Synergism between mild hyperthermia and interferon-beta gene expression.
In the present study, the synergistic effect of mild hyperthermia in combination with gene expression of interferon-beta (IFN-beta) was examined in vitro in the human glioma cell line U87MG. The cells transiently expressed the IFN-beta gene under the control of the mouse mammary tumor virus promoter and were then subjected to temperature elevation (41 degrees C for 1 h). In terms of the cell killing effect, the optimum scheme was obtained by transfection for 4 days before hyperthermia, i.e. rate in the time course of IFN-beta gene expression. The relative specific growth rate decreased to 32% compared with the control while it was only 40% under the hyperthermic conditions. These observations suggest that IFNbeta expression was able to enhance the sensitivity of transfected glioma cells to mild hyperthermia.
Bouhon
I A
IA
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Japan.
Shinkai
M
M
Honda
H
H
Mizuno
M
M
Wakabayashi
T
T
Yoshida
J
J
Kobayashi
T
T
D000818:Animals; D016923:Cell Death; D002455:Cell Division; D003131:Combined Modality Therapy; D015870:Gene Expression; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D006801:Humans; D006979:Hyperthermia, Induced; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D016899:Interferon-beta