[The modulation of cell proliferation induced by growth factors in human retinal pigment epithelial cells].
OBJECTIVE
To explore the modulation of cell proliferation induced by growth factors in cultured human retinal pigment epithelial (RPE) cells.


METHODS
A well-cultured system of human fetal RPE cells was established to determine the effects of several growth factors, such as epidermal growth factor (EGF), basic-fibroblast growth factor (b-FGF), insulin-like growth factor (IGF-I), on the cultured human RPE cells by using 3H-thymidine incorporation and cell count.


RESULTS
(1) EGF, b-FGF, IGF-I significantly increased the DNA 6.6-12.2 times in human RPE cells in comparison with the control, the rank order of potency of the factors being EGF > b-FGF > IGF-I. (2) When the factors were used in combination, the above factors caused a significant enhancement of 3H-Tdr incorporation by times.


CONCLUSIONS
It is suggested that the synergetic effects of growth factors and their complex interactions play a role in the development of proliferative retinopathy.
Cao
J
J
Department of Ophthalmology, Third Hospital, Beijing Medical University.
Zhang
H
H
Liu
N
N
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology*; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology*
D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I