Synthesis of a new analog of thymidine for in vivo non-radioactive labeling of DNA.
The introduction of 6-(p-bromobenzoylamino)caproyl radical in the methyl group of 2'-O-deoxythymidine is described. In vivo incorporation of this nucleoside to DNA was determined using a monoclonal antibody that recognized the radical.
Rodríguez-Tanty
C
C
Organic Chemistry Department, National Center for Scientific Research, Havana, Cuba.
Pérez
R
R
Miranda
J
J
Vélez-Castro
H
H
Rosado
A
A
Macías
A
A
Galán
L
L
Higginson-Clarke
D
D
Riverón
A M
AM
D004269:DNA, Bacterial / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013936:Thymidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis
D004269:DNA, Bacterial; D013936:Thymidine