Preparation and functional properties of monoclonal antibodies to human, mouse and rat OX-2.
We have prepared mouse and rat hybridomas to a 43-kDa molecule expressed in the thymus, on a subpopulation of dendritic cells, and in the brain, in mammalian tissue derived from mouse, rat and human. Using CHO cells transiently transfected with adenovirus vector(s) expressing a cDNA construct for the relevant OX-2 gene, we show these monoclonal antibodies (Mabs) detect a molecule encoded by this construct (rat OX-2 (rOX-2), mouse OX-2 (mOX-2) and human OX-2 (huOX-2), respectively). Furthermore, at least some of the anti-rat Mabs detect determinants expressed on the murine OX-2 molecule, as we predicted in an earlier publication. Previous studies have implied that this molecule might serve an important role in regulation of cell signaling for cytokine production. Using one-way mixed leukocyte reactions we show that when cells are cultured in the presence of the species-specific Mab, cytokine production becomes polarized 'away from' type-2 cytokine production, with preferentially increased expression of type-1 cytokine production.
Ragheb
R
R
Transplant Research Division, The Toronto Hospital, ON, Canada.
Abrahams
S
S
Beecroft
R
R
Hu
J
J
Ni
J
J
Ramakrishna
V
V
Yu
G
G
Gorczynski
R M
RM
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D007378:Interleukins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; C042739:antigens, CD200