Semen specific gamma-glutamyltransferase carries ABH antigens: a sandwich ELISA for simultaneous semen detection and its ABO blood typing.
Semen type of gamma-glutamyltransferase (gamma-GTP) is different from the membrane bound type of the enzyme in both biochemical and immunological properties, and consists of two subunits (150 and 95 kDa). We found that anti-ABH antibodies recognize a 150-kDa subunit of seminal gamma-GTP by Western blot and immunoprecipitation analyses. Using SG2, one of anti-semen specific gamma-GTP monoclonal antibodies which we had produced, and anti-ABH antibodies, we established a sandwich ELISA for identifying human seminal gamma-GTP and its ABO type simultaneously. This sandwich ELISA allows ABO typing of highly diluted semen. The dilutions for ABO typing were 10(5) times for A or O, and 10(4) times for B. Furthermore, ABO typing of semen was successfully performed by this ELISA, even in the mixed presence of vaginal fluid, saliva and blood. Thus, seminal gamma-GTP carries ABH antigens and the sandwich ELISA with SG2 and anti-ABH antibodies enables ABO typing of semen. The sandwich ELISA is extremely useful for ABO typing originated from semen in the mixture of biological fluids.
Abe
S
S
Department of Legal Medicine, Fukushima Medical University, School of Medicine, Japan. sumiko@cc.fmu.ac.jp
Gunji
H
H
Kunii
S
S
Kuraya
M
M
Fujita
T
T
Hiraiwa
K
K
D000017:ABO Blood-Group System / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000379:methods; D001788:Blood Grouping and Crossmatching; D001826:Body Fluids / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D015151:Immunoblotting; D008297:Male; D011233:Precipitin Tests; D012463:Saliva / Q000737:chemistry; D012661:Semen / Q000201:enzymology*; D012680:Sensitivity and Specificity; D005723:gamma-Glutamyltransferase / Q000276:immunology*
D000017:ABO Blood-Group System; D000941:Antigens; D005723:gamma-Glutamyltransferase