Elevated plasma levels of F2 alpha isoprostane in cystic fibrosis.
Cystic fibrosis (CF) is associated with chronic lung infection, inflammation, and elevated indices of oxidative stress. Recently, isoprostanes were shown to be a reliable in vivo marker of oxidant injury with 8-iso-PGF2 alpha, shown to cause airflow obstruction and plasma exudation in guinea pig lung. The present study was designed to examine the relationship between 8-iso-PGF2 alpha levels, plasma antioxidants, and clinical status in CF. We hypothesized that plasma 8-iso-PGF2 alpha levels would be higher in subjects with CF compared to healthy controls. Plasma 8-iso-PGF2 alpha levels were prospectively measured in 22 subjects with CF and nine healthy controls using an 8-isoprostane enzyme immunoassay kit along with plasma vitamins A, E, and beta-carotene. Plasma 8-iso-PGF2 alpha levels were shown to be significantly elevated in the CF subjects compared to controls (319.6 +/- 52.6 vs. 145.0 +/- 21.0 pg/mL, P = 0.005). Plasma levels of antioxidants were significantly lower for the CF subjects compared to the controls (vitamin A, P < 0.003; vitamin E, P < 0.001; and beta-carotene, P < 0.01). This study confirms significantly elevated lipid peroxidation in CF using 8-iso-PGF2 alpha levels.
Collins
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Department of Dietetics, John Hunter Children's Hospital, Rankin Park, New South Wales, Australia. ccollins@mail.newcastle.edu.au
Quaggiotto
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Wood
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O'Loughlin
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Henry
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Garg
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D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D003550:Cystic Fibrosis / Q000097:blood*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism; D028441:F2-Isoprostanes; D005227:Fatty Acids / Q000097:blood; D006801:Humans; D008875:Middle Aged; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene / Q000097:blood
D028441:F2-Isoprostanes; D005227:Fatty Acids; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost