Induction of resistance against Schistosoma mansoni infection by passive transfer of an IgG2a monoclonal antibody.
Monoclonal antibodies can confer resistance to schistosome infections. This has led to identification of several protective antigens. An IgG2a monoclonal antibody designated BRL4 mAb identified a 74-kDa antigen in antigenic extract of Schistosoma mansoni adult worms. The target antigen was localized in gut and tegument. In 3 passive transfer experiments, the BRL4 mAb conferred 51.6, 41.9 and 53.8% protection levels into female Swiss mice. Histopathological examination revealed a marked decrease in number, size, collagen and reticular fibers of the liver granulomas. Further experiments using purified 74-kDa-target antigen as a candidate vaccine will be performed.
Attallah
A M
AM
Biotechnology Research Center, New Damietta City, Egypt.
Attia
H
H
El-Nashar
E M
EM
Nawar
A
A
Abdel Kader
K
K
Ismail
H
H
El Ebeidy
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism / Q000627:therapeutic use; D000947:Antigens, Helminth / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism / Q000627:therapeutic use; D008099:Liver / Q000473:pathology; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007074:Immunoglobulin G