Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum.
The acetan biosynthetic pathway in Acetobacter xylinum is an ideal model system for engineering novel bacterial polysaccharides. To genetically manipulate this pathway, an Acetobacter strain (CKE5), more susceptible to gene-transfer methodologies, was developed. A new gene, aceP, involved in acetan biosynthesis was identified, sequenced and shown to have homology at the amino acid level with beta-D-glucosyl transferases from a number of different organisms. Disruption of aceP in strain CKE5 confirmed the function assigned above and was used to engineer a novel polysaccharide with a pentasaccharide repeat unit.
K J
KJ
Edwards
A J
AJ
Jay
I J
IJ
Colquhoun
V J
VJ
Morris
M J
MJ
Gasson
A M
AM
Griffin
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002240:Carbohydrate Sequence; D005798:Genes, Bacterial; D041822:Gluconacetobacter xylinus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; C068545:acetan; C120750:AceP protein, Acetobacter xylinum; D016695:Glycosyltransferases