CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions.
BACKGROUND
CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified.


OBJECTIVE
We sought to determine the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases.


METHODS
We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific T-T cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86(+) T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects.


RESULTS
On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86(+) T cells in primary culture from atopic patients was significantly higher than that from control subjects.


CONCLUSIONS
These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.
Nakada
M
M
Department of Otorhinolaryngology and the Department of Pathology, Okayama University Medical School, Okayama, Japan.
Nishizaki
K
K
Yoshino
T
T
Okano
M
M
Yamamoto
T
T
Masuda
Y
Y
Ohta
N
N
Akagi
T
T
D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000502:physiology*; D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000502:physiology*; D051940:B7-2 Antigen; D002450:Cell Communication; D004391:Dust; D000939:Epitopes; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000473:pathology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000502:physiology*; D008925:Mites / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation
D000485:Allergens; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D004391:Dust; D000939:Epitopes; D008562:Membrane Glycoproteins