JIL-1: a novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila.
We have cloned and characterized JIL-1, a novel tandem kinase in Drosophila that associates with the chromosomes throughout the cell cycle. Antibody staining and live imaging of JIL-1-GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost 2-fold on the hypertranscribed male X chromosome compared to autosomes. Phylogenetic analysis suggests that JIL-1 together with human MSKs defines a separate family of tandem kinases. That JIL-1 is a functional kinase was demonstrated by autophosphorylation and phosphorylation of histone H3 in vitro. Based on these findings, we propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure.
Jin
Y
Y
Department of Zoology and Genetics, Iowa State University, Ames 50011, USA.
Wang
Y
Y
Walker
D L
DL
Dong
H
H
Conley
C
C
Johansen
J
J
Johansen
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D002453:Cell Cycle; D002843:Chromatin / Q000737:chemistry; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004330:Drosophila / Q000196:embryology / Q000201:enzymology*; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D012469:Salivary Glands / Q000201:enzymology; D014960:X Chromosome / Q000235:genetics
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D019476:Insect Proteins; D011494:Protein Kinases