The effects of the extension of transit times on qualitative and quantitative bacterial culture after clinical sampling.
The effects of the extension of transit times on qualitative and quantitative bacterial culture after clinical sampling were investigated with the clinical samples, using the bacterial flora from the cancer portion inside the uterine cervix in 25 patients with the uterine cervical cancer. In the qualitative bacterial study, the strains of both aerobic and anaerobic bacteria detected were rather preserved for 6 hours after clinical sampling and decreased in a time-dependent manner in the samples of more than 6 hours after clinical sampling. In particular, number of anaerobic bacterial species detected remarkably decreased in the samples of more than 12 hours after clinical sampling. Therefore, prompt bacterial culture after clinical sampling, possibly within 6 hours after clinical sampling, may be crucial in order to detect most probable pathogenic anaerobic bacteria, particularly when anaerobic infection is suspected. In the quantitative bacterial study, the quantity of bacteria detected were rather preserved for 3 hours after clinical sampling and decreased in a time-dependent manner in the samples of more than 3 hours after clinical sampling. Therefore, quantitative bacterial culture should be performed within 3 hours after clinical sampling.
Department of Obstetrics and Gynecology, School of Medicine, Gifu University, 40 Tsukasa-machi, Gifu city, Gifu 500-8705, Japan. mikamo@cc.gifu-u.ac.jp
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H
Mikamo
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Sato
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Y
Hayasaki
Y X
YX
Hua
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Tamaya
D000328:Adult; D000368:Aged; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D008875:Middle Aged; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
D003470:Culture Media