Equinatoxins, pore-forming proteins from the sea anemone Actinia equina, belong to a multigene family.
The multigene family of equinatoxins, pore-forming proteins from sea anemone Actinia equina, has been studied at the protein and gene levels. We report the cDNA sequence of a new, sphingomyelin inhibited equinatoxin, EqtIV. The N-terminal sequences of natural Eqt I and III were also determined, confirming two isoforms of EqtI, differing at position 13. The number of Eqt genes determined by Southern blot hybridization was found to be more than five, indicating that Eqts belong to a multigene family.
Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia. gregor.anderluh@uni-lj.si
G
G
Anderluh
I
I
Krizaj
B
B
Strukelj
F
F
Gubensek
P
P
Macek
J
J
Pungercar
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003064:Cnidarian Venoms / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D005810:Multigene Family; D020033:Protein Isoforms / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D012615:Sea Anemones / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D003064:Cnidarian Venoms; D018076:DNA, Complementary; D020033:Protein Isoforms; D011994:Recombinant Proteins; C012307:equinatoxin