESA 5082, Faculté de Médecine de Grenoble, La Tronche, France.
Pelloux
H
H
Gross
U
U
Meunier
A
A
Ambroise-Thomas
P
P
D000818:Animals; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
D001952:Bridged-Ring Compounds; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D010738:Type C Phospholipases