Endothelin and mechanical properties of the carotid artery in Wistar-Kyoto and spontaneously hypertensive rats.
The aim of this study is to evaluate the role of endothelin in the control of the static mechanical properties of in vitro carotid arteries from 14-week-old Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). An in vitro preparation in which the artery was allowed to longitudinally elongate similarly to the in situ carotid artery was employed. The diameter of in vitro carotid arteries subjected to static pressures (from 25 to 200 mmHg in 25 mmHg steps) was determined by videomicroscopy and computer-assisted image analysis, the cross-sectional compliance- and distensibility-pressure curves being then derived. The role of endothelin was assessed by incubating carotid arteries with the selective ETA and ETB endothelin receptor antagonists BQ123 and BQ788, respectively. These effects were compared with those observed under control conditions, as well as with those following complete abolition of vascular smooth muscle tone by potassium cyanide (KCN). Carotid diameter was significantly larger, and compliance and distensibility significantly smaller, in SHR compared to WKY rats. Local incubation with BQ123 was associated with significant dilations as well as significant increases in cross-sectional compliance and distensibility in both strains. This was even more pronounced with KCN, while BQ788 had no effect. The results of the present study suggest that: (i) endothelin exerts a tonic stiffening effect on the in vitro common carotid artery; (ii) this effect is mediated via the ETA endothelin receptor, and (iii) the stiffening effect of endothelin is exerted to a similar extent in the carotid arteries of normotensive WKY and SHR rats.
Marano
G
G
Laboratorio di Farmacologia, Istituto Superiore di Sanitá, Rome, Italy. gmarano@net.iss.it
Grigioni
M
M
Palazzesi
S
S
Ferrari
A U
AU
D000818:Animals; D001696:Biomechanical Phenomena; D017536:Carotid Artery, Common / Q000187:drug effects* / Q000502:physiology; D065128:Endothelin Receptor Antagonists; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D009842:Oligopeptides / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014741:Video Recording
D065128:Endothelin Receptor Antagonists; D009842:Oligopeptides; D010456:Peptides, Cyclic; D010880:Piperidines; C086539:BQ 788; C072247:cyclo(Trp-Asp-Pro-Val-Leu)