Dephosphorylation of specific proteins during induction of senescence in immortal human fibroblasts expressing thermolabile SV40 T antigen.
Particular protein kinase inhibitors block a senescence-like phenomenon in SVts8 cells induced by a shift up in temperature. We characterized cellular proteins with affinity chromatography using one such inhibitor as a ligand. Two proteins of 56 and 100 kDa were found to be dephosphorylated specifically, probably due to induction of a protein phosphatase activity(s).
Fujii
M
M
Division of Biochemistry, Kihara Institute for Biological Research, Yokohama, Japan.
Joguchi
A
A
Ogino
H
H
Nakabayashi
K
K
Ayusawa
D
D
D000375:Aging / Q000502:physiology*; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts; D006358:Hot Temperature; D006801:Humans; D008024:Ligands; D008970:Molecular Weight; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D004791:Enzyme Inhibitors; D008024:Ligands; D047428:Protein Kinase Inhibitors; D011506:Proteins