Oxidative DNA base damage in cancerous tissues of patients undergoing brachytherapy.
This aim of this study was to measure the typical free radical-induced products of DNA bases in cellular DNA of cervical cancer tissues directly irradiated by applying brachytherapy to the patients. Significant increases in the amounts of modified bases over the control level were observed in the samples isolated after irradiation for all patients. These increases differed among patients and among products. The repair capacity and/or the amount of hypoxic cells inside the tumor may account for the different levels of modified bases. It is possible that the observed variabilities may account for the differences in clinical responses to brachytherapy.
Department of Clinical Biochemistry, University School of Medical Science, Bydgoszcz, Poland.
M
M
Foksinski
P
P
Jaruga
R
R
Makarewicz
R
R
Olinski
D000225:Adenine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D001918:Brachytherapy / Q000009:adverse effects*; D003596:Cytosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D010084:Oxidation-Reduction; D011743:Pyrimidines / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D014498:Uracil / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000532:radiotherapy
D004273:DNA, Neoplasm; D011743:Pyrimidines; C002992:8-hydroxyadenine; C045975:5-hydroxyuracil; C039544:4,6-diamino-5-N-formamidopyrimidine; C024829:8-hydroxyguanine; D014498:Uracil; D006147:Guanine; C017400:5-hydroxycytosine; D003596:Cytosine; D000225:Adenine