Estrogen regulation of intestinal calcium absorption in the intact and ovariectomized adult rat.
Studies were carried out to examine the mechanism of action of estrogen on intestinal calcium absorption in the rat. Three-month-old Wistar rats were sham-operated or ovariectomized (OVX). They were fed a diet containing 0.4% Ca, 0.4% P, and 2000 IU vitamin D3/kg. Eight weeks after operation, both OVX and sham-operated rats were randomly assigned to eight treatment groups. Five groups received per 100 g of body weight 12.5 ng calcitriol (1, 25-dihydroxyvitamin D3); 7.5 microg of estradiol-benzoate; 7.5 microg of estradiol-benzoate and 0.1 mg of ICI 182780; 12.5 ng of calcitriol and 0.1 mg of ICI 182780; and 0.1 mg of ICI 182780, respectively. Three groups received the various vehicles used. Intestinal calcium absorption was measured in vivo using single pass perfusion of the duodenum. OVX did not change intestinal calcium absorption. A pharmacological dose of estradiol-benzoate caused a significant increase in intestinal absorption of calcium, which was comparable to that of a pharmacological dose of calcitriol in both OVX and sham-operated rats. Estrogen-induced rise in intestinal calcium absorption was completely blocked to basal level by the pure estrogen receptor (ER) antagonist ICI 182780. In contrast, ICI 182780 did not antagonize calcitriol-enhanced intestinal calcium absorption. Our findings suggest that estrogen stimulates intestinal calcium absorption via an ER.
Department of Endocrinology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.
M
M
Ten Bolscher
J C
JC
Netelenbos
R
R
Barto
L M
LM
Van Buuren
W J
WJ
Van der vijgh
D000818:Animals; D002117:Calcitriol / Q000097:blood / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D005260:Female; D000077267:Fulvestrant; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism
D004965:Estrogen Antagonists; D004967:Estrogens; D011960:Receptors, Estrogen; C074283:estradiol 3-benzoate; D000077267:Fulvestrant; D004958:Estradiol; D002117:Calcitriol; D002118:Calcium