Anti-allergic actions of the leaves of Castanea crenata and isolation of an active component responsible for the inhibition of mast cell degranulation.
The anti-allergic actions of the leaves of Castanea crenata (Fagaceae) were studied. The water extract demonstrated potent anti-allergic actions in in vivo and in vitro experiments. The oral or intraperitoneal administration of the extract (100 or 200 mg/kg) caused a significant inhibition of the 48 hr-PCA (up to 90%) and the vascular permeability induced by histamine or serotonin in rats (about 80%). The anaphylactic release of beta-hexosaminidase from RBL-2H3 cells was also significantly inhibited by the extract in a dose-dependent manner with an IC50 value of 230 microg/ml. The activity-guided fractionation of the extract, based on the determination of inhibitory effect upon the release of beta-hexosaminidase, led to the isolation of quercetin as an active principle responsible for the inhibition of degranulation.
Lee
E
E
Pharmacology Laboratory, College of Pharmacy, Chonnam National University, Kwang-Ju, Korea.
Choi
E J
EJ
Cheong
H
H
Kim
Y R
YR
Ryu
S Y
SY
Kim
K M
KM
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects*; D015550:Cell Degranulation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000009:adverse effects; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D010537:Peritoneum / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011794:Quercetin / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000009:adverse effects; D013997:Time Factors; D014867:Water / Q000737:chemistry; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
D018926:Anti-Allergic Agents; D010936:Plant Extracts; D014867:Water; D012701:Serotonin; D006632:Histamine; D011794:Quercetin; D001619:beta-N-Acetylhexosaminidases