[The use of microfluorimetric analysis for the studies of the influence of changed gravity on isolated lymphocytes in the spleen of the mouse].
Effects of simulated changed gravity on isolated splenetic lymphocytes of mouse were evaluated with the microfluorimetic technique enabling observation of probe fluorescence in individual cells. It was stated that 5 and 60 min of clinostatting stimulated fluorochrome accumulation in cells whereas centrifugation, particularly for 60-minutes, decreased the ability of cells to accumulate fluorescein. Also, alteration of the gravity force had the opposite effect on the ability of cells to retain fluorescein. The most significant drain of the dye immediately after short hypogravity may be indicative of the functional lability of cells during clinostatting. However, as judged by slight changes in intracellular pH, metabolic and regulatory functions were not affected. Hypergravity for 15 min decreased intracellular pH. The increased period of centrifugation stabilized the parameter. Absence of changes in the proliferative activity in all the test exposures also backs up the conclusion that short-term changes in gravity do not produce any substantial shifts in the morphofunctional state of cells in vitro.
Turovetskiĭ
V B
VB
Andreev
A I
AI
Buravkova
L B
LB
Rubin
A B
AB
D000818:Animals; D019793:Fluorescein / Q000378:metabolism; D005470:Fluorometry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D018471:Hypergravity; D018472:Hypogravity; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D013154:Spleen / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
D019793:Fluorescein
Ispol'zovanie mikrofluorimetricheskogo analiza dlia izucheniia vliianiia izmenennoĭ sily tiazhesti na izolirovannye limfotsity selezenki mysheĭ.