In ovo vaccination of specific-pathogen-free chickens with vaccines containing multiple agents.
We used in ovo technology to protect chickens against multiple diseases by inoculating vaccines containing mixtures of live viral agents. A single in ovo injection of a vaccine containing serotypes 1, 2, and 3 of Marek's disease virus (MDV), a vaccine strain of serotype 1 infectious bursal disease virus (IBDV), and recombinant fowl pox vaccine with HN and F genes of Newcastle disease virus (rFP-NDV) induced protection against virulent MDV, IBDV, Newcastle disease virus, and fowl poxvirus. The multiple-agent vaccine induced specific antibodies against the viral agents present in the mixture and did not adversely affect the survival of hatched chickens. Inoculation of a vaccine containing serotypes 1, 2, and 3 of MDV and IBDV did not affect hatchability of eggs, although the addition of rFP-NDV to the mixture reduced hatchability by 23%-26%. In ovo vaccination with a vaccine containing MDV and IBDV vaccine viruses did not exacerbate the inhibitory effect of individual viral agents on humoral and cellular immune competence.
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
M
M
Gagic
C A
CA
St Hill
J M
JM
Sharma
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002645:Chickens / Q000276:immunology*; D005587:Fowlpox virus / Q000276:immunology*; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D010063:Ovum / Q000821:virology*; D011201:Poultry Diseases / Q000517:prevention & control*; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D017778:Vaccines, Combined; D014765:Viral Vaccines