title: Effect of pH on Ki-67 antigen retrieval in renal cell carcinoma.
abstract: The efficacy of Ki-67 antigen retrieval (AR) was assessed in 20 formalin fixed-paraffin embedded specimens of renal cell carcinoma using three solutions with different pH values. The AR was performed by means of microwave oven heating up to 97 degrees C for 15 minutes. Better results were obtained using solutions with pH values 8.0 and 9.9 compared with AR in a solution of pH 6.0, which is routinely used in daily pathological practice. Individual testing of various AR conditions is recommended to obtain the best immunohistochemical results.
authors.affiliation: Institute of Pathology, Medical Faculty, University of Ljubljana, Slovenia.
authors.forename: A
authors.initials: A
authors.lastname: Masera
authors.forename: O
authors.initials: O
authors.lastname: Stanisa
authors.forename: Z
authors.initials: Z
authors.lastname: Ovcak
authors.forename: A
authors.initials: A
authors.lastname: Cör
mesh_terms: D002292:Carcinoma, Renal Cell / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000276:immunology*
chemical_list: D019394:Ki-67 Antigen