Evaluation of teflon-coated intraocular lenses in an organ culture method.
An amorphous and transparent form of Teflon is proposed as a coating of polymethylmethacrylate (PMMA) intraocular lenses (IOLs), rendering them highly hydrophobic. We used an organ culture method to evaluate cell adhesion, proliferation, and migration on Teflon-coated IOLs. Corneal explants from 14-day-old chicken embryos were placed on a semisolid culture medium and covered with uncoated PMMA (n = 36) and Teflon-coated PMMA (n = 36) IOLs and two controls, Thermanox (n = 84) and latex (n = 36). After incubation (7 days at 37 degrees C), a digital imaging system was used to measure the areas of the cell migration layers on the materials. The cells were then removed with tripsin-ethylenediaminetetraacetic acid and the cells detached at times up to 75 min were counted (Coulter(R) Multisizer System). The values were used to construct a cell disconnecting curve for each material. The areas of cell migration layers on uncoated and Teflon-coated IOLs were significantly different (p <.05). Cell disconnecting curves demonstrated that cells adhered less strongly to Teflon-coated IOLs than to the other materials. This organ culture method demonstrated that the coating of PMMA IOLs with Teflon AF(R) is correlated with antiadhesive and antiproliferative properties.
Werner
L
L
Department of Ophthalmology, Hôtel-Dieu Hospital, and Laboratoire de Recherche sur les Therapeutiques Substitutives en Ophtalmologie (EA 2395, CRI 9808), 1, place du Parvis-Notre-Dame, F-75181, Paris Cedex 04, France.
Legeais
J M
JM
Nagel
M D
MD
Renard
G
G
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002642:Chick Embryo; D003315:Cornea / Q000166:cytology; D005069:Evaluation Studies as Topic; D019654:Lens Implantation, Intraocular; D008422:Materials Testing; D009924:Organ Culture Techniques; D019904:Polymethyl Methacrylate; D011138:Polytetrafluoroethylene; D013499:Surface Properties
D011138:Polytetrafluoroethylene; D019904:Polymethyl Methacrylate