Chromosome-type aberrations induced in chromosome 9 after treatment of human peripheral blood lymphocytes with mitomycin C at the G(0) phase.
To determine the fate of chromosome aberrations induced primarily by clastogenic chemicals, aberrations of chromosome 9 in cultured human peripheral blood lymphocytes were analyzed after exposure to mitomycin C (MMC) at G(0) phase. Chromosome 9 painting by fluorescence in situ hybridization revealed that the translocation of 9p or 9q to another chromosome and the centric fragment representing the entire length of 9p were characteristically generated from chromatid-type aberrations involving the centromeric region of chromosome 9. These changes were not observed at 48 h after culture initiation, but persistently appeared at later stages (72-120 h postinitiation). Induction of centric fragments of 9p and micronuclei without the alpha satellite DNA of chromosome 9 suggested that most of the breaks were induced near the alpha satellite DNA locus on 9q. Modified patterns of chromosome 9 aberrations were also observed, being related to the copy number of the short or long arm of the chromosome. Such unbalanced karyotypes could remain in the lymphocyte genome over further cell divisions for at least 120 h after culture initiation, indicating that these aberrant cells can survive and that they could pose a health risk.
Kusakabe
H
H
Laboratory of Cell Toxicology, Hatano Research Institute/Food and Drug Safety Center, Kanagawa, Japan.
Takahashi
T
T
Tanaka
N
N
D000328:Adult; D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects / Q000235:genetics; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000235:genetics; D016685:Mitomycin / Q000633:toxicity*; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin