Radiolabelled pyrimidine nucleosides to monitor the expression of HSV-1 thymidine kinase in gene therapy.
Selective radiolabelling and imaging of transduced HSV tk expressing cells was studied using [123I]IVFRU, [125I]FIRU and [125I]FIAU. Although all three radionucleosides accumulated in the KBALB-STK transduced murine tumour line in vitro and in vivo, [125I]FIRU provided optimal performance in terms of selectivity for HSV tk expressing cells and % of injected dose accumulating in the tumor.
Wiebe
L I
LI
Noujaim Institute for Pharmaceutical Oncology Research, U. Alberta, Edmonton, Canada.
Knaus
E E
EE
Morin
K W
KW
D000818:Animals; D015316:Genetic Therapy; D018259:Herpesvirus 1, Human / Q000201:enzymology*; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000628:therapy*; D011741:Pyrimidine Nucleosides / Q000378:metabolism*; D013937:Thymidine Kinase / Q000235:genetics*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D007457:Iodine Radioisotopes; D011741:Pyrimidine Nucleosides; D013937:Thymidine Kinase