Inhibition of various functions in murine peritoneal macrophages by aflatoxin B1 exposure in vivo.
Aflatoxin B1 (AFB1) has been known to impair specific and nonspecific immunity. In the present study, we tested various functions of murine peritoneal macrophages that were isolated and stimulated with LPS after AFB1 (400 microg/5 ml/kg) was administered every other day for 2 weeks. AFB1 decreased phagocytosis and the production of superoxide anion (O2-) and hydrogen peroxide (H2O2), compared to those of corn oil-treated control group. In addition, the production of NO and TNF-alpha was decreased in macrophages of AFB1-treated mice. In vitro antitumor activity of in vivo AFB1-treated macrophages was reduced against target cell, L929. Taken together, these results suggested that AFBI might have the immunosuppressive effect on macrophages after in vivo exposure, which was related to the antitumor activity reduction.
College of Pharmacy, Sungkyunkwan University, Suwon City, Korea.
E Y
EY
Moon
D K
DK
Rhee
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S
Pyo
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003864:Depression, Chemical; D006861:Hydrogen Peroxide / Q000378:metabolism; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D002273:Carcinogens; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D009569:Nitric Oxide; D016604:Aflatoxin B1; D006861:Hydrogen Peroxide