An Atropa belladonna hyoscyamine 6beta-hydroxylase gene is differentially expressed in the root pericycle and anthers.
The AbH6H gene for hyoscyamine 6beta-hydroxylase (H6H), which converts hyoscyamine to scopolamine, was isolated from Atropa belladonna. This plant also possesses a related sequence, Ab psiH6H, which appears to be a non-functional pseudo-gene. AbH6H RNA was detected in cultured root, native root and anther, but not in stem, leaf, pistil, petal, and sepal tissues. In situ hybridization, immunohistochemistry and promoter::GUS transgene analysis showed that AbH6H is expressed specifically in root pericycle cells, and in tapetum and pollen mother cells. A 671 bp 5'-upstream region from AbH6H was sufficient for pericycle-specific expression in hairy roots of A. belladonna and Hyoscyamus niger, which both produce scopolamine, but cell-specific regulation was severely compromised in tobacco hairy roots, which do not produce scopolamine.
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
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K
Suzuki
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DJ
Yun
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Chen
Y
Y
Yamada
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T
Hashimoto
D000595:Amino Acid Sequence; D001532:Atropa belladonna / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D046508:Culture Techniques; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D015698:Genomic Library; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000033:anatomy & histology / Q000201:enzymology*; D018520:Plant Shoots / Q000033:anatomy & histology / Q000201:enzymology*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011544:Pseudogenes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012601:Scopolamine / Q000096:biosynthesis; D014018:Tissue Distribution; D019076:Transgenes
D012601:Scopolamine; D006899:Mixed Function Oxygenases; C052273:hyoscyamine (6S)-dioxygenase