title: Development of a new DNA sequencing method: 3'-ester cleavage catalyzed by Taq DNA polymerase.
abstract: A new 3'-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.
authors.affiliation: Unité de Chimie Organique, Institut Pasteur, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Rasolonjatovo
authors.forename: S R
authors.initials: SR
authors.lastname: Sarfati
mesh_terms: D002384:Catalysis; D004952:Esters; D006868:Hydrolysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism*
chemical_list: D004952:Esters; D019914:Taq Polymerase