title: Effects of hyperin on free intracellular calcium in dissociated neonatal rat brain cells.
abstract: OBJECTIVE
  To study the effects of hyperin (Hyp) on free intracellular calcium concentration ([Ca2+]i) of brain cells.
  
  
  METHODS
  The neonatal rat brain cells were dissociated. [Ca2+]i in presence and absence of extracellular high K+, L-glutamic acid (Glu), 5-hydroxytryptamine (5-HT), and norepinephrine (NE) were assayed with Fura 2-AM.
  
  
  RESULTS
  The resting [Ca2+]i in Hanks' solution (CaCl2 1.3 mmol.L-1) was (208 +/- 12) nmol.L-1 (n = 17). Hyp had no significant effects on the resting [Ca2+]i. Hyp 1.0, 4.0, and 16.0 mumol.L-1 markedly inhibited the increase of [Ca2+]i evoked by K+ 50 mmol.L-1 in a concentration-dependent manner. Hyp 16.0 mumol.L-1 inhibited the increases of [Ca2+]i induced by NE 1, 2, 4, and 8 mumol.L-1. Hyp (16.0 mumol.L-1) also markedly attenuated 5-HT and Glu-induced increase of [Ca2+]i.
  
  
  CONCLUSIONS
  Hyp possessed inhibitory effects on influx of Ca2+ in the neonatal rat brain cells.
authors.affiliation: Department of Pharmacology, Anhui Medical University, Hefei, China. aydxt@public.ah.hf.cn
authors.forename: Z W
authors.initials: ZW
authors.lastname: Chen
authors.forename: C G
authors.initials: CG
authors.lastname: Ma
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001693:Biological Transport, Active; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002469:Cell Separation; D009638:Norepinephrine / Q000037:antagonists & inhibitors; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012970:Sodium Glutamate / Q000037:antagonists & inhibitors
chemical_list: D002121:Calcium Channel Blockers; C021304:hyperoside; D011794:Quercetin; D002118:Calcium; D012970:Sodium Glutamate; D009638:Norepinephrine