title: Prevalence of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations in a Greek population of blood donors.
abstract: The pathogenesis of venous thrombosis involves the interaction of genetic and environmental factors. In order to estimate the frequency of the factor V Leiden, the prothrombin G20210A, and the MTHFR C677T mutations in the Greek population, we analyzed 160 healthy Greek blood donors by PCR amplification and detected allele frequencies of 2.5%, 2.2%, and 35.3%, respectively. The allele frequencies were compared with reported frequencies of other populations of southern Europe. The identification of these common genetic risk factors for thrombosis should enable easy DNA diagnosis and carrier detection in a high proportion of cases and will contribute to a better understanding of the interaction of genetic and environmental risk factors.
authors.affiliation: Department of Genetics and Molecular Biology, Mitera Maternity and Surgical Center, Marousi, Greece.
authors.forename: T
authors.initials: T
authors.lastname: Antoniadi
authors.forename: T
authors.initials: T
authors.lastname: Hatzis
authors.forename: C
authors.initials: C
authors.lastname: Kroupis
authors.forename: E
authors.initials: E
authors.lastname: Economou-Petersen
authors.forename: M B
authors.initials: MB
authors.lastname: Petersen
mesh_terms: D000328:Adult; D000483:Alleles; D001782:Blood Donors; D005165:Factor V / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D006115:Greece; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D008875:Middle Aged; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics* / Q000302:isolation & purification; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011516:Prothrombin / Q000235:genetics* / Q000302:isolation & purification; D012307:Risk Factors; D020246:Venous Thrombosis / Q000209:etiology / Q000235:genetics*
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin; D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)