IL-10 and trichosanthin inhibited surface molecule expression of antigen processing cells and T-cell proliferation.
OBJECTIVE
To study immunoinhibitory effects and preliminary mechanism of IL-10 and trichosanthin.


METHODS
Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analyzed by FACScan. B7-1 mRNA expression was detected with nested RT-PCR.


RESULTS
IL-10 2 mg.L-1 and trichosanthin 10 mg.L-1 inhibited B7-1 molecule expression. By contrast, they had not the same effects on ICAM-1. IL-10 and trichosanthin down-regulated LFA-1 expression, but had no regulatory effect on CD40. IL-10 and trichosanthin dramatically inhibited T-cell proliferation and IL-2 production. B7-1 mRNA expression was undetectable in APC treated with IL-10 and trichosanthin.


CONCLUSIONS
IL-10 and trichosanthin inhibit surface molecule expression on APC. They exert multiple immunoinhibitory effects.
Fan
Z S
ZS
Shanghai Institute of Immunology, Shanghai Second Medical University, China. aosz@server.shcnc.ac.cn
Ma
B L
BL
D000938:Antigen-Presenting Cells / Q000276:immunology*; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects*; D015978:Trichosanthin / Q000494:pharmacology*
D018122:B7-1 Antigen; D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D016753:Interleukin-10; D015978:Trichosanthin