Synthesis of new fluorescent nucleoside analogues and application to the study of human deoxycytidine kinase.
We have determined the affinity of human deoxycytidine kinase with respect to new fluorescent N-methylanthraniloyl cytidine derivatives or non fluorescent enantiomeric cytidine analogues. New results regarding the enantioselectivity and the mechanism of the enzyme are presented.
Shafiee
M
M
Laboratoire de Chimie Bioorganique, UMR 5625 du CNRS, Université Montpellier II, France.
Gosselin
G
G
Imbach
J L
JL
Eriksson
S
S
Maury
G
G
D003842:Deoxycytidine Kinase / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D009705:Nucleosides / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009705:Nucleosides; D003842:Deoxycytidine Kinase