Interplay of C1 and C2 domains of protein kinase C-alpha in its membrane binding and activation.
The regulatory domain of conventional protein kinase C (PKC) contains two membrane-targeting modules, the C2 domain that is responsible for Ca2+-dependent membrane binding of protein, and the C1 domain composed of two cysteine-rich zinc fingers (C1a and C1b) that bind diacylglycerols and phorbol esters. To understand the individual roles and the interplay of the C1 and C2 domains in the membrane binding and activation of PKC, we functionally expressed isolated C1 and C2 domains of PKC-alpha and measured their vesicle binding and monolayer penetration. Results indicate that the C2 domain of PKC-alpha is responsible for the initial Ca2+- and phosphatidylserine-dependent electrostatic membrane binding of PKC-alpha, whereas the C1 domain is involved in subsequent membrane penetration and diacylglycerol binding, which eventually lead to enzyme activation. To determine the roles of individual zinc fingers in the C1 domain, we also mutated hydrophobic residues in the C1a (Trp58 and Phe60) and C1b (Tyr123 and Leu125) domains of the native PKC-alpha molecule and measured the effects of mutations on vesicle binding, enzyme activity and monolayer penetration. Results show that the hydrophobic residues in the C1a domain are essential for the membrane penetration and activation of PKC-alpha, whereas those in the C1b domain are not directly involved in these processes. Based on these results in conjunction with our previous structure-function studies of the C2 domain (Medkova, M., and Cho, W. (1998) J. Biol. Chem. 273, 17544-17552), we propose a mechanism for the in vitro membrane binding and activation of conventional PKC that accounts for the temporal and spatial sequences of PKC activation.
Department of Chemistry, University of Illinois, Chicago, Illinois 60607-7061, USA.
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Medkova
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D000595:Amino Acid Sequence; D002118:Calcium / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010446:Peptide Fragments / Q000235:genetics; D010703:Phorbol Esters; D010718:Phosphatidylserines / Q000494:pharmacology; D011485:Protein Binding; D011493:Protein Kinase C / Q000737:chemistry* / Q000235:genetics; D051571:Protein Kinase C-alpha; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D016335:Zinc Fingers
D004075:Diglycerides; D007527:Isoenzymes; D008081:Liposomes; D008563:Membrane Lipids; D008565:Membrane Proteins; D010446:Peptide Fragments; D010703:Phorbol Esters; D010718:Phosphatidylserines; D011994:Recombinant Proteins; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D002118:Calcium