Synthesis of a monocharged peptide nucleic acid (PNA) analog and its recognition as substrate by DNA polymerases.
The preparation of a novel phosphoramidite monomer based on thyminyl acetic acid coupled to the secondary nitrogen of 2-(2-amino-ethylamino)ethanol is described. This monomer can be used to attach a deoxynucleotide to the carboxy terminus of a PNA oligomer by solid-phase synthesis. The resulting PNA primer is recognized as a substrate by various DNA polymerases.
Swiss Federal Institute of Technology, Department of Chemistry, Zürich, Switzerland.
M J
MJ
Lutz
D W
DW
Will
G
G
Breipohl
S A
SA
Benner
E
E
Uhlmann
D001483:Base Sequence; D017931:DNA Primers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D017931:DNA Primers; D007202:Indicators and Reagents; D020135:Peptide Nucleic Acids; D004259:DNA-Directed DNA Polymerase