Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor-derived gametes.
Blastomere transplantation into fish blastula embryos results in somatic chimeras, which generally provide null or a small proportion of gametes derived from the donor. This may partly explain why none of the ES-like cell lines established from fish embryos has contributed to the germline of chimeras when transplanted at the blastula stage. Here, we report that a moderate gamma-irradiation of recipient embryos, followed by transplantation of dispersed blastomeres, considerably enhances the proportion of donor-derived gametes (53% versus 5% in average). In fish, the resulting protocol should maximise the pluripotency level measured in vivo for embryonic cell lines and for cultured germ cells.
Laboratoire de Génétique des Poissons, Domaine de Vilvert, I.N.R.A., Jouy-en-Josas Cedex, France. joly@jouy.inra.fr
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JS
Joly
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Kress
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Vandeputte
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Bourrat
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Chourrout
D000818:Animals; D001757:Blastomeres / Q000637:transplantation*; D017690:Cell Transplantation / Q000379:methods; D005260:Female; D005720:Gamma Rays; D006066:Gonads / Q000196:embryology* / Q000528:radiation effects; D008297:Male; D009990:Oryzias / Q000196:embryology* / Q000235:genetics; D010858:Pigmentation; D011828:Radiation Chimera; D033581:Stem Cell Transplantation