Effects of genistein on aggregation and cytosolic free calcium in pig platelets.
OBJECTIVE
To study the effects of genistein on aggregation and cytosolic free calcium concentration in platelets.


METHODS
Using turbidimetry to analyse aggregation and using Fura-2 fluorescence technique to determine Ca2+ level.


RESULTS
Genistein strongly inhibited the pig platelet aggregation induced by thrombin (250 U.L-1). When genistein concentrations were 5 and 20 mumol.L-1, the inhibition rates on the aggregation were 52% and 73%, respectively. Genistein inhibited the rise of cytosolic free calcium concentration in platelets stimulated by thrombin (500 U.L-1) in the presence of extracellular Ca2+ 1 mmol.L-1. When genistein concentrations were 10, 20, 40, and 80 mumol.L-1, the inhibition rates were 24%, 40%, 63%, and 65%, respectively, but no effect on thrombin-induced internal Ca2+ release from dense tubular system.


CONCLUSIONS
Genistein is a potential anti-platelet agent, mainly due to an inhibition of Ca2+ influx.
Liu
W
W
Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, China.
Song
Z J
ZJ
Liang
N C
NC
D000818:Animals; D001693:Biological Transport, Active; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D019833:Genistein / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013552:Swine
D010975:Platelet Aggregation Inhibitors; D019833:Genistein; D002118:Calcium