Expression of hMSH2 correlates with in vitro chemosensitivity to CDDP cytotoxicity in oral and oropharyngeal carcinoma.
We investigated the expression of hMSH2, a human mutS homologue from chromosome 2p, in oral and oropharyngeal squamous cell carcinoma (SCC) by an immunohistochemical technique and performed tumor in vitro chemosensitivity testing. In 58 oral and oropharyngeal SCC, the hMSH2 positive score was inversely associated with tumor size, but not with other clinical parameters. Among five anticancer drugs (cisplatin (CDDP), 5-FU, peplomycin, mitomycin C and doxorubicin), only for CDDP was sensitivity to cytotoxicity correlated with the hMSH2 positive score. The susceptibility of hMSH2-positive tumors to CDDP killing was significantly higher than that of hMSH2-negative tumors. Immunohistochemical results regarding hMSH2 are promising in the evaluation of the sensitivity of cancer cells to CDDP cytotoxicity and enable one to select patients for adjuvant chemotherapy for oral and oropharyngeal SCC.
Fujieda
S
S
Department of Otorhinolaryngology, Fukui Medical University, Yoshida-gun, Japan. sfujieda@fmsrsa.fukui-med.ac.jp
Tanaka
N
N
Sunaga
H
H
Noda
I
I
Sugimoto
C
C
Tsuzuki
H
H
Saito
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000145:classification / Q000188:drug therapy / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D004317:Doxorubicin / Q000494:pharmacology; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology; D009062:Mouth Neoplasms / Q000145:classification / Q000188:drug therapy / Q000378:metabolism*; D051718:MutS Homolog 2 Protein; D009959:Oropharyngeal Neoplasms / Q000145:classification / Q000188:drug therapy / Q000378:metabolism*; D017663:Peplomycin / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects*; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000187:drug effects
D000970:Antineoplastic Agents; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D016685:Mitomycin; D017663:Peplomycin; D004317:Doxorubicin; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein; D002945:Cisplatin; D005472:Fluorouracil