Linoleic acid hydroperoxide favours hypochlorite- and myeloperoxidase-induced lipid peroxidation.
Liposomes composed of soybean phosphatidylcholine were peroxidized using the reagent sodium hypochlorite or the myeloperoxidase-hydrogen peroxide-Cl- system. Linoleic acid hydroperoxide previously prepared from linoleic acid by means of lipoxidase was incorporated into liposomes. The yield of thiobarbituric acid reactive substances (TBARS) continuously increased with higher amounts of hydroperoxide groups after the initiation of lipid peroxidation by hypochlorous acid producing systems. The accumulation of TBARS was inhibited by scavengers of free radicals such as butylated hydroxytoluene and by the scavengers of hypochlorous acid, taurine and methionine. Lipid peroxidation was also prevented by sodium azide or chloride free medium in the myeloperoxidase-hydrogen peroxide-Cl- system. Here we show for the first time that the reaction of hypochlorous acid with a biologically relevant hydroperoxide yields free radicals able to cause further oxidation of lipid molecules.
Research Institute of Physico-Chemical Medicine, Moscow, Russia.
O M
OM
Panasenko
J
J
Arnhold
D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006997:Hypochlorous Acid / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism*; D008081:Liposomes / Q000187:drug effects / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D008715:Methionine / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D019810:Sodium Azide / Q000494:pharmacology; D013025:Glycine max; D013654:Taurine / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D002712:Chlorides; D016166:Free Radical Scavengers; D005609:Free Radicals; D008041:Linoleic Acids; D008054:Lipid Peroxides; D008081:Liposomes; D010713:Phosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; D013654:Taurine; D002084:Butylated Hydroxytoluene; C008285:linoleic acid hydroperoxide; D008353:Mannitol; D006997:Hypochlorous Acid; D019810:Sodium Azide; D008715:Methionine; D006861:Hydrogen Peroxide; D009195:Peroxidase