Impaired recoloration of a discordant liver xenograft in the guinea pig-to-rat combination: physiological insults or immunological responses.
BACKGROUND
The aim of this study was to clarify, using guinea pig (GP) syngeneic and xenogeneic liver graft models, the mechanisms of impaired recoloration caused by immunological responses or physiological insults; and to examine the effect of an anti-complement agent, FC43 emulsion, on xenograft rejection.


RESULTS
GP syngeneic and xenogeneic liver grafts flushed with 4 degrees C lactated Ringer's solution were poorly perfused around the porta hepatis, whereas those flushed with 15 degrees C solution were immediately recolored, and the portal venography confirmed homogeneous perfusion throughout the GP livers. Impaired recoloration of GP xenografts was also ameliorated by reduction of ischemic time. Pretreatments with FC43 emulsion significantly extended xenograft survivals. Rejected GP liver xenografts showed C3 deposits on sinusoids and central veins and IgM deposits faintly stained only on some small vessels, but there were no detectable deposits of IgG.


CONCLUSIONS
The impaired recoloration in this model may be caused by physiological insults during the initial stage of revascularization, and the immunological responses that probably followed.
Tanaka
M
M
Department of Surgery, Research Institute for Artificial Organs, Transplantation and Gene Therapy, Sapporo Hokuyu Hospital, Japan. m-tanaka@qc4.so-net.ne.jp
Tamaki
T
T
Konoeda
Y
Y
Kawamura
A
A
Takahashi
Y
Y
Ishikura
H
H
Yoshiki
T
T
D000208:Acute Disease; D000818:Animals; D003080:Cold Temperature; D003169:Complement Inactivator Proteins / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology; D006084:Graft Rejection / Q000503:physiopathology; D006168:Guinea Pigs; D016031:Liver Transplantation / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000209:etiology; D014183:Transplantation, Heterologous / Q000502:physiology
D003169:Complement Inactivator Proteins; D005466:Fluorocarbons