title: [Gene transfer efficiency enhancement by inactivated Coxsakie virus B1 in primary skeletal muscle cells].
abstract: OBJECTIVE
  To study the effect of inactivated Coxsackie virus B1(CVB1), which was considered to have high affinity to skeletal muscle cell, on gene transfer efficiency of reporter gene, pCDNA3-LacZ, in primary muscle cells.
  
  
  METHODS
  Viruses were purified by polyethylene sedimentation followed by sucrose supercentrifugation. Virus, transferrin-polylysine(TfpL) and LacZ were designed as different concentrations and groups, then positive cells contained beta-galactosidase were calculated as gene transfer efficiency.
  
  
  RESULTS
  Inactivated CVB1 made an enhancement of gene transfer efficiency from less than 1% in TfpL+LacZ group to 15-20 per cent in CVB1+TfpL+LacZ group.
  
  
  CONCLUSIONS
  Like adenovirus, inactivated CVB1 can also increase gene transfer efficiency in muscle cells, which provides experimental basis for constructing new vector targeting to muscle cells.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University of Medical Science, Guangzhou, 510080 P.R.China. gjchen@gzsums.edu.cn
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Fang
authors.forename: H
authors.initials: H
authors.lastname: Yan
mesh_terms: D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000235:genetics*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D018482:Muscle, Skeletal / Q000166:cytology / Q000821:virology*