Prestorage and bedside leucofiltration of whole blood modulates storage-time-dependent suppression of in vitro TNFalpha release.
Immunosuppression after transfusion may be related to the content of leucocytes in the transfused blood. Therefore we studied the effects of prestorage and bedside leucodepletion by filtration on the suppression by whole blood of in vitro stimulated tumour necrosis factor alpha (TNFalpha) release. Nine units of whole blood were leucofiltered prestorage and stored for 35 d. 27 units, 3 x 9, were stored and leucofiltered at the bedside after 7, 21 and 35 d. Supernatants were collected from all units during storage and added to a whole blood assay of E. coli-LPS-stimulated TNFalpha release. The effects of storage were assessed and compared with supernatants collected immediately after donation as reference. TNFalpha release was storage time dependently suppressed to: 81%, 74% and 57% by supernatants from non-filtered blood stored for 7, 21 and 35 d, respectively. Prestorage leucofiltration almost eliminated this effect, but we still observed a storage-time-dependent suppression by bedside-leucofiltered blood to 88%, 78% and 65%, respectively. Prestorage leucofiltration appeared to reduce storage-time-dependent suppression of in vitro stimulated TNFalpha release induced by plasma from whole blood compared with non-filtered and bedside-leucofiltered whole blood.
Mynster
T
T
Surgical Immunology Laboratory and Department of Surgical Gastroenterology, Hvidovre University Hospital, Copenhagen, Denmark. mynster@dadlnet.dk
Hammer
J H
JH
Nielsen
H J
HJ
D001769:Blood; D001781:Blood Component Removal / Q000379:methods*; D001803:Blood Transfusion / Q000379:methods; D005374:Filtration; D007165:Immunosuppression Therapy; D007962:Leukocytes; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha