Influence of a protein hydrolysate from green algae on the activity of some ATPase systems in frog skeletal muscle.
The present study investigated the effect of a protein hydrolysate from green algae cultured in the Bulgarian region of Rupy, on the enzyme activity of frog skeletal muscle. The activity of pure Mg(2+)-ATPase, Mg2+,Ca(2+)-ATPase, NaHCO3-stimulated Mg(2+)-ATPase and the latter in the presence of the inhibitors NaSCN and NaN3 in mitochondrial (B-3) and membrane (B-12) fractions were determined before and after treatment with the protein hydrolysate from green algae (30 and 300 micrograms/ml). The differences between ATPase activity of mitochondrial and membrane fractions were described and it was established that in the B-3 fraction, the activity of the NaHCO3-stimulated Mg(2+)-ATPase and Ca(2+)-dependent Mg(2+)-ATPase were accelerated by increasing concentrations of the algae protein hydrolysate. Irrespective of the different (equal or inverse) dose-dependent effects, the protein hydrolysate stimulated Mg(2+)-ATPase and that inhibited by NaSCN an NaN3 bicarbonate-stimulated Mg(2+)-ATPase activity. In most of the probes, the protein hydrolysate produced some increase in enzyme activity of NaHCO3-stimulated Mg(2+)-ATPase and Ca(2+)-dependent Mg(2+)-ATPase in B-12 fractions. The observed properties of the algae protein hydrolysate suggest that it is capable of stimulating enzyme processes in addition to having some antitoxic effect in skeletal muscle.
Ivanov
R
R
Biological Faculty, Sofia University, Bulgaria.
Georgieva
B
B
Naumova
P
P
Mileva
K
K
Radicheva
N
N
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D000460:Chlorophyta / Q000737:chemistry*; D066298:In Vitro Techniques; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000201:enzymology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D011492:Protein Hydrolysates / Q000494:pharmacology*; D011895:Rana ridibunda; D013268:Stimulation, Chemical
D001639:Bicarbonates; D011492:Protein Hydrolysates; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium