Bacterial expression of the major antigenic regions of porcine rotavirus VP7 induces a neutralizing immune response in mice.
The outer capsid protein of rotavirus, VP7, is a major neutralization antigen. A chimeric protein comprising Escherichia coli (E. coli) outer membrane protein A (OmpA) and part of porcine rotavirus VP7 containing all three antigenic regions (217 amino acids) was expressed in Salmonella and E. coli as an outer-membrane associated protein. Mice immunized intraperitoneally or orally, respectively, with live E. coli or Salmonella cells expressing this chimeric protein produced antibodies against native VP7 as determined by enzyme-linked immunosorbent assays and neutralization tests. This indicates that the VP7 fragment from a porcine rotavirus which is antigenically similar to human rotavirus serotype 3, when expressed in bacteria as a chimeric protein, can form a structure resembling its native form at least in some of the major neutralization domains. These results indicate that the use of a live bacterial vector expressing rotavirus VP7 may represent a strategy for the development of vaccines against rotavirus-induced diarrhoea in infants.
Department of Microbiology, The University of Sydney, NSW, Australia.
L
L
Wang
J A
JA
Huang
H S
HS
Nagesha
S C
SC
Smith
A
A
Phelps
I
I
Holmes
J C
JC
Martyn
P J
PJ
Coloe
P R
PR
Reeves
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology*; D012475:Salmonella / Q000235:genetics; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D001425:Bacterial Outer Membrane Proteins; D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; C050779:VP7 protein, Rotavirus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C078167:OMPA outer membrane proteins