Effect of pH on Ki-67 antigen retrieval in renal cell carcinoma.
The efficacy of Ki-67 antigen retrieval (AR) was assessed in 20 formalin fixed-paraffin embedded specimens of renal cell carcinoma using three solutions with different pH values. The AR was performed by means of microwave oven heating up to 97 degrees C for 15 minutes. Better results were obtained using solutions with pH values 8.0 and 9.9 compared with AR in a solution of pH 6.0, which is routinely used in daily pathological practice. Individual testing of various AR conditions is recommended to obtain the best immunohistochemical results.
Masera
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A
Institute of Pathology, Medical Faculty, University of Ljubljana, Slovenia.
Stanisa
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O
Ovcak
Z
Z
Cör
A
A
D002292:Carcinoma, Renal Cell / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000276:immunology*
D019394:Ki-67 Antigen