[Comparative study on biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloidin in vitro culture].
OBJECTIVE
This study was to investigate the in vitro cultivation, biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloid, to elucidate the value of their application.


METHODS
A comparative study was performed on cell proliferation, cell morphology, cytogenetic feature and cell ultrastructure of fibroblasts from normal skin, hypertrophic scar and keloid by means of the technique of in vitro culture.


RESULTS
The results indicated that the fibroblasts isolated from normal skin, hypertrophic scar and keloid exhibited similar morphology and growth rates. The cytogenetic feature and cell ultrastructure were also alike.


CONCLUSIONS
According to our study and that of others, we conclude that it is entirely feasible to establish an in vitro culture model of fibroblasts from normal skin for investigation, prevention and treatment of scars.
Song
W
W
Plastic Surgery Hospital of Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Sun
G
G
Guan
Z
Z
D000293:Adolescent; D000328:Adult; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D007223:Infant; D007627:Keloid / Q000473:pathology*; D008875:Middle Aged; D012867:Skin / Q000648:ultrastructure*