Biochemical and immunologic comparison of virus-like particles for a rotavirus subunit vaccine.
A parenterally administered rotavirus vaccine composed of virus-like particles (VLPs) is being evaluated for human use. VLPs composed of bovine VP6 and simian VP7 (SA11, G3) proteins (6/7-VLPs) or of bovine VP2, bovine VP6, and simian VP7 (SA11, G3) proteins (2/6/7-VLPs) were synthesized and purified from Sf9 insect cells co-infected with recombinant baculoviruses. 6/7- and 2/6/7-VLP administered parenterally (i.m.) in mice had comparable immunogenicity, but the 2/6/7-VLPs were more homogeneous and stable. The inclusion of the VP2 capsid contributed to particle formation and stability. The adjuvant QS-21 significantly enhanced the immunogenicity of 2/6/7-VLPs over A10H or saline alone. Equivalent serum neutralizing antibody responses were induced over the range of 1-15 microg/dose of 2/6/7-VLPs administered with the range of 5-20 microg/dose of QS-21. The immunogenicity of 2/6/7-VLPs and inactivated SA11 virus were comparable. 2/6/7-VLPs are a promising candidate for a parenterally delivered rotavirus subunit vaccine.
Madore
H P
HP
Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, NY 14586, USA.
Estes
M K
MK
Zarley
C D
CD
Hu
B
B
Parsons
S
S
Digravio
D
D
Greiner
S
S
Smith
R
R
Jiang
B
B
Corsaro
B
B
Barniak
V
V
Crawford
S
S
Conner
M E
ME
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D036022:Capsid Proteins; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D007700:Kinetics; D051379:Mice; D009500:Neutralization Tests; D012401:Rotavirus / Q000737:chemistry* / Q000276:immunology*; D018411:Spodoptera; D014765:Viral Vaccines / Q000737:chemistry / Q000276:immunology; D014771:Virion / Q000737:chemistry* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D014765:Viral Vaccines