An immunohistochemical study of abnormal keratinocyte differentiation in molluscum contagiosum.
To investigate abnormalities in the keratinization process in lesional epidermis of molluscum contagiosum, production of filaggrin, loricrin, Ted-H-1 antigen, involucrin, cystatin A and CD95 ligand (CD95L) was investigated using specific antibodies. Anti-filaggrin monoclonal antibody (MoAb) did not react with keratohyalin granules (KHG), but with the substance around virus particles in the stratum corneum. KHG reacted with anti-loricrin polyclonal antibody (PoAb) and anti-Ted-H-1 MoAb. Anti-involucrin PoAb and anti-cystatin A PoAb reacted with materials in the cytoplasm of the middle stratum spinosum to the stratum granulosum. CD95L was expressed in the cell membrane region of the living cell layers in lesional epidermis. These observations suggest that the keratinization process may be altered in molluscum contagiosum.
Department of Dermatology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
M
M
Takahashi
A
A
Izutani
T
T
Tezuka
D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002462:Cell Membrane / Q000737:chemistry; D015891:Cystatins / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry; D053222:Fas Ligand Protein; D000091344:Filaggrin Proteins; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D015603:Keratinocytes / Q000737:chemistry / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008976:Molluscum Contagiosum / Q000378:metabolism / Q000473:pathology*; D011498:Protein Precursors / Q000032:analysis
D015415:Biomarkers; D015891:Cystatins; C505661:FASLG protein, human; C000717249:FLG protein, human; D053222:Fas Ligand Protein; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011498:Protein Precursors; C064554:loricrin; C032253:involucrin