title: Regulation of the UNC-18-Caenorhabditis elegans syntaxin complex by UNC-13.
abstract: The Caenorhabditis elegans unc-13, unc-18, and unc-64 genes are required for normal synaptic transmission. The UNC-18 protein binds to the unc-64 gene product C. elegans syntaxin (Ce syntaxin). However, it is not clear how this protein complex is regulated. We show that UNC-13 transiently interacts with the UNC-18-Ce syntaxin complex, resulting in rapid displacement of UNC-18 from the complex. Genetic and biochemical evidence is presented that UNC-13 contributes to the modulation of the interaction between UNC-18 and Ce syntaxin.
authors.affiliation: Department of Nutrition, School of Medicine, Tokushima University, Tokushima 770-0042, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sassa
authors.forename: S
authors.initials: S
authors.lastname: Harada
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: J B
authors.initials: JB
authors.lastname: Rand
authors.forename: I N
authors.initials: IN
authors.lastname: Maruyama
authors.forename: R
authors.initials: R
authors.lastname: Hosono
mesh_terms: D000483:Alleles; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D005089:Exocytosis / Q000502:physiology; D017353:Gene Deletion; D015870:Gene Expression / Q000502:physiology; D017238:Genes, Helminth / Q000502:physiology; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D016254:Mutagenesis, Insertional / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism; D010641:Phenotype; D010750:Phosphoproteins; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D009435:Synaptic Transmission / Q000235:genetics; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins
chemical_list: D000954:Antigens, Surface; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D050827:Syntaxin 1; C082636:Unc-18 protein, C elegans; D033921:Vesicular Transport Proteins; C083141:phorbol ester binding protein; C494215:unc-64 protein, C elegans