Treatment of medullary thyroid carcinoma by combined expression of suicide and interleukin-2 genes.
Inherited medullary thyroid carcinomas (MTC) are aggressive and resistant to conventional chemo- and radiotherapies. We evaluated a novel strategy for treatment of MTC, combining "suicide" and interleukin-2 (IL-2) gene therapies. Tumors were produced in Wag/Rij rats by orthotopic injection of the rMTC 6-23 cell line, and/or derivatives expressing the herpes simplex virus 1 thymidine kinase (HSV1-TK) gene (rMTC-TK). Ganciclovir, a nucleoside analog selectively transformed to a toxic metabolite by HSV1-TK, totally eradicated rMTC-TK tumors in 60% of the animals. 1:1 rMTC and rMTC-TK mixed tumors were also strongly inhibited by ganciclovir (P < 0.05), indicating the occurrence of an efficient "bystander" effect in vivo. Double labelling of rMTC cell membranes and apoptotic nuclei revealed that, as with the TK+ cells, some TK- cells died by apoptosis. A 1:1 mixture of rMTC and rMTC-TK cells was administered to produce established tumors and then rMTC cells, transfected to express the IL-2 gene (rMTC-IL2), were inoculated. Combined ganciclovir and IL-2 treatment improved the inhibition of tumor growth compared to that following ganciclovir alone (86% compared to 54%, P < 0.05). This treatment also significantly enhanced macrophage activation and tumor infiltration by CD8+ and CD4+ T lymphocytes. These results open an avenue for combining suicide and immunoregulatory gene therapies for MTC management in man.
Laboratoire d'Endocrinologie Cellulaire et Evolution, Université Paris-Sud, Orsay, France.
M N
MN
Soler
G
G
Milhaud
F
F
Lekmine
F
F
Treilhou-Lahille
D
D
Klatzmann
S
S
Lausson
D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D018276:Carcinoma, Medullary / Q000276:immunology / Q000628:therapy*; D015774:Ganciclovir / Q000627:therapeutic use*; D015316:Genetic Therapy; D018259:Herpesvirus 1, Human / Q000201:enzymology*; D007376:Interleukin-2 / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology; D013937:Thymidine Kinase / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000276:immunology / Q000628:therapy*
D000998:Antiviral Agents; D007376:Interleukin-2; D013937:Thymidine Kinase; D015774:Ganciclovir