Extracellular matrix gene expression in the left ventricular tissue of spontaneously hypertensive rats.
The aim of this study was to investigate the extracellular matrix gene expression in the hypertrophied left ventricular tissue of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats, at early and mature ages. Interestingly, with age, a marked increase (+85% and +187% at 25 and 30 weeks of age, respectively, p < 0.01, vs 5 weeks) in matrix metalloproteinase-1 (MMP-1) mRNA levels in SHR and a progressive decrease (-50%, -70%, -78%, -70% at 10, 15, 25 and 30 weeks, respectively, p < 0.01, vs 5 weeks) in WKY were seen. Moreover, mRNA levels were significantly lower in SHR at 5 weeks. The analysis of mRNA expression for the tissue inhibitor of metalloproteinase-1 (TIMP-1) showed a significant increase in WKY (+44% and +44%, vs 15 and 25 weeks, respectively, p < 0.05), whereas there were no significant changes in SHR with development. At 30 weeks TIMP-1 mRNA levels were significantly reduced in SHR. Temporal trends of procollagen alpha1(I) and procollagen alpha1(III) mRNA levels were similar in both strains, but lower levels for procollagen alpha1(III) were found in SHR at 5 and 30 weeks. Although no significant differences were measured between the strains, mRNA levels for fibronectin were found decreased in WKY and increased in SHR with age. The results of the present study suggest an altered balance between collagen deposition and collagen degradation with development in this model of left ventricular hypertrophy and hypertension.
Seccia
T M
TM
Department of Internal Medicine, University of Bari, Italy. a.pirrelli@dimo.uniba.it
Bettini
E
E
Vulpis
V
V
Quartaroli
M
M
Trist
D G
DG
Gaviraghi
G
G
Pirrelli
A
A
D000375:Aging / Q000502:physiology; D000818:Animals; D017364:Collagenases / Q000032:analysis / Q000235:genetics; D005109:Extracellular Matrix / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1; D011347:Procollagen / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D016326:Extracellular Matrix Proteins; D011347:Procollagen; D012333:RNA, Messenger; D017364:Collagenases; D020781:Matrix Metalloproteinase 1