The effects of polyols on the thermal stability of calf thymus DNA.
The effects on thermal denaturation of calf thymus DNA (ct-DNA) and its conformational changes induced by the presence in solution of different polyols, namely glycerol, i-erytritol, L( -- ) and D( + ) arabitol, D-mannitol, D-sorbitol and myo-inositol, have been investigated by means of differential scanning calorimetry (DSC) and circular dichroism (CD). By increasing the concentration of these additives a decrease in both the denaturation enthalpy (deltadH) and temperature of the maximum of the denaturation peak (Tmax) of DNA is observed. The values of these thermodynamic parameters depend on both the nature and concentration of the solute. The overall destabilization of DNA molecule has been related to the different capability of polyhydric alcohols to interact with the polynucleotide solvation sites replacing water and to the modification of the electrostatic interactions between the polynucleotide and its surrounding atmosphere of counterions. The particular behaviour of L( -- ) arabitol, which showed a much greater destabilizing ability compared to the other polyols, was further investigated and attributed to a direct more effective interaction with the double helix of DNA. CD spectra showed only a slight alteration of DNA-B structure in the presence of all the molecules here studied, except for L( -- ) arabitol where the DNA molecule seems to undergo a meaningful conformational change. The salt concentration dependence of DNA thermal stability in the presence of L( -- ) arabitol indicates a conformational change of polynucleotide towards a more extended conformation.
Department of Chemistry, University Federico II of Naples, Italy.
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D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000187:drug effects*; D004896:Erythritol / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007294:Inositol / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D012492:Salts; D013012:Sorbitol / Q000494:pharmacology; D013402:Sugar Alcohols / Q000494:pharmacology*; D013696:Temperature; D013816:Thermodynamics; D013950:Thymus Gland / Q000737:chemistry*
D012492:Salts; D013402:Sugar Alcohols; D008353:Mannitol; D007294:Inositol; D013012:Sorbitol; D004247:DNA; D005990:Glycerol; D004896:Erythritol; C014999:arabitol