Low-tech electrophoresis, small but beautiful, and effective: electrophoretic titration curves of proteins.
Migration across a stationary pH gradient results in the electrophoretic titration of a protein's dissociable groups. From the resulting curves, some properties of the protein may be derived, including overall amino acid composition and type of mutation between polymorphic variants, as well as range of stability or, for enzymes, of catalytic activity. Analysis with this technique is a stringent purity criterion; other applications allow the study of interacting systems and the planning of chromatographic fractionations based on differences in surface charge.
Gianazza
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Istituto di Scienze Farmacologiche, Facoltà di Farmacia, Università degli Studi di Milano, Italy. elisabetta.gianazza@unimi.it
Miller
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Eberini
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Castiglioni
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D000596:Amino Acids / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods; D011489:Protein Denaturation; D012709:Serum Albumin / Q000032:analysis; D017186:Titrimetry / Q000379:methods*; D014508:Urea / Q000737:chemistry
D000596:Amino Acids; D012709:Serum Albumin; D014508:Urea