Characterization of a canine long-term T cell line (DLC 01) established from a dog with Sézary syndrome and producing retroviral particles.
The canine DLC 01 cell line derives from a lymph node of a dog with Sézary syndrome. The DLC 01 cell phenotype is CD4-, CD8+, CD45+, DQ+, similar to that of original cells after treatment with dimethylsulfoxide or phorbol myristate. Canine cutaneous T cell lymphoma are usually CD4-, CD8+ in contrast to their human counterparts which are CD4+, CD8-. Therefore, the DLC 01 cell line appears to be a unique model to study the mechanism of all surface molecule expression in vitro. Viral particles with retrovirus type-C morphology were found in ultrathin sections of DLC 01 cell pellets. Retroviral particles are spontaneously produced after the 50th cell passage or after induction with 0.5% dimethylsulfoxide. This is the first description of a dog lymphoid cell line spontaneously growing and producing a retrovirus. It was found to share several features in common with feline and murine leukemia viruses.
Ghernati
I
I
Establissement de Transfusion Sanguine, Gerland, France.
Auger
C
C
Chabanne
L
L
Corbin
A
A
Bonnefont
C
C
Magnol
J P
JP
Fournel
C
C
Rivoire
A
A
Monier
J C
JC
Rigal
D
D
D000818:Animals; D002415:Cats; D004283:Dog Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology; D004285:Dogs; D006801:Humans; D012190:Retroviridae / Q000302:isolation & purification; D012751:Sezary Syndrome / Q000276:immunology / Q000473:pathology / Q000662:veterinary / Q000821:virology; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology / Q000821:virology; D014407:Tumor Cells, Cultured