Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.
Epidermal growth factor (EGF) and heparin-binding EGF-like growth factor (HB-EGF) exert their activities through the same cell surface receptor. In the present study, the role of EGF in cell kinetics of rat gastric epithelial cell line, RGM-1, in which HB-EGF is known to function in an autocrine fashion, was investigated. EGF stimulated the mucus glycoprotein synthesis which is characterized with cell maturation, but competed with HB-EGF for cell proliferation. By flow cytometric analysis, EGF inhibited G2/M transition by 48 h after stimulation and induced apoptosis manifested by hypodiploid DNA by 72 h after stimulation. Consistent with this, DNA ladder formation together with the histone-associated DNA fragments was found in EGF-treated cells. These results indicate that EGF and HB-EGF possess different functions in RGM-1 cells and that EGF acts as a mediator of both cell maturation and apoptosis in these cells.
Hayashida
Y
Y
First Department of Internal Medicine, Nagasaki University of Medicine, Nagasaki 852-8501, Japan.
Nakata
K
K
Ishikawa
H
H
Taniguchi
K
K
Migita
K
K
Nakao
K
K
Kato
Y
Y
Eguchi
K
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D066257:Heparin-binding EGF-like Growth Factor; D006657:Histones / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D011003:Ploidies; D051381:Rats; D017208:Rats, Wistar
D006023:Glycoproteins; C587885:Hbegf protein, rat; D066257:Heparin-binding EGF-like Growth Factor; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D004815:Epidermal Growth Factor; D005944:Glucosamine