Allelic polymorphisms and RFLP in the human immunoglobulin lambda light chain locus.
The organization of the human immunoglobulin lambda light chain locus (IGL) was recently described. This locus has been entirely sequenced. To evaluate the extent of the genomic variability existing inside that locus, we compiled all the available sequences of germline IGLV genes to find variants of Vlambda sequences. We also looked for RFLP polymorphisms in a reputedly highly polymorphic human population from eastern Senegal, and compiled all RFLP data previously published. Analysis of these data indicates that IGLV alleles are frequent and increase the diversity of the lambda light chain repertoire in the human population. In contrast, RFLP and polymorphism by insertion and/or deletion are limited in that locus. This observation reinforces our hypothesis that the human IGL locus has undergone less evolutionary shuffling than the human kappa or heavy-chain loci.
Laboratoire d'ImmunoGénétique Moléculaire, LIGM, Université Montpellier II, UPR CNRS 1142, IGH, France.
M P
MP
Lefranc
N
N
Pallarès
J P
JP
Frippiat
D000483:Alleles; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005006:Ethnicity / Q000235:genetics; D005803:Genes, Immunoglobulin; D014644:Genetic Variation; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D044469:Racial Groups / Q000235:genetics; D015183:Restriction Mapping; D017384:Sequence Deletion
D004251:DNA Transposable Elements; D007146:Immunoglobulin lambda-Chains; D004262:DNA Restriction Enzymes