Gas chromatographic-mass spectrometric determination of total homocysteine in human plasma by stable isotope dilution: method and clinical applications.
The detection and quantitation of slight increases of plasma homocysteine levels is of growing interest. This has prompted us to develop a highly sensitive and accurate capillary gas chromatography-mass spectrometry (GC-MS) method. The method proved to be highly sensitive (DL=0.17 micromol/l) with between- and within-run precision less than 6% and 7%, respectively. Reference values of plasma total homocysteine have been determined for men (n=39) and women (n=36), showing a significant difference (P=0.003) between gender. Preliminary results in cerebrovascular accidents and in venous thrombosis are presented.
Laboratoire de Biochimie C, CHU de Grenoble, France.
V
V
Ducros
D
D
Schmitt
G
G
Pernod
H
H
Faure
B
B
Polack
A
A
Favier
D000328:Adult; D002138:Calibration; D016022:Case-Control Studies; D002561:Cerebrovascular Disorders / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007554:Isotopes; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D020246:Venous Thrombosis / Q000097:blood
D007554:Isotopes; D006710:Homocysteine