Confirmed identities of proteins from a two-dimensional map of Syrian hamster embryo cells.
The Syrian hamster embryo (SHE) cell transformation assay is widely used to screen chemicals for carcinogenic potential. However, the biochemical mechanisms of transformation in SHE cells are incompletely understood relative to other rodent systems. Thus identification of proteins which change during transformation can provide clues to biochemical mechanisms. Previously, we published a map of SHE cell proteins based on comparisons to other maps. In this report we provide direct sequence analysis of numerous proteins which were previously identified solely by electrophoretic mobility. Protein sequencing verified original spot identifications and extended the range of identified proteins. The updated map will assist in evaluating biochemical mechanisms of morphological transformation in hamster cells.
Asquith
T N
TN
Miami Valley Laboratories, The Procter & Gamble Company, Cincinnati, OH 45253-8707, USA. asquithtn@pg.com
Gauggel
D L
DL
Esquer-Blasco
R
R
Anderson
N L
NL
Isfort
R J
RJ
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004622:Embryo, Mammalian / Q000737:chemistry*; D008647:Mesocricetus; D051379:Mice; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D017421:Sequence Analysis / Q000379:methods*
D011506:Proteins