title: In vitro isolation of Neospora caninum from a stillborn calf in the UK.
abstract: Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR) techniques.
authors.affiliation: Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, Pembroke Place, Liverpool, L3 5QA, UK.
authors.forename: H C
authors.initials: HC
authors.lastname: Davison
authors.forename: F
authors.initials: F
authors.lastname: Guy
authors.forename: A J
authors.initials: AJ
authors.lastname: Trees
authors.forename: C
authors.initials: C
authors.lastname: Ryce
authors.forename: J T
authors.initials: JT
authors.lastname: Ellis
authors.forename: A
authors.initials: A
authors.lastname: Otter
authors.forename: M
authors.initials: M
authors.lastname: Jeffrey
authors.forename: V R
authors.initials: VR
authors.lastname: Simpson
authors.forename: J J
authors.initials: JJ
authors.lastname: Holt
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002522:Chlorocebus aethiops; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D005313:Fetal Death / Q000469:parasitology* / Q000662:veterinary*; D018688:Neospora / Q000302:isolation & purification*; D019105:Random Amplified Polymorphic DNA Technique; D006113:United Kingdom; D014709:Vero Cells