A requirement for the POU transcription factor, Brn-2, in corticotropin-releasing hormone expression in a neuronal cell line.
The expression of the endogenous CRF gene was examined in the human neuroblastoma cell line, BE(2)-M17. In this cell line, treatment with retinoic acid induces CRF mRNA transcription. We examined the requirement for the POU transcription factor, Bm-2, for this response. We confirmed that Bm-2 is expressed in retinoic acid-induced BE(2)-M17 cells. Expression of antisense Bm-2 message aborted the retinoic acid-mediated induction of CRF transcription. However, overexpression of Bm-2 was not sufficient for CRF expression in the absence of retinoic acid. These experiments support the hypothesis that Bm-2 is an intermediary for retinoic acid-induced CRF expression.
Ramkumar
T
T
Division of Biology and Biomedical Sciences, Washington University Medical School, Saint Louis, Missouri 63110, USA.
Adler
S
S
D001665:Binding Sites; D015139:Blotting, Southern; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter; D006367:HeLa Cells; D018398:Homeodomain Proteins; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism*; D050809:POU Domain Factors; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D018398:Homeodomain Proteins; D050809:POU Domain Factors; D016372:RNA, Antisense; D012333:RNA, Messenger; D014157:Transcription Factors; C085076:transcription factor Brn-2; D014212:Tretinoin; D004247:DNA; D003346:Corticotropin-Releasing Hormone