title: Cytokines increase endothelin ETB receptor contractile activity in rat cerebral artery.
abstract: The plasticity of endothelin ETB receptor activity and the influence of pro-inflammatory cytokines was examined in cerebral artery. In fresh rat basilar artery, the selective ETB receptor agonist, sarafotoxin S6c, induced negligible contractions. However, after 1 day of organ culture, fully defined concentration-response curves were obtained in artery segments exposed to sarafotoxin S6c. Organ culture in the presence of either interleukin-1 beta or tumour necrosis factor-alpha, but not interleukin-2 or interleukin-6, further amplified the maximal contraction to sarafotoxin S6c. The plasticity of ETB receptor expression in cerebral arteries and sensitivity for pro-inflammatory cytokines, suggest a role in inflammatory cerebral diseases such as stroke.
authors.affiliation: Department of Neurology, University Hospital of Trondheim, Norway.
authors.forename: K H
authors.initials: KH
authors.lastname: Leseth
authors.forename: M
authors.initials: M
authors.lastname: Adner
authors.forename: H K
authors.initials: HK
authors.lastname: Berg
authors.forename: L R
authors.initials: LR
authors.lastname: White
authors.forename: J
authors.initials: J
authors.lastname: Aasly
authors.forename: L
authors.initials: L
authors.lastname: Edvinsson
mesh_terms: D000818:Animals; D002536:Cerebral Arteries / Q000187:drug effects* / Q000502:physiology*; D016207:Cytokines / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D009924:Organ Culture Techniques; D051381:Rats; D011921:Rats, Inbred WKY; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000187:drug effects / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects* / Q000502:physiology*; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014757:Viper Venoms / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D014409:Tumor Necrosis Factor-alpha; D014662:Vasoconstrictor Agents; D014757:Viper Venoms; C057354:sarafotoxins s6