Estradiol increases the production of sex hormone-binding globulin but not insulin-like growth factor binding protein-1 in cultured human hepatoma cells.
OBJECTIVE
To study the effects of E2 on insulin-like growth factor binding protein-1 (IGFBP-1) and sex hormone-binding globulin (SHBG) production with cultured human HepG2 hepatoma cells.


METHODS
Experimental cell culture.


METHODS
Academic medical center.


METHODS
None.


METHODS
Addition of E2 to cell culture medium.


METHODS
Intracellular and released concentrations of IGFBP-1 and SHBG.


RESULTS
Estradiol did not affect the intracellular or extracellular IGFBP-1 concentration, whereas the intracellular SHBG concentration increased significantly in response to 0.5-2.5 microM of E2.


CONCLUSIONS
Whereas the two binding proteins share a number of regulatory factors, their regulation by E2 is dissimilar in human hepatoma cells. Estradiol does not affect the intracellular or secreted IGFBP-1 concentration, but it does increase the production of SHBG.
Kalme
T
T
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.
Loukovaara
M
M
Koistinen
R
R
Koistinen
H
H
Angervo
M
M
Leinonen
P
P
Seppälä
M
M
D006528:Carcinoma, Hepatocellular; D004273:DNA, Neoplasm / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis*; D007700:Kinetics; D008113:Liver Neoplasms; D012738:Sex Hormone-Binding Globulin / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D018970:Insulin-Like Growth Factor Binding Protein 1; D012738:Sex Hormone-Binding Globulin; D004958:Estradiol