Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse.
The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 x 10(9) or 5 x 10(9) plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 x 10(9) plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.
Wood
M
M
Introgen Therapeutics Inc., Houston, Texas 77054, USA.
Perrotte
P
P
Onishi
E
E
Harper
M E
ME
Dinney
C
C
Pagliaro
L
L
Wilson
D R
DR
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008156:Luciferases / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000201:enzymology
D008156:Luciferases