A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury.
The time-dependent inflammatory cell reaction in human cortical contusions has been investigated during the first 30 weeks after blunt head injury. Immunohistochemical staining was carried out using CD 15 for granulocytes and LCA, CD 3 and UCHL-1 for mononuclear leucocytes. In order to provide reliable data for a forensic wound age estimation, the intensity of the cellular reaction was evaluated with a quantitative image analysis system. CD 15-labelled granulocytes were detectable earliest 10 min after brain injury, whereas significantly increased numbers of mononuclear leucocytes occurred in cortical contusions after a postinfliction interval of at least 1.1 days (LCA), 2 days (CD 3) or 3.7 days (UCHL-1), respectively.
Hausmann
R
R
Department of Legal Medicine, University of Erlangen-Nürnberg, Erlangen, Germany.
Kaiser
A
A
Lang
C
C
Bohnert
M
M
Betz
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001924:Brain Concussion / Q000276:immunology* / Q000473:pathology; D017252:CD3 Complex / Q000032:analysis; D002540:Cerebral Cortex / Q000276:immunology / Q000293:injuries* / Q000473:pathology; D002648:Child; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D018836:Inflammation Mediators / Q000032:analysis*; D017493:Leukocyte Common Antigens / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000473:pathology; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D011180:Postmortem Changes; D020033:Protein Isoforms / Q000032:analysis
D017252:CD3 Complex; D018836:Inflammation Mediators; D016256:Lewis X Antigen; D020033:Protein Isoforms; D017493:Leukocyte Common Antigens