title: Patterns of adrenoceptor change during liver regeneration of the Wistar Kyoto rat: a binding study.
abstract: BACKGROUND
  Adrenoceptors have been involved in the regulation of hepatocyte proliferation after partial hepatectomy, as well as in primary culture. This report characterizes alpha 1- and beta-adrenoceptor change during the time-course of liver regeneration in adult Wistar Kyoto rats.
  
  
  METHODS
  Saturation binding assays with [3H]prazosin or [3H]dihydroalprenolol (for alpha 1- and beta-adrenoceptors, respectively) were done in liver plasma membranes from 6-month-old rats subjected to 70% hepatectomy followed by hepatic regeneration.
  
  
  RESULTS
  [3H]Prazosin and [3H]dihydroalprenolol binding gave control Bmax values of 101 +/- 10 and 12 +/- 1 fmol/mg protein and Kd of 0.50 +/- 0.10 and 4.1 +/- 0.4 nM for alpha 1- and beta-adrenoceptors, respectively. alpha 1-Adrenoceptor number and Kd increased at 24 and 48 h and returned to control values at 72 and 96 h after surgery, whereas beta-adrenoceptors augmented at 48 and 72 h, with a Kd change at 24 and 48 h posthepatectomy.
  
  
  CONCLUSIONS
  These results suggest that dual control of alpha 1- and beta-adrenoceptor membrane expression could be involved in different steps during hepatocyte proliferation, and that Wistar Kyoto rats have a different adrenoceptor pattern expression from other rat strains.
authors.affiliation: Departamento de Farmacología y Toxicología, Instituto Politécnico Nacional (CINVESTAV-IPN), México, D.F., México.
authors.forename: V
authors.initials: V
authors.lastname: Reyes-Salcido
authors.forename: R
authors.initials: R
authors.lastname: Villalobos-Molina
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004082:Dihydroalprenolol / Q000378:metabolism; D006498:Hepatectomy; D007700:Kinetics; D008115:Liver Regeneration; D008297:Male; D011224:Prazosin / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D011941:Receptors, Adrenergic / Q000378:metabolism*
chemical_list: D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D011941:Receptors, Adrenergic; D004082:Dihydroalprenolol; D011224:Prazosin