Microbial remediation of NTO in aqueous industrial wastes.
The bioremediation of aqueous wastes containing 5-nitro-1,2,4-triazol-3-one (NTO) was investigated. The microorganism used is a Bacillus licheniformis strain, isolated from the contaminated solutions by enrichment techniques. The biodegradation was carried out in the waste (15 g l-1 NTO) and proceeded through the nitroreduction of NTO, followed by the ring cleavage of the formed primary amine 5-amino-1,2,4-triazol-3-one (ATO). Both steps were optimized and according to the optimal conditions, the nitroreduction of NTO is total in 24 h, while the degradation of ATO requires 2 weeks of incubation. The end products of the biodegradation were carbon dioxide (40%), urea and a polar compound, assumed to be hydroxyurea. A mechanism of ATO ring cleavage was postulated in the light of experimental data, and led us to propose an overall degradation sequence for NTO.
Le Campion
L
L
Institut de Chimie des Substances Naturelles, C.N.R.S., Gif sur Yvette, France.
Vandais
A
A
Ouazzani
J
J
D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide; D007218:Industrial Microbiology; D009574:Nitro Compounds / Q000378:metabolism*; D013997:Time Factors; D014230:Triazoles / Q000378:metabolism*; D014508:Urea; D014871:Water Microbiology
D009574:Nitro Compounds; D014230:Triazoles; D002245:Carbon Dioxide; D014508:Urea; C108812:5-nitro-1,2,4-triazol-3-one