Transient effects of nerve injury on estimates of sensory neuron number in juvenile bullfrog.
The effect of lumbar spinal nerve (SN) transection on estimates of neuron number was investigated in the dorsal root ganglia (DRGs) of juvenile bullfrogs (Rana catesbeiana). SN8 and SN10 were transected on one side, and SN9 was left intact. Two weeks after nerve injury, estimates of neuron number in DRG8 and DRG10 on both the operated and unoperated sides were more than twice the estimates obtained from control animals. Neuron number in the uninjured DRG9 was also elevated relative to that of control animals. Eight weeks after axotomy, differences in neuron number were less apparent. The mean cross-sectional area of DRG neurons was reduced 2 weeks after nerve injury in all DRGs. The decrease in mean area was the result of the addition of neurons to the smallest size classes. These data are discussed in the context of previous results showing that neurons are added as juvenile frogs grow to adult size (St. Wecker and Farel [1994] J. Comp. Neurol. 342:430-438). This addition results from the maturation of a population of incompletely differentiated neurons (Meeker and Farel [1997] J. Comp. Neurol. 389:569-576). The present results suggest that axotomy precipitates differentiation of these incompletely differentiated neurons, perhaps as a compensatory response to nerve injury.
Department of Physiology, School of Medicine, University of North Carolina, Chapel Hill 27599-7545, USA. farel@med.unc.edu
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Farel
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Boyer
D000367:Age Factors; D000818:Animals; D002452:Cell Count; D005727:Ganglia, Spinal / Q000166:cytology; D008161:Lumbosacral Region; D009416:Nerve Regeneration / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology* / Q000502:physiology*; D011892:Rana catesbeiana; D013127:Spinal Nerves / Q000166:cytology / Q000293:injuries*