title: Oxidative protein folding is driven by the electron transport system.
abstract: Disulfide bond formation is catalyzed in vivo by DsbA and DsbB. Here we reconstitute this oxidative folding system using purified components. We have found the sources of oxidative power for protein folding and show how disulfide bond formation is linked to cellular metabolism. We find that disulfide bond formation and the electron transport chain are directly coupled. DsbB uses quinones as electron acceptors, allowing various choices for electron transport to support disulfide bond formation. Electrons flow via cytochrome bo oxidase to oxygen under aerobic conditions or via cytochrome bd oxidase under partially anaerobic conditions. Under truly anaerobic conditions, menaquinone shuttles electrons to alternate final electron acceptors such as fumarate. This flexibility reflects the vital nature of the disulfide catalytic system.
authors.affiliation: Department of Biology, University of Michigan, Ann Arbor 48109-1048, USA.
authors.forename: M
authors.initials: M
authors.lastname: Bader
authors.forename: W
authors.initials: W
authors.lastname: Muse
authors.forename: D P
authors.initials: DP
authors.lastname: Ballou
authors.forename: C
authors.initials: C
authors.lastname: Gassner
authors.forename: J C
authors.initials: JC
authors.lastname: Bardwell
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D003573:Cytochrome b Group; D003580:Cytochromes / Q000378:metabolism; D017931:DNA Primers; D004220:Disulfides / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology*; D045222:Electron Transport Chain Complex Proteins; D003576:Electron Transport Complex IV / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis / Q000502:physiology; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000032:analysis; D019704:Protein Disulfide-Isomerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003580:Cytochromes; D017931:DNA Primers; D004220:Disulfides; C079503:DsbB protein, Bacteria; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D014451:Ubiquinone; D010088:Oxidoreductases; C040077:cytochrome bd terminal oxidase complex, E coli; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV; D019704:Protein Disulfide-Isomerases; D010100:Oxygen