DLX-7 homeobox gene regulates c-myc and GATA-1 gene expression in different stages respectively.
Homeobox genes code for transcription factors with a highly conserved DNA binding motif, but their target genes are largely unknown. We have shown that inhibition of the DLX-7 homeobox gene by an antisense oligonucleotide decreased the expression of c-myc and GATA-1 genes. Nuclear run-on assay revealed decreased transcriptional activity of c-myc but not of GATA-1 after DLX-7 antisense treatment. Actinomycin D chase experiments demonstrated that GATA-1 mRNA became unstable as a result of DLX-7 inhibition, whereas c-myc mRNA stability was unaffected. These results suggest that DLX-7 may regulate c-myc and GATA-1 genes at transcriptional and post-transcriptional levels.
First Department of Internal Medicine, Tokyo Medical University, Shinjuku-ku, Tokyo 160-0023, Japan.
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Shimamoto
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Ohyashiki
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Takeshita
D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D015536:Down-Regulation; D016193:G1 Phase / Q000187:drug effects; D005786:Gene Expression Regulation / Q000502:physiology*; D005801:Genes, Homeobox; D016259:Genes, myc; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D016376:Oligonucleotides, Antisense; D014157:Transcription Factors; D003609:Dactinomycin