Preferential uptake and accumulation of oxidized vitamin C by THP-1 monocytic cells.
THP-1 cells preferentially accumulate vitamin C in its oxidized form. The uptake displays first-order kinetics and leads to a build-up of an outward concentration gradient which is stable in the absence of extracellular vitamin. The transport is faster than reduction by extracellular glutathione or by added cytosolic extract, and glutathione-depleted cells show the same uptake rates as control cells. In addition, energy depletion or oxidation of intracellular sulfhydryls does not inhibit accumulation of ascorbate. The accumulation, however, always occurs in the reduced form. The affinity for dehydroascorbate is lower (Km 450 microM vs 60 microM) than for reduced ascorbate, but the maximal rate is more than 30 times higher (581 compared to 19 pmol.min-1 per 106 cells), and it is independent of sodium, whereas the uptake of ascorbate is not. The sodium gradient also allows accumulation of reduced ascorbate. Inhibitors of glucose transport by the GLUT-1 transporter also inhibit uptake of dehydroascorbate (DHA), but there are some inconsistencies, because the Ki-values are higher than reported for the isolated transporter and one inhibitor (deoxyglucose) is noncompetitive. The preferential uptake of the dehydro-form of the vitamin may be useful for situations where this short-lived metabolite is formed by oxidation in the environment.
Laggner
H
H
Institut für Medizinische Chemie, University of Vienna, Austria.
Besau
V
V
Goldenberg
H
H
D001205:Ascorbic Acid / Q000032:analysis / Q000378:metabolism*; D003683:Dehydroascorbic Acid / Q000032:analysis / Q000378:metabolism*; D003847:Deoxyglucose / Q000494:pharmacology; D004734:Energy Metabolism; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D005292:Ferricyanides / Q000378:metabolism / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007951:Leukemia, Myeloid; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors; D010084:Oxidation-Reduction; D019163:Reducing Agents / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014407:Tumor Cells, Cultured
D005292:Ferricyanides; D009004:Monosaccharide Transport Proteins; D019163:Reducing Agents; D013438:Sulfhydryl Compounds; C007931:hexacyanoferrate III; D003847:Deoxyglucose; D005978:Glutathione; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid