[Genetic polymorphisms of five STR loci in a Chinese Han population].
OBJECTIVE
To understand the allele structure and genetic polymorphism at five STR loci in Chinese Han population, and construct a preliminary database.


METHODS
EDTA-blood specimens were collected from the unrelated individuals. The DNA samples were extracted with Chelex method and were amplified by PCR technique. The PCR products were analyzed using both the PAGE horizontal electrophoresis with discontinuous buffer system and the automated fluorescence detection approach.


RESULTS
Four STRs consist of simple repeat motifs, while one STR contains a complex repeat structure. The STR polymorphisms at all of the five loci have been observed in Chinese Han population.


CONCLUSIONS
The obtained data are beneficial to understanding the population genetics of the five STR loci in Chinese Han population. As a simple approach, the PAGE horizontal electrophoresis can be employed for typing the five STR markers.
Hou
Y
Y
Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, 610041 P.R.China. rechtsme@wcums.edu.cn
Wu
J
J
Li
Y
Y
Tang
J
J
Zhang
S
S
Chu
J
J
Prinz
M
M
D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D005828:Genetics, Population; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences