Inhibition of MT-450 rat mammary tumour growth by antibodies recognising subtypes of blood group antigen B.
Using subtractive immunization to identify cell surface epitopes expressed in a metastasis-specific fashion on cells of the rat MT-W9 mammary carcinoma model, we generated a monoclonal antibody called M-N#1. This antibody binds specifically to metastasizing cells of the MT-W9 series and also to certain other metastasizing rat mammary carcinoma cell lines. We demonstrate that the M-N#1 antibody recognizes a fucosylated N-glycosyl sugar modification, and furthermore show that the epitope specificity of the M-N#1 antibody is for blood group antigen B subtypes 2, 3 and 4 with slight cross-reactivity with blood group antigen A subtype 2. The expression of these carbohydrate epitopes on MT-450 cells is functionally important, because the M-N#1 antibody efficiently inhibits MT-450 tumour growth in spontaneous metastasis assays. These results suggest that expression of the subtypes of blood group antigen B recognized by the M-N#1 antibody does not directly participate in the metastatic cascade but rather confers a growth or survival advantage on the tumour cells.
Sleeman
J P
JP
Forschungszentrum Karlsruhe, Institute of Genetics, Germany.
Kim
U
U
LePendu
J
J
Howells
N
N
Coquerelle
T
T
Ponta
H
H
Herrlich
P
P
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002455:Cell Division; D002460:Cell Line; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D009362:Neoplasm Metastasis; D051381:Rats; D011920:Rats, Inbred WF; D014407:Tumor Cells, Cultured
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal