title: Postoperative exposure to glove powders modulates production of peritoneal eicosanoids during peritoneal wound healing.
abstract: OBJECTIVE
  To assess the effect of postsurgical exposure of peritoneal cavity to glove powders, Hydrocote, latex proteins, and lipopolysaccharide (LPS) on eicosanoid production in peritoneal fluid and cellular distribution of eicosanoid enzymes in peritoneal wound during healing.
  
  
  METHODS
  Randomised experimental study.
  
  
  METHODS
  Institute for Wound Research, USA.
  
  
  METHODS
  360 mice randomised into six groups of 60 each.
  
  
  METHODS
  Abrasion of peritoneal cavity followed by instillation of 500 microl of sterile phosphate buffered saline (PBS) alone (Control) or containing 100 microg/ml of Biosorb, Keoflo, Hydrocote, 1 mg/ml of latex proteins, or 12.5 microg/ml of LPS. Mice were killed at 1, 2, 4, 7, 14 and 28 days, and the peritoneal washing obtained from each animal and concentration of eicosanoids measured. Tissue were immunostained for cyclooxygenases and 5-lipoxygenase and thromboxane A2 (TXA2) synthetase.
  
  
  RESULTS
  Peritoneal fluid from uninjured controls contained 3.9 (0.8), 5.2 (0.3) and 0.2 (0.02) ng/ml of thromboxane B2 (TXB2), prostaglandin E2 (PGE2) and leukotriene B4 (LTB4), respectively. These increased significantly during the first week to 6.3 (0.3), 11.7 (0.8) and 2.6 (0.1) ng/ml, p<0.05, before returning to baseline by day 14. In all the treated groups the values were significantly higher than in controls (p<0.05). Immunoreactive cyclo-oxygenases, 5-lipoxygenase and TXA2 synthetase proteins were present in various cell types in uninjured skin and peritoneum, incisional and peritoneal wounds and adhesion tissues. Staining was more intense at the site of wounds and paralleled eicosanoid concentrations during healing. There was no difference between exposed and unexposed groups.
  
  
  CONCLUSIONS
  The presence of glove powders, latex proteins and LPS in peritoneal cavity cause increased eicosanoid production and aggravate the normal inflammatory reaction to tissue injury. This may contribute to the inflammatory or immune reactions and development of adhesions caused by glove powders.
authors.affiliation: Department of Obstetrics and Gynecology, Institute for Wound Research, University of Florida, Gainesville 32610, USA.
authors.forename: N
authors.initials: N
authors.lastname: Chegini
authors.forename: H
authors.initials: H
authors.lastname: Rong
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry; D015777:Eicosanoids / Q000032:analysis / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005932:Gloves, Surgical; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D010532:Peritoneal Diseases / Q000201:enzymology; D010537:Peritoneum / Q000187:drug effects* / Q000201:enzymology* / Q000293:injuries; D011184:Postoperative Period; D011208:Powders / Q000494:pharmacology*; D011897:Random Allocation; D013997:Time Factors; D000267:Tissue Adhesions / Q000201:enzymology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D015777:Eicosanoids; D011208:Powders