Bioreactor development for tissue-engineered cartilage.
The development of tissue engineered cartilage is emerging as a potential treatment for the repair of cartilage defects. By seeding chondrocytes onto poly-glycolic acid (PGA) biodegradable scaffolds within a convective-flow bioreactor, the synthesis of tissue-engineered articular cartilage has been recently demonstrated. The ability to cultivate and manipulate this cell-polymer construct to possess specific dimensions, as well as biochemical and biomechanical properties is critical for potential application as an in vivo therapy of damaged articular surfaces. Bioreactor design requirements for stages from research to development to commercialization are discussed. Advantages and limitations to various bioreactor designs are critiqued. These studies illustrate the ability to synthesize tissue-engineered cartilage under convective-flow conditions for potential human tissue repair.
Advanced Tissue Sciences, Inc., La Jolla, California 92037-1005, USA. flo.wu@advancedtissue.com
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Wu
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Dunkelman
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Peterson
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Davisson
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De La Torre
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Jain
D019149:Bioreactors; D002356:Cartilage / Q000166:cytology / Q000254:growth & development*; D002448:Cell Adhesion; D046508:Culture Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans