Intranasal murine model of Bordetella pertussis infection. I. Prediction of protection in human infants by acellular vaccines.
Bicomponent, tricomponent and pertactin DTPa vaccines were tested in sublethal aerosol, and lethal and sublethal intranasal murine Bordetella pertussis respiratory challenge models. Pertactin and bicomponent vaccines induced protective immunity against lethality but with little or no bacterial clearance. Intranasal challenge discriminated in a reproducible, statistically significant manner between the efficacies of bicomponent and tricomponent DTPa, in agreement with clinical trial data. This discrimination was not observed in the aerosol challenge. Pertactin had a synergistic effect with bicomponent DTPa. Intranasal challenge may be useful as part of the preclinical evaluation of new acellular pertussis formulations or DTPa-based combinations.
Laboratoire des Bordetella, Institut Pasteur, Paris, France. nguiso@pasteur.fr
N
N
Guiso
C
C
Capiau
G
G
Carletti
J
J
Poolman
P
P
Hauser
D000280:Administration, Inhalation; D000281:Administration, Intranasal; D000336:Aerosols; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology*; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D051379:Mice; D008807:Mice, Inbred BALB C; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D017778:Vaccines, Combined / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control*
D000336:Aerosols; D001428:Bacterial Vaccines; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D017778:Vaccines, Combined