Selective coupling of mouse brain metabotropic sigma receptor with recombinant Gi1.
Various sigma (sigma) ligands including (+)-pentazocine stimulated [35S]GTPgammaS binding in synaptic membranes from the mouse cerebellum. The (+)-pentazocine-stimulated [35S]GTPgammaS binding was blocked by the treatment of membranes with pertussis toxin (PTX), but completely recovered by the reconstitution of PTX-treated membranes with recombinant Gi1, but not with GoA. These findings suggest that metabotropic sigma receptors are selectively coupled to Gi1 protein.
Department of Molecular Pharmacology and Neuroscience, Nagasaki University School of Pharmaceutical Sciences, Japan.
S
S
Tokuyama
K
K
Hirata
A
A
Yoshida
J
J
Maruo
K
K
Matsuno
S
S
Mita
H
H
Ueda
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D001923:Brain Chemistry; D019281:Dimerization; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010423:Pentazocine / Q000494:pharmacology; D037342:Pertussis Toxin; D017480:Receptors, sigma / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D013462:Sulfur Radioisotopes / Q000378:metabolism; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D017480:Receptors, sigma; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D010566:Virulence Factors, Bordetella; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D037342:Pertussis Toxin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D010423:Pentazocine