Dolichyl sulphate and H-phosphonate: enzymatic reactions with activated sugars.
Two phosphate-modified analogues of dolichyl phosphate were evaluated as substrates or inhibitors of the reactions catalyzed by mammalian microsomal enzymes. Dolichyl H-phosphonate could serve as an efficient acceptor for mannosyl and glucosyl transfer. The reaction products were chromatographically different from those formed from dolichyl phosphate. Lower activity of the H-phosphonate was observed for the reaction of N-acetylglucosaminyl phosphate transfer from UDP-GlcNAc. Dolichyl sulphate was shown not to serve as a substrate for the transfer of mannosyl (from GDP-Man), glucosyl (from UDP-Glc) or N-acetylglucosaminyl phosphate (from UDP-GlcNAc) residues in the presence of rat liver microsomes. Weak inhibitory properties of this analogue were demonstrated.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow. siz@ioc.ac.ru
O V
OV
Sizova
S D
SD
Maltsev
V N
VN
Shibaev
W J
WJ
Jankowski
T
T
Chojnacki
D000818:Animals; D001921:Brain / Q000201:enzymology; D002855:Chromatography, Thin Layer; D004288:Dolichol Phosphates / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D004288:Dolichol Phosphates; D013431:Sulfates; C028306:dolichol monophosphate; D016695:Glycosyltransferases