A Uve1p-mediated mismatch repair pathway in Schizosaccharomyces pombe.
UV damage endonuclease (Uve1p) from Schizosaccharomyces pombe was initially described as a DNA repair enzyme specific for the repair of UV light-induced photoproducts and proposed as the initial step in an alternative excision repair pathway. Here we present biochemical and genetic evidence demonstrating that Uve1p is also a mismatch repair endonuclease which recognizes and cleaves DNA 5' to the mispaired base in a strand-specific manner. The biochemical properties of the Uve1p-mediated mismatch endonuclease activity are similar to those of the Uve1p-mediated UV photoproduct endonuclease. Mutants lacking Uve1p display a spontaneous mutator phenotype, further confirming the notion that Uve1p plays a role in mismatch repair. These results suggest that Uve1p has a surprisingly broad substrate specificity and may function as a general type of DNA repair protein with the capacity to initiate mismatch repair in certain organisms.
Kaur
B
B
Department of Biochemistry, Graduate Program in Biochemistry and Cell and Developmental Biology, Emory University, School of Medicine, Atlanta, Georgia 30322, USA.
Fraser
J L
JL
Freyer
G A
GA
Davey
S
S
Doetsch
P W
PW
D020137:Base Pair Mismatch; D004260:DNA Repair; D004271:DNA, Fungal / Q000528:radiation effects; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D016296:Mutagenesis; D010641:Phenotype; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D013379:Substrate Specificity; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; D004706:Endodeoxyribonucleases; C105073:uve1 protein, S pombe