Simultaneous detection of multiple STR loci on sex chromosomes for forensic testing of sex and identity.
The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS390/ DYS393), and 2 duplex-PCRs, each using an X- and a Y-STR (ARA/DYS390 and ARA/DYS393), and detection of PCR products by using an automated DNA sequencer are reported herein. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or Y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of population data, and sex determination of a biological sample from a single PCR.
Tun
Z
Z
Department of Legal Medicine, Osaka University Graduate School of Medical, Japan.
Honda
K
K
Nakatome
M
M
Nakamura
M
M
Shimada
S
S
Ogura
Y
Y
Kuroki
H
H
Yamazaki
M
M
Terada
M
M
Matoba
R
R
D000483:Alleles; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012730:Sex Chromosomes / Q000235:genetics*; D012732:Sex Determination Analysis / Q000379:methods*
D017931:DNA Primers; D004247:DNA