Arginine deiminase inhibits cell proliferation by arresting cell cycle and inducing apoptosis.
We have previously demonstrated that arginine deiminase inhibits the proliferation of vascular endothelial cells, but the mechanisms leading to growth inhibition have remained unclear. We report here that low concentrations of arginine deiminase purified from Mycoplasma arginini inhibit proliferation of various cultured cells by arresting the cell cycle in G(1) and/or S phase with higher arginine deiminase concentrations leading to subsequent apoptosis. Our results demonstrate that arginine deiminase inhibits cell proliferation not only by depletion of arginine, but also by mechanisms involving the cell cycle and death signals.
Department of Hematology, Oncology and Endocrinology, Department of Radiation Biology, Department of Microbiology, Children's Hospital, University of Essen, Hufelandstrasse 55, Essen, 45122, Germany.
H
H
Gong
F
F
Zölzer
G
G
von Recklinghausen
J
J
Rössler
S
S
Breit
W
W
Havers
T
T
Fotsis
L
L
Schweigerer
D017209:Apoptosis / Q000187:drug effects*; D001120:Arginine / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006867:Hydrolases / Q000494:pharmacology*; D009174:Mycoplasma / Q000201:enzymology; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured
D001120:Arginine; D006867:Hydrolases; C019723:arginine deiminase