title: Characteristics of recombinant human butyrylcholinesterase.
abstract: OBJECTIVE
  To study the biochemical-pharmacological properties of the recombinant human butyrylcholinesterase (rhBChE) and thereby to size up the potential possibility of using it as a detoxifying agent in succinylcholine intoxication.
  
  
  METHODS
  CHO-dhfr cells were transfected with plasmids by electroporation. BChE activity was determined colorimetrically by 5, 5'-dithiobis-(2-nitrobenzoic acid) (DTNB) method. Antigenicity was estimated by enzyme-linked immunosorbent assay and Western blot.
  
  
  RESULTS
  The maximal expression amounted to 25.83 ng.h-1/10(6) cells. The rhBChE was highly similar to the native human BChE (nhBChE) in terms of its catalytic property, substrate affinity, inhibitor sensitivity, reactivation, stability, and immunoreactivity with anti-nhBChE antibodies. Mice challenged with 1.5 lethal dose of succinylcholine preincubated with rhBChE survived without any symptoms of intoxication.
  
  
  CONCLUSIONS
  The rhBChE and nhBChE exhibit similar biochemical-pharmacological features. It is of potential value in practical use.
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Yuan
authors.forename: W Y
authors.initials: WY
authors.lastname: Yu
authors.forename: C H
authors.initials: CH
authors.lastname: Shi
authors.forename: M J
authors.initials: MJ
authors.lastname: Sun
mesh_terms: D000818:Animals; D002091:Butyrylcholinesterase / Q000235:genetics* / Q000378:metabolism* / Q000627:therapeutic use; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D018274:Electroporation; D051379:Mice; D010957:Plasmids; D011041:Poisoning / Q000517:prevention & control; D011994:Recombinant Proteins / Q000378:metabolism / Q000627:therapeutic use; D013390:Succinylcholine / Q000506:poisoning; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D002091:Butyrylcholinesterase; D013390:Succinylcholine