Impairment of the correlation between urinary contents of alpha-1-microglobulin and ulinastatin is induced by intracerebroventricularly administered interleukin-6 in mice.
We have found previously that the correlation between urinary contents of alpha-1-microglobulin (alpha1M) and ulinastatin (UT) depends on the type of neuropsychiatric disease. Since interleukin (IL)-1beta and IL-6 are closely involved in pathophysiological aspects of various neuropsychiatric diseases, effects of intracerebroventricularly (i.c.v.) administered IL-1beta and IL-6 on the correlation between urinary contents of these two glycoproteins were examined in mice, a species in which alpha1M and UT and also the correlation between the urinary contents thereof are expressed similarly to humans. Indices (volume, contents of creatinine, alpha1M and UT, and alpha1M/UT ratio) in urine collected after i.c.v. administrations of 2 and 20 ng of either IL-1beta or IL-6 were not statistically different from those of the vehicle-treated (control) groups. Neither IL-1beta (2 and 20 ng) nor the lower dose of IL-6 (2 ng) affected the positive correlation between urinary contents of alpha1M and UT. However, a higher dose of IL-6 (20 ng) nullified the positive correlation for 2 days after administration. Recovery to a positive correlation was thereafter displayed. These findings suggest that central IL-6 plays an important role in correlating urinary contents of alpha1M and UT without affecting the renal functions.
Department of Psychiatry, Shimane Medical University, Izumo, Japan.
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K
Kaku
T
T
Shikimi
T
T
Inagaki
H
H
Ishino
H
H
Okunishi
S
S
Takaori
D000510:Alpha-Globulins / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000652:urine; D006023:Glycoproteins / Q000652:urine*; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000008:administration & dosage / Q000378:metabolism; D015850:Interleukin-6 / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010300:Parkinson Disease / Q000378:metabolism
D000510:Alpha-Globulins; D015415:Biomarkers; D006023:Glycoproteins; D007375:Interleukin-1; D015850:Interleukin-6; C016001:alpha-1-microglobulin; C028665:urinastatin