title: Practical enzymology course based on bioluminescence.
abstract: We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the investigation of kinetic characteristics (kinetic constants) and enzyme-substrate and enzyme-inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico-chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14-17 years.
authors.affiliation: Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russia. krat@bbl.Krasnoyarsk.Su
authors.forename: V A
authors.initials: VA
authors.lastname: Kratasyuk
authors.forename: I Y
authors.initials: IY
authors.lastname: Kudinova
mesh_terms: D001671:Biochemistry / Q000193:education*; D004496:Education, Continuing; D004798:Enzymes; D004800:Enzymes, Immobilized; D006801:Humans; D049449:Luminescence; D017056:Medical Laboratory Personnel / Q000193:education*
chemical_list: D004798:Enzymes; D004800:Enzymes, Immobilized