A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation.
D-type cyclins are believed to regulate the onset of cell division upon mitogenic signaling. Here, the isolation is reported of a new D-type cyclin gene (CYCD4;1) of Arabidopsis thaliana (L.) Heynh. during a two-hybrid screen using the cyclin-dependent kinase CDC2aAt as bait. Transcription of CYCD4;1 can be induced by sucrose. The co-regulated expression of CYCD4;1 and CDC2aAt in starved suspension cultures upon mitogenic stimulation indicates that the formation of a complex between these two partners is important for the resumption of cell division activity. By in-situ hybridizations CYCD4;1 was shown to be expressed during vascular tissue development, embryogenesis, and formation of lateral root primordia. Expression during the latter process suggests that the induced expression of D-type cyclins by mitogenic stimuli might be one of the rate-limiting events for the initiation of lateral roots.
De Veylder
L
L
Department of Plant Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, Belgium.
de Almeida Engler
J
J
Burssens
S
S
Manevski
A
A
Lescure
B
B
Van Montagu
M
M
Engler
G
G
Inzé
D
D
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D001483:Base Sequence; D056741:Cyclin D; D016213:Cyclins / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D056741:Cyclin D; D016213:Cyclins; D018076:DNA, Complementary