Aqueous trifluorethanol solutions simulate the environment of DNA in the crystalline state.
We took 28 fragments of DNA whose crystal structures were known and used CD spectroscopy to search for conditions stabilising the crystal structures in solution. All 28 fragments switched into their crystal structures in 60-80% aqueous trifluorethanol (TFE) to indicate that the crystals affected the conformation of DNA like the concentrated TFE. The fragments crystallising in the B-form also underwent cooperative TFE-induced changes that took place within the wide family of B-form structures, suggesting that the aqueous and crystal B-forms differed as well. Spermine and magnesium or calcium cations, which were contained in the crystallisation buffers, promoted or suppressed the TFE-induced changes of several fragments to indicate that the crystallisation agents can decide which of the possible structures is adopted by the DNA fragment in the crystal.
Kypr
J
J
Academy of Sciences of the Czech Republic, Institute of Biophysics, Královopolská 135, CZ-61265 Brno, Czech Republic.
Chládková
J
J
Zimulová
M
M
Vorlícková
M
M
D001483:Base Sequence; D002942:Circular Dichroism; D018360:Crystallography, X-Ray / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012996:Solutions / Q000737:chemistry*; D014270:Trifluoroethanol / Q000737:chemistry*; D014867:Water / Q000737:chemistry / Q000378:metabolism
D012996:Solutions; D014867:Water; D014270:Trifluoroethanol; D004247:DNA