Quantification of cellular properties from external fields and resulting induced velocity: magnetic susceptibility.
An experimental technique is discussed in which the magnetic susceptibility of immunomagnetically labeled cells can be determined on a cell-by-cell basis. This technique is based on determining the magnetically induced velocity that an immunomagnetically labeled cell has in a well-defined magnetic energy gradient. This velocity is determined through the use of video recordings of microscopic images of cells moving in the magnetic energy gradient. These video images are then computer digitized and processed using a computer algorithm, cell tracking velocimetry, which allows larger numbers (>10(3)) of cells to be analyzed.
Department of Chemical Engineering, The Ohio State University, 140 WEST 19th Avenue, Columbus, Ohio 43210, USA. Chalmers.1@osu.edu
J J
JJ
Chalmers
S
S
Haam
Y
Y
Zhao
K
K
McCloskey
L
L
Moore
M
M
Zborowski
P S
PS
Williams
D002478:Cells, Cultured; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018189:Immunomagnetic Separation; D008214:Lymphocytes / Q000166:cytology; D014407:Tumor Cells, Cultured