No functional beta 3-adrenergic receptors expressed in rat skeletal muscle cells.
OBJECTIVE
To determine the functional role of beta 3-adrenoceptors (beta 3-AR) in rat skeletal muscle cells.


METHODS
Nonselective beta-AR agonist isoprenaline (isoproterenol, Iso), beta 3-AR agonist CGP12177A which is a beta 1-/beta 2-AR antagonist and selective beta 3-AR antagonist SR59230A on cAMP accumulation was studied in primary cultured rat skeletal muscle cells.


RESULTS
Iso stimulated cAMP accumulation in a concentration-dependent manner with EC50 of 1.51 nmol.L-1 and propranolol inhibited cAMP accumulation stimulated by Iso with KB of 3.47 nmol.L-1. CGP12177A had no effect on cAMP accumulation but inhibited cAMP production induced by Iso. SR59230A 10 nmol.L-1 did not inhibit cAMP production induced by Iso.


CONCLUSIONS
The functional beta 3-AR are not present or at least not coupled to adenylyl cyclase activity in skeletal muscle cells.
Gao
B B
BB
Institute of Vascular Medicine, Third Hospital, Beijing Medical University, China.
Han
Q D
QD
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D007545:Isoproterenol / Q000037:antagonists & inhibitors; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D011412:Propanolamines / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D022702:Receptors, Adrenergic, beta-3
C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; C033394:CGP 12177