Characterization of bovine coronavirus isolates/from eight different states in the USA.
Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few hygromycin B resistant isolates were also found.
Department of Diagnostic Medicine-Pathobiology College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA. kapil@vet.ksu.edu
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S
Kapil
K L
KL
Richardson
T R
TR
Maag
S M
SM
Goyal
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D018352:Coronavirus Infections / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000737:chemistry / Q000145:classification* / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D006801:Humans; D006921:Hygromycin B / Q000494:pharmacology; D007525:Isoelectric Focusing / Q000662:veterinary; D051379:Mice; D014407:Tumor Cells, Cultured; D014481:United States; D014764:Viral Proteins / Q000032:analysis
D000914:Antibodies, Viral; D014764:Viral Proteins; D006921:Hygromycin B