GATA3 is downregulated during hair cell differentiation in the mouse cochlea.
GATA3 is a transcription factor expressed in the inner ear during the early stages of development. A monoclonal antibody revealed that it is expressed in spiral ganglion cells and in all cells of the developing auditory sensory epithelium in the mouse before the hair cells differentiate at embryonic days 14-16. Expression decreases selectively in the hair cells as they differentiate progressively from the base to the apex of the developing organ of Corti. GATA3 subsequently decreases in the supporting cells and cannot be detected by immunofluorescence in any cell of the adult sensory epithelium. It is not expressed in the vestibular sensory epithelia or surrounding tissues from embryonic day 14. We suggest that GATA3 could act as a repressor of critical genes involved in cell differentiation in the organ of Corti, enabling a progressive formation of the adult cellular pattern.
Rivolta
M N
MN
Department of Physiology, School of Medical Sciences, University of Bristol, UK.
Holley
M C
MC
D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D003051:Cochlea / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D050990:GATA3 Transcription Factor; D005865:Gestational Age; D006198:Hair Cells, Auditory / Q000378:metabolism* / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D013136:Spiral Ganglion / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D015534:Trans-Activators