Putrescine and chlorpheniramine inhibit Ehrlich ascites tumor cell plasma membrane ferricyanide reductase activity.
The presence of putrescine or chlorpheniramine in the incubation medium of Ehrlich ascites tumor cells starved for 1 h significantly inhibits the rate of ferricyanide reduction by their plasma membrane redox system. Freshly harvested cells, without depletion of their intracellular pools of polyamines, and cells preincubated under conditions arranged to increase ornithine decarboxylase activity also reduced externally added ferricyanide at a lower rate than those cells starved for 1 h. All these data seems to indicate that the presence of putrescine is enough to significantly inhibit Ehrlich cell plasma membrane redox system activity.
Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Málaga, Spain.
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L
Rodríguez-Caso
F
F
Sánchez-Jiménez
M A
MA
Medina
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology* / Q000473:pathology; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002744:Chlorpheniramine / Q000494:pharmacology*; D005260:Female; D005292:Ferricyanides / Q000378:metabolism; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D011700:Putrescine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D005292:Ferricyanides; D006634:Histamine H1 Antagonists; C007931:hexacyanoferrate III; D002744:Chlorpheniramine; D009247:NADH, NADPH Oxidoreductases; C014227:ferricyanide reductase; D011700:Putrescine