Scientific basis of a matrix metalloproteinase-8 specific chair-side test for monitoring periodontal and peri-implant health and disease.
Matrix metalloproteinases (MMPs), especially collagenase-2 (MMP-8), are key mediators of irreversible tissue destruction associated with periodontitis and peri-implantitis. MMP-8 is known to exist in elevated amounts and in active form in the gingival crevicular fluid (GCF) and peri-implant sulcular fluid (PISF) from progressing periodontitis and peri-implantitis lesions and sites, respectively. (Sorsa et al. Ann. N.Y. Acad. Sci. 737: 112-131 [1994]; Teronen et al. J. Dent. Res. 76: 1529-1537 [1997]). We have developed monoclonal antibodies to MMP-8 (Hanemaaijer et al. J. Biol. Chem. 272: 31504-31509 [1997]) that can be used in a chair-side dipstick test to monitor the course and treatment of periodontitis and peri-implantitis. Monoclonal and polyclonal antibody tests for MMP-8 coincided with the classical functional collagenase activity test from GCF and PISF (Sorsa et al. J. Periodont. Res. 22: 386-393 [1988]) in periodontal and peri-implant health and disease. In future a chair-side functional and/or immunological MMP-test can be useful to diagnose and monitor periodontal and peri-implant disease and health.
Department of Periodontology, University of Helsinki, Finland. tsorsa@hammas.helsinki.fi
T
T
Sorsa
P
P
Mäntylä
H
H
Rönkä
P
P
Kallio
G B
GB
Kallis
C
C
Lundqvist
D F
DF
Kinane
T
T
Salo
L M
LM
Golub
O
O
Teronen
S
S
Tikanoja
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D017364:Collagenases / Q000032:analysis* / Q000378:metabolism; D015921:Dental Implants / Q000009:adverse effects*; D005881:Gingiva / Q000201:enzymology*; D006801:Humans; D020784:Matrix Metalloproteinase 8; D008991:Monitoring, Physiologic / Q000379:methods*; D010510:Periodontal Diseases / Q000175:diagnosis* / Q000201:enzymology / Q000628:therapy; D010518:Periodontitis / Q000175:diagnosis* / Q000201:enzymology / Q000209:etiology / Q000628:therapy; D010519:Periodontium / Q000187:drug effects*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015921:Dental Implants; D017364:Collagenases; D020784:Matrix Metalloproteinase 8