Protection against toxoplasmosis in mice immunized with different antigens of Toxoplasma gondii incorporated into liposomes.
Different toxoplasma antigens were entrapped within liposomes and evaluated, in this form, for their ability to protect Swiss mice against toxoplasma infection: soluble tachyzoite antigen (L/TAg), tissue cyst (L/CAg), tachyzoite plus tissue cyst (L/TCAg) or purified antigen of tachyzoite (L/pTAg). The protein used in L/pTAg was purified from tachyzoites using a stage-specific monoclonal antibody which reacted at a molecular weight of 32 kD in SDS PAGE and silver stain using reduced condition. To compare the immuno-adjuvant action of liposomes and of Freund's Complete Adjuvant (FCA), another group of mice was immunized with soluble tachyzoite antigen (STAg) emulsified in FCA (FCA/TAg). Control groups were inoculated with (STAg) alone, phosphate-buffered saline (PBS), FCA with PBS (FCA/PBS) and empty liposomes (L/PBS). Mice were inoculated subcutaneously with these antigens six, four and two weeks before a challenge with 80 tissue cysts of the P strain of Toxoplasma gondii orally. All mice immunized with or without adjuvant showed a humoral response, as measured by Elisa. However, no correlation was found between antibody titer and protection against the challenge. All mice immunized with L/pTAg or L/TCAg survived (100), whereas 80% and 90% of mice from groups which received respectively PBS or FCA/PBS and L/PBS died. All mice immunized with antigens entrapped within liposomes (L/TAg, L/CAg, L/TCAg and L/pTAg) showed low numbers of intracerebral cysts.
Elsaid
M M
MM
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brasil.
Vitor
R W
RW
Frézard
F J
FJ
Martins
M S
MS
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000302:isolation & purification; D004306:Dose-Response Relationship, Immunologic; D004337:Drug Carriers; D005260:Female; D008081:Liposomes; D051379:Mice; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000276:immunology / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D004337:Drug Carriers; D008081:Liposomes; D015800:Protozoan Proteins; D016052:Protozoan Vaccines