The V3 loop based multi-epitope polypeptide TAB9 adjuvated with montanide ISA720 is highly immunogenic in nonhuman primates and induces neutralizing antibodies against five HIV-1 isolates.
In a previous work we selected montanide ISA720 (M-ISA720) among different adjuvants for the vaccination with a V3 loop based multi-epitope polypeptide TAB9. In this paper we present the evaluation of the toxicity and immunogenicity of this formulation in non-human primates. TAB9 in M-ISA720 was highly immunogenic in macaques (Macaca fascicularis) inducing antibodies against TAB9 in all animals after one inoculation and a strong anamnestic response after booster injections. Furthermore 97% of the V3 peptides included were recognized by TAB9 sera. No differences between doses of 200 microg and 1 mg of TAB9 in M-ISA720 were observed after four immunizations. Neutralizing antibodies against five HIV-1 isolates were detected in most animals. Animals remain healthy throughout the study and did not show lesions at the inoculation site.
Departamento de SIDA, Centro de Ingeniería Geńetica y Biotecnología, Ciudad Habana, Cuba. carlos.duarte@cigb.edu.cu
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D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000915:Antibody Affinity; D016384:Consensus Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology*; D008255:Macaca radiata; D008353:Mannitol / Q000031:analogs & derivatives* / Q000276:immunology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009821:Oils; D009829:Oleic Acids / Q000276:immunology*; D010455:Peptides / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016056:Immunodominant Epitopes; D009821:Oils; D009829:Oleic Acids; D010455:Peptides; C001681:mannide monooleate; D008353:Mannitol