title: Isolation and characterization of microsatellite loci in black rhinoceros (Diceros bicornis).
abstract: The black rhinoceros (Diceros bicornis) is currently one of the most endangered African mammals as a direct result o habitat destruction and intensive poaching. A microsatellite library was constructed to address a number of population genetic questions. This paper describes the isolation of five black rhinoceros microsatellite loci. Three of these loci were found to be polymorphic. In addition, the paper demonstrates the utility for cross-hybridization of these primers in other species of rhinoceros.
authors.affiliation: Department of Biochemistry, University of Cape Town, Rondebosch, South Africa.
authors.forename: J
authors.initials: J
authors.lastname: Cunningham
authors.forename: E H
authors.initials: EH
authors.lastname: Harley
authors.forename: C
authors.initials: C
authors.lastname: O'Ryan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D018895:Microsatellite Repeats / Q000235:genetics*; D010527:Perissodactyla / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers