A simple and accurate method for generating co-dominant markers: an application of conformation-sensitive gel electrophoresis to linkage analysis in the silkworm.
A simple and sensitive method for linkage analysis is described, which is based on conformation-sensitive gel electrophoresis (CSGE). Using urea-containing agarose gels or a commercially available polyacrylamide-derived matrix, 13 polymorphic markers were newly identified for known genes of the silkworm, Bombyx mori, which had been scored as monomorphic by PCR-RFLP analysis. This method for detecting polymorphisms is quite sensitive, and can be performed with inexpensive reagents and apparatus that is available in most molecular biology laboratories.
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan. yaskoch@nises.affrc.go.jp
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Yasukochi
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D019992:Databases as Topic; D004586:Electrophoresis / Q000379:methods; D005260:Female; D005799:Genes, Dominant; D008040:Genetic Linkage; D005819:Genetic Markers; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers; D019476:Insect Proteins; D004247:DNA