The effect of daunorubicin and glutaraldehyde treatment on the structure of erythrocyte membrane.
The effect of daunorubicin (DNR) and glutaraldehyde on erythrocyte membrane structure was examined by Electron Spin Resonance spectroscopy. Human erythrocytes were incubated with daunorubicin and then with glutaraldehyde to prevent drug efflux. We have demonstrated that DNR alone caused changes in membrane fluidity mainly in the hydrophobic regions of the lipid bilayer. When DNR-preincubated erythrocytes were treated with glutaraldehyde, the alterations in fluidity were observed in the polar regions as well as in the deeper regions of the cell membrane. The incorporation of drug and glutaraldehyde into human erythrocytes also caused conformational alterations in membrane cytoskeletal proteins and changes in the internal viscosity of the cells. The results suggest that glutaraldehyde in the drug-pretreated erythrocytes may lead to significant perturbations in the organization of the plasma membrane lipids and proteins.
Szwarocka
A
A
Department of Thermobiology, University of Lódź, 90-237, Lódź, Poland.
Jóźwiak
Z
Z
D003630:Daunorubicin / Q000493:pharmacokinetics / Q000494:pharmacology*; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D008301:Maleimides; D008560:Membrane Fluidity / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013113:Spin Labels; D014783:Viscosity
D008301:Maleimides; D013113:Spin Labels; C043592:maleimide; D005976:Glutaral; D003630:Daunorubicin