Detection of DNA in agarose gels using berberine and Mordant Yellow 3R.
A nontoxic and simple staining method for the detection of DNA in agarose gels is described. After eletrophoretic separation, the gels were stained with 5 microg/ml of berberine (BB) prepared in distilled water and then the gels were soaked in 20 microg/ml of aqueous Mordant Yellow 3R (MY3R) solution. Employment of MY3R as a counterion dye efficiently quenched unwanted background fluorescence of BB. This method can detect as little as 10 ng of plasma membrane H(+)-ATPase cDNA obtained from Arabidopsis thaliana L. (AHA1, 3.2 kb) under a long wavelength of UV irradiation (366 nm) within 1 h.
Jung
D W
DW
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, 500-757, Korea.
Yoo
G S
GS
Choi
J K
JK
D017360:Arabidopsis / Q000201:enzymology; D001391:Azo Compounds; D001599:Berberine; D004396:Coloring Agents; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000032:analysis* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D007202:Indicators and Reagents; D006180:Proton-Translocating ATPases / Q000235:genetics; D012680:Sensitivity and Specificity
D001391:Azo Compounds; D004396:Coloring Agents; D018076:DNA, Complementary; D007202:Indicators and Reagents; D001599:Berberine; C072726:mordant yellow 3; D004247:DNA; D006180:Proton-Translocating ATPases