The role of high-resolution structural studies in the development of commercial enzymes.
Recent developments in both NMR and X-ray crystallography allow the analysis of commercial enzymes in unprecedented detail. The novel methods provide detailed insights into protein dynamics, establish the existence of special catalytic hydrogen bonds and define the ionization states at the enzyme active site. A more detailed understanding of how the changes in structure are related to altered function should facilitate the design of future commercial enzymes with improved performance for different environmental conditions.
Bott
R
R
Genencor International Inc., 925 Page Mill Road, Palo Alto, CA 94304-1013, USA. rbott@genencor.com
Boelens
R
R
D001709:Biotechnology; D018360:Crystallography, X-Ray / Q000379:methods; D004798:Enzymes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011487:Protein Conformation
D004798:Enzymes