title: Heterogeneity of the microglial response in photochemically induced focal ischemia of the rat cerebral cortex.
abstract: This study examined microglial responses after photochemically induced focal ischemia of the rat cortex. Microglial activation exceeded by far the area of the ischemic lesion. Based on morphological criteria and expression of immunomolecules three distinct patterns could be distinguished. (1) In the infarct core and the border zone microglia transformed into phagocytes and removed debris with the aid of hematogeneous macrophages. Exclusively in this area a subpopulation of CD8+ microglia/mnacrophages was present. (2) In secondarily degenerating fibre tracts and nuclei with retrograde neuronal loss, microglia were activated with a delay of days and showed increased expression of complement receptor 3, major histocompatibility complex class II and CD4 molecules, but only low phagocytic activity. (3) In remote ipsilateral cortex devoid of neuronal damage, microglia transiently responded by increased complement receptor 3, but not by major histocompatibility complex class II and CD4 expression. Furthermore, the total number of microglia had increased. This remote response could partly be blocked by dizocilpine maleate, a non-competitive N-methyl-D-aspartate receptor antagonist, implicating a functional role of spreading depression. Taken together, our findings point to a tight and differential regulation of microglial responses in the infarct core, degenerating fibre tracts and remote brain regions without neuronal loss.
authors.affiliation: Department of Neurology and Center for Biological and Medical Research, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schroeter
authors.forename: S
authors.initials: S
authors.lastname: Jander
authors.forename: O W
authors.initials: OW
authors.lastname: Witte
authors.forename: G
authors.initials: G
authors.lastname: Stoll
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002540:Cerebral Cortex / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D002544:Cerebral Infarction / Q000276:immunology / Q000473:pathology*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D007839:Functional Laterality; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000276:immunology / Q000473:pathology*; D017628:Microglia / Q000276:immunology / Q000473:pathology*; D010777:Photochemistry; D051381:Rats; D017208:Rats, Wistar; D012395:Rose Bengal / Q000633:toxicity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D012395:Rose Bengal; D016291:Dizocilpine Maleate