A time course study on STR profiles derived from human bone, muscle, and bone marrow.
The use of the polymerase chain reaction (PCR) to define deoxyribonucleic acid (DNA) types at several loci was investigated. PCR was used to amplify nine short tandem repeat (STR) loci along with the amelogenin locus on the X and Y chromosomes using the AmpF/STR Profiler Plus PCR amplification kit (Perkin Elmer). Rib bones were collected from 12 individuals. Five cm portions were buried at a depth of approximately 30 cm and 5 cm portions were left on the surface of the ground. Samples were exposed to the environment for periods of time ranging from two weeks to 17 months. Dried blood standards were prepared for use as reference standards for each rib sample. Bone, muscle, and bone marrow were collected from each sample. DNA from each tissue type was extracted. Complete profile results were obtained from the surface bone samples out to an exposure time of 17 months. None of the muscle or bone marrow samples produced complete profile results beyond eight weeks. All DNA typing results from complete or incomplete profiles were consistent with DNA typing results of the corresponding blood standard. Results suggest that using the AmpF/STR Profiler Plus PCR Amplification Kit is a valid way to establish the DNA profile of tissue types from human remains.
Frank
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WE
Illinois State Police, Forensic Sciences Command, Research and Development Laboratory, Springfield, IL 62702, USA.
Llewellyn
B E
BE
D000483:Alleles; D053523:Amelogenin; D001853:Bone Marrow / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D003746:Dental Enamel Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D018482:Muscle, Skeletal / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012272:Ribs / Q000737:chemistry*; D012732:Sex Determination Analysis; D013997:Time Factors
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA