LTB(4)-induced nasal gland serous cell secretion mediated by neutrophil elastase.
Local allergen challenge causes nasal hypersecretion and also causes local leukotriene (LT) release, including LTB(4). Because LTB(4) causes leukocyte recruitment, and because neutrophil elastase is a potent secretagogue, we examined the hypothesis that LTB(4) causes nasal hypersecretion via neutrophil elastase. We developed a method for isolating and superfusing a nasal segment in dogs. Instillation of LTB(4) into the nasal segment caused a time-dependent increase in the volume of airway fluid, in lysozyme secretion, and in the recruitment of neutrophils. ICI 200,355, a selective inhibitor of neutrophil elastase, prevented LTB(4)-induced nasal secretion and lysozyme secretion, but it had no effect on neutrophil recruitment. We conclude that LTB(4) causes potent nasal secretion via release of elastase, and therefore LTB(4) may play a major role in allergic nasal hypersecretion.
Cardell
L O
LO
Cardiovascular Research Institute and Department of Medicine, University of California San Francisco, San Francisco, California, USA.
Agustí
C
C
Takeyama
K
K
Stjärne
P
P
Nadel
J A
JA
D000818:Animals; D004285:Dogs; D007975:Leukotriene B4 / Q000494:pharmacology*; D009113:Muramidase / Q000378:metabolism; D009297:Nasal Mucosa / Q000187:drug effects* / Q000378:metabolism; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D009842:Oligopeptides / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000502:physiology*; D012636:Secretory Rate / Q000187:drug effects; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology
D009842:Oligopeptides; D015842:Serine Proteinase Inhibitors; C069366:ICI 200355; D007975:Leukotriene B4; D009113:Muramidase; D010196:Pancreatic Elastase