Isolation and characterization of the actin gene from the cellulolytic fungus Humicola grisea and analysis of transcription levels of actin and cellulase genes.
An actin gene was isolated from the cellulolytic fungus Humicola grisea. The gene structure, which has 5 introns in the coding region, is similar to those of the so far cloned fungal actin genes. But there are some differences in intron sizes and codon usage. Transcription levels of actin and cellulase genes were also investigated.
Takashima
S
S
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Nakamura
A
A
Hidaka
M
M
Masaki
H
H
Uozumi
T
T
D020121:5' Untranslated Regions / Q000235:genetics; D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004578:Electron Spin Resonance Spectroscopy; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D014158:Transcription, Genetic / Q000235:genetics*
D020121:5' Untranslated Regions; D000199:Actins; D003062:Codon; D002480:Cellulase