Random amplified polymorphic DNA analysis of Indian scented basmati rice (Oryza sativa L.) germplasm for identification of variability and duplicate accessions, if any.
Genetic variation among the seventeen basmati rice accessions collected from the farmers' fields located at different places in India was analyzed using ten arbitrary tenmer oligonucleotide primers by the random amplified polymorphic DNA (RAPD) method. The number of polymorphic/monomorphic bands among the pairwise combinations of the accessions and the total number of bands were determined to categorize all the accessions in different groups of similarity and dissimilarity at the genetic level. The procedure described also allows the identification and discrimination of the individual genotypes of basmati rice including the identification of the duplicates in genetic resource collections.
Verma
S K
SK
Department of Molecular Biology and Genetic Engineering, GB Plant University of Agriculture and Technology, Pantnagar, India. sunilverma@mailcity.com
Khanna
V
V
Singh
N
N
D018744:DNA, Plant / Q000235:genetics*; D014644:Genetic Variation; D012275:Oryza / Q000235:genetics*; D011110:Polymorphism, Genetic
D018744:DNA, Plant