Molecular cloning, sequence analysis, and characterization of a penicillin-resistant DD-carboxypeptidase of Myxococcus xanthus.
We have cloned a gene, pdcA, from the genomic library of Myxococcus xanthus with an oligonucleotide probe representing conserved regions of penicillin-resistant DD-carboxypeptidases. The amino- and carboxy-terminal halves of the predicted pdcA gene product showed significant sequence similarity to N-acetylmuramoyl-L-alanine amidase and penicillin-resistant DD-carboxypeptidase, respectively. The pdcA gene was expressed in Escherichia coli, and the characteristics of the gene product were similar to those of DD-carboxypeptidase (VanY) of vancomycin-resistant enterococci. No apparent changes in cell growth, sporulation, or germination were observed in pdcA deletion mutants.
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan. kimura@ag.kagawa-u.ac.jp
Y
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Kimura
Y
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Takashima
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Tokumasu
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Sato
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D002268:Carboxypeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli; D015698:Genomic Library; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry; D015345:Oligonucleotide Probes; D010403:Penicillin Resistance / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase
D001426:Bacterial Proteins; D008565:Membrane Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D002268:Carboxypeptidases; C120660:PdcA protein, Myxococcus xanthus; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; C117912:VanY protein, Bacteria; D009238:N-Acetylmuramoyl-L-alanine Amidase