title: Gas chromatographic determination of galactose in milk. Example of a switching valve used for the protection of the capillary column.
abstract: Galactose, a marker of heat treatment, has been analysed in milk as pentafluorobenzyloxime acetate by gas chromatography with flame ionization detection using a simple switching valve system. The procedure did not entail any prederivatization clean-up for lactose elimination from the sample. In a short pre-column, reagent and lactose derivative excess were separated and the galactose and internal standard derivatives were transferred to the analytical column by a four-port valve. Thus, the analytical column was protected from overloading, so avoiding rapid deterioration.
authors.affiliation: Istituto di Fisiologia Veterinaria e Biochimica, Milan, Italy.
authors.forename: L M
authors.initials: LM
authors.lastname: Chiesa
authors.forename: L
authors.initials: L
authors.lastname: Radice
authors.forename: R
authors.initials: R
authors.lastname: Belloli
authors.forename: P
authors.initials: P
authors.lastname: Renon
authors.forename: P A
authors.initials: PA
authors.lastname: Biondi
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005690:Galactose / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D005690:Galactose