title: Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ.
abstract: Escherichia coli osmosensor EnvZ is a protein histidine kinase that plays a central role in osmoregulation, a cellular adaptation process involving the His-Asp phosphorelay signal transduction system. Dimerization of the transmembrane protein is essential for its autophosphorylation and phosphorelay signal transduction functions. Here we present the NMR-derived structure of the homodimeric core domain (residues 223-289) of EnvZ that includes His 243, the site of autophosphorylation and phosphate transfer reactions. The structure comprises a four-helix bundle formed by two identical helix-turn-helix subunits, revealing the molecular assembly of two active sites within the dimeric kinase.
authors.affiliation: Center for Tsukuba Advanced Research Alliance and Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Tomomori
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: R
authors.initials: R
authors.lastname: Dutta
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.forename: S K
authors.initials: SK
authors.lastname: Saha
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: R
authors.initials: R
authors.lastname: Ishima
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: K I
authors.initials: KI
authors.lastname: Tong
authors.forename: H
authors.initials: H
authors.lastname: Kurokawa
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.forename: M
authors.initials: M
authors.lastname: Inouye
authors.forename: M
authors.initials: M
authors.lastname: Ikura
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012996:Solutions
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D012996:Solutions; C057843:envZ protein, E coli