title: Hydrolysis of some mRNA 5'-cap analogs catalyzed by the human Fhit protein--and lupin ApppA hydrolases.
abstract: Hydrolysis of the following four cap analogs: m7G(5')ppp(5')A, m7G(5')ppp(5')m6A, m7G(5')ppp(5')m2'OG and m7G(5')ppp(5')2'dG catalyzed by homogeneous human Fhit protein and yellow lupin Ap3A hydrolase has been investigated. The hydrolysis products were identified by HPLC analysis and the K(m) and Vmax values calculated based on the data obtained by the fluorimetric method.
authors.affiliation: Department of Biophysics, University of Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Bojarska
authors.forename: R
authors.initials: R
authors.lastname: Kraciuk
authors.forename: J
authors.initials: J
authors.lastname: Wierzchowski
authors.forename: Z
authors.initials: Z
authors.lastname: Wieczorek
authors.forename: J
authors.initials: J
authors.lastname: Stepiński
authors.forename: M
authors.initials: M
authors.lastname: Jankowska
authors.forename: E
authors.initials: E
authors.lastname: Starzyńska
authors.forename: A
authors.initials: A
authors.lastname: Guranowski
authors.forename: E
authors.initials: E
authors.lastname: Darzynkiewicz
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009363:Neoplasm Proteins; D010944:Plants / Q000201:enzymology; D011506:Proteins / Q000378:metabolism*; D012315:RNA Caps; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D011506:Proteins; D012315:RNA Caps; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000099170:Bis(5'-adenosyl)triphosphatase