A novel A-kinase anchoring protein in the heart interacts with G alpha 13.
A cDNA of a tentative A-kinase anchoring protein, presumably coupled with heterotrimeric GTP binding protein alpha 13 subunit (G alpha 13), was cloned from a human heart cDNA library. It was approximately 650 bases and its mRNA was expressed in the heart. Homology search of DNA sequences revealed that it was a novel cDNA with 84% homology with the partial sequence of rabbit cDNA of AKAP 120 without a stop codon. 3'-Rapid Amplification of cDNA Ends (3'-RACE) and yeast functional assay were performed to determine the 3'-end of the cDNA and ribosomal frameshifting was suggested as a translational mechanism. Here we report that a protein encoded by the cDNA may be involved in intracellular signal transduction via the G alpha 13 and PKA in hearts.
Department of Cardiovascular Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
M
M
Suzuki
K
K
Furuuchi
H
H
Tonoki
T
T
Ozaki
K
K
Iizuka
T
T
Murakami
A
A
Kitabatake
H
H
Kawaguchi
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics*; D017421:Sequence Analysis; D015398:Signal Transduction / Q000235:genetics*; D015003:Yeasts / Q000235:genetics*
D054758:A Kinase Anchor Proteins; C119719:AKAP9 protein, human; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D011506:Proteins