A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation.
The bacterial metabolism of short-chain aliphatic alkenes occurs via oxidation to epoxyalkanes followed by carboxylation to beta-ketoacids. Epoxyalkane carboxylation requires four enzymes (components I-IV), NADPH, NAD(+), and a previously unidentified nucleophilic thiol. In the present work, coenzyme M (2-mercaptoethanesulfonic acid), a compound previously found only in the methanogenic Archaea where it serves as a methyl group carrier and activator, has been identified as the thiol and central cofactor of aliphatic epoxide carboxylation in the Gram-negative bacterium Xanthobacter strain Py2. Component I catalyzed the addition of coenzyme M to epoxypropane to form a beta-hydroxythioether, 2-(2-hydroxypropylthio)ethanesulfonate. Components III and IV catalyzed the NAD(+)-dependent stereoselective dehydrogenation of R- and S-enantiomers of 2-(2-hydroxypropylthio)ethanesulfonate to form 2-(2-ketopropylthio)ethanesulfonate. Component II catalyzed the NADPH-dependent cleavage and carboxylation of the beta-ketothioether to form acetoacetate and coenzyme M. These findings evince a newfound versatility for coenzyme M as a carrier and activator of alkyl groups longer in chain-length than methane, a function for coenzyme M in a catabolic pathway of hydrocarbon oxidation, and the presence of coenzyme M in the bacterial domain of the phylogenetic tree. These results serve to unify bacterial and Archaeal metabolism further and showcase diverse biological functions for an elegantly simple organic molecule.
Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322-0300, USA.
J R
JR
Allen
D D
DD
Clark
J G
JG
Krum
S A
SA
Ensign
D000475:Alkenes / Q000378:metabolism; D001105:Archaea / Q000201:enzymology; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000378:metabolism*; D006838:Hydrocarbons / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015080:Mesna / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011407:Propane / Q000031:analogs & derivatives; D013237:Stereoisomerism; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000475:Alkenes; D002247:Carbon Isotopes; D004852:Epoxy Compounds; D006838:Hydrocarbons; D013438:Sulfhydryl Compounds; D009243:NAD; D009249:NADP; D008780:Methyltransferases; D002262:Carboxy-Lyases; C109739:epoxide carboxylase; D015080:Mesna; D011407:Propane