Distinct inhibitory effects of 2-chloro-2'-deoxyadenosine and 9-beta-D-arabinosyl-2-fluoroadenine on DNA methyltransferase in human T-lymphocytes.
The effect of 2-chloro-2'-deoxyadenosine and 9-beta-D-arabinosyl-2-fluoroadenine on DNA methyltransferase activity in stimulated human T-lymphocytes was estimated. In comparative studies 5-aza-deoxycytidine and deoxyadenosine plus deoxycoformycin were used. These antileukemic compounds demonstrated different effects; both 2CdA and dAdo plus dCF, like 5-aza-dCyt, inhibited the enzyme activity by 85-90% after 72 hours activation of lymphocytes, while the effect of F-ara-A, under the same conditions, was insignificant.
Wyczechowska
D
D
Department of General Chemistry, Medical University of Lodz, Poland.
Ruckemann
K
K
Duley
J A
JA
Simmonds
A H
AH
Fabianowska-Majewska
K
K
D017338:Cladribine / Q000494:pharmacology*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D013601:T-Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D014740:Vidarabine / Q000031:analogs & derivatives* / Q000494:pharmacology
D004791:Enzyme Inhibitors; D017338:Cladribine; D004248:DNA (Cytosine-5-)-Methyltransferases; D014740:Vidarabine; C024352:fludarabine