title: Differences in osteopontin up-regulation between proximal and distal tubules after renal ischemia/reperfusion.
abstract: BACKGROUND
  Osteopontin (OPN) is a highly acidic phosphoprotein containing an arginine-glycine-aspartic acid (RGD) cell adhesion motif. High OPN expression has been found in tissues with high cell turnover, and OPN up-regulation has been demonstrated in several models of renal injury, suggesting a possible role in tissue remodeling and repair. However, its exact function in the kidney remains unknown. In this study, the possible contribution of OPN to regeneration and repair in the kidney was explored by studying the time course and subcellular localization of OPN up-regulation after renal ischemia/reperfusion injury in different nephron segments and by investigating its relationship with tubular morphology.
  
  
  METHODS
  Rats that underwent 60 minutes of left renal ischemia and a right nephrectomy sacrificed at 10 different time points (from 1 hr to 10 days after reperfusion) were compared with uninephrectomized rats at each time point. In renal tissue sections immunostained for OPN, proximal (PTs) and distal tubules (DTs) in both the renal cortex and outer stripe of the outer medulla (OSOM) were scored for the degree of OPN expression and tubular morphology.
  
  
  RESULTS
  Kidneys of uninephrectomized rats showed no injury, and the localization and intensity of their OPN expression remained unaltered compared with normal rats. After ischemia/reperfusion, morphological damage was most severe in PTs of the OSOM, but all examined nephron segments showed a significant increase in OPN expression. The time course of OPN up-regulation was different in PTs and DTs. DTs in both cortex and OSOM rapidly increased their OPN expression, with a maximum at 24 hours after reperfusion followed by a slow decrease. In contrast, PTs showed a delayed increase in OPN staining, with a maximum after five to seven days, higher in the OSOM than in the cortex. In OSOM PTs, OPN expression was predominantly associated with morphological regeneration, whereas DTs showed a substantial OPN up-regulation without major morphological damage. PTs and DTs displayed a different subcellular OPN staining pattern: OPN staining in DTs was located to the apical side of the cell; PTs, however, presented a vesicular, perinuclear staining pattern.
  
  
  CONCLUSIONS
  Our study found a different pattern of OPN up-regulation after renal ischemia/reperfusion in PTs versus DTs, both with regard to time course and subcellular localization. DTs show an early and persistent increase in OPN staining in the absence of major morphological injury, whereas OPN staining in PTs is delayed and is mostly associated with morphological regeneration. PTs show a vesicular, perinuclear OPN staining pattern, whereas DTs show OPN staining at the apical cell side.
authors.affiliation: Department of Nephrology, University of Antwerp, Belgium.
authors.forename: V P
authors.initials: VP
authors.lastname: Persy
authors.forename: W A
authors.initials: WA
authors.lastname: Verstrepen
authors.forename: D K
authors.initials: DK
authors.lastname: Ysebaert
authors.forename: K E
authors.initials: KE
authors.lastname: De Greef
authors.forename: M E
authors.initials: ME
authors.lastname: De Broe
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D000818:Animals; D003404:Creatinine / Q000097:blood; D007686:Kidney Tubules, Distal / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009336:Necrosis; D053495:Osteopontin; D051381:Rats; D011917:Rats, Inbred Lew; D012038:Regeneration; D012079:Renal Circulation / Q000502:physiology; D015427:Reperfusion Injury / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D012795:Sialoglycoproteins / Q000032:analysis / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D003404:Creatinine