A mutational study of a Diels-Alderase catalytic antibody.
Site-directed mutagenesis was used to probe the mechanism of antibody 39-A11, which catalyzes the Diels-Alder reaction of substrate 1 and 2 to give cycloadduct 3. Mutations that lead to improved packing interactions with the diene afford an order of magnitude increase in catalytic activity.
Romesberg
F E
FE
Howard Hughes Medical Institute and the Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Schultz
P G
PG
D017151:Antibodies, Catalytic / Q000737:chemistry*; D018360:Crystallography, X-Ray; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed
D017151:Antibodies, Catalytic