title: Evaluation of cytokine production and phagocytic activity in mice infected with Campylobacter jejuni.
abstract: The effect of several Campylobacter jejuni strains on the immune response was analyzed in mice after intraperitoneal inoculation with 10(10) colony forming units (CFU). Three C. jejuni strains were assayed: CCUG 6968 (enterotoxigenic), CCUG 7580 (enterotoxigenic), and CCUG 7440 (non-enterotoxigenic). These C. jejuni strains induced a peritoneal inflammatory response and an important increase in the peritoneal phagocyte oxidative activity measured by chemiluminescence assay, as well as an increase in the number of peritoneal cells. Both interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) production by peritoneal cells were not modified. However, C. jejuni 7440 caused a statistically significant increase in TNFalpha production. These results have demonstrated that different strains of C. jejuni induce an increase of the inflammatory response without a significant cytokine release. However, these infectious microorganisms may be eliminated efficiently by murine macrophages after phagocytosis.
authors.affiliation: University of Jaén, Department of Health Sciences, Unit of Microbiology, 23071 Jaén, Spain.
authors.forename: P L
authors.initials: PL
authors.lastname: Pancorbo
authors.forename: M A
authors.initials: MA
authors.lastname: de Pablo
authors.forename: E
authors.initials: E
authors.lastname: Ortega
authors.forename: A M
authors.initials: AM
authors.lastname: Gallego
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.forename: G
authors.initials: G
authors.lastname: Alvarez de Cienfuegos
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D016466:CHO Cells / Q000187:drug effects; D002169:Campylobacter Infections / Q000276:immunology* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000276:immunology*; D002452:Cell Count; D006224:Cricetinae; D016207:Cytokines / Q000378:metabolism*; D004768:Enterotoxins / Q000378:metabolism; D006651:Histocytochemistry; D007375:Interleukin-1 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D004768:Enterotoxins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha