Identification of a novel HLA-DQA1 allele (DQA1*0106) by sequence-based DQA1 typing.
We report here a novel DQA1 allele (DQA1*0106) identified during sequence-based HLA-DQA1 typing. Polymerase chain reaction with proofreading pfu DNA polymerase and subsequent sequencing yielded identical results as that with Taq DNA polymerase. Molecular cloning and sequencing confirmed that the new DQA1 allele is identical to DQA1*01021/2 at exon 2 except for a single nucleotide substitution (ACT-->GCT), changing codon 44 from Thr to Ala. This is the first report of polymorphism at codon 44 of HLA-DQA1 alleles.
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
M
M
Luo
J
J
Blanchard
I
I
Maclean
R
R
Brunham
D000409:Alanine; D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013912:Threonine
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D013912:Threonine; D000409:Alanine