Bioenergetic effect of liposomal coenzyme Q10 on myocardial ischemia reperfusion injury.
The antioxidant and bioenergetic effects of CoQ10 are well known but its clinical utility is limited by the requirement for enteral administration. A newly developed liposomal CoQ10 (CoQ) is water soluble and capable of intravenous administration. The purpose of this study is to determine the mechanism by which acute administration CoQ protects myocardium from reperfusion (Rp) injury. Rats were pretreated with CoQ 10 mg/kg i.v. 30 min prior to the experiment. Control rats were pretreated with liposome only. Hearts were excised and subjected to equilibration, 25 min of normothermic ischemia and 40 min of Rp on a Langendorff apparatus. At end Rp, CoQ hearts recovered 74 +/- 5% of their DP vs. 50 +/- 9% in control (p < 0.05). Aerobic efficiency was maintained (0.66 +/- 0.02 vs. control, 0.5 +/- 0.04, p < 0.003) and CoQ hearts lost less CK activity vs. control (p < 0.02). PCr and ATP were higher than control (p < 0.05, 0.02, respectively). Results show that i.v. CoQ improves recovery of function, aerobic efficiency, CK activity, and recovery of PCr and ATP after Rp. This suggests that acute administration of liposomal CoQ improves myocardial tolerance to I/R via its role as an antioxidant as well as improving oxygen utilization and high energy phosphate production.
Department of Cardiothoracic Surgery, Allegheny University/MCP, Philadelphia, PA 19129, USA.
K
K
Niibori
K P
KP
Wroblewski
H
H
Yokoyama
J A
JA
Crestanello
G J
GJ
Whitman
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003067:Coenzymes; D003402:Creatine Kinase / Q000378:metabolism; D004337:Drug Carriers; D004734:Energy Metabolism / Q000187:drug effects*; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
D003067:Coenzymes; D004337:Drug Carriers; D008081:Liposomes; D010710:Phosphates; D010725:Phosphocreatine; D014451:Ubiquinone; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; C024989:coenzyme Q10