Interaction of human immunodeficiency virus-derived vectors with wild-type virus in transduced cells.
The interaction of human immunodeficiency virus (HIV)-derived vectors with wild-type virus was analyzed in transduced cells. Vector transcripts upregulated by infection had no measurable effect on HIV type 1 (HIV-1) expression but competed efficiently for encapsidation, inhibiting the infectivity and spread of HIV-1 in culture and leading to mobilization and recombination of the vector. These effects were abrogated with a self-inactivating vector.
Bukovsky
A A
AA
Cell Genesys, Foster City, California 94404, USA.
Song
J P
JP
Naldini
L
L
D002472:Cell Transformation, Viral; D005822:Genetic Vectors; D016679:Genome, Viral; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D019065:Virus Assembly; D014779:Virus Replication