title: A comparative study of rat and human Tmp21 (p23) reveals the pseudogene-like features of human Tmp21-II.
abstract: Tmp21 (p23) is involved in biosynthetic transport from the endoplasmic reticulum to the Golgi complex. We have recently characterized two cDNA-variants of human Tmp21, the Tmp21-isoforms-I and -II. Because of the lack of cDNA sequence data and protein expression data, it was not clear if Tmp21-II encodes a functional Tmp21-protein. Here we describe the cloning of the full length human Tmp21-II transcript. The putative open reading frame of Tmp21-II contains a reading frame jump and a nonsense mutation in comparison to all other Tmp21-I (p23) members. Our data indicate that hum-Tmp21-II is transcribed, but not translated. We conclude that Tmp21-II cDNA derives from a neutral pseudogene, which originates from a duplication event of the human Tmp21-isoform-I.
authors.affiliation: Institute of Physiology II, University of the Saarland, Saar, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hörer
authors.forename: R
authors.initials: R
authors.lastname: Blum
authors.forename: P
authors.initials: P
authors.lastname: Feick
authors.forename: W
authors.initials: W
authors.lastname: Nastainczyk
authors.forename: I
authors.initials: I
authors.lastname: Schulz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D018076:DNA, Complementary; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D029543:Nucleocytoplasmic Transport Proteins; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D029543:Nucleocytoplasmic Transport Proteins; D020033:Protein Isoforms; C484395:TMED10 protein, human; C484396:TMED10P1 protein, human; C100672:Tmed10 protein, rat