Establishment and growth regulation of a novel ovarian cancer cell line from a poorly-differentiated adenocarcinoma: proposal for a new treatment.
A continuously growing cultured cell line has been obtained in vitro, starting from a specimen of ascites fluid obtained from a patient with ovarian cancer, in whom a poorly-differentiated adenocarcinoma was diagnosed. This cell line, named OC-A1, is routinely grown in standard, serum-supplemented culture medium and has been fully stabilized to long-term growth and characterized for both cultural and genetic parameters. OC-A1 cells express a set of characteristics, as determined in vitro which, when compared with the in vivo primary tumor, confirm the high malignity of this cancer. In addition, karyotype analysis showed a translocation of chromosome 8 which is correlated with the amplification of c-myc oncogene. However, the expression of this oncogene was found to be significantly inhibited by a new regulatory activity, recently found to be present in a liposarcoma cell line. Conditioned medium from these cells was indeed able to inhibit the growth of OC-A1 cells, arresting their cell cycle in the G1 phase and inducing them to apoptosis. Finally, the cell programmed death appeared to be related to the expression of antioncogene p53.
Mancini
A
A
Experimental Oncology Dept. B, National Tumour Institute, Fondazione G. Pascale, Naples, Italy.
Borrelli
A
A
Formisano
S
S
Masucci
M T
MT
Maffeo
A
A
Perla
G
G
De Martino
L
L
Bevilacqua
N
N
Botti
G
G
Maggino
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D017209:Apoptosis; D002898:Chromosomes, Human, Pair 8; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D016259:Genes, myc; D006801:Humans; D008854:Microscopy, Electron; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure
D017077:Culture Media, Conditioned; D004273:DNA, Neoplasm