Myeloperoxidase activity of the large intestine in an equine model of acute colitis.
OBJECTIVE
To determine whether quantification of myeloperoxidase (MPO) activity could be a useful laboratory technique to detect granulocyte infiltration in equine intestinal tissues.


METHODS
Intestinal tissue (inflamed or healthy) collected from 16 age- and sex-matched Shetland Ponies.


METHODS
Intestinal tissue MPO activity was determined, and histologic assessment of adjacent specimens from healthy and inflamed intestine was done.


RESULTS
Intestinal tissue MPO activity and histopathologic score increased with time after castor oil challenge and peaked at 16 hours in an equine diarrhea model in which individual ponies provided their own control tissues.


CONCLUSIONS
Intestinal tissue inflammation scores correlated positively with tissue MPO activity in adjacent specimens.


CONCLUSIONS
Tissue MPO assay may be a useful laboratory tool to quantify intestinal mucosal inflammation in ponies.
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
R S
RS
McConnico
D
D
Weinstock
M E
ME
Poston
M C
MC
Roberts
D000818:Animals; D001560:Benzidines / Q000737:chemistry; D002368:Castor Oil / Q000009:adverse effects; D002432:Cecum / Q000473:pathology; D002863:Chromogenic Compounds / Q000737:chemistry; D017091:Colitis, Ischemic / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D003106:Colon / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006734:Horse Diseases / Q000503:physiopathology*; D006736:Horses; D007082:Ileum / Q000473:pathology; D007249:Inflammation / Q000662:veterinary; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology; D007420:Intestine, Large / Q000201:enzymology* / Q000473:pathology; D008297:Male; D009195:Peroxidase / Q000737:chemistry
D001560:Benzidines; D002863:Chromogenic Compounds; C021758:3,3',5,5'-tetramethylbenzidine; D002368:Castor Oil; D009195:Peroxidase