Disruption of six Saccharomyces cerevisiae novel genes and phenotypic analysis of the deletants.
As a part of the EUROFAN programme, six open reading frames from Saccharomyces cerevisiae (YNL083w, YNL086w, YNL087w, YNL097c, YDL100c and YOR086c) were disrupted in two genetic backgrounds, FY1679 and W303. Individual deletions in diploid strains and tetrad analysis of heterozygous deletants revealed that none of them is essential. Basic phenotypic analysis did not reveal any significant difference between the parental and mutant strains. Although YNL087w and YOR086c are 55% identical, the double disruptant also behaves the same as the parental cells. Ydl100p seems to be involved in metal detoxification, the phenotype of the null mutants being enhanced when the assays are performed at 37 degrees C.
Centro de Biología Molecular Severo Ochoa, CSIC and UAM, Cantoblanco, 28049-Madrid, Spain.
S
S
Zúñiga
J
J
Boskovic
A
A
Jiménez
J P
JP
Ballesta
M
M
Remacha
D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008670:Metals / Q000494:pharmacology; D009841:Oligonucleotides; D016366:Open Reading Frames; D010641:Phenotype; D020161:Physical Chromosome Mapping; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D005656:Fungal Proteins; D008670:Metals; D009841:Oligonucleotides