Fibrinogen adsorbs from aqueous media to microscopic droplets of poly(dimethylsiloxane) and remains coagulable.
Fibrinogen binds from aqueous media containing it to droplets of linear trimethylsilyl-terminated poly(dimethylsiloxane) (PDMS) dispersed in those same media. Once bound, fibrinogen elutes from emulsified droplets of PDMS only very slowly, even when incubated in buffer that contains a physiologic concentration of the protein. The bound fibrinogen is coagulable, as indicated by the thrombin-dependent agglutination of droplets. Thus fibrinogen bound to droplets of PDMS renders an adhesive potential to the surface of the droplets, a potential that may have relevance to the biologic processing of the polymer in vivo.
Department of Materials Science and Engineering and the Polymer Research Center, University of Cincinnati, Cincinnati, Ohio 45221-0012, USA.
P W
PW
Whitlock
S J
SJ
Clarson
G S
GS
Retzinger
D000268:Adhesiveness; D000327:Adsorption; D000371:Agglutination; D001777:Blood Coagulation / Q000502:physiology*; D001798:Blood Proteins / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004655:Emulsions; D005340:Fibrinogen / Q000737:chemistry*; D007202:Indicators and Reagents; D010316:Particle Size; D012828:Silicones / Q000737:chemistry*; D014867:Water
D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D004655:Emulsions; D007202:Indicators and Reagents; D012828:Silicones; D014867:Water; C013830:baysilon; D005340:Fibrinogen