Experimental induction of AGEs in fetal L132 lung cells changes the level of intracellular cathepsin D.
The effect of the carbonyl compound glyoxal on the induction of advanced glycation end products (AGEs) in the fetal epithelial lung cells L132 was investigated using immunohistochemical, immunoelectron microscopic, and biochemical methods. It was found that glyoxal treatment resulted in morphological changes of the cells and in the membranous and cytosolic localization of AGEs such as methyl-glyoxal-derived compounds, N-(carboxymethyllysine) (CML) and imidazolone. The formation of AGEs was accompanied with a change in the intracellular expression of cathepsin D and a loss of enzymatic activity.
Kasper
M
M
Faculty of Medicine, Technical University of Dresden, Dresden, Germany. mkas@rcs.urz.tu-dresden
Schinzel
R
R
Niwa
T
T
Münch
G
G
Witt
M
M
Fehrenbach
H
H
Wilsch-Bräuninger
M
M
Pehlke
K
K
Hofer
A
A
Funk
R H
RH
D000906:Antibodies; D002402:Cathepsin D / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D048429:Cell Size / Q000187:drug effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006037:Glyoxal / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000378:metabolism; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism; D008854:Microscopy, Electron; D011658:Pulmonary Fibrosis / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000906:Antibodies; D017127:Glycation End Products, Advanced; D007093:Imidazoles; D012333:RNA, Messenger; C117197:imidazolone; D006037:Glyoxal; C048496:N(6)-carboxymethyllysine; D002402:Cathepsin D; D008239:Lysine