Identification and characterization of mRNAs differentially expressed in thyroid cells stimulated by a mitogenic treatment.
The aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A first study has allowed to identify three new genes. Further search will use the differential display and gene arrays methodology. The role of the identified proteins coded by the genes is studied in vitro by the search of partner proteins by the double hybrid method and in vivo by mice gene knockout technology.
Pirson
I
I
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
Behrends
J
J
Savonet
V
V
Goffard
J C
JC
Dumont
J E
JE
Schurmans
S
S
Maenhaut
C
C
D000818:Animals; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008934:Mitogens / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger; D013961:Thyroid Gland / Q000166:cytology
D008934:Mitogens; D011506:Proteins; D012333:RNA, Messenger