Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases.
A screen for proteins that interact with beta 2-syntrophin led to the isolation of MAST205 (microtubule-associated serine/threonine kinase-205 kD) and a newly identified homologue, SAST (syntrophin-associated serine/threonine kinase). Binding studies showed that beta 2-syntrophin and MAST205/SAST associated via a PDZ-PDZ domain interaction. MAST205 colocalized with beta 2-syntrophin and utrophin at neuromuscular junctions. SAST colocalized with syntrophin in cerebral vasculature, spermatic acrosomes and neuronal processes. SAST and syntrophin were highly associated with purified microtubules and microtubule-associated proteins, whereas utrophin and dystrophin were only partially associated with microtubules. Our data suggest that MAST205 and SAST link the dystrophin/utrophin network with microtubule filaments via the syntrophins.
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618, USA.
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Lumeng
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Phelps
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Crawford
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PD
Walden
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Barald
J S
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Chamberlain
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D049029:Dystrophin-Associated Proteins; D006624:Hippocampus / Q000201:enzymology; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D009474:Neurons / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017966:Pyramidal Cells / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology
D049029:Dystrophin-Associated Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C084505:Mtssk protein, mouse; D011994:Recombinant Proteins; C091824:syntrophin; D017346:Protein Serine-Threonine Kinases