title: Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP.
abstract: The repetitive sequence of Leishmania major gene B protein (GBP) has previously been shown to be a useful tool in the diagnosis of cutaneous leishmaniasis (CL). Here, we have assessed enzyme-linked immunosorbent assays (ELISAs) using recombinant L. donovani GBP (rGBP) and a peptide sequence of L. donovani GBP (GBPP) in the diagnosis of L. donovani infections in Sudan. The sensitivity of the rGBP ELISA in diagnosing visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) was 92% and 93%, respectively. In contrast, the sensitivity of the GBPP ELISA was 55% for VL and 63% for PKDL. Plasma antibody reactivity of donors with VL and PKDL remained high for an extended period after the end of treatment. Antibody-reactivity to rGBP and GBPP was detected in 71% and 14% of plasma samples from CL patients, respectively. Plasma from healthy Sudanese donors living in an area endemic for malaria but free of leishmaniasis was negative in both assays.
authors.affiliation: Centre for Medical Parasitology at Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark. parasite@biobase.dk
authors.forename: A T
authors.initials: AT
authors.lastname: Jensen
authors.forename: S
authors.initials: S
authors.lastname: Gasim
authors.forename: T
authors.initials: T
authors.lastname: Moller
authors.forename: A
authors.initials: A
authors.lastname: Ismail
authors.forename: A
authors.initials: A
authors.lastname: Gaafar
authors.forename: M
authors.initials: M
authors.lastname: Kemp
authors.forename: A M
authors.initials: AM
authors.lastname: el Hassan
authors.forename: A
authors.initials: A
authors.lastname: Kharazmi
authors.forename: T M
authors.initials: TM
authors.lastname: Alce
authors.forename: D F
authors.initials: DF
authors.lastname: Smith
authors.forename: T G
authors.initials: TG
authors.lastname: Theander
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007893:Leishmania donovani / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015800:Protozoan Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D020449:Repetitive Sequences, Amino Acid; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins