title: Interference of the antimicrobial peptide lactoferricin B with the action of various antibiotics against Escherichia coli and Staphylococcus aureus.
abstract: The antimicrobial peptide, lactoferricin, can be generated upon gastric pepsin cleavage of lactoferrin. We have examined the interaction of lactoferricin of bovine origin, Lf-cin B, with the antibiotics penicillin G, vancomycin, gentamicin, colistin, D-cycloserine and erythromycin against E. coli ATCC 25922 and Staphylococcus aureus ATCC 25923. We demonstrated synergism between Lf-cin B and erythromycin against E. coli, and partial synergism between Lf-cin B and penicillin G, vancomycin and gentamicin against E. coli. Only penicillin G acted in partial synergism with Lf-cin B against S. aureus. Lf-cin B antagonized vancomycin and gentamicin against S. aureus in low concentration. We conclude that Lf-cin B may facilitate the uptake of antibiotics across the cell envelope.
authors.affiliation: Department of Medical Microbiology, University Hospital, Tromsø, Norway.
authors.forename: L H
authors.initials: LH
authors.lastname: Vorland
authors.forename: S A
authors.initials: SA
authors.lastname: Osbakk
authors.forename: T
authors.initials: T
authors.lastname: Perstølen
authors.forename: H
authors.initials: H
authors.lastname: Ulvatne
authors.forename: O
authors.initials: O
authors.lastname: Rekdal
authors.forename: J S
authors.initials: JS
authors.lastname: Svendsen
authors.forename: T J
authors.initials: TJ
authors.lastname: Gutteberg
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002417:Cattle; D004347:Drug Interactions; D004357:Drug Synergism; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D005839:Gentamicins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007781:Lactoferrin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010400:Penicillin G / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*; D014640:Vancomycin / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; C082171:lactoferricin B; D004917:Erythromycin; D014640:Vancomycin; D007781:Lactoferrin; D010400:Penicillin G