[A trial of rapid assay of identification and susceptibility test of bacteria detected from blood culture by using VITEK AMS].
For rapid identification and susceptibility test of bacteria detected from bottles of blood culture, we tried a direct method that adjusted bacterial fluid by one time centrifugalization. Identification and susceptibility test were done using VITEK AMS. A result of having compared direct method with a standard method inspected from a colony of medium, an agreement rate was 82.1% of gram negative bacilli, 64.0% of gram positive cocci, 100% of yeast in identification test. In the same way, an agreement rate was 98.0% of gram negative bacilli, 97.2% of gram positive cocci in susceptibility test. The appearance rate of very major error was 0. 7% in gram negative bacilli, and 2.8% in gram positive cocci. As for this method, operation is simple, but it is necessary for confirmed examination in a kind of bacteria. But agreement rate with a standard method is high, direct method is useful to select an appropriate antibiotic until reporting of the last test result of bacteria.
Division of Clinical Microbiology, Department of Medical Technology, Kinki University School of Medicine, Ohno-Higashi 377-2, Osakasayama, Osaka 589-8511, Japan.
K
K
Satoh
S
S
Kubo
K
K
Matsushita
T
T
Akiyama
I
I
Furuta
D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D005658:Fungi / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006095:Gram-Positive Cocci / Q000302:isolation & purification; D006801:Humans; D013997:Time Factors
D003470:Culture Media