title: Hybrid Capture II-based human papillomavirus detection, a sensitive test to detect in routine high-grade cervical lesions: a preliminary study on 1518 women.
abstract: Hybrid Capture II (HC-II) is a commercial human papillomavirus (HPV) detection test designed to detect 18 HPV types divided into high-risk and low-risk groups. We have tested 1647 scrapes from 1518 unselected women attending routine cytological screening by this assay for the detection of histologically proven high-grade lesions. The reliability of this test was also evaluated on 117 fresh cone biopsy samples. HPV DNA has been detected in 400 scrapes (24.3%), 296 containing a high-risk HPV (18.0%). All the smears evocative of high-grade lesions were positive for high-risk HPV, and high-risk HPV were detected in all the 34 cases presenting a histologically proven high-grade lesion and in 68 (97.1%) of the 70 cone biopsy samples showing a high-grade lesion or an invasive carcinoma. Thus, the sensitivity was superior to the sensitivity of cytology (85.3%). Nevertheless, the quantitative approach provided by the HC-II assay for the assessment of the viral load could not clearly distinguish among cases with or without high-grade lesions. Thus this assay is recommended for the screening of high-grade lesions on a large scale, in association with classic cytology.
authors.affiliation: Laboratoire Pol Bouin, CHU de Reims, France.
authors.forename: C
authors.initials: C
authors.lastname: Clavel
authors.forename: M
authors.initials: M
authors.lastname: Masure
authors.forename: J P
authors.initials: JP
authors.lastname: Bory
authors.forename: I
authors.initials: I
authors.lastname: Putaud
authors.forename: C
authors.initials: C
authors.lastname: Mangeonjean
authors.forename: M
authors.initials: M
authors.lastname: Lorenzato
authors.forename: R
authors.initials: R
authors.lastname: Gabriel
authors.forename: C
authors.initials: C
authors.lastname: Quereux
authors.forename: P
authors.initials: P
authors.lastname: Birembaut
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral