Molecular cloning of cDNA for matriptase, a matrix-degrading serine protease with trypsin-like activity.
A major protease from human breast cancer cells was previously detected by gelatin zymography and proposed to play a role in breast cancer invasion and metastasis. To structurally characterize the enzyme, we isolated a cDNA encoding the protease. Analysis of the cDNA reveals three sequence motifs: a carboxyl-terminal region with similarity to the trypsin-like serine proteases, four tandem cysteine-rich repeats homologous to the low density lipoprotein receptor, and two copies of tandem repeats originally found in the complement subcomponents C1r and C1s. By comparison with other serine proteases, the active-site triad was identified as His-484, Asp-539, and Ser-633. The protease contains a characteristic Arg-Val-Val-Gly-Gly motif that may serve as a proteolytic activation site. The bottom of the substrate specificity pocket was identified to be Asp-627 by comparison with other trypsin-like serine proteases. In addition, this protease exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Thus, the protease is a mosaic protein with broad spectrum cleavage activity and two potential regulatory modules. Given its ability to degrade extracellular matrix and its trypsin-like activity, the name matriptase is proposed for the protease.
Lin
C Y
CY
Lombardi Cancer Center, Georgetown University Medical Center, Washington, D. C. 20007, USA.
Anders
J
J
Johnson
M
M
Sang
Q A
QA
Dickson
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D020134:Catalytic Domain; D002918:Chymotrypsin / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D008895:Milk, Human / Q000201:enzymology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000235:genetics*; D014357:Trypsin / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D012697:Serine Endopeptidases; C485595:matriptase; D002918:Chymotrypsin; C485596:ST14 protein, human; D014357:Trypsin