A plasma cell leukemia patient showing bialleic 14q translocations: t(2;14) and t(11;14).
We report here an IgG/lambda-type plasma cell leukemia patient showing bialleic 14q32 translocations. All immunoglobulins were suppressed in this patient, but a small amount of monoclonal IgG was detected by immunoelectrophoresis. Two cells of six peripheral blood mononuclear cells showed 46,XY,t(2;14)(q11;q32), i(8)(q10), t(11;14)(q13;q32), del(12)(q13.1) by karyotypic analysis. We confirmed the juxtaposition of IgH and PRAD1/Cyclin D1 genes by fluorescent in situ hybridization and overexpression of the PRAD1/Cyclin D1 gene, but Southern analysis showed no bcl-1 rearrangement. We analyzed the t(2;14)(q11;q32) using DNA fragments derived from childhood B-chronic lymphocytic leukemia cases bearing t(2;14)(p13;q32). Southern and Northern analyses demonstrated no alteration of these genes, indicating that this t(2;14) was different from that of childhood B-chronic lymphocytic leukemia. At the IgH loci, Southern analysis showed two rearranged bands and one germ-line band of JH. Cmicro was deleted on one rearranged allele but remained on the other, suggesting that the chromosome translocation occurred after productive class switch recombination on the Cmicro deleted allele.
Sonoki
T
T
Second Department of Internal Medicine, Kumamoto University School of Medicine, Kumamoto, Japan.
Matsuzaki
H
H
Satterwhite
E
E
Nakazawa
N
N
Hata
H
H
Tucker
P W
PW
Taniwaki
M
M
Kuribayashi
N
N
Harada
N
N
Matsuno
F
F
Mitsuya
H
H
D000483:Alleles; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D005803:Genes, Immunoglobulin; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007146:Immunoglobulin lambda-Chains / Q000097:blood; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007952:Leukemia, Plasma Cell / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009710:Nucleotide Mapping; D014178:Translocation, Genetic / Q000235:genetics*
D007074:Immunoglobulin G; D007146:Immunoglobulin lambda-Chains