Elevated plasma osmotic concentration stimulates water absorption response in a toad.
The water-seeking behavior (WR) of toads (Bufo viridis) was investigated. Fully hydrated toads that are allowed free choice of wet or dry filter paper voluntarily and spontaneously select to sit on water-soaked paper at a regular frequency during trials. Dehydration of bladder-emptied toads by 14% elicits WR in all animals. Injection of aldosterone or angiotensin-I reduced the dehydration threshold to 7% weight loss. WR frequency increased when plasma osmolality was elevated by injection of NaCl or other solutes (both ionic and non-ionic). Only urea, to which cell membranes are highly permeable, was the exception that did not produce this response. The increase in WR frequency induced by elevated plasma osmolality was augmented by injection of aldosterone or angiotensin-I. In vivo water uptake, measured in a water bath, was increased by an NaCl or oxytocin injection, but not by aldosterone. It is concluded that elevated plasma osmolality induces an increase in WR frequency that is separate and prior to the water uptake process. Different hormones are involved in each step.
Department of Biology, Technion, Israel Institute of Technology, Haifa, Israel. jhoffman@tx.technion.ac.il
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D000450:Aldosterone / Q000494:pharmacology; D000803:Angiotensin I / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D002025:Bufonidae / Q000097:blood / Q000502:physiology*; D003681:Dehydration / Q000097:blood / Q000188:drug therapy / Q000503:physiopathology; D005260:Female; D008297:Male; D009994:Osmolar Concentration; D009995:Osmosis / Q000502:physiology; D010121:Oxytocin / Q000494:pharmacology; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects; D014867:Water / Q000378:metabolism*
D014867:Water; D012965:Sodium Chloride; D000450:Aldosterone; D010121:Oxytocin; D014508:Urea; D000803:Angiotensin I