Human transcription factor hTAF(II)150 (CIF150) is involved in transcriptional regulation of cell cycle progression.
Here we present evidence that CIF150 (hTAF(II)150), the human homolog of Drosophila TAF(II)150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle. Gel filtration experiments demonstrate that CIF150 (hTAF(II)150) seems to be less tightly associated with human transcription factor IID than hTAF(II)130 is associated with hTAF(II)250. The transient functional knockout of CIF150 (hTAF(II)150) protein led to cell cycle arrest at the G(2)/M transition in mammalian cell lines. PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAF(II)150) is required for the transcription of only a subset of RNA polymerase II genes. CIF150 (hTAF(II)150) directly stimulated cyclin B1 and cyclin A transcription in cotransfection assays and in vitro assays, suggesting that the expression of these genes is dependent on CIF150 (hTAF(II)150) function. We defined a CIF150 (hTAF(II)150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin B1 core promoter. These results suggest that one function of CIF150 (hTAF(II)150) is to select specific RNA polymerase II core promoter elements involved in cell cycle progression.
Chiron Corporation, Chiron Technologies, Emeryville, California 94608, USA.
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J
Martin
R
R
Halenbeck
J
J
Kaufmann
D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002850:Chromatography, Gel; D019925:Cyclin A / Q000096:biosynthesis / Q000235:genetics; D019926:Cyclin B / Q000096:biosynthesis / Q000235:genetics; D056744:Cyclin B1; D004268:DNA-Binding Proteins / Q000502:physiology*; D029721:Drosophila Proteins; D016195:G2 Phase; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D018390:Gene Targeting; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008677:Metaphase; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000502:physiology*; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D014162:Transfection
C534699:CCNB1 protein, human; C503698:CycB protein, Drosophila; D019925:Cyclin A; D019926:Cyclin B; D056744:Cyclin B1; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D046911:Macromolecular Substances; D009363:Neoplasm Proteins; C486221:TAF2 protein, human; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D012319:RNA Polymerase II