title: DsbA is required for stable expression of outer membrane protein YscC and for efficient Yop secretion in Yersinia pestis.
abstract: The role of the periplasmic disulfide oxidoreductase DsbA in Yop secretion was investigated in Yersinia pestis. A Y. pestis dsbA mutant secreted reduced amounts of the V antigen and Yops and expressed reduced amounts of the full-sized YscC protein. Site-directed mutagenesis of the four cysteine residues present in the YscC protein resulted in defects similar to those found in the dsbA mutant. These results suggest that YscC contains at least one disulfide bond that is essential for the function of this protein in Yop secretion.
authors.affiliation: Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida 33176, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Jackson
authors.forename: G V
authors.initials: GV
authors.lastname: Plano
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D017931:DNA Primers; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020125:Mutation, Missense; D019704:Protein Disulfide-Isomerases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C094424:YscC protein, Yersinia pestis; C060772:yopM protein, Yersinia; C070015:YopN protein, Yersinia; D019704:Protein Disulfide-Isomerases