Relative amounts of mRNA encoding four subtypes of muscarinic receptors (m2-m5) in human peripheral blood mononuclear cells.
It is known that lymphocytes express functional muscarinic cholinergic receptors. In this study, RT-PCR method was applied to study the presence and relative levels of mRNA encoding muscarinic receptor subtypes in human peripheral blood mononuclear cells (PBMCs). Our results, confirmed by DNA sequencing, demonstrate the presence of m2, m3, m4, and m5 receptor subtypes in human PBMCs. The relative levels of muscarinic receptor subtypes fit the following pattern: m3 > m5 > m4 > m2. Our data provide strong evidence confirming previous pharmacological studies that suggested the existence of several subtypes of muscarinic receptors on human PBMCs. We cannot exclude the possibility that expression of receptor subtype depends on the lineage and/or activation status of the cell.
Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland.
U
U
Bany
J
J
Ryzewski
W
W
Maśliński
D018679:Cholinergic Agonists / Q000494:pharmacology; D017931:DNA Primers; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D015213:Neuroimmunomodulation / Q000235:genetics / Q000276:immunology; D010275:Parasympathetic Nervous System / Q000737:chemistry / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D043588:Receptor, Muscarinic M4; D043642:Receptor, Muscarinic M5; D011976:Receptors, Muscarinic / Q000235:genetics* / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018679:Cholinergic Agonists; D017931:DNA Primers; D012333:RNA, Messenger; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D043588:Receptor, Muscarinic M4; D043642:Receptor, Muscarinic M5; D011976:Receptors, Muscarinic