Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer).
The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5.0 (SE = 0.2) and the mean heterozygosity per marker was 0.61 (SE = 0.03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.
van Hooft
W F
WF
Wageningen Agricultural University, Department of Environmental Sciences, Wageningen, The Netherlands.
Hanotte
O
O
Wenink
P W
PW
Groen
A F
AF
Sugimoto
Y
Y
Prins
H H
HH
Teale
A
A
D000483:Alleles; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005260:Female; D005828:Genetics, Population; D006579:Heterozygote; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity