[Blood levels of homocysteine by high pressure liquid chromatography and comparison with two other techniques].
Cardio-vascular diseases are the most common cause of death in industrialized countries. A new marker has emerged among offending risk factors in the past few years: homocysteine. This sulphured amino-acid is an important intermediate in transsulphuration and remethylation reactions of methionine's metabolism. We proposed to evaluate a home made method of determination for this parameter by high performance liquid chromatography (HPLC) and to compare it to fluorescence polarization immunoassay technique (FPIA) and to gaz phase chromatography (CG-SM). This method associated with good sensibility and precision remain much less expensive than FPIA technique.
Ceppa
F
F
Laboratoire de biochimie, toxicologie et pharmacologie cliniques, Hôpital d'Instruction des Armées Begin, 69, avenue de Paris, 94163 Saint-Mandé cedex.
Drouillard
I
I
Chianea
D
D
Burnat
P
P
Perrier
F
F
Vaillant
C
C
El Jahiri
Y
Y
D000111:Acetylcysteine / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003545:Cysteine / Q000097:blood; D004151:Dipeptides / Q000097:blood; D005455:Fluorescent Antibody Technique; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005978:Glutathione / Q000097:blood; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity
D015415:Biomarkers; D004151:Dipeptides; D006710:Homocysteine; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine; D000111:Acetylcysteine
Dosage de l'homocysteéine plasmatique par chromatographie liquide haute performance et comparaison à deux autres techniques.