Modeling the aerobic growth and decline of Staphylococcus aureus as affected by pH and potassium sorbate concentration.
The effects of pH (5.0, 5.2, 5.4, 5.6, and 5,8) and concentration of potassium sorbate (10.0 and 16.6 mM) at two water activity values (0.90 and 0.92) on the aerobic growth and decline of Staphylococcus aureus ATCC 6538P, 196-E, and FDA-C243 were studied using brain-heart infusion broth. The inoculum was approximately 4 to 5 log CFU/ml, and the incubation temperature was 30 degrees C. Samples were periodically enumerated on tryptic soy agar. The Gompertz model was used to obtain microbial growth parameters, specific growth rate was obtained as a derived parameter, and the inhibition index was calculated. A linear model was fitted in cases of bacteriostatic or bactericidal action of the treatment. The ATCC 6538P strain showed the highest resistance in the range of tested conditions. Microbial behavior was modeled considering the main controlling factors, and a response surface methodology was used to determine the effects of undissociated acid concentration and pH. These results can be used to establish treatment conditions for microorganism growth or inhibition.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, Argentina.
L
L
Giannuzzi
E
E
Contreras
N
N
Zaritzky
D000332:Aerobiosis; D015169:Colony Count, Microbial; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D013011:Sorbic Acid / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
D003470:Culture Media; D013011:Sorbic Acid