Prevention of B220+ T cell expansion and prolongation of lifespan induced by Lactobacillus casei in MRL/lpr mice.
We examined the therapeutic effect of heat-killed Lactobacillus casei (LC) on MRL/lpr mice. Ingestion of a diet containing 0.05% (w/w) LC from the weaning period prolonged the lifespan and tended to reduce the proportion of B220+ T cells in the spleen and mesenteric lymph nodes (MLN) of MRL/lpr mice. When LC was intraperitoneally injected once a week after the age of 8 weeks, I-A- macrophages accumulated in the spleen as well as the peritoneum and macrophage progenitors increased in the bone marrow. Moreover, the amount of IL-6 mRNA in peritoneal macrophages was reduced by LC injection. Splenocytes from LC-injected MRL/lpr mice exhibited lower proliferative responses to mitogens than those from control MRL/lpr mice and the increase in number of B220+ T cells in the spleen and MLN was prevented by LC injection. However, LC injection affected neither expression of interferon-gamma (IFN-gamma) and IL-4 mRNAs nor proliferative capacities of splenic T cells. Our findings demonstrate that LC injection accelerates macrophage recruitment and prevents the expansion of B220+ T cells without affecting the functions of T cells in MRL/lpr mice. These immunological modulations induced by LC may lead to prolongation of the lifespan of MRL/lpr mice.
Mike
A
A
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Nagaoka
N
N
Tagami
Y
Y
Miyashita
M
M
Shimada
S
S
Uchida
K
K
Nanno
M
M
Ohwaki
M
M
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D002455:Cell Division / Q000276:immunology; D004032:Diet; D005260:Female; D007274:Injections, Intraperitoneal; D007780:Lacticaseibacillus casei / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008136:Longevity / Q000276:immunology*; D008206:Lymphatic Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D012965:Sodium Chloride / Q000008:administration & dosage; D013234:Stem Cells / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology
D012965:Sodium Chloride; D017493:Leukocyte Common Antigens