Fluorescent tissue site-selective lanthanide chelate, Tb-PCTMB for enhanced imaging of cancer.
In-vivo and in-vitro investigations indicate that a newly developed polyazamacrocyclic chelate of Tb(III) has superior properties for use as an abnormal tissue marker. In addition to tissue selectivity, this molecule is unique because of its low toxicity, attractive fluorescent properties, rapid pharmokinetics, and relatively high water solubility. The complex Tb-3,6,9-tris(methylene phosphonic acid n-butyl ester)-3,6,9,15-tetraazabicyclo[9.3.1]-pentadeca-1(15),11,13 -triene (Tb-PCTMB) has also been shown to exhibit strongly shifted emission (delta lambda--280 nm), moving the detection frequency away from autofluorescence backgrounds, and good quantum efficiencies (phi = 0.51), providing high brightness. Fluorescence imaging was used to quantify Tb-PCTMB at the picomolar level in tissues and to show the significant difference in affinity for the chelate by adenocarcinoma cells HT-29 versus normal epithelial cells (IEC-6). Topical application, or lavage introduction, under endoscopy was used to instill a millimolar aqueous solution of Tb-PCTMB into a dimethylhydrizene-treated Sprague Dawley rat large intestine containing a suspect growth. Subsequent in vitro fluorescence detection and standard histological evaluation confirmed enhanced uptake by adenocarcinoma tissue. Semiquantitative signal interrogation was employed to show the potential for using Tb-PCTMB as a contrast enhancement marker for disease detection.
Bornhop
D J
DJ
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061, USA. djbornhop@ttu.edu
Hubbard
D S
DS
Houlne
M P
MP
Adair
C
C
Kiefer
G E
GE
Pence
B C
BC
Morgan
D L
DL
D000818:Animals; D002470:Cell Survival; D002614:Chelating Agents; D006801:Humans; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D009942:Organometallic Compounds; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
D002614:Chelating Agents; D009942:Organometallic Compounds; C121445:Tb-PCTMB