title: Chloramphenicol-sensitive mitochondrial translation in Trypanosoma brucei.
abstract: We developed an in organello system to label newly synthesized mitochondrially encoded proteins of Trypanosoma brucei. Highly purified mitochondria, prepared under isotonic conditions, were incubated with radioactive methionine and cysteine in a suitable translation buffer. Analysis of mitochondrial extracts on TRIS-Tricine gels revealed a subset of labeled, NP-40-insoluble proteins. The labeling of these proteins was resistant to the cytosol-specific translation inhibitor cycloheximide. The proteins, however, were not labeled in the presence of chloramphenicol or erythromycin, inhibitors of prokaryotic type translation, or puromycin, a general translation inhibitor. These results indicate that isotonically isolated mitochondria of T. brucei are capable of protein synthesis.
authors.affiliation: University of Fribourg, Institute of Zoology, Pérolles, Switzerland.
authors.forename: C E
authors.initials: CE
authors.lastname: Nabholz
authors.forename: D
authors.initials: D
authors.lastname: Speijer
authors.forename: A
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis; D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000096:biosynthesis*; D014346:Trypanosoma brucei brucei / Q000378:metabolism*
chemical_list: D011500:Protein Synthesis Inhibitors; D015800:Protozoan Proteins; D002701:Chloramphenicol; D000251:Adenosine Triphosphatases