Genomic organization and chromosome location of the rat CD3zeta locus (Cd3z).
The structure and pattern of expression of the CD3zeta chain have apparently been conserved throughout mammalian evolution. The organization of the rat CD3zeta locus was determined by genomic cloning and nucleotide sequencing. Most of the rat CD3zeta coding region was similar to mouse and human CD3zeta sequences. Whereas the 3' region involving alternative splicing was relatively well conserved at the nucleotide level, the deduced amino acid sequences were different in rats, mice and humans due to the presence of deletion and insertion mutations. Alternative splicing products of the CD3zeta locus, such as CD3eta, CD3&theta; and CD3tau, which have been reported for mice, were not expressed by rat T cells. By using fluorescence in situ hybridization, we have localized rat CD3zeta to chromosome 13q22-->q23.
Matsuura
A
A
Department of Pathology, School of Medicine, Sapporo Medical University, Sapporo, Japan. amatsuur@sapmed.ac.jp
Kinebuchi
M
M
Itoh
Y
Y
Kasai
K
K
Yamada
K
K
Yoshida
M C
MC
Horie
M
M
Kikuchi
K
K
Sato
N
N
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D017252:CD3 Complex / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D005091:Exons; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D017252:CD3 Complex; C121704:CD3 antigen, zeta chain