Analysis of apolipoproteins and lipoproteins by capillary electrophoresis.
Capillary electrophoresis provides a valuable analytical tool for the analysis of apolipoproteins and lipoproteins. Sodium dodecyl sulfate (SDS) capillary gel electrophoresis can resolve human and animal apolipoprotein (apo) A-I, apo A-II, apo E and apo A-IV in isolated high density lipoproteins (HDLs) and is capable of analysing HDL apo A-I and apo A-II content with a coefficient of variation (CV) of less than 5%. Capillary zone electrophoresis (CZE) using coated capillaries with Tricine-urea buffer can also be used for the analysis of HDL apolipoproteins and is also capable of resolving apo A-I isoforms, pro-apo A-I, mature apo A-I and deamidated apo A-I. Furthermore it can separate human A-I from endogenous rabbit A-I in transgenic rabbits expressing the human apo A-I gene. Capillary isoelectric focusing can also separate apo A-I isoforms. Analysis of charge-modified low density lipoprotein (LDL) (ox-LDL) produced by in vitro lipid peroxidation can be performed by CZE and the technique can be used to monitor simultaneous changes in the electrophoretic mobility and absorption spectra of LDL.
Stocks
J
J
Department of Cardiovascular Biochemistry, St Bartholomew's and the Royal London College of Medicine and Dentistry, UK. j.stocks@mds.qmw.ac.uk
Miller
N E
NE
D000818:Animals; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood
D001053:Apolipoproteins; D001055:Apolipoproteins B; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL