[Dynamic studies of Langerhans cell histiocytosis cells. Their contribution to the current concept of the disease. Report of a series of 38 cases].
In vitro explantation of 38 fragments of eosinophilic granuloma of bones was attempted. A satisfactory growth was obtained in nearly 90% of cases. This short-term culture maintained the ultrastructural characteristics and, to a lesser extent, the cytochemical features of the Langerhans cells, confirming the Langerhans cell origin of this cell proliferation. In addition, this procedure was able to demonstrate an immunodependent erythrophagocytosis (3/3) and a preferential fixation of labelled precursors (Glycerol, choline of lipid metabolism as well as labelled dopamine (2/2). All attempts to obtain a permanent cell line and graft to nude mice (even irradiated) failed. Under the in vitro conditions, the Langerhans cells do not divide up but can be readily identified up to 2 or 3 weeks. The contrast between the evident in vivo proliferation and the in vitro quiescent state suggests that some undetermined growth-factors targeted to the Langerhans cell system are missing in our commonly used culture media. The in vitro culture procedure could be of some help to their identification.
Nezelof
C
C
Basset
F
F
Rousseau-Merck
M F
MF
Barbey
S
S
D000818:Animals; D001847:Bone Diseases / Q000473:pathology*; D002460:Cell Line; D004803:Eosinophilic Granuloma / Q000473:pathology*; D006646:Histiocytosis, Langerhans-Cell / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude
Etudes dynamiques des cellules de l'histiocytose Langerhansienne. Leur apport dans le concept actuel de la maladie. A propos d'une série de 38 observations.