Coordinate regulation of RAG1 and RAG2 by cell type-specific DNA elements 5' of RAG2.
RAG1 and RAG2 are essential for V(D)J recombination and lymphocyte development. These genes are thought to encode a transposase derived from a mobile genetic element that was inserted into the vertebrate genome 450 million years ago. The regulation of RAG1 and RAG2 was investigated in vivo with bacterial artificial chromosome (BAC) transgenes containing a fluorescent indicator. Coordinate expression of RAG1 and RAG2 in B and T cells was found to be regulated by distinct genetic elements found on the 5' side of the RAG2 gene. This observation suggests a mechanism by which asymmetrically disposed cis DNA elements could influence the expression of the primordial transposon and thereby capture RAGs for vertebrate evolution.
Yu
W
W
Laboratory of Molecular Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Misulovin
Z
Z
Suh
H
H
Hardy
R R
RR
Jankovic
M
M
Yannoutsos
N
N
Nussenzweig
M C
MC
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D016654:Genes, RAG-1; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011993:Recombinant Fusion Proteins; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D013601:T-Lymphocytes / Q000378:metabolism*; D014158:Transcription, Genetic; D019076:Transgenes; D019895:Transposases / Q000235:genetics
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; C499308:Rag2 protein, mouse; D011993:Recombinant Fusion Proteins; C064655:V(D)J recombination activating protein 2; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D019895:Transposases