title: Reasons and limits of substrate activity of modified L-dNTP in DNA biosynthesis.
abstract: Theoretical and experimental analysis of interaction of modified D- and L- dNTP as substrates for template-dependent and template-independent DNA polymerases was performed. It is shown that if the modified nucleoside 5'-triphosphates do not contain a substituent in position 3' DNA chains can be extended by both strereoisomeric series with the same kinetic parameters. But the presence of even a 3'-hydroxy group in L-dNTP prevents their incorporation into the DNA chain.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Krayevsky
authors.forename: N B
authors.initials: NB
authors.lastname: Dyatkina
authors.forename: D G
authors.initials: DG
authors.lastname: Semizarov
authors.forename: L S
authors.initials: LS
authors.lastname: Victorova
authors.forename: E A
authors.initials: EA
authors.lastname: Shirokova
authors.forename: F
authors.initials: F
authors.lastname: Theil
authors.forename: M J
authors.initials: MJ
authors.lastname: Von Janta Lipinski
authors.forename: G
authors.initials: G
authors.lastname: Gosselin
authors.forename: J L
authors.initials: JL
authors.lastname: Imbach
mesh_terms: D004261:DNA Replication; D009711:Nucleotides / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009711:Nucleotides