Selective activation of phospholipase D2 by unsaturated fatty acid.
Although oleate has been implicated in the regulation of phospholipase D (PLD) activity, the molecular identity of the oleate-stimulated PLD is still poorly understood. We now report that oleate selectively stimulates the enzymatic activity of PLD2 but not of PLD1, with an optimal concentration of 20 microM in vitro. Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP2) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 microM. These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP2.
Kim
J H
JH
Department of Life Science and School of Environmental Engineering, Pohang University of Science and Technology, South Korea.
Kim
Y
Y
Lee
S D
SD
Lopez
I
I
Arnold
R S
RS
Lambeth
J D
JD
Suh
P G
PG
Ryu
S H
SH
D004305:Dose-Response Relationship, Drug; D006801:Humans; D019301:Oleic Acid / Q000494:pharmacology*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D020298:U937 Cells
D019269:Phosphatidylinositol 4,5-Diphosphate; D019301:Oleic Acid; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1