Effect of oleanolic acid-cyclodextrin inclusion compounds on dental caries by in vitro experiment and rat-caries model.
Earlier work in vitro showed that oleanolic acid (OA) was a potential inhibitor of insoluble glucan (ISG) synthesis from mutans streptococci (MS). In this study, two oleanolic acid-cyclodextrin inclusion compounds (OA-CDs), oleanolic acid-G1-beta-cyclodextrin (OA-G1-beta CD) and oleanolic acid-beta-cyclodextrin (OA-beta CD), were assayed for their effects on ISG synthesis from Streptococcus mutans MT8148R, and on the growth of oral bacteria. OA-beta CD inhibited ISG synthesis by 55.3 and 37.4% at 62.5 and 15.6 micrograms/ml of OA, respectively. Both OA-CDs inhibited the growth of MS, S. sanguis, and S. salivarius at 4 to 8 micrograms/ml of OA. The anticariogenic effect of the OA-beta CD was examined in a rat-caries model. Rats in the infected control groups showed the highest caries score. The infected treatment group B (0.5% OA in diet) showed lower scores than the control group. These results suggest that OA-beta CD is a potential anti-caries agent.
Department of Paediatric Dentistry, Hiroshima University, School of Dentistry, Japan.
K
K
Kozai
J
J
Suzuki
M
M
Okada
N
N
Nagasaka
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003505:Cyclodextrins / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology*; D004195:Disease Models, Animal; D005936:Glucans / Q000096:biosynthesis; D008297:Male; D009828:Oleanolic Acid / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D016986:Streptococcus sobrinus / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D003505:Cyclodextrins; D005936:Glucans; D009828:Oleanolic Acid