Effect of heat acclimation and heat shock on oscillations of carbamyl-choline-evoked Ca2+ signal in HSY cell line.
We studied effects of heat acclimation (HA) and acute heat stress (HS) on Ca2+ signal oscillations following supramaximal carbamyl-choline (CCh) stimulation, using HSY cell line as a model. In the control cells, oscillations decreased their amplitude with time. HS alone did not change either oscillation amplitude or frequency, although calcium release to the cytosol upon CCh stimulation was faster. HA increased maximal oscillation amplitude only. There was no change in basal cytosolic calcium level and peak evoked signal in all experimental conditions. Collectively, the data suggest that HA affects the oscillation profile. Changes in the oscillation profile did not correlate with changes in the resting and evoked Ca2+ signal, which suggests that the oscillations are a separate target for heat acclimation.
Kaspler
P
P
Department of Physiology, Hadassah School of Dental Medicine, Hebrew University, Jerusalem, Israel.
Moran
A
A
Horowitz
M
M
D000064:Acclimatization / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002217:Carbachol / Q000494:pharmacology*; D002460:Cell Line; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D018721:Muscarinic Agonists / Q000494:pharmacology*; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D018721:Muscarinic Agonists; D002217:Carbachol; D002118:Calcium