Mucosal and systemic candidiasis in IL-8Rh-/- BALB/c mice.
Germ-free BALB/c mice, genetically engineered to be deficient for interleukin-8 (IL-8) receptor homolog (IL-8Rh-/-), were more susceptible to gastric candidiasis after oral challenge and to acute systemic candidiasis after intravenous challenge than IL-8Rh+/+ controls. In comparison to IL-8Rh+/+ mice, the IL-8Rh-/- mice had slower influx of polymorphonuclear neutrophils (PMN) into Candida albicans-infected tissues and a lower percentage of PMN in peritoneal exudate cells (PEC) elicited with heat-killed C. albicans. PEC from IL-8Rh-/- mice exhibited less luminol-dependent chemiluminescence in response to C. albicans and did not kill C. albicans hyphae as well as PEC from IL-8Rh+/+ mice. C. albicans-colonized IL-8Rh-/- mice showed no histological evidence of systemic candidiasis. These results suggest a role for the IL-8Rh in murine resistance to gastric and acute systemic candidiasis, but not in resistance to systemic candidiasis of endogenous origin.
Department of Surgery, University of Wisconsin Medical School, Madison 53706-1532, USA. balish@surgery.wisc.edu
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Balish
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Wagner
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Vazquez-Torres
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D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D005260:Female; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D007410:Intestinal Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009504:Neutrophils / Q000276:immunology; D018123:Receptors, Interleukin / Q000235:genetics / Q000502:physiology*; D023062:Receptors, Interleukin-8A
D015703:Antigens, CD; D007202:Indicators and Reagents; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D008165:Luminol