An optimized method for fatty acid analysis, including quantification of trans fatty acids, in human adipose tissue by gas-liquid chromatography.
Considering the need for a quick direct method for measurement of the fatty acid composition including trans isomers of human adipose tissue we have developed a procedure using gas-liquid chromatography (GLC) alone, which is thus suitable for validation of fatty acid status in epidemiological studies. Fatty acids ranging in carbon number from 12 to 22 and with 0-6 double bonds were resolved and identified by capillary column GLC with a temperature program starting at 150 degrees C. Following injection, the oven temperature was increased at a rate of 3 degrees C/min to 200 degrees C, then held constant for 25 min, and finally raised at 25 degrees C/min to 225 degrees C. The trans and cis isomers of 18:1 were well separated from each other, as shown by silver-ion thin-layer chromatography. Verification by standards showed that the trans 18:1 isomers with a double bond in position 12 or lower were separated from the cis 18:1 isomers with a double bond in position 6 or higher. As the adipose tissue samples contained only small amounts of the 13t-, 14t- and 15t-18:1 isomers and the 4c- and 5c-18:1 isomers the overlapping was found to be minimal. The GLC method may also be valuable for determining the fatty acid profiles including total trans in other tissues.
Department of Biochemistry and Nutrition, The Technical University of Denmark, Copenhagen. bysted@mimer.be.dtu.dk
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D000273:Adipose Tissue / Q000737:chemistry*; D000368:Aged; D002849:Chromatography, Gas / Q000379:methods* / Q000592:standards; D002855:Chromatography, Thin Layer / Q000379:methods* / Q000592:standards; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005260:Female; D006801:Humans; D007536:Isomerism; D008875:Middle Aged; D015203:Reproducibility of Results; D016622:Silver Staining; D013696:Temperature
D004952:Esters; D005227:Fatty Acids