Embryonic stem cell-derived glial precursors: a source of myelinating transplants.
Self-renewing, totipotent embryonic stem (ES) cells may provide a virtually unlimited donor source for transplantation. A protocol that permits the in vitro generation of precursors for oligodendrocytes and astrocytes from ES cells was devised. Transplantation in a rat model of a human myelin disease shows that these ES cell-derived precursors interact with host neurons and efficiently myelinate axons in brain and spinal cord. Thus, ES cells can serve as a valuable source of cell type-specific somatic precursors for neural transplantation.
Brüstle
O
O
Department of Neuropathology, University of Bonn Medical Center, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany. brustle@uni-bonn.de
Jones
K N
KN
Learish
R D
RD
Karram
K
K
Choudhary
K
K
Wiestler
O D
OD
Duncan
I D
ID
McKay
R D
RD
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D002552:Cerebral Ventricles / Q000196:embryology / Q000601:surgery; D002549:Diffuse Cerebral Sclerosis of Schilder / Q000235:genetics / Q000628:therapy*; D004622:Embryo, Mammalian / Q000166:cytology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D004676:Myelin Basic Protein / Q000096:biosynthesis; D018991:Myelin Proteolipid Protein / Q000096:biosynthesis / Q000235:genetics; D009186:Myelin Sheath / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D051381:Rats; D013116:Spinal Cord; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
D006133:Growth Substances; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein