Alteration of the repressor activity of MarR, the negative regulator of the Escherichia coli marRAB locus, by multiple chemicals in vitro.
MarR negatively regulates expression of the multiple antibiotic resistance operon (marRAB) in Escherichia coli. In this study, it was demonstrated that sodium salicylate, plumbagin, 2, 4-dinitrophenol, and menadione-inducers of the marRAB operon in whole cells-all interfered with the repressor activity of MarR in vitro. It is proposed that these compounds can interact directly with MarR to affect its repressor activity.
Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
M N
MN
Alekshun
S B
SB
Levy
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D009285:Naphthoquinones / Q000494:pharmacology; D009876:Operon / Q000187:drug effects; D010957:Plasmids; D012097:Repressor Proteins / Q000378:metabolism*; D015183:Restriction Mapping; D012980:Sodium Salicylate / Q000494:pharmacology; D014812:Vitamin K / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C080004:MarR protein, E coli; D009285:Naphthoquinones; D012097:Repressor Proteins; D014812:Vitamin K; D019297:2,4-Dinitrophenol; D012980:Sodium Salicylate; C014758:plumbagin