[Gene transfer efficiency enhancement by inactivated Coxsakie virus B1 in primary skeletal muscle cells].
OBJECTIVE
To study the effect of inactivated Coxsackie virus B1(CVB1), which was considered to have high affinity to skeletal muscle cell, on gene transfer efficiency of reporter gene, pCDNA3-LacZ, in primary muscle cells.


METHODS
Viruses were purified by polyethylene sedimentation followed by sucrose supercentrifugation. Virus, transferrin-polylysine(TfpL) and LacZ were designed as different concentrations and groups, then positive cells contained beta-galactosidase were calculated as gene transfer efficiency.


RESULTS
Inactivated CVB1 made an enhancement of gene transfer efficiency from less than 1% in TfpL+LacZ group to 15-20 per cent in CVB1+TfpL+LacZ group.


CONCLUSIONS
Like adenovirus, inactivated CVB1 can also increase gene transfer efficiency in muscle cells, which provides experimental basis for constructing new vector targeting to muscle cells.
Chen
G
G
Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University of Medical Science, Guangzhou, 510080 P.R.China. gjchen@gzsums.edu.cn
Liu
Z
Z
Zhang
C
C
Jiang
L
L
Chen
Y
Y
Fang
D
D
Yan
H
H
D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000235:genetics*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D018482:Muscle, Skeletal / Q000166:cytology / Q000821:virology*