Bacteriophage PRD1 contains a labile receptor-binding structure at each vertex.
Bacteriophage PRD1 is a membrane-containing virus with an unexpected similarity to adenovirus. We mutagenized unassigned PRD1 genes to identify minor capsid proteins that could be structural or functional analogs to adenovirus proteins. We report here the identification of an amber mutant, sus525, in an essential PRD1 gene XXXI. The gene was cloned and the gene product was overexpressed and purified to near homogeneity. Analytical ultracentrifugation and gel filtration showed that P31 is a homopentamer of about 70 kDa. The protein was shown to be accessible on the virion surface and its absence in the sus525 particles led to the deficiency of two other viral coat proteins, protein P5 and the adsorption protein P2. Cryo-electron microscopy and image reconstruction of the sus525 particles indicate that these proteins are located on the capsid vertices, because in these particles the entire vertex structure was missing along with the peripentonal major capsid protein P3 trimers. Sus525 particles package DNA effectively but loose it upon purification. All of the PRD1 vertex structures are labile and potentially capable of mediating DNA delivery; this is in contrast to other dsDNA phages which employ a single vertex for packaging and delivery. We propose that this arises from a symmetry mismatch between protein P2 and the pentameric P31 in analogy to that between the adenovirus penton base and the receptor-binding spike.
Rydman
P S
PS
Department of Biosciences and Institute of Biotechnology Viikki Biocenter, University of Helsinki, Helsinki, FIN-00014, Finland.
Caldentey
J
J
Butcher
S J
SJ
Fuller
S D
SD
Rutten
T
T
Bamford
D H
DH
D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D001665:Binding Sites; D002213:Capsid / Q000737:chemistry* / Q000235:genetics* / Q000648:ultrastructure; D004279:DNA, Viral / Q000737:chemistry; D005814:Genes, Viral; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D013045:Species Specificity; D017916:Tectiviridae / Q000737:chemistry* / Q000235:genetics* / Q000648:ultrastructure
D004279:DNA, Viral; D011994:Recombinant Proteins