The effect of cis-diamminedichloroplatinum, selenite and a conjugate of cisplatin with selenite [(NH3)2Pt(SeO3)] on oxidative stress in blood platelets measured by chemiluminescence method.
The effects of cisplatin, sodium selenite and a conjugate of these compounds [(NH3)2Pt(SeO3)] on the generation of free radicals in blood platelets measured by chemiluminescence method were investigated in vitro. In platelets incubated with cisplatin (20 microM, 5 min) a dose-dependent increase of chemiluminescence was observed (p < 0.05). Contrary to the stimulatory action of cisplatin (20 microM) on the production of free radicals, selenite (1 microM) and its conjugate with cisplatin (20 microM) had only slight effects on the oxidative stress in platelets (p < 0.05). The observed increase of chemiluminescence after cisplatin action correlated with a decrease of platelet free thiols present in reduced glutathione. Pre-treatment of blood platelets with buthionine sulfoximine (50 microM, 1 h, 37 degrees C) leading to a decrease of glutathione reduced the cisplatin-induced generation of free radicals in these cells (p < 0.05).
Olas
B
B
Department of Biochemistry, Institute of Biochemistry, University of Lódź, Poland. olasb@biol.uni.lodz.pl
Zbikowska
H M
HM
Wachowicz
B
B
Buczyński
A
A
Krajewski
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002945:Cisplatin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003603:Cytotoxins / Q000494:pharmacology*; D005609:Free Radicals; D008163:Luminescent Measurements; D018384:Oxidative Stress / Q000187:drug effects*; D018038:Sodium Selenite / Q000494:pharmacology*; D013552:Swine
D000970:Antineoplastic Agents; D003603:Cytotoxins; D005609:Free Radicals; D018038:Sodium Selenite; D002945:Cisplatin