title: Divergent effects of paracetamol on reactive oxygen intermediate and reactive nitrogen intermediate production by U937 cells.
abstract: We investigated the effects of paracetamol on reactive oxygen intermediate and reactive nitrogen intermediate production by U937 cells. Cells which had been treated with paracetamol (0.01 microM - 10 microM) exhibited a significant (p<0.001) increase in hydrogen peroxide production and a significant (p<0.001) increase in phorbol 12-myristate 13-acetate induced superoxide production. Conversely, paracetamol (0.01 microM - 10 microM) caused a significant (p<0.001) decrease in the amount of nitrite secreted into the culture medium. Our results indicate divergent effects of paracetamol on ROI and RNI production.
authors.affiliation: Division of Biomedical Sciences, School of Health Sciences, University of Wolverhampton, Wolverhampton WV1 1DJ, UK.
authors.forename: I
authors.initials: I
authors.lastname: Jamil
authors.forename: A
authors.initials: A
authors.lastname: Symonds
authors.forename: S
authors.initials: S
authors.lastname: Lynch
authors.forename: O
authors.initials: O
authors.lastname: Alalami
authors.forename: M
authors.initials: M
authors.lastname: Smyth
authors.forename: J
authors.initials: J
authors.lastname: Martin
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology*; D016227:Benzoquinones / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007097:Imines / Q000378:metabolism; D009000:Monocytes; D009573:Nitrites / Q000037:antagonists & inhibitors*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate; D020298:U937 Cells
chemical_list: D016227:Benzoquinones; D005609:Free Radicals; D007097:Imines; D009573:Nitrites; D017382:Reactive Oxygen Species; D013481:Superoxides; D000082:Acetaminophen; D006861:Hydrogen Peroxide; C028473:N-acetyl-4-benzoquinoneimine; D013755:Tetradecanoylphorbol Acetate