The influence of individual susceptibility in pyrethroid exposure.
The aim of this study was to find a suitable biomarker for pyrethroid adverse effects. It was shown that there is a correlation between the half-life time (t(1/2)) of pyrethroids in plasma and the clinical findings. We hypothized that this finding indicates an interindividual different amount of total esterase activity or even a polymorphism. By in vitro experiments it was demonstrated that pyrethroids are cleaved by carboxylesterases. After it turned out that carboxylesterase activity in human plasma is too low for detection, a method for specific determination of carboxylesterase activity in human isolated lymphocytes was developed. As a substrate for carboxylesterase activity, cyfluthrin was added to the lymphocyte suspension. As a proof for cyfluthrin degradation by carboxylesterases the produced hydrocyanic acid was determined by GC/MS. First hints for interindividual differences in carboxylesterase activity in lymphocytes were found.
Leng
G
G
Institute of Hygiene, Heinrich-Heine-University Düsseldorf, FRG. leng@uni-duesseldorf.de
Lewalter
J
J
Röhrig
B
B
Idel
H
H
D002265:Carboxylic Ester Hydrolases / Q000097:blood; D006207:Half-Life; D006801:Humans; D007306:Insecticides / Q000009:adverse effects*; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D009570:Nitriles; D016273:Occupational Exposure; D010571:Pest Control; D011722:Pyrethrins / Q000009:adverse effects* / Q000378:metabolism
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D002265:Carboxylic Ester Hydrolases; C052570:cyfluthrin