Cell tropism of Staphylococcus aureus in bovine mammary gland cell cultures.
Staphylococcus aureus is one of the most important pathogens of the bovine mammary gland. The interaction of S. aureus with cells of the bovine mammary gland is considered to play an essential role in the pathogenesis. In this study, we identified a new target cell for S. aureus adhesion and invasion. For that purpose, cells which compose the alveoli of the mammary gland were cultured. In these cultures, two morphologically different cell types, elongated and cubic cells, were observed. Adhesion and invasion of S. aureus was studied using microscopical and microbiological methods. S. aureus adhered specifically and in large numbers (about 300 bacteria/cell) to the elongated cell type. No adhesion to the cubic cell type was observed. In addition, bacteria were also found intracellularly in the elongated cells, and enclosed in membrane vesicles. Adhesion and invasion were time dependent and reached maximum levels after 4 h. Invasion was strongly reduced by staurosporine and genistein. The newly identified target cell was further characterized.
Department of Bacteriology, DLO-Institute for Animal Science and Health, Lelystad, The Netherlands. a.lammers@id.dlo.nl
A
A
Lammers
P J
PJ
Nuijten
E
E
Kruijt
N
N
Stockhofe-Zurwieden
U
U
Vecht
H E
HE
Smith
F G
FG
van Zijderveld
D000818:Animals; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion / Q000502:physiology; D002417:Cattle; D018929:Cell Culture Techniques; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry; D007774:Lactation; D008321:Mammary Glands, Animal / Q000473:pathology; D008414:Mastitis, Bovine / Q000503:physiopathology*; D008854:Microscopy, Electron / Q000662:veterinary; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000503:physiopathology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000472:pathogenicity; D019311:Staurosporine / Q000494:pharmacology; D018523:Tropism / Q000502:physiology
D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D; D019311:Staurosporine; D003078:Colchicine