PAC and cosmid contig spanning the HOXA cluster on human chromosome 7p15.
To construct the PAC and cosmid contig map spanning the HOXA cluster on human chromosome 7, we used 9 DNA markers (D7S2243, D7S3010, HOXA1, EVX1, 750, pBH8, p60, p8.0, and HOXA11), among which the final 4 were generated in this study by shotgun cloning strategy. From the libraries, 5 PAC and 35 cosmid clones were screened and as a result, an overlapping continuous array of cosmid and PAC clones covering the genomic region (about 200 kb) spanning the entire cluster were constructed. The isolated cosmids contained several consecutive HOX genes of regional group, probably sharing the regulatory processes such as alternative splicing or polyadenylation, and thus could be used as useful materials for elucidating the molecular mechanism of HOX gene expression in the future.
Dept. of Anatomy, Yonsei University College of Medicine, Seoul, Korea. mhkim1@yumc.yonsei.ac.kr
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Park
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Chung
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D017101:Bacteriophage P1 / Q000235:genetics; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D003001:Cloning, Molecular; D020451:Contig Mapping; D003360:Cosmids / Q000235:genetics; D005801:Genes, Homeobox / Q000235:genetics; D005819:Genetic Markers; D018398:Homeodomain Proteins; D006801:Humans; D005810:Multigene Family; D015534:Trans-Activators / Q000235:genetics*
D005819:Genetic Markers; D018398:Homeodomain Proteins; D015534:Trans-Activators; C088144:HoxA protein