title: Influence of disulfide bonds on the induction of helical conformation in proteins.
abstract: The effect(s) of TFE (2,2,2-trifluoroethanol) on three different conformational states (native, denatured, and carboxymethylated) of CTX III and RNase A has been examined. Contrary to the general belief, the results of the present study reveal that TFE can induce helical conformation in a protein which has no sequence propensity to form a helix. It is found that the helix induction in TFE is intricately related to the destabilization of the tertiary structural conformation in proteins. More importantly, the disulfide bonds in proteins are found to have significant influence on the TFE-mediated helix induction. The results obtained in this study strongly suggest that information pertaining to the influence of disulfide bonds on helix induction need to be considered to improve the accuracy of secondary structure prediction algorithms.
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: T
authors.initials: T
authors.lastname: Sivaraman
authors.forename: T K
authors.initials: TK
authors.lastname: Kumar
authors.forename: K W
authors.initials: KW
authors.lastname: Hung
authors.forename: C
authors.initials: C
authors.lastname: Yu
mesh_terms: D002942:Circular Dichroism; D004179:Cobra Cardiotoxin Proteins / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation / Q000187:drug effects*; D011489:Protein Denaturation; D017433:Protein Structure, Secondary / Q000187:drug effects*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D014270:Trifluoroethanol / Q000494:pharmacology*
chemical_list: D004179:Cobra Cardiotoxin Proteins; D004220:Disulfides; D014270:Trifluoroethanol; D012259:Ribonuclease, Pancreatic