Duodenal neurons provide nicotinic fast synaptic input to sphincter of Oddi neurons in guinea pig.
We have investigated the existence of neural connections between the duodenum and the sphincter of Oddi (SO). Stimulation of duodenal myenteric fiber bundles elicited synaptic responses in SO neurons, which included nicotinic fast excitatory postsynaptic potentials (EPSPs), slow EPSPs, and alpha(2)-adrenoreceptor-mediated inhibitory postsynaptic potentials. After 48 h in organ culture, when extrinsic fibers had diminished, only the fast EPSPs persisted. Duodenal mucosal stimulation also elicited nicotinic fast EPSPs in SO neurons. There was no association between the SO neurons that received duodenal input and their chemical coding. A reciprocal projection also exists from the SO to the duodenum. In acute and cultured preparations, duodenal myenteric stimulation caused antidromic responses in 20% of SO neurons. Furthermore, 45.6 +/- 10.5 neurons in SO ganglia were retrogradely labeled from dye application sites in the duodenum. It is proposed that bidirectional neural communication occurs between the duodenum and the SO and that duodenal neurons provide excitatory fast synaptic input to SO neurons through a reflex that can be activated at the duodenal mucosa.
Mawe
G M
GM
Department of Anatomy and Neurobiology, University of Vermont, Burlington, Vermont 05405, USA. gmawe@zoo.uvm.edu
Kennedy
A L
AL
D000344:Afferent Pathways / Q000166:cytology / Q000502:physiology; D000818:Animals; D003714:Denervation; D004386:Duodenum / Q000294:innervation*; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005260:Female; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000502:physiology; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000502:physiology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D009538:Nicotine / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009924:Organ Culture Techniques; D009803:Sphincter of Oddi / Q000294:innervation*; D013569:Synapses / Q000502:physiology*; D013997:Time Factors
D009538:Nicotine; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase