Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines.
Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed.
van Schothorst
E M
EM
Laboratory for Experimental Patho-Oncology, Pathology/Daniel den Hoed Cancer Center, Josephine Nefkens Institute, University Hospital Rotterdam, The Netherlands.
Mohkamsing
S
S
van Gurp
R J
RJ
Oosterhuis
J W
JW
van der Saag
P T
PT
Looijenga
L H
LH
D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000074018:B-Cell CLL-Lymphoma 10 Protein; D001483:Base Sequence; D004273:DNA, Neoplasm; D005091:Exons; D018237:Germinoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D013736:Testicular Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins