Expression of intercellular adhesion molecule-1 in rat inner ear due to bacterial otitis media.
Expressions of anti-Streptococcus pneumoniae antibody and anti-intercellular adhesion molecule-1 (ICAM-1) antibody in the inner ear were studied immunohistochemically in rats following inoculation of S pneumoniae type 6A into the middle ear cavity. Positive staining with anti-S pneumoniae antibody was detected in the marginal cells of the stria vascularis of rats sacrificed 3 days after S pneumoniae inoculation, but almost no staining was detected in those sacrificed at 14 days. Strong ICAM-1 expression was detected in the basal cell layer of the stria vascularis of rats sacrificed 3 days after inoculation, but the intensity of the staining had decreased by 14 days. These results suggest that the stria vascularis may be a site of the inner ear damage that follows bacterial inoculation into the middle ear cavity. The up-regulated expression of ICAM-1 in the basal cell layer may represent a reaction of the inner ear to the bacterial otitis media.
Sone
M
M
Department of Otorhinolaryngology, Nagoya University School of Medicine, Japan.
Russlie
H Q
HQ
Canafax
D M
DM
Paparella
M M
MM
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000276:immunology; D010033:Otitis Media / Q000378:metabolism* / Q000382:microbiology*; D011008:Pneumococcal Infections / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013296:Streptococcus pneumoniae / Q000276:immunology / Q000378:metabolism*
D000907:Antibodies, Bacterial; D018799:Intercellular Adhesion Molecule-1