Reasons and limits of substrate activity of modified L-dNTP in DNA biosynthesis.
Theoretical and experimental analysis of interaction of modified D- and L- dNTP as substrates for template-dependent and template-independent DNA polymerases was performed. It is shown that if the modified nucleoside 5'-triphosphates do not contain a substituent in position 3' DNA chains can be extended by both strereoisomeric series with the same kinetic parameters. But the presence of even a 3'-hydroxy group in L-dNTP prevents their incorporation into the DNA chain.
Krayevsky
A A
AA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Dyatkina
N B
NB
Semizarov
D G
DG
Victorova
L S
LS
Shirokova
E A
EA
Theil
F
F
Von Janta Lipinski
M J
MJ
Gosselin
G
G
Imbach
J L
JL
D004261:DNA Replication; D009711:Nucleotides / Q000378:metabolism*; D013379:Substrate Specificity
D009711:Nucleotides