Multi-residue analysis of penicillin residues in porcine tissue using matrix solid phase dispersion.
The aim of this study was to develop a multi-residue method for the analysis of penicillins in animal tissue. Matrix solid phase dispersion (MSPD) was employed to extract the residues and the extracts were then cleaned-up by C18 solid phase extraction (SPE). Pre-column derivatisation using acetic anhydride and 1,2,4-triazole in the presence of mercuric chloride was employed to allow detection in 325 nm. Gradient elution was required to elute amoxicillin, ampicillin, penicillin G, cloxacillin and dicloxacillin derivatives from a C18 reversed phase column using phosphate buffer-acetonitrile mobile phase. The developed method had a limit of detection of 20 ng g-1 and had recoveries in the range 40-90% for the 5 drugs in samples fortified at 40 and 200 ng g-1; the maximum residue limits (MRLs) for these drugs were in the range of 50-300 ng g-1 (ppb).
McGrane
M
M
National Food Centre, Teagasc, Castleknock, Dublin, Ireland.
O'Keeffe
M
M
Smyth
M R
MR
D000818:Animals; D002845:Chromatography / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008460:Meat / Q000032:analysis*; D010406:Penicillins / Q000032:analysis* / Q000737:chemistry; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis* / Q000737:chemistry
D010406:Penicillins; D019155:Veterinary Drugs