title: An 8-A projected structure of FhuA, A "ligand-gated" channel of the Escherichia coli outer membrane.
abstract: The structure of FhuA, a siderophore and phage receptor in the outer membrane of Escherichia coli, has been investigated by electron crystallography. Bidimensional crystals of hexahistidine-tagged FhuA protein solubilized in N,N-dimethyldodecylamine-N-oxide were produced after detergent removal with polystyrene beads. Frozen-hydrated crystals (unit cell dimensions of a = 124 A, b = 98 A, gamma = 90 degrees ) exhibited a p22121 plane group symmetry. A projection map at 8 A resolution showed the presence of dimeric ring-like structures with an elliptical shape (48 x 40 A). Each monomer was composed of a ring of densities with a radial width of 8-10 A corresponding to a cylinder of beta sheets. Few densities are present inside the barrel, leaving a central channel approximately 25 A in diameter. A projection map of FhuA at 15 A resolution, which was calculated from negatively stained preparations, demonstrated that most of the central channel was masked by extramembrane domains. This map also revealed an asymmetric distribution of extramembrane domains in FhuA, with large domains located mainly on one side of the molecule. Comparison with density maps derived from recent atomic structure allowed further interpretation of the electron microscopy projection structures with regard to long hydrophilic loops governing the selectivity and opening of the channel.
authors.affiliation: Section de Recherche, Institut Curie, UMR 168 CNRS et, 11 rue Pierre et Marie Curie, Paris, F-75231, France.
authors.forename: O
authors.initials: O
authors.lastname: Lambert
authors.forename: G S
authors.initials: GS
authors.lastname: Moeck
authors.forename: D
authors.initials: D
authors.lastname: Levy
authors.forename: L
authors.initials: L
authors.lastname: Plançon
authors.forename: L
authors.initials: L
authors.lastname: Letellier
authors.forename: J L
authors.initials: JL
authors.lastname: Rigaud
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D005583:Fourier Analysis; D007473:Ion Channels / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011510:Proteolipids / Q000737:chemistry; D011991:Receptors, Virus / Q000737:chemistry* / Q000648:ultrastructure; D013604:T-Phages / Q000648:ultrastructure
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D007473:Ion Channels; D011510:Proteolipids; D011991:Receptors, Virus; C020485:proteoliposomes