title: Pyrimidine nucleotidases/phosphotransferases from human erythrocyte.
abstract: Two cytoplasmic pyrimidine 5'-nucleotidase have been purified from human erythrocytes to homogeneity and partially characterized. The two enzymes, indicated as PN-I and PN-II, preferentially hydrolyse pyrimidine 5'-monophosphates and 3'-monophosphates, respectively. The kinetic analysis demonstrate that pyrimidine 5'-nucleotidases, in the presence of suitable nucleoside substrates, can operate as phosphotransferases by transferring phosphate to various nucleoside acceptors, including nucleoside analogues known as important drugs widely used in chemotherapy.
authors.affiliation: Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Amici
authors.forename: M
authors.initials: M
authors.lastname: Emanuelli
authors.forename: N
authors.initials: N
authors.lastname: Raffaelli
authors.forename: S
authors.initials: S
authors.lastname: Ruggieri
authors.forename: G
authors.initials: G
authors.lastname: Magni
mesh_terms: D015720:5'-Nucleotidase / Q000097:blood*; D002384:Catalysis; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D010766:Phosphorylation; D010770:Phosphotransferases / Q000097:blood*
chemical_list: D010770:Phosphotransferases; D015720:5'-Nucleotidase