Interactions of HasA, a bacterial haemophore, with haemoglobin and with its outer membrane receptor HasR.
The major mechanism by which bacteria acquire free or haemoglobin-bound haem involves direct binding of haem to specific outer membrane receptors. Serratia marcescens and Pseudomonas aeruginosa have an alternative system, which involves an extracellular haemophore, HasA, that captures free or haemoglobin-bound haem and shuttles it to a specific cell surface outer membrane receptor, HasR. Both haem-free (apoprotein) and haem-loaded (holoprotein) HasA bind to HasR, evidence for direct protein-protein interactions between HasA and HasR. HasA binding to HasR takes place in a tonB mutant. TonB is thus required for a step subsequent to HasA binding.
Unité des Membranes bactériennes (CNRS URA 1300), Institut Pasteur, Paris, France.
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Létoffé
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Nato
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Goldberg
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Wandersman
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins; D006418:Heme / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011233:Precipitin Tests; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012706:Serratia marcescens / Q000235:genetics / Q000378:metabolism*; D012808:Sigma Factor; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D002352:Carrier Proteins; C093956:HasA protein, Serratia marcescens; C116165:HasR protein, Streptomyces ambofaciens; D006454:Hemoglobins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D012808:Sigma Factor; C057616:tonB protein, Bacteria; D006418:Heme