Bovine CD18 identified as a species specific receptor for Pasteurella haemolytica leukotoxin.
Ligand blotting of Pasteurella haemolytica leukotoxin (LKT) susceptible BL3 bovine lymphoma cell membranes with LKT detected two putative receptors with Mr of 95 and 100 kDa, whereas no LKT binding to membrane proteins was detected for LKT non-susceptible human leukemic cells. Anti-bovine CD18 and CD11a/CD18 mAb recognized 95 and 100kDa bands from BL3 cell membranes. CD18 isolated from BL3 cell membranes bound LKT. Pre-incubation of BL3 cells with anti-bovine CD18 or CD11a/CD18 mAb caused partial inhibition of LKT-induced leukolysis. Therefore, we propose that bovine CD18 acts as a species-specific leukocyte receptor for P. haemolytica LKT.
Department of Anatomy, Pathology, and Pharmacology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
J
J
Li
K D
KD
Clinkenbeard
J W
JW
Ritchey
D000818:Animals; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D018821:CD18 Antigens / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005098:Exotoxins / Q000276:immunology*; D018922:HL-60 Cells; D006801:Humans; D016978:Mannheimia haemolytica / Q000276:immunology*; D010326:Pasteurella Infections / Q000662:veterinary; D011233:Precipitin Tests / Q000662:veterinary; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D018821:CD18 Antigens; D005098:Exotoxins; C018579:leukotoxin