Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse.
The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 x 10(9) or 5 x 10(9) plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 x 10(9) plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.
Introgen Therapeutics Inc., Houston, Texas 77054, USA.
M
M
Wood
P
P
Perrotte
E
E
Onishi
M E
ME
Harper
C
C
Dinney
L
L
Pagliaro
D R
DR
Wilson
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008156:Luciferases / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000201:enzymology
D008156:Luciferases