Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture.
Transplantation of osteogenic cells with a suitable matrix is one strategy for engineering bone tissue. Three-dimensional distribution and growth of cells within the porous scaffold are of clinical significance for the repair of large bony defects. A nano-HAp/collagen (nHAC) composite that mimics the natural bone both in composition and microstructure to some extent was employed as a matrix for the tissue engineering of bone. A porous nHAC composite was produced in sheet form and convolved to be a three-dimensional scaffold. Using organ culture techniques and the convolving method, we have developed three-dimensional osteogenic cells/nHAC constructs in vitro. Scanning electron microscopic and histological examination has demonstrated the development of the cells/material complex. Spindle-shaped cells migrating out of bone fragments continuously proliferated and migrated throughout the network of the coil. The porous nHAC scaffold provided a microenvironment resembling that seen in vivo, and cells within the composite eventually acquired a tridimensional polygonal shape. In addition, new bone matrix was synthesized at the interface of bone fragments and the composite.
Du
C
C
Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China.
Cui
F Z
FZ
Zhu
X D
XD
de Groot
K
K
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes; D002455:Cell Division; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques / Q000379:methods*; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology* / Q000637:transplantation; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite