Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum.
Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis. We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.
Department of Molecular Microbiology and Immunology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
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Lobo
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Fujioka
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Aikawa
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Kumar
D000818:Animals; D048429:Cell Size; D002478:Cells, Cultured; D005260:Female; D017353:Gene Deletion; D015870:Gene Expression; D020043:Genes, Essential / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics; D008297:Male; D008854:Microscopy, Electron; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D010641:Phenotype; D010963:Plasmodium falciparum / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D011233:Precipitin Tests; D015800:Protozoan Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011995:Recombination, Genetic; D014170:Transformation, Genetic / Q000235:genetics; D014617:Vacuoles / Q000378:metabolism
D015800:Protozoan Proteins; D012333:RNA, Messenger; C069962:Pfg25-27 protein, Plasmodium falciparum