Culture media and their components differ in their ability to scavenge reactive oxygen species in the plasmid relaxation assay.
OBJECTIVE
To investigate the modulation of DNA-damaging effects of reactive oxygen species by media composition.


METHODS
In vitro study.


METHODS
Academic medical center.


METHODS
None.


METHODS
None.


METHODS
Plasmid relaxation.


RESULTS
Ham's F-10 medium, 1% Percoll, superoxide dismutase (1, 10, or 100 IU), and synthetic serum substitute did not affect DNA damage by reactive oxygen species and did not have any effect on plasmid DNA damage. Plasmid DNA damage was partially inhibited in the presence of P-1 and human tubal fluid media. Human serum albumin, phenol red, glucose, polyvinyl alcohol, polyvinylpyrrolidone, sucrose, and HEPES also were found to protect DNA from damage.


CONCLUSIONS
In vitro fertilization media and their components vary widely in the way they affect DNA damage by reactive oxygen species.
Ermilov
A
A
Department of Obstetrics and Gynecology, Hutzel Hospital/Wayne State University, Detroit, Michigan 48201, USA.
Diamond
M P
MP
Sacco
A G
AG
Dozortsev
D D
DD
D002374:Catalase / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000378:metabolism; D004270:DNA, Circular / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D016166:Free Radical Scavengers / Q000378:metabolism*; D006531:HEPES / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D004270:DNA, Circular; D004278:DNA, Superhelical; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D002374:Catalase; D006531:HEPES