Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system.
Recently we cloned 140 kDa form of feline CD56 cDNA. In this study, we expressed the feline CD56 molecule by the baculovirus expression system. We found that the molecule was expressed on the cell surface when examined by the indirect immunofluorescence assay using an anti-human CD56 monoclonal antibody. Immunoblotting analysis revealed that the molecular weight of the major expressed product was 140 kDa. Interestingly we found that the insect cells expressing the feline CD56 molecule aggregated, indicating that the expressed molecule mediates homophilic adhesion.
Nishimura
Y
Y
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Miyazawa
T
T
Ikeda
Y
Y
Izumiya
Y
Y
Nakamura
K
K
Cai
J S
JS
Sato
E
E
Kohmoto
M
M
Mikami
T
T
Takahashi
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D016367:Baculoviridae; D019002:CD56 Antigen / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002415:Cats; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D007313:Insecta / Q000166:cytology
D000911:Antibodies, Monoclonal; D019002:CD56 Antigen; D018076:DNA, Complementary