Isolation of a novel microorganism, pestalotia heterocornis, producing paclitaxel
Pestalotia heterocornis was isolated from soil collected in yew forest and was shown to produce paclitaxel in semisynthetic liquid media. The presence of paclitaxel in the fungal extract was confirmed by FAB mass spectrometry and NMR spectroscopy. The maximum yield of paclitaxel was 31 &mgr;g per liter. Optimal paclitaxel production occurred after 5-7 days in a 20-liter scale fermentation at 23 degrees C. These results indicate that P. heterocornis is an excellent candidate for consideration in fermentation technology. Copyright 1999 John Wiley & Sons, Inc.
Biotechnology Team, KOLON Central Research Institute, 207-2, Mabuk-ri, Guseong-myun, Yongin City, Kyunggi-do, 449-910, Korea.
MJ
Noh
JG
Yang
KS
Kim
YM
Yoon
KA
Kang
HY
Han
SB
Shim
HJ
Park