Identification and estimation of endogenous digoxin in biological fluids and tissues by TLC and HPLC.
A procedure for estimation of digoxin in biological samples after adding a known quantity of digoxin followed by extraction, separation by TLC and HPLC is described. The identity of digoxin thus extracted from rat brain has been established by reaction with digoxin antibody and by its inhibition of Na(+)-K+ ATPase activity. The method could be a better substitute to the routine radioimmunoassay as interfering substances are removed by TLC and HPLC.
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.
P
P
Arun
A
A
Ravikumar
S
S
Leelamma
P A
PA
Kurup
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D001923:Brain Chemistry / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004077:Digoxin / Q000032:analysis* / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004077:Digoxin