Recombinant human albumin as a stabilizer for biological materials and for the preparation of international reference reagents.
Recombinant human albumin expressed in Saccharomyces cerevisiae was compared with native human serum albumin in its physicochemical properties and in its use as a stabilizer in lyophilized preparations of thyroid-stimulating hormone (TSH), interleukin 15 (IL-15) and granulocyte colony-stimulating factor (G-CSF). Advantages of recombinant albumin include its lack of potential human contaminants and infectious agents. When used at concentrations of 0.1-0.2% (w/v), recombinant albumin was equivalent to native serum albumin in its capacity to protect immunological, biological and biochemical properties of TSH, IL-15 and G-CSF. Physicochemical characteristics of the two forms of albumin including their binding to fatty acids were also similar. The recombinant form of albumin used in this study should be considered as a suitable stabilizer in the preparation of lyophilized products and reference reagents.
Division of Chemistry, National Institute for Biological Standards and Control, Potters Bar, Herts, U.K.
E
E
Tarelli
A
A
Mire-Sluis
H A
HA
Tivnann
B
B
Bolgiano
D T
DT
Crane
C
C
Gee
X
X
Lemercinier
M L
ML
Athayde
N
N
Sutcliffe
P H
PH
Corran
B
B
Rafferty
D004731:Endotoxins; D005227:Fatty Acids / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000592:standards; D006801:Humans; D007202:Indicators and Reagents / Q000592:standards*; D019409:Interleukin-15 / Q000592:standards; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012015:Reference Standards; D012709:Serum Albumin / Q000737:chemistry; D013696:Temperature; D013972:Thyrotropin / Q000592:standards
D004731:Endotoxins; D005227:Fatty Acids; D007202:Indicators and Reagents; D019409:Interleukin-15; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin; D016179:Granulocyte Colony-Stimulating Factor; D013972:Thyrotropin