title: Dextran restores albumin-inhibited surface activity of pulmonary surfactant extract.
abstract: We examined the effect of dextran (molecular weight 71,000) in counteracting the surfactant inhibitory action of plasma albumin. The surface adsorption time of 0.5 mg/ml modified natural surfactant (MNS; porcine lung extract consisting of phospholipids and hydrophobic surfactant proteins) with 7.5 mg/ml albumin decreased from 681 to 143 s by addition of dextran at a concentration of 10 mg/ml (P < 0.01). The minimum surface tension of 2.0 mg/ml MNS with 30 mg/ml albumin decreased from over 21 mN/m to below 3 mN/m when dextran was added at a concentration of 10 mg/ml (P < 0.01). Surfactant-deficient newborn rabbits given 10 ml/kg of a liquid containing 2.0 mg/ml MNS with 30 mg/ml albumin had a mean tidal volume </=5 ml/kg after 5 min of mechanical ventilation, but, in those animals given the liquid containing 10 mg/ml dextran also, the volume was >13 ml/kg (P < 0.05). Although the underlying mechanism remains to be elucidated, we conclude that dextran restores the albumin-inhibited surface activity of MNS.
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, School of Medicine, Kanazawa University, Kanazawa 920-8641, Japan. kenyam@med.kanazawa-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Tashiro
authors.forename: K
authors.initials: K
authors.lastname: Nishizuka
authors.forename: W M
authors.initials: WM
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Ohmura
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000327:Adsorption; D000818:Animals; D000831:Animals, Newborn; D003911:Dextrans / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D010991:Plethysmography; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D013500:Surface Tension; D013552:Swine
chemical_list: D003911:Dextrans; D011663:Pulmonary Surfactants; D012709:Serum Albumin