Complete mineralization of methylparathion by Pseudomonas sp. A3.
Organophosphorus insecticides are widely used in agriculture. Despite their biodegradable nature, some are highly toxic and their residues are found in the environment. Reports on the mineralization of a spectrum of these insecticides by a single potential strain are scarce. We have isolated a soil isolate, Pseudomonas sp. A3, through enrichment technique, able to degrade methylparathion (MP), malathion, monocrotophos, and Diazinon. The potential of this strain to mineralize MP as a carbon and/or phosphorus source has been evaluated. On hydrolysis of MP, the aromatic portion (p-nitrophenol) was used as a carbon and energy source whereas the alkyl moiety (dithiomethylphosphorothioate) was broken down for the phosphorus source. The results from the experiments involving [U-14C]p-nitrophenol provided the evidence for incorporation of carbon into the cellular constituents and release of CO2 from this insecticide. During the breakdown of MP, nitrite was released as a catabolic by-product.
Ramanathan
M P
MP
Department of Pathology, University of Pennsylvania, Philadelphia 19106, USA. ramanath@mail.med.upenn.edu
Lalithakumari
D
D
D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008743:Methyl Parathion / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D009573:Nitrites / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D013055:Spectrophotometry, Infrared
D007306:Insecticides; D009573:Nitrites; D002245:Carbon Dioxide; D010758:Phosphorus; D008743:Methyl Parathion; D002244:Carbon