title: Studies on the determination of chlorotestosterone and its metabolites in bovine urine.
abstract: Chlorotestosterone and its metabolites were determined in urine samples from bovine animals treated with chlorotestosterone acetate by oral and intramuscular routes. Sample preparation, involving enzymatic deconjugation and solid-phase extraction, was optimised. The effect of different enzyme preparations, pH, and time of incubation were studied. An extraction/clean-up procedure based on solid-phase extraction (C18 cartridge) and liquid-liquid clean-up was developed. Determination of chlorotestosterone and its metabolites was by enzyme immunoassay and GC-MS. Metabolites were converted into their TMS-enol-TMS-ether and TMS-oxime-TMS-ether forms before GC-MS (EI) analysis.
authors.affiliation: Teagasc, National Food Centre, Castleknock, Dublin, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Walshe
authors.forename: M
authors.initials: M
authors.lastname: O'Keeffe
authors.forename: B
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D000284:Administration, Oral; D045930:Anabolic Agents / Q000378:metabolism / Q000652:urine*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007124:Immunoenzyme Techniques; D007273:Injections, Intramuscular; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine*
chemical_list: D045930:Anabolic Agents; D013739:Testosterone; C076775:clostebol