Production of singlet oxygen by eosinophils activated in vitro by C5a and leukotriene B4.
Using the trans-methoxyvinylpyrene analogues of benzo[a]pyrene-7,8-dihydrodiol (MVP) as a singlet oxygen ((1)O2) chemiluminescence probe, we have demonstrated that guinea pig eosinophils release (1)O2 when activated with the physiological agonists C5a and leukotriene B4. This release, which occurs at agonist concentrations as low as 10(-7) M, occurs more rapidly than activation with phorbol ester (10(-6) M), is similar in level, but is more transitory. In addition, the release of (1)O2 occurs in the absence of added bromide ions and represents, we propose, an important feature of eosinophil-mediated inflammatory damage.
Department of Pharmacology, Instituto de Ciências Biologicas, Universidade Federal de Minas Gerais, Brazil.
M M
MM
Teixeira
F Q
FQ
Cunha
A
A
Noronha-Dutra
J
J
Hothersall
D000166:Acridines; D000818:Animals; D015936:Complement C5a / Q000494:pharmacology*; D004101:Dihydroxydihydrobenzopyrenes / Q000737:chemistry; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006168:Guinea Pigs; D017665:Hydroxyl Radical / Q000378:metabolism; D007975:Leukotriene B4 / Q000494:pharmacology*; D008163:Luminescent Measurements; D015335:Molecular Probes; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D026082:Singlet Oxygen; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000166:Acridines; D004101:Dihydroxydihydrobenzopyrenes; D016166:Free Radical Scavengers; D015335:Molecular Probes; D009566:Nitrates; D013481:Superoxides; C017228:benzo(a)pyrene 7,8-dihydrodiol; D026082:Singlet Oxygen; D007975:Leukotriene B4; C033472:10,10'-dimethyl-9,9'-biacridinium; C039642:peroxynitric acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D015936:Complement C5a; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen