Oxidation of phenols by horseradish peroxidase and lactoperoxidase compound II--kinetic considerations.
Oxidation of para substituted phenols by horseradish peroxidase compound II (HRP-II) and lactoperoxidase compound II (LPO-II) were studied using stopped flow technique. Apparent second order rate constants (kapp) of the reactions were determined. The kinetics of oxidation of phenols by HRP-II and LPO-II have been compared with the oxidation potentials of the substrates. Reorganization energies of electron-transfer of phenols to the enzymes were estimated from the variation of second order rate constants with the thermodynamic driving force.
Department of Chemical Sciences, Tata Institute of Fundamental Research, Navy Nagar, Bombay.
M S
MS
Zahida
W
W
Deva
G M
GM
Peerzada
D V
DV
Behere
D006735:Horseradish Peroxidase / Q000378:metabolism*; D007700:Kinetics; D007784:Lactoperoxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*
D010636:Phenols; D006735:Horseradish Peroxidase; D007784:Lactoperoxidase