PCR detection of Clostridium perfringens type D in formalin-fixed, paraffin-embedded tissues of goats and sheep.
A polymerase chain reaction (PCR) was used to identify the genes encoding the alpha, epsilon and beta toxins of Clostridium perfringens in formalin-fixed, paraffin-embedded intestinal tissues of goats and sheep. When pure cultures of Cl. perfringens types B and D were used as control templates in the PCR, products of the following sizes were observed on the agarose gel: 247 bp (alpha primers), 1025 bp (beta primers) and 403 bp (epsilon primers). When used to identify Cl. perfringens type D in formalin-fixed, paraffin-embedded intestinal tissues of goats and sheep, the PCR technique resulted in the detection of this micro-organism in 11 out of 13 samples known to be infected with Cl. perfringens. No false positive results were obtained when 13 culturally negative samples were analysed by the PCR technique.
Warren
A L
AL
Department of Microbiology, University of Queensland, Brisbane, Australia.
Uzal
F A
FA
Blackall
L L
LL
Kelly
W R
WR
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005557:Formaldehyde; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D007422:Intestines / Q000382:microbiology*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000382:microbiology; D013048:Specimen Handling; D016707:Tissue Fixation; D010738:Type C Phospholipases / Q000235:genetics
D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin; D005557:Formaldehyde; D010738:Type C Phospholipases