Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents.
The recombinant Escherichia coli strain DPD2794 containing a recA::luxCDABE fusion is used to detect genotoxicity of various chemicals. Genotoxic agents were previously categorized into two groups, Direct DNA Damaging (DDD) agents and Indirect DNA Damaging (IDD) agents; these two groups have been distinguished with this strain. Minimum detectable concentrations of the DDD agents were about one to five orders of magnitude lower than those of the IDD agents. The response patterns of this strain to DDD agents differed from those to IDD agents in terms of kinetics and the forms of the dose-dependent response.
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology (K-JIST), 1 Oryong-dong, Puk-gu, Kwangju 500-712, South Korea.
J
J
Min
E J
EJ
Kim
R A
RA
LaRossa
M B
MB
Gu
D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D008163:Luminescent Measurements; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011938:Rec A Recombinases / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D013014:SOS Response, Genetics / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014733:Vibrio / Q000235:genetics
D004269:DNA, Bacterial; D009153:Mutagens; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D011938:Rec A Recombinases