Three enzymes newly identified from the genus Eimeria and two more newly identified from E. maxima, leading to the discovery of some aliphatic acids with activity against coccidia of the domesticated fowl.
Nine enzymes were detected in sporulated oocysts of Eimeria tenella and E. maxima, parasites of the domesticated fowl (Gallus gallus). Three enzymes, hydroxybutyrate dehydrogenase, alanine aminotransferase and gamma-glutamyltransferase, all identified for the first time in Eimeria of fowl, occurred both in E. tenella and in E. maxima. The remaining enzymes assayed had previously been found in various Eimeria species of fowl, although creatine kinase and glutamate dehydrogenase were hitherto unknown from E. maxima. The three enzymes newly recorded from Eimeria of fowl are of interest as potential genetic markers, and also as potential chemotherapeutic targets. The discovery of hydroxybutyrate dehydrogenase led to the demonstration of anticoccidial activity by some aliphatic acids. The paper also includes a list of the enzymes detected in Eimeria of fowl in previous studies.
Williams
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Wellcome Research Laboratories, Berkhamsted, Hertfordshire, UK.
D015720:5'-Nucleotidase / Q000032:analysis; D000410:Alanine Transaminase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D002645:Chickens; D003048:Coccidiosis / Q000188:drug therapy / Q000201:enzymology*; D003049:Coccidiostats / Q000627:therapeutic use; D003402:Creatine Kinase / Q000032:analysis; D016786:Eimeria tenella / Q000201:enzymology*; D005227:Fatty Acids / Q000627:therapeutic use*; D005243:Feces / Q000201:enzymology / Q000469:parasitology; D005969:Glutamate Dehydrogenase / Q000032:analysis; D006884:Hydroxybutyrate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007931:Leucyl Aminopeptidase / Q000032:analysis; D011201:Poultry Diseases / Q000469:parasitology*; D005723:gamma-Glutamyltransferase / Q000032:analysis
D003049:Coccidiostats; D005227:Fatty Acids; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D007931:Leucyl Aminopeptidase