Possible role of cardiac mast cells in norepinephrine-induced myocardial preconditioning.
The present study was designed to investigate the role of cardiac mast cells in the cardioprotective effect of norepinephrine-induced preconditioning. Isolated rat heart was subjected to 30 min of global ischemia followed by 30 min of reperfusion. Both ischemic and norepinephrine (100 microM) preconditioning markedly reduced ischemia-reperfusion-induced release of lactate dehydrogenose (LDH) in the coronary effluent and the incidence of ventricular premature beats (VPBs) and ventricular tachycardia/fibrillation (VT/VF) during the reperfusion phase. Ischemic and norepinephrine preconditioning also significantly reduced ischemia-reperfusion-induced release of mast cell peroxidase (MPO), a marker of mast cell degranulation. However, MPO release increased immediately after ischemic or norepinephrine preconditioning. Histological study with ruthenium red (0.005%) staining confirmed cardiac mast cell degranulation in ischemic and norepinephrine preconditioned isolated rat hearts. These findings tentatively suggest that pharmacological preconditioning with norepinephrine produces a cardioprotective and antiarrhythmic effect similar to ischemic preconditioning through degranulation of resident cardiac mast cells.
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, India.
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Parikh
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Singh
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000517:prevention & control; D015550:Cell Degranulation / Q000187:drug effects; D004562:Electrocardiography / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008407:Mast Cells / Q000502:physiology*; D009206:Myocardium / Q000166:cytology* / Q000473:pathology; D009638:Norepinephrine / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012430:Ruthenium Red / Q000737:chemistry; D018879:Ventricular Premature Complexes / Q000517:prevention & control
D012430:Ruthenium Red; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D009638:Norepinephrine