Inhibition by baclofen of NMDA-activated current in rat dorsal root ganglion neurons.
OBJECTIVE
To explore the modulatory effect of baclofen on NMDA-activated current in rat dorsal root ganglion (DRG) neurons.


METHODS
Whole-cell patch-clamp technique was used to record NMDA-activated current in isolated DRG neurons. Drugs were applied by rapid solution exchange.


RESULTS
Preapplication of baclofen 1-100 mumol.L-1 induced a concentration-dependent inhibition of the inward NMDA-activated current markedly. NMDA (100 mumol.L-1)-activated current was inhibited by 52% +/- 14% (n = 11, P < 0.01) by preapplication of baclofen 100 mumol.L-1. The inhibitory effect of baclofen was reversible, and was removed by saclofen 100 mumol.L-1, which was a selective antagonist of GABAB receptor.


CONCLUSIONS
Preapplication of baclofen exerts an inhibitory effect on NMDA-activated current in the primary sensory neurons.
Si
J Q
JQ
Research Center of Experimental Medicine, Tongji Medical University, Wuhan, China.
Li
Z W
ZW
D000818:Animals; D001418:Baclofen / Q000494:pharmacology*; D002469:Cell Separation; D004594:Electrophysiology; D005260:Female; D018755:GABA Agonists / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D008297:Male; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
D018755:GABA Agonists; D016202:N-Methylaspartate; D001418:Baclofen