Molecular biological characterization of phosphodiesterase 3A from human corpus cavernosum.
The hydrolysis of the second messenger cyclic AMP (cAMP) by phosphodiesterase 3 (PDE3) is known to play an important regulatory role in the context of relaxation of cavernous smooth muscle of the penis. Thus, we investigated the PDE3A isoform from penile cavernous tissues of male patients with and without symptoms of erectile dysfunction at the molecular biological level. As revealed by reverse transcriptase polymerase chain reaction, of all tissues of the urogenital tract analyzed the expression of the PDE3A gene was highest in the corpus cavernosum. However, significant differences in the levels of gene expression were not found between the two subgroups of patients. Also, the determined nucleotide sequences of the cloned penile PDE3A cDNAs of all patients were absolutely identical. Surprisingly, some deviations could be detected in the cDNA sequences of PDE3A from human myocard and platelets. The data obtained indicate that neither the expression levels nor the sequence deviations of PDE3A are the main reasons for erectile dysfunction in men.
Küthe
A
A
Hannover Medical School, Department of Urology, Germany. akuethe@gmx.de
Eckel
H
H
Stief
C G
CG
Uckert
S
S
Forssmann
W G
WG
Jonas
U
U
Mägert
H J
HJ
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D007172:Erectile Dysfunction / Q000201:enzymology / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology* / Q000502:physiology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity / Q000235:genetics; D010413:Penis / Q000201:enzymology* / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517287:PDE3A protein, human