title: Genetic and biochemical evidence for the involvement of alpha-1,4 glucanotransferases in amylopectin synthesis
abstract: We describe a novel mutation in the Chlamydomonas reinhardtii STA11 gene, which results in significantly reduced granular starch deposition and major modifications in amylopectin structure and granule shape. This defect simultaneously leads to the accumulation of linear malto-oligosaccharides. The sta11-1 mutation causes the absence of an alpha-1,4 glucanotransferase known as disproportionating enzyme (D-enzyme). D-enzyme activity was found to be correlated with the amount of wild-type allele doses in gene dosage experiments. All other enzymes involved in starch biosynthesis, including ADP-glucose pyrophosphorylase, debranching enzymes, soluble and granule-bound starch synthases, branching enzymes, phosphorylases, alpha-glucosidases (maltases), and amylases, were unaffected by the mutation. These data indicate that the D-enzyme is required for normal starch granule biogenesis in the monocellular alga C. reinhardtii.
authors.affiliation: Laboratoire de Chimie Biologique, Unite Mixte de Recherche du Centre National de la Recherche Scientifique no. 8576, Universite des Sciences et Technologies de Lille, 59655 Villeneuve D'Ascq cedex France (C.C., D.D., G.M., B.D., C.d.H., S.B.).
authors.initials: C
authors.lastname: Colleoni
authors.lastname: Dauvill e D
authors.initials: G
authors.lastname: Mouille
authors.lastname: Bul on A
authors.initials: D
authors.lastname: Gallant
authors.initials: B
authors.lastname: Bouchet
authors.initials: M
authors.lastname: Morell
authors.initials: M
authors.lastname: Samuel
authors.initials: B
authors.lastname: Delrue
authors.initials: C
authors.lastname: d'Hulst
authors.initials: C
authors.lastname: Bliard
authors.initials: JM
authors.lastname: Nuzillard
authors.initials: S
authors.lastname: Ball