Factors affecting epicardial dispersion of repolarization: a mapping study in the isolated porcine heart.
OBJECTIVE
Non-uniform drug-induced prolongation of repolarization predominating in the midmyocardial (M) cell layers has been shown to be responsible for perpetuation of reentry, giving rise to torsade de pointes. However, the absence of M cells in immature animals, especially the pig, suggests other possible underlying mechanisms. We sought to examine, in this species, the effects of predisposing factors to torsade de pointes on the dispersion of epicardial repolarization and their contribution to arrhythmogenesis.


METHODS
Computerized mapping of repolarization and activation was conducted on the epicardial surface in 29 Langendorff-perfused hearts of eight-week-old pigs. Activation-recovery intervals were measured simultaneously from 128 unipolar electrograms.


RESULTS
Baseline iso-interval maps were dipolar (41%) or multipolar (59%). Dispersion of repolarization was reverse frequency-dependent but was unaffected by lowering [K+]o. DL-Sotalol (0.1 mmol/l) reinforced local gradients and thus increased epicardial dispersion, whereas intramural recordings did not demonstrate any predominant effect in midmyocardial layers. Phenylephrine (1 mumol/l) notably augmented DL-sotalol effects. After [Mg++]o lowering, although dispersion was not significantly increased, DL-sotalol was associated with the spontaneous occurrence of polymorphic ventricular tachycardia in seven out of nine experiments. When maps of repolarization of escape beats were compared with activation maps of first arrhythmic beats, an arc of functional dissociation was observed in the vicinity of a steep gradient of repolarization in two out of nine tachycardias.


CONCLUSIONS
Epicardial dispersion of repolarization is increased by slow rates, DL-sotalol and phenylephrine but is not the only requirement for initiation of polymorphic ventricular tachycardia. In combination with other factors, it helps continuation of the arrhythmia in this model.
Lacroix
D
D
Department of Cardiology, University of Lille, France. dlacroix@chru-lille.fr
Extramiana
F
F
Delfaut
P
P
Adamantidis
M
M
Grandmougin
D
D
Klug
D
D
Kacet
S
S
Dupuis
B
B
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D004594:Electrophysiology; D005260:Female; D007091:Image Processing, Computer-Assisted; D010477:Perfusion; D010496:Pericardium / Q000187:drug effects / Q000503:physiopathology*; D010656:Phenylephrine / Q000494:pharmacology; D013015:Sotalol / Q000494:pharmacology; D013552:Swine; D016171:Torsades de Pointes / Q000503:physiopathology*
D000316:Adrenergic alpha-Agonists; D000319:Adrenergic beta-Antagonists; D010656:Phenylephrine; D013015:Sotalol