title: TGF-beta is elevated in the CSF of patients with severe traumatic brain injuries and parallels blood-brain barrier function.
abstract: Traumatic brain injury (TBI) induces local and systemic immunologic changes, release of cytokines, and cell activation. Perpetuation of these cascades may contribute to secondary damage to the brain. Therefore, the ability of the antiinflammatory mediator transforming growth factor-beta (TGF-beta) to downregulate intrathecal immunoactivation may be of fundamental value for diminishing the incidence and extent of secondary insults. In this study, the release of TGF-beta into cerebrospinal fluid (CSF) and serum of 22 patients with severe TBI was analyzed with respect to the function of the blood-brain barrier (BBB) for 21 days. Levels of TGF-beta in CSF increased to their maximum on the first day (median, 1.26 ng/mL), thereafter decreasing gradually over time. Median TGF-beta values in serum always remained within the reference interval (6.5 to 71.5 ng/mL). Daily assessment of the CSF-serum albumin quotient (QA) and of the CSF-serum TGF-beta quotient (QTGF-beta) showed a strong correlation between maximal QTGF-beta and QA, indicating a passage of this cytokine from the periphery to the intrathecal compartment across the BBB. However, calculation of the TGF-beta index (QTGF-beta/Q(A)) suggested a cerebral production of TGF-beta in 9 of 22 patients. Levels of TGF-beta could not be correlated with extent of initial injury by computed tomography (CT), CD4/CD8 ratios, acute lung injury, or clinical outcome as rated by the Glasgow Outcome Scale (GOS). Although increased levels of TGF-beta in CSF seem to parallel BBB function, a partial intrathecal production is suggested, possibly modulated by elevation of interleukin-6 (IL-6). Thus, TGF-beta may function as a factor in the complex cytokine network following TBI, acting as an antiinflammatory and neuroprotective mediator.
authors.affiliation: Department of Surgery, University Hospital, Zurich, Switzerland. cristina.kossmann@chi.usz.ch
authors.forename: M C
authors.initials: MC
authors.lastname: Morganti-Kossmann
authors.forename: V H
authors.initials: VH
authors.lastname: Hans
authors.forename: P M
authors.initials: PM
authors.lastname: Lenzlinger
authors.forename: R
authors.initials: R
authors.lastname: Dubs
authors.forename: E
authors.initials: E
authors.lastname: Ludwig
authors.forename: O
authors.initials: O
authors.lastname: Trentz
authors.forename: T
authors.initials: T
authors.lastname: Kossmann
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001812:Blood-Brain Barrier / Q000502:physiology*; D001930:Brain Injuries / Q000134:cerebrospinal fluid* / Q000473:pathology / Q000503:physiopathology; D016207:Cytokines / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D015600:Glasgow Coma Scale; D006801:Humans; D007278:Injections, Spinal; D015850:Interleukin-6 / Q000097:blood / Q000134:cerebrospinal fluid; D007700:Kinetics; D008168:Lung / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets; D014057:Tomography, X-Ray Computed; D016212:Transforming Growth Factor beta / Q000097:blood / Q000134:cerebrospinal fluid*
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016212:Transforming Growth Factor beta