Validation of 14C-urea breath test for diagnosis of Helicobacter pylori.
The aim of this study was to validate the 14C-urea breath test for use in diagnosis of Helicobacter pylori infection. Thirty H. pylori positive patients, based on histologic test and thirty H. pylori negative patients by histology and anti-H. pylori IgG entered the study. Fasting patients drank 5 uCi of 14C-urea in 20 ml of water. Breath samples were collected at 0, 5, 10, 15, 20 and 30 min. The difference of cpm values between the two groups was significant at all the time intervals, besides time 0 (p < 0.0001). At 20 min, the test gave 100% sensitivity and specificity with a cut-off value of 562 cpm. Females were higher expirers than males (p = 0.005). 14C-urea breath test is highly accurate for Helicobacter pylori diagnosis. It is fast, simple and should be the non-invasive test used after treating Helicobacter pylori infection.
Mattar
R
R
Department of Gastroenterology, Hospital of Clinics, University of São Paulo School of Medicine, Brazil. shiroineko@uol.com.br
Silva
F M
FM
Alexandrino
A M
AM
Laudanna
A A
AA
D001706:Biopsy; D001944:Breath Tests / Q000379:methods*; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012737:Sex Factors; D013048:Specimen Handling; D013997:Time Factors; D014508:Urea
D014508:Urea