Flavonoids from Cleome droserifolia suppress NO production in activated macrophages in vitro.
The effect of an Egyptian medicinal plant, Cleome droserifolia (Forssk.) Del. on nitric oxide (NO) production in bacillus Calmette-Guérin-induced mouse peritoneal macrophages activated by lipopolysaccharide was investigated in vitro. The methanol extract of C. droserifolia reduced the NO production, and two flavonoids were isolated as the active components. The new one was determined to be 5,4'-dihydroxy-6,7,8,3',5'-pentamethoxyflavone (1) and the other was identified as 5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone (8-methoxycirsilineol; 2). Compound 1 concentration-dependently suppressed the NO production and was effective at a non-toxic concentration (12.5 micrograms/ml). The suppressive activity of 2 was weaker than that of 1.
Fushiya
S
S
Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan. fushiya@mail.pharm.tohoku.ac.jp
Kishi
Y
Y
Hattori
K
K
Batkhuu
J
J
Takano
F
F
Singab
A N
AN
Okuyama
T
T
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D009163:Mycobacterium bovis; D009569:Nitric Oxide / Q000096:biosynthesis*; D010946:Plants, Medicinal
D005419:Flavonoids; D009569:Nitric Oxide; D019833:Genistein