Spectrophotometric determination of trace amounts of hemoglobin using the oxidative decomposition reaction of a copper(II)-phthalocyanine complex.
A catalytic effect of hemoglobin was found in the oxidative decomposition of a copper(II)-phthalocyanine complex (Cu-pts) with peroxomonosulfate. Based on this finding, a simple, rapid, highly sensitive and high-precision spectrophotometric method for hemoglobin was developed. In this indicator system, Cu-pts as the indicator and hemoglobin as the catalyst were simultaneously decomposed with peroxomonosulfate. Therefore, the slopes of the absorbance-time curve of the sample solution become equal to that of the blank solution after 5 min. The calibration curve was linear over the range of 12-258 microg/l. Detection limits (3sigma) were 8.13 microg/l, and the relative standard deviation was 2.05% (10 determinations) for 130 microg/l of hemoglobin. The metal ions did not significantly produce an interference in this method. The albumin had some influence but was permissible up to 6 times the weight of hemoglobin. The proposed method was applied to human blood samples with satisfactory results.
Takahata
K
K
Department of Materials Science, Faculty of Engineering, Ibaraki University, Nakanarusawa, Hitachi, Japan.
Igarashi
S
S
D002138:Calibration; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism; D054833:Isoindoles; D016877:Oxidants / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013464:Sulfuric Acids / Q000737:chemistry; D013696:Temperature
D006454:Hemoglobins; D007211:Indoles; D054833:Isoindoles; D016877:Oxidants; D013464:Sulfuric Acids; D003300:Copper; C013647:phthalocyanine