title: Characterization of muscle sarcoplasmic and myofibrillar protein hydrolysis caused by Lactobacillus plantarum.
abstract: Strains of Lactobacillus plantarum originally isolated from sausages were screened for proteinase and aminopeptidase activities toward synthetic substrates; on the basis of that screening, L. plantarum CRL 681 was selected for further assays on muscle proteins. The activities of whole cells, cell extracts (CE), and a combination of both on sarcoplasmic and myofibrillar protein extracts were determined by protein, peptide, and free-amino-acid analyses. Proteinase from whole cells initiated the hydrolysis of sarcoplasmic proteins. The addition of CE intensified the proteolysis. Whole cells generated hydrophilic peptides from both sarcoplasmic and myofibrillar proteins. Other peptides of a hydrophobic nature resulted from the combination of whole cells and CE. The action of both enzymatic sources on myofibrillar proteins caused maximal increases in lysine, arginine, and leucine, while the action of those on sarcoplasmic proteins mainly released alanine. In general, pronounced hydrolysis of muscle proteins required enzyme activities from whole cells in addition to those supplied by CE.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46100 Burjassot, Valencia, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Fadda
authors.forename: Y
authors.initials: Y
authors.lastname: Sanz
authors.forename: G
authors.initials: G
authors.lastname: Vignolo
authors.forename: M
authors.initials: M
authors.lastname: Aristoy
authors.forename: G
authors.initials: G
authors.lastname: Oliver
authors.forename: F
authors.initials: F
authors.lastname: Toldrá
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000626:Aminopeptidases / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007778:Lactobacillus / Q000201:enzymology* / Q000302:isolation & purification; D008297:Male; D008461:Meat Products / Q000382:microbiology; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine
chemical_list: D000596:Amino Acids; D009124:Muscle Proteins; D010450:Endopeptidases; D000626:Aminopeptidases