FUT-175 inhibits the production of IL-6 and IL-8 in human monocytes.
We investigated the effect of FUT-175, a serine protease inhibitor, on the production of pro-inflammatory cytokines, interleukin-6 (IL-6) and interleukin-8 (IL-8), by monocytes stimulated with lipopolysaccharide (LPS). Monocytes isolated from healthy volunteers were incubated with LPS and various concentrations of FUT-175 for 12 hours. The productions of both IL-6 and IL-8, measured by enzyme-linked immunosorbent assay, were significantly inhibited by FUT at the concentration of 1 to 100 microg/ml in a dose-dependent manner. Furthermore, the expressions of IL-6 and IL-8 mRNAs were also inhibited by FUT-175. These data suggest that FUT-175 may reduce the pathological inflammatory conditions associated with enhanced production of proinflammatory cytokines including IL-6 and IL-8.
Sugita
H
H
Department of Surgery II, Kumamoto University Medical School, Japan.
Ishiko
T
T
Ikei
S
S
Hirota
M
M
Ogawa
M
M
D001550:Benzamidines; D004305:Dose-Response Relationship, Drug; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D000093242:Interleukin-6 Inhibitors
D001550:Benzamidines; D006146:Guanidines; D007377:Interleukin-3; D015850:Interleukin-6; D012313:RNA; D015842:Serine Proteinase Inhibitors; D000093242:Interleukin-6 Inhibitors; C032855:nafamostat