In vivo metabolism of aloemannan.
The metabolism of fluoresceinyl isothiocyanate labeled aloemannan (FITC-AM) was examined by p.o. and i.v. administration in mice at a dose of 120 mg/kg. Analysis of FITC-AM in urine and feces showed that FITC-AM (MW 500 KD) was metabolized into smaller molecules that mainly accumulated in the kidneys. AM was catabolized by the human intestinal microflora to catabolites 1 and 2 with molecular weights of 30 and 10 KD, respectively. Hydrolysis of AM showed hexosamine peaks on HPAE. The findings suggest that the immunomodulation of AM may come from not only neutral polysaccharides but also contaminated hexosamine in AM.
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan. yagi@fupharm.fukuyama-u.ac.jp
A
A
Yagi
J
J
Nakamori
T
T
Yamada
H
H
Iwase
T
T
Tanaka
Y
Y
Kaneo
J
J
Qiu
S
S
Orndorff
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D000504:Aloe; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001711:Biotransformation; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007668:Kidney / Q000378:metabolism; D008351:Mannans / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000494:pharmacology; D051379:Mice; D010946:Plants, Medicinal; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure; D014018:Tissue Distribution; D014466:Ultraviolet Rays
D000276:Adjuvants, Immunologic; D008351:Mannans; C120643:aloemannan; D016650:Fluorescein-5-isothiocyanate