Medium optimization for spore production of coniothyrium minitans using statistically-based experimental designs
Statistically-based experimental designs were used to optimize a chemically defined solid medium for the spore production of Coniothyrium minitans. In the first optimization step the influence of starch, urea, phosphate, magnesium, calcium, thiamin and trace elements on spore production was evaluated using a fractional factorial design. Starch and trace elements influenced spore production positively while urea affected spore production negatively. The other components had no significant influence on spore production. In the second and third steps the concentrations of starch, urea and trace elements were further optimized using central composite designs and response surface analysis. This optimization strategy allowed the spore production to be increased by a factor 7 from 4 x 10(9) to almost 3 x 10(10) spores per Petri dish of 9 cm diameter. Copyright 1999 John Wiley & Sons, Inc.
Agrotechnological Research Institute (ATO-DLO), Industrial Agrobiotechnology, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
LP
Ooijkaas
EC
Wilkinson
J
Tramper
RM
Buitelaar