Carrier-mediated transport systems for glucose in mucosal cells of the human oral cavity.
The in vitro uptake study was performed using the isolated cells of human oral mucosa, buccal and the dorsum of the tongue, to investigate the mechanisms of glucose uptake. The uptake of D-glucose was much larger in cells of the dorsum of the tongue than in buccal cells and was inhibited more extensively by 2-deoxy-D-glucose, a substrate of facilitative glucose transporters, than by alpha-methyl-D-glucoside, a specific substrate of SGLT1, suggesting the larger contribution of a facilitative transporter than Na(+)/glucose cotransporter. Furthermore, from the results of inhibition studies by several sugar analogues including maltose and D-mannose, GLUT1 and/or GLUT3 were suggested to take part in the glucose uptake by oral mucosa. Therefore, we have attempted to confirm the expression of glucose transporters on the oral mucosa by employing Western blotting. As a result, it was suggested that SGLT1, GLUT1, GLUT2, and GLUT3 are expressed in the epithelial cells of human oral mucosa.
Oyama
Y
Y
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Yamano
H
H
Ohkuma
A
A
Ogawara
K
K
Higaki
K
K
Kimura
T
T
D000042:Absorption; D000328:Adult; D000963:Antimetabolites / Q000378:metabolism / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D015153:Blotting, Western; D003847:Deoxyglucose / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000493:pharmacokinetics*; D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; D051274:Glucose Transporter Type 3; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D051269:Sodium-Glucose Transporter 1; D014059:Tongue / Q000166:cytology / Q000378:metabolism
D000963:Antimetabolites; D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; D051274:Glucose Transporter Type 3; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; C495674:SLC5A1 protein, human; D051269:Sodium-Glucose Transporter 1; D003847:Deoxyglucose; D005947:Glucose