title: Crystal structure of the copper chaperone for superoxide dismutase.
abstract: Cellular systems for handling transition metal ions have been identified, but little is known about the structure and function of the specific trafficking proteins. The 1.8 A resolution structure of the yeast copper chaperone for superoxide dismutase (yCCS) reveals a protein composed of two domains. The N-terminal domain is very similar to the metallochaperone protein Atx1 and is likely to play a role in copper delivery and/or uptake. The second domain resembles the physiological target of yCCS, superoxide dismutase I (SOD1), in overall fold, but lacks all of the structural elements involved in catalysis. In the crystal, two SOD1-like domains interact to form a dimer. The subunit interface is remarkably similar to that in SOD1, suggesting a structural basis for target recognition by this metallochaperone.
authors.affiliation: [1] Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois, 60208, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Lamb
authors.forename: A K
authors.initials: AK
authors.lastname: Wernimont
authors.forename: R A
authors.initials: RA
authors.lastname: Pufahl
authors.forename: V C
authors.initials: VC
authors.lastname: Culotta
authors.forename: T V
authors.initials: TV
authors.lastname: O'Halloran
authors.forename: A C
authors.initials: AC
authors.lastname: Rosenzweig
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: C093341:ATX1 protein, S cerevisiae; D002352:Carrier Proteins; D005656:Fungal Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D013482:Superoxide Dismutase