Comparison of bilirubin conjugation in encapsulated hepatocytes, hepatocyte homogenate and intact hepatocytes.
Homogenized rat hepatocytes, whole hepatocytes and encapsulated hepatocytes were incubated with bilirubin and UDP-glucuronic acid to test their ability to form bilirubin conjugates. Bilirubin monoconjugated and diconjugated were detected in all the three preparations by HPLC analysis. The UDP-glucuronosyltransferase (UDPGT) activity of homogenized hepatocytes was 0.002 +/- 0.00006 mM/min per million cells; that of intact hepatocytes was 0.001 +/- 0.00006 mM/min per million cells and that of encapsulated hepatocytes was 0.0005 +/- 0.00002 mM/min per million cells.
Bruni
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Artificial Cells & Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Chang
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TM
D000464:Alginates; D000818:Animals; D001187:Artificial Organs; D001663:Bilirubin / Q000378:metabolism*; D002214:Capsules; D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008567:Membranes, Artificial; D011107:Polylysine; D051381:Rats; D017208:Rats, Wistar; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011107:Polylysine; D014535:Uridine Diphosphate Glucuronic Acid; D020723:Glucuronic Acid; D001663:Bilirubin