title: A YAC-based physical map of the mouse genome.
abstract: A physical map of the mouse genome is an essential tool for both positional cloning and genomic sequencing in this key model system for biomedical research. Indeed, the construction of a mouse physical map with markers spaced at an average interval of 300 kb is one of the stated goals of the Human Genome Project. Here we report the results of a project at the Whitehead Institute/MIT Center for Genome Research to construct such a physical map of the mouse. We built the map by screening sequenced-tagged sites (STSs) against a large-insert yeast artificial chromosome (YAC) library and then integrating the STS-content information with a dense genetic map. The integrated map shows the location of 9,787 loci, providing landmarks with an average spacing of approximately 300 kb and affording YAC coverage of approximately 92% of the mouse genome. We also report the results of a project at the MRC UK Mouse Genome Centre targeted at chromosome X. The project produced a YAC-based map containing 619 loci (with 121 loci in common with the Whitehead map and 498 additional loci), providing especially dense coverage of this sex chromosome. The YAC-based physical map directly facilitates positional cloning of mouse mutations by providing ready access to most of the genome. More generally, use of this map in addition to a newly constructed radiation hybrid (RH) map provides a comprehensive framework for mouse genomic studies.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
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authors.lastname: Nusbaum
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authors.initials: DK
authors.lastname: Slonim
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authors.initials: KL
authors.lastname: Harris
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authors.initials: BW
authors.lastname: Birren
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authors.initials: LD
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authors.lastname: Devine
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authors.initials: LT
authors.lastname: Horton
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authors.initials: W
authors.lastname: Ye
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: V
authors.initials: V
authors.lastname: Kouyoumjian
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authors.initials: IS
authors.lastname: Zemsteva
authors.forename: Y
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authors.lastname: Wu
authors.forename: A J
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authors.lastname: Collymore
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authors.initials: DF
authors.lastname: Courtney
authors.forename: J
authors.initials: J
authors.lastname: Tam
authors.forename: M
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authors.lastname: Cadman
authors.forename: A R
authors.initials: AR
authors.lastname: Haynes
authors.forename: C
authors.initials: C
authors.lastname: Heuston
authors.forename: T
authors.initials: T
authors.lastname: Marsland
authors.forename: A
authors.initials: A
authors.lastname: Southwell
authors.forename: P
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authors.lastname: Trickett
authors.forename: M A
authors.initials: MA
authors.lastname: Strivens
authors.forename: M T
authors.initials: MT
authors.lastname: Ross
authors.forename: W
authors.initials: W
authors.lastname: Makalowski
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: M S
authors.initials: MS
authors.lastname: Boguski
authors.forename: N P
authors.initials: NP
authors.lastname: Carter
authors.forename: P
authors.initials: P
authors.lastname: Denny
authors.forename: S D
authors.initials: SD
authors.lastname: Brown
authors.forename: T J
authors.initials: TJ
authors.lastname: Hudson
authors.forename: E S
authors.initials: ES
authors.lastname: Lander
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D020451:Contig Mapping; D005819:Genetic Markers; D016678:Genome; D051379:Mice / Q000235:genetics*; D008957:Models, Genetic; D020161:Physical Chromosome Mapping
chemical_list: D005819:Genetic Markers