The effect of takusha, a kampo medicine, on renal stone formation and osteopontin expression in a rat urolithiasis model.
Kampo medicine is a traditional Japanese therapeutic system which originated in China and was used to treat various diseases for hundreds of years. Kampo medicine had been also used for the cure and the prevention of urinary calculi for many years, but the effect and the mechanism of this use of kampo medicine are unclear. We examined the inhibitory effect of the kampo medicine takusha on the formation of calcium oxalate renal stones induced by ethylene glycol (EG) and vitamin D3 in rats. We also investigated the effect of takusha on osteopontin (OPN) expression, which we previously identified as an important stone matrix protein. The control group rats were non-treated; the stone group rats were administered EG and vitamin D3, and the takusha group was administered takusha in addition to EG and vitamin D3. The rate of renal stone formation was lower in the takusha group than in the stone group; thus, the OPN expression in the takusha group was smaller than in the stone group. Takusha was effective in preventing oxalate calculi formation and OPN expression in rats. These findings suggest that takusha prevents stone formation including not only calcium oxalate aggregation but also proliferation.
Yasui
T
T
Department of Urology, Nagoya City University Medical School, Nagoya, Japan. yasui@med.nagoya-cu.ac.jp
Fujita
K
K
Sato
M
M
Sugimoto
M
M
Iguchi
M
M
Nomura
S
S
Kohri
K
K
D000818:Animals; D002129:Calcium Oxalate / Q000378:metabolism; D002762:Cholecalciferol / Q000008:administration & dosage / Q000633:toxicity; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019855:Ethylene Glycol / Q000008:administration & dosage / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects; D007669:Kidney Calculi / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D053495:Osteopontin; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D002762:Cholecalciferol; D002129:Calcium Oxalate; D019855:Ethylene Glycol