Inhibition of sham feeding-stimulated human gastric acid secretion by glucagon-like peptide-2.
Glucagon-like peptide (GLP)-2 is formed from proglucagon in the intestinal L cells and is secreted postprandially in parallel with the insulinotropic hormone GLP-1, the latter of which, in addition, acts to inhibit gastric secretion and motility by inhibiting central parasympathetic outflow. We now studied the effect of GLP-2 on gastric secretion stimulated by sham feeding to test the hypothesis that also GLP-2 acts as an enterogastrone. Eight healthy volunteers were studied twice on separate days. They were sham fed with and without GLP-2 infused iv at a rate of 0.8 pmol/kg x min. Gastric contents were aspirated continuously by a nasogastric tube for determination of acid secretion, volume, and osmolarity. Sham feeding increased gastric acid secretion nearly 5-fold. Infusion of GLP-2 reduced incremental acid secretion by 65+/-6%, compared with saline infusion (delta8.75+/-0.37 vs. delta3.04+/-0.47 mmol x 60 min; P<0.01). Plasma concentrations of GLP-2 rose from a basal mean of 3.3+/-0.9 to a mean of 115+/-8 pmol/L (range, 57-149 pmol/L) during infusion of GLP-2 and remained at basal level during saline infusion. Plasma concentrations of GLP-1, gastrin, cholecystokinin, and secretin remained low and unchanged on both study days. We conclude that GLP-2 is a powerful inhibitor of gastric acid secretion in man. Further investigations will show to what extent GLP-2 contributes to the inhibitory effects on gastric secretion exerted by hormones from the distal small intestine, under physiological circumstances.
Department of Surgery, Rigshospitalet, Copenhagen, Denmark.
M
M
Wøjdemann
A
A
Wettergren
B
B
Hartmann
L
L
Hilsted
J J
JJ
Holst
D002766:Cholecystokinin / Q000097:blood; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000097:blood; D005768:Gastrointestinal Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D005934:Glucagon / Q000097:blood; D052216:Glucagon-Like Peptide 1; D053767:Glucagon-Like Peptide 2; D006801:Humans; D007262:Infusions, Intravenous; D010446:Peptide Fragments / Q000097:blood; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D011498:Protein Precursors / Q000097:blood; D012633:Secretin / Q000097:blood
D005755:Gastrins; D005768:Gastrointestinal Hormones; D053767:Glucagon-Like Peptide 2; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D012633:Secretin; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D002766:Cholecystokinin