Determination of bentazon residues in water by high-performance liquid chromatography. Validation of the method.
A method for determination of bentazon residues in water has been developed. The method involves solid-phase extraction with C18 extraction tubes and high-performance liquid chromatographic analysis. A C18 column and guard column were used with UV detection at 230 nm, a mobile phase of methanol-water (60:40, v/v) at pH 4.6 (phosphoric acid) and a flow-rate of 0.8 ml/min. After optimization of the extraction and separation conditions, the method was validated. The method developed can be used for determination of bentazon in water, within the international limits of 0.1 microgram/l, with a 500-fold pre-concentration.
Institute of Chemistry, UNICAMP, Campinas S.P., Brazil.
G M
GM
Pinto
I C
IC
Jardim
D001581:Benzothiadiazines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000032:analysis*
D001581:Benzothiadiazines; D010573:Pesticide Residues; D014874:Water Pollutants, Chemical; C002003:bentazone