Structure of the native (unligated) mannose-specific bulb lectin from Scilla campanulata (bluebell) at 1.7 A resolution.
The X-ray crystal structure of native Scilla campanulata agglutinin, a mannose-specific lectin from bluebell bulbs and a member of the Liliaceae family, has been determined by molecular replacement and refined to an R value of 0.186 at 1.7 A resolution. The lectin crystallizes in space group P21212 with unit-cell parameters a = 70. 42, b = 92.95, c = 46.64 A. The unit cell contains eight protein molecules of Mr = 13143 Da (119 amino-acid residues). The asymmetric unit comprises two chemically identical molecules, A and B, related by a non-crystallographic twofold axis perpendicular to c. This dimer further associates by crystallographic twofold symmetry to form a tetramer. The fold of the polypeptide backbone closely resembles that found in the lectins from Galanthus nivalis (snowdrop) and Hippeastrum (amaryllis) and contains a threefold symmetric beta-prism made up of three antiparallel four-stranded beta-sheets. Each of the four-stranded beta-sheets (I, II and III) possesses a potential saccharide-binding site containing conserved residues; however, site II has two mutations relative to sites I and III which may prevent ligation at this site. Our study provides the first accurate and detailed description of a native (unligated) structure from this superfamily of mannose-specific bulb lectins and will allow comparisons with a number of lectin-saccharide complexes which have already been determined or are currently under investigation.
Wood
S D
SD
School of Biomolecular Sciences, Max Perutz Building, Liverpool John Moores University, Liverpool L3 3AF, England.
Wright
L M
LM
Reynolds
C D
CD
Rizkallah
P J
PJ
Allen
A K
AK
Peumans
W J
WJ
Van Damme
E J
EJ
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D027801:Galanthus; D016018:Least-Squares Analysis; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D037121:Plant Lectins; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D037102:Lectins; D037121:Plant Lectins; D008358:Mannose