Cell-specific differences in the regulation of IL-6 expression by PMA.
We have studied the regulation of IL-6 expression in human blood monocytes and lymphocytes. LPS and IFN-gamma induced IL-6 gene expression with a similar qualitative profile in both cell types. Treatment of monocytes and lymphocytes with PMA resulted, instead, in different effects: monocytes accumulated IL-6 and its message, while lymphocytes were inhibited either in the absence or the presence of LPS and IFN-gamma. These results suggest that the signal transduction pathways triggered by LPS and IFN-gamma are similar in both cell types, while PMA may activate a tissue-specific pathway which leads to opposite responses.
Costanzo
C
C
Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta' di Medicina e Chirurgia, Universita' di Verona, Strada le Grazie 8, Verona, 37134, Italy.
Piacentini
G
G
Vicentini
L
L
Armenante
F
F
Mazzi
P
P
Savio
C
C
Faggioli
L
L
Boner
A
A
Palmieri
M
M
D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
D017077:Culture Media, Conditioned; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate