Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports.
A poly-His tag was fused in the glutaryl acylase (GA) from Acinetobacter sp. strain YS114 cloned in E. coli yielding a fully active enzyme. Biochemical analyses showed that the tag did not alter the maturation of the chimeric GA (poly-His GA) that undergoes a complex post-translational processing from an inactive monomeric precursor to the active heterodimeric enzyme. This enzyme has been used as a model to develop a novel and very simple procedure for one-step purification of poly-His proteins via immobilized metal-ion affinity chromatography on tailor-made supports. It was intended to improve the selectivity of adsorption of the target protein on tailor-made chelate supports instead of performing a selective desorption. The rate and extent of the adsorption of proteins from a crude extract from E. coli and of pure poly-His tagged GA on different metal chelate supports was studied. Up to 90% of proteins from E. coli were adsorbed on commercial chelate supports having a high density of ligands attached to the support through long spacer arms, while this adsorption becomes almost negligible when using low ligand densities, short spacer arms and Zn2+ or Co2+ as cations. On the contrary, poly-His GA adsorbs strongly enough on all supports. A strong affinity interaction between the poly-His tail and a single chelate moiety seems to be the responsible for the adsorption of poly-His GA. By contrast, multipoint weak interactions involving a number of chelate moieties seem to be mainly responsible for adsorption of natural proteins. By using tailor-made affinity supports, a very simple procedure for one-step purification of GA with minimal adsorption of host proteins could be performed. Up to 20 mg of GA were adsorbed on each ml of chelate support while most of accompanying proteins were hardly adsorbed on such supports. Following few washing steps, the target enzyme was finally recovered (80% yield) by elution with 50 mM imidazole with a very high increment of specific activity (up to a 120 purification factor).
Armisén
P
P
Hispanagar S.A., Burgos, Spain.
Mateo
C
C
Cortés
E
E
Barredo
J L
JL
Salto
F
F
Diez
B
B
Rodés
L
L
García
J L
JL
Fernández-Lafuente
R
R
Guisán
J M
JM
D000150:Acinetobacter / Q000201:enzymology; D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006639:Histidine / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010399:Penicillin Amidase; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
D002614:Chelating Agents; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D006639:Histidine; D003300:Copper; D000581:Amidohydrolases; D010399:Penicillin Amidase; C046586:glutarylamidocephalosporanic acid acylase