title: Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis transmission.
abstract: Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochís vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.
authors.affiliation: Núcleo de Medicina Tropical, Universidade de Brasília, Brasília, DF, 70919-970, Brasil. gromero@unb.br
authors.forename: G A
authors.initials: GA
authors.lastname: Romero
authors.forename: R N
authors.initials: RN
authors.lastname: Sampaio
authors.forename: V de O
authors.initials: Vde O
authors.lastname: Macedo
authors.forename: P D
authors.initials: PD
authors.lastname: Marsden
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle; D001938:Brazil; D003470:Culture Media; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D007892:Leishmania braziliensis / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D008297:Male; D012189:Retrospective Studies; D012424:Rural Population; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media