Derivative manipulation in the structure solution of the integral membrane LH2 complex.
The structure of the peripheral light-harvesting complex from Rhodopseudomonas acidophila strain 10050 was determined by multiple isomorphous replacement methods. The derivatization of the crystals was augmented by the addition of a backsoaking stage. The soak/backsoaked data comparison had greater isomorphism and showed simpler Patterson maps than the standard native/soak comparison. Amplitudes from the derivatized then backsoaked crystals and from the derivatized crystals were compared in order to extract a subset of heavy-atom sites. Using this information, the full array of sites were found from a derivative/native comparison, eventually leading to excellent electron-density maps.
Prince
S M
SM
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, Scotland.
McDermott
G
G
Freer
A A
AA
Papiz
M Z
MZ
Lawless
A M
AM
Cogdell
R J
RJ
Isaacs
N W
NW
D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification; D017433:Protein Structure, Secondary; D012241:Rhodopseudomonas / Q000737:chemistry
D045322:Photosynthetic Reaction Center Complex Proteins