ET-1 gene expression of rat brain during ischemia and reperfusion and the protective effect of radix Salviae miltiorrhizae.
Endothelin-1 (ET-1) gene expression of rat brain during ischemia and reperfusion as well as the effect of Radix Salviae Miltiorrhizae (RSM) were studied with in situ hybridization. It was found that ET-1 gene expression of cerebral cortex and caudate-putamen was markedly increased both in 24 hours of ischemia and 24 hours of reperfusion groups (P < 0.01, P < 0.05). In RSM-treated rats, although the ET-1 gene expressions of ischemia and reperfusion sides were also increased as compared with contralateral cortex and caudate-putamen, they were significantly lower in RSM-treated rats than those of controls (P < 0.05, P < 0.01 respectively). The present study indicated that RSM can partly inhibit ET-1 gene expression of cerebral cortex and caudate-putamen during ischemia and reperfusion. This may be one of the protective mechanisms of RSM on cerebral ischemia and reperfusion.
Wu
W
W
Department of Neurology, Chinese PLA General Hospital, Postgraduate Military Medical School, Beijing.
Kuang
P
P
Li
Z
Z
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D017403:In Situ Hybridization; D008297:Male; D010936:Plant Extracts; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D010936:Plant Extracts