Regulation of c-myc and immunoglobulin kappa gene transcription by promoter-proximal pausing of RNA polymerase II.
In normal cells, the proto-oncogene c-myc is regulated by promoter-proximal pausing of RNA polymerase II (pol II). In Burkitt lymphoma cells, c-myc is chromosomally translocated to one of the three immunoglobulin (Ig) gene loci and its transcription is driven constitutively by Ig enhancers. Promoter-proximal pausing of pol II is abolished on the translocated c-myc allele. This raised the question whether induction of Ig gene transcription also involves activation of promoter-proximal paused pol II. Here we have studied the transcriptional activation of a functionally rearranged Ig kappa gene in the mouse pre B cell line 70Z/3. We show that pol II pauses approximately 50 bp downstream of the transcriptional start site of the uninduced Ig kappa gene.
Institute for Clinical Molecular Biology and Tumor Genetics, GSF-Research Center for Environment and Health, Munich, Germany.
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Schneider
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Albert
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Wolf
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Eick
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D005786:Gene Expression Regulation; D016259:Genes, myc; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008954:Models, Biological; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic
D007145:Immunoglobulin kappa-Chains; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012319:RNA Polymerase II