Enhanced angiogenesis and growth of 12-lipoxygenase gene-transfected MCF-7 human breast cancer cells in athymic nude mice.
Transfection of the estrogen-dependent and poorly invasive MCF-7 human breast cancer cell line so that it stably overexpressed 12-lipoxygenase and secreted high levels of 12-hydroxyeicosatetraenoic acid when cultured with arachidonate resulted in rapid growth in athymic nude mice when compared with the parental line. This enhanced acquisition of tumor mass was a result of both increased cell proliferation and reduced apoptotic cell death and was accompanied by high angiogenic activity.
Connolly
J M
JM
Division of Nutrition and Endocrinology, American Health Foundation, Valhalla, NY 10595, USA.
Rose
D P
DP
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics*; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D002455:Cell Division; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000032:analysis; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000502:physiology
D019394:Ki-67 Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D001092:Arachidonate 12-Lipoxygenase