Semen specific gamma-glutamyltransferase carries ABH antigens: a sandwich ELISA for simultaneous semen detection and its ABO blood typing.
Semen type of gamma-glutamyltransferase (gamma-GTP) is different from the membrane bound type of the enzyme in both biochemical and immunological properties, and consists of two subunits (150 and 95 kDa). We found that anti-ABH antibodies recognize a 150-kDa subunit of seminal gamma-GTP by Western blot and immunoprecipitation analyses. Using SG2, one of anti-semen specific gamma-GTP monoclonal antibodies which we had produced, and anti-ABH antibodies, we established a sandwich ELISA for identifying human seminal gamma-GTP and its ABO type simultaneously. This sandwich ELISA allows ABO typing of highly diluted semen. The dilutions for ABO typing were 10(5) times for A or O, and 10(4) times for B. Furthermore, ABO typing of semen was successfully performed by this ELISA, even in the mixed presence of vaginal fluid, saliva and blood. Thus, seminal gamma-GTP carries ABH antigens and the sandwich ELISA with SG2 and anti-ABH antibodies enables ABO typing of semen. The sandwich ELISA is extremely useful for ABO typing originated from semen in the mixture of biological fluids.
Department of Legal Medicine, Fukushima Medical University, School of Medicine, Japan. sumiko@cc.fmu.ac.jp
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Abe
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Gunji
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Kunii
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Kuraya
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Fujita
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Hiraiwa
D000017:ABO Blood-Group System / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000379:methods; D001788:Blood Grouping and Crossmatching; D001826:Body Fluids / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D015151:Immunoblotting; D008297:Male; D011233:Precipitin Tests; D012463:Saliva / Q000737:chemistry; D012661:Semen / Q000201:enzymology*; D012680:Sensitivity and Specificity; D005723:gamma-Glutamyltransferase / Q000276:immunology*
D000017:ABO Blood-Group System; D000941:Antigens; D005723:gamma-Glutamyltransferase