Molecular analysis of a spontaneous dystrophin 'knockout' dog.
We have determined the molecular basis for skeletal myopathy and dilated cardiomyopathy in two male German short-haired pointer (GSHP) littermates. Analysis of skeletal muscle demonstrated a complete absence of dystrophin on Western blot analysis. PCR analysis of genomic DNA revealed a deletion encompassing the entire dystrophin gene. Molecular cytogenetic analysis of lymphocytes from the dam and both dystrophic pups confirmed a visible deletion in the p21 region of the affected canine X chromosome. Utrophin is up-regulated in the skeletal muscle, but does not appear to ameliorate the dystrophic canine phenotype. This new canine model should further our understanding of the physiological and biochemical processes in Duchenne muscular dystrophy.
Schatzberg
S J
SJ
College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA. scott_schatzberg@ncsu.edu
Olby
N J
NJ
Breen
M
M
Anderson
L V
LV
Langford
C F
CF
Dickens
H F
HF
Wilton
S D
SD
Zeiss
C J
CJ
Binns
M M
MM
Kornegay
J N
JN
Morris
G E
GE
Sharp
N J
NJ
D000818:Animals; D001706:Biopsy; D015153:Blotting, Western; D002872:Chromosome Deletion; D003402:Creatine Kinase / Q000097:blood; D004247:DNA / Q000235:genetics; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D016189:Dystrophin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009137:Muscular Dystrophy, Animal / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D016133:Polymerase Chain Reaction; D014960:X Chromosome / Q000235:genetics
D016189:Dystrophin; D004247:DNA; D003402:Creatine Kinase