title: Crosslinking of enzymes for improved stability and performance.
abstract: Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1-200 microm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit increased storage stability as well as stability in applications. The technology is complementary to protein engineering methods that aim at boosting the inherent stability of the enzyme.
authors.affiliation: Altus Biologics, 625 Putnam Ave, Cambridge, MA 02139, USA. govardhan@altus.com
authors.forename: C P
authors.initials: CP
authors.lastname: Govardhan
mesh_terms: D001709:Biotechnology; D003432:Cross-Linking Reagents; D003460:Crystallization; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D066298:In Vitro Techniques; D015202:Protein Engineering
chemical_list: D003432:Cross-Linking Reagents; D004798:Enzymes