The effect of cyclic AMP on expression of the major immediate-early genes and replication of human cytomegalovirus in human central nervous system cell lines.
The effect of dibutyryl cyclic AMP (dbcAMP) on interaction of human cytomegalovirus (HCMV) with human central nervous system cell lines was examined. Activation of the major immediate-early (IE) promoter (MIEP) of HCMV by dbcAMP was observed in human neuroblastoma IMR-32 cells, but not in glioma 118MGC and astrocytoma U373-MG cells. The 19 bp repeat element in the enhancer of the MIEP was most likely to be the cis-element that responded to dbcAMP in IMR-32 cells as we expected. In IMR-32 cells activation of the MIEP led to enhanced synthesis of the major IE proteins and infectious HCMV.
Department of Microbiology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ishikawa, Japan.
H
H
Sadanari
R
R
Yamada
J
J
Tanaka
T
T
Murayama
K
K
Ohnishi
K
K
Matsubara
S
S
Fukuda
D001254:Astrocytoma; D001932:Brain Neoplasms; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics*; D017781:Genes, Immediate-Early / Q000187:drug effects; D005814:Genes, Viral / Q000187:drug effects; D005910:Glioma; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D007700:Kinetics; D008168:Lung; D009447:Neuroblastoma; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
D017874:Immediate-Early Proteins; D003994:Bucladesine; D000242:Cyclic AMP