title: Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050.
abstract: A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of approximately 80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.
authors.affiliation: Division of Biochemistry and Molecular Biology, IBLS, University of Glasgow, United Kingdom.
authors.forename: N J
authors.initials: NJ
authors.lastname: Fraser
authors.forename: P J
authors.initials: PJ
authors.lastname: Dominy
authors.forename: B
authors.initials: B
authors.lastname: Ucker
authors.forename: I
authors.initials: I
authors.lastname: Simonin
authors.forename: H
authors.initials: H
authors.lastname: Scheer
authors.forename: R J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D002021:Buffers; D002942:Circular Dichroism; D003902:Detergents; D004735:Energy Transfer; D005960:Glucosides; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D012241:Rhodopseudomonas / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D002021:Buffers; D003902:Detergents; D005960:Glucosides; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C023693:protein complex B800-850, Rhodobacter; C040358:dodecyl maltoside