title: Mouse dendritic cells express the P2X7 purinergic receptor: characterization and possible participation in antigen presentation.
abstract: Immune cells express P2 purinoceptors of the P2Y and P2X subtypes. In the present work, we show that three dendritic cell (DC) lines, D2SC/1, CB1, and FSDC, representative of immature DCs, express the P2X7 (formerly P2Z) receptor, as judged from RT-PCR amplification, reactivity to a specific antiserum, and pharmacological and functional evidence. Receptor expression is higher in FSDC cells, a cell line that is functionally more mature than D2SC/1 and CB1. From the wild-type DC population, we selected cell clones lacking the P2X7R (P2X7less). We also used a P2XR blocker, oxidized ATP, to irreversibly inhibit the P2X7R. Ability of P2X7less FSDCs or of oxidized ATP-inhibited FSDCs to stimulate Ag-specific TH lymphocytes was severely decreased although Ag endocytosis was minimally affected. During coculture with TH lymphocytes, wild-type FSDC secreted large amounts of IL-1beta. Release of this cytokine was reduced in P2X7less DCs. These data show that DCs express the P2X7 purinoceptor and suggest a correlation between P2X7R expression and Ag-presenting activity.
authors.affiliation: Section of General Pathology, Department of Experimental and Diagnostic Medicine, Biotechnology Center, University of Ferrara, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Mutini
authors.forename: S
authors.initials: S
authors.lastname: Falzoni
authors.forename: D
authors.initials: D
authors.lastname: Ferrari
authors.forename: P
authors.initials: P
authors.lastname: Chiozzi
authors.forename: A
authors.initials: A
authors.lastname: Morelli
authors.forename: O R
authors.initials: OR
authors.lastname: Baricordi
authors.forename: G
authors.initials: G
authors.lastname: Collo
authors.forename: P
authors.initials: P
authors.lastname: Ricciardi-Castagnoli
authors.forename: F
authors.initials: F
authors.lastname: Di Virgilio
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000276:immunology; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005333:Fetus; D007106:Immune Sera / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis* / Q000737:chemistry* / Q000276:immunology; D058486:Receptors, Purinergic P2X7; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology
chemical_list: D007106:Immune Sera; C546469:P2rx7 protein, mouse; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate; D002118:Calcium