title: Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
abstract: The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the archaea shows low sequence identity (16-20%) with its eubacterial and eukaryotic counterparts. The crystal structure of the apo GAPDH from Sulfolobus solfataricus has been determined by multiple isomorphous replacement at 2.05 A resolution. The enzyme has several differences in secondary structure when compared with eubacterial GAPDHs, with an overall increase in the number of alpha-helices. There is a relocation of the active-site residues within the catalytic domain of the enzyme. The thermostability of the S. solfataricus enzyme can be attributed to a combination of an ion pair cluster and an intrasubunit disulphide bond.
authors.affiliation: Schools of Chemistry and Biological Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: M N
authors.initials: MN
authors.lastname: Isupov
authors.forename: T M
authors.initials: TM
authors.lastname: Fleming
authors.forename: A R
authors.initials: AR
authors.lastname: Dalby
authors.forename: G S
authors.initials: GS
authors.lastname: Crowhurst
authors.forename: P C
authors.initials: PC
authors.lastname: Bourne
authors.forename: J A
authors.initials: JA
authors.lastname: Littlechild
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D016944:Sulfolobus / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases