A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2.
We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern observed for Wnt-5a at the late gastrula stage. Further we show that gastrula stage Wnt-5a expression is repressed by otx2, something which is reflected by the mutually exclusive expression patterns of these two genes. The isolated promoter sequence contains an OTX- consensus binding site and its' activity in embryos is repressed by ectopically expressed otx2.
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
R
R
Morgan
M H
MH
Hooiveld
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P
In der Reiden
A J
AJ
Durston
D000818:Animals; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins; D006801:Humans; D007763:Lac Operon; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051857:Otx Transcription Factors; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics*; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D014981:Xenopus / Q000196:embryology* / Q000235:genetics*; D029867:Xenopus Proteins
D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; C497659:OTX2 protein, human; D051857:Otx Transcription Factors; C497661:Otx2 protein, Xenopus; D011506:Proteins; D015534:Trans-Activators; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C085096:Wnt5a protein, Xenopus; D029867:Xenopus Proteins