Expression of aquaporin 1 (AQP1) water channels in human labial salivary glands.
Aquaporin (AQP) water channels are widely expressed in the membranes of fluid-transporting epithelia. Despite the fact that salivary glands are the site of considerable water movement, relatively little is known about the role of aquaporins in human salivary glands. We have examined the expression of AQP1 in human parotid, sublingual and labial salivary glands. Total RNA was extracted from glandular tissue obtained from surgery or biopsy. The presence of AQP1 mRNA was demonstrated in each of the three glands by RT-PCR using primers specifically designed for human AQP1. The PCR product from the labial gland RNA was further amplified with nested primers and the sequence confirmed by automated fluorescent DNA sequencing. The cleaned first PCR product from these glands was then used as a 32P-labelled hybridization probe in a Northern analysis which confirmed the presence of significant amounts of AQP1 transcript in all three glands. AQP1 expression was also demonstrated in cryosections of human labial glands by immunohistochemistry using peroxidase-linked antibodies. Antibody labelling was most prominent in the capillaries but was also evident in the basal regions of the labial gland acini, and may therefore be associated with the serous demilunes which are believed to be a significant site of fluid movement.
Gresz
V
V
Dept. of Oral Biology, semmelweis University of Medicine, Budapest, Hungary.
Burghardt
B
B
Ferguson
C J
CJ
Hurley
P T
PT
Takács
M
M
Nielsen
S
S
Varga
G
G
Zelles
T
T
Case
R M
RM
Steward
M C
MC
D000328:Adult; D051398:Aquaporin 1; D020346:Aquaporins / Q000235:genetics*; D001789:Blood Group Antigens; D015152:Blotting, Northern; D001834:Body Water / Q000378:metabolism; D002196:Capillaries / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008046:Lip / Q000033:anatomy & histology*; D010306:Parotid Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012470:Salivary Glands, Minor / Q000098:blood supply / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012704:Serous Membrane / Q000378:metabolism; D013361:Sublingual Gland / Q000378:metabolism; D014158:Transcription, Genetic
C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D012333:RNA, Messenger; D051398:Aquaporin 1; D012313:RNA