A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds.
For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.
Leiner
G
G
Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, 30173 Hannover, Germany.
Franz
B
B
Strutzberg
K
K
Gerlach
G F
GF
D016977:Actinobacillus pleuropneumoniae / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006460:Hemolysin Proteins; D011001:Pleuropneumonia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis*
D000942:Antigens, Bacterial; C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins