Identification of copper-zinc superoxide dismutase gene from enteroaggregative Escherichia coli.
We describe here the identification of sodC gene from enteroaggregative Escherichia coli (EAggEC). A 294 bp gene-specific fragment was amplified from the organism by DNA as well as RT-PCR using primers from bacterial sodC sequences. The metal co-factor present in the protein was confirmed by running samples in native gels and inhibiting with 2 mM potassium cyanide. However, the nonpathogenic E. coli possesses the gene but does not express it. Thus, the presence of copper-zinc superoxide dismutase encoded by sodC was demonstrated for the first time in EAggEC, which means it could be a novel candidate for a virulence marker.
Kumar
R
R
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Sidhu
M K
MK
Ganguly
N K
NK
Chakraborti
A
A
D003300:Copper; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008667:Metalloproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics*; D015032:Zinc
D017931:DNA Primers; D029968:Escherichia coli Proteins; D008667:Metalloproteins; D003300:Copper; D013482:Superoxide Dismutase; C116114:sodC protein, E coli; D015032:Zinc