Nucleotide sequences of the mouse globin beta gene cDNAs in a wild derived new haplotype Hbb(w1).
Haplotypes of the beta-globin gene complex (Hbb) in laboratory mice have been defined as d, p, and s. We previously found a new haplotype w1 in wild mice collected from northwestern China. This study analyzed the nucleotide sequences of b1 and b2-globin gene cDNAs of both the p and w1 haplotypes, in comparison with those of the d haplotype. In Hbb-b1 cDNA, six base substitutions were found between the d and w1 haplotypes and also between p and w1, but none existed between d and p. In Hbb-b2 cDNA, three base substitutions were found between the d and w1 haplotypes and also between d and p, but none between p and w1. This result indicated that the Hbb gene complex of the p haplotype carries b1(d) and b2(w1) genes and is probably a recombinant between d and w1 haplotypes. The hemoglobin containing the W1 phenotype showed oxygen-binding properties identical with those of the hemoglobins containing D and P phenotypes.
Ueda
Y
Y
Laboratory of Animal Cell Technology, Fukuyama University, Fukuyama, Hiroshima-ken, 729-0292, Japan.
Miyashita
N
N
Imai
K
K
Yamaguchi
Y
Y
Takamura
K
K
Notohara
M
M
Shiroishi
T
T
Kawashima
T
T
Ning
L
L
Wang
C
C
Wu
X
X
Moriwaki
K
K
D000818:Animals; D000835:Animals, Wild; D001208:Asia; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D005914:Globins / Q000235:genetics* / Q000378:metabolism; D006239:Haplotypes; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010100:Oxygen / Q000097:blood; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018076:DNA, Complementary; D006454:Hemoglobins; D005914:Globins; D010100:Oxygen