Analysis and confirmation of synthetic anorexics in adulterated traditional Chinese medicines by high-performance capillary electrophoresis.
Six synthetic anorexics, clobenzorex, diethylpropion, fenfluramine, methamphetamine, phenylpropanolamine and phentermine, which can be found as adulterants in traditional Chinese medicines were assayed simultaneously by high-performance capillary electrophoresis. The electrolyte was a buffer solution containing 120 mM phosphate buffer (NaH2PO4/H3PO4, pH 2.0) and 15% acetonitrile. Applied voltage was 16 kV and temperature was 30 degrees C. Fluoren-2,7-diammonium chloride was used as an internal standard and detector set at 200 nm. The recoveries of the synthetic anorexic adulterants in traditional Chinese medicinal formula using C8-SCX mixed solid-phase extraction were studied. Several traditional Chinese medicinal powders obtained from clinics were also studied by the above HPCE method and confirmed by GC-MS. Clobenzorex, diethylpropion and fenfluramine were found and determine in these samples.
Ku
Y R
YR
Institute of Chinese Pharmaceutical Sciences, China Medical College, Taichung, Taiwan.
Chang
Y S
YS
Wen
K C
KC
Ho
L K
LK
D001067:Appetite Depressants / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D008516:Medicine, Chinese Traditional; D015203:Reproducibility of Results
D001067:Appetite Depressants; D004365:Drugs, Chinese Herbal