A simple and accurate method for generating co-dominant markers: an application of conformation-sensitive gel electrophoresis to linkage analysis in the silkworm.
A simple and sensitive method for linkage analysis is described, which is based on conformation-sensitive gel electrophoresis (CSGE). Using urea-containing agarose gels or a commercially available polyacrylamide-derived matrix, 13 polymorphic markers were newly identified for known genes of the silkworm, Bombyx mori, which had been scored as monomorphic by PCR-RFLP analysis. This method for detecting polymorphisms is quite sensitive, and can be performed with inexpensive reagents and apparatus that is available in most molecular biology laboratories.
Yasukochi
Y
Y
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan. yaskoch@nises.affrc.go.jp
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D019992:Databases as Topic; D004586:Electrophoresis / Q000379:methods; D005260:Female; D005799:Genes, Dominant; D008040:Genetic Linkage; D005819:Genetic Markers; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers; D019476:Insect Proteins; D004247:DNA