Modulation of accelerated repopulation in mouse skin during daily irradiation.
OBJECTIVE
The timing of acceleration of repopulation in the epidermis during daily irradiation is related to the development of skin erythema and epidermal hypoplasia. Therefore, the relationship between impairment of the epidermal barrier function, the dermal inflammatory response and epidermal hypoplasia with the acceleration of repopulation was investigated. MATERIALS AND PURPOSE: Skin fields of approximately 1 cm2 on the thighs of TUC mice were given five daily fractions of 3 Gy in each week followed by top-up doses at the end of the first, the second, or the third week to determine residual epidermal tolerance and to calculate repopulation rates in weeks 1, 2, or 3. Systemic modulation of repopulation was attempted by daily indomethacine during fractionated irradiation whereas tape stripping or UV-B exposure before the start of fractionated irradiation attempted local modulation. In parallel experiments, the water permeability coefficient of the epidermis was determined ex vivo by studying transepidermal transport of tritiated water.


RESULTS
Without modulation, no repopulation was found in the first week of daily fractionation but repopulation compensated 30% of the dose given in week two and 70% of the dose given in week three. Only tape stripping before the start of fractionated irradiation accelerated repopulation in week one. UV-B had no effect on repopulation although it stimulated proliferation as much as tape stripping. Indomethacin did not suppress acceleration of repopulation. A significant increase in transepidermal water loss was found but only after repopulation had already accelerated.


CONCLUSIONS
Acceleration of repopulation in mouse epidermis during daily-fractionated irradiation is not related to the simultaneous development of an inflammatory response. Also, the loss of the epidermal barrier function is not involved in the development of the acceleration response, which rather seems to be triggered directly by the decreased cellularity of the epidermis.
Trott
K R
KR
Department of Radiation Biology, St. Bartholomew's Hospital, London, UK.
Shirazi
A
A
Heasman
F
F
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D003872:Dermatitis / Q000209:etiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004890:Erythema / Q000209:etiology; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000209:etiology; D011835:Radiation Protection; D019275:Radiopharmaceuticals; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors; D014316:Tritium; D014466:Ultraviolet Rays; D014867:Water / Q000378:metabolism; D014870:Water Loss, Insensible / Q000187:drug effects / Q000528:radiation effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D019275:Radiopharmaceuticals; D014867:Water; D014316:Tritium; D007213:Indomethacin