Localisation of P2X receptors in human salivary gland epithelial cells and human embryonic kidney cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis/Western blotting and immunofluorescence.
Human salivary gland epithelial cells, a continuous cell line derived from an irradiated human salivary gland and human embryonic kidney cell line human embryonic kidney (HEK)293 were examined for the purpose of establishing whether they expressed endogenous P2X ionotropic receptors at any stage in their cycles. HSG cells were found to express P2X1-6 subtypes using both Western blotting and immunofluorescence labeling. HEK293 cells had no detectable levels of P2X1-3 and P2X6 under normal circumstances along with very low levels of P2X4 and P2X5 but when the cells were grown past confluence then all subtypes were expressed on the surface membrane with the exception of P2X2. The results are discussed in terms of the likely influence of ATP acting as an intercellular signaling molecule.
Worthington
R A
RA
Institute for Biomedical Research, Department of Anatomy and Histology, The University of Sydney, NSW, Australia.
Dutton
J L
JL
Poronnik
P
P
Bennett
M R
MR
Barden
J A
JA
D000918:Antibody Specificity; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007668:Kidney / Q000737:chemistry* / Q000196:embryology*; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D012469:Salivary Glands / Q000737:chemistry*
D018048:Receptors, Purinergic P2