[Assay and detection methods for urokinase-type plasminogen activator and its related-factors].
The urokinase-type plasminogen activator (u-PA) has been implicated in invasion and metastasis of various cancers including lung, breast, gastric and colon cancers. The u-PA system consists of plasmin, u-PA, specific u-PA receptor and the serpins, PAI-1 and PAI-2. It has been demonstrated that the reciprocal actions among the components of the u-PA system are much important for regulating u-PA activity. Recent studies are focusing on the interaction between the u-PA system and cell adhesion molecules such as integrins, and the physiological significance in vivo of u-PA system-related proteins. We introduce here the useful methods to examine the u-PA enzyme activity and the expression of proteins and genes related with the u-PA system for clinical cancer specimens.
Department of Experimental Therapeutics, Kanazawa University, Japan.
K
K
Kimura
Y
Y
Endo
T
T
Sasaki
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016023:Integrins / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000032:analysis* / Q000235:genetics; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D016023:Integrins; D012333:RNA, Messenger; D015843:Serpins; D014568:Urokinase-Type Plasminogen Activator