Tannic acid and thiocarbohydrazide as structural reinforcement agents in the preparation of rabbit knee articular cartilage for the scanning electron microscope.
Samples from seven sectors of the rabbit knee articular cartilage were shaved and prepared for the scanning electron microscope using either tannic acid, thiocarbohydrazide or nothing (control). Surface morphology was found to be more typical to a given sector and less so to a specific preparation procedure. Rough areas were recorded from load-bearing sectors, while smooth areas appeared on load-free ones. However, fibrillations were discerned on control load-bearing sectors only, and pits and humps were never detected. Tannic acid and thiocarbohydrazide may have exerted their structural reinforcing effect on the tissue preservation by enhancing the binding of osmium tetroxide to it, possibly along with that of other soluble tissue constituents.
Unit of Electron Microscopy, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
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D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000648:ultrastructure; D005269:Femur / Q000187:drug effects / Q000648:ultrastructure; D005404:Fixatives / Q000494:pharmacology*; D006834:Hydrazines / Q000494:pharmacology*; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D007719:Knee Joint / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013977:Tibia / Q000187:drug effects / Q000648:ultrastructure
D005404:Fixatives; D006834:Hydrazines; D047348:Hydrolyzable Tannins; C011368:thiocarbohydrazide