title: The presence of traces of iron and copper ions during gamma-irradiation does not result in clear mutational hot spots in the lacI gene.
abstract: Oxidative radicals, which are produced during ionizing irradiation of DNA in water, damage the DNA and may result in mutations, which are in general randomly distributed. Alternatively, the addition of transition metal ions, like iron or copper, to DNA in combination with H(2)O(2) and a reducing agent also results in the production of oxidative radicals. Due to binding of the transition metal ions to DNA, the production of these radicals is very local, and results in a mutational spectrum in which the mutations are not randomly distributed. If transition metal ions are complexed to the DNA during irradiation, and react with radiation-induced species such as hydrogen peroxide, site-specific formation of.OH radicals on these sites may occur, leading to the formation of mutational hot spots. This study examines the influence of the presence of traces of iron or copper ions during gamma-irradiation of plasmid DNA in water, on the possible formation of mutational hot spots in the lacI gene. Comparison of the mutational spectra, after irradiation in the presence or in the absence of transition metal ions, shows that there are indeed relatively more positions in the lacI gene where more than one mutation occurs, suggesting formation of mutational hot spots in the presence of transition metal ions. However, the appearance of these hot spots is rather weak. Although in all three mutational spectra G:C to A:T mutations are predominant, there are also some differences between the types of mutations in these spectra. These differences in mutational spectra might reflect the different preferences of iron and copper ions to bind specific sites in the DNA. Indeed, there appears to be a high association of mutations at CC or GG sites in the mutational spectrum in the presence of copper ions, confirming the observation that copper binds preferably at two adjacent guanines in the DNA. It can be concluded from this study that the presence of small amounts of transition metal ions during gamma-irradiation influences the types and distribution of gamma-radiation-induced mutations, although no major mutational hot spots can be observed.
authors.affiliation: Department of Radiotherapy, Section Radiobiology, Faculty of Medicine, Vrije Universiteit, van der Boechorststraat 7, 1081 BT, Amsterdam, The, Netherlands. ca.wijker.oncol@med.vu.nl
authors.forename: C A
authors.initials: CA
authors.lastname: Wijker
authors.forename: M V
authors.initials: MV
authors.lastname: Lafleur
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D020029:Base Pairing / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D003300:Copper / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D029968:Escherichia coli Proteins; D005296:Ferrous Compounds / Q000494:pharmacology; D005720:Gamma Rays; D007501:Iron / Q000494:pharmacology*; D056968:Lac Repressors; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D010957:Plasmids / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D012097:Repressor Proteins / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects / Q000528:radiation effects
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005296:Ferrous Compounds; D056968:Lac Repressors; C539064:LacI protein, E coli; D012097:Repressor Proteins; C020748:ferrous sulfate; D003300:Copper; C028419:cuprous chloride; D007501:Iron