Quantification of lamivudine (3TC) by competitive immunoassay.
Lamivudine or 3TC, the (-) eniantiomer of 2'-deoxy-3'-thiacytidine, is a prototype of a novel class of levogyre dideoxynucleosides analogues used in treatment of HIV and HBV infection. We describe a method corresponding to the first enzyme immunoassay for quantifying this antiviral drug. This technique use an enzyme conjugate that not require the use of radioactive labelling. In this study, anti-3TC antibodies were raised in rabbits by immunising with 3TC-HS-kelhoyle limpet hemocyanin (KLH) conjugate.
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Laboratoire de Chimie Bio-Organique, ESA CNRS 6001, Faculté des Sciences, Université de Nice-Sophia Antipolis, Nice, France.
Creminon
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Nevers
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MC
Grassi
J
J
Duval
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Guedj
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R
D019380:Anti-HIV Agents / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D007118:Immunoassay / Q000379:methods*; D019259:Lamivudine / Q000032:analysis* / Q000276:immunology; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis* / Q000276:immunology
D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D019259:Lamivudine