Decreased glomerular proteinase activity in the streptozotocin diabetic rat.
Decreased glomerular proteinase activity may contribute to matrix accumulation in diabetes. Male Sprague-Dawley rats were rendered diabetic by injection of streptozotocin (STZ) 65 mg/kg i.v.; age-matched, sham-injected rats served as controls. Glomeruli from diabetic rats 1 month after STZ injection demonstrated significant decreases in collagenase and cathepsin B activities compared to control glomeruli. Treatment with insulin resulted in a slight (but not significant) increase in collagenase activity and normalized cathepsin B activity. We conclude that decreased glomerular collagenase and cathepsin B activities are present in STZ diabetes. These alterations may contribute to mesangial matrix accumulation.
Department of Medicine, Veterans Affairs Hospital, Hines, Ill., and Loyola University Chicago Stritch School of Medicine, Maywood, Ill., USA.
R H
RH
Song
A K
AK
Singh
D J
DJ
Leehey
D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D007678:Kidney Glomerulus / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D002401:Cathepsin B; D017364:Collagenases