Deprivation of endogenous brain-derived neurotrophic factor results in impairment of spatial learning and memory in adult rats.
Brain-derived neurotrophic factor (BDNF) is abundantly expressed in the hippocampus and cerebral cortex and is involved in synaptic plasticity and long-term potentiation (LTP). The present study was under taken to investigate whether endogenous BDNF was required for spatial learning and memory in a rat model. Antibodies to BDNF (anti-BDNF, n=7) or control immunoglobulin G (control, n=6) were delivered into the rat brain continuously for 7 days with an osmotic pump. The rats were then subjected to a battery of behavioral tests. The results show that the average escape latencies in the BDNF antibody treated group were dramatically longer than those of the control (F=13.3, p<0.001). The rats treated with control IgG swam for a significantly longer distance in the P quadrant (where the escape plane had been placed) compared with the other three quadrants (p<0.05). In contrast, anti-BDNF-treated rats swam an equivalent distance in all four quadrants. The average percentage of swimming distance in the P quadrant by anti-BDNF-treated rats was much less than that by control IgG treated rats (p<0.001). These results suggest that endogenous BDNF is required for spatial learning and memory in adult rats.
Department of Neurology, Nanfang Hospital, First Military Medical University, Guangzhou, 510515, China.
J S
JS
Mu
W P
WP
Li
Z B
ZB
Yao
X F
XF
Zhou
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000172:deficiency*; D003463:Cues; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008569:Memory Disorders / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013037:Spatial Behavior / Q000502:physiology*; D013550:Swimming / Q000502:physiology
D019208:Brain-Derived Neurotrophic Factor