A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses.
A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5' terminus and 148 nts of the 3' terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5' and 3' junction borders of reported potexvirus D RNAs reveal a 65.2-84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.
Yeh
T Y
TY
Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.
Lin
B Y
BY
Chang
Y C
YC
Hsu
Y H
YH
Lin
N S
NS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004279:DNA, Viral; D003673:Defective Viruses / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D017863:Potexvirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral; D011995:Recombination, Genetic; D017423:Sequence Analysis, RNA; D014026:Nicotiana
D004279:DNA, Viral; D012367:RNA, Viral