Growth characteristics of T-cell tropic HIV-1 vpu gene mutants in human peripheral blood mononuclear cells.
A mutant designated NL-E65, which lacks the expression of entire vpu gene, was constructed from T-cell tropic wild-type (wt) human immunodeficiency virus type 1 (HIV-1) clone and monitored for its replication property in human cells, along with a mutant NL-Ss which expresses a C-terminal truncated Vpu. The mutant NL-Ss could grow in two cell lines and in all peripheral blood mononuclear cell (PBMC) preparations to some extent, with kinetics similar to those of wt virus. Likewise, the mutant NL-E65 exhibited a replication property typical to the vpu mutant in the two cell lines and in all PBMC cultures, growing at a low level. Along with the results previously reported, these data indicate that HIV-1 Vpu is dispensable for virus replication in any of the types of cells so far tested.
Department of Virology, University of Tokushima School of Medicine, Japan.
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S
Iida
T
T
Fukumori
Y
Y
Oshima
A H
AH
Koyama
A
A
Adachi
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D002478:Cells, Cultured; D015497:HIV-1 / Q000502:physiology*; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009154:Mutation; D013601:T-Lymphocytes / Q000821:virology; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics; D000086082:Viroporin Proteins
D054298:Human Immunodeficiency Virus Proteins; D054334:Viral Regulatory and Accessory Proteins; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins