title: Characterization of the rhp7(+) and rhp16(+) genes in Schizosaccharomyces pombe.
abstract: The global genome repair (GGR) subpathway of nucleotide excision repair (NER) is capable of removing lesions throughout the genome. In Saccharomyces cerevisiae the RAD7 and RAD16 genes are essential for GGR. Here we identify rhp7 (+), the RAD7 homolog in Schizosaccharomyces pombe. Surprisingly, rhp7 (+)and the previously cloned rhp16 (+)are located very close together and are transcribed in opposite directions. Upon UV irradiation both genes are induced, reaching a maximum level after 45-60 min. These observations suggest that the genes are co-regulated. Schizo-saccharomyces pombe rhp7 or rhp16 deficient cells are, in contrast to S.cerevisiae rad7 and rad16 mutants, not sensitive to UV irradiation. In S.pombe an alternative repair mechanism, UV damage repair (UVDR), is capable of efficiently removing photolesions from DNA. In the absence of this UVDR pathway both rhp7 and rhp16 deficient cells display an enhanced UV sensitivity. Epistatic analyses show that rhp7 (+)and rhp16 (+)are only involved in NER. Repair analyses at nucleotide resolution demonstrate that both Rhp7 and Rhp16, probably acting in a complex, are essential for GGR in S.pombe.
authors.affiliation: Medical Genetics Centre South-West Netherlands, Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, 2300 RA Leiden, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Lombaerts
authors.forename: P H
authors.initials: PH
authors.lastname: Peltola
authors.forename: R
authors.initials: R
authors.lastname: Visse
authors.forename: H
authors.initials: H
authors.lastname: den Dulk
authors.forename: J A
authors.initials: JA
authors.lastname: Brandsma
authors.forename: J
authors.initials: J
authors.lastname: Brouwer
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins; D004307:Dose-Response Relationship, Radiation; D005656:Fungal Proteins / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C063915:RAD7 protein, S cerevisiae; C101945:RHP16 protein, S pombe; C121843:RHP7 protein, S pombe; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins