Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C.
OBJECTIVE
To evaluate the functional and histological effects of a single application of topical mitomycin-C after laser injury in the posterior canine glottis.


METHODS
A prospective, randomized study of 16 canines.


METHODS
A supersaturated (1%) solution of topical mitomycin-C was applied to a unilateral, laser-induced injury near the cricoarytenoid joint in eight dogs. The mitomycin-soaked pledget was placed immediately after induction of the injury and was left in contact with exposed cartilage for 3 minutes. The opposite side was not injured to provide an internal control. In eight additional dogs, the same laser injury was allowed to heal untreated. After 6 weeks, the animals were sacrificed and their larynges harvested. Arytenoid adduction sutures were placed bilaterally, and the force required to bring the vocal folds to midline was measured for each side using tensiometry. Gross and microscopic histological analysis was performed. Statistical analysis was accomplished using a two-tailed Student t test of unpaired samples, and the Wilcoxon Signed Rank Test where appropriate.


RESULTS
The mitomycin-C treated larynges demonstrated improved cricoarytenoid joint mobility (P = .007), decreased granulation tissue development (P = .03), and complete prevention of secondary "vocal granuloma" formation (P = .0004) when compared with eight dogs with identical laser injuries allowed to heal untreated. No complications were noted.


CONCLUSIONS
This study demonstrates functional preservation and improved histological appearance of the injured glottis after a single treatment with topical mitomycin-C. Potential applications of these findings include prophylactic use of topical mitomycin-C on glottic insults that commonly progress from granulation tissue formation to scarring and decreased vocal fold function.
Department of Otolaryngology, Vanderbilt Bill Wilkerson Center for Otolaryngology and Communication Sciences, Vanderbilt University Medical Center, Nashville, Tennessee 37232-2559, USA.
J E
JE
Spector
J A
JA
Werkhaven
N C
NC
Spector
S
S
Huang
R N
RN
Page
B
B
Baranowski
M
M
Luther
B
B
McGehee
L
L
Reinisch
D000287:Administration, Topical; D000818:Animals; D001696:Biomechanical Phenomena; D004285:Dogs; D005931:Glottis / Q000187:drug effects* / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D007834:Lasers; D016685:Mitomycin / Q000008:administration & dosage*; D011897:Random Allocation; D014945:Wound Healing / Q000187:drug effects
D016685:Mitomycin