Detection of tumor promoters by early antigen expression of EB virus in Raji cells using a fluorescence microplate-reader.
As an in vitro assay for possible tumor promoters, we designed a quantitative immunofluorometric method to detect Epstein-Barr virus early antigen (EBV-EA) expression in Raji cells. In this method, anti-EBV-EA monoclonal antibody, a fluorogenic substrate and a fluorescence microtiterplate-reader were employed. 12-O-Tetradecanoylphorbol-13-acetate (TPA) was shown to be a potent inducer. EBV-EA induction by TPA was related to the activation of protein kinase C and phospholipase A2. The chemicals that reacted positively were okadaic acid, diethylstilbestrol, progesterone, sodium phenobarbital, aldrin and dieldrin. Lithocholic acid, testosterone and DDT were equivocal in the present experiments. Eight other chemicals tested did not react.
Ohmori
K
K
Department of Food and Drug Science, Kanagawa Prefectural Public Health Laboratories, Yokohama, Japan.
Miyazaki
K
K
Umeda
M
M
D000098:Acetophenones / Q000494:pharmacology; D000956:Antigens, Viral / Q000096:biosynthesis / Q000187:drug effects*; D002273:Carcinogens / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005470:Fluorometry; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D010172:Palmitoylcarnitine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000821:virology; D014775:Virus Activation / Q000187:drug effects
D000098:Acetophenones; D000956:Antigens, Viral; D002273:Carcinogens; D004791:Enzyme Inhibitors; D010172:Palmitoylcarnitine; D013755:Tetradecanoylphorbol Acetate; C006821:4-bromophenacyl bromide