Determination of the molecular size distribution of Haemophilus influenzae type b-tetanus toxoid conjugate vaccines by size-exclusion chromatography.
The molecular size of Haemophilus influenzae (Hib) type b polysaccharide-protein conjugate vaccines is an important physico-chemical parameter which correlates with immunogenicity. This paper describes the experimental conditions for high-performance size-exclusion chromatography on a PL Aquagel-OH 60 column to determine the size distribution of Hib high-molecular-weight conjugate vaccines.
Parisi
L
L
Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Rome, Italy.
von Hunolstein
C
C
D001428:Bacterial Vaccines / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D006193:Haemophilus influenzae / Q000276:immunology*; D008970:Molecular Weight; D013745:Tetanus Toxoid / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry*
D001428:Bacterial Vaccines; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic