Coenzyme Q10 depletion and mitochondrial energy disturbances in rejection development in patients after heart transplantation.
Sixty endomyocardial biopsies (EMB) and whole blood or plasma samples from 34 patients after heart transplantation (HTx-pts) were studied. Acute rejection of the transplanted heart was histologically graded as: 0 (without), 0-1 (incipient), 1 (mild), 2 (moderate). The level of coenzyme Q10 (CoQ10) in 28 EMB was estimated by HPLC. Mitochondrial respiratory chain function and energy production were measured in 60 EMB. This study is the first report showing a correlation between: (a) histological signs of rejection in the human transplanted heart and (b) CoQ10 level of EMB, CoQ10 blood level, and mitochondrial bioenergetic processes: inhibition in FAD-part, but not in NAD-part of respiratory chain. In all patients after heart transplantation (HTx-pts) the dynamic balance between total antioxidant status and degree of oxidative stress was disturbed.


CONCLUSIONS
CoQ10 level and mitochondrial bioenergetic functions of EMB contribute to the explanation of pathobiochemical mechanisms of origin and development rejection of human transplanted heart. We suppose that estimation of EMB CoQ10 level could be used as a bioenergetic marker of rejection development in human transplanted heart. CoQ10 therapy could contribute to the prevention of rejection of the transplanted heart.
Gvozdjáková
A
A
Pharmacobiochemical Laboratory of Medical Faculty, Comenius University, Bratislava, Slovak Republic.
Kucharská
J
J
Mizera
S
S
Braunová
Z
Z
Schreinerová
Z
Z
Schrameková
E
E
Pechán
I
I
Fabián
J
J
D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D003067:Coenzymes; D004734:Energy Metabolism; D006084:Graft Rejection / Q000378:metabolism*; D016027:Heart Transplantation / Q000473:pathology / Q000502:physiology*; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000378:metabolism*; D010101:Oxygen Consumption; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D003067:Coenzymes; D014451:Ubiquinone; D014810:Vitamin E; D008315:Malondialdehyde; C024989:coenzyme Q10