Expression of bovine interleukin-2 in insect cells by recombinant baculovirus.
Bovine interleukin-2 (bIL-2) was expressed in insect cells infected with recombinant baculovirus. The cDNA for bIL-2 was amplified by reverse transcriptional polymerase chain reaction and recombinant baculovirus was constructed by homologous recombination. The recombinant baculovirus was plaque-purified, amplified, and then infected to Sf9 cells for expression of recombinant bIL-2 (rbIL-2). In result, the protein band corresponding to rbIL-2 could be detected on SDS-PAGE with coomassie brilliant blue staining and on immunoblot analysis reacted with mouse antiserum against bIL-2. In addition, cell proliferation assay for bIL-2 activity demonstrated that the culture supernatant of Sf9 cells infected with recombinant baculovirus enhanced the proliferation of bovine peripheral blood mononuclear cells in a dose-dependent manner.
Kashima
T
T
Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.
Iwata
H
H
Inoue
T
T
D000818:Animals; D016367:Baculoviridae; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D007274:Injections, Intraperitoneal; D007313:Insecta / Q000166:cytology; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D007376:Interleukin-2