In situ hybridization in paracoccidioidomycosis.
In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.
Institute of Tropical Medicine and School of Dentistry, S. Paulo University, SP, Brazil. thalesbr@usp.br
T
T
De Brito
G S
GS
Sandhu
B C
BC
Kline
R A
RA
Aleff
M P
MP
Sandoval
R T
RT
Santos
A A
AA
Brandão
C S
CS
Lacaz
D000328:Adult; D000818:Animals; D006168:Guinea Pigs; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008875:Middle Aged; D010228:Paracoccidioides / Q000235:genetics*; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000473:pathology