title: Mutations in the voltage-dependent anion channel of the mitochondrial outer membrane cause a dominant nonlethal growth impairment.
abstract: Point mutations at K234 and K236 in the yeast voltage-dependent anion channel 1 (VDAC1) of the mitochondrial outer membrane have been shown to markedly impair the membrane insertion of this protein (Smith et al., 1995; Angeles et al., 1998). Mutants of this type were expressed in vivo in a strain of yeast with a disruption in the VDAC1 gene. Expression of the various VDAC1 forms was under the control of a Gall promoter. Wild-type VDAC1 expression fully complemented the slow growth phenotype caused by the disruption. VDAC1 mutants in which K234 and K236 were replaced by arginine, glutamate, or glutamine caused a more severe negative effect on growth. This effect appeared to be dominant since the mutant VDAC1 forms suppressed growth in a yeast strain that retained its VDAC1 gene. This apparent dominant negative effect on growth did not seem to be specific for any stage of the cell cycle. However, the growth defect was not lethal as the affected cells still could accumulate the vital stain, FUN1. Expression of a mutant in which K234 had been replaced by glutamate had more serious negative growth effects than did a similar mutation at K236. Expression of delta71-116 VDAC1 complemented the VDAC1 disruption; however, expression of the same deletion mutant in which the lysines corresponding to K234 and K236 were mutated to glutamate severely impaired growth. These results have shown that a deficiency of lysine at positions 234 and 236 in VDAC I causes a nonlethal growth defect that is more severe than deletion of 45 amino acids from VDACI or disruption of the VDAC1 gene. They also indicate that there is a hierarchy in the importance of these lysines with mutations at K234 being the more serious.
authors.affiliation: School of Medicine, Wayne State School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: R
authors.initials: R
authors.lastname: Angeles
authors.forename: J
authors.initials: J
authors.lastname: Devine
authors.forename: R
authors.initials: R
authors.lastname: Barret
authors.forename: D
authors.initials: D
authors.lastname: Goebel
authors.forename: E
authors.initials: E
authors.lastname: Blachyl-Dyson
authors.forename: M
authors.initials: M
authors.lastname: Forte
authors.forename: R
authors.initials: R
authors.lastname: McCauley
mesh_terms: D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D007425:Intracellular Membranes / Q000502:physiology*; D007700:Kinetics; D008239:Lysine; D008854:Microscopy, Electron; D008928:Mitochondria / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D018272:Porins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D050995:Voltage-Dependent Anion Channel 1; D050994:Voltage-Dependent Anion Channels
chemical_list: D018272:Porins; D011994:Recombinant Proteins; D050994:Voltage-Dependent Anion Channels; D050995:Voltage-Dependent Anion Channel 1; D008239:Lysine