In situ detection of human monocyte/macrophage serine esterase-1 mRNA expression in human tissues.
Human monocyte/macrophage serine esterase (HMSE) can be distinguished from esterases of other blood cells by a specific isoelectric focusing pattern of five enzyme variants. Using a HMSE-1 cDNA, expression of mRNA was investigated by nonradioactive in situ hybridization of human biopsy specimens and cytospin preparations. HMSE-1 transcripts could be detected in the myelomonocytic cell line U-937, blood monocytes and in tissue macrophages. Immune accessory cells of the monocyte/macrophage lineage did not express detectable amounts of HMSE-1 mRNA. These results indicate that HMSE-1 represents a cell-lineage-specific enzyme which can be used to characterize physiological and neoplastic variants of the human monocyte/macrophage cell system. The method described will enable further insight into the physiological function of HMSE-1.
Department of Pathology, Georg August University of Göttingen, Germany.
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T
Satoh
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B
Hemmerlein
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F
Zschunke
H J
HJ
Radzun
D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000201:enzymology; D004950:Esterases / Q000235:genetics*; D015870:Gene Expression; D006099:Granuloma / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008221:Lymphoid Tissue / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology*; D009000:Monocytes / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000201:enzymology
D012333:RNA, Messenger; D004950:Esterases; C022635:serine esterase