title: [Cross neutralization of bothrops jararacussu venom by heterologous antivenoms].
abstract: We have studied the immunochemical cross-reactivity and cross-neutralization of the lethal potency, hemorrhagic, necrotizing, procoagulant and (indirect) hemolytic activities of Bothrops jararacussu venom by the standard antivenoms produced in Argentina. These antivenoms are horse immunoglobulin F (ab')2 fragments from animals immunized with 1) Crotalus durissus terrificus venom (Monovalent Anticrotalic antivenom); 2) Bothrops alternatus and B. neuwiedii venoms (Bivalent Botropic antivenom); 3) B. alternatus, B. neuwiedii, B. jararaca and B. jararacussu venoms (Tetravalent Bothropic, or "Misiones" antivenom) and 4) B. alternatus, B. neuwiedii and C. d. terrificus venoms (Trivalent Botropic-Crotalic antivenom). In preincubation experiments, all the heterologous antivenoms neutralized the toxic and biological activities of B. jararacussu venom with a potency at least as high as the Tetravalent Botropic (i.e. the only homologous) antivenom, in which B. jararacussu venom was included as immunogen. These results suggest the possibility of using heterologous antibothropic antivenoms for the treatment of snake bites by B. jararacussu.
authors.affiliation: Instituto Nacional de Producción de Biológicos ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud, Buenos Aires, Argentina. aderoodt@ba.net
authors.forename: A R
authors.initials: AR
authors.lastname: de Roodt
authors.forename: J C
authors.initials: JC
authors.lastname: Vidal
authors.forename: S
authors.initials: S
authors.lastname: Litwin
authors.forename: J C
authors.initials: JC
authors.lastname: Dokmetjian
authors.forename: J A
authors.initials: JA
authors.lastname: Dolab
authors.forename: S E
authors.initials: SE
authors.lastname: Hajos
authors.forename: L
authors.initials: L
authors.lastname: Segré
mesh_terms: D000818:Animals; D000997:Antivenins / Q000627:therapeutic use*; D017837:Bothrops; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000008:administration & dosage / Q000276:immunology*; D009500:Neutralization Tests; D051381:Rats; D012909:Snake Bites / Q000628:therapy*
chemical_list: D000997:Antivenins; D003435:Crotalid Venoms
vernacular_title: Neutralización cruzada de veneno de Bothrops jararacussu por sueros antiofidicos heterologos.