Apoptosis following spinal cord injury in rats and preventative effect of N-methyl-D-aspartate receptor antagonist.
OBJECTIVE
The aims of this study were to clarify the histological and histochemical changes associated with cell death in the spinal cord after acute traumatic injury and to examine the role of excitatory amino acid release mediated by N-methyl-D-aspartate (NMDA) receptors.


METHODS
Following laminectomy, the spinal cord in 70 rats was injured at the T-9 level by applying extradural static weight-compression, in which a cylindrical compressor was used to induce complete and irreversible transverse spinal cord injury (SCI) with paralysis of the lower extremities. The injured rats were killed between 30 minutes and 14 days after injury, and the injured cord was removed en bloc. Rats that received NMDA receptor antagonist (MK-801) were killed at the same time points as those that received saline. The specimens were stained with hematoxylin and eosin, Nissl, and Klüver-Barrera Luxol fast blue and subjected to in situ nick-end labeling, a specific in situ method used to allow visualization of apoptosis. Thirty minutes post-SCI, a large hematoma was observed at the compressed segment. Six hours after injury, large numbers of dead cells that were not stained by in situ nick-end labeling were observed. Between 12 hours and 14 days postinjury, nuclei stained by using the in situ nick-end labeling technique were observed not only at the injury site but also in adjoining segments that had not undergone mechanical compression, suggesting that the delayed cell death was due to apoptosis. The number of cells stained by in situ nick-end labeling was maximum at 3 days postinjury. The results of electron microscopic examination were also consistent with apoptosis. In the MK-801-treated rats, the number of cells stained by in situ nick-end labeling was smaller than in nontreated rats at both 24 hours and 7 days after injury.


CONCLUSIONS
These findings suggest that NMDA-receptor activation promotes delayed neuronal and glial cell death due to apoptosis.
Wada
S
S
Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Sakuragaoka, Japan.
Yone
K
K
Ishidou
Y
Y
Nagamine
T
T
Nakahara
S
S
Niiyama
T
T
Sakou
T
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002452:Cell Count; D016923:Cell Death; D002467:Cell Nucleus / Q000648:ultrastructure; D004396:Coloring Agents; D003711:Demyelinating Diseases / Q000188:drug therapy / Q000473:pathology; D016291:Dizocilpine Maleate / Q000008:administration & dosage / Q000627:therapeutic use*; D018691:Excitatory Amino Acid Antagonists / Q000008:administration & dosage / Q000627:therapeutic use*; D005456:Fluorescent Dyes; D006406:Hematoma / Q000473:pathology; D020287:In Situ Nick-End Labeling; D007796:Laminectomy; D008297:Male; D008854:Microscopy, Electron; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors; D009413:Nerve Fibers, Myelinated / Q000187:drug effects / Q000473:pathology; D009474:Neurons / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D010243:Paralysis / Q000188:drug therapy / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013117:Spinal Cord Compression / Q000473:pathology; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000473:pathology*
D004396:Coloring Agents; D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D018696:Neuroprotective Agents; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate