Comparison of a commercial test for serotyping heat-stable antigens of Campylobacter jejuni with genotyping by pulsed-field gel electrophoresis.
A new commercial serotyping set based on heat-stable Penner's antigens was compared with pulsed-field gel electrophoresis (PFGE) with SmaI and SacII restriction endonucleases. Among 50 isolates of Campylobacter jejuni from Finnish patients, which represented predominant PFGE patterns selected from isolates from sporadic cases and isolates associated with small outbreaks, 11 different serotypes were demonstrated from 43 typable isolates. Several PFGE patterns could be found within one serotype; on the other hand, several serotypes could be demonstrated within one PFGE type. Most isolates originated from sporadic cases; however, some isolates were epidemiologically associated and showed identical serotypes and PFGE patterns. Although the new serotyping set would have been useful in the few epidemic cases studied, several isolates (14%) representing the major PFGE patterns remained untypable or gave weakly positive agglutination reactions only suggesting a plausible serotype (18%). This might restrict the use of the novel serotyping set, at least in Finland.
Department of Bacteriology and Immunology, The Haartman Institute, University of Helsinki and Helsinki University Central Hospital Diagnostics.
Hilpi
H
Rautelin
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Marja-Liisa
ML
HäNNINEN
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood* / Q000235:genetics; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000276:immunology; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005387:Finland / Q000453:epidemiology; D005838:Genotype; D006386:Hemagglutination Tests; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012703:Serotyping; D011795:Surveys and Questionnaires
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; C025651:CCCGGG-specific type II deoxyribonucleases; C035337:CCGCGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific