Preferential binding of mouse mammary tumor virus to B lymphocytes.
Mouse mammary tumor virus (MMTV) has been shown to preferentially infect B lymphocytes in vivo. We have used recombinant envelope-coated fluospheres and highly purified MMTV particles to study the distribution of the viral receptors on fresh mouse lymphocytes. A preferential dose-dependent binding to B lymphocytes was observed which could be competed with neutralizing antibodies. In contrast, T-lymphocyte binding remained at background levels. These results strongly suggest a higher density of viral receptor molecules on B lymphocytes than on T lymphocytes and correlate with the preferential initial infection of B lymphocytes observed in vivo.
Baribaud
F
F
Institute of Microbiology, University of Lausanne, CH-1011 Lausanne, CH-1066 Epalinges, Switzerland. fbaribau@mail.med.upenn.edu
Shaw
A V
AV
Scarpellino
L
L
Diggelmann
H
H
Acha-Orbea
H
H
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D002478:Cells, Cultured; D008324:Mammary Tumor Virus, Mouse / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
D000957:Antigens, Viral, Tumor; D011993:Recombinant Fusion Proteins; C040612:glycoprotein 52 antigen, Mouse mammary tumor virus