Automated system for capture and detection of nucleic acids.
A fully automated nucleic acid analysis system is described, which offers positive sample identification, improved sensitivity and reduced user interaction compared to conventional techniques. The system relies on the sequence-specific capture of DNA onto solid-phase particles, confirming product identity without the problems of interpretation and lack of sequence information inherent in gel-based analyses. The system can be used for sequence confirmation, mutation analysis and semiquantitative detection of PCR products.
Brown
A
A
Tepnel Life Sciences PLC, Manchester, England, UK.
Akinsanya
A A
AA
Barker
S J
SJ
Brophy
M
M
Dobb
A K
AK
Doyle
S M
SM
Hudson
I R
IR
Minter
S J
SJ
Wraith
M J
MJ
Oultram
J D
JD
D001331:Automation; D019921:Biotinylation; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D008863:Microspheres; D009696:Nucleic Acids / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017421:Sequence Analysis / Q000379:methods
D017931:DNA Primers; D009696:Nucleic Acids; D009841:Oligonucleotides