Developmental expression of the four plasma membrane calcium ATPase (Pmca) genes in the mouse.
The plasma membrane calcium ATPases are critical components in the regulation of cellular calcium homeostasis and signaling. In mammals, there are 4 Pmca genes, and information on the cellular and tissue distribution of their expression during development will provide insight into the regulation and possible function of each Pmca isoform. Using specific probes and in situ hybridization, we found that the four Pmca genes are expressed in spatially overlapping but distinct patterns in the mouse embryo. The dynamic temporal patterns of expression indicate that the individual isoforms are subject to both positive and negative regulation. The differential and restricted expression of Pmca genes supports the notion that they play unique functional roles in mammalian development.
Zacharias
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Department of Biochemistry and Molecular Biology, Samuel C. Johnson Medical Research Center, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA.
Kappen
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D000818:Animals; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D027682:Cation Transport Proteins; D002478:Cells, Cultured; D004396:Coloring Agents; D053938:DNA Fragmentation; D018076:DNA, Complementary / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D008099:Liver / Q000196:embryology; D008297:Male; D051379:Mice; D009420:Nervous System / Q000196:embryology; D053499:Plasma Membrane Calcium-Transporting ATPases; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors; D001743:Urinary Bladder / Q000196:embryology
D027682:Cation Transport Proteins; D004396:Coloring Agents; D018076:DNA, Complementary; D012333:RNA, Messenger; D053499:Plasma Membrane Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases