DNA analysis of malignant salivary gland carcinomas: comparison of different tissue preparations and measuring techniques.
This study correlates static and flow-cytometric analysis on paraffin-embedded and fresh material of salivary gland carcinomas. Nuclear suspensions for DNA analysis were prepared from paraffin-embedded and fresh material. Comparison of the results of static and flow cytometry on paraffin-embedded material revealed no significant correlation between DNA ploidy and S-phase value. The coefficients of variation were significantly lower for static than for flow cytometry (p < 0.05). Flow-cytometric analysis on fresh and paraffin-embedded material correlated well concerning DNA ploidy, but not for the S-phase. The coefficients of variation were significantly lower for the fresh than for the paraffin-embedded material (p < 0.001). Cytometric analysis on paraffin-embedded material of malignant salivary gland carcinomas should be critically evaluated. There is a need for flow-cytometric investigations on fresh material to clarify further the prognostic value of this method.
Nordgård
S
S
Department of Otolaryngology, University Hospital, University of Trondheim, Norway.
Franzén
G
G
Boysen
M
M
Tytor
M
M
D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D016612:Paraffin Embedding; D011003:Ploidies; D016196:S Phase; D012468:Salivary Gland Neoplasms / Q000235:genetics*; D012469:Salivary Glands / Q000737:chemistry
D004273:DNA, Neoplasm