High mobility group-like protein in bovine milk stimulates the proliferation of osteoblastic MC3T3-E1 cells.
The active component in bovine milk on the proliferation of osteoblastic MC3T3-E1 cells was purified and identified. Growth-promoting activity was measured by [(3)H]thymidine incorporation on the cell. The molecular weight of the purified protein was 10 kDa. The amino-terminal sequence of this 10-kDa protein was identical to bovine high mobility group protein (HMG) 1. This 10-kDa protein is suggested to be a basic protein and to have an HMG box, a consensus sequence motif among the HMG family. From these results, we named this protein HMG-like protein. HMG is a ubiquitous nonhistone component of chromatin and considered to be implicated in DNA replication. We found this protein in milk, and it showed a growth-promoting activity. We propose the possibility that HMG-like protein existed in milk and plays an important role for neonate in bone formation by activating osteoblasts.
Nutritional Science Laboratory, Snow Brand Milk Products Co., Ltd., 1-1-2 Minamidai, Saitama, Kawagoe, 350-1165, Japan.
J
J
Yamamura
Y
Y
Takada
M
M
Goto
M
M
Kumegawa
S
S
Aoe
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D016384:Consensus Sequence / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D008894:Milk Proteins / Q000737:chemistry* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D016415:Sequence Alignment; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured; D000067816:Whey Proteins
D006609:High Mobility Group Proteins; D008894:Milk Proteins; D000067816:Whey Proteins; D004247:DNA