Specialization and targeting of B-type cyclins.
The B-type cyclins of S. cerevisiae are diversified with respect to time of expression during the cell cycle as well as biological function. We replaced the early-expressed CLB5 coding sequence with the late-expressed CLB2 coding sequence, at the CLB5 locus. CLB5::CLB2 exhibited almost no rescue of clb5-specific replication defects, although it could rescue clb1 clb2 lethality, and in synchronized cells Clb2p-associated kinase activity from CLB5::CLB2 rose early in the cell cycle, similar to that of Clb5p. Mutagenesis of a potential substrate-targeting domain of CLB5 reduced biological activity without reducing Clb5p-associated kinase activity. Thus, Clb5p may have targeting domains required for CLB5-specific biological activity.
Cross
F R
FR
Rockefeller University New York, New York 10021, USA. fcross@rockvax.rockefeller.edu
Yuste-Rojas
M
M
Gray
S
S
Jacobson
M D
MD
D002453:Cell Cycle / Q000235:genetics; D019926:Cyclin B / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005804:Genes, Lethal; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D019926:Cyclin B; D005656:Fungal Proteins; D011494:Protein Kinases