[Clinical, epidemiological and microbiological aspects of Mobiluncus sp. in bacterial vaginosis].
BACKGROUND
In this paper, our goal was to determine the optimal isolation conditions, biochemical characterization, and preservation of species of the genus Mobiluncus, associated with bacterial vaginosis in patients attending the family planning clinic. Also, we tried to relate its presence with demographic variables and criteria used in the clinical diagnosis of bacterial diagnosis.


METHODS
The specimen from the posterior fornix were collected and transported to the laboratory in a Stuart medium, one at room temperature and the other at 4 degrees C. These samples were inoculated in anaerobic culture media.


RESULTS
Of a total of 92 patients studied, 61 (66.3%) were normal, 28 (30.4%) bacterial vaginosis, and 3 (3.3%) had intermediate vaginosis. There was statistically significant relationship only with intrauterine device use (p = 0.00499). The presence of curved rod, using Gram's method, was significantly related with pH (p = 0.00000) positive amines test (p = 0.00000), and the presence of clue cells (p = 0.00000). Mobiluncus was observed in 23 samples (82%), and the majority (15) using RLK agar (cold enrichment technique). With conventional techniques, we identified 12 strains as Mobiluncus curtisii and 3 strains as Mobiluncus mulieris. The strains of Mobiluncus sp. grew better from litmus milk conserved at -30 degrees C.


CONCLUSIONS
Isolating Mobiluncus sp. is fairly easy, if the right media and the techniques are used.
Menolascina
A
A
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Escuela de Bioanálisis, Universidad de Los Andes, Mérida, Venezuela.
Nieves
B
B
Velazco
E
E
Rivero
N
N
Calderas
Z
Z
D001431:Bacteriological Techniques; D016866:Bacteroidaceae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D006801:Humans; D017904:Mobiluncus / Q000302:isolation & purification*; D016585:Vaginosis, Bacterial / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology
Aspectos clínicos, epidemiológicos y microbiológicos de Mobiluncus sp. en la vaginosis bacteriana.