Phosphacan immunoreactivity is associated with perineuronal nets around parvalbumin-expressing neurones.
A special feature of the extracellular matrix in adult brains of various species is the concentration of certain components around different sub-populations of neurones, giving rise to net-like structures termed perineuronal nets. Recently, some of these components have been identified but the function of these nets has yet to be resolved. Using immunofluorescence microscopy, we report here that phosphacan, a chondroitin sulphate proteoglycan, is an additional component of Wisteria floribunda labelled perineuronal nets surrounding parvalbumin-expressing neurones in rat cerebral cortex. Glycoproteins such as tenascin-C and -R have been identified in perineuronal nets and the present detection of phosphacan immunoreactivity in the same entity is of potential physiological importance because of their previously described interactions.
Haunso
A
A
Institute of Histology and General Embryology and Program in Neuroscience, University of Fribourg, CH-1705, Fribourg, Switzerland.
Celio
M R
MR
Margolis
R K
RK
Menoud
P A
PA
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D037102:Lectins; D008297:Male; D009415:Nerve Net / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D034781:Receptors, N-Acetylglucosamine
D011508:Chondroitin Sulfate Proteoglycans; D037102:Lectins; D010320:Parvalbumins; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin; C516897:Ptprz1 protein, rat; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5