title: Myosin-V is a processive actin-based motor.
abstract: Class-V myosins, one of 15 known classes of actin-based molecular motors, have been implicated in several forms of organelle transport, perhaps working with microtubule-based motors such as kinesin. Such movements may require a motor with mechanochemical properties distinct from those of myosin-II, which operates in large ensembles to drive high-speed motility as in muscle contraction. Based on its function and biochemistry, it has been suggested that myosin-V may be a processive motor like kinesin. Processivity means that the motor undergoes multiple catalytic cycles and coupled mechanical advances for each diffusional encounter with its track. This allows single motors to support movement of an organelle along its track. Here we provide direct evidence that myosin-V is indeed a processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament.
authors.affiliation: Department of Biochemistry, Stanford University Medical Center, California 94305, USA.
authors.forename: A D
authors.initials: AD
authors.lastname: Mehta
authors.forename: R S
authors.initials: RS
authors.lastname: Rock
authors.forename: M
authors.initials: M
authors.lastname: Rief
authors.forename: J A
authors.initials: JA
authors.lastname: Spudich
authors.forename: M S
authors.initials: MS
authors.lastname: Mooseker
authors.forename: R E
authors.initials: RE
authors.lastname: Cheney
mesh_terms: D000199:Actins / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000502:physiology*; D002384:Catalysis; D002645:Chickens; D066298:In Vitro Techniques; D020409:Molecular Motor Proteins / Q000502:physiology*; D009068:Movement; D018994:Myosin Light Chains / Q000502:physiology*; D024701:Myosin Type V; D009419:Nerve Tissue Proteins / Q000502:physiology*; D055095:Optics and Photonics
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins; D020409:Molecular Motor Proteins; D018994:Myosin Light Chains; D009419:Nerve Tissue Proteins; C078829:chicken brain myosin-V p190; D000255:Adenosine Triphosphate; D024701:Myosin Type V