The isolation and mapping of 19 tetranucleotide microsatellite markers in the chicken.
Microsatellites consisting of tetranucleotide repeats are more easily, and consequently efficiently, scored than loci consisting of dinucleotides. However, they are much less frequent in the genome. A hybridisation selection protocol was therefore employed to generate a chicken genomic library enriched for inserts containing the tetranucleotide repeat motif (TTTC)n. Forty-five new microsatellite sequences were isolated that mainly consisted of perfect repeats of the tetranucleotide (TTTC) motif. Nineteen markers were mapped in one or both of the East Lansing and Compton international chicken reference populations.
McConnell
S K
SK
Department of Biology, University of Leicester, UK.
Dawson
D A
DA
Wardle
A
A
Burke
T
T
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D015698:Genomic Library; D018895:Microsatellite Repeats
D017931:DNA Primers