Colony growth as a step towards axenization of Blastocystis isolates.
Colonies of Blastocystis from non-axenic cultures grown in agar medium were isolated from bacterial colonies. Axenization of human and reptilian isolates of Blastocystis was achieved using antibiotic treatment to lower bacterial numbers, followed by colony growth to isolate pure parasite colonies.
Ng
G C
GC
Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
Tan
K S
KS
D000818:Animals; D016844:Blastocystis / Q000254:growth & development* / Q000302:isolation & purification / Q000469:parasitology; D006801:Humans; D008116:Lizards