Glass ring-wall sealing to prevent leakage from electrode culture chambers: a technical note.
Entellan is a good sealing substance for culture chamber walls and does not interfere with the growth of the cultured DRG cells. If the Entellan becomes brittle the glass ring can easily be loosened by placing the chamber with the sealed ring for 24 hrs in xylene. Repeated rinsing with xylene and subsequently with hydrated steps of ethanol (100%-70%-30%) allows the ring-wall and chamber floor with its electrodes to be reused.
Dept. Physiology, LUMC, The Netherlands. Marani@physiology.medfac.leidenuniv.nl
E
E
Marani
M
M
Deenen
M H
MH
Goedbloed
W L
WL
Rutten
D000818:Animals; D002478:Cells, Cultured; D015194:Diffusion Chambers, Culture / Q000295:instrumentation*; D005727:Ganglia, Spinal / Q000166:cytology; D005898:Glass; D008839:Microelectrodes; D051381:Rats; D012117:Resins, Synthetic
D012117:Resins, Synthetic; C052885:entellan