Ribosomal DNA sequencing as a tool for identification of bacterial pathogens.
Conventional methods for the identification and characterization of clinical isolates of bacterial pathogens sometimes fall short when such isolates exhibit unusual phenotypic profiles. Recent advances in DNA sequencing technology have greatly enhanced the ability of the microbiologist to determine the identity of a bacterial isolate. Given the relative objectivity of DNA sequence information and growing availability of sequence information databases, a significant movement is now afoot to use molecular methods for the identification of clinical pathogens.
Kolbert
C P
CP
Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 200 1st Street SW, Rochester, MN 55905, USA. Kolbert.Christopher@mayo.edu
Persing
D H
DH
D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S