Bacterial biofilms and catheters in experimental urinary tract infection.
Employing in vitro and in vivo models of catheter-associated infection, biofilm formation of Pseudomonas aeruginosa in artificial urine and bactericidal activity of several classes alone or in combination with a macrolide antibiotic were investigated. In the course of continuous urine flow, bacteria adherent to a Teflon catheter formed microcolonies at 6 h and a biofilm at 24 h. Following 24 h of urine flow, the thickness and the density of the biofilm increased to about 20 microm and 10(8) cfu/mm3, respectively. Of the antibiotics examined, the fluoroquinolones showed the most potent bactericidal activity against the P. aeruginosa biofilms. However, those antibiotics were not always potentiated by the combination with a macrolide antibiotic.
Goto
T
T
Department of Urology, Faculty of Medicine, Kagoshima University, Japan.
Nakame
Y
Y
Nishida
M
M
Ohi
Y
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion; D018441:Biofilms; D024841:Fluoroquinolones; D018942:Macrolides; D011138:Polytetrafluoroethylene; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D014546:Urinary Catheterization; D014552:Urinary Tract Infections / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D018942:Macrolides; D011138:Polytetrafluoroethylene