Use of polymerase chain reaction for citrate synthase gene to diagnose Bartonella quintana endocarditis.
We describe aortic valve endocarditis caused by Bartonella quintana in a 31-year-old man. The diagnosis was made on the basis of polymerase chain reaction amplification of the B quintana citrate synthase gene from cardiac valve tissue, the compatibility of histochemical stains of cardiac valve tissue, and serologic studies.
Division of Infectious Diseases, Mayo Clinic and Foundation, Rochester, MN 55902, USA.
R
R
Patel
J O
JO
Newell
G W
GW
Procop
D H
DH
Persing
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D001021:Aortic Valve / Q000382:microbiology* / Q000473:pathology; D018413:Bartonella quintana / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification; D002950:Citrate (si)-Synthase / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000382:microbiology*; D015658:HIV Infections / Q000150:complications; D006349:Heart Valve Diseases / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D014205:Trench Fever / Q000175:diagnosis / Q000382:microbiology*
D000942:Antigens, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D002950:Citrate (si)-Synthase