Simultaneous determination of pyridoxine hydrochloride and doxylamine succinate from tablets by ion pair reversed-phase high-performance liquid chromatography (RP-HPLC).
A new, simple, precise, and rapid ion pair reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous determination of pyridoxine hydrochloride (PYR) and doxylamine succinate (DOX) in tablets. The stationary phase was a Microbondapak C18 column (10 mu, 300 mm x 3.9 mm i.d.). The mobile phase was water:methanol (60:40) containing 10 mM heptanesulfonic acid and 0.25% triethylamine and adjusted to pH 2.2 with orthophosphoric acid. Detection was carried out at 263 nm using an ultraviolet (UV) detector. The flow rate was 1.0 ml/min, and retention times were 3.65 min and 7.32 min for PYR and DOX, respectively. The linearity was obtained in the concentration range 0.5-500 micrograms/ml for PYR and DOX. Mean percentage recoveries were 100.20% and 101.20% for PYR and DOX, respectively.
Argekar
A P
AP
Department of Chemistry, Institute of Science, Mumbai, India.
Sawant
J G
JG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004319:Doxylamine / Q000031:analogs & derivatives* / Q000032:analysis; D004355:Drug Stability; D006634:Histamine H1 Antagonists / Q000032:analysis*; D016014:Linear Models; D011736:Pyridoxine / Q000032:analysis*; D015203:Reproducibility of Results; D013607:Tablets
D006634:Histamine H1 Antagonists; D013607:Tablets; D004319:Doxylamine; D011736:Pyridoxine; C035385:doxylamine succinate