[Isolation, crystallization, and preliminary x-ray study of a Fab fragment of monoclonal antibody against human interleukin-2 and its complex with antigenic peptide].
Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human interleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelectric focusing method. Various monocrystals of these free Fab-fragments and their complexes with the antigenic peptide corresponding to the 59-72 sequence of interleukin-2 were obtained. These were shown to be suitable for X-ray and were preliminarily studied by X-ray.
Mikhaĭlova
I Iu
IIu
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Mareeva
T Iu
TIu
Tsygannik
I N
IN
Mikhaleva
I I
II
Onoprienko
L V
LV
Vikhrov
A A
AA
Markvicheva
E A
EA
Panghorn
W
W
Duax
W
W
Nesmiianov
V A
VA
Pletnev
V Z
VZ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000302:isolation & purification*; D007376:Interleukin-2 / Q000276:immunology*; D007525:Isoelectric Focusing; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2; D010446:Peptide Fragments
Poluchenie, kristallizatsiia i predvaritel'nye rentgenostrukturnye issledovaniia Fab-fragmenta monoklonal'nogo antitela k interleĭkinu-2 cheloveka i ego kompleksa s antigennym peptidom.