title: Chromosomal double-strand breaks introduced in mammalian cells by expression of I-Sce I endonuclease.
authors.affiliation: Molecular Biology Program, Sloan-Kettering Institute, New York, NY, USA.
authors.forename: C
authors.initials: C
authors.lastname: Richardson
authors.forename: B
authors.initials: B
authors.lastname: Elliott
authors.forename: M
authors.initials: M
authors.lastname: Jasin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008322:Mammals; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity; D014162:Transfection / Q000379:methods
chemical_list: D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C037725:SCEI protein, S cerevisiae; C052380:endodeoxyribonuclease NcoI; D015252:Deoxyribonucleases, Type II Site-Specific