An adhesin of the yeast pathogen Candida glabrata mediating adherence to human epithelial cells.
Candida glabrata is an important fungal pathogen of humans that is responsible for about 15 percent of mucosal and systemic candidiasis. Candida glabrata adhered avidly to human epithelial cells in culture. By means of a genetic approach and a strategy allowing parallel screening of mutants, it was possible to clone a lectin from a Candida species. Deletion of this adhesin reduced adherence of C. glabrata to human epithelial cells by 95 percent. The adhesin, encoded by the EPA1 gene, is likely a glucan-cross-linked cell-wall protein and binds to host-cell carbohydrate, specifically recognizing asialo-lactosyl-containing carbohydrates.
Cormack
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BP
Department of Microbiology and Immunology, Stanford University School of Medicine, Fairchild D039, 299 Campus Drive, Stanford, CA 94305-5124, USA. bcormack@jhmi.edu
Ghori
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N
Falkow
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S
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002175:Candida / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology*; D005260:Female; D005656:Fungal Proteins; D005800:Genes, Fungal; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured; D014774:Virulence / Q000235:genetics
D002241:Carbohydrates; D005656:Fungal Proteins; D037102:Lectins; D008024:Ligands; C120505:epithelial adhesin 1, Candida glabrata; D002118:Calcium