[Utilization of Bichrolatex albicans test for the rapid identification of Candida albicans in blood culture bottles in fungemia: preliminary results from a multicenter study].
In patients with fungemia, reliable and rapid identification of the causative organism has a large impact on treatment decisions. The Bichrolatex albicans test (Fumouze, Levallois-Perret, France) allows rapid identification of Candida albicans in colonies. Its usefulness for identifying C. albicans in liquid blood culture media was evaluated using a previously published protocol. Ninety-seven blood culture bottles from 86 fungemia episodes in 13 study centers were studied. C. albicans contributed 52% of the fungemia episodes, whereas C. glabata and C. tropicalis contributed 15% and 11.5%, respectively. The 48 bottles containing a yeast other than C. albicans were all negative by the Bichrolatex albicans test. Of the remaining 48 bottles, which were positive for C. albicans, 33 produced marked to moderate agglutination and 15 were negative. In one case of mixed fungemia (C. albicans plus C. glabata), weak agglutination was seen. Sensitivity of the test was 69%, negative predictive value was 76%, specificity was 100%, and positive predictive value was 100%. Because of its limited sensitivity, the Bichrolatex albicans test cannot be recommended as a confirmation test for C. albicans fungemia. In contrast, the specificity and positive predictive value of the test are excellent.
Microbiologie, Hôpital Foch, Suresnes, France.
P
P
Cahen
F
F
Laurent
P
P
Honderlick
F
F
Saheb
D001769:Blood; D002175:Candida / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis*; D003470:Culture Media; D016469:Fungemia / Q000175:diagnosis*; D006801:Humans; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003470:Culture Media; D011933:Reagent Kits, Diagnostic
Utilisation du réactif Bichrolatex albicans pour l'identification rapide de Candida albicans dans les flacons d'hémoculture au cours des fongémies: résultats préliminaires d'une étude multicentrique.