Experimental model for optimization of hydrophobic interaction: chromatographic purification of glucagon.
The chromatographic behavior of the components of glucagon-containing material from bovine pancreas by hydrophobic interaction on Amberlite XAD-7 was investigated. The effect of initial ionic strength and pH of the mobile phase on selectivity was quantitatively determined. The data, obtained from 56 experiments, were used for modeling and the corresponding management of the purification process. The purified glucagon is of very high quality: total impurities are not more than 1.7% as determined by analytical HPLC. The biological activity is 1.08 IU/mg, calculated for dry substance.
Chemical Pharmaceutical Research Institute, NIHFI, Sofia, Bulgaria.
N
N
Dimov
M
M
Angelova
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005934:Glucagon / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D009994:Osmolar Concentration; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D005934:Glucagon