title: A peptide of the alpha3 chain of type IV collagen protects basement membrane against damage by PMN.
abstract: We have shown that basement membrane (BM) collagen (type IV), and specifically the peptide CNYYSNSYSFWLASLNPER (a.a. 185-203), from the non-collagenous domain of the alpha3 chain inhibits PMN. We examined the role of this peptide on PMN damage to BM in a vessel wall model. The presence of the endothelial monolayer as well as treatment of PMN with the alpha3(IV) 185-203 peptide reduced damage to BM by non-activated but not by activated PMN. The damage inhibition is unique to the alpha3(IV) peptide and not exhibited by comparable alpha1(IV) and alpha2(IV) chain peptides. A shorter peptide alpha3(IV) 185-191, containing the -SNS- triplet, reduced damage, whereas the one lacking the triplet, residues 194-203, was not effective. The CD47-alphavbeta3 integrin complex is the receptor for the alpha3(IV) peptide. Incubation of PMN with CD47 reactive mAb followed by the alpha3(IV) peptide abolished its protective effect on BM damage.
authors.affiliation: Connective Tissue Research Institute and Department of Medicine, University of Pennsylvania and University City Science Center, Philadelphia, Pennsylvania, 19104, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Ziaie
authors.forename: A
authors.initials: A
authors.lastname: Fawzi
authors.forename: G
authors.initials: G
authors.lastname: Bellon
authors.forename: J C
authors.initials: JC
authors.lastname: Monboisse
authors.forename: N A
authors.initials: NA
authors.lastname: Kefalides
mesh_terms: D000595:Amino Acid Sequence; D001485:Basement Membrane / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000209:etiology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D010446:Peptide Fragments; D003094:Collagen