Preparation and characterization of protein-loaded poly(epsilon-caprolactone) microparticles for oral vaccine delivery.
This paper describes the conditions of preparation of poly(epsilon-caprolactone) (PCL) microparticles with a mean size between 5 and 10 microm, obtained by a double emulsion-solvent evaporation technique, suitable for oral vaccine delivery. Bovine serum albumin (BSA) was used as water-soluble model antigen for encapsulation. Different parameters influencing the microparticle size, the BSA loading and entrapment efficiency were investigated. Spherical, smooth and homogeneously distributed microparticles were produced with a BSA loading and entrapment efficiency reaching, respectively, 5% (w/w) and 30%. Polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF) analyses of BSA released from these particles confirmed that the entrapped protein seemed to remain unaltered by the protein encapsulation process.
Benoit
M A
MA
Université Catholique de Louvain, Ecole de Pharmacie, Laboratoire de Pharmacie Galénique, Industrielle et Officinale, Avenue Emmanuel Mounier 73.20, 1200, Brussels, Belgium. benoit@farg.ucl.ac.be
Baras
B
B
Gillard
J
J
D000284:Administration, Oral; D002626:Chemistry, Pharmaceutical; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D005079:Excipients / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry*; D014783:Viscosity; D014867:Water / Q000737:chemistry
D004655:Emulsions; D005079:Excipients; D011091:Polyesters; D012996:Solutions; D014612:Vaccines; D014867:Water; C016240:polycaprolactone; D012710:Serum Albumin, Bovine