title: Urease-positive thermophilic strains of Campylobacter isolated from seagulls (Larus spp.).
abstract: Three strains of urease-positive thermophilic Campylobacter (UPTC), designated A1, A2 and A3, were identified by biochemical characterization after isolation from faeces of seagulls in Northern Ireland in 1996. The biochemical characteristics of the strains were identical to those of strains described previously. Analysis by pulsed-field gel electrophoresis (PFGE) after separate digestion with ApaI and SmaI demonstrated that the respective PFGE profiles were indistinguishable. The PFGE analysis also suggested that the genomes were approximately 1810 kb in length. This is the first example of the isolation of UPTC from flying homoiothermal animals, i.e. from seagulls (Larus spp.).
authors.affiliation: Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Miyajima
authors.forename: J E
authors.initials: JE
authors.lastname: Moore
authors.forename: P G
authors.initials: PG
authors.lastname: Murphy
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001717:Birds / Q000382:microbiology*; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D009660:Northern Ireland; D013696:Temperature; D014510:Urease / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D014510:Urease