Swab-based enzyme immunoassay system for detection of meat residues on food contact surfaces as a hygiene monitoring tool.
An enzyme immunoassay system based on the use of a macroporous swab as a solid phase for the capture and subsequent immunoenzymatic detection of immunoglobulin G (IgG) from meat residues on food contact surfaces was developed as a hygiene-monitoring tool. Moistened polyester swabs coated with anti-bovine or anti-chicken IgG were rubbed on the test surface, and the captured IgG was subsequently detected directly on the swabs by brief sequential reactions with anti-bovine or anti-chicken IgG-peroxidase conjugate and chromogenic peroxidase substrate.
Blais
B W
BW
Laboratory Services Division, Canadian Food Inspection Agency, Ottawa, Ontario.
D000818:Animals; D002417:Cattle; D004866:Equipment Contamination; D005511:Food Handling; D006920:Hygiene; D007124:Immunoenzyme Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D008460:Meat; D011200:Poultry
D007074:Immunoglobulin G