Lipid peroxidation and antioxidant status in experimental diabetes.
Oxidative stress is currently suggested as a mechanism underlying diabetes. The present study was designed to evaluate the oxidative stress related parameters in streptozotocin-induced diabetes in rats using different complementary approaches: susceptibility to in vitro oxidation (lipid peroxidation induction in liver homogenate, red blood cells hemolysis), blood antioxidant status (total antioxidant capacity by two approaches), and plasma isoprostane measurement, a new marker of lipid peroxidation in vivo. We have shown that induced liver thiobarbituric acid reactive substances increased after 4 weeks of diabetes, in spite of increased liver vitamin E content. Red blood cells hemolysis was significantly delayed after 4 weeks of diabetes. Plasma antioxidant capacity (AOC) tended to increase after 4 weeks of diabetes and was correlated with plasma vitamin E levels. Total antioxidant activity (TAA) significantly decreased after 1 week and a significant correlation was observed with plasma albumin levels. Plasma isoprostane (8-epiprostaglandinF2alpha) concentrations were not modified significantly 1 week or 4 weeks after the induction of diabetes. Levels of vitamin E in the diet and changes in its distribution among the body seems to play an important role in the development of oxidative stress during diabetes and its consequences.
Feillet-Coudray
C
C
Centre de Recherche en Nutrition Humaine d'Auvergne, Unité des Maladies Métaboliques et des Micronutriments, INRA, St. Genès Champanelle, France. feillet@clermont.inra.fr
Rock
E
E
Coudray
C
C
Grzelkowska
K
K
Azais-Braesco
V
V
Dardevet
D
D
Mazur
A
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016718:Arachidonic Acid / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D006461:Hemolysis; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014280:Triglycerides / Q000378:metabolism
D000975:Antioxidants; D014280:Triglycerides; C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; D013311:Streptozocin; D015237:Dinoprost