title: Lack of crossreaction with Bet v 1 in patients sensitized to Dau c 1, a carrot allergen.
abstract: BACKGROUND
  Pollen-related food allergies to fresh fruit and vegetables are a well-known clinical phenomenon. Allergens related to Bet v 1 are responsible for these cross-reactions.
  
  
  OBJECTIVE
  To characterize the allergen recognized by four carrot-allergic patients.
  
  
  METHODS
  Sera from four patients showing strong immediate systemic reactions after contact or ingestion of raw carrot were studied by immunoblotting. The 18-kD allergen, named Dau c 1, was isolated by ethanol precipitation and specific extraction after SDS-PAGE and its N-terminal amino acid sequence was determined.
  
  
  RESULTS
  All the patients had significant levels of specific IgE to carrot, but no specific IgE to birch pollen was detected in any of them. IgE immunodetection with the sera only recognized a single band of around 18 kD in raw carrot and in celery (with weaker reaction). No reactive band was found with birch pollen. These results were confirmed using a polyclonal anti-carrot antiserum. The carrot IgE-binding protein had a pl of 4.2 and its N-terminal sequence was homologous to that of Bet v 1 and to allergens previously described in celery and other foods. The four patients studied were not sensitized to birch pollen and three of them tolerated fruit ingestion.
  
  
  CONCLUSIONS
  The whole study indicated that a sensitization to Dau c 1 induces IgE antibodies that do not cross-react with birch pollen allergens.
authors.affiliation: Centro de Investigación Clinica, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Moneo
authors.forename: M
authors.initials: M
authors.lastname: Gómez
authors.forename: R
authors.initials: R
authors.lastname: Sánchez-Monge
authors.forename: E
authors.initials: E
authors.lastname: Alday
authors.forename: M
authors.initials: M
authors.lastname: de las Heras
authors.forename: I
authors.initials: I
authors.lastname: Esteban
authors.forename: A
authors.initials: A
authors.lastname: Bootello
authors.forename: G
authors.initials: G
authors.lastname: Salcedo
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant; D003429:Cross Reactions / Q000276:immunology; D018552:Daucus carota / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D010940:Plant Proteins / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; C061312:Bet v 1 allergen, Betula; D012967:Sodium Dodecyl Sulfate