Species-specific primers of chitin synthase 1 gene for the differentiation of the Trichophyton mentagrophytes complex.
Species-specific primers were designed from nucleotide sequences of the chitin synthase 1 gene (CHS1) of Arthroderma benhamiae, A. simii and A. vanbreuseghemii. The A. benhamiae-specific primer amplified a 560-bp fragment from A. benhamiae but not from A. simii and A. vanbreuseghemii. The A. simii-specific primers amplified a 470-bp fragment from A. simii but not from A. benhamiae and A. vanbreuseghemii. The A. vanbreuseghemii-specific primers amplified a 360-bp fragment from A. vanbreuseghemii but not from A. benhamiae and A. simii. With these species-specific primers of CHS1 genes, 10 clinical isolates from humans and animals were examined by polymerase chain reaction analyses. These isolates proved to be identical to A. vanbreuseghemii.
Kano
R
R
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Nakamura
Y
Y
Watari
T
T
Watanabe
S
S
Takahashi
H
H
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D003883:Arthrodermataceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D002687:Chitin Synthase / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D003881:Dermatomycoses / Q000382:microbiology; D005800:Genes, Fungal; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014249:Trichophyton / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal; D002687:Chitin Synthase