title: Cortexillin I is required for development in Polysphondylium.
abstract: The actin binding proteins cortexillin I and II play a major role in Dictyostelium cytokinesis, in which they are found localized to the membranes of the cleavage furrow. Here we report on cortexillin I mutants isolated by gene trapping in Polysphondylium. The original mutation and reconstructed versions of the original, as well as cortexillin I deletions, are unable to form aggregation streams under starvation conditions. The fruiting bodies that do form when cells are grown on bacterial lawns lack the one- and two-dimensional symmetries so apparent in wild type. These two phenotypes and the proposed structural basis for them suggest that cortexillin I functions in chemotaxis and morphogenesis in addition to its role in cytokinesis.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, 08544, USA.
authors.forename: P
authors.initials: P
authors.lastname: Fey
authors.forename: E C
authors.initials: EC
authors.lastname: Cox
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002455:Cell Division / Q000502:physiology; D002633:Chemotaxis; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000502:physiology; D019281:Dimerization; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D009235:Myxomycetes / Q000254:growth & development*; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins; D017386:Sequence Homology, Amino Acid; D013844:Thiazoles; D053778:Thiazolidines
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D008840:Microfilament Proteins; D020033:Protein Isoforms; D015800:Protozoan Proteins; D013844:Thiazoles; D053778:Thiazolidines; C101109:ctxA protein, Dictyostelium discoideum; C101110:ctxB protein, Dictyostelium discoideum; C037067:latrunculin A