Methanobacterium thermoautotrophicum RNA polymerase and transcription in vitro.
RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum DeltaH has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor TFB. Efforts to obtain transcription initiation from hydrogen-regulated methane gene promoters were, however, unsuccessful. Two previously unrecognized archaeal RNAP subunits have been identified, and complex formation by the M. thermoautotrophicum RNAP and TFB has been demonstrated.
Darcy
T J
TJ
Department of Microbiology, The Ohio State University, Columbus, Ohio 43210, USA.
Hausner
W
W
Awery
D E
DE
Edwards
A M
AM
Thomm
M
M
Reeve
J N
JN
D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D002499:Centrifugation, Density Gradient; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D019848:Gene Expression Regulation, Archaeal; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D017014:Methanobacterium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011233:Precipitin Tests; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; C106466:transcription factor B, Archaea; D006859:Hydrogen; D012321:DNA-Directed RNA Polymerases; D008697:Methane