title: Polyamine oxidase and tissue transglutaminase activation in rat small intestine by polyamines.
abstract: Polyamine degradation was studied in the small intestine from rats fed on a polyamine-supplemented diet. Lactalbumin diet was given to Hooded-Lister rats, with or without 5 mg rat(-1) day(-1) of putrescine or spermidine for 5 days. Polyamine oxidase activity increased with putrescine and spermidine in the diet, whereas spermidine/spermine N(1)-acetyltransferase and diamine oxidase activities were unchanged. We also studied the calcium-dependent and -independent tissue transglutaminase activities, since they can modulate intestinal polyamine levels. Both types of enzymes increased in the cytosolic fraction after putrescine (about 65%) or spermidine (80-100%). Our results indicate that exogenous polyamines stimulate intestinal polyamine oxidase and tissue transglutaminase activities, probably to prevent polyamine accumulation, when other pathways of polyamine catabolism (acetylation and terminal catabolism) are not activated.
authors.affiliation: Dipartimento di Scienze Precliniche L.I.T.A. di Vialba, Università degli Studi di Milano, Via G.B. Grassi 74, 20157, Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Tunici
authors.forename: A
authors.initials: A
authors.lastname: Sessa
authors.forename: E
authors.initials: E
authors.lastname: Rabellotti
authors.forename: G
authors.initials: G
authors.lastname: Grant
authors.forename: S
authors.initials: S
authors.lastname: Bardocz
authors.forename: A
authors.initials: A
authors.lastname: Perin
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000284:Administration, Oral; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004032:Diet; D004789:Enzyme Activation / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D007768:Lactalbumin / Q000008:administration & dosage; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D011073:Polyamines / Q000032:analysis / Q000494:pharmacology*; D011700:Putrescine / Q000008:administration & dosage; D051381:Rats; D013095:Spermidine / Q000008:administration & dosage; D011503:Transglutaminases / Q000378:metabolism*; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D006631:Amine Oxidase (Copper-Containing); D007768:Lactalbumin; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D011700:Putrescine; D013095:Spermidine; D011503:Transglutaminases; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase