Identification of a novel splice variant of heat shock cognate protein 70 after chronic antidepressant treatment in rat frontal cortex.
In this study, we identified a novel splice variant of 70-kDa heat shock cognate protein (HSC70), while screening differentially expressed molecules in rat brain after chronic antidepressant treatment. This clone, named HSC49, lacked 470 bp of nucleotides of rat HSC70. HSC49 encoded 442 amino acid residues with a calculated molecular mass of 48.6 kDa. DNA sequence analysis revealed that HSC49 lacked the entire Exon 7 and Exon 8 of the HSC70 gene. Chronic treatment with antidepressant, imipramine or sertraline, induced a 38.5 or 22.5% increase in mRNA levels in rat frontal cortex, respectively, when compared to controls. Western blot analysis also revealed that the protein expression of HSC49 was increased after antidepressant treatment. Our data suggest that HSC49 may be one of the common molecules induced after chronic antidepressant treatment.
Yamada
M
M
School of Pharmaceutical Sciences, Showa University, Tokyo, 142-8666, Japan.
Yamada
M
M
Kiuchi
Y
Y
Nara
K
K
Kanda
Y
Y
Morinobu
S
S
Momose
K
K
Oguchi
K
K
Kamijima
K
K
Higuchi
T
T
D017398:Alternative Splicing / Q000187:drug effects*; D000595:Amino Acid Sequence; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D014644:Genetic Variation; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D007099:Imipramine / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020280:Sertraline / Q000494:pharmacology
D000928:Antidepressive Agents; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494373:Hspa8 protein, rat; D012333:RNA, Messenger; D007099:Imipramine; D020280:Sertraline