Secretion of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 by meningiomas detected by cell immunoblot analysis.
To identify features of meningiomas that infiltrate dura mater, and to examine the role Matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) secretion in meningiomas, a cell immunoblot assay study was performed in 20 meningiomas. MMP-9 secretion was detected in 20 (100%) meningiomas and was independent of histological features. TIMP-1 secretion was detected in 8 (40%) meningiomas. For a cell immunoblot volume which included approximately 1350 cells, there were 50.4 +/- 49.4 dots which showed immunoreactivity to MMP-9 and 2.0 +/- 4.2 which showed immunoreactivity to TIMP-1. The number of dots that showed immunoreactivity to MMP-9 was not significantly different between tumours with atypical and benign histological features. TIMP-1 secretion was found in only 8 (40%) specimens and the percentage of TIMP-1 secreting cells was significantly lower than that of MMP-9 secretion. Thus, we propose that meningiomas classified histologically as both atypical and benign have the potential to infiltrate dura mater.
Department of Neurosurgery, Hiroshima Prefectural Hospital, Hiroshima University School of Medicine, Japan.
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T
Mizoue
H
H
Kawamoto
K
K
Arita
A
A
Tominaga
K
K
Kurisu
D000328:Adult; D000368:Aged; D017364:Collagenases / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry; D008297:Male; D020780:Matrix Metalloproteinase 9; D008577:Meningeal Neoplasms / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D008579:Meningioma / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D008666:Metalloendopeptidases / Q000378:metabolism*; D008875:Middle Aged; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis* / Q000378:metabolism
D019715:Tissue Inhibitor of Metalloproteinase-1; D017364:Collagenases; D008666:Metalloendopeptidases; D020780:Matrix Metalloproteinase 9