Atrial natriuretic-like peptide and its prohormone within metasequoia.
Metasequoia glyptostroboides was one of the dominant conifers in North America, Asia, and Europe for more than 100 million years since the late Cretaceous Albian Age, but Quaternary glaciations drove the Metasequoia population to apparent extinction. A small pocket of Metasequoia, however, was found in central China in the 1940s representing the only surviving population of this "living fossil" species. Atrial natriuretic peptide, a 28-amino-acid peptide hormone that causes sodium and water excretion in animals, has been found to be part of the first peptide hormonal system in lower plants. The existence of this hormonal system has never been examined within trees of any genus. High-performance gel permeation chromatography of the leaves and stems (i.e., branches) of Metasequoia followed by atrial natriuretic peptide radioimmunoassay revealed an ANP-like peptide and its prohormone (i.e., approximately 13,000 mol wt) were present in both leaves and stems of this conifer. The elution profile of ANP-like peptide in stems of Metasequoia had a shoulder to the left of where pure synthetic ANP elutes suggesting the possibility of a slightly larger peptide eluting within this shoulder secondary to alternate processing of the ANP-like prohormone and similar to what occurs with the kidney of animals. The elution profile of ANP-like peptide in the leaves of Metasequoia revealed two peaks; one where ANP elutes and a second peak suggesting a smaller peptide that has been metabolically processed. The presence of the ANP-like prohormone strongly suggests that ANP-like gene expression is occurring in both leaves and stems of Metasequoia since this prohormone is the gene product of this hormonal system. The presence of the ANP-like hormonal system in trees implies that this hormonal system may have been present early in land plant evolution to allow trees to reach heights of greater than 30 feet where a water flow-enhancing substance is absolutely necessary for water flow to occur to these heights.
Yang
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Nanjing Institute of Geology and Paleontology, Chinese Academy of Sciences, Nanjing, 210008 PR China.
Gower
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WR
Li
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Chen
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Vesely
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D009320:Atrial Natriuretic Factor / Q000032:analysis*; D002681:China; D002850:Chromatography, Gel; D019143:Evolution, Molecular; D010937:Plant Growth Regulators / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011498:Protein Precursors / Q000032:analysis
D010937:Plant Growth Regulators; D010940:Plant Proteins; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor