The Feulgen reaction 75 years on.
The Feulgen reaction proposed by Feulgen and Rossenbeck 75 years ago is one of the cytohistochemical reactions most widely used in biology and medicine. It allows DNA in situ to be specifically stained based on the reaction of Schiff or Schiff-like reagents with aldehyde groups engendered in the deoxyribose molecules by HCl hydrolysis. The staining intensity is proportional to the DNA concentration. Current applications of the Feulgen reaction are mainly concerned with DNA quantification in cell nuclei by image cytometry for ploidy evaluation in tumor pathology. From the morphological point of view, specific demonstration of DNA in cell structures at the light microscopic level is very little used nowadays. On the other hand, application of the Feulgen principles to electron microscopy have recently allowed specific DNA-staining procedures to be developed for the study of the structural organization of DNA in situ.
Chieco
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Istituto di Oncologia F. Addarii, Policlinico S. Orsola, Bologna, Italy.
Derenzini
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D000818:Animals; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D006801:Humans; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA