Extraction and high-performance liquid chromatographic separation of selected pyrene and benzo[a]pyrene sulfates and glucuronides: preliminary application to the analysis of smokers' urine.
In the study of the complex mixture of urinary metabolites derived from polycyclic aromatic hydrocarbon compounds, it is desirable to simplify the analysis through separation of classes of compounds. We have developed a liquid chromatography (LC) method for the separation of selected sulfate and glucuronide conjugate isomers derived from hydroxybenzo[a]pyrenes (OH-BaP) and hydroxypyrenes. This LC method was utilized in the preliminary analysis of the urine of smokers by combining it with an extraction technique employing tetra-n-butyl-ammonium ion as a coupling agent to generate a 1:1 complex, extractable in chloroform at low pH prior to LC analysis.
Johnson
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Department of Physical Sciences and Computer Science, Medgar Evers College, The City University of New York, Brooklyn 11225, USA.
Greenberg
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D001564:Benzo(a)pyrene / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005965:Glucuronates / Q000652:urine; D006801:Humans; D011721:Pyrenes / Q000378:metabolism*; D015203:Reproducibility of Results; D012907:Smoking / Q000652:urine*; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids / Q000652:urine
D005965:Glucuronates; D011721:Pyrenes; D013464:Sulfuric Acids; D001564:Benzo(a)pyrene; C030984:pyrene