title: Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype inaba biotype E1 Tor and assessment of its reactogenicity and immunogenicity in the rabbit model.
abstract: The disease cholera is an important cause of mortality in many developing countries. Though it can be controlled through improved sanitation, this goal is not easily attainable in many countries. Development of an efficacious vaccine offers the best immediate solution. A new oral candidate vaccine has been constructed from a non-toxigenic strain of Vibrio cholerae E1 Tor, Inaba, which is not only devoid of the cholera toxin (CT) virulence cassette but also is completely non-reactogenic in rabbit ileal loop assay. The strain, however, had toxR and tcpA genes. Through a series of manipulations, the ctxB gene of V. cholerae, responsible for the production of the 'B' subunit of the cholera toxin (CTB) was introduced into the cryptic hemolysin locus of the strain. The resulting strain, named vaccine attempt 1.3 (VA1.3), was found to be able to produce copious amounts of CTB. In the RITARD model this strain was found to be non-reactogenic and provided full protection against the challenge doses of both V. cholerae O1, classical and E1 Tor. In the immunized rabbit it invoked significant levels of anti-bacterial and anti-toxin immunity.
authors.affiliation: Institute of Microbial Technology, Sector 39A, Chandigarh, India.
authors.forename: M
authors.initials: M
authors.lastname: Thungapathra
authors.forename: C
authors.initials: C
authors.lastname: Sharma
authors.forename: N
authors.initials: N
authors.lastname: Gupta
authors.forename: R K
authors.initials: RK
authors.lastname: Ghosh
authors.forename: A
authors.initials: A
authors.lastname: Mukhopadhyay
authors.forename: H
authors.initials: H
authors.lastname: Koley
authors.forename: G B
authors.initials: GB
authors.lastname: Nair
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015373:Bacterial Typing Techniques; D002771:Cholera / Q000517:prevention & control; D002772:Cholera Toxin / Q000235:genetics; D022121:Cholera Vaccines / Q000276:immunology*; D015195:Drug Design; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics; D008297:Male; D019081:O Antigens; D011817:Rabbits; D012703:Serotyping; D014614:Vaccines, Synthetic / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D022121:Cholera Vaccines; D006460:Hemolysin Proteins; D019081:O Antigens; D014614:Vaccines, Synthetic; D002772:Cholera Toxin