[Study on in situ expression of type I and III procollagen mRNAs in different parts of keloid and normal skin].
OBJECTIVE
Based on the histomorphologic differences of three parts of keloid, i.e., invasive, proliferative and senile regions, the study was carried out to explore the differences of expression of type I and type III procollagen mRNAs in the three different parts of keloid and normal skin.


METHODS
Four samples of keloid from four patients and two samples of normal skin were collected. The expression of pro alpha 1 (I) and pro alpha 1 (III) procollagen mRNAs in keloid and normal skin was investigated by in situ hybridization techniques.


RESULTS
Both type I and III procollagen mRNAs levels increased in keloid, especially type I, resulting in elevated ratios of type I to III procollagen mRNA. In keloid, expressive intention of type I and type III procollagen mRNAs was higher in the papillary layer than in the reticular layer. Also the expressive intention of type I and type III in keloid was higher in invasive and proliferative regions than in the senile region. However, expressive intention of type I and type III procollagen mRNAs in the invasive region of keloid was similar to that in the proliferative region.


CONCLUSIONS
The differences of expression of type I and type III procollagen mRNAs in different pathological regions of keloid and normal skin is one cause of forming different pathological regions in keloid.
Bao
W
W
Research Center of Plastic Surgery, Third Clinical School of Beijing Medical University.
Xu
S
S
Guan
B
B
D000328:Adult; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D008297:Male; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D012867:Skin / Q000378:metabolism*
D011347:Procollagen; D012333:RNA, Messenger