Effects of subconjunctival injection of mitomycin-C on iridial circulation in rabbits.
OBJECTIVE
To determine the effects of a subconjunctival injection of mitomycin-C (MMC) on iridial circulation in rabbits.


METHODS
Dutch rabbits anesthetized with pentobarbital received a 0.2-ml subconjunctival injection of 0.4 mg/ml or 0.1 mg/ml MMC; the contralateral eye received 0.2 ml physiological saline. Intraocular pressure (IOP) and NB( iris), a quantitative index of iridial tissue blood velocity, were measured up to 24 hours after treatment.


RESULTS
At a dosage of 0.4 mg/ml MMC, NB iris obtained from the iridial area adjacent to the injection site decreased significantly by 13.6% and 18.6% at 1 and 2 hours (P = 0.03, 0.01, respectively) after treatment; NB iris obtained on the contralateral side of the injection showed no significant change. In eyes treated with 0.1 mg/ml MMC, NB iris did not change significantly. The mean IOP was significantly lower by 3.1, 3.0, and 1.8 mm Hg at 6, 12, and 24 hours after the 0.4 mg/ml injection of MMC compared with the fellow eyes (P = 0.03, 0.01, and 0.03, respectively), the IOP decreased by 1.7 mm Hg at 4 hours in the eyes treated with 0.1 mg/ml MMC (P = 0. 04).


CONCLUSIONS
Subconjunctival injections of 0.2 ml of 0.4 mg/ ml MMC caused not only a significant decrease of IOP but also a transient but significant effect on iridial circulation.
Omiya Red Cross Hospital Eye Clinic Japan Saitama, 338-8553,
A
A
Tomidokoro
M
M
Araie
Y
Y
Tamaki
K
K
Muta
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology; D001783:Blood Flow Velocity / Q000187:drug effects; D003228:Conjunctiva; D007267:Injections; D007429:Intraocular Pressure / Q000187:drug effects; D007498:Iris / Q000098:blood supply*; D016685:Mitomycin / Q000008:administration & dosage* / Q000494:pharmacology; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin