The Kautsky curve is a built-in barcode.
We identify objects from their visually observable morphological features. Automatic methods for identifying living objects are often needed in new technology, and these methods try to utilize shapes. When it comes to identifying plant species automatically, machine vision is difficult to implement because the shapes of different plants overlap and vary greatly because of different viewing angles in field conditions. In the present study we show that chlorophyll a fluorescence, emitted by plant leaves, carries information that can be used for the identification of plant species. Transient changes in fluorescence intensity when a light is turned on were parameterized and then subjected to a variety of pattern recognition procedures. A Self-Organizing Map constructed from the fluorescence signals was found to group the signals according to the phylogenetic origins of the plants. We then used three different methods of pattern recognition, of which the Bayesian Minimum Distance classifier is a parametric technique, whereas the Multilayer Perceptron neural network and k-Nearest Neighbor techniques are nonparametric. Of these techniques, the neural network turned out to be the most powerful one for identifying individual species or groups of species from their fluorescence transients. The excellent recognition accuracy, generally over 95%, allows us to speculate that the method can be further developed into an application in precision agriculture as a means of automatically identifying plant species in the field.
Tyystjärvi
E
E
University of Turku, Department of Biology, Laboratory of Plant Physiology, BioCity A, Finland. esatyy@utu.fi
Koski
A
A
Keränen
M
M
Nevalainen
O
O
D055592:Biophysical Phenomena; D001703:Biophysics; D002734:Chlorophyll / Q000737:chemistry* / Q000528:radiation effects; D000077194:Chlorophyll A; D005453:Fluorescence; D008954:Models, Biological; D010363:Pattern Recognition, Automated; D010788:Photosynthesis; D010944:Plants / Q000737:chemistry* / Q000145:classification* / Q000528:radiation effects; D013045:Species Specificity
D002734:Chlorophyll; D000077194:Chlorophyll A