Identification of a herpes simplex virus-induced dendrite in an eye-bank donor cornea.
OBJECTIVE
To demonstrate the presence of an active herpetic dendrite in an eye-bank cornea.


METHODS



METHODS
One eye-bank cornea was studied. Viral cultures and polymerase chain reaction (PCR) testing were performed 4 days after tissue preservation. The presence or absence of herpes simplex virus (HSV) type 1 was investigated.


RESULTS
The presence of an active HSV dendrite in an eye-bank cornea was verified. HSV type 1 was confirmed using PCR amplification and restriction endonuclease DNA fragment analysis.


CONCLUSIONS
This case suggests that HSV may remain viable in stored corneal tissue at 4 degrees C.
Neufeld
M V
MV
Department of Ophthalmology, University of Arkansas for Medical Sciences, Little Rock 72205-7199, USA.
Steinemann
T L
TL
Merin
L M
LM
Stroop
W G
WG
Brown
M F
MF
D003315:Cornea / Q000473:pathology* / Q000821:virology; D016039:Corneal Transplantation; D053938:DNA Fragmentation; D004279:DNA, Viral / Q000032:analysis; D005125:Eye Banks; D005500:Follow-Up Studies; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007635:Keratitis, Dendritic / Q000175:diagnosis* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014019:Tissue Donors
D004279:DNA, Viral