title: A simple visual immunoassay (VIA) for the semiquantitative determination of plasma elastase levels.
abstract: Elastase levels are an important marker of the extent and severity of inflammatory processes. We describe a visual immunoassay based on two monoclonal antibodies for the semi-quantitative determination of plasma elastase levels. The method is rapid, reliable, easy to perform, and does not require special laboratory equipment. There is coincidence of our results with those obtained by quantitative methods. The assay is suitable to obtain information for the management of patients in most intensive care units.
authors.affiliation: Division of Immunotechnology and Diagnostics, Center for Genetic Engineering and Biotechnology, Havana, Cuba. marta.ayala@cigb.edu.cu
authors.forename: G
authors.initials: G
authors.lastname: Rojas
authors.forename: R M
authors.initials: RM
authors.lastname: Flores
authors.forename: J A
authors.initials: JA
authors.lastname: Galván
authors.forename: A
authors.initials: A
authors.lastname: Vicedo
authors.forename: J V
authors.initials: JV
authors.lastname: Gavilondo
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D019272:Leukocyte Elastase / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D019095:Point-of-Care Systems; D015203:Reproducibility of Results
chemical_list: D019272:Leukocyte Elastase