Protective role of ubiquinone in vitamin E and selenium-deficient plasma membranes.
We have studied the effects of dietary depletion of vitamin E and selenium on endogenous ubiquinone-dependent antioxidant system. Deficiency induced an increase in both coenzyme Q9 and Q10 in liver tissue, reaching a maximum between 4 and 7 weeks of deficient diet consumption. Cytochrome b5 reductase polypeptide was also enriched in membranes after 5 weeks of deficient diet consumption. Substantial DT-diaphorase activity was found in deficient, but not in control plasma membranes. Deficient membranes were very sensitive to lipid peroxidation, although a great protection was observed after incubation with NAD(P)H. Our results show that liver cells can boost endogenous ubiquinone-dependent protective mechanisms in response to deficiency in vitamin E and selenium.
Departamento de Biología Celular, Universidad de Córdoba, Spain.
F
F
Navarro
A
A
Arroyo
S F
SF
Martín
R I
RI
Bello
R
R
de Cabo
J R
JR
Burgess
P
P
Navas
J M
JM
Villalba
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D003067:Coenzymes; D003579:Cytochrome Reductases / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004579:Electron Transport; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D012643:Selenium / Q000172:deficiency* / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism* / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000378:metabolism*
D003067:Coenzymes; D009243:NAD; D014451:Ubiquinone; D014810:Vitamin E; D009249:NADP; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase; D008058:Dihydrolipoamide Dehydrogenase; C024989:coenzyme Q10; D012643:Selenium; C030571:ubiquinone 9