title: Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication.
abstract: Chromosomes are replicated in characteristic, temporal patterns during S phase. We have compared the timing of association of replication proteins at early- and late-replicating origins of replication. Minichromosome maintenance proteins assemble simultaneously at early- and late-replicating origins. In contrast, Cdc45p association with late origins is delayed relative to early origins. DNA polymerase alpha association is similarly delayed at late origins and requires Cdc45p function. Activation of the S phase checkpoint inhibits association of Cdc45p with late-firing origins. These studies suggest that Cdc45p is poised to serve as a key regulatory target for both the temporal and checkpoint-mediated regulation of replication origins.
authors.affiliation: Massachusetts Institute of Technology, Department of Biology, Room 68-622, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: O M
authors.initials: OM
authors.lastname: Aparicio
authors.forename: A M
authors.initials: AM
authors.lastname: Stout
authors.forename: S P
authors.initials: SP
authors.lastname: Bell
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics*; D004256:DNA Polymerase I / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D016193:G1 Phase; D006238:Haploidy; D007700:Kinetics; D008938:Mitosis; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C102209:CDC45 protein, S cerevisiae; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D004256:DNA Polymerase I