Persistent activation of calcium-activated and calcium-independent protein kinase C in response to electrical afterdischarge from peptidergic neurons of aplysia.
The purpose of this work was to investigate the effects of electrical afterdischarge on protein kinase C (PKC) activity from bag cell neurons (BCNs) of Aplysia. Bilateral clusters of BCNs were divided: one cluster was stimulated to afterdischarge, the other was a control. Clusters were processed for PKC activity assay 5-120 min after electrical stimulation. Afterdischarge triggered a rapid and persistent increase in both calcium-activated and calcium-independent PKC activity.
Department of Physiology, UCLA School of Medicine, Room 53-231 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA. nwayne@mednet.ucla.edu
N L
NL
Wayne
W
W
Lee
Y J
YJ
Kim
D000818:Animals; D001048:Aplysia / Q000502:physiology*; D002118:Calcium / Q000502:physiology*; D004558:Electric Stimulation; D004594:Electrophysiology; D004789:Enzyme Activation; D009474:Neurons / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism*
D009479:Neuropeptides; D011493:Protein Kinase C; D002118:Calcium