title: Securinine induced apoptosis in human leukemia HL-60 cells.
abstract: OBJECTIVE
  To study whether securinine might induce apoptosis in human leukemia HL-60 cells.
  
  
  METHODS
  Inhibition of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. DNA fragmentation was visualized by DNA agarose gel electrophoresis and the cellular changes were observed by electron microscope.
  
  
  RESULTS
  Securinine 5-80 mg.L-1 elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in HL-60 cells. Securinine inhibited HL-60 cell proliferation in a time- and concentration-dependent manner. The IC50 and 95% confidence limits were 27 (15-47) mg.L-1 after 12-h treatment with securinine.
  
  
  CONCLUSIONS
  Securinine induced apoptosis in HL-60 cells.
authors.affiliation: Department of Pharmacology, Suzhou Medical College, China.
authors.forename: N Z
authors.initials: NZ
authors.lastname: Dong
authors.forename: Z L
authors.initials: ZL
authors.lastname: Gu
authors.forename: W H
authors.initials: WH
authors.lastname: Chou
authors.forename: C Y
authors.initials: CY
authors.lastname: Kwok
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001381:Azepines; D002455:Cell Division / Q000187:drug effects; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells / Q000648:ultrastructure; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D006801:Humans; D007783:Lactones; D010880:Piperidines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D001381:Azepines; D004273:DNA, Neoplasm; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D007783:Lactones; D010880:Piperidines; C000785:securinine