CEA-related proteins on human urothelial cell lines of different transformation grades.
CEA family proteins from human urothelial cell lines of different transformation grades were characterized by flow cytometry and Western blotting using monoclonal antibodies: 26/3/13, D14HD11, 9A6 and 4/3/17. The following observations were made: (i) the urothelial cell lines, representing transformation grade III (TGr III, tumorigenic, invasive cells), were characterized by the presence of a component with molecular mass 110-135 kDa, most probably representing biliary glycoprotein (BGP); (ii) BGP was absent in non-tumorigenic and non-invasive TGr II urothelial cell lines; (iii) a protein band with apparent molecular mass 180 kDa, and migrating as a CEA standard was detected in only one of seven urothelial cell lines analyzed; (iv) a broad band of apparent molecular mass migrating at 65-90 kDa, probably representing NCA-50/90, was found in two tumorigenic and invasive cell lines, HCV 29T and Hu 1703He.
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
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Krop-Watorek
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Laskowska
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Salwa
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AG
Kłopocki
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F
Grunert
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M
Ugorski
D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D005434:Flow Cytometry; D006801:Humans; D005810:Multigene Family; D014407:Tumor Cells, Cultured; D019459:Urothelium / Q000378:metabolism*
D002272:Carcinoembryonic Antigen