Abnormal microtubule packing in processes of SF9 cells expressing the FTDP-17 V337M tau mutation.
Mutations in the gene for the microtubule associated protein, tau have been identified for fronto-temporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). In vitro data have shown that FTDP-17 mutant tau proteins have a reduced ability to bind microtubules and to promote microtubule assembly. Using the baculovirus system we have examined the effect of the V337M mutation on the organization of the microtubules at the ultrastructural level. Our results show that the organization of the microtubules is disrupted in the presence of V337M tau with greater distances between the microtubules and fewer microtubules per process.
Frappier
T
T
Department of Pathology and Taub Center for Alzheimer's Disease Research, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Liang
N S
NS
Brown
K
K
Leung
C L
CL
Lynch
T
T
Liem
R K
RK
Shelanski
M L
ML
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D003704:Dementia / Q000150:complications / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009154:Mutation; D010302:Parkinson Disease, Secondary / Q000150:complications / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera; D016875:tau Proteins / Q000235:genetics* / Q000378:metabolism*
D017931:DNA Primers; D011994:Recombinant Proteins; D016875:tau Proteins