A multi-laboratory evaluation of cryopreserved monkey hepatocyte functions for use in pharmaco-toxicology.
Ethical, economic and technical reasons hinder regular supply of freshly isolated hepatocytes from higher mammals such as monkey for preclinical evaluation of drugs. Hence, we aimed at developing optimal and reproducible protocols to cryopreserve and thaw parenchymal liver cells from this major toxicological species. Before the routine use of these protocols, we validated them through a multi-laboratory study. Dissociation of the whole animal liver resulted in obtaining 1-5 billion parenchymal cells with a viability of about 86%. An appropriate fraction (around 20%) of the freshly isolated cells was immediately set in primary culture and various hepato-specific tests were performed to examine their metabolic, biochemical and toxicological functions as well as their ultrastructural characteristics. The major part of the hepatocytes was frozen and their functionality checked using the same parameters after thawing. The characterization of fresh and thawed monkey hepatocytes demonstrated the maintenance of various hepato-specific functions. Indeed, cryopreserved hepatocytes were able to survive and to function in culture as well as their fresh counterparts. The ability for synthesis (proteins, ATP, GSH) and conjugation and secretion of biliary acids was preserved after deep freeze storage. A better stability of drug metabolizing activities than in rodent hepatocytes was observed in monkey. After thawing, Phase I and Phase II activities (cytochrome P450, ethoxycoumarin-O-deethylase, aldrin epoxidase, epoxide hydrolase, glutathione transferase, glutathione reductase and glutathione peroxidase) were well preserved. The metabolic patterns of several drugs were qualitatively and quantitatively similar before and after cryopreservation. Lastly, cytotoxicity tests suggested that the freezing/thawing steps did not change cell sensitivity to toxic compounds.
INSERM/Centre de Recherche Agronomique, Antibes, France.
G
G
de Sousa
F
F
Nicolas
M
M
Placidi
R
R
Rahmani
M
M
Benicourt
B
B
Vannier
G
G
Lorenzon
K
K
Mertens
S
S
Coecke
A
A
Callaerts
V
V
Rogiers
S
S
Khan
P
P
Roberts
P
P
Skett
A
A
Fautrel
C
C
Chesne
A
A
Guillouzo
D000255:Adenosine Triphosphate / Q000378:metabolism; D000655:Amodiaquine / Q000633:toxicity; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004798:Enzymes / Q000378:metabolism; D004917:Erythromycin / Q000633:toxicity; D005069:Evaluation Studies as Topic; D005665:Furosemide / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008252:Macaca fascicularis; D008297:Male; D009926:Organ Preservation; D018675:Toxicity Tests
D004798:Enzymes; D000655:Amodiaquine; D004917:Erythromycin; D005665:Furosemide; D000255:Adenosine Triphosphate; D005978:Glutathione