Selective coupling of mouse brain metabotropic sigma receptor with recombinant Gi1.
Various sigma (sigma) ligands including (+)-pentazocine stimulated [35S]GTPgammaS binding in synaptic membranes from the mouse cerebellum. The (+)-pentazocine-stimulated [35S]GTPgammaS binding was blocked by the treatment of membranes with pertussis toxin (PTX), but completely recovered by the reconstitution of PTX-treated membranes with recombinant Gi1, but not with GoA. These findings suggest that metabotropic sigma receptors are selectively coupled to Gi1 protein.
Tokuyama
S
S
Department of Molecular Pharmacology and Neuroscience, Nagasaki University School of Pharmaceutical Sciences, Japan.
Hirata
K
K
Yoshida
A
A
Maruo
J
J
Matsuno
K
K
Mita
S
S
Ueda
H
H
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D001923:Brain Chemistry; D019281:Dimerization; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010423:Pentazocine / Q000494:pharmacology; D037342:Pertussis Toxin; D017480:Receptors, sigma / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D013462:Sulfur Radioisotopes / Q000378:metabolism; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D017480:Receptors, sigma; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D010566:Virulence Factors, Bordetella; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D037342:Pertussis Toxin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D010423:Pentazocine