Presence of the proteinase-activated receptor-2 (PAR-2) in human brain tumor cells--trypsin- and SLIGRL-induced calcium response in primary cultured meningiomas.
This study focused on proteinase-activated receptor-2 (PAR-2) in primary cultured human meningioma cells. Stimulation of these cells with the serine proteinase trypsin resulted in a dose-dependent transient calcium response. Since the specific PAR-2 agonist peptide SLIGRL also induced [Ca2+]i mobilization in human meningioma cells and successive application of SLIGRL and trypsin elicited no new calcium signal we conclude that trypsin-induced calcium signaling is mediated by PAR-2 in human meningioma cells. To our knowledge, this is the first report describing functional PAR-2-type receptors in human brain tumor cells.
Kaufmann
R
R
Research Group Pharmacological Hemostaseology, Medical Faculty at the Friedrich Schiller-University Jena, Germany. rol@leech.mpg.uni-jena.de
Patt
S
S
Zieger
M
M
Kraft
R
R
Nowak
G
G
D001932:Brain Neoplasms / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D008579:Meningioma / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D044464:Receptor, PAR-2; D018179:Receptors, Thrombin / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D009842:Oligopeptides; D044464:Receptor, PAR-2; D018179:Receptors, Thrombin; C099570:seryl-leucyl-isoleucyl-glycyl-arginyl-leucine; D014357:Trypsin; D002118:Calcium