title: [Heterologous expression of murine lymphotoxins in Escherichia coli and preparation of antibodies].
abstract: Genes encoding fragments of polypeptide chains of murine lymphotoxins (LT), namely, LT-alpha truncated from the N-terminus and the LT-beta extracellular domain, containing N-terminal hepta- and hexahistidine epitopes, respectively, were expressed in E. coli cells. The recombinant proteins purified by metallochelate chromatography were used to obtain polyclonal antibodies that specifically recognize murine LT.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Korobko
authors.forename: V E
authors.initials: VE
authors.lastname: Boĭchenko
authors.forename: D V
authors.initials: DV
authors.lastname: Kuprash
authors.forename: R L
authors.initials: RL
authors.lastname: Turetskaia
authors.forename: S A
authors.initials: SA
authors.lastname: Nedospasov
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006639:Histidine / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000276:immunology; D051379:Mice; D010957:Plasmids
chemical_list: D000906:Antibodies; D000939:Epitopes; D008233:Lymphotoxin-alpha; D006639:Histidine
vernacular_title: Geterologicheskaia ékspressiia limfotoksinov myshi v kletkakh Escherichia coli i poluchenie antitel k nim.