Specific sequence-directed anti-bilitranslocase antibodies as a tool to detect potentially bilirubin-binding proteins in different tissues of the rat.
The hypothesis that the uneven distribution of bilirubin in the organism, which occurs in hyperbilirubinemia, could reflect an uneven distribution of bilirubin-binding proteins was tested by searching for peptides containing the bilirubin-binding motif identified in bilitranslocase (Battiston et al., 1998). In the rat, positive proteins bands were found to be present only in the liver, gastric mucosa and central nervous system. The electrophoretic mobilities of the positive compounds in the liver and stomach were identical to that of purified bilitranslocase (38 kDa). In the brain, on the contrary, two peptides were found with molecular masses of 79 and 34 kDa, respectively. Their distribution pattern in the central nervous system was different for each of them.
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Italy.
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L
Battiston
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A
Macagno
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S
Passamonti
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F
Micali
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GL
Sottocasa
D000818:Animals; D000918:Antibody Specificity; D001663:Bilirubin / Q000378:metabolism*; D001665:Binding Sites / Q000276:immunology; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology*; D002570:Ceruloplasmin; D005753:Gastric Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology*; D051381:Rats; D014018:Tissue Distribution
D002352:Carrier Proteins; D008565:Membrane Proteins; C488927:bilirubin binding protein, rat; D002570:Ceruloplasmin; C033838:bilitranslocase; D001663:Bilirubin