Characterization of the human laminin beta2 chain locus (LAMB2): linkage to a gene containing a nonprocessed, transcribed LAMB2-like pseudogene (LAMB2L) and to the gene encoding glutaminyl tRNA synthetase (QARS).
The laminin beta2 chain is an important constituent of certain kidney and muscle basement membranes. We have generated a detailed physical map of a 110-kb genomic DNA segment surrounding the human laminin beta2 chain gene (LAMB2) on chromosome 3p21.3-->p21.2, a region paralogous with the chromosome 7q22-->q31 region that contains the laminin beta1 chain gene locus (LAMB1). Several CpG islands and a novel polymorphic microsatellite marker (D3S4594) were identified. The 3' end of LAMB2 lies 16 kb from the 5' end of the glutaminyl tRNA synthetase gene (QARS). About 20 kb upstream of LAMB2 we found a gene encoding a transcribed, non-processed LAMB2-like pseudogene (LAMB2L). The sequence of 1.75 kb of genomic DNA at the 3' end of LAMB2L was similar to exons 8-12 of the laminin beta2 chain gene. The LAMB2L-LAMB2-QARS cluster lies telomeric to the gene encoding the laminin-binding protein dystroglycan (DAG1).
Institute of Molecular Pathology, University of Copenhagen, Copenhagen, Denmark.
M E
ME
Durkin
A C
AC
Jäger
T S
TS
Khurana
F C
FC
Nielsen
R
R
Albrechtsen
U M
UM
Wewer
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D003001:Cloning, Molecular; D018899:CpG Islands / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D049030:Dystroglycans; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D007438:Introns / Q000235:genetics; D007797:Laminin / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D011544:Pseudogenes / Q000235:genetics*
D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D005819:Genetic Markers; D007797:Laminin; D008562:Membrane Glycoproteins; C058783:laminin beta2; D049030:Dystroglycans; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase