Increase production of lactonizing lipase (LipL) from Pseudomonas sp. strain 109 by lipids and detergents.
LipL of Pseudomonas sp. strain 109 is a unique lipase capable of catalyzing macrocyclic lactone synthesis using omega-hydroxyfatty acid esters as substrates. Several fatty acid esters were tested as inducers of LipL production. The addition of either soybean oil or a non-ionic detergent (Noigen HC) resulted in a 44 to 45-fold increase in extracellular LipL, and the presence of both resulted in a further 56-fold increase. Among the triglycerides tested, triolein was the most effective, with a 50-fold increase in LipL production. A Northern blot hybridization analysis found that the lipL transcript increased in the presence of soybean oil or Noigen HC, indicating that the production of LipL is regulated at the transcriptional level.
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
J
J
Tanaka
T
T
Sudo
F
F
Ihara
T
T
Nihira
Y
Y
Yamada
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA; D003902:Detergents / Q000737:chemistry*; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry; D011549:Pseudomonas / Q000201:enzymology*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013024:Soybean Oil / Q000737:chemistry*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003902:Detergents; D013024:Soybean Oil; D004247:DNA; D008049:Lipase; C120013:lactonizing lipase protein, Pseudomonas; D015719:Single-Strand Specific DNA and RNA Endonucleases