Establishing and characterizing a CD30-positive cell line harboring HHV-8 from a primary effusion lymphoma.
Primary effusion lymphoma (PEL, or body-cavity-based lymphoma [BCBL]) is a new subtype of non-Hodgkin's lymphoma in which tumor cells locate in the body cavity exclusively. PEL/BCBL is widely accepted as one of the neoplastic complications of AIDS, associated mostly with human herpesvirus 8 (HHV-8/Kaposi's sarcoma-associated herpesvirus [KSHV]) and Epstein-Barr virus (EBV). We established and characterized a PEL cell line named TY-1 from a 47-year-old patient with AIDS. TY-1 exhibits indeterminate immunophenotype, expressing CD45 and CD30 cell surface antigens but not expressing B- or T-cell markers. Cytogenetic analysis revealed the representative karyotype of 50,XYq-,+7,+8,+11,+15. Southern blot analysis demonstrated HHV-8 and EBV genomes in the original tumor cells obtained from the pericardial effusion, while HHV-8 but not EBV was detected in TY-1 using PCR or Southern blot analysis. Tetradecanylphorbol acetate treatment induced some TY-1 cells to proceed to the reproductive phase. This cell line may be an useful tool for research on PEL and HHV-8.
Department of Pathology, Institute of Medical Science, University of Tokyo, Japan.
H
H
Katano
Y
Y
Hoshino
Y
Y
Morishita
T
T
Nakamura
H
H
Satoh
A
A
Iwamoto
B
B
Herndier
S
S
Mori
D017809:Fatal Outcome; D015870:Gene Expression; D015321:Gene Rearrangement; D016679:Genome, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D006801:Humans; D016130:Immunophenotyping; D017403:In Situ Hybridization; D007621:Karyotyping; D017730:Ki-1 Antigen / Q000032:analysis*; D008223:Lymphoma / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology / Q000821:virology
D017730:Ki-1 Antigen; D012333:RNA, Messenger; D012367:RNA, Viral