A complete set of repeat-depleted, PCR-amplifiable, human chromosome-specific painting probes.
We describe the generation of a complete set of human chromosome-specific painting probes depleted in repetitive sequences. These probes yield highly specific signals when hybridized without the addition of a blocking agent, such as Cot-1 DNA, and without probe preannealing prior to hybridization. Fluorescent intensities and signal-to-background ratios for these probes are comparable to those of untreated probes hybridized with Cot-1 DNA. We demonstrate the suitability of these probes for applications with very complex probe sets, such as multiplex-FISH.
Bolzer
A
A
Institut für Anthropologie and Humangenetik, LMU München, Germany.
Craig
J M
JM
Cremer
T
T
Speicher
M R
MR
D001667:Binding, Competitive; D020223:Chromosome Painting / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015342:DNA Probes / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004247:DNA