Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes.
We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy.
Colosimo
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Dipartimento di Biopatologia e Diagnostica per Immagini, Università di Roma 'Tor Vergata' and Istituto CSS-Mendel, Roma, Via di Tor Vergata 135, 00133, Rome, Italy.
Serafino
A
A
Sangiuolo
F
F
Di Sario
S
S
Bruscia
E
E
Amicucci
P
P
Novelli
G
G
Dallapiccola
B
B
Mossa
G
G
D052160:Benzothiazoles; D002461:Cell Line, Transformed; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology; D003959:Diamines; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005456:Fluorescent Dyes; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009930:Organic Chemicals; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011804:Quinolines; D014132:Trachea / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D008081:Liposomes; D009930:Organic Chemicals; D010714:Phosphatidylethanolamines; D011804:Quinolines; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; C098022:SYBR Green I; C094877:dioleoyl phosphatidylethanolamine; D004247:DNA; D002784:Cholesterol