Hydroperoxidase activity of lipoxygenase in the presence of cyclodextrins.
The oxidation of xenobiotics by the hydroperoxidase activity of lipoxygenase in the presence of cyclodextrins was studied. These produced an inhibitory effect on xenobiotics oxidation, based on their degree of hydrophobicity and the charge (isoproterenol < 4-methyl-catechol (4MC) < 4-tert-butylcatechol (TBC) < 4-tert-octylcatechol (TOC)). This inhibitory effect was due to the complexation of xenobiotics in the hydrophobic cavity of cyclodextrins. The complexation constant Kc was calculated by nonlinear regression of the inhibition curves obtained in the presence of cyclodextrins, and the values obtained were 400, 16,250, and 35,127 M-1 for 4MC, TBC, and TOC, respectively. The validity of these values was checked at different points of the Michaelis-Menten saturation curve, and a sigmoidal inhibition curve was obtained at the saturating concentration of the o-diphenol, TBC, with no change in the Kc value. This demonstrates the validity of the equations used to calculate Kc for the complete range of the Michaelis-Menten equation.
Núñez-Delicado
E
E
Faculty of Biology, University of Murcia, Murcia, E-30071, Spain.
Sojo
M M
MM
Sánchez-Ferrer
A
A
García-Carmona
F
F
D002396:Catechols / Q000378:metabolism; D003505:Cyclodextrins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D008084:Lipoxygenase / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*; D013053:Spectrophotometry; D015262:Xenobiotics / Q000378:metabolism
C120504:4-tert-octylcatechol; D002396:Catechols; D003505:Cyclodextrins; D015262:Xenobiotics; C018599:4-methylcatechol; D006861:Hydrogen Peroxide; D009195:Peroxidase; D008084:Lipoxygenase