Analysis of cells in cerebrospinal fluid from patients with neurocysticercosis by means of flow cytometry.
The events of the cellular immune response in neurocysticercosis (NC) are not fully understood. Studies of the CD3, CD3/CD4, CD3/CD8, CD45/CD19, and CD45/CD56 molecules and activation-related CD69 molecule in cells from the cerebrospinal fluid (CSF) and peripheral blood (PB) of patients with NC may provide a better elucidation of the inflammatory and immunological events occurring in this disease. Seven patients with NC and 3 individuals with other disorders were evaluated by a three-color flow cytometric method. CD69 was detected in a higher percentage of cells in all CSF samples from patients, but not in PB or CSF from the control group. The percentage of CD3+ cells did not differ significantly in CSF and PB cells from patients and controls. The predominance of CD3+CD8+ cells was observed in CSF from one patient and in PB from 2 patients, who were in stage III of the disease (inflammatory process). The percentage of CD45+CD19+ cells was higher in CSF than in PB from patients who presented anti-cysticercus antibodies in CSF. The percentage of CD45+CD56+ cells in CSF was higher than in PB, but this rate was similar to reference values reported by other authors. Our data suggest that the cytometric method applied to a larger number of CSF samples may provide a better understanding of the cell-mediated immune response involved in NC.
Bueno
E C
EC
Faculty of Pharmaceutical Sciences, University of São Paulo, SP, Brazil. ecbueno@usp.br
Vaz
A J
AJ
Oliveira
C A
CA
Machado
L R
LR
Livramento
J A
JA
Mielli
S R
SR
Ueda
M
M
D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology; D020019:Neurocysticercosis / Q000134:cerebrospinal fluid* / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology
D015703:Antigens, CD