Synthesis and immunologic characterisation of Mycobacterium tuberculosis lipoarabinomannan specific oligosaccharide-protein conjugates.
Lipoarabinomannan (LAM) is a major structural surface component of all mycobacteria, and has been reported to have a wide range of biological effects. Immunogenic LAM specific oligosaccharide protein conjugates were synthesized and immunologically characterized. Oligosaccharides were derived from LAM purified from Mycobacterium tuberculosis H37 Rv and covalently conjugated to tetanus toxoid and cross reactive mutant (CRM197) diphtheria toxoid. Both types of LAM oligosaccharide protein conjugates proved to be highly immunogenic, inducing a boosterable T helper cell dependent IgG response. These conjugates are currently evaluated as components in a subcellular experimental TB vaccine.
Hamasur
B
B
Swedish Institute for Infectious Disease Control, Stockholm.
Källenius
G
G
Svenson
S B
SB
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology*; D009844:Oligosaccharides / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000276:immunology*; D011817:Rabbits; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000737:chemistry
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D018074:Vaccines, Conjugate; C050016:lipoarabinomannan