Influence of diet on the pattern of gastrointestinal biotransformation of netobimin and albendazole sulphoxide in sheep.
The in vitro biotransformation of the anthelmintic compounds, netobimin (NTB) pro-drug and albendazole sulphoxide (ABZSO), by ruminal fluid obtained from sheep fed either hay or concentrate-based diets was investigated. No metabolic activity was observed in boiled samples of ruminal fluid, which confirms the importance of ruminal microflora in the metabolism of the xenobiotics under investigation. NTB pro-drug was efficiently biotransformed by ruminal fluid in vitro. Albendazole (ABZ) and its sulphoxide derivative were the metabolic products recovered. The thioether ABZ was formed by sulphoreduction of ABZSO by ruminal fluid in vitro. A more efficient nitroreduction of NTB and sulphoreduction of ABZSO were observed for ruminal fluid collected from sheep fed the concentrate diet. The type of diet determines the composition and distribution of the microbial population in the rumen; this affects the pattern of drug biotransformation in the gastrointestinal tract, which may impact on drug therapy.
Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro, Tandil, Argentina.
G
G
Virkel
A
A
Lifschitz
A
A
Pis
C
C
Lanusse
D015766:Albendazole / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D000871:Anthelmintics / Q000378:metabolism*; D001562:Benzimidazoles / Q000032:analysis; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004032:Diet / Q000662:veterinary*; D018565:Food-Drug Interactions; D006146:Guanidines / Q000378:metabolism*; D066298:In Vitro Techniques; D011355:Prodrugs / Q000378:metabolism; D015203:Reproducibility of Results; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology; D012756:Sheep / Q000378:metabolism*
D000871:Anthelmintics; D001562:Benzimidazoles; D006146:Guanidines; D011355:Prodrugs; C011321:oxibendazole; D015766:Albendazole; C049626:netobimin