Eliciting an immune response by plasmid DNA encoding a human sperm protein (HSD-1).
The cDNA encoding a human sperm membrane designated as HSD-1 was isolated from a human testis lambda gt11 cDNA expression library and assigned the accession number U12978 by GenBank. HSD-1 was conjugated to an eukaryotic expression plasmid (pRSV) to construct the recombinant plasmid pRSV-HSD-1. Female mice were inoculated intramuscularly with the plasmid DNA and the expression of HSD-1 was determined. HSD-1 mRNAs were detected in myocytes and endomysial connective tissue cells of the quadriceps muscle by in situ hybridization. Spleen of inoculated animals contained an increased number of cytotoxic T lymphocytes, phagocytes, and plasma cells. Fertility of the treated animals was not affected. Thus, intramuscular inoculation of female mice with the plasmid DNA (pRSV-HSD-1) results in the expression of HSD-1 and may elicit a tissue-mediated immune response.
Wu
Y W
YW
National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Chen
D H
DH
Miao
S Y
SY
Wang
L F
LF
Zong
S D
SD
Koide
S S
SS
D000818:Animals; D000954:Antigens, Surface; D003238:Connective Tissue / Q000378:metabolism; D005260:Female; D005298:Fertility; D018014:Gene Transfer Techniques; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D010586:Phagocytes / Q000166:cytology; D010957:Plasmids / Q000276:immunology*; D013094:Spermatozoa / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D014158:Transcription, Genetic
D000954:Antigens, Surface; D008565:Membrane Proteins; C099014:SPAG8 protein, human