The detection of human beta 2-microglobulin by grating coupler immunosensor with three dimensional antibody networks.
Immunosensors for the detection of human beta 2-microglobulin (B2M) were prepared by immobilisation of covalently crosslinked assemblies containing various numbers of molecular layers of monoclonal antibody against B2M (anti-B2M) on the surface of a Ta2O5 grating coupler sensor. The immobilisation procedure consisted of repeated successive adsorption of anti-B2M and dextran sulfate (DS) followed by glutaraldehyde (GA) crosslinking of anti-B2M and washing out DS. The flexibility of the resulting anti-B2M networks was evaluated from the sensor response to the reversible expansion and contraction of the networks induced by changing pH of the ambient solution. A decreased GA concentration and the use of a higher-molecular-mass DS increased the network flexibility. The sensor sensitivity to B2M increased with increasing flexibility of the antibody networks and with increasing number of anti-B2M molecular layers, indicating that B2M can penetrate inside the antibody network.
Brynda
E
E
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. office@imc.cas.cz
Houska
M
M
Brandenburg
A
A
Wikerstal
A
A
Skvor
J
J
D000327:Adsorption; D000906:Antibodies / Q000276:immunology*; D015374:Biosensing Techniques; D003432:Cross-Linking Reagents; D005976:Glutaral; D006801:Humans; D012680:Sensitivity and Specificity; D001613:beta 2-Microglobulin / Q000032:analysis*
D000906:Antibodies; D003432:Cross-Linking Reagents; D001613:beta 2-Microglobulin; D005976:Glutaral