In vitro anticomplementary activity of hederagenin saponins isolated from roots of Dipsacus asper.
Anticomplementary activity of hederagenin and related saponins isolated from Dipsacus asper was investigated in vitro. HN saponin F (3) was most potent with IC50 value of 3.7x10(-5) M followed by 3-O-beta-D-glucopyranosyl-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-beta-L-+ ++arabi nopyranosyl hederagenin 28-O-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyrano side (8), 3-O-beta-L-arabinopyranosyl hederagenin 28-O-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranoside (5), dipsacus saponin A (4), and hederagenin (1) on the classical pathway (CP) of complement system, while the saponins 3-5 did not show the inhibition of hemolysis and rather increase the hemolysis on the alternative pathway (AP). However, all of C-3 monodesmosides [prosapogenin CP (2), dipsacus saponin B (6), and dipsacus saponin C (7)] evoked hemolysis directly on the erythrocytes.
Korea Research Institute of Bioscience & Biotechnology, KIST, Yusong, Taejon.
S R
SR
Oh
K Y
KY
Jung
K H
KH
Son
S H
SH
Park
I S
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Lee
K S
KS
Ahn
H K
HK
Lee
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology; D012503:Saponins / Q000276:immunology*; D012756:Sheep; D013256:Steroids
D000893:Anti-Inflammatory Agents; D003169:Complement Inactivator Proteins; D010936:Plant Extracts; D012503:Saponins; D013256:Steroids; D009828:Oleanolic Acid; C025763:hederagenin