Urokinase-type plasminogen-activator and normal thyroid cell adhesion to the extracellular matrix.
The urokinase-type plasminogen activator receptor (uPA-R) focuses the proteolytic activity of its ligand, the urokinase-type plasminogen activator (uPA), on the cell surface, and can also act as an adhesion receptor for vitronectin (VTN). uPA increases uPA-R affinity for VTN and is also able to cleave its receptor. We have previously shown that uPA-R is involved in the adhesion of normal thyroid cells to VTN. In the present report, we have investigated the effect of uPA on normal thyroid cell adhesion to some extracellular matrix (ECM) components. We show that a short-term treatment with uPA does not change normal thyroid cell adhesion to fibronectin (FNT), collagen (CGN), laminin (LMN) and VTN. The prolongation of uPA treatment increases cell adhesion to VTN, and, less efficiently, to other ECM components. Since the short term uPA treatment causes a partial cleavage of uPA-R, that does not increase with time, the observed increase in cell adhesivity cannot be related to the cleavage of uPA-R. We show that the adhesion improvement after the long term uPA treatment is instead due to a strong increase of the cell-surface expression of the integrin beta3 and a moderate increase of the integrin alpha(v). Both alpha(v) beta3 and alpha(v) beta1 are integrinic receptors for VTN.
Centro di Endocrinologia ed Oncologia Sperimentale, Consiglio Nazionale delle Ricerche, Naples, Italy.
P
P
Ragno
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Montuori
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S
Salzano
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G
Rossi
D015703:Antigens, CD / Q000096:biosynthesis; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D039564:Integrin alphaV; D019012:Integrin beta1 / Q000096:biosynthesis; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism* / Q000494:pharmacology
D015703:Antigens, CD; D039564:Integrin alphaV; D019012:Integrin beta1; D039661:Integrin beta3; C524898:PLAUR protein, human; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator