Effect of melatonin on production of hydroxyl radical and lactate dehydrogenase during hypoxia in rat cortical slices.
OBJECTIVE
To study the effect of melatonin on the production of hydroxyl radical (.OH) and lactate dehydrogenase (LDH) following hypoxia in cortical slice.


METHODS
Cortical slice was incubated with artificial cerebrospinal fluid (ACSF) in tube. Hypoxia was achieved by substituting 91.6% N2 and 8.4% O2. The salicylate trapping method was used to measure hydroxyl radicals generated. The content of LDH in medium after hypoxia was measured by International Federation of Clinic Chemistry (IFCC) method.


RESULTS
The contents of dihydroxybenzoic acid (DHBA) were increased significantly during hypoxia and reoxygenation in cortical slice. The production of DHBA in reoxygenation was decreased concentration-dependently by melatonin, but not during hypoxia 30 min. The release of LDH during hypoxia was steadily elevated and melatonin decreased the content of LDH after hypoxia.


CONCLUSIONS
Melatonin decreased the injury and production of .OH after hypoxia.
Li
X J
XJ
Department of Neurobiology, Shanghai Medical University, China.
Gu
J
J
Pan
B S
BS
Sun
F Y
FY
D000818:Animals; D015687:Cell Hypoxia; D002540:Cerebral Cortex / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D007770:L-Lactate Dehydrogenase; D008550:Melatonin