[Development of a serum lipoprotein A-I immunoassay method].
We developed an immunoturbidimetric assay method for lipoprotein A-I by treating sera with an anti-apo lipoprotein A-II and A-I antibody sequentially. The assay is sensitive to detect lipoprotein A-I as little as 155 mg/l with good precision. There was a good correlation in the level of serum lipoprotein A-I between the present assay and a conventional differential electroimmunoassay on ready-to-use plates (r = 0.947; p < 0.001). Reference intervals in normal healthy adults (n = 109) ranged from 284 to 736 mg/l. The values were significantly lower in patients with coronary artery disease than in normal subjects (p < 0.001). The present assay can be useful to analyze patho-physiologic events in various lipid disorders.
Shishino
K
K
Department of Laboratory and Clinical Medicine, Ehime University Medical School.
Hitsumoto
Y
Y
Osawa
H
H
Makino
H
H
Saheki
S
S
D000328:Adult; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D017270:Lipoprotein(a) / Q000031:analogs & derivatives* / Q000097:blood; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry
D017270:Lipoprotein(a); C078260:lipoprotein A-I