title: Proteoglycan changes in the extracellular matrix of lung tissue from patients with pulmonary emphysema.
abstract: To characterize the changes in the extracellular matrix in smoking-related pulmonary emphysema, we undertook immunohistochemical studies in lung tissues from controls (n = 7), from patients with mild (n = 11) and severe (n = 8) emphysema, and from patients with lung fibrosis (n = 6). We studied collagens, laminin, fibronectin, proteoglycans (PGs), and beta1-integrins. The majority of the patients with severe emphysema showed diminished staining for the interstitial PGs, decorin and biglycan, in the peribronchiolar area, compared with patients in the control and fibrosis groups. Only a minority of patients with mild emphysema showed this diminished staining. In contrast, decorin and biglycan were well preserved in the perivascular area of all of the specimens from the emphysema group. Heparan sulfate PG staining was diminished in the respiratory airspace walls of patients with emphysema and fibrosis. Staining for Types I, III, and IV collagen, as well as for laminin, fibronectin, and the integrins, showed no differences between the four groups. The specific loss of interstitial PGs may be crucial for elastic recoil loss and subsequent bronchiolar obstruction, as seen in patients with smoking-related emphysema.
authors.affiliation: Department of Pathology, University of Groningen, The Netherlands. j.van.straaten@path.azg.nl
authors.forename: J F
authors.initials: JF
authors.lastname: van Straaten
authors.forename: W
authors.initials: W
authors.lastname: Coers
authors.forename: J A
authors.initials: JA
authors.lastname: Noordhoek
authors.forename: S
authors.initials: S
authors.lastname: Huitema
authors.forename: J T
authors.initials: JT
authors.lastname: Flipsen
authors.forename: H F
authors.initials: HF
authors.lastname: Kauffman
authors.forename: W
authors.initials: W
authors.lastname: Timens
authors.forename: D S
authors.initials: DS
authors.lastname: Postma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D058578:Biglycan; D003094:Collagen / Q000032:analysis; D058575:Decorin; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D005260:Female; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000032:analysis; D007797:Laminin / Q000032:analysis; D008168:Lung / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism*; D011656:Pulmonary Emphysema / Q000378:metabolism* / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000378:metabolism / Q000473:pathology
chemical_list: C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019012:Integrin beta1; D007797:Laminin; D011509:Proteoglycans; D003094:Collagen