Characterization of Methanosarcina mazei N2M9705 isolated from an aquaculture fishpond.
A new methanogenic isolate, designated as strain N2M9705 (=OCM 668), was isolated from an aquaculture fishpond near Wang-gong, Taiwan. This strain grew on trimethylamine and methanol, but it did not catabolize H2-CO2, acetate, or formate. The cells were stained Gram-negative, nonmotile, irregular coccus 0.6-0.8 micrometer in diameter. Gas vacuoles were observed and cell aggregated to form various sizes of granules. Cells grew optimally at 32 degrees -37 degrees C with 1% NaCl. The pH range of growth was 6.2-7.4, and higher pH inhibited the cell growth. The cells grew well in minimal medium, but growth was greatly stimulated by yeast extract and peptone. A comparison of 16S rDNA sequences of this organism phylogenetically related to Methanosarcina mazei. This is the first report of methyltrophic methanogenic isolated from an aquaculture fishpond.
Lai
M C
MC
Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C.
Shu
C M
CM
Chiou
M S
MS
Hong
T Y
TY
Chuang
M J
MJ
Hua
J J
JJ
D000818:Animals; D017756:Aquaculture; D003470:Culture Media; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005399:Fishes / Q000502:physiology; D017020:Methanosarcina / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D003470:Culture Media; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S