[Promotive effect of TNF-alpha and M-CSF on osteolysis induced by giant cell tumor of bone].
OBJECTIVE
To explore the effect of cell components in giant cell tumor of bone (GCT) and cytokines expressed by them on osteolysis.


METHODS
Mononuclear stromal cells and multinucleated giant cells (MGCs) were isolated from 10 cases of GCT, and fibroblast-like stromal cells were obtained by long-term culture of mononuclear stromal cells. Osteolytic capability of above isolated cells were tested in an in vitro cell-bone resorption model.


RESULTS
All cell components isolated from GCT had capability to resorb bone matrix directly. Exogenous tumore necrosis factor-alpha (TNF-alpha) could significantly increase the bone resorption induced by both kinds of stromal cell. There were higher level of TNF-alpha and greater expression rate of macrophage colony stimulating factor (M-CSF) in GCT than in the osteosarcoma tissues or the normal serum.


CONCLUSIONS
The characteristic bone resorption behavior of GCT might be conducted by all it's three major cell components, and this bone resorption process could be promoted by both TNF-alpha and M-CSF they expressed.
Wen
J
J
Department of Pathology, Sun Yet-San University of Medical Sciences, Guangzhou.
Xie
D
D
Yao
J
J
D001859:Bone Neoplasms / Q000737:chemistry / Q000473:pathology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D018212:Giant Cell Tumor of Bone / Q000737:chemistry / Q000473:pathology*; D015726:Giant Cells / Q000737:chemistry / Q000502:physiology; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis*; D010014:Osteolysis / Q000209:etiology*; D012516:Osteosarcoma / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D014409:Tumor Necrosis Factor-alpha; D016173:Macrophage Colony-Stimulating Factor