Effects of D-methionine-containing solution on tumor cell growth in vitro.
The effects of a nutrition therapy with D-methionine (Met)-containing solution were investigated in cell cultures of the AH109A cell line. The growth of AH109A hepatoma cells in culture media with D-Met-supplemented medium, L-Met-supplemented medium (control) and Met-free medium was compared. The D-Met-supplemented medium inhibited the cell growth to an extent similar to that manifested in the Met-free medium. The total free amino acid concentrations in the control medium decreased by approximately 40% on day 6 post-culture. However, the free amino acid concentrations in D-Met-supplemented and Met-free media did not change. Furthermore, alanine, which was not added to RPMI-1640, was detected in the control medium on day 6 post-culture. These results suggest the possibility of application of D-Met-containing solution to cancer patients receiving total parenteral nutrition.
Infusion Research Department, Hoechst Marion Roussel Ltd., Saitama, Japan.
T
T
Sasamura
A
A
Matsuda
Y
Y
Kokuba
D000596:Amino Acids / Q000032:analysis / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy; D008715:Methionine / Q000494:pharmacology*; D012996:Solutions; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D000970:Antineoplastic Agents; D003470:Culture Media; D012996:Solutions; D008715:Methionine