Genome analysis of Marek's disease virus strain CVI-988: effect of cell culture passage on the inverted repeat regions.
The genomes of different derivatives of Marek's disease virus (MDV) strain CVI-988, a low oncogenic isolate of a serotype 1 MDV, were analyzed by restriction enzyme analyses to detect whether alterations occurred after passages in cell culture. DNA molecules of strain 988 isolated directly from blood cells contained mainly two copies of the 132-bp repeat sequence previously reported within BamH1-H and -D fragment as previously reported for more virulent MDV strains. Although a minority of virus particles showed repeat amplification was already at the fifth passage level, amplification mainly occurred between passages 17 and 34 in cell culture. In addition, a 400-bp deletion was detected within the BamH1-A fragment of two derivatives of CVI-988, 988C and 988C/R6.
Institute for Animal Science and Health (ID-DLO), Department of Avian Virology, Lelystad, The Netherlands.
B J
BJ
van Iddekinge
L
L
Stenzler
K A
KA
Schat
H
H
Boerrigter
G
G
Koch
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D008380:Marek Disease / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000821:virology; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping / Q000662:veterinary
D004279:DNA, Viral