title: Labeled carcinoembryonic antigen antibodies excitable by infrared rays: a novel diagnostic method for micro cancers in the digestive tract.
abstract: OBJECTIVE
  An indocyanine green derivative (ICG-sulfo-OSu) was used as the labeling substance for monoclonal antibody, and a fluorescence imaging system appropriate for ICG-sulfo-OSu excitable by infrared rays (IR) was developed. The goal of this study was to demonstrate antibody labeling at the tissue level using this new imaging system.
  
  
  METHODS
  ICG-sulfo-OSu labeled mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody, a newly developed imaging system, and an infrared ray microscope were employed in this experiment. Paraffin sections of human colon cancer previously proven to have cross-reactivity to anti-CEA antibody were examined.
  
  
  RESULTS
  Positive staining was seen as a brownish discoloration of oxidized 3,3'-diaminobenzidine tetrahydrochloride (DAB) in sections that reacted with ICG-sulfo-OSu-labeled anti-CEA antibody, and the fluorescence was well-matched with the oxidized DAB-positive sites.
  
  
  CONCLUSIONS
  Specific antibodies labeled with ICG-sulfo-OSu have significant affinity to cancer cells and seem to reflect sufficient amounts of fluorescence by IR to be useful in a system for the endoscopic detection of micro cancers using the immunohistochemical staining method.
authors.affiliation: Second Department of Internal Medicine, The University of Tokushima.
authors.forename: N
authors.initials: N
authors.lastname: Muguruma
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Bando
authors.forename: S
authors.initials: S
authors.lastname: Taoka
authors.forename: Y
authors.initials: Y
authors.lastname: Kusaka
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: S
authors.initials: S
authors.lastname: Ichikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Matsunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Tada
authors.forename: S
authors.initials: S
authors.lastname: Okamura
authors.forename: K
authors.initials: K
authors.lastname: Ii
authors.forename: K
authors.initials: K
authors.lastname: Imaizumi
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Takesako
authors.forename: S
authors.initials: S
authors.lastname: Shibamura
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000276:immunology; D003429:Cross Reactions; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D007208:Indocyanine Green / Q000031:analogs & derivatives; D007259:Infrared Rays; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D016612:Paraffin Embedding
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes; C113792:indocyanine green-sulfo-OSu; D007208:Indocyanine Green