Enterococcus hirae vacuolar ATPase is expressed in response to pH as well as sodium.
The Enterococcus hirae ntp operon encodes both a vacuolar ATPase, which transports Na+ as well as Li+, and the KtrII K+ transporter. A plasmid, in which the chloramphenicol acetyltransferase gene (CAT) was placed downstream of the ntp promoter, was introduced into a mutant totally defective in Na+ extrusion. The CAT activity of this transformant was increased preferentially by addition of NaCl, but not by LiCl, in the media or by elevating the medium pH, correlating well with the increase in amounts of the ATPase subunits observed by Western blotting. The physiological significance of these responses of the ntp promoter is discussed.
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
M
M
Ikegami
M
M
Kawano
K
K
Takase
I
I
Yamato
K
K
Igarashi
Y
Y
Kakinuma
D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016983:Enterococcus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008094:Lithium / Q000378:metabolism; D008957:Models, Genetic; D011188:Potassium / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013997:Time Factors; D025262:Vacuolar Proton-Translocating ATPases
D008094:Lithium; D012964:Sodium; D015500:Chloramphenicol O-Acetyltransferase; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D011188:Potassium