Ploidy regulation of gene expression.
Microarray-based gene expression analysis identified genes showing ploidy-dependent expression in isogenic Saccharomyces cerevisiae strains that varied in ploidy from haploid to tetraploid. These genes were induced or repressed in proportion to the number of chromosome sets, regardless of the mating type. Ploidy-dependent repression of some G1 cyclins can explain the greater cell size associated with higher ploidies, and suggests ploidy-dependent modifications of cell cycle progression. Moreover, ploidy regulation of the FLO11 gene had direct consequences for yeast development.
Galitski
T
T
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
Saldanha
A J
AJ
Styles
C A
CA
Lander
E S
ES
Fink
G R
GR
D002688:Chitinases / Q000235:genetics; D016213:Cyclins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D016193:G1 Phase; D015966:Gene Expression Regulation, Fungal; D006238:Haploidy; D008074:Lipoproteins / Q000235:genetics / Q000502:physiology; D000072235:Mating Factor; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000235:genetics / Q000502:physiology; D010675:Pheromones; D011003:Ploidies; D011123:Polyploidy; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics
C082719:CLN1 protein, S cerevisiae; D016213:Cyclins; C110690:FLO11 protein, S cerevisiae; D005656:Fungal Proteins; D008074:Lipoproteins; C095063:MFA2 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; D010675:Pheromones; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C070839:PCL1 protein, S cerevisiae; D000072235:Mating Factor; D002688:Chitinases