Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins.
The pathogenic fungus Sporothrix schenckii is the causative agent of sporotrichosis. This subcutaneous mycosis may disseminate in immunocompromised individuals and also affect several internal organs and tissues, most commonly the bone, joints and lung. Since adhesion is the first step involved with the dissemination of pathogens in the host, we have studied the interaction between S. schenckii and several extracellular matrix (ECM) proteins. The binding of two morphological phases of S. schenckii, yeast cells and conidia, to immobilized type II collagen, laminin, fibronectin, fibrinogen and thrombospondin was investigated. Poly (2-hydroxyethyl methacrylate) (poly-HEMA) was used as the negative control. Cell adhesion was assessed by ELISA with a rabbit anti-S. schenckii antiserum. The results indicate that both morphological phases of this fungus can bind significantly to type II collagen, fibronectin and laminin in comparison to the binding observed with BSA (used as blocking agent). The adhesion rate observed with the ECM proteins (type II collagen, fibronectin and laminin) was statistically significant (P < 0.05) when compared to the adhesion obtained with BSA. No significant binding of conidia was observed to either fibrinogen or thrombospondin, but yeast cells did bind to the fibrinogen. Our results indicate that S. schenckii can bind to fibronectin, laminin and type II collagen and also show differences in binding capacity according to the morphological form of the fungus.
Departamento de Biologia Celular e Genética, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Brasil.
O C
OC
Lima
C C
CC
Figueiredo
B A
BA
Pereira
M G
MG
Coelho
V
V
Morandi
L M
LM
Lopes-Bezerra
D002448:Cell Adhesion; D003094:Collagen / Q000302:isolation & purification; D016326:Extracellular Matrix Proteins / Q000378:metabolism* / Q000502:physiology; D005353:Fibronectins; D007797:Laminin; D013175:Sporothrix / Q000472:pathogenicity* / Q000502:physiology; D013174:Sporotrichosis / Q000382:microbiology; D019699:Thrombospondins
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D019699:Thrombospondins; D003094:Collagen