Sequence analysis of the matrix/nucleocapsid gene region of turkey coronavirus.
A reverse transcriptase, polymerase chain reaction (RT-PCR) procedure was used to amplify a segment of the genome of turkey coronavirus (TCV) spanning portions of the matrix and nucleocapsid (MN) protein genes (approximately 1.1 kb). The MN gene region of three epidemiologically distinct TCV strains (Minnesota, NC95, Indiana) was amplified, cloned into pUC19, and sequenced. TCV MN gene sequences were compared with published sequences of other avian and mammalian coronaviruses. A high degree of similarity (>90%) was observed between the nucleotide, matrix protein, and nucleocapsid protein sequences of TCV strains and published sequences of infectious bronchitis virus (IBV). The matrix and nucleocapsid protein sequences of TCV had limited homology (<30%) with MN sequences of mammalian coronaviruses. These results demonstrate a close genetic relationship between the avian coronaviruses, IBV and TCV.
Department of Microbiology, Pathology, and Parasitology, North Carolina State University, Raleigh, NC 27606, USA.
J J
JJ
Breslin
L G
LG
Smith
F J
FJ
Fuller
J S
JS
Guy
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017934:Coronavirus / Q000737:chemistry / Q000235:genetics; D004752:Coronavirus, Turkey / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D001351:Infectious bronchitis virus / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017385:Sequence Homology; D014763:Viral Matrix Proteins / Q000235:genetics*
D012367:RNA, Viral; D014763:Viral Matrix Proteins