Time course tissue distribution of infectious salmon anaemia virus in experimentally infected Atlantic salmon Salmo salar.
Atlantic salmon Salmo salar L. were injected intraperitoneally with infectious salmon anaemia virus (ISAV)-infective tissue homogenate to clarify the tissue distribution of ISAV in a time course study. Fish were sampled at 11 different intervals between 1 and 40 d post-infection (p.i.) and mid-kidney, head kidney, liver, spleen, intestine, gills, muscle and heart were tested for the presence of ISAV by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that during a disease outbreak, ISAV is present in most organs. It was possible to detect ISAV at all sampling times in at least 1 of the fish examined. However, for the first 8 d p.i. positive RT-PCR results were predominantly found in samples from the head kidney and mid-kidney. Fish giving positive samples after Day 13 p.i. were RT-PCR positive in most organs. These results indicated that between Days 8 to 13 p.i. considerable replication of the virus occurred, combined with wide tissue dissemination.
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo, Norway. espen.rimstad@veths.no
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Rimstad
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Falk
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Mikalsen
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Teig
D000740:Anemia / Q000662:veterinary* / Q000821:virology; D000818:Animals; D005393:Fish Diseases / Q000821:virology*; D005880:Gills / Q000821:virology; D006321:Heart / Q000821:virology; D007422:Intestines / Q000821:virology; D007668:Kidney / Q000821:virology; D008099:Liver / Q000821:virology; D009132:Muscles / Q000821:virology; D009975:Orthomyxoviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012474:Salmon; D013154:Spleen / Q000821:virology; D013997:Time Factors
D012367:RNA, Viral