title: Heat-induced degradation of PER and TIM in Drosophila bearing a conditional allele of the heat shock transcription factor gene.
abstract: Heat pulses elicit dramatic and rapid decreases in the levels of the D. melanogaster period (per) and timeless (tim) proteins (i.e., PER and TIM). To investigate the possible role of the heat shock pathway in this response, we used Drosophila bearing a conditional allele of the hsf gene (termed hsf4), which encodes the heat shock transcription factor (HSF). At all times in a daily cycle, heat-induced decreases in the levels of PER and TIM were similar in wild-type and hsf4 mutant flies. The results strongly suggest that the heat shock pathway contributes little, if any, to the response of the Drosophila circadian clock to heat signals.
authors.affiliation: Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, USA.
authors.forename: D
authors.initials: D
authors.lastname: Sidote
authors.forename: I
authors.initials: I
authors.lastname: Edery
mesh_terms: D000483:Alleles; D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D017344:Genes, Insect; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics* / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; C053134:Hsf protein, Drosophila; D019476:Insect Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D014157:Transcription Factors; C096445:tim protein, Drosophila