Chromosome band 16q24 is frequently deleted in human gastric cancer.
We have analysed the loss of heterozygosity (LOH) on chromosome bands 16q22-q24 in 24 primary gastric cancer tissues and found three regions of frequent allelic loss (16q22, 16q24.1-q24.3 and 16q24.3). The region for the most frequent allelic loss (63%) was in 16q24.1-q24.3. LOH of this region had no relationship with histological subtype, but a significant association between LOH and microscopic lymphangial invasion was observed. Although not significant, vascular and gastric wall invasions are also associated with LOH. The region includes the locus for the H-cadherin gene. Therefore we examined the genetic and epigenetic alterations of this gene. Markedly reduced expression was observed in gastric cancer cell lines compared with that of normal gastric mucosa. However, no mutation was found in this gene in any of the gastric cancer tissues or the gastric cancer cell lines. Furthermore, we analysed the methylation status of the 5'-flanking region of the gene, but no significant association was found. We suggest that some other tumour suppressor gene(s) in 16q24.1-q24.3 may be responsible for gastric carcinogenesis.
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
Y
Y
Mori
M
M
Matsunaga
T
T
Abe
S
S
Fukushige
K
K
Miura
M
M
Sunamura
K
K
Shiiba
M
M
Sato
T
T
Nukiwa
A
A
Horii
D000328:Adult; D000368:Aged; D000483:Alleles; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D002872:Chromosome Deletion; D002885:Chromosomes, Human, Pair 16; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D015820:Cadherins; C099972:H-cadherin