[The expression of c-fos gene in cultured bovine trabecular cells induced by epidermal growth factor].
OBJECTIVE
To investigate the effect of epidermal growth factor (EGF) on cultured trabecular cells.


METHODS
Molecular hybridization technique was used to investigate the effect of EGF on c-fos expression and its induction of bovine trabecular cells in vitro, and 3H-TdR incorporation method was applied to study the synthesis of DNA.


RESULTS
EGF enhanced the incorporation of 3H-TdR into trabecular cells at concentrations ranging from 20-150 ng/ml (P < 0.001). Compared with control, the expression of c-fos appeared at 0.5 hour and 1 hour after stimulation, and did not exist till 2 hr. The level of c-fos expression in cells relied on the concentration of EGF.


CONCLUSIONS
Our results suggest that EGF stimulate trabecular cell proliferation at least in part by the regulation of the c-fos gene and its nuclear protein.
Yang
X
X
Department of Ophthalmology, First Hospital, Beijing Medical University.
Li
M
M
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*
D016760:Proto-Oncogene Proteins c-fos; D004815:Epidermal Growth Factor