Intrastriatal transplantation of Sertoli cells may improve amphetamine-induced rotation and tyrosine hydroxylase immunoreactivity of the striatum in hemiparkinsonian rats.
This study investigated survival and neurotrophic effects of Sertoli cells transplanted in the striatum of 6-hydroxydopamine (6-OHDA)-induced hemiparkinsonian rats. Primary cultures of Sertoli cells were established from 3-week old rats and characterized by associated marker, placental cadherin (P-cadherin). Two months after transplantation, amphetamine-induced rotations of rats transplanted with Sertoli cells were significantly lower than those of control rats. However, restoration of tyrosine hydroxylase (TH) immunoreactivity and Sertoli cells that expressed P-cadherin were only found in the striatum of the rat that showed full recovery from amphetamine-induced rotation 3 months after transplantation without immunosuppression. These results suggest that Sertoli cells transplanted in striatum of hemiparkinsonian rats may survive for at least 3 months, and improve amphetamine-induced rotation and restore TH immunoreactivity.
Liu
H W
HW
Department of Biology and Anatomy, National Defense Medical Center, Taipei 100, Taiwan. hwanliu@ndmc1.mdmctsgh.edu.tw
Kuang
Y J
YJ
Wu
J C
JC
Ma
K H
KH
Wang
S D
SD
Liu
J C
JC
D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000601:surgery*; D007150:Immunohistochemistry; D008297:Male; D010302:Parkinson Disease, Secondary / Q000201:enzymology / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D012708:Sertoli Cells / Q000637:transplantation*; D013552:Swine; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D000661:Amphetamine; D014446:Tyrosine 3-Monooxygenase