Control of attachment, morphology, and proliferation of skeletal myoblasts on silanized glass.
Generating skeletal muscle in vitro is an attractive approach to overcome problems associated with autologous transfer of muscle and donor site morbidity during plastic surgery. Such tissue engineering requires application of biomaterials that selectively control the attachment, morphology, and proliferation of muscle progenitor ("satellite") cells. This study examined the initial attachment, morphological characteristics, and proliferative behavior of murine C2C12 myoblasts on glass substrata derivatized with self-assembled monolayers (SAMs) of the organosiloxanes N-(2-aminoethyl)(3-aminopropyl)trimethoxysilane (EDA) and tridecafluoro-1,1,2,2-tetrahydrooctyl-1-dimethylchlorosil ane (13F). The fraction of myoblasts resisting detachment upon rinsing was greater on EDA than on 13F. Application of a quantitative moments-based analysis of cell morphology demonstrated that projected area and two size-invariant metrics of shape (extension and dispersion) for these cells were greater for EDA than for 13F. Myoblasts also proliferated faster on EDA than on 13F. These data indicate that EDA-derivatized glass provides a superior substratum for myoblast culture compared to 13F-derivatized glass. Understanding myoblast behavior on these biomaterials that promotes contrasting cellular responses is the first step toward using patterned SAMs to control myotube alignment for tissue engineering skeletal muscle.
Division of Plastic, Reconstructive, and Maxillofacial Surgery, University of Pittsburgh Medical Center, Pennsylvania 15261, USA.
T O
TO
Acarturk
M M
MM
Peel
P
P
Petrosko
W
W
LaFramboise
P C
PC
Johnson
P A
PA
DiMilla
D000473:Alkanes / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002455:Cell Division; D016764:Cell Polarity; D048429:Cell Size; D002478:Cells, Cultured; D005898:Glass / Q000737:chemistry; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D012821:Silanes / Q000737:chemistry; D012826:Silicone Elastomers / Q000737:chemistry; D017155:Wettability
D000473:Alkanes; D012821:Silanes; D012826:Silicone Elastomers; C410437:tridecafluoro-1,1,2,2-tetrahydrooctyl-1-dimethylchlorosilane; C006194:aminoethyl-aminopropyl-trimethoxysilane