The use of supercritical fluid extraction for the determination of steroids in animal tissues.
A multi-analyte, multi-matrix method was developed for the routine determination of steroids in animal tissues (skin, meat and fat). After addition of internal standards and sample pre-treatment, the analytes of interest were extracted from the matrix with unmodified supercritical CO2 and trapped directly on an alumina sorbent placed in the extraction vessel (in-line trapping under supercritical conditions). After extraction, alkaline hydrolysis was performed and the analytes were derivatised. The samples were then analysed by gas chromatography-mass spectrometry. The limit of detection for the different matrix-analyte combinations was 2 micrograms kg-1 (for melengestrol acetate 5 micrograms kg-1), the repeatability ranged from 4 to 42% (n = 9) and the reproducibility ranged from 2 to 39% (n = 3).
Stolker
A A
AA
National Institute of Public Health and the Environment, Laboratory for Residue Analysis, Bilthoven, The Netherlands.
Zoontjes
P W
PW
van Ginkel
L A
LA
D045930:Anabolic Agents / Q000032:analysis*; D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008460:Meat / Q000032:analysis*
D045930:Anabolic Agents