Oxidative stress in the thalamus of Wistar rats treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Experimental parkinsonism was induced in adult Wistar rats by selective nigrostriatal neurotoxine, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in a single dose of 0.09 g/kg, by unilateral intrastriatal application using stereotaxic instrument. Control group included rats treated with 0.9% saline solution in the same manner. Animals were sacrificed by decapitation seven days after the treatment. Total glutathione was measured in the crude mitochondrial fraction of thalamus and striatum. Total glutathione content, as a measure of reduced cell atmosphere, was mutually decreased in the thalamus and striatum of MPTP-treated animals, compared to controls: thalamus ipsi- = 24.8 +/- 3.11, contralateral = 26.81 +/- 5.31; striatum ipsi- = 19.96 +/- 4.13, contralateral = 17.3 +/- 4.09 nmol/mg prot. Mutually depleted glutathione content in the thalamus and contralateral striatum, the structures distant from ipsilateral treated striatum, could indicate on spatial propagation of oxidative stress, not only in the selective vulnerable dopaminergic nigrostriatal neurons, but in the structures included in the motor and cognitive loops of basal ganglia.
Jovanović
M D
MD
Military Medical Academy, Institute for Medical Research, Belgrade.
Ninković
M
M
Malicević
Z
Z
Mihajlović
R
R
Dukić
M
M
Vasiljević
I
I
Jelenković
A
A
Jovicić
A
A
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D018384:Oxidative Stress; D010302:Parkinson Disease, Secondary / Q000209:etiology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013788:Thalamus / Q000378:metabolism*
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D005978:Glutathione