Paracoccidioides brasiliensis. A mycologic and immunochemical study of two strains.
The authors conducted a mycologic, immunochemical and molecular biology study on two strains of Paracoccidioides brasiliensis, one of them, called IBIA, isolated from soil in the municipality of IBIA (Minas Gerais) by Silva-Vergara et al. (1996, 1998), and the other, BAT, cultivated from a human case of paracoccidioidomycosis in Ribeirão Preto (São Paulo/Brazil) by Freitas Da Silva (1996). Both strains showed cotton-like (M) and yeast-like (Y) forms and were pathogenic for testicularly inoculated guinea pigs, producing granulomatous and/or suppurative orchitis. Immunochemically was demonstrated the presence of gp43 by double immunodiffusion, immunoelectrophoresis and immunoblotting.
Laboratório de Micologia Médica do Instituto de Medicina Tropical de São Paulo/LIM 53 HCFMUSP, Brasil. valacaz@usp.com.br
C da S
Cda S
Lacaz
M S
MS
Vidal
E M
EM
Heins-Vaccari
N T
NT
de Melo
G M
GM
Del Negro
G L
GL
Arriagada
R dos S
Rdos S
Freitas
D000818:Animals; D006168:Guinea Pigs; D006801:Humans; D007120:Immunochemistry / Q000379:methods; D016533:Mycological Typing Techniques; D010228:Paracoccidioides / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification