Fast excitatory post synaptic potentials and their response to catecholaminergic antagonists in rat sympathetic preganglionic neurones in vitro.
In an in vitro slice preparation from neonatal rats intracellular recordings were made from electrophysiologically identified sympathetic preganglionic neurones. Electrical stimulation in the lateral funiculus (>500 microm) from the recording site elicited a mono- or polysynaptic excitatory post synaptic potential. The latter potential was blocked with the dopamine D2 antagonist haloperidol but not with the dopamine D1 antagonist SCH 23390. We therefore report the first showing of a functional descending pathway in an in vitro slice preparation describing both the transmitter and the receptor subtype involved and physiologically show that dopamine may exert an indirect excitatory influence on sympathetic preganglionic neurones possibly via interneurones present in the spinal cord.
Gladwell
S J
SJ
Department of Physiology, The Medical School, University of Birmingham, Edgbaston, UK. s.j.gladwell@bham.ac.uk
Coote
J H
JH
D001339:Autonomic Fibers, Preganglionic / Q000187:drug effects* / Q000502:physiology; D002395:Catecholamines / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D006220:Haloperidol / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000502:physiology
D002395:Catecholamines; D006220:Haloperidol; D004298:Dopamine