Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice.
It is widely recognized that fluorescence techniques represent the most important future detection method for miniaturized ultra-high-throughput screening. However, such techniques encompass many different approaches, each with its own particular set of advantages and limitations. Here, the authors summarize each of the major fluorescence techniques and explain the underlying principles that form the basis for a theory-led strategy to readout selection and assay design.
Pope
AJ
Molecular Interactions and New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, UK CM19 5AW.
Haupts
UM
Moore
KJ