Flow cytometric analysis of thymocyte subpopulations in mice after whole-body X-irradiation.
To determined the cellular kinetics of thymocyte subpopulations in DBA1 mice after whole-body 6.8 Gy X-irradiation, they were analyzed for the expression of several cell surface antigens using flow cytometry. The results show that i) The majority of thymocytes rapidly depleted by irradiation was CD4+8+ cells. ii) radioresistant CD4+8- and CD4-8+ survived 18-48 hr after X-irradiation were considered to be relatively mature type, since they expressed high levels of CD3 and LECAM-1. iii) CD3-positive cells were detected in CD4-8- cells at 72 hr after irradiation.
Oka
S
S
Department of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Japan.
Kubo
K
K
Matsuyama
S
S
Takamori
Y
Y
D000818:Animals; D000954:Antigens, Surface / Q000528:radiation effects*; D016516:CD4-CD8 Ratio / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008811:Mice, Inbred DBA; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000528:radiation effects*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects; D014916:Whole-Body Irradiation
D000954:Antigens, Surface