Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture.
Transplantation of osteogenic cells with a suitable matrix is one strategy for engineering bone tissue. Three-dimensional distribution and growth of cells within the porous scaffold are of clinical significance for the repair of large bony defects. A nano-HAp/collagen (nHAC) composite that mimics the natural bone both in composition and microstructure to some extent was employed as a matrix for the tissue engineering of bone. A porous nHAC composite was produced in sheet form and convolved to be a three-dimensional scaffold. Using organ culture techniques and the convolving method, we have developed three-dimensional osteogenic cells/nHAC constructs in vitro. Scanning electron microscopic and histological examination has demonstrated the development of the cells/material complex. Spindle-shaped cells migrating out of bone fragments continuously proliferated and migrated throughout the network of the coil. The porous nHAC scaffold provided a microenvironment resembling that seen in vivo, and cells within the composite eventually acquired a tridimensional polygonal shape. In addition, new bone matrix was synthesized at the interface of bone fragments and the composite.
Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China.
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Du
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Cui
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XD
Zhu
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de Groot
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes; D002455:Cell Division; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques / Q000379:methods*; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology* / Q000637:transplantation; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite