Time-resolved analysis and visualization of dynamic processes in living cells.
Recent development of in vivo microscopy techniques, including green fluorescent proteins, has allowed the visualization of a wide range of dynamic processes in living cells. For quantitative and visual interpretation of such processes, new concepts for time-resolved image analysis and continuous time-space visualization are required. Here, we describe a versatile and fully automated approach consisting of four techniques, namely highly sensitive object detection, fuzzy logic-based dynamic object tracking, computer graphical visualization, and measurement in time-space. Systematic model simulations were performed to evaluate the reliability of the automated object detection and tracking method. To demonstrate potential applications, the method was applied to the analysis of secretory membrane traffic and the functional dynamics of nuclear compartments enriched in pre-mRNA splicing factors.
Tvaruskó
W
W
Bioinformatics Group, Interdisciplinary Center of Scientific Computing, 69120 Heidelberg, Germany.
Bentele
M
M
Misteli
T
T
Rudolf
R
R
Kaether
C
C
Spector
D L
DL
Gerdes
H H
HH
Eils
R
R
D000818:Animals; D001695:Biology / Q000379:methods; D002468:Cell Physiological Phenomena; D002522:Chlorocebus aethiops; D002864:Chromogranins / Q000032:analysis / Q000235:genetics; D003198:Computer Simulation; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013997:Time Factors; D014162:Transfection; D014709:Vero Cells
D002864:Chromogranins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins