title: A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4.
abstract: The ubiquitin-conjugating enzyme UbcM4 was previously shown to be necessary for normal mouse development. As a first step in identifying target proteins or proteins involved in the specificity of UbcM4-mediated ubiquitylation, we have isolated seven cDNAs encoding proteins that specifically interact with UbcM4 but with none of the other Ubcs tested. This interaction was observed in yeast as well as in mammalian cells. With one exception, all UbcM4-interacting proteins (UIPs) belong to a family of proteins that contain a RING finger motif. As they are structurally related to RING finger proteins that have recently been shown to play an essential role in protein ubiquitylation and degradation, the possibility is discussed that UIPs are involved in the specific recognition of substrate proteins of UbcM4.
authors.affiliation: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Martinez-Noel
authors.forename: R
authors.initials: R
authors.lastname: Niedenthal
authors.forename: T
authors.initials: T
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Harbers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D012441:Saccharomyces cerevisiae; D016415:Sequence Alignment; D013379:Substrate Specificity; D044763:Ubiquitin-Conjugating Enzymes; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; C117188:Ube2l3 protein, mouse; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases