title: An in vitro study of the use of photodynamic therapy for the treatment of natural oral plaque biofilms formed in vivo.
abstract: Seven-day oral plaque biofilms have been formed on natural enamel surfaces in vivo using a previously reported in situ device. The devices are then incubated with a cationic Zn(II) phthalocyanine photosensitizer and irradiated with white light. Confocal scanning laser microscopy (CSLM) of the biofilms shows that the photosensitizer is taken up into the biomass of the biofilm and that significant cell death is caused by photodynamic therapy (PDT). In addition, the treated biofilms are much thinner than the control samples and demonstrate a different structure from the control samples, with little evidence of channels and a less dense biomass. Transmission electron microscopy (TEM) of the in vivo-formed plaque biofilms reveals considerable damage to bacteria in the biofilm, vacuolation of the cytoplasm and membrane damage being clearly visible after PDT. These results clearly demonstrate the potential value of PDT in the management of oral biofilms.
authors.affiliation: Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK. S.R.Wood@leeds.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Wood
authors.forename: B
authors.initials: B
authors.lastname: Nattress
authors.forename: J
authors.initials: J
authors.lastname: Kirkham
authors.forename: R
authors.initials: R
authors.lastname: Shore
authors.forename: S
authors.initials: S
authors.lastname: Brookes
authors.forename: J
authors.initials: J
authors.lastname: Griffiths
authors.forename: C
authors.initials: C
authors.lastname: Robinson
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003773:Dental Plaque / Q000188:drug therapy*; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000627:therapeutic use*; D054833:Isoindoles; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000627:therapeutic use*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry / Q000627:therapeutic use*; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine