[A trial of rapid assay of identification and susceptibility test of bacteria detected from blood culture by using VITEK AMS].
For rapid identification and susceptibility test of bacteria detected from bottles of blood culture, we tried a direct method that adjusted bacterial fluid by one time centrifugalization. Identification and susceptibility test were done using VITEK AMS. A result of having compared direct method with a standard method inspected from a colony of medium, an agreement rate was 82.1% of gram negative bacilli, 64.0% of gram positive cocci, 100% of yeast in identification test. In the same way, an agreement rate was 98.0% of gram negative bacilli, 97.2% of gram positive cocci in susceptibility test. The appearance rate of very major error was 0. 7% in gram negative bacilli, and 2.8% in gram positive cocci. As for this method, operation is simple, but it is necessary for confirmed examination in a kind of bacteria. But agreement rate with a standard method is high, direct method is useful to select an appropriate antibiotic until reporting of the last test result of bacteria.
Satoh
K
K
Division of Clinical Microbiology, Department of Medical Technology, Kinki University School of Medicine, Ohno-Higashi 377-2, Osakasayama, Osaka 589-8511, Japan.
Kubo
S
S
Matsushita
K
K
Akiyama
T
T
Furuta
I
I
D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D005658:Fungi / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006095:Gram-Positive Cocci / Q000302:isolation & purification; D006801:Humans; D013997:Time Factors
D003470:Culture Media