title: Determination of polychlorinated biphenyls in human blood serum by SPME.
abstract: We report a direct immersion SPME-GC/mu ECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9% to 3.4% (n = 10) was obtained. Analyte recovery up to 93% and detection limit of 1.0 ppb (total PCB) were achieved.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Kowloon.
authors.forename: K F
authors.initials: KF
authors.lastname: Poon
authors.forename: P K
authors.initials: PK
authors.lastname: Lam
authors.forename: M H
authors.initials: MH
authors.lastname: Lam
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000378:metabolism; D020111:Chlorodiphenyl (54% Chlorine) / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D019286:Endopeptidase K; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D001798:Blood Proteins; D010446:Peptide Fragments; D020111:Chlorodiphenyl (54% Chlorine); D011078:Polychlorinated Biphenyls; D019286:Endopeptidase K