Identification of rubella virus T-cell epitopes recognized in anamnestic response to RA27/3 vaccine: associations with boost in neutralizing antibody titer.
Rubella virus (RV)-specific cell-mediated immunity (CMI) and antibodies were measured in healthy adolescents reimmunized with measles-mumps-rubella (MMR) vaccine. Lymphocyte proliferation to RV synthetic peptides was determined before and at 2, 4 and 10 weeks after, MMR. After MMR, increased CMI was observed with 16 peptides, including six containing antibody neutralization (NT) domains. Positive CMI (stimulation index > or =2.0) to E1(254-285) and C(1-29) before vaccination was significantly associated with a boost in NT titers, while positive CMI at weeks 2 or 4 to E1(254-285), E1(301-314), E1(389-408), E1(462-481), E2(134-150), E2(140-156), E2(168-179), C(1-29) and C(88-111) showed the same association.
Mitchell
L A
LA
Department of Pathology, University of British Columbia, Vancouver, Canada. leslie@wpog.childhosp.bc.ca
Tingle
A J
AJ
Décarie
D
D
Shukin
R
R
D000293:Adolescent; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D018984:Epitopes, T-Lymphocyte / Q000032:analysis* / Q000276:immunology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007111:Immunity, Cellular; D007156:Immunologic Memory; D016131:Lymphocyte Subsets / Q000276:immunology / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology; D012409:Rubella / Q000276:immunology / Q000517:prevention & control*; D012411:Rubella Vaccine / Q000276:immunology*; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology
D000914:Antibodies, Viral; D018984:Epitopes, T-Lymphocyte; D010446:Peptide Fragments; D012411:Rubella Vaccine; D014613:Vaccines, Attenuated