title: Nonrandom degradation of DNA in human leukemic cells during radiation-induced apoptosis.
abstract: In many cells, the process of apoptosis is accompanied by endonuclease-mediated double-strand cleavage of DNA between nucleosomes, resulting in the production of discrete fragments of 200 bp or multiples thereof. To address the question of whether this endonuclease attack occurs randomly or nonrandomly along chromosomes, we first constructed chromosome fluorescence in situ hybridization probes from the 200- and 400-bp fragments from gamma-irradiated apoptotic human T cells along with similar-sized probes from randomly sheared DNA of nonirradiated cells. These probes were compared for their binding along normal human metaphase chromosomes after fluorescence in situ hybridization with and without the presence of unlabeled total human blocking DNA. The addition of blocking DNA to the apoptotic probes revealed a nonrandom pattern of hybridization that was not observed for the nonirradiated control probes. The most obvious areas of selective binding occurred around the centromeric and other heterochromatic regions along the chromosome arms, such as the long (q arm) of the Y chromosome. The converse of this experiment was also carried out. DNA probes from heterochromatic and euchromatic regions of the human Y chromosome were hybridized onto slot blots of apoptotic ladder-sized and randomly sheared nonirradiated human T-lymphocyte DNA. The slot blot results showed that for an equal mass of ladder-sized apoptotic DNA and randomly sheared nonirradiated control DNA, the apoptotic DNA sample contains a relatively larger proportion of Y heterochromatin DNA sequences (approximately 2.5-fold). Together, these results indicate that apoptosis-mediated endonuclease attack does not occur randomly in the genome but occurs preferentially in heterochromatin.
authors.affiliation: Department of Radiological Health Sciences, Colorado State University, Fort Collins 80523-1673, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Dullea
authors.forename: J F
authors.initials: JF
authors.lastname: Robinson
authors.forename: J S
authors.initials: JS
authors.lastname: Bedford
mesh_terms: D000208:Acute Disease; D000328:Adult; D017209:Apoptosis / Q000528:radiation effects*; D002503:Centromere / Q000528:radiation effects; D002877:Chromosomes, Human / Q000528:radiation effects* / Q000648:ultrastructure; D004249:DNA Damage; D053938:DNA Fragmentation / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004720:Endonucleases / Q000378:metabolism; D006367:HeLa Cells / Q000528:radiation effects; D006570:Heterochromatin / Q000528:radiation effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019169:Jurkat Cells / Q000473:pathology / Q000528:radiation effects; D007938:Leukemia / Q000473:pathology* / Q000532:radiotherapy; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000528:radiation effects; D014998:Y Chromosome / Q000528:radiation effects
chemical_list: D004273:DNA, Neoplasm; D006570:Heterochromatin; D009363:Neoplasm Proteins; D004720:Endonucleases