Induction of antigen-specific immunosuppression by CD95L cDNA-transfected 'killer' dendritic cells.
Dendritic cells (DCs) are special subsets of antigen-presenting cells characterized by their highly potent capacity to activate immunologically naive T cells. Here we report that DCs that are transfected with CD95 ligand (CD95L) cDNA, called 'killer' DCs, deliver death signals, instead of activation signals, to T cells after antigen-specific interaction. Injection of antigen-pulsed killer DCs into mice before sensitization induced antigen-specific immunosuppression. When administered after sensitization, killer DCs suppressed immune responses almost completely after subsequent challenge. Thus, killer DCs represent an entirely new immunomodulatory protocol, which may become directly applicable in preventing and even treating T cell-mediated inflammatory diseases.
Matsue
H
H
Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235-9069, USA.
Matsue
K
K
Walters
M
M
Okumura
K
K
Yagita
H
H
Takashima
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D017690:Cell Transplantation; D002999:Clone Cells / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D017449:Dermatitis, Allergic Contact / Q000276:immunology; D004139:Dinitrofluorobenzene; D053222:Fas Ligand Protein; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance; D007111:Immunity, Cellular / Q000235:genetics; D008099:Liver / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D014162:Transfection; D019014:fas Receptor / Q000276:immunology
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D019014:fas Receptor; D010047:Ovalbumin; D004139:Dinitrofluorobenzene