Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system.
The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before UV-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the phosphate starvation-inducible (psi) genes of E. coli.
Division of Cancer Research, National Health Research Institutes, Taipei, Taiwan, ROC. sechuang@nhri.org.tw
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Chuang
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Blattner
D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects* / Q000821:virology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics; D010710:Phosphates; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D003470:Culture Media; D010710:Phosphates; C061278:PhoB protein, Bacteria; D005993:Glycerolphosphate Dehydrogenase; C096203:glycerol-3-phosphate dehydrogenase (NAD(P)+)