Bifunctional phosphoramidite reagents for the introduction of histidyl and dihistidyl residues into oligonucleotides.
The synthesis and characterization of reagents for the incorporation of histidyl residues into oligonucleotides by automated chemical synthesis is described. Automated oligonucleotide synthesis utilizing a bifunctional reagent for the incorporation of a dihistidyl residue into oligonucleotides is described. Oligonucleotides incorporating one to three dihistidyl residues were prepared and characterized. The interaction of these oligonucleotides with a metal chelating IMAC matrix was explored.
Smith
T H
TH
Nucleic Acids Chemistry Division, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, California 94303, USA. toms@clontech.com
LaTour
J V
JV
Bochkariov
D
D
Chaga
G
G
Nelson
P S
PS
D000577:Amides / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006639:Histidine / Q000737:chemistry*; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry*
D000577:Amides; D002614:Chelating Agents; D007202:Indicators and Reagents; D009841:Oligonucleotides; D010756:Phosphoric Acids; D006639:Histidine; C011067:phosphoramidic acid