Redesignation of a purported P1.15 subtype-specific meningococcal monoclonal antibody as a P1.19-specific reagent.
Two reference monoclonal antibodies against the meningococcal P1.15 subtype PorA, MN3C5C and 2-1-P1.15, showed only partial concordant recognition of meningococcal isolates. Cyanogen bromide cleavage of P1.19,15 PorA, peptide mapping, and sequencing of porA regions demonstrated that 2-1-P1.15 was specific for subtype P1.19, and henceforth it is to be redesignated as 2-1-P1.19.
Wedege
E
E
Department of Vaccinology, Department of Bacteriology, National Institute of Public Health, N-0403 Oslo, Norway. elisabeth.wedege@folkehelsa.no
Caugant
D A
DA
Musacchio
A
A
Saunders
N B
NB
Zollinger
W D
WD
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000276:immunology*; D018272:Porins / Q000737:chemistry* / Q000235:genetics
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D018272:Porins; C057699:porin protein, Neisseria