title: Juvenile hormone inhibition of gene expression for cytochrome P4504C1 in adult females of the cockroach, Blaberus discoidalis.
abstract: The regulation and pattern of gene expression for cytochrome P4504C1 was measured in the fat body of adult females of the cockroach, Blaberus discoidalis. The level of CYP4C1-mRNA was high at adult emergence but disappeared after 4 days of adult life. In starved females, CYP4C1-mRNA levels declined by day 4 but increased steadily thereafter; by 25 days, the levels were nearly twice those observed at eclosion. Both the rapid early disappearance of the transcript and the starvation-related increase failed to occur following decapitation. Allatectomy also prevented the disappearance of CYP4C1-mRNA at day 4, and treatment of decapitated females with methoprene (JHA) stimulated a 70% decrease in transcript within 24 h. Injection of synthetic Blaberus hypertrehalosemic hormone (HTH) increased CYP4C1-mRNA by six-fold in the fat body of both intact and decapitated females. CYP4C1-mRNA in the fat body of males did not respond to JHA treatment. The dynamics of CYP4C1-mRNA in the fat body of females could be explained based on an inhibition of CYP4C1 expression by JH that was overcome by HTH.
authors.affiliation: Department of Entomology, Texas A&M University, Texas Agricultural Experiment Station, College Station 77843, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Lu
authors.forename: J Y
authors.initials: JY
authors.lastname: Bradfield
authors.forename: L L
authors.initials: LL
authors.lastname: Keeley
mesh_terms: D000367:Age Factors; D000818:Animals; D003058:Cockroaches / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005247:Feeding Behavior; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007301:Insect Hormones; D007605:Juvenile Hormones / Q000502:physiology*; D008297:Male; D008726:Methoprene; D009479:Neuropeptides; D012333:RNA, Messenger; D012726:Sexual Behavior, Animal
chemical_list: D007301:Insect Hormones; D007605:Juvenile Hormones; D009479:Neuropeptides; D012333:RNA, Messenger; C084112:hypertrehalosemic hormone; D008726:Methoprene; D003577:Cytochrome P-450 Enzyme System