Degradation of Candida albicans Can1 permease expressed in Saccharomyces cerevisiae.
The Candida albicans amino-acid Can1 permease expressed in Saccharomyces cerevisiae is degraded in the vacuole after internalisation by endocytosis. The CaCan1 inactivation and degradation is slow and not inducible by ammonium ions or 'stress' conditions. Using Saccharomyces cerevisiae mutants defective in ubiquitin-protein ligase and ubiquitin-protein hydrolase we have shown that the degradation of heterologous CaCan1 permease is ubiquitin dependent.
Department of Membrane Transport, Institute of Physiology, Prague, Czech Republic.
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Matĕjcková-Forejtová
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Kinclová
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Sychrová
D026905:Amino Acid Transport Systems; D002176:Candida albicans / Q000201:enzymology*; D004705:Endocytosis; D005656:Fungal Proteins; D005822:Genetic Vectors; D008025:Ligases / Q000172:deficiency / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013997:Time Factors; D044767:Ubiquitin-Protein Ligases
D026905:Amino Acid Transport Systems; C097640:CAN1 protein, Candida albicans; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D044767:Ubiquitin-Protein Ligases; D008025:Ligases