Meta-tetra(hydroxyphenyl)chlorin-sensitized photodynamic damage of cultured tumor and normal cells in the presence of high concentrations of alpha-tocopherol.
Alpha-tocopherol at low concentrations protects biosubstrates from oxidative damage, while at high concentrations it may become toxic. Certain lines of tumor cells are reported to contain higher levels of vitamin E than normal cells. In our study alpha-tocopherol was successfully incorporated by cultured HT29 adenocarcinoma cells, but not by MRC-5 normal fibroblasts. At high concentrations (0.3-1 mM) alpha-tocopherol enhanced meta-tetra(hydroxyphenyl)chlorin (mTHPC)-sensitized photoinactivation of HT29 cells (415 nm), but not that of normal fibroblasts. At none of the concentrations used (0.001-1 mM) did alpha-tocopherol protect cells from photokilling, indicating that lipid peroxidation is of minor importance in mTHPC photoactivity. Our findings encourage the in vivo testing of phenolic antioxidants for selective enhancement of PDT-damage in tumors.
Unité de Recherche en Thérapie Photodynamique, Centre Alexis Vautrin, Vandoeuvre-les-Nancy, France.
V
V
Melnikova
L
L
Bezdetnaya
I
I
Belitchenko
A
A
Potapenko
J L
JL
Merlin
F
F
Guillemin
D000230:Adenocarcinoma / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D008652:Mesoporphyrins / Q000494:pharmacology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013997:Time Factors; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D014810:Vitamin E; C072269:temoporfin