Identification and localisation of ATP P2X receptors in rat midbrain.
P2X receptors that are gated by extracellular ATP are among the few known examples of ligand-gated cation-selective channels. There have been seven cloned proteins identified to date as members of the P2X receptor family in a wide range of tissues from the peripheral and central nervous systems and from many species. To determine the distribution of the P2X subtypes in the rat midbrain, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/Western blotting was combined with immunolocalisation using confocal microscopy. Subtypes P2X1-6 were detected in the periaqueductal gray area and the ependymal layer bordering the ventricle with a widespread distribution.
Worthington
R A
RA
Institute for Biomedical Research, and Department of Anatomy and Histology, The University of Sydney, NSW, Australia.
Arumugam
T V
TV
Hansen
M A
MA
Balcar
V J
VJ
Barden
J A
JA
D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000737:chemistry*; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D014018:Tissue Distribution
D018048:Receptors, Purinergic P2