Refolding of recombinant alpha and beta subunits of the Rhodospirillum rubrum F(0)F(1) ATP synthase into functional monomers that reconstitute an active alpha(1)beta(1)-dimer.
The alpha subunit from the Rhodospirillum rubrum F(0)F(1) ATP synthase (RrF(1)alpha) was over-expressed in unc operon-deleted Escherichia coli strains under various growth conditions only in insoluble inclusion bodies. The functional refolding of urea-solubilized RrF(1)alpha was followed by measuring its ability to stimulate the restoration of ATP synthesis and hydrolysis in beta-less R. rubrum chromatophores reconstituted with pure native or recombinant RrF(1)beta [Nathanson, L. & Gromet-Elhanan, Z. (1998) J. Biol. Chem. 273, 10933-10938]. The refolding efficiency was found to increase with decreasing RrF(1)alpha concentrations and required high concentrations of MgATP, saturating approximately 60% when 50 microgram protein.mL(-1) were refolded in presence of 50 mM MgATP. Size-exclusion HPLC of such refolded RrF(1)alpha revealed a 50-60% decrease in its aggregated form and a parallel appearance of its monomeric peak. RrF(1)beta refolded under identical conditions appeared almost exclusively as a monomer. This procedure enabled the isolation of large amounts of a stable RrF(1)alpha monomer, which stimulated the restoration of ATP synthesis and hydrolysis much more efficiently than the refolded alpha mixture, and bound ATP and ADP in a Mg-dependent manner. Incubation of both RrF(1)alpha and beta monomers, which by themselves had no ATPase activity, resulted in a parallel appearance of activity and assembled alpha(1)beta(1)-dimers, but showed no formation of alpha(3)beta(3)-hexamers. The RrF(1)-alpha(1)beta(1)-ATPase activity was, however, very similar to the activity observed in isolated native chloroplast CF(1)-alpha(3)beta(3), indicating that these dimers contain only the catalytic nucleotide-binding site at their alpha/beta interface. Their inability to associate into an alpha(3)beta(3)-hexamer seems therefore to reflect a much lower stability of the noncatalytic RrF(1) alpha/beta interface.
Du
Z
Z
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel.
Gromet-Elhanan
Z
Z
D000255:Adenosine Triphosphate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008956:Models, Chemical; D017510:Protein Folding; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D012247:Rhodospirillum rubrum / Q000201:enzymology*; D013997:Time Factors
D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases