Deletions below 10 megabasepairs are detected in comparative genomic hybridization by standard reference intervals.
Comparative genomic hybridization (CGH) is a widely used technique for studying chromosomal imbalances. The sensitivity of the technique is, however, relatively low. Deletions down to a size of 10-12 Mbp have been detected by the use of fixed diagnostic thresholds. In this study, we applied standard reference intervals as detection criteria on a number of deletions in the range of 3 Mbp to 14-18 Mbp. All deletions were detected. Thus, detection by standard reference intervals confers a considerably higher sensitivity to CGH analysis compared to fixed diagnostic thresholds. Genes Chromosomes Cancer 25:410-413, 1999.
Kirchhoff
M
M
Cytogenetic Laboratory, Department of Clinical Genetics, Juliane Marie Center, National University Hospital, Copenhagen, Denmark. markir@rh.dk
Gerdes
T
T
Maahr
J
J
Rose
H
H
Bentz
M
M
Döhner
H
H
Lundsteen
C
C
D002869:Chromosome Aberrations / Q000235:genetics; D025063:Chromosome Disorders; D004273:DNA, Neoplasm / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods; D012015:Reference Standards; D017384:Sequence Deletion
D004273:DNA, Neoplasm