In vitro interaction of serum protein with circulating DNA of lung cancer patient.
Circulating DNA and oncoproteins can be extracted from serum or plasma of cancer patients. In this study, using gel retardation analysis we observed circulating DNA obtained from plasma of a lung cancer patient complexed with serum proteins. p53 was identified by immunoblotting as one of the proteins present in the complex. Our finding suggests that the same interaction observed between p53 and DNA in intact cells occurs in serum of lung cancer patient. As far as we know this is the first evidence for such finding.
Laboratório de Controle da Expressão Genica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, RJ, Brazil.
M T
MT
Kawamura
M E
ME
Paschoal
M D
MD
da Costa Carvalho
D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D018288:Carcinoma, Small Cell / Q000097:blood* / Q000473:pathology; D004247:DNA / Q000097:blood / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology; D009698:Nucleoproteins / Q000032:analysis; D011485:Protein Binding; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D001798:Blood Proteins; D009698:Nucleoproteins; D016159:Tumor Suppressor Protein p53; D004247:DNA