Detection and characterization of a mannan-binding lectin from the mosquito, Anopheles stephensi (Liston).
Two lectins from the serum of the mosquito, Anopheles stephensi (Liston), with distinct characteristics, were detected by agglutination of various animal erythrocytes. The lectins were developmental stage-specific and/or sex-related. One adult female-specific lectin was identified as mannan-specific, and named mosquito mannan-binding lectin (MBL). MBL cross-reacted immunologically with antibodies against a previously characterized cockroach lectin, Blaberus discoidalis lectin (BDL1), and its activity was almost completely blocked by the antibodies. Mosquito MBL agglutinated erythrocytes from human, sheep, goat and rabbit, but not chicken or mouse, and agglutination was inhibited by mannan and nitrophenol-modified sugar derivatives, but not by simple sugars. Using affinity chromatography with immobilized mannan on Sepharose 6B, the mosquito MBL was partially purified. Purified mosquito MBL shared biochemical properties with BDL1, containing two subunits of molecular mass of 28 and 30 kDa under reducing conditions in SDS/PAGE. Its activity is dependent on Ca(2+), and it is stable at pH 7-9 and at temperatures less than 30 degrees C.
Chen
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Department of Zoology, University of Aberdeen, UK.
Billingsley
P F
PF
D000367:Age Factors; D000818:Animals; D000852:Anopheles / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D037222:Collectins; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006386:Hemagglutination Tests; D006458:Hemolymph / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D013696:Temperature; D013997:Time Factors
D002352:Carrier Proteins; D037222:Collectins