Rapid high-performance liquid chromatographic determination of cefepime in human plasma.
A simple, rapid and reproducible high-performance liquid chromatographic method for the quantitative determination of cefepime in human plasma was developed. Ceftazidime was used as internal standard. Chromatography was performed on a reversed-phase encapped column (Hypersil BDS C18). The samples, after protein precipitation, were eluted with a mobile phase of acetonitrile-acetate buffer, pH 4 (2.8:97.2, v/v). The detection wavelength was 254 nm. The limit of quantitation of cefepime was 0.5 microgram/mL and only 0.5 mL of plasma sample was required for the determination. The average cefepime recoveries over a concentration range of 0.5-500 micrograms/mL ranged from 98 to 104%. Precision and accuracy did not exceed 5%.
Department of Clinical Pharmacology, Clinica Universitaria de Navarra, Pamplona, Spain.
B
B
Calahorra
M A
MA
Campanero
B
B
Sádaba
J R
JR
Azanza
D000077723:Cefepime; D002511:Cephalosporins / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002511:Cephalosporins; D000077723:Cefepime