title: Growth inhibitory effects of somatostatin on human leukemia cell lines mediated by somatostatin receptor subtype 1.
abstract: Reverse transcription polymerase chain reaction analysis revealed that only somatostatin receptor (SSTR) 1 mRNA was expressed in Ball-1 B-, Jurkat T-, and HL60 leukemia cell lines. In contrast, human normal mononuclear cells expressed the mRNA of all five subtypes of SSTR, although the expression level of SSTR1 was the highest. A binding study, revealed that [125I]-somatostatin bound specifically to HL60 cells and this binding was inhibited concentration-dependently by unlabeled somatostatin (SS). A [3H]thymidine incorporation study showed that SS significantly and concentration-dependently inhibited HL60 and BALL-1 leukemia cell growth. Furthermore, this inhibition of leukemia cell growth was associated with reduces c-fos gene expression. These data indicate that leukemia cells express SSTR1 and SS reduce c-fos gene expression with resultant suppression of leukemia cell growth, possibly mediated by the SSTRI.
authors.affiliation: 2nd Division of Internal Medicine, Shimane Medical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ishihara
authors.forename: S
authors.initials: S
authors.lastname: Hassan
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: N
authors.initials: N
authors.lastname: Moriyama
authors.forename: R
authors.initials: R
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
authors.forename: A
authors.initials: A
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Chiba
mesh_terms: D002455:Cell Division / Q000187:drug effects; D018922:HL-60 Cells; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D017481:Receptors, Somatostatin / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D006727:Hormone Antagonists; D017481:Receptors, Somatostatin; D013004:Somatostatin