Cultured rat microglia express functional beta-chemokine receptors.
We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia.
Boddeke
E W
EW
Department of Medical Physiology, University of Groningen, The Netherlands. h.w.g.m.boddeke@med.rug.nl
Meigel
I
I
Frentzel
S
S
Gourmala
N G
NG
Harrison
J K
JK
Buttini
M
M
Spleiss
O
O
Gebicke-Härter
P
P
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000276:immunology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000494:pharmacology; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology; D017628:Microglia / Q000737:chemistry* / Q000166:cytology / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000235:genetics / Q000276:immunology; D019707:Receptors, Chemokine / Q000235:genetics* / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012433:Ryanodine / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
C515302:Ccr1 protein, rat; C515336:Ccr2 protein, rat; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D017931:DNA Primers; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; D012433:Ryanodine; D019284:Thapsigargin; D002118:Calcium