title: Phosphorylation of anti-HIV nucleoside analogs by nucleoside diphosphate kinase.
abstract: The reaction of NDP kinase with antiviral nucleoside triphosphates used in antiviral therapies was studied at the presteady state by fluorescence stopped-flow and compared with the steady-state parameters. The affinity of the analogs was determined by fluorescence titration of a mutated enzyme with an inserted Trp in the binding site. The lack of the 3' hydroxyl in analogs is shown to decrease the kcat more than the KD.
authors.affiliation: Unité de Régulation Enzymatique des Activités Cellulaires, CNRS URA1773, Institut Pasteur, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Schneider
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: O
authors.initials: O
authors.lastname: Sellam
authors.forename: R
authors.initials: R
authors.lastname: Sarfati
authors.forename: J
authors.initials: J
authors.lastname: Janin
authors.forename: M
authors.initials: M
authors.lastname: Véron
authors.forename: D
authors.initials: D
authors.lastname: Deville-Bonne
mesh_terms: D019380:Anti-HIV Agents / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D009701:Nucleoside-Diphosphate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009705:Nucleosides / Q000378:metabolism*; D010766:Phosphorylation; D013379:Substrate Specificity
chemical_list: D019380:Anti-HIV Agents; D004791:Enzyme Inhibitors; D009705:Nucleosides; D009701:Nucleoside-Diphosphate Kinase