Influence of growth conditions on the production of xylanolytic enzymes by Aspergillus flavus.
Investigations were carried out to optimize the culture conditions for the production of xylanase and beta-xylosidase by Aspergillus flavus, a filamentous fungus isolated from soil. The production of enzymes was tolerant to a wide range of initial culture pH values. Maximum xylanase (190 U/ml) and beta-xylosidase (35 U/ml) production was obtained when the strain was grown on mineral medium supplemented with 3% (w/v) corn cob powder as the carbon source. The enzymes had optimal activities at pH values between 5.5 and 6.0 and exhibited high activity and stability under alkaline conditions.
de Souza
C G
CG
Departamento de Bioquímica, Universidade Estadual de Maringá, PR, Brazil.
Girardo
N S
NS
Costa
M A
MA
Peralta
R M
RM
D001231:Aspergillus flavus / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000378:metabolism*
D003470:Culture Media; D005656:Fungal Proteins; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C026579:exo-1,4-beta-D-xylosidase