Assay for nucleotide excision repair protein activity using fractionated cell extracts and UV-damaged plasmid DNA.
Biggerstaff
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M
Clare Hall Laboratories, Imperial Cancer Research Fund, Potters Bar, UK.
Wood
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RD
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D011506:Proteins / Q000378:metabolism; D014020:Tissue Extracts / Q000378:metabolism; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011506:Proteins; D014020:Tissue Extracts; C095888:xeroderma pigmentosum group F protein; C071447:ERCC1 protein, human; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer); C106403:NTH protein, E coli