Determination of alternariol in tomato paste using solid phase extraction and high-performance liquid chromatography with fluorescence detection.
Alternaria spp. produce a wide variety of toxic metabolites with different chemical structures. Tomato products have been considered a likely source of Alternaria toxins in the human diet because Alternaria is an important spoilage mold of tomatoes. A new method for the determination of these mycotoxins in tomato paste, involving solid phase cartridges for extraction before HPLC fluorescence detection with a reversed phase column and isocratic elution, was developed. The method was demonstrated to be linear in the range 5.2-196 ppb of alternariol (AOH) in tomato paste. Good recoveries were obtained for AOH at all levels assayed (minimum 77.2%). The detection limit of the AOH toxin in real samples of tomato paste was low, 1.93 ppb. The precision of the method was demonstrated with a good repeatability (RSD = 2.98%) and reproducibility (RSD = 9.35%).
Dpto. de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.
C A
CA
Fente
J
J
Jaimez
B I
BI
Vázquez
C M
CM
Franco
A
A
Cepeda
D002138:Calibration; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007783:Lactones / Q000032:analysis* / Q000737:chemistry; D018551:Solanum lycopersicum / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis*
D002800:Cholinesterase Inhibitors; D007783:Lactones; D009183:Mycotoxins; C005197:alternariol