Postsurgical adhesion formation in germfree and ex-germfree rats--a study using three scoring scales.
Postsurgical adhesions occur commonly after surgical procedures and are the source of substantial postoperative morbidity. No preventive or prophylactic regimen against adhesions has proven successful in all circumstances. The reasons for this are not clear. The basic mechanisms causing adhesion formation have not been elucidated fully, and furthermore, lack of accurate methods of measuring adhesions may be a contributing factor. Postoperative adhesions may occur in all kinds of surgery but are especially prominent in the abdomen, where the bowel flora may be a compromising factor. This study was undertaken to study the influence of the gastrointestinal microflora on adhesion formation. Germfree and ex-germfree DA rats were subjected to a cecal crush model, and adhesions were evaluated after 7 days using 3 different scoring scales. Germfree rats formed significantly fewer adhesions than their ex-germfree (conventionalized) counterparts. The differences were so great that all three scoring scales achieved significance (p < .005). This study corroborates that the endogenous bowel flora per se is involved in adhesion formation without causing frank infection.
Bothin
C G
CG
Department of Surgery, Karolinska Institutet at Huddinge University Hospital, Sweden. Tore.Midtvedt@cmb.ki.se
Okada
M
M
Midtvedt
T
T
D000818:Animals; D004064:Digestive System / Q000382:microbiology*; D005260:Female; D008297:Male; D011183:Postoperative Complications / Q000382:microbiology*; D051381:Rats; D011919:Rats, Inbred Strains; D000267:Tissue Adhesions / Q000382:microbiology*