Egg-hatching inhibition in mice immunized with recombinant Schistosoma bovis 28 kDa glutathione S-transferase.
The capacity of a recombinant glutathione S-transferase from Schistosoma bovis (rSb 28GST) to protect BALB/c mice against homologous and heterologous infections with, respectively, S. bovis or Schistosoma mansoni has been studied. Two injections of the rSb 28GST and an intravenous boost resulted in a marked specific IgG response on the day of experimental challenge with S. bovis or S. mansoni cercariae. Immunization of BALB/c mice led to a reduction in egg maturation and egg viability after infection with S. bovis or S. mansoni. Adult worm recoveries after an S. bovis challenge infection and tissue egg densities (intestine and liver) in S. mansoni challenge infection were also reduced in the immunized groups, but these differences were not statistically significant. No association between in vitro inhibition of GST enzymatic activity induced by immunized mouse sera and worm burden reduction was recorded. The analysis of the immune response, on the day of perfusion, showed the production of immunoglobulin (Ig)G1, IgG2a and IgG2b specific antibodies and the production of interleukin (IL)-4 and IL-5 by spleen cells after rSb 28GST stimulation. These data suggest that rSb 28GST immunization induces a moderate effect upon egg maturation and egg hatching, suggesting the involvement of similar mechanisms of action and common, but not exclusive, targets during S. bovis and S. mansoni infections. As a consequence, immunization with rSb 28GST may prove useful in affecting the pathology and transmission of African schistosomes.
Centre d'Immunologie et de Biologie Parasitaire, Unité INSERM U-167, IFR 17, Institut Pasteur de Lille, 1 rue du Pr Calmette, 59019 LILLE cedex, France.
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Da Costa
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Gaubert
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Lafitte
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Fontaine
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Capron
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Grzych
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005982:Glutathione Transferase / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D012547:Schistosoma / Q000276:immunology* / Q000502:physiology*; D012550:Schistosoma mansoni / Q000502:physiology; D012552:Schistosomiasis / Q000469:parasitology / Q000517:prevention & control*; D012555:Schistosomiasis mansoni / Q000469:parasitology / Q000517:prevention & control
D000909:Antibodies, Helminth; D016207:Cytokines; D011994:Recombinant Proteins; D005982:Glutathione Transferase