title: Molecular cloning and expression of Arabidopsis fatty acid hydroperoxide lyase.
abstract: Fatty acid hydroperoxide lyase (HPOL), an enzyme of the octadecanoid pathway that forms carbon-6 aldehydes such as n-hexanal or (Z)-3-hexenal, was cloned from Arabidopsis thaliana as a full-length cDNA. The HPOL activity obtained by expressing the cDNA in Escherichia coli formed n-hexanal from linoleic acid 13-hydroperoxide, whereas linoleic acid 9-hydroperoxide was not a substrate for the enzyme. The HPOL mRNA is expressed at low level in leaves; however, its accumulation can be found in the inflorescence. Wounding or methyl jasmonate treatments increase the mRNA level in leaves. These results indicate that the HPOL gene is up-regulated in leaves in response to wounding and that the enzyme may be an active component of the octadecanoid defense response.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan. matsui@agr.yamaguchi-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Matsui
authors.forename: J
authors.initials: J
authors.lastname: Wilkinson
authors.forename: B
authors.initials: B
authors.lastname: Hiatt
authors.forename: V
authors.initials: V
authors.lastname: Knauf
authors.forename: T
authors.initials: T
authors.lastname: Kajiwara
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D003577:Cytochrome P-450 Enzyme System; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase