Cloning and analysis of the rnc-era-recO operon from Pseudomonas aeruginosa.
The rnc operon from Pseudomonas aeruginosa has been cloned and characterized. The three genes comprising this operon, rnc, era, and recO, are arranged similarly to those in some other gram-negative bacteria. Multicopy plasmids carrying the rnc operon of P. aeruginosa functionally complement mutations of the rnc, era, and recO genes in Escherichia coli. In particular, the P. aeruginosa era homolog rescues the conditional lethality of era mutants in E. coli, and the presumptive protein has 60% identity with the Era of E. coli. We discuss these data and evidence suggesting that a GTPase previously purified from P. aeruginosa and designated Pra is not an Era homolog.
Powell
B
B
Gene Regulation and Chromosome Biology Laboratory, ABL-Basic Research Program, Frederick Cancer Research and Development Center, National Cancer Institute, Frederick, Maryland 21702-1201, USA.
Peters
H K
HK
Nakamura
Y
Y
Court
D
D
D001426:Bacterial Proteins / Q000235:genetics*; D015153:Blotting, Western; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000235:genetics*; D029968:Escherichia coli Proteins; D020558:GTP Phosphohydrolases / Q000032:analysis / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D016601:RNA-Binding Proteins; D043244:Ribonuclease III
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016601:RNA-Binding Proteins; C081006:RecO protein, E coli; C068027:era protein, E coli; D004722:Endoribonucleases; D043244:Ribonuclease III; C468366:ribonuclease III, E coli; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins