Divergent effects of paracetamol on reactive oxygen intermediate and reactive nitrogen intermediate production by U937 cells.
We investigated the effects of paracetamol on reactive oxygen intermediate and reactive nitrogen intermediate production by U937 cells. Cells which had been treated with paracetamol (0.01 microM - 10 microM) exhibited a significant (p<0.001) increase in hydrogen peroxide production and a significant (p<0.001) increase in phorbol 12-myristate 13-acetate induced superoxide production. Conversely, paracetamol (0.01 microM - 10 microM) caused a significant (p<0.001) decrease in the amount of nitrite secreted into the culture medium. Our results indicate divergent effects of paracetamol on ROI and RNI production.
Jamil
I
I
Division of Biomedical Sciences, School of Health Sciences, University of Wolverhampton, Wolverhampton WV1 1DJ, UK.
Symonds
A
A
Lynch
S
S
Alalami
O
O
Smyth
M
M
Martin
J
J
D000082:Acetaminophen / Q000494:pharmacology*; D016227:Benzoquinones / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007097:Imines / Q000378:metabolism; D009000:Monocytes; D009573:Nitrites / Q000037:antagonists & inhibitors*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate; D020298:U937 Cells
D016227:Benzoquinones; D005609:Free Radicals; D007097:Imines; D009573:Nitrites; D017382:Reactive Oxygen Species; D013481:Superoxides; D000082:Acetaminophen; D006861:Hydrogen Peroxide; C028473:N-acetyl-4-benzoquinoneimine; D013755:Tetradecanoylphorbol Acetate