title: Neocortical projections regulate the neostriatal proenkephalin gene expression.
abstract: There is controversial evidence that neocortical projections to the neostriatum may regulate the neostriatal expression of the proenkephalin (PEnk) gene. Therefore, we have studied PEnk gene expression in organotypic neocortico-neostriatal co-cultures as well as cultures of isolated neostriata. PEnk mRNA was determined with in situ hybridization. Removal of the neocortex caused a time-dependent reduction in the number of neostriatal cells which showed expression of the PEnk gene. A maximal decrease was seen after 3 days. Within 2 days after blockade of glutamate receptors of the NMDA type significantly fewer neostriatal cells expressed the PEnk gene, indicating that NMDA receptors mediated the expression of the gene. In isolated neostriatal slices in which the expression of the PEnk gene had been down-regulated, NMDA increased the number of cells which expressed the gene in a time-dependent manner. Maximal expression was observed after 3 days. This induction was reduced by nimodipine, which blocks L-type Ca2(+)-channels. The slow increase in PEnk gene expression caused by NMDA resembled a recruiting process. It seems to be specific for the neostriatum and may be due to the latter's neuronal organization.
authors.affiliation: Department of Pharmacology, Albert-Ludwigs-University, Freiburg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Just
authors.forename: C
authors.initials: C
authors.lastname: Olenik
authors.forename: D K
authors.initials: DK
authors.lastname: Meyer
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology; D004745:Enkephalins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D018698:Glutamic Acid / Q000502:physiology; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives; D019579:Neocortex / Q000502:physiology*; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism*; D009412:Nerve Fibers / Q000502:physiology; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology
chemical_list: D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; C029992:proenkephalin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D018698:Glutamic Acid; C013411:biocytin; D008239:Lysine; D002118:Calcium