Production of a new D-amino acid oxidase from the fungus Fusarium oxysporum.
The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives as inducers. The best inducers were D-alanine (2.7 microkat/g of dry biomass) and D-3-aminobutyric acid (2.6 microkat/g of dry biomass). The addition of zinc ions was necessary to permit the induction of peroxisomal D-amino acid oxidase. Bioreactor cultivations were performed on a 50-liter scale, yielding a volumetric D-amino acid oxidase activity of 17 microkat liter(-1) with D-alanine as an inducer. Under oxygen limitation, the volumetric activity was increased threefold to 54 microkat liter(-1) (3,240 U liter(-1)).
Gabler
M
M
Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, 38106 Braunschweig, Germany.
Fischer
L
L
D019149:Bioreactors; D001709:Biotechnology; D003605:D-Amino-Acid Oxidase / Q000096:biosynthesis*; D005670:Fusarium / Q000201:enzymology*; D010100:Oxygen
D003605:D-Amino-Acid Oxidase; D010100:Oxygen