Identification and purification of IgE-reactive proteins in German cockroach extract.
Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.
Yong
T S
TS
Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea. tsyong212@yumc.yonsei.ac.kr
Lee
J S
JS
Lee
J
J
Park
S J
SJ
Jeon
S H
SH
Ree
H I
HI
Park
J W
JW
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D003058:Cockroaches / Q000737:chemistry*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D014020:Tissue Extracts / Q000737:chemistry*
D019476:Insect Proteins; D014020:Tissue Extracts; D007073:Immunoglobulin E