Comparison of bone marrow extracellular matrices.
We have compared the structure and composition of adult and fetal bovine bone marrow extracellular matrices. In contrast to fetal bone marrow, adult bone marrow has more oval fenestration and accumulation of adipocytes as well as lower protein content. These differences could be due to remodeling of bone marrow tissue as it develops. Zymogram analysis of matrix metalloproteinase (MMP) and tissue inhibitor of MMP (TIMP) activities showed that fetal, but not adult bone marrow extract contained a 96-kDa MMP and TIMP-1 and -2. These activities may contribute to the structural differences between adult and fetal bone marrow tissues.
Lee
M
M
Department of Biological Sciences, Illinois State University, Normal, IL, 61790-4120, USA. mlee@molbio.princeton.edu
Christopherson
I P
IP
Lehman
J M
JM
Bennett
C J
CJ
Cheung
H T
HT
D000367:Age Factors; D000818:Animals; D001853:Bone Marrow / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005314:Embryonic and Fetal Development; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure*; D005629:Frozen Sections; D008666:Metalloendopeptidases / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D014020:Tissue Extracts; D019714:Tissue Inhibitor of Metalloproteinases / Q000032:analysis
D014020:Tissue Extracts; D019714:Tissue Inhibitor of Metalloproteinases; D008666:Metalloendopeptidases