Identification of CARE-2-negative Candida albicans isolates as Candida dubliniensis.
Among 302 clinical yeast isolates originally identified as Candida albicans, we found 16 isolates that did not hybridize with the C. albicans-specific repetitive DNA element CARE-2. These 16 isolates produced abundant chlamydospores, did not grow at 43 degrees C, and exhibited a distinct randomly amplified polymorphic DNA profile. Sequence analysis of part of the 28S rDNA demonstrated that the CARE-2-negative isolates are not an atypical subgroup of C. albicans but belong to the recently described new species C. dubliniensis.
Zentrum für Infektionsforschung, Universität Würzburg, Germany.
J
J
Morschhäuser
M
M
Ruhnke
S
S
Michel
J
J
Hacker
D002175:Candida / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification* / Q000235:genetics; D002177:Candidiasis / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S