The functional intronless S-adenosylmethionine decarboxylase gene of the mouse (Amd-2) is linked to the ornithine decarboxylase gene (Odc) on chromosome 12 and is present in distantly related species of the genus Mus.
S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the biosynthesis of polyamines. We have previously identified a mouse AdoMetDC gene that exhibits the hallmarks of a retroposon; that is, it has no introns, is flanked by direct repeats, and has a poly(dA) tract at its 3'-end. This gene, termed Amd-2, is not a processed pseudogene; rather, it is transcribed in a variety of mouse tissues and encodes a functional enzyme. In the current report, we present the sequence of a 6.7-kb genomic segment of the Amd-2 locus. Several sequences of interest, including an intercisternal A particle (IAP) element, a transposon-related sequence, and several expressed sequence tags (ESTs), were found within or near Amd-2. We also show, through analysis of an interspecific backcross, that Amd-2 is located on Chr 12, tightly linked to the gene (Odc) that encodes ornithine decarboxylase, another key enzyme in polyamine synthesis. Finally, we show that Amd-2 is present among several divergent species of the genus Mus. Thus, the integration event that generated Amd-2 may have occurred early during Mus evolution.
Persson
K
K
Department of Cellular and Developmental Biology, Umeâ University, S-901 87 Umeâ, Sweden.
Heby
O
O
Berger
F G
FG
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics*; D000818:Animals; D005075:Biological Evolution; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D007438:Introns; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D009955:Ornithine Decarboxylase / Q000235:genetics*; D010802:Phylogeny; D013045:Species Specificity
D004247:DNA; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase