title: Human mannan-binding lectin inhibits the infection of influenza A virus without complement.
abstract: Mannan-binding lectin (MBL) is a C-type serum lectin that is believed to play an important role in innate immunity. It is one of the collectin family, which is characterized by having a collagen-like sequence and a carbohydrate recognition domain. MBL can bind to sugar determinants of several micro-organisms, neutralize them and inhibit infection by complement activation through the lectin pathway and opsonization by collectin receptors. Bovine conglutinin and mouse MBL inhibit the infective and haemagglutinating activities of influenza A viruses. To identify the direct antiviral activity of human MBL against influenza A viruses that does not depend on complement activation or opsonization, we isolated native MBL from human serum and produced a recombinant MBL in Chinese hamster ovary (CHO) cells using a pNOW/CMV-A expression vector system. Native and recombinant human MBL exhibited neutralization activity against A/Ibaraki/1/90 (H3N2), with the plaque focus reduction assay at the viral attachment phase. Their activities were inhibited by EDTA, mannose and anti-human MBL antibody. Furthermore, at the viral expansion phase both MBL in culture medium prevented viral spreading from primary infected cells to neighbour cells. A virus recovery study using EDTA indicated that interaction between MBL and virus was reversible and non-damaging to the virus. Lectin blot and immunohistochemistry assays showed that these antiviral activities involved binding between MBL and two viral envelope proteins, haemagglutinin and neuraminidase. These findings suggest that human MBL can play an important role in innate immunity by direct viral neutralization and inhibition of viral spread, as well as an indirect role through opsonization and complement activation.
authors.affiliation: Departments of Virology, Osaka Prefectural Instititute of Public Health, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kase
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kawai
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: K
authors.initials: K
authors.lastname: Ohtani
authors.forename: S
authors.initials: S
authors.lastname: Eda
authors.forename: A
authors.initials: A
authors.lastname: Maeda
authors.forename: Y
authors.initials: Y
authors.lastname: Okuno
authors.forename: T
authors.initials: T
authors.lastname: Kurimura
authors.forename: N
authors.initials: N
authors.lastname: Wakamiya
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D018929:Cell Culture Techniques; D037222:Collectins; D003165:Complement System Proteins / Q000276:immunology; D006224:Cricetinae; D003412:Cricetulus; D006387:Hemagglutination, Viral; D006389:Hemagglutinins, Viral / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development; D007251:Influenza, Human / Q000276:immunology*; D037102:Lectins / Q000378:metabolism; D008351:Mannans / Q000378:metabolism*; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism; D009500:Neutralization Tests
chemical_list: D002352:Carrier Proteins; D037222:Collectins; D006389:Hemagglutinins, Viral; D037102:Lectins; D008351:Mannans; D003165:Complement System Proteins; D009439:Neuraminidase