[T lymphocyte immunological response to different antigenic fractions of Mycobacterium bovis in juvenile chronic polyarthritis].
BACKGROUND
The possible relationship of stress or heat-shock proteins (hsp) with the pathogenesis of autoimmune disease has been intensely studied recently. In adult rheumatoid arthritis, a bacterial hsp (65 kDa hsp from Mycobacterium tuberculosis or bovis) would have a cross reactivity with a hsp of ARTICULAR cartilage.


OBJECTIVE
To assess the cellular immune response to the 65 kA hsp from M Bovis in children.


METHODS
The proliferative response of peripheral mononuclear cells of 20 children with juvenile chronic polyarthritis and 20 healthy controls, against the 65 kDa hsp and other antigenic fractions from M bovis, was studied.


RESULTS
Patients with juvenile chronic polyarthritis had a intense reaction against 65 kDa fraction and a second fraction located between 32.5 and 27.5 kDa. Patients with a prolonged evolution of the disease (more than five years), reacted preferentially to an antigenic segment located between 32.5 and 27.5 kDa and those with a shorter evolution did so with an antigen of 27.5-18.5 kDa.


CONCLUSIONS
These results support the hypothesis that 65 kDa hsp from M bovis is involved in the pathogenesis of chronic juvenile polyarthritis and suggest that patients with short or prolonged evolutions of the disease would react to different antigenic fractions.
González
B
B
Unidad de Immunología, Hospital Luis Calvo Mackenna, Universidad de Chile, Santiago de Chile.
King
A
A
González
J
J
Bustos
C
C
Vergara
U
U
D000293:Adolescent; D017668:Age of Onset; D000942:Antigens, Bacterial / Q000032:analysis*; D001171:Arthritis, Juvenile / Q000276:immunology*; D002648:Child; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D009163:Mycobacterium bovis / Q000472:pathogenicity; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D006360:Heat-Shock Proteins
Respuesta immunológica de linfocitos T frente a diferentes fracciones antigénicas de M Bovis en poliartritis crónica juvenil.