p130cas is a cellular target protein for tyrosine nitration induced by peroxynitrite.
We found that the exposure of human neuroblastoma SH-SY5Y cells to the peroxynitrite donor 3-morpholinosydnonimine (SIN-1) induced tyrosine nitration of a 130-kDa protein, and prevented tyrosine phosphorylation of the 130-kDa protein. The focal adhesion protein p130cas was identified as a component of the 130-kDa protein using specific antibody. These results suggest that p130cas is a new target protein for nitration induced by SIN-1.
Department of Pharmacology, Faculty of Dentistry, Osaka University, Suita, Japan.
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Saeki
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Maeda
D050719:Crk-Associated Substrate Protein; D006801:Humans; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009447:Neuroblastoma; D009566:Nitrates / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010750:Phosphoproteins / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
C493841:BCAR1 protein, human; D050719:Crk-Associated Substrate Protein; D009566:Nitrates; D020030:Nitric Oxide Donors; D016877:Oxidants; D010750:Phosphoproteins; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; C039642:peroxynitric acid; C002744:3-nitrotyrosine; D014443:Tyrosine; C002385:linsidomine; D008981:Molsidomine