JNK activation is not required for Fas-mediated apoptosis.
Fas ligation in the presence of cycloheximide induced Jun N-terminal kinase 1 (JNK1) and JNK2 phosphorylation, caspase activation and cell death in the IL-3-dependent cell line BAF3. Fas-mediated apoptosis was prevented by expression of dominant negative FADD but not inhibited by IL-3. To investigate the role of JNK activation in this process, we examined cells over-expressing a JNK-specific phosphatase M3/6. M3/6 prevented Fas stimulation of JNK, but did not affect Fas-mediated caspase activation or cell death, demonstrating that JNK activation is not required for these processes.
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Department of Immunology, University College London, UK.
Smith
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Ashworth
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Collins
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D029681:Arabidopsis Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000502:physiology*; D020169:Caspases / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation; D053222:Fas Ligand Protein; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000473:pathology; D007377:Interleukin-3 / Q000494:pharmacology; D048031:JNK Mitogen-Activated Protein Kinases; D008562:Membrane Glycoproteins / Q000502:physiology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D019014:fas Receptor / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D029681:Arabidopsis Proteins; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D011500:Protein Synthesis Inhibitors; D019014:fas Receptor; D003513:Cycloheximide; D044943:Fatty Acid Desaturases; C084083:Fad7 protein, Arabidopsis; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D020169:Caspases