Changes in the metabolic and contractile characteristics of muscle in male cattle between 10 and 16 months of age.
Samples of semitendinosus muscle from 28 male cattle (18 Salers and 10 Limousins) were taken at 10 months (biopsy) and at 16 months of age (at slaughter). The animals had received the same diet and were slaughtered after the same duration of fattening. The activities of isocitrate dehydrogenase and lactate dehydrogenase were measured in the muscle samples. The five lactate dehydrogenase isoenzymes were separated by electrophoresis under non-denaturing conditions and assayed by densitometry. Fibres were identified by histochemistry by myofibrillar ATPase and succinate dehydrogenase activities as SO (slow oxidative), FOG (fast oxidative glycolytic) or FG (fast glycolytic), and by immunohistochemistry by their reaction to monoclonal antibodies specific to slow and fast myosin heavy chain reactions in I, IIC, IIA, IIAB and IIB type fibres. The isocitrate dehydrogenase activity was not modified between 10 and 16 months of age; the lactate dehydrogenase activity decreased and was correlated with an increase in the proportion of the H isozyme to the detriment of the proportion of the M form. This period was characterized by an increase in fibre size, increased expression of MHC IIa, resulting in more IIA fibres, less IIB fibres, and an increase in the percentage of type IIAB fibres, however the proportions of SO, FOG and FG, when analysed statistically, were not modified between 10 and 16 months of age.
Laboratoire Croissance et Métabolisme des Herbivores, Institut National de la Recherche Agronomique, Theix, France.
C
C
Jurie
B
B
Picard
Y
Y
Geay
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002417:Cattle; D007150:Immunohistochemistry; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000145:classification / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000502:physiology; D018995:Myosin Heavy Chains / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases; D018995:Myosin Heavy Chains