Identification of human rotavirus strains with the P[14] genotype by PCR.
A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
Dipartimento di Igiene e Microbiologia, Università di Palermo," Palermo, Italy.
S
S
Arista
E
E
Vizzi
C
C
Alaimo
D
D
Palermo
A
A
Cascio
D002213:Capsid / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification