Characteristics of recombinant human butyrylcholinesterase.
OBJECTIVE
To study the biochemical-pharmacological properties of the recombinant human butyrylcholinesterase (rhBChE) and thereby to size up the potential possibility of using it as a detoxifying agent in succinylcholine intoxication.


METHODS
CHO-dhfr cells were transfected with plasmids by electroporation. BChE activity was determined colorimetrically by 5, 5'-dithiobis-(2-nitrobenzoic acid) (DTNB) method. Antigenicity was estimated by enzyme-linked immunosorbent assay and Western blot.


RESULTS
The maximal expression amounted to 25.83 ng.h-1/10(6) cells. The rhBChE was highly similar to the native human BChE (nhBChE) in terms of its catalytic property, substrate affinity, inhibitor sensitivity, reactivation, stability, and immunoreactivity with anti-nhBChE antibodies. Mice challenged with 1.5 lethal dose of succinylcholine preincubated with rhBChE survived without any symptoms of intoxication.


CONCLUSIONS
The rhBChE and nhBChE exhibit similar biochemical-pharmacological features. It is of potential value in practical use.
Yuan
H J
HJ
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
Yu
W Y
WY
Shi
C H
CH
Sun
M J
MJ
D000818:Animals; D002091:Butyrylcholinesterase / Q000235:genetics* / Q000378:metabolism* / Q000627:therapeutic use; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D018274:Electroporation; D051379:Mice; D010957:Plasmids; D011041:Poisoning / Q000517:prevention & control; D011994:Recombinant Proteins / Q000378:metabolism / Q000627:therapeutic use; D013390:Succinylcholine / Q000506:poisoning; D014162:Transfection
D011994:Recombinant Proteins; D002091:Butyrylcholinesterase; D013390:Succinylcholine