An isolation procedure for arachidonic acid producing Mortierella species.
Malt extract agar and an incubation temperature of 5 degrees C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid.
Botha
A
A
Department of Microbiology and Biochemistry, University of the Orange Free State, South Africa. abo@maties.sun.ac.za
Paul
I
I
Roux
C
C
Kock
J L
JL
Coetzee
D J
DJ
Strauss
T
T
Maree
C
C
D016718:Arachidonic Acid / Q000096:biosynthesis*; D003470:Culture Media; D020094:Mortierella / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D009172:Mycology / Q000379:methods; D012988:Soil Microbiology; D013696:Temperature
D003470:Culture Media; D016718:Arachidonic Acid