Differential subcellular immunolocalization of voltage-gated calcium channel alpha1 subunits in the chinchilla cristae ampullaris.
The immunohistochemical localization of alpha1A, alpha1B, alpha1C, alpha1D and alpha1E voltage-gated calcium channel subunits was investigated in the chinchilla cristae ampullaris and Scarpa's ganglia at the light and electron microscopy level with the use of specific antipeptide antibodies directed against these subunits. The stereocilia membrane of type I and type II hair cells was immunoreactive for alpha1B along its entire length. The basolateral membrane of both types of hair cells was alpha1B, alpha1C and alpha1D immunoreactive. Neurons in the Scarpa's ganglia and afferent nerve terminals in the cristae were immunoreactive for alpha1C and alpha1B. No specific immunoreactivity to alpha1A or alpha1E was seen in the sensory epithelia or ganglia. These findings are consistent with the presence of alpha1B (N-type channel), alpha1C and alpha1D (L-type channels) in the vestibular hair cells, and alpha1B (N-type channel) and alpha1C (L-type channel) in primary vestibular neurons.
Department of Surgery (Head and Neck), UCLA School of Medicine, Los Angeles, California, USA.
I
I
López
G
G
Ishiyama
A
A
Ishiyama
J C
JC
Jen
F
F
Liu
R W
RW
Baloh
D000818:Animals; D015220:Calcium Channels / Q000032:analysis*; D002682:Chinchilla; D018069:Hair Cells, Vestibular / Q000737:chemistry* / Q000166:cytology; D007150:Immunohistochemistry; D008297:Male; D014725:Vestibular Nerve / Q000737:chemistry* / Q000166:cytology
D015220:Calcium Channels