DNA-induced conformational change of Gaf1, a novel GATA factor in Schizosaccharomyces pombe.
A novel GATA factor in Schizosaccharomyces pombe, Gaf1, containing one zinc-finger motif was studied for conformational change that was induced by DNA-binding. Gaf1 was shown to bind to the upstream activation sequence of a gene in Saccharomyces cerevisiae containing GATA element by gel mobility shift assay. Circular dichroism spectra of Gaf1 indicated an increase of alpha-helix content of Gaf1 occurred upon binding to the upstream activation sequence. These results suggest that the binding of Gaf1 to the GATA element is required for the conformational change that may precede transactivation of the target gene(s).
Won
M
M
Cell Cycle and Signal Transduction Research Unit, Korea Research Institute of Bioscience & Biotechnology, Taejon.
Hoe
K L
KL
Cho
Y S
YS
Song
K B
KB
Yoo
H S
HS
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D016384:Consensus Sequence; D004247:DNA / Q000378:metabolism*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D016335:Zinc Fingers
C114485:Gaf1 protein, S pombe; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators; D004247:DNA