title: An inverse relationship between T cell receptor affinity and antigen dose during CD4(+) T cell responses in vivo and in vitro.
abstract: Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.
authors.affiliation: Howard Hughes Medical Institute, Division of Basic Immunology, National Jewish Medical and Research Center, Denver, CO 80206, USA.
authors.forename: W
authors.initials: W
authors.lastname: Rees
authors.forename: J
authors.initials: J
authors.lastname: Bender
authors.forename: T K
authors.initials: TK
authors.lastname: Teague
authors.forename: R M
authors.initials: RM
authors.lastname: Kedl
authors.forename: F
authors.initials: F
authors.lastname: Crawford
authors.forename: P
authors.initials: P
authors.lastname: Marrack
authors.forename: J
authors.initials: J
authors.lastname: Kappler
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D018960:Hyaluronan Receptors / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007117:Immunization, Secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*
chemical_list: D000949:Histocompatibility Antigens Class II; D018960:Hyaluronan Receptors; D011948:Receptors, Antigen, T-Cell