Genetic clustering of bovine viral diarrhoea viruses in cattle farms: genetic identification and analysis of viruses directly from cattle sera.
The herd-specific genetic clustering of bovine viral diarrhoea virus (BVDV) was studied by phylogenetic analysis of 42 sera collected between 1995-97 from persistently infected cattle on 16 farms in Sweden. The viruses were typed by sequencing a part of the 5' untranslated region of the genome, which had been amplified directly from serum by reverse transcription-polymerase chain reaction. All of the viruses were of BVDV I, either BVDV Ia (NADL-like) or BVDV Ib (Osloss-like) genotypes. No relationship was observed between the geographic region of origin and the character of clinical signs and the typing of the BVDV isolates. However, the phylogenetic analysis revealed a strict herd-specific genetic clustering of the virus. In 15 of the 16 herds, animals were infected with a single strain of BVDV characteristic for that herd. Direct nucleotide sequence analysis from serum can therefore be used as a tool for molecular epizootiology of BVDV infections.
Vilcek
S
S
Department of Virology, National Veterinary Institute, Box 585, Uppsala, S-751 23, Sweden.
Alenius
S
S
Paton
D J
DJ
Mittelholzer
C
C
Belák
S
S
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000235:genetics*; D002417:Cattle; D004279:DNA, Viral / Q000737:chemistry; D001908:Diarrhea Viruses, Bovine Viral / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004197:Disease Reservoirs; D005260:Female; D005810:Multigene Family; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013548:Sweden
D004279:DNA, Viral; D012367:RNA, Viral