Role of nitric oxide on GABA, glutamic acid, activities of GABA-T and GAD in rat brain cerebral cortex.
The results of the present study clearly shows that a correlation exists between nitric oxide (NO) and gamma-aminobutyric acid transaminase (GABAT-T) activity as well as gamma-aminobutyric acid (GABA), glutamic acid and the activity of glutamic acid decarboxylase (GAD). Supporting of this 10 min after the administration of L-Arginine (L-Arg) increased GABA concentration and diminished the activity of GABA-T. There was no change in GAD activity and glutamic acid level. Administration of convulsion inducing agent Picrotoxin (PCT) decreased the NO concentration in the brain and enhanced the activity of GABA-T, and the fact that the NOS inhibitor (N(G)-nitro-L-Arg methyl ester (L-NAME) diminished the activity of NOS and increased the activity of GABA-T provide another support for the involvement of NO on GABA-T activity. The present study clearly showed that high concentrations of NO in the brain suppresses the activity of GABA-T.
Jayakumar
A R
AR
Bio-organic and Neurochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai - 600 020, India.
Sujatha
R
R
Paul
V
V
Asokan
C
C
Govindasamy
S
S
Jayakumar
R
R
D000612:4-Aminobutyrate Transaminase / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology*; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000612:4-Aminobutyrate Transaminase; D001120:Arginine; D005968:Glutamate Decarboxylase; D018698:Glutamic Acid; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide; D010852:Picrotoxin; D005680:gamma-Aminobutyric Acid