Phenotypic variation in Actinobacillus actinomycetemcomitans during laboratory growth: implications for virulence.
This study examined alteration of specific virulence traits associated with phenotypic changes seen when a low-passage disease-associated and well maintained parent strain of Actinobacillus actinomycetemcomitans was compared to a laboratory-grown spontaneous variant/mutant. Clinical isolates of A. actinomycetemcomitans recovered from periodontitis patients typically grow as rough, adherent colonies on primary culture but undergo transformation to smooth, non-adherent colonies following repeated passage in vitro. The relationship of these phenotypic changes to the virulence of the organism or to the processes that underlie this transformation are not understood. A fresh clinical isolate, designated strain CU1000, was obtained from the first molar site of a patient with classical signs of localized juvenile periodontitis and used as the parent strain to study virulence-related phenotypes. Following several passages of CU1000 on selective agar, a spontaneous variant that demonstrated smooth, opaque, non-adherent colonies was isolated and designated strain CU1060. This study compared the properties of these two strains with respect to colony morphology, autoaggregation, surface appendages, adherence to saliva-coated hydroxyapatite (SHA), LPS chemotype and activity, induction of fibroblast proteinase activity and antigenic properties. CU1000 demonstrated rough, raised, star-positive colonies which upon electron microscopic examination revealed the presence of large, flexible, bundled fibrils. In addition, CU1000 showed adherence to SHA, several unique protein antigens and elevated endotoxin and fibroblast proteinase activity. CU1060, on the other hand, showed minimal adherence to SHA and fewer reactive proteins compared to the fresh clinical isolates. This strain formed smooth, opaque colonies on agar, showed minimal fibril formation and limited endotoxin and fibroblast-proteinase-inducing activity. These findings demonstrate that clinical isolates of A. actinomycetemcomitans undergo significant virulence-reducing phenotypic alterations during in vitro passage and support the need to study this organism in its clinical form.
Fine
Daniel H
DH
Department of Oral Pathology and Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
Furgang
David
D
Department of Oral Pathology and Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
Schreiner
Helen C
HC
Department of Oral Pathology and Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
Goncharoff
Paul
P
Department of Oral Pathology and Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
Charlesworth
Jon
J
Electron Microscopy Core Facility, Mayo Clinic, Rochester, Minnesota, USA.
Ghazwan
Ghazi
G
Department of Periodontlogy, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
Fitzgerald-Bocarsly
Patricia
P
Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
Figurski
David H
DH
Department of Journal of General Microbiology, College of Physicians & Surgeons, Columbia University, New York, NY, USA.
D000189:Actinobacillus Infections / Q000378:metabolism; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D017364:Collagenases / Q000378:metabolism; D005347:Fibroblasts / Q000201:enzymology; D006737:Horseshoe Crabs / Q000166:cytology; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D014774:Virulence
D008070:Lipopolysaccharides; D010455:Peptides; D017364:Collagenases