title: Nerve growth factor inducer, 4-methyl catechol, potentiates central sensitization associated with acceleration of spinal glutamate release after mustard oil paw injection in rats.
abstract: 1. In rats, injection of mustard oil (MO) into the paw caused a gradual increase in flinching of the injected paw and this algogenic behavior corresponded with an increase in the CSF-Glu level. 2. The nerve growth factor (NGF) inducer, 4-methyl catechol (4MC), enhanced the frequency of flinching and this effect was dose dependent. In addition, spinal CSF-Glu release was significantly above baseline 10 min after MO injection. In contrast, morphine (MOR) pretreatment completely blocked this behavioral and neurohumoral effect. 3. Anti-NGF paw injection attenuated the algogenic behavior and spinal Glu release otherwise observed after 4MC treatment. 4. The results demonstrated that MO-induced hyperalgesia is associated with increased CSF-Glu release and that this effect is potentiated by a NGF inducer. These data also suggest a possible involvement of NGF in the development of central sensitization after acute peripheral nociceptive stimulation.
authors.affiliation: Department of Anesthesiology-Resuscitology, School of Allied Health Sciences, Yamaguchi University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: O
authors.initials: O
authors.lastname: Nakanishi
authors.forename: N
authors.initials: N
authors.lastname: Funatsu
authors.forename: H
authors.initials: H
authors.lastname: Kameyama
mesh_terms: D000701:Analgesics, Opioid / Q000627:therapeutic use; D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D018698:Glutamic Acid / Q000134:cerebrospinal fluid / Q000187:drug effects* / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D009020:Morphine / Q000627:therapeutic use; D009149:Mustard Plant; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage; D010938:Plant Oils; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D000701:Analgesics, Opioid; D002396:Catechols; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D010936:Plant Extracts; D010938:Plant Oils; C018599:4-methylcatechol; D018698:Glutamic Acid; D009020:Morphine; C027793:mustard oil