Evidence of carbamoylphosphate induced conformational changes upon binding to human ornithine carbamoyltransferase.
Human liver ornithine carbamoyltransferase undergoes absorbance changes in the UV region upon formation of the carbamoylphosphate-norvaline-enzyme ternary complex. The UV changes are similar in the presence of carbamoylphosphate alone, whilst they are lower in the presence of ornithine or norvaline alone. The extent of the UV changes correlates with the enzyme susceptibility to proteolytic degradation. The free native enzyme is completely and rapidly hydrolyzed by trypsin, whilst it is partially protected upon carbamoylphosphate binding. The extent of protection increases for the carbamoylphosphate-norvaline-enzyme ternary complex. These results strongly suggest that the binding of the first substrate, i.e. carbamoylphosphate, to human ornithine carbamoyltransferase induces a large protein isomerization, which regards the polar domain plus a part of equatorial domain of each subunit.
Dipartimento di Chimica Organica e Biologica, Università di Messina, Italy. adegre@isengard.unime.it
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De Gregorio
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Risitano
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Capo
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Criniò
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Petruzzelli
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Desideri
D002221:Carbamyl Phosphate / Q000494:pharmacology*; D004795:Enzyme Stability; D006801:Humans; D008099:Liver / Q000201:enzymology; D009952:Ornithine / Q000737:chemistry; D009954:Ornithine Carbamoyltransferase / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin / Q000378:metabolism; D014633:Valine / Q000031:analogs & derivatives / Q000737:chemistry
D002221:Carbamyl Phosphate; C005313:norvaline; D009952:Ornithine; D009954:Ornithine Carbamoyltransferase; D014357:Trypsin; D014633:Valine