Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys.
The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows the presence of cry1 genes, and amplification with specific primers gave products for cry1 E, cry1 D, cry4 A and cry4 B with sizes different to those expected. Immunoblotting tests showed positive reaction for Cry1 E, Cry3 A, Cry4 A and Cry11 A crystal proteins. The plasmid pattern revealed two large and two small plasmids. Toxicity tests were performed against 14 insects and a slight toxicity was found against Plutella xylotella and Trichoplusia ni.
Ferrandis
M D
MD
Departament de Genètica, Universitat de València, Burjassot, Spain.
Andrew
R
R
Porcar
M
M
Iriarte
J
J
Cosmao-Dumanoir
V
V
Lecadet
M M
MM
Caballero
P
P
Ferré
J
J
D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification* / Q000302:isolation & purification* / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000633:toxicity; D001427:Bacterial Toxins / Q000235:genetics / Q000633:toxicity; D015153:Blotting, Western; D001838:Bolivia; D001517:Coleoptera / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000235:genetics / Q000633:toxicity; D006460:Hemolysin Proteins; D007814:Larva / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D010957:Plasmids / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis