title: Calf primary hepatocyte culture as a tool for anabolic steroid metabolism studies.
abstract: Calf hepatocyte cultures were developed for the predictive analysis of in vivo xenobiotic biotransformations. The goal of this work was to show the feasibility of a system based on calf primary hepatocyte cultures using radiolabelled molecules to study xenobiotic metabolism rather than to study exhaustively the metabolism of a particular steroid. 19-Nortestosterone was chosen as a model substrate because of its relatively well known metabolism in the bovine species. Incubation of a mixture of tritiated and non-tritiated 19-nortestosterone was investigated in cell culture in order to target the biosynthesized metabolites. After extraction and purification, some of the in vitro metabolite structures were elucidated by GC-MS and compared with the main urinary metabolites detected in vivo after intramuscular injection of 19-nortestosterone into a calf. 19-Norepitestosterone, the main in vivo metabolite, was identified in vitro. However, the main in vitro metabolites were mostly in an oxidized form (4-estrene-3,17-dione, hydroxy-4-estrene-3,17-dione).
authors.affiliation: LDH-LNR, Ecole Nationale Vétérinaire (Ministère de l'Agriculture), Nantes, France.
authors.forename: A S
authors.initials: AS
authors.lastname: Clouet
authors.forename: B
authors.initials: B
authors.lastname: Le Bizec
authors.forename: F
authors.initials: F
authors.lastname: Boerlen
authors.forename: F
authors.initials: F
authors.lastname: Monteau
authors.forename: F
authors.initials: F
authors.lastname: André
mesh_terms: D045930:Anabolic Agents / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000378:metabolism*; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000378:metabolism; D000077603:Nandrolone Decanoate; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D045930:Anabolic Agents; D015262:Xenobiotics; D009277:Nandrolone; D000077603:Nandrolone Decanoate