Myofibrillar protein structure and assembly during idiopathic dilated cardiomyopathy.
A neutral protease, mekratin, active in human hearts at end stage idiopathic dilated cardiomyopathy (IDC), mediates the breakdown of cardiac myosin LC2. Myosin purified from IDC heart tissue forms unusually short synthetic thick filaments. Therefore, determination of filament length and mekratin distribution in IDC heart muscle were initiated. Native thick filaments were prepared directly from control and IDC tissues and analyzed. Also, paraffin-embedded tissue sections were stained with a fluorescently-labeled anti-protease antibody to establish its distribution in myocardial tissues. Control sections had only very weak, background levels of fluorescence whereas IDC sections stained intensely throughout, indicating a wide ranging distribution of the protease within the myocyte cytoplasm. SDS-PAGE revealed LC2 to be present in stoichiometric amounts in control but greatly reduced in IDC heart muscle. Native thick filaments from control myocardium were structurally stable. They had a median length of 1.65 microm with well-defined bare zones and displayed the 43 nm helical periodicity typical of the relaxed arrangement of myosin heads close to the filaments' shafts. In contrast, native IDC filaments were less stable, and had a median length of 0.9 microm. These filaments were highly disordered: they had no surface periodicity and myosin heads were positioned away from the filaments' shafts. The shorter, less stable, aperiodic thick filaments from IDC hearts appear to result from depletion of LC2 caused by increased activity of mekratin in the IDC myocardium.
Department of Neurobiology, MCP Hahnemann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, USA.
R J
RJ
Levine
J B
JB
Caulfield
P
P
Norton
P D
PD
Chantler
M R
MR
Deziel
H S
HS
Slayter
S S
SS
Margossian
D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D002311:Cardiomyopathy, Dilated / Q000201:enzymology / Q000378:metabolism*; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D009210:Myofibrils / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011487:Protein Conformation; D013194:Staining and Labeling
D009124:Muscle Proteins