Initial aerobic power does not alter muscle metabolic adaptations to short-term training.
To investigate the hypothesis that training-induced increases in muscle mitochondrial potential are not obligatory to metabolic adaptations observed during submaximal exercise, regardless of peak aerobic power (VO(2 peak)) of the subjects, a short-term training study was utilized. Two groups of untrained male subjects (n = 7/group), one with a high (HI) and the other with a low (LO) VO(2 peak) (means +/- SE; 51.4 +/- 0.90 vs. 41.0 +/- 1.3 ml. kg(-1). min(-1);P < 0.05), cycled for 2 h/day at 66-69% of VO(2 peak) for 6 days. Muscle tissue was extracted from vastus lateralis at 0, 3, and 30 min of standardized cycle exercise before training (0 days) and after 3 and 6 days of training and analyzed for metabolic and enzymatic changes. During exercise after 3 days of training in the combined HI + LO group, higher (P < 0.05) concentrations (mmol/kg dry wt) of phosphocreatine (40.5 +/- 3.4 vs. 52.2 +/- 4.2) and lower (P < 0.05) concentrations of P(i) (61.5 +/- 4.4 vs. 53.3 +/- 4.4), inosine monophosphate (0.520 +/- 0.19 vs. 0.151 +/- 0.05), and lactate (37.9 +/- 5.5 vs. 22.8 +/- 4.8) were observed. These changes were also accompanied by reduced levels of calculated free ADP, AMP, and P(i). All adaptations were fully expressed by 3 min of exercise and by 3 days of training and were independent of initial VO(2 peak) levels. Moreover, maximal activity of citrate synthase, a measure of mitochondrial capacity, was only increased with 6 days of training (5.71 +/- 0.29 vs. 7.18 +/- 0.37 mol. kg protein(-1). h(-1); P < 0. 05). These results demonstrate that metabolic adaptations to prolonged exercise occur within the first 3 days of training and during the non-steady-state period. Moreover, neither time course nor magnitude of metabolic adaptations appears to depend on increases in mitochondrial potential or on initial aerobic power.
Green
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Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
Grant
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Bombardier
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Ranney
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D
D000222:Adaptation, Physiological / Q000502:physiology*; D000328:Adult; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D010710:Phosphates / Q000378:metabolism; D010806:Physical Education and Training; D010807:Physical Endurance / Q000502:physiology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
D010710:Phosphates; D013385:Succinate Dehydrogenase; D002950:Citrate (si)-Synthase