title: An inducible human immunodeficiency virus type 1 (HIV-1) vector which effectively suppresses HIV-1 replication.
abstract: Recently, gene therapy vectors based upon the human immunodeficiency virus type 1 (HIV-1) genome have been developed. Here, we create an HIV-1 vector which is defective for all HIV-1 genes, but which maintains cis-acting elements required for efficient packaging, infection, and expression. In T cells transduced by this vector, vector expression is low but efficiently induced following HIV-1 infection. Remarkably, although the HIV-1 vector does not contain specific anti-HIV-1 therapeutic genes, the presence of the vector alone is sufficient to inhibit the spread of HIV-1 infection. The mechanism of inhibition is likely to be at the level of competition for limiting substrates required for either efficient packaging or reverse transcription, thereby selecting against propagation of wild-type HIV-1. These results provide proof of a concept for potential application of a novel HIV-1 vector in HIV-1 disease.
authors.affiliation: Departments of Microbiology & Immunology and Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: An
authors.forename: K
authors.initials: K
authors.lastname: Morizono
authors.forename: Q X
authors.initials: QX
authors.lastname: Li
authors.forename: S H
authors.initials: SH
authors.lastname: Mao
authors.forename: S
authors.initials: S
authors.lastname: Lu
authors.forename: I S
authors.initials: IS
authors.lastname: Chen
mesh_terms: D002461:Cell Line, Transformed; D005822:Genetic Vectors / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D019065:Virus Assembly; D014779:Virus Replication