Enzymatic capacities for beta-oxidation of fatty fuels are low in the gill of teleost fishes despite presence of fatty acid-binding protein.
A variety of circulating fuels can support the work of the teleost gill. Previous work indicates, however, that unlike other aerobic tissues from teleosts, the gill may have a limited capacity to oxidize fatty fuels. We determined capacities for catabolism of carbohydrate, fatty acids, and amino acids in four species of temperate marine or euryhaline teleosts representing distinct lineages. In addition, we assessed the capacity for fatty acid oxidation in the gill from an Antarctic species. Activities of rate-limiting or regulatory enzymes from pathways of energy metabolism were measured at physiological temperatures (15 degrees or 1 degrees C). In the temperate species, ATP yields from glucose are 3- to 30-fold greater (varying with species) than ATP yields from a monounsaturated fatty acid, while ATP generation from glutamate is 2-50 times greater than similar capacities for the lipid fuel. Like the temperate species, capacity for beta-oxidation of fatty acids is limited in the Antarctic species. A positive linear correlation between activities of citrate synthase (central pathway of oxidative metabolism) and hexokinase (glycolysis) adds further support to the hypothesis that glucose is a preferred metabolic fuel in gill. Our results also demonstrate that fatty acid-binding protein is present in the gill of teleost fishes. It is likely that this protein plays a more important role facilitating anabolic pathways in lipid metabolism rather than fatty acid oxidation in the gill of teleost fishes.
Department of Biological Sciences, Ohio University, Athens, Ohio 45701, USA. crockett@ohiou.edu
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Crockett
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Londraville
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Wilkes
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Popesco
D000255:Adenosine Triphosphate / Q000378:metabolism; D000811:Anguilla / Q000502:physiology*; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D004734:Energy Metabolism; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005880:Gills / Q000201:enzymology* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D018996:Myelin P2 Protein / Q000378:metabolism*; D009363:Neoplasm Proteins; D010084:Oxidation-Reduction; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism; D013379:Substrate Specificity
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D000255:Adenosine Triphosphate; D002334:Carnitine O-Palmitoyltransferase; D010170:Palmitoyl-CoA Hydrolase; D005947:Glucose