A monoclonal antibody that recognizes the predicted tick-borne encephalitis virus E protein fusion sequence blocks fusion.
The fusion motif of tick-borne encephalitis virus E protein has been predicted to be located within its conserved region (98-120). Results are presented to demonstrate that non-neutralizing monoclonal antibody which recognizes a synthetic peptide corresponding to residues 98-113 of the E protein sequence can block the fusion of the virus particles with artificial membranes.
Volkova
T D
TD
Shimyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
Vorovitch
M F
MF
Ivanov
V T
VT
Timofeev
A V
AV
Volpina
O M
OM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology / Q000502:physiology; D017124:Conserved Sequence; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D007074:Immunoglobulin G; D008561:Membrane Fusion / Q000502:physiology*; D008567:Membranes, Artificial; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus