Resistivity of red blood cells against high-intensity, short-duration electric field pulses induced by chelating agents.
The interaction of human red blood cells (RBCs) with diethylenetriamine-pentaacetic acid (DTPA) or its Gd-complex (Magnevist, a widely used clinical magnetic resonance contrast agent containing free DTPA ligands) led to the following, obviously interrelated phenomena. (i) Both compounds protected erythrocytes against electrohemolysis in isotonic solutions caused by a high-intensity DC electric field pulse. (ii) The inhibition of electrohemolysis was observed only when cells were electropulsed in low-conductivity solutions. (iii) The uptake of Gd-DTPA by electropulsed RBCs was relatively low. (iv) (Gd-) DTPA reduced markedly deformability of erythrocytes, as revealed by the electrodeformation experiments using high-frequency electric fields. Taken together, the results indicate that (Gd-) DTPA produce stiffer erythrocytes that are more resistant to electric field exposure. The observed effects of the chelating agents on the mechanical properties and the electropermeabilization of RBCs must have an origin in molecular changes of the bilayer or membrane-coupled cytoskeleton, which, in turn, appear to result from an alteration of the ionic equilibrium (e.g., Ca(2+) sequestration) in the vicinity of the cell membrane.
Mussauer
H
H
Lehrstuhl für Biotechnologie, Biozentrum, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
Sukhorukov
V L
VL
Haase
A
A
Zimmermann
U
U
D002614:Chelating Agents / Q000494:pharmacology*; D004548:Elasticity / Q000187:drug effects; D017097:Electric Impedance; D018274:Electroporation; D004907:Erythrocyte Deformability / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005682:Gadolinium; D019786:Gadolinium DTPA / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D004369:Pentetic Acid / Q000494:pharmacology*
D002614:Chelating Agents; D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA