Amplifications of DNA primase 1 (PRIM1) in human osteosarcoma.
We studied the involvement of PRIM1 in osteosarcoma by differential display, Northern and Southern hybridization, as well as fluorescence in situ hybridization (FISH) on interphase nuclei. In total, 22 pediatric oncology specimens were tested. PRIM1 was found to be amplified in 41% of the samples. PRIM1 is coamplified with the core 12q13 amplicon genes CDK4, SAS, and OS9, and was physically mapped very close to them. PRIM1 is therefore a new candidate for the role of a major target gene of 12q13 amplifications in human cancers. Genes Chromosomes Cancer 26:62-69, 1999.
Yotov
W V
WV
Département de Pédiatrie, Hôpital Ste-Justine, Université de Montréal, Montréal, Québec, Canada. yotovw@ere.umontreal.ca
Hamel
H
H
Rivard
G E
GE
Champagne
M A
MA
Russo
P A
PA
Leclerc
J M
JM
Bernstein
M L
ML
Levy
E
E
D015152:Blotting, Northern; D001859:Bone Neoplasms / Q000201:enzymology / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D019915:DNA Primase / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D037102:Lectins; D008565:Membrane Proteins / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D012516:Osteosarcoma / Q000201:enzymology / Q000235:genetics*; D020161:Physical Chromosome Mapping; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000235:genetics; D059470:Tetraspanins; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D037102:Lectins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C098898:OS9 protein, human; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C086385:TSPAN31 protein, human; D059470:Tetraspanins; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D019915:DNA Primase