Imaging time-of-flight secondary ion mass spectrometry of solid-phase peptide syntheses.
Imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) of solid-phase peptide syntheses carried out by the Merrifield and Sheppard strategies is described. Mixtures of resin beads mixed at random from batch syntheses or obtained in combinatorial chemistry by the mix and split technique, where each bead is functionalized by a unique peptide, were analyzed directly without any chemical cleavage of the growing chains to assess the nature of the growing structure on any bead of the mixture without its isolation.
Aubagnac
J L
JL
UMR 5810, Laboratoire des Aminoacides, Peptides et Protéines, Université Montpellier II, Place E. Bataillon, 34095 Montpellier Cédex 05, France. aubagnac@univ-montp2.fr
Enjalbal
C
C
Drouot
C
C
Combarieu
R
R
Martinez
J
J
D000596:Amino Acids / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry; D018629:Spectrometry, Mass, Secondary Ion
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; D012117:Resins, Synthetic