Local lymph node assay responses to paraphenylenediamine: intra- and inter-laboratory evaluations.
The murine local lymph node assay (LLNA) is a method for the prospective identification of skin sensitizing chemicals. Proliferative responses induced in lymph nodes draining the site of topical application of the test chemical are measured and those chemicals that induce a stimulation index of three or more compared with concurrent vehicle-treated controls are considered to have the potential to cause skin sensitization. Dose-response data from the LLNA may be used to derive an estimate of relative skin sensitizing potency, based upon derivation of the concentration of chemical required to cause a stimulation index of 3 (EC3 value) as calculated by linear interpolation. The purpose of the present investigations was to examine the stability of LLNA responses and the consistency of derived EC3 values induced by the contact allergen paraphenylenediamine (PPD). Analyses were conducted once a month over a 4-month period in each of two independent laboratories. In all assays, and in both laboratories, PPD elicited a positive response. Although some minor differences in responses between and within laboratories were observed, the derived EC3 values were generally very consistent. In Laboratory 1, EC3 values varied between 0.06 and 0.09% PPD, whereas in Laboratory 2 the range was 0.09-0.20%. These EC3 values are consistent with clinical experience of this material insofar as it is a common and relatively potent cause of allergic contact dermatitis in humans. Taken together, these data confirm the stability of LLNA responses both with time and between laboratories and provide additional support for the use of derived EC3 values in the assessment of relative skin sensitizing potency.
Warbrick
E V
EV
Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK. Vicky.Warbrick@CTL.ZENECA.com
Dearman
R J
RJ
Lea
L J
LJ
Basketter
D A
DA
Kimber
I
I
D000485:Allergens / Q000633:toxicity*; D000818:Animals; D002469:Cell Separation; D003877:Dermatitis, Contact / Q000209:etiology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D007753:Laboratories / Q000592:standards; D008198:Lymph Nodes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D010655:Phenylenediamines / Q000633:toxicity*; D011237:Predictive Value of Tests; D013997:Time Factors; D018675:Toxicity Tests
D000485:Allergens; D010655:Phenylenediamines; C029728:4-phenylenediamine