High-resolution calorimetric and optical melting profiles of DNA plasmids: resolving contributions from intrinsic melting domains and specifically designed inserts.
We demonstrate that differential scanning calorimetry (DSC) can be used to yield high-resolution melting profiles for DNA plasmids that agree in all major features with the corresponding plasmid melting profiles derived using more traditional optical techniques. We further demonstrate that by combining information derived from both calorimetric and optical melting profiles one can glean insights that are unavailable from either melting curve alone. By using both optical and calorimetric observables, we show how one can resolve, identify, and measure the thermodynamic properties of particular sequences/domains of interest within a plasmid. We also show that complementary DSC and optical melting studies on plasmids with and without specifically designed inserts can provide fundamental advantages over the corresponding melting studies on other model system constructs for thermodynamically characterizing nucleic acid sequences/structures.
Völker
J
J
Department of Chemistry, Rutgers University, 610 Taylor Road, Piscataway, NJ, 08854-8087, USA.
Blake
R D
RD
Delcourt
S G
SG
Breslauer
K J
KJ
D002152:Calorimetry, Differential Scanning; D004247:DNA / Q000737:chemistry; D004926:Escherichia coli; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D013696:Temperature; D013816:Thermodynamics
D004247:DNA