title: A 45-kDa cAMP-dependent phosphoprotein which is related to the product of Mst57Dc in Drosophila melanogaster.
abstract: In Drosophila, the catalytic subunit of cAMP-dependent protein kinase (PKA) is preferentially expressed in the brain and the male reproductive organs. Although the cAMP response element binding protein (CREB) is a major target of PKA in the brain, the target of PKA in the male reproductive organs has been unknown. In the present study, three cAMP-dependent phosphoproteins (referred to as pp45, pp20, and pp10) were detected in the lumen fluid of male accessory glands. They were tissue-specific secretory proteins that accumulated only after eclosion, and were transferred to females during mating as other secretory proteins of the accessory glands. Among them, the 45-kDa phosphoprotein was partially purified and characterized. The purified protein was phosphorylated in vitro by the catalytic subunit of PKA. The partial amino acid sequence of this 45-kDa phosphoprotein was identical to the predicted amino acid sequence of the Mst57Dc cDNA, which is a male accessory gland transcript.
authors.affiliation: Department of Molecular Biology, Seoul National University, South Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Cho
authors.forename: J H
authors.initials: JH
authors.lastname: Lim
authors.forename: D H
authors.initials: DH
authors.lastname: Won
authors.forename: M C
authors.initials: MC
authors.lastname: Gye
authors.forename: K W
authors.initials: KW
authors.lastname: Chung
authors.forename: C C
authors.initials: CC
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D018076:DNA, Complementary; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D012726:Sexual Behavior, Animal
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D010750:Phosphoproteins; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases