Securinine induced apoptosis in human leukemia HL-60 cells.
OBJECTIVE
To study whether securinine might induce apoptosis in human leukemia HL-60 cells.


METHODS
Inhibition of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. DNA fragmentation was visualized by DNA agarose gel electrophoresis and the cellular changes were observed by electron microscope.


RESULTS
Securinine 5-80 mg.L-1 elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in HL-60 cells. Securinine inhibited HL-60 cell proliferation in a time- and concentration-dependent manner. The IC50 and 95% confidence limits were 27 (15-47) mg.L-1 after 12-h treatment with securinine.


CONCLUSIONS
Securinine induced apoptosis in HL-60 cells.
Dong
N Z
NZ
Department of Pharmacology, Suzhou Medical College, China.
Gu
Z L
ZL
Chou
W H
WH
Kwok
C Y
CY
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001381:Azepines; D002455:Cell Division / Q000187:drug effects; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells / Q000648:ultrastructure; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D006801:Humans; D007783:Lactones; D010880:Piperidines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D001381:Azepines; D004273:DNA, Neoplasm; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D007783:Lactones; D010880:Piperidines; C000785:securinine