Determination of naphthalene metabolites in human urine by liquid chromatography-mass spectrometry with electrospray ionization.
The use of a liquid chromatography-electrospray mass spectrometry system was investigated for the quantitative analysis of naphthalene metabolites (alpha-naphthol, alpha-naphthylglucuronide and beta-naphthylsulphate) in untreated urine samples. Chromatography was carried out under ion-suppressed reversed-phase conditions, by using high-speed (3 cm, 3 microns) columns and formic acid (2 mM) as a modifier in the mobile phase. The ionization was obtained in the negative-ion mode. Linearity, sensitivity and precision of the method were explored by operating in selected-ion monitoring mode. The method was applied to the quantitative analysis of naphthalene metabolites in untreated urine samples from workers in a naphthalene producing plant. Solid-phase extraction was used for sample clean-up and trace enrichment. Liquid chromatography-tandem mass spectrometry experiments were performed for confirmation purposes.
Andreoli
R
R
Dipartimento di Clinica Medica, Nefrologia e Scienze della Prevenzione, Università di Parma, Italy.
Manini
P
P
Bergamaschi
E
E
Mutti
A
A
Franchini
I
I
Niessen
W M
WM
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009281:Naphthalenes / Q000652:urine*; D016273:Occupational Exposure; D015203:Reproducibility of Results
D009281:Naphthalenes; C031721:naphthalene