Corticosterone modifies muscarinic receptor immunoreactivity in rat hippocampus.
In the present study we report the effect of corticosterone in the regulation of hippocampal muscarinic acetylcholine receptor immunoreactivity (mAChR-ir) expression in rats. Adrenalectomy (ADX) or a single injection of a mineralocorticoid antagonist RU-28318 (1.0 mg/100 g body weight (b.w.)) in adrenally intact rats 24 h prior to sacrifice revealed an increased mAChR-ir in hippocampal CA1 and CA3 areas. Corticosterone replacement (100 microg/100 g b.w.) prevented the increase in mAChR-ir of ADX animals. However, glucocorticoid receptor antagonist (RU38486) treatment in adrenally intact rats failed to affect the mAChR immunolabeling. These results point to a modulation of muscarinic receptors by corticosterone that is predominantly mediated by the mineralocorticoid receptor.
Department of Animal Physiology, Graduated School of Behavioural and Cognitive Neurosciences, University of Groningen, The Netherlands.
B R
BR
Douma
K
K
Jansen
S M
SM
Korte
B
B
Buwalda
E A
EA
Van der Zee
P G
PG
Luiten
D000109:Acetylcholine / Q000032:analysis / Q000096:biosynthesis*; D000315:Adrenalectomy; D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D006624:Hippocampus / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013148:Spironolactone / Q000031:analogs & derivatives / Q000494:pharmacology
D011976:Receptors, Muscarinic; D013148:Spironolactone; D015735:Mifepristone; C041006:RU 28318; D000109:Acetylcholine; D003345:Corticosterone