A nisin bioassay based on bioluminescence.
A Lactococcus lactis subsp. lactis strain that can sense the bacteriocin nisin and transduce the signal into bioluminescence was constructed. By using this strain, a bioassay based on bioluminescence was developed for quantification of nisin, for detection of nisin in milk, and for identification of nisin-producing strains. As little as 0.0125 ng of nisin per ml was detected within 3 h by this bioluminescence assay. This detection limit was lower than in previously described methods.
Wahlström
G
G
Institute of Biotechnology, FIN-00014 University of Helsinki, Finland.
Saris
P E
PE
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D001681:Biological Assay / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D008892:Milk / Q000737:chemistry; D009561:Nisin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D005520:Food Preservatives; D009561:Nisin