Protective effects of Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP), a superoxide dismutase mimetic, in paw oedema induced by carrageenan in the rat.
In the present study we investigated the therapeutic efficacy of Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP), a superoxide dismutase mimetic which possesses peroxynitrite scavenging effects, in rats subjected to carrageenan-induced paw oedema. Local administration of MnTBAP (5, 25, and 50 microg/paw) significantly and dose dependently reduced carrageenan-induced paw oedema at all time points. MnTBAP also caused a significant dose-dependent reduction in paw myeloperoxidase activity and lipid peroxidation, as well as preventing histological injury. Immunohistochemical analysis for nitrotyrosine revealed a positive staining in paw from carrageenan-treated rats. No positive nitrotyrosine staining was found in the paws of the carrageenan-treated rats that received MnTBAP. Our study demonstrates that MnTBAP exerts protective effects in carrageenan-induced paw oedema. Part of these anti-inflammatory effects may be related to: 1) reduction of superoxide formation due to the superoxide dismutase-like activity of the compound; and 2) scavenging of peroxynitrite.
Cuzzocrea
S
S
Institute of Pharmacology, University of Messina, Italy. salvator@imeuniv.unime.it
Costantino
G
G
Mazzon
E
E
Zingarelli
B
B
De Sarro
A
A
Caputi
A P
AP
D000818:Animals; D002351:Carrageenan; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008297:Male; D008665:Metalloporphyrins / Q000627:therapeutic use*; D018716:Molecular Mimicry; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D016166:Free Radical Scavengers; D008665:Metalloporphyrins; D020011:Protective Agents; C097284:manganese(III)-tetrakis(4-benzoic acid)porphyrin; D002351:Carrageenan; D009195:Peroxidase; D013482:Superoxide Dismutase