Regions of the pea Lhcb1*4 promoter necessary for blue-light regulation in transgenic Arabidopsis.
Pea (Pisum sativum) and Arabidopsis contain similar, if not identical, blue-light (BL)-responsive systems that alter expression of specific members of the Lhcb (light-harvesting chlorophyll-binding) gene family. In both plants a single, short pulse of low-fluence BL (threshold = 10(-1) micromol m-2) causes an increase in the rate of transcription from specific members of the Lhcb gene family in etiolated seedlings. Constructs of the BL-regulated pea Lhcb1*4 promoter (PsLhcb1*4) were created, which altered sequences previously implicated in light responses, deleted the 5'-promoter sequence, or removed the 5'-untranslated region. These constructs were tested for BL induction in transgenic Arabidopsis. The PsLhcb1*4 promoter deletions to -150 bp maintained normal fluence response, time course, and reciprocity characteristics. The 5'- untranslated region contained enhancer elements, but was not necessary for BL induction. The -95 to +2 promoter was capable of responding to BL, whereas sequences from -50 were not. Promoters that lack conserved light-regulatory elements or sequences directly implicated in phytochrome and circadian responses retained BL activity, suggesting that the low-fluence BL response utilizes regions of the promoter independent of those that modulate the phytochrome and circadian responses.
Folta
K M
KM
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
Kaufman
L S
LS
D020121:5' Untranslated Regions; D017360:Arabidopsis / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D001483:Base Sequence; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D018532:Pisum sativum / Q000235:genetics* / Q000528:radiation effects; D010750:Phosphoproteins / Q000235:genetics; D010834:Phytochrome / Q000096:biosynthesis; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D020121:5' Untranslated Regions; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010750:Phosphoproteins; D010834:Phytochrome; C103646:NPH1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases