Synthesis of probes for the active site of leucine dehydrogenase.
A series of novel 3-substituted 2-oxobutanoic acids were prepared and incubated with leucine dehydrogenase giving in one case both a kinetic resolution at C-3 and reductive amination of the ketone. This is the first example of an amino acid dehydrogenase catalysed kinetic resolution and reductive amination.
Sutherland
A
A
School of Chemistry, University of Bristol, Cantock's Close, UK.
Willis
C L
CL
D000594:Amino Acid Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D050803:Leucine Dehydrogenase; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D015335:Molecular Probes / Q000138:chemical synthesis*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013912:Threonine / Q000138:chemical synthesis
D015335:Molecular Probes; D013912:Threonine; D000594:Amino Acid Oxidoreductases; D050803:Leucine Dehydrogenase; D008049:Lipase