Heavy metal uptake by polyphosphate bodies in living and killed cells of Plectonema boryanum (cyanophycae).
The study was conducted to determine whether living or killed cell polyphosphate bodies (PPB) would sequester more of several heavy metals. Living and heat- or glutaraldehyde-killed cells were exposed to 20 ppm of Zn, Pb, Mn and Al. Air-dried cells on Formvar-coated grids were first observed in the transmission electron microscope. The unit was then switched to the scanning transmission mode of operation with the spot setting. X-rays were collected on an energy dispersive X-ray spectrometer and mass fractions of the metals were determined. In all cases live cells sequestered a larger amount of the metal than cells killed by boiling or with glutaraldehyde. In all cases the cells killed by glutaraldehyde sequestered more of the heavy metals than cells killed by boiling. The results of the investigation show that PPB in living cells with active uptake systems take up and sequester more of the metals Zn, Pb, Mn and Al than killed cells.
Department of Biological Sciences, Lehman College, City University of New York, Bronx, USA.
M
M
Torres
J
J
Goldberg
T E
TE
Jensen
D000042:Absorption; D000458:Cyanobacteria / Q000378:metabolism* / Q000648:ultrastructure; D006358:Hot Temperature; D019216:Metals, Heavy / Q000493:pharmacokinetics*; D008854:Microscopy, Electron; D015388:Organelles / Q000378:metabolism* / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission
D019216:Metals, Heavy