[Evaluation of the correlation between the level of IgG antibodies against mycobacterial A60-antigen and tuberculin reactivity in persons without a history of tuberculosis and in active pulmonary tuberculosis patients].
A measurement of antimycobacterial antibodies levels provides important informations about infection with tubercle bacilli and the development of tuberculosis. A tuberculin test as a manifestation of cellular immunity, has lost in diagnostic value. The aim of the present study was to assess the correlation between the level of IgG antibodies against A60 antigen of Mycobacterium bovis BCG and tuberculin reactivity. 213 persons were involved into the study: 104 subjects without history of tuberculosis in the past (Group I) and 109 active pulmonary tuberculosis patients (Group II)--56 culture positive patients and 53 culture negative patients. In all subjects the A60-ELISA test with the use of Immune kit was performed. All subjects were also tuberculin tested by the Mantoux technique using 2 units of tuberculin Rt23. We found that the levels of anti-60 antibodies in patients with active pulmonary tuberculosis were significantly higher than in controls (median value 43 U/ml and 237.5 U/ml respectively; p = 0.0001). We observed 47.1% and 74.3% tuberculin positive subjects in Group I and Group II respectively. We did not find relationship between the level of IgG anti-A60 antibodies and tuberculin reactivity in tuberculin-positive and tuberculin-negative subjects both in controls and active pulmonary tuberculosis patients. We conclude: 1. There is no correlation between A60-ELISA results and tuberculin reaction status. 2. The serological test can better than tuberculin test differentiate subjects with non-active tuberculous infection from active disease.
Katedra i Klinika Chorób Płuc i Gruźlicy AM w Gdańsku.
A
A
Siemińska
L
L
Wolska-Goszka
J M
JM
Słomiński
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012698:Serologic Tests; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology*
D000942:Antigens, Bacterial; D007074:Immunoglobulin G; C055539:mycobacterial A60 antigen
Ocena zalezności pomiedzy stezeniem przeciwciał IgG przeciw antygenowi A60 mykobakterii a odczynem tuberkulinowym u osób bez wywiadu chorobowego w kierunku gruźlicy oraz chorych na czynna gruźlice płuc.