Measurement of blood hematocrit inside the magnetically suspended centrifugal pump using an optical technique: application to assessment of pump flow.
To measure blood hematocrit inside the magnetically suspended centrifugal pump, we have performed both forward and backward light scattering measurements using a specially designed optical cell. In the forward scattering measurement, an optical fiber was used to guide the near infrared light at 780 nm into a 250 microns gap region, and the light that forward scattered toward a detector fiber was measured using a phototransistor. The light intensity decreased exponentially with an increase in the hematocrit to around 20%. The forward scattering method suffered from sensitivity at the hematocrit levels around 25-45% due to the diffusion effect. By making the optical path length larger than several millimeters, the sensitivity of the forward scattering method in terms of hematocrit change can be improved. In the back scattering method, however, better sensitivity in terms of hematocrit change from 0-50% was obtained. By making the optical fiber separation distance less than 1 mm, the system will measure the first order back scattering from the shallow layer while, by making the fiber separation distance larger than several millimeters, the system will primarily measure the diffuse reflectance from the deeper layer. Both approaches will yield sensitive optical intensity change in terms of the physiological hematocrit range.
Kaiwa
T
T
Yamagata University, Biomedical Systems Engineering, Japan.
Mori
T
T
Kijima
T
T
Nogawa
M
M
Nojiri
C
C
Takatani
S
S
D000465:Algorithms; D000818:Animals; D001243:Assisted Circulation / Q000295:instrumentation*; D002417:Cattle; D004058:Diffusion; D005336:Fiber Optic Technology / Q000295:instrumentation; D006400:Hematocrit; D007259:Infrared Rays; D008280:Magnetics / Q000295:instrumentation*; D008954:Models, Biological; D055100:Optical Fibers; D055095:Optics and Photonics / Q000295:instrumentation; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic