Mouse dendritic cells express the P2X7 purinergic receptor: characterization and possible participation in antigen presentation.
Immune cells express P2 purinoceptors of the P2Y and P2X subtypes. In the present work, we show that three dendritic cell (DC) lines, D2SC/1, CB1, and FSDC, representative of immature DCs, express the P2X7 (formerly P2Z) receptor, as judged from RT-PCR amplification, reactivity to a specific antiserum, and pharmacological and functional evidence. Receptor expression is higher in FSDC cells, a cell line that is functionally more mature than D2SC/1 and CB1. From the wild-type DC population, we selected cell clones lacking the P2X7R (P2X7less). We also used a P2XR blocker, oxidized ATP, to irreversibly inhibit the P2X7R. Ability of P2X7less FSDCs or of oxidized ATP-inhibited FSDCs to stimulate Ag-specific TH lymphocytes was severely decreased although Ag endocytosis was minimally affected. During coculture with TH lymphocytes, wild-type FSDC secreted large amounts of IL-1beta. Release of this cytokine was reduced in P2X7less DCs. These data show that DCs express the P2X7 purinoceptor and suggest a correlation between P2X7R expression and Ag-presenting activity.
Mutini
C
C
Section of General Pathology, Department of Experimental and Diagnostic Medicine, Biotechnology Center, University of Ferrara, Italy.
Falzoni
S
S
Ferrari
D
D
Chiozzi
P
P
Morelli
A
A
Baricordi
O R
OR
Collo
G
G
Ricciardi-Castagnoli
P
P
Di Virgilio
F
F
D000255:Adenosine Triphosphate / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000276:immunology; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005333:Fetus; D007106:Immune Sera / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis* / Q000737:chemistry* / Q000276:immunology; D058486:Receptors, Purinergic P2X7; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology
D007106:Immune Sera; C546469:P2rx7 protein, mouse; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate; D002118:Calcium