title: Detection of human papilloma virus type 56 in extragenital Bowen's disease.
abstract: A case of Bowen's disease arising on the medial part of the first metatarsal bone of an 81-year-old Japanese woman is described. Histopathologically, proliferation of atypical cells was found throughout the epidermis. Electronmicroscopy revealed virus particles 40-50 nm in diameter in the nuclei of tumour cells at the granular cells just on or below the horny layer. Positive bands were obtained by polymerase chain reaction using a consensus primer of human papilloma virus L1 portion. Sequencing analysis of the amplified DNA revealed the same base sequences and homology as human papilloma virus 56. To the best of our knowledge, this case is the first report in which human papilloma virus 56 was found in a case of extragenital Bowen's disease. We consider it important to understand that human papilloma virus 56, often found in cervical lesions, can be detected in extragenital Bowen's diseases.
authors.affiliation: Department of Dermatology, Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uezato
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: S T
authors.initials: ST
authors.lastname: Ramuzi
authors.forename: N M
authors.initials: NM
authors.lastname: Khaskhely
authors.forename: T
authors.initials: T
authors.lastname: Nagata
authors.forename: Y
authors.initials: Y
authors.lastname: Nagamine
authors.forename: S
authors.initials: S
authors.lastname: Nonaka
authors.forename: T
authors.initials: T
authors.lastname: Asato
authors.forename: M
authors.initials: M
authors.lastname: Oshiro
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001913:Bowen's Disease / Q000473:pathology* / Q000821:virology; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000473:pathology* / Q000821:virology; D014034:Toes; D014412:Tumor Virus Infections / Q000150:complications / Q000473:pathology*