Identification of the receptor for Bacillus sphaericus crystal toxin in the brush border membrane of the mosquito Culex pipiens (Diptera: Culicidae).
The binary toxin (Bin) from Bacillus sphaericus crystals specifically binds to soluble midgut brush border membrane proteins from Culex pipiens larvae. A single 60 kDa midgut membrane protein is identified as the binding protein. This protein is anchored in the mosquito midgut membrane via a glycosyl-phosphatidylinositol (GPI) anchor, and is partially released by phosphatidylinositol specific-phospholipase C (PI-PLC). Fractionation of soluble proteins by anion exchange chromatography indicates that the binding protein does not co-elute with leucine aminopeptidase activity. After partial purification, the sequences of internal amino acid fragments of the 60 kDa protein were determined. The peptide sequences were compared with data in GenBank, and showed a very high degree of similarity with enzymes belonging to the alpha-amylase family. Further enzymatic investigation showed that the receptor of the Bin toxin in C. pipiens larval midgut may be an alpha-glucosidase.
Silva-Filha
M H
MH
Institut Pasteur, Paris, France.
Nielsen-LeRoux
C
C
Charles
J F
JF
D000818:Animals; D001407:Bacillus; D001427:Bacterial Toxins / Q000378:metabolism*; D002793:Cholic Acids; D002852:Chromatography, Ion Exchange; D003465:Culex / Q000201:enzymology*; D003902:Detergents; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008871:Microvilli / Q000201:enzymology; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D010738:Type C Phospholipases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000235:genetics / Q000378:metabolism*
C405322:Bacillus sphaericus binary toxin receptor; D001427:Bacterial Toxins; D002793:Cholic Acids; D003902:Detergents; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C046134:binB protein, Bacillus sphaericus; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D000520:alpha-Glucosidases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate