Differential changes in the properties of mitochondrial isoenzyme creatine kinase from heart of adult and old rats during stress.
We compared the effects of immobilization stress on the properties of mitochondrial creatine kinase (CK) isoenzyme from the myocardium of adult and old rats. Here we show that the activity of CK in the myocardium of old rats is lower in comparison to that in the myocardium of adult ones. During stress, CK activity in the myocardium of old rats increases, whereas in the myocardium of adults it does not. CK from the myocardium of adult rats is more sensitive to the modulating action of low pH, osmolarity, and Ca2+ concentrations than that of old rats.
Davydov
V V
VV
Department of Biochemistry, State Medical University, Zaporozhye, Ukraine. dav@reis.zp.ua
Shvets
V N
VN
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007103:Immobilization; D066298:In Vitro Techniques; D007527:Isoenzymes; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000201:enzymology
D007527:Isoenzymes; D000244:Adenosine Diphosphate; D003402:Creatine Kinase; D002118:Calcium