A different mode of entry by dengue-2 neutralisation escape mutant virus.
Entry processes were compared between dengue-2 (DEN-2) virus and a DEN-2 neutralisation escape mutant virus in baby hamster kidney (BHK) cells. The mutant virus (mu6B2) was resistant to neutralisation by a monoclonal antibody, MAb 6B2. Infection of BHK cells by the wild-type DEN-2 virus resulted in direct penetration of the virions into the cytoplasm whereas the mutant virus entered cells by endocytosis. The continual presence of the monoclonal antibody might have asserted some pressure for the mutant to adopt a different mode of entry.
Department of Microbiology, National University of Singapore, Singapore.
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D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure / Q000821:virology; D006224:Cricetinae; D020285:Cryoelectron Microscopy; D003593:Cytoplasm / Q000821:virology; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D004705:Endocytosis; D007668:Kidney; D009500:Neutralization Tests; D014771:Virion / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal