Interferon-gamma gene expression during acute graft-versus-host disease: relationship to MHC induction and tissue injury.
The pathogenetic role of interferon-gamma (IFN-gamma) in acute graft-versus-host disease (GVHD) was examined in a murine model. IFN-gamma gene expression was evaluated by northern blotting and mRNA in situ hybridization. The temporal and tissue specific patterns of IFN-gamma gene expression were related to the patterns of major histocompatibility complex (MHC) antigen induction and of tissue injury. Markedly increased levels of IFN-gamma transcripts were seen in the spleen during the early lymphoproliferative phase and coincided with widespread MHC induction in non-lymphoid tissues. Increased IFN-gamma transcripts were also found in the non-lymphoid target tissues during the phase of subsequent tissue injury. These findings support a role for IFN-gamma in leading to widespread MHC induction during acute GVHD and suggest that IFN-gamma may also contribute to target tissue injury during acute GVHD.
Parfrey
N A
NA
Department of Pathology, University College Dublin and St Vincent's Hospital, Dublin, Ireland.
El-Sheikh
A
A
Monckton
E A
EA
Cockfield
S M
SM
Halloran
P F
PF
Linetsky
E
E
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000276:immunology; D015870:Gene Expression; D006086:Graft vs Host Disease / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000235:genetics*; D008099:Liver / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009206:Myocardium / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014059:Tongue / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma