title: [Isolation, crystallization, and preliminary x-ray study of a Fab fragment of monoclonal antibody against human interleukin-2 and its complex with antigenic peptide].
abstract: Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human interleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelectric focusing method. Various monocrystals of these free Fab-fragments and their complexes with the antigenic peptide corresponding to the 59-72 sequence of interleukin-2 were obtained. These were shown to be suitable for X-ray and were preliminarily studied by X-ray.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Mikhaĭlova
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Mareeva
authors.forename: I N
authors.initials: IN
authors.lastname: Tsygannik
authors.forename: I I
authors.initials: II
authors.lastname: Mikhaleva
authors.forename: L V
authors.initials: LV
authors.lastname: Onoprienko
authors.forename: A A
authors.initials: AA
authors.lastname: Vikhrov
authors.forename: E A
authors.initials: EA
authors.lastname: Markvicheva
authors.forename: W
authors.initials: W
authors.lastname: Panghorn
authors.forename: W
authors.initials: W
authors.lastname: Duax
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmiianov
authors.forename: V Z
authors.initials: VZ
authors.lastname: Pletnev
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000302:isolation & purification*; D007376:Interleukin-2 / Q000276:immunology*; D007525:Isoelectric Focusing; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2; D010446:Peptide Fragments
vernacular_title: Poluchenie, kristallizatsiia i predvaritel'nye rentgenostrukturnye issledovaniia Fab-fragmenta monoklonal'nogo antitela k interleĭkinu-2 cheloveka i ego kompleksa s antigennym peptidom.