Procoagulant and anticoagulant activities of resident and inflammatory peritoneal cells.
OBJECTIVE
In the present study, the involvement of both procoagulants and anticoagulants from adherent peritoneal cells in an inflammatory model was investigated.


METHODS
Mice were injected with thioglycollate broth or lipopolysaccharide (LPS).


METHODS
Cells were harvested by peritoneal lavage. Adherent peritoneal cells were cultured further +/- LPS. Subsequently, their ability to activate either Factor X or prothrombin, and their ability to inactivate thrombin through a thrombin-degrading mast cell chymase, was assayed.


RESULTS
Inflammatory cells expressed reduced amounts of thrombin-inactivating activity as compared with control resident peritoneal cells. Both resident and inflammatory cells expressed potent prothrombinase activities. Further stimulation of the various cellular populations with LPS in vitro had very little effect on the prothrombinase and thrombin-inactivating activities, bud had a strong stimulatory effect on the Factor X-activating activities.
Pejler
G
G
Swedish University of Agricultural Sciences, Department of Veterinary Medical Chemistry, The Biomedical Center, Uppsala. Gunnar.Pejler@vmk.slu.se
D000818:Animals; D002478:Cells, Cultured; D053818:Chymases; D004195:Disease Models, Animal; D005170:Factor X / Q000378:metabolism*; D005260:Female; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D012697:Serine Endopeptidases / Q000378:metabolism*; D013864:Thioglycolates / Q000276:immunology; D013917:Thrombin / Q000037:antagonists & inhibitors*; D013925:Thromboplastin / Q000378:metabolism*
D008070:Lipopolysaccharides; D013864:Thioglycolates; D005170:Factor X; D013925:Thromboplastin; D012697:Serine Endopeptidases; D053818:Chymases; D013917:Thrombin