Comparative evaluation of procedures with a Baxter CS-3000 cell separator for collecting peripheral blood cells from children.
The safety and efficacy of apheresis using a newly developed procedure with a small volume separation container holder (SVSCH, Baxter) for the collection of PBSC from children with cancer were retrospectively compared with our historical experience with other procedures using different equipment and the CS-3000 plus cell separator. The procedures tested included the application of (a) specialized collection protocol 4 using the combination of an SVSCH and a small volume collection chamber (SVCC) (group A: 6 collections in 4 patients), (b) standard lymphocyte collection protocol 3 with GRANULO separation (G) and A-35 collection chambers (group B: 9 collections in 5 patients), and (c) modified collection procedure 1-120 with G and SVCC (group C: 7 collections in 3 patients). Although the retrospective nature of this study and the differences in the cohorts tested prevent a reliable analysis, the percent decrease in the peripheral platelet count after collection tended to be minimum in group A (11% +/- 5% versus 23% +/- 4% [B] or 17% +/- 4% [C]). Moreover, the largest number of CD34+ cells was collected in group A (8.7 +/- 9.4 x 10(5)/100 ml processed blood) compared with groups B (5.5 +/- 7.5 x 10(5)/100 ml processed blood) and C (4.0 +/- 2.8 x 10(5)/100 ml processed blood). These data suggest that a procedure incorporating SVSCH can be safely and effectively applied to small children and enables the selective collection of PBSC with less contamination by platelets, which is useful for subsequent cell processing.
Makimoto
A
A
Department of Pediatrics, University of Tokushima, Kuramoto, Japan.
Kawano
Y
Y
Abe
T
T
Okamoto
Y
Y
Sato
J
J
Nakagawa
R
R
Watanabe
H
H
Watanabe
T
T
Kuroda
Y
Y
Sweet
L
L
Takaue
Y
Y
D000293:Adolescent; D018952:Antigens, CD34 / Q000276:immunology; D001781:Blood Component Removal / Q000009:adverse effects / Q000379:methods*; D001810:Blood Volume; D002469:Cell Separation / Q000295:instrumentation* / Q000592:standards; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005260:Female; D006412:Hematopoietic Stem Cells; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D010976:Platelet Count; D013234:Stem Cells
D018952:Antigens, CD34