Intraperitoneal transplantation of isologous mesothelial cells for prevention of adhesions.
OBJECTIVE
To study the effect of transplantation of mesothelial cells on the formation of adhesions after peritoneal abrasion.


METHODS
Animal study.


METHODS
Teaching hospital, Germany.


METHODS
30 isologous eight-week old Lewis rats to allow for harvesting of the greater omentum from a separate group.


METHODS
The first group (n = 10) served as mesothelial cell donors. The other animals had laparotomy and induction of adhesions by standardised abrasion of the peritoneum. The trial group (n = 10) were given a suspension of 10(6) mesothelial cells/100 g body weight intraperitoneally and the control group (n = 10) an equal volume of culture medium. After 10 days the animals were killed.


METHODS
Measurements of the areas of adhesions by computer aided morphometry.


RESULTS
The trial group developed a mean (SD) adhesion area of 122.7 (176.7) mm2, and the controls 310.5 (179.1) mm2. The corresponding medians (range) were 51.2 (0-547.1) and 274.3 (100.6-575.4). Transplantation of mesothelial cells resulted in a significant reduction in adhesion formation (Wilcoxon test, p<0.01).


CONCLUSIONS
Intraperitoneal transplantation of mesothelial cells is an effective way of reducing the formation of adhesions.
Bertram
P
P
Department of Surgery, Medical Faculty, Rhenish-Westphalian Technical University, Aachen, Germany.
Tietze
L
L
Hoopmann
M
M
Treutner
K H
KH
Mittermayer
C
C
Schumpelick
V
V
D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000637:transplantation*; D004848:Epithelium / Q000637:transplantation; D008297:Male; D009852:Omentum; D010532:Peritoneal Diseases / Q000517:prevention & control*; D010537:Peritoneum / Q000601:surgery*; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D000267:Tissue Adhesions / Q000517:prevention & control; D014185:Transplantation, Isogeneic; D014945:Wound Healing