Lipid monolayers: why use half a membrane to characterize protein-membrane interactions?
Variants of membrane-active proteins and peptides are increasingly available through synthesis and molecular engineering. When determining the effects of structural changes upon the interaction of these proteins with lipid membranes, monomolecular films of lipids at the air-water interface have significant advantages over bilayers and other lipid dispersions. In the past year, a variety of protein-lipid interactions has been characterized successfully using relatively simple surface measurements.
Brockman
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The Hormel Institute, University of Minnesota, 801 NE 16th Avenue, Austin, MN 55912, USA. hlbroc@maroon.tc.umn.edu
D000388:Air; D000818:Animals; D001671:Biochemistry / Q000379:methods; D002627:Chemistry, Physical / Q000379:methods; D003903:Deuterium; D005456:Fluorescent Dyes; D006801:Humans; D046911:Macromolecular Substances; D008563:Membrane Lipids / Q000378:metabolism*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism*; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D011312:Pressure; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D014867:Water
D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D008563:Membrane Lipids; D008565:Membrane Proteins; D008567:Membranes, Artificial; D014867:Water; D003903:Deuterium