Structural organization of the 5'-end and chromosomal assignment of human placental leucine aminopeptidase/insulin-regulated membrane aminopeptidase gene.
Placental leucine aminopeptidase (P-LAP) which is identical with cystine aminopeptidase as oxytocinase was found to be a homologue of rat insulin-regulated membrane aminopeptidase (IRAP) by cDNA cloning. In this study, we confirmed 5'-end cDNA sequence of P-LAP and isolated genomic clones containing the upstream region of human P-LAP gene. The transcription initiation sites determined by primer extension located 478 and 480 bp upstream of the initiation methionine codon, 38 bp downstream of TATA box-like motif. The 5'-flanking region of human P-LAP gene contained DNA-binding motifs for several ubiquitous transcription factors such as SP1 and AP2. Chromosomal localization by fluorescence in situ hybridization showed that the gene was assigned to 5q14.2-q15 of the human chromosome. This study establishes the genetic basis for P-LAP gene research, thereby leading to better understanding of the molecular mechanism underlying the P-LAP gene.
Horio
J
J
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya, 466-8550, Japan.
Nomura
S
S
Okada
M
M
Katsumata
Y
Y
Nakanishi
Y
Y
Kumano
Y
Y
Takami
S
S
Kinoshita
M
M
Tsujimoto
M
M
Nakazato
H
H
Mizutani
S
S
D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D010122:Cystinyl Aminopeptidase / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D010920:Placenta / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D020218:Response Elements / Q000235:genetics; D016415:Sequence Alignment; D016385:TATA Box / Q000235:genetics
D018387:Codon, Initiator; D012333:RNA, Messenger; D000626:Aminopeptidases; D010122:Cystinyl Aminopeptidase; C105964:leucyl-cystinyl aminopeptidase