Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector.
Tomato bushy stunt virus (TBSV) cDNA, positioned between a modified cauliflower mosaic virus 35S promoter and the hepatitis delta virus antigenomic ribozyme with a downstream nopaline synthase gene polyadenylation signal, established infections upon rub-inoculation of plants with intact plasmids. Application of this methodology produced a TBSV DNA-based gene vector which yielded readily detectable levels of localized foreign gene expression in inoculated leaves. This is the first demonstration of an infectious DNA from a member of the Tombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-inoculation of miniprep DNA onto a variety of plant species.
Scholthof
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Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843, USA. herscho@acs.tamu.edu
D001483:Base Sequence; D004279:DNA, Viral; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D018532:Pisum sativum; D010942:Plant Viruses; D010947:Plants, Toxic; D010957:Plasmids; D012328:RNA Viruses; D016337:RNA, Catalytic; D018724:Spinacia oleracea; D013997:Time Factors; D014026:Nicotiana; D017864:Tombusvirus
D004279:DNA, Viral; D016337:RNA, Catalytic; D005966:Glucuronidase