Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules.
The CDw108 glycoprotein is expressed on the surface of some leukemic cell lines, erythrocytes and on activated lymphocytes. Its surface expression is rapidly upregulated following various activating stimuli (PHA, PWM, Con A, PMA, anti-CD3) and subsequently gradually decreases. The molecule is anchored in the membrane via glycosylphosphatidylinositol (GPI) moiety, it has molecular mass of 75-80 kDa and pI of 5.0-5.5. Endoglycosidase F and H reduce its apparent size as determined by SDS PAGE by approx. 15 and 22 kDa, respectively. It is a component of large, detergent-resistant GPI-complexes associated with protein kinases. In addition to the previously described identity of CDw108 with the JMH blood group antigen, we demonstrate here its identity to the previously described glycoprotein recognized by monoclonal antibodies H105 and KS.2, and exclude its identity with another GPI-anchored glycoprotein of similar size, melanotransferrin (gp97).
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
P
P
Angelisová
K
K
Drbal
J
J
Cerný
I
I
Hilgert
V
V
Horejsí
D015703:Antigens, CD / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D000951:Antigens, Neoplasm; D000954:Antigens, Surface / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D058950:Melanoma-Specific Antigens; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D039961:Semaphorins; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D058950:Melanoma-Specific Antigens; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C118753:SEMA7A protein, human; D039961:Semaphorins