Glycodelin mRNA is expressed in the genital tract of male and female rats (Rattus norvegicus).
In the present study we demonstrate for the first time the expression of glycodelin mRNA in the female and male genital tracts of rats using non-radioactive in situ hybridisation. Glycodelin fragment 1 (+41 to +141) shares 100% homology with the human gene sequence. In the ovary, glycodelin mRNA was restricted to granulosa cells. In the uterus, glycodelin mRNA was expressed in all epithelial cells of the endometrium. In the male reproductive tract, glycodelin mRNA was distributed in all epithelial cells of the epididymis, the prostate and the seminal vesicle. However, in the testis, glycodelin mRNA was predominantly found in spermatogonia and in spermatocytes of the seminiferous epithelium. The expression in several reproductive organs of rats offers an excellent tool to study further the physiological role of glycodelin, which is so far thought to act as an immunosuppressive factor.
Keil
C
C
Institute of Biochemistry, Ernst Moritz Arndt University, Klinikum, Sauerbruchstrasse, D-17487 Greifswald, Germany.
Husen
B
B
Giebel
J
J
Rune
G
G
Walther
R
R
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D015870:Gene Expression; D005836:Genitalia, Female / Q000378:metabolism*; D005837:Genitalia, Male / Q000378:metabolism*; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
C489046:Gd protein, rat; D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D012313:RNA