Triple-resonance experiments for correlation of H5 and exchangeable pyrimidine base hydrogens in (13)C,(15)N-labeled RNA.
Triple-resonance two-dimensional H5(C5C4N)H experiments are described that provide through-bond H5 to imino/amino connectivities in uridines and cytidines in (13)C, (15)N-labeled RNAs. The experiments employ selective INEPT steps for transferring magnetization from the H5 hydrogens through the intervening C5, C4, and N3/N4 nuclei to the imino/amino hydrogens. The improved sensitivity of these experiments for assignments in a large 43-nucleotide RNA is demonstrated.
Wöhnert
J
J
Abteilung für Molekulare Biophysik/NMR-Spektroskopie, Institut für Molekulare Biotechnologie e.V., Postfach 100813, Jena, D-07708, Germany.
Ramachandran
R
R
Görlach
M
M
Brown
L R
LR
D002247:Carbon Isotopes; D003562:Cytidine / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D011741:Pyrimidine Nucleosides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D014529:Uridine / Q000737:chemistry
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011741:Pyrimidine Nucleosides; D003562:Cytidine; D012313:RNA; D006859:Hydrogen; D014529:Uridine