Identification of the dimethylbenzyl mercapturic acid in urine of rats treated with 1,2,3-trimethylbenzene.
The structure was investigated of the mercapturic acid excreted in urine of rats after the i.p. administration of 1,2,3-trimethylbenzene. Of the two regioisomeric mercapturic acids, i.e. N-acetyl-S-(2,3-dimethylbenzyl)-L-cysteine and N-acetyl-S-(2,6-dimethyl-benzyl)-L-cysteine, only the former was isolated by preparative HPLC and identified, by comparison with an authentic specimen. The excretion rate of the mercapturate was estimated to be approximately 5% of dose, not a substantial metabolic route.
Tsujimoto
Y
Y
Osaka City Institute of Public Health and Environmental Sciences, Japan.
Noda
T
T
Shimizu
M
M
Moriwaki
H
H
Tanaka
M
M
D000111:Acetylcysteine / Q000737:chemistry / Q000652:urine*; D000818:Animals; D001555:Benzene Derivatives / Q000008:administration & dosage / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar
D001555:Benzene Derivatives; D004785:Environmental Pollutants; C010179:hemimellitene; D000111:Acetylcysteine