Large-scale production of N-acetyllactosamine through bacterial coupling.
A large-scale production system of N-acetyllactosamine, a core structure of various oligosaccharides, was established by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. Two recombinant E. coli strains over-expressed the UDP-Gal biosynthetic genes and the beta-(1-->4)-galactosyltransferase gene of Neisseria gonorrhoeae, respectively. C. ammoniagenes contributed the production of UTP from orotic acid. N-Acetyllactosamine was accumulated at 279 mM (107 g L-1) after a 38 h reaction (2.5 L in volume) starting from orotic acid, D-galactose, and 2-acetamido-2-deoxy-D-glucose.
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan. endo.tetsuo@kyowa.co.jp
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Koizumi
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Tabata
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Kakita
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Ozaki
D000117:Acetylglucosamine / Q000378:metabolism; D000606:Amino Sugars / Q000096:biosynthesis*; D003352:Corynebacterium / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005686:Galactokinase / Q000235:genetics / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D009963:Orotic Acid / Q000378:metabolism; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000235:genetics / Q000378:metabolism; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000235:genetics / Q000378:metabolism
D000606:Amino Sugars; D011993:Recombinant Fusion Proteins; C000458:N-acetyllactosamine; D009963:Orotic Acid; D005700:Galactosyltransferases; C045097:UDP-galactose-lactosylceramide alpha 1-4-galactosyltransferase; D005686:Galactokinase; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D011755:Pyrophosphatases; D000117:Acetylglucosamine; D005690:Galactose