Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP.
The repetitive sequence of Leishmania major gene B protein (GBP) has previously been shown to be a useful tool in the diagnosis of cutaneous leishmaniasis (CL). Here, we have assessed enzyme-linked immunosorbent assays (ELISAs) using recombinant L. donovani GBP (rGBP) and a peptide sequence of L. donovani GBP (GBPP) in the diagnosis of L. donovani infections in Sudan. The sensitivity of the rGBP ELISA in diagnosing visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) was 92% and 93%, respectively. In contrast, the sensitivity of the GBPP ELISA was 55% for VL and 63% for PKDL. Plasma antibody reactivity of donors with VL and PKDL remained high for an extended period after the end of treatment. Antibody-reactivity to rGBP and GBPP was detected in 71% and 14% of plasma samples from CL patients, respectively. Plasma from healthy Sudanese donors living in an area endemic for malaria but free of leishmaniasis was negative in both assays.
Jensen
A T
AT
Centre for Medical Parasitology at Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark. parasite@biobase.dk
Gasim
S
S
Moller
T
T
Ismail
A
A
Gaafar
A
A
Kemp
M
M
el Hassan
A M
AM
Kharazmi
A
A
Alce
T M
TM
Smith
D F
DF
Theander
T G
TG
D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007893:Leishmania donovani / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015800:Protozoan Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D020449:Repetitive Sequences, Amino Acid; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins