Expression of CYP2E1 by human monocyte-derived macrophages.
The expression of the ethanol-metabolizing cytochrome P450 (CYP2E1) in human monocyte-derived macrophages was studied at the mRNA and protein levels. The presence of mRNA was investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) and protein by immunocytochemistry. The data show that CYP2E1 is expressed in human monocyte-derived macrophages at a level similar to that demonstrated in other extrahepatic tissues. Although there is circumstantial evidence for the presence of CYP2E1 in human macrophages, it has not previously been demonstrated directly. Its presence in macrophages underlines the potential importance of these cells in initiating alcohol-induced cytotoxicity.
Department of Haematology, Imperial College School of Medicine, St Mary's Hospital, London W2 1PG, U.K.
J L
JL
Hutson
S N
SN
Wickramasinghe
D019392:Cytochrome P-450 CYP2E1 / Q000032:analysis* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000201:enzymology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1