Typing of clinical herpes simplex virus type 1 and type 2 isolates with monoclonal antibodies.
The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoassay. The anti-HSV-1 MAb showed sensitivity and specificity of 100%, and the anti-HSV-2 MAb showed a sensitivity of 99.46% and 100% specificity, indicating that these MAbs are suitable for typing of clinical HSV isolates.
Department of Virology, University of Göteborg, S-413 46 Göteborg, Sweden. jan-ake.liljeqvist@mednet.gu.se
J A
JA
Liljeqvist
B
B
Svennerholm
T
T
Bergström
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D006558:Herpes Genitalis / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006561:Herpes Simplex / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral