Reduction of bioactive substances in stored donor blood: prestorage versus bedside leucofiltration.
Leucocyte filtration has been suggested to improve transfusion products. We studied the effect of prestorage versus bedside leucofiltration on reduction of bioactive substances and leucocyte content in donor blood. Forty-five units of whole blood from healthy blood donors were studied. Of these units, 9 were stored under standard conditions for 35 d, 9 were leucofiltered after donation and then stored for 35 d, and 3x9 units were stored for 7, 21 and 35 d, respectively, before leucofiltration. Samples were collected from blood units immediately after donation, and before and after leucofiltration, and analysed by ELISA and RIA methods for extracellular content of myeloperoxidase (MPO), eosinophil cationic protein (ECP), histamine (HIS) and plasminogen activator inhibitor-1 (PAI-1). Leucocyte content was counted in all samples. In non-filtered blood extracellular MPO, ECP, HIS and PAI-1 were accumulated in a storage time-dependent manner, while prestorage leucofiltration prevented this accumulation. Leucofiltration after storage for 7, 21 or 35 d did not significantly reduce the accumulated bioactive substances, which were similar to levels in non-filtered blood stored for the same period of time. Prestorage and bedside leucofiltration on day 7 reduced the leucocyte content to less than 0.5x10(6)/L, whereas the median content in blood stored for 21 or 35 d was only reduced to 32.0 and 52.2x10(6)/L, respectively. Prestorage leucofiltration may thus be advantageous to bedside leucofiltration.
Department of Surgical Gastroenterology, Hvidovre Hospital, University of Copenhagen, Denmark. j.h.hammer@forum.dk
J H
JH
Hammer
T
T
Mynster
C M
CM
Reimert
A N
AN
Pedersen
H J
HJ
Nielsen
D001782:Blood Donors; D001798:Blood Proteins / Q000032:analysis; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay; D047091:Eosinophil Granule Proteins; D006440:Hemofiltration / Q000379:methods*; D006632:Histamine / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D009195:Peroxidase / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D011863:Radioimmunoassay; D012260:Ribonucleases; D013997:Time Factors
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D017395:Plasminogen Activator Inhibitor 1; D006632:Histamine; D009195:Peroxidase; D012260:Ribonucleases