Genomic organization, sequence and transcriptional regulation of the human CXCL 11(1) gene.
CXCL 11, encoded by the cDNA sequences designated beta-R1, H-174, or I-TAC, is a CXC chemokine ligand for CXCR3 and assumed to be involved in inflammatory diseases characterized by the presence of activated T-cells. We here describe the genomic organization (four exons interrupted by three introns of 585, 98 and 230 bp) and sequence including 960 bp from the immediate 5'-upstream region of the human CXCL 11 gene. Within the promoter region, consensus sequences for regulatory elements (ISRE, GAS, NF-kappaB) important for cytokine-induced gene transcription were identified. The effect of (pro)inflammatory cytokines on CXCL 11 mRNA expression in monocytic cell lines (THP-1, U937) and primary cultures of dermal fibroblasts and endothelial cells were examined using Northern blot analysis. For these cell types, IFN-gamma was a potent inducer of CXCL 11 transcription, which was synergistically enhanced by TNF-alpha.
Department of Dermatology, University Hospital Vrije Universiteit, Amsterdam/Leiden Institute for Immunology, 1081 HV, Amsterdam, Netherlands. cp.tensen@azvu.nl
C P
CP
Tensen
J
J
Flier
S S
SS
Rampersad
S
S
Sampat-Sardjoepersad
R J
RJ
Scheper
D M
DM
Boorsma
R
R
Willemze
D001483:Base Sequence; D002460:Cell Line; D054371:Chemokine CXCL11; D019743:Chemokines, CXC / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D015698:Genomic Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
C515202:CXCL11 protein, human; D054371:Chemokine CXCL11; D019743:Chemokines, CXC; D012333:RNA, Messenger