Interleukin-8 stimulates the adhesion of endometrial stromal cells to fibronectin.
OBJECTIVE
To study the effect of interleukin-8 (IL-8) and mouse anti-human IL-8 monoclonal antibody on endometrial stromal cell adhesion to fibronectin.


METHODS
Prospective, controlled in vitro study.


METHODS
Academic research laboratory.


METHODS
Women undergoing benign gynecologic surgery for indications other than endometrial disease.


METHODS
Endometrial stromal cells were treated with IL-8 and mouse anti-human IL-8 monoclonal antibody at different concentrations and adhesion assays were performed by labeling the cells with calcein.


METHODS
Fluorescence in calcein-labeled endometrial stromal cells.


RESULTS
IL-8 induced a concentration-dependent increase of cell adhesion to fibronectin matrix (twofold at 10 ng/mL; P = .006). Treatment of endometrial stromal cells with anti-IL-8 neutralizing antibody inhibited partially the cell adhesion.


CONCLUSIONS
IL-8 stimulates the adhesion of endometrial stromal cells to fibronectin in a concentration-dependent manner. IL-8 may thus be relevant for the attachment of endometrial implants in the pathogenesis of endometriosis.
Garcia-Velasco
J A
JA
Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, Connecticut 06520-8063, USA.
Arici
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000166:cytology* / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology* / Q000502:physiology; D007700:Kinetics; D051379:Mice; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D016209:Interleukin-8