Distribution and activity of transglutaminase in rat brain carcinogenesis and in gliomas.
Tissue transglutaminase is a calcium-dependent enzyme which may influence cell morphology, cytoskeletal processes and membrane functions. During rat brain carcinogenesis induced by transplacental administration of N-ethyl-N-nitrosourea to BD IX rats, cytosolic tissue transglutaminase activity was increased by about 140% at 30 days of extrauterine life and returned towards the control values at 3-5 months. In the particulate fraction, enzyme activity progressively increased, reaching values similar to those present in the developed gliomas. Tissue transglutaminase activity in gliomas had a behavior inverse to that observed in controls, with a decrease (about 50%) in the cytosol and a marked increase (380%) in the particulate fraction, indicating a redistribution of enzyme activity.
Centro Interdipartimentale per la Ricerca Biomedica L.I.T.A. di Vialba, Milan, Milano, Italy.
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Tunici
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Sessa
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Rabellotti
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Calloni
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Perin
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology; D001932:Brain Neoplasms / Q000139:chemically induced / Q000201:enzymology*; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000008:administration & dosage; D003600:Cytosol / Q000201:enzymology; D005038:Ethylnitrosourea / Q000008:administration & dosage; D005260:Female; D005910:Glioma / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors; D011503:Transglutaminases / Q000378:metabolism*
D002273:Carcinogens; D011503:Transglutaminases; D005038:Ethylnitrosourea