title: Vaccination with pertussis toxin alters the antibody response to simultaneous respiratory syncytial virus challenge.
abstract: Many bacterial toxins, including pertussis toxin (PT), exert potent adjuvant effects on antibody synthesis to coadministered antigens. In these studies, we examined whether locally or peripherally administered PT similarly altered the antibody isotype selection to replicating virus after intranasal (inl) challenge. Mice primed intramuscularly with PT and inl with respiratory syncytial virus (RSV) produced RSV-specific antibodies of the IgG2a isotype at a level similar to that of unprimed controls, with some increase in IgG1 production. Mice primed inl with both PT and RSV showed elevated RSV-specific IgG1, increased serum IgE levels, and increased interleukin (IL)-4 in lung supernatants. Splenocytes from these animals produced increased IL-4 when stimulated in vitro with RSV or PT antigens after infection. These results suggest that PT can influence the local production of IL-4 to alter the humoral and cellular immune responses to viral infection as well as to coadministered antigens.
authors.affiliation: Department of Microbiology, Vanderbilt University, Nashville, Tennessee 37232-2582, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Fischer
authors.forename: T R
authors.initials: TR
authors.lastname: Johnson
authors.forename: R S
authors.initials: RS
authors.lastname: Peebles
authors.forename: B S
authors.initials: BS
authors.lastname: Graham
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000917:Antibody Formation / Q000187:drug effects; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis* / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology* / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000276:immunology* / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000914:Antibodies, Viral; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin