title: Solution structure of dynorphin A (1-17): a NMR study in a cryoprotective solvent mixture at 278 K.
abstract: Dynorphin A, the endogenous agonist for the kappa opioid receptor, has been studied by NMR spectroscopy in methanol, acetonitrile, DMSO and in mixtures of hexafluoroacetone/water and DMSO/water. NMR data in the DMSO/water cryomixture at 278 K are consistent with a conformer in which the N-terminal part, like the corresponding message domain of enkephalins, is poorly ordered, whereas the C-terminal part is folded in a loop centred around Pro10. The folded structure of the C-terminal part (address moiety) may shed light on the role of the essential residues Arg7, Lys11 and Lys13.
authors.affiliation: Istituto Chimica MIB del CNR, Napoli, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Spadaccini
authors.forename: O
authors.initials: O
authors.lastname: Crescenzi
authors.forename: D
authors.initials: D
authors.lastname: Picone
authors.forename: T
authors.initials: T
authors.lastname: Tancredi
authors.forename: P A
authors.initials: PA
authors.lastname: Temussi
mesh_terms: D000595:Amino Acid Sequence; D003080:Cold Temperature; D004399:Dynorphins / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011957:Receptors, Opioid / Q000737:chemistry; D017473:Receptors, Opioid, kappa / Q000737:chemistry; D017450:Receptors, Opioid, mu / Q000737:chemistry; D012996:Solutions; D012997:Solvents; D000094942:Nociceptin Receptor
chemical_list: D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D012996:Solutions; D012997:Solvents; D004399:Dynorphins; D000094942:Nociceptin Receptor; C000722283:OPRL1 protein, human