Cohesin Rec8 is required for reductional chromosome segregation at meiosis.
When cells exit from mitotic cell division, their sister chromatids lose cohesion and separate to opposite poles of the dividing cell, resulting in equational chromosome segregation. In contrast, the reductional segregation of the first stage of meiotic cell division (meiosis I) requires that sister chromatids remain associated through their centromeres and move together to the same pole. Centromeric cohesion is lost as cells exit from meiosis II and sister chromatids can then separate. The fission yeast cohesin protein Rec8 is specific to and required for meiosis. Here we show that Rec8 appears in the centromeres and adjacent chromosome arms during the pre-meiotic S phase. Centromeric Rec8 persists throughout meiosis I and disappears at anaphase of meiosis II. When the rec8 gene is deleted, sister chromatids separate at meiosis I, resulting in equational rather than reductional chromosome segregation. We propose that the persistence of Rec8 at centromeres during meiosis I maintains sister-chromatid cohesion, and that its presence in the centromere-adjacent regions orients the kinetochores so that sister chromatids move to the same pole. This results in the reductional pattern of chromosome segregation necessary to reduce a diploid zygote to haploid gametes.
Watanabe
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Y
Cell Cycle Laboratory, Imperial Cancer Research Fund, London, UK. ywatanab@ims.u-tokyo.ac.jp
Nurse
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P
D018797:Cell Cycle Proteins; D002503:Centromere / Q000502:physiology; D020090:Chromosome Segregation / Q000502:physiology*; D015825:Chromosomes, Fungal / Q000502:physiology; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins; D006388:Hemagglutinins / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008540:Meiosis / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins; D013172:Spores, Fungal; D000097722:Cohesins
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins; D006388:Hemagglutinins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; C092957:Rad21 protein, S pombe; C077081:REC8 protein, S pombe; D000097722:Cohesins