Identification of an HLA-A11 serological variant and its characterization by sequencing based typing.
We have identified an HLA-A11 variant allele, A*1105, segregating in a Caucasoid family. The variant antigen expressed by this allele failed to cross-react with most Caucasoid anti-HLA-A11 antisera tested. Sequencing based typing has been used to characterize this new allele and this showed that it has a novel mutation at a polymorphic position (502) in exon 3. In comparison with A*1101, the mutation (A-->G) results in an amino acid change from positively-charged lysine to negative glutamate and this may explain the altered HLA-A11 serological profile exhibited by this antigen. The new allele was found in a patient with acute lymphoid leukaemia (ALL), her father and two siblings.
Tissue Typing Laboratory, Manchester Blood Centre, Plymouth Grove, UK.
G
G
Morrell
J
J
Whalley
A
A
Stewart
S
S
Day
L
L
Lewis
Y
Y
Makar
S V
SV
Fuggle
J
J
Ross
P P
PP
Dunn
D000483:Alleles; D019943:Amino Acid Substitution; D005260:Female; D018698:Glutamic Acid; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology*; D059870:HLA-A11 Antigen; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008239:Lysine; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology*; D017422:Sequence Analysis, DNA; D044465:White People
D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D018698:Glutamic Acid; D008239:Lysine