title: Cellular calcium handling in brain slices from calbindin D28k-deficient mice.
abstract: Cellular calcium handling was examined in brain slices from transgenic antisense mice with a regional deficiency in the neuronal calcium binding protein calbindin D28k and from their non transgenic wild type litter mate controls. Depolarization of brain slices with NMDA or potassium produced a prolonged elevation of neuronal calcium signal in neurons in brain slices from calbindin D28k-deficient transgenic mice. This effect was selective and was seen only in brain areas where the antisense construct produced a significant depletion of calbindin D28k protein. In other regions where calbindin D28k protein was not modified by the construct and in all glial cells whether from wild type or transgenic mice, cellular calcium handling was normal.
authors.affiliation: Department of Experimental Biomedical Sciences, University of Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Pasti
authors.forename: G
authors.initials: G
authors.lastname: Carmignoto
authors.forename: T
authors.initials: T
authors.lastname: Pozzan
authors.forename: R
authors.initials: R
authors.lastname: Battini
authors.forename: S
authors.initials: S
authors.lastname: Ferrari
authors.forename: G
authors.initials: G
authors.lastname: Lally
authors.forename: P C
authors.initials: PC
authors.lastname: Emson
mesh_terms: D000818:Animals; D016375:Antisense Elements (Genetics) / Q000235:genetics; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D012016:Reference Values; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism*
chemical_list: D016375:Antisense Elements (Genetics); C576499:Calb1 protein, mouse; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D002118:Calcium