Impairment of GABA-mediated contractions of rat isolated ileum by experimental diabetes.
The effect of diabetes induced by pretreatment with streptozotocin 6 weeks prior to the study on the responses induced by gamma-aminobutyric acid (GABA), the GABA(A) agonist 3-aminopropane sulfonic acid (3-APS), and acetylcholine in the rat isolated ileum was evaluated. GABA, 3-APS, and acetylcholine showed a rightward shift in their concentration-dependent effects in the ileum that also attained a lower maximum in streptozotocin-diabetic rats as compared with control rats (p < 0.05). It is suggested that the reduced contractile responses of ileal smooth muscle to GABA and 3-APS might be due to a direct effect of diabetes on the GABAergic system or to its effect on the cholinergic system.
Ozdem
S S
SS
Department of Pharmacology, Faculty of Medicine, Akdeniz University, Antalya, Turkey. ozdem@med.akdeniz.edu.tr
Sadan
G
G
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D007082:Ileum / Q000187:drug effects* / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D005680:gamma-Aminobutyric Acid; D000109:Acetylcholine