Concentration- and time-dependent effects of gamma-linolenic acid supplementation to tumor cells in culture.
Gamma-linolenic acid (GLA) supplemented to neuroblastoma SK-N-BE, tubal carcinoma TG and colon carcinoma SW-620 cells was incorporated into phospholipids in all the cell lines (although to different extents), in a concentration- and time-dependent manner. All the cell lines were able to metabolize GLA to arachidonic acid, SK-N-BE being the most active. Supplementation with low GLA concentrations for short periods was not sufficient to impair cell proliferation; only higher amounts of GLA had an anti-proliferative effect also in short times. In these conditions, the antiproliferative effect of GLA is probably due to cellular dysfunction caused by fatty acid modifications.
Department of Biochemistry G. Moruzzi, University of Bologna, Italy.
S
S
Hrelia
A
A
Pession
R
R
Buda
A
A
Lorenzini
D F
DF
Horrobin
P L
PL
Biagi
A
A
Bordoni
D016718:Arachidonic Acid / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured; D017965:gamma-Linolenic Acid / Q000494:pharmacology*
D005227:Fatty Acids; D010743:Phospholipids; D016718:Arachidonic Acid; D017965:gamma-Linolenic Acid; D013936:Thymidine