Analysis of transthyretin amyloid fibrils from vitreous samples in familial amyloidotic polyneuropathy (Val30Met).
The aim of the present study was to analyze the forms of wild type and mutated monomeric transthyretin (Val30Met) in the amyloid fibrils of patients with familial amyloidotic polyneuropathy by electrospray ionization mass spectrometry (ESI-MS). The solubility of amyloid fibrils from the vitrectomized samples was examined to determine the appropriate solution for ESI-MS. ESI-MS analysis revealed that heterozygotic Val30Met amyloid fibrils contained 14.6 +/- 7.5% normal TTR. In all samples, 3 different types of variant ATTR could be identified: Full length ATTR, and -57, and -157 (or 156) Da from ATTR Val30Met were found. The two peaks showing -57, and -157 (or 156) Da from ATTR Val30Met corresponded to the -Gly, and -Gly-Pro sequences of ATTR Val30Met from the N-terminal. The results illustrate the heterogeneity of ATTR amyloid deposits and this method may be very useful for analyzing amyloid fibrils in ATTR related amyloidosis.
Ando
Y
Y
Department of Internal Medicine, Umeå University, Sweden.
Ando
E
E
Ohlsson
P I
PI
Olofsson
A
A
Sandgren
O
O
Suhr
O
O
Terazaki
H
H
Obayashi
K
K
Lundgren
E
E
Ando
M
M
Negi
A
A
D000368:Aged; D019943:Amino Acid Substitution; D017772:Amyloid Neuropathies / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008715:Methionine / Q000737:chemistry / Q000235:genetics; D008875:Middle Aged; D008970:Molecular Weight; D011228:Prealbumin / Q000737:chemistry* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014633:Valine / Q000737:chemistry / Q000235:genetics; D014822:Vitreous Body / Q000737:chemistry
D011228:Prealbumin; D008715:Methionine; D014633:Valine