[A sensitive method for quantitative analysis of hydrogen peroxide and oxidase substrates in biological samples].
The sensitive method for peroxidative assays of hydrogen peroxide (up to 5 nmole in 1 ml) and oxidases' substrates (glucose, ethanol) has been developed by use of a non-cancerogenic chromogen--3,3', 5,5'-tetramethylbenzidine (TMB), horseradish peroxidase and corresponding oxidases. The proposed modification of the known method consists in using decreased concentration of TMB (0.05-0.1 mM) and stopping the reaction by adding of acid to pH 1.4-2.1. This approach allowed to enhance the sensitivity of assays of corresponding analytes (7-10 times) and decrease the costs of analysis.
Honchar
M V
MV
Division of Cell Regulatory Systems, A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Lviv.
D001560:Benzidines / Q000378:metabolism; D002863:Chromogenic Compounds; D000431:Ethanol / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010088:Oxidoreductases
D001560:Benzidines; D002863:Chromogenic Compounds; C021758:3,3',5,5'-tetramethylbenzidine; D000431:Ethanol; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D006735:Horseradish Peroxidase; D005947:Glucose
Chutlyvnyĭ metod kil'kisnoho vyznachennia peroksydu vodniu ta substrativ oksydaz u biolohichnykh ob'ektakh.