Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus.
The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methicillin-susceptible isolates from cultures of blood from consecutive patients. Also, 267 methicillin-resistant isolates that comprised 248 different phage types were tested. Methicillin resistance was defined as the presence of the mecA gene. Of the 267 mecA gene-positive isolates, 263 were positive by the MRSA screen test (sensitivity, 98.5%), and all the mecA-gene negative strains were negative by the MRSA screen test (specificity, 100%). The oxacillin agar screen test detected methicillin resistance in 250 of the mecA gene-positive isolates (sensitivity, 93.6%). The sensitivity of the MRSA screen test was statistically significantly higher than the sensitivity of the oxacillin agar screen test (P < 0. 05). The MRSA screen test is a highly sensitive and specific test for the detection of methicillin resistance. Also, it offers results within half an hour and is easy to perform, which makes this test a valuable tool in the ongoing battle against methicillin-resistant S. aureus.
van Griethuysen
A
A
Department of Clinical Microbiology, St. Elisabeth Hospital, Tilburg, The Netherlands. a.vgriethuysen@worldonline.nl
Pouw
M
M
van Leeuwen
N
N
Heck
M
M
Willemse
P
P
Buiting
A
A
Kluytmans
J
J
D007841:Latex Fixation Tests; D016106:Methicillin Resistance; D013211:Staphylococcus aureus / Q000302:isolation & purification*