Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes.
Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg+ and sIg enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg+ and sIg- enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes.
O'Dowd
A M
AM
Marine Laboratory, Aberdeen, Scotland, UK.
Ellis
A E
AE
Secombes
C J
CJ
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000276:immunology; D007668:Kidney / Q000166:cytology; D007962:Leukocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D020317:Salmo salar / Q000276:immunology*; D012995:Solubility
D000936:Antigen-Antibody Complex; D007136:Immunoglobulins