Construction and characterization of Escherichia coli disruptants defective in the yaeM gene.
Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.
Kuzuyama
T
T
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Takahashi
S
S
Seto
H
H
D019747:Aldose-Ketose Isomerases / Q000096:biosynthesis* / Q000235:genetics*; D015139:Blotting, Southern; D002384:Catalysis; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics*; D009154:Mutation / Q000235:genetics / Q000502:physiology*; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D010428:Pentosephosphates / Q000378:metabolism; D010641:Phenotype; D013403:Sugar Phosphates / Q000378:metabolism
C109460:1-deoxylulose 5-phosphate; C114232:2-C-methylerythritol 4-phosphate; D004271:DNA, Fungal; D009097:Multienzyme Complexes; D010428:Pentosephosphates; D013403:Sugar Phosphates; D010088:Oxidoreductases; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; D019747:Aldose-Ketose Isomerases; D004896:Erythritol