Effects of berberine on arylamine N-acetyltransferase activity in human bladder tumour cells.
Berberine was used to determine inhibition of arylamine N-acetyltransferase (NAT) activity in human bladder tumour cells. The NAT activity was measured by HPLC assaying for the amounts of N-acetyl-2-aminofluorene (AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and remaining 2-aminofluorene (AF) and p-aminobenzoic acid (PABA). Two assay systems were performed, one with cellular cytosols, the other with intact bladder tumour cell suspensions. The NAT activity in human bladder tumour cells was inhibited by berberine in a dose-dependent manner, that is, the higher the concentration of berberine, the higher the inhibition of NAT activity. The values of apparent Km and Vmax calculated from cytosol NAT and intact cells were also decreased by berberine. This report is the first demonstration to show berberine did affect human bladder tumour cell NAT activity.
Chung
J G
JG
Department of Microbiology, China Medical College, Taichung, Taiwan, Republic of China.
Wu
L T
LT
Chu
C B
CB
Jan
J Y
JY
Ho
C C
CC
Tsou
M F
MF
Lu
H F
HF
Chen
G W
GW
Lin
J G
JG
Wang
T F
TF
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D001191:Arylamine N-Acetyltransferase / Q000037:antagonists & inhibitors*; D001599:Berberine / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005449:Fluorenes / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology
D002273:Carcinogens; D004791:Enzyme Inhibitors; D005449:Fluorenes; D001599:Berberine; C012177:2-aminofluorene; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid