Detection of dendritic cells in the non-obese diabetic (NOD) mouse islet pancreas infiltrate is correlated with Th2-cytokine production.
We investigate the role played by dendritic cells (DCs) in the non-obese diabetic (NOD) mouse pancreas. The early peri-islet, nondestructive infiltration phase, and intra-islet, destructive infiltration phase, which immediately precedes overt diabetes, are studied. Results show that infiltrating cells are Ia-b, ICAM-1, and, mainly, MIDC-8 immunoreactive (ir). These data from silica-treated animals and ultrastructural observations strongly support the hypothesis that DCs are both Ia-b-ir and ICAM-1-ir and that they exert a pivotal role during the period of early infiltration. This is a novel finding for NOD mice and increases the interest for this protective cell type during the rather complex islet infiltration process. Moreover, the cytokine profile demonstrates that Th2 protective cytokines are specific for peri-islet infiltrate. Disappearance of DCs from the infiltrate is concomitant with both the formation of intra-islet infiltration and the increase in proinflammatory Th1 cytokine levels. This further supports the hypothesis that DCs may exert a protective role against diabetes development.
Papaccio
G
G
Institute of Histology and Embryology, School of Medicine, Second University of Naples, 80138 Naples, Italy. gpapacc@tin.it
De Luca
A
A
De Luca
B
B
Pisanti
F A
FA
Zarrilli
S
S
D000367:Age Factors; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells; D005260:Female; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008854:Microscopy, Electron; D010179:Pancreas / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology; D018418:Th2 Cells / Q000378:metabolism*; D013997:Time Factors
D001786:Blood Glucose; D016207:Cytokines; D018799:Intercellular Adhesion Molecule-1; D012822:Silicon Dioxide