title: Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer.
abstract: We demonstrated that infection of 17Cl-1 cells with the murine coronavirus mouse hepatitis virus (MHV) induced caspase-dependent apoptosis. MHV-infected DBT cells did not show apoptotic changes, indicating that apoptosis was not a universal mechanism of cell death in MHV-infected cells. Expression of MHV structural proteins by recombinant vaccinia viruses showed that expression of MHV E protein induced apoptosis in DBT cells, whereas expression of other MHV structural proteins, including S protein, M protein, N protein, and hemagglutinin-esterase protein, failed to induce apoptosis. MHV E protein-mediated apoptosis was suppressed by a high level of Bcl-2 oncogene expression. Our data showed that MHV E protein is a multifunctional protein; in addition to its known function in coronavirus envelope formation, it also induces apoptosis.
authors.affiliation: Department of Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA.
authors.forename: S
authors.initials: S
authors.lastname: An
authors.forename: C J
authors.initials: CJ
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: J L
authors.initials: JL
authors.lastname: Leibowitz
authors.forename: S
authors.initials: S
authors.lastname: Makino
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D061945:Caspase Inhibitors; D002460:Cell Line; D053938:DNA Fragmentation; D051379:Mice; D006517:Murine hepatitis virus / Q000378:metabolism* / Q000472:pathogenicity; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D061945:Caspase Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; D014759:Viral Envelope Proteins; C505409:Casp3 protein, mouse; D053148:Caspase 3