Enantioseparation of tropa alkaloids by means of anionic cyclodextrin-modified capillary electrophoresis.
Anionic cyclodextrins (CDs), i.e., sulfated CD, sulfobutylether beta-CD (SBE-beta-CD) and heptakis(2,3-di-O-acetyl-6-sulfato)-beta-CD (HDAS-beta-CD), were used to separate the isomers of atropine, homatropine, ipratropium, scopolamine and butylscopolamine. Variations in the pH value and the concentration of the various CDs revealed the sulfated beta-CD to be superior to the other derivatives. In a basic medium at low concentrations of sulfated CD the enantiomers of atropine, homatropine and ipratropium were well resolved in a short migration time, which is appropriate for the routine analysis of the enantiomeric excess. Scopolamine and butylscopolamine compounds could not be separated with either CD.
Wedig
M
M
Pharmazeutisches Institut der Universität Bonn, Germany.
Holzgrabe
U
U
D000838:Anions / Q000737:chemistry*; D001285:Atropine / Q000302:isolation & purification; D001533:Belladonna Alkaloids / Q000302:isolation & purification*; D003505:Cyclodextrins / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009241:Ipratropium / Q000302:isolation & purification; D013997:Time Factors; D014326:Tropanes / Q000302:isolation & purification; D047392:beta-Cyclodextrins
D000838:Anions; D001533:Belladonna Alkaloids; D003505:Cyclodextrins; D014326:Tropanes; D047392:beta-Cyclodextrins; D001285:Atropine; C100236:homatropine; D009241:Ipratropium; C031215:betadex