Cloning, sequencing, and expression of cscA invertase from Escherichia coli B-62.
We have isolated a 2.5-kb DNA fragment from plasmid pST5R7 encoding a sucrose utilization system from Escherichia coli B-62 which confers a sucrose-fermenting phenotype to transformed E. coli K-12 strains. DNA-sequence determination revealed one full-length open reading frame 98% identical to cscA, the sucrose-hydrolase (invertase) gene of the csc regulon from E. coli EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsible for the sucrose-fermenting capacity of transformed E. coli K-12 strains carrying cscA.
Sahin-Tóth
M
M
Department of Physiology, University of California Los Angeles 90095-1662, USA. miklos@hhmi.ucla.edu
Lengyel
Z
Z
Tsunekawa
H
H
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D006026:Glycoside Hydrolases / Q000235:genetics*; D007785:Lactose / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D013997:Time Factors; D043324:beta-Fructofuranosidase
D001426:Bacterial Proteins; D003470:Culture Media; D013395:Sucrose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D007785:Lactose