title: Immunohistological and ultrastructural analysis of the intimal thickening in coarctation of human aorta.
abstract: OBJECTIVE
  The histological nature and characteristics of aortic coarctation are not clearly defined, the aim of this study is to analyse intimal thickening in aortic coarctation.
  
  
  METHODS
  In order to characterize the components of intimal thickening in coarctation, narrowed segments of aorta obtained after surgery from ten children were examined immunocytochemically and by electron microscopy.
  
  
  RESULTS
  Histological analysis of aortic coarctation demonstrated a widened subendothelial region with separation of endothelial cells from the internal elastic lamina. Masson's trichrome staining showed a marked increase in extracellular matrix and cell numbers in the intimal thickening compared with normal aorta. Cellular component analysis demonstrated invagination of the intima by smooth muscle actin-positive cells, with a fragmentation of the internal elastic lamina. No proliferating smooth muscle and inflammatory cells were identified in the intima. In order to characterize the smooth muscle cell phenotypes, various smooth muscle cell markers were sought using specific monoclonal antibodies: alpha-smooth muscle actin, smooth muscle-myosin heavy chain, heavy caldesmon, desmin. In moderate coarcted aorta, at least two distinct smooth muscle phenotypes were identified. In the juxtamedial part of the intima smooth muscle, cells were differentiated and expressed all smooth muscle markers; in the subendothelial part of the intimal thickening, the majority of smooth muscle cells expressed only alpha-smooth muscle actin and appeared dedifferentiated. In regions of marked stenosis, a strong expression of smooth muscle-myosin heavy chain, and heavy caldesmon in the intimal thickening pointed to the presence of redifferentiated smooth muscle cells, not still expressing desmin. Electron microscopic examination also revealed a variety of smooth muscle cell phenotypes in the intimal thickening. In the superficial layer, smooth muscle cells appeared to be in the synthetic state, while in the deeper part, both synthetic and contractile components were identified.
  
  
  CONCLUSIONS
  These observations indicated that human coarctation was characterized by intimal recruitment of non-proliferating smooth muscle cells with dedifferentiated phenotype. However, the presence of smooth muscle cells with an intermediate phenotype in the narrowest part of the coarctation suggest that the redifferentiation process could participate in the pathogenesis of aortic coarctation.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 441 de Cardiologie, Pessac, France.
authors.forename: M
authors.initials: M
authors.lastname: Jimenez
authors.forename: D
authors.initials: D
authors.lastname: Daret
authors.forename: A
authors.initials: A
authors.lastname: Choussat
authors.forename: J
authors.initials: J
authors.lastname: Bonnet
mesh_terms: D000199:Actins / Q000032:analysis; D001011:Aorta / Q000737:chemistry / Q000648:ultrastructure; D001017:Aortic Coarctation / Q000378:metabolism / Q000473:pathology*; D015415:Biomarkers / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D002454:Cell Differentiation; D002675:Child, Preschool; D003893:Desmin / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000648:ultrastructure; D017539:Tunica Intima / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D000199:Actins; D015415:Biomarkers; D002148:Calmodulin-Binding Proteins; D003893:Desmin