title: [A novel Lp(a) assay not affected by apo(a) size polymorphism].
abstract: Apo(a), the protein moiety of Lp(a), has size polymorphism that derives from the variable number of K4 type 2 repeats. We have developed three ELISA method with using monoclonal antibodies against the K4 type 2 repeats, the K4 type 5-protease domain, and the K5 protease domain of the apo(a) molecule. The commercial assay kits were correlated with the results from ELISA that utilized a monoclonal antibody against the K4 type 2 but indicated poor correlation with an ELISA method using anti Lp(a) K4 type 5-protease domain monoclonal antibody. Serum Lp(a) values were 1.8-fold overestimated by the commercial assay kits in samples with S4 type of the largest isoform, but our ELISA method in which the apo(a) K4 type 5-protease domain is used as labeled antibody and capture antibody was not affected by size polymorphism.
authors.affiliation: Central Institute, Shinotest Corporation, Sagamihara.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizutani
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: A
authors.initials: A
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Kubono
authors.forename: I
authors.initials: I
authors.lastname: Sakurabayashi
mesh_terms: D000911:Antibodies, Monoclonal; D001054:Apolipoproteins A / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D017270:Lipoprotein(a) / Q000032:analysis*; D011110:Polymorphism, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D001054:Apolipoproteins A; D017270:Lipoprotein(a)