Immunogenicity of various presentation forms of PorA outer membrane protein of Neisseria meningitidis in mice.
In this study we compare different vaccine formulations containing meningococcal PorA outer membrane protein; purified PorA, outer membrane vesicles (OMV) and immune-stimulating complexes (iscom). Bactericidal antibodies could be generated by the OMV and iscom formulation but not with purified PorA using either A1PO4 or Quil-A as adjuvant. OMV and iscom formulations revealed similar immunogenicity when tested in a dose response manner, with respect to bactericidal as well as OMV-binding antibodies. The anti-OMV IgG subclass response induced by PorA in OMV formulation was found in all subclasses IgG1, IgG2a, IgG2b, IgG3. OMP-iscoms induced very high IgG1 anti-OMV antibodies but almost no IgG3 response. Also, OMP-iscoms appeared to be a potent inducer of antibodies directed against linear peptides corresponding to surface exposed loops of PorA. In addition, iscoms as well as purified PorA with Quil-A as adjuvant (but not with A1PO4) induced high levels of antibodies against purified PorA. In summary, in addition to the OMV formulation, only iscoms containing PorA are able to generate an anamnestic and bactericidal antibody response.
Laboratory for Vaccine development and Immune Mechanisms, National Institute of Public Health and Environment, Bilthoven, The Netherlands.
C C
CC
Peeters
I J
IJ
Claassen
M
M
Schuller
G F
GF
Kersten
E M
EM
van der Voort
J T
JT
Poolman
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D001770:Blood Bactericidal Activity; D005260:Female; D016882:ISCOMs / Q000008:administration & dosage; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000276:immunology*; D018272:Porins / Q000008:administration & dosage / Q000276:immunology*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D016882:ISCOMs; D007106:Immune Sera; D018272:Porins; C057699:porin protein, Neisseria