title: A 15-week experimental exposure of pigs to airborne dust with added endotoxin in a continuous flow exposure chamber.
abstract: The purpose of this study was to evaluate the effect of longterm exposure to airborne dust and endotoxin on the respiratory system of pigs. A continuous flow exposure chamber was built for the purpose of exposing pigs to selected airborne contaminants. Pigs (n = 6) were exposed to a combination of a very fine corn/soybean meal (40.6 mg/m3) with added lipopolysaccharide (LPS; 12.4 microg/m3) for 8 h/d over 5 d for 15 wk (75 d of exposure). Control pigs (n = 6) were housed in a room with minimal contamination of these airborne contaminants. Surprisingly, dust in the exposure chamber and the control room was highly contaminated with peptidoglycan. Changes in the lung were monitored by collecting bronchoalveolar lavage (BAL) fluid for cytology at 5 different time points throughout the exposure period. Blood samples were collected at the same time for hematology. A non-specific respiratory inflammatory response was found in exposed and control pigs, as suggested by the increased neutrophils in BAL fluid and the small inflammatory areas in the lung tissue. No macroscopic lung lesions were observed in control or exposed pigs. The findings in the control pigs imply that even low dust concentrations and possibly peptidoglycan contamination can induce cellular changes in the BAL fluid and that a true control pig does not exist. In addition, the exposed pigs developed a mild eosinophilia, indicating an allergic response to the airborne contaminants.
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53704, USA. rika_jolie@groton.pfizer.com
authors.forename: R
authors.initials: R
authors.lastname: Jolie
authors.forename: L
authors.initials: L
authors.lastname: Bäckström
authors.forename: L
authors.initials: L
authors.lastname: Olson
authors.forename: C
authors.initials: C
authors.lastname: Chase
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D016902:Air Pollution, Indoor / Q000032:analysis; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D004391:Dust; D004731:Endotoxins / Q000032:analysis / Q000633:toxicity*; D006799:Housing, Animal; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010457:Peptidoglycan / Q000032:analysis; D013552:Swine; D013997:Time Factors; D014691:Ventilation / Q000379:methods
chemical_list: D000393:Air Pollutants; D004391:Dust; D004731:Endotoxins; D010457:Peptidoglycan