Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection.
We describe the separation of an active glutamate dehydrogenase [GDH (NADP+)] enzyme from the plasma of patients with P. falciparum infection using columns of sepharose anti-GDH (NADP+) of Proteus spp. The activity of this enzyme was also detected in P. falciparum culture supernatant. The parasitic origin of this enzyme was suggested by western blot analysis using anti-P. falciparum culture supernatant and anti-whole parasite antibodies. The differential inhibition of the P. falciparum GDH (NADP+) indicates that some epitopes recognised by the antibodies in both preparations may be different. The determination of P. falciparum GDH (NADP+) activity could be developed into a specific technique for the diagnosis of falciparum malaria.
Universidad Central de Venezuela, Secciôn de Inmunoquímica, Caracas, Venezuela.
A
A
Rodriguez-Acosta
N
N
de Dominguez
I
I
Aguilar
M E
ME
Girón
D000818:Animals; D005969:Glutamate Dehydrogenase / Q000097:blood* / Q000378:metabolism; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000201:enzymology*; D010963:Plasmodium falciparum / Q000201:enzymology
D005969:Glutamate Dehydrogenase