Metabolite profiling study of propranolol in rat using LC/MS/MS analysis.
Metabolite profiling is one of the most challenging fields in applied mass spectrometry. Mass spectrometry was used to characterize the metabolites of propranolol, a beta-adrenergic receptor antagonist containing numerous oxidation sites. Propranolol is extensively metabolized, with most metabolites appearing in urine. Urine samples were collected from young adult male Sprague-Dawley rats. Structural identification of various metabolites was performed by LC/MS/MS, using a PE SCIEX triple quadrupole instrument (PE SCIEX API 3000). Metabolites were itemized using several LC/MS/MS techniques, including Q3 full scan and precursor and constant neutral loss experiments. A looped experiment technique revealed the presence of mono- and di-hydroxylated metabolites as well as regio isomers of hydroxy- and dihydroxy-propranolol glucuronides and propranolol glucuronic acid. Propranolol glucuronide was not observed, while the presence of dealkylated metabolites was suggested but not confirmed.
Beaudry
F
F
Advanced Analytical Research and Development Department, Phoenix International Life Sciences Inc., 2350 Cohen Street, St-Laurent, PQ, Canada H4R 2N6.
Yves Le Blanc
J C
JC
Coutu
M
M
Ramier
I
I
Moreau
J P
JP
Brown
N K
NK
D000319:Adrenergic beta-Antagonists / Q000032:analysis / Q000493:pharmacokinetics*; D000465:Algorithms; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D008297:Male; D013058:Mass Spectrometry; D011433:Propranolol / Q000032:analysis / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000319:Adrenergic beta-Antagonists; D007202:Indicators and Reagents; D011433:Propranolol