Recombinant adenoviruses expressing the E2 protein of bovine viral diarrhea virus induce humoral and cellular immune responses.
The E2 protein of bovine viral diarrhea virus (BVDV) is a major viral glycoprotein and an attractive target for BVDV vaccines. Three replication defective recombinant adenoviruses expressing the BVDV/E2 protein (rAds/E2) were constructed. Two contain a constitutive promoter, and one an inducible promoter. All three recombinant adenoviruses induced very strong BVDV specific antibody responses in a mouse model as detected by enzyme-linked immunosorbant assay (ELISA) and neutralization tests. Induction of cellular immune responses was investigated in two recombinant adenoviruses with a constitutive promoter. The mononuclear cells from the immunized mice demonstrated a proliferative response after in vitro stimulation with an homologous BVDV strain, but only one of them induced the production of IFN-gamma.
Elahi
S M
SM
Virology Section, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Que., Canada.
Shen
S H
SH
Talbot
B G
BG
Massie
B
B
Harpin
S
S
Elazhary
Y
Y
D000256:Adenoviridae; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C076551:gp53, bovine viral diarrhea virus