E4ORF3 requirement for achieving long-term transgene expression from the cytomegalovirus promoter in adenovirus vectors.
Analysis of transgene expression under the control of the cytomegalovirus (CMV) promoter from adenovirus vectors in which the E4 region was modified indicated that E4ORF3 is required for long-term expression in the murine lung. CMV promoter truncation led to the persistence of expression in the absence of E4, thus eliminating the ORF3 requirement.
Armentano
D
D
Genzyme Corporation, Framingham, Massachusetts 01701-9322, USA. darmentano@genzyme.com
Smith
M P
MP
Sookdeo
C C
CC
Zabner
J
J
Perricone
M A
MA
St George
J A
JA
Wadsworth
S C
SC
Gregory
R J
RJ
D017504:Adenovirus E4 Proteins / Q000235:genetics*; D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001665:Binding Sites; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D013997:Time Factors; D019076:Transgenes
D017504:Adenovirus E4 Proteins; C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator