Purification and properties of proline-rich antimicrobial peptides from sheep and goat leukocytes.
We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5alpha and ChBac5, to detailed analysis. OaBac5alpha and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. While the peptides remained active against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Listeria monocytogenes in 100 mM NaCl, they lost activity against Staphylococcus aureus and Candida albicans under these conditions. ChBac5 was shown to bind lipopolysaccharide, a property that could enhance its ability to kill gram-negative bacteria. Proline-rich Bac5 peptides are highly conserved in ruminants and may contribute significantly to their innate host defense mechanisms.
Shamova
O
O
Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA.
Brogden
K A
KA
Zhao
C
C
Nguyen
T
T
Kokryakov
V N
VN
Lehrer
R I
RI
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D003001:Cloning, Molecular; D047091:Eosinophil Granule Proteins; D006041:Goats / Q000097:blood*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D007962:Leukocytes / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D012756:Sheep / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; C078861:CATHL2 protein, Bos taurus; D047091:Eosinophil Granule Proteins; D008070:Lipopolysaccharides; D010455:Peptides; D013755:Tetradecanoylphorbol Acetate