Antibodies produced by mice immunized with recombinant vaccinia viruses expressing two different types of a major Theileria sergenti surface antigen (p32) react with the native surface antigen.
A 32 kDa major surface antigen, p32, of Theileria sergenti at the piroplasm stage is the main target of the host immune response. The immunogenic property of the p32 varies in some strains among the population of Theileria sergenti in Japan where the Chitose type and the Ikeda type are the most common varieties. We have constructed vaccinia virus recombinants vv/p32C and vv/p32I which harbor the Chitose and Ikeda types of p32 gene, respectively. It was found that vv/p32C and vv/p32I produced type-specific p32 which did not cross react with the monoclonal antibodies (mAbs) against the other type of p32. When mice were immunized with vv/p32C and vv/p32I, antibodies against p32 were detectable 2 weeks after the immunization, and these antibodies reacted with the native surface antigen in purified T. sergenti merozoite.
Takasima
Y
Y
Department of Global Agricultural Science, Graduate School of Agricultural and Life Science, The University of Tokyo, Japan.
Xuan
X
X
Matsumoto
Y
Y
Onuma
M
M
Otsuka
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D017931:DNA Primers / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000737:chemistry; D005260:Female; D006801:Humans; D007114:Immunization / Q000662:veterinary; D051379:Mice; D010957:Plasmids / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000737:chemistry; D016794:Theileria / Q000276:immunology*; D013801:Theileriasis / Q000517:prevention & control*; D014616:Vaccinia virus / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000954:Antigens, Surface; D017931:DNA Primers; D011994:Recombinant Proteins