Development and testing of a microbiological assay to detect residual effects of disinfectant on hard surfaces.
We describe a glucuronidase bioassay for detecting residual bactericidal activity from the use of disinfectants on hard surfaces; in this assay we used formaldehyde, ethanol, isopropanol, chlorine, and a commercial preparation containing 2-bromo-2-nitro-1, 3-propanediol. Chlorine and the commercial preparation showed bactericidal activity (53.5% and 98.2%, respectively) for a week after disinfection.
Mariscal
A
A
Department of Preventive Medicine and Public Health, Facultad de Medicina, Universidad de Málaga, E-29071 Málaga, Spain. mariscal@uma.es
Carnero-Varo
M
M
Gómez-Aracena
J
J
Fernández-Crehuet
J
J
D019840:2-Propanol / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D002713:Chlorine / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D000431:Ethanol / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005966:Glucuronidase / Q000032:analysis; D008828:Microbiological Techniques; D011409:Propylene Glycols / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors
D004202:Disinfectants; D011409:Propylene Glycols; D005557:Formaldehyde; D000431:Ethanol; D002713:Chlorine; C006827:bronopol; D005966:Glucuronidase; D019840:2-Propanol