Microfluidic devices for DNA analysis.
Microfabricated electrophoresis devices allow us to perform short-tandem-repeat genotyping assays in under 2 min and sequence single-stranded DNA in under 15 min. This is 10-100 times faster than standard slab-gel and capillary systems. The microdevice format is the natural extension of 100 years of gradual improvements to electrophoresis but operates in an almost-perfect way, limited only by the sieving medium.
Ehrlich
D J
DJ
Whitehead Institute for Biomedical Research and the Massachussetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA. ehrlich@wi.mit.edu
Matsudaira
P
P
D000180:Acrylic Resins; D001331:Automation; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D020080:Tandem Repeat Sequences; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004247:DNA; D000099166:Polyacrylamides