Immunoblotting technique for the detection of allergens of Aspergillus fumigatus: influence of Nonidet P-40 on the sensitivity.
Immunoblotting provides a useful technique for the study of antigens, antibodies and allergens. To overcome problems regarding the loss of antigenic properties during the blotting and developing procedures, several solutions have been described. The inclusion of Nonidet P-40, recommended to increase the sensitivity of developing procedures for immunoblots, in an existing procedure for the detection of allergens of Aspergillus fumigatus, however, led to decreased sensitivity of the method.
National Institute of Public Health and the Environment, Microbiological Laboratory for Health Protection, Bilthoven, The Netherlands.
L M
LM
Wijnands
W D
WD
Deisz
F M
FM
van Leusden
D000485:Allergens / Q000032:analysis; D000946:Antigens, Fungal / Q000032:analysis; D001232:Aspergillus fumigatus / Q000276:immunology*; D015151:Immunoblotting / Q000379:methods*; D007073:Immunoglobulin E / Q000097:blood; D017830:Octoxynol; D011092:Polyethylene Glycols
D000485:Allergens; D000946:Antigens, Fungal; D007073:Immunoglobulin E; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40