Endothelial activation and response in patients with hand arm vibration syndrome.
BACKGROUND
Hand-arm vibration syndrome (HAVS) is a form of secondary Raynaud's phenomenon (RP) of occupational origin. In other forms of RP, blood and blood vessel wall interaction is one factor in the pathophysiology. Cytokines and cell adhesion molecules both play an important role in this interaction, and basal vascular tone and vasodilatation are regulated by nitric oxide.


METHODS
Blood flow responses to acetylcholine (ACh) and sodium nitroprusside (SNP) and levels of soluble intercellular adhesion molecule-1 (sICAM-1) and the inflammatory cytokine interleukin 8 (IL-8) were measured in eight male patients with vibration white finger disease, which is part of HAVS, and in eight healthy matched male control subjects.


RESULTS
sICAM-1 levels were statistically higher (P = 0.02, Mann-Whitney U-test) and IL-8 levels (P < 0.01, Mann-Whitney) were significantly lower in the patient group. The patients with HAVS had significantly reduced vascular responses to SNP (P < 0.05, ANOVA).


CONCLUSIONS
In this study, we reveal differences in vascular responses to SNP that suggest there may be an impairment of the smooth muscle response to nitric oxide in patients with HAVS. The increase in sICAM-1 that occurs in patients with HAVS suggests that leucocyte adhesion is increased and that adherent neutrophils may contribute to the microvascular damage seen in this disease. The impeded flow of blood cells through the microcirculation may result in the low levels of circulating IL-8 due to the cytokine binding to erythrocytes. The possible role of NO activity in HAVS warrants further investigation.
Section of Vascular Medicine & Biology, University Department of Medicine, Ninewells Hospital & Medical School, Dundee, UK. G.KIRK@dundee.ac.uk
G
G
Kennedy
F
F
Khan
M
M
McLaren
J J
JJ
Belch
D000109:Acetylcholine / Q000494:pharmacology; D000328:Adult; D000368:Aged; D001132:Arm; D001783:Blood Flow Velocity / Q000187:drug effects; D004730:Endothelium, Vascular / Q000502:physiology / Q000503:physiopathology*; D005385:Fingers; D006225:Hand; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009599:Nitroprusside / Q000494:pharmacology; D011928:Raynaud Disease / Q000503:physiopathology*; D012016:Reference Values; D014732:Vibration
D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1; D009599:Nitroprusside; D000109:Acetylcholine