[Differentiation of lens epithelial cell and effects of serum and ocular tissue on its proliferation in vitro].
OBJECTIVE
To study the pathogenesis of lens posterior capsular opacification (PCO) and the effects of the breakdown of blood-aqueous barrier and remain of lens cortex on PCO in cell culture level.


METHODS
The differentiation of cultured bovine lens epithelial cells (BLEC) was observed by Coomassie BB staining, light and electron microscope. The effects of fetal bovine serum, aqueous humor and lens cortex, etc., on the proliferation of BLEC were tested by Giemsa-staining colorimetry.


RESULTS
BLEC in vitro could gradually differentiate into lens fiber cells or lens fibers in 1-7 passages. The characteristics of differentiation were that the shape of cells changed from polygon into fusiform and fibriform, the bulk enlarged, and cytoskeleton gradually increased. Fetal bovine serum promoted the proliferation of BLEC depending on its concentration (P < 0.05). High concentration of aqueous humor inhibited the proliferation (P < 0.01). Supernatant and suspension of lens cortex, supernatant of lens nucleus, vitreous humor could promote the proliferation (P < 0.01).


CONCLUSIONS
The differentiation of lens epithelial cells plays important roles in PCO, the breakdown of blood-aqueous barrier, the remain of lens cortex and the prolapse of vitreous may promote PCO by stimulating the proliferation of lens epithelial cells.
Li
Q
Q
Department of Ophthalmology, Xinhuan Hospital, Shanghai Second Medical University.
Lu
D
D
Wang
L
L
D000818:Animals; D001082:Aqueous Humor / Q000502:physiology; D001790:Blood Physiological Phenomena; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure*; D007908:Lens, Crystalline / Q000166:cytology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
D003470:Culture Media