Conserved and hybrid meta-cleavage operons from PAH-degrading Burkholderia RP007.
We have compared the sequence and gene order of meta-cleavage pathway operons from alpha- and gamma-subgroups of the Proteobacteria with operons from Burkholderia sp. strain RP007 which belongs to the beta-subgroup of the Proteobacteria. Burkholderia RP007 was isolated for its ability to degrade phenanthrene and contains two meta-cleavage operons. One exhibits a comparable gene order to previously characterised gamma-subgroup Proteobacterial (Pseudomonas) meta operons, whilst the other has distinctive features present in both alpha- and gamma-subgroup Proteobacterial (Sphingomonas and Pseudomonas) meta operons. Gene sequence conservation, highlighted by examining the phylogeny of Proteobacterial catechol 2,3-dioxygenase sequences, reveals that sequences generally cluster in a manner which correlates with the taxonomic grouping of the Proteobacterial subgroup from which they originated.
Laurie
A D
AD
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
Lloyd-Jones
G
G
D000818:Animals; D019117:Burkholderia / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D050548:Catechol 1,2-Dioxygenase; D002396:Catechols / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D049308:Dioxygenases; D019143:Evolution, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D009281:Naphthalenes / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics; D010616:Phenanthrenes / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D002396:Catechols; D009281:Naphthalenes; D010616:Phenanthrenes; C031721:naphthalene; C031181:phenanthrene; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; C034221:catechol