The effect of methylprednisolone on intracellular calcium of normal and dystrophic human skeletal muscle cells.
Clinical trials have shown that a glucocorticoid, the methyiprednisolone (PDN), has a beneficial effect on muscle strength and function in Duchenne muscular dystrophy (DMD) patients. The aim of this study was to test if the effect of PDN could be mediated via a possible action on intracellular calcium. The intracellular calcium activity, at rest and during calcium mobilizing drug superfusion protocols was recorded in normal and dystrophic human cocultured muscle cells. PDN (10 microM) pretreatment induced an elevation of the resting calcium concentration of 51, 34 and 38% in proliferating normal myoblasts, DMD myoblasts and DMD myotubes, respectively, while normal myotubes resting [Ca2+]i was not altered.
Vandebrouck
C
C
Laboratoire de Biomembranes et Signalisation Cellulaire, CNRS UMR 6558, Université de Poitiers, Poitiers, France. phygen@campus.univ-poitiers.fr
Imbert
N
N
Duport
G
G
Cognard
C
C
Raymond
G
G
D000293:Adolescent; D000328:Adult; D002118:Calcium / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005938:Glucocorticoids / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D008775:Methylprednisolone / Q000494:pharmacology / Q000627:therapeutic use*; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000378:metabolism*
D005938:Glucocorticoids; D002118:Calcium; D008775:Methylprednisolone