Isolation, expression and analysis of splice variants of a human Ca2+/calmodulin-stimulated phosphodiesterase (PDE1A).
The PDE1A gene encodes a Ca2+/calmodulin-stimulated 3',5'-cyclic nucleotide phosphodiesterase (PDE). We have performed 5' and 3' RACE and identified two additional 5'-splice variants and one additional 3'-splice variant of the human PDE1A gene. The three known 5'-splice variants and the two known 3'-splice variants combine to generate six different PDE1A mRNAs. However, one of the 5'-splice variants exhibits alternate splicing in the 5' untranslated region. Thus the six mRNAs encode four different PDE1A proteins. Recombinant forms of the different human PDE1A isoforms were expressed in Sf9 cells. The kinetic properties and inhibitor sensitivities of the four PDE1A isoforms are very similar to one another.
Snyder
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PB
ICOS Corporation, Bothell, WA 98021, USA. psnyder@icos.com
Florio
V A
VA
Ferguson
K
K
Loughney
K
K
D020413:3' Untranslated Regions; D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020121:5' Untranslated Regions; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D018076:DNA, Complementary; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases; D018411:Spodoptera / Q000166:cytology
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D007527:Isoenzymes; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; C517265:PDE1A protein, human; D015106:3',5'-Cyclic-GMP Phosphodiesterases