[Expression of reactive oxygen-related enzymes in human umbilical vein endothelial cells (HUVEC) cultured with high concentrations of glucose].
We examined the levels of reactive oxygen-related enzymes in human umbilical vein endothelial cells cultured with high concentrations of glucose, in vitro. From the results, elevated levels of catalase mRNA and its protein were exhibited in the presence of higher glucose. In addition, the message level of p22-phox as the active center of NADPH oxidase, was slightly increased. Taken together, the endothelial injury induced by diabetes may associated to the elevated level of O2- production. However, the level of catalase as .OH scavenger was mainly increased, cooperatively.
Nakano
A
A
Kanto Biomedical Laboratories, Kohnosu.
Koyama
I
I
Matsunaga
T
T
Nakajima
T
T
Hirose
H
H
Sato
M
M
Komoda
T
T
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology*; D016166:Free Radical Scavengers / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D006943:Hyperglycemia / Q000201:enzymology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014471:Umbilical Veins
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases; D005947:Glucose