Effects of the excretory/secretory products of Trichostrongylus colubriformis on the growth of different cell lines.
The effects of the excretory/secretory (ES) products of the parasitic nematode Trichostrongylus colubriformis were examined on the proliferation of seven cell lines derived from a digestive or non-digestive origin. The excretory/secretory products of T. colubriformis were incorporated in the culture medium of the different cell lines and cell proliferation was measured by means of the 5-bromo-2'-deoxy-uridine (Brdu) assay. An increase in cell numbers was found with the three epithelial intestinal cells (RIC, IEC-6, IRD-98) and with epithelial kidney cells (MDCK). In contrast, an inhibition in the proliferation of epithelial ovarian cells (CHO) and fibroblasts (3T3) was observed with the addition of the excretory/secretory products and no effect was detected on the cell growth of hepatocytes (HepG2). These data are discussed with respect to the tissue specificity of the existing mitogenic effect of the worms on the intestinal crypt cells during parasitism.
INRA, Station de Pathologie Aviaire et de Parasitologie, CR de Tours, Nouzilly, France. nbc@clyde.unimelb.edu.au
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D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects; D000818:Animals; D016466:CHO Cells / Q000166:cytology / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000166:cytology / Q000187:drug effects*; D006224:Cricetinae; D004285:Dogs; D005260:Female; D015801:Helminth Proteins / Q000494:pharmacology*; D051379:Mice; D011817:Rabbits; D014254:Trichostrongylus / Q000378:metabolism*
D015801:Helminth Proteins