title: Ca(2+)-activated nonselective cationic current (I(CAN)) in turtle motoneurons.
abstract: The presence of a calcium-activated nonspecific cationic (CAN) current in turtle motoneurons and its involvement in plateau potentials, bistability, and wind-up was investigated by intracellular recordings in a spinal cord slice preparation. In the presence of tetraethylammonium (TEA) and tetrodotoxin (TTX), calcium action potentials evoked by depolarizing current pulses were always followed by an afterdepolarization associated with a decrease in input resistance. The presence of the afterdepolarization depended on the calcium spike and not on membrane potential. Replacement of extracellular sodium by choline or N-methyl-D-glucamine (NMDG) reduced the afterdepolarization, confirming that it was mediated by a CAN current. Plateau potentials and wind-up were evoked in response to intracellular current pulses in the presence of agonist for different metabotropic receptors. Replacement of extracellular sodium by choline or NMDG did not abolish the generation of plateau potentials, bistability, or wind-up, showing that Na(+) was not the principal charge carrier. It is concluded that plateau potentials, bistability and wind-up in turtle motoneurons do not depend on a CAN current even though its presence can be detected.
authors.affiliation: Department of Medical Physiology, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
authors.forename: J F
authors.initials: JF
authors.lastname: Perrier
authors.forename: J
authors.initials: J
authors.lastname: Hounsgaard
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects*; D066298:In Vitro Techniques; D009046:Motor Neurons / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*; D019789:Tetraethylammonium / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014426:Turtles / Q000502:physiology*
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D013779:Tetrodotoxin; D019789:Tetraethylammonium; D009543:Nifedipine