Development of a new DNA sequencing method: 3'-ester cleavage catalyzed by Taq DNA polymerase.
A new 3'-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.
Rasolonjatovo
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I
Unité de Chimie Organique, Institut Pasteur, Paris, France.
Sarfati
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SR
D002384:Catalysis; D004952:Esters; D006868:Hydrolysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism*
D004952:Esters; D019914:Taq Polymerase