A fully automated multicapillary electrophoresis device for DNA analysis.
We describe the construction and performance of a fully automated multicapillary electrophoresis system for the analysis of fluorescently labeled biomolecules. A special detection system allows the simultaneous spectral analysis of all 96 capillaries. The main features are true parallel detection without any moving parts, high robustness, and full compatibility to existing protocols. The device can process up to 40 microtiter plates (96 and 384 well) without human interference, which means up to 15,000 samples before it has to be reloaded.
Behr
S
S
Abteilung Lehrach, Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany.
Mätzig
M
M
Levin
A
A
Eickhoff
H
H
Heller
C
C
D001331:Automation; D010584:Bacteriophage phi X 174 / Q000235:genetics; D004247:DNA / Q000032:analysis*; D003627:Data Interpretation, Statistical; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D008027:Light; D012984:Software; D013997:Time Factors
D005456:Fluorescent Dyes; D004247:DNA