Involvement of caveolae in the uptake of respiratory syncytial virus antigen by dendritic cells.
The uptake of respiratory syncytial virus (RSV) antigen by cattle dendritic cells was investigated. Pathways of antigen uptake were monitored by flow cytometry using specific tracers and by proliferation assays, which were used to measure the presentation of RSV antigen and ovalbumin. Inhibitors that differentially affected pathways were used to distinguish them. Presentation of RSV antigen, but not ovalbumin, was inhibited by phorbol myristate acetate and filipin, which have been reported to inhibit caveolae, but not by cytochalasin D, amiloride, or mannose. These inhibitors have been reported to block macropinocytosis and other actin-dependent uptake mechanisms, endocytic pathways involving clathrin-coated pits, and the mannose receptor. Furthermore, co-localization of RSV antigen and caveolae was observed by confocal microscopy. Thus, the major route for uptake of RSV antigen by cattle dendritic cells is one mediated by caveolae, adding a pathway of antigen uptake by dendritic cells to those established.
Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom.
D
D
Werling
J C
JC
Hope
P
P
Chaplin
R A
RA
Collins
G
G
Taylor
C J
CJ
Howard
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000378:metabolism*; D020126:Brefeldin A / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D004705:Endocytosis; D005372:Filipin / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type; D008351:Mannans / Q000494:pharmacology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D018613:Microscopy, Confocal; D010047:Ovalbumin / Q000378:metabolism; D010873:Pinocytosis; D011956:Receptors, Cell Surface / Q000378:metabolism; D012136:Respiratory Syncytial Viruses / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000956:Antigens, Viral; D002966:Clathrin; D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D020126:Brefeldin A; D015638:Cytochalasin D; D000584:Amiloride; D005372:Filipin; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate; D013755:Tetradecanoylphorbol Acetate