Development and usefulness of new polymerase chain reaction-based tests for detection of different alleles at codons 136 and 171 of the ovine prion protein gene.
OBJECTIVE
To develop new and improved tests to detect alleles at codons 136 and 171 of the ovine prion protein locus and to evaluate the frequency of these alleles.


METHODS
159 Suffolk sheep belonging to 3 flocks.


METHODS
Polymerase chain reaction (PCR) analysis that contained diagnostic restriction site variation for each allele were developed for the relevant gene regions. Alleles were determined by analyzing DNA isolated from buccal swab specimens or blood samples.


RESULTS
At codon 136, frequencies of the alanine and valine alleles were found to be 97 and 3%, respectively. At codon 171, frequencies of the glutamine, arginine, and histidine alleles were found to be 57, 41, and 2%, respectively.


CONCLUSIONS
Little variation was detected in codon 136, whereas noteworthy variation was found in codon 171; > 40% of the alleles at this locus coded for glutamine. Because the glutamine allele at codon 171 confers susceptibility to scrapie, reduction of its frequency is of importance to management of sheep flocks.


CONCLUSIONS
Genotyping of sheep, using the tests reported here, should facilitate selective breeding programs designed to decrease the risk of scrapie.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314, USA.
V
V
Yuzbasiyan-Gurkan
J D
JD
Krehbiel
Y
Y
Cao
P J
PJ
Venta
D000483:Alleles; D000818:Animals; D003062:Codon / Q000235:genetics*; D004247:DNA / Q000097:blood; D017931:DNA Primers / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D009061:Mouth Mucosa / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D017096:Prion Diseases / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D011328:Prions / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000235:genetics*
D003062:Codon; D017931:DNA Primers; D011328:Prions; D004247:DNA; D004262:DNA Restriction Enzymes