Alkaline single cell gel electrophoresis and human biomonitoring for genotoxicity: a study on subjects with residential exposure to radon.
Based on theoretical estimates and various correlation studies, it has been suggested that ingestion of radon in drinking water represents an increased risk for cancer. Such a risk has never been conclusively shown in epidemiological or experimental animal studies, however, and it has been questioned whether the radon level in the drinking water is of any significance in terms of overall radon exposure. Using primary DNA damage as a biological marker for an ongoing exposure to ionising radiation, the present study was undertaken to investigate whether people with different types of residential radon exposures differed with regard to their levels of DNA damage in circulating lymphocytes. DNA damage was measured in coded blood samples from 125 residents living in 45 households with different levels of radon-222 in the drinking water (10-2410 Bq/l) and indoor air (35-1025 Bq/m3) using alkaline single cell gel electrophoresis (the 'Comet' assay). Increased levels of radon in indoor air (>200 Bq/m3) were found to be associated with an increased level of DNA damage in peripheral lymphocytes (P</=0.05). No such correlation was seen for the radon concentrations in the drinking water, and there was no obvious relationship between the radon levels in drinking water and in indoor air. The results of the present study suggest that measures taken to reduce residential radon exposures should be focused on reducing radon levels in indoor air rather than minimising radon levels in drinking water.
Department of Occupational and Environmental Medicine, University Hospital, S-751 85, Uppsala, Sweden. bjorn.hellman@occmed.uu.se
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Hellman
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Friis
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Vaghef
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Edling
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000396:Air Pollutants, Radioactive / Q000009:adverse effects*; D000818:Animals; D002274:Carcinogens, Environmental / Q000009:adverse effects*; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007223:Infant; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009152:Mutagenicity Tests / Q000379:methods; D011834:Radiation Monitoring / Q000379:methods*; D011886:Radon / Q000009:adverse effects*; D014875:Water Pollutants, Radioactive / Q000009:adverse effects*; D014881:Water Supply / Q000032:analysis
D000396:Air Pollutants, Radioactive; D002274:Carcinogens, Environmental; D014875:Water Pollutants, Radioactive; D004247:DNA; D011886:Radon