Toxicity of fatty acid 18:5n3 from Gymnodinium cf. mikimotoi: II. Intracellular pH and K+ uptake in isolated trout hepatocytes.
Effects of octadecapentaenoic acid 18:5n3 and other related polyunsaturated fatty acids present in gymnodinium cf. mikimotoi were tested in isolated trout hepatocytes. These exotoxins decreased intracellular pH followed by a slow recovery to initial value and alkalinization of acidic compartments, suggesting an inhibition of vacuolar H(+)-ATPases. Moreover, addition of 18:5n3 to the extracellular medium induced a decrease of K+ uptake into hepatocytes as a result of Na,K-ATPase inhibition. However, high concentrations (10(-5)-10(-3) M) are necessary to induce these effects.
Fossat
B
B
LPTE, Université de Nice-Sophia Antipolis, France. fossatb@unice.fr
Porthé-Nibelle
J
J
Sola
F
F
Masoni
A
A
Gentien
P
P
Bodennec
G
G
D000165:Acridine Orange / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004141:Dinoflagellida / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000633:toxicity*; D005456:Fluorescent Dyes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D000254:Sodium-Potassium-Exchanging ATPase; D000165:Acridine Orange; D011188:Potassium