title: Gene expression analysis by transcript profiling coupled to a gene database query.
abstract: We describe an mRNA profiling technique for determining differential gene expression that utilizes, but does not require, prior knowledge of gene sequences. This method permits high-throughput reproducible detection of most expressed sequences with a sensitivity of greater than 1 part in 100,000. Gene identification by database query of a restriction endonuclease fingerprint, confirmed by competitive PCR using gene-specific oligonucleotides, facilitates gene discovery by minimizing isolation procedures. This process, called GeneCalling, was validated by analysis of the gene expression profiles of normal and hypertrophic rat hearts following in vivo pressure overload.
authors.affiliation: CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA. Rshimket@curagen.com
authors.forename: R A
authors.initials: RA
authors.lastname: Shimkets
authors.forename: D G
authors.initials: DG
authors.lastname: Lowe
authors.forename: J T
authors.initials: JT
authors.lastname: Tai
authors.forename: P
authors.initials: P
authors.lastname: Sehl
authors.forename: H
authors.initials: H
authors.lastname: Jin
authors.forename: R
authors.initials: R
authors.lastname: Yang
authors.forename: P F
authors.initials: PF
authors.lastname: Predki
authors.forename: B E
authors.initials: BE
authors.lastname: Rothberg
authors.forename: M T
authors.initials: MT
authors.lastname: Murtha
authors.forename: M E
authors.initials: ME
authors.lastname: Roth
authors.forename: S G
authors.initials: SG
authors.lastname: Shenoy
authors.forename: A
authors.initials: A
authors.lastname: Windemuth
authors.forename: J W
authors.initials: JW
authors.lastname: Simpson
authors.forename: J F
authors.initials: JF
authors.lastname: Simons
authors.forename: M P
authors.initials: MP
authors.lastname: Daley
authors.forename: S A
authors.initials: SA
authors.lastname: Gold
authors.forename: M P
authors.initials: MP
authors.lastname: McKenna
authors.forename: K
authors.initials: K
authors.lastname: Hillan
authors.forename: G T
authors.initials: GT
authors.lastname: Went
authors.forename: J M
authors.initials: JM
authors.lastname: Rothberg
mesh_terms: D000818:Animals; D006332:Cardiomegaly / Q000235:genetics / Q000473:pathology; D016208:Databases, Factual; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D012333:RNA, Messenger