Antibodies to the truncated (short) form of 'O' polysaccharides (TFOP) of Vibrio cholerae O139 lipopolysaccharides protect mice against experimental cholera induced by encapsulated O139 strains and such protection is mediated by inhibition of intestinal colonization of vibrios.
An antiserum raised against the lipopolysaccharides (LPS) of an encapsulated Vibrio cholerae O139 strain was shown to induce passive protection against challenge with O139, but not O1, organisms. Subsequent experiments, however, revealed that the purified LPS, obtained by the conventional phenol-water extraction method, contained capsular polysaccharide (CPS) material. Therefore, another antiserum was raised by immunization with electrophoresed gel-cut material containing only the truncated (short) form of 'O' polysaccharides (TFOP) linked to the core of O139 LPS. Anti-TFOP antibodies and their Fab (IgG) fragments induced passive protection against challenge with colonial variants of encapsulated O139 strains and such protection was mediated by inhibition of intestinal colonization. These results suggest that it is possible to engender protection against encapsulated O139 strains by using TFOP material (devoid of CPS) as the immunogen.
Nandy
R K
RK
Department of Microbiology, Bose Institute, Calcutta, India.
Mukhopadhyay
S
S
Ghosh
A N
AN
Ghose
A C
AC
D000818:Animals; D002771:Cholera / Q000276:immunology / Q000517:prevention & control*; D022121:Cholera Vaccines / Q000276:immunology*; D004751:Enteritis / Q000276:immunology / Q000382:microbiology; D006168:Guinea Pigs; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D019081:O Antigens / Q000276:immunology*; D011817:Rabbits; D014734:Vibrio cholerae / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure
D022121:Cholera Vaccines; D007106:Immune Sera; D019081:O Antigens