Solution structure of core binding factor beta and map of the CBF alpha binding site.
The core binding factor beta subunit (CBF beta) is the non-DNA binding subunit of the core-binding factors, transcription factors essential for multiple developmental processes including hematopoiesis and bone development. Chromosomal translocations involving the human CBFB gene are associated with a large percentage of human leukemias. The N-terminal 141 amino acids of CBF beta contains the heterodimerization domain for the DNA-binding CBF alpha subunits, and is sufficient for CBF beta function in vivo. Here we present the high-resolution solution structure of the CBF beta heterodimerization domain. It is a novel alpha/beta structure consisting of two three-stranded beta-sheets packed on one another in a sandwich arrangement, with four peripheral alpha-helices. The CBF alpha binding site on CBF beta has been mapped by chemical shift perturbation analysis.
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22906, USA.
X
X
Huang
J W
JW
Peng
N A
NA
Speck
J H
JH
Bushweller
D001665:Binding Sites; D002942:Circular Dichroism; D050657:Core Binding Factor alpha Subunits; D050658:Core Binding Factor beta Subunit; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D017433:Protein Structure, Secondary; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000737:chemistry*
C493692:CBFB protein, human; D050657:Core Binding Factor alpha Subunits; D050658:Core Binding Factor beta Subunit; D004268:DNA-Binding Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors