title: Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro.
abstract: BACKGROUND
  Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system.
  
  
  METHODS
  Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA.
  
  
  RESULTS
  After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus.
  
  
  CONCLUSIONS
  This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.
authors.affiliation: Howard Hughes Medical Institute, University of Michigan Medical Center, Department of Internal Medicine and Biological Chemistry, Ann Arbor 48109-0650, USA.
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: C
authors.initials: C
authors.lastname: Barker
authors.forename: X
authors.initials: X
authors.lastname: Danthinne
authors.forename: M J
authors.initials: MJ
authors.lastname: Imperiale
authors.forename: G J
authors.initials: GJ
authors.lastname: Nabel
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D002478:Cells, Cultured; D003360:Cosmids; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D019426:Integrases / Q000378:metabolism*; D007763:Lac Operon; D009130:Muscle, Smooth / Q000166:cytology; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D013552:Swine; D014162:Transfection; D014764:Viral Proteins
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases