Minocycline modulation of alpha-MSH production by keratinocytes in vitro.
The anti-inflammatory mechanisms of minocycline, an antibiotic used in the treatment of the inflammatory component of acne, are only partially understood. In addition to inflammation due to cytokines (IL-1, IL-6, TNF-alpha, etc.), recent studies have shown that neuropeptide-mediated neurogenic inflammation may play an important role in cutaneous inflammation. The purpose of this study was to investigate minocycline-induced modulation of cutaneous production of alpha-melanocyte-stimulating hormone (alpha-MSH), a neuropeptide with known anti-inflammatory activity. Two different skin models were used: explants of inflammatory skin and reconstituted skin, both incubated with minocycline at different concentrations and for different time periods. Epidermal production of alpha-MSH, as evaluated by immunofluorescence and immunoperoxidase techniques, showed increased expression in both models. This neuropeptide, which has an anti-inflammatory activity (notably through production of IL-10, antagonism of IL-1 and inhibition of the chemotaxis of polymorphonuclear leukocytes), thus plays a role in the anti-inflammatory action of minocycline.
Sainte-Marie
I
I
Department of Dermatology, Hôtel-Dieu Hospital, Nantes, France.
Tenaud
I
I
Jumbou
O
O
Dréno
B
B
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008911:Minocycline / Q000494:pharmacology*; D011565:Psoriasis / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D000521:alpha-MSH / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D000521:alpha-MSH; D008911:Minocycline