title: Serodiagnosis of goat warble fly infestation by Przhevalskiana silenus with a commercial ELISA kit.
abstract: Cross-reactivity between Hypoderma lineatum antigen and anti-Przhevalskiana silenus antibodies has been demonstrated by an ELISA technique. To evaluate the applicability of a commercial ELISA kit for the immunodiagnosis of goat warble fly infestation, different dilutions of serum and conjugate were tested, the development of antibody to P silenus in naturally infested goats was studied, and the results were compared with an ELISA technique using an antigen extracted from the first instar larvae of H lineatum. The best results were obtained with a serum dilution of 1:50; with both techniques the highest antibody concentration were recorded in October, November and December. In view of the confirmed cross-reactivity between H lineatum antigen and anti-P silenus antibodies, and the simplicity and rapidity of the assay, the commercial ELISA kit can be considered as a useful tool for the diagnosis of goat warble fly infestation.
authors.affiliation: Dipartmento di Sanità Benessere Animale, Facoltá di Medicina Veterinaria, Università Degli studi di Bari, Valenzano, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Otranto
authors.forename: C
authors.initials: C
authors.lastname: Boulard
authors.forename: A
authors.initials: A
authors.lastname: Giangaspero
authors.forename: M P
authors.initials: MP
authors.lastname: Caringella
authors.forename: D
authors.initials: D
authors.lastname: Rimmele
authors.forename: V
authors.initials: V
authors.lastname: Puccini
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D004175:Diptera / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D015511:Goat Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D006041:Goats; D007000:Hypodermyiasis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary
chemical_list: D000906:Antibodies