Effect of the bacillus of Calmette-Guérin, Propionibacter acnes and avridine as immunomodulators in antirabies vaccination of mice using the Fuenzalida-Palacios mouse brain vaccine.
Using the laboratory mice, Fuenzalida-Palacios mouse brain human rabies vaccine was administered in groups of animals previously inoculated with rabies virus and then submitted to treatments with the immunomodulators onco-BCG, avridine and Propionibacterium acnes. Humoral and cellular immune responses were evaluated through the macrophage inhibition factor (MIF), intra-pad inoculation (IPI) and serum neutralization (SN) tests and by the detection of gamma-interferon (IFN-gamma). The IPI test was not effective in detecting the response of delayed-type hypersensitivity, contrary to MIF, which showed the immune cellular response. Higher levels of IFN-gamma were observed in the groups of mice vaccinated and treated with avridine and P. acnes. Although immunomodulating activities have been detected, the use of adjuvants with the Fuenzalida-Palacios type vaccine in mice did not reveal any encouraging results.
Department of Veterinary Hygiene and Public Health, FMVZ, UNESP, Botucatu, SP, Brazil. jane@fmvz.unesp.br
J
J
Megid
M T
MT
Peraçolli
P R
PR
Curi
C R
CR
Zanetti
W H
WH
Cabrera
R
R
Vassao
F H
FH
Ito
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000998:Antiviral Agents / Q000276:immunology; D001500:BCG Vaccine / Q000276:immunology*; D001921:Brain / Q000821:virology; D003959:Diamines / Q000276:immunology*; D005260:Female; D006801:Humans; D007273:Injections, Intramuscular; D007369:Interferon Inducers / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D011425:Propionibacterium acnes / Q000276:immunology*; D011818:Rabies / Q000276:immunology* / Q000517:prevention & control; D011819:Rabies Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000998:Antiviral Agents; D001500:BCG Vaccine; D003959:Diamines; D007369:Interferon Inducers; D011819:Rabies Vaccines; C006270:avridine