title: Regioselective transformation of ciprofloxacin to N-acetylciprofloxacin by the fungus Mucor ramannianus.
abstract: A strain of the saprobic fungus Mucor ramannianus, isolated from a forest, was used to demonstrate the potential for ciprofloxacin biotransformation by zygomycetes in the environment. The fungus carried out the regioselective N-acetylation of ciprofloxacin to a single product, which was purified from culture extracts by high-performance liquid chromatography. The metabolite was identified by mass and nuclear magnetic resonance spectrometry as 1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(4-acetyl-1-piperazinyl)-3- quinolinecarboxylic acid.
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.
authors.forename: I A
authors.initials: IA
authors.lastname: Parshikov
authors.forename: J P
authors.initials: JP
authors.lastname: Freeman
authors.forename: J O
authors.initials: JO
authors.lastname: Lay
authors.forename: R D
authors.initials: RD
authors.lastname: Beger
authors.forename: A J
authors.initials: AJ
authors.lastname: Williams
authors.forename: J B
authors.initials: JB
authors.lastname: Sutherland
mesh_terms: D000107:Acetylation; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009089:Mucor / Q000378:metabolism*
chemical_list: D002939:Ciprofloxacin