[Experimental study of myocardial myoglobin depletion induced by early myocardial infarction and coronary artery spasm].
In this study, the model of early myocardial infarction (EMI) was produced by legation of left anterior descending artery in rat. The artery spasm (CAS) was produced by external jugular vein injection of vasopressin (VP). Myocardium of apex and adjoining slice was used to make paraffin sections, then HE and LSAB-Mb staining were performed. Results showed: In 25 min after myocardial infarction, stretch-shape Mb depletion was observed in subendocardial myocardium of aortic ventricle's frontal wall. As the time of EMI prolonged, stretch-shape Mb depletion extended to epicardial layer. In CAS group, multiply, spotty Mb depletion was detected. There were more Mb depletion zones in right heart than that in left heart. The Mb depletion zones surrounded the CA or were like grape-clusters along the branches of one large CA. Thus, myocardial Mb depletion induced by EMI and CAS had different morphologic peculiarity. LSAB-Mb method could be hoped for the use of supplying objectively morphologic evidence to myocardial ischemia induced by CAS.
Zhou
W
W
Department of Forensic Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou.
D000818:Animals; D003329:Coronary Vasospasm / Q000139:chemically induced / Q000378:metabolism*; D009203:Myocardial Infarction / Q000209:etiology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009211:Myoglobin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014667:Vasopressins
D009211:Myoglobin; D014667:Vasopressins