An improved histochemical method for measuring nitric oxide synthase activity.
The previous quantitative histochemical method for measuring nitric oxide synthase (NOS) activity in tissue sections involved the loss of about 15 per cent of the NOS, presumably from the section into the reaction medium. Two changes are now described. The first is concerned with the preparation in the laboratory of the active reagent, lead ammonium citrate/acetate (LACA). The second change involves an improvement of the procedure for measuring NOS activity. The new method appears to retain all the measurable NOS activity inside the section.
Alaghband-Zadeh
J
J
Endocrine Laboratory, Department of Clinical Chemistry, Imperial College of Science, Technology & Medicine (Charing Cross Campus), London, UK.
Mehdizadeh
S
S
O'Farrell
A
A
Weisz
J
J
Bitensky
L
L
Chayen
J
J
D000085:Acetates; D000818:Animals; D019343:Citric Acid; D005260:Female; D006652:Histological Techniques; D007854:Lead; D019001:Nitric Oxide Synthase / Q000032:analysis*; D010087:Oxides; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D014021:Tissue Preservation
D000085:Acetates; D010087:Oxides; D019343:Citric Acid; D007854:Lead; C047365:lead oxide; D019001:Nitric Oxide Synthase