Expression of an antigen associated with basal bodies of human ciliated epithelial cells.
The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M(r) protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9 + 0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.
Comer
M T
MT
CRF Cancer Medicine Research Unit, St James's University Hospital, Leeds, UK.
Shires
M
M
Goode
N P
NP
Leese
H J
HJ
Trejdosiewicz
L K
LK
Southgate
J
J
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000941:Antigens / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D002923:Cilia / Q000737:chemistry* / Q000276:immunology; D004847:Epithelial Cells / Q000737:chemistry* / Q000276:immunology; D005187:Fallopian Tubes / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000276:immunology* / Q000648:ultrastructure; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000941:Antigens