title: Transmission trials, ITS2-PCR and RAPD-PCR show identity of Toxocara canis isolates from red fox and dog.
abstract: Toxocara canis isolates from dog and from red fox were compared in transmission trials and with molecular analysis using RAPD-PCR technique and comparison of the ITS2 sequence. After oral infection of bitches with 20,000 embryonated T. canis eggs of vulpine and canine origin, the vertical transmission to pup's was examined. All animals of both groups developed typical clinical symptoms of toxocarosis. The haematological, serological, parasitological and post mortem results showed no differences between both isolates except for the infectivity of T. canis stages in mice where the fox isolate showed a significant higher infectivity than the dog isolate. The RAPD-PCR showed a similarity coefficient of 0.95, similar to the range of intraspecific variation in Toxocara cati and Toxascaris leonina specimens as outgroups. The ITS2 comparison showed a 100% identity between both isolates with no intraspecific variations. Therefore, the study shows that the fox and the dog isolate of T. canis were identical in infectivity, transmission and molecular structure; a host adaptation could not be found and the fox has to be seen as a reservoir for T. canis infections in dogs. Considering the increasing number of foxes in urban areas the importance of helminth control in dogs is stressed.
authors.affiliation: Institute of Parasitology, Hannover School of Veterinary Medicine, Germany. cepe@parasit.tiho-hannover.de
authors.forename: C
authors.initials: C
authors.lastname: Epe
authors.forename: M
authors.initials: M
authors.lastname: Meuwissen
authors.forename: M
authors.initials: M
authors.lastname: Stoye
authors.forename: T
authors.initials: T
authors.lastname: Schnieder
mesh_terms: D000818:Animals; D001483:Base Sequence; D002415:Cats; D018509:DNA, Helminth / Q000737:chemistry; D004283:Dog Diseases / Q000469:parasitology / Q000635:transmission*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D005589:Foxes; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D017166:Toxocara canis / Q000737:chemistry / Q000235:genetics* / Q000472:pathogenicity; D014120:Toxocariasis / Q000635:transmission*
chemical_list: D018509:DNA, Helminth