Determination of catecholamines in human plasma by high-performance liquid chromatography with electrochemical detection.
In the present study, assays were improved for the determination of catecholamines in human plasma. High-performance liquid chromatography with electrochemical detection was employed for quantitative analysis. The influence of various parameters on chromatographic performance, such as the composition and the pH of the mobile phase, and the detection potential, was investigated. An accurate solid-phase extraction procedure, after catecholamine complexation with diphenylborate, was developed. The efficiency yield for all catecholamines was in the range 92-98%. Relative standard deviation values for repeatability and for intermediate precision were less than 2% and 3%, respectively, for all three analytes.
Raggi
M A
MA
Department of Pharmaceutical Sciences, University of Bologna, Italy. raggima@alma.unibo.it
Sabbioni
C
C
Casamenti
G
G
Gerra
G
G
Calonghi
N
N
Masotti
L
L
D002138:Calibration; D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002395:Catecholamines