Effect of hydrogen peroxide on the metabolism of articular chondrocytes.
OBJECTIVE
To examine the effect of hydrogen peroxide on chondrocyte metabolism.


METHODS
Bovine articular chondrocytes were used. Proteoglycan (PG) synthesis was measured with [35S] sulfate incorporation. For detection of apoptosis, the TdT-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V assay were used. Extracellular-regulated protein kinase (ERK) activity was measured using a mitogen-activated protein kinase assay system.


RESULTS
Addition of hydrogen peroxide resulted in the inhibition of PG synthesis, apoptosis, and enhanced ERK activity.


CONCLUSIONS
Hydrogen peroxide plays an important role in regulating the metabolism of chondrocytes.
Asada
S
S
Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka-sayama, Osaka, Japan.
Fukuda
K
K
Oh
M
M
Hamanishi
C
C
Tanaka
S
S
D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D011509:Proteoglycans / Q000096:biosynthesis
D017304:Annexin A5; D011509:Proteoglycans; D006861:Hydrogen Peroxide; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases