Gender determination from dental pulp by using capillary gel electrophoresis of amelogenin locus.
Gender of dental pulp DNA was determined by the capillary gel electrophoresis (CGE) method with amelogenin locus as a marker. The analysis of the male sample resulted in two peaks, a Y-locus standard sample was added and the CGE method repeated. This resulted in an amplification of the second peak and judging from the difference between the allele sizes of the X and Y loci, it was confirmed that the first peak was for the X and the second for the Y locus. The female sample was then analyzed and one peak was observed. As before the X-locus standard sample was added to this sample and resulted in an amplification of the peak, thus verifying it to be the X locus. The CGE method was conducted for 10 male and 10 female samples and double peaks verified the male sample, while one peak verified the female sample. Gender was thus correctly determined in all cases.
Komuro
T
T
Department of Legal Medicine, Nihon University School of Dentistry, Tokyo, Japan.
Nakamura
M
M
Tsutsumi
H
H
Mukoyama
R
R
D053523:Amelogenin; D003746:Dental Enamel Proteins / Q000235:genetics*; D003782:Dental Pulp / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005260:Female; D005553:Forensic Dentistry / Q000379:methods*; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis / Q000379:methods*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D053523:Amelogenin; D003746:Dental Enamel Proteins