Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom.
A simple, rapid, and sensitive diagnostic kit for detecting Thai cobra (Naja kaouthia) venom was developed using latex particles sensitized with venom specific immunoglobulin. The kit is capable of detecting 25-50 ng/ml of Thai cobra venom. The capability was not affected by human plasma. Specificity of the kit was proven using snake venoms from Vipera russelli, Calloselasma rhodostoma, Trimeresurus albolabris, Naja siamensis, Ophiophagus hannah, and Bungarus fasciatus. The diagnostic kit does not lose its capability under refrigeration for two months and by lyophilization.
Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.
O
O
Khow
N
N
Wongtongkam
N
N
Pakmanee
T
T
Omori-Satoh
V
V
Sitprija
D002846:Chromatography, Affinity; D003429:Cross Reactions; D015925:Cryopreservation; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D004546:Elapid Venoms / Q000032:analysis*; D005612:Freeze Drying; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007136:Immunoglobulins / Q000737:chemistry*; D007841:Latex Fixation Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D012910:Snake Venoms / Q000032:analysis*
D004546:Elapid Venoms; D007074:Immunoglobulin G; D007136:Immunoglobulins; D012910:Snake Venoms