[The inhibitory effect in vitro of salvia miltiorrhiza and tetramethyl pyrazine on the growth of fibroblasts].
In order to investigate the inhibitory effect of salvia miltiorrhiza (SM) and tetramethyl pyrazine (TP) on scartricial fibroblast, the hypertrophic scar tissue of chest was chosen for culture of fibroblasts, and the influence of SM and TP on fibroblasts was observed, The effect of the drugs on the growth of fibroblasts, on DNA synthesis of fibroblasts and on mitosis index of fibroblasts were all determined quantitatively. The results showed: 1. SM and TP could inhibit significantly the growth of the fibroblasts, the inhibitory effect was irreversible when the concentration of the drugs reached 5 mg/ml and 500 micrograms/ml respectively; 2. SM and TP could inhibit the absorption of 3H-TdR and this effect was correlated positively to the dosage of the drugs and; 3. SM and TP could reduce the mitosis index of fibroblasts. It was concluded that SM and TP had definite depressive effect on growth of fibroblasts which was correlated positively with the concentration of drugs and duration of application. The inhibitory effect of the drugs on fibroblasts was mainly through inhibition of synthesis of DNA.
Shang
Q
Q
Department of Plastic Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, P.R. China.
Zhang
D
D
Guan
W
W
D002056:Burns / Q000150:complications*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000209:etiology / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D008940:Mitotic Index / Q000187:drug effects; D010936:Plant Extracts; D011719:Pyrazines / Q000494:pharmacology*; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011719:Pyrazines; D004247:DNA; C017953:tetramethylpyrazine