Red blood cell membrane integrity in primary open angle glaucoma: ex vivo and in vitro studies.
OBJECTIVE
There is increasing evidence that abnormal perfusion of the optic nerve head is an important factor involved in the pathophysiology of glaucoma. Transport and distribution of oxygen to the tissues takes place through the erythrocyte membrane. Red blood cell (RBC) acetylcholinesterase (AChE) is a marker of RBC membrane integrity. The aim of this study was to find out whether RBC membrane integrity is preserved in primary open angle glaucoma (POAG), and whether it is modified by the use of topical timolol maleate and pilocarpine.


METHODS
RBC AChE activity was determined ex vivo by Kaplan's spectrophotometric method in 19 POAG patients undergoing topical treatment for glaucoma with timolol, pilocarpine or a combination of the two drugs, and compared with that in 20 controls. To assess the effect of antiglaucomatous therapy in our findings, we carried out an in vitro study in 26 non-glaucomatous patients in which we measured RBC AChE activity after incubation of blood with either timolol maleate, pilocarpine or a combination of the two drugs, using the same spectrophotometric method.


RESULTS
There was a significant increase in RBC AChE enzyme activity in POAG patients compared with the control group (p < 0.002). However, timolol and pilocarpine, individually or in combination, have the opposite effect, significantly decreasing RBC AChE activity (p < 0.05).


CONCLUSIONS
This change in RBC AChE enzyme activity could suggest alterations in RBC membrane integrity in primary open angle glaucoma. Whether or not this finding has implications regarding the microcirculation at the optic nerve head needs to be investigated further.
Zabala
L
L
Centro de Estudos de Microcirculação e Biopatologia Vascular, Faculdade de Medicina de Lisboa, Portugal.
Saldanha
C
C
Martins e Silva
J
J
Souza-Ramalho
P
P
D000110:Acetylcholinesterase / Q000097:blood; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000368:Aged; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D018721:Muscarinic Agonists / Q000494:pharmacology; D010277:Parasympathomimetics / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D013999:Timolol / Q000494:pharmacology
D000319:Adrenergic beta-Antagonists; D018721:Muscarinic Agonists; D010277:Parasympathomimetics; D010862:Pilocarpine; D013999:Timolol; D000110:Acetylcholinesterase