Effects of active immunization with a pneumococcal surface protein (PspA) and of locally applied antibodies in experimental otitis media.
An experimental rat model for Streptococcus pneumoniae otitis media was used to investigate passive protection by anti-type 3 capsular antibodies and effects of immunization with pneumococcal surface protein A (PspA). Anti-type 3 antibodies instilled into the middle ear reduced purulent otitis media as compared to control animals (p = 0.015). Secondly, after immunization with PspA, the right middle ear was inoculated with S. pneumoniae type 6A in a dose calibrated to induce purulent otitis media. There was an anti-PspA antibody response in all rats immunized and a reduction in signs of purulent otitis media as compared to control animals (p = 0.026). Thus, purulent (acute) otitis media can be reduced by local application of antibodies in the middle ear and also by immunization with a non-type-specific pneumococcal protein, PspA.
White
P
P
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital, Lund, Sweden. Peter.White@onh.lu.se
Hermansson
A
A
Svanborg
C
C
Briles
D
D
Prellner
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D004195:Disease Models, Animal; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D010035:Otitis Media, Suppurative / Q000517:prevention & control*; D011008:Pneumococcal Infections / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013290:Streptococcal Infections / Q000517:prevention & control*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D014611:Vaccination
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins