Determination of fluoroquinolone residues in animal tissues by liquid chromatography.
A simple liquid chromatographic (LC) method was developed for the determination of fluoroquinolones (ciprofloxacin, difloxacin, enrofloxacin and sarafloxacin) in animal tissues. Isolation of fluoroquinolones from biological matrices was performed with 5% trichloroacetic acid-acetonitrile (7:3) solution. For clean-up, solid-phase extraction with an SDBI (styrene-divinylobenzene) cartridge was used. LC analyses were performed with analytical column (LiChrospher 100 RP-8 5 microns) and mobile phase (0.025 M o-phosphoric acid-acetonitrile 70:30, v/v) in ion-pair mode. The whole procedure was validated in intra- and inter-assay reproducibility and accuracy determination by simultaneously assaying of muscle, liver and kidney samples supplemented with fluoroquinolones at the level of 30 and 60 ng/g, respectively. The statistical evaluation demonstrates high absolute recovery (> 80%) and low coefficient of variation (< 10%) for all analysed samples. The detection limits for fluoroquinolones were 5 ng/g in muscle, liver and kidney samples.
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland.
A
A
Posyniak
J
J
Zmudzki
S
S
Semeniuk
J
J
Niedzielska
R
R
Ellis
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D024841:Fluoroquinolones; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013552:Swine
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D002939:Ciprofloxacin; C047225:difloxacin; C047224:sarafloxacin