title: Determination of domperidone in human serum and human breast milk by high-performance liquid chromatography-electrospray mass spectrometry.
abstract: A sensitive and selective method for the determination of domperidone in human breast milk and serum has been developed. The same method may be successfully applied to both matrices to a lower limit of quantitation of 0.5 ng/ml. Samples are processed by a liquid-liquid extraction, and analyzed by LC-ESI-MS in positive ion mode. There was no interference, on the domperidone quantitation, from over 30 drugs. Samples from patients, at various times post-dose, were analyzed and a large number showed significant levels of domperidone in the breast milk as well as in the serum.
authors.affiliation: Hewlett-Packard (Canada) Ltd., Chemical Analysis Division, Mississauga, Ontario. paul_zavitsanos@hp.com
authors.forename: A P
authors.initials: AP
authors.lastname: Zavitsanos
authors.forename: C
authors.initials: C
authors.lastname: MacDonald
authors.forename: E
authors.initials: E
authors.lastname: Bassoo
authors.forename: D
authors.initials: D
authors.lastname: Gopaul
mesh_terms: D000932:Antiemetics / Q000032:analysis* / Q000097:blood; D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000032:analysis* / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008895:Milk, Human / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000932:Antiemetics; D004294:Domperidone