[Measurements of serum and subretinal fluid before and after surgery of retinal detachment].
OBJECTIVE
To investigate the relationships between proliferative vitreoretinopathy (PVR) formation and the levels of human epidermal growth factor (h-EGF) and tumor necrosis factor (TNF) in serum and subretinal fluid (SRF) before and after surgery of retinal detachment with PVR.


METHODS
Serum and SRF samples of 51 patients with retinal detachment and PVR were collected, and their h-EGF and TNF levels were measured by radioimmunoassays.


RESULTS
In comparison with the control group, the levels of h-EGF and TNF in serum and SRF in retinal detachment with PVR group were higher but not statistically significant (P > 0.05). Their levels in the serum were not related to the severity of the disease, while their levels in SRF increased significantly with the increase of the severity of the disease, and these two factors were positively correlated (P < 0.05). 5 days after the surgery of retinal detachment, the h-EGF and TNF levels in serum increased significantly (P < 0.01) and the levels decreased within 30 days but not to normal.


CONCLUSIONS
The h-EGF and TNF in SRF might have a synergic action and affect the formation and development of PVR. The h-EGF and TNF in serum take part in the repair of the operative trauma and inflammatory reaction. However, their high levels could interfere with the effect of operation.
Jin
S
S
Air Force Wulumuqi Hospital.
Wang
Y
Y
Jiang
G
G
D000328:Adult; D004815:Epidermal Growth Factor / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005122:Exudates and Transudates / Q000378:metabolism; D005260:Female; D006801:Humans; D007432:Intraoperative Period; D008297:Male; D008875:Middle Aged; D012163:Retinal Detachment / Q000378:metabolism* / Q000601:surgery; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor