Application of high-performance liquid chromatography coupled to nuclear magnetic resonance spectrometry, mass spectrometry and bioassay for the determination of active saponins from Bacopa monniera Wettst.
Reversed-phase high-performance liquid chromatographic separation coupled to (structurally informative) spectroscopic methods like NMR and MS and an efficient bioassay have been used to determine the active compounds from a crude fraction of Bacopa monniera. The fraction containing a mixture of saponins with closely related structures was found to show a significant anthelmintic activity against Caenorhabditis elegans (used as a model test organism for determining anthelmintic activity). The activity was correlated to two dammarane type triterpenoidal saponins containing at least three sugar units. The optimization of separation for 1 mg of the crude sample on column and the sensitivity of on-flow one- and two-dimensional NMR experiments to the high-molecular-mass compounds (M(r) 890-930) has been demonstrated.
Renukappa
T
T
University of Hohenheim, Department of Chemistry, Stuttgart, Germany.
Roos
G
G
Klaiber
I
I
Vogler
B
B
Kraus
W
W
D000818:Animals; D000871:Anthelmintics / Q000032:analysis / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D017173:Caenorhabditis elegans / Q000187:drug effects; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000494:pharmacology
D000871:Anthelmintics; D012503:Saponins