Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis.
Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3, 6-tri-O-methyl)-beta-cyclodextrin(TM-beta-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-beta-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 nM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.
Tsunoda
M
M
Graduate School of Pharmaceutical Sciences, University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Kato
M
M
Fukushima
T
T
Santa
T
T
Homma
H
H
Yanai
H
H
Soga
T
T
Imai
K
K
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D019668:Liliaceae / Q000737:chemistry; D008297:Male; D010870:Pineal Gland / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism; D014920:Wine / Q000032:analysis
D003505:Cyclodextrins; D007202:Indicators and Reagents; D001224:Aspartic Acid