Influence of epidermal growth factor (EGF) on renal transport of PAH and amino acids in amino acid loaded rats.
In anaesthetized adult female rats, the influence of epidermal growth factor (EGF) on renal transport of p-amino-hippurate (PAH), electrolytes, and amino acids was investigated. After loading with PAH (200 mg/100 g b.wt. iv.), PAH excretion in EGF treated rats (8 microg/100 g b.wt. subcutaneously for 8 days, twice daily 8 a.m. and 4 p.m.) was increased by about 20 %. Continuous infusions of glutamine, arginine (both 50 mg/100 g b.wt. per hour), or alanine (90 mg/ 100 g b.wt. per hour) were followed by an increase in the fractional excretion (FE) of the administered amino acids as well as of the other endogenous amino acids. Under load conditions (alanine, arginine or glutamine), EGF pretreatment was followed by a stimulation of renal amino acid reabsorption. These changes in amino acid transport were connected with a significant reduction of GFR after EGF pre-treatment (0.96+/-0.10 vs. 0.62+/-0.07 ml/min x 100 g b.wt.), with a distinct increase in sodium excretion (2.98+/-0.55 vs. 4.97+/-0.71 microval/100 g b.wt. x 20 min) and with a retarded normal kidney weight gain (874+/-18 vs. 775+/-32 mg/100 g b.wt.). A simultaneous PAH load reduced amino acid reabsorption as a sign of overloading of renal tubular transport capacity, but in EGF pretreated animals the amino acid excretion was only slightly increased under these conditions.
Fleck
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Institute of Pharmacology and Toxicology, Friedrich Schiller University of Jena, Germany. CFLE@mti-n.uni-jena.de
Pertsch
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D000042:Absorption; D000409:Alanine / Q000008:administration & dosage / Q000652:urine; D000596:Amino Acids / Q000008:administration & dosage* / Q000652:urine*; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000652:urine; D001692:Biological Transport / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005919:Glomerular Filtration Rate; D005973:Glutamine / Q000008:administration & dosage / Q000652:urine; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D010130:p-Aminohippuric Acid / Q000652:urine*
D000596:Amino Acids; D005973:Glutamine; D004815:Epidermal Growth Factor; D001120:Arginine; D000409:Alanine; D010130:p-Aminohippuric Acid