Suppression of experimental corneal angiogenesis by focal X-ray irradiation.
OBJECTIVE
To investigate the effect of focal X-ray irradiation on experimental corneal angiogenesis in the rabbit.


METHODS
A gelatin hydrogel sheet impregnated with basic fibroblast growth factor was implanted into the corneal stroma of rabbits; this induced corneal angiogenesis. After the first sign of corneal angiogenesis was noted, the corneal region was irradiated with a dose of 10 Gy or 20 Gy. The control rabbits received no irradiation. The eyes were examined by slitlamp biomicroscopy and photographed, over a period of 28 days. The maximum length and total surface area of corneal angiogenesis were quantified by computerized image analysis.


RESULTS
Corneal angiogenesis was noted on day 3 following implantation of the hydrogel sheet. In the rabbits irradiated with 10 Gy, the maximum length and total surface area of corneal angiogenesis were both significantly lower on day 4 and 7 following irradiation, compared to the respective measurement in the controls. In the rabbits irradiated with 20 Gy, the maximum length and total surface area of corneal angiogenesis were significantly lower between days 4 and 21, and between days 4 and 14, respectively, compared to the respective measurement in the controls.


CONCLUSIONS
Focal X-ray irradiation to the corneal region suppressed corneal angiogenesis in a dose-dependent manner. Focal X-ray irradiation may be beneficial in treating ocular angiogenesis.
Miyamoto
H
H
Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
Kimura
H
H
Yasukawa
T
T
Yang
C F
CF
Honda
Y
Y
Tabata
Y
Y
Ikada
Y
Y
Sasai
K
K
Ogura
Y
Y
D000818:Animals; D003315:Cornea / Q000098:blood supply* / Q000473:pathology / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008297:Male; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000532:radiotherapy*; D011817:Rabbits