Functional interaction between PomA and PomB, the Na(+)-driven flagellar motor components of Vibrio alginolyticus.
Four proteins, PomA, PomB, MotX, and MotY, appear to be involved in force generation of the sodium-driven polar flagella of Vibrio alginolyticus. Among these, PomA and PomB seem to be associated and to form a sodium channel. By using antipeptide antibodies against PomA or PomB, we carried out immunoprecipitation to verify whether these proteins form a complex and examined the in vivo stabilities of PomA and PomB. As a result, we could demonstrate that PomA and PomB functionally interact with each other.
Yorimitsu
T
T
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan.
Sato
K
K
Asai
Y
Y
Kawagishi
I
I
Homma
M
M
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D005407:Flagella / Q000737:chemistry / Q000502:physiology*; D009068:Movement; D011485:Protein Binding; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000502:physiology*; D014733:Vibrio / Q000737:chemistry / Q000502:physiology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C103352:PomA protein, bacteria; C103353:PomB protein, bacteria; D015222:Sodium Channels; D012964:Sodium