Reassessment of the calibration constant for the IAsys biosensor.
A magnitude of 50 are s ng-1 mm2 has been determined for the calibration constant relating biosensor response to the amount of protein bound to the sensor surface of an IAsys cuvette. These studies entailed enzymatic assessment of the extent of lactate dehydrogenase depletion in the liquid phase arising from enzyme binding to a carboxymethyldextran-coated sensor surface, and also estimation of a maximum biosensor response for the electrostatic interaction of ovalbumin with an aminosilane-coated sensor surface. The latter results required correction for contributions to biosensor response resulting from changes in the refractive index of the liquid phase effected by high protein concentrations.
Hall
D R
DR
Department of Biochemistry, University of Queensland, Brisbane, Australia.
Winzor
D J
DJ
D015374:Biosensing Techniques; D002138:Calibration; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010047:Ovalbumin / Q000737:chemistry / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D055672:Static Electricity; D013816:Thermodynamics
D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D010047:Ovalbumin; D007770:L-Lactate Dehydrogenase