Substance P immunoreactive nerves and interstitial cells of Cajal in the rat and guinea-pig ileum. A histochemical and quantitative study.
The substance P (SP)-containing nerves at the deep (DMP) and myenteric (MP) plexuses and the related interstitial cells of Cajal (ICC-DMP and ICC-MP) were immunohistochemically studied in rat and guinea-pig ileum. All the ICC expressed SP-preferred receptor NK1r: the ICC-DMP showed an intense and the ICC-MP a faint NK1r-immunoreactivity(IR). c-kit-labeling confirmed that they were ICC. The SP-IR nerves at the DMP were significantly more numerous in the guinea-pig than in the rat, and more numerous than those at the MP in both animal species. All the ICC-DMP in the guinea-pig and half of them in the rat were close to SP-IR nerves. The ICC-MP were rarely near to SP-IR nerves in either species. The SP-innervation shows interspecies differences at the DMP that imply a different tachykinergic control of the local ICC.
Department of Anatomy, Histology and Forensic Medicine, University of Florence, Italy.
M G
MG
Vannucchi
L
L
Corsani
M S
MS
Faussone-Pellegrini
D000818:Animals; D019084:Fluorescent Antibody Technique, Indirect; D006168:Guinea Pigs; D007082:Ileum / Q000737:chemistry / Q000294:innervation*; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000737:chemistry; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D013373:Substance P / Q000032:analysis* / Q000737:chemistry
D013373:Substance P