Construction and genetic analysis of S. cerevisiae deletants of six novel ORFs from chromosome II.
We have constructed S. cerevisiae strains carrying genomic deletions of six ORFs from the left arm of chromosome II (YBL018c, YBL019w, YBL024w, YBL042c, YBL043w and YBL046w) in both FY1679 and W303 backgrounds. We have found that YBL018c is an essential gene in yeast, whereas the other five genes are non-essential. We have developed plasmids carrying deletion cassettes that can be used to delete any of the six genes in S. cerevisiae by transforming to G418-resistance, as well as centromeric plasmids containing the cognate genes.
Departamento de Genética, Facultad de Biología, Universidad de Sevilla 41012 Sevilla, Spain.
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Malagón
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Aguilera
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D017931:DNA Primers; D017353:Gene Deletion; D020043:Genes, Essential; D005800:Genes, Fungal; D016366:Open Reading Frames / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers