Crystallization and preliminary crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancers.
Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Paneth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crystallized using the Hampton Research Crystal Screen and SAmBA software to define the optimal crystallization protocol. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.73, b = 49.35, c = 92.15 A and one molecule in the asymmetric unit. Flash-frozen crystals diffract to 1. 78 A resolution using synchrotron radiation. A molecular-replacement solution was obtained using the human Reg/lithostathine structure and the AMoRe software.
Abergel
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Information Génétique et Structurale, CNRS UMR 1889, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20, France. chantal@igs.cnrs-mrs.fr
Chenivesse
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S
Stinnakre
M G
MG
Guasco
S
S
Bréchot
C
C
Claverie
J M
JM
Devinoy
E
E
Christa
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L
D000209:Acute-Phase Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005260:Female; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D037181:Lectins, C-Type; D008113:Liver Neoplasms / Q000737:chemistry*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000737:chemistry; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins
D000209:Acute-Phase Proteins; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037102:Lectins; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C000612141:REG3A protein, human; C490253:Reg3b protein, mouse