Two-step synergism between the progesterone receptor and the DNA-binding domain of nuclear factor 1 on MMTV minichromosomes.
In contrast to its behavior as naked DNA, the MMTV promoter assembled in minichromosomes can be activated synergistically by the progesterone receptor and NF1 in a process involving ATP-dependent chromatin remodeling. The DNA-binding domain of NF1 is required and sufficient for stable occupancy of all receptor-binding sites and for functional synergism. Activation of purified minichromosomes is observed in the absence of SWI/SNF and can be enhanced by recombinant ISWI. Receptor binding to minichromosomes recruits ISWI and NURF38, but not brahma. We propose a two-step synergism in which the receptor triggers a chromatin remodeling event that facilitates access of NF1, which in turn stabilizes an open nucleosomal conformation required for efficient binding of further receptor molecules and full transactivation.
Di Croce
L
L
IMT, Institut für Molekularbiologie und Tumorforschung Philipps-Universität Marburg, Germany.
Koop
R
R
Venditti
P
P
Westphal
H M
HM
Nightingale
K P
KP
Corona
D F
DF
Becker
P B
PB
Beato
M
M
D000251:Adenosine Triphosphatases / Q000235:genetics; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000235:genetics*; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000196:embryology; D029721:Drosophila Proteins; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000235:genetics; D011401:Promoter Regions, Genetic; D011755:Pyrophosphatases; D011980:Receptors, Progesterone / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C086468:ISWI protein; D019476:Insect Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009707:Nucleosomes; D011980:Receptors, Progesterone; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C086469:brm protein, Drosophila; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; C115568:Nurf-38 protein, Drosophila