Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice.
The purpose of the present study was to investigate the in vitro and in vivo effects of aluminum sulfate on delta-aminolevulinic acid dehydratase (ALA-D) activity from the brain, liver and kidney of adult mice (Swiss albine). In vitro experiments showed that the aluminum sulfate concentration needed to inhibit the enzyme activity was 1.0-5.0 mM (N = 3) in brain, 4.0-5.0 mM (N = 3) in liver and 0.0-5.0 mM (N = 3) in kidney. The in vivo experiments were performed on three groups for one month: 1) control animals (N = 8); 2) animals treated with 1 g% (34 mM) sodium citrate (N = 8) and 3) animals treated with 1 g% (34 mM) sodium citrate plus 3.3 g% (49.5 mM) aluminum sulfate (N = 8). Exposure to aluminum sulfate in drinking water inhibited ALA-D activity in kidney (23.3 +/- 3.7%, mean +/- SEM, P < 0.05 compared to control), but enhanced it in liver (31.2 +/- 15.0%, mean +/- SEM, P < 0.05). The concentrations of aluminum in the brain, liver and kidney of adult mice were determined by graphite furnace atomic absorption spectrometry. The aluminum concentrations increased significantly in the liver (527 +/- 3.9%, mean +/- SEM, P < 0.05) and kidney (283 +/- 1.7%, mean +/- SEM, P < 0.05) but did not change in the brain of aluminum-exposed mice. One of the most important and striking observations was the increase in hepatic aluminum concentration in the mice treated only with 1 g% sodium citrate (34 mM) (217 +/- 1.5%, mean +/- SEM, P < 0.05 compared to control). These results show that aluminum interferes with delta-aminolevulinate dehydratase activity in vitro and in vivo. The accumulation of this element was in the order: liver > kidney > brain. Furthermore, aluminum had only inhibitory properties in vitro, while in vivo it inhibited or stimulated the enzyme depending on the organ studied.
Departamento de Química, Universidade Federal de Santa Maria, RS, Brasil.
M R
MR
Schetinger
C D
CD
Bonan
V M
VM
Morsch
D
D
Bohrer
L M
LM
Valentim
S R
SR
Rodrigues
D000534:Alum Compounds / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002951:Citrates; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000534:Alum Compounds; D002951:Citrates; C041524:aluminum sulfate; D000623:Porphobilinogen Synthase