[Inter-organ variability in enzymatic hydrolysis of ammonium salts in rats in vitro].
The study evaluated the rate and kinetics of enzymatic hydrolysis of N-(2-benzoyloxyethyl)-alkyl-dimethylammonium bromides, potential easily biodegradable disinfectants of soft character. The products of enzymatic hydrolysis of the substrates under study, catalysed by microsomal esterase, included substituted substrates choline and benzoic acid which, as a hydrolytic product, was essayed by HPLC. The effect of the length of the alkyl chain of the individual homologues on the rate of enzymatic hydrolysis and their affinity to microsomal esterase of the rat liver and lung in vitro was examined. The structural modification (varying length of the aliphatic chain on the ammonium nitrogen of these compounds) was found to significantly influence the kinetics of enzymatic hydrolysis of the esteric bond. From the viewpoint of the rate of enzymatic hydrolysis no significant inter-organ variability was observed: in the liver as well as the lung the dependence of the rate of enzymatic hydrolysis on the length of the aliphatic chain possesses the shape of a falling hyperbole with the maxima for BCH2 > BCH4 > BCH8 = BCH10. The specific activity of both esterases ranges within 0.2-3.5 nmol.min-1.mg-1. From the viewpoint of affinity, a marked inter-organ difference was manifested by 10 times higher affinity of the substrates to the lung microsomal esterase in comparison with the liver one. The effect of the length of the alkyl of the individual homologues on the affinity is of a non-linear character also in this case. In both organs, a certain correlation was found between the rate of enzymatic hydrolysis and affinity to microsomal esterases.
Kissová
E
E
Katedra bunkovej a molekulovej biológie lieciv Farmaceutickej fakulty Univerzity Komenského, Bratislava.
Pauliková
I
I
Helia
O
O
Krenek
P
P
Devínsky
F
F
Lacko
I
I
D000818:Animals; D004950:Esterases / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000201:enzymology; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000644:Quaternary Ammonium Compounds; D004950:Esterases
Medziorgánová variabilita enzýmovej hydrolýzy organických amóniových solí u potkana in vitro.