title: Lymphatic absorption of structured triglycerides vs. physical mix in a rat model of fat malabsorption.
abstract: Comparison was made between the intestinal absorption and lymphatic transport of a randomly interesterified fish oil and medium-chain triglyceride (MCT) structured triglycerides (STG) vs. the physical mix in rat small intestine following ischemia and reperfusion (I/R) injury. Under halothane anesthesia, the superior mesenteric artery (SMA) was occluded for 20 min and then reperfused in I/R rats. The SMA was isolated but not occluded in control rats. In both treatment groups, the mesenteric lymph duct was cannulated and a gastric tube was inserted. Each treatment group received 1 ml of the fish oil-MCT STG or physical mix (7 rats/group) through the gastric tube followed by an infusion of PBS at 3 ml/h for 8 h. Lymph was collected hourly for 8 h. Lymph triglyceride, cholesterol, and decanoic and eicosapentaenoic acids increased rapidly and maintained a significantly higher output (P < 0.01) with STG compared with physical mix in control rats over 8 h. After I/R, lymphatic triglyceride output decreased 50% compared with control. Gastric infusion of STG significantly improved lipid transport by having a twofold higher triglyceride, cholesterol, and decanoic and eicosapentaenoic acids output to lymph compared with its physical mix (P < 0.01). We conclude that STG is absorbed into lymph significantly better than physical mix by both the normal intestine and the intestine injured by I/R.
authors.affiliation: Department of Pathology, University of Cincinnati Medical Center, Cincinnati 45267, Ohio, USA. tsopp@email.uc.edu
authors.forename: P
authors.initials: P
authors.lastname: Tso
authors.forename: T
authors.initials: T
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Demichele
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005223:Fats / Q000493:pharmacokinetics*; D005395:Fish Oils / Q000493:pharmacokinetics; D008196:Lymph / Q000378:metabolism / Q000502:physiology; D008208:Lymphatic System / Q000378:metabolism*; D008286:Malabsorption Syndromes / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005223:Fats; D005395:Fish Oils; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D015118:Eicosapentaenoic Acid