Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy.
We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.
de Felipe
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Departamento de Bioquímica y Biología Molecular, Universidad Autónoma de Madrid, Facultad de Ciencias, Spain.
Martín
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Cortés
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Ryan
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Izquierdo
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D016475:3T3 Cells; D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D005537:Aphthovirus / Q000235:genetics*; D015153:Blotting, Western; D002678:Chimera; D015774:Ganciclovir / Q000494:pharmacology; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D010957:Plasmids; D013937:Thymidine Kinase / Q000235:genetics; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D000123:Acetyltransferases; C045336:puromycin N-acetyltransferase; D013937:Thymidine Kinase; D015774:Ganciclovir