Bone morphogenetic protein 1 regulates dorsal-ventral patterning in early Xenopus embryos by degrading chordin, a BMP4 antagonist.
Bone morphogenetic protein 1 (BMP1) is a metalloprotease that ventralises dorsal mesoderm when overexpressed in early Xenopus embryos. Here we show that Xenopus BMP1 blocks the dorsalising activity of chordin, but not noggin or DeltaxBMPR, when coexpressed in the ventral marginal zone and degrades chordin protein in vitro. We also show that a dominant-negative mutation for XBMP1 (dnBMP1) dorsalises ventral mesoderm in vivo, and blocks degradation of chordin by both XBMP1 and Xolloid, a closely related Xenopus metalloprotease, in vitro. dnBMP1 does not dorsalise ventral mesoderm in UV-irradiated embryos, demonstrating that this activity is dependent upon a functional organiser--the natural source of chordin in Xenopus gastrulae. Our results suggest that XBMP1 may regulate the availability of chordin during vertebrate embryogenesis.
Wardle
F C
FC
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Welch
J V
JV
Dale
L
L
D000818:Animals; D019521:Body Patterning / Q000502:physiology; D055395:Bone Morphogenetic Protein 1; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002352:Carrier Proteins; D004475:Ectoderm / Q000528:radiation effects; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000528:radiation effects; D018507:Gene Expression Regulation, Developmental; D005799:Genes, Dominant; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D008648:Mesoderm / Q000528:radiation effects; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology*; D000099176:Noggin Protein
D055395:Bone Morphogenetic Protein 1; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D008666:Metalloendopeptidases; D011506:Proteins; D029867:Xenopus Proteins; D000099176:Noggin Protein; C525812:bmp4 protein, Xenopus; C054234:chordin; C109187:TLL2 protein, Xenopus