[Prenatal diagnosis of viral infections. A two year study in Strasbourg].
We report here the results of a 2-year study on the prenatal diagnosis of viral infections in Strasbourg. This screening was carried out by virus isolation, by PCR assay, or by detection of IgM fetal antibody for 98 pregnant women at risk of transmitting one of the viruses that causes fetal disease such as parvovirus B19 (B19), Herpesviruses [cytomegalovirus (CMV), varicella-zoster virus, herpes simplex virus] and rubella virus. A viral etiology was proven in 7 out 98 cases: PCR applied to B19 DNA detection was positive in 5 amniotic fluids (AF), 2 fetal serums and one ascitic liquid. The diagnosis of 2 cases of CMV infection was obtained by both PCR and virus isolation in AF from twins fetuses. The detection of specific IgM in maternal serum or fetal serum is useful to achieve the diagnosis but serological tests on other samples have no efficiency. No virus was found in any other specimen, but the genome of Toxoplasma gondii was detected by PCR in 1 of 17 AF samples analyzed at the Institut de Parasitologie. These findings show that PCR assay is a sensitive method for the positive diagnosis of intrauterine infection and promises to careful follow-up of the pregnancy.
Institut de Virologie de la Faculté de Médecine, Hôpitaux Universitaires de Strasbourg, France.
N
N
Labouret
A
A
Cecille
M J
MJ
Wendling
S
S
Fritsch
J P
JP
Gut
F
F
Stoll-Keller
D000293:Adolescent; D000328:Adult; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D005260:Female; D005602:France / Q000453:epidemiology; D006561:Herpes Simplex / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D006562:Herpes Zoster / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control*; D008403:Mass Screening; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology* / Q000821:virology; D011296:Prenatal Diagnosis; D015203:Reproducibility of Results; D012307:Risk Factors; D012409:Rubella / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000517:prevention & control
Diagnostic prénatal des infections virales. Bilan de deux années d'activité à Strasbourg.