Assessing chromosome 22 microdeletions in meningioma with new polymorphic DNA markers.
In an approach to identify new candidates for tumor related genes, a set of established and new polymorphic DNA markers (simple sequence repeats) was used in menigioma samples to test for deletions within human chromosome 22. For this purpose, cytogenetically well characterized tumors were applied and those with monosomy #22 were omitted; only disomic material was analyzed. When comparing DNA from peripheral blood and tumor tissues using markers spaced throughout the long arm (22q) no allelic loss was observed indicating that, in disomic samples, deletions are present as microdeletions undetectable by the marker spacing chosen in this study, or that they do not occur frequently.
Subke
F
F
Institut fur Humangenetik, Universitat des Saarlandes, D-66421 Homburg/Saar, Germany.
Pusch
C
C
Schroder
K
K
Meese
E
E
Blin
N
N
D014408:Biomarkers, Tumor; D002872:Chromosome Deletion; D002892:Chromosomes, Human, Pair 22; D004273:DNA, Neoplasm / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers