Confirmation of N-(nitrosomethyl) urea as a nitrosourea derived by nitrosation of fish sauce.
N-(nitrosomethyl) urea (NMU) was characterized in the carcinogenic nitrosated fish sauce (NFS) with high performance liquid chromatography (HPLC)-photohydrolysis-pyrolysis-thermal energy analysis recently. We used HPLC-electronic spray ionization-mass spectrometry and HPLC-diode array detection to confirm NMU in NFS further. It was observed that the corresponding chromatographic fraction of NMU of NFS showed the same mass spectrum (m/z 64, 102, and 145) and ultraviolet-absorbance (lambda max = 230 nm) as those of authentic NMU. These results confirmed that the component of NFS was NMU.
Deng
D J
DJ
Beijing Institute for Cancer Research & Beijing Medical University School of Oncology, China.
Yang
S M
SM
Li
T
T
Xin
H J
HJ
D002273:Carcinogens / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D005396:Fish Products / Q000032:analysis*; D013058:Mass Spectrometry; D008770:Methylnitrosourea / Q000138:chemical synthesis*; D013056:Spectrophotometry, Ultraviolet
D002273:Carcinogens; D008770:Methylnitrosourea