Use of a clinical Escherichia coli isolate expressing lux genes to study the antimicrobial pharmacodynamics of moxifloxacin.
Escherichia coli isolate 16,906 expressing lux genes was used for real-time monitoring of moxifloxacin effects on bacterial metabolism compared with effects on cell replication. Viable counts showed concentration-dependent killing by moxifloxacin; real-time measurement of bioluminescence on the same cultures showed metabolic activity over 54 h, but with greater inhibition at 1 x MIC than with higher MIC multiples. Post-antibiotic effect was longer when determined using bioluminescence than by viable counts. The control-related effective regrowth time was consistent with both methods. Bioluminescent bacteria provide a rapid and sensitive means for measuring antimicrobial effects on bacterial metabolism.
Department of Biological and Biomedical Science, University of the West of England, Bristol, UK. vyv.salisbury@uwe.ac.uk
V
V
Salisbury
A
A
Pfoestl
H
H
Wiesinger-Mayr
R
R
Lewis
K E
KE
Bowker
A P
AP
MacGowan
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001372:Aza Compounds; D001426:Bacterial Proteins / Q000235:genetics; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D018274:Electroporation; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D024841:Fluoroquinolones; D006801:Humans; D008826:Microbial Sensitivity Tests; D000077266:Moxifloxacin; D010088:Oxidoreductases; D011804:Quinolines
D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D001426:Bacterial Proteins; D024841:Fluoroquinolones; D011804:Quinolines; D010088:Oxidoreductases; C055198:LuxC protein, Bacteria; D000077266:Moxifloxacin