title: Comparison of the ability of dopamine receptor agonists to inhibit forskolin-stimulated adenosine 3'5'-cyclic monophosphate (cAMP) accumulation via D2L (long isoform) and D3 receptors expressed in Chinese hamster ovary (CHO) cells.
abstract: The pharmacological properties of the human D2L (long isoform) and rat D3 dopamine receptors in functional assays were examined. A range of dopamine agonists were assessed for their ability to inhibit adenosine 3'5'-cyclic monophosphate (cAMP) accumulation via the two receptors expressed stably in Chinese hamster ovary cells. Dopamine caused a significantly greater maximal inhibition (P < 0.05) of cAMP accumulation via the D2L receptor (approximately 70%) as compared to the D3 receptor (approximately 50%). The pattern of agonist effects was different at the two receptors. The absolute and relative potencies for inhibition of cAMP accumulation were different for a range of agonists acting at the two receptors. Similarly, the maximal inhibitions achieved by a range of agonists were different for the two receptors.
authors.affiliation: Research School of Biosciences, University of Kent, Canterbury, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Hall
authors.forename: P G
authors.initials: PG
authors.lastname: Strange
mesh_terms: D000818:Animals; D016466:CHO Cells; D005576:Colforsin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology*; D006801:Humans; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D050637:Receptors, Dopamine D3; D014162:Transfection
chemical_list: C493593:DRD3 protein, human; D018491:Dopamine Agonists; C493595:Drd3 protein, rat; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D005576:Colforsin; D000242:Cyclic AMP; D004298:Dopamine