Focal ischemic preconditioning induces rapid tolerance to middle cerebral artery occlusion in mice.
In a process called ischemic preconditioning, a brief, sublethal ischemic insult protects tissue from subsequent, more severe injury. There have been no reports of rapidly induced ischemic preconditioning. The authors sought to develop a model of cerebral ischemic preconditioning in the mouse that can be applied to transgenic and knockout animals. They found that brief middle cerebral artery (MCA) occlusion only minutes before a severe ischemic insult can induce protection from that insult. Here the investigators describe a mouse model of preconditioning using intraluminal MCA occlusion as both the conditioning and the test stimulus. One or three 5-minute episodes of ischemia given 30 minutes before MCA occlusion for 1 or 24 hours (permanent occlusion) confer significant protection as assessed by infarct volume measurements 24 hours later.
Department of Medicine, Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA.
N E
NE
Stagliano
M A
MA
Pérez-Pinzón
M A
MA
Moskowitz
P L
PL
Huang
D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D002536:Cerebral Arteries / Q000473:pathology*; D004195:Disease Models, Animal; D019194:Ischemic Preconditioning; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic