Effect of passive immunotherapy on murine gut-derived sepsis caused by Pseudomonas aeruginosa.
The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampicillin (200 mg/kg) to disrupt the normal bacterial flora. Cyclophosphamide was then administered to induce translocation of P. aeruginosa that had colonised the gastrointestinal tract so that gut-derived septicaemia was produced. In this model, intraperitoneal administration of antiserum against heat-killed bacteria, 100 microl/mouse, twice a day for 3 consecutive days significantly increased the survival rate over that of mice treated with normal rabbit serum. Antiserum against elastase, alkaline protease, or a combination of these two antisera, failed to provide significant protection. In contrast, antiserum against exotoxin A significantly increased the survival rate over that of mice treated with normal rabbit serum. These results indicate that passive immunisation with antiserum against heat-killed bacteria and exotoxin A, but not with antiserum against either elastase or alkaline protease, protects mice against gut-derived sepsis caused by P. aeruginosa.
Matsumoto
Tetsuya
T
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
Furuya
Nobuhiko
N
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
Tateda
Kazuhiro
K
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
Miyazaki
Shuichi
S
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
Ohno
Akira
A
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
Ishii
Yoshikazu
Y
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
Hirakata
Yoichi
Y
Department of Laboratory Medicine, Nagasaki University School of Medicine, Sakamoto, Nagasaki, Japan.
Yamaguchi
Keizo
K
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540.
D036002:ADP Ribose Transferases; D000818:Animals; D001427:Bacterial Toxins; D005098:Exotoxins / Q000276:immunology; D006358:Hot Temperature; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology; D007116:Immunization, Passive; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051379:Mice; D010196:Pancreatic Elastase / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D018805:Sepsis / Q000517:prevention & control*; D012697:Serine Endopeptidases / Q000276:immunology; D016019:Survival Analysis; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D007106:Immune Sera; D010196:Pancreatic Elastase; D012697:Serine Endopeptidases; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C032923:microbial serine proteinases