Gas chromatographic determination of galactose in milk. Example of a switching valve used for the protection of the capillary column.
Galactose, a marker of heat treatment, has been analysed in milk as pentafluorobenzyloxime acetate by gas chromatography with flame ionization detection using a simple switching valve system. The procedure did not entail any prederivatization clean-up for lactose elimination from the sample. In a short pre-column, reagent and lactose derivative excess were separated and the galactose and internal standard derivatives were transferred to the analytical column by a four-port valve. Thus, the analytical column was protected from overloading, so avoiding rapid deterioration.
Chiesa
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LM
Istituto di Fisiologia Veterinaria e Biochimica, Milan, Italy.
Radice
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L
Belloli
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Renon
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Biondi
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PA
D000818:Animals; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005690:Galactose / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
D005690:Galactose