title: Modification of membrane currents in mouse neuroblastoma cells following infection with rabies virus.
abstract: 1. The effect on membrane currents of infection of mouse neuroblastoma NA cells with rabies virus was studied by using the whole-cell patch clamp technique. 2. Three types of membrane currents, namely voltage-dependent Na+ current (I(Na)), delayed rectifier K+ current (I(K-DR)) and inward rectifier K+ current (I(K-IR)) were elicited in uninfected cells. 3. In cells 3 days after infection with the virus, no detectable change was observed in morphology and membrane capacitance, but I(Na) and I(K-IR) were significantly decreased in amplitude without any appreciable difference in the time course of current activation and inactivation. The voltage-dependence of I(Na) activation was significantly shifted in the positive direction along the voltage axis with a decreased slope. I(K-DR) remained almost unaltered after the viral infection. 4. The resting membrane potential, measured with a physiological K+ gradient across the cell membrane, was decreased (depolarized) after the viral infection. The depolarization was associated with the decreased amplitude of I(K-IR). 5. These results suggest that infection of mouse neuroblastoma NA cells with rabies virus causes reduction of functional expression of ion channels responsible for I(Na) and I(K-IR), and provide evidence for possible involvement of the change in membrane properties in the pathogenesis of rabies disease.
authors.affiliation: Department of Veterinary Science, Faculty of Agriculture, Gifu University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Komori
authors.forename: T
authors.initials: T
authors.lastname: Unno
authors.forename: N
authors.initials: N
authors.lastname: Minamoto
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D006224:Cricetinae; D017097:Electric Impedance; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D009447:Neuroblastoma / Q000503:physiopathology* / Q000821:virology*; D015221:Potassium Channels / Q000502:physiology; D011818:Rabies / Q000503:physiopathology*; D011820:Rabies virus; D015222:Sodium Channels / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D015221:Potassium Channels; D015222:Sodium Channels