Functional analysis of 12 ORFs from Saccharomyces cerevisiae chromosome II.
Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c and YBR211c are essential genes for vegetative growth. No effects were detected in any of the haploid deletion mutants for the rest of the ORFs with respect to growth, gross morphology or mating.
Departament de Bioquímica i Biología Molecular, Facultat de Ciències Biològiques, Universitat de València, Spain. jose.e.perez@uv.es
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S
Rodríguez-Navarro
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Estruch
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Pérez-Ortín
D015825:Chromosomes, Fungal / Q000235:genetics*; D020043:Genes, Essential; D005800:Genes, Fungal; D016366:Open Reading Frames / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development*