Interactions of calf spleen purine nucleoside phosphorylase with antiviral acyclic nucleoside phosphonate inhibitors: kinetics and emission studies.
Association between calf spleen purine nucleoside phosphorylase and a series of phosphonylalkoxyalkyl derivatives of purine bases was studied by inhibition kinetics and fluorimetric titrations. Dissociation constants, determined by fluorimetric titration in phosphate-free conditions, were lower than inhibition constants in 1 mM phosphate, and inhibition was still weaker in 50 mM phosphate, in accord with the postulated bisubstrate analogue character of this class of inhibitors.
Wierzchowski
J
J
Department of Organic Chemistry, Pedagogical University, Siedlce, Poland.
Kulikowska
E
E
Bzowska
A
A
Holy
A
A
Magnowska
L
L
Shugar
D
D
D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D009705:Nucleosides / Q000737:chemistry / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000201:enzymology*
D004791:Enzyme Inhibitors; D009705:Nucleosides; D009943:Organophosphorus Compounds; D011683:Purine-Nucleoside Phosphorylase