title: The accentuating effect of low magnesium concentration on cholestane-3 beta, 5 alpha, 6 beta-triol-induced decrease of LDL uptake by cultured endothelial cells.
abstract: The effects of cholestane-3 beta, 5 alpha, 6 beta-triol (TriolC) and/or low magnesium on uptake, internalization, surface binding and degradation of 125I-LDL were investigated in cultured endothelial cells. TriolC (at a level of 1 microgram/ml and higher) or magnesium (at a level of 188 microM and lower) in cultured medium decreased significantly the uptake, internalization, surface binding and degradation of 125I-LDL. When TriolC and low magnesium were combined, the significant inhibitions of uptake, internalization, surface binding and degradation of 125I-LDL were also observed although by themselves at these levels (TriolC lower than 1 microgram/ml and magnesium higher than 188 microM) they had no obvious effects. Our work suggests that a combination of low magnesium and TriolC have additive inhibitive effects on uptake, internalization, surface binding and degradation of 125I-LDL.
authors.affiliation: Harlan E. Moore Heart Research Foundation, Champaign, IL 61820, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: Hulea
authors.forename: F A
authors.initials: FA
authors.lastname: Kummerow
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002777:Cholestanols / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000960:Hypolipidemic Agents / Q000378:metabolism / Q000494:pharmacology*; D007457:Iodine Radioisotopes / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism / Q000493:pharmacokinetics*; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D002777:Cholestanols; D000960:Hypolipidemic Agents; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; C000474:cholestane-3,5,6-triol; D008274:Magnesium