Nickel release from orthodontic arch wires and cellular immune response to various nickel concentrations.
OBJECTIVE
Results from two previous clinical studies suggested that exposure to high nickel-containing orthodontic arch wires may induce hypersensitivity in certain individuals. The purpose of this study was to measure the amount of nickel released from three types of nickel-containing arch wires into a synthetic saliva in vitro, and determine if the concentrations were sufficient to elicit either cytotoxic (trypan blue exclusion test) or stimulatory (MTT test) responses in human peripheral blood mononuclear cells (PBMCs) derived from nickel-sensitive and nickel-nonsensitive individuals. PBMCs were exposed to five concentrations of nickel sulfate solutions ranging from 0-29 ppm, and results were compared, particularly at concentrations obtained from nickel release experiments.


RESULTS
The amount of nickel released into synthetic saliva ranged from 0.4-4.1 ppb. Wires subjected to a combination of soaking and cyclic straining released significantly more nickel than those that were soaked only (p </= 0.05), and NiTi wires released significantly more nickel than did stainless steel or nitrogen-implanted NiTi wires (p </= 0.05). For PBMCs, significant increased cell proliferation was not observed for any nickel concentration. PBMC cell death rates were highest at nickel concentrations of 29 ppm when the cells were cultured without a cell growth promoter (p </= 0.05), and MTT test values were significantly reduced at both 2.9 and 29 ppm when a growth promoter was included (p </= 0.05).


CONCLUSIONS
The maximum amount of nickel released from all tested arch wires was 700 times lower than the concentrations necessary to elicit cytotoxic reactions in human PBMCs.
Section of Biomaterials, Department of Adult Restorative Dentistry, University of Nebraska Medical Center College of Dentistry, Lincoln, Nebraska 68583-0750, USA.
W
W
Jia
M W
MW
Beatty
R A
RA
Reinhardt
T M
TM
Petro
D M
DM
Cohen
C R
CR
Maze
E A
EA
Strom
M
M
Hoffman
D000328:Adult; D001672:Biocompatible Materials; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D007111:Immunity, Cellular / Q000187:drug effects*; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008422:Materials Testing; D009532:Nickel / Q000009:adverse effects* / Q000276:immunology* / Q000493:pharmacokinetics; D009969:Orthodontic Wires / Q000009:adverse effects*; D012464:Saliva, Artificial; D013314:Stress, Mechanical; D014025:Titanium
D001672:Biocompatible Materials; D012464:Saliva, Artificial; D009532:Nickel; D014025:Titanium