Allelic variation in the intron 2 and 3 of the MICA gene.
We have determined by sequencing the allelic variation in intron 2 and 3 of hte MICA gene for a total of 22 different alleles. Sequencing of introns was performed in two directions, using DNA from homozygous cell lines from families and from unrelated individuals. Intron 2 is 273 bp long and did in the alleles investigated not reveal any length polymorphism. We found a total of eight polymorphic positions which exhibit a strict biallelism, as it is also found in the polymorphisms for exon 2, 3 and 4 of MICA. Intron 3 is 586 bp long an required an additional set of primers placed near the middle of this intron in order to allow a complete bidirectional sequence. In intron 3, a total of 10 polymorphic positions were identified. Interestingly, we found two variants of the allele MICA*002 which are distinguished only by one basepair difference in intron 3. The variant MICA*002A is associated with HLA-B35 and B58, while the allele MICA*002B is associated with B38 and B39.
Yao
Z
Z
Kinderpoliklinik, LMU München, Germany.
Volgger
A
A
Keller
E
E
Chandanayingyong
D
D
Albert
E D
ED
D000483:Alleles; D000595:Amino Acid Sequence; D002460:Cell Line; D002648:Child; D005091:Exons; D005260:Female; D014644:Genetic Variation; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007438:Introns; D008297:Male; D011110:Polymorphism, Genetic; D017421:Sequence Analysis
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A