Analysis of fliC variation among clinical isolates of Burkholderia cepacia.
PCR and restriction fragment length polymorphism (RFLP) typing of flagellin genes (fliC) from 57 clinical isolates of Burkholderia cepacia indicated that only type 11 flagellins were present. Twenty-two isolates previously identified as the epidemic UK cystic fibrosis strain were indistinguishable by this method, as were 11 isolates from a pseudo-outbreak in Senegal. Other clinical isolates, including 19 from disparate sources in Malaysia, were separated into nine fliC RFLP groups, exhibiting a large degree of divergence. When isolates were indistinguishable by fliC genotyping, their similarity was confirmed by whole genome macro-restriction analysis with pulsed-field gel electrophoresis following XbaI digestion. The variation in fliC sequences of B. cepacia was far greater than that with B. pseudomallei, supporting the view that 'B. cepacia', as currently defined, may comprise several different genomic species.
Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire BD7 1DP.
Craig
C
Winstanley
Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire BD7 1DP.
Barbara A
BA
Hales
Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF.
J Alun W
JAW
Morgan
Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, PO Box 147, Liverpool L69 3BX.
Malcolm J
MJ
Gallagher
Department of Medical Microbiology, University of Malaya, Kuala Lumpur 50603, Malaysia.
Svithri D
SD
Puthucheary
Department of Medical Microbiology, Hospital d'Enfants, Albort Royer, Dakar, Senegal.
Moussa F
MF
CISSé
Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, PO Box 147, Liverpool L69 3BX.
C Anthony
CA
Hart
D019121:Burkholderia Infections / Q000150:complications*; D016956:Burkholderia cepacia / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D003550:Cystic Fibrosis / Q000150:complications*; D017931:DNA Primers / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D016521:Electrophoresis, Gel, Pulsed-Field; D005408:Flagellin / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008296:Malaysia; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000737:chemistry; D012675:Senegal; D006113:United Kingdom
D017931:DNA Primers; D012329:RNA, Bacterial; D005408:Flagellin; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific