Meningiomas, dicentric chromosomes, gliomas, and telomerase activity.
Lack of telomere maintenance during cell replication leads to telomere erosion and loss of function. This can result in telomere associations which probably cause the dicentric chromosomes seen in some tumour cells. One mechanism of telomere maintenance in dividing cells is the action of telomerase, a ribonucleoprotein enzyme that adds TTAGGG repeats onto telomeres and compensates for their shortening during cell division. Over 90 per cent of extracranial malignant neoplasms have been found to have telomerase activity. This study sought to determine if there was a relationship between absence of telomerase activity and presence of dicentric chromosomes in meningiomas and to what extent the other main group of central nervous system tumours, the gliomas, expressed telomerase activity. Telomerase activity was measured on 25 meningiomas and 29 gliomas. Four of the meningiomas were atypical variants and 11 were positive for dicentric chromosomes. Twenty-five of 29 gliomas were glioblastoma multiforme tumours. Measures were taken to ensure absence of false positives due to primer-dimer interaction and false negatives due to protein degradation or the presence of Taq polymerase inhibitors. All 25 meningiomas and the four low-grade gliomas (WHO grade II) were telomerase activity-negative. Seven (28 per cent) of the 25 glioblastoma multiforme tumours showed telomerase activity. The absence of telomerase activity in meningiomas and the high frequency of telomere associations support the hypothesis that these tumours are benign, transformed but pre-crisis. The relatively low frequency of telomerase activity in the malignant glioblastoma multiforme suggests that most of these tumours may have other mechanisms of telomere maintenance and that the potentially therapeutic telomerase inhibitors will not be of great value in the future management of the majority of patients suffering from these tumours.
Carroll
T
T
Department of Neurosurgery, Royal Hallamshire Hospital, Sheffield, U. K.
Maltby
E
E
Brock
I
I
Royds
J
J
Timperley
W
W
Jellinek
D
D
D001932:Brain Neoplasms / Q000201:enzymology / Q000235:genetics*; D002869:Chromosome Aberrations; D005909:Glioblastoma / Q000201:enzymology / Q000235:genetics; D005910:Glioma / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008577:Meningeal Neoplasms / Q000201:enzymology / Q000235:genetics*; D008579:Meningioma / Q000201:enzymology / Q000235:genetics*; D009363:Neoplasm Proteins / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics
D009363:Neoplasm Proteins; D019098:Telomerase