title: [A study on polymorphism of mitochondrial DNA D loop in the Han nationality in China].
abstract: OBJECTIVE
  To investigate the polymorphism of mitochondrial DNA D loop in the Han nationality in China and the mean nucleic acid substitute frequency on each site.
  
  
  METHODS
  A 465bp-long DNA fragment in mtDNA D loop was amplified by PCR technique and the polymorphism was analyzed by restriction fragment length polymorphism (RFLP) using 6 restriction enzymes (on 10 sites). Some samples were analyzed by direct sequencing of PCR products.
  
  
  RESULTS
  RFLP analyses with 6 restriction enzymes on the 10 sites generated 14 restriction types. The calculated mean nucleic acid substitute frequency (based on Nei formula) on each site of mtDNA D loop in the Han nationality in China was 0.029.
  
  
  CONCLUSIONS
  The mean nucleic acid substitute frequency on each site in the D loop region was 10 times as high as that in mtDNA.
authors.affiliation: Department of Biology, Harbin Medical University, Harbin, 150086 P.R. China. zhusj@ems.hrbmu.edu.cn
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Feng
authors.forename: S
authors.initials: S
authors.lastname: Fu
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial