Visualization of in vitro protein-rejecting properties of PEGylated stealth polycyanoacrylate nanoparticles.
The in vitro protein-rejecting properties of PEG-coated polyalkylcyanoacrylate (PACA) nanoparticles were for the first time visualized after freeze-fracture of the nanoparticles pre-incubated with fibrinogen as a model blood protein. The reduced protein association to the nanoparticles was evidenced also by two-dimensional PAGE after incubation of the nanoparticles with human plasma. In vivo experiments showed the 'stealth' long-circulating properties of the PEGylated nanoparticles after intravenous administration to mice. Thus, the images obtained after nanoparticle-protein incubation were predictive of the behavior observed in vivo. In conclusion, freeze-fracture analysis represents a novel and original qualitative approach to investigate the interactions between proteins and particulate systems.
Université Paris-Sud XI, URA CNRS 1218-Physico-Chimie, Pharmacotechnie, Biopharmacie, Châtenay-Malabry, France.
M T
MT
Peracchia
S
S
Harnisch
H
H
Pinto-Alphandary
A
A
Gulik
J C
JC
Dedieu
D
D
Desmaële
J
J
d'Angelo
R H
RH
Müller
P
P
Couvreur
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003487:Cyanoacrylates / Q000737:chemistry*; D004337:Drug Carriers; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005340:Fibrinogen / Q000737:chemistry*; D005614:Freeze Fracturing; D006801:Humans; D007275:Injections, Intravenous; D051379:Mice; D008815:Mice, Inbred Strains; D011092:Polyethylene Glycols / Q000737:chemistry*
D001672:Biocompatible Materials; D001798:Blood Proteins; D003487:Cyanoacrylates; D004337:Drug Carriers; C121140:polyethyleneglycol polyhexadecylylcyanoacrylate copolymer; D011092:Polyethylene Glycols; D005340:Fibrinogen