Analysis of cell surface properties using derivatized agarose beads.
An assay has been developed to analyse cell surface properties using agarose beads derivatized with amino acids, sugars, proteins, and other molecules. The assay is simple and rapid and is useful to identify new cell surface markers. Various species and strains of yeast, paramecium, and Euglena were tested for their ability to bind to over 100 types of derivatized beads. A variety of specificity studies were performed in order to understand the nature of cell-bead binding. Our results indicate that cell-bead binding is often specific enough to distinguish between configurational isomers and spacer sizes and can be blocked by addition of specific molecules to the incubation medium. In some cases, different species or strains differed only by their binding to a single bead type. This simple and rapid assay may help to uncover new cell surface receptors and may lead to the development of clinically useful compounds for therapeutic applications.
Salbilla
B A
BA
Department of Biology, California State University, Northridge 91330-8303, USA.
Vaghefi
H
H
Chhabra
P
P
Hall
G
G
Brown
D
D
Sadoughi
F
F
Francisco
E
E
Attas
L
L
Walker
S L
SL
Nguyen
B N
BN
Oppenheimer
S B
SB
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism*; D005056:Euglena gracilis / Q000378:metabolism / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008863:Microspheres; D008968:Molecular Conformation; D010247:Paramecium / Q000378:metabolism / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000378:metabolism*; D013499:Surface Properties
D000596:Amino Acids; D037102:Lectins; D008565:Membrane Proteins; D012685:Sepharose