13q14 deletion in non-Hodgkin's lymphoma: correlation with clinicopathologic features.
OBJECTIVE
13q14 deletion frequently occurs as a single anomaly in chronic lymphocytic leukemia (CLL) with favorable prognosis. This study was performed to assess the distribution of 13q14 deletion in non-Hodgkin's lymphoma (NHL) and to analyze its correlation with salient clinicopathologic features.


METHODS
One hundred and twenty-five NHL were analyzed by cytogenetics and by interphase fluorescence in situ hybridization (FISH), using a 13q14 cosmid probe recognizing DNA sequences between the Rb gene and the D13S25 marker. Clinical records all patients were surveyed.


RESULTS
A 13q14 rearrangement was present in the stemline in 10 patients; 15 additional cases were shown by FISH to carry 13q14 deletion in 55-90% of the interphase cells, giving a 20% overall incidence for this anomaly. Six of 44 patients had a low-grade NHL, 14/28 had mantle cell lymphoma (MCL), 5/42 had a high grade NHL (p<0.0001). There was not correlation between 13q, karyotype status and complexity. A statistically significant association was found between 13q-, presence of splenomegaly and PB involvement, lower probability of attaining complete remission (CR) and shorter survival. These findings were not simply a function of the association of 13q- with MCL. In multivariate analysis, a complex karyotype had prognostic importance (p=0.0078), along with age (p=0.01), histology (p=0.001), LDH (p=0.03), PS (p=0.001), sex (p=0.03) and splenomegaly (p=0.02).


CONCLUSIONS
13q14 deletion represented an early chromosome change and showed a preferential association with MCL, though it was found in virtually all principal histologic subtypes, irrespective of clinical stage, karyotype status and complexity. Patients with 13q14 deletions had a low CR rate, suggesting that genes relevant to lymphomagenesis are located in this chromosome segment that warrants molecular cytogenetic investigation.
Istituto di Ematologia, Università di Ferrara, via Savonarola 9, 44100 Ferrara, Italy. sse@dns.unife.it
A
A
Cuneo
R
R
Bigoni
G M
GM
Rigolin
M G
MG
Roberti
A
A
Bardi
D
D
Campioni
C
C
Minotto
P
P
Agostini
R
R
Milani
F
F
Bullrich
M
M
Negrini
C
C
Croce
G
G
Castoldi
D014408:Biomarkers, Tumor; D002882:Chromosomes, Human, Pair 13; D015321:Gene Rearrangement; D016161:Genes, Retinoblastoma; D006801:Humans; D007621:Karyotyping; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000503:physiopathology; D015999:Multivariate Analysis; D011379:Prognosis; D017384:Sequence Deletion
D014408:Biomarkers, Tumor