Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant.
A heterotrophic bacterial strain TL1T capable of aerobic denitrification was previously enriched in continuous culture from a landfill leachate treatment plant and isolated as a pure culture. The taxonomic position of this isolate within the beta-subclass of the Proteobacteria was determined by 16S rDNA sequence analysis and by conventional taxonomy including substrate spectrum, quinone type (ubiquinone Q-8) and cellular fatty acid composition. Detection of the specific polyamine 2-hydroxyputrescine supports the membership of strain TL1T in the beta-subclass of the Proteobacteria. The results of 16S rDNA sequencing showed that the strain clustered with, but was separate from, Thauera aromatica and Thauera selenatis. DNA-DNA hybridization experiments indicated that the new isolate represents a new species of the genus, for which the name Thauera mechernichensis is proposed; the type strain is DSM 12266T.
Scholten
E
E
Institut für Mikrobiologie, Universität Hannover, Germany.
Lukow
T
T
Auling
G
G
Kroppenstedt
R M
RM
Rainey
F A
FA
Diekmann
H
H
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D009565:Nitrate Reductases; D050901:Nitrate Reductase