New substrates of DNA polymerases.
Bis-(2'-deoxynucleoside) 5',5'-tetraphosphates and bis-(2'-deoxynucleoside) 5',5'-triphosphates were shown to be a new type of substrate for several DNA polymerases of human, bacterial and viral origin. Their substrate properties depend both on their structure and on the nature of the enzyme. They are incorporated by both termini in correspondence with the template nucleotide program in the active center. The results obtained support the mechanism of their direct incorporation rather than prior hydrolysis to dNTP. The highest activity of these compounds was observed for HIV reverse transcriptase. The probable biological significance of the reaction is discussed.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
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L
Victorova
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Sosunov
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Skoblov
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Shipytsin
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Krayevsky
D001419:Bacteria / Q000201:enzymology; D004256:DNA Polymerase I / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013379:Substrate Specificity; D019914:Taq Polymerase / Q000378:metabolism; D014780:Viruses / Q000201:enzymology
D015226:Dinucleoside Phosphates; D019914:Taq Polymerase; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase