[Characteristics of carbohydrate metabolism of R-, S- i M-dissociants of Pseudomonas aeruginosa].
R and S dissociants of the hydrocarbon-oxidizing strain Pseudomonas aeruginosa K-2 differed but little in their growth in a minimal defined medium with glucose as the source of carbon and energy. At the same time, the number of cells of M dissociant in the late exponential phase was five orders of magnitude less than that of R and S dissociants. The growth of M dissociant was accompanied by the accumulation of formate in the culture liquid and a concurrent decrease in pH. All three dissociants contained the key enzymes of the Entner-Doudoroff pathway of glucose oxidation; however, the activities of these enzymes, especially 6-phosphogluconate dehydrogenase, were low in M dissociant. Conversely, the activity of formate dehydrogenase in cells of M dissociant was higher than in other dissociants. The activity of 6-phosphogluconate dehydrogenase, a key enzyme of the pentosephosphate pathway of glucose oxidation, was detected only in S dissociant. The peculiarities of the carbohydrate metabolism of M dissociant are probably responsible for its poor growth on glucose and determine the more pronounced anaerobic type of its metabolism.
Moscow State University, Russia.
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Mil'ko
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Krasil'nikova
D050260:Carbohydrate Metabolism; D003470:Culture Media; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media
Osobennosti uglevodnogo metabolizma R-, S- i M-dissotsiantov Pseudomonas aeruginosa.