Bioactive surfaces via immobilization of self-assembling polymers onto hydrophobic materials.
Conjugation of proteins to copolymers from poly(acrylic acid) grafted onto PEO-PPO-PEO backbone (Pluronic-PAA) following adsorption of the conjugates onto hydrophobic surfaces is reported. Insulin-Pluronic-PAA conjugates show negligible internalization of insulin into human uterine smooth muscle cells as well as enhancement of mitogenic activity. Glucose-induced release of glycated albumin complexed with a Pluronic-PAA-concanavalin conjugate and adsorbed onto polystyrene nanospheres may provide a model for a glucose-responsive protein delivery system or a heterogeneous diagnostic device.
Bromberg
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Abbott Laboratories, MediSense Products, Swampscott, MA 01907, USA. cpbrolev@aol.com
Salvati
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D000179:Acrylates; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D009130:Muscle, Smooth / Q000166:cytology; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes; D011485:Protein Binding; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
D000179:Acrylates; D007328:Insulin; D011108:Polymers; D011137:Polystyrenes; D020442:Poloxamer