Monitoring solution-phase combinatorial library synthesis by capillary electrophoresis.
Capillary electrophoresis has been applied to monitor model reactions in solution-phase combinatorial chemistry. In particular, the simultaneous alkylation reactions of secondary amines with a series of benzyl halides has been investigated. Reactant and product concentrations were monitored using capillary electrophoresis in a non-aqueous buffer system. The simplified sample preparation was a key feature making this an attractive method of analysis. The results demonstrate that capillary electrophoresis is a useful tool for monitoring reactions to determine initial rates, rate constants, and extinction correlation coefficients for quantitative analysis in combinatorial chemistry, and is a broadly applicable technique for the analysis of a variety of organic and bioorganic transformations.
Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, California 92008, USA.
H J
HJ
Gaus
P P
PP
Kung
D
D
Brooks
P D
PD
Cook
L L
LL
Cummins
D000081:Acetamides / Q000737:chemistry; D000478:Alkylation; D000588:Amines / Q000032:analysis* / Q000737:chemistry; D001593:Benzyl Compounds / Q000737:chemistry; D017605:Bromine Compounds / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D007700:Kinetics; D010879:Piperazines / Q000138:chemical synthesis
D000081:Acetamides; D000588:Amines; D001593:Benzyl Compounds; D017605:Bromine Compounds; D010879:Piperazines; C038682:benzyl bromide