title: Antigenic relatedness of selected flaviviruses: study with homologous and heterologous immune mouse ascitic fluids.
abstract: The antigenic relationship of 9 flaviviruses, Yellow fever (YF), Wesselsbron (WSL), Uganda S (UGS), Potiskum (POT), West Nile (WN), Banzi (BAN), Zika (ZK), Dengue type 1 (DEN-1) and Dengue type 2 (DEN-2), was assessed by cross-haemagglutination-inhibition (Cross-HI) and cross-complement fixation (Cross-CF) reactions between each of the viruses and their homologous immune mouse ascitic fluids. Titre ratios were calculated using the heterologous and homologous titres. Cross-CF reactions revealed wider antigenic variations among viruses than Cross-HI reactions. There was no significant antigenic variation between WSL, POT and YF viruses using either of those methods. However, definite differences in antigenicity were observed between them and UGS, BAN and ZK viruses. There were no significant differences between UGS, BAN and ZK or between DEN-1 and DEN-2. The serological relationship among flaviviruses is important in establishing diagnosis and epidemiology of these infections in Africa.
authors.affiliation: Department of Veterinary Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.
authors.forename: S S
authors.initials: SS
authors.lastname: Baba
authors.forename: A H
authors.initials: AH
authors.lastname: Fagbami
authors.forename: O D
authors.initials: OD
authors.lastname: Olaleye
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000940:Antigenic Variation / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology*; D001202:Ascitic Fluid / Q000821:virology*; D003168:Complement Fixation Tests; D003429:Cross Reactions; D005416:Flavivirus / Q000145:classification / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D051379:Mice
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral