Ontogeny and species differences in the pancreatic expression and localization of the CCK(A) receptors.
We have evaluated the presence and localization of the CCK(A) receptor in rat, mouse, pig and human fetal pancreas by Northern, Western blots and immunofluorescence techniques. In the rat, parallelism exists between development of the CCK(A) receptor mRNA and protein with maximal peaks of expression during the suckling period. In the course of pancreatitis induction, CCK(A) receptor mRNA were maximally expressed and sustained during the gland's regeneration. In the rat and mouse pancreas, the CCK(A) receptor protein is localized around the acinar cells and beta cells of the islets of Langerhans. In the adult pig and fetal human pancreas, the CCK(A) receptor proteins were detected by Western blot. By immunofluorescence, its detection was possible only in the islet of Langerhans of the pig pancreas. These new findings support the views that CCK plays important and various roles in specific physiological systems of the pancreas of different species.
Département de médecine, Faculté de médecine, Sherbrooke, Quebec, J1H 5N4, Canada.
J
J
Bourassa
J
J
Lainé
M L
ML
Kruse
M C
MC
Gagnon
E
E
Calvo
J
J
Morisset
D000818:Animals; D002766:Cholecystokinin / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051379:Mice; D010179:Pancreas / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin / Q000819:agonists / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012038:Regeneration; D013045:Species Specificity; D013552:Swine
D012333:RNA, Messenger; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin