A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis.
A multisubunit complex, called cohesin, containing Smc1p, Smc3p, Scc1p, and Scc3p, is required for sister chromatid cohesion in mitotic cells. We show here that Smc3p and a meiotic version of Scc1p called Rec8p are required for cohesion between sister chromatids, for formation of axial elements, for reciprocal recombination, and for preventing hyperresection of double-strand breaks during meiosis. Both Rec8p and Smc3p colocalize with chromosome cores independently of synapsis during prophase I and largely disappear from chromosome arms after pachytene but persist in the neighborhood of centromeres until the onset of anaphase II. The eukaryotic cell's cohesion apparatus is required both for the repair of recombinogenic lesions and for chromosome segregation and therefore appears to lie at the heart of the meiotic process.
Klein
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F
Institute of Botany, University of Vienna, Austria. fklein@s1.botanik.univie.ac.at
Mahr
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Galova
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Buonomo
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SB
Michaelis
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C
Nairz
K
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Nasmyth
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K
D000705:Anaphase; D000818:Animals; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002503:Centromere / Q000235:genetics / Q000648:ultrastructure; D011508:Chondroitin Sulfate Proteoglycans; D002842:Chromatids / Q000235:genetics* / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D008540:Meiosis; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins; D010802:Phylogeny; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1
D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1; C490529:MCD1 protein, S cerevisiae; C120372:SMC3 protein, S cerevisiae; C492318:SMC3 protein, human