Adjuvant effect of anti-idiotypic antibodies to Yersinia pestis lipopolysaccharide.
Rabbit anti-idiotypic antibodies (anti-Id-ab) against Yersinia pestis lipopolysaccharide (LPS) were obtained with monoclonal immunoglobulins. Their complementary character to the original antigen was confirmed by immunohistochemical analysis and ELISA and gel precipitation tests. The anti-Id-ab were shown to possess all essential properties of Ab2beta subtype. Both in-vitro and in-vivo experiments demonstrated a pronounced adjuvant activity of anti-Id-ab without the toxic effect characteristic of Y. pestis LPS. Combined immunisation with anti-Id-ab plus the FI capsular antigen led to a significant increase in the protective immune response against experimental acute challenge with virulent Y. pestis.
Feodorova
V A
VA
Devdariani
Z L
ZL
Nazarova
L S
LS
D000276:Adjuvants, Immunologic; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010930:Plague / Q000517:prevention & control; D011817:Rabbits; D015010:Yersinia pestis / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000888:Antibodies, Anti-Idiotypic; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides