Protection against Helicobacter pylori infection by intestinal immunisation with a 50/52-kDa subunit protein.
A mouse model of Helicobacter pylori infection was used to evaluate the vaccine antigen potential of the citrate synthase homologue protein purified from the H. pylori NCTC 11637 strain. Mice were immunised with the protein by intra-Peyer's patch immunisation. This route gives maximal intestinal immunisation and was used to screen oral vaccine candidate antigens without the added complication of simultaneously testing oral delivery systems. Two weeks post-immunisation mice were infected with Sydney strain H. pylori and 4 weeks after infection the mice were killed and the level of H. pylori infection in the stomach determined. Pre-immunisation with the 50/52-kDa protein led to a 84-91% reduction in H. pylori infection compared to unimmunised controls.
Dunkley
M L
ML
The Australian Institute of Mucosal Immunology, Newcastle, NSW. mdunkley@mail.newcastle.edu.au
Harris
S J
SJ
McCoy
R J
RJ
Musicka
M J
MJ
Eyers
F M
FM
Beagley
L G
LG
Lumley
P J
PJ
Beagley
K W
KW
Clancy
R L
RL
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000276:immunology*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D002950:Citrate (si)-Synthase