Fluorescence property of oxazole yellow-linked oligonucleotide. Triple helix formation and photocleavage of double-stranded DNA in the presence of spermine.
Oxazole yellow is an intercalator that shows enhanced fluorescence upon binding to DNA. We prepared an oxazole yellow-linked oligonucleotide that can form triple helix with interleukin 2 receptor alpha chain promoter. The oxazole yellow-linked oligonucleotide showed linear increase of fluorescence by the triple helix formation with double-stranded DNA and also induced photocleavage of the targeted DNA in the presence of spermine upon visible illumination. Cleavage site of one strand was 7 or 8 bases away from the site of intercalation whereas the other strand was cleaved at the intercalated site.
Inoue
T
T
Institute for Chemical Research, Kyoto University, Uji, Japan.
Sugiura
Y
Y
Saitoh
J
J
Ishiguro
T
T
Otsuka
M
M
D001583:Benzoxazoles / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D007364:Intercalating Agents / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D011804:Quinolines / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013096:Spermine / Q000737:chemistry*
D001583:Benzoxazoles; D007364:Intercalating Agents; D009841:Oligonucleotides; D011804:Quinolines; C097015:oxazole yellow; C051802:triplex DNA; D013096:Spermine; D004247:DNA