title: A standardized method of identification of raw and heat-processed fish by urea isoelectric focusing: a collaborative study.
abstract: A urea-isoelectric focusing (urea-IEF) method of identifying fish species in processed fishery products was investigated as an interlaboratory collaborative study. The technique was optimized with respect to (i) protein extraction conditions, composition of the extraction solution (urea and SDS solutions), determination of protein concentrations of the fish extracts (five tested methods); (ii) nature of gel (with carrier ampholytes and Immobilines), conditions of rehydration of commercial dry gels, urea concentration; (iii) staining conditions, Coomassie blue and silver staining. The results of various experiments were compared to select the most appropriate methodology, with respect to the discrimination power of differentiating species with the minimal influence of heat processing, reproducibility, speed, and ease of application. The method recommended meets the requirements of food control and customs laboratories.
authors.affiliation: IFREMER, Nantes, France. monique.etienne@ifremer.fr
authors.forename: M
authors.initials: M
authors.lastname: Etienne
authors.forename: M
authors.initials: M
authors.lastname: Jérôme
authors.forename: J
authors.initials: J
authors.lastname: Fleurence
authors.forename: H
authors.initials: H
authors.lastname: Rehbein
authors.forename: R
authors.initials: R
authors.lastname: Kundiger
authors.forename: I M
authors.initials: IM
authors.lastname: Yman
authors.forename: M
authors.initials: M
authors.lastname: Ferm
authors.forename: A
authors.initials: A
authors.lastname: Craig
authors.forename: I
authors.initials: I
authors.lastname: Mackie
authors.forename: F
authors.initials: F
authors.lastname: Jessen
authors.forename: A
authors.initials: A
authors.lastname: Smelt
authors.forename: J
authors.initials: J
authors.lastname: Luten
mesh_terms: D005396:Fish Products / Q000032:analysis*; D005511:Food Handling; D006358:Hot Temperature; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D011506:Proteins / Q000032:analysis; D012758:Shellfish / Q000032:analysis; D016622:Silver Staining; D014508:Urea / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D014508:Urea