Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR.
A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenic E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha, beta, and gamma intimin variants.
Institute of Molecular Evolutionary Genetics, Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
S D
SD
Reid
D J
DJ
Betting
T S
TS
Whittam
D018829:Adhesins, Bacterial; D000483:Alleles; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002352:Carrier Proteins; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D006801:Humans; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli