[Rapid detection of trisomy 21 by quantitative polymerase chain reaction].
OBJECTIVE
Studying gene diagnosis of trisomy 21 by means of quantitative polymerase chain reaction(PCR).


METHODS
Polymorphic short tandem repeat(STR) at D21S11 served as the gene marker, and a pair of primers was synthesized. The samples from 11 normal subjects and 28 cases of trisomy 21 were analyzed by quantitative PCR.


RESULTS
10 normal subjects showed two bands with a ratio of 1:1, and a subject showed one band. Of 28 cases of trisomy 21, 24 cases showed two bands with a ratio of 2:1, 3 cases showed three bands with a ratio of 1:1:1, and 1 case showed one band.


CONCLUSIONS
Polymorphic STR at D21S11 is a valuable gene marker for diagnosis of trisomy 21. Trisomy 21 can be diagnosed rapidly and accurately within 24 hours by quantitative PCR.
Jin
C
C
Department of Medical Genetics, China Medical University, Shenyang 110001 P.R.China. Klsun@IRIS.CMU.EDU.CN
Zhang
L
L
Wang
R
R
Yu
H
H
Jiang
L
L
Lin
C
C
Li
F
F
Sun
K
K
D004314:Down Syndrome / Q000175:diagnosis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*