High-performance liquid chromatography determination of residue levels on chicken carcasses treated with cetylpyridinium chloride.
Cetylpyridinium chloride (CPC) has been found to be effective in reducing contamination of chicken carcasses from a variety of microorganisms, including Escherichia coli O157:H7, Salmonella typhimurium, Campylobacter jejuni, Aeromonas hydrophila, Listeria monocytogenes, and Staphylococcus aureus. A procedure has been developed to determine residue levels on chicken carcasses after CPC treatment. For the analysis, chicken carcasses were extracted with 95% ethanol. The CPC concentration in the extract was measured by high-performance liquid chromatography (HPLC) with ultraviolet detection using dodecylpyridinium chloride (DPC) as an internal standard. The method was validated in the concentration range of 3-200 microg/ml CPC in ethanolic extract. This assay is rapid, precise, and accurate.
Zhou
X
X
Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Handie
A
A
Salari
H
H
Fifer
E K
EK
Breen
P J
PJ
Compadre
C M
CM
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000032:analysis*; D002594:Cetylpyridinium / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000891:Anti-Infective Agents, Local; D002594:Cetylpyridinium