Release of serotonin in the locus coeruleus of normotensive and spontaneously hypertensive rats (SHR).
The aim of the present work was to clarify whether differences exist between the release of endogenous serotonin in the locus coeruleus of normotensive and hypertensive rats. The locus coeruleus was superfused with artificial cerebrospinal fluid (aCSF) through a push-pull cannula and serotonin and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) were determined in the superfusate by HPLC combined with electrochemical detection. Compared with normotensive Wistar-Kyoto (WKY) rats, the basal release rate of serotonin in the locus coeruleus of spontaneously hypertensive rats (SHR) was increased more than twofold. Intravenous infusion of noradrenaline (4 microg/kg min) increased mean arterial blood pressure to the same extent in hypertensive and normotensive rats. The pressor response was associated with an increased serotonin release. In WKY rats, the release of serotonin in the locus coeruleus evoked by noradrenaline infusion was more pronounced than in SHR. In WKY rats, intravenous infusion of sodium nitroprusside (150 microg/kg min) led to a fall in blood pressure which was less pronounced and lasted shorter than in SHR. The depressor response was associated with decreased serotonin release. In WKY rats, the decrease in serotonin release evoked by sodium nitroprusside was more pronounced and lasted longer than in SHR. Neither noradrenaline nor sodium nitroprusside influenced the outflow of 5-HIAA. The sensory stimuli noise and tail pinch led to a slight rise in arterial blood pressure which was similar in WKY rats and SHR. These stimuli enhanced the release rate of serotonin and the outflow of 5-HIAA to the same extent in the locus coeruleus of normotensive and hypertensive rats. The findings suggest that the enhanced release of serotonin in the locus coeruleus of genetically hypertensive rats reflects a mechanism counteracting the disturbed blood pressure homeostasis. Stressors influence blood pressure and release of serotonin in the locus coeruleus of SHR and WKY rats to the same extent.
Kaehler
S T
ST
Institut für Pharmakologie und Toxikologie der Universität Innsbruck, Austria.
Singewald
N
N
Philippu
A
A
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D008125:Locus Coeruleus / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D009622:Noise / Q000009:adverse effects; D009638:Norepinephrine / Q000494:pharmacology; D010812:Physical Stimulation; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012701:Serotonin / Q000378:metabolism*; D013566:Sympathomimetics / Q000494:pharmacology
D020030:Nitric Oxide Donors; D013566:Sympathomimetics; D009599:Nitroprusside; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D009638:Norepinephrine