Stimulation of cellular XTT reduction by cytochrome oxidase inhibitors.
XTT reducing activity by CHO and L1210 cells was found to be stimulated by the presence of cytochrome oxidase inhibitors such as NaN3 or KCN. Among the other respiratory chain inhibitors, antimycin A (a complex III inhibitor) and chlorpromazine inhibited cellular XTT reduction, and rotenone and malonate showed slight inhibition and no effect, respectively. It is suggested that XTT reduction is coupled with the respiratory chain via cytochrome c, which is located between complexes III and IV (cytochrome oxidase).
Institute of Microbial Chemistry, Tokyo, Japan.
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Kunimoto
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Nosaka
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Takeuchi
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000494:pharmacology; D019810:Sodium Azide / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism*
D004791:Enzyme Inhibitors; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D019810:Sodium Azide; D003576:Electron Transport Complex IV; D011190:Potassium Cyanide