Determination of fenoprofen in serum by capillary isotachophoresis.
A isotachophoretic method with conductivity detection was developed and validated to directly determine fenoprofen in human serum. The leading electrolyte contained hydrochloric acid (10 mmol/l), 6-aminocaproic acid (pH 4.8) and polyvinylpyrrolidone (0.1%). The terminating electrolyte was 4-morpholineethanesulfonic acid (5 mmol/l). The calibration curve was linear over the concentration range 0.02-0.40 mmol/l. Within-day standard deviation ranged from 0.001 to 0.004 and between-day standard deviation ranged from 0.001 to 0.004. The limit of determination was 0.02 mmol/l. The assay was employed to determine serum concentration of fenoprofen in patients.
Department of Analytical Chemistry, Faculty of Chemical Technology, Bratislava, Slovak Republic.
J
J
Sádecká
A
A
Hercegová
J
J
Polonský
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002138:Calibration; D004586:Electrophoresis / Q000379:methods*; D005279:Fenoprofen / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005279:Fenoprofen