Integration of cytogenetic with recombinational and physical maps of mouse chromosome 16.
To link the cytogenetic map for mouse chromosome 16 (Chr 16) to the more detailed recombinational and physical maps, multiple probes were mapped by fluorescence in situ hybridization (FISH). Sixteen large insert clones (YACs, BACs, and PACs) containing markers that have been assigned to the Chr 16 recombinational map were localized to a cytogenetic band or subband by high-resolution FISH. This linkage of the cytogenetic and recombinational maps provides a useful tool for assigning new probe locations and for defining breakpoints in mice with chromosomal rearrangements. A direct application of these probes is demonstrated by identifying mice trisomic for distal Chr 16 using FISH analysis of interphase nuclei.
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
C S
CS
Moore
J S
JS
Lee
B
B
Birren
G
G
Stetten
L L
LL
Baxter
R H
RH
Reeves
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000235:genetics*; D002877:Chromosomes, Human / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008815:Mice, Inbred Strains; D020161:Physical Chromosome Mapping; D011995:Recombination, Genetic
D005819:Genetic Markers