[Localization of NO-synthase in Lugaro cells and mechanisms of NO-ergic interactions between inhibitory interneurons of rabbit cerebellar cortex].
Using histochemical demonstration of NADPH-diaphorase (NADPH-d), several types of NO-producing cells were revealed in rabbit cerebellar cortex. Stellate and basket neurons of molecular layer, Golgi complex and white matter neurons are NADPH positive, while Purkinje cells are negative to NADPH-d. NADPH-d-positive granule cells are found in paraflocculus and flocculus, in vermis and paramedian lobules. They contain predominantly low enzyme activity or are not stained at all. In all the cerebellar cortex areas fusiform Lugaro cells lying under Purkinje cell bodies are stained intensely. Their horizontal dendrites are oriented parallel to Purkinje cells layer. Lugaro cells forming local axonal plexus in lower third of molecular layer and large Lugaro cells with the axon descending to granular layer and basket cell axons form contacts with Lugaro cell dendrites. Clusters of NADPH-d diaphorase positive cells were demonstrated. Dendrosomatic and somatosomatic tangential contacts of Lugaro cells between each other and dendro-dendritic contacts between Lugaro cells and Golgi cells were detected. Total number of Lugaro cells containing NADPH-d in vermis, paramedian lobules, right and left paraflocculus and flocculus equals to 5171. Absence of NO-ergic Lugaro cells in other mammals allows to conclude on the exceptional role of these neurons in rabbit cerebellar cortex.
Okhotin
V E
VE
Laboratory of Genetics and Developmental Neurogenetics, Russian Academy of Sciences, Moscow.
Kalinichenko
S G
SG
D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000201:enzymology*; D006651:Histocytochemistry; D007395:Interneurons / Q000201:enzymology*; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000032:analysis*; D011689:Purkinje Cells / Q000201:enzymology; D011817:Rabbits
D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase
Lokalizatsiia NO-sintazy v kletkakh Liugaro kletkakh i mekhanizmy NO-ergicheskogo vzaimodeĭstviia mezhdu tormoznymi interneĭronami kory mozzhechka krolika.