Diagnosis of rickettsial diseases using samples dried on blotting paper.
The use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting blood samples for serological studies. In addition, samples occupy little space and can be readily transported without refrigeration. Rickettsial diseases often evolve according to an epidemic mode and are now considered reemerging diseases, especially in developing countries, under conditions where fieldwork could be difficult. The suitability of collecting whole-blood specimens on filter paper discs for rickettsial antibody assay was evaluated. Dried blood specimens from 64 individuals with antibodies to Coxiella burnetii, Bartonella quintana, or Rickettsia conorii were tested for rickettsial antibodies by microimmunofluorescence. Although occasional titers were 1 or 2 dilutions lower than those of tested serum samples, no statistically significant differences were observed. Among patients with negative serology, no false positives were found. This study demonstrated that the recovery of antibodies from finger-stick blood dried on filter paper after elution produces results comparable to those obtained by recovering antibodies from serum. Storing paper samples for 1 month at room temperature or at 4 degrees C did not significantly affect the level of antibodies recovered. This report shows the utility of this sample collection method in developing countries where refrigeration is not possible and venipuncture is problematic.
Fenollar
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Unité des Rickettsies, CNRS:UPRESA 6020, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille France.
Raoult
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D000907:Antibodies, Bacterial / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods*; D005189:False Positive Reactions; D005374:Filtration / Q000295:instrumentation; D005455:Fluorescent Antibody Technique; D006801:Humans; D008832:Microchemistry; D010209:Paper; D012281:Rickettsia / Q000276:immunology; D012282:Rickettsia Infections / Q000097:blood / Q000175:diagnosis*
D000907:Antibodies, Bacterial