[Effect of conditions od Trichosporon pullulans culturing on the synthesis of immunomodulating glycoprotein].
An extracellular glycoprotein (GP) exhibiting immunomodulating activity produced by the yeast Trichosporon pullulans grown in a defined ethanol-containing medium differed substantially in its composition from that of the yeast cell walls: therefore, it cannot be considered a structural component of the cell walls. In batch culture, the greatest GP production (40 mg/l) occurred in the exponential phase of the yeast growth. Under continuous cultivation, in both chemostat and pH-auxostat regimes, the specific rate of GP synthesis (qGP) increased with the increasing specific growth rate (mu) and reached 1.55 mg/(g h) at mumax. Under limitation of the yeast growth by zinc qGP was three times lower than under nitrogen or iron limitation. The rate of GP production depended inversely on the oxygen concentration.
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow oblast, Russia.
V K
VK
Eroshin
E G
EG
Dediukhina
V M
VM
Vagabov
I S
IS
Kulaev
D000276:Adjuvants, Immunologic / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology; D014250:Trichosporon / Q000254:growth & development* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D000596:Amino Acids; D006023:Glycoproteins
Vliianie usloviŭ kul'tivirovaniia Trichosporon pullulans na sintez glikoproteina-immunomoduliatora.