Mutated cytochrome b as a determinant of a new monoclonal antibody (H8.98) on renal carcinoma cell lines recognized by a Vgamma3Vdelta1(+) T-cell clone.
We generated a monoclonal antibody (MAb), H8.98, that recognizes an antigen shared by 50% of examined renal carcinoma cell (RCC) lines and is susceptible to lysis by a Vgamma3Vdelta1(+) T-cell clone derived from RCC tumor-infiltrating lymphocytes. H8.98 inhibited Vgamma3Vdelta1(+ )T-cell clone-mediated lysis of RCC lines. It did not stain normal kidney lines, melanomas, fibroblasts, Burkitt's lymphoma or Epstein-Barr virus-transformed B-cell lines but it did stain 2 of 4 tested breast cancer lines. Through screening of a renal carcinoma cDNA library using H8.98, we isolated a cDNA clone which, upon sequencing, was found to be cytochrome b with 2 point mutations.
Earle A. Chiles Research Institute, Providence Portland Medical Center, Portland, OR, USA.
A
A
Choudhary
R A
RA
Kurt
F
F
Goret
A
A
Moreau
E
E
Diéz
W J
WJ
Urba
F
F
Jotereau
C
C
Pourcel
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D019556:COS Cells; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000276:immunology*; D003573:Cytochrome b Group / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D003573:Cytochrome b Group