Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors.
Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell-cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.
Hanke
T
T
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Takizawa
H
H
McMahon
C W
CW
Busch
D H
DH
Pamer
E G
EG
Miller
J D
JD
Altman
J D
JD
Liu
Y
Y
Cado
D
D
Lemonnier
F A
FA
Bjorkman
P J
PJ
Raulet
D H
DH
D000483:Alleles; D000818:Animals; D000950:Antigens, Ly; D002352:Carrier Proteins / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000378:metabolism* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism*; D037181:Lectins, C-Type; D007959:Lymphocyte Culture Test, Mixed; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D020297:Mice, Congenic; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D055630:Receptors, NK Cell Lectin-Like; D012995:Solubility
D000950:Antigens, Ly; D002352:Carrier Proteins; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D015395:Histocompatibility Antigens Class I; D007166:Immunosuppressive Agents; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; D011971:Receptors, Immunologic; D055630:Receptors, NK Cell Lectin-Like