Photoinduced cross-linking of RNA by cis-Rh(phen)2Cl2+ and cis-Rh(phen)(phi)Cl2+: a new family of light activatable nucleic acid cross-linking agents.
The metal complexes, cis-Rh(phen)2Cl2+ and its more hydrophobic analog cis-Rh(phen)(phi)Cl2+, have been shown to photocross-link the 120-base phi29-encoded pRNA. Primer extension on the cis-Rh(phen)(phi)Cl2(+)-photocross-linked RNA revealed that guanines are responsible for the interstrand cross-links.
Mohammad
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Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Chen
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C
Guo
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Morrison
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H
D017103:Bacillus Phages / Q000235:genetics; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry; D008027:Light; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D010777:Photochemistry; D012367:RNA, Viral / Q000737:chemistry*
C120940:(1,10-phenanthroline)(9,10-phenanthrenequinonediimine)rhodamine(III); D003432:Cross-Linking Reagents; D009942:Organometallic Compounds; D010616:Phenanthrenes; D010618:Phenanthrolines; D012367:RNA, Viral; C120941:bis(1,10-phenanthroline)rhodium(III); D006147:Guanine