Differential regulation of nitric oxide synthase-II mRNA by growth factors in rat, bovine, and human retinal pigmented epithelial cells.
Retinal pigmented epithelial cells (RPE cells) express the inducible isoform of NO synthase (NOS-II) after stimulation by cytokines and endotoxin. We have attempted to describe and compare the effect of fibroblast growth factor (FGF) and transforming growth factor beta (TGFbeta) on NOS-II mRNA level in three different cell species: rat, bovine, and human. Northern blot analysis demonstrated that, in the rat RPE cells, FGF upregulates and TGFbeta inhibits NOS-II mRNA accumulation, whereas in the bovine cells, the opposite effect appears. For the human RPE cells, RT-PCR analysis demonstrated that FGF upregulates and TGFbeta inhibits NOS-II mRNA accumulation, as in rat cells, even though the effect of TGFbeta was weaker. Thus, this study demonstrates that marked differences in growth factors regulating NOS-II occur between species, suggesting fundamental signal transduction differences at some level between rat, human, and cow.
Développement, Vieillissement et Pathologie de la Rétine, U450, Institut National de la Santé et de la Recherche Médicale, Paris, France.
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Faure
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Courtois
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Goureau
D000818:Animals; D015152:Blotting, Northern; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012486:Salmonella typhimurium; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D015854:Up-Regulation
D016207:Cytokines; D017931:DNA Primers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat