title: Rapid detection, by PCR and reverse hybridization, of mutations in the Helicobacter pylori 23S rRNA gene, associated with macrolide resistance.
abstract: A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations, associated with macrolide resistance in Helicobacter pylori. Fifty-seven H. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by PCR-LiPA (detecting mutations A2115-->G, G2141-->A, A2142-->G, A2142-->C, A2143-->G, A2143-->C, and A2143-->T), DNA sequencing, restriction fragment length polymorphism, and/or hybridization to oligonucleotide probes. Results were highly concordant, but PCR-LiPA appears to be more sensitive for the simultaneous detection of multiple mutants.
authors.affiliation: Delft Diagnostic Laboratory, Delft, The Netherlands. L.J.van.Doorn@ddl.nl
authors.forename: L J
authors.initials: LJ
authors.lastname: van Doorn
authors.forename: Y J
authors.initials: YJ
authors.lastname: Debets-Ossenkopp
authors.forename: A
authors.initials: A
authors.lastname: Marais
authors.forename: R
authors.initials: R
authors.lastname: Sanna
authors.forename: F
authors.initials: F
authors.lastname: Mégraud
authors.forename: J G
authors.initials: JG
authors.lastname: Kusters
authors.forename: W G
authors.initials: WG
authors.lastname: Quint
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics; D018942:Macrolides; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D012338:RNA, Ribosomal, 23S