title: Intrinsic myenteric denervation: a new model to increase the intestinal absorptive surface in short-bowel syndrome.
abstract: BACKGROUND
  Short-bowel syndrome (SBS) is caused by resection of massive portions of the small intestine and is characterized by symptoms related to malabsorption, of which severe weight loss is the most apparent. Surgical treatments for SBS are not yet satisfactory. In rats, the myenteric denervation by benzalkonium chloride (BAC) leads to development of megaileum with visceral dilatation and mucosal hyperplasia and increases the intestinal transit time. Such operation in the remaining intestinal segment after massive small bowel resection could increase the duration of contact between luminal nutrients and ileal mucosal epithelium, and furthermore, it could increase the superficial area of the mucosa. Thus, our aim in this study was to evaluate the epithelial morphology and body weight changes of animals after intrinsic ileal denervation associated with extensive small intestine resection.
  
  
  METHODS
  Wistar rats were submitted to resection of 80% of small intestinal length (Group R). Another group (B) of animals also received topical serosal application of BAC 0.3%. Control animals were submitted to simulated surgery (Group C). Animals were weighed weekly and sacrificed after 90 days. Intestinal walls were collected for histological procedure and morphometry.
  
  
  RESULTS
  At the end of the experimental period all groups showed weight increase, which was reduced in the R group (P < 0.01). Interestingly, the denervated Group B showed a marked increase in weight, similar to the control animals. Morphometric analysis of the mucosal layer area showed a major increase in mucosal surface area, mainly in Group B.
  
  
  CONCLUSIONS
  Our results showed that the ileal intrinsic denervation associated with massive intestinal resection induced an increase in the superficial absorptive area and was able to improve the postsurgical conditions for the animals, with accentuated weight increase. This procedure may be a useful model for further studies related to the role of the enteric nervous system on intestinal adaptations after extensive resections and may provide a new approach for the surgical treatment of short-bowel syndrome.
authors.affiliation: Department of Morphology, University of Sao Paulo, Brazil.
authors.forename: S B
authors.initials: SB
authors.lastname: Garcia
authors.forename: M C
authors.initials: MC
authors.lastname: Kawasaky
authors.forename: J C
authors.initials: JC
authors.lastname: Silva
authors.forename: A C
authors.initials: AC
authors.lastname: Garcia-Rodrigues
authors.forename: T J
authors.initials: TJ
authors.lastname: Borelli-Bovo
authors.forename: A C
authors.initials: AC
authors.lastname: Iglesias
authors.forename: S
authors.initials: S
authors.lastname: Zucoloto
mesh_terms: D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D003714:Denervation; D017615:Enteric Nervous System / Q000187:drug effects; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D007082:Ileum / Q000187:drug effects / Q000294:innervation / Q000473:pathology; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D008286:Malabsorption Syndromes / Q000209:etiology / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000150:complications / Q000503:physiopathology*
chemical_list: D001548:Benzalkonium Compounds