title: Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer).
abstract: The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5.0 (SE = 0.2) and the mean heterozygosity per marker was 0.61 (SE = 0.03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.
authors.affiliation: Wageningen Agricultural University, Department of Environmental Sciences, Wageningen, The Netherlands.
authors.forename: W F
authors.initials: WF
authors.lastname: van Hooft
authors.forename: O
authors.initials: O
authors.lastname: Hanotte
authors.forename: P W
authors.initials: PW
authors.lastname: Wenink
authors.forename: A F
authors.initials: AF
authors.lastname: Groen
authors.forename: Y
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: H H
authors.initials: HH
authors.lastname: Prins
authors.forename: A
authors.initials: A
authors.lastname: Teale
mesh_terms: D000483:Alleles; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005260:Female; D005828:Genetics, Population; D006579:Heterozygote; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity