title: Population distributions of allele frequency of apolipoprotein E by age and gender in Han Chinese.
abstract: OBJECTIVE
  To study apolipoproteins E (ApoE) allele frequency in Han Chinese based on age and gender from Shanghai metropolitan area.
  
  
  METHODS
  Healthy Han Chinese people (F: 237 and M: 412) were involved in this study. ApoE gene was amplified by PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCT GAG GCC GCG CT-3'. The PCR product was digested directly with 5 units of CfoI and separated by a 20% polyacrylamide nondenaturing gel.
  
  
  RESULTS
  ApoE*3 was the commonest allele which accounted for 86.4% of the isoforms, and ApoE*2 and ApoE*4 accounted for 6.2% and 7.5%, respectively. The allele and genotype frequencies were in Hardy-Weinberg equilibrium by comparison with that of the corresponding theoretical distribution (P > 0.05).
  
  
  CONCLUSIONS
  The frequencies of ApoE*2, ApoE*3, and ApoE*4 were demonstrated in the normal Chinese population.
authors.affiliation: Section of Human Genome Project, Shanghai Research Center of Life Sciences, Chinese Academy of Sciences, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Zhang
authors.forename: X Z
authors.initials: XZ
authors.lastname: Dong
authors.forename: W J
authors.initials: WJ
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.forename: L
authors.initials: L
authors.lastname: He
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001057:Apolipoproteins E / Q000235:genetics*; D044466:Asian People; D002681:China; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012737:Sex Factors
chemical_list: D001057:Apolipoproteins E