The use of biosensor technology for the engineering of antibodies and enzymes.
Recently developed scientific instrumentation featuring surface plasmon resonance detection allows the detection of biomolecular interactions in real time and without chemical modification of the binding partners. These biosensors are proving invaluable tools in protein engineering, particularly in research aimed at the isolation and improvement of protein binders and catalysts from macromolecular repertoires containing billions of individual members. This article reviews the use of biosensor technology for the isolation and characterization of engineered antibodies and enzymes.
Institute of Molecular Biology and Biophysics, Swiss Federal Institute of Technology CH-8093 Zurich, Switzerland.
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Huber
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Demartis
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Neri
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation; D002384:Catalysis; D015195:Drug Design; D004798:Enzymes / Q000737:chemistry* / Q000302:isolation & purification; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005285:Fermentation; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D011487:Protein Conformation; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D013816:Thermodynamics
D000906:Antibodies; D004798:Enzymes; D000939:Epitopes; D011994:Recombinant Proteins