title: Contribution of kasA analysis to detection of isoniazid-resistant Mycobacterium tuberculosis in Singapore.
abstract: Genotypic analysis of resistance to isoniazid (INH) in Mycobacterium tuberculosis is complex due to the various genes potentially involved. Mutations in ketoacyl acyl carrier protein synthase (encoded by kasA) were present in 16 of 160 (10%) INH-resistant isolates (R121K [n = 1], G269S [n = 3], G312S [n = 11], G387D [n = 1]). However, G312S was also present in 6 of 32 (19%) susceptible strains. kasA analysis contributed marginally to the performance of INH genotypic testing in Singapore. The significance of kasA polymorphisms in INH resistance should be carefully established.
authors.affiliation: Department of Clinical Research, Ministry of Health, Singapore General Hospital, Singapore 169608. gcrlsg@sgh.gov.sg
authors.forename: A S
authors.initials: AS
authors.lastname: Lee
authors.forename: I H
authors.initials: IH
authors.lastname: Lim
authors.forename: L L
authors.initials: LL
authors.lastname: Tang
authors.forename: A
authors.initials: A
authors.lastname: Telenti
authors.forename: S Y
authors.initials: SY
authors.lastname: Wong
mesh_terms: D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000235:genetics*; D000995:Antitubercular Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005838:Genotype; D007538:Isoniazid / Q000494:pharmacology*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012846:Singapore
chemical_list: D000995:Antitubercular Agents; C077402:3-ketoacyl-acyl carrier protein synthase III; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; D007538:Isoniazid