title: The mouse as a model to study adhesion formation following endoscopic surgery: a preliminary report.
abstract: Our aim was to investigate the feasibility of a mouse model to study adhesion formation following endoscopic surgery. Following preliminary studies to establish anaesthesia and pneumoperitoneum pressure, a prospective randomized study was carried out to investigate the effect of CO2 pneumoperitoneum on postoperative adhesions. In group I (control group), the duration of pneumoperitoneum was shorter than 5 min. In groups II, III and IV, pneumoperitoneum was maintained for 60 min without flow, with a continuous low flow (1 ml/min) and a continuous high flow (10 ml/min) through the abdominal cavities of the mice using non-humidified CO2, respectively. Adhesions were scored after 7 days by laparotomy. The total adhesion scores were 0.9 +/- 0.8 (n = 15) in control group, 2.4 +/- 0.8 (n = 15) (P < 0.001 versus control group) in group II with no flow, 2.6 +/- 1.3 (n = 15) (P < 0.001 versus control group) in group III with a continuous low flow and 4.3 +/- 0.9 (n = 15) (P < 0.001 versus control group and P < 0.001 versus group II and III) in group IV with a continuous high flow. In conclusion, the mouse can be used as a model to study adhesion formation following endoscopic surgery. Duration of CO2 pneumoperitoneum is a co-factor in adhesion formation.
authors.affiliation: Centre for Surgical Technologies (CHT), University Hospital Gasthuisberg, K. U. Leuven, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Yesildaglar
authors.forename: J L
authors.initials: JL
authors.lastname: Ordoñez
authors.forename: I
authors.initials: I
authors.lastname: Laermans
authors.forename: P R
authors.initials: PR
authors.lastname: Koninckx
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D003681:Dehydration / Q000150:complications / Q000209:etiology; D004724:Endoscopy; D007327:Insufflation / Q000401:mortality; D051379:Mice; D008815:Mice, Inbred Strains; D010532:Peritoneal Diseases / Q000209:etiology*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects; D011183:Postoperative Complications; D013997:Time Factors; D000267:Tissue Adhesions / Q000209:etiology*
chemical_list: D002245:Carbon Dioxide