title: Characterization and molecular cloning of a glutathione S-transferase gene from the tick, Boophilus microplus (Acari: Ixodidae).
abstract: A glutathione S-transferase (GST) was purified from the larval cattle tick, Boophilus microplus (Acari: Ixodidae), by glutathione-affinity chromatography. The purified enzyme appeared as a single band on SDS-PAGE and has a molecular mass of 25.8 kDa determined by mass spectrometry. The N-terminus of the purified enzyme was sequenced. The full-length cDNA of the enzyme was isolated by RT-PCR using degenerate oligonucleotides derived from the N-terminal amino acid sequence. The cDNA contains an open reading frame encoding a 223-amino-acid protein with the N-terminus identical to the purified GST. Comparison of the deduced amino acid sequence with GSTs from other species revealed that the enzyme is closely related to the mammalian mu class GST.
authors.affiliation: Food Animal Protection Research Laboratory, USDA-ARS, College Station, TX 77845, USA.
authors.forename: H
authors.initials: H
authors.lastname: He
authors.forename: A C
authors.initials: AC
authors.lastname: Chen
authors.forename: R B
authors.initials: RB
authors.lastname: Davey
authors.forename: G W
authors.initials: GW
authors.lastname: Ivie
authors.forename: J E
authors.initials: JE
authors.lastname: George
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005982:Glutathione Transferase / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007814:Larva; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D005982:Glutathione Transferase