Cloning and sequencing of the beta-fructofuranosidase gene from Bacillus sp. V230.
The beta-fructofuranosidase gene (bff) from Bacillus sp. V230 has been cloned in Escherichia coli and its nucleotide sequence has been analyzed. The product of bff consists of a signal sequence of 32 amino acid (a.a.) residues for secretion and 455 a.a. residues of the extracellular beta-fructofuranosidase. The a.a. sequence of the bff product has similarities with those of the Bacillus subtilis levanscrase (63.7% identity), the Streptococcus mutans fructosyltransferase (33.7%), and the Zymomonas mobilis levanscrase and beta-fructofuranosidase (15%).
Kurimoto
M
M
Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Tsusaki
K
K
Kubota
M
M
Fukuda
S
S
Tsujisaka
Y
Y
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D043324:beta-Fructofuranosidase
D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase