title: N-acylphosphatidylethanolamine-hydrolysing phospholipase D lacks the ability to transphosphatidylate.
abstract: The N-acylphosphatidylethanolamine-hydrolysing phospholipase D (NAPE-PLD) generates N-acylethanolamines, including N-arachidonoyl-ethanolamine (anandamide), that may be neuroprotective and analgesic. The properties of NAPE-PLD from rat heart and brain microsomes are investigated and compared to those of other PLDs. NAPE-PLD is inhibited by the fatty acid aminohydrolase inhibitor MAFP in high concentrations (> or = 100 microM) while PMSF in high concentrations (10 mM) tends to stabilise NAPE-PLD activity. Oleate inhibits NAPE-PLD but the enzyme is not affected by PIP2, alpha-synuclein or mastoparan. Furthermore, it is for the first time reported that NAPE-PLD is not capable of catalysing a transphosphatidylation reaction like most other known PLDs.
authors.affiliation: Department of Pharmacology, The Royal Danish School of Pharmacy, Copenhagen.
authors.forename: G
authors.initials: G
authors.lastname: Petersen
authors.forename: H S
authors.initials: HS
authors.lastname: Hansen
mesh_terms: D000818:Animals; D003902:Detergents; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D017830:Octoxynol; D010712:Phosphatidic Acids / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003902:Detergents; D004791:Enzyme Inhibitors; D010712:Phosphatidic Acids; D010714:Phosphatidylethanolamines; D017830:Octoxynol; D010739:Phospholipase D