Uniform band intensities in fluorescent dye terminator sequencing.
The use of Cyanine dye (Cy5 and Cy5.5) labeled dideoxy terminators with Thermo Sequenase DNA polymerase in DNA sequencing provides uniform band intensity, improved sequence read-length, and accuracy. It also greatly improves the ability to detect single base heterozygotes with dye-terminator sequencing method.
Kumar
S
S
Amersham Pharmacia Biotech, Cleveland, OH 44128, USA.
Fuller
C W
CW
Nampalli
S
S
Khot
M
M
Livshin
I
I
Sun
L
L
Hamilton
S
S
Samols
S B
SB
Mamone
J A
JA
Hujer
K M
KM
McArdle
B F
BF
Nelson
J R
JR
Duthie
S
S
D001483:Base Sequence; D004247:DNA; D005456:Fluorescent Dyes / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry
D005456:Fluorescent Dyes; D004247:DNA; D019914:Taq Polymerase