TEM-24 produced by four different species of Enterobacteriaceae, including Providencia rettgeri, in a single patient.
Four species of members of the family Enterobacteriaceae harboring extended-spectrum beta-lactamase (ESBL) were recovered in a single patient hospitalized in an intensive care unit. Among these isolates, we describe for the first time an ESBL-producing Providencia rettgeri strain. Bacteria from the same species were shown to be genetically related by pulsed-field gel electrophoresis analysis. These strains produced the same TEM derivative ESBL, characterized as TEM-24. This enzyme had the peculiarity of being encoded by a large conjugative plasmid of 180 kb, never previously described for such an ESBL.
Marchandin
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Service de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5, France. h=marchandin@chu-montpellier.fr
Carriere
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Sirot
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Pierre
H J
HJ
Darbas
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D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins; D018550:Cephalosporin Resistance; D004269:DNA, Bacterial / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000201:enzymology* / Q000382:microbiology*; D006801:Humans; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011532:Providencia / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D047090:beta-Lactams; C121000:TEM24 beta-lactamase; D001618:beta-Lactamases