Sequencing of argentinated peptides by means of electrospray tandem mass spectrometry.
A strategy for semiautomatic sequencing of argentinated (silver-containing) oligopeptides has been developed. Sequencing is based on a search algorithm that identifies a triplet peak relationship in a product ion spectrum of the [M + Ag]+ ion of an oligopeptide. The ions that constitute a triplet are [bn + OH + Ag]+, [bn - H + Ag]+, and [a(n) - H + Ag]+, which are separated by 18 and 28 m/z units, respectively. The difference in the m/z values of adjacent triplets identifies the residue that is "cleaved". Observation of the [yn + H + Ag]+ ion containing the cleaved residue confirms the assignment. Sequencing of argentinated tryptic peptides may prove useful for automated proteome analysis via the sequence tag method.
Chu
I K
IK
Department of Chemistry, York University, Toronto, Ontario, Canada.
Guo
X
X
Lau
T C
TC
Siu
K W
KW
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D014357:Trypsin
D009842:Oligopeptides; D012834:Silver; D014357:Trypsin