Myeloid cell-lineage and premylocytic-stage-specific- expression of themouse myeloperoxidase gene is controlled at initiation as well as elongation levels of transcription.
The myeloperoxidase (MPO) is an important microbicidal protein present at high concentration in the primary granule of mature granulocyte and its expression is regulated in both myeloidcell-lineage and premyelocytic-stage-specific manners. A better understanding of the underlying control mechanisms should provide insights into the temporal and co-ordinate regulation of the gene expression during granulopoiesis. We have identified its promoter by mapping the start(s) of transcription using various molecular approaches together with demonstrating the promoter function of the relevant DNA segment in a transient transfection reporter assay. Besides the major start of transcription mapped at G residue, 11 nucleotide upstream of the 3' end of exon 0, the usage of that is specific to the MPO expressing cell lines, we have shown that irrespective of the MPO-expression status of the hematopoietic cells, transcription occurs broadly within a two kb region upstream of the 5' proximity of the gene, and is largely terminated in intron 2. These data support a model of the premyelocytic-stage-specific MPO expression, the control of which is operated at initiation as well as elongation levels of transcription.
Department of Medical Oncology, University of Glasgow, UK. gpma66@udcf.gla.ac.uk
J D
JD
Zhu
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D019070:Cell Lineage / Q000235:genetics; D018387:Codon, Initiator; D004742:Enhancer Elements, Genetic; D005091:Exons; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010441:Peptide Chain Elongation, Translational / Q000235:genetics; D010442:Peptide Chain Initiation, Translational / Q000235:genetics; D009195:Peroxidase / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells / Q000201:enzymology*; D014158:Transcription, Genetic
D018387:Codon, Initiator; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D009195:Peroxidase