Weak effect of neuromodulators on climbing fiber-activated [Ca(2+)](i) increases in rat cerebellar Purkinje neurons.
The effect of several neuromodulators (carbachol (CCh), serotonin (5-HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)-induced [Ca(2+)](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca(2+) indicator bis-fura-2 with patch electrodes on the soma. [Ca(2+)](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF-induced [Ca(2+)](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.
Pisani
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Clinica Neurologica, Università di Roma Tor Vergata, 8-00173, Rome, Italy.
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D000818:Animals; D002118:Calcium / Q000378:metabolism*; D009412:Nerve Fibers / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D018377:Neurotransmitter Agents; D002118:Calcium