Effect of manoalide on apoptosis induced by deprivation of growth factors in vascular endothelial cells.
OBJECTIVE
To study effect of manoalide on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells (VEC).


METHODS
Morphologic changes were observed by light microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was analyzed by agarose gel electrophoresis and fluorescence microscopy.


RESULTS
The cells deprived of aFGF and serum were exposed to manoalide 1-4 mumol. L-1 for 48 h, detachment and DNA fragmentation of these cells were suppressed. At 7 mumol. L-1, manoalide promoted detachment and DNA fragmentation of VEC.


CONCLUSIONS
manoalide 2 mumol.L-1 inhibited, but 7 mumol.L-1 promoted, apoptosis of VEC.
Miao
J Y
JY
Institute of Developmental Biology, School of Life Science, Shandong University, Ji-nan, China.
Araki
S
S
Zhang
H W
HW
Hayashi
H
H
D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D006801:Humans; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D013729:Terpenes / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D016895:Culture Media, Serum-Free; D013729:Terpenes; D016220:Fibroblast Growth Factor 1; C045673:manoalide; D010741:Phospholipases A