Inhibition and binding studies of coenzyme A and bovine phenol sulfotransferase.
Phenol sulfotransferases (PSTs, EC 2.8.2.1) catalyze sulfonyl group transfer from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to the hydroxyl oxygen of aromatic acceptor substrates. The structural overlap between PAPS and coenzyme A (CoA) suggested a possible role of this common acyl carrier in modulating PST activity. To test this hypothesis, purified recombinant bovine PST was examined by kinetic and affinity chromatographic approaches. After demonstrating PST enzyme inhibition by CoA, systematic variation of CoA and PAPS concentrations indicated simple competitive inhibition with K(i) = 1. 3 microM. PST bound to CoA-agarose, attached via the pantetheinyl thiol group, was eluted with PAP but not by 2-naphthol. This observation was consistent with the pattern of inhibition. Additional members of the sulfotransferase superfamily, as well as acylated CoAs, should be further investigated.
Leach
M
M
Department of Biochemistry, Alma College, 614 W. Superior Street, Alma, Michigan, 48801, USA.
Cameron
E
E
Fite
N
N
Stassinopoulos
J
J
Palmreuter
N
N
Beckmann
J D
JD
D000818:Animals; D015239:Arylsulfotransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D001667:Binding, Competitive; D002417:Cattle; D002846:Chromatography, Affinity; D003065:Coenzyme A / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D015394:Molecular Structure; D010724:Phosphoadenosine Phosphosulfate / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D010724:Phosphoadenosine Phosphosulfate; D015239:Arylsulfotransferase; D003065:Coenzyme A