title: Contact guidance of rat fibroblasts on various implant materials.
abstract: Providing a substrate surface with micrometer-sized parallel grooves influences the behavior of cells growing on such substrates in vitro. Cells elongate in the direction of the groove and migrate guided by the grooves. It has been suggested that cellular alignment on microgrooves is predominantly dependent on groove dimensions and that surface chemical variation of the substrate material has little effect. Therefore we seeded primary rat dermal fibroblasts (RDF) on smooth and microgrooved (groove width 1-10 microm, depth 0.5 microm) polystyrene (PS), poly-L-lactic acid (PLA), silicone (SIL), and titanium (Ti) substrates. The production process was found to be more accurate for PS and PLA than for SIL and Ti substrates. A proliferation study, scanning electron microscopy, confocal laser scanning microscopy, and transmission electron microscopy revealed differences between RDF behavior on the materials. Our conclusions are (1) the accuracy of microtexture production by casting depends greatly on the material used; (2) even if no sharp discontinuities are present, microtextures still are potent tools for inducing contact guidance; and (3) besides surface texture, surface chemistry has a definitive influence on cell morphology.
authors.affiliation: University of Nijmegen, College of Dental Science, Department of Biomaterials, Faculty of Medical Sciences and Medical School, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: X F
authors.initials: XF
authors.lastname: Walboomers
authors.forename: H J
authors.initials: HJ
authors.lastname: Croes
authors.forename: L A
authors.initials: LA
authors.lastname: Ginsel
authors.forename: J A
authors.initials: JA
authors.lastname: Jansen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D002448:Cell Adhesion; D002455:Cell Division; D048429:Cell Size; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D019736:Prostheses and Implants / Q000009:adverse effects; D051381:Rats
chemical_list: D001672:Biocompatible Materials