Identification of a gene encoding a human oxysterol-binding protein-homologue: a potential general molecular marker for blood dissemination of solid tumors.
This study describes a new potential role in human cancer for a gene, HLM, isolated by differential display, that bears homology to an oxysterol-binding protein. A significant association between increased expression of HLM with metastatic disease was found. HLM mRNA levels were increased in circulating tumor cells in patients' peripheral blood and in primary human epithelial cells expressing the human papillomavirus16 E6 and E7 proteins. HLM mRNA was not detected in most normal human tissues, including peripheral blood and lymph node. These findings indicate that HLM may function as a potential marker for tumor dissemination.
Fournier
M V
MV
Cell Growth and Regulation Division, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA. fournier@mbcrr.harvard.edu
Guimarães da Costa
F
F
Paschoal
M E
ME
Ronco
L V
LV
Carvalho
M G
MG
Pardee
A B
AB
Giumaraes
F C
FC
D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D001940:Breast / Q000166:cytology / Q000378:metabolism; D001943:Breast Neoplasms / Q000737:chemistry; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D004847:Epithelial Cells / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating; D009856:Oncogene Proteins, Viral / Q000502:physiology; D027383:Papillomaviridae / Q000235:genetics; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D011987:Receptors, Steroid / Q000097:blood / Q000235:genetics*; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000098825:Oxysterol Binding Proteins
D014408:Biomarkers, Tumor; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011987:Receptors, Steroid; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D000098825:Oxysterol Binding Proteins