[Gastrointestinal stromal tumors: prognostic value of DNA analysis with flow cytometry and of classical clinicopathologic parameters].
BACKGROUND
Biological behavior of gastrointestinal stromal tumours (GIST) is still difficult to predict. The aim of this study was to analyze the prognostic valve of DNA study by flow cytometry in addition to the classical clinicopathologic parameters.


METHODS
82 GIST were investigated to assess by multivariable analysis the prognostic significance of classical morphologic and clinical features and DNA flow-cytometric analysis.


RESULTS
Features significantly correlated with bad prognosis were: pain, recurrences, metastasis, small intestine tumours, invasion of adjacent tissues, size > 6 cm, infiltrative pattern, high cellularity, high-grade of atypia, mitosis count > 2/50 high powered field, necrosis, DNA aneuploid-hyperploid or near-diploid, S and SG2M-phase of aneuploid/s population/s > 11% and average S-phase > 11%. Multivariable analysis showed that mitotic activity was the only independent variable indicating poor prognosis.


CONCLUSIONS
In this series of GIST mitosis count was the only independent prognostic factor, being more important than flow-cytometric variables like DNA ploidy and cellular cycle values.
Tarroch
X
X
Departamento de Anatomía Patológica, Hospitales Universitarios de la Vall d'Hebron, Barcelona. 24125xts@comb.es
Salas
A
A
Olona
M
M
Moragas
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm; D005260:Female; D005434:Flow Cytometry; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000401:mortality; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D015996:Survival Rate
D004273:DNA, Neoplasm
Tumores mesenquimales del tubo digestivo: valor pronóstico del análisis del ADN por citometría de flujo y de los parámetros clinicopatológicos clásicos.