Osteoblast behaviour in the presence of bisphosphonates: ultrastructural and biochemical in vitro studies.
OBJECTIVE
A positive balance in bone remodelling is an important goal of bone metabolism both in the presence of the osteoporotic processes characteristic of ageing and, especially, of prosthetic implants. The aim of the present work was to obtain new information about the initial steps of osteoblastic growth in an in vitro osteoblastic model in the presence of two bisphosphonates.


METHODS
Experiments were performed with Alendronate and Neridronate, two molecules used in the therapy of osteoporosis. Since differentiating features into osteoblastic cells are known to parallel the presence in the cytoplasm of alkaline phosphatase and osteocalcin, we also carried out immunohistochemical typing.


RESULTS
Good differentiation and osteoblastic activity were generally observed in the cells in contact with these compounds, except for 10(-4) Neridronate, where biochemical data clearly indicated its toxic effect on the cells.


CONCLUSIONS
The detection of osteoblastic markers associated with an ultrastructural picture of correct organellar morphology in our cultures further supports the hypothesis of a metabolically positive action of these molecules on osteoblasts.
Gandolfi
M G
MG
Institute of Histology, University of Bologna, Italy.
Pugnaloni
A
A
Mattioli-Belmonte
M
M
Muzzarelli
R
R
De Benedittis
A
A
Mengucci
P
P
Zucchini
C
C
Tesei
M
M
Caudarella
R
R
Biagini
G
G
D019386:Alendronate / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D004164:Diphosphonates / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure
D015415:Biomarkers; D004164:Diphosphonates; C053389:6-amino-1-hydroxyhexane-1,1-diphosphonate; D000469:Alkaline Phosphatase; D019386:Alendronate