[An investigation of the polymorphism of HLA class II alleles in the Han population in Hubei Province of China].
OBJECTIVE
To investigate the polymorphism of HLA class II alleles in the Han population in Hubei province.


METHODS
The alleles of DRB1*(n=168), DQB1*(n=160) and DPB1*(n=93) were typed by using the polymerase chain reaction/sequence specific primer(PCR/SSP) and polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) techniques.


RESULTS
39 HLA-DRB1, 15 DQB1 and 17 DPB1 alleles were found. Alleles with higher frequencies are DRB1*0901 (GF=14%), *1501(GF=11.3%), *0301(GF=7.1%), *0803(GF=4.8%); DQB1*0301(GF=18.8%), *0303(GF=18.4%), *0201(GF=10%), *0302(GF=8.4%); DPB1*0501(GF=31.2%), *0401(GF=15.1%), *0201(GF=14%), *0402(GF=11.8%), respectively. As compared with Caucasians, the Han people in Hubei province have higher gene frequencies of DRB1*0901, *1001, *0803; DQB1*0303, *0502 and DPB1*0501, *0202; and lower gene frequencies of DRB1*0301, *0401, *1301; DQB1*0201, *0603 and DPB1*0401, suggesting that Hubei Hans have their own layout of HLA class II allele frequencies.


CONCLUSIONS
These data may serve as normal reference values for the Han population in south China.
Gong
F
F
Department of Immunology, Tongji Medical University, Wuhan, 430030 P. R.China. myxjys@tjmu.edu.cn
Xiong
P
P
Yang
Z
Z
Xu
Y
Y
Jiang
X
X
Wu
X
X
Liu
M
M
Fong
W
W
Ferencik
S
S
Liu
J
J
Grosse-Wilde
H
H
D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length