In vivo characterization of keratinocyte growth factor-2 as a potential wound healing agent.
Human keratinocyte growth factor-2 exerts a proliferative effect on epithelial cells and mediates keratinocyte migration. It has also been shown to increase both deposition of granulation tissue and collagen and maturation of collagen. Because these properties should affect the healing trajectory of wounds, this study set out to investigate the effects of keratinocyte growth factor-2 on the healing of three different types of wounds. Human meshed skin grafts explanted to athymic "nude" rats, surgical incisions in Sprague-Dawley rats, and acute excisional rat wounds inoculated with Escherichia coli were used. Two concentrations of recombinant human keratinocyte growth factor-2 were compared to a vehicle control and keratinocyte growth factor-1. Keratinocyte growth factor-2 significantly accelerated the rate of epithelialization in the meshed skin graft model and effected a modestly more rapid gain in breaking strength of surgical incisions than keratinocyte growth factor-1 or the vehicle control treatment. Neither keratinocyte growth factors accelerated wound closure by contraction of the excisional wounds. Based on these data, keratinocyte growth factor-2 may be useful in accelerating healing in wounds healing mainly by the process of epithelialization such as venous stasis ulcers, partial thickness burn wounds, and skin graft donor sites. It might also accelerate the gain in incisional wound strength in acute surgical or traumatic wounds.
Institute of Tissue Regeneration, Repair, and Rehabilitation, Bay Pines, Florida 33744, USA.
P M
PM
Soler
T E
TE
Wright
P D
PD
Smith
S P
SP
Maggi
D P
DP
Hill
F
F
Ko
P A
PA
Jimenez
M C
MC
Robson
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000187:drug effects; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects; D004927:Escherichia coli Infections / Q000473:pathology / Q000503:physiopathology; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006097:Granulation Tissue / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D014677:Pharmaceutical Vehicles; D051381:Rats; D011923:Rats, Nude; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000503:physiopathology; D016038:Skin Transplantation; D013314:Stress, Mechanical; D013530:Surgical Wound Infection / Q000473:pathology / Q000503:physiopathology; D014183:Transplantation, Heterologous; D014945:Wound Healing / Q000187:drug effects
C496463:FGF10 protein, human; C496453:FGF7 protein, human; C496465:Fgf10 protein, rat; C496455:Fgf7 protein, rat; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D014677:Pharmaceutical Vehicles; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D003094:Collagen