Transcriptional activation by Arabidopsis GT-1 may be through interaction with TFIIA-TBP-TATA complex.
GT-1 belongs to the class of trihelix DNA-binding proteins and binds to a promoter sequence found in many different genes. Data presented in this report show that GT-1 contains a trans-activation function in yeast and in plant cells. However, in tobacco BY-2 protoplasts, this activity functions only when an internal region containing the DNA-binding domain is deleted. Gel-shift and co-immunoprecipitation assays have revealed that GT-1 can interact with and stabilize the TFIIA-TBP-TATA complex. These results suggest that GT-1 may activate transcription through direct inter- action with the transcriptional pre-initiation complex.
Le Gourrierec
J
J
Laboratoire de Génétique Moléculaire des Plantes, Université de Picardie Jules Verne, Amiens, France.
Li
Y F
YF
Zhou
D X
DX
D017360:Arabidopsis / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010947:Plants, Toxic; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; C077926:GT-1 transcription factor; D009687:Nuclear Proteins; D014157:Transcription Factors