title: In vitro cytotoxicity of fibre-polymethyl methacrylate composite used in dentures.
abstract: The aim of this study was to determine the in vitro cytotoxicity of fibre-polymethyl methacrylate (PMMA) composite used in denture bases. Both heat-cured and autopolymerized PMMA were tested as the matrix of the fibre composite or as a plain denture polymer. The fibres tested included unsized E-glass and silica fibres, silica fibres which were sized with a silane coupling agent only, as well as commercially epoxy-silane or methacrylate-chrome complex sized E-glass fibres. The indirect contact cytotoxicity test by agar diffusion was carried out according to the international standard ISO 10993-5 after storing the test specimens in water for 24 h. PVC plastic was used as a positive control and polyethylene plastic was used as a negative control. Within the limitations of the agar diffusion test, it can be concluded that neither the unreinforced PMMA nor the fibre composite made from PMMA and fibres was cytotoxic.
authors.affiliation: NIOM-Scandinavian Institute of Dental Materials, Haslum, Norway.
authors.forename: P K
authors.initials: PK
authors.lastname: Vallittu
authors.forename: K
authors.initials: K
authors.lastname: Ekstrand
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D017608:Chromium Compounds / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D003819:Denture Bases; D004853:Epoxy Resins / Q000737:chemistry; D005347:Fibroblasts / Q000473:pathology; D005898:Glass / Q000737:chemistry; D006358:Hot Temperature; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D010969:Plastics / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011143:Polyvinyl Chloride / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D017608:Chromium Compounds; D003188:Composite Resins; D004853:Epoxy Resins; D008689:Methacrylates; D010969:Plastics; D011095:Polyethylenes; D011108:Polymers; D012821:Silanes; C009753:fiberglass; D012822:Silicon Dioxide; D011143:Polyvinyl Chloride; D019904:Polymethyl Methacrylate