title: The rox1 and rox2 RNAs are essential components of the compensasome, which mediates dosage compensation in Drosophila.
abstract: The rox1 and rox2 RNAs have been suggested to be components of the dosage compensation machinery in Drosophila. We show that both rox RNAs colocalize with the male-specific lethal proteins at hundreds of specific bands along the male X chromosome. The rox RNAs and MSL proteins also colocalize with the X chromosome in all somatic cells and are expressed in the same temporal pattern throughout development. Genetic analysis shows that the functions of the rox genes are redundant and required for the association of the MSL proteins with the male X chromosome. These data provide compelling evidence for a direct function of the rox RNAs in dosage compensation.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305, USA.
authors.forename: A
authors.initials: A
authors.lastname: Franke
authors.forename: B S
authors.initials: BS
authors.lastname: Baker
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D014157:Transcription Factors / Q000378:metabolism; D014960:X Chromosome / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; C092595:Pabp2 protein, Drosophila; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C083348:ROX1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C082132:msl-1 protein, Drosophila; C094166:msl-2 protein, Drosophila; C093904:msl-3 protein, Drosophila