title: Determination of the molecular size distribution of Haemophilus influenzae type b-tetanus toxoid conjugate vaccines by size-exclusion chromatography.
abstract: The molecular size of Haemophilus influenzae (Hib) type b polysaccharide-protein conjugate vaccines is an important physico-chemical parameter which correlates with immunogenicity. This paper describes the experimental conditions for high-performance size-exclusion chromatography on a PL Aquagel-OH 60 column to determine the size distribution of Hib high-molecular-weight conjugate vaccines.
authors.affiliation: Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Parisi
authors.forename: C
authors.initials: C
authors.lastname: von Hunolstein
mesh_terms: D001428:Bacterial Vaccines / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D006193:Haemophilus influenzae / Q000276:immunology*; D008970:Molecular Weight; D013745:Tetanus Toxoid / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry*
chemical_list: D001428:Bacterial Vaccines; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic