Roles of vasopressin and hypertonicity in basolateral Na/K/2Cl cotransporter expression in rat kidney inner medullary collecting duct cells.
The basolateral Na/K/2Cl cotransporter mRNA (rNKCC1) increased when the cultured kidney inner medullary collecting duct (IMCD) cells of rats were exposed to vasopressin (10(-8) M) and/or hypertonicity (500 mOsm/kgH2O). However, only hypertonicity was effective in increasing the expression of rNKCC1 protein.
Department of Physiology and *Department of Internal Medicine, Kitasato University School of Medicine, Sagamihara, 228-8555, Japan.
N
N
Anzai
I
I
Izumida
Y
Y
Kobayashi
K
K
Kawahara
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D006982:Hypertonic Solutions / Q000494:pharmacology*; D007679:Kidney Medulla / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012076:Renal Agents / Q000494:pharmacology*; D028021:Sodium-Potassium-Chloride Symporters; D014667:Vasopressins / Q000494:pharmacology*
D002352:Carrier Proteins; D006982:Hypertonic Solutions; D012333:RNA, Messenger; D012076:Renal Agents; D028021:Sodium-Potassium-Chloride Symporters; D014667:Vasopressins