[Isolation and purification of a membrane hyaluronate-binding protein from embryonic human brain].
A membrane hyaluronate-binding protein from cerebral cortex of human embryonic brain (22-24 weeks) was purified by affinity, ion exchange chromatographies and gel-filtration. While gel-filtration analysis the protein had Mm 250 kDa. Electrophoresis under reduction conditions in the presence of DS-Na revealed a major band with Mm 85 kDa and two minor binds with Mm 68 and 36 kDa. The isolated protein did not react with antibodies against known hyaluronate-binding and other proteins with similar mass. The results show that a new membrane hyaluronate-binding protein was isolated and purified from human embryonic brain.
Tkach
V L
VL
Dnipropetrovsk State University.
Ushakova
G A
GA
Lepekhin
E A
EA
D002540:Cerebral Cortex / Q000196:embryology / Q000378:metabolism*; D005326:Fetal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding
D005326:Fetal Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D006820:Hyaluronic Acid
Vydelenie i ochistka membrannogo gialuronatsviazyvaiushcego belka iz mozga émbrionov cheloveka.