title: Simultaneous determination of serum retinol and alpha- and gamma-tocopherol levels in type II diabetic patients using high-performance liquid chromatography with fluorescence detection.
abstract: This paper presents a simple reversed-phase high-performance liquid chromatographic method for the simultaneous determination of retinol, and alpha- and gamma-tocopherols in human serum using a fluorescence detector. For chromatographic separation a binary gradient was used: phase A; acetonitrile-butanol (95:5); phase B; water, at a flow-rate of 1.5 ml/min. Serum retinol, and alpha- and gamma-tocopherol levels were measured in patients with non-insulin-dependent diabetes mellitus. Small sample requirement, good reproducibility and sensitivity make this method useful for the determination of the serum levels of these compounds in patients with diabetes mellitus.
authors.affiliation: Department of Chemistry and Biochemistry, Silesian Medical Academy, Katowice, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sobczak
authors.forename: B
authors.initials: B
authors.lastname: Skop
authors.forename: B
authors.initials: B
authors.lastname: Kula
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
chemical_list: D014801:Vitamin A; D014810:Vitamin E