In ovo vaccination of specific-pathogen-free chickens with vaccines containing multiple agents.
We used in ovo technology to protect chickens against multiple diseases by inoculating vaccines containing mixtures of live viral agents. A single in ovo injection of a vaccine containing serotypes 1, 2, and 3 of Marek's disease virus (MDV), a vaccine strain of serotype 1 infectious bursal disease virus (IBDV), and recombinant fowl pox vaccine with HN and F genes of Newcastle disease virus (rFP-NDV) induced protection against virulent MDV, IBDV, Newcastle disease virus, and fowl poxvirus. The multiple-agent vaccine induced specific antibodies against the viral agents present in the mixture and did not adversely affect the survival of hatched chickens. Inoculation of a vaccine containing serotypes 1, 2, and 3 of MDV and IBDV did not affect hatchability of eggs, although the addition of rFP-NDV to the mixture reduced hatchability by 23%-26%. In ovo vaccination with a vaccine containing MDV and IBDV vaccine viruses did not exacerbate the inhibitory effect of individual viral agents on humoral and cellular immune competence.
Gagic
M
M
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
St Hill
C A
CA
Sharma
J M
JM
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002645:Chickens / Q000276:immunology*; D005587:Fowlpox virus / Q000276:immunology*; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D010063:Ovum / Q000821:virology*; D011201:Poultry Diseases / Q000517:prevention & control*; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D017778:Vaccines, Combined; D014765:Viral Vaccines