Short exposure of polymorphonuclear leucocytes to sodium fluoride suppresses the response to fMLP.
Fluoridated dental care products are used to prevent dental decay. Up to now, there are no data available on whether the fluoride (F-) component of these products affects the bactericidal activity of salivary polymorpho-nuclear leucocytes, which are involved in the protection of the oral mucosa against infection. Therefore, after determining the concentration/time profile of F- in mixed saliva of healthy subjects after topical application of 0.5 g of a 1.25% F- containing gel, unstimulated and fMLP-stimulated polymorphonuclear leucocytes (PMNs) were shortly exposed to these F- concentrations and the generation of superoxide and hypochloric acid were measured, as well as the liberation of lysomal enzymes, and correlated with the cellular Ca2+ and cAMP-levels. The results show that F-, at concentrations as retained in saliva, did not activate the oxidative burst in unstimulated PMNs. In fMLP-activated PMNs, F-suppressed the receptor-mediated increase in the oxidative burst and the liberation of fl-glucuronidase by reduction of the availability of extracellular Ca2+ and, thus, the influx of Ca2+ necessary to couple completely the fMLP signal to effector pathways. These F- concentrations neither altered the liberation of Ca2+ from internal stores nor induced a rise in cAMP. The possible clinical consequences of these results for xerostomic patients with respect to the generation of HOSCN/OSCN/SCN in saliva an important non-immune factor for oral health, are dicussed.
Knoll-Köhler
E
E
Institute of Pharmacology, Medical Centre Benjamin Franklin Free University of Berlin, Germany.
Büssem
G
G
D000328:Adult; D002118:Calcium / Q000097:blood; D000242:Cyclic AMP / Q000097:blood; D005089:Exocytosis; D005260:Female; D006801:Humans; D006997:Hypochlorous Acid / Q000097:blood / Q000737:chemistry; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D016897:Respiratory Burst; D012463:Saliva / Q000737:chemistry; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood; D013654:Taurine / Q000737:chemistry
D013481:Superoxides; D013654:Taurine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006997:Hypochlorous Acid; D012969:Sodium Fluoride; D000242:Cyclic AMP; D002118:Calcium