title: Impaired growth on glucose of a pyruvate dehydrogenase-negative mutant of Kluyveromyces lactis is due to a limitation in mitochondrial acetyl-coenzyme A uptake.
abstract: A Kluyveromyces lactis mutant with a disruption in the KlPDA1 gene, encoding the E1 alpha subunit of the pyruvate dehydrogenase complex, exhibited a four-fold reduced specific growth rate on glucose in minimal medium. Growth of the Klpda1 mutant on glucose in complex medium was not affected. Its growth on defined media could be restored by adding amino acids that require mitochondrial acetyl-CoA for their biosynthesis as nitrogen sources. This, together with the observation that low-concentrations of L-carnitine also restored growth on glucose, indicates that the slow-growth phenotype of the Klpda1 mutant is due to a limited capacity of the mitochondria for import of cytosolic acetyl-CoA.
authors.affiliation: Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Zeeman
authors.forename: M A
authors.initials: MA
authors.lastname: Luttik
authors.forename: J T
authors.initials: JT
authors.lastname: Pronk
authors.forename: J P
authors.initials: JP
authors.lastname: van Dijken
authors.forename: H
authors.initials: H
authors.lastname: de Steensma
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D003470:Culture Media; D004251:DNA Transposable Elements; D005816:Genetic Complementation Test; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D007716:Kluyveromyces / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D016296:Mutagenesis; D010641:Phenotype; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003470:Culture Media; D004251:DNA Transposable Elements; D011768:Pyruvate Dehydrogenase Complex; D000105:Acetyl Coenzyme A; D005947:Glucose