title: Human papillomavirus is a necessary cause of invasive cervical cancer worldwide.
abstract: A recent report that 93 per cent of invasive cervical cancers worldwide contain human papillomavirus (HPV) may be an underestimate, due to sample inadequacy or integration events affecting the HPV L1 gene, which is the target of the polymerase chain reaction (PCR)-based test which was used. The formerly HPV-negative cases from this study have therefore been reanalyzed for HPV serum antibodies and HPV DNA. Serology for HPV 16 VLPs, E6, and E7 antibodies was performed on 49 of the 66 cases which were HPV-negative and a sample of 48 of the 866 cases which were HPV-positive in the original study. Moreover, 55 of the 66 formerly HPV-negative biopsies were also reanalyzed by a sandwich procedure in which the outer sections in a series of sections are used for histological review, while the inner sections are assayed by three different HPV PCR assays targeting different open reading frames (ORFs). No significant difference was found in serology for HPV 16 proteins between the cases that were originally HPV PCR-negative and -positive. Type-specific E7 PCR for 14 high-risk HPV types detected HPV DNA in 38 (69 per cent) of the 55 originally HPV-negative and amplifiable specimens. The HPV types detected were 16, 18, 31, 33, 39, 45, 52, and 58. Two (4 per cent) additional cases were only HPV DNA-positive by E1 and/or L1 consensus PCR. Histological analysis of the 55 specimens revealed that 21 were qualitatively inadequate. Only two of the 34 adequate samples were HPV-negative on all PCR tests, as against 13 of the 21 that were inadequate ( p< 0.001). Combining the data from this and the previous study and excluding inadequate specimens, the worldwide HPV prevalence in cervical carcinomas is 99.7 per cent. The presence of HPV in virtually all cervical cancers implies the highest worldwide attributable fraction so far reported for a specific cause of any major human cancer. The extreme rarity of HPV-negative cancers reinforces the rationale for HPV testing in addition to, or even instead of, cervical cytology in routine cervical screening.
authors.affiliation: Department of Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands. JMM.Walboomers@azvu.nl
authors.forename: J M
authors.initials: JM
authors.lastname: Walboomers
authors.forename: M V
authors.initials: MV
authors.lastname: Jacobs
authors.forename: M M
authors.initials: MM
authors.lastname: Manos
authors.forename: F X
authors.initials: FX
authors.lastname: Bosch
authors.forename: J A
authors.initials: JA
authors.lastname: Kummer
authors.forename: K V
authors.initials: KV
authors.lastname: Shah
authors.forename: P J
authors.initials: PJ
authors.lastname: Snijders
authors.forename: J
authors.initials: J
authors.lastname: Peto
authors.forename: C J
authors.initials: CJ
authors.lastname: Meijer
authors.forename: N
authors.initials: N
authors.lastname: Muñoz
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D002584:Cervix Uteri / Q000473:pathology; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D014943:Global Health; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D050725:Papillomavirus E7 Proteins; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012097:Repressor Proteins; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16