Plant cell growth and differentiation may involve GAP regulation of Rac activity.
Two Rac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus. Two-hybrid screening with dominant-constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho-GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules.
Department of Structural and Molecular Biology, University of Aarhus, Aarhus C, Denmark.
S
S
Borg
L
L
Pødenphant
T J
TJ
Jensen
C
C
Poulsen
D000595:Amino Acid Sequence; D002454:Cell Differentiation; D018076:DNA, Complementary / Q000235:genetics; D004789:Enzyme Activation; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008024:Ligands; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D020744:rac GTP-Binding Proteins; D020703:ras GTPase-Activating Proteins
D018076:DNA, Complementary; D008024:Ligands; D010940:Plant Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins