Structure and reaction mechanisms of multifunctional mitochondrial cytochrome bc1 complex.
The cytochrome bc1 complex from bovine heart mitochondria is a multi-functional enzyme complex. In addition to electron and proton transfer activity, the complex also processes an activatable peptidase activity and a superoxide generating activity. The crystal structure of the complex exists as a closely interacting functional dimer. There are 13 transmembrane helices in each monomer, eight of which belong to cytochrome b, and five of which belong to cytochrome c1, Rieske iron-sulfur protein (ISP), subunits 7, 10 and 11, one each. The distances of 21 A between bL heme and bH heme and of 27 A between bL heme and the iron-sulfur cluster (FeS), accommodate well the observed fast electron transfers between the involved redox centers. However, the distance of 31 A between heme c1 and FeS, makes it difficult to explain the high electron transfer rate between them. 3D structural analyses of the bc1 complexes co-crystallized with the Qu site inhibitors suggest that the extramembrane domain of the ISP may undergo substantial movement during the catalytic cycle of the complex. This suggestion is further supported by the decreased in the cytochrome bc1 complex activity and the increased in activation energy for mutants with increased rigidity in the neck region of ISP.
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Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078-3035, USA. cayuq@okway.okstate.edu
Zhang
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Deng
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Tian
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Xia
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Kim
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Deisenhofer
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Yu
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D000818:Animals; D002417:Cattle; D003573:Cytochrome b Group / Q000737:chemistry / Q000378:metabolism; D003575:Cytochromes c1 / Q000737:chemistry / Q000378:metabolism; D019281:Dimerization; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism; D046911:Macromolecular Substances; D008929:Mitochondria, Heart / Q000201:enzymology*; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D003573:Cytochrome b Group; D007506:Iron-Sulfur Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; C037989:Rieske iron-sulfur protein; D003575:Cytochromes c1; D014450:Electron Transport Complex III