Steroid-DNA conjugates: improved triplex formation with 5-amido-(7-deoxycholic acid)-dU incorporated oligonucleotides.
The synthesis of oligodeoxyribonucleotides incorporating 7-deoxycholic acid conjugated at C5 of dU is described. When used as third strand, these form oligonucleotide triplexes with higher stability compared to unmodified controls at physiological pH.
Division of Organic Chemistry (Synthesis), National Chemical Laboratory, Pune, India.
D
D
Bhatia
L
L
Yue-Ming
K N
KN
Ganesh
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000528:radiation effects; D013256:Steroids / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D014466:Ultraviolet Rays; D014498:Uracil / Q000737:chemistry
D009841:Oligonucleotides; D013256:Steroids; D014498:Uracil; D004247:DNA