Serological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci.
A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Réunion and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.
Hubert
K
K
Institut für Hygiene und Mikrobiologie, Universität Würzburg, 97080 Würzburg, Germany.
Andriantsimahavandy
A
A
Michault
A
A
Frosch
M
M
Mühlschlegel
F A
FA
D000818:Animals; D000947:Antigens, Helminth; D002999:Clone Cells / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology*; D018076:DNA, Complementary / Q000145:classification; D004797:Enzyme-Linked Immunosorbent Assay; D015723:Gene Library; D006801:Humans; D011994:Recombinant Proteins; D012698:Serologic Tests; D013621:Taenia / Q000235:genetics / Q000276:immunology*
D000947:Antigens, Helminth; D018076:DNA, Complementary; D011994:Recombinant Proteins