Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells.
The embryonic lethal abnormal vision (ELAV)-like proteins are mRNA-binding proteins that regulate mRNA stability. The neuronal members of this family are required for neuronal differentiation. We identified the binding region of purified HuD protein to a target neuronal mRNA encoding for the tau microtubule-associated protein and demonstrated an in vivo interaction between the ELAV-like protein and its target tau mRNA. We show that treatment of neuronal cells with antisense oligodeoxynucleotides directed against HuD blocks the induction of neurite outgrowth and decreases the levels of tau mRNAs, indicating that the ELAV-like proteins are required for neuronal differentiation.
Aranda-Abreu
G E
GE
Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel.
Behar
L
L
Chung
S
S
Furneaux
H
H
Ginzburg
I
I
D000818:Animals; D001483:Base Sequence; D029742:Caenorhabditis elegans Proteins; D002454:Cell Differentiation / Q000502:physiology; D016764:Cell Polarity; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D006801:Humans; D008870:Microtubules / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016716:PC12 Cells; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016875:tau Proteins / Q000235:genetics*
D029742:Caenorhabditis elegans Proteins; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; C473926:EXC-7 protein, C elegans; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D016875:tau Proteins