The accentuating effect of low magnesium concentration on cholestane-3 beta, 5 alpha, 6 beta-triol-induced decrease of LDL uptake by cultured endothelial cells.
The effects of cholestane-3 beta, 5 alpha, 6 beta-triol (TriolC) and/or low magnesium on uptake, internalization, surface binding and degradation of 125I-LDL were investigated in cultured endothelial cells. TriolC (at a level of 1 microgram/ml and higher) or magnesium (at a level of 188 microM and lower) in cultured medium decreased significantly the uptake, internalization, surface binding and degradation of 125I-LDL. When TriolC and low magnesium were combined, the significant inhibitions of uptake, internalization, surface binding and degradation of 125I-LDL were also observed although by themselves at these levels (TriolC lower than 1 microgram/ml and magnesium higher than 188 microM) they had no obvious effects. Our work suggests that a combination of low magnesium and TriolC have additive inhibitive effects on uptake, internalization, surface binding and degradation of 125I-LDL.
Zhou
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Harlan E. Moore Heart Research Foundation, Champaign, IL 61820, USA.
Hulea
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Kummerow
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D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002777:Cholestanols / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000960:Hypolipidemic Agents / Q000378:metabolism / Q000494:pharmacology*; D007457:Iodine Radioisotopes / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism / Q000493:pharmacokinetics*; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology
D002777:Cholestanols; D000960:Hypolipidemic Agents; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; C000474:cholestane-3,5,6-triol; D008274:Magnesium