The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins.
Iron-sulfur (Fe/S) cluster-containing proteins catalyse a number of electron transfer and metabolic reactions. Little is known about the biogenesis of Fe/S clusters in the eukaryotic cell. Here, we demonstrate that mitochondria perform an essential role in the synthesis of both intra- and extra-mitochondrial Fe/S proteins. Nfs1p represents the yeast orthologue of the bacterial cysteine desulfurase NifS that initiates biogenesis by producing elemental sulfur. The matrix-localized protein is required for synthesis of both mitochondrial and cytosolic Fe/S proteins. The ATP-binding cassette (ABC) transporter Atm1p of the mitochondrial inner membrane performs an essential function only in the generation of cytosolic Fe/S proteins by mediating export of Fe/S cluster precursors synthesized by Nfs1p and other mitochondrial proteins. Assembly of cellular Fe/S clusters constitutes an indispensable biosynthetic task of mitochondria with potential relevance for an iron-storage disease and the control of cellular iron uptake.
Kispal
G
G
Institut für Zytobiologie und Zytopathologie der Philipps-Universität Marburg, Robert-Koch-Strasse 5, 35033 Marburg, Germany.
Csere
P
P
Prohl
C
C
Lill
R
R
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000906:Antibodies; D001059:Apoproteins / Q000378:metabolism; D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology; D007425:Intracellular Membranes / Q000502:physiology; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis*; D008564:Membrane Potentials; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D024101:Mitochondrial Proteins; D008954:Models, Biological; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013466:Sulfurtransferases
C091561:ATM1 protein, S cerevisiae; D018528:ATP-Binding Cassette Transporters; D000906:Antibodies; D001059:Apoproteins; D005656:Fungal Proteins; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D013466:Sulfurtransferases; C115912:NFS1 protein, S cerevisiae