Determination of 2,3-benzodiazepine derivatives in rat plasma by high-performance liquid chromatography.
A simple high-performance liquid chromatographic method with ultraviolet detection at 240 nm for determination of a novel AMPA/kainate antagonist 1-(4'-aminophenyl)-3,5-dihydro-7,8-dimethoxy-2,3-benzodiazepine (2,3-BZ 6), and its derivatives in rat plasma is described. The procedure involves a fast extraction of the drugs from the plasma spiked with an internal standard. The samples are applied to a pre-packed glass column and drugs are eluted using ethyl acetate. A linear response was observed over the examined concentration range. The lower limit of detection of 2,3-BZ 6 was 5.5 ng/ml. The assay has been used to determine the time course of plasma levels of the 2,3-benzodiazepine derivatives in Sprague-Dawley rats.
Rizzo
M
M
Chair of Chemistry, School of Pharmacy, University of Catanzaro, Italy.
De Sarro
G
G
Gitto
R
R
Zappalà
M
M
Chimirri
A
A
D000818:Animals; D001569:Benzodiazepines / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018691:Excitatory Amino Acid Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
C120593:1-(4'-aminophenyl)-3,5-dihydro-7,8--dimethoxy-2,3-benzodiazepine; D018691:Excitatory Amino Acid Antagonists; D001569:Benzodiazepines