Prevalence of Epstein-Barr virus in oral squamous cell carcinoma.
Forty-six samples of oral squamous cell carcinoma (OSCC) were evaluated for the prevalence of Epstein-Barr virus (EBV) infection by the polymerase chain reaction (PCR), Southern blot hybridization, and in situ hybridization (ISH). EBV DNA was detected in 7 (15.2 per cent) out of 46 samples by a combination of PCR and Southern blot hybridization methods. All seven positive samples showed well-differentiated carcinoma, thus suggesting a possible relationship between EBV infection and the degree of differentiation of carcinoma tissue. Latent infection membrane protein 1 (LMP1) was detected immunohistochemically in six of the EBV-positive OSCCs. However, no signal of the EBV-encoded small RNA (EBER)-1 was demonstrated by the ISH method. No significant relationship was observed between EBV infection and lymph node metastasis. A follow-up study (range from 4.4 to 79 months; mean 34.9 months) showed no recurrence or death to occur in the EBV-positive patients, which thus suggested a good prognosis for EBV-positive OSCC patients.
Kobayashi
I
I
Department of Oral Pathology, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Shima
K
K
Saito
I
I
Kiyoshima
T
T
Matsuo
K
K
Ozeki
S
S
Ohishi
M
M
Sakai
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D002610:Cheek; D016009:Chi-Square Distribution; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D005887:Gingival Neoplasms / Q000473:pathology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009060:Mouth Floor; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000473:pathology / Q000821:virology*; D010157:Palatal Neoplasms / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011379:Prognosis; D012367:RNA, Viral / Q000032:analysis; D014062:Tongue Neoplasms / Q000473:pathology / Q000821:virology; D014763:Viral Matrix Proteins / Q000032:analysis
D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral; D014763:Viral Matrix Proteins