title: Epitope-dependent synergism and antagonism between CD40 antibodies and soluble CD40 ligand for the regulation of CD23 expression and IgE synthesis in human B cells.
abstract: BACKGROUND
  The induction of IgE synthesis in naive B cells requires two T-cell-derived signals: one delivered through CD40 and the other via interleukin-4 (IL-4). The natural counterstructure to CD40 is the CD40 ligand (CD40L). We have asked about the interplay between CD40L and CD40 mAb that recognize distinct epitopes in delivering signals for regulating IL-4-dependent IgE synthesis and the expression of CD23, the low-affinity IgE receptor, in resting B cells.
  
  
  METHODS
  After culture of purified human tonsillar B cells with CD40 agonists and IL-4, surface CD23 was determined by flow cytometric analysis. CD23 levels in cell lysates and supernatants were quantified by ELISA, as were those of secreted IgE.
  
  
  RESULTS
  With regard to both induction of CD23 and IgE production, soluble CD40L trimer (sCD40LT) showed synergistic interaction with two mAb to CD40 which bind to epitopes located outside the ligand binding site (EA5 and 5C3), but not with a mAb (G28-5) which effectively competes for CD40L binding to CD40. Each of the two noncompeting mAb to CD40 was able to cooperate strongly with sCD40LT in promoting high-level induction of CD23 even in the absence of IL-4, an effect mirrored in the promotion of strong homotypic clustering and high-rate DNA synthesis. G28-5, uniquely, induced a down-regulation in IL-4-induced CD23 expression with time, a change that was accompanied by an increase in the amount of soluble CD23 detected. While the two noncompeting mAb consistently synergized with sCD40LT for the promotion of IL-4-dependent IgE synthesis, sCD40LT and G28-5 (which, by itself, was the most potent of the CD40 mAb at inducing IL-4-dependent IgE production) exhibited mutual antagonism in this regard, the level of which could be quite profound.
  
  
  CONCLUSIONS
  This study demonstrates that appropriate targeting of CD40 can modulate IgE synthesis either positively or negatively.
authors.affiliation: MRC Centre for Immune Regulation, University of Birmingham, Edgbaston, UK.
authors.forename: A
authors.initials: A
authors.lastname: Challa
authors.forename: J D
authors.initials: JD
authors.lastname: Pound
authors.forename: R J
authors.initials: RJ
authors.lastname: Armitage
authors.forename: J
authors.initials: J
authors.lastname: Gordon
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology*; D023201:CD40 Ligand; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000276:immunology*; D017455:Receptors, IgE / Q000096:biosynthesis*
chemical_list: D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D018985:Epitopes, B-Lymphocyte; D008024:Ligands; D008562:Membrane Glycoproteins; D017455:Receptors, IgE; D023201:CD40 Ligand; D007073:Immunoglobulin E