Detection of Treponema socranskii associated with human periodontitis by PCR.
A PCR technique was used to detect and identify Treponema socranskii associated with periodontitis. A species-specific forward primer was designed for a variable region within its 16S rRNA gene and was used in conjunction with a conserved reverse primer. This primer pair was tested for specificity against 44 oral bacterial strains. Sensitivity was determined using a serial dilution of T. socranskii cells. Amplification products were obtained from all T. socranskii strains tested, but not from other oral bacteria associated with periodontal disease. The detection limit of PCR was 5 T. socranskii cells per PCR. T. socranskii was detected by PCR in subgingival plaque and saliva samples from patients with periodontitis.
Sakamoto
M
M
Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama. sakamoto@ulmus.riken.go.jp
Takeuchi
Y
Y
Umeda
M
M
Ishikawa
I
I
Benno
Y
Y
Nakase
T
T
D000328:Adult; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D003773:Dental Plaque / Q000382:microbiology; D005881:Gingiva / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014208:Treponema / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S