Involvement of MCP-1 and M-CSF in glomerular foam cell formation in ExHC rats.
BACKGROUND
An increase in glomerular macrophages (MO) is considered a potential effector mechanism by which hypercholesterolemia exacerbates glomerular injury. To investigate the mechanism underlying recruitment of MO into glomeruli, the expression of glomerular monocyte chemoattractant protein-1 (MCP-1) and macrophage colony-stimulating factor (M-CSF) mRNA were examined using a lipid-induced glomerular injury rat model.


METHODS
Eight-week-old male ExHC rats, a strain susceptible to hyperlipidemia, were divided into the following 4 groups: a control group (C), a high cholesterol diet group (HH), a high cholesterol diet/standard diet group (HN), which were fed a high cholesterol diet for the first 4 weeks and a standard diet for the following 4 weeks, and a probucol-treatment group (PT). Both MCP-1 and M-CSF mRNA expression in glomeruli were analyzed using the RT-PCR method. An additional experimental group (M) fed a high cholesterol diet was administered M-CSF daily for 4 weeks.


RESULTS
The expression of MCP-1 mRNA in glomeruli increased accompanied by an increased total serum cholesterol level in HH and HN. However, M-CSF mRNA expression was significantly suppressed at 1 or 2 weeks and gradually increased to almost basal levels. In the PT group, MCP-1 mRNA expression was suppressed. The early suppression of M-CSF mRNA expression was inhibited in PT. Renal histology showed a significant increase in foam cells in glomeruli in HH and HN rats at 4 weeks. HH rats showed increased and expanded foam cells at 8 weeks. In HN rats, however, foam cells decreased significantly after the transfer to a standard diet from a high cholesterol diet. The MCP-1 mRNA expression was suppressed after the transfer. In the PT group, foam cell formation was also suppressed. Foam cells were identified as MO. M-CSF-treatment significantly suppressed foam cell formation in glomeruli when compared with the untreated group levels.


CONCLUSIONS
These findings suggest that hypercholesterolemia stimulated the expression of MCP-1 in glomeruli and attracted the MO into glomeruli. They also suggest that the reduction of hypercholesterolemia after the change in diet or treatment with probucol suppressed glomerular injury by suppressing the glomerular MCP-1 expression. M-CSF may suppress the recruitment of MO into glomeruli and foam cell formation at an early stage of hypercholesterolemia-induced glomerular injury.
Kodama
N
N
Third Department of Internal Medicine, Wakayama Medical College, Japan.
Otani
H
H
Yamada
Y
Y
Mune
M
M
Yukawa
S
S
D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D018932:Chemokine CCL2 / Q000235:genetics*; D002791:Cholesterol, Dietary / Q000009:adverse effects; D005487:Foam Cells / Q000187:drug effects / Q000473:pathology*; D006937:Hypercholesterolemia / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology*; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000494:pharmacology; D008297:Male; D011341:Probucol / Q000494:pharmacology; D011507:Proteinuria / Q000517:prevention & control / Q000652:urine; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats
D000924:Anticholesteremic Agents; D018932:Chemokine CCL2; D002791:Cholesterol, Dietary; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D011341:Probucol