Expression and immunogenicity of a mutant diphtheria toxin molecule, CRM(197), and its fragments in Salmonella typhi vaccine strain CVD 908-htrA.
Mutant diphtheria toxin molecule CRM(197) and fragments thereof were expressed in attenuated Salmonella typhi CVD 908-htrA, and the constructs were tested for their ability to induce serum antitoxin. Initially, expressed proteins were insoluble, and the constructs failed to induce neutralizing antitoxin. Soluble CRM(197) was expressed at low levels by utilizing the hemolysin A secretion system from Escherichia coli.
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Department of Pediatrics, Division of Infectious Diseases and Tropical Pediatrics, Center for Vaccine Development, Department of Medicine, Division of Geographic Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Galen
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Levine
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MM
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D004167:Diphtheria Toxin / Q000276:immunology*; D029968:Escherichia coli Proteins; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012485:Salmonella typhi / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000276:immunology*
D001426:Bacterial Proteins; D004167:Diphtheria Toxin; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C028581:CRM197 (non-toxic variant of diphtheria toxin)