Evaluation of teflon-coated intraocular lenses in an organ culture method.
An amorphous and transparent form of Teflon is proposed as a coating of polymethylmethacrylate (PMMA) intraocular lenses (IOLs), rendering them highly hydrophobic. We used an organ culture method to evaluate cell adhesion, proliferation, and migration on Teflon-coated IOLs. Corneal explants from 14-day-old chicken embryos were placed on a semisolid culture medium and covered with uncoated PMMA (n = 36) and Teflon-coated PMMA (n = 36) IOLs and two controls, Thermanox (n = 84) and latex (n = 36). After incubation (7 days at 37 degrees C), a digital imaging system was used to measure the areas of the cell migration layers on the materials. The cells were then removed with tripsin-ethylenediaminetetraacetic acid and the cells detached at times up to 75 min were counted (Coulter(R) Multisizer System). The values were used to construct a cell disconnecting curve for each material. The areas of cell migration layers on uncoated and Teflon-coated IOLs were significantly different (p <.05). Cell disconnecting curves demonstrated that cells adhered less strongly to Teflon-coated IOLs than to the other materials. This organ culture method demonstrated that the coating of PMMA IOLs with Teflon AF(R) is correlated with antiadhesive and antiproliferative properties.
Department of Ophthalmology, Hôtel-Dieu Hospital, and Laboratoire de Recherche sur les Therapeutiques Substitutives en Ophtalmologie (EA 2395, CRI 9808), 1, place du Parvis-Notre-Dame, F-75181, Paris Cedex 04, France.
L
L
Werner
J M
JM
Legeais
M D
MD
Nagel
G
G
Renard
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002642:Chick Embryo; D003315:Cornea / Q000166:cytology; D005069:Evaluation Studies as Topic; D019654:Lens Implantation, Intraocular; D008422:Materials Testing; D009924:Organ Culture Techniques; D019904:Polymethyl Methacrylate; D011138:Polytetrafluoroethylene; D013499:Surface Properties
D011138:Polytetrafluoroethylene; D019904:Polymethyl Methacrylate