title: Residual oil fly ash exposure enhances allergic sensitization to house dust mite.
abstract: Epidemiological studies have shown an association between elevated levels of particulate matter air pollution and increased morbidity and hospital visits in asthmatics. Residual oil fly ash (ROFA) is a primary combustion particle containing sulfate and metals such as vanadium, nickel, and iron. In this study the effect of ROFA on sensitization to house dust mite (HDM) was examined in a Brown Norway rat model of pulmonary allergy. Rats were instilled via the trachea with 200 or 1000 micrograms ROFA 3 days prior to local sensitization with 10 micrograms HDM and were challenged with 10 micrograms HDM 14 days later. Immunological endpoints were examined at 2, 7, and 14 days after sensitization and at 2 and 7 days after challenge (16 and 21 days post-sensitization, respectively). Antigen-specific immunoglobulin E and associated immediate bronchoconstriction responses to antigen challenge were increased in the ROFA-treated groups compared with the HDM control group. Lymphocyte proliferation to antigen was enhanced at Days 7 and 21 in the bronchial lymphocytes of ROFA-treated groups. Bronchoalveolar lavage fluid (BALF) eosinophil numbers and lactate dehydrogenase were significantly increased in the 1000 micrograms ROFA group at Days 2 and 16, BALF total proteins were elevated at Days 2 and 7 in both ROFA-treated groups, and BALF interleukin (IL)-10 was elevated in the 1000 micrograms ROFA group at Day 2. These results suggest that ROFA has an adjuvant effect on sensitization to HDM.
authors.affiliation: Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Lambert
authors.forename: W
authors.initials: W
authors.lastname: Dong
authors.forename: D W
authors.initials: DW
authors.lastname: Winsett
authors.forename: M K
authors.initials: MK
authors.lastname: Selgrade
authors.forename: M I
authors.initials: MI
authors.lastname: Gilmour
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D016084:Bronchoconstriction / Q000187:drug effects; D002244:Carbon / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D060729:Coal Ash; D004391:Dust / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008925:Mites / Q000276:immunology*; D052638:Particulate Matter; D014176:Protein Biosynthesis; D051381:Rats; D011914:Rats, Inbred BN
chemical_list: D000393:Air Pollutants; D060729:Coal Ash; D004391:Dust; D052638:Particulate Matter; D016753:Interleukin-10; D007073:Immunoglobulin E; D002244:Carbon; D007770:L-Lactate Dehydrogenase