O-(fluoresceinylmethyl)hydroxylamine (OFMHA): a reagent for the preparation of fluorescent O-(fluoresceinylmethyl)oxime (FMO)-steroid conjugates.
The 5 and 6-isomers of O-(fluoresceinylmethyl)hydroxylamine reacted with a representative sample of oxo-steroids (6-oxoestradiol, estrone, norethindrone, cortisol, progesterone, and digitoxin-dialdehyde) to produce O-(fluoresceinylmethyl)oxime conjugates in a single step in 24-84% yield after preparative high performance liquid chromotography.
Adamczyk
M
M
Department of Chemistry, Abbott Laboratories, Abbott Park, Illinois, USA. maciej.adamczyk@abbott.com
Chen
Y Y
YY
Grote
J
J
Mattingly
P G
PG
D002851:Chromatography, High Pressure Liquid; D004074:Digitoxin / Q000737:chemistry; D004958:Estradiol / Q000031:analogs & derivatives / Q000737:chemistry; D004970:Estrone / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes; D006854:Hydrocortisone / Q000737:chemistry; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009640:Norethindrone / Q000737:chemistry; D010091:Oximes / Q000737:chemistry*; D011374:Progesterone / Q000737:chemistry; D013256:Steroids / Q000737:chemistry*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C118023:O-(fluoresceinylmethyl)hydroxylamine; D010091:Oximes; D013256:Steroids; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; C012396:6-ketoestradiol; D004074:Digitoxin; D009640:Norethindrone; D006854:Hydrocortisone