Application of a new high sensitivity luminometer for industrial microbiology and molecular biology.
A compact new luminometer (FB12) has been developed based on a 370-630 nm photon counter and measuring chamber that can accommodate a range of sample formats. The FB12 permits measurements as low as 1000 molecules of luciferase in reporter gene assays. Its sensitivity for ATP is limited by reagent background. If ATP assay reagents had no chemical background, 2 fg of ATP could be detected using 3 SD of instrument background as the detection limit. The FB12 has a dynamic range of six decades and operates under its own microprocessor programme or protocol-based PC software that is integrated with Microsoft(R) Excel(R). An injector port above the sample measuring position allows connection of external reagent injectors. Applications are performed using protocols provided with the FB12 or user defined protocols. Examples are presented that illustrate use of the instrument for research and industrial applications.
Andreotti
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ASD, Inc., 4320 NE 25th Avenue, Fort Lauderdale, FL 33308, USA.
Berthold
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D007221:Industry; D008156:Luciferases / Q000032:analysis*; D049449:Luminescence; D008828:Microbiological Techniques / Q000295:instrumentation*; D008967:Molecular Biology / Q000295:instrumentation* / Q000379:methods; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity
D008156:Luciferases