title: Laboratory-scale inactivation of African swine fever virus and swine vesicular disease virus in pig slurry.
abstract: Two methods were evaluated for the inactivation of African swine fever (ASV) and swine vesicular disease (SVD) viruses in pig slurry: chemical treatment and heat treatment. The addition of NaOH or Ca(OH)2 at different concentration/time combinations at 4 degrees C and 22 degrees C was examined, as was virus stability at different temperature/time combinations. ASF virus (ASFV) was less resistant to both methods than SVD virus (SVDV). In slurry from one source, ASFV was inactivated at 65 degrees C within 1 min, whereas SVDV required at least 2 min at 65 degrees C. However, it was found that thermal inactivation depended on the characteristics of the slurry used. Addition of 1% (w/v) of NaOH or Ca(OH)2 caused the inactivation of ASFV within 150 s at 4 degrees C; 0.5% (w/v) NaOH or Ca(OH)2 required 30 min for inactivation. NaOH or Ca(OH)2 (1% (w/v)) was not effective against SVDV at 22 degrees C after 30 min, and 1.5% (w/v) NaOH or Ca(OH)2 caused inactivation of SVDV at both 4 degrees C and 22 degrees C. At higher chemical concentrations or temperatures, ASFV and SVDV inactivation was faster in slurry than in buffered medium.
authors.affiliation: Silsoe Research Institute, Bedford, UK. claire.turner@bbsrc.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Turner
authors.forename: S M
authors.initials: SM
authors.lastname: Williams
mesh_terms: D000358:African Swine Fever Virus / Q000187:drug effects / Q000502:physiology*; D000822:Animal Husbandry; D000818:Animals; D002126:Calcium Hydroxide / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D029822:Enterovirus B, Human / Q000187:drug effects / Q000502:physiology*; D006358:Hot Temperature; D008372:Manure / Q000821:virology*; D010865:Pilot Projects; D012972:Sodium Hydroxide / Q000494:pharmacology; D013552:Swine / Q000821:virology*
chemical_list: D008372:Manure; D012972:Sodium Hydroxide; D002126:Calcium Hydroxide