title: [Rapid detection of trisomy 21 by quantitative polymerase chain reaction].
abstract: OBJECTIVE
  Studying gene diagnosis of trisomy 21 by means of quantitative polymerase chain reaction(PCR).
  
  
  METHODS
  Polymorphic short tandem repeat(STR) at D21S11 served as the gene marker, and a pair of primers was synthesized. The samples from 11 normal subjects and 28 cases of trisomy 21 were analyzed by quantitative PCR.
  
  
  RESULTS
  10 normal subjects showed two bands with a ratio of 1:1, and a subject showed one band. Of 28 cases of trisomy 21, 24 cases showed two bands with a ratio of 2:1, 3 cases showed three bands with a ratio of 1:1:1, and 1 case showed one band.
  
  
  CONCLUSIONS
  Polymorphic STR at D21S11 is a valuable gene marker for diagnosis of trisomy 21. Trisomy 21 can be diagnosed rapidly and accurately within 24 hours by quantitative PCR.
authors.affiliation: Department of Medical Genetics, China Medical University, Shenyang 110001 P.R.China. Klsun@IRIS.CMU.EDU.CN
authors.forename: C
authors.initials: C
authors.lastname: Jin
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: C
authors.initials: C
authors.lastname: Lin
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Sun
mesh_terms: D004314:Down Syndrome / Q000175:diagnosis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*