Determination of polychlorinated biphenyls in human blood serum by SPME.
We report a direct immersion SPME-GC/mu ECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9% to 3.4% (n = 10) was obtained. Analyte recovery up to 93% and detection limit of 1.0 ppb (total PCB) were achieved.
Poon
K F
KF
Department of Biology and Chemistry, City University of Hong Kong, Kowloon.
Lam
P K
PK
Lam
M H
MH
D000327:Adsorption; D001798:Blood Proteins / Q000378:metabolism; D020111:Chlorodiphenyl (54% Chlorine) / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D019286:Endopeptidase K; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000097:blood*; D015203:Reproducibility of Results
D001798:Blood Proteins; D010446:Peptide Fragments; D020111:Chlorodiphenyl (54% Chlorine); D011078:Polychlorinated Biphenyls; D019286:Endopeptidase K