In vitro stimulation of human gingival epithelial cell attachment to dentin by surface conditioning.
BACKGROUND
Chemical root conditioning is widely used to improve the outcome of regenerative periodontal therapies by favoring the attachment of the regenerated periodontal structures. Although the effect of root conditioning on periodontal mesenchymal cells is well documented, very little is known about its potential effect on the re-formation of the junctional epithelium, a crucial event for the protection of the wound. The goal of the present study was to test in vitro the consequences of dentin conditioning with citric acid or minocycline on the attachment kinetics and morphology of human gingival keratinocytes (HGK).


METHODS
The attachment kinetics of HGK to samples of powdered human dentin (particle size 44 to 76 microm) were examined by use of 3H-labeled cells. The morphology of attached epithelial cells was then determined by scanning electron microscopy (SEM).


RESULTS
When the initial adhesion kinetics of cells on untreated dentin were tested, the percentage of attached HGK proved to be dependent on the number of plated cells and the time of incubation (from 0 to 12 hours). Conditioning the dentin by 3% citric acid or by minocycline-HCl (at 0.01, 0.1, or 2.5%) significantly increased (P <0.005) keratinocyte attachment beyond 6 hours, without notable differences between the 2 substances at any concentration. The attachment kinetics of HGK preincubated for 24 hours by 10 microg/ml minocyline-HCl on untreated dentin was found to be similar to that observed for non-preincubated cells. These results are in agreement with the SEM observations: indeed, the surface conditioning of dentin significantly modified the morphology of attached HGK, whereas the preincubation of these cells with minocyline-HCl did not.


CONCLUSIONS
These results suggest that minocycline-HCl does not exert a direct effect on human gingival epithelial cells. In contrast, conditioning the dentin by citric acid or by minocycline stimulates the attachment of HGK, which could lead to a rapid periodontal healing by favoring the re-formation of a junctional epithelium.
Vanheusden
A J
AJ
Institute of Dentistry, University of Liège, Belgium.
Goffinet
G
G
Zahedi
S
S
Nusgens
B
B
Lapière
C M
CM
Rompen
E H
EH
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D019343:Citric Acid / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects*; D004846:Epithelial Attachment / Q000166:cytology / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008911:Minocycline / Q000494:pharmacology; D008954:Models, Biological; D014092:Tooth Root / Q000187:drug effects
D000900:Anti-Bacterial Agents; D019343:Citric Acid; D008911:Minocycline