title: Daily copper supplement effects on copper balance in trained subjects during prolonged restriction of muscular activity.
abstract: The aim of this study was to assess the effect of a daily intake of copper supplements on negative copper balance during prolonged exposure to hypokinesia (decreased number of kilometers per day). During hypokinesia (HK), negative copper balance is shown by increased, not by decreased, serum copper concentration, as it happens in other situations. Studies were done during a 30-d prehypokinetic period and a 364-d hypokinetic period. Forty male trained volunteers aged 22-26 yr with a peak oxygen uptake of 66.4 mL/min/kg and with an average of 13.7 km/d running distance were chosen as subjects. They were equally divided into four groups: unsupplemented ambulatory control subjects (UACS), unsupplemented hypokinetic subjects (UHKS), supplemented hypokinetic subjects (SHKS), and supplemented ambulatory control subjects (SACS). The SACS and SHKS groups took 0.09 mg copper carbonate/kg body weight daily. The SHKS and UHKS groups were maintained under an average running distance of 1.7 km/d, whereas the SACS and UACS groups did not experience any modifications in their normal training routines. During the 30-d prehypokinetic period and the 346-d hypokinetic period, urinary excretion of copper, calcium, and magnesium and serum concentrations of copper, calcium, and magnesium were measured. Copper loss in feces and copper balance was also determined. In both UHKS and SHKS groups, urinary excretion of copper, calcium, and magnesium and concentrations of copper, magnesium, and calcium in serum increased significantly when compared with the SACS and UACS groups. Loss of copper in feces was also increased significantly in the SHKS and UHKS groups when compared with the UACS and SACS groups. Throughout the study, the copper balance was negative in the SHKS and UHKS groups, whereas in the SACS and UACS groups, the copper balance was positive. It was concluded that a daily intake of copper supplements cannot be used to prevent copper deficiency shown by increased copper concentration. Copper supplements also failed to prevent negative copper balance and copper losses in feces and urine in endurance-trained subjects during prolonged exposure to HK.
authors.affiliation: Hypokinetic Physiology Laboratory, European Institute of Environmental Cybernetics, Athens, Greece.
authors.forename: Y G
authors.initials: YG
authors.lastname: Zorbas
authors.forename: K P
authors.initials: KP
authors.lastname: Charapakin
authors.forename: V J
authors.initials: VJ
authors.lastname: Kakurin
authors.forename: N K
authors.initials: NK
authors.lastname: Kuznetsov
authors.forename: M A
authors.initials: MA
authors.lastname: Federov
authors.forename: V K
authors.initials: VK
authors.lastname: Popov
mesh_terms: D000328:Adult; D000886:Anthropometry; D003300:Copper / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D015444:Exercise; D005243:Feces / Q000737:chemistry; D006801:Humans; D018476:Hypokinesia / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism
chemical_list: D003300:Copper; D010100:Oxygen