Direct determination of methamphetamine enantiomers in urine by liquid chromatography with a strong cation-exchange precolumn and phenyl-beta-cyclodextrin-bonded semi-microcolumn.
Methamphetamine enantiomers in drug abusers' urine were clearly separated by semi-microcolumn liquid chromatography using a column-switching system. The system consists of two separation processes: firstly, a strong cation-exchange precolumn removes neutral and anionic substances in urine, and then methamphetamine enantiomers trapped in the column are transferred to and separated in a phenyl-beta-cyclodextrin-bonded semi-microcolumn (Chiral Drug, 150 mm x 1.5 mm I.D.). (S)-(+)-Methamphetamine was baseline separated from (R)-(-)-methamphetamine within 25 min, directly from urine samples. The detection limit for both enantiomers was 0.1 microg/ml.
Makino
Y
Y
Kanto-Shin'etu Regional Narcotic Control Office, Ministry of Health and Welfare, Tokyo, Japan.
Suzuki
A
A
Ogawa
T
T
Shirota
O
O
D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003505:Cyclodextrins; D006801:Humans; D008694:Methamphetamine / Q000652:urine*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D002411:Cation Exchange Resins; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D008694:Methamphetamine; C031215:betadex