The mitochondrial Pylaiella littoralis nad11 gene contains only the N-terminal FeS-binding domain.
We describe a nad11 gene located on the mitochondrial genome of the brown alga Pylaiella littoralis. This gene is cotranscribed with other neighbouring nad genes. It encodes the first domain only of the Nad11 polypeptide, i.e. a 23-kDa, FeS-binding domain instead of the usual 75/80-kDa protein found in the mitochondrial or alpha-proteobacterial complex I enzymes. The second domain of the protein, of unknown function, seems to be entirely missing in this alga. Cyanobacteria, beta-proteobacteria and actinomycetes also feature small homologous genes, known as hoxU, and it has been suggested that these could function in complex I of cyanobacteria. These observations indicate that complex I can probably function with the first domain only of the 75-kDa protein. P. littoralis represents the first such example within the alpha-proteobacterial/mitochondrial lineage.
Station Biologique de Roscoff, CNRS (UMR 1931), B.P. 74, 29682, Roscoff Cedex, France.
M P
MP
Oudot
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Kloareg
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Loiseaux-de Goër
D020418:Algal Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D005796:Genes; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000459:Phaeophyceae / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013455:Sulfur / Q000378:metabolism*
D020418:Algal Proteins; D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D013455:Sulfur; D007501:Iron