[Changes in the transmembrane potential of thymocytes during dexamethasone- and Ca2(+)-induced apoptosis].
By means of the method of potential-sensitive fluorescent probes is established that under dexametazone-induced apoptosis (0.1-10 microM) the reduction of transmembrane potentials (TMP, delta phi) on plasmatic (delta phi p) and mitochondrial (delta phi m) membranes of hormone-sensitive thymocytes is observed. Dexametazone does not changes transmembrane potentials of glucocorticoid-resistant thymocytes. Ca-ionophore A23187 realistically reduces (at the average on 26%) value of plasmatic TMP both in glucocorticoid-sensitive and glucocorticoid-resistant thymocytes. Change of delta phi is one of the mechanisms to realization an apoptotic process in rats' thymocytes. Dynamics and nature of TMP changes depend on the agent-inductor of apoptosis.
Dukhanin
A S
AS
Department of Molecular Pharmacology and Radiobiology, Russian State Medical University, Moscow, Russia.
Patrashev
D V
DV
Ogurtsov
S I
SI
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D005938:Glucocorticoids / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D051381:Rats; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D005456:Fluorescent Dyes; D005938:Glucocorticoids; D003907:Dexamethasone; D002118:Calcium
Izmenenie transmembrannogo potentsiala timotsitov pri deksametazon- i Ca2(+)-indutsirovannom apoptoze.