Effect of recombinant human fibronectin polypeptide CH50 on growth and metastasis of melanoma.
OBJECTIVE
To study the effect of recombinant human fibronectin polypeptide CH50 on murine melanoma growth and metastasis, and its antitumor mechanism.


METHODS
Mouse and melanoma B16 cell tests were used to observe antitumor effect and mechanism of CH50.


RESULTS
CH50 markedly inhibited melanoma growth and experimental lung metastasis. The melanoma weight was reduced from (2.3 +/- 1.2) g of control group to (0.7 +/- 0.8) g of test group (P < 0.05). CH50 at 0.125, 0.25, 0.5 mg/mouse reduced melanoma lung metastatic colonies from 87 +/- 49 of control group to 34 +/- 6, 14 +/- 12, 4 +/- 2, respectively. CH50 adhered to melanoma B16 cells and inhibited adhesion of B16 cells to laminin. CH50 enhanced the cytotoxicity of macrophages to melanoma B16 cells.


CONCLUSIONS
CH50 inhibited tumor growth and metastasis of murine melanoma. The antitumor effect of CH50 is related to its adhesion ability to melanoma B16 cells and enhancing macrophage cytotoxicity.
Fan
Q
Q
Department of Medical Molecular Biology, Tongji Medical University, Wuhan, China.
Feng
Z H
ZH
Zhang
G M
GM
Zhang
H
H
Cao
H Q
HQ
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology* / Q000627:therapeutic use; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D017737:Macrophages, Peritoneal / Q000276:immunology; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000494:pharmacology / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D010446:Peptide Fragments; D011994:Recombinant Proteins