Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry.
A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6+/-2.4%. We detected LTE4 (63.1+/-18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples.
Department of Pharmaceutical Science, Tohoku University Hospital, Sendai, Japan. mizugaki@mail.cc.tohoku.ac.jp
M
M
Mizugaki
T
T
Hishinuma
N
N
Suzuki
D000328:Adult; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D017999:Leukotriene E4 / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results
D017999:Leukotriene E4