Acetylcholinesterase-positive innervation is present at undifferentiated stages of the sea turtle Lepidochelis olivacea embryo gonads: implications for temperature-dependent sex determination.
In embryos of different reptile species, incubation temperature triggers a cascade of endocrine events that lead to gonad sex differentiation. The cellular and molecular mechanisms by which temperature sets in motion this process are still controversial. Here, we begin evaluating the possible participation of the nervous system in temperature-dependent sex determination by showing the existence and origin of acetylcholinesterase (AchE)-positive nerve fibers in undifferentiated gonads of the Lepidochelys olivacea (L. olivacea) sea turtle putative male and female embryos, along the thermosensitive period for sex determination (TPSD; stages 20-27). AChE-positive nerve bundles and fibers were readily visualized until developmental stage 24 and thereafter. DiI injections and confocal imaging showed that some of these gonadal nerves arise from the lower thoracic and upper lumbar spinal cord levels, and might thus be sensory in nature. Because the vertebrate spinal cord is capable of integrating by itself thermoregulatory responses with no intervention of uppermost levels of the central nervous system, we also evaluated spinal cord maturation during the TPSD. The maturation of the spinal cord was more advanced in putative female than in male embryos, when sex determination is taking place for each sex; this process starts and ends earlier in male than in female embryos. Together these observations open the possibility that the spinal cord and the innervation derived from it could play a direct role in driving or modulating the process of temperature-dependent gonad sex determination and/or differentiation, particularly in female L. olivacea embryos.
Gutiérrez-Ospina
G
G
Department of Development Neurobiology, Center of Neurobiology, National University of México, Campus UNAM-UAQ-Juriquilla, Querétaro, México.
Jiménez-Trejo
F J
FJ
Favila
R
R
Moreno-Mendoza
N A
NA
Granados Rojas
L
L
Barrios
F A
FA
Díaz-Cintra
S
S
Merchant-Larios
H
H
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000502:physiology; D005260:Female; D006066:Gonads / Q000196:embryology* / Q000294:innervation; D008297:Male; D009420:Nervous System / Q000196:embryology*; D012733:Sex Differentiation / Q000502:physiology; D013116:Spinal Cord / Q000196:embryology; D013696:Temperature; D014426:Turtles / Q000196:embryology*
D000110:Acetylcholinesterase