Use of polymerase chain reaction for citrate synthase gene to diagnose Bartonella quintana endocarditis.
We describe aortic valve endocarditis caused by Bartonella quintana in a 31-year-old man. The diagnosis was made on the basis of polymerase chain reaction amplification of the B quintana citrate synthase gene from cardiac valve tissue, the compatibility of histochemical stains of cardiac valve tissue, and serologic studies.
Patel
R
R
Division of Infectious Diseases, Mayo Clinic and Foundation, Rochester, MN 55902, USA.
Newell
J O
JO
Procop
G W
GW
Persing
D H
DH
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D001021:Aortic Valve / Q000382:microbiology* / Q000473:pathology; D018413:Bartonella quintana / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification; D002950:Citrate (si)-Synthase / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000382:microbiology*; D015658:HIV Infections / Q000150:complications; D006349:Heart Valve Diseases / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D014205:Trench Fever / Q000175:diagnosis / Q000382:microbiology*
D000942:Antigens, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D002950:Citrate (si)-Synthase