In vitro survival and germination of Candida albicans in the presence of nitrogen compounds.
The in vitro effect of nitric oxide (NO) and nitrite on blastoconidia and hyphae of Candida albicans was studied. Sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) were used as NO donors. Both minimal and complex media at two pH values, 7.0 and 4.5, were used for the assays. Blastoconidia were more susceptible than hyphae to NO. The NO effect on blastoconidia was greater at acidic pH. Nitrite affected the viability of blastoconidia in complex medium. The percentage germination and the relative rate of elongation of hyphae were both enhanced when NO was present in acidic conditions.
Abaitua
Fernando
F
Rementería
Aitor
A
Millan
Rosario San
RS
Eguzkiza
Ainhoa
A
Rodriguez
Jose Antonio
JA
Pontón
José
J
Sevilla
María Jesús
MJ
D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D009599:Nitroprusside / Q000378:metabolism; D010396:Penicillamine / Q000031:analogs & derivatives / Q000378:metabolism; D012977:Sodium Nitrite / Q000378:metabolism / Q000494:pharmacology*; D013172:Spores, Fungal / Q000502:physiology
D003470:Culture Media; C110881:S-nitro-N-acetylpenicillamine; D009599:Nitroprusside; D009569:Nitric Oxide; D010396:Penicillamine; D012977:Sodium Nitrite