Synergism between mild hyperthermia and interferon-beta gene expression.
In the present study, the synergistic effect of mild hyperthermia in combination with gene expression of interferon-beta (IFN-beta) was examined in vitro in the human glioma cell line U87MG. The cells transiently expressed the IFN-beta gene under the control of the mouse mammary tumor virus promoter and were then subjected to temperature elevation (41 degrees C for 1 h). In terms of the cell killing effect, the optimum scheme was obtained by transfection for 4 days before hyperthermia, i.e. rate in the time course of IFN-beta gene expression. The relative specific growth rate decreased to 32% compared with the control while it was only 40% under the hyperthermic conditions. These observations suggest that IFNbeta expression was able to enhance the sensitivity of transfected glioma cells to mild hyperthermia.
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Japan.
I A
IA
Bouhon
M
M
Shinkai
H
H
Honda
M
M
Mizuno
T
T
Wakabayashi
J
J
Yoshida
T
T
Kobayashi
D000818:Animals; D016923:Cell Death; D002455:Cell Division; D003131:Combined Modality Therapy; D015870:Gene Expression; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D006801:Humans; D006979:Hyperthermia, Induced; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D016899:Interferon-beta