[Mass spectrometry in the study of bioclasters of amino acids and peptides].
The study of homo- and heterocluster quasimolecular ions of 20 L-amino acids (A) and five dipeptides by the TOF-PDMS method indicated that the intensity of quasimolecular ions of the corresponding homo-([An + H]+ and [Bm + H]+, where A and B are biomolecules (A, dipeptides), n and m = 1 .... 5) and heteroclusters ([An.Bm + H]+, n and m = 1 .... 5) depends mainly on the hydrophobicity of the constituents of the A cluster. The most intensive peaks of homo- and heterocluster ions were obtained for hydrophobic amino acids: L-Ile, L-Leu, L-Val, and L-Phe, and for dipeptides containing these amino acids. The assumption is made that the stereochemical parameters of heterocluster quasimolecular ions in the TOF-PDMS method are determined by the physicochemical mechanisms involved in the processes of ionization/desorption of biomolecules and do not reflect directly biologically significant interactions of biomolecules in vivo.
Agricultural Institute, Sumy, Ukraine.
V D
VD
Chivanov
A K
AK
Mishnev
S A
SA
Alsenov
V I
VI
Eremenko
I A
IA
Eremenko
N G
NG
Zdoĭma
D000596:Amino Acids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis*
D000596:Amino Acids; D010455:Peptides
Issledovanie bioklasterov aminokislot i peptidov metodom mass-spektrometrii.