[ICAM-1 mRNA expression in the bronchial tissue of asthmatic guinea pigs].
To determine the expression of ICAM-1 mRNA in the bronchial tissue of asthmatic guinea pigs. The guinea pigs were divided randomly in to asthma, asthma treated with dexamethasone (DEX), and control groups. ICAM-1 expression was analyzed by in situ hybridization and the cellular infiltrate was evaluated by HE staining. In situ hybridization demonstrated ICAM-1 mRNA was mainly expressed in the cytoplasm of epithelial cells and endothelial cells. Compared with the control and DEX groups, a significant increase of ICAM-1 mRNA expression was observed in the bronchial tissue (P < 0.02 and 0.05 respectively). In asthmatic guinea-pigs, the intensity of ICAM-1 mRNA expression in bronchial tissue was correlated with total leucocyte infiltrate (P < 0.05). ICAM-1 expression in bronchial tissue may be related with the leucocyte infiltration and glucocorticoid may inhibit the expression of cell adhesion molecules.
Cao
G
G
Department of Respiratory Diseases, Daping Hospital, Third Military Medical University, Chongqing.
Yang
Z
Z
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001249:Asthma / Q000276:immunology*; D001980:Bronchi / Q000276:immunology*; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D017403:In Situ Hybridization; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation
D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone