Regulation of phosphorylation level and distribution of PTP36, a putative protein tyrosine phosphatase, by cell-substrate adhesion.
Recently we have cloned a putative protein tyrosine phosphatase, PTP36/PTPD2/pez, which possesses a domain homologous to the N-terminal half of band 4.1 protein. In mouse fibroblasts adhered to substrates, PTP36 was phosphorylated on serine residues. PTP36 was found to make complexes with serine/threonine kinase(s), which phosphorylated PTP36 in vitro. PTP36 was dephosphorylated rapidly when the cell-substrate adhesion was disrupted and it was phosphorylated again along with the reattachment of the cells to fibronectin. Rephosphorylation of PTP36 seemed to depend on actin polymerization since it was inhibited by cytochalasin D. The cell detachment also induced the translocation of PTP36 into the membrane-associated cytoskeletal fraction. Staurosporine and ML-9, which inhibited the phosphorylation of PTP36 in vivo, induced the translocation of PTP36 too. On the contrary, when the dephosphorylation of PTP36 was inhibited by okadaic acid, no translocation of PTP36 was induced by the cell detachment. These results demonstrate that the cell-substrate adhesion and cell spreading regulates the intracellular localization of PTP36 most likely through its phosphorylation and therefore, PTP36 may play important roles in the signal transduction pathway of cell-adhesion.
Ogata
M
M
Department of Oncology, Biomedical Research Center, Osaka University Medical School, Suita, Osaka 565-0871, Japan. mogata@ongene.med.osaka-u.ac.jp
Takada
T
T
Mori
Y
Y
Uchida
Y
Y
Miki
T
T
Okuyama
A
A
Kosugi
A
A
Sawada
M
M
Oh-hora
M
M
Hamaoka
T
T
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D051379:Mice; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D054558:Protein Tyrosine Phosphatases, Non-Receptor; D051381:Rats; D011917:Rats, Inbred Lew; D012694:Serine / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D013379:Substrate Specificity
D017931:DNA Primers; D004791:Enzyme Inhibitors; D012694:Serine; C093261:PTPN14 protein, human; D017027:Protein Tyrosine Phosphatases; D054558:Protein Tyrosine Phosphatases, Non-Receptor; D019311:Staurosporine