Determination of a new naphthoquinone derivative, NQ12, in human plasma and urine by high-performance liquid chromatography.
A high-performance liquid chromatographic method was developed for the determination of a new phospholipase A2 inhibitor, NQ12, in human plasma and urine. The sample preparation was simple: 2 volumes of acetonitrile were added to the biological samples to deproteinize it. A 50-microl aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was 0.05M acetate buffer (pH 3) : acetonitrile : methanol (30:45:25, v/v/v) and run at a flow rate of 1.5 ml/min. The column effluent was monitored by a UV detector set at 298 nm. The retention times for NQ12 and the internal standard were approximately 5.5 and 7.0 min, respectively. The detection limits for NQ12 in human plasma and urine were all 20 ng/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 11.8%) for human plasma and urine. No interferences from endogenous substances were found.
Kim
S H
SH
College of Pharmacy, Seoul National University, Kwanak-Gu, South Korea.
Park
J M
JM
Ryu
C K
CK
Lee
M G
MG
D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D009281:Naphthalenes / Q000032:analysis* / Q000097:blood / Q000652:urine; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
C403720:2-chloro-3-((4-ethyl-phenyl)-amino)-1,4-naphthalendione; D004791:Enzyme Inhibitors; D009281:Naphthalenes; D010741:Phospholipases A; D054467:Phospholipases A2