title: Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
abstract: Using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) we studied the decomposition pathway of a prooligonucleotide during incubation in a crude cell extract. This study, involving no sample processing, except drop dailysis to remove salts, led us to identify more than thirty metabolites from several metabolic pathways.
authors.affiliation: Laboratoire de Chimie Bioorganique, U.M.R. C.N.R.S. 5625, Case Courrier 008, Université Montpellier II, Place Eugéne Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: T
authors.initials: T
authors.lastname: Lioux
authors.forename: I
authors.initials: I
authors.lastname: Lefebvre
authors.forename: J J
authors.initials: JJ
authors.lastname: Vasseur
authors.forename: J L
authors.initials: JL
authors.lastname: Imbach
mesh_terms: D000818:Animals; D002460:Cell Line; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D009841:Oligonucleotides