Rapid, high-sensitivity imaging of radiolabeled gels with microchannel plate detectors.
A microchannel plate detector has been used to image tritium-labeled protein on one- and two-dimensional electrophoresis gels. The good spatial resolution (70 microns) and high sensitivity (6.0 dpm/mm2) of the imaging system allows detection of low levels (femto moles) of labelled proteins. We are currently using the detector for identification of new targets involved in organophosphate neurotoxicity.
Lees
J E
JE
Space Research Centre, University of Leicester, UK. lee@star.le.ac.uk
Richards
P G
PG
D000818:Animals; D001923:Brain Chemistry; D004586:Electrophoresis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007525:Isoelectric Focusing; D007531:Isoflurophate / Q000633:toxicity; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009422:Nervous System Diseases / Q000139:chemically induced; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D051381:Rats; D012680:Sensitivity and Specificity; D014316:Tritium
D009419:Nerve Tissue Proteins; D009946:Organothiophosphorus Compounds; D014316:Tritium; D007531:Isoflurophate