Concentration- and time-dependent effects of gamma-linolenic acid supplementation to tumor cells in culture.
Gamma-linolenic acid (GLA) supplemented to neuroblastoma SK-N-BE, tubal carcinoma TG and colon carcinoma SW-620 cells was incorporated into phospholipids in all the cell lines (although to different extents), in a concentration- and time-dependent manner. All the cell lines were able to metabolize GLA to arachidonic acid, SK-N-BE being the most active. Supplementation with low GLA concentrations for short periods was not sufficient to impair cell proliferation; only higher amounts of GLA had an anti-proliferative effect also in short times. In these conditions, the antiproliferative effect of GLA is probably due to cellular dysfunction caused by fatty acid modifications.
Hrelia
S
S
Department of Biochemistry G. Moruzzi, University of Bologna, Italy.
Pession
A
A
Buda
R
R
Lorenzini
A
A
Horrobin
D F
DF
Biagi
P L
PL
Bordoni
A
A
D016718:Arachidonic Acid / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured; D017965:gamma-Linolenic Acid / Q000494:pharmacology*
D005227:Fatty Acids; D010743:Phospholipids; D016718:Arachidonic Acid; D017965:gamma-Linolenic Acid; D013936:Thymidine