title: Quantification of lamivudine (3TC) by competitive immunoassay.
abstract: Lamivudine or 3TC, the (-) eniantiomer of 2'-deoxy-3'-thiacytidine, is a prototype of a novel class of levogyre dideoxynucleosides analogues used in treatment of HIV and HBV infection. We describe a method corresponding to the first enzyme immunoassay for quantifying this antiviral drug. This technique use an enzyme conjugate that not require the use of radioactive labelling. In this study, anti-3TC antibodies were raised in rabbits by immunising with 3TC-HS-kelhoyle limpet hemocyanin (KLH) conjugate.
authors.affiliation: Laboratoire de Chimie Bio-Organique, ESA CNRS 6001, Faculté des Sciences, Université de Nice-Sophia Antipolis, Nice, France.
authors.forename: F
authors.initials: F
authors.lastname: Akeb
authors.forename: C
authors.initials: C
authors.lastname: Creminon
authors.forename: M C
authors.initials: MC
authors.lastname: Nevers
authors.forename: J
authors.initials: J
authors.lastname: Grassi
authors.forename: D
authors.initials: D
authors.lastname: Duval
authors.forename: R
authors.initials: R
authors.lastname: Guedj
mesh_terms: D019380:Anti-HIV Agents / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D007118:Immunoassay / Q000379:methods*; D019259:Lamivudine / Q000032:analysis* / Q000276:immunology; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis* / Q000276:immunology
chemical_list: D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D019259:Lamivudine