[Effect of arsenite on the physiological and cytological properties of Pseudomonas putida strains capable of degrading polycyclic aromatic hydrocarbons].
Some physiological and cytological properties of Pseudomonas putida strains resistant to arsenite and capable of degrading polycyclic aromatic hydrocarbons were studied. The resistance of P. putida BS202 (NPL-1) to arsenite proved to be determined by chromosomal genes, while the arsenite resistance of P. putida BS238 (pBS2; pBS3031) was by plasmid-borne genes. Arsenite affected the pattern and rate of growth of strain BS202 (NPL-1) in media with naphthalene or salicylate as carbon sources; particularly, it lengthened the lag phase. Electron-microscope analysis of the strains studied did not reveal any arsenite-induced destructive changes in the cell envelope. At the same time, arsenite in the growth medium induced some alterations in the structure of the outer membrane of strain BS202 (NPL-1) and the cytoplasmic membrane of strain BS238 (pBS2; pBS3031) and, in both strains, led to an increase in the density of intramembrane particles on the EF face of the freeze-fractured cytoplasmic membrane. Arsenite resistance probably evidently protects cells of both strains from greater damage. Physiological and cytological data suggest that the mechanisms of arsenite resistance in the strains studied are different.
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow oblast, Russia.
E V
EV
Kozlova
N E
NE
Suzina
A V
AV
Karpov
V I
VI
Duda
A M
AM
Boronin
D018053:Arsenites / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D005614:Freeze Fracturing; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D016958:Pseudomonas putida / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013379:Substrate Specificity
D018053:Arsenites; D011084:Polycyclic Aromatic Hydrocarbons; C015001:arsenite
Vliianie arsenita na fiziologicheskie i tsitologicheskie svoĭstva shtammov Pseudomonas putida, destruktorov politsiklicheskikh aromaticheskikh uglevodorodov.