Effects of photochemically immobilized polymer coatings on protein adsorption, cell adhesion, and the foreign body reaction to silicone rubber.
Photochemical immobilization technology was utilized to covalently couple polymers to silicone rubber either at multiple points along a polymer backbone or at the endpoint of an amphiphilic chain. The coating variants then were tested in vitro and in vivo for improvement of desired responses compared to uncoated silicone rubber. All coating variants suppressed the adsorption of fibrinogen and immunoglobulin G, and most also inhibited fibroblast growth by 90-99%. None of the coating variants inhibited monocyte or neutrophil adhesion in vitro. However, the surfaces that supported the highest levels of monocyte adhesion also elicited the lowest secretion of pro-inflammatory cytokines. None of the materials elicited a strong inflammatory response or significantly (p< 0.05) reduced the thickness of the fibrous capsule when implanted subcutaneously in rats. Overall, the most passivating coating variant was an endpoint immobilized polypeptide that reduced protein adsorption, inhibited fibroblast growth by 90%, elicited low cytokine secretion from monocytes, and reduced fibrous encapsulation by 33%. In general, although some coating variants modified the adsorption of proteins and the behavior of leukocytes or fibroblasts in vitro, none abolished the development of a fibrous capsule in vivo.
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
K M
KM
DeFife
M S
MS
Shive
K M
KM
Hagen
D L
DL
Clapper
J M
JM
Anderson
D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D001577:Benzophenones / Q000528:radiation effects*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005260:Female; D005340:Fibrinogen / Q000737:chemistry; D005355:Fibrosis; D005549:Foreign-Body Reaction / Q000209:etiology*; D006820:Hyaluronic Acid / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry; D010777:Photochemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011205:Povidone / Q000737:chemistry; D019736:Prostheses and Implants; D019919:Prosthesis Implantation / Q000009:adverse effects; D011506:Proteins / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012826:Silicone Elastomers / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001577:Benzophenones; D020099:Coated Materials, Biocompatible; D005340:Fibrinogen; D006820:Hyaluronic Acid; D007074:Immunoglobulin G; D009842:Oligopeptides; D011092:Polyethylene Glycols; D011108:Polymers; D011205:Povidone; D011506:Proteins; D012826:Silicone Elastomers; D000099166:Polyacrylamides; C102077:glycyl-valyl-glycyl-valyl-proline; C058818:4-benzoylbenzoic acid; C028210:monomethoxypolyethylene glycol