Human release factor eRF1: structural organisation of the unique functional gene on chromosome 5 and of the three processed pseudogenes.
In lower and higher eukaryotes, a family of tightly related proteins designated eRF1 (for eukaryotic release factor 1) catalyses termination of protein synthesis at all three stop codons. The human genome contains four eRF1 homologous sequences localised on chromosomes 5, 6, 7 and X. We report here the cloning and the structural analysis of the human eRF1 gene family. It appears that the gene located on chromosome 5 alone is potentially functional, whereas the other three sequences resemble processed pseudogenes. This is the first description of the structural organisation of the human eRF1 gene, which has been remarkably conserved during evolution and which is essential in the translation termination process.
Département de Biochimie et Biologie Moléculaire et UPR41 CNRS, Faculté de Médecine, Rennes, France.
L
L
Guenet
B
B
Toutain
I
I
Guilleret
B
B
Chauvel
L L
LL
Deaven
J L
JL
Longmire
J Y
JY
Le Gall
V
V
David
A
A
Le Treut
D001483:Base Sequence; D002895:Chromosomes, Human, Pair 5; D002896:Chromosomes, Human, Pair 6; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D003360:Cosmids; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors / Q000737:chemistry* / Q000235:genetics*; D011544:Pseudogenes; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014960:X Chromosome
C489002:ETF1 protein, human; D010454:Peptide Termination Factors