Enantiomeric separations by capillary electrochromatography using a macrocyclic antibiotic chiral stationary phase.
Racemic mixtures of tryptophan and dinitrobenzoyl leucine have been successfully resolved by capillary electrochromatography (CEC) using the macrocyclic antibiotic teicoplanin, covalently bonded to a 5 microns silica support. Modification of a previously published packing procedure was required to pack reliable capillaries, capable of performing enantiomeric separations. Good levels of enantioselectivity were obtained in all cases, with optimised separations being performed in less than 6 min. Retention times, resolution and reproducibility are discussed.
Carter-Finch
A S
AS
Zeneca/SmithKline Beecham Centre for Analytical Sciences, Imperial College of Science, Technology and Medicine, London, UK.
Smith
N W
NW
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D007930:Leucine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D009579:Nitrobenzoates / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D017334:Teicoplanin / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D009579:Nitrobenzoates; D017334:Teicoplanin; C051872:N-(3,5-dinitrobenzoyl)leucine; D014364:Tryptophan; D007930:Leucine