Effects of anabolic steroids on diaphragm impairment induced by methylprednisolone in emphysematous hamsters.
This study was designed to investigate whether the administration of the anabolic steroid nandrolone decanoate is able to antagonize the loss in diaphragm function induced by long-term administration of a low-dose of methylprednisolone in emphysematous hamsters. Normal and emphysematous male hamsters were randomized to receive either saline or methylprednisolone 0.2 mg x kg(-1) x day(-1) for 9 months, with or without nandrolone decanoate 1 mg x kg(-1) x week(-1) i.m. during the final 3 months. Diaphragm contractile properties and myosin heavy chain composition were determined. Compared to control hamsters, the force generating capacity of isolated diaphragm strips decreased by approximately 12% in the emphysema group and by approximately 22% in the emphysema plus methylprednisolone group. Addition of nandrolone decanoate to the emphysema plus methylprednisolone hamsters significantly improved force generation. The atrophy of type IIa and IIx diaphragm fibres in the emphysema plus methylprednisolone group was completely reversed to the level of control hamsters by the addition of nandrolone decanoate. In conclusion, nandrolone decanoate in part reversed the loss in diaphragm force-generating capacity in emphysematous hamsters treated with methylprednisolone, and reversed type IIa and IIx fibre atrophy completely.
Dept of Pulmonary Diseases, University Hospital Nijmegen, The Netherlands.
R H
RH
van Balkom
P N
PN
Dekhuijzen
H F
HF
van der Heijden
H T
HT
Folgering
J A
JA
Fransen
C L
CL
van Herwaarden
D045930:Anabolic Agents / Q000627:therapeutic use*; D000818:Animals; D006224:Cricetinae; D003964:Diaphragm / Q000187:drug effects* / Q000503:physiopathology; D005938:Glucocorticoids / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D008775:Methylprednisolone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D018995:Myosin Heavy Chains / Q000378:metabolism; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000077603:Nandrolone Decanoate; D010196:Pancreatic Elastase; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000503:physiopathology*; D011897:Random Allocation
D045930:Anabolic Agents; D005938:Glucocorticoids; D009277:Nandrolone; D010196:Pancreatic Elastase; D018995:Myosin Heavy Chains; D000077603:Nandrolone Decanoate; D008775:Methylprednisolone