title: The role of B/T costimulatory signals in the immunopotentiating activity of neisserial porin.
abstract: A T cell-dependent immune response to group C meningococcal capsular polysaccharide (CPS) can be elicited when CPS is conjugated to the class 3 neisserial porin (CPS-porin). Treatment of CPS-porin-immunized mice with B7-2 blocking monoclonal antibody (MAb) caused a dramatic reduction in the CPS-specific IgG response, treatment with anti-B7-1 MAb had no effect, and concurrent blockade of B7-1 and B7-2 resulted in a synergistic abrogation of the CPS-specific IgG response while the CPS IgM response was unaffected. Anti-CD40L MAb treatment caused a significant reduction of both CPS-specific IgG and IgM levels. In contrast, blockade of CTLA4 interactions resulted in increases in both CPS IgG and IgM responses in CPS-porin-immunized mice. These data support the hypothesis that the ability of neisserial porins to improve the immune response to poorly immunogenic antigens (e.g., polysaccharides) is related to porin-induced increases in B7-2 expression on antigen-presenting cells and enhanced B/T cell interactions.
authors.affiliation: Maxwell Finland Laboratory for Infectious Diseases, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
authors.forename: F G
authors.initials: FG
authors.lastname: Mackinnon
authors.forename: Y
authors.initials: Y
authors.lastname: Ho
authors.forename: M S
authors.initials: MS
authors.lastname: Blake
authors.forename: F
authors.initials: F
authors.lastname: Michon
authors.forename: A
authors.initials: A
authors.lastname: Chandraker
authors.forename: M H
authors.initials: MH
authors.lastname: Sayegh
authors.forename: L M
authors.initials: LM
authors.lastname: Wetzler
mesh_terms: D000069594:Abatacept; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000917:Antibody Formation; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D060908:CTLA-4 Antigen; D007115:Immunization Schedule; D018796:Immunoconjugates; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D009344:Neisseria gonorrhoeae / Q000276:immunology; D009345:Neisseria meningitidis / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D018272:Porins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D001428:Bacterial Vaccines; D060908:CTLA-4 Antigen; C556707:Ctla4 protein, mouse; D018796:Immunoconjugates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial; D018272:Porins; C057699:porin protein, Neisseria; D000069594:Abatacept