Sequence and expression analysis of a novel Xenopus laevis cDNA that encodes a protein similar to bacterial and chloroplast ribosomal protein L24.
We report here the cloning and the characterization of a Xenopus laevis cDNA that encodes a basic protein of 276 amino acids with a central core region, which shows a substantial degree of homology to bacterial and chloroplast ribosomal protein L24, and additional diverged N- and C-terminal polypeptide extensions. The N-terminal extension displays similarities to the mitochondrial targetting sequence, thereby suggesting that the cDNA probably codes for a mitochondrial ribosomal protein. Although the gene was expressed ubiquitously, at fairly constant levels, during embryogenesis, the abundance of the transcripts in the different tissues varies with the mRNA levels in the kidney, adipose tissue, muscle and liver being greater than that present in the brain, heart, ovary and lung.
Cardiff School of Biosciences, Cardiff University, PO Box 911, Cardiff CF1 3US, UK.
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Kousteni
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Tura-Kockar
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Ramji
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002736:Chloroplasts / Q000737:chemistry*; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008928:Mitochondria / Q000737:chemistry; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
D001426:Bacterial Proteins; D018076:DNA, Complementary; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C036298:ribosomal protein L24