Discrimination of multidrug-resistant Mycobacterium tuberculosis IS6110 fingerprint subclusters by rpoB gene mutation analysis.
The rpoB gene mutations in a 69-bp region of the gene, resulting in resistance to rifampin, were used to discriminate between Mycobacterium tuberculosis IS6110 fingerprint subclusters. These subclusters exhibited identical IS6110 fragments or had one or two additional fragments. In the two major subclusters all the analyzed strains have the same variant rpoB allele but are different from each other, suggesting the occurrence of independent outbreaks.
Portugal
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I
Unidade dos Retrovírus e Infecções Associadas and Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, 1649-019 Lisbon, Portugal. isabel.portugal@ff.ul.pt
Maia
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S
Moniz-Pereira
J
J
D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases; D018432:Drug Resistance, Multiple; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology
D004251:DNA Transposable Elements; D010940:Plant Proteins; C101305:RpoB protein, Oryza sativa; D012321:DNA-Directed RNA Polymerases; D007538:Isoniazid; D012293:Rifampin; D013307:Streptomycin