DNA immunization is associated with increased activity of type I iodothyronine 5'-deiodinase in mouse liver.
Inflammatory cytokines in vitro are believed to be involved in the regulation of type I iodothyronine 5'-deiodinase (5'-DI) activity. The present study was undertaken to investigate in vivo effects of DNA immunization of mice on the 5'-DI activity in the liver. A mammalian expression vector encoding the beta-galactosidase (pCMV-betagal) was used for intradermal immunization. Furthermore, immunostimulatory CpG motifs, which induce the expression of IL-6, IL-12, IL-18, TNF-alpha/beta and IFN-gamma were coinjected as oligodeoxynucleotides. From our data we conclude that the activity of 5'-DI in mouse liver when compared to non-immunized animals (100%) was found to be significantly enhanced by DNA immunization 2 weeks (175.7%) or 3 weeks (192.6%) after the plasmid injection. In addition, the activity of the 5'-DI in mouse liver was markedly enhanced 2 weeks (252.4%) or 3 weeks (243.3%) after the injection when CpG motifs were applied together with the plasmid DNA.
Brtko
J
J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Mostböck
S
S
Scheiblhofer
S
S
Hartl
A
A
Thalhamer
J
J
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004247:DNA / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D007114:Immunization; D007249:Inflammation / Q000276:immunology; D007453:Iodide Peroxidase / Q000096:biosynthesis / Q000276:immunology*; D008099:Liver / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D016207:Cytokines; D004247:DNA; D007453:Iodide Peroxidase