Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro.
BACKGROUND
Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system.


METHODS
Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA.


RESULTS
After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus.


CONCLUSIONS
This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.
Aoki
K
K
Howard Hughes Medical Institute, University of Michigan Medical Center, Department of Internal Medicine and Biological Chemistry, Ann Arbor 48109-0650, USA.
Barker
C
C
Danthinne
X
X
Imperiale
M J
MJ
Nabel
G J
GJ
D000256:Adenoviridae / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D002478:Cells, Cultured; D003360:Cosmids; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D019426:Integrases / Q000378:metabolism*; D007763:Lac Operon; D009130:Muscle, Smooth / Q000166:cytology; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D013552:Swine; D014162:Transfection; D014764:Viral Proteins
D004279:DNA, Viral; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases