Overproduction of Arabidopsis thaliana FeSOD confers oxidative stress tolerance to transgenic maize.
Transgenic maize (Zea mays L.) plants have been generated by particle gun bombardment that overproduce an Arabidopsis thaliana iron superoxide dismutase (FeSOD). To target this enzyme into chloroplasts, the mature Fesod coding sequence was fused to a chloroplast transit peptide from a pea ribulose-1,5-bisphosphate carboxylase gene. Expression of the chimeric gene was driven by the CaMV 35S promoter. Growth characteristics and in vitro oxidative stress tolerance of transgenic lines grown in control and chilling temperatures were evaluated. The transgenic line with the highest transgenic FeSOD activities had enhanced tolerance toward methyl viologen and had increased growth rates.
Van Breusegem
F
F
Department of Plant Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, Belgium.
Slooten
L
L
Stassart
J M
JM
Moens
T
T
Botterman
J
J
Van Montagu
M
M
Inzé
D
D
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D015870:Gene Expression; D018384:Oxidative Stress; D030821:Plants, Genetically Modified; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics*; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D013482:Superoxide Dismutase