Changes in blood platelets exposed to UV-B radiation.
The effects of UV-B radiation (312 nm) on the pig-blood platelet secretory process (platelet activation) and platelet lipid peroxidation have been studied. The responses of platelets to UV-B radiation are compared with the response of these cells to thrombin, which is a strong platelet agonist. The obtained results show that exposure of blood platelets to UV-B radiation (1.2 mW/cm2, 0.072-8.64 J/cm2) causes dose-dependent platelet lipid peroxidation (measured as thiobarbituric acid reactive substances, TBARS) and release of adenine nucleotides and proteins from irradiated platelets. The dose-dependent release of platelet compounds from irradiated platelet does not correlate with the activity of platelet lactic dehydrogenase (marker of cell lysis) in the extracellular medium. It seems that UV-B radiation can partly activate platelets by stimulating the platelet secretory process and metabolism of arachidonate.
Department of General Biochemistry, University of Lodz, Poland.
K
K
Bednarska
B
B
Wachowicz
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000528:radiation effects*; D015227:Lipid Peroxidation; D015539:Platelet Activation / Q000187:drug effects / Q000528:radiation effects; D013552:Swine; D013858:Thiobarbiturates / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism; D014466:Ultraviolet Rays
D013858:Thiobarbiturates; D013917:Thrombin; C029684:thiobarbituric acid