DNA vaccination favours memory rather than effector B cell responses.
Following priming and boosting of mice with a DNA vector pEE6DeltaS-hCGss expressing sequences encoding a transmembrane version of the beta-chain of human chorionic gonadotropin (hCGbeta), we failed to detect appreciable levels of specific antibody. However, subsequent challenge with hCG protein in Ribi adjuvant elicited a strong and rapid secondary immune response. This response was of comparable magnitude to that produced following priming, boosting and challenge with protein in adjuvant. Thus, DNA vaccination with this vector is as efficient in generating B cell memory as is conventional immunization, but the memory generation occurs in the absence of an overt effector response. Despite an overall similar level of specific antibody, the DNA-vaccinated mice produced hCG-specific antibodies biased towards IgG2a and IgG2b isotypes, whereas the protein-vaccinated mice produced higher levels of IgG1 antibodies. Both Th1 and Th2 cytokines (interferon-gamma (IFN-gamma) and IL-4) were lower in the spleens of the DNA-immunized animals compared with the protein-Ribi-immunized animals, possibly suggesting a different level of helper T cell response to the two different modes of immunization.
Medical Molecular Biology Unit, Department of Immunology, The Windeyer Institute for Medical Sciences, UCL, London, UK.
R
R
Laylor
N
N
Porakishvili
J B
JB
De Souza
J H
JH
Playfair
P J
PJ
Delves
T
T
Lund
D000276:Adjuvants, Immunologic; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000235:genetics / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006801:Humans; D007114:Immunization; D007117:Immunization, Secondary; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D018997:Chorionic Gonadotropin, beta Subunit, Human; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma