title: The gene for an exopolyphosphatase of Pseudomonas aeruginosa.
abstract: In Pseudomonas aeriginosa, a gene, ppx, that encodes exopolyphosphatase [exopoly(P)ase; EC 3.6.1.11] of 506 amino acids (56,419 Da) was found downstream of the gene for polyphosphate kinase, ppk. Since ppx is located in the opposite direction of the ppk gene, they do not constitute an operon. The predicted amino acid sequence of PPX is 41% identical with Escherichia coli PPX. The gene product of ppx (paPPX) was overproduced in E. coli, and its activity was evaluated. Orthophosphate (Pi) is released from polyphosphate [poly(P)], the average chain lengths of which are 79 and 750, respectively. The amount of Pi released matched the amount of poly(P) lost. Thus ppx encodes an enzyme that has exopoly(P)ase activity.
authors.affiliation: Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyake
authors.forename: T
authors.initials: T
authors.lastname: Shiba
authors.forename: A
authors.initials: A
authors.lastname: Kameda
authors.forename: Y
authors.initials: Y
authors.lastname: Ihara
authors.forename: M
authors.initials: M
authors.lastname: Munekata
authors.forename: K
authors.initials: K
authors.lastname: Ishige
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D017766:Acid Anhydride Hydrolases; C020137:exopolyphosphatase