Conditionally lethal genes in the N pilus region of plasmid pCU1.
Plasmid genes or regions that are conditionally lethal to Escherichia coli have been called kil and those lethal to Klebsiella but not to E. coli have been called kik. Both classes of genes are found in or close to the N pilus region of the plasmid pCU1 and the closely related plasmid pKM101. Here we describe two new and overlapping lethal genes that are located between kikA and traA of the plasmid pCU1 and display host specificity. KilC is lethal in E. coli and Klebsiella while kikC is lethal only in Klebsiella. The previously identified korA gene is sufficient to override the lethality of kilC in trans or in cis but is insufficient to override kikC. kilC expression in E. coli leads to cell death accompanied by an increase in average cell length without affecting septum formation.
Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, Ontario, K1S 5B6, Canada. martin@mgcheo.med.uottawa.ca
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Holcík
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M
Rodríguez
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Couse
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Cherton-Horvat
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Iyer
D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics*; D010861:Fimbriae, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005804:Genes, Lethal; D005838:Genotype; D007709:Klebsiella / Q000235:genetics*; D016366:Open Reading Frames; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D013045:Species Specificity