Simultaneous determination of donepezil (aricept) enantiomers in human plasma by liquid chromatography-electrospray tandem mass spectrometry.
A rapid, sensitive and enantioselective LC-MS-MS method using deuterium-labeled internal standard was developed and evaluated for the simultaneous quantitative determination of donepezil enantiomers in human plasma without interconversion during clean-up process and measurement. The use of an avidin column allowed the separation of donepezil enantiomers, which were specifically detected by MS-MS without interference from its metabolites and plasma constituents. Evaluation of this assay method shows that samples can be assayed with acceptable accuracy and precision within the range from 0.0206 ng/ml to 51.6 ng/ml for both R-donepezil and S-donepezil. This analytical method was applied to the simultaneous quantitation of donepezil enantiomers in human plasma.
Matsui
K
K
Drug Evaluation Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan.
Oda
Y
Y
Nakata
H
H
Yoshimura
T
T
D002138:Calibration; D002800:Cholinesterase Inhibitors / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D000077265:Donepezil; D006801:Humans; D007189:Indans / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D010880:Piperidines / Q000097:blood*; D015203:Reproducibility of Results; D013237:Stereoisomerism
D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D000077265:Donepezil