Isolation of a species-specific satellite DNA with a novel CENP-B-like box from the North African rodent Lemniscomys barbarus.
A species-specific satellite DNA (Lb-MspISAT) was isolated from the North African rodent Lemniscomys barbarus. This DNA is highly homogeneous in the sequence of different repeats and shows no internal repetitions. Filter and in situ hybridizations demonstrated that it is tandemly repeated at the centromeres of all chromosomes of the complement. A 19-bp CENP-B-like motif was found in Lb-MspISAT which conserves 12 of the 17-bp of the human CENP-B box, but only 5 of the 9-bp of the canonical sequence that is necessary to bind the CENP-B protein. Compared with the human CENP-B box, nucleotide substitutions and insertions increase the palindromic structure of this motif. The possibilities that it may be involved in centromeric function or in homogenization of the Lb-MspISAT sequence are discussed.
Stitou
S
S
Facultad de Ciencias, Universidad de Granada, Granada, 18071, Spain.
Díaz de la Guardia
R
R
Jiménez
R
R
Burgos
M
M
D000352:Africa, Northern; D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere / Q000235:genetics; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D004268:DNA-Binding Proteins; D005260:Female; D006570:Heterochromatin / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D013045:Species Specificity
D001324:Autoantigens; C497926:CENPB protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D006570:Heterochromatin