Effects of saiboku-to on the survival of human eosinophils.
In recent years, bronchial asthma has come to be regarded as a chronic inflammatory disease of the respiratory tract, with mast cells, lymphocytes and eosinophils playing important roles in its pathogenesis. Proteins contained in eosinophil granules, especially major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil-derived neurotoxin (EDN) and eosinophil peroxidase (EPO), can cause tissue injury. When stimulated, eosinophils release mediators such as leukotriene C4 (LTC4) and platelet activating factors (PAF). Thus, they are recognized as effector cells that are actively involved in the development of allergic inflammation. In this study, eosinophils from healthy volunteers were used to investigate the effects of Saiboku-to on eosinophils whose survival had been prolonged through stimulation with eosinophil-activating cytokines such as interleukin (IL)-3, IL-5 and granulocyte macrophage colony stimulating factors (GM-CSF). As a result, the cytokine-enhanced survival of eosinophils was significantly shortened by the addition of Saiboku-to. These findings suggest that Saiboku-to has the potential to inhibit allergic responses by directly affecting eosinophils which are related to allergic inflammation.
Tohda
Y
Y
Fourth Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Haraguchi
R
R
Kubo
H
H
Muraki
M
M
Fukuoka
M
M
Nakajima
S
S
D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004804:Eosinophils / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015848:Interleukin-5 / Q000037:antagonists & inhibitors / Q000494:pharmacology; D020835:Medicine, Kampo; D013997:Time Factors
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; D007377:Interleukin-3; D015848:Interleukin-5; C045086:saiboku-to; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor