Separation of double-stranded and single-stranded DNA in polymer solutions: II. Separation, peak width and resolution.
The electrophoretic separation of single-stranded and double-stranded DNA has been examined, using a matrix of linear poly-N,N-dimethylacrylamide (pDMA). The dependence of peak spacing, peak width and resolution on important parameters such as polymer concentration, polymer chain length and electric field strength, has been studied. This work complements our systematic study on electrophoretic mobility under different conditions (C. Heller, Electrophoresis 1999, 20, 1962-1977), and will help to further optimize and improve high performance DNA separation in capillary electrophoresis with entangled polymer solutions.
Heller
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Abteilung Lehrach, Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany. heller@mpimg-berlin-dahlem.mpg.de
D000178:Acrylamides / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011108:Polymers; D012996:Solutions
D000178:Acrylamides; D004277:DNA, Single-Stranded; D011108:Polymers; D012996:Solutions; D004247:DNA; C099046:N,N-dimethylacrylamide