title: Carbonic anhydrase from Vicia canencens leaves.
abstract: Carbonic anhydrases (CA: Carbonate hydrolase; E, C,4.2.1.1) from leaves Vicia canencens were purified and were characterized. The purification level of enzyme was 76-fold. The optimum temperature of this enzyme was 70 degrees C. pH optimum was 9.2. Each of the enzyme molecules was a decamer having MW of 262,000 and the subunit MW was 26,400.
authors.affiliation: Atatürk Universitesi Eğitim Fakültesi Kimya Eğitimi Bölümü, Erzurum, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Demir
authors.forename: N
authors.initials: N
authors.lastname: Demir
authors.forename: O I
authors.initials: OI
authors.lastname: Küfrevioğlu
mesh_terms: D002021:Buffers; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology*; D013696:Temperature
chemical_list: D002021:Buffers; D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases