Exceptional characteristics of heterotetrameric (alpha 2 beta 2) E1p of the pyruvate dehydrogenase complex from Zymomonas mobilis: expression from an own promoter and a lipoyl domain in E1 beta.
In the pyruvate dehydrogenase complex (PDHC) of Zymomonas mobilis the beta subunit of the pyruvate dehydrogenase (E1p) as well as the acetyltransferase (E2p) contain an N-terminal lipoyl domain. Both lipoyl domains were acetylated in vitro using 2-14C-pyruvate as a substrate, demonstrating that both lipoyl domains can accept acetyl groups from the E1 component. As previously shown the structural genes (pdhA alpha beta, pdhB, lpd) encoding the pyruvate dehydrogenase complex of Z. mobilis are located in two distinct gene clusters, pdhA alpha beta and pdhB-orf2-lpd (U. Neveling et al. (1998) J. Bacteriol. 180, 1540-1548). Analysis of pdh gene expression using lacZ fusions revealed that the DNA fragments upstream of pdhA alpha, pdhB and lpd each have promoter activities. These pdh promoter activities were 7-30-fold higher in Z. mobilis than in Escherichia coli.
Neveling
U
U
Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, Germany.
Bringer-Meyer
S
S
Sahm
H
H
D007700:Kinetics; D046911:Macromolecular Substances; D011401:Promoter Regions, Genetic; D017433:Protein Structure, Secondary; D030481:Pyruvate Dehydrogenase (Lipoamide); D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology* / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
D046911:Macromolecular Substances; D011768:Pyruvate Dehydrogenase Complex; D011993:Recombinant Fusion Proteins; D030481:Pyruvate Dehydrogenase (Lipoamide); C111305:pyruvate dehydrogenase E1 beta subunit; C065640:pyruvate dehydrogenase E1alpha subunit; D001616:beta-Galactosidase