title: The cytostome of Trypanosoma cruzi epimastigotes is associated with the flagellar complex.
abstract: Okuda, K., Esteva, M., Segura, E. L., and Bijovsky, A. T. 1999. The cytostome of Trypanosoma cruzi epimastigotes is associated with the flagellar complex. Experimental Parasitology 92, 223-231. Proliferative forms of Trypanosoma cruzi, amastigotes and epimastigotes, have a cytostome, a specialized structure formed by an invagination of the flagellar pocket's membrane surrounded by microtubules and frequently followed by a row of vesicles. All this assemblage penetrates deeply into the cytoplasm overpassing the nucleus. This structure, together with the flagellar pocket, appears to play an important role in the nutrition of the parasite. We demonstrated that the monoclonal antibody 2C4, made-up against isolated flagellar complex of T. cruzi epimastigotes, recognizes a protein doublet of 76 and 87 kDa in total epimastigotes homogenate. The 76-kDa polypeptide is enriched in the detergent-soluble fraction whereas the 87-kDa polypeptide is highly represented in the insoluble fractions and the purified flagella. Immuno-fluorescence assays show the antigen as a small spot at the flagellar pocket region. Immunogold labeling of ultrathin sections of epimastigote forms reveals gold particles at the opening of flagellar pocket, concentrated in the cytostome region. Immunocytochemistry of epimastigote whole-mount cytoskeletons reveals the labeling on an array of three to four microtubules that appears attached to flagellum, running in the direction of the nucleus. Ultrastructural observations have shown that the posterior region of isolated flagella, corresponding to the level of the flagellar pocket, possesses a microtubular structure compatible with that from the cytostome. The relationship between the cytostome, an endocytic organelle, and the flagellum is here described for the first time.
authors.affiliation: Departamento de Parasitologia, ICB-USP, Sāto Paulo, SP, 05508-900, Brazil.
authors.forename: K
authors.initials: K
authors.lastname: Okuda
authors.forename: M
authors.initials: M
authors.lastname: Esteva
authors.forename: E L
authors.initials: EL
authors.lastname: Segura
authors.forename: A T
authors.initials: AT
authors.lastname: Bijovsy
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003599:Cytoskeleton / Q000648:ultrastructure; D005407:Flagella / Q000648:ultrastructure*; D019084:Fluorescent Antibody Technique, Indirect; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure*; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000648:ultrastructure*