[Expression and functions of adaptive response genes in Escherichia coli treated with mono- and bifunctional alkylating agents. Interference with SOS response].
The expression of genes belonging to the Ada regulon of Escherichia coli under the action of mono- and bifunctional alkylating agents--high-efficiency antitumor HMM, ACNU, and BCNU preparations--was studied. The functional specificity of the alkA, alkB, and aidB1 genes concerning both the structure and volume of DNA alkylation and the specificity of cell preadaptation was revealed. Additional experimental evidence for the role of the aidB1 gene as a unique "hazard gene", a component of the E. coli ada operon, was obtained. A phenomenon of positive interference between alternative SOS and Ada responses was observed for the first time upon gene expression.
Vasil'eva
S V
SV
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
Makhova
E V
EV
Moshkovskaia
E Iu
EIu
D000222:Adaptation, Physiological; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D005075:Biological Evolution; D002330:Carmustine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015376:Nimustine / Q000494:pharmacology; D009876:Operon; D013014:SOS Response, Genetics / Q000187:drug effects*
D018906:Antineoplastic Agents, Alkylating; D015376:Nimustine; D002330:Carmustine
Ekspressiia i funktsii genov adaptivnogo otveta v Escherichia coli pri deĭstvii mono- i bifunktsional'nykh akliliruiushchikh agentov. Interferentsiia s SOS-otvetom.