title: Proteomics for genetic and physiological studies in plants.
abstract: Proteomics is becoming a necessity in plant biology, as it is in medicine, zoology and microbiology, for deciphering the function and role of the genes that are or will be sequenced. In this review we focus on the various, mainly genetic, applications of the proteomic tools that have been developed in recent years: characterization of individuals or lines, estimation of genetic variability within and between populations, establishment of genetic distances that can be used in phylogenetic studies, characterization of mutants and localization of the genes encoding the revealed proteins. Improvements in specifically devoted software have permitted precise quantification of the variation in amounts of proteins, leading to the concept of "protein quantity loci" which, combined with the "quantitative trait loci" approach, results in testable hypotheses regarding the role of "candidate proteins" in the metabolism or phenotype under study. This new development is exemplified by the reaction of plants to drought, a trait of major agronomic interest. The accumulation of data regarding genomic and cDNA sequencing will be connected to the protein databases currently developed in plants.
authors.affiliation: Département de Botanique et Biologie Végétale, Université de Genève, Switzerland. herve.thiellement@bota.unige.ch
authors.forename: H
authors.initials: H
authors.lastname: Thiellement
authors.forename: N
authors.initials: N
authors.lastname: Bahrman
authors.forename: C
authors.initials: C
authors.lastname: Damerval
authors.forename: C
authors.initials: C
authors.lastname: Plomion
authors.forename: M
authors.initials: M
authors.lastname: Rossignol
authors.forename: V
authors.initials: V
authors.lastname: Santoni
authors.forename: D
authors.initials: D
authors.lastname: de Vienne
authors.forename: M
authors.initials: M
authors.lastname: Zivy
mesh_terms: D002874:Chromosome Mapping; D016208:Databases, Factual; D014644:Genetic Variation; D018745:Genome, Plant; D009154:Mutation; D010802:Phylogeny; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*
chemical_list: D010940:Plant Proteins