Biological effects of man-made mineral fibers (II)--their genetic damages examined by in vitro assay.
In order to study and compare genetic damage induced by 10 kinds of man-made mineral fibers (JFM fibers) in cells, human lung epithelial cells (A549) were exposed to JFM fibers and chrysotile for 1 h, then single-cell gel electrophoresis (SCGE) assay was used to detect DNA strand breaks, DNA-DNA interstrand crosslink and the ability of DNA to repair; The results showed that all 10 JFM fibers could induce DNA strand breaks, DNA-DNA interstrand crosslinks and inhibit the ability of DNA repair. When human embryo lung (HEL) cells were exposed to JFM fibers and chrysotile for 24 h respectively, the chromosomal aberration was analyzed and the results showed that chrysotile and most of JFM fibers at 5.0 micrograms/ml induced structural chromosomal aberration, but all of these effects were lower than that of chrysotile and were different among them, suggesting that 10 types of JFM fibers had genotoxicity with different degree in vitro, but all of them were lower than that of chrysotile.
Wang
Q E
QE
Department of Occupational Health, Beijing Medical University, P.R. China.
Han
C H
CH
Yang
Y P
YP
Wang
H B
HB
Wu
W D
WD
Liu
S J
SJ
Kohyama
N
N
D000704:Analysis of Variance; D001194:Asbestos / Q000633:toxicity; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D019016:Mineral Fibers / Q000633:toxicity*
D019016:Mineral Fibers; D001194:Asbestos; D004247:DNA