Fibrinogen adsorbs from aqueous media to microscopic droplets of poly(dimethylsiloxane) and remains coagulable.
Fibrinogen binds from aqueous media containing it to droplets of linear trimethylsilyl-terminated poly(dimethylsiloxane) (PDMS) dispersed in those same media. Once bound, fibrinogen elutes from emulsified droplets of PDMS only very slowly, even when incubated in buffer that contains a physiologic concentration of the protein. The bound fibrinogen is coagulable, as indicated by the thrombin-dependent agglutination of droplets. Thus fibrinogen bound to droplets of PDMS renders an adhesive potential to the surface of the droplets, a potential that may have relevance to the biologic processing of the polymer in vivo.
Whitlock
P W
PW
Department of Materials Science and Engineering and the Polymer Research Center, University of Cincinnati, Cincinnati, Ohio 45221-0012, USA.
Clarson
S J
SJ
Retzinger
G S
GS
D000268:Adhesiveness; D000327:Adsorption; D000371:Agglutination; D001777:Blood Coagulation / Q000502:physiology*; D001798:Blood Proteins / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004655:Emulsions; D005340:Fibrinogen / Q000737:chemistry*; D007202:Indicators and Reagents; D010316:Particle Size; D012828:Silicones / Q000737:chemistry*; D014867:Water
D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D004655:Emulsions; D007202:Indicators and Reagents; D012828:Silicones; D014867:Water; C013830:baysilon; D005340:Fibrinogen