Conservation of expression and N-terminal sequences of the Pasteurella haemolytica 31-kilodalton and Pasteurella trehalosi 29-kilodalton periplasmic iron-regulated proteins.
This study examined the conservation of expression of a 31-kDa iron-regulated protein by serotypes of Pasteurella haemolytica and Pasteurella trehalosi associated with pasteurellosis of cattle and sheep. A polyclonal antibody prepared against the purified 31-kDa periplasmic iron-regulated protein from P. haemolytica serotype A1 showed that all P. haemolytica serotypes expressed similar 31-kDa proteins with identical N-terminal sequences, whereas P. trehalosi serotypes expressed immunologically different 29-kDa proteins with a different N-terminal sequence. Antibody to the 31-kDa iron-regulated protein was a useful tool to distinguish similarities and differences of the iron-regulated proteins of P. haemolytica and P. trehalosi.
National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA. lbt@iastate.edu
L B
LB
Tabatabai
G H
GH
Frank
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D002417:Cattle; D002712:Chlorides; D017124:Conserved Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000494:pharmacology; D015151:Immunoblotting; D007501:Iron / Q000494:pharmacology; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics*; D016978:Mannheimia haemolytica / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics*; D009997:Osmotic Pressure; D010325:Pasteurella / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate
D001426:Bacterial Proteins; D002712:Chlorides; D005290:Ferric Compounds; D008074:Lipoproteins; D008565:Membrane Proteins; C083817:Plp1 protein, Pasteurella haemolytica; D012967:Sodium Dodecyl Sulfate; D007501:Iron; C024555:ferric chloride