Leginon: a system for fully automated acquisition of 1000 electron micrographs a day.
We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.
Potter
C S
CS
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign 61801, USA. cpotter@uiuc.edu
Chu
H
H
Frey
B
B
Green
C
C
Kisseberth
N
N
Madden
T J
TJ
Miller
K L
KL
Nahrstedt
K
K
Pulokas
J
J
Reilein
A
A
Tcheng
D
D
Weber
D
D
Carragher
B
B
D001331:Automation / Q000379:methods*; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*