A protein linked to mitochondrion development in Trypanosoma brucei.
The use of the two-hybrid system in yeast allowed us to isolate a new mitochondrial protein of Trypanosoma brucei, termed PIE8, for putative protein interacting with ESAG8. This protein was found to localize progressively in the single mitochondrion of the parasite during the mitochondrial reactivation needed to adapt the parasite from the glycolysis-based metabolism in the mammalian host, to the cytochrome-mediated respiration in the fly vector. Once this reactivation is established, PIE8 is lost from the mitochondrion. Thus, the temporary presence of PIE8 in the mitochondrion is linked to mitochondrial reactivation.
Department of Molecular Biology, Free University of Brussels, Genèse, Belgium. dperez@alize.ulb.ac.be
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D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017125:Genes, Protozoan; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014346:Trypanosoma brucei brucei / Q000254:growth & development* / Q000648:ultrastructure
D018076:DNA, Complementary; D024101:Mitochondrial Proteins; C121454:PIE8 protein, Trypanosoma brucei; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; C066671:ESAG 8 protein, Trypanosoma brucei