Chromosome 16 allelic loss analysis of a large set of microdissected prostate carcinomas.
OBJECTIVE
To perform loss of heterozygosity (LOH) analysis on chromosome 16 in 102 highly purified DNA samples isolated from one or more adenocarcinomas, prostatic intraepithelial neoplasia (PIN), and matched benign prostatic epithelium from 95 radical prostatectomy patients.


METHODS
Specimens were procured by microdissection of frozen tissue samples, thus ensuring that highly select pure populations of cells were obtained for DNA extraction and LOH analysis. Multiple microsatellite markers were used to determine allelic loss on chromosome 16q.


RESULTS
Overall loss on 16q was seen in 31% of the cancers, and occurred more frequently in high stage cancers than low stage cancers. In contrast, allelic loss in PIN failed to exceed 6% at any of the loci that were examined.


CONCLUSIONS
These results suggest that inactivation of a putative tumor suppressor gene on 16q may be involved in the progression of some prostate cancers.
Strup
S E
SE
Surgery Branch and Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland, USA.
Pozzatti
R O
RO
Florence
C D
CD
Emmert-Buck
M R
MR
Duray
P H
PH
Liotta
L A
LA
Bostwick
D G
DG
Linehan
W M
WM
Vocke
C D
CD
D000230:Adenocarcinoma / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D019048:Prostatic Intraepithelial Neoplasia / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm