Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050.
A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of approximately 80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.
Fraser
N J
NJ
Division of Biochemistry and Molecular Biology, IBLS, University of Glasgow, United Kingdom.
Dominy
P J
PJ
Ucker
B
B
Simonin
I
I
Scheer
H
H
Cogdell
R J
RJ
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D002021:Buffers; D002942:Circular Dichroism; D003902:Detergents; D004735:Energy Transfer; D005960:Glucosides; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D012241:Rhodopseudomonas / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D002021:Buffers; D003902:Detergents; D005960:Glucosides; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C023693:protein complex B800-850, Rhodobacter; C040358:dodecyl maltoside