Synthesis and nucleosome structure of DNA containing a UV photoproduct at a specific site.
A strategy was developed to assemble nucleosomes specifically damaged at only one site and one structural orientation. The most prevalent UV photoproduct, a cis-syn cyclobutane thymine dimer (cs CTD), was chemically synthesized and incorporated into a 30 base oligonucleotide harboring the glucocorticoid hormone response element. This oligonucleotide was assembled into a 165 base pair double stranded DNA molecule with nucleosome positioning elements on each side of the cs CTD-containing insert. Proton NMR verified that the synthetic photoproduct is the cis-syn stereoisomer of the CTD. Moreover, two different pyrimidine dimer-specific endonucleases cut approximately 90% of the dsDNA molecules. This cleavage is completely reversed by photoreactivation with E. coli UV photolyase, further demonstrating the correct stereochemistry of the photoproduct. Nucleosomes were reconstituted by histone octamer exchange from chicken erythocyte core particles, and contained a unique translational and rotational setting of the insert on the histone surface. Hydroxyl radical footprinting demonstrates that the minor groove at the cs CTD is positioned away from the histone surface about 5 bases from the nucleosome dyad. Competitive gel-shift analysis indicates there is a small increase in histone binding energy required for the damaged fragment (DeltaDeltaG approximately 0.15 kcal/mol), which does not prevent complete nucleosome loading under our conditions. Finally, folding of the synthetic DNA into nucleosomes dramatically inhibits cleavage at the cs CTD by T4 endonuclease V and photoreversal by UV photolyase. Thus, specifically damaged nucleosomes can be experimentally designed for in vitro DNA repair studies.
Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4660, USA.
J V
JV
Kosmoski
M J
MJ
Smerdon
D017122:Bacteriophage T4 / Q000201:enzymology; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000138:chemical synthesis / Q000378:metabolism; D013873:Thionucleotides / Q000138:chemical synthesis / Q000378:metabolism; D013941:Thymine / Q000138:chemical synthesis / Q000378:metabolism; D014466:Ultraviolet Rays; D014764:Viral Proteins
D009707:Nucleosomes; D011740:Pyrimidine Dimers; D013873:Thionucleotides; D014764:Viral Proteins; D004247:DNA; D004706:Endodeoxyribonucleases; C470698:endonuclease V, phage T4; D043224:Deoxyribonuclease (Pyrimidine Dimer); D013941:Thymine