A metallic multisite recording system designed for continuous long-term monitoring of electrophysiological activity in slice cultures.
This paper describes a flexible, metallic multielectrode array, made on kapton to fit in a recording chamber for interface-type organotypic cultures. This multisite recording system is designed for continuous multisite monitoring of electrophysiological activity in rat brain organotypic slice cultures. The system is composed of a signal conditioning set-up, which also masters electrical stimulation paradigms and a card containing the microelectrode array. The card comprises a perfusion chamber closed by a rigid and permeable membrane on which the pierced microelectrode array supporting the slice culture is placed. Once closed with a gaseous chamber, the inside of the card remained sterile and free of contamination and could be maintained inside or outside the incubator for electrophysiological analyses. Dimensions of each 28-plated gold microelectrode recording site are 50 microns x 100 microns. The design of the chambers and the card makes it possible to modify both the perfusion medium and the gaseous atmosphere in sterile conditions, allowing thus analyses of long-term effects of pharmacological compounds. Using this array one can perform stimulation and recordings of the electrical activity of the slice. Signals obtained with this reusable system exhibit a good signal-to-noise ratio. This device was tested to follow the evolution and modifications of the evoked and/or spontaneous electrical activity of the same groups of neurones during several days.
Duport
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Division de Neuropharmacologie, CMU, Geneva 4, Switzerland.
Millerin
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C
Muller
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D
Corrèges
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P
D000818:Animals; D001921:Brain / Q000502:physiology*; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D008567:Membranes, Artificial; D008670:Metals; D008839:Microelectrodes; D008991:Monitoring, Physiologic / Q000379:methods*; D009924:Organ Culture Techniques; D016062:Porosity; D051381:Rats
D008567:Membranes, Artificial; D008670:Metals