An inducible human immunodeficiency virus type 1 (HIV-1) vector which effectively suppresses HIV-1 replication.
Recently, gene therapy vectors based upon the human immunodeficiency virus type 1 (HIV-1) genome have been developed. Here, we create an HIV-1 vector which is defective for all HIV-1 genes, but which maintains cis-acting elements required for efficient packaging, infection, and expression. In T cells transduced by this vector, vector expression is low but efficiently induced following HIV-1 infection. Remarkably, although the HIV-1 vector does not contain specific anti-HIV-1 therapeutic genes, the presence of the vector alone is sufficient to inhibit the spread of HIV-1 infection. The mechanism of inhibition is likely to be at the level of competition for limiting substrates required for either efficient packaging or reverse transcription, thereby selecting against propagation of wild-type HIV-1. These results provide proof of a concept for potential application of a novel HIV-1 vector in HIV-1 disease.
An
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DS
Departments of Microbiology & Immunology and Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA.
Morizono
K
K
Li
Q X
QX
Mao
S H
SH
Lu
S
S
Chen
I S
IS
D002461:Cell Line, Transformed; D005822:Genetic Vectors / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D019065:Virus Assembly; D014779:Virus Replication