[The effect of blocking insulin-like growth factor-1 (IGF-1)-receptor system on tendon cell proliferation].
The purpose of this study was to find some solutions to the problem of tendon cell proliferation control. Under the condition of in vitro culture, several materials including IGF-1 receptor antibody and mRNA antisense oligonucleotide were added to the culture medium to block the IGF-1-Receptor system. The effect of the material on the tendon cell proliferation was judged by cell count after incubation of 48 hours. The results showed that both IGF-1 Receptor antibody (IGF-1R alpha) and IGF-1 Receptor mRNA antisense oligonucleotide had negative effect on tendon cell proliferation (P < 0.01 and P < 0.05). These findings lead us to think that the above two materials could be used in the experiment of tendon adhesion preventing and living ready-made tendon producing.
Xiang
Z
Z
Department of Orthopedic Surgery, First Univerisity Hospital, West China University of Medical Sciences, Chengdu, P. R. China.
Yang
Z
Z
Zhang
C
C
D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D005333:Fetus; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger / Q000494:pharmacology; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000276:immunology; D013710:Tendons / Q000166:cytology*
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1