title: Affinity capillary electrophoresis coupled with on-line microdialysis by attachable electrode.
abstract: This paper developed a new affinity capillary electrophoresis method coupled with on-line microdialysis using attachable electrodes in order to study the interaction of biomacromolecules with their small molecule ligands in the desired physiological conditions. The sampling mechanism was discussed and the experimental conditions were optimized. The affinity constant of sulfamethoxazole (SMZ) with bovine serum albumin (BSA) was determined with homemade equipment. The method is simple and practical.
authors.affiliation: Laboratory of Analytical Chemistry in Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing.
authors.forename: M
authors.initials: M
authors.lastname: Fang
authors.forename: L
authors.initials: L
authors.lastname: Sheng
authors.forename: H
authors.initials: H
authors.lastname: Han
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.forename: B
authors.initials: B
authors.lastname: Zang
authors.forename: G
authors.initials: G
authors.lastname: Liu
mesh_terms: D002138:Calibration; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D017551:Microdialysis / Q000379:methods*; D012710:Serum Albumin, Bovine; D013420:Sulfamethoxazole
chemical_list: D012710:Serum Albumin, Bovine; D013420:Sulfamethoxazole