Clinical applications of gas chromatography and gas chromatography-mass spectrometry of steroids.
This review article underlines the importance of gas chromatography-mass spectrometry (GC-MS) for determination of steroids in man. The use of steroids labelled with stable isotopes as internal standard and subsequent analysis by GC-MS yields up to now the only reliable measurement of steroids in serum. Isotope dilution GC-MS is the reference method for evaluation of routine analysis of serum steroid hormones. GC-MS is an important tool for detection of steroid hormone doping and combined with a combustion furnace and an isotope ratio mass spectrometer the misuse of testosterone by athletes can be discovered. Finally the so called urinary steroid profile by GC and GC-MS is the method of choice for detection of steroid metabolites in health and disease.
Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
B G
BG
Wolthers
G P
GP
Kraan
D045930:Anabolic Agents / Q000032:analysis; D000728:Androgens / Q000032:analysis; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007201:Indicator Dilution Techniques; D013256:Steroids / Q000032:analysis* / Q000652:urine; D009626:Terminology as Topic
D045930:Anabolic Agents; D000728:Androgens; D013256:Steroids