Electron microscopic autoradiography: an improved procedure using Aclar film and Grid-sticks.
A simple, time saving technique for electron microscopic autoradiography (EMARG) has been developed using both Aclar film and Grid-sticks. Rats were given 3 mg/kg [pyridine5-3H] nicorandil orally. After 15 min, animals were sacrificed, the hearts were removed, and myocardial samples for EMARG were prepared. Silver grains were detected clearly in myofibrils and in mitochondria, all showing well preserved ultrastructure. The use of Grid-sticks in the procedure improves handling of bulk samples.
Tsuchiya
Y
Y
Central Research Laboratories, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Sakai
K
K
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D006352:Heart Ventricles / Q000648:ultrastructure; D007553:Isotope Labeling; D008852:Microradiography / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D008929:Mitochondria, Heart / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure; D020108:Nicorandil / Q000008:administration & dosage; D051381:Rats; D014316:Tritium
D014316:Tritium; D020108:Nicorandil