Peculiarities of production technology and properties of natural animal alpha-interferon preparations.
Principally new biotechnology of production of natural animal alpha-IFNs was created. Normative technical documentation on industrial production of these preparations was transmitted to the plants of Ukraine for their industrial obtaining. The peculiarity of proposed technology consists in usage of non-traditional raw-material--lymphoid organs and tissues (perhaps spleen), and also in introduction of coinduction into industrial cycle with the help of camizole (compound of phenilimidazothiazole family) which increased the output of final product by 3-8 times. Obtained preparations were tested in vitro and in vivo experiments. As it was shown in both cases bactericidal and absorbed activity of monocytes and neutrophils increased by 2-5 times, antibody genesis sharply increased after immunization of pigs and calves by colibacteriosis vaccine: titers were higher by 3-8 times (comparatively with control), especially two month after revaccination, but they were more stable too. Fractions of 12 and 18 kDa responsible for antibacterial and antitoxic activity respectively were found in non-purified preparations of alpha-IFNs.
Kishko
Ia H
IaH
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
D000818:Animals; D000831:Animals, Newborn; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000907:Antibodies, Bacterial / Q000097:blood / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D001709:Biotechnology / Q000379:methods*; D002417:Cattle; D004926:Escherichia coli / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D013552:Swine; D014455:Ukraine
D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D016898:Interferon-alpha