Scopoletin: an inducible nitric oxide synthesis inhibitory active constituent from Artemisia feddei.
Bioassay-guided fractionation of an H2O extract of Artemisia feddei has furnished an inducible nitric oxide synthase (iNOS) inhibitory coumarin, scopoletin (1) and one of the inactive sesquiterpenes, achillin (2). Compound 1 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was due to suppression of iNOS mRNA and iNOS protein, as determined by Northern and Western blotting, respectively.
Kang
T H
TH
College of Pharmacy, Wonkwang University, Iksan, Korea.
Pae
H O
HO
Jeong
S J
SJ
Yoo
J C
JC
Choi
B M
BM
Jun
C D
CD
Chung
H T
HT
Miyamoto
T
T
Higuchi
R
R
Kim
Y C
YC
D000818:Animals; D019306:Artemisia; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal; D011994:Recombinant Proteins; D012603:Scopoletin / Q000302:isolation & purification / Q000494:pharmacology*
D008070:Lipopolysaccharides; D010936:Plant Extracts; D011994:Recombinant Proteins; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D012603:Scopoletin