Effects of anabolic steroid (19-nortestosterone) on the secretion of testicular hormones in the stallion.
The aim of this study was to clarify the effect of anabolic steroids on the testicular endocrine function of mature stallions. Mature thoroughbred stallions were treated with 800 mg nandrolone decanoate every 3 weeks for 3 months. After the first treatment, plasma concentrations of LH, immunoreactive inhibin and testosterone decreased rapidly to the nadir. These hormones were maintained at significantly lower concentrations compared with concentrations in intact stallions. Histology of the testicular tissue indicated the arrest of advanced spermatogenesis in the seminiferous tubules and a severe depletion of the number of Leydig cells in the interstitial compartment as a result of treatment. Most of the immunopositive cells for the inhibin alpha-subunit and steroidogenesis enzymes in the interstitial compartment decreased below detectable amounts, whereas immunopositive reactions of inhibin alpha-subunit in the seminiferous tubules were clearly observed. In conclusion, the treatment of mature stallions with nandrolone decanoate caused a decrease in the secretion of ir-inhibin and testosterone from the testis, the depletion of the number of Leydig cells and a decrease below detectable amounts of inhibin alpha-subunit and steroidogenesis enzymes. The concentration of ir-inhibin in the peripheral blood may be a useful marker for the examination of testicular activity in stallions being treated with anabolic steroids.
Nagata
S
S
Laboratory of Racing Chemistry, Tochigi, Japan.
Kurosawa
M
M
Mima
K
K
Nambo
Y
Y
Fujii
Y
Y
Watanabe
G
G
Taya
K
K
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis; D045930:Anabolic Agents / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D001141:Aromatase / Q000032:analysis; D015415:Biomarkers / Q000097:blood; D002452:Cell Count; D003864:Depression, Chemical; D005640:Follicle Stimulating Hormone / Q000097:blood; D006736:Horses / Q000378:metabolism*; D007265:Inhibins / Q000097:blood*; D007985:Leydig Cells / Q000737:chemistry / Q000187:drug effects; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077603:Nandrolone Decanoate; D010455:Peptides / Q000097:blood; D011863:Radioimmunoassay; D012708:Sertoli Cells / Q000737:chemistry / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D018709:Statistics, Nonparametric; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*
D045930:Anabolic Agents; D015415:Biomarkers; D010455:Peptides; C094377:inhibin-alpha subunit; D013739:Testosterone; D007265:Inhibins; D009277:Nandrolone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015096:3-Hydroxysteroid Dehydrogenases; D001141:Aromatase; D000077603:Nandrolone Decanoate