Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour fluorescence in situ hybridization.
Double colour fluorescence in situ hybridization with sex chromosome probes was applied on sperm cells of five Swedish Holstein-Friesian bulls. It was demonstrated that cosmids with strong fluorescence signals and scraped chromosomes can successfully be used as markers in this type of study. X and Y segregated as expected according to a 1:1 ratio, and there were no interindividual variations. There was a tendency for there to be more Y- than X-bearing spermatozoa, but this bias was assumed to be due to the markers used. Disomic spermatozoa occurred with a frequency of more than 0.1 % (0.067% XX, 0.029% YY, and 0.029% XY), which is considerably lower than the frequency in humans. Diploid sperm cells occurred with a frequency of 0.05 %.
Hassanane
M
M
Centre of Reproductive Biology, Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Kovacs
A
A
Laurent
P
P
Lindblad
K
K
Gustavsson
I
I
D000782:Aneuploidy; D000818:Animals; D002417:Cattle; D017404:In Situ Hybridization, Fluorescence / Q000379:methods / Q000662:veterinary*; D008297:Male; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D013094:Spermatozoa / Q000648:ultrastructure*; D014960:X Chromosome / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
D015335:Molecular Probes