Effect of some phthalate esters in human cells in the comet assay.
Phthalate esters are among the most extensively used industrial chemicals and are widely distributed in the environment. Di-(2-ethylhexyl)phthalate (DEHP) and its hydrolysis product mono-(2-ethylhexyl)phthalate (MEHP) have been examined for genotoxic activity on previous occasions. Only MEHP was found to cause chromosome damage in CHO cells but was without effect in the sister chromatid exchange and hypoxanthine guanine phosphoribosyl assay. DEHP was found to be a weak direct acting mutagen in Salmonella typhimurium strain TA100, the mutagenic activity of which could be abolished by rat liver microsomes (S9 mix). The clastogenicity and weak mutagenicity suggest a possible contributory role for these compounds in the observed carcinogenicity of the phthalates, which have been thought predominantly to be linked to cancer pathology through proliferation of hepatic peroxisomes. The present study showed that these compounds could produce DNA damage in human blood cells in the Comet assay and also, that rat liver microsomes could abolish the effect of DEHP. Thus in the intact animal, no response may be observed.
Anderson
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BIBRA International, Carshalton, Surrey, United Kingdom.
Yu
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TW
Hinçal
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D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004249:DNA Damage; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology*; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009153:Mutagens; D004051:Diethylhexyl Phthalate; D007041:Hypoxanthine Phosphoribosyltransferase; C016599:mono-(2-ethylhexyl)phthalate