Species-specific primers of chitin synthase 1 gene for the differentiation of the Trichophyton mentagrophytes complex.
Species-specific primers were designed from nucleotide sequences of the chitin synthase 1 gene (CHS1) of Arthroderma benhamiae, A. simii and A. vanbreuseghemii. The A. benhamiae-specific primer amplified a 560-bp fragment from A. benhamiae but not from A. simii and A. vanbreuseghemii. The A. simii-specific primers amplified a 470-bp fragment from A. simii but not from A. benhamiae and A. vanbreuseghemii. The A. vanbreuseghemii-specific primers amplified a 360-bp fragment from A. vanbreuseghemii but not from A. benhamiae and A. simii. With these species-specific primers of CHS1 genes, 10 clinical isolates from humans and animals were examined by polymerase chain reaction analyses. These isolates proved to be identical to A. vanbreuseghemii.
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
R
R
Kano
Y
Y
Nakamura
T
T
Watari
S
S
Watanabe
H
H
Takahashi
H
H
Tsujimoto
A
A
Hasegawa
D000818:Animals; D003883:Arthrodermataceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D002687:Chitin Synthase / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D003881:Dermatomycoses / Q000382:microbiology; D005800:Genes, Fungal; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014249:Trichophyton / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal; D002687:Chitin Synthase