Mother-to-infant transmission of hepatitis C virus: molecular evidence of superinfection by homologous virus in children.
OBJECTIVE
Vertical transmission of hepatitis C virus (HCV) is well established but its incidence is low. To assess the molecular evidence of mother-to-infant transmission or intrafamilial transmission of HCV, the NS5 B region and the hypervariable region 1 (HVR1) of the E2/NS1 region of the HCV genome from each member of a family were investigated.


METHODS
A 35-year-old mother with chronic hepatitis C virus infection and her four infected boys were studied. The same HCV 1a genotype was found in all five. Phylogenetic analysis was done by the neighbor-joining, the maximum likelihood, and the maximum parsimony methods.


RESULTS
Comparison of the phylogenetic trees in the NS5B and HVR1 regions showed that the sequences in the children were more closely related to the population of variants of their own mother than to any genotype la sequence available in the databases. However, four HVR1 clones from two brothers (E2 and E3) had a strong homology, but were significantly divergent from the variants of the mother.


CONCLUSIONS
These results suggest that a cluster of HCV strains exists in the family and that E3 could have been superinfected by E2 HCV strains and reciprocally. In conclusion, phylogenetic analysis through variable regions of the genome suggests that at least two modes of transmission are involved in this family: perinatal and horizontal.
Halfon
P
P
Laboratoire de Biochimie, Hôpital de la Conception, CNRS, Marseilles, France. alphabio@wanadoo.fr
Quentin
Y
Y
Roquelaure
B
B
Sarles
J
J
Halimi
G
G
Gerolami
V
V
Khiri
H
H
Bourlière
M
M
Cartouzou
G
G
D000328:Adult; D002648:Child; D002675:Child, Preschool; D018562:Disease Transmission, Infectious; D005260:Female; D006515:Hepatitis B virus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000209:etiology* / Q000821:virology; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D012367:RNA, Viral / Q000032:analysis; D017385:Sequence Homology; D015163:Superinfection
D012367:RNA, Viral