Neurogenic modifications induced by substance P in an organ culture of human skin.
Neurogenic inflammation of the skin observed after topical application of an irritant substance or environmental stimulation induces vascular changes and the production of inflammatory mediators. Substance P (SP) is one of the main neuropeptides which trigger an inflammatory response in the skin. So, with the aim to develop an alternative method to study neurogenic inflammation of the skin, we used an organ culture of human skin. SP was added onto epidermis or directly to culture medium in an attempt to reproduce ex vivo the effects described in vivo. Even disconnected from systemic blood circulation, in skin fragments in culture, we observed dose-dependent edema, vasodilation and extravasation of lymphocytes and mast cells through the microvascular wall. Moreover, the release of proinflammatory mediators interleukin 1alpha and tumor necrosis factor alpha was evidenced.
Service Central d'Anatomie et Cytologie Pathologiques, Hôpital Pitié-Salpétrière, Paris, France.
M C
MC
Branchet-Gumila
S
S
Boisnic
Y
Y
Le Charpentier
I
I
Nonotte
C
C
Montastier
L
L
Breton
D000328:Adult; D003470:Culture Media; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects; D008875:Middle Aged; D009924:Organ Culture Techniques; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000294:innervation*; D013373:Substance P / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects
D003470:Culture Media; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P