A novel stimulus, oncogene, induces expression of CD80 (B7-1) gene.
CD80, a cell surface molecule found on antigen-presenting cells which particpates in costimulatory signaling, is frequently detected on transformed and tumor cells. We examined the effect of transformation by v-myc, k-ras, and SV40 T antigen oncogenes on the expression of a CD80 promoter/luciferase gene as well as the endogenous CD80 promoter gene in murine fibroblast cell lines. All three transformed cell lines were tumorigenic in nude mice, however expression of the CD80/luciferase transgene or endogenous CD80 was detected only in the v-myc and k-ras transformed cell lines. In both cell lines, CD80 expression was first detected more than 30 passages after transformation. Therefore, induction of CD80 expression was a late event following transformation and was not essential for the establishment of the transformed phenotype.
Department of Growth Factor Research, Pharmaceutical Products Division, Abbott Laboratories D-4MG, AP9A, Abbott Park, Ill. 60064, USA. HaiyingZhang@abbott.com
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Zhang
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Haasch
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Patterson
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Dickinson
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Kifle
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Fry
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Okasinski
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009857:Oncogenes; D011401:Promoter Regions, Genetic
D018122:B7-1 Antigen; D008156:Luciferases