Infectivity of Plasmodium berghei sporozoites delivered by intravenous inoculation versus mosquito bite: implications for sporozoite vaccine trials.
Plasmodium berghei sporozoites delivered by mosquito bite were more infectious to outbred CD-1 mice than were sporozoites delivered by intravenous inoculation. The route of challenge also affected vaccine efficacy. In view of these findings and the fact that mosquito bites are the natural mode of sporozoite delivery, infectious mosquito bites should be considered the challenge protocol of choice for sporozoite vaccine efficacy trials.
Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. jvaug001@umaryland.edu
J A
JA
Vaughan
L F
LF
Scheller
R A
RA
Wirtz
A F
AF
Azad
D000818:Animals; D009033:Culicidae / Q000469:parasitology*; D005260:Female; D007114:Immunization; D007299:Insect Bites and Stings; D007303:Insect Vectors; D008288:Malaria / Q000209:etiology / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology* / Q000472:pathogenicity
D017780:Malaria Vaccines