Expression and cellular localization of Atrab28 during arabidopsis embryogenesis.
The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)+ stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies.
Departament de Genètica Molecular, Centre d'Investigació i Desenvolupament, C.S.I.C., Barcelona, Spain.
C
C
Arenas-Mena
M
M
Raynal
A
A
Borrell
F
F
Varoquaux
M C
MC
Cutanda
R A
RA
Stacy
M
M
Pagès
M
M
Delseny
F A
FA
Culiáñez-Macià
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000196:embryology / Q000235:genetics*; D029681:Arabidopsis Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D005810:Multigene Family; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors; D015533:Transcriptional Activation; D014867:Water / Q000494:pharmacology
C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; C120342:Atrab28 protein, Arabidopsis; D018076:DNA, Complementary; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C066246:late embryogenesis abundant protein, plant; D014867:Water; D000040:Abscisic Acid