Identification of a defect in the intracellular trafficking of a Kell blood group variant.
Blood group polymorphisms have been used as tools to study the architecture of the red blood cell (RBC) membrane. Some blood group variants have reduced antigen expression at the cell surface. Understanding the underlying mechanism for this reduced expression can potentially provide structural information and help to elucidate protein trafficking pathways of membrane proteins. The Kp(a+) phenotype is a variant in the Kell blood group system that is associated with a single amino acid substitution (R281W) in the Kell glycoprotein and serologically associated with a weakened expression of other Kell system antigens by an unknown mechanism. We found by immunoblotting of RBCs that the weakening of Kell antigens in this variant is due to a reduced amount of total Kell glycoprotein at the cell surface rather than to the inaccessibility of the antigens to Kell antibodies. Using a heterologous expression system, we demonstrate that the Kpa mutation causes retention of most of the Kell glycoprotein in a pre-Golgi compartment due to differential processing, thereby suggesting aberrant transport of the Kell protein to the cell surface. Furthermore, we demonstrated that single nucleotide substitutions into the coding region of the common KEL allele, as predicted by the molecular genotyping studies, was sufficient to encode three clinically significant low incidence antigens. We found that two low incidence antigens can be expressed on a single Kell protein, thus showing that the historical failure to detect such a variant is not due to structural constraints in the Kell protein. These studies demonstrate the power of studying the molecular mechanisms of blood group variants for elucidating the intracellular transport pathways of membrane proteins and the requirements for cell surface expression.
Yazdanbakhsh
K
K
New York Blood Center, New York, NY 10021, USA. kyazdan@nybc.org
Lee
S
S
Yu
Q
Q
Reid
M E
ME
D000483:Alleles; D019943:Amino Acid Substitution; D001692:Biological Transport / Q000235:genetics / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007626:Kell Blood-Group System / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017354:Point Mutation; D011110:Polymorphism, Genetic
D007626:Kell Blood-Group System