Copper-zinc superoxide dismutase from the marine yeast Debaryomyces hansenii.
We have isolated the cytosolic form of Cu-Zn superoxide dismutase (SOD) from the marine yeast Debaryomyces hansenii. This enzyme has a subunit mass of 18 kDa. The preparation was found to be heterogeneous by IF electrophoresis with two pI ranges: 5.14-4.0 and 1.6-1.8. The enzyme preparation had a remarkably strong stability at pH 6.0-7.0, surviving boiling for 10 min without losing more than 60% of activity. On Western blots, this enzyme was recognized by antibodies raised in rabbits against D. hansenii extracts, while only a weak cross-reaction could be detected using antibodies generated against either Saccharomyces cerevisiae or bovine erythrocyte Cu-Zn SODs. In sequencing analysis, a peptide obtained by trypsin digestion was found to have 85% identity to the S. cerevisiae Cu-Zn SOD.
Hernández-Saavedra
N Y
NY
Center for Biological Research, Northwest, Laboratory of Marine Yeast, Baja California Sur, México. nhernan@cibnor.mx
Ochoa
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal; D000918:Antibody Specificity; D015153:Blotting, Western; D002417:Cattle; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D011817:Rabbits; D012623:Seawater / Q000382:microbiology; D017421:Sequence Analysis; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D015003:Yeasts / Q000201:enzymology*
D000908:Antibodies, Fungal; D005656:Fungal Proteins; D013482:Superoxide Dismutase