Differential regulation of vasopressin gene expression in the hypothalamus of endotoxin-treated 14-day-old rat.
The E. coli endotoxin 0111 B4, a lipopolysaccharide (LPS), in a dose of 200 ng/kg body weight/50 microl artificial cerebrospinal fluid (CSF) was given intracisternally to 14-day-old rats. Four hours later CSF, blood and urine were sampled, and consecutive brain sections from the hypothalamic area of the brain were prepared for in situ hybridization. The LPS treatment resulted in a significant (p<0.001) pleocytosis and an elevation of the protein content of the CSF. There were no changes observed in the chemical parameters of the CSF, plasma, blood or urine, i.e. vasopressin (VP) levels, osmolality, Na+ and K+ concentrations, glucose level, pH, bicarbonate or PaCO2, PaO2 values. LPS injection, however, resulted in a significantly (p<0.01) increased VP mRNA level (121% of the control value) in the supraoptic nuclei (SON), but not in the paraventricular nuclei (PVN), as compared to controls. Our findings suggest an early effect of LPS on VP gene expression selectively in the SON of 14-days-old rats. This animal model might be suitable for studying the regulation of VP gene expression and the role of this peptide in the pathogenesis of bacterial meningitis in pediatric patients.
Department of Zoology and Cell Biology, University of Szeged, Hungary.
A
A
Boros
P
P
Temesvári
L
L
Szöke
M
M
Vecsernyés
F
F
Bari
C S
CS
Abrahám
S
S
Pintér
K
K
Gulya
D000818:Animals; D000831:Animals, Newborn; D017931:DNA Primers / Q000737:chemistry; D004731:Endotoxins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007032:Hypothalamus, Anterior / Q000187:drug effects* / Q000378:metabolism; D017403:In Situ Hybridization; D007276:Injections, Intraventricular; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014667:Vasopressins / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004731:Endotoxins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014667:Vasopressins; C044101:endotoxin, Escherichia coli