title: Expression of the MMP-1 in human pancreatic carcinoma: relationship with prognostic factor.
abstract: Matrix metalloproteinases (MMPs) have been implicated in the invasion and metastasis of tumor cells. To elucidate the involvement of MMP-1 in human pancreatic ductal adenocarcinoma, we performed immunohistochemical analysis on tissues from 2 fetal pancreases, 5 normal pancreases, 6 cases of chronic pancreatitis, and 46 pancreatic ductal adenocarcinomas. In addition, among the pancreatic carcinomas, we compared MMP-1 expression in relation to the degree of differentiation, lymph node metastasis, and depth of invasion of the carcinoma. MMP-1 was expressed faintly in fetal and normal pancreatic tissues. Among the 46 pancreatic carcinomas, 33 (72%) showed positive staining for the MMP-1 protein. There was no difference in the degree of differentiation. In situ hybridization confirmed the presence of MMP-1 mRNA in the pancreatic carcinomas. Expression of MMP-1 mRNA was also detected in two human pancreatic carcinoma cell lines and three pancreatic carcinoma tissues by the reverse transcription polymerase chain reaction method. MMP-1 was expressed in the carcinoma cells themselves and in stromal fibroblasts. Patients with MMP-1 positivity in the primary site had a significantly poorer prognosis than patients who were MMP-1 negative (P < .05). MMP-1 expression, however, had no relation to the presence of lymph node metastasis, tumor size, or tumor-node-metastasis stage in pancreatic carcinomas. These findings suggest that MMP-1 expression is related to the carcinogenesis and prognosis of human pancreatic ductal adenocarcinoma.
authors.affiliation: Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: J
authors.initials: J
authors.lastname: Shiozawa
authors.forename: S
authors.initials: S
authors.lastname: Naito
authors.forename: T
authors.initials: T
authors.lastname: Kanematsu
authors.forename: I
authors.initials: I
authors.lastname: Sekine
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D017364:Collagenases / Q000032:analysis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D020781:Matrix Metalloproteinase 1; D008875:Middle Aged; D010179:Pancreas / Q000201:enzymology; D010183:Pancreatic Ducts / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012313:RNA; D017364:Collagenases; D020781:Matrix Metalloproteinase 1