High resolution multicolor-banding: a new technique for refined FISH analysis of human chromosomes.
A new multicolor-banding technique has been developed which allows the differentiation of chromosome region specific areas at the band level. This technique is based on the use of differently labeled overlapping microdissection libraries. The changing fluorescence intensity ratios along the chromosomes are used to assign different pseudo-colors to specific chromosome regions. The multicolor banding of human chromosome 5 is presented as an example.
Institute of Human Genetics and Anthropology, Jena, Germany. ic@metasystems.de
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I
Chudoba
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Plesch
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Lörch
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J
Lemke
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Claussen
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Senger
D002871:Chromosome Banding / Q000379:methods*; D002872:Chromosome Deletion; D020223:Chromosome Painting / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics*; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005456:Fluorescent Dyes; D015698:Genomic Library; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015342:DNA Probes; D005456:Fluorescent Dyes