title: Characterization of a canine long-term T cell line (DLC 01) established from a dog with Sézary syndrome and producing retroviral particles.
abstract: The canine DLC 01 cell line derives from a lymph node of a dog with Sézary syndrome. The DLC 01 cell phenotype is CD4-, CD8+, CD45+, DQ+, similar to that of original cells after treatment with dimethylsulfoxide or phorbol myristate. Canine cutaneous T cell lymphoma are usually CD4-, CD8+ in contrast to their human counterparts which are CD4+, CD8-. Therefore, the DLC 01 cell line appears to be a unique model to study the mechanism of all surface molecule expression in vitro. Viral particles with retrovirus type-C morphology were found in ultrathin sections of DLC 01 cell pellets. Retroviral particles are spontaneously produced after the 50th cell passage or after induction with 0.5% dimethylsulfoxide. This is the first description of a dog lymphoid cell line spontaneously growing and producing a retrovirus. It was found to share several features in common with feline and murine leukemia viruses.
authors.affiliation: Establissement de Transfusion Sanguine, Gerland, France.
authors.forename: I
authors.initials: I
authors.lastname: Ghernati
authors.forename: C
authors.initials: C
authors.lastname: Auger
authors.forename: L
authors.initials: L
authors.lastname: Chabanne
authors.forename: A
authors.initials: A
authors.lastname: Corbin
authors.forename: C
authors.initials: C
authors.lastname: Bonnefont
authors.forename: J P
authors.initials: JP
authors.lastname: Magnol
authors.forename: C
authors.initials: C
authors.lastname: Fournel
authors.forename: A
authors.initials: A
authors.lastname: Rivoire
authors.forename: J C
authors.initials: JC
authors.lastname: Monier
authors.forename: D
authors.initials: D
authors.lastname: Rigal
mesh_terms: D000818:Animals; D002415:Cats; D004283:Dog Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology; D004285:Dogs; D006801:Humans; D012190:Retroviridae / Q000302:isolation & purification; D012751:Sezary Syndrome / Q000276:immunology / Q000473:pathology / Q000662:veterinary / Q000821:virology; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology / Q000821:virology; D014407:Tumor Cells, Cultured