Synthesis of sugar fatty acid esters by modified lipase.
A simple synthesis of sugar fatty acid esters was developed in a nonaqueous solution using lipase modified by synthetic detergent. Esterification of sugar was accelerated by continuous removal of water from the reaction mixture with a molecular sieve. When glucose and palmitic acid (1:1 by mole) were used as the starting substrates, more than 90% of glucose was converted to its ester in this system. The resultant product was 6-O-palmitoylglucose. Other mono- or disaccharides were also esterified by the modified lipase with high yield. It was shown that the modified lipase might act as a catalyst for the synthesis of sugar fatty acid esters.
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8642, Japan. wakako@ss.nfri.affrc.go.jp
W
W
Tsuzuki
Y
Y
Kitamura
T
T
Suzuki
S
S
Kobayashi
D002241:Carbohydrates / Q000138:chemical synthesis*; D005227:Fatty Acids / Q000138:chemical synthesis*; D008049:Lipase / Q000737:chemistry*; D011549:Pseudomonas / Q000201:enzymology; D013997:Time Factors
D002241:Carbohydrates; D005227:Fatty Acids; D008049:Lipase