The cell wall of Fusarium oxysporum.
Sugar analysis of isolated cell walls from three formae speciales of Fusarium oxysporum showed that they contained not only glucose and (N-acetyl)-glucosamine, but also mannose, galactose, and uronic acids, presumably originating from cell wall glycoproteins. Cell wall glycoproteins accounted for 50-60% of the total mass of the wall. X-ray diffraction studies showed the presence of alpha-1, 3-glucan in the alkali-soluble cell wall fraction and of beta-1, 3-glucan and chitin in the alkali-insoluble fraction. Electron microscopy and lectin binding studies indicated that glycoproteins form an external layer covering an inner layer composed of chitin and glucan.
Schoffelmeer
E A
EA
Institute of Molecular Cell Biology, BioCentrum Amsterdam, Kruislaan 318, Amsterdam, 1098 SM, The Netherlands. schoffelmeer@bio.uva.nl
Klis
F M
FM
Sietsma
J H
JH
Cornelissen
B J
BJ
D002241:Carbohydrates / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D005656:Fungal Proteins / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry* / Q000648:ultrastructure; D005936:Glucans / Q000032:analysis; D008854:Microscopy, Electron
D002241:Carbohydrates; D005656:Fungal Proteins; D005936:Glucans