Vaccine potential of 56-66 kDa protease secreted by Entamoeba histolytica.
Excretory/secretory (ES) antigens and sub-cellular fractions of E. histolytica (HM1:IMSS strain) were tested for the presence of common proteases using substrate gel electrophoresis. We obtained two E. histolytica proteases (56-66 kDa and 29 kDa) from ES material, soluble components and plasma membrane. Protease 56-66 kDa from ES antigen was selected for immunizing hamsters because it gave a consistent broad band. We observed 62.5 per cent protection in immunized animals, compared to 0 per cent in unimmunized controls. Although all vaccinated golden hamsters showed high antibody response, there was no correlation between antibody titres and protection. 56-66 kDa ES protease could thus prevent disease and could be a candidate molecular vaccine against amoebiasis.
Gupta
S
S
Department of Gastroenterology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow.
Naik
S
S
Naik
S R
SR
D000562:Amebiasis / Q000517:prevention & control*; D000656:Amoeba / Q000201:enzymology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D006224:Cricetinae; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use*; D007114:Immunization; D008647:Mesocricetus; D008970:Molecular Weight; D016052:Protozoan Vaccines / Q000737:chemistry / Q000627:therapeutic use*
D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D010450:Endopeptidases