Temporal, spatial and tissue-specific expression of a myogenin-lacZ transgene targeted to the Hprt locus in mice.
A lacZ transgene, expressed by the myogenin promoter, was introduced into the mouse hypoxanthine phosphoribosyltransferase (Hprt) locus by gene targeting in embryonic stem cells. Embryos between E10.5-E18.5 days were analyzed for expression of the transgene after staining for beta-galactosidase activity. Transgene expression was restricted to the skeletal muscle lineages reflecting a similar temporal and spatial pattern previously demonstrated for the endogenous myogenin gene. Additionally, a second transgene, MC1tk, showed expression in 87% of the clones when targeted to Hprt. This strategy, called targeted transgenesis, provides control for analyzing promoter sequences and for comparing various transgenes expressed by the same promoter.
Vivian
J L
JL
University of Texas M.D. Anderson Cancer Center, Houston, USA.
Klein
W H
WH
Hasty
P
P
D000818:Animals; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018390:Gene Targeting / Q000379:methods; D018259:Herpesvirus 1, Human / Q000201:enzymology / Q000235:genetics; D006651:Histocytochemistry; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism; D018008:Myogenin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013234:Stem Cells / Q000378:metabolism; D013937:Thymidine Kinase / Q000235:genetics; D014960:X Chromosome / Q000235:genetics
D009124:Muscle Proteins; C503801:Myog protein, mouse; D018008:Myogenin; D001086:Arabinofuranosyluracil; C043457:fialuridine; D007041:Hypoxanthine Phosphoribosyltransferase; D013937:Thymidine Kinase