Charge derivatization of peptides to simplify their sequencing with an ion trap mass spectrometer.
The low energy collision-induced dissociation of fixed-charge derivatives [tris(2,4,6-trimethoxyphenyl)phosphonium] of peptides was investigated using an electrospray ion trap mass spectrometer. The fixed charge directed the fragmentation pattern and generated solely N-terminal fragments with minimal internal rearrangement, regardless of the presence and position of basic amino acids in the peptide chain. Generally only b-type ions, accompanied by less intense a-type ions, were observed, depending on the collision energy. It was observed that the fixed charge controlled the fragmentation beyond typical MS/MS, and thus the capacity of the ion trap to perform multiple stage fragmentation (MS(n)) was found particularly useful for obtaining the complete sequence information of the peptides.
Adamczyk
M
M
Department of Chemistry (9NM), Abbott Diagnostic Division, Abbott Laboratories, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Gebler
J C
JC
Wu
J
J
D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D017421:Sequence Analysis / Q000379:methods*; D055672:Static Electricity
D009943:Organophosphorus Compounds; D010455:Peptides