title: Subunit D of the vacuolar H+-ATPase of Arabidopsis thaliana.
abstract: A 1034 bp cDNA encoding the full length sequence of subunit D of the vacuolar H+-ATPase was cloned from Arabidopsis thaliana. The open reading frame of the cDNA clone vatpD contains 780 bp and codes for a protein of 29.1 kDa with a pI of 9.52. Structural predictions show similarities to subunit gamma of the F-ATP synthases. Identity between subunit D of the vacuolar H+-ATPase of A. thaliana and subunits D from other eukaryotic organisms is in the range of 57% (Bos taurus) to 48% (Candida albicans). Hybridization of genomic DNA with vatpD indicates the existence of one gene copy of subunit D in A. thaliana. Northern blot hybridization and in situ hybridization showed expression of vatpD in all cell types. The expression of subunit D was not modified by salt stress or abscisic acid treatment in A. thaliana.
authors.affiliation: Department of Physiology and Biochemistry of Plants, Faculty of Biology - W5, University of Bielefeld, 33501, Bielefeld, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Kluge
authors.forename: D
authors.initials: D
authors.lastname: Golldack
authors.forename: K J
authors.initials: KJ
authors.lastname: Dietz
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D006824:Hybridization, Genetic; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D018076:DNA, Complementary; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases