Activity of enzymes catalyzing formation of beta-L-fucosyl phosphate and GDP-beta-L-fucose in amphibian tissues and their application in chemo-enzymic synthesis of GDP-beta-L-fucose.
Amphibian oviduct and liver were shown to contain enzymes that catalyze the formation of GDP-beta-L-fucose from GDP-alpha-D-mannose or L-fucose. The conversion of L-fucose into beta-L-fucopyranosyl phosphate was achieved on a preparative scale using high-activity fucokinase in toad liver extracts. For chemo-enzymic preparation of GDP-beta-L-fucose, a convenient modification for pyrophosphate synthesis through phosphomorpholidate which does not require anhydrous conditions is suggested.
Druzhinina
T N
TN
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
Utkina
N S
NS
Chan
K
K
Strecker
G
G
Shibaev
V N
VN
D000818:Animals; D002023:Bufo bufo; D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005643:Fucose / Q000031:analogs & derivatives* / Q000096:biosynthesis; D006154:Guanosine Diphosphate Fucose / Q000096:biosynthesis*; D006600:Hexosephosphates / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D010057:Oviducts / Q000201:enzymology*; D010999:Pleurodeles
D004798:Enzymes; D006600:Hexosephosphates; D006154:Guanosine Diphosphate Fucose; C081214:fucopyranosyl phosphate; D005643:Fucose