Instability of chromosome 7 in colony forming cells of patients with aplastic anemia.
Monosomy 7 in isolated bone marrow mononuclear cells and colony forming cells from patients with aplastic anemia who later developed myelodysplastic syndrome/leukemia with monosomy 7 were examined by fluorescence in situ hybridization. Colonies derived from bone marrow mononuclear cells of the aplastic anemia patients consisted of a mixture of cells of normal karyotype and monosomy 7, ranging from 0 to 97.2%. This result suggests that colony forming cells initially had a normal karyotype but then lost chromosome 7 during growth in the semisolid culture. This finding suggests the genetically unstable condition of chromosome 7 in colony forming cells in aplastic anemia patients. This chromosome instability of colony forming cells may lead to malignant transformation.
Ueda
H
H
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Tashiro
S
S
Kojima
S
S
Tanaka
K
K
Eguchi
M
M
Ueda
K
K
Kamada
N
N
D000741:Anemia, Aplastic / Q000235:genetics*; D001854:Bone Marrow Cells; D002648:Child; D002869:Chromosome Aberrations / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7; D006412:Hematopoietic Stem Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009006:Monosomy