Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect cell aggregates.
The Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell line has received considerable attention as a host for the baculovirus expression vector system. In the present study, suspension cultures were used to compare Tn-5B1-4 cell aggregates and cells selected to grow predominantly as individual cells. No significant difference was found between cell aggregates and cells growing predominantly individually in regard to cell growth rate, glucose consumption and lactate accumulation, and specific recombinant protein synthesis levels. In addition, the levels of recombinant protein synthesis were considerably higher than those produced by the commonly used Spodoptera frugiperda Sf-9 insect cell line.
Department of Chemical and Biochemical Engineering, University of Iowa, 125B Chemistry Building, Iowa City, Iowa 52242-1219, USA.
M A
MA
Saarinen
K A
KA
Troutner
S G
SG
Gladden
C M
CM
Mitchell-Logean
D W
DW
Murhammer
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009036:Moths / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D019344:Lactic Acid; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase; D005947:Glucose