NMR study of a Lewis(X) pentasaccharide derivative: solution structure and interaction with cations.
The structure and conformation of the synthetic pentasaccharide Gal(beta 1-4){Fuc(alpha 1-3)}GlcNAc(beta 1-3)Gal(beta 1-4)Glc-beta OMe of the Lewis(X) family has been determined by NMR spectroscopy in dimethyl sulfoxide and methanol. In these solvents, the binding constants with calcium have been evaluated as 9.5 and 29.6 M-1, respectively. Study of the interaction sites has been achieved through the use of paramagnetic divalent cations and distance triangulation methods. Two regions have been found, the first one in the vicinity of the fucose unit, the second one closer to the lactose part.
Henry
B
B
CEA Saclay, Laboratoire Commun de RMN, DSM/DRECAM/SCM, Gif sur Yvette, France.
Desvaux
H
H
Pristchepa
M
M
Berthault
P
P
Zhang
Y M
YM
Mallet
J M
JM
Esnault
J
J
Sinaÿ
P
P
D002118:Calcium / Q000737:chemistry; D002240:Carbohydrate Sequence; D007477:Ions; D016256:Lewis X Antigen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry
D007477:Ions; D016256:Lewis X Antigen; D009844:Oligosaccharides; D002118:Calcium