A first-generation porcine whole-genome radiation hybrid map.
A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod >/= 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score >/=4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of approximately 70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.
Hawken
R J
RJ
Program on Comparative Genomics, College of Veterinary Medicine, University of Minnesota, 1365 Gortner Avenue, St. Paul, Minnesota 55108, USA.
Murtaugh
J
J
Flickinger
G H
GH
Yerle
M
M
Robic
A
A
Milan
D
D
Gellin
J
J
Beattie
C W
CW
Schook
L B
LB
Alexander
L J
LJ
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D006822:Hybrid Cells; D016133:Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*
D005819:Genetic Markers