[The optimization of a method for determining RNAse activity by using high-polymer RNA].
Different methods for precipitation of free nucleotides are compared with the aim of improving the method for estimating RNAse activity. The precipitators were 0.75% uranyl acetate in 0.25% HClO4, 96% ethanol with MgCl2 in different concentrations, pure ethanol, and chlorine acid. Measurements of RNAse activity by means of 0.02M MgCl2 in 96% ethanol whose volume is equal to the reaction mixture were the optimal.
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Kolpakov
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ON
Il'inskaia
D001407:Bacillus / Q000201:enzymology; D007202:Indicators and Reagents; D008970:Molecular Weight; D012331:RNA, Fungal / Q000378:metabolism*; D012260:Ribonucleases / Q000032:analysis*; D012441:Saccharomyces cerevisiae; D013379:Substrate Specificity; D013997:Time Factors
D007202:Indicators and Reagents; D012331:RNA, Fungal; D012260:Ribonucleases
Optimizatsiia metoda opredeleniia aktivnosti RNKazy s ispol'zovaniem vysokopolimernoĭ RNK.