On the mechanism of background silver staining during sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox initiator system using ammonium persulphate (APS) and an amine such as N,N,N',N'-tetramethylethylenediamine (TEMED).
Patras
G
G
Department of Chemical Engineering, The University of Melbourne, Parkville, Australia.
Qiao
G G
GG
Solomon
D H
DH
D000645:Ammonium Sulfate; D004591:Electrophoresis, Polyacrylamide Gel; D005029:Ethylenediamines; D010084:Oxidation-Reduction; D016622:Silver Staining / Q000379:methods*
D005029:Ethylenediamines; C005798:N,N,N',N'-tetramethylethylenediamine; C031276:ammonium peroxydisulfate; D000645:Ammonium Sulfate