Kex2 family endoprotease furin is expressed specifically in pit-region parietal cells of the rat gastric mucosa.
The proprotein-processing endoprotease furin is localized in the gastric epithelial cells of the pit region in the rat gastric gland. The gastric pit is composed of several cell types, including gastric surface mucosal (GSM) cells and parietal cells. Furin converts many growth- or differentiation-related proproteins to their active forms. We examined identification of furin-positive cells by immunostaining of rat gastric mucosa and regulators of the furin expression by measuring the furin promoter activity by luciferase assay. Furin-positive cells were stained for H(+)-K(+)-ATPase, indicating that they are parietal cells. Furin-positive parietal cells were not stained for transforming growth factor-alpha (TGF-alpha) but were surrounded by TGF-alpha-positive GSM cells. In contrast, parietal cells below the proliferative zone were positive for TGF-alpha but not for furin. Furin-positive parietal cells expressed a high level of epidermal growth factor receptor (EGFR). TGF-alpha stimulated the furin promoter activity highly in a mouse GSM cell line GSM06. Thus we suggest that the parietal cells of the pit region have furin-mediated functions that can be stimulated by EGFR signaling.
Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371, Japan.
H
H
Kamimura
Y
Y
Konda
H
H
Yokota
S
S
Takenoshita
Y
Y
Nagamachi
H
H
Kuwano
T
T
Takeuchi
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000378:metabolism; D045683:Furin; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010295:Parietal Cells, Gastric / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013381:Subtilisins / Q000378:metabolism*; D014018:Tissue Distribution / Q000502:physiology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
D016211:Transforming Growth Factor alpha; D066246:ErbB Receptors; D013381:Subtilisins; D045683:Furin