Quantification of cellular properties from external fields and resulting induced velocity: cellular hydrodynamic diameter.
An experimental technique is discussed in which the size distribution of a population of cells is determined by calculating each cell's settling velocity. The settling velocity is determined from microscopically obtained images which were recorded on SVHS tape. These images are then computer imaged and processed, and the cell's location and velocity are determined using a computer algorithm referred to as cell tracking velocimetry (CTV). Experimental data is presented comparing the distribution of human lymphocytes and a human breast cancer cell line, MCF-7, determined using a Coulter counter and the CTV approach.
Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA. Chalmers.1@osu.edu
J J
JJ
Chalmers
S
S
Haam
Y
Y
Zhao
K
K
McCloskey
L
L
Moore
M
M
Zborowski
P S
PS
Williams
D000465:Algorithms; D002478:Cells, Cultured; D003198:Computer Simulation; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018189:Immunomagnetic Separation; D008214:Lymphocytes / Q000166:cytology; D014407:Tumor Cells, Cultured