Dielectric single particle spectroscopy for measurement of dispersion.
Measuring the frequency-dependent behaviour of single particles or biological cells in inhomogeneous and/or rotating electric fields is a sensitive method for characterising their dielectric properties. This technique is able to detect broad dispersion in the megahertz range of homogeneous artificial Sephadex G15 spheres. Recent progress has opened up the possibility of carrying out dielectric spectroscopy in cell culture media. Dielectrophoretic and electrorotational spectra of different cells in media of varying conductivity can only be explained by the introduction of dispersive cell compartments. The cytoplasm of animal cells typically exhibits a broad dispersion around 15 MHz and there is evidence for membrane dispersion around 50 MHz.
Humboldt-Universität zu Berlin, Institut für Biologie, Lehrstuhl für Membranphysiologie, Germany. ths@rz.hu-berlin.de
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D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D002460:Cell Line; D002468:Cell Physiological Phenomena; D004574:Electromagnetic Fields; D004594:Electrophysiology; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D051379:Mice; D008839:Microelectrodes; D008863:Microspheres; D013057:Spectrum Analysis / Q000379:methods*