Interference of linoleic acid fraction in some receptor binding assays.
An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibitor. This "false positive"effect also extended to some other binding assays.
Division of Pharmacognosy and Division of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands. ingkanin@chem.leidenuniv.nl
K
K
Ingkaninan
J K
JK
von Frijtag Drabbe Künzel
A P
AP
IJzerman
R
R
Verpoorte
D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D019787:Linoleic Acid / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D058915:Purinergic P1 Receptor Antagonists; D011869:Radioligand Assay; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism; D018047:Receptors, Purinergic P1 / Q000378:metabolism*
D007202:Indicators and Reagents; D010936:Plant Extracts; D058915:Purinergic P1 Receptor Antagonists; D011957:Receptors, Opioid; D018047:Receptors, Purinergic P1; D019787:Linoleic Acid