Macromolecular crystal annealing: evaluation of techniques and variables.
Additional examples of successful application of macromolecular crystal annealing are presented. A qualitative evaluation of variables related to the annealing process was conducted using a variety of macromolecular crystals to determine in which cases parameters may be varied and in which cases the original macromolecular crystal annealing protocol is preferred. A hypothesis is presented relating the solvent content of the crystal to the specific protocol necessary for the successful application of annealing.
University of Tennessee/Oak Ridge, Graduate School of Biomedical Sciences, USA.
J M
JM
Harp
B L
BL
Hanson
D E
DE
Timm
G J
GJ
Bunick
D000818:Animals; D005069:Evaluation Studies as Topic; D011506:Proteins / Q000737:chemistry*
D011506:Proteins