Synthesis of FinP RNA by plasmids F and pSLT is regulated by DNA adenine methylation.
DNA adenine methylase mutants of Salmonella typhimurium contain reduced amounts of FinP, an antisense RNA encoded by the virulence plasmid pSLT. Lowered FinP levels are detected in both Dam- FinO+ and Dam- FinO- backgrounds, suggesting that Dam methylation regulates FinP production rather than FinP half-life. Reduced amounts of F-encoded FinP RNA are likewise found in Dam- mutants of Escherichia coli. A consequence of FinP RNA scarcity in the absence of DNA adenine methylation is that Dam- mutants of both S. typhimurium and E. coli show elevated levels of F plasmid transfer. Inhibition of F fertility by the S. typhimurium virulence plasmid is also impaired in a Dam- background.
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
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D000225:Adenine / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D019175:DNA Methylation; D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005144:F Factor; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010641:Phenotype; D020161:Physical Chromosome Mapping; D010957:Plasmids; D016372:RNA, Antisense / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins; D012486:Salmonella typhimurium / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014169:Transformation, Bacterial; D014774:Virulence; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D016372:RNA, Antisense; D011993:Recombinant Fusion Proteins; C082576:traB protein, Bacteria; D001616:beta-Galactosidase; D000225:Adenine