Automated detection of Salmonella spp. in foods.
An automated method to detect salmonellae in foods was developed and tested in food samples intentionally contaminated with the test organisms. Liquid eggs, shell eggs, dry eggs, skim milk and chicken were spiked with Salmonella enteritidis, S. typhimurium or S. newport to yield 2 to 25 CFU per 25 g or ml of sample. Following pre-enrichment in universal pre-enrichment broth at 42 degrees C for 6 h (eggs and milk) or 16 h (chicken), Salmonella cells were captured by immunomagnetic beads coated with Salmonella antibody (Vicam, Watertown, MA). The beads were transferred to selective liquid media containing carbohydrate (dulcitol or xylose), amino acid (lysine or ornithine), and H2S indicator, and incubated at 42 degrees C in the BioSys instrument (MicroSys, Ann Arbor, MI). Salmonella positive samples were identified by black discoloration of the media during incubation, while negative samples remained colorless. These color changes were recorded by the instrument. All the artificially contaminated samples tested positive within 15-18 h, while control samples remained negative during 24 h incubation. The results agreed with standard identification procedures. A total of 24 h was required to detect 2 to 25 CFU of the pathogen in 25 g or ml of eggs and milk, and up to 36 h in chicken, compared to 72 h in the standard methods.
Department of Nutrition and Food Science, Wayne State University, Detroit, MI 48202, USA.
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D000818:Animals; D002645:Chickens / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D004531:Eggs / Q000382:microbiology; D005511:Food Handling / Q000295:instrumentation*; D008892:Milk / Q000382:microbiology; D011933:Reagent Kits, Diagnostic / Q000382:microbiology*; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors
D003470:Culture Media; D011933:Reagent Kits, Diagnostic