The intranuclear movement of Balbiani ring premessenger ribonucleoprotein particles.
Specific premessenger ribonucleoprotein (pre-mRNP) particles, the Balbiani ring (BR) granules in the salivary glands of the dipteran Chironomus tentans, can be visualized in the electron microscope when they assemble on the genes, move through nucleoplasm, and bind to and translocate through the nuclear pores. As shown by BrUTP labeling and immunoelectron microscopy, newly synthesized BR RNP particles, released from the BR genes, appear early in all nucleoplasmic regions of the cell nucleus and they saturate the nucleoplasmic pool of BR particles after 2 h of labelling. It is concluded that within the nucleus the BR particles move randomly. Furthermore, estimates of minimum diffusion coefficients for the BR particles are compatible with the view that the particles diffuse freely in the interchromosomal space, although it is not excluded that the random movement could be slightly retarded. Once the particles get bound to the nuclear pore complexes, they seem committed to translocation through the nuclear pores.
Singh
O P
OP
Medical Nobel Institute, Karolinska Institutet, Stockholm, SE-17177, Sweden.
Björkroth
B
B
Masich
S
S
Wieslander
L
L
Daneholt
B
B
D000818:Animals; D001692:Biological Transport; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002683:Chironomidae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D002875:Chromosomes / Q000648:ultrastructure; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D004058:Diffusion; D017344:Genes, Insect / Q000235:genetics; D007700:Kinetics; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012322:RNA Precursors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D012322:RNA Precursors; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein