A putative host cell receptor for tick-borne encephalitis virus identified by anti-idiotypic antibodies and virus affinoblotting.
Anti-idotypic monoclonal antibodies (anti-ID MAbs) were made against two mouse MAbs that neutralize the infectivity of the tick-borne encephalitis (TBE) virus. Three of the anti-ID MAbs (1) inhibited the binding of respective idiotypic MAb to the TBE virus antigen, (2) inhibited the infectivity of TBE virus when preincubated with virus-susceptible cells, and (3) bound to the surface of virus-susceptible but not virus-nonsusceptible cells. They recognized a 35-kD protein in immunoblotting analysis. Identification of this protein as a component of a putative TBE virus receptor was supported by the viroblot technique. In this assay, two polypeptide signals of 35 and 18 kD were obtained after incubation of blotted cell membrane proteins with the TBE virion antigen.
Kopecký
J
J
Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, Ceské Budejovice, Czech Republic. jan@paru.cas.cz
Grubhoffer
L
L
Kovár
V
V
Jindrák
L
L
Vokurková
D
D
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000956:Antigens, Viral / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line / Q000378:metabolism; D002478:Cells, Cultured; D004669:Encephalitis Viruses, Tick-Borne / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D011991:Receptors, Virus / Q000737:chemistry*; D013047:Specific Pathogen-Free Organisms
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011991:Receptors, Virus