Co-expression of 3-ketoacyl-ACP reductase and polyhydroxyalkanoate synthase genes induces PHA production in Escherichia coli HB101 strain.
The Escherichia coli 3-ketoacyl-ACP reductase gene (fabGEc) was cloned using a PCR technique to investigate the metabolic link between fatty acid metabolism and polyhydroxyalkanoate (PHA) production. Three plasmids respectively harboring fabGEc and the poly-3-hydroxyalkanoate synthesis genes phaCAc and phaC1Ps from Aeromonas caviae and Pseudomonas sp. 61-3 respectively were constructed and introduced into E. coli HB101 strain. On a two-stage cultivation using dodecanoate as the sole carbon source, recombinant E. coli HB101 strains harboring fabGEc and phaC genes accumulated PHA copolymers (about 8 wt% of dry cell weight) consisting of several (R)-3-hydroxyalkanoate units of C4, C6, C8, and C10. It has been suggested that overexpression of the fabGEc gene leads to the supply of (R)-3-hydroxyacyl-CoA for PHA synthesis via fatty acid degradation.
Polymer Chemistry Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
K
K
Taguchi
Y
Y
Aoyagi
H
H
Matsusaki
T
T
Fukui
Y
Y
Doi
D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D047908:Intracellular Signaling Peptides and Proteins; D007848:Laurates / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
D001426:Bacterial Proteins; D002352:Carrier Proteins; D005227:Fatty Acids; D047908:Intracellular Signaling Peptides and Proteins; D007848:Laurates; C117249:PhaF protein, Pseudomonas oleovorans; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase