The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities.
The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting.
Freimoser
F M
FM
Institut fr Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
Jakob
C A
CA
Aebi
M
M
Tuor
U
U
D015169:Colony Count, Microbial / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D003470:Culture Media; D020091:Entomophthorales / Q000254:growth & development / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D009172:Mycology / Q000379:methods; D013778:Tetrazolium Salts; D013844:Thiazoles
D003470:Culture Media; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue