title: Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998.
abstract: A polyphasic study of strains originally received as Bacillus (now Virgibacillus) pantothenticus, along with strains representing species belonging to Bacillus, Halobacillus and Paenibacillus, was undertaken using amplified rDNA restriction analysis (ARDRA), fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins and routine diagnostic characters comprising 61 biochemical tests in the API system and 15 observations of vegetative cell and sporangial morphology. It revealed the presence within Virgibacillus of an as yet undescribed new species, for which the name Virgibacillus proomii is proposed; V. proomii can be distinguished from V. pantothenticus and members of Bacillus sensu stricto, and from members of Paenibacillus and other aerobic endospore-forming bacteria, by routine phenotypic tests. The type strain of Virgibacillus proomii is LMG 12370T.
authors.affiliation: Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Heyndrickx
authors.forename: L
authors.initials: L
authors.lastname: Lebbe
authors.forename: K
authors.initials: K
authors.lastname: Kersters
authors.forename: B
authors.initials: B
authors.lastname: Hoste
authors.forename: R
authors.initials: R
authors.lastname: De Wachter
authors.forename: P
authors.initials: P
authors.lastname: De Vos
authors.forename: G
authors.initials: G
authors.lastname: Forsyth
authors.forename: N A
authors.initials: NA
authors.lastname: Logan
mesh_terms: D000818:Animals; D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000502:physiology; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S