Relationship between cell migration and cell cycle during the initiation of epithelial to fibroblastoid transition.
The NBT-II rat bladder carcinoma cell line, which displays epithelial to mesenchymal transition or EMT in response to FGF-1 stimulation, was used to study the interrelationships between cell cycle and cell scattering and locomotion. Time-lapse video microscopy experiments were performed with asynchronous growing cells and lovastatin-arrested cells. FGF-1 stimulation induced cell movement in cells in all phases of the cell cycle, except G2 + M phase, in which cells did not respond to stimulation. The delay between cell stimulation and cell movement depended on the age of the cell at the beginning of cell stimulation: cells less than 4 h old when stimulated by FGF-1 had a 1-h delay whereas cells more than 4 h old had a 3-h delay. Cells stimulated before they were 4 h old were temporarily arrested in their cell cycle progression. Older cells underwent mitosis on schedule. Lovastatin-treated cells were shown to be synchronized in the G1 phase and to migrate simultaneously after FGF-1 stimulation. These results indicate that the G1 phase was a critical phase for FGF-1 induced cell migration during epithelial to fibroblastoid transition.
Bonneton
C
C
UM144: Compartimentation et Dynamique Cellulaires Centre National de la Recherche Scientifique et Institut Curie, Paris, France. cbonnet@curie.fr
Sibarita
J B
JB
Thiery
J P
JP
D000818:Animals; D002453:Cell Cycle; D002465:Cell Movement; D004847:Epithelial Cells / Q000187:drug effects; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005434:Flow Cytometry; D016193:G1 Phase; D006801:Humans; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014743:Videotape Recording
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1