Thromboxane A2-induced phosphatidylcholine hydrolysis in porcine vascular smooth muscle cells.
The effect of 9,11-epithio-11,12-methanothromboxane A2 (STA2), a thromboxane A2 receptor agonist, on phosphatidylcholine hydrolysis was examined in porcine vascular smooth muscle cells. Although STA2 stimulated diacylglycerol production in a concentration-dependent manner, it only caused a slight accumulation of [3H]phosphatidylethanol in the presence of 0.5% ethanol, reflecting its weak stimulation of phosphatidylcholine-specific phospholipase D. STA2-induced diacylglycerol production was potently and concentration dependently inhibited by potassium tricyclo-[5.2.1.0(2.6)]-decyl-(9[8])-xanthogenate (D609), an inhibitor of phosphatidylcholine-specific phospholipase C. These results suggest that the thromboxane A2 receptor in vascular smooth muscles is functionally coupled to phosphatidylcholine-specific phospholipase C to yield diacylglycerol.
Nakahata
N
N
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan. nakahata@mail.pharm.tohoku.ac.jp
Takano
H
H
Ohizumi
Y
Y
D000818:Animals; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D002478:Cells, Cultured; D004075:Diglycerides / Q000096:biosynthesis; D020404:Glycerophospholipids / Q000096:biosynthesis; D006868:Hydrolysis; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009636:Norbornanes; D010713:Phosphatidylcholines / Q000378:metabolism*; D017482:Receptors, Thromboxane / Q000502:physiology*; D013552:Swine; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology; D013928:Thromboxane A2 / Q000031:analogs & derivatives / Q000494:pharmacology
D001952:Bridged-Ring Compounds; D004075:Diglycerides; D020404:Glycerophospholipids; D009636:Norbornanes; D010713:Phosphatidylcholines; D017482:Receptors, Thromboxane; D013859:Thiocarbamates; D013871:Thiones; C051521:phosphatidylethanol; D013928:Thromboxane A2; C046498:tricyclodecane-9-yl-xanthogenate; C037866:STA 2