Production and purification of Clostridium botulinum type C and D neurotoxin.
Neurotoxins of Clostridium botulinum are needed in basic neurologic research, but as therapeutic agent for certain neuromuscular disorders like strabism as well. A method for the production and purification of botulinum neurotoxins C and D is reported using a two-step hollow-fiber cross flow filtration and a newly developed chromatographic purification procedure. Hollow-fiber filtration proved to be a rapid and safe concentration and pre-purification step, which can easily be scaled up. The chromatographic purification included hydrophobic interaction, anion exchange and size exclusion chromatography runs. Botulinum neurotoxins C and D could be recovered with an overall yield of 12.6% and 10.6%, respectively. A specific toxicity of 1.86 x 10(7) minimal lethal dose mg(-1) (type C) and 5.26 x 10(7) minimal lethal dose mg(-1) (type D) was determined in the mouse bioassay.
Gessler
F
F
Institute for Crop and Animal Production in the Tropics, Georg August University, Göttingen, Germany. fgessle@gwdg.de
Böhnel
H
H
D000818:Animals; D001905:Botulinum Toxins / Q000096:biosynthesis* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D003014:Clostridium botulinum / Q000378:metabolism*; D005374:Filtration; D051379:Mice; D009498:Neurotoxins / Q000096:biosynthesis / Q000302:isolation & purification
D009498:Neurotoxins; C096325:botulinum toxin type D; D001905:Botulinum Toxins; C096324:botulinum toxin type C