Regions of the pea Lhcb1*4 promoter necessary for blue-light regulation in transgenic Arabidopsis.
Pea (Pisum sativum) and Arabidopsis contain similar, if not identical, blue-light (BL)-responsive systems that alter expression of specific members of the Lhcb (light-harvesting chlorophyll-binding) gene family. In both plants a single, short pulse of low-fluence BL (threshold = 10(-1) micromol m-2) causes an increase in the rate of transcription from specific members of the Lhcb gene family in etiolated seedlings. Constructs of the BL-regulated pea Lhcb1*4 promoter (PsLhcb1*4) were created, which altered sequences previously implicated in light responses, deleted the 5'-promoter sequence, or removed the 5'-untranslated region. These constructs were tested for BL induction in transgenic Arabidopsis. The PsLhcb1*4 promoter deletions to -150 bp maintained normal fluence response, time course, and reciprocity characteristics. The 5'- untranslated region contained enhancer elements, but was not necessary for BL induction. The -95 to +2 promoter was capable of responding to BL, whereas sequences from -50 were not. Promoters that lack conserved light-regulatory elements or sequences directly implicated in phytochrome and circadian responses retained BL activity, suggesting that the low-fluence BL response utilizes regions of the promoter independent of those that modulate the phytochrome and circadian responses.
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
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Folta
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Kaufman
D020121:5' Untranslated Regions; D017360:Arabidopsis / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D001483:Base Sequence; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D018532:Pisum sativum / Q000235:genetics* / Q000528:radiation effects; D010750:Phosphoproteins / Q000235:genetics; D010834:Phytochrome / Q000096:biosynthesis; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D020121:5' Untranslated Regions; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010750:Phosphoproteins; D010834:Phytochrome; C103646:NPH1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases