The fusion protein of peste des petits ruminants virus is a hemolysin.
The fusion glycoprotein (F protein) of paramyxoviruses plays a vital role in virus-induced cytopathology. To explore the role of the F protein in peste des petits ruminants virus (PPRV)-induced cytopathology, the F protein of PPRV was purified by immunoaffinity chromatography. The purified F protein, when incubated with chicken erythrocytes, caused lysis suggesting that PPRV F protein is a hemolysin. Furthermore, the hemolysis can be inhibited by hyperimmune serum against F protein. The virus-induced cell fusion (syncytia) was also inhibited by the hyperimmune serum against the F protein. In summary, these results indicate that the purified PPRV F protein is biologically active and is involved in virus-induced hemolysis, cell-fusion and the initiation of infection.
Devireddy
L R
LR
Department of Veterinary Microbiology, Veterinary College, Bangalore, India.
Raghavan
R
R
Ramachandran
S
S
Shaila
M S
MS
D000818:Animals; D002846:Chromatography, Affinity; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006461:Hemolysis; D018107:Peste-des-petits-ruminants virus / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism*
D006460:Hemolysin Proteins; D014760:Viral Fusion Proteins