title: Identification of novel immunogenic Mycobacterium tuberculosis peptides that stimulate mononuclear cells from immune donors.
abstract: Proteins which are secreted or associated with the cell envelope of Mycobacterium tuberculosis may contain protective T-cell epitopes. Prior to this study, a recombinant clone bank of enzymatically active M. tuberculosis-alkaline phosphatase fusions, were screened for immunogenicity in a murine T-cell model. Five of these were selected for further study, and the IFN-gamma secretion and proliferation of human PBMC from purified protein derivative- (PPD)-positive and PPD-negative donors were measured in response to oligopeptides, Mtb-PhoA fusions and one full-length protein. Epitopes from four of the five selected antigens were immunoreactive in the human model and corresponded to cytochrome d ubiquinol oxidase, cytochrome c oxidase subunit II, MTV005.02 and MTV033.08. Thus, this strategy identified novel human immunogenic peptides as possible candidates for a subunit vaccine.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Canada. jmoran@uvic.ca
authors.forename: A J
authors.initials: AJ
authors.lastname: Moran
authors.forename: J L
authors.initials: JL
authors.lastname: Doran
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: J D
authors.initials: JD
authors.lastname: Treit
authors.forename: P
authors.initials: P
authors.lastname: Ekpo
authors.forename: V J
authors.initials: VJ
authors.lastname: Kerr
authors.forename: A D
authors.initials: AD
authors.lastname: Roberts
authors.forename: I M
authors.initials: IM
authors.lastname: Orme
authors.forename: S
authors.initials: S
authors.lastname: Galant
authors.forename: S R
authors.initials: SR
authors.lastname: Ress
authors.forename: F E
authors.initials: FE
authors.lastname: Nano
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000276:immunology; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma; D003576:Electron Transport Complex IV; D000469:Alkaline Phosphatase