SsrA-mediated peptide tagging caused by rare codons and tRNA scarcity.
SsrA RNA mediates the addition of a C-terminal peptide tag (AANDENYALAA) to bacterial proteins translated from mRNAs without in-frame stop codons. This process involves both tRNA- and mRNA-like functions of SsrA and targets the tagged proteins for degradation. By designing an SsrA variant that adds a peptide tag (AANDENYALDD) that does not result in rapid degradation, we show that tagging of a model protein synthesized from an mRNA without stop codons can be detected both in vivo and in vitro. We also use this assay to demonstrate that ribosome stalling at clusters of rare arginine codons in mRNA is sufficient to recruit and activate the SsrA peptide tagging system. An essential requirement for tagging at rare AGA codons is a scarcity of the cognate tRNA; supplemental tRNA(AGA) suppresses tagging, and depleting the available pool of tRNA(AGA) enhances tagging and reveals tagging caused by single rare AGA codons. Protein tagging at sites corresponding to rare codons appears to involve SsrA action at an internal mRNA site rather than at the 3' end of a cleaved mRNA.
Roche
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ED
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Sauer
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RT
D000595:Amino Acid Sequence; D003062:Codon; D004926:Escherichia coli; D008969:Molecular Sequence Data; D010455:Peptides; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger; D012343:RNA, Transfer
D003062:Codon; D010455:Peptides; D012329:RNA, Bacterial; D012333:RNA, Messenger; C096222:tmRNA; D012343:RNA, Transfer