Hydrolysis of some mRNA 5'-cap analogs catalyzed by the human Fhit protein--and lupin ApppA hydrolases.
Hydrolysis of the following four cap analogs: m7G(5')ppp(5')A, m7G(5')ppp(5')m6A, m7G(5')ppp(5')m2'OG and m7G(5')ppp(5')2'dG catalyzed by homogeneous human Fhit protein and yellow lupin Ap3A hydrolase has been investigated. The hydrolysis products were identified by HPLC analysis and the K(m) and Vmax values calculated based on the data obtained by the fluorimetric method.
Bojarska
E
E
Department of Biophysics, University of Warsaw, Poland.
Kraciuk
R
R
Wierzchowski
J
J
Wieczorek
Z
Z
Stepiński
J
J
Jankowska
M
M
Starzyńska
E
E
Guranowski
A
A
Darzynkiewicz
E
E
D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009363:Neoplasm Proteins; D010944:Plants / Q000201:enzymology; D011506:Proteins / Q000378:metabolism*; D012315:RNA Caps; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D011506:Proteins; D012315:RNA Caps; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000099170:Bis(5'-adenosyl)triphosphatase