Structure of the soluble domain of cytochrome f from the cyanobacterium Phormidium laminosum.
Cytochrome f from the photosynthetic cytochrome b(6)f complex is unique among c-type cytochromes in its fold and heme ligation. The 1. 9-A crystal structure of the functional, extrinsic portion of cytochrome f from the thermophilic cyanobacterium Phormidium laminosum demonstrates that an unusual buried chain of five water molecules is remarkably conserved throughout the biological range of cytochrome f from cyanobacteria to plants [Martinez et al. (1994) Structure 2, 95-105]. Structure and sequence conservation of the cytochrome f extrinsic portion is concentrated at the heme, in the buried water chain, and in the vicinity of the transmembrane helix anchor. The electrostatic surface potential is variable, so that the surface of P. laminosum cytochrome f is much more acidic than that from turnip. Cytochrome f is unrelated to cytochrome c(1), its functional analogue in the mitochondrial respiratory cytochrome bc(1) complex, although other components of the b(6)f and bc(1) complexes are homologous. Identical function of the two complexes is inferred for events taking place at sites of strong sequence conservation. Conserved sites throughout the entire cytochrome b(6)f/bc(1) family include the cluster-binding domain of the Rieske protein and the heme b and quinone-binding sites on the electrochemically positive side of the membrane within the b cytochrome, but not the putative quinone-binding site on the electrochemically negative side.
Carrell
C J
CJ
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Schlarb
B G
BG
Bendall
D S
DS
Howe
C J
CJ
Cramer
W A
WA
Smith
J L
JL
D001426:Bacterial Proteins / Q000737:chemistry; D001937:Brassica / Q000201:enzymology; D003198:Computer Simulation; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D000458:Cyanobacteria / Q000201:enzymology*; D003573:Cytochrome b Group / Q000737:chemistry; D045346:Cytochrome b6f Complex; D003580:Cytochromes / Q000737:chemistry*; D045348:Cytochromes f; D014450:Electron Transport Complex III / Q000737:chemistry; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012995:Solubility; D055672:Static Electricity; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003580:Cytochromes; D010940:Plant Proteins; D014867:Water; D045346:Cytochrome b6f Complex; D045348:Cytochromes f; D014450:Electron Transport Complex III