Beta(1-40) amyloid peptide injection into the nucleus basalis of rats induces microglia reaction and enhances cortical gamma-aminobutyric acid release in vivo.
The nucleus basalis of adult rats was injected with beta(1-40) amyloid peptide. A marked increase in basal and K(+)-evoked GABA release in the ipsilateral cortex and a significant decrease in GAD activity in the injected NB were found 30 days after injection. An intense activation of microglial cells that surrounded and infiltrated the deposit was observed. These data demonstrate that a local injection of beta(1-40) peptide into the NB induces glia activation and affects GABAergic neurons.
Department of Pre-Clinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy.
C
C
Scali
C
C
Prosperi
L
L
Giovannelli
L
L
Bianchi
G
G
Pepeu
F
F
Casamenti
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D008297:Male; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D008845:Microinjections; D009498:Neurotoxins / Q000494:pharmacology*; D010296:Parietal Lobe / Q000187:drug effects* / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013377:Substantia Innominata / Q000187:drug effects* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D009498:Neurotoxins; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D005680:gamma-Aminobutyric Acid