title: Comparison of cytotoxicity of various surfactants tested on normal human fibroblast cultures using the neutral red test, MTT assay and LDH release.
abstract: We used the neutral red test, MTT assay and lactate dehydrogenase (LDH) release to compare the potential cytotoxicity of six surfactants belonging to different classes--three non-ionic surfactants (Triton x100, octylphenoxypolyethoxy alcohol, from Orion; Tween 60, polyoxyethylene (20) sorbitan monostearate, from ICI Speciality Chemicals; Tween 80, polyoxyethylene (20) sorbitan monolaurate, from Labosi), two anionic surfactants (Texapon K1298, sodium lauryl sulphate, from Henkel; Texapon N40, sodium laurylether sulphate, from Henkel) and one cationic surfactant (benzethonium chloride, from Siber Hegner)--on human fibroblast cultures. According to the LC50 (microg ml(-1)), the tested surfactants can be classified in the following order of increasing cytotoxicity: Tween 80 < Texapon N40 < Tween 60 < Texapon K1298 < Triton x100 < benzethonium chloride.
authors.affiliation: Laboratory of Cosmetology and Industrial Pharmacy, Saint Herblain, France.
authors.forename: B
authors.initials: B
authors.lastname: Arechabala
authors.forename: C
authors.initials: C
authors.lastname: Coiffard
authors.forename: P
authors.initials: P
authors.lastname: Rivalland
authors.forename: L J
authors.initials: LJ
authors.lastname: Coiffard
authors.forename: Y
authors.initials: Y
authors.lastname: de Roeck-Holtzhauer
mesh_terms: D001558:Benzethonium / Q000633:toxicity; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009499:Neutral Red / Q000378:metabolism*; D017830:Octoxynol / Q000633:toxicity; D011136:Polysorbates / Q000633:toxicity; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity; D013501:Surface-Active Agents / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism; D018675:Toxicity Tests
chemical_list: D011136:Polysorbates; D013501:Surface-Active Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C075204:Texapon; D001558:Benzethonium; D009499:Neutral Red; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol; D007770:L-Lactate Dehydrogenase