title: Correlation of survival time with size of axonal swellings in diffuse axonal injury.
abstract: Widespread damage to axons in the white matter of the brain is a well-recognised consequence of non-missile head injury. This diffuse axonal injury is characterised by a gradual swelling of the axon associated with an accumulation of cellular organelles and proteins. We have investigated the relationship between the size of the swellings of the damaged axon with survival time in post-mortem brain tissue. Sixty-six cases of head injury with known length of post-traumatic survival were selected for study, and immunohistochemistry for beta-amyloid precursor protein (betaAPP) was carried out. The minimum diameter of the betaAPP-immunolabelled damaged axons was measured in micrometers using the IBAS image analysis system. There was a strong, positive and significant relationship between the mean size of axonal swelling and survival time which plateaued at around 85 h post injury. With longer survival times the situation becomes more complex. betaAPP immunolabelling of damaged axons can contribute evidence about trauma and post-injury survival time in the forensic setting but should always be assessed with other evidence.
authors.affiliation: The General Infirmary at Leeds, School of Medicine, Division of Clinical Sciences: Pathological Services, University of Leeds, UK.
authors.forename: A E
authors.initials: AE
authors.lastname: Wilkinson
authors.forename: L R
authors.initials: LR
authors.lastname: Bridges
authors.forename: S
authors.initials: S
authors.lastname: Sivaloganathan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D001369:Axons / Q000378:metabolism / Q000473:pathology*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D006259:Craniocerebral Trauma / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D016019:Survival Analysis; D014949:Wounds, Nonpenetrating / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D016564:Amyloid beta-Protein Precursor