Isolation and some properties of a novel killer toxin-like protein produced by Streptomyces sp. F-287.
A killer toxin-like protein was found in the culture supernatant of a strain isolated from soil. The strain was classified and designated as Streptomyces sp. F-287. The molecular weight of the purified killer toxin-like protein was estimated to be 9,500 by SDS-PAGE. The purified protein was heat stable (100 degrees C, 5 min), pH stable (pH 6.0-9.0, 60 degrees C, for 30 min), and had a relatively wide action spectra. The SKLP showed a cytocidal effect on both budding yeast, Saccharomyces cerevisiae W303 (IC50 = 15.6 micrograms/ml) and on fission yeast, Schizosaccharomyces pombe SP870 (IC50 = 20.0 micrograms/ml). The SKLP also caused morphological changes on some sensitive yeasts and filamentous fungi. These characteristics are apparently different from known killer toxins. These results suggest that this is a novel killer toxin-like protein from Streptomyces sp. strain F-287.
Hiraga
K
K
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Japan.
Hayashi
S
S
Kitazawa
M
M
Oda
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000935:Antifungal Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D001427:Bacterial Toxins; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D012568:Schizosaccharomyces / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*
D000596:Amino Acids; D000935:Antifungal Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; C121318:putative killer toxin-like protein, Streptomyces sp.