Molecular cloning and characterization of MSRV-related sequences associated with retrovirus-like particles.
New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas the env sequence is closer to type D. A tryptophan-like (W) tRNA primer-binding site was identified downstream of the RU5 region in the 5'LTR, and the U3R region cloned in the 3'LTR exhibited potent promoter activity. MSRV clones define a novel family of endogenous elements, HERV-W. From our data, HERV-W RNAs are copackaged in extracellular particles which might be produced by replication-competent or transcomplemented HERV-W copies or by an exogenous member of the HERV-W family.
Ecole Normale Supérieure de Lyon, UMR 103 CNRS-bioMérieux, 46, Allée d'Italie, Lyon Cedex 07, 69364, France.
F
F
Komurian-Pradel
G
G
Paranhos-Baccala
F
F
Bedin
A
A
Ounanian-Paraz
M
M
Sodoyer
C
C
Ott
A
A
Rajoharison
E
E
Garcia
F
F
Mallet
B
B
Mandrand
H
H
Perron
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003001:Cloning, Molecular; D018388:Codon, Terminator; D020077:Endogenous Retroviruses / Q000235:genetics*; D015686:Gene Products, env / Q000378:metabolism; D006801:Humans; D019426:Integrases / Q000378:metabolism; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000821:virology*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
D018388:Codon, Terminator; D015686:Gene Products, env; D019426:Integrases