A high-copy-number plasmid capable of replication in thermophilic cyanobacteria.
A 2.5 kb high-copy-number plasmid, pMA4 in thermophilic cyanobacterium Synechococcus sp. MA4 was isolated and characterized to develop a genetic engineering system for thermophilic cyanobacteria. The copy number of pMA4 was determined to be by densitometry about 350/cell. The pMA4 may be a type of rolling-circle plasmid, because a possible rep gene encoding 34 kD-protein and a consensus sequence of a double-stranded origin nick site of rolling circle plasmids were found in the pMA4 sequence. The pMA4 was electro-introduced into another thermophile, Synechococcus sp. MA19, which is the strongest poly-beta-hydroxybutyrate (PHB) accumulator in photoautotrophic organisms. The pMA4 was incorporated and retained in MA19. These results indicate that pMA4 could be developed as a useful vector for thermophilic cyanobacteria.
Miyake
M
M
National Institute of Bioscience and Human-Technology, Ministry of International Trade and Industry, Ibaraki, Japan.
Nagai
H
H
Shirai
M
M
Kurane
R
R
Asada
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism; D000458:Cyanobacteria / Q000254:growth & development*; D018274:Electroporation; D006885:Hydroxybutyrates / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry*; D011091:Polyesters / Q000378:metabolism; D000091402:Prohibitins; D017386:Sequence Homology, Amino Acid; D000094122:Polyhydroxybutyrates
D002245:Carbon Dioxide; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates