The C. elegans gene lin-36 acts cell autonomously in the lin-35 Rb pathway.
The Caenorhabditis elegans gene lin-36 acts to antagonize Ras-mediated vulval induction in a pathway that includes genes with products similar to the mammalian retinoblastoma (Rb) protein and the Rb-binding protein p48. We report that lin-36 encodes a novel protein of 962 amino acids. We demonstrate that lin-36 functions in and is expressed in the vulval precursor cells, establishing that the lin-36 pathway is involved in intercellular signaling. We also report that the lin-36 pathway and/or another pathway that is functionally redundant with the lin-36 pathway antagonize a ligand-independent activity of the receptor tyrosine kinase/Ras vulval induction pathway.
Howard Hughes Medical Institute, Department of Biology, Room 68-425, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
J H
JH
Thomas
H R
HR
Horvitz
D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development*; D029742:Caenorhabditis elegans Proteins; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D016161:Genes, Retinoblastoma; D015801:Helminth Proteins / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D012097:Repressor Proteins / Q000235:genetics; D016160:Retinoblastoma Protein / Q000235:genetics*; D015398:Signal Transduction; D014844:Vulva / Q000254:growth & development
D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; C116485:lin-35 protein, C elegans; C121170:lin-36 protein, C elegans; D066246:ErbB Receptors; C085873:let-23 protein, C elegans