Adenovirus-mediated gene expression in vivo is enhanced by the antiapoptotic bcl-2 gene.
An adenovirus vector encoding the human Bcl-2 gene (hBcl-2) was derived. In vivo expression of hBcl-2 in murine livers enhanced and prolonged adenovirus-mediated gene expression. Furthermore, in the hBcl-2-treated group a significant reduction in the apoptosis induced by the adenovirus vector was observed. Thus, the cytoprotection of the vector-infected cells with antiapoptotic genes appears promising for successful in vivo gene therapy.
Bilbao
G
G
Gene Therapy Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Contreras
J L
JL
Zhang
H G
HG
Pike
M J
MJ
Overturf
K
K
Mikheeva
G
G
Krasnykh
V
V
Curiel
D T
DT
D000260:Adenoviruses, Human / Q000502:physiology; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*
D016207:Cytokines; D019253:Proto-Oncogene Proteins c-bcl-2; D001219:Aspartate Aminotransferases