Effect of smoking one cigarette on antioxidant metabolites in the saliva of healthy smokers.
Concentrations of glutathione, uric acid and total antioxidant activity, expressed as Trolox (a water-soluble vitamin E analogue) equivalent, were measured in the saliva of healthy non-smokers and smokers before and just after smoking a single cigarette. There was no statistically significant difference between smokers and non-smokers in uric acid concentrations and total radical-trapping antioxidant capacity, but glutathione concentrations were significantly (p < 0.05) higher in smokers. Smoking of a single cigarette induced a significant reduction in glutathione concentration (p < 0.05). Salivary antioxidant power may affect individual sensitivity toward tobacco stress.
Istituto di Chimica e Chimica Clinica, CNR Centro Chimica dei Recettori, Università Cattolica del Sacro Cuore, Rome, Italy.
B
B
Zappacosta
S
S
Persichilli
P
P
De Sole
A
A
Mordente
B
B
Giardina
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D052160:Benzothiazoles; D002851:Chromatography, High Pressure Liquid; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000378:metabolism; D012463:Saliva / Q000378:metabolism*; D012907:Smoking / Q000378:metabolism*; D013451:Sulfonic Acids; D014527:Uric Acid / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D013451:Sulfonic Acids; D014527:Uric Acid; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009195:Peroxidase; D005978:Glutathione