title: A comparison of in vitro tests and a faecal egg count reduction test in detecting anthelmintic resistance in horse strongyles.
abstract: This study reports a comparison between faecal egg count reduction test (FECRT), egg hatch assay (EHA) and larval development assay (LDA) for detecting anthelmintic resistance in equine strongyles. Resistance to benzimidazoles was demonstrated in 33 of 42 (79%) farms tested by FECRT and in 32 (62%) of the 52 farms tested by EHA. As the reference strain used was not fully susceptible to benzimidazoles it was not possible to determine the level of resistance by LDA. Pyrantel resistance was indicated on three of 15 farms by faecal egg count reduction. Resistance was also indicated by LDA for one of these farms. In addition resistance was indicated by LDA on two more farms that were not tested by FECRT. Further testing is needed to confirm if these findings are truly indicative of resistance. Generally, correlations between the tests were poor and it was not possible to use the outcome of one test to predict the outcome of another.
authors.affiliation: Danish Veterinary Laboratory, Copenhagen. jcr@kvl.dk
authors.forename: J
authors.initials: J
authors.lastname: Craven
authors.forename: H
authors.initials: H
authors.lastname: Bjørn
authors.forename: E H
authors.initials: EH
authors.lastname: Barnes
authors.forename: S A
authors.initials: SA
authors.lastname: Henriksen
authors.forename: P
authors.initials: P
authors.lastname: Nansen
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology / Q000627:therapeutic use*; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D006734:Horse Diseases / Q000188:drug therapy* / Q000469:parasitology; D006736:Horses; D007559:Ivermectin / Q000494:pharmacology / Q000627:therapeutic use; D007978:Levamisole / Q000494:pharmacology / Q000627:therapeutic use; D008463:Mebendazole / Q000494:pharmacology / Q000627:therapeutic use; D010270:Parasite Egg Count / Q000662:veterinary; D011715:Pyrantel / Q000494:pharmacology / Q000627:therapeutic use; D017206:Strongylida Infections / Q000188:drug therapy*; D013323:Strongylus / Q000187:drug effects*; D011795:Surveys and Questionnaires; D013827:Thiabendazole / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000871:Anthelmintics; D007978:Levamisole; D011715:Pyrantel; D007559:Ivermectin; D008463:Mebendazole; D013827:Thiabendazole