Development and viability of bovine preplacentation embryos treated with swainsonine in vitro.
This study investigated the effects of swainsonine (a locoweed toxin) on bovine preplacentation embryo development using in vitro procedures. We examined and confirmed the viability and developmental potential of swainsonine-treated embryos by transfer to synchronized recipient heifers. Oocytes (n = 6338) were aspirated from ovaries collected from the abattoir and subjected to in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). Swainsonine was added to IVM, IVF, IVC media spatially and IVM/IVF/IVC continuously, at 0 ng/ml (TRTI, control), 200 ng/ml (TRT2), 400 ng/ml (TRT3), and 800 ng/ml (TRT4). Embryo development was evaluated with respect to oocyte cleavage rate and the rates of morula and blastocyst formation. There was no difference (P > 0.05) among treatments. The average number of nuclei per blastocyst at Day 7.5 of culture (Day 0 = IVF) was 85.9 +/- 4.3 (n = 47) and 89.3 +/- 4.4 (n = 44) for swainsonine-treated embryos (800 ng/ml) and control embryos, respectively. Pregnancy rate as determined by ultrasonography on day 35 to 40 post embryo transfer was 43.8% and 38.3% for swainsonine-treated (800 ng/ml) and control embryos, respectively. Nine (9.4%) healthy calves were delivered from heifers receiving swainsonine-exposed and nine (9.6%) from control embryos. No difference (P > 0.05) was detected in number of calves developing from TRT and control embryos. We conclude that swainsonine does not have an adverse effect on the development and viability of preplacentation bovine embryos.
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-9400, USA. swang@cc.usu.edu
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Wang
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Panter
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Holyoak
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Molyneux
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Evans
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D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D002452:Cell Count / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary; D004791:Enzyme Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary; D017026:Swainsonine / Q000494:pharmacology* / Q000633:toxicity
D004791:Enzyme Inhibitors; D017026:Swainsonine