A novel cleavage protocol for use with Rf-encoded split pool synthesis technology: product cleavage and collection in standard 96 well format.
A novel protocol which employs commercially available, standard tools and hardware has been developed. This protocol allows for cleavage and collection of IRORI Microkan products in 96 well plate format. Typically, 640 compounds can be cleaved in a 4 hour time period using approximately 3 square feet of space. This protocol has been used successfully for the liberation of thousands of individual compounds, in single compound per well format from the solid phase. Additionally, this protocol is the first example of making IRORI Microkan technology directly compatible with standard 96 position deep well blocks.
Andres
C J
CJ
Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, CT 06492, USA.
Swann
R T
RT
Grant-Young
K
K
D'Andrea
S V
SV
Deshpande
M S
MS
D004864:Equipment and Supplies; D006868:Hydrolysis; D019151:Peptide Library
D019151:Peptide Library