A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export.
In order to identify additional factors required for nuclear export of messenger RNA, a genetic screen was conducted with a yeast mutant deficient in a factor Gle1p, which associates with the nuclear pore complex (NPC). The three genes identified encode phospholipase C and two potential inositol polyphosphate kinases. Together, these constitute a signaling pathway from phosphatidylinositol 4, 5-bisphosphate to inositol hexakisphosphate (IP6). The common downstream effects of mutations in each component were deficiencies in IP6 synthesis and messenger RNA export, indicating a role for IP6 in GLE1 function and messenger RNA export.
York
J D
JD
Department of Pharmacology, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA. yorkj@acpub.duke.edu
Odom
A R
AR
Murphy
R
R
Ives
E B
EB
Wente
S R
SR
D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007295:Inositol Phosphates / Q000378:metabolism; D009154:Mutation; D009685:Nuclear Envelope / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*
D002352:Carrier Proteins; C101408:GLE1 protein, S cerevisiae; D007295:Inositol Phosphates; D028861:Nuclear Pore Complex Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D010833:Phytic Acid; C066454:inositol 4,5-bisphosphate; C120127:IPK1 protein, S cerevisiae; D017853:Phosphotransferases (Alcohol Group Acceptor); C064990:inositol pentakisphosphate kinase; D010738:Type C Phospholipases