[Calcium mobilization and influx induced by extracellular ATP in the lens epithelial cells].
OBJECTIVE
To examine the effects of extracellular ATP application on the intracellular Ca++ concentration of the in vitro lens epithelial cells (LECs).


METHODS
LECs were loaded with fluo-3, then the changes of fluorescent intensities in different conditions were observed by laser scanning confocal microscopy (LSCM).


RESULTS
Normal in vitro LECs have strong response to 100 mumol/L ATP and a biphasic wave rise is shown, but LECs have no apparent response to low concentration ATP. When the extracellular calcium chelates to ethylene glycol bis (2-aminoethyl) ether tetracetic acid (EGTA), ATP stimulates the cells and the response is a rapid monophasic rise.


CONCLUSIONS
ATP can induce calcium mobilization and entry into LECs loaded with fluo-3 in a dose-dependent manner. In the LEC membrane, possibly there are P2-purinoceptors.
Wang
F
F
Department of Ophthalmology, General Hospital of PLA, Beijing.
He
S
S
Li
N
N
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002614:Chelating Agents; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D002118:Calcium