title: Molecular analysis of the gonadotropin-releasing hormone receptor in patients with polycystic ovary syndrome.
abstract: OBJECTIVE
  To determine whether a mutation in the GnRH receptor gene is responsible for polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Molecular analysis of human genomic DNA.
  
  
  METHODS
  Academic research environment.
  
  
  METHODS
  Eighty patients with PCOS.
  
  
  METHODS
  Extraction and polymerase chain reaction (PCR) analysis of genomic DNA, confirmation of PCR products by ethidium bromide staining of agarose gels after electrophoresis, denaturing gradient gel electrophoresis of PCR products, and photography.
  
  
  METHODS
  Mutations in the GnRH receptor of women with PCOS.
  
  
  RESULTS
  Denaturing gradient gel electrophoresis revealed no mutations in the exonic sequence encoding the open reading frame of the GnRH receptor.
  
  
  CONCLUSIONS
  A mutation in the GnRH receptor gene is unlikely to be the underlying cause of PCOS in most patients. The molecular basis of this disorder remains unknown.
authors.affiliation: University of Chicago Hospital, Pritzker Medical School, Illinois 60637, USA. dcohen@babies.bsd.uchicago.edu
authors.forename: D P
authors.initials: DP
authors.lastname: Cohen
authors.forename: E M
authors.initials: EM
authors.lastname: Stein
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: C K
authors.initials: CK
authors.lastname: Matulis
authors.forename: D A
authors.initials: DA
authors.lastname: Ehrmann
authors.forename: L C
authors.initials: LC
authors.lastname: Layman
mesh_terms: D000293:Adolescent; D000328:Adult; D004252:DNA Mutational Analysis; D005091:Exons; D005260:Female; D006801:Humans; D016366:Open Reading Frames; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011966:Receptors, LHRH / Q000235:genetics*
chemical_list: D011966:Receptors, LHRH