Expression of NADPH-diaphorase and colocalization with Fos in the brain neurons of the rat following visceral noxious stimulation.
We used double staining immunocytochemical techniques to determine whether nitric oxide (NO) and Fos immunoreactivity induced by noxious visceral stimulation were colocalized in the neurons of the supraspinal areas. We observed a considerable increase in Fos-positive neurons in many brain areas after noxious stimulation but only 15% of the Fos-positive neurons colocalized Nicotinamide Adenine Dinucleotide Phosphate Diaphorase (NADPH-d). The NADPH-d positive cells showed perikarya and cytoplasmic processes laying next to or more frequently apposed to Fos-positive neurons. This anatomical finding supported the hypothesis that also at supraspinal level NO is released near the neurons specifically activated and diffuses through the source cells to act on adjacent neurons playing a role in the central processing of pain transmission and modulation.
Rodella
L
L
Department of Biomedical Sciences and Biotechnology, Division of Human Anatomy, University of Brescia, Via Valsabbina 19, 25124 Brescia, Italy.
Rezzani
R
R
Agostini
C
C
Bianchi
R
R
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D010146:Pain / Q000378:metabolism / Q000503:physiopathology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D014018:Tissue Distribution / Q000502:physiology; D014781:Viscera / Q000503:physiopathology*
D016760:Proto-Oncogene Proteins c-fos; D009252:NADPH Dehydrogenase