The respiratory burst-inhibiting acid phosphatase AcpA is not essential for the intramacrophage growth or virulence of Francisella novicida.
Acid phosphatases capable of inhibiting the respiratory burst of neutrophils have been identified in certain intracellular pathogens. Here we evaluate the role of AcpA, a respiratory burst-inhibiting acid phosphatase of Francisella, in the virulence and intracellular growth of this organism. An F. novicida acpA null mutant was created and found to exhibit wild-type growth kinetics in both cell-line and inflammatory mouse macrophages. The acpA mutant also shows wild-type replication in the spleens of experimentally infected mice. These data suggest that AcpA is not essential for the intracellular growth or virulence of F. novicida.
Department of Biochemistry and Microbiology, University of Victoria, B.C., Canada.
G S
GS
Baron
T J
TJ
Reilly
F E
FE
Nano
D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D002460:Cell Line; D015169:Colony Count, Microbial; D005260:Female; D005603:Francisella / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D016897:Respiratory Burst; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000382:microbiology; D013997:Time Factors; D015534:Trans-Activators / Q000502:physiology*; D014774:Virulence / Q000502:physiology
C091636:AcpA protein, Bacillus anthracis; D001426:Bacterial Proteins; D015534:Trans-Activators