PHA production, from bacteria to plants.
The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB; and PHA synthase, phaC) were engineered for plant plastid targeting and expressed using leaf (e35S) or seed-specific (7s or lesquerella hydroxylase) promoters in Arabidopsis and Brassica. PHA yields in homozygous transformants were 12-13% of the dry mass in homozygous Arabidopsis plants and approximately 7% of the seed weight in seeds from heterozygous canola plants. When a threonine deaminase was expressed in addition to bktB, phaB and phaC, a copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate was produced in both Arabidopsis and Brassica.
Valentin
H E
HE
Monsanto Company, Agricultural Sector, St. Louis, MO 63198, USA.
Broyles
D L
DL
Casagrande
L A
LA
Colburn
S M
SM
Creely
W L
WL
DeLaquil
P A
PA
Felton
H M
HM
Gonzalez
K A
KA
Houmiel
K L
KL
Lutke
K
K
Mahadeo
D A
DA
Mitsky
T A
TA
Padgette
S R
SR
Reiser
S E
SE
Slater
S
S
Stark
D M
DM
Stock
R T
RT
Stone
D A
DA
Taylor
N B
NB
Thorne
G M
GM
Tran
M
M
Gruys
K J
KJ
D000102:Acetyl-CoA C-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D020118:Cupriavidus necator / Q000201:enzymology* / Q000235:genetics; D006720:Homozygote; D008958:Models, Molecular; D015394:Molecular Structure; D018515:Plant Leaves; D010944:Plants / Q000378:metabolism; D030821:Plants, Genetically Modified; D011091:Polyesters / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012639:Seeds
D011091:Polyesters; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C023744:acetoacetyl-CoA reductase; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase; D000102:Acetyl-CoA C-Acyltransferase