Glutathione-dependent ascorbate recycling activity of rat serum albumin.
An efficient regeneration of vitamin C (ascorbate) from its oxidized byproduct, dehydroascorbate (DHAA), is necessary to maintain sufficient tissue levels of the reduced form of the vitamin. Additionally, the recycling may be more significant in mammals, such as guinea pigs and humans, who have lost the ability to synthesize ascorbate de novo, than it is in most other mammals who have retained the ability to synthesize the vitamin from glucose. Both a chemical and an enzymatic reduction of DHAA to ascorbate have been proposed. Several reports have appeared in which proteins, including thioltransferase, protein disulfide isomerase, and 3-alpha-hydroxysteroid dehydrogenase, characterized for other activities have been identified as having DHAA reductase activity in vitro. Whether these previously characterized proteins catalyze the reduction of DHAA in vivo is unclear. In the present study, a 66 kD protein was purified strictly on the basis of its DHAA-reductase activity and was identified as rat serum albumin. The protein was further characterized and results support the suggestion that serum albumin acts as an antioxidant and exerts a significant glutathione-dependent DHAA-reductase activity that may be important in the physiologic recycling of ascorbic acid.
Department of Physiology, University of North Dakota School of Medicine and Health Sciences, Grand Forks.
J G
JG
Vethanayagam
E H
EH
Green
R C
RC
Rose
A M
AM
Bode
D000595:Amino Acid Sequence; D000818:Animals; D000937:Antigen-Antibody Reactions; D001205:Ascorbic Acid / Q000378:metabolism*; D003683:Dehydroascorbic Acid / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism*
D012709:Serum Albumin; D005978:Glutathione; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid