title: Uniform band intensities in fluorescent dye terminator sequencing.
abstract: The use of Cyanine dye (Cy5 and Cy5.5) labeled dideoxy terminators with Thermo Sequenase DNA polymerase in DNA sequencing provides uniform band intensity, improved sequence read-length, and accuracy. It also greatly improves the ability to detect single base heterozygotes with dye-terminator sequencing method.
authors.affiliation: Amersham Pharmacia Biotech, Cleveland, OH 44128, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: C W
authors.initials: CW
authors.lastname: Fuller
authors.forename: S
authors.initials: S
authors.lastname: Nampalli
authors.forename: M
authors.initials: M
authors.lastname: Khot
authors.forename: I
authors.initials: I
authors.lastname: Livshin
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Hamilton
authors.forename: S B
authors.initials: SB
authors.lastname: Samols
authors.forename: J A
authors.initials: JA
authors.lastname: Mamone
authors.forename: K M
authors.initials: KM
authors.lastname: Hujer
authors.forename: B F
authors.initials: BF
authors.lastname: McArdle
authors.forename: J R
authors.initials: JR
authors.lastname: Nelson
authors.forename: S
authors.initials: S
authors.lastname: Duthie
mesh_terms: D001483:Base Sequence; D004247:DNA; D005456:Fluorescent Dyes / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D004247:DNA; D019914:Taq Polymerase