Na(+)-dependent and Na(+)-independent transport of L-arginine and L-alanine across dog intestinal brush border membrane vesicles.
We prepared intestinal brush border membrane vesicles (BBMVs) from beagle dogs fed a commercial diet (protein content: 24-26%), and investigated the characteristics of transport for basic and neutral amino acids across the intestinal BBMVs. To determined the kinetic parameters for L-arginine and L-alanine uptake, their total uptake was resolved into three routes: (1) Na(+)-dependent carrier-mediated transport; (2) Na(+)-independent carrier-mediated transport; and (3) simple diffusion. We could observe subtle, but clear-cut, Na(+)-dependent basic amino acid transport for the first time among studies with intestinal BBMVs prepared from mammals fed a normal diet. The Na(+)-dependent system for L-arginine transport can be best characterized as 'low affinity, low capacity', in contrast to that for L-alanine transport, which is 'low affinity, high capacity'. Maximal velocities of the Na(+)-dependent carrier-mediated transport are estimated to be higher for both L-arginine and L-alanine in dog intestinal BBMVs than in rabbit intestinal BBMVs reported previously. These results suggest that food habit of mammals is an important factor to decide the characteristic of system B0,+, a Na(+)-dependent carrier-mediated transport system common to basic and neutral amino acids across intestinal brush border membranes, as is protein content of the diet.
Hatanaka
T
T
Fuji Gotemba Research Laboratory, Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan. hatanakatkh@gt.chugai-pharm.co.jp
Nabuchi
Y
Y
Ushio
H
H
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport; D004032:Diet; D004044:Dietary Proteins / Q000378:metabolism; D004058:Diffusion; D004285:Dogs; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology*
D004044:Dietary Proteins; D001120:Arginine; D012964:Sodium; D008239:Lysine; D000409:Alanine