Lectin binding patterns in nonsensory regions of rat cochlea during postnatal development.
The distribution of glycoconjugates was examined in the nonsensory regions of the rat cochlea during postnatal development using biotin-conjugated lectins. Temporal bones of rats at postnatal d 1 and at wk 2, 4 and 6 were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde and processed for paraffin wax embedding. The dewaxed sections were incubated with 7 biotinylated lectins, followed by avidin-biotin-peroxidase complex. A different staining pattern was observed in the stria vascularis, spiral ligament and spiral limbus in the age groups examined. The staining intensity varied between lectins and the reaction product exhibited limited disparity. The staining intensity for WGA increased with age in all the 3 nonsensory regions. The staining patterns for the other lectins differed in the various nonsensory regions examined indicating tissue specificity. The limited variations in the lectin binding patterns after 2nd wk of postnatal life also indicate that the changes in the carbohydrate moieties are established during the fetal period of cochlear development and limited changes take place during postnatal maturation of the nonsensory regions.
Khan
K M
KM
Department of Anatomy, The Aga Khan University, Karachi, Pakistan. khalid.khan@aku.edu
Sarfaraz
N
N
Salim
Z
Z
D000818:Animals; D003051:Cochlea / Q000737:chemistry* / Q000254:growth & development*; D003208:Concanavalin A / Q000032:analysis; D005260:Female; D006001:Glycoconjugates / Q000032:analysis*; D006651:Histocytochemistry; D037102:Lectins / Q000032:analysis; D008297:Male; D019887:Peanut Agglutinin / Q000032:analysis; D037121:Plant Lectins; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins; D013316:Stria Vascularis / Q000737:chemistry; D013997:Time Factors; D014909:Wheat Germ Agglutinins / Q000032:analysis; D010835:Phytohemagglutinins
D003208:Concanavalin A; D006001:Glycoconjugates; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C017707:Ricinus communis agglutinin-1; C036850:Ulex europaeus lectins; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins