Significance of fatty acids in pregnancy-induced immunosuppression.
Pregnancy can exert suppressive effects on chronic inflammatory conditions. We have previously demonstrated a depression in polymorphonuclear leukocyte (PMN) respiratory burst during pregnancy which could explain this amelioration. To elucidate the biochemical mechanism, we have examined PMN phospholipase A2 (PLA2) activity and its relationship to cellular and circulating fatty acids in pregnant women (30 to 34 weeks) and nonpregnant controls. PMN PLA2 activity was determined by arachidonic acid (AA) and leukotriene B4 (LTB4) release, respiratory burst activity was determined by lucigenin-enhanced chemiluminescence, and total serum and PMN fatty acid levels were determined by gas-liquid chromatography. AA release was significantly reduced for pregnancy PMNs in response to N-formyl-met-leu-phe (fMLP) under unprimed and tumor necrosis factor alpha (TNF-alpha)- or interleukin 8-primed conditions. Similarly, LTB4 liberation was significantly reduced in response to fMLP and phorbol myristate acetate in unprimed and TNF-alpha-primed pregnancy PMNs. All major fatty acid classes were altered in the pregnant state. Of these differences in PMNs, oleic acid and alpha-linolenic acid showed a significant increase (13 and 26%, respectively) and stearic acid and AA showed a significant decrease (8 and 30%, respectively). The stearic acid, oleic acid, and AA compositions of all cells analyzed correlated with their corresponding changes in serum fatty acid levels. Crossover serum incubations modified both fatty acid profiles and the PMN respiratory burst accordingly, while individual fatty acid incorporation studies highlighted the importance of polyunsaturated fatty acids for NADPH oxidase efficiency. These findings indicate that the attenuation of PMN function in pregnancy may originate from a reduction in the available pool of cellular fatty acids. Furthermore, this reduction arises as a direct result of a pregnancy-induced shift in circulating fatty acids from polyunsaturated to monounsaturated forms.
Crocker
I
I
Medical Research Centre, University of Nottingham, Nottingham City Hospital NHS Trust, Nottingham NG5 1PB, United Kingdom. Ian.Crocker@pmfmrc.nottingham.ac
Lawson
N
N
Daniels
I
I
Baker
P
P
Fletcher
J
J
D000293:Adolescent; D000328:Adult; D001095:Arachidonic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D007975:Leukotriene B4 / Q000096:biosynthesis; D008163:Luminescent Measurements; D019255:NADPH Oxidases / Q000097:blood; D009504:Neutrophils / Q000201:enzymology; D010741:Phospholipases A / Q000097:blood; D054467:Phospholipases A2; D011247:Pregnancy / Q000276:immunology*; D016897:Respiratory Burst; D012710:Serum Albumin, Bovine / Q000378:metabolism
D001095:Arachidonic Acids; D005227:Fatty Acids; D007975:Leukotriene B4; D012710:Serum Albumin, Bovine; D019255:NADPH Oxidases; D010741:Phospholipases A; D054467:Phospholipases A2