Upstream and downstream sequence elements determine the specificity of the rice tungro bacilliform virus promoter and influence RNA production after transcription initiation.
The contribution of sequences upstream and downstream of the transcription start site to the strength and specificity of the promoter of rice tungro bacilliform virus was analysed in transgenic rice plants. The promoter is strongly stimulated by downstream sequences which include an intron and is active in all vascular and epidermal cells. Expression in the vascular tissue requires a promoter element located between -100 and -164 to which protein(s) from rice nuclear extracts bind. Elimination of this region leads to specificity for the epidermis. Due to the presence of a polyadenylation signal in the intron, short-stop RNA is produced from the promoter in addition to full-length primary transcript and its spliced derivatives. The ratio between short-stop RNA and full-length or spliced RNA is determined by upstream promoter sequences, suggesting the assembly of RNA polymerase complexes with different processivity on this promoter.
Institute of Plant Sciences, ETH Zürich, Switzerland.
A
A
Klöti
C
C
Henrich
S
S
Bieri
X
X
He
G
G
Chen
P K
PK
Burkhardt
J
J
Wünn
P
P
Lucca
T
T
Hohn
I
I
Potrykus
J
J
Fütterer
D019195:Badnavirus / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D005966:Glucuronidase / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D014018:Tissue Distribution; D014158:Transcription, Genetic
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase