title: Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery.
abstract: Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemia-reperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3, 24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes (% dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin-eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.
authors.affiliation: Department of Radiology, Fujita Health University, Toyoake, Aichi, Japan. htoyama@fujita-hu.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Toyama
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Naito
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
authors.forename: K
authors.initials: K
authors.lastname: Kikukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Komori
authors.forename: A
authors.initials: A
authors.lastname: Hasumi
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Ejiri
authors.forename: K
authors.initials: K
authors.lastname: Senda
authors.forename: A
authors.initials: A
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Koga
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008099:Liver / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000201:enzymology / Q000473:pathology; D008115:Liver Regeneration; D008297:Male; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000032:analysis*; D015427:Reperfusion Injury / Q000000981:diagnostic imaging* / Q000201:enzymology / Q000473:pathology; D013668:Technetium Tc 99m Aggregated Albumin; D016284:Technetium Tc 99m Pentetate
chemical_list: D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D011956:Receptors, Cell Surface; D013668:Technetium Tc 99m Aggregated Albumin; C070561:technetium Tc 99m DTPA-galactosyl-human serum albumin; D016284:Technetium Tc 99m Pentetate