title: Dephosphorylation of specific proteins during induction of senescence in immortal human fibroblasts expressing thermolabile SV40 T antigen.
abstract: Particular protein kinase inhibitors block a senescence-like phenomenon in SVts8 cells induced by a shift up in temperature. We characterized cellular proteins with affinity chromatography using one such inhibitor as a ligand. Two proteins of 56 and 100 kDa were found to be dephosphorylated specifically, probably due to induction of a protein phosphatase activity(s).
authors.affiliation: Division of Biochemistry, Kihara Institute for Biological Research, Yokohama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujii
authors.forename: A
authors.initials: A
authors.lastname: Joguchi
authors.forename: H
authors.initials: H
authors.lastname: Ogino
authors.forename: K
authors.initials: K
authors.lastname: Nakabayashi
authors.forename: D
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D000375:Aging / Q000502:physiology*; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts; D006358:Hot Temperature; D006801:Humans; D008024:Ligands; D008970:Molecular Weight; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004791:Enzyme Inhibitors; D008024:Ligands; D047428:Protein Kinase Inhibitors; D011506:Proteins