title: The sae locus of Staphylococcus aureus encodes a two-component regulatory system.
abstract: Sae is a regulatory locus that activates the production of several exoproteins in Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a probe adjacent to the transposon insertion of a sae::Tn551 mutant, was cloned into a bifunctional vector. This fragment was shown to carry the sae locus by restoration of exoprotein production in sae mutants. The sae locus was mapped to the SmaI-D fragment of the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the cloned fragment revealed the presence of two genes, designated saeR and saeS, encoding a response regulator and a histidine protein kinase, respectively, with high homology to other bacterial two-component regulatory systems.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico, Química y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
authors.forename: A T
authors.initials: AT
authors.lastname: Giraudo
authors.forename: A
authors.initials: A
authors.lastname: Calzolari
authors.forename: A A
authors.initials: AA
authors.lastname: Cataldi
authors.forename: C
authors.initials: C
authors.lastname: Bogni
authors.forename: R
authors.initials: R
authors.lastname: Nagel
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D015698:Genomic Library; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011494:Protein Kinases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011494:Protein Kinases; C527351:SaeS protein, Staphylococcus aureus