Dally cooperates with Drosophila Frizzled 2 to transduce Wingless signalling.
The Drosophila wingless gene (wg) encodes a protein of the Wnt family and is a critical regulator in many developmental processes. Biochemical studies have indicated that heparan sulphate proteoglycans, consisting of a protein core to which heparan sulphate glycosaminoglycans are attached, are important for Wg function. Here we show that, consistent with these findings, the Drosophila gene sulfateless (sfl), which encodes a homologue of vertebrate heparan sulphate N-deacetylase/N-sulphotransferase (an enzyme needed for the modification of heparan sulphate) is essential for Wg signalling. We have identified the product of division abnormally delayed (dally), a glycosyl-phosphatidyl inositol (GPI)-linked glypican, as a heparan sulphate proteoglycan molecule involved in Wg signalling. Our results indicate that Dally may act as a co-receptor for Wg, and that Dally, together with Drosophila Frizzled 2, modulates both short- and long-range activities of Wg.
Lin
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Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Perrimon
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N
D000581:Amidohydrolases / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins; D005260:Female; D051157:Frizzled Receptors; D017344:Genes, Insect; D006493:Heparin / Q000031:analogs & derivatives / Q000502:physiology; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology; D008562:Membrane Glycoproteins / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D011509:Proteoglycans / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015238:Sulfotransferases / Q000235:genetics / Q000502:physiology; D014921:Wings, Animal / Q000196:embryology; D051155:Wnt1 Protein
D029721:Drosophila Proteins; D051157:Frizzled Receptors; D019476:Insect Proteins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D011518:Proto-Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter; D051155:Wnt1 Protein; C098128:dally protein, Drosophila; C101703:fz2 protein, Drosophila; C036090:heparin proteoglycan; C074622:wg protein, Drosophila; D006493:Heparin; D015238:Sulfotransferases; C021350:heparitin sulfotransferase; C500949:sfl protein, Drosophila; D000581:Amidohydrolases