title: Osteogenic differentiation of cultured marrow stromal stem cells on surface of microporous hydroxyapatite based mica composite and macroporous synthetic hydroxyapatite.
abstract: In order to investigate the significance of hydroxyapatite based microporous composite (HA/mica composite) surfaces and a macroporous synthetic hydroxyapatite, rat marrow cell culture, which shows osteogenic differentiation, was carried out on six different culture substrata (two control culture dishes, two identical HA/mica composites, and two identical macroporous synthetic hydroxyapatites). A culture period of two weeks in the presence of beta-glycerophosphate (BGP), ascorbic acid, and dexamethasone resulted in abundant mineralized nodule formations that were positive for alkaline phosphatase (ALP) stain. The stain on the macroporous synthetic hydroxyapatite and the HA/mica composites were intense, the enzyme activity being about double that of control culture dishes. These data indicate that the synthetic macroporous hydroxyapatite surface and the HA/mica composite surface promotes osteoblastic differentiation.
authors.affiliation: School of Science and Engineering, Teikyo University, Utsunomiya, Japan. egon.nordstrom@abo.fi
authors.forename: E
authors.initials: E
authors.lastname: Nordström
authors.forename: H
authors.initials: H
authors.lastname: Ohgushi
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: A T
authors.initials: AT
authors.lastname: Yokobori
authors.forename: T
authors.initials: T
authors.lastname: Yokobori
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001854:Bone Marrow Cells / Q000201:enzymology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D003907:Dexamethasone / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D005938:Glucocorticoids / Q000737:chemistry; D005994:Glycerophosphates / Q000737:chemistry; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000502:physiology*; D010006:Osteoblasts / Q000201:enzymology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity; D051381:Rats; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000538:Aluminum Silicates; D004396:Coloring Agents; D003470:Culture Media; D005938:Glucocorticoids; D005994:Glycerophosphates; D003907:Dexamethasone; D017886:Durapatite; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; C011934:mica; C031463:beta-glycerophosphoric acid