Fusarium sp. growth inhibition by wheat germ agglutinin.
The antifungal role of wheat germ agglutinin (WGA) isolated from a Romanian dihaploid variety of wheat against two pathogenic fungal species of Fusarium, F. graminearum and F. oxysporum, is demonstrated. WGA was prepared from unprocessed wheat germs by a new purification procedure using chitin and fetuin-Sepharose as affinity chromatography supports. SDS-PAGE and chitinase assay showed that the WGA preparation migrated as a single protein band and was devoid of any contaminating enzyme chitinase, well known for its antifungal effects. Based on its affinity for N-acetylglucosamine residues, WGA binding to the chitin-containing walls of the fungi was detected by fluorescence microscopy using WGA coupled with fluorescein isothiocyanate (FITC). In vitro testing of WGA action on early developmental stages of both fungal strains resulted in various modifications of the germ tubes, visualised by light microscopy: swelling, vacuolation of the cellular content and lysis of cell walls. Viability tests performed on potato tuber slices showed that the microbial infection was prevented from spreading by pretreatment of the fungal suspension with WGA.
Ciopraga
J
J
Institute of Biochemistry, Romanian Academy, Spl. Independentei 296, P.O. Box 17-37, 77700, Bucharest, Romania. ciopraga@biochim.ro
Gozia
O
O
Tudor
R
R
Brezuica
L
L
Doyle
R J
RJ
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects; D002846:Chromatography, Affinity; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005670:Fusarium / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D008856:Microscopy, Fluorescence; D014909:Wheat Germ Agglutinins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000935:Antifungal Agents; D014909:Wheat Germ Agglutinins; D016650:Fluorescein-5-isothiocyanate