Comparison of two kinds of Biolog microplates (GN and ECO) in their ability to distinguish among aquatic microbial communities.
We compared the abilities of Biolog's GN and ECO plates to distinguish among aerobic and heterotrophic bacterial communities in samples from six aquatic environments. The Biolog system is based on interpreting patterns of sole-carbon substrate utilization indicated by color development in a 96-well microtiter plate. Whether of fresh or saltwater origin, bacterial communities utilized > 95% of substrates in both types of plates. Samples from any one environment exhibited similar time courses of average well color development (AWCD) in both GN and ECO plates. Principal component analysis was performed on data sets resulting from combinations of algorithms (AWCD and curve-integration methods) and levels of color development (end-point and set-point approaches). In all cases, the two types of plates demonstrated an equal capacity to discriminate among the heterotrophic expressions of the six microbial communities. Substantial deviation from an anticipated 1:1 correspondence occurred when color development of 25 substrates common to both types of plates was compared. The discrepancies likely are related to the different formulations of low-nutrient media in GN and ECO plates.
Choi
K H
KH
Department of Ocean, Earth, and Atmospheric Sciences, Old Dominion University, Norfolk, VA 23529-0276, USA. kchoi@odu.edu
Dobbs
F C
FC
D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial; D017753:Ecosystem; D005618:Fresh Water; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater; D014871:Water Microbiology