Immunohistochemical detection of cytokines and cell adhesion molecules in the synovial membrane.
This paper describes the immunohistochemical techniques which can be used to detect cytokines and cell adhesion molecules in synovial membrane tissue, including a list of reagents and possible problems in each technique. It also describes three methods of quantitation of the resultant immunohistochemical detection, including the recent innovation computer-assisted digital video image analysis, and lists the advantages and disadvantages of each quantitation technique. This information will be a useful summary for any scientist interested in applying such techniques to the detection of cytokines and cell adhesion molecules in human tissue sections.
Parker
A
A
Department of Clinical Immunology, Flinders Medical Centre, South Australia.
Smith
M D
MD
D000469:Alkaline Phosphatase / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry; D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism; D019765:Chondroitin ABC Lyase / Q000737:chemistry; D016207:Cytokines / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D013583:Synovial Membrane / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D016707:Tissue Fixation
D000906:Antibodies; D015815:Cell Adhesion Molecules; D016207:Cytokines; D000469:Alkaline Phosphatase; D019765:Chondroitin ABC Lyase