Enantiometric separation of amino acids by copper (II)-L-arginine ligand exchange capillary zone electrophoresis.
A novel method for the separation of amino acid enantiomers by ligand exchange capillary zone electrophoresis (CZE) using a chiral Cu(II)-L-arginine ligand was developed in this paper. The effects of the buffer concentration, pH, the molar ratio of L-arginine to Cu(II), and the total concentrations of Cu(II) and L-arginine on the migration times and resolutions of chiral amino acids were investigated. The optimum experimental conditions were 40 mmol/L of NH4OAc, pH 7.0, the 2:1 concentration ratio of L-arginine to Cu(II), 1 mmol/L CuSO4 x 5H2O and 2 mmol/L L-arginine. Using the proposed method, six different dansyl-amino acids were separated successfully with the resolution ranging from 1.1 to 2.3 in less than 15 min.
Yuan
Z
Z
Department of Chemistry, Graduate School, USTC, Chinese Academy of Sciences, Beijing. yangll@es1.gsbustc.ac.cn
Yang
L
L
Zhang
S
S
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D001120:Arginine; D002021:Buffers; D003300:Copper; D003619:Dansyl Compounds; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008024:Ligands
D000596:Amino Acids; D002021:Buffers; D003619:Dansyl Compounds; D008024:Ligands; D003300:Copper; D001120:Arginine