Origin, kinetics, and function of chimeric B lymphocytes in liver allografts.
BACKGROUND
Despite the well-recognized concordance of chimerism with spontaneous acceptance of rat liver allografts, the active role and the identity of chimeric cells mediating liver allograft tolerance are unknown. Because resting B cells are endowed with a tolerogenic antigen-presenting capacity, we assessed whether donor B cells propagated from the grafted liver may be responsible for liver allograft tolerance.


METHODS
Dark Agouti or Lewis rats were grafted with Lewis or Dark Agouti livers as a tolerogenic or a rejection combination, respectively. We followed the kinetics of donor B cells in recipients by flow cytometry, and we examined the fate of liver allografts depleted of passenger B cells in either B cell-sufficient or -deficient recipients. B-cell depletion was achieved by treatment of animals with polyclonal goat anti-rat IgM antibody from birth.


RESULTS
During the first 3 days after liver allografting, donor B cells rapidly migrated from graft-infiltrating cells and appeared in systemic circulation in both the tolerogenic and rejection combinations. However, systemic chimerism was detectable in the tolerogenic combination by day 14, whereas it was undetectable in the rejection combination by day 7. In graft-infiltrating cells, a significant expansion of chimeric IgM+ (newly formed) B cells was observed on day 5 in the tolerogenic, but not in the rejection, combination. However, depletion of B cells from liver grafts and the absence of antibodies failed to alter the outcome of liver allograft survival in the tolerogenic or immunogenic combination.


CONCLUSIONS
Although intragraft chimeric B cells proliferated in tolerogenic liver allografts, their clonal expansion does not seem to be essential for the promotion of liver allograft tolerance.
Yokoi
Y
Y
Department of Surgery, University of Pennsylvania Medical Center, Philadelphia 19104, USA.
Noorchashm
H
H
Rostami
S Y
SY
Barker
C F
CF
Naji
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D006085:Graft Survival / Q000502:physiology; D007108:Immune Tolerance; D007075:Immunoglobulin M / Q000032:analysis; D000922:Immunotoxins / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D000922:Immunotoxins