title: Effect of ethanol on the urinary excretion of cyclohexanol and cyclohexanediols, biomarkers of the exposure to cyclohexanone, cyclohexane and cyclohexanol in humans.
abstract: OBJECTIVE
  This study explored the acute effect of ethanol (EtOH) on the urinary excretion of cyclohexanol (CH-ol), 1,2- and 1,4-cyclohexanediol (CH-diol), biomarkers of exposure to important solvents, and chemical intermediates cyclohexanone (CH-one), cyclohexane (CH) and cyclohexanol.
  
  
  METHODS
  Volunteers (5-8 in each group) were exposed for 8 hours either to CH-one, CH or CH-ol vapor at concentrations of about 200, 1000, and 200 mg/m3, respectively, with concomitant ingestion of EtOH (4 14-g doses taken during the exposure). Urine was collected for 72 hours and analyzed for CH-ol and CH-diols using a procedure involving acidic hydrolysis and gas chromatographic determination.
  
  
  RESULTS
  The metabolic yields of CH-ol, 1,2-, and 1,4-CH-diol, respectively, in the exposures with EtOH were as follows: 11.3%, 36%, 23% after the exposure to CH-one, 3.1%, 15%, 8% after the exposure to CH, and 6.6%, 24%, 18% after the exposure to CH-ol. [The corresponding values obtained previously in matching experiments without EtOH were as follows: 1.0%, 39%, 18% (CH-one); 0.5%, 23%, 11% (CH); and 1.1%, 19%, 8% (CH-ol).] The excretion curves of the metabolites in the exposures with EtOH were not delayed when compared with the corresponding curves of a comparison group.
  
  
  CONCLUSIONS
  The urinary excretion of CH-diols is much less sensitive to EtOH than that of CH-ol. It is recommended to employ CH-diols as useful and more reliable biomarkers of exposure to CH-one, CH and CH-ol in field examinations.
authors.affiliation: National Institute of Public Health, Centre of Industrial Hygiene and Occupational Diseases, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Mráz
authors.forename: E
authors.initials: E
authors.lastname: Gálová
authors.forename: H
authors.initials: H
authors.lastname: Nohová
authors.forename: D
authors.initials: D
authors.lastname: Vítková
authors.forename: M
authors.initials: M
authors.lastname: Tichý
mesh_terms: D000328:Adult; D000434:Alcoholic Beverages; D015415:Biomarkers; D003511:Cyclohexanols / Q000652:urine*; D000431:Ethanol / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D003511:Cyclohexanols; D000431:Ethanol; C064619:1,2-cyclohexanediol