A locus for an axonal form of autosomal recessive Charcot-Marie-Tooth disease maps to chromosome 1q21.2-q21.3.
Charcot-Marie-Tooth disease (CMT) is a heterogeneous group of disorders that affect the peripheral nervous system. Three loci are known for the autosomal dominant forms of axonal CMT (CMT2), but none have yet been identified for autosomal recessive axonal CMT (ARCMT2). We have studied a large consanguineous Moroccan ARCMT2 family with nine affected sibs. The onset of CMT was in the 2d decade in all affected individuals who presented with a severe motor and sensory neuropathy, with proximal muscle involvement occurring in some patients. After exclusion of known loci for CMT2 and for demyelinating ARCMT2, a genomewide search was performed. Evidence for linkage was found with markers on chromosome 1q. The maximum pairwise LOD score was above the threshold value of 3.00, for markers D1S514, D1S2715, D1S2777, and D1S2721, and it reached 6.10 at the loci D1S2777, D1S2721, and D1S2624, according to multipoint LOD-score analysis. These markers defined a region of homozygosity that placed the gene in a 4.4-cM interval. Moreover, a recombination event detected in an unaffected 48-year-old individual excludes the D1S506 marker, thereby reducing the interval to 1.7 cM. In addition, the P0 gene, an attractive candidate because of both its location on chromosome 1q and its role in myelin structure, was excluded by physical mapping and direct sequencing.
Bouhouche
A
A
INSERM U.289, Fédération de Neurologie, Hôpital de la Salpêtrière, Bâtiment Nouvelle Pharmacie, Paris, France. abouhouche@hotmail.com
Benomar
A
A
Birouk
N
N
Mularoni
A
A
Meggouh
F
F
Tassin
J
J
Grid
D
D
Vandenberghe
A
A
Yahyaoui
M
M
Chkili
T
T
Brice
A
A
LeGuern
E
E
D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D001369:Axons / Q000473:pathology* / Q000502:physiology; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D002648:Child; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D003241:Consanguinity; D005260:Female; D005808:Genes, Recessive; D005819:Genetic Markers; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D008126:Lod Score; D008297:Male; D008875:Middle Aged; D009018:Morocco / Q000208:ethnology; D018993:Myelin P0 Protein / Q000235:genetics; D010375:Pedigree; D011995:Recombination, Genetic
D005819:Genetic Markers; D018993:Myelin P0 Protein