Responses of microglia and neural progenitors to mechanical brain injury.
Neural cells have distinct responses to CNS injury; however, neural progenitor response to CNS injury is not yet documented. Stab injury combined with injection of bromodeoxyuridine (BrdU), a thymidine analog, into adult rat cortex above the lateral ventricle for 10 min resulted in activated microglia/macrophage infiltration along with nuclear factor kappa B (NF kappa B)/p65 activation at the lesion site. Most NF kappa B/p65+ cells displayed a phagocytic morphology. Under these conditions, profound cell apoptosis took place in the injured corpus callosum, but not in the subventricular/ventricular zone (SVZ/VZ). The SVZ/VZ-derived neural progenitors in both injected and non-injected contralateral hemispheres showed strong BrdU immunostaining, indicating that SVZ/VZ-derived neural progenitors of both hemispheres may undergo DNA synthesis in response to unilateral brain injury.
Tzeng
S F
SF
Department of Research and Education, Taichung Veterans General Hospital, Taiwan, R.O.C.
Wu
J P
JP
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D002135:Calcium-Binding Proteins; D002552:Cerebral Ventricles / Q000378:metabolism / Q000473:pathology; D004247:DNA / Q000096:biosynthesis; D008264:Macrophages / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology; D017628:Microglia / Q000473:pathology / Q000502:physiology*; D016328:NF-kappa B / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000502:physiology; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000502:physiology*; D050863:Synaptotagmin I; D050857:Synaptotagmins; D013997:Time Factors
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D050863:Synaptotagmin I; D050857:Synaptotagmins; D004247:DNA