title: Determination of busulfan in human plasma using high-performance liquid chromatography with pre-column derivatization and fluorescence detection.
abstract: A rapid, sensitive and reproducible high-performance liquid chromatographic assay for busulfan in human plasma was developed. After extraction of plasma samples with acetonitrile and methylene chloride, busulfan and the internal standard [1,5-bis(methanesulfonyloxy)pentane] were derivatized with 8-mercaptoquinoline to yield fluorescent compounds which were detected with a fluorescence detector equipped with filters of 360 nm (excitation) and 425 nm (emission). Calibration graphs showed a linear correlation (r>0.9990) over the concentration range of 20-2000 ng/ml. The recovery of busulfan from plasma standards was 70+/-5%. The detection and quantification limits for busulfan in plasma samples were established at 9 ng/ml and 20 ng/ml, respectively. The intra- and inter-assay variations were lower than 8% and 10%, respectively. The applicability of the method was verified by analyzing the plasma concentrations of busulfan in a patient to whom it was administered orally on two different days.
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Valencia, Spain.
authors.forename: J E
authors.initials: JE
authors.lastname: Peris
authors.forename: J A
authors.initials: JA
authors.lastname: Latorre
authors.forename: V
authors.initials: V
authors.lastname: Castel
authors.forename: A
authors.initials: A
authors.lastname: Verdeguer
authors.forename: S
authors.initials: S
authors.lastname: Esteve
authors.forename: F
authors.initials: F
authors.lastname: Torres-Molina
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000097:blood* / Q000493:pharmacokinetics; D002066:Busulfan / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D018906:Antineoplastic Agents, Alkylating; D002066:Busulfan