title: Convenient Assay for Settlement Inducing Substances of Barnacles.
abstract: A convenient assay method for estimation of barnacle, Balanus amphitrite, settlement inductive activity was developed. To avoid the inductive effect by comrades and laborious observation requirements, cyprid larvae were put individually into wells of a 96-well plate. The settlement ratio from the experiment without any inducers was quite low; therefore this assay allowed easy estimation of settlement inductive activities. Some known inductive agents, such as serotonin and barnacle extracts, clearly showed inductive activities. This assay method is proven to be suitable for estimation of barnacle settlement-inducing activities of both water-soluble and -insoluble compounds.
authors.affiliation: Marine Biotechnology Institute, Shimizu Laboratories, 1900 Sodeshi, Shimizu, Shizuoka 424, Japan
authors.initials: H
authors.lastname: Kawahara
authors.initials: R
authors.lastname: Tamura
authors.initials: S
authors.lastname: Ajioka
authors.initials: Y
authors.lastname: Shizuri