A substrate combinatorial array for caspases.
An efficient strategy for the synthesis of a tetrapeptidyl substrate combinatorial array directed toward the caspases is described. Testing of this array with caspases 1 and 4 gave substrate hydrolytic profiles characteristic of each caspase, and permitted the identification of efficiently processed substrates. A comparison of this approach to that using a positional scanning library is presented.
Department of Medicinal Chemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, USA.
D
D
Lee
J L
JL
Adams
M
M
Brandt
W E
WE
DeWolf
P M
PM
Keller
M A
MA
Levy
D020170:Caspase 1 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism*; D053471:Caspases, Initiator; D002384:Catalysis; D007700:Kinetics; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D013379:Substrate Specificity
D009842:Oligopeptides; C093516:CASP4 protein, human; D020169:Caspases; D053471:Caspases, Initiator; D020170:Caspase 1