sst5 somatostatin receptor mRNA induction by mitogenic activation of human T-lymphocytes.
SRIF has neuro-immunomodulatory actions on immune cells, including T-lymphocytes. Molecular mechanisms involved in these actions were studied by RT-PCR analysis of SRIF receptor expression in resting and initogen-activated human T-lymphocytes. Our results point to the mitogen-associated induction of sst5 receptor subtype. Conversely, sst3 receptor appears constitutively expressed in both activity states. Assessment of biologic actions of SRIF14 in activated T-lymphocytes indicates that, in nanomolar concentration range, this peptide moderately inhibits mitogen-induced IL-2 secretion. Nevertheless, T-lymphocyte proliferation is not inhibited in the presence of SRIF14 but is even slightly increased. Altogether these data suggest a complex mechanism of SRIF neuro-immunomodulatory actions.
Ghamrawy
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Laboratoire de Biologie Cellulaire et Moléculaire, UMR 49, Ecole Normale Supérieure, Lyon, France.
Rabourdin-Combe
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C
Krantic
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D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D017481:Receptors, Somatostatin / Q000096:biosynthesis* / Q000276:immunology; D013004:Somatostatin / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D007376:Interleukin-2; D008934:Mitogens; D012333:RNA, Messenger; D017481:Receptors, Somatostatin; D013004:Somatostatin; C094573:somatostatin receptor 5