Comparative mass spectrometric analyses of Photofrin oligomers by fast atom bombardment mass spectrometry, UV and IR matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry and laser desorption/jet-cooling photoionization mass spectrometry.
Photofrin (porfimer sodium) is a porphyrin derivative used in the treatment of a variety of cancers by photodynamic therapy. This oligomer complex and a variety of porphyrin monomers, dimers and trimers were analyzed with five different mass spectral ionization techniques: fast atom bombardment, UV and IR matrix-assisted laser desorption/ionization, electrospray ionization, and laser desorption/jet-cooling photoionization. All five approaches resulted in very similar oligomer distributions with an average oligomer length of 2.7 +/- 0.1 porphyrin units. In addition to the Photofrin analysis, this study provides a side-by-side comparison of the spectra for the five different mass spectrometric techniques.
Wyeth-Ayerst Research, Lederle Laboratories, Pearl River, New York 10965, USA. siegelm@war.wyeth.com
M M
MM
Siegel
K
K
Tabei
R
R
Tsao
M J
MJ
Pastel
R K
RK
Pandey
S
S
Berkenkamp
F
F
Hillenkamp
M S
MS
de Vries
D017323:Dihematoporphyrin Ether / Q000032:analysis* / Q000737:chemistry; D011108:Polymers; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D011108:Polymers; D017323:Dihematoporphyrin Ether