Bone modeling and cell-material interface responses induced by nickel-titanium shape memory alloy after periosteal implantation.
The purpose of this study was to evaluate the new bone formation, modeling and cell-material interface responses induced by nickel-titanium shape memory alloy after periosteal implantation. We used a regional acceleratory phenomenon (RAP) model, in which a periosteal contact stimulus provokes an adaptive modelling response. NiTi has thermal shape memory and superelasticity properties uncommon in other implant alloys. So far, there are insufficient data concerning the biocompatibility of NiTi as a bone implant. NiTi was compared to stainless steel (stst) and Ti-6Al-4V. The test implant was placed in contact with the intact femur periosteum, but it was not fixed inside the bone. Histomorphometry with digital image analysis was used to determine the bone formation and resorption parameters. The ultrastructural features of cell-material adhesion were analysed with scanning electron microscopy (FESEM). A typical peri-implant bone wall modelation was seen due to the normal RAP. The maximum new woven bone formation started earlier (2 weeks) in the Ti-6Al-4V group than in the NiTi (P < 0.01) group, but also decreased earlier, and at 8 weeks the NiTi (P < 0.05) and stst (P < 0.005) groups had greater cortical bone width. At 12 and 26 weeks no statistical differences were seen in the histomorphometric values. The histological response of the soft tissues around the NiTi implant was also clearly non-toxic and non-irritating. Cell adhesion and focal contacts were similar between the materials studied by FESEM. We conclude that NiTi had no negative effect on total new bone formation or normal RAP after periosteal implantation during a 26-week follow-up.
Department of Surgery, Oulu University Central Hospital, University of Oulu, Finland. jorma.ryhanen@oulu.fi
J
J
Ryhänen
M
M
Kallioinen
J
J
Tuukkanen
P
P
Lehenkari
J
J
Junila
E
E
Niemelä
P
P
Sandvik
W
W
Serlo
D000497:Alloys; D000818:Animals; D001861:Bone Regeneration; D016723:Bone Remodeling; D018786:Bone Substitutes; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion; D002462:Cell Membrane / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D009532:Nickel; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D014025:Titanium
D000497:Alloys; D018786:Bone Substitutes; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium