Biological activity and pathological implications of misfolded proteins.
The physiological metabolism of proteins guarantees that different cellular compartments contain the appropriate concentration of proteins to perform their biological functions and, after a variable period of wear and tear, mediates their natural catabolism. The equilibrium between protein synthesis and catabolism ensures an effective turnover, but hereditary or acquired abnormalities of protein structure can provoke a premature loss of biological function, an accelerated catabolism and diseases caused by the loss of an irreplaceable function. In certain proteins, abnormal structure and metabolism are associated with a strong tendency to self-aggregation into a polymeric fibrillar structure, and in these cases the disease is not principally caused by the loss of an irreplaceable function but by the action of this new biological entity. Amyloid fibrils are an apparently inert, insoluble, mainly extracellular protein polymer that kills the cell without tissue necrosis but by activation of the apoptotic mechanism. We analyzed the data reported so far on the structural and functional properties of four prototypic proteins with well-known biological functions (lysozyme, transthyretin, beta 2-microglobulin and apolipoprotein AI) that are able to create amyloid fibrils under certain conditions, with the perspective of evaluating whether the achievement of biological function favors or inhibits the process of fibril formation. Furthermore, studying the biological functions carried out by amyloid fibrils reveals new types of protein-protein interactions in the transmission of messages to cells and may provide new ideas for effective therapeutic strategies.
Dipartimento di Biochimica, Università degli Studi di Pavia, Italy.
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Bellotti
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Mangione
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Stoppini
D000544:Alzheimer Disease / Q000378:metabolism; D019943:Amino Acid Substitution; D000682:Amyloid / Q000737:chemistry; D000686:Amyloidosis / Q000145:classification / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000938:Antigen-Presenting Cells / Q000378:metabolism; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000235:genetics; D001704:Biopolymers / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry; D011228:Prealbumin / Q000737:chemistry / Q000235:genetics; D011487:Protein Conformation; D017510:Protein Folding; D006435:Renal Dialysis / Q000009:adverse effects; D013329:Structure-Activity Relationship; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000235:genetics
D000682:Amyloid; D016632:Apolipoprotein A-I; D001704:Biopolymers; D009419:Nerve Tissue Proteins; D011228:Prealbumin; D001613:beta 2-Microglobulin; D009113:Muramidase