Diversity of HLA-B17 alleles and haplotypes in East Asians and a novel Cw6 allele (Cw*0604) associated with B*5701.
The distribution of HLA-B17 alleles and their association with HLA-A, -C and -DRB1 alleles were investigated in seven East Asian populations Japanese, South Korean, Chinese-Korean, Man, Northern Han, Mongolian and Buryat populations). The B17 alleles were identified from genomic DNA using group-specific polymerase chain reaction (PCR) followed by hybridization with sequence-specific oligonucleotide probes (SSOP). In all of these East Asian populations, except Japanese and Chinese-Koreans, B*5701 was detected and strongly associated with A*0101, Cw*0602 and DRB1*0701. In contrast, B*5801 was detected in all the seven populations and strongly associated with A*3303, Cw*0302, DRB1*0301 and DRB1*1302. The A*3303-Cw*0302-B*5801-DRB1*1302 haplotype was observed in South Korean, Chinese-Korean, Buryat and Japanese populations, while A*3303-Cw*0302-B*5801-DRB1*0301 was predominantly observed in the Mongolian population. A similar haplotype, A*0101-Cw*0302-B*5801-DRB1*1302, was observed in the Buryat population. A novel Cw6 allele, Cw*0604, was identified in the Man population. This Cw allele was observed on the haplotype A*0101-B*5701-DRB1*0701. Thus, we confirmed, at the sequence level, that the common haplotypes carrying B*5701 and B*5801 have been conserved and shared in East Asian populations.
Japanese Red Cross Central Blood Center, Tokyo.
T
T
Inoue
A
A
Ogawa
K
K
Tokunaga
Y
Y
Ishikawa
K
K
Kashiwase
H
H
Tanaka
M H
MH
Park
G J
GJ
Jia
N O
NO
Chimge
E W
EW
Sideltseva
T
T
Akaza
K
K
Tadokoro
T
T
Takahashi
T
T
Juji
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005787:Gene Frequency; D014644:Genetic Variation / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics* / Q000302:isolation & purification; D006239:Haplotypes / Q000276:immunology*; D006801:Humans; D007564:Japan / Q000208:ethnology; D007723:Korea / Q000208:ethnology; D008969:Molecular Sequence Data
D015235:HLA-B Antigens; C037449:HLA-B17 antigen; C103707:HLA-B57 antigen; D015236:HLA-C Antigens; C528994:HLA-C*06 antigen