Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus.
A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples.
Bach
H J
HJ
Institute for Soil Ecology, GSF-National Research Center for Environment and Health, Neuherberg, Germany. Bach@gsf.de
Hartmann
A
A
Trevors
J T
JT
Munch
J C
JC
D001409:Bacillus cereus / Q000201:enzymology*; D006868:Hydrolysis; D007700:Kinetics; D008280:Magnetics; D008666:Metalloendopeptidases / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification*
D012333:RNA, Messenger; D008666:Metalloendopeptidases