[Heterologous expression of murine lymphotoxins in Escherichia coli and preparation of antibodies].
Genes encoding fragments of polypeptide chains of murine lymphotoxins (LT), namely, LT-alpha truncated from the N-terminus and the LT-beta extracellular domain, containing N-terminal hepta- and hexahistidine epitopes, respectively, were expressed in E. coli cells. The recombinant proteins purified by metallochelate chromatography were used to obtain polyclonal antibodies that specifically recognize murine LT.
Korobko
V G
VG
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Boĭchenko
V E
VE
Kuprash
D V
DV
Turetskaia
R L
RL
Nedospasov
S A
SA
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006639:Histidine / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000276:immunology; D051379:Mice; D010957:Plasmids
D000906:Antibodies; D000939:Epitopes; D008233:Lymphotoxin-alpha; D006639:Histidine
Geterologicheskaia ékspressiia limfotoksinov myshi v kletkakh Escherichia coli i poluchenie antitel k nim.