Cytokine response to infection with the microsporidian, Encephalitozoon cuniculi.
The production of three cytokines, interferon gamma (IFN-gamma), interleukin 10 (IL-10) and interleukin 12 (IL-12), was measured after intraperitoneal infection of immunocompetent Balb/c mice and immunodeficient SCID mice with the microsporidian, Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923. High levels of IFN-gamma were detected in ex vivo cultures of peritoneal exudate cells (PEC) of Balb/c mice, a lower, but earlier IFN-gamma response was observed in PEC from SCID mice. The early IL-10 response was detected in ex vivo cultures of splenocytes from Balb/c but not from SCID mice, explaining a delay in the IFN-gamma response in Balb/c mice. IL-12 was detected in PEC cultures from SCID mice, indicating an alternative pathway of IFN-gamma production by NK cells stimulated by IL-12 derived from macrophages.
Braunfuchsová
P
P
Faculty of Biological Sciences, University of South Bohemia, Ceské Budĕjovice, Czech Republic.
Kopecký
J
J
Ditrich
O
O
Koudela
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D009670:Encephalitozoon cuniculi / Q000276:immunology* / Q000472:pathogenicity; D016890:Encephalitozoonosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D016207:Cytokines; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma