[Roles of platelets and neutrophils in intimal thickening following endothelial injury].
We investigated the roles of platelets and neutrophils in intimal thickening following endothelial injury of the mouse femoral artery. The photochemical reaction between systemically administered Rose Bengal and transluminal green light produced endothelial injury followed by platelet adhesion and aggregation at the site of photochemical reaction. Further, 3 days after the injury, numerous neutrophils adhered to the damaged vessel wall. Three weeks after the injury, intimal thickening was produced at the irradiated site. A glycoprotein (GP) IIb/IIIa inhibitor, which strongly inhibits platelet aggregation, was administered orally for 3 weeks. In a separate experiment, myo-inositol hexaphosphate (phytic acid), which inhibits neutrophil adhesion to the damaged vessel wall due to blocking P-selectin, was administered intravenously. Phytic acid and a GP IIb/IIIa inhibitor inhibited intimal thickening. Thus, both neutrophils and platelets may play a key role in intimal thickening following endothelial injury in this model.
Umemura
K
K
Department of Pharmacology, Hamamatsu University School of Medicine.
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D004730:Endothelium, Vascular / Q000473:pathology*; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D017539:Tunica Intima / Q000473:pathology*