title: Effects of genistein on aggregation and cytosolic free calcium in pig platelets.
abstract: OBJECTIVE
  To study the effects of genistein on aggregation and cytosolic free calcium concentration in platelets.
  
  
  METHODS
  Using turbidimetry to analyse aggregation and using Fura-2 fluorescence technique to determine Ca2+ level.
  
  
  RESULTS
  Genistein strongly inhibited the pig platelet aggregation induced by thrombin (250 U.L-1). When genistein concentrations were 5 and 20 mumol.L-1, the inhibition rates on the aggregation were 52% and 73%, respectively. Genistein inhibited the rise of cytosolic free calcium concentration in platelets stimulated by thrombin (500 U.L-1) in the presence of extracellular Ca2+ 1 mmol.L-1. When genistein concentrations were 10, 20, 40, and 80 mumol.L-1, the inhibition rates were 24%, 40%, 63%, and 65%, respectively, but no effect on thrombin-induced internal Ca2+ release from dense tubular system.
  
  
  CONCLUSIONS
  Genistein is a potential anti-platelet agent, mainly due to an inhibition of Ca2+ influx.
authors.affiliation: Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, China.
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Song
authors.forename: N C
authors.initials: NC
authors.lastname: Liang
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D019833:Genistein / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013552:Swine
chemical_list: D010975:Platelet Aggregation Inhibitors; D019833:Genistein; D002118:Calcium