title: Phosphorylation of tobacco mosaic virus movement protein abolishes its translation repressing ability.
abstract: Previously we showed that the ribonucleoprotein complexes (RNPs) of the TMV 30-kDa movement protein (MP) with TMV RNA are nontranslatable in vitro and noninfectious to protoplasts, but are infectious to intact plants. It has been suggested that MP-TMV RNA complexes could be converted into the translatable and replicatable form in planta in the course of passage through plasmodesmata (Karpova et al., 1997, Virology 230, 11-21). The role of TMV MP phosphorylation was investigated in terms of its capacity to modulate the translation-repressing ability of the MP. Phosphorylation of the TMV MP, either before or after RNP complex formation, caused a conversion of nontranslatable MP-RNA complexes into a form that was translatable in vitro and infectious to protoplasts and plants.
authors.affiliation: Department of Virology, Moscow State University, Moscow, 119899, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Karpova
authors.forename: N P
authors.initials: NP
authors.lastname: Rodionova
authors.forename: K I
authors.initials: KI
authors.lastname: Ivanov
authors.forename: S V
authors.initials: SV
authors.lastname: Kozlovsky
authors.forename: Y L
authors.initials: YL
authors.lastname: Dorokhov
authors.forename: J G
authors.initials: JG
authors.lastname: Atabekov
mesh_terms: D001937:Brassica / Q000201:enzymology / Q000821:virology; D002473:Cell Wall / Q000201:enzymology; D005786:Gene Expression Regulation; D010766:Phosphorylation; D053830:Plant Viral Movement Proteins; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D014026:Nicotiana / Q000201:enzymology / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins; D011494:Protein Kinases; D011493:Protein Kinase C