Physical and meiotic mapping of the region of human chromosome 4q11-q13 encompassing the vitamin D binding protein DBP/Gc-globulin and albumin multigene cluster.
The vitamin D binding protein/Gc-globulin (DBP) gene is a member of a multigene cluster that includes albumin (ALB), alpha-fetoprotein (AFP), and alpha-albumin/afamin (AFM). All four genes have structural and functional similarities and map to the same chromosomal regions in humans (4q11-q13), mice, and rats. An accurate physical map of the region encompassing these genes is a prerequisite for study of their respective transcriptional regulation and identification of potential shared regulatory elements. By refining the physical and meiotic maps of the 4q11-q13 region and creating a local PAC contig, the order and transcriptional orientations of these four genes were determined to be centromere-3'-DBP-5'-5'-ALB-3'-5'-AFP-3'-5'-AFM3'-telomere. The ancestral DBP gene was separated from the ALB gene by >1.5 Mb. This organization and spacing establishes a foundation for ongoing functional studies in this region.
Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA.
Y H
YH
Song
A K
AK
Naumova
S A
SA
Liebhaber
N E
NE
Cooke
D000418:Albumins / Q000235:genetics*; D000818:Animals; D002352:Carrier Proteins; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006023:Glycoproteins; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D005810:Multigene Family / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D051381:Rats; D012709:Serum Albumin / Q000235:genetics; D000075462:Serum Albumin, Human; D014158:Transcription, Genetic / Q000235:genetics; D014809:Vitamin D-Binding Protein / Q000235:genetics*; D000509:alpha-Fetoproteins / Q000235:genetics
C088246:AFM protein, human; D000418:Albumins; D002352:Carrier Proteins; D005819:Genetic Markers; D006023:Glycoproteins; D012709:Serum Albumin; D014809:Vitamin D-Binding Protein; D000509:alpha-Fetoproteins; D000075462:Serum Albumin, Human