Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus.
We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV. Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a "zinc finger"-like motif.
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
K
K
Nakamura
K
K
Iwatsuki
Y
Y
Izumiya
Y
Y
Nishimura
K
K
Ohashi
T
T
Mikami
C
C
Kai
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D004285:Dogs; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D012302:Rinderpest virus / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D010750:Phosphoproteins; D014764:Viral Proteins; C076637:P gene protein, Rinderpest virus