Quercetin down-regulated bcl-2 gene expression in human leukemia HL-60 cells.
OBJECTIVE
To study the effect of quercetin (Que) on bcl-2 gene expression in human leukemia HL-60 cells.


METHODS
Immunohistochemical analysis and RNA Dot blot hybridization were used to identify the expression of bcl-2 genes.


RESULTS
The expression of bcl-2 protein was 94% in control HL-60 cells, which became 45%-84% when they were cultured with Que 15-60 mumol.L-1 for 48 h. The expression of bcl-2 mRNA in HL-60 cells was obviously decreased in treatment with Que 15-60 mumol.L-1.


CONCLUSIONS
The apoptotic action of Que in HL-60 cells was associated with the down-regulation of the apoptosis-suppressing gene bcl-2.
Xiao
D
D
Department of Pharmacology, Suzhou Medical College, China.
Gu
Z L
ZL
Zhu
S P
SP
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019254:Genes, bcl-2; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D011794:Quercetin