A perspective of gene therapy in the glaucomas.
Gene therapy in the anterior and posterior segment tissues may have the potential to favorably influence aqueous hydrodynamics and retinal ganglion cell biology, thereby preventing, delaying, or minimizing glaucomatous damage to the optic nerve. We demonstrated the feasibility of using a herpes viral vector (ribonucleotide reductase defective HSV-1, hrR3) to deliver the lacZ reporter gene to living cat and rat eyes. Cats received injections into the anterior chamber and rats into the vitreous cavity. In cats, lacZ expression was detectable at 1 to 2 days in the anterior outer portion of the ciliary muscle and the lining of the intertrabecular spaces of the corneoscleral and uveal meshwork. Rat eyes showed lacZ expression in the retinal pigment epithelium and photoreceptor outer segments 2 days after injection.
Kaufman
P L
PL
Department of Ophthalmology, University of Wisconsin, Madison 53792-3220, USA.
Jia
W W
WW
Tan
J
J
Chen
Z
Z
Gabelt
B T
BT
Booth
V
V
Tufaro
F
F
Cynader
M
M
D000818:Animals; D000869:Anterior Eye Segment / Q000378:metabolism / Q000473:pathology; D002415:Cats; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression / Q000187:drug effects; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy; D005822:Genetic Vectors; D005901:Glaucoma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D010786:Photoreceptor Cells / Q000378:metabolism / Q000473:pathology; D010857:Pigment Epithelium of Eye / Q000378:metabolism / Q000473:pathology; D051381:Rats; D020318:Rats, Long-Evans; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004279:DNA, Viral; D001616:beta-Galactosidase