Characterization of monoamine oxidase isoforms in human islets of Langerhans.
In this paper, we describe the characterization of the expression of monoamine oxidase (MAO) in whole pancreas and in isolated islets of Langerhans from human. Classical monamine oxidase activity assays reveal that both isoforms A & B are present in human pancreas. Two complementary approaches indicated that both MAO A and B are expressed in isolated islet: RT-PCR using specific primers revealed amplification products with the expected size for MAO-A and MAO-B: two peptides corresponding to MAO A (approximately 61 kDa) and B (approximately 55 kDa) were detected using a polyclonal anti MAO-A/MAO-B antiserum. Western blotting and subsequent densitometric analysis indicate that whole and endocrine pancreas express the two isoforms with different relative proportions. Islets appear to express almost twice as much MAO protein as whole pancreas, in near equal proportions of the two isoforms, whereas whole pancreas expresses more MAO-A than the B isoform. The expression of MAO A and B in islets could be the first step toward the characterization of the functional properties of these enzymes in the endocrine pancreas.
Pizzinat
N
N
INSERM U388, Pharmacologie Moleculaire et Physiopathologie Renale, Institut Louis Bugnard, Toulouse, France.
Chan
S L
SL
Remaury
A
A
Morgan
N G
NG
Parini
A
A
D015153:Blotting, Western; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000235:genetics / Q000378:metabolism*; D010627:Phenethylamines / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism
D017931:DNA Primers; D007527:Isoenzymes; D010627:Phenethylamines; C029261:phenethylamine; D012701:Serotonin; D004247:DNA; D008995:Monoamine Oxidase