Cellular uptake of liposomes targeted to intercellular adhesion molecule-1 (ICAM-1) on bronchial epithelial cells.
Previously, it was demonstrated that immunoliposomes, bearing anti-intercellular adhesion molecule-1 (ICAM-1) antibodies (mAb F10.2), can specifically bind to different cell types expressing ICAM-1. In this study, we have quantified the amount of immunoliposomes binding to IFN-gamma activated human bronchial epithelial cells (BEAS-2B) in vitro and studied the subsequent fate of cell-bound anti-ICAM-1 immunoliposomes. We demonstrate that binding of the immunoliposomes to the epithelial cells depends on the liposome concentration used. After binding to the cell surface, the anti-ICAM-1 immunoliposomes are rapidly internalised by the epithelial cells. Sixty percent of cell-bound immunoliposomes were internalised by the epithelial cells within 1 h of incubation at 37 degrees C. The results indicate that ICAM-1 targeted immunoliposomes may be used as carriers for the intracellular delivery of anti-inflammatory drugs to sites of inflammation characterised by an increased expression of ICAM-1.
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80.082, 3508 TB, Utrecht, The Netherlands. e.mastrobattista@pharm.uu.nl
E
E
Mastrobattista
G
G
Storm
L
L
van Bloois
R
R
Reszka
P G
PG
Bloemen
D J
DJ
Crommelin
P A
PA
Henricks
D000911:Antibodies, Monoclonal / Q000276:immunology; D001980:Bronchi / Q000276:immunology*; D002448:Cell Adhesion; D002460:Cell Line; D004337:Drug Carriers; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism; D005452:Fluoresceins; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology* / Q000378:metabolism; D007371:Interferon-gamma; D008081:Liposomes / Q000276:immunology*; D018613:Microscopy, Confocal; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D005452:Fluoresceins; D008081:Liposomes; C083098:calcein green; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma