Disruption and basic functional analysis of six novel ORFs of chromosome XV from Saccharomyces cerevisiae.
We report the disruption and functional analysis of six open reading frames (ORFs) from chromosome XV, namely YOL155c, YOL154w, YOL119c, YOL118c, YOR301w and YOR306c, in FY1679 and CEN.PK2 backgrounds. We constructed replacement cassettes and cloned each ORF into the pRS416 centromeric plasmid. No obvious phenotype was observed for the corresponding deleted strains with respect to growth, mating or sporulation. YOL155c encodes a protein with a secretion signal and putative GPI-anchor recognition site and is possibly a cell wall protein, although its deletion did not present morphogenetic defects under any of the conditions tested. Although YOL119c and YOR306c are members of the monocarboxylate permease family, the growth of the double disruptant in acetate, lactate and pyruvate was similar to that of the parental strains.
Instituto de Investigaciones Biomédicas Alberto Sols, C.S.I.C., E-28029 Madrid, Spain.
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D000595:Amino Acid Sequence; D000946:Antigens, Fungal / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D016415:Sequence Alignment
D000946:Antigens, Fungal; D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins