Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis.
Enzymes from hyperthermophiles can be efficiently purified after expression in mesophilic hosts and are well-suited for crystallisation attempts. Two enzymes of histidine biosynthesis from Thermotoga maritima, N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase and the cyclase moiety of imidazoleglycerol phosphate synthase, were overexpressed in Escherichia coli, both in their native and seleno-methionine-labelled forms, purified by heat precipitation of host proteins and crystallised. N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase crystallised in four different forms, all suitable for X-ray structure solution, and the cyclase moiety of imidazoleglycerol phosphate synthase yielded one crystal form that diffracted to atomic resolution. The obtained crystals will enable the determination of the first three-dimensional structures of enzymes from the histidine biosynthetic pathway.
Abteilung für Biophysikalische Chemie, Biozentrum der Universität Basel, Switzerland.
R
R
Thoma
G
G
Obmolova
D A
DA
Lang
M
M
Schwander
P
P
Jenö
R
R
Sterner
M
M
Wilmanns
D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000302:isolation & purification*; D000619:Aminohydrolases / Q000737:chemistry* / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D018360:Crystallography, X-Ray; D006639:Histidine / Q000096:biosynthesis*; D013058:Mass Spectrometry
D001426:Bacterial Proteins; D006639:Histidine; C087227:imidazole glycerol phosphate synthase; D000619:Aminohydrolases; D019747:Aldose-Ketose Isomerases; C079656:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase