Random amplified polymorphic DNA analysis of Ustilago violacea.
Isolates of the two mating type strains of the basidiomycete phytopathogen Ustilago violacea (Pers.) Roussel [a.k.a Microbotryum violaceum (Pers.:Pers.) Deml and Oberw] are restricted in their host range to one or a few species of Caryophyllaceae (Pinks). Molecular genetics maps in this species are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross and more recently through electrophoretic karyotypes and chromosomal polymorphism using CHEF gel analysis. However, currently, polymorphisms in genomic fingerprints generated by arbitrarily primed polymerase chain reaction (PCR) can distinguish between strains of almost any organism, which is useful in genetic mapping. The objective of this project was to use PCR technology, 40 Operon 10-mer primers, and 5 simple sequence repeat (SSR) primers, designed on microsatellite sequences to determine their efficiency in detecting intraspecific differences between the genomic DNA of the two mating type strains of U. violacea (a1 and a2). Polymorphism in the banding patterns of the DNA samples was detected after PCR and electrophoresis in 1.4% agarose gels. This polymorphic intraspecific variation will be utilized to detect cryptic and trans-active transposable elements in U. violacea.
Chicago State University, Department of Biological Sciences, Illinois 60628, USA. C-Henry@csu.edu
C E
CE
Henry
P
P
Okoro
E
E
Steward-Clark
E D
ED
Garber
M
M
Ruddat
D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D009876:Operon; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014588:Ustilago / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial