A simple two-dimensional high performance liquid chromatography/high performance capillary electrophoresis set-up for the separation of complex mixtures.
A two-dimensional high performance liquid chromatography/capillary electrophoresis (HPLC/CE) instrumental set-up was assembled from commercially available equipment. Fractions of the effluent from the HPLC system are collected into microtiter plates with a microfraction collector. The fractions are then dried under vacuum at room temperature, reconstituted, and analyzed by capillary zone electrophoresis (CZE). This method allows the collection of samples by time, drops, or external signal (peaks). Any size or type of HPLC or CE column can be used with no limitation on the amount of sample injected into the HPLC. Any CE detection, laser-induced fluorescence (LIF), mass spectrometry (MS), ultraviolet (UV) or other, can be used. This set-up is practical, simple, robust and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of protein digest.
Issaq
H J
HJ
SAIC Frederick, NCI-Frederick Cancer Research and Development Center, MD 21702, USA. Issaqh@mail.ncifcrf.gov
Chan
K C
KC
Janini
G M
GM
Muschik
G M
GM
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D003574:Cytochrome c Group / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D009211:Myoglobin / Q000032:analysis; D010449:Peptide Mapping / Q000379:methods*; D013997:Time Factors
D003574:Cytochrome c Group; D009211:Myoglobin