Pyrimidine nucleotidases/phosphotransferases from human erythrocyte.
Two cytoplasmic pyrimidine 5'-nucleotidase have been purified from human erythrocytes to homogeneity and partially characterized. The two enzymes, indicated as PN-I and PN-II, preferentially hydrolyse pyrimidine 5'-monophosphates and 3'-monophosphates, respectively. The kinetic analysis demonstrate that pyrimidine 5'-nucleotidases, in the presence of suitable nucleoside substrates, can operate as phosphotransferases by transferring phosphate to various nucleoside acceptors, including nucleoside analogues known as important drugs widely used in chemotherapy.
Amici
A
A
Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Italy.
Emanuelli
M
M
Raffaelli
N
N
Ruggieri
S
S
Magni
G
G
D015720:5'-Nucleotidase / Q000097:blood*; D002384:Catalysis; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D010766:Phosphorylation; D010770:Phosphotransferases / Q000097:blood*
D010770:Phosphotransferases; D015720:5'-Nucleotidase