Evaluation of the use of two human cell lines for okadaic acid and DTX-1 determination by cytotoxicity assays and damage characterization.
Two human cell lines have been used, HEp-2 and (de)differentiated Caco-2, derived from a larynx and a colon carcinoma, respectively, with the aim of evaluating and characterizing the cytotoxicity of okadaic acid (OA) and related toxins. Effects of OA and dinophysistoxin-1 (DTX-1) on cell viability (neutral red uptake) and on cell morphology/cytoskeleton structure have been observed in both cell lines, though at different time exposures and with different concentrations. The morphological alteration was detected earlier than the viability inhibition in HEp-2 cells with both toxins and in Caco-2 cells with DTX-1. HEp-2 cells have shown to be more sensitive than the intestinal cell line and thus possibly suitable for screening of contaminated samples, while Caco-2 cells could be used for further investigating the possible mechanisms involved in diarrhoeic shellfish poisoning (DSP) toxins.
Laboratorio di Tossicologia Comparata ed Ecotossicologia, Istituto Superiore di Sanità, Roma, Italia.
G
G
Oteri
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Stammati
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Zampaglioni
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F
Zucco
D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003599:Cytoskeleton / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D020128:Inhibitory Concentration 50; D007822:Laryngeal Neoplasms; D008387:Marine Toxins / Q000633:toxicity*; D009499:Neutral Red; D019319:Okadaic Acid / Q000633:toxicity* / Q000031:analogs & derivatives; D011714:Pyrans / Q000633:toxicity*; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004396:Coloring Agents; D008387:Marine Toxins; D011714:Pyrans; D014404:Tubulin; D019319:Okadaic Acid; D009499:Neutral Red; C051904:dinophysistoxin 1