Lectin histochemistry of goblet cell sugar residues in the gut of the chick embryo and of the newborn.
The anlage of duodenum, ileum and colon were removed from chick embryos of day 8-21 of incubation and from 1-day-old chicks. A battery of seven different horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, Con A, WGA, LTA and UEAI) was used to study the carbohydrate residues of the glycoconjugates in the goblet cells of the three parts of the intestine. The main results can be summarized as follows: differences in lectin binding were absent in the proximal and distal parts of the duodenum, ileum and colon. Lectin histochemistry showed differences among the three intestinal segments for the time of appearance of the oligosaccharides in the goblet mucus. In the colonic goblet cells of 1-day-old chicks, LTA and UEAI lectins showed two different types of linkage of alpha-L-fucose. This is the first demonstration of UEAI reactive sites in Gallus domesticus.
Bryk
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SG
Department of Anatomy, Histology and Forensic Medicine, University of Florence, Italy. gheri@dada.it
Sgambati
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Gheri Bryk
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D000818:Animals; D000831:Animals, Newborn; D002241:Carbohydrates / Q000032:analysis*; D002642:Chick Embryo; D002645:Chickens; D003106:Colon / Q000737:chemistry / Q000196:embryology / Q000648:ultrastructure; D004386:Duodenum / Q000737:chemistry / Q000196:embryology / Q000648:ultrastructure; D020397:Goblet Cells / Q000737:chemistry* / Q000648:ultrastructure; D007082:Ileum / Q000737:chemistry / Q000196:embryology / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000032:analysis*
D002241:Carbohydrates; D037102:Lectins