An interaction between human colon carcinoma cells and hepatocytes activates transforming growth factor-beta1 in vitro.
We established in vitro heterotypic co-cultures of a human colon carcinoma cell line, HT-29, and a human hepatocyte line, tPH5CH, using transwells to investigate possible interactions between colon carcinoma cells and hepatocytes during the metastatic process. Co-culture, but not HT-29 conditioned medium, inhibited tPH5CH cell proliferation, and this inhibition was blocked by an anti-TGF-beta1 antibody. Significantly more activated TGF-beta1 was released by co-culture than by each cell line cultured alone, whereas there were no significant differences between the total TGF-beta1 released by the individual cultured cell lines and that released by co-culture. These data indicate that an interaction between human colon carcinoma cells and hepatocytes activates TGF-beta1 in vitro.
Nishibori
H
H
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Watanabe
M
M
Narai
S
S
Kubota
T
T
Matsubara
C
C
Teramoto
T
T
Kitajima
M
M
D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D016212:Transforming Growth Factor beta