title: Cytokine expression and induction of acinar cell apoptosis after pancreatic duct ligation in mice.
abstract: To clarify the role of cytokines and acinar cell apoptosis in the pathogenesis of acute pancreatitis, we investigated the expression of intrapancreatic cytokines and apoptosis-related molecules in mice after pancreatic duct ligation (PDL). From day 1 or 3 after PDL, the expression of interleukin-1alpha (IL-1alpha), IL-1beta, IL-1 receptor antagonist, IL-6, IL-10, and tumor necrosis factor (TNF-alpha) mRNA were up-regulated in the pancreas, suggesting that these cytokines may be involved in the development of pancreatitis after PDL. Acinar cell apoptosis was observed in the pancreas at rates of 0.13 +/- 0.03, 1.32 +/- 0.38, and 0.86 +/- 0.23% on days 1, 3, and 7 after PDL, respectively. Significant increases in intrapancreatic mRNA levels of TNF-alpha, Fas ligand (FasL), and IL-1beta-converting enzyme (ICE) were observed from day 3 after PDL with the appearance of acinar cell apoptosis. The serum amylase activity peaked on day 1 after PDL and gradually decreased on days 3 and 7 after PDL. These results suggest that acinar cell apoptosis induced after PDL may modulate the progression of acute pancreatitis by reducing the release of digestive enzymes and may therefore be a host defense mechanism, and that acinar cell apoptosis after PDL may be mediated by the TNF-alpha and/or Fas/FasL and ICE system.
authors.affiliation: Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan. hyasuda@sun.kpu-m.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Kataoka
authors.forename: H
authors.initials: H
authors.lastname: Ichimura
authors.forename: M
authors.initials: M
authors.lastname: Mitsuyoshi
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: M
authors.initials: M
authors.lastname: Kita
authors.forename: J
authors.initials: J
authors.lastname: Imanishi
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D003250:Constriction; D016207:Cytokines / Q000097:blood / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology; D010183:Pancreatic Ducts / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D000681:Amylases