Absence of correlation between telomerase activity and hepatic neoplasia in B6C3F1 mice.
Telomeres are the physical ends of eukaryotic chromosomes, which maintain chromosome stability and are progressively shortened with aging in somatic cells. The enzyme telomerase elongates telometric DNA and while not usually detectable in human somatic cells is expressed in most human tumors. The present study was conducted to determine if telomerase activity is a marker for spontaneous hepatic neoplastic changes in B6C3F1 mice, a strain frequently used in rodent carcinogenicity studies. Telomerase activity was generally higher in microscopically normal liver tissue from 8-week-old compared to aged mice (110-week-old); however, telomerase activity was not consistently increased in hepatocellular adenomas and carcinomas. It is proposed that, while elevated telomerase activity may modulate human tumor development, modulation of telomerase activity is not a feature of hepatic tumors in B6C3F1 mice and therefore is unlikely to have utility as a molecular marker for hepatic neoplasia in this mouse strain.
Parke Davis Research Institute, Mississauga, Canada. lena.king@wl.com
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King
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Song
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Wojcinski
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Baker
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Walker
D000818:Animals; D005260:Female; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D051379:Mice; D019098:Telomerase / Q000378:metabolism*
D019098:Telomerase