title: Murine leukemia provirus-mediated activation of the Notch1 gene leads to induction of HES-1 in a mouse T lymphoma cell line, DL-3.
abstract: Constitutive activation of Notch signaling is known to be associated with tumorigenesis. In a mouse T lymphoma cell line, DL-3, we found that a murine leukemia provirus was inserted in the Notch1 locus, which led to marked expression of a virus-Notch1 fusion mRNA encoding an intracellular portion of the Notch1 protein. Furthermore, expression and nuclear localization of this constitutively active form of Notch1 protein were confirmed. Corresponding to this finding, the transcription of the hairy/enhancer of split (HES-1) gene, a known target of Notch1 signaling, was elevated in this cell line. A potential role for overexpressed HES-1 in the development of the lymphoma was discussed.
authors.affiliation: Department of Viral Oncology, Institute for Virus Research, Kyoto University, Japan.
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Ishimoto
authors.forename: T
authors.initials: T
authors.lastname: Honjo
authors.forename: S
authors.initials: S
authors.lastname: Yanagawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D015321:Gene Rearrangement; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000821:virology; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics* / Q000472:pathogenicity; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D004279:DNA, Viral; D018398:Homeodomain Proteins; D008565:Membrane Proteins; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; C489734:Hes1 protein, mouse; C497762:Notch1 protein, mouse