title: Rat peroxisome proliferator-activated receptors and brown adipose tissue function during cold acclimatization.
abstract: Brown adipose tissue (BAT) hyperplasia is a fundamental physiological response to cold; it involves an acute phase of mitotic cell growth followed by a prolonged differentiation phase. Peroxisome proliferator-activated receptors (PPARs) are key regulators of fatty acid metabolism and adipocyte differentiation and may therefore mediate important metabolic changes during non-shivering thermogenesis. In the present study we have investigated PPAR mRNA expression in relation to peroxisome proliferation in rat BAT during cold acclimatization. By immunoelectron microscopy we show that the number of peroxisomes per cytoplasmic volume and acyl-CoA oxidase immunolabeling density remained constant (thus increasing in parallel with tissue mass and cell number) during the initial proliferative phase and the acute thermogenic response but increased after 14 days of cold exposure, correlating with terminal differentiation of BAT. A pronounced decrease in BAT PPARalpha and PPARgamma mRNA levels was found within hours of exposure to cold, which was reversed after 14 days, suggesting a role for either or both of these subtypes in the proliferation and induction of peroxisomes and peroxisomal beta-oxidation enzymes. In contrast, PPARdelta mRNA levels increased progressively during cold exposure. Transactivation assays in HIB 1B and HEK-293 cells demonstrated an adrenergic stimulation of peroxisome proliferator response element reporter activity via PPAR, establishing a role for these nuclear receptors in hormonal regulation of gene transcription in BAT.
authors.affiliation: Center for Biotechnology, Huddinge University Hospital, Karolinska Institute, S-141 86, Huddinge, Sweden.
authors.forename: H M
authors.initials: HM
authors.lastname: Guardiola-Diaz
authors.forename: S
authors.initials: S
authors.lastname: Rehnmark
authors.forename: N
authors.initials: N
authors.lastname: Usuda
authors.forename: T
authors.initials: T
authors.lastname: Albrektsen
authors.forename: D
authors.initials: D
authors.lastname: Feltkamp
authors.forename: J A
authors.initials: JA
authors.lastname: Gustafsson
authors.forename: S E
authors.initials: SE
authors.lastname: Alexson
mesh_terms: D042962:Acyl-CoA Oxidase; D000222:Adaptation, Physiological / Q000235:genetics; D002001:Adipose Tissue, Brown / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D019748:Carbon-Carbon Double Bond Isomerases / Q000235:genetics; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics; D064006:Dodecenoyl-CoA Isomerase; D005260:Female; D007150:Immunohistochemistry; D008071:Lipoprotein Lipase / Q000235:genetics; D008297:Male; D008830:Microbodies / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D008071:Lipoprotein Lipase; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase