[Influence of intraspinal implantation of pSVP0MCAT genetically modified Schwann cell in regeneration of injured spinal cord].
In order to observe the role of genetically modified Schwann cell (SC) with pSVP0Mcat in the regeneration of injured spinal cord, the cells were implanted into the spinal cord. Ninety SD rats were used to establish a model of hemi-transection of spinal cord at the level of T8, and were divided into three groups, randomly, that is, pSVP0Mcat modified SC implantation (Group A), SC implantation (Group B) and without cell implantation as control (Group C). After three months the presence of axonal regeneration of the injured spinal cord was examined by means of horseradish peroxidase (HRP) retrograde labelling technique and stereography. The results indicated that HRP labelled cells in Group A and B could be found in the superior region of injured spinal cord and the brain stem such as the red nuclei and oculomotor nuclei. The density of ventral hom neurons of the spinal cord and the number of myelinated axons in 100 microns of the white matter was A > B > C group. In brief, the pSVP0Mcat modified SC intraspinal implantation could promote regeneration of the injured spinal cord.
Chen
L
L
Department of Neurosurgery, First University Hospital, West China University of Medical Sciences, Chengdu, P.R. China.
Gao
L
L
Mao
B
B
D000818:Animals; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D008297:Male; D004676:Myelin Basic Protein / Q000235:genetics; D009416:Nerve Regeneration; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism / Q000637:transplantation*; D013116:Spinal Cord / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000601:surgery*
D004676:Myelin Basic Protein