[The relationship between the prognosis of retinoblastoma and proliferating cell nuclear antigen and argyrophilic protein at nucleolus organizer regions].
OBJECTIVE
To investigate the relationship between the proliferative activity of retinoblastoma (Rb) cells and the prognosis of the tumor.


METHODS
Proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizer regions (AgNORs) in paraffin-embedded Rb specimens from 48 cases were detected by LSAB immunohistochemical method using an anti-PCNA monoclonal antibody and the technique of ploton.


RESULTS
The PCNA labeling index (PCNALI) and the AgNORs counts were well correlated (r = 0.647, P < 0.001). In 29 patients of the 48 cases receiving simple eyeball extirpation and being followed up for 5 years, the mean PCNALI and the mean numbers of AgNORs per nucleus in 19 patients surviving > or = 5 years after operation were markedly lower than that in 10 patients surviving < 5 years after operation (P < 0.01, P < 0.05).


CONCLUSIONS
It is indicated that the PCNALI or the AgNOR count is negatively related with the clinical prognosis of Rb patients, and can be useful in judging the prognosis.
Li
D
D
Department of Ophthalmology, First Affiliated Hospital, Henan Medical University, Zhengzhou.
Song
X
X
Gao
Y
Y
D015353:Eye Enucleation; D005500:Follow-Up Studies; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D019572:Retinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D012175:Retinoblastoma / Q000378:metabolism* / Q000473:pathology; D016622:Silver Staining
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen