Molecular cloning and functional characterization of a snake venom metalloprotease.
A cDNA clone, MT-d, encoding metalloprotease precursor was isolated from snake (Agkistrodon halys brevicaudus) venom gland cDNA library. MT-d-I protein containing both metalloprotease and disintegrin domains, and MT-d-II protein containing the metalloprotease domain only were expressed in Escherichia coli and refolded successfully into their functional forms. Each of the refolded enzyme species exhibited distinct substrate specificity. Proteolytic activity of the MT-d-1 was able to hydrolyse type I gelatin, type-III and V collagens in contrast with the catalytic function of MT-d-II. MT-d-I protein having metalloprotease activity was also able to inhibit platelet aggregation. Functionally active MT-d-I protein underwent autoproteolytic processing in vitro to produce metalloprotease and disintegrin; this processing was accompanied by significant changes in the substrate specificity of the enzyme activity. Experimental evidence strongly suggests that the disintegrin domain in the metalloprotease precursor modulates the catalytic function of the enzyme in hydrolysing extracellular matrix proteins.
Jeon
O H
OH
Department of Biochemistry, College of Science, and Bioproducts Research Center, Yonsei University, Seoul, Korea.
Kim
D S
DS
D000595:Amino Acid Sequence; D001483:Base Sequence; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms / Q000201:enzymology*
D016326:Extracellular Matrix Proteins; D010975:Platelet Aggregation Inhibitors; D011994:Recombinant Proteins; D012910:Snake Venoms; D005340:Fibrinogen; D008666:Metalloendopeptidases