Electrophoretic analysis of Bence Jones proteinuria.
The electrophoresis of Bence Jones proteinuria (BJP) by urinary protein electrophoresis (UPE), immunoelectrophoresis (IE), immunofixation electrophoresis (IFE), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF), two-dimensional electrophoresis (2-DE) and capillary electrophoresis (CE) is described. UPE, IE and IFE are briefly discussed as clinical laboratory methods for the detection and typing of free light chain (LC) whilst the high resolution electrophoretic methods (SDS-PAGE, IEF, 2-DE and CE) are considered in greater detail as research tools for molecular characterisation of free LC and its association with nephrotoxicity. Refinements of sample processing designed to improve the standardisation of analysis of BJP by high resolution electrophoretic methods are reported.
Marshall
T
T
Analytical Biochemistry Research Group, School of Health Sciences, The University of Sunderland, UK. tom.marshall@sunderland.ac.uk
Williams
K M
KM
D001536:Bence Jones Protein / Q000652:urine*; D004586:Electrophoresis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D011507:Proteinuria / Q000175:diagnosis*
D001536:Bence Jones Protein