Isolation of a novel microorganism, pestalotia heterocornis, producing paclitaxel
Pestalotia heterocornis was isolated from soil collected in yew forest and was shown to produce paclitaxel in semisynthetic liquid media. The presence of paclitaxel in the fungal extract was confirmed by FAB mass spectrometry and NMR spectroscopy. The maximum yield of paclitaxel was 31 &mgr;g per liter. Optimal paclitaxel production occurred after 5-7 days in a 20-liter scale fermentation at 23 degrees C. These results indicate that P. heterocornis is an excellent candidate for consideration in fermentation technology. Copyright 1999 John Wiley & Sons, Inc.
Noh
MJ
Biotechnology Team, KOLON Central Research Institute, 207-2, Mabuk-ri, Guseong-myun, Yongin City, Kyunggi-do, 449-910, Korea.
Yang
JG
Kim
KS
Yoon
YM
Kang
KA
Han
HY
Shim
SB
Park
HJ