title: Inhibitory effect of mast cell-mediated immediate-type allergic reactions by Cichorium intybus.
abstract: We investigated the effect of an aqueous extract of Cichorium intybus (CIAE) on mast cell-mediated immediate type allergic reactions. CIAE (0.1-1000 mg kg-1) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. Especially, CIAE inhibited compound 48/80-induced anaphylactic reaction 100% with the dose of 1000 mg kg-1. CIAE 1000 mg kg-1also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with CIAE at a concentration ranging from 0.1 to 1000 mg kg-1, the plasma histamine levels were reduced in a dose-dependent manner. CIAE (1-1000 microg ml-1) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMC, when CIAE (1000 microg ml-1) was added, increased significantly compared with that of control cells. These results indicate that CIAE inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, South Korea.
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: H W
authors.initials: HW
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Lyu
authors.forename: J H
authors.initials: JH
authors.lastname: Won
authors.forename: D K
authors.initials: DK
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: E
authors.initials: E
authors.lastname: Morii
authors.forename: T
authors.initials: T
authors.lastname: Jippo
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: N H
authors.initials: NH
authors.lastname: An
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control; D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D018651:Cichorium intybus / Q000737:chemistry / Q000627:therapeutic use*; D000242:Cyclic AMP / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000188:drug therapy*; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D018926:Anti-Allergic Agents; D010936:Plant Extracts; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP