13q14 deletion in non-Hodgkin's lymphoma: correlation with clinicopathologic features.
OBJECTIVE
13q14 deletion frequently occurs as a single anomaly in chronic lymphocytic leukemia (CLL) with favorable prognosis. This study was performed to assess the distribution of 13q14 deletion in non-Hodgkin's lymphoma (NHL) and to analyze its correlation with salient clinicopathologic features.


METHODS
One hundred and twenty-five NHL were analyzed by cytogenetics and by interphase fluorescence in situ hybridization (FISH), using a 13q14 cosmid probe recognizing DNA sequences between the Rb gene and the D13S25 marker. Clinical records all patients were surveyed.


RESULTS
A 13q14 rearrangement was present in the stemline in 10 patients; 15 additional cases were shown by FISH to carry 13q14 deletion in 55-90% of the interphase cells, giving a 20% overall incidence for this anomaly. Six of 44 patients had a low-grade NHL, 14/28 had mantle cell lymphoma (MCL), 5/42 had a high grade NHL (p<0.0001). There was not correlation between 13q, karyotype status and complexity. A statistically significant association was found between 13q-, presence of splenomegaly and PB involvement, lower probability of attaining complete remission (CR) and shorter survival. These findings were not simply a function of the association of 13q- with MCL. In multivariate analysis, a complex karyotype had prognostic importance (p=0.0078), along with age (p=0.01), histology (p=0.001), LDH (p=0.03), PS (p=0.001), sex (p=0.03) and splenomegaly (p=0.02).


CONCLUSIONS
13q14 deletion represented an early chromosome change and showed a preferential association with MCL, though it was found in virtually all principal histologic subtypes, irrespective of clinical stage, karyotype status and complexity. Patients with 13q14 deletions had a low CR rate, suggesting that genes relevant to lymphomagenesis are located in this chromosome segment that warrants molecular cytogenetic investigation.
Cuneo
A
A
Istituto di Ematologia, Università di Ferrara, via Savonarola 9, 44100 Ferrara, Italy. sse@dns.unife.it
Bigoni
R
R
Rigolin
G M
GM
Roberti
M G
MG
Bardi
A
A
Campioni
D
D
Minotto
C
C
Agostini
P
P
Milani
R
R
Bullrich
F
F
Negrini
M
M
Croce
C
C
Castoldi
G
G
D014408:Biomarkers, Tumor; D002882:Chromosomes, Human, Pair 13; D015321:Gene Rearrangement; D016161:Genes, Retinoblastoma; D006801:Humans; D007621:Karyotyping; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000503:physiopathology; D015999:Multivariate Analysis; D011379:Prognosis; D017384:Sequence Deletion
D014408:Biomarkers, Tumor