Dynamic expression pattern of Ca(2+)/calmodulin-dependent protein kinase II gene in the central nervous system of Drosophila throughout development.
Calcium/calmodulin-dependent protein kinase II (CaM KII) is thought to be involved in the majority of the neuronal functions mediated by intracellular free Ca(2+), and has been implicated in long-term potentiation, learning, and memory. In this work, we have examined in detail the RNA expression pattern for the Drosophila CaM KII gene by in situ hybridization, during embryonic, larval, pupal, and adult stages. Our results indicate that expression of CaM KII was homogeneous in early embryos, but that during development the gene transcription rapidly became restricted to neuroblasts and their progeny in the nervous system. This predominant expression in the nervous system is maintained during late embryogenesis and post-embryonic development. A signal compartmentalization appeared in the larval central nervous system, where the CaM KII expression became progressively concentrated in the anterior ganglia. In the adult brain, a specific expression was more abundant in a subset of neurons around the central brain, particularly the mushroom bodies and the central complex, structures that play an important role in learning and memory.
Rachidi
M
M
Department of Molecular Biology, Pasteur Institute, 25 rue du Docteur Roux, Paris, 75015, France.
Lopes
C
C
Takamatsu
Y
Y
Ohsako
S
S
Benichou
J C
JC
Delabar
J M
JM
D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D019070:Cell Lineage; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000201:enzymology; D047108:Embryonic Development; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D017403:In Situ Hybridization; D007814:Larva / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D009473:Neuronal Plasticity; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D011679:Pupa / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D012333:RNA, Messenger; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases