Characterization of the mouse collectin gene locus.
Three of the four known mouse collectin genes have been mapped to chromosome 14. To further characterize the spatial relationship of these genes, a bacterial artificial chromosome (BAC) library of mouse chromosome 14 was screened using mouse surfactant protein (SP)-A and -D complementary DNAs (cDNAs). One large clone hybridized to both SP-A and SP-D cDNAs and was found by polymerase chain reaction (PCR) to contain sequences from one of the mouse mannose-binding lectin genes (Mbl1). We used Southern mapping and subcloning of overlapping restriction fragments to characterize the gene locus. Mapping was confirmed by fluorescent in situ hybridization of fiber-stretched BAC DNA and by Southern hybridization of restriction endonuclease-digested and PCR-amplified genomic DNA. We found that the SP-A, Mbl1, and SP-D genes reside contiguously within a 55-kb region. The SP-A and Mbl1 genes are in the same 5' to 3' orientation and 16 kb apart. The SP-D gene is in the opposite orientation to the two other collectin genes, 13 kb away from the 3' end of the Mbl1 gene. The mouse SP-D gene had not previously been characterized. We found its size (13 kb) and organization to be similar to that of human SP-D. Exon I is untranslated. The second exon is a hybrid exon that contains signal for initiation of translation, signal peptide, N-terminal domain, and the first seven collagen triplets of the collagen-like domain of the protein. Four short exons (III through VI) encode the collagen-like domain of the protein, and exons VII and VIII the linking and the carbohydrate-recognition domains, respectively.
Akiyama
J
J
Cardiovascular Research Institute and Department of Pediatrics, University of California San Francisco, San Francisco, USA.
Volik
S V
SV
Plajzer-Frick
I
I
Prince
A
A
Sago
H
H
Weier
H U
HU
Vanderbilt
J N
JN
Hawgood
S
S
Poulain
F R
FR
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D037222:Collectins; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons; D015698:Genomic Library; D006023:Glycoproteins / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D037601:Mannose-Binding Lectin / Q000031:analogs & derivatives*; D037241:Mannose-Binding Lectins; D051379:Mice / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011510:Proteolipids / Q000235:genetics; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000235:genetics
D002352:Carrier Proteins; D037222:Collectins; D018076:DNA, Complementary; D006023:Glycoproteins; D037601:Mannose-Binding Lectin; D037241:Mannose-Binding Lectins; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; C069858:mannose binding protein A