Genetic organization of the citCDEF locus and identification of mae and clyR genes from Leuconostoc mesenteroides.
In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the citrate lyase locus revealed one polycistronic mRNA covering the mae and citCDEF genes. This transcript was detected only on RNA prepared from cells grown in the presence of citrate. Primer extension experiments suggest that clyR and the citrate lyase operon are expressed from a bidirectional A-T-rich promoter region located between mae and clyR.
Bekal-Si Ali
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Laboratoire de Microbiologie, UA INRA, Université de Bourgogne ENS. BANA, F-21 000 Dijon, France.
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D019343:Citric Acid / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007934:Leuconostoc / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics*; D008293:Malates / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C120333:ClyR protein, Leuconostoc mesenteroides; D008293:Malates; D009097:Multienzyme Complexes; D019343:Citric Acid; C030298:malic acid; D008291:Malate Dehydrogenase; C046446:malate dehydrogenase-(oxaloacetate-decarboxylating) (NAD+); D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase