title: Tumour cells engineered to secrete interleukin-15 augment anti-tumour immune responses in vivo.
abstract: We examined the effect of interleukin-15 (IL-15) gene transfer into tumour cells on the host's anti-tumour response. In BALB/c mice IL-15 producing Meth-A cells (Meth-A/IL-15) underwent complete rejection, in a response characterized by massive infiltration of CD4+ T-cells and neutrophils. In contrast, Meth-A cells transfected with vector alone (Meth-A/Neo) grew rapidly. Moreover, rechallenged parental cells also were rejected in association with CD8* T-cell infiltration. However, in nude mice there was no drastic difference between Meth-A/IL-15 and Meth-A/Neo cells. These results demonstrate that IL-15-secreting tumour cells can stimulate local and systemic T-cell-dependent immunity and therefore may have a potential role in cancer therapy.
authors.affiliation: Department of Surgery II, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hazama
authors.forename: T
authors.initials: T
authors.lastname: Noma
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Iizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Ogura
authors.forename: K
authors.initials: K
authors.lastname: Yoshimura
authors.forename: E
authors.initials: E
authors.lastname: Inoguchi
authors.forename: M
authors.initials: M
authors.lastname: Hakozaki
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Oka
mesh_terms: D000818:Animals; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D019409:Interleukin-15 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D014162:Transfection
chemical_list: D019409:Interleukin-15