[Role of topoisomerase II in the response of mammalian cells to the action of ionizing radiation. I. Change in the mitotic cell cycle of Chinese hamster CHO K1, irradiated with x-rays, after the action of novobiocin at a high concentration].
Flow cytometry was used to study cell cycle recovery in X-irradiated Chinese hamster cells after action of novobiocin, an inhibitor of topoisomerase II. A prolonged treatment with 1 mM novobiocin (20-30 h) of intact cells results in the G2 + M delay. Novobiocin treatment of 5 Gy-irradiated cells results in a slight delay in cell exit from G1 into S phase and in a much longer G2-delay if compared with X-irradiated cells. These data allow to suggest an involvement of topoisomerase II in cell response to ionizing radiation.
Kirillova
T V
TV
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Spivak
I M
IM
D000818:Animals; D016466:CHO Cells / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects*; D006224:Cricetinae; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008938:Mitosis / Q000528:radiation effects*; D009675:Novobiocin / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D009675:Novobiocin; D004250:DNA Topoisomerases, Type II
Rol' topoizomerazy II v otvete kletok mlekopitaiushchikh na deĭstvie ioniziruiushchego izlucheniia. I. Izmenenie mitoticheskogo tsikla kletok kitaĭskogo khomiachka CHO K1, obluchennykh rentogenovskimi luchami, posle deĭstviia novobiotsina v bol'shoĭ kontsentratsii.