Effects of cyclosporin A on contractile activity and cytoskeleton in chick embryo cardiomyocytes.
The effects of cyclosporin A (CsA), a clinically used immunosupressive drug, on contractile activity of chick cardiomyocytes grown as small aggregates or explants suspended on a network of elastic glass fibres or cultured in a monolayer were analysed in vitro with computer-aided image cytometry methods. At therapeutic concentrations (200-1500 ng/mL), CsA induced changes in the frequency and amplitude of the beating activity of cardiomyocytes 15 min after application. Longer treatment of cardiomyocytes, for 20-24 h, additionally induced changes in their shape and cytoskeleton organization (F-actin and alpha-actinin distribution). These results indicate that CsA is able to affect directly the contractile activity, morphology, and cytoskeleton architecture of heart cells.
Kołcz
J
J
Department of Cardiac and Vascular Surgery, Institute of Cardiology, Jagiellonian University, Kraków, Poland.
Drukała
J
J
Jurkiewicz
A
A
Pfitzner
R
R
Garlicki
M
M
Czyź
J
J
Korohoda
W
W
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D016572:Cyclosporine / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D006321:Heart / Q000187:drug effects* / Q000196:embryology / Q000502:physiology; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology*
D016572:Cyclosporine