Dichlorophenyl phosphoramidates as substrates for avian and mammalian liver phosphotriesterases: activity levels, calcium dependence and stereospecificity.
The present study shows the existence of both Ca2+-dependent and EDTA-resistant hydrolysing activities against HDCP and paraoxon in the particulate and soluble fractions of hen, rat and rabbit liver. HDCP was more extensively hydrolysed than paraoxon in both subcellular fractions and each of three individuals of the three animal species under study in spite of wide interindividual variations. However the ratio of HDCP versus paraoxon hydrolysing activity (HDCPase/paraoxonase), although within the same order of magnitude, cannot be considered as constant as it ranges one- to seven-fold between individuals of the same species. Also there is no constant ratio of Ca2+-dependent/EDTA-resistant activities. Rabbit liver showed the highest rates of Ca2+-dependent hydrolysis for both organophosphorus compounds whereas the hen paraoxonase activity was not inhibited by EDTA. The stereospecific hydrolysis of HDCP was mostly a Ca2+-dependent one, the S-HDCP isomer being hydrolysed faster than the R-HDCP one. The suggestion is made that HDCP could be conveniently used to measure PTE activity in the liver.
Instituto de Neurociencias, Universidad Miguel Hernández, San Juan de Alicante, Alicante, Spain.
A
A
Monroy-Noyola
M A
MA
Sogorb
N
N
Díaz-Alejo
N
N
Níguez
J
J
Barril
J L
JL
Vicedo
M A
MA
Escudero
E
E
Vilanova
D000818:Animals; D043303:Aryldialkylphosphatase; D002118:Calcium / Q000502:physiology*; D002645:Chickens; D002800:Cholinesterase Inhibitors / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004950:Esterases / Q000378:metabolism*; D005260:Female; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D013379:Substrate Specificity
D002800:Cholinesterase Inhibitors; D009943:Organophosphorus Compounds; C066606:hexyl 2,5-dichlorophenylphosphoroamidate; D004492:Edetic Acid; D004950:Esterases; D043303:Aryldialkylphosphatase; D002118:Calcium