Modified determination of dihydrostreptomycin in kidney, muscle and milk by HPLC.
A method is presented for the determination of dihydrostreptomycin in milk, muscle and kidney by reversed-phase ion-pair high-performance liquid chromatography and post-column derivatisation with beta-naphthoquinone-4-sulfonate prior to fluorescence detection. The new sample work-up procedures include acid precipitation of proteins and, in the case of muscle and kidney, removal of fats by solvent extraction followed by solid phase extraction on a cation exchanger. The fluorescence response was linear from 25 to 2000 micrograms l-1 of injected analyte. The detection limits were 10 micrograms kg-1 for milk and 15 micrograms kg-1 for muscle and kidney and the analyte recoveries were on average 93% for milk, 70% for kidney and 75% for muscle.
Abbasi
H
H
National Food Administration, Uppsala, Sweden.
Hellenäs
K E
KE
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis* / Q000737:chemistry; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D019155:Veterinary Drugs / Q000032:analysis*
D000900:Anti-Bacterial Agents; D019155:Veterinary Drugs; D004096:Dihydrostreptomycin Sulfate