Survival of Streptococcus pyogenes under stress and starvation.
The ability of Streptococcus pyogenes to enter a quiescent state, similar to the stationary phase of lab cultures, is believed to be an important factor in its ability to persist within the host and to subsequently cause disease. Using a model broth system, we determined that after entering the stationary phase, there was a 99.99% reduction in cell viability over a 4-day period, following which the cells appeared to enter a resistant starvation state where cell numbers remained constant over the subsequent 3-4 weeks. This starvation response was induced by carbon or phosphorous limitation, but not by nitrogen limitation in the form of amino acids where cells became non-culturable after 4 days. Amino acid utilization in the absence of a carbon source may be an essential factor for the long-term survival of this bacterium in the stationary phase. Early stationary phase cells showed a greater resistance to oxidative and pH stress compared to 24-h-starved cultures. There was evidence for the formation of a viable but non-culturable state as indicated by a comparison of the numbers of cells with a functional membrane potential (rhodamine 123) against culturable cells on either Todd Hewitt broth agar or sheep blood agar. Long-term survival of S. pyogenes was dependent on both cell wall and protein synthesis, suggesting that starving cultures are a dynamic cell population.
Trainor
V C
VC
Department of Microbiology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
Udy
R K
RK
Bremer
P J
PJ
Cook
G M
GM
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002244:Carbon / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D010400:Penicillin G / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D010406:Penicillins; D002701:Chloramphenicol; D002244:Carbon; D010400:Penicillin G