[Detection of NV-HB/s DNA and c-myc mRNA in mice inoculated with hepatitis B nucleic acid vaccine--NV-HB/s].
OBJECTIVE
To explore the persistence period of plasmid in mice muscles injected with NV-HB/s and the possible influence on oncogene C-myc.


METHODS
Anti-HBs was detected by ELISA; NV-HB/s was detected by In-situ and Southern-blot hybridization separately; C-myc mRNA was detected by In-situ hybridization.


RESULTS
All mice injected with NV-HB/s were positive of anti-HBs after 1 month of immunization; NV-HB/s was negative when detected by In-situ hybridization; By Southern-blot hybridization, the positive rates of NV-HB/s were decreasing from 100% in 2 months to 25% after 6 months of inoculation.


CONCLUSIONS
NV-HB/s would be disintegrated little by little after injected into mouse muscles; no C-myc activation was observed in 6 months after NV-HB/s inoculation.
Qin
S
S
Viral Hepatitis Research Unit, West China University of Medical Sciences, Chengdu.
Zhao
L
L
Tang
H
H
D000818:Animals; D016259:Genes, myc; D006510:Hepatitis B Antibodies / Q000097:blood; D017325:Hepatitis B Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis*; D019444:Vaccines, DNA / Q000276:immunology*
D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D012333:RNA, Messenger; D019444:Vaccines, DNA