Condensation nucleation light scattering detection for biogenic amines separated by ion-exchange chromatography.
A sensitive method of analysis for biogenic amines, putrescine, cadaverine, histamine and an amino acid precursor, histidine is described herein using ion-exchange chromatography and condensation nucleation light scattering detection. The method was successfully used for the analysis of biogenic amines in fish samples. The method offers a number of advantages: fast elution of analytes with no need for mobile phase conductivity suppression, no derivatization and no electrochemical activity for the analyte's detection. The 3 sigma detection limits for these compounds were found to range from 8 to 20 ng/ml.
Department of Chemistry and Biochemistry, Southern Illinois University at Carbondale 62901-4409, USA.
S K
SK
Sadain
J A
JA
Koropchak
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002103:Cadaverine / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005399:Fishes; D005504:Food Analysis / Q000379:methods*; D006632:Histamine / Q000032:analysis; D006639:Histidine / Q000032:analysis; D008027:Light; D011700:Putrescine / Q000032:analysis; D012542:Scattering, Radiation
D001679:Biogenic Amines; D006639:Histidine; D006632:Histamine; D002103:Cadaverine; D011700:Putrescine