Is another mannose receptor-like lectin present in retinal pigment epithelium?
OBJECTIVE
This study was designed to investigate the presence of the secretory phospholipase A2 receptor in RPE, a protein closely related to the phagocytic mannose receptor.


METHODS
Proteins from cultured rat, pig, and human RPE were separated by SDS-PAGE and immunoblotted with a polyclonal guinea pig sPLA2 receptor antibody. RT-PCR was performed on rat and pig RPE samples using primers designed from published rat pancreatic sPLA2 receptor sequences.


RESULTS
The sPLA2 receptor protein was not detected in rat, pig, or human RPE by immunoblots. Additionally, message for this receptor was not detected in rat or pig RPE.


CONCLUSIONS
With these techniques, these data demonstrate that the sPLA2 receptor is undetectable in the RPE.
Department of Anatomical Sciences & Neurobiology, University of Louisville School of Medicine, KY 40202, USA.
S D
SD
Wilt
B J
BJ
McLaughlin
D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D037102:Lectins; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D010741:Phospholipases A; D054467:Phospholipases A2