title: Effectiveness of a manual disinfection procedure in eliminating hepatitis C virus from experimentally contaminated endoscopes.
abstract: BACKGROUND
  Transmission of hepatitis C virus (HCV) through endoscopy has been reported, but the implications as a public health concern remain controversial. This study investigated the degree to which a thorough manual cleaning-washing-disinfection procedure can decontaminate all channels of a flexible submersible endoscope experimentally contaminated with HCV.
  
  
  METHODS
  To assess the accuracy of the method currently in use, the initial investigation focused on sampling effectiveness. Nine endoscopes were contaminated with high-titer HCV-positive plasma and flushed with 150 mL of sampling solution (distilled water) before disinfection. To assess the effectiveness of the disinfection procedure, the following sequence was performed on another 10 endoscopes: inoculation, disinfection, and sampling. After concentration residual viruses were detected by means of RNA amplification with commercial assays.
  
  
  RESULTS
  The study showed that sampling alone can reduce viral titer to one-fourth its original value. Within the limits of this method, HCV RNA was never detected by means of polymerase chain reaction after disinfection, whereas all internal amplification controls were positive. This reduction to less than 1/100,000 of original titer exceeds the criterion expected for the virucidal activity of disinfectants.
  
  
  CONCLUSIONS
  The results of this in vitro experiment provided evidence that patient-to-patient endoscopic transmission HCV can be reduced, if not eliminated, with the current mechanical cleaning-washing-disinfection procedure.
authors.affiliation: Department of Virology, Faculté de Médecine, Grenoble, France.
authors.forename: B
authors.initials: B
authors.lastname: Chanzy
authors.forename: D L
authors.initials: DL
authors.lastname: Duc-Bin
authors.forename: B
authors.initials: B
authors.lastname: Rousset
authors.forename: P
authors.initials: P
authors.lastname: Morand
authors.forename: C
authors.initials: C
authors.lastname: Morel-Baccard
authors.forename: B
authors.initials: B
authors.lastname: Marchetti
authors.forename: J
authors.initials: J
authors.lastname: Fauconnier
authors.forename: M R
authors.initials: MR
authors.lastname: Mallaret
authors.forename: J
authors.initials: J
authors.lastname: Calop
authors.forename: J P
authors.initials: JP
authors.lastname: Zarski
authors.forename: J M
authors.initials: JM
authors.lastname: Seigneurin
mesh_terms: D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D019723:Endoscopes; D004866:Equipment Contamination; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics; D006526:Hepatitis C / Q000517:prevention & control* / Q000635:transmission; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis
chemical_list: D004202:Disinfectants; D012367:RNA, Viral