title: Effect of NMDA receptor ligands on mast cell histamine release, a reappraisal.
abstract: Natural polyamines have been proposed to induce histamine release from mast cells through a direct interaction with G proteins. Alternatively, the polyamine binding site of ionotropic N-methyl-D-aspartate (NMDA) receptors has been suggested as a target for spermine on mast cells. We reexamined both hypotheses. Incubation of rat peritoneal mast cells with spermine resulted in a concentration-dependent histamine release (EC50 270 microM). Incubation with NMDA receptor agonists, glutamate or NMDA, associated to the co-agonist glycine, did not induce secretion. Western blot experiments did not reveal NMDA R1, R2a, R2b or R2c subunit expression in rat peritoneal mast cell membranes. The NMDA receptor antagonist at the glycine site, L-689,560, did not modify, at relevant concentrations, the spermine-induced secretion. The NMDA receptor antagonists, ifenprodil and LY 235959, and the NMDA channel blocker, MK801, slightly inhibited, at high concentrations, the secretory effect of spermine. The polyamine arcaine, an antagonist of the NMDA receptor polyamine binding site, induced histamine secretion (EC50 350 microM). Both spermine- and arcaine-induced effects were independent upon extracellular calcium and were largely inhibited by treatment of mast cells with pertussis toxin or benzalkonium chloride. The response to spermine and arcaine was prevented by the hydrolysis of sialic acid residues of the cell surface by neuraminidase, and was restored by permeabilization of the plasma membrane with streptolysine-O, indicating that polyamines act intracellularly. These results confirm the involvement of pertussis toxin-sensitive G proteins in the secretory effect of polyamines and demonstrate the absence of NMDA receptors on rat peritoneal mast cells. Nonselective effects of some NMDA receptor ligands on mast cells cannot be excluded.
authors.affiliation: Laboratoire de Neuroimmunopharmacologie, INSERM U425, Université Louis Pasteur Strasbourg I, Faculté de Pharmacie, Illkirch, France.
authors.forename: L
authors.initials: L
authors.lastname: Daeffler
authors.forename: K
authors.initials: K
authors.lastname: Nadra
authors.forename: V
authors.initials: V
authors.lastname: Eichwald
authors.forename: S
authors.initials: S
authors.lastname: Ohresser
authors.forename: Y
authors.initials: Y
authors.lastname: Landry
mesh_terms: D000818:Animals; D001645:Biguanides / Q000494:pharmacology; D015317:Biogenic Polyamines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008024:Ligands; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism / Q000494:pharmacology; D009439:Neuraminidase / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000187:drug effects*; D013096:Spermine / Q000494:pharmacology
chemical_list: D001645:Biguanides; D015317:Biogenic Polyamines; D008024:Ligands; D011956:Receptors, Cell Surface; D016194:Receptors, N-Methyl-D-Aspartate; D013096:Spermine; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C006624:arcaine; D009439:Neuraminidase; D019204:GTP-Binding Proteins; D019158:N-Acetylneuraminic Acid