Role of polysialic acid on outgrowth of rat olfactory receptor neurons.
Towards elucidating the role of polysialic acid (PSA) in developing olfactory neuron of the rat, we injected neuraminidase (endo-N) into the olfactory nerve pathway under whole embryo culture, then employed immunohistochemistry to (i) detect expression of highly sialylated neural cell adhesion molecules (NCAM-H) and (ii) identify olfactory neurons via anti-microtubule-associated protein 1B (MAP1B) antibody. Olfactory axonal outgrowth from basal lamina occurred at the 31-somite stage and reached the olfactory bulb primordium at the 42-somite stage, being coincident with the timing and expression of NCAM-H immunoreactivity. Enzymatic removal of PSA by endo-N remarkably affected developmental processes of axonal outgrowth, extension, and pathfinding, i.e. individual axons appeared to have become stuck in the mesenchyme. Results indicate that PSA is critically involved with anti-adhesion cues associated with individual axonal growth during olfactory system development.
Department of Physiology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582, Japan. kaoriaok@mc.med.keio.ac.jp
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Aoki
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Nakahara
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Yamada
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Eto
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism; D018034:Olfactory Receptor Neurons / Q000166:cytology* / Q000196:embryology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012794:Sialic Acids / Q000502:physiology*
D008869:Microtubule-Associated Proteins; D019006:Neural Cell Adhesion Molecules; D012794:Sialic Acids; C085284:microtubule-associated protein 1B; C021319:polysialic acid