[Experimental autoimmune uveitis. Characterization of retina infiltrating cells].
The chronic model of murine EAU induced by interphotoreceptor retinoid binding protein represents a disease similar to clinical chorioretinitis. In this study we characterized the kinetics of retina infiltrating T-cells, macrophages and expression of the adhesion molecules ICAM-1 and ICAM-2.


METHODS
B10.A mice were immunized subcutaneously with IRBP, and the eyes were analyzed on days 10, 18, 24 and 28. The infiltrating cells were characterized by mAbs recognizing T-cell receptors (TCR) Vss6 and Vss8, T-cell markers, macrophages and ICAM-1 and ICAM-2.


RESULTS
While CD8+ T-cells and ICAM-2 were detectable from day 10 (retina is intact) until day 28, CD4+ T-cells, macrophages and ICAM-1 appear with the onset of retinal destruction. Starting at day 10 the dominating TCR was Vss6; Vss8 was noticed from day 18 on.


CONCLUSIONS
CD8+ T-cells infiltrating the intact retina and stimulating the expression of high endothelial venules (HEVs) could be responsible for the onset of uveitis.
Zierhut
M
M
Abteilung I, Augenklinik der Universität, Tübingen.
Wild
U
U
Roser
R
R
Wiggert
B
B
Thiel
H J
HJ
Stiemer
R
R
D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001327:Autoimmune Diseases / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000032:analysis; D002825:Chorioretinitis / Q000276:immunology*; D005136:Eye Proteins; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D012160:Retina / Q000276:immunology; D012177:Retinol-Binding Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014605:Uveitis / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D005136:Eye Proteins; D018799:Intercellular Adhesion Molecule-1; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial; C065289:ICAM2 protein, human
Experimentelle Autoimmun-Uveitis. Charakterisierung der die Retina infiltrierenden Zellen.