Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission.
A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)g7 haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established.
Nagafuchi
S
S
First Department of Internal Medicine, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. nagafuchi@shs.kyushu-u.ac.jp
Katsuta
H
H
Kogawa
K
K
Akashi
T
T
Kondo
S
S
Sakai
Y
Y
Tsukiyama
T
T
Kitamura
D
D
Niho
Y
Y
Watanabe
T
T
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D017931:DNA Primers; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D013234:Stem Cells / Q000166:cytology*
D017931:DNA Primers