Leukemia inhibitory factor expression is not induced in activated microglia and reactive astrocytes in response to rat basal forebrain cholinergic lesion.
In adult intact rat brain, leukemia inhibitory factor (LIF) mRNA has been found to be constitutively expressed in basal forebrain cholinergic neurons. To reveal a functional role of LIF in neurodegenerative events, the cellular expression pattern of LIF was determined by combining in situ hybridization and immunocytochemistry after specific and selective degeneration of basal forebrain cholinergic cells by a single intracerebroventricular application of the cholinergic immunotoxin 192IgG-saporin. Although basal forebrain cholinergic lesion resulted in a dramatic activation of micro- and astroglial cells at the lesion site, LIF mRNA expression was not detected in any of the lesion-induced activated glial cell types. As the cholinergic immunotoxin exerts its degenerative action by the ribosome-inactivating property of saporin, the lack of glial LIF induction might be due to the incapability of the dying cholinergic cell to form and release factors which induce LIF expression in activated glial cells.
Paul Flechsig Institute for Brain Research, University of Leipzig, Medical Faculty, Germany.
R
R
Lemke
S
S
Rossner
R
R
Schliebs
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D018678:Cholinergic Agents / Q000378:metabolism; D002799:Cholinergic Fibers / Q000187:drug effects / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006131:Growth Inhibitors / Q000096:biosynthesis*; D000922:Immunotoxins / Q000378:metabolism / Q000633:toxicity; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases; D016548:Prosencephalon / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D018678:Cholinergic Agents; D005904:Glial Fibrillary Acidic Protein; D006131:Growth Inhibitors; D000922:Immunotoxins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D012333:RNA, Messenger; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins