[Observation of cicatricial fibroblasts in culture and its biological properties].
In order to study the biological properties of fibroblasts isolated from different tissues. The fibroblasts from normal skin, hypertrophic scar and keloid were cultured, respectively, in vitro, and their morphologies and growth kinetics were compared. The results revealed that although fibroblasts in keloid were irregularly arranged, crisscross and overlapping with loss of polarization, there was no significant difference in the 3 groups so far the cellular morphology of fibroblast itself, cellular growth curve, cellular mitotic index, cloning efficiency and DNA content provided those cultures were in the same cellular density and culture conditions. It was concluded that fibroblasts isolated from culture of normal skin, hypertrophic scar and keloid in vitro showed no significant difference in morphology and growth kinetics, on the contrary, their biological behaviors were quite similar.
Huang
Y
Y
Department of Plastic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, P.R. China.
Ren
L
L
Qin
Y
Y
D000293:Adolescent; D000328:Adult; D002056:Burns / Q000150:complications*; D002455:Cell Division; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000209:etiology / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007627:Keloid / Q000473:pathology; D008297:Male; D008940:Mitotic Index
D004247:DNA