title: Molecular cloning and spatial expression of an ApL1 cDNA for the large subunit of ADP-glucose pyrophosphorylase from Arabidopsis thaliana.
abstract: A cDNA, ApL1a, corresponding to a homologue of the large subunit of ADP-glucose pyrophosphorylase (AGPase), has been isolated/characterised by screening a cDNA library prepared from leaves of Arabidopsis thaliana, followed by rapid amplification of cDNA 3'-ends (3'-RACE). Within the 1685 nucleotide-long sequence (excluding polyA tail), an open reading frame encodes a protein of 522 amino acids (aa), with a calculated molecular weight of 57.7 kDa. The derived aa sequence does not contain any discernible transit peptide cleavage site motif, similarly to two other recently sequenced full-length Arabidopsis homologues for AGPase, and shows ca. 58-78% identity to homologous proteins from other plants/tissues. The corresponding gene was found to be expressed in all tissues examined (rosette and stem leaves, stems, flowers and fruits). The ubiquitous expression of the gene is consistent with its critical role in starch synthesis in Arabidopsis.
authors.affiliation: Department of Plant Physiology, Umeå University, Sweden. leszek.kleczkowski@plantphys.umu.se
authors.forename: L A
authors.initials: LA
authors.lastname: Kleczkowski
authors.forename: L N
authors.initials: LN
authors.lastname: Sokolov
authors.forename: C
authors.initials: C
authors.lastname: Luo
authors.forename: P
authors.initials: P
authors.lastname: Villand
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D051599:Glucose-1-Phosphate Adenylyltransferase; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D018515:Plant Leaves; D018547:Plant Stems; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D009713:Nucleotidyltransferases; C496715:ADP-glucose pyrophosphorylase, Arabidopsis; D051599:Glucose-1-Phosphate Adenylyltransferase