Monitoring rotavirus environmental contamination in a pediatric unit using polymerase chain reaction.
Rotavirus environmental contamination in a pediatric unit was investigated. Surfaces were swabbed, then viruses eluted, ultracentrifuged, and detected by polymerase chain reaction (PCR) amplification. Of 55 samples, 25 (46%) tested positive. Rotavirus RNA was more prevalent on surfaces in direct contact with children (thermometers and play mats) than on other environmental surfaces (washbasins, door handles, etc). PCR has proved useful for monitoring rotavirus environmental contamination.
Soule
H
H
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, France.
Genoulaz
O
O
Gratacap-Cavallier
B
B
Mallaret
M R
MR
Morand
P
P
François
P
P
Luu Duc Bin
D
D
Charvier
A
A
Bost-Bru
C
C
Seigneurin
J M
JM
D002648:Child; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004783:Environmental Microbiology; D004784:Environmental Monitoring / Q000379:methods*; D004866:Equipment Contamination; D006785:Hospitals, University; D006801:Humans; D007231:Infant, Newborn; D017053:Infection Control / Q000379:methods; D015278:Intensive Care Units, Pediatric; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000517:prevention & control
D017931:DNA Primers; D012367:RNA, Viral