Rapid determination of aspartate enantiomers in tissue samples by microdialysis coupled on-line with capillary electrophoresis.
Microdialysis was coupled on-line with derivatization by o-phthalaldehyde and beta-mercaptoethanol and optically gated capillary electrophoresis to determine D- and L-aspartate in tissue samples obtained from rats. The microdialysis probe was inserted into a homogenized tissue sample which allowed generation of a continuous sample stream that was filtered and deproteinated. With 7.5 mM beta-cyclodextrin (CD) in the electrophoresis buffer, the enantiomers of interest could be resolved in 3 s with an electric field of 2500 V/cm over a separation length of 15 mm. Values of D- and L-aspartate in different tissues agreed well with those obtained by an HPLC procedure that required protein precipitation, centrifugation, and extraction. The speed and compatibility with automation of the microdialysis/CE method may make it a general approach for a variety of applications involving high-throughput analysis or sensorlike operation.
Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
J E
JE
Thompson
T W
TW
Vickroy
R T
RT
Kennedy
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D008297:Male; D008623:Mercaptoethanol; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism; D009764:o-Phthalaldehyde
D001224:Aspartic Acid; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde