Production of a diagnostic monoclonal antibody in perennial alfalfa plants.
The increasing use of monoclonal antibodies (mAbs) in diagnostic reagents necessitates efficient and cost-effective mAb production methods. In blood banks, one of the most routinely used reagents is the anti-human IgG reagent used for the detection of non-agglutinating antibodies. Here we report the production of a functional, purified anti-human IgG, through the expression of its encoding genes in perennial transgenic alfalfa. Transgenic plants expressing the light- and heavy-chain encoding mRNAs were obtained, and plants from crosses were found to express fully assembled C5-1. The purification procedure yielded mainly the H2L2 form with specificity and affinity identical to those of hybridoma-derived C5-1. The ability to accumulate the antibody was maintained both in parental F1 lines during repeated harvesting and in clonal material; the antibody was stable in the drying hay as in extracts made in pure water. Also, plant and hybridoma-derived C5-1 had similar in vivo half-lives in mice. These results indicate that plant C5-1 could be used in a diagnostic reagent as effectively as hybridoma-derived C5-1, and demonstrates the usefulness of perennial systems for the cost-effective, stable, and reliable production of large amounts of mAbs.
Plant Science Department, FSAA, Université Laval, Sainte-Foy, Québec G1K 7P4.
H
H
Khoudi
S
S
Laberge
J M
JM
Ferullo
R
R
Bazin
A
A
Darveau
Y
Y
Castonguay
G
G
Allard
R
R
Lemieux
L P
LP
Vézina
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002846:Chromatography, Affinity; D017343:Genes, Plant; D006801:Humans; D006825:Hybridomas; D000455:Medicago sativa / Q000235:genetics* / Q000276:immunology; D051379:Mice; D030821:Plants, Genetically Modified; D013997:Time Factors
D000911:Antibodies, Monoclonal