Analysis by enzyme-linked immunosorbent assay and PCR of human liver abscess aspirates from patients in China for Entamoeba histolytica.
Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) revealed that abscesses were caused by various strains.
Zengzhu
G
G
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Bracha
R
R
Nuchamowitz
Y
Y
Cheng-I
W
W
Mirelman
D
D
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008100:Liver Abscess / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D011817:Rabbits
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan