In vitro isolation of Neospora caninum from a stillborn calf in the UK.
Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR) techniques.
Davison
H C
HC
Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, Pembroke Place, Liverpool, L3 5QA, UK.
Guy
F
F
Trees
A J
AJ
Ryce
C
C
Ellis
J T
JT
Otter
A
A
Jeffrey
M
M
Simpson
V R
VR
Holt
J J
JJ
D000818:Animals; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002522:Chlorocebus aethiops; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D005313:Fetal Death / Q000469:parasitology* / Q000662:veterinary*; D018688:Neospora / Q000302:isolation & purification*; D019105:Random Amplified Polymorphic DNA Technique; D006113:United Kingdom; D014709:Vero Cells