Errors and reproducibility in electron-density map interpretation.
Three investigators, with varying levels of experience, independently built and refined the structure of Escherichia coli ribokinase at 2.6 A resolution. At the end of the refinement/rebuilding processes the models had essentially converged, although each had its own particular pattern of remaining errors. The subsequent refinement of the same structure at 1.8 A resolution allowed an overall quality check of each of the lower resolution models, and an analysis of which graphics-based tools were generally most efficient in locating these errors. Criteria which are useful in the application of Ramachandran, main-chain and side-chain database and real-space fit analyses are presented.
Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Centre, Box 590, S-751 24 Uppsala, Sweden. mowbray@xray.bmc.uu.se
S L
SL
Mowbray
C
C
Helgstrand
J A
JA
Sigrell
A D
AD
Cameron
T A
TA
Jones
D018360:Crystallography, X-Ray; D004583:Electrons; D004926:Escherichia coli / Q000201:enzymology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry*; D011487:Protein Conformation; D015203:Reproducibility of Results
D017853:Phosphotransferases (Alcohol Group Acceptor); C022534:ribokinase