The thermophilic yeast Hansenula polymorpha does not require trehalose synthesis for growth at high temperatures but does for normal acquisition of thermotolerance.
The TPS1 gene from Hansenula polymorpha, which encodes trehalose-6-phosphate (Tre6P) synthase, has been isolated and characterized. The deletion of TPS1 rendered H. polymorpha cells incapable of trehalose synthesis under conditions where wild-type cells normally accumulate high levels of trehalose. Interestingly, the loss of Tre6P synthase did not cause any obvious growth defects on a glucose-containing medium, even at high temperatures, but seriously compromised the cells' ability to acquire thermotolerance.
Botanisches Institut der Universität, CH-4056 Basel, Switzerland.
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Reinders
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Romano
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Wiemken
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De Virgilio
D017353:Gene Deletion; D005816:Genetic Complementation Test; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D010843:Pichia / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D013403:Sugar Phosphates / Q000378:metabolism; D013696:Temperature; D014199:Trehalose / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism
D013403:Sugar Phosphates; C082722:trehalose-6-phosphate; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase