Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera.
The immunogenic components of Fasciola gigantica excretory-secretory (ES) products were revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technic using sera from patients with F. gigantica infection, from patients with clinical suspected fascioliasis, from patients with other illness and from healthy adults. By SDS-PAGE, it was found that the ES products comprised more than 6 polypeptides. Immunoblotting analysis revealed 12 components which were strongly recognized by fascioliasis antisera. These antigenic components had a molecular mass ranging from less than 14.4 to 38 kDa. One antigenic band of 27 kDa was found to give a consistent reaction with fascioliasis antisera (100% sensitivity and 98% specificity). The present findings suggest that the 27 kDa components are sensitive and specific for the diagnosis of human F. gigantica infection.
Intapan
P M
PM
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand. wancham@medlib2.kku.ac.th
Maleewong
W
W
Wongkham
C
C
Tomanakarn
K
K
Ieamviteevanich
K
K
Pipitgool
V
V
Sukolapong
V
V
D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005209:Fasciola / Q000145:classification / Q000276:immunology*; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000947:Antigens, Helminth