Effect of endotoxin on protein degradation and lipid peroxidation of erythrocytes.
Sepsis has often been associated with infection due to endotoxin (LPS) produced from gram-negative bacteria. Microcirculatory failure is one of the ultimate causes of septic shock. We studied the effect of endotoxin on the protein breakdown and lipid peroxidation of erythrocyte. In vivo (20 ug LPS/100 g) studies in rats showed increased tyrosine production from erythrocyte, as an index of protein degradation in erythrocyte. In vitro studies using 25 microg to 250 microg LPS per ml also showed similar type of increased effect of endotoxin in protein degradation. Washed erythrocyte devoid of plasma and leucocytes did not show any increased effect after endotoxin treatment. Lipid peroxidation was also increased after endotoxin treatment. However, protein degradation was more prominent than lipid peroxidation. We concluded therefore that the protein degradation and lipid peroxidation of erythrocytes caused by endotoxin are probably related to the production of septic shock.
Bhattacharyya
J
J
Indian Institute of Chemical Biology, Jadavpur, Calcutta.
Chowdhury
T D
TD
Datta
A G
AG
D000818:Animals; D004731:Endotoxins / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D004926:Escherichia coli; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D011489:Protein Denaturation / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014443:Tyrosine / Q000097:blood
D004731:Endotoxins; D008070:Lipopolysaccharides; D014443:Tyrosine