Evidence that constitutively active luteinizing hormone/human chorionic gonadotropin receptors are rapidly internalized.
The luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, which belongs to the family of G-protein coupled receptors, plays an important role in gonadal steroidogenesis. Substitution of aspartic acid 556 of the LH/hCG receptor with glycine (D556G) creates a constitutively active receptor that activates adenylyl cyclase in the absence of hormone. To examine receptor internalization, human embryonic kidney cells (293 T) expressing wild type (WT) or D556G mutant receptors were incubated with [125I]hCG and subsequently analyzed for cell surface bound and internalized radioactivity. Comparison of the rate constants of internalization of the D556G mutant and WT receptors revealed that the rate of internalization of the D556G mutant was five times greater than that of the WT receptor. Although the D556G receptor internalizes [125I]hCG rapidly, a corresponding increase in [125I]hCG degradation was not seen. The internalization of another constitutively active LH/hCG receptor (aspartic acid 556 to tyrosine) was also greater than that of the WT receptor. Internalization of receptor bound [125I]hCG was inhibited by a hypertonic sucrose solution, confirming that the ligand enters the cell by receptor-mediated endocytosis. Furthermore, the constitutively active D556G and D556Y LH/hCG receptors utilize the arrestin dependent internalization pathway. These results suggest that the active state conformation of the constitutively active receptor is conducive to rapid internalization.
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0617, USA.
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Bradbury
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Menon
D019390:Arrestins / Q000494:pharmacology; D001224:Aspartic Acid / Q000235:genetics; D001665:Binding Sites / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000037:antagonists & inhibitors / Q000378:metabolism; D005998:Glycine / Q000235:genetics; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D014443:Tyrosine / Q000235:genetics
D019390:Arrestins; D006063:Chorionic Gonadotropin; D007457:Iodine Radioisotopes; D011974:Receptors, LH; D001224:Aspartic Acid; D014443:Tyrosine; D005998:Glycine