Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola.
IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4 bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.
Departamento de Biología Funcional (Area de Microbiología) and Instituto Universitario de Biotecnología (IUBA), Universidad de Oviedo, E-33006 Oviedo, Spain.
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Gómez
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Ribas-Aparicio
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Pélaez
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Rodicio
D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016364:Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000235:genetics*
D004251:DNA Transposable Elements