title: Analysis of p53 gene mutations in Helicobacter pylori-associated gastritis mucosa in endoscopic biopsy specimens.
abstract: BACKGROUND
  No previous report has shown the relationship between Helicobacter pylori infection and a direct sequence analysis of p53 gene mutation in a biopsy sample of human gastric mucosa.
  
  
  METHODS
  A total of 60 endoscopic biopsies samples (21 patients with H. pylori-positive gastritis and 9 patients with H. pylori-negative gastritis), including antral mucosa and corpus mucosa, were used in this study. Direct DNA sequencing of exons 5, 6, 7, and 8 of the p53 gene was performed by the dyedeoxy terminator method.
  
  
  RESULTS
  Mutations in the p53 gene were identified in non-hot spot codons in exon 7 and 8 in 11 of 21 samples (52.4%) from H. pylori-positive gastritis patients. There was no mutation in H. pylori-negative gastritis patients.
  
  
  CONCLUSIONS
  This finding shows that H. pylori infection can induce p53 point mutations and appears to be involved in the pathway leading to dysplasia or carcinoma.
authors.affiliation: Second Dept. of Internal Medicine, Oita Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Fujioka
authors.forename: T
authors.initials: T
authors.lastname: Okimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Mitsuishi
authors.forename: T
authors.initials: T
authors.lastname: Oda
authors.forename: A
authors.initials: A
authors.lastname: Nishizono
authors.forename: M
authors.initials: M
authors.lastname: Nasu
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D005756:Gastritis / Q000382:microbiology / Q000473:pathology*; D016158:Genes, p53 / Q000235:genetics*; D016481:Helicobacter Infections / Q000235:genetics* / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation