Vitamin B status in patients with chronic fatigue syndrome.
Some patients with chronic fatigue syndrome say they benefit from taking vitamin supplements. We assessed functional status for the B vitamins pyridoxine, riboflavin and thiamine in 12 vitamin-untreated CFS patients and in 18 healthy controls matched for age and sex. Vitamin-dependent activities--aspartate aminotransferase (AST) for pyridoxine, glutathione reductase (GTR) for riboflavin, transketolase (TK) for thiamine--were measured in erythrocyte haemolysates before and after in-vitro addition of the relevant vitamin. For all three enzymes basal activity (U/g Hb) was lower in CFS patients than in controls: AST 2.84 (SD 0.62) vs 4.61 (1.43), P < 0.001; GTR 6.13 (1.89) vs 7.42 (1.25), P < 0.04; TK 0.50 (0.13) vs 0.60 (0.07), P < 0.04. This was also true of activated values: AST 4.91 (0.54) vs 7.89 (2.11), P < 0.001; GTR 8.29 (1.60) vs 10.0 (1.80), P < 0.001; TK 0.56 (0.19) vs 0.66 (0.08), P < 0.07. The activation ratios, however, did not differ between the groups. These data provide preliminary evidence of reduced functional B vitamin status, particularly of pyridoxine, in CFS patients.
Heap
L C
LC
Department of Clinical Biochemistry, King's College School of Medicine, London, UK.
Peters
T J
TJ
Wessely
S
S
D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D015673:Fatigue Syndrome, Chronic / Q000097:blood*; D005260:Female; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D011736:Pyridoxine / Q000494:pharmacology / Q000502:physiology; D012256:Riboflavin / Q000494:pharmacology / Q000502:physiology; D013831:Thiamine / Q000494:pharmacology / Q000502:physiology; D014174:Transketolase / Q000097:blood; D014803:Vitamin B Complex / Q000502:physiology*
D014803:Vitamin B Complex; D005980:Glutathione Reductase; D014174:Transketolase; D001219:Aspartate Aminotransferases; D011736:Pyridoxine; D012256:Riboflavin; D013831:Thiamine