title: 31P NMR first spectral moment study of the partial magnetic orientation of phospholipid membranes.
abstract: Structural data can be obtained on proteins inserted in magnetically oriented phospholipid membranes such as bicelles, which are most often made of a mixture of long and short chain phosphatidylcholine. Possible shapes for these magnetically oriented membranes have been postulated in the literature, such as discoidal structures with a thickness of one bilayer and with the short acyl chain phosphatidylcholine on the edges. In the present paper, a geometrical study of these oriented structures is done to determine the validity of this model. The method used is based on the determination of the first spectral moment of solid-state (31)P nuclear magnetic resonance spectra. From this first moment, an order parameter is defined that allows a quantitative analysis of partially oriented spectra. The validity of this method is demonstrated in the present study for oriented samples made of DMPC, DMPC:DHPC, DMPC:DHPC:gramicidin A and adriamycin:cardiolipin.
authors.affiliation: Département de Chimie, Centre de Recherche en Sciences et Ingénierie des Macromolécules, Université Laval, Québec, Québec G1K 7P4, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Picard
authors.forename: M J
authors.initials: MJ
authors.lastname: Paquet
authors.forename: J
authors.initials: J
authors.lastname: Levesque
authors.forename: A
authors.initials: A
authors.lastname: Bélanger
authors.forename: M
authors.initials: M
authors.lastname: Auger
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D002308:Cardiolipins / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D006096:Gramicidin / Q000737:chemistry; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D008563:Membrane Lipids / Q000737:chemistry*; D008823:Micelles; D008956:Models, Chemical; D010742:Phospholipid Ethers / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D002308:Cardiolipins; D008081:Liposomes; D008563:Membrane Lipids; D008823:Micelles; D010742:Phospholipid Ethers; D010743:Phospholipids; D006096:Gramicidin; D004317:Doxorubicin; D004134:Dimyristoylphosphatidylcholine