[An experimental study on the effect of nitric oxide synthase in bronchial asthma].
To study the role of nitric oxide synthase (NOS) in rat experimental bronchial asthma. 3H-arginine/3H-L-citrulline conversion technique was used to assay NOS activity of rat lung tissue and histochemical staining method for detect NADPH-d diaphorase. The results revealed that there were significant increase in the level of iNOS activity in asthma group from 152.39% to 249.40%, but the cNOS activity reduced from 64.84% to 61.81% (P < 0.05-0.01). Histochemical staining of NADPH-d showed deep staining of trachial and bronchial epithelium in asthma group. These results suggested that NOS plays a role in regulating airway inflammation and bronchial responsiveness. cNOS possesses a down regulatory effect, while iNOS upregulatory. The occurrence of airway inflammation is earlier than that of smooth muscle contraction and endothelial injury.
Pan
H
H
First Hospital, West China University of Medical Sciences, Chengdu.
Wang
Z
Z
Zhu
Y
Y
D000818:Animals; D001249:Asthma / Q000201:enzymology*; D001980:Bronchi / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D008168:Lung / Q000201:enzymology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D017207:Rats, Sprague-Dawley; D014132:Trachea / Q000201:enzymology
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D009252:NADPH Dehydrogenase