Sequence of HLA-A*6808.
A novel HLA-A*68 allele was identified by reference strand conformation analysis of a DNA sample studied for the International Cell Exchange program. Direct sequencing of HLA-A locus PCR products confirmed the presence of A*6602 and an A*68 allele which differed from A*68011 by two nucleotides at positions 538 and 539. The presence of this sequence motif in this allele which has been named A*6808 was verified by DNA sequencing of full-length A*6808 clones. No other coding differences between A*68011 and A*6808 were identified. The two nucleotide substitutions found in A*6808 effect an amino acid difference in the encoded protein at residue 156 within the peptide binding site which may evoke alloreactive immune response after HLA-A68 mismatched transplants.
Anthony Nolan Research Institute, The Royal Free Hospital, Hampstead, London, UK.
S T
ST
Cox
J R
JR
Argüello
S G
SG
Marsh
M
M
Lau
P L
PL
Kwan
J A
JA
Madrigal
A M
AM
Little
D044383:Black People / Q000235:genetics; D015234:HLA-A Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data
D015234:HLA-A Antigens; C121607:HLA-A*68:08 antigen