Low responsiveness to immunization with immunoglobulin E/antigen and immunoglobulin G/antigen complexes in H-2Ab mice.
Immunoglobulin (Ig)E and IgG antibodies specific for 2,4, 6-trinitrophenyl (TNP) are able to enhance the carrier-specific antibody response to TNP-conjugated soluble proteins such as bovine serum albumin (BSA). We have recently reported that mice carrying the MHC class II Ab molecule are low responders to immunization with IgE/antigen complexes and now show that H-2Ab mice are also low responders to IgG/antigen complexes. In addition, we found that spleen cells from naive low- and high-responder mice captured IgE/antigen complexes exclusively on B cells, and that the binding was completely inhibited by monoclonal antibodies (MoAbs) against the low-affinity receptor for IgE (FcepsilonRII or CD23). The IgG/antigen complexes were targeted both to B cells and macrophages. The binding of IgG/antigen to B cells primarily seemed to be dependent on the low-affinity receptor for IgG (FcgammaRII or CD32), although some influence of complement receptor 2 (CR2 or CD21) was seen. Capture of IgG/antigen complexes on macrophages was partially blocked by MoAbs against FcgammaRII/III. There was no difference in expression of FcepsilonRII, FcgammaRII/III, CR1, CR2, and CR3 between low- and high-responder strains, thus excluding low levels of these FcRs and CRs as a reason for low responsiveness in H-2Ab mice.
Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden.
S
S
Gustavsson
S
S
Chomez
B
B
Heyman
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000276:immunology*; D002417:Cattle; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology*; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D017463:Receptors, Complement 3b / Q000096:biosynthesis / Q000276:immunology; D017464:Receptors, Complement 3d / Q000096:biosynthesis / Q000276:immunology; D017455:Receptors, IgE / Q000096:biosynthesis / Q000276:immunology; D017452:Receptors, IgG / Q000096:biosynthesis / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D014301:Trinitrobenzenes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006183:H-2 Antigens; C108513:H-2A antigen; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D016177:Macrophage-1 Antigen; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D017455:Receptors, IgE; D017452:Receptors, IgG; D014301:Trinitrobenzenes; C108503:antigen H-2A(k); C038601:trinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens