A mutational study of a Diels-Alderase catalytic antibody.
Site-directed mutagenesis was used to probe the mechanism of antibody 39-A11, which catalyzes the Diels-Alder reaction of substrate 1 and 2 to give cycloadduct 3. Mutations that lead to improved packing interactions with the diene afford an order of magnitude increase in catalytic activity.
Howard Hughes Medical Institute and the Department of Chemistry, University of California, Berkeley 94720-1460, USA.
F E
FE
Romesberg
P G
PG
Schultz
D017151:Antibodies, Catalytic / Q000737:chemistry*; D018360:Crystallography, X-Ray; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed
D017151:Antibodies, Catalytic