Phospholipase D and membrane traffic. Potential roles in regulated exocytosis, membrane delivery and vesicle budding.
It is now well-established that phospholipase D is transiently stimulated upon activation by G-protein-coupled and receptor tyrosine kinase cell surface receptors in mammalian cells. Over the last 5 years, a tremendous effort has gone to identify the major intracellular regulators of mammalian phospholipase D and to the cloning of two mammalian phospholipase D enzymes (phospholipase D1 and D2). In this chapter, we review the physiological function of mammalian phospholipase D1 that is synergistically stimulated by ADP ribosylation factor, Rho and protein kinase Calpha. We discuss the function of this enzyme in membrane traffic, emphasising the possible integrated relationships between consumption of vesicles in regulated exocytosis, membrane delivery and constitutive membrane traffic.
Jones
D
D
Department of Physiology, Rockefeller Building, University College London, University St., London WC1E 6JJ, UK.
Morgan
C
C
Cockcroft
S
S
D020727:ADP-Ribosylation Factors; D002462:Cell Membrane / Q000378:metabolism*; D018699:Coated Vesicles / Q000378:metabolism; D020756:Coatomer Protein; D005089:Exocytosis; D019204:GTP-Binding Proteins / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D008407:Mast Cells / Q000201:enzymology*; D008565:Membrane Proteins / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology*; D010739:Phospholipase D / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
D020756:Coatomer Protein; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D010739:Phospholipase D; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; D020742:rhoA GTP-Binding Protein