Anchoring of canine linkage groups with chromosome-specific markers.
A high-resolution genetic map with polymorphic markers spaced frequently throughout the genome is a key resource for identifying genes that control specific traits or diseases. The lack of rigorous selection against genetic disorders has resulted in many breeds of dog suffering from a very high frequency of genetic diseases, which tend to be breed-specific and usually inherited as autosomal recessive or apparently complex genetic traits. Many of these closely resemble human genetic disorders in their clinical and pathologic features and are likely to be caused by mutations in homologous genes. To identify loci important in canine disease genes, as well as traits associated with morphological and behavioral variation, we are developing a genetic map of the canine genome. Here we report on an updated version of the canine linkage map, which includes 341 mapped markers distributed over the X and 37 autosomal linkage groups. The average distance between markers on the map is 9.0 cM, and the linkage groups provide estimated coverage of over 95% of the genome. Fourteen linkage groups contain either gene-associated or anonymous markers localized to cosmids that have been assigned to specific canine chromosomes by FISH. These 14 linkage groups contain 150 microsatellite markers and allow us to assign 40% of the linkage groups to specific canine chromosomes. This new version of the map is of sufficient density and characterization to initiate mapping of traits of interest.
Werner
P
P
Section of Medical Genetics and Center for Comparative Medical Genetics, University of Pennsylvania School of Veterinary Medicine, VHUP Room 4030, 3900 Delancey Street, Philadelphia, Pennsylvania 19104-6010, USA.
Mellersh
C S
CS
Raducha
M G
MG
DeRose
S
S
Acland
G M
GM
Prociuk
U
U
Wiegand
N
N
Aguirre
G D
GD
Henthorn
P S
PS
Patterson
D F
DF
Ostrander
E A
EA
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015342:DNA Probes; D005819:Genetic Markers