RNA maturation of the rice SPK gene may involve trans-splicing.
A gene encoding a calcium-dependent seed-specific protein kinase (SPK) is abundantly expressed in developing rice seeds (Kawasaki, T et al. Gene (1993) 129, 183-189). Rice genomic clones encoding SPK were isolated using the entire cDNA fragment as a probe. Physical mapping of these genomic clones indicated that the genomic region corresponding to the entire cDNA was divided into two different regions, SPK-A and SPK-B, located on different rice chromosomes. The results of RACE-PCR analyses showed that the respective transcripts from SPK-A and SPK-B contained additional sequences which were not found in the SPK cDNA, and that these sequences were removed like introns during maturation of the SPK mRNA. These results suggest that two different RNAs were independently transcribed from SPK-A and SPK-B and joined, possibly by trans-splicing.
Mitsui Plant Biotehcnology Research Institute, TCI D-21, Sengen, Tsukuba, Japan. kawasaki@bs.aist-nara.ac.jp
T
T
Kawasaki
S
S
Okumura
N
N
Kishimoto
H
H
Shimada
K
K
Higo
N
N
Ichikawa
D001483:Base Sequence; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011494:Protein Kinases / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology; D020040:Trans-Splicing; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D018076:DNA, Complementary; D011494:Protein Kinases; D018749:RNA, Plant; D017871:Calcium-Calmodulin-Dependent Protein Kinases