title: Effect of environmental haemin upon the physiology and biochemistry of Prevotella intermedia R78.
abstract: The effect of environmental haemin on the physiology and biochemistry of Prevotella intermedia R78 grown in batch culture was assessed. Extent and rate of growth increased as the environmental haemin concentration was raised. In addition, cell morphology was predominantly cocco-bacillary when cultured in high haemin environments, while bacillary forms were prevalent in low haemin conditions (< 2.5 mumol l-1). Cells harvested from low haemin environments produced greater numbers of extracellular vesicles and greater amounts of peptidolytic activity, haemagglutinating potential and haemin binding activity when compared with cells harvested from high haemin conditions. The results of the present study indicate that aspects of the biochemistry and physiology of P. intermedia are influenced by changes in environmental haemin levels.
authors.affiliation: Anaerobe Reference Unit, Public Health Laboratory, University Hospital of Wales, Cardiff, UK.
authors.forename: S
authors.initials: S
authors.lastname: Stubbs
authors.forename: J A
authors.initials: JA
authors.lastname: Hobot
authors.forename: R J
authors.initials: RJ
authors.lastname: Waddington
authors.forename: G
authors.initials: G
authors.lastname: Embery
authors.forename: M A
authors.initials: MA
authors.lastname: Lewis
mesh_terms: D003470:Culture Media; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D006384:Hemagglutination; D006427:Hemin / Q000378:metabolism*; D006868:Hydrolysis; D018713:Prevotella intermedia / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D014617:Vacuoles / Q000502:physiology
chemical_list: D003470:Culture Media; D006427:Hemin; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C059607:glycylprolyl dipeptidase