Component specificity for the thylakoidal Sec and Delta pH-dependent protein transport pathways.
Prokaryotes and prokaryote-derived thylakoid membranes of chloroplasts share multiple, evolutionarily conserved pathways for protein export. These include the Sec, signal recognition particle (SRP), and Delta pH/Tat systems. Little is known regarding the thylakoid membrane components involved in these pathways. We isolated a cDNA clone to a novel component of the Delta pH pathway, Tha4, and prepared antibodies against pea Tha4, against maize Hcf106, a protein implicated in Delta pH pathway transport by genetic studies, and against cpSecY, the thylakoid homologue of the bacterial SecY translocon protein. These components were localized to the nonappressed thylakoid membranes. Tha4 and Hcf106 were present in approximately 10-fold excess over active translocation sites. Antibodies to either Tha4 or Hcf106 inhibited translocation of four known Delta pH pathway substrate proteins, but not of Sec pathway or SRP pathway substrates. This suggests that Tha4 and Hcf106 operate either in series or as subunits of a heteromultimeric complex. cpSecY antibodies inhibited translocation of Sec pathway substrates but not of Delta pH or SRP pathway substrates. These studies provide the first biochemical evidence that Tha4 and Hcf106 are specific components of the Delta pH pathway and provide one line of evidence that cpSecY is used specifically by the Sec pathway.
Horticultural Sciences and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA.
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D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D029681:Arabidopsis Proteins; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D060365:Chloroplast Proteins; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003001:Cloning, Molecular; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018532:Pisum sativum / Q000166:cytology / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle / Q000502:physiology; D003313:Zea mays / Q000166:cytology / Q000378:metabolism
D000906:Antibodies; D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; C109236:Hcf106 protein, plant; D008565:Membrane Proteins; D010940:Plant Proteins; C000613370:SCY1 protein, Arabidopsis; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle; C492348:Tha4 protein, Arabidopsis; C120444:Tha4 protein, plant