Variability of cytotoxicity and leaching of substances from four light-curing pit and fissure sealants.
It was the aim of our study to investigate the composition and cytotoxicity of aqueous extracts of four light-curing pit and fissure sealants. Water extracts were analyzed by gas chromatography/mass spectrometry (GC/MS), and relative quantities of identified compounds were compared by means of an internal caffeine standard [%CF]. Cytotoxic effects due to medium extracts were determined by means of permanent 3T3 fibroblasts. All light-curing pit and fissure sealants segregated different ingredients into water, such as co-monomers (mainly ethylene glycol compounds) and initiating substances (e.g., camphorquinone). Bisphenol-A, however, which is easily detected by GC/MS, was not found in any of the analyzed eluates. The extracts of three sealants inhibited monolayer growth only moderately whereas the eluate of one product inhibited cell proliferation significantly. In the extracts of this sealant high quantities [%CF] of the co-monomer TEGDMA were detected. Our results indicate that light-curing pit and fissure sealants release substances into aqueous media that may induce cytotoxic effects. However, no concerns about potential estrogenic effects of Bisphenol-A are supported by our results.
Department of Conservative Dentistry & Periodontology, Medical University Hannover, D-30623 Hannover, Germany.
W
W
Geurtsen
W
W
Spahl
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G
Leyhausen
D016475:3T3 Cells; D000818:Animals; D002110:Caffeine / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects*; D005026:Ethylene Glycols / Q000737:chemistry* / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry; D051379:Mice; D010895:Pit and Fissure Sealants / Q000737:chemistry* / Q000633:toxicity*; D013329:Structure-Activity Relationship
D005026:Ethylene Glycols; D010895:Pit and Fissure Sealants; D002110:Caffeine