Proteolytic activity in the yolk sac membrane of quail eggs.
Extraembryonal degradation of yolk protein is necessary to provide the avian embryo with required free amino acids during early embryogenesis. Screening of proteolytic activity in different compartments of quail eggs revealed an increasing activity in the yolk sac membrane during the first week of embryogenesis. In this tissue, the occurrence of cathepsin B, a lysosomal cysteine proteinase, and cathepsin D, a lysosomal aspartic proteinase, has been described recently (Gerhartz et al., Comp Biochem Physiol, 118B:159-166, 1997). Determination of cathepsin B-like and cathepsin D-like proteolytic activity in the yolk sac membrane indicated a significant correlation between growth of the yolk sac membrane and proteolytic activity, shown by an almost constant specific activity. Both proteinases could be localized in the endodermal cells, which are in direct contact to the yolk. The concentration of proteinases in the endodermal cells appears to be almost unaltered in the investigated early stage of quail development, whereas the amount of endodermal cells increases rapidly, seen by a complicated folding of the yolk sac membrane. In the same cells quail cystatin, a potent inhibitor of quail cathepsin B (Ki 0.6 nM), has been localized at day 8 of embryonic development. Approximately at this stage of development, the quail embryo stops metabolizing yolk. In conclusion, it is strongly indicated that the amount of available free amino acids, produced by proteolytic degradation and supporting embryonic growth, is regulated by the growth of the yolk sac membrane.
Gerhartz
B
B
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät der LMU München, Germany.
Kolb
H J
HJ
Wittmann
J
J
D000818:Animals; D002401:Cathepsin B / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D003370:Coturnix / Q000196:embryology* / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000502:physiology; D004707:Endoderm / Q000166:cytology / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism*; D014018:Tissue Distribution / Q000502:physiology; D015017:Yolk Sac / Q000166:cytology / Q000201:enzymology*
D010447:Peptide Hydrolases; D002401:Cathepsin B; D002402:Cathepsin D