Serotypes and electrophoretic protein profiles of Pasteurella haemolytica isolated from pneumonic ovine lungs.
Serotypes and SDS-PAGE protein profiles of P. haemolytica isolated from pneumonic ovine lungs were investigated. Of 268 P. haemolytica isolates, 232 (86.6%) were serotypable. A total of 12 serotypes were recognized in 20 different geographic origins of central Turkey. The most common serotype was A2, followed by A7, A1 and T4. Serotypes A13, A14, A16 and T15 could not be detected. In SDS-PAGE, marked differences between major bands of biotype A and T strains were found. In numerical analysis of protein profiles, biotype A and T strains were separated at 58% similarity level. Biotype A isolates produced a cluster at 80% similarity level, and biotype T isolates at 92% similarity level. No single cut off level was able to discriminate between each serotype studied and isolates could not be clustered on the basis of their geographic origins.
Diker
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KS
Department of Microbiology, Faculty of Veterinary Medicine, University of Ankara, Turkey. akan@veterinary.edu.tr
Akan
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Kaya
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D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D015373:Bacterial Typing Techniques / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D016978:Mannheimia haemolytica / Q000737:chemistry / Q000145:classification*; D012766:Pasteurellosis, Pneumonic / Q000382:microbiology*; D012703:Serotyping / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D001426:Bacterial Proteins