Morphological domains of Lewis-X/FORSE-1 immunolabeling in the embryonic neural tube are due to developmental regulation of cell surface carbohydrate expression.
The Lewis-X (LeX) carbohydrate epitope, recognized by the FORSE-1 monoclonal antibody (mAb), shares expression boundaries with neural regulatory genes and may be involved in patterning the neural tube by creating domains of differential cell adhesion. The present experiments focus on the question of what determines the expression pattern of LeX in embryonic rat brain. Comparisons of FORSE-1-positive glycolipid and protein antigens in embryonic, early postnatal, and adult tissues show that the LeX epitope is carried primarily by glycolipids during embryonic development and by a proteoglycan and glycoproteins in postnatal and adult tissue. Immunohistochemistry using FORSE-1 and an antibody to the proteoglycan phosphacan, which carries LeX, shows that the distribution of LeX is more restricted than phosphacan. These observations suggest that the precise spatial regulation of FORSE-1 binding in the embryonic forebrain is due to the expression pattern of the LeX carbohydrate on glycolipids, rather than to the transcriptional regulation of a carrier protein.
Division of Biology, 216-76, California Institute of Technology, Pasadena, California, 91125, USA.
K L
KL
Allendoerfer
A
A
Durairaj
G A
GA
Matthews
P H
PH
Patterson
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis / Q000276:immunology; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000276:immunology; D009420:Nervous System / Q000196:embryology*; D009424:Nervous System Physiological Phenomena; D011247:Pregnancy; D011509:Proteoglycans / Q000096:biosynthesis* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016256:Lewis X Antigen; D011509:Proteoglycans; C092260:antigen FORSE-1