Inhibition of cAMP-mediated decidualization in human endometrial stromal cells by IL-1beta and laminin.
Both IL-1 and laminin are reported to inhibit progesterone receptor-mediated decidualization signals in endometrial stromal cells, but the effects of these two inhibitors on cAMP-mediated decidualization signals remain unknown. Furthermore, the interaction between IL-1-stimulated and laminin-stimulated signals has not been analyzed yet. In this study, interactions between these two decidualization-inhibitory signals and their effects on 8-Br-cAMP-mediated decidualization in human endometrial stromal cells were investigated. Prolactin release from decidualized stromal cells was measured by enzyme immunoassay in order to quantitate in vitro decidualization. Both IL-1beta and laminin were found to inhibit 8-Br-cAMP-induced decidualization in vitro in human endometrial stromal cells. IL-1beta dose-dependently inhibited decidualization of stromal cells cultured on both laminin-coated and collagen-coated dishes, while laminin inhibited the decidualization of stromal cells cultured both with and without IL-1beta. These results indicate that IL-1beta and laminin additively and mutually inhibit cAMP-mediated decidualization signals, and that they may inhibit these signals through different mechanisms.
Mizuno
K
K
Department of Obstetrics and Gynecology, Osaka City University Medical School, Osaka, Japan.
Tanaka
T
T
Umesaki
N
N
Ogita
S
S
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000502:physiology*; D003656:Decidua / Q000502:physiology*; D015232:Dinoprostone / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D011388:Prolactin / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology*
D007375:Interleukin-1; D007797:Laminin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011388:Prolactin; D017258:Medroxyprogesterone Acetate; D000242:Cyclic AMP; D015232:Dinoprostone