Evaluation of Sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures.
We evaluated the Sysmex UF-100 urine flow cytometer (TOA Medical Electronics, Kobe, Japan) with 269 uncentrifuged urine specimens by comparing it with Sternheimer staining and particle counting in 1-microL disposable chambers with both brightfield and phase-contrast microscopy (the reference method). Results of routine test strip analysis, sediment microscopy (182 specimens), and bacterial culture (204 specimens) were also available. Detection of urinary WBCs and RBCs was highly reliable with the UF-100 compared with manual chamber counting (r = .98 and .88, respectively). Identification of bacteria was equal to that with visual microscopy of uncentrifuged specimens; sensitivity was 55%, and specificity 90%, compared with bacterial cultures at a cutoff of > 10(3) colony-forming units per milliliter. Renal damage was difficult to evaluate even with manual methods because of the low counts of renal tubular cells and casts; with standard manual Sternheimer-stained sediment analysis, sensitivity was 65% to 69% and specificity 66% to 91%, compared with the uncentrifuged chamber method at a cutoff of 3 and 10 particles per microliter, respectively. Renal damage was demonstrated with the UF-100 with a sensitivity of 26% to 69% and specificity 92% to 94%, compared with chamber counts. Automated urinalysis with the UF-100 urine flow cytometer offers considerable savings in time and labor. When high sensitivity is needed, visual microscopic review should be performed to detect renal disease.
Department of Clinical Chemistry, Tampere University Hospital, Finland.
T T
TT
Kouri
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U
Kähkönen
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K
Malminiemi
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R
Vuento
R M
RM
Rowan
D001322:Autoanalysis / Q000191:economics / Q000295:instrumentation / Q000379:methods; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001437:Bacteriuria / Q000175:diagnosis / Q000382:microbiology*; D002452:Cell Count; D017046:Cost Savings; D004847:Epithelial Cells / Q000166:cytology; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000191:economics / Q000295:instrumentation*; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis / Q000382:microbiology*; D008858:Microscopy, Phase-Contrast; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D014556:Urine / Q000166:cytology / Q000382:microbiology*