Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers.
We have transformed potato with Nt-inhh cDNA, encoding a putative vacuolar homolog of a tobacco cell wall invertase inhibitor, under the control of the CaMV 35S promoter. In transgenic tubers, cold-induced hexose accumulation was reduced by up to 75%, without any effect on potato tuber yield. Processing quality of tubers was greatly improved without changing starch quantity or quality, an important prerequisite for the biotechnological use of Nt-inhh for potato transformation.
Greiner
S
S
Botanisches Institut, INF 360, Heidelberg, Germany.
Rausch
T
T
Sonnewald
U
U
Herbers
K
K
D000595:Amino Acid Sequence; D001709:Biotechnology; D002473:Cell Wall / Q000201:enzymology; D003001:Cloning, Molecular; D003080:Cold Temperature; D004791:Enzyme Inhibitors; D015971:Gene Expression Regulation, Enzymologic; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic; D014617:Vacuoles / Q000201:enzymology; D043324:beta-Fructofuranosidase
D004791:Enzyme Inhibitors; D006601:Hexoses; C110996:INH protein, Nicotiana tabacum; D010940:Plant Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase