Histological study of normal and tumor-bearing liver treated with focused ultrasound.
The purpose of this investigation was to study the tissue damage (including blood vessels) on both normal and tumor-bearing experimental livers and the course of liver repair after focused ultrasound (FUS) treatment using histological evaluation. A series of experiments were carried out in vivo. Tissue was treated using arrays of ultrasound exposures with a frequency of 1.7 MHz, in situ spatially averaged focal intensity (I(SAL) in situ) of 212-266 W/cm2 (corresponding to in situ spatial peak intensity of 382-479 W/cm2), 5-10 s exposure duration and 1.5-3.0 mm exposure separation. Tissue specimens were examined using both light and electron microscopy. The damage to the blood vessel walls was studied. The results showed the existence of indirect tissue damage in both normal and tumor tissue that is outside of the treatment volume, due to disruption of the major blood vessels supplying the adjacent area. Evidence for liver regeneration was found 2 months after FUS treatment.
Joint Department of Physics, Institute of Cancer Research and Royal Marsden Hospital, Sutton, Surrey, UK. Lili@s-word-.stanford.edu
L
L
Chen
G
G
ter Haar
D
D
Robertson
J P
JP
Bensted
C R
CR
Hill
D000818:Animals; D005354:Fibrosarcoma / Q000098:blood supply / Q000473:pathology* / Q000628:therapy*; D008099:Liver / Q000098:blood supply* / Q000293:injuries / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000098:blood supply / Q000473:pathology* / Q000628:therapy*; D008115:Liver Regeneration; D008854:Microscopy, Electron; D051381:Rats; D013997:Time Factors; D014464:Ultrasonic Therapy / Q000295:instrumentation / Q000379:methods; D014465:Ultrasonics / Q000009:adverse effects