A CD18 monoclonal antibody reduces multiple organ injury in a model of ruptured abdominal aortic aneurysm.
The role of CD18 antibody (anti-CD18) in remote and local injury in a model of ruptured abdominal aortic aneurysm repair was investigated. Rats were divided into sham, shock, clamp, and shock + clamp groups. Shock + clamp animals received anti-CD18 or a control monoclonal antibody. One hour of hemorrhagic shock was followed by 45 min of supramesenteric aortic clamping. Intestinal and pulmonary permeability to (125)I-labeled albumin was determined. Myeloperoxidase (MPO) activity, F(2)-isoprostane levels, and transaminases were also measured. Only shock + clamp resulted in statistically significant increases in pulmonary and intestinal permeability, which were associated with significant increases in MPO activity and F(2)-isoprostane levels. Treatment with anti-CD18 significantly decreased intestinal and pulmonary permeability in shock + clamp animals. These reductions were associated with significantly reduced intestinal and hepatic MPO activity and pulmonary F(2)-isoprostane levels and reduced alanine and aspartate aminotransferase levels; however, anti-CD18 had no effect on intestinal or hepatic F(2)-isoprostane levels or on pulmonary MPO activity. These results suggest CD18-dependent and -independent mechanisms of local and remote organ injury in this model of ruptured abdominal aortic aneurysm.
Boyd
A J
AJ
Division of Vascular Surgery, Department of Surgery, The Toronto Hospital (General Division), Faculty of Medicine, University of Toronto, Toronto, Ontario M5C 2C4, Canada.
Rubin
B B
BB
Walker
P M
PM
Romaschin
A
A
Issekutz
T B
TB
Lindsay
T F
TF
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D017544:Aortic Aneurysm, Abdominal / Q000150:complications*; D001019:Aortic Rupture / Q000150:complications*; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure; D016827:CD8 Antigens / Q000276:immunology*; D007422:Intestines / Q000473:pathology; D008099:Liver / Q000473:pathology; D008168:Lung / Q000201:enzymology; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology* / Q000517:prevention & control*; D010539:Permeability; D009195:Peroxidase / Q000502:physiology; D011453:Prostaglandins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D011453:Prostaglandins; D009195:Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase