Estrogen regulates gonadotropin-releasing hormone in the nervus terminalis of Xenopus laevis.
The nervus terminalis or terminal nerve (TN) is a neuronal plexus found in the nasal cavity and rostral forebrain of most vertebrates. The hormone gonadotropin-releasing hormone (GnRH) is found in a population of TN neurons as well as hypothalamic neurons which regulate pituitary secretion of the gonadotropins. The GnRH-containing neurons of the TN appear to represent a rostral continuation of the hypothalamic population since they both originate from the olfactory placode and are frequently anatomically continuous. Previous studies have shown that the hypothalamic GnRH neurons are regulated by circulating estrogen levels. Ovariectomy decreases while estrogen administration increases GnRH content in these neurons. It is not known whether the GnRH-containing TN neurons are also regulated in a similar manner. This study demonstrates that ovariectomy and estrogen readministration alters GnRH-immunoreactive (ir) levels in the TN of female Xenopus laevis in a manner similar to that seen in the hypothalamus. One week after ovariectomy, the density of TN GnRH-ir fibers in the olfactory bulb region (one site of TN termination) is significantly decreased. In contrast, a significant increase in GnRH-ir TN fiber density is observed following estrogen readministration to ovariectomized frogs. These findings demonstrate that estrogen regulates GnRH metabolism in neurons of the TN.
Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73190, USA.
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Wirsig-Wiechmann
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CE
Lee
D000818:Animals; D003720:Densitometry; D004967:Estrogens / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007150:Immunohistochemistry; D009411:Nerve Endings / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism; D014982:Xenopus laevis
D004967:Estrogens; D007987:Gonadotropin-Releasing Hormone