[Sex determination of human preimplantation embryo blastomere using dualcolor fluorescence in situ hybridization].
OBJECTIVE
To establish a series of techniques of sex determination of human preimplantation embryo in order that preimplantation genetic diagnosis(PGD) can be used clinically.


METHODS
Use micromanipulation for biopsy of human preimplantation embryo(3-10 cell) and prepare single-cell interphase nuclei sample. Make sex determination of human preimplantation embryos by using dual-color fluorescence in situ hybridization.


RESULTS
The technique of human preimplantation embryo (3-10 cell) biopsy was established preliminarily and the technique of preparing single-cell interphase nuclei sample was set up and stabilized. The sex determination of the blastomere of human preimplantation embryo was successful.


CONCLUSIONS
This series of techniques can make fast diagnosis (less than 6 hours) with minimal sample(only one cell), with no cell culture, and with high accuracy of sex determination (100%).
Li
W
W
Human Reproductive Engineering Laboratory, Hunan Medical University, Changsha, 410078 P.R.China. threlhmu@public.cs.hn.cn
Lu
G
G
D001755:Blastocyst; D001757:Blastomeres; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008846:Micromanipulation; D012732:Sex Determination Analysis