Participation of arterial baroreceptors input and peripheral vasopressin in the suppression of renal sympathetic nerve activity induced by central salt loading in conscious rats.
We examined whether renal sympathetic nerve activity (RSNA) is suppressed in response to intracerebroventricular (i.c.v.) administration of hypertonic saline (HS) in conscious rats. RSNA was suppressed by i.c.v. administration of HS (0.3 M, 0.67 M, and 1.0 M, 1 microl/min for 20 min) in a concentration-dependent manner, which was attenuated under pentobarbital anesthesia. To elucidate mechanisms responsible for central HS-induced decrease in RSNA, possible involvement of arterial baroreceptors and peripheral arginine vasopressin (AVP) secreted from the posterior pituitary gland was examined using sinoaortic denervated (SAD) rats and non-peptide vasopressin receptor antagonists. The maximum suppression of RSNA (-81.5 +/- 5.5%) in control rats was significantly attenuated to -32.5 +/- 6.7% in SAD rats and to -55.8 +/- 5.7% in rats pretreated with intravenous vasopressin V1 receptor antagonist, OPC-21268 (5 mg/kg, i.v.). However, in SAD rats, pretreatment with vasopressin V1 receptor antagonist did not further affect the RSNA inhibition induced by central salt loading. The results suggest that the suppression of RSNA during central salt loading is mainly dependent on the arterial baroreceptors input and the 'additive' role of peripheral vasopressin.
Department of Physiology, Miyazaki Medical College, Japan.
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K
Kato
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Shirasaka
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T
Kunitake
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T
Hanamori
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H
Kannan
D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001158:Arteries / Q000187:drug effects / Q000294:innervation*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D004594:Electrophysiology; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D007276:Injections, Intraventricular; D007668:Kidney / Q000294:innervation* / Q000502:physiology; D008297:Male; D011311:Pressoreceptors / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012462:Saline Solution, Hypertonic; D012849:Sinoatrial Node / Q000502:physiology; D013562:Sympathectomy; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D014667:Vasopressins / Q000037:antagonists & inhibitors / Q000502:physiology*
D065092:Antidiuretic Hormone Receptor Antagonists; D012462:Saline Solution, Hypertonic; D014667:Vasopressins