title: Evaluation of laser doppler flowmetry for the study of benign and malignant gastric blood flow in vivo.
abstract: BACKGROUND
  Tumour vascularisation is a determinant of the development of metastases.
  
  
  OBJECTIVE
  To measure blood flow in normal stomach and gastric adenocarcinomas by laser Doppler flowmetry and correlate blood flow with vascularisation after immunohistochemical staining of resected specimens for CD31 and von Willebrand factor.
  
  
  METHODS
  Twenty two undergoing resection for gastric adenocarcinoma and 10 undergoing cholecystectomy.
  
  
  RESULTS
  Mean (SD) gastric blood flow was 208 (35) perfusion units (PU) in patients undergoing cholecystectomy and 190 (75) PU in the undiseased part of the stomach in patients with gastric adenocarcinoma. Gastric blood flow was higher in the border of gastric adenocarcinomas (322 (120) PU, p<0.01 v normal stomach) but lower in the centre (74 (27) PU, p<0.01 v normal stomach and tumour border). Blood flow was higher in tumours staged T>/=3 than in those staged T<3. Blood vessel density in normal stomach was 41 (8) stained cells/field viewed and was 1. 9-3.4 times higher in gastric adenocarcinomas.
  
  
  CONCLUSIONS
  Laser Doppler flowmetry is a valuable tool for studying the pathophysiological alterations of malignant blood flow in the human stomach in vivo.
authors.affiliation: Department of Visceral and Transplant Surgery, University of Bern, Inselspital, Bern, Switzerland.
authors.forename: M K
authors.initials: MK
authors.lastname: Schilling
authors.forename: C
authors.initials: C
authors.lastname: Redaelli
authors.forename: H
authors.initials: H
authors.lastname: Friess
authors.forename: B
authors.initials: B
authors.lastname: Blum
authors.forename: C
authors.initials: C
authors.lastname: Signer
authors.forename: C A
authors.initials: CA
authors.lastname: Maurer
authors.forename: M W
authors.initials: MW
authors.lastname: Büchler
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017078:Laser-Doppler Flowmetry; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012039:Regional Blood Flow; D013270:Stomach / Q000098:blood supply*; D013274:Stomach Neoplasms / Q000098:blood supply*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor