Mutation of the mitochrondrially encoded ATPase 6 gene modeled in the ATP synthase of Escherichia coli.
Defects of respiratory chain protein complexes and the ATP synthase are becoming increasingly implicated in human disease. Recently, mutations in the ATPase 6 gene have been shown to cause several different neurological disorders. The product of this gene is homologous to the a subunit of the ATP synthase of Escherichia coli. Here, mutations equivalent to those described in humans have been introduced into the a subunit of E. coli by site-directed mutagenesis, and the effects of these mutations on the ATPase activity, ATP synthesis and ability of the enzyme to pump protons studied in detail. The effects of the mutations varied considerably. The mutation L262P (9185 T-C equivalent) caused a 70% loss of ATP synthesis activity, reduced DCCD sensitivity, and lowered proton pumping activity. The L207P (8993 T-C equivalent) reduced ATP synthesis by 50%, affected DCCD sensitivity, while proton pumping was only marginally affected when measured by the standard AMCA quenching assay. The other mutations studied affected the functioning of the ATP synthase much less. The results confirm that modeling of these point mutations in the E. coli enzyme is a useful approach to determining how alterations in the ATPase 6 gene affect enzyme function and, therefore, how a pathogenic effect can be exerted.
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
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Ogilvie
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Capaldi
D001426:Bacterial Proteins / Q000235:genetics*; D025243:Bacterial Proton-Translocating ATPases; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D006801:Humans; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D009422:Nervous System Diseases / Q000235:genetics*; D017354:Point Mutation; D017494:Proton Pumps / Q000235:genetics; D018892:Proton-Motive Force / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D017494:Proton Pumps; D025243:Bacterial Proton-Translocating ATPases; C074428:atpB protein, E coli