Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed-bed reactors.
The supply of oxygen is a crucial parameter when cultivating animal cells in fixed-bed reactors because of the reaction-diffusion limitation within the porous carriers. To reduce limitation and increase productivity, the dissolved oxygen concentration was raised to above air saturation (hyperoxia) in long-term experiments using hybridoma cultures. This resulted in a threefold increase of the steady-state antibody production at high dilution rates compared to air saturated medium. A reaction-diffusion model was developed as a tool to describe the oxygen distribution in fixed-bed systems. The model corresponded well to the experimental data. It was also used to study the influence of several parameters on the performance of the fixed-bed system, such as the carrier size, the dissolved oxygen concentration, or the superficial flow velocity. By adapting the model it was shown that reaction-diffusion limitation is generally not a problem for other substrates such as glucose or glutamine.
Fassnacht
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Technische Universität Hamburg-Harburg, Germany.
Pörtner
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D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006825:Hybridomas / Q000378:metabolism*; D000860:Hypoxia; D007700:Kinetics; D051379:Mice; D008962:Models, Theoretical; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D005973:Glutamine; D005947:Glucose; D010100:Oxygen