title: Reversal of synaptic vesicle docking at central synapses.
abstract: We used quantitative fluorescence imaging of vesicles labeled with membrane-soluble dyes to determine rates of undocking and spontaneous exocytosis of vesicles docked to the active zone of hippocampal synapses in culture. Individual vesicles undock about once per two minutes and spontaneously exocytose about once per eight minutes. Thus, not only does undocking occur, but it is over threefold faster than spontaneous fusion.
authors.affiliation: Howard Hughes Medical Institute, and Molecular Neurobiology Lab, Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: V N
authors.initials: VN
authors.lastname: Murthy
authors.forename: C F
authors.initials: CF
authors.lastname: Stevens
mesh_terms: D002478:Cells, Cultured; D004558:Electric Stimulation; D005089:Exocytosis / Q000502:physiology; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D013569:Synapses / Q000502:physiology*; D013572:Synaptic Vesicles / Q000502:physiology*; D013997:Time Factors
chemical_list: C073804:FM1 43; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds