The distribution and gene expression of adrenomedullin in the rat brain: peptide/mRNA and precursor/active peptide relationships.
The highest immunoreactive adrenomedullin (AM) concentrations were found in the pituitary gland, hypothalamus and brainstem. Rat preproadrenomedullin mRNA levels were highest in the pituitary, followed by the hypothalamus. The peptide to mRNA ratios are higher in the neurointermediate lobe, brainstem and hypothalamus. Immunocytochemical study showed discrete localization of AM immunostaining in various brain regions including the cerebral cortex, hypothalamus and brainstem and in the pituitary gland. By gel filtration chromatography, the precursor peaks were low in the pituitary, hypothalamus and brainstem but high in other brain regions.
Department of Physiology, Faculty of Medicine, The University of Hong Kong, People's Republic of China.
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D053607:Adrenomedullin; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000096:biosynthesis / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D010902:Pituitary Gland / Q000737:chemistry; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
D009479:Neuropeptides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C112034:preproadrenomedullin; D053607:Adrenomedullin