title: Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively.
abstract: The assembly of the polyketide backbone of rifamycin B on the type I rifamycin polyketide synthase (PKS), encoded by the rifA-rifE genes, is terminated by the product of the rifF gene, an amide synthase that releases the completed undecaketide as its macrocyclic lactam. Inactivation of rifF gives a rifamycin B nonproducing mutant that still accumulates a series of linear polyketides ranging from the tetra- to a decaketide, also detected in the wild type, demonstrating that the PKS operates in a processive manner. Disruptions of the rifD module 8 and rifE module 9 and module 10 genes also result in accumulation of such linear polyketides as a consequence of premature termination of polyketide assembly. Whereas the tetraketide carries an unmodified aromatic chromophore, the penta- through decaketides have undergone oxidative cyclization to the naphthoquinone, suggesting that this modification occurs during, not after, PKS assembly. The structure of one of the accumulated compounds together with (18)O experiments suggests that this oxidative cyclization produces an 8-hydroxy-7, 8-dihydronaphthoquinone structure that, after the stage of proansamycin X, is dehydrogenated to an 8-hydroxynaphthoquinone.
authors.affiliation: Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA.
authors.forename: T W
authors.initials: TW
authors.lastname: Yu
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.forename: Y
authors.initials: Y
authors.lastname: Doi-Katayama
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.forename: C
authors.initials: C
authors.lastname: Park
authors.forename: B S
authors.initials: BS
authors.lastname: Moore
authors.forename: C
authors.initials: C
authors.lastname: Richard Hutchinson
authors.forename: H G
authors.initials: HG
authors.lastname: Floss
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism*; D016296:Mutagenesis; D015183:Restriction Mapping; D012294:Rifamycins / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D012294:Rifamycins