HLA-B27 subtypes and HLA class II alleles in Japanese patients with anterior uveitis.
OBJECTIVE
Some patients with anterior uveitis (AU) have ankylosing spondylitis (AS) and are HLA-B27 class I-positive. The purpose of this study was to investigate whether there are differences in HLA at the allele level among each group of patients with AU.


METHODS
Seventy-three patients with AU were studied. They were classified into three groups: 31 with AS-associated AU, 14 with HLA-B27-associated AU, and 28 with idiopathic AU. Three control groups without AU were used: 138 random subjects, 33 HLA-B27-positive healthy subjects, and 19 HLA-B27-positive patients with AS. DRB1 and DQB1 genotyping was performed using polymerase chain reaction (PCR)-single-strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism. HLA-B27 subtype was determined by PCR-SSCP.


RESULTS
There was no difference in the frequency of any class I antigen except HLA-B27 among the patients studied. The frequencies of HLA-DR12 in AS-associated AU and HLA-DR1 in HLA-B27-associated AU showed an increase. In HLA-B27-associated AU, DRB1*0101 and DQB1*0501 were increased compared with HLA-B27-positive control subjects. When HLA-B27 subtype distribution was compared among the groups, the proportion of B*2704 was significantly lower in HLA-B27-associated AU (P = 0.037), however, such a difference was not present in AS-associated groups.


CONCLUSIONS
These results indicated that B*2704 seemed to be less susceptible to AU compared with B*2705 in Japanese subjects. The increase of HLA-DR12 and HLA-DR1 in AU may be caused by linkage disequilibrium with B*2704 and B*2705, respectively.
Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan.
Y
Y
Konno
J
J
Numaga
N
N
Tsuchiya
A
A
Ogawa
S M
SM
Islam
M
M
Mochizuki
H
H
Mitsui
H
H
Oda
H
H
Maeda
D000328:Adult; D000483:Alleles; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D013167:Spondylitis, Ankylosing / Q000453:epidemiology / Q000235:genetics; D014606:Uveitis, Anterior / Q000453:epidemiology / Q000235:genetics*
D017931:DNA Primers; D015796:HLA-B27 Antigen; D000949:Histocompatibility Antigens Class II; D004247:DNA