Enantiomeric separation of amino acids derivatized with fluoresceine isothiocyanate isomer I by micellar electrokinetic chromatography using beta- and gamma-cyclodextrins as chiral selectors.
Enantiomeric separation of 21 amino acids derivatized with fluoresceine isothiocyanate isomer I (FITC) has been studied by micellar electrokinetic chromatography using beta- and gamma-cyclodextrin (CD) as chiral selectors. Chiral resolution of 21 FITC derivatives of amino acids was achieved with both beta- and gamma-CD in 100 mM borate buffer (pH 9.5) containing 30 mM sodium dodecyl sulfate (SDS). The effects of CD concentration, SDS concentration and organic modifiers' concentration as well as capillary length were investigated. Chiral recognition capability of beta- and gamma-CD was compared. Gamma-CD was found to be a better chiral selector than beta-CD in terms of chiral resolution capability for FITC-amino acids.
Jin
L J
LJ
Department of Chemistry, National University of Singapore, Kent Ridge, Republic of Singapore.
Rodriguez
I
I
Li
S F
SF
D019840:2-Propanol / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology*; D007536:Isomerism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013997:Time Factors; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D000097:Acetonitriles; D000596:Amino Acids; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D012967:Sodium Dodecyl Sulfate; D016650:Fluorescein-5-isothiocyanate; C031215:betadex; C023792:gamma-cyclodextrin; D019840:2-Propanol; C032159:acetonitrile