Relaxin favors the development of activated human T cells into Th1-like effectors.
Differentiation of naive CD4+ helper T (Th) cells into Th1 or Th2 effectors, as characterized by their opposite pattern of cytokine production, can be influenced by several factors, including hormones. In this study, we demonstrate that porcine relaxin, at concentrations ranging from 10(-10) to 10(-6) M, favors the in vitro development of human antigen-specific T cells into Th1-like effectors and enhances both IFN-gamma mRNA expression and IFN-gamma production by established human T cell clones. The promoting effect of relaxin on the development of IFN-gamma-producing cells was not due to a relaxin-induced release of IL-12 and/or IFN-alpha by antigen-presenting cells. These results suggest that relaxin may contribute to the regulation of the immune homeostasis during pregnancy and may also play some role in counteracting Th2-dominated disorders.
Piccinni
M P
MP
Department of Internal Medicine, University of Florence, Italy.
Bani
D
D
Beloni
L
L
Manuelli
C
C
Mavilia
C
C
Vocioni
F
F
Bigazzi
M
M
Sacchi
T B
TB
Romagnani
S
S
Maggi
E
E
D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012065:Relaxin / Q000494:pharmacology*; D013552:Swine; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
D017931:DNA Primers; D012333:RNA, Messenger; D013745:Tetanus Toxoid; D015847:Interleukin-4; D007371:Interferon-gamma; D012065:Relaxin