title: Genetic mechanisms of age regulation of human blood coagulation factor IX.
abstract: Blood coagulation capacity increases with age in healthy individuals. Through extensive longitudinal analyses of human factor IX gene expression in transgenic mice, two essential age-regulatory elements, AE5' and AE3', have been identified. These elements are required and together are sufficient for normal age regulation of factor IX expression. AE5', a PEA-3 related element present in the 5' upstream region of the gene encoding factor IX, is responsible for age-stable expression of the gene. AE3', in the middle of the 3' untranslated region, is responsible for age-associated elevation in messenger RNA levels. In a concerted manner, AE5' and AE3' recapitulate natural patterns of the advancing age-associated increase in factor IX gene expression.
authors.affiliation: Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-0618, USA. kkurachi@umich.edu
authors.forename: S
authors.initials: S
authors.lastname: Kurachi
authors.forename: Y
authors.initials: Y
authors.lastname: Deyashiki
authors.forename: J
authors.initials: J
authors.lastname: Takeshita
authors.forename: K
authors.initials: K
authors.lastname: Kurachi
mesh_terms: D020413:3' Untranslated Regions; D000375:Aging / Q000097:blood / Q000235:genetics*; D000818:Animals; D016384:Consensus Sequence; D018983:DNA Footprinting; D018900:Dinucleotide Repeats; D005164:Factor IX / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; D014157:Transcription Factors; C065788:transcription factor PEA3; D005164:Factor IX