Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata.
The gene encoding centromere binding factor 3d (CBF3D) of the human pathogenic yeast Candida glabrata has been isolated by hybridization of Saccharomyces cerevisiae CBF3D (ScCBF3D) DNA to a C. glabrata partial genomic library. Sequence analysis revealed a 540 bp open reading frame encoding a protein of 179 amino acids with a calculated molecular mass of 20.9 kDa. The amino acid sequence is highly homologous (78.6% identity) to ScCbf3d and 48.3% identical to the human homologue p19 (SKP1). Southern blot analysis indicates that CgCbf3d is encoded by an unique nuclear gene. The cloned CgCBF3D gene can functionally substitute the S. cerevisiae homologue in a S. cerevisiae CBF3D-deletion mutant. The GenBank Accession No. for this gene is AF 072472.
Institut für Molekulare Biotechnologie, Abt. Molekularbiologie, Beutenbergstrasse 11, 07745 Jena, Germany. stoyan@lifesci.ucsb.edu
T
T
Stoyan
R
R
Eck
J
J
Lechner
P
P
Hemmerich
W
W
Künkel
S
S
Diekmann
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D003429:Cross Reactions; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D044783:F-Box Proteins; D018628:Gene Dosage; D005816:Genetic Complementation Test; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D044843:SKP Cullin F-Box Protein Ligases; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; D044783:F-Box Proteins; D029701:Saccharomyces cerevisiae Proteins; D044843:SKP Cullin F-Box Protein Ligases; C120507:SKP1 protein, S cerevisiae