title: [Cathepsin L from human brain tumor. Purification and contents].
abstract: Cysteine cathepsin L was isolated and purified 1242-fold from human brain. Cathepsin L pH-optimum, Vmax, K(m), lysosomal origin and thiol nature were determined. The distribution of cathepsin L activity in human brain tumours of different histological structure, histogenesis and degree of malignization was studied. The activity of cathepsin L in most the tumours was found to be higher compared to the normal brain tissue.
authors.affiliation: Donezk State University, Ukraine.
authors.forename: V I
authors.initials: VI
authors.lastname: Chernaia
mesh_terms: D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D056668:Cathepsin L; D002403:Cathepsins / Q000302:isolation & purification*; D003546:Cysteine Endopeptidases / Q000302:isolation & purification*; D010450:Endopeptidases; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000201:enzymology; D009367:Neoplasm Staging
chemical_list: D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L
vernacular_title: Katepsin L iz opukholi mozga cheloveka. Ochistka i soderzhanie.