title: Exogenous CCK and gastrin stimulate pancreatic exocrine secretion via CCK-A but also via CCK-B/gastrin receptors in the calf.
abstract: A predominance of the pancreatic cholecystokinin (CCK) receptor of the B/gastrin subtype (CCK-B/G) was reported in calves older than 1 month. Specific CCK-A and CCK-B/G receptor antagonists (SR 27897 and PD 135158, respectively) were used to identify the CCK receptor subtype involved in exogenous CCK- and gastrin-induced exocrine pancreatic responses. Conscious calves (2 months old) with catheterized pancreas, jugular vein and duodenum were used; the pancreatic juice was continuously reinfused. CCK (30 pmol kg-1 min-1, 40 min) evoked an increase in pancreatic juice flow and enzyme secretion, while the same dose of gastrin increased enzyme secretion alone. CCK-induced pancreatic secretion was abolished by SR 27897 (15 nmol kg-1 min-1, 55 min) and reduced by PD 135158 (0.15 nmol kg-1 min-1, 55 min). Gastrin-induced enzyme secretion was reduced by PD 135158 (50% to 90%) and to a lesser extent by SR 27897 (50% to 60%). These results demonstrate that CCK and gastrin in the physiological range stimulate pancreatic exocrine secretion in calves and that these effects are partly mediated by CCK-B/G receptors. Although CCK-A receptors are not predominantly expressed, they seem to play a major role in the response of pancreatic exocrine secretion to CCK.
authors.affiliation: INRA, Laboratoire du Jeune Ruminant, 65 rue de Saint-Brieuc, F-35042 Rennes cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Le Dréan
authors.forename: I
authors.initials: I
authors.lastname: Le Huërou-Luron
authors.forename: M
authors.initials: M
authors.lastname: Gestin
authors.forename: C
authors.initials: C
authors.lastname: Desbois
authors.forename: V
authors.initials: V
authors.lastname: Romé
authors.forename: C
authors.initials: C
authors.lastname: Bernard
authors.forename: M
authors.initials: M
authors.lastname: Dufresne
authors.forename: L
authors.initials: L
authors.lastname: Moroder
authors.forename: D
authors.initials: D
authors.lastname: Gully
authors.forename: J A
authors.initials: JA
authors.lastname: Chayvialle
authors.forename: D
authors.initials: D
authors.lastname: Fourmy
authors.forename: P
authors.initials: P
authors.lastname: Guilloteau
mesh_terms: D000818:Animals; D002417:Cattle; D002766:Cholecystokinin / Q000494:pharmacology*; D005755:Gastrins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007210:Indoleacetic Acids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008297:Male; D008536:Meglumine / Q000031:analogs & derivatives / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000187:drug effects*; D013268:Stimulation, Chemical; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D005755:Gastrins; D007210:Indoleacetic Acids; D007211:Indoles; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; D013844:Thiazoles; C065600:PD 135158; C080460:SR 27897; D008536:Meglumine; D002766:Cholecystokinin