Lag-burst kinetics in phospholipase A(2) hydrolysis of DPPC bilayers visualized by atomic force microscopy.
The lag-burst phenomenon in the phospholipase A(2) mediated hydrolysis of phospholipid bilayers is for the first time demonstrated in an atomic force microscopy (AFM) study. Simultaneous AFM measurements of the degree of bilayer degradation and the physical-chemical state of the membrane reveals growing nanoscale indentations in the membrane during the lag phase. It is argued that these indentations are domains of hydrolysis products (lysoPC/PC) which eventually trigger the burst. The rate of the rapid hydrolysis following the burst is found to be proportional to the length of the edge between membrane adsorbed and desorbed to the mica base. The observed maximal rate of membrane degradation is approx. 0.2 mmol lipid/min/mol lipase in solution.
Nielsen
L K
LK
Department of Chemistry, Technical University of Denmark, DK-2800, Lyngby, Denmark.
Risbo
J
J
Callisen
T H
TH
Bjørnholm
T
T
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D010741:Phospholipases A / Q000378:metabolism*; D012910:Snake Venoms / Q000201:enzymology; D012996:Solutions
D008051:Lipid Bilayers; D012910:Snake Venoms; D012996:Solutions; D015060:1,2-Dipalmitoylphosphatidylcholine; D010741:Phospholipases A