title: Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization.
abstract: Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to investigate the phylogenetic composition of bacterioplankton communities in several freshwater and marine samples. An average of about 50% of the cells were detected by probes for the domains Bacteria and Archaea, and of these, about half could be identified at the subdomain level with a set of group-specific probes. Beta subclass proteobacteria constituted a dominant fraction in freshwater systems, accounting for 16% (range, 3 to 32%) of the cells, although they were essentially absent in the marine samples examined. Members of the Cytophaga-Flavobacterium cluster were the most abundant group detected in the marine systems, accounting for 18% (range, 2 to 72%) of the 4',6-diamidino-2-phenylindole (DAPI) counts, and they were also important in freshwater systems (7%, range 0 to 18%). Furthermore, members of the alpha and gamma subclasses of Proteobacteria as well as members of the Planctomycetales were detected in both freshwater and marine water in abundances <7%.
authors.affiliation: Max-Planck-Institut für Marine Mikrobiologie, Bremen, Germany.
authors.forename: F O
authors.initials: FO
authors.lastname: Glöckner
authors.forename: B M
authors.initials: BM
authors.lastname: Fuchs
authors.forename: R
authors.initials: R
authors.lastname: Amann
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D015169:Colony Count, Microbial; D003589:Cytophaga / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes / Q000235:genetics; D005417:Flavobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D010933:Plankton / Q000235:genetics* / Q000302:isolation & purification*; D012623:Seawater / Q000382:microbiology*
chemical_list: D015342:DNA Probes