Perchloric acid-soluble proteins from goat liver inhibit chemical carcinogenesis of Syrian hamster cheek-pouch carcinoma.
Chemically induced Syrian hamster cheek-pouch squamous cell carcinoma is very similar to the corresponding human tumour. This paper describes a blind study in which inhibition of dimethylbenzanthracene-induced cheek-pouch tumours by a goat liver extract denominated UK101 was investigated. Less than 40% of animals treated with UK101 developed tumours compared with 100% of the controls. Intermediate results (80%) were noted in a positive control group treated with Calmette-Guerin bacillus. Immunocytochemical testing of cheek-pouch mucosa by Mib5 showed significantly less proliferating cells in UK101 animals than in the controls. The effect of UK101 was completely reversed when dexamethasone was added in a third control group. A significant difference in complement-mediated cytotoxicity was noted in the sera of UK101-tested and control animals. These findings suggest that an immune mechanism is responsible for the inhibition of hamster cheek-pouch carcinoma by UK101.
Ghezzo
F
F
Department of Biological Science, University of Turin, Italy.
Berta
G N
GN
Bussolati
B
B
Bosio
A
A
Corvetti
G
G
Di Carlo
F
F
Bussolati
G
G
Guglielmone
R
R
Bartorelli
A
A
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002610:Cheek; D006224:Cricetinae; D003907:Dexamethasone / Q000494:pharmacology; D006041:Goats; D008099:Liver / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D010472:Perchlorates / Q000737:chemistry*; D011506:Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility
D002273:Carcinogens; D010472:Perchlorates; D011506:Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene; D003907:Dexamethasone