Al2O3 doped apatite-wollastonite containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells.
Fresh marrow cells were obtained from femora of Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) until confluence. After trypsinization, cells were subcultured at a cell density of 100 x 10(3)/35-mm well in the presence of FCS, beta-glycerophosphate, and ascorbic acid phosphate on four different culture substrata. The period of subculture was 2 weeks; the substrata used were the culture dish, apatite-wollastonite containing glass ceramic (AW), hydroxyapatite coated AW (HA/AW), and Al2O3 doped AW (Al/AW). The HA coating was attained by the incubation of AW in simulated physiological solution. The glass matrix of AW and HA/AW contained MgO, CaO, P2O5, and SiO2; Al/AW contained Al2O3 in addition to these components. The subculture on Al/AW substratum showed many alkaline phosphatase (ALP) positive nodules and the highest ALP activity. On a Northern blot analysis the housekeeping gene of beta-actin mRNA was evenly detected from the cells cultured on all substrata; however, bone-specific osteocalcin mRNA was only detected from the cells on Al/AW. These results indicate that Al/AW provokes the osteoblastic differentiation of marrow stromal stem cells.
Department of Orthopedics, Nara Medical University, Nara 634, Japan. hajimeoh@naramed-u.ac.jp
H
H
Ohgushi
T
T
Yoshikawa
H
H
Nakajima
S
S
Tamai
Y
Y
Dohi
K
K
Okunaga
D000469:Alkaline Phosphatase / Q000032:analysis; D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D017610:Calcium Compounds / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D017886:Durapatite / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects*; D019736:Prostheses and Implants; D051381:Rats; D011916:Rats, Inbred F344; D017640:Silicates / Q000737:chemistry*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D001031:Apatites; D001672:Biocompatible Materials; D015415:Biomarkers; D017610:Calcium Compounds; D017640:Silicates; D015675:Osteocalcin; C023506:Glass ceramics; D004247:DNA; D017886:Durapatite; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide; C031293:calcium silicate