Three new types of Apaf-1 in mammalian cells.
Apaf-1, the human C. elegans Ced-4 homologue, plays a crucial role in mitochondrial mediated apoptosis. To determine whether a mutant Apaf-1 is involved in the pathogenesis of malignant lymphomas, we performed sequence analysis of its transcriptional gene product. Therefore, cDNAs coding for Apaf-1 were examined in six lymphoma derived cell lines, in three non-lymphoid tumor cell lines, as well as in peripheral blood lymphocytes and in tissue from heart, kidney, and liver by RT-PCR. These studies did not disclose the expected tumor related alterations but led instead to the identification of three novel forms of Apaf-1. These forms vary in length, but contain all functional domains formerly characterized in the Apaf-1 protein. The results indicate that the previously published sequence is not the Apaf-1 form most widely expressed.
Hahn
C
C
Institut für Pathologie, UK Benjanin Franklin, Freie Universität Berlin, Hindenburgdamm 30, Berlin, 12200, Germany.
Hirsch
B
B
Jahnke
D
D
Dürkop
H
H
Stein
H
H
D000595:Amino Acid Sequence; D017209:Apoptosis; D053477:Apoptotic Protease-Activating Factor 1; D006689:Hodgkin Disease / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008223:Lymphoma / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured
C506592:APAF1 protein, human; D053477:Apoptotic Protease-Activating Factor 1; D011506:Proteins