Severe ocular abnormalities in C57BL/6 but not in 129/Sv p53-deficient mice.
OBJECTIVE
To demonstrate the importance of genetic background interaction on the development of ocular phenotypes in p53-deficient mice.


METHODS
Eyes of adult mice, homozygous and heterozygous for the p53 gene disruption in the 129/SvJ and C57BL/6J (B6) genetic backgrounds, and their F1 progeny were examined by indirect ophthalmoscopy and by light microscopy.


RESULTS
Indirect ophthalmoscopy revealed unilateral or bilateral vitreal opacities, fibrous retrolental tissue, and retinal folds in adult B6 mice but not in 129/Sv mice homozygous for a p53 null mutation. In B6 p53-/- mice, blood vessels extended from the peripapillary inner retina through the posterior vitreous and into the retrolental membrane. Optic nerves were hypoplastic.


CONCLUSIONS
These findings indicate that alleles from the B6 background contribute to the aberrant ocular phenotypes observed in p53 deficiency. They also suggest that p53 or the pathway in which it functions may be important for normal eye development.
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
S
S
Ikeda
N L
NL
Hawes
B
B
Chang
C S
CS
Avery
R S
RS
Smith
P M
PM
Nishina
D000015:Abnormalities, Multiple / Q000235:genetics* / Q000473:pathology; D000818:Animals; D002386:Cataract / Q000235:genetics / Q000473:pathology; D017931:DNA Primers / Q000737:chemistry; D005124:Eye Abnormalities / Q000235:genetics* / Q000473:pathology; D005128:Eye Diseases / Q000235:genetics / Q000473:pathology; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009900:Optic Nerve / Q000002:abnormalities / Q000473:pathology; D010641:Phenotype; D012160:Retina / Q000002:abnormalities* / Q000473:pathology; D015792:Retinal Dysplasia / Q000235:genetics / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics; D014822:Vitreous Body / Q000002:abnormalities* / Q000473:pathology
D017931:DNA Primers; D016159:Tumor Suppressor Protein p53