A rapid method for detecting and distinguishing clinically important yeasts by heteroduplex mobility assays (HMAs).
Heteroduplex Mobility Assays (HMAs) of 700 bp amplified products of a 17S rDNA region were used to identify and differentiate seven yeast species of clinical importance Candida albicans, Torulopsis (Candida) glabrata, Candida tropicalis, Candida parapsilosis, Candida (Pichia) guilliermondii and Hansenula (Pichia) anomala. Distance of heteroduplex migration (dHE) was found to be negatively correlated to the number of nucleotide differences between amplified DNA sequences.
Olicio
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Genetics Section, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
Almeida
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CA
Seuánez
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D001483:Base Sequence; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D010843:Pichia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D009692:Nucleic Acid Heteroduplexes