Rapid chiral high-performance liquid chromatographic assay for salmeterol and alpha-hydroxysalmeterol. Application to in vitro metabolism studies.
A rapid and sensitive chiral high-performance liquid chromatographic (HPLC) assay for the simultaneous determination of salmeterol and its principal human metabolite alpha-hydroxysalmeterol is described. The two pairs of enantiomers were resolved on a chiral-cellobiohydrolase column and detected by electrochemical detection at +700 mV. Standard curves were linear over the concentration range 0.1 to 4.0 microM for alpha-hydroxysalmeterol enantiomers and 2.5 to 40.0 microM for salmeterol enantiomers. Intra- and inter-day coefficients of variation were < 10%. The method was applied to a study of human hepatic metabolism in vitro which showed that microsomal metabolism of salmeterol to alpha-hydroxysalmeterol is not stereoselective.
School of Pharmacy, University of Otago, Dunedin, New Zealand.
M
M
Zhang
J P
JP
Fawcett
J P
JP
Shaw
D000420:Albuterol / Q000031:analogs & derivatives* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D008862:Microsomes, Liver / Q000378:metabolism; D000068299:Salmeterol Xinafoate; D013237:Stereoisomerism
C101433:alpha-hydroxysalmeterol; D000068299:Salmeterol Xinafoate; D000420:Albuterol