Serum starvation induced secondary V lambda J lambda rearrangement in a human plasma B cell line.
HB4C5 is a human antibody producing plasma B cell line that expresses the recombination activating gene-1 (RAG-1) and RAG-2 constitutively, but undergoes few secondary immunoglobulin gene rearrangements when cultured in fetal bovine serum-containing medium. Here, we found that depletion of serum from the culture media induces secondary VlambdaJlambda rearrangement in this cell line. To investigate the induction mechanism of secondary VlambdaJlambda rearrangement, we assessed the expression levels of RAG-1 and RAG-2 products, Vlambda germline transcription level and the amount of Vlambda signal broken ends (SBE) in HB4C5 cells cultured in serum-supplemented or serum-free medium. Western-blot analysis showed that the expression level for the RAG-1 and RAG-2 proteins was not affected by the serum depletion. Vlambda germline transcript was found to be constitutively expressed in HB4C5 cell line and this transcription level was not affected by the lack of serum. On the other hand, the amount of Vlambda SBE was shown to be increased in HB4C5 cells cultured in serum-free medium, suggesting that this increased formation of Vlambda SBE at least partly contributed to the enhanced occurrence of secondary VlambdaJlambda rearrangement in HB4C5 cells cultured in the serum-free condition. These results indicate that expression of RAG proteins and Vlambda germline transcription is not enough to undergo secondary VlambdaJlambda rearrangement in this cell line.
Graduate School of Bioresources and Bioenvironmental Science, Kyushu University, Fukuoka, Japan.
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Haruta
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Tachibana
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Yamada
D002051:Burkitt Lymphoma; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000276:immunology; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain / Q000187:drug effects; D016654:Genes, RAG-1 / Q000276:immunology; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism*; D014158:Transcription, Genetic / Q000276:immunology; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D018398:Homeodomain Proteins; D007135:Immunoglobulin Variable Region; C064658:RAG-1 protein