Determination of 5'-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence of highly conserved sequence motifs.
We determined the untranslated 5'-leader sequence for three different isolates of porcine reproductive and respiratory syndrome virus (PRRSV): pathogenic European- and American-types, as well as an American-type vaccine strain. 5'-leader from European- and American-type PRRSV differed in length (220 and 190 nt, respectively), and exhibited only approximately 50% nucleotide homology. Nevertheless, highly conserved areas were identified in the leader of all 3 PRRSV isolates, which constitute candidate motifs for binding of protein(s) involved in viral replication. These comparative data provide a priori knowledge for mutational identification of virulence determinants in the 5' nontranslated part of the PRRSV genome.
Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
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MB
Oleksiewicz
A
A
Bøtner
J
J
Nielsen
T
T
Storgaard
D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D005060:Europe; D008969:Molecular Sequence Data; D009656:North America; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014779:Virus Replication
D020121:5' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral