[Effect of oxidized low density lipoproteins on the structure of platelet membrane. Use of electron paramagnetic resonance].
The effect of low-density lipoproteins on the structure of platelet plasma membrane was studied by electron paramagnetic resonance spectroscopy. Low-density lipoproteins were incubated with platelet rich plasma at a volume ratio 1:1. Plasma incubated with buffer served as a control. After incubation, the fluidity of platelet plasma membrane was determined by electron spin resonance probes 5-doxylstearate and 16-doxylstearate, which were immobilized in membranes of cells subjected to triple precipitation. Significant differences in the order parameter S, which characterizes the spectrum of the 5-doxylstearate probe, for samples incubated with the buffer and oxidized low-density lipoproteins were found. The dependence of the parameter on incubation time and the extend of oxidation of low-density lipoproteins were obtained. No significant differences in rotational correlation time of 16-doxylstearate between platelets incubated with and without oxidized low-density lipoproteins was observed within the limits of experimental error; however, the changes in the half-width of the low-field component may be considered reliable. The interaction of oxidized low-density lipoproteins with platelets leads to an increase in plasma membrane fluidity, thereby mediating the activating action on platelets.
Research Institute for Physicochemical Medicine, Ministry of Health and Medical Industry, Moscow, Russia.
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Vlasova
D001792:Blood Platelets / Q000737:chemistry / Q000648:ultrastructure*; D002462:Cell Membrane / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D010084:Oxidation-Reduction; D013113:Spin Labels
D008077:Lipoproteins, LDL; D013113:Spin Labels; C099252:oxidized low density lipoprotein
Vliianie okislennykh lipoproteinov nizkoĭ plotnosti na strukturu plazmaticheskoĭ membrany trombotsitov. Issledovanie metodom élektronnogo paramagnitnogo rezonansa.