Neuron-targeted gene transfer by adenovirus carrying neural-restrictive silencer element.
Adenovirus transfers genes to a wide range of cell types, but its application to neurons has been hampered by its reduced efficiency of infection as compared with that for glia. To achieve neuron-targeted gene transfer, we have produced an adenovirus carrying the reporter lacZ gene driven by the SCG10 minimum promoter containing the neural-restrictive silencer element (NRSE), which element selectively represses the transcription of genes in non-neuronal cells. When rat hippocampal slice cultures were infected with NRSE-bearing adenovirus, beta-galactosidase-positive cells were mostly pyramidal and granular neurons, whereas infection with virus carrying a mutated NRSE resulted in beta-galactosidase expression in both neurons and glia. The results suggest that the adenovirus carrying NRSE to be a useful tool for neurontargeted gene transfer.
Miyaguchi
K
K
Laboratory of Neurobiology, NINDS, NIH, Bethesda, MD 20892, USA.
Maeda
Y
Y
Kojima
T
T
Setoguchi
Y
Y
Mori
N
N
D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000201:enzymology; D000818:Animals; D018014:Gene Transfer Techniques; D066298:In Vitro Techniques; D009154:Mutation / Q000502:physiology; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D017966:Pyramidal Cells / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000235:genetics*; D014162:Transfection / Q000502:physiology; D001616:beta-Galactosidase / Q000378:metabolism
D012097:Repressor Proteins; D001616:beta-Galactosidase