title: Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission.
abstract: A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)g7 haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established.
authors.affiliation: First Department of Internal Medicine, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. nagafuchi@shs.kyushu-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Nagafuchi
authors.forename: H
authors.initials: H
authors.lastname: Katsuta
authors.forename: K
authors.initials: K
authors.lastname: Kogawa
authors.forename: T
authors.initials: T
authors.lastname: Akashi
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Tsukiyama
authors.forename: D
authors.initials: D
authors.lastname: Kitamura
authors.forename: Y
authors.initials: Y
authors.lastname: Niho
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D017931:DNA Primers; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D013234:Stem Cells / Q000166:cytology*
chemical_list: D017931:DNA Primers