Mass determination of rous sarcoma virus virions by scanning transmission electron microscopy.
The internal structural protein of retroviruses, Gag, comprises most of the mass of the virion, and Gag itself can give rise to virus-like particles when expressed in appropriate cells. Previously the stoichiometry of Gag in virions was inferred from indirect measurements carried out 2 decades ago. We now have directly determined the masses of individual particles of the prototypic avian retrovirus, Rous sarcoma virus (RSV), by using scanning transmission electron microscopy. In this technique, the number of scattered electrons in the dark-field image integrated over an individual freeze-dried virus particle on a grid is directly proportional to its mass. The RSV virions had a mean mass of 2.5 x 10(8) Da, corresponding to about 1,500 Gag molecules per virion. The population of virions was not homogeneous, with about one-third to two-thirds of the virions deviating from the mean by more than 10% of the mass in two respective preparations. The mean masses for virions carrying genomes of 7.4 or 9.3 kb were indistinguishable, suggesting that mass variability is not due to differences in RNA incorporation.
Vogt
V M
VM
Section of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA. vmv1@cornell.edu
Simon
M N
MN
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D014771:Virion / Q000648:ultrastructure