Comparison and recovery of Escherichia coli and thermotolerant coliforms in water with a chromogenic medium incubated at 41 and 44.5 degrees C.
This study compared the performance of a commercial chromogenic medium, CHROMagarECC (CECC), and CECC supplemented with sodium pyruvate (CECCP) with the membrane filtration lauryl sulfate-based medium (mLSA) for enumeration of Escherichia coli and non-E. coli thermotolerant coliforms (KEC). To establish that we could recover the maximum KEC and E. coli population, we compared two incubation temperature regimens, 41 and 44.5 degrees C. Statistical analysis by the Fisher test of data did not demonstrate any statistically significant differences (P = 0.05) in the enumeration of E. coli for the different media (CECC and CECCP) and incubation temperatures. Variance analysis of data performed on KEC counts showed significant differences (P = 0.01) between KEC counts at 41 and 44.5 degrees C on both CECC and CECCP. Analysis of variance demonstrated statistically significant differences (P = 0.05) in the enumeration of total thermotolerant coliforms (TTCs) on CECC and CECCP compared with mLSA. Target colonies were confirmed to be E. coli at a rate of 91.5% and KEC of likely fecal origin at a rate of 77.4% when using CECCP incubated at 41 degrees C. The results of this study showed that CECCP agar incubated at 41 degrees C is efficient for the simultaneous enumeration of E. coli and KEC from river and marine waters.
Alonso
J L
JL
Instituto de Hidrologia y Medio Natural, Universidad Politecnica, 46022 Valencia, Spain. jalonso@ihdr.upv.es
Soriano
A
A
Carbajo
O
O
Amoros
I
I
Garelick
H
H
D001431:Bacteriological Techniques; D002863:Chromogenic Compounds; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D004926:Escherichia coli / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D005618:Fresh Water / Q000382:microbiology; D006801:Humans; D012623:Seawater / Q000382:microbiology; D013696:Temperature; D014871:Water Microbiology
D002863:Chromogenic Compounds; D003470:Culture Media