Permeation of dicarboxylic acids with different terminal position of two carboxylic groups through planar bilayer lipid membranes.
Electrically silent hydrogen ion fluxes across a planar bilayer lipid membrane (BLM) induced by an addition of dicarboxylic (DC) acids at one side of BLM are monitored by measuring pH changes in the unstirred layers near the BLM surface via recording protonophore-dependent potentials. Two groups of DC acids are studied: (1) 2-n-alkylmalonic acids with an alkyl chain of different length which carry both carboxylic groups at one terminus of the hydrocarbon chain (alpha,alpha-DC acids); and (2) dicarboxylic acids of different linear chain length having carboxylic groups at the opposite ends of the hydrocarbon chain (alpha,omega-DC acids). It is shown that the pH optimum of hydrogen ion fluxes for the DC acids is shifted considerably to acidic pH values compared to monocarboxylic acids and is located near pH 5. For both types of DC acids at pH&z. Lt;5, the total transport is limited by diffusion of the anionic forms of the acids across the unstirred layers, while at pH&z.Gt;5 the transport is limited by diffusion of the neutral form across the membrane. The fluxes of alpha,alpha-DC acids are similar to those of alpha,omega-DC acids provided that the acids have the similar number of carbon atoms, the fluxes grow with the increase in the chain length of the alkyl radical.
Evtodienko
V Y
VY
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation.
Bondarenko
D I
DI
Antonenko
Y N
YN
D003998:Dicarboxylic Acids / Q000737:chemistry*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D017136:Ion Transport; D008051:Lipid Bilayers / Q000737:chemistry*; D010539:Permeability; D010743:Phospholipids / Q000737:chemistry
D003998:Dicarboxylic Acids; D008051:Lipid Bilayers; D010743:Phospholipids