Molecular cloning of a cDNA encoding 14-3-3 protein from the protozoan Tetrahymena pyriformis and its mRNA expression during synchronous division.
The 14-3-3 proteins have been identified in a wide variety of eukaryotic cells and diverse biochemical properties have been ascribed to them. Here we have cloned a cDNA encoding 14-3-3 protein from a cDNA library of Tetrahymena pyriformis. This cDNA (Tp14-3-3) encoded an open reading frame consisting of 244 amino acids with predicated molecular mass as 28.1kDa. The predicted protein shares 59.2%, 56.5% and 59.2% identity to Entamoeba histolytica 14-3-3-1, Schizosaccharomyces pombe Rad 24 and human 14-3-3 zeta/delta respectively. On the basis of comparison with other 14-3-3 proteins, two of the putative functional domains (dimerization domain and annexin-similarity domain) were found in Tp14-3-3. In order to know the role of Tp14-3-3 in Tetrahymena, its mRNA levels during synchronous division were examined by Northern blot analysis. There was a marked increase in Tp14-3-3 mRNA level at 45min after the end of heat treatment, followed by a gradual decrease. These results suggest that the Tp14-3-3 mRNA level might vary during the cell cycle. The accumulation of Tp14-3-3 mRNA before cell division was assumed to be a prerequisite for the initiation of synchronous cell division.
Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500-8705, Japan.
Y
Y
Zhao
S
S
Nakashima
Y
Y
Nozawa
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013769:Tetrahymena pyriformis / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D014446:Tyrosine 3-Monooxygenase