Urine as another potential source for template DNA in polymerase chain reaction (PCR).
This technical note examines the potential for preparing template DNA in polymerase chain reactions (PCR) from urine in Japanese macaques (Macaca fuscata). Microsatellite band patterns from urine samples showed close agreement with those of blood and fecal samples, and only a few hundred microl of urine yielded a template DNA for PCR. This research will increase the opportunity for scientists to examine the genetic backgrounds of their target animals by using non-invasive sample collection in the wild.
Department of Ecology and Social Behavior, Primate Research Institute, Kyoto University, Aichi-ken, Japan.
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Hayakawa
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Takenaka
D000818:Animals; D000830:Animals, Laboratory; D000835:Animals, Wild; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000652:urine*; D005243:Feces / Q000737:chemistry; D007564:Japan; D008251:Macaca / Q000235:genetics* / Q000652:urine; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013698:Templates, Genetic
D004247:DNA