Vaccination with carbohydrate peptide mimotopes promotes anti-tumor responses.
Tumor-associated carbohydrate (TAC) antigens are important targets in cancer vaccine efforts. Carbohydrates are, however, frequently poor immunogens, in that they are T-cell-independent antigens. Molecular mimicry of TAC by peptides is an alternative approach to generating anti-carbohydrate immune responses. Here we demonstrate that peptide mimotopes can elicit antibody responses that cross-react with representative human TAC antigens. Primary immunization with such a multiple antigenic peptide, along with QS-21 as adjuvant, elicits cytotoxic antibodies reactive with naturally occurring forms of TAC expressed on tumor cells, and vaccination of mice with peptide mimotopes reduced tumor growth and prolonged host survival in a murine tumor model.
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA. tom@xray.med.upenn.edu
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Kieber-Emmons
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Luo
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Qiu
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Chang
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Blaszczyk-Thurin
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Steplewski
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy; D014407:Tumor Cells, Cultured; D014611:Vaccination
D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D019496:Cancer Vaccines; D007983:Lewis Blood Group Antigens; D010455:Peptides; C511599:peptide 106; D003165:Complement System Proteins