[A simple method for determining antiepileptic preparations in biological fluids by using high-pressure liquid chromatography (HPLC)].
The authors suggest a simple HPLC method with ultraviolet detection at 200 nm for finding barbiturates, diphenine, etosuccimide, and carbamazepin in plasma and saline using an original technology of liquid-liquid extraction. It allows clear chromatograms with a minimal amount of interfering compounds, a high extraction percentage (90-100%) and sufficient sensitivity to be obtained. A method has also been worked out for detecting clonazepam on the basis of solid-phase extraction (cartridge "Diapak-C16") precolumn concentration, and ultraviolet detection at 200 and 215 nm. The suggested extraction technologies also make possible successful monitoring of antiepileptics in the saline, for which the plasma/saline coefficients are calculated and the dependence between the degree of phenobarbital extraction into the saline and its pH is demonstrated.
Dutov
A A
AA
Regional Medical Diagnostic Center, Chita, Russia.
Temnikova
I V
IV
Fedotova
A A
AA
Gol'tvanitsa
G A
GA
D000927:Anticonvulsants / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012463:Saliva / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000927:Anticonvulsants
Prostoĭ metod opredeleniia protivoépilepticheskikh preparatov v biologicheskikh zhidkostiakh s pomoshch'iu vysokoéffektivnoĭ zhidkostnoĭ khromatografii (VEZhKh).