Immunization of RANTES expression plasmid with a DNA vaccine enhances HIV-1-specific immunity.
Cytokines play important roles in regulating immune response. This study evaluated the adjuvant effect of an expression plasmid encoding RANTES (regulated on activation normal T-cell expressed and secreted) chemokine on the immunity induced by a DNA vaccine. This vaccine consists of expression plasmids encoding the env and rev genes of human immunodeficiency virus type 1 (HIV-1). DNA vaccination with RANTES plasmid induced significantly higher titers of serum HIV-1-specific IgG and IgG2a antibodies than DNA vaccination alone on both intramuscular and intranasal immunization. This combination also increased HIV-1-specific cytotoxic T lymphocyte activity and delayed-type hypersensitivity. Intranasal immunization induced a higher titer of fecal secretory IgA antibody than intramuscular immunization. These results demonstrate that coadministration of RANTES plasmid dominantly induced HIV-1-specific cell-mediated immunity.
Department of Bacteriology, Yokohama City University School of Medicine, Yokohama, 236, Japan.
K Q
KQ
Xin
Y
Y
Lu
K
K
Hamajima
J
J
Fukushima
J
J
Yang
K
K
Inamura
K
K
Okuda
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D018946:Chemokine CCL5 / Q000276:immunology*; D005260:Female; D015497:HIV-1 / Q000276:immunology*; D006644:Histiocytes / Q000737:chemistry / Q000166:cytology; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000473:pathology / Q000821:virology; D007111:Immunity, Cellular / Q000276:immunology; D008214:Lymphocytes / Q000737:chemistry / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000166:cytology; D019444:Vaccines, DNA / Q000737:chemistry* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D018946:Chemokine CCL5; D019444:Vaccines, DNA