Interleukin-3 or immunoglobulin E promotes expression of protein kinase C delta gene in murine mast cells.
We reported previously that protein kinase C delta (PKCdelta) is the main isoenzyme in various types of murine mast cells. In the present study we investigated the regulation of expression of PKCdelta gene in murine mast cells in vitro and in vivo. The mRNA expressions of PKCdelta were promoted in response to interleukin-3 (IL-3) or immunoglobulin E (IgE) in mouse mastocytoma P-815 cells. In addition we have evaluated the mast cells which express PKCdelta mRNA in IgE-dependent passive cutaneous anaphylaxis reaction, using in situ hybridization with the antisense riboprobe in skin. These results indicate that mast cell activation can induce a marked promotion in steady state levels of PKCdelta mRNA.
Kim
H M
HM
College of Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, South Korea.
Ko
S G
SG
Kim
J J
JJ
Chung
Y T
YT
An
N H
NH
D000818:Animals; D015152:Blotting, Northern; D004140:Dinitrophenols / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology / Q000494:pharmacology*; D017403:In Situ Hybridization; D007377:Interleukin-3 / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D011493:Protein Kinase C / Q000235:genetics*; D051745:Protein Kinase C-delta; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
D004140:Dinitrophenols; D007377:Interleukin-3; D007527:Isoenzymes; D012333:RNA, Messenger; D007073:Immunoglobulin E; C497259:Prkcd protein, mouse; C497260:Prkcd protein, rat; D011493:Protein Kinase C; D051745:Protein Kinase C-delta