title: Induction of hepatocyte proliferation and prevention of hepatocyte apoptosis by phenobarbital related to local humoral factor in mouse liver.
abstract: OBJECTIVE
  To study the association of phenobarbital (Phe) inducing hepatocyte proliferation and blocking hepatocyte apoptosis with local humoral factor in liver.
  
  
  METHODS
  The ratio of liver/body weight, DNA content, regressive rate of hyperplastic liver, and DNA fragmentation were used to investigate whether the Phe-treated mouse liver extract (PMLE) and PMLE-95 (PMLE heated at 95 degrees C for 30 min) possessed Phe-like effects on mouse liver. Meantime, the effects of pretreatment with trypsin, RNAase, and DNAase on the activity of PMLE-95 were observed, and the differences of components between PMLE-95 and NMLE-95 (normal mouse liver extract, NMLE heated at 95 degrees C for 30 min) were analyzed with HPLC.
  
  
  RESULTS
  PMLE-95 stimulated hepatocyte proliferation and prevented hepatocyte apoptosis caused by withdrawing Phe in mice, and the activity of PMLE-95 was eliminated after the pretreatment with trypsin. On the chromatograms PMLE-95 had 5 main peaks, while NMLE-95 had only 4 peaks.
  
  
  CONCLUSIONS
  The effects of Phe on the liver were mediated by an intrinsic protein or peptide substance produced in response for the stimulation of Phe in mouse liver.
authors.affiliation: Eastern Institute of Hepatobiliary Surgery, Second Military Medical University, Shanghai, China. ixweik@online.sh.cn
authors.forename: P
authors.initials: P
authors.lastname: He
authors.forename: F
authors.initials: F
authors.lastname: Shen
authors.forename: M C
authors.initials: MC
authors.lastname: Wu
authors.forename: L F
authors.initials: LF
authors.lastname: Li
authors.forename: Y J
authors.initials: YJ
authors.lastname: Guo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D008099:Liver / Q000166:cytology*; D008110:Liver Extracts / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010634:Phenobarbital / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D008110:Liver Extracts; D010634:Phenobarbital