title: The simultaneous measurement of epithelial ion transport and intracellular free Ca2+ in cultured equine sweat gland secretory epithelium.
abstract: We explored the relationship between nucleotide-evoked changes in intracellular free calcium ([Ca2+]i) and anion secretion by measuring [Ca2+]i and I(SC) simultaneously in Fura-2-loaded, cultured equine sweat gland epithelia. Apical ATP, UTP or UDP elicited sustained increases in [Ca2+]i that were initiated by the mobilization of cytoplasmic Ca2+ but maintained by Ca2+ influx. However, although these nucleotides also increased I(SC), this response was transient whereas the [Ca2+]i signals were sustained. Experiments in which external Ca2+ was removed/replaced showed that Ca2+ entering nucleotide-stimulated cells elicited very little change in ISC. Cross desensitization experiments showed that UTP-stimulated epithelia became insensitive to ATP but that UTP could increase both [Ca2+]i and ISC in ATP-stimulated cells by activating 'pyrimidinoceptors' essentially insensitive to ATP. Thapsigargin evoked a sustained rise in [Ca2+]i that was accompanied by a maintained increase in ISC. However, this increase in ISC was dependent upon external Ca2+ and so the responses to nucleotides and thapsigargin have different properties. ATP increased ISC in thapsigargin-treated cells without causing any rise in [Ca2+]i while ionomycin increased both parameters. The data therefore show that apical P2Y receptors allow nucleotides to increase ISC via two mechanisms, one of which appears to be [Ca2+]i-independent control of anion channels.
authors.affiliation: Department of Physiology, The Chinese University of Hong Kong, New Territories, Hong Kong, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Ko
authors.forename: V W
authors.initials: VW
authors.lastname: Law
authors.forename: H Y
authors.initials: HY
authors.lastname: Wong
authors.forename: S M
authors.initials: SM
authors.lastname: Wilson
mesh_terms: D000818:Animals; D000838:Anions; D002118:Calcium / Q000032:analysis / Q000502:physiology; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002273:Carcinogens / Q000494:pharmacology; D002461:Cell Line, Transformed; D002614:Chelating Agents / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D006736:Horses; D007425:Intracellular Membranes / Q000737:chemistry*; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology*; D009711:Nucleotides / Q000494:pharmacology; D013545:Sweat Glands / Q000166:cytology; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D000838:Anions; D002273:Carcinogens; D002614:Chelating Agents; D009711:Nucleotides; D004533:Egtazic Acid; D019284:Thapsigargin; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium