title: Efficacy of monoclonal antibody 131I-B72.3 immunoscintigraphy of pancreatic adenocarcinoma xenografts in nude mice.
abstract: OBJECTIVE
  To assess the efficacy of monoclonal antibody (MoAb) B72.3 for in vivo-immunoscintigraphy of pancreatic carcinoma in nude mice.
  
  
  METHODS
  Experimental controlled animal study.
  
  
  METHODS
  University hospital, The Netherlands.
  
  
  METHODS
  11 nude mice with subcutaneously xenografted human pancreatic carcinoma.
  
  
  METHODS
  Specific MoAb B72.3 and non-specific MoAb MOPC21 were iodinated with 131I and injected intraperitoneally in nude mice. Scintigrams were taken on days 1-10 and tumour:non-tumour ratios of the regions of interest (tumour, thorax, abdomen, background) were calculated. The mice were then killed for in vitro tissue counts.
  
  
  METHODS
  Tumour:non-tumour ratios in vivo and in vitro.
  
  
  RESULTS
  Results of immunoscintigraphy on days 1, 2, and 6 were compared. In the B72.3-group all ratios were only moderately raised, the tumour:background ratio being the highest (2.35 (SD 0.67)) on day 6. There were no obvious differences between the ratios of the B72.3-group and the MOPC21-group. The results of tissue counts done at the end of the study, showed that tumour:non-tumour ratios were twice as high in the B72.3-group, suggesting some specificity of this MoAb.
  
  
  CONCLUSIONS
  The results of our study suggest that MoAb B72.3 is not powerful enough for in vivo detection of pancreatic cancer as assessed in this xenograft model in nude mice.
authors.affiliation: Department of Surgery, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: L W
authors.initials: LW
authors.lastname: Kaufmann
authors.forename: J C
authors.initials: JC
authors.lastname: Vaillant
authors.forename: T M
authors.initials: TM
authors.lastname: van Gulik
authors.forename: E A
authors.initials: EA
authors.lastname: van Royen
authors.forename: R
authors.initials: R
authors.lastname: Parc
authors.forename: H
authors.initials: H
authors.lastname: Obertop
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging*; D000818:Animals; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000918:Antibody Specificity; D004353:Drug Evaluation, Preclinical; D015902:Gamma Cameras; D006801:Humans; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging*; D016719:Radioimmunodetection / Q000379:methods* / Q000706:statistics & numerical data; D018709:Statistics, Nonparametric; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; C091202:B72.3 antibody; D007457:Iodine Radioisotopes; C121421:MOPC21 monoclonal antibody