Comparison of CMV, RSV, SV40 viral and Vlambda1 cellular promoters in B and T lymphoid and non-lymphoid cell lines.
Determining the activity of viral and cellular regulatory elements in B or T lymphoid cell lines would facilitate appropriate utilization of the regulatory sequences for gene transfer- and expression-dependent applications. We have compared the activity of the CMV, RSV and SV40 viral promoter/enhancers as well as the Vlambda1 cellular promoter, in three B cell lines (REH, SMS-SB, C3P), three T cell lines (CEM, Jurkat, ST-F10), and two non-lymphoid cell lines (K-562, HeLa) using the luciferase reporter gene. In B cell lines, the activity of the CMV promoter/enhancer construct was the highest ranging from 10- to 113-fold greater than that of SV40. In contrast, in T cell lines the RSV promoter/enhancer activity was 11-65-fold higher than that of SV40. The Vlambda1 promoter activity was close to that of SV40 promoter/enhancer in most of the cell lines tested. We conclude that CMV and RSV promoter/enhancers contain stronger regulatory elements than do the SV40 and Vlambda1 for expression of genes in lymphoid cell lines.
Department of Immunology, University of Toronto, Toronto, Canada.
A A
AA
Zarrin
L
L
Malkin
I
I
Fong
K D
KD
Luk
A
A
Ghose
N L
NL
Berinstein
D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*; D013601:T-Lymphocytes / Q000821:virology*; D014407:Tumor Cells, Cultured