Calcium phosphate stones produced by Madin-Darby canine kidney (MDCK) cells inoculated in nude mice.
The canine renal distal tubular cell line Madin-Darby canine kidney (MDCK) forms calcium phosphate microliths during a long-term culture in vitro. We identified osteopontin (OPN) and calprotectin (CPT) from a urinary stone matrix. We recently also detected the expression of OPN and CPT in MDCK cells. The relationship between the mechanism of the stone formation and these stone matrix proteins is not yet known. Here, MDCK cells were cultured and inoculated in the subcutis of nude mice. After 4, 8 and 12 weeks, the inoculated tissues were resected, fixed and immunostained with polyclonal anti-human OPN and polyclonal anti-human CPT antibodies. Some serial specimens were stained with von Kossa's procedure. MDCK cells formed some follicular formations in the subcutis of nude mice at least at 12 weeks after transplantation. At 8 weeks after the inoculation, we detected small calcium phosphate stones with MDCK cells trapped in the follicles. The cells forming the stones also expressed both OPN and CPT. The CPT expression sites coincided with the stone formation sites. We confirmed that MDCK cells inoculated in nude mice had stone-forming potential, and we speculate that OPN and CPT play important roles in stone formation by MDCK cells.
Sakakura
T
T
Department of Urology and The 1st Department of Anatomy, Nagoya City University Medical School, Nagoya, Japan.
Fujita
K
K
Yasui
T
T
Sasaki
S
S
Mabuchi
Y
Y
Iguchi
M
M
Kohri
K
K
D000818:Animals; D002130:Calcium Phosphates / Q000378:metabolism*; D002460:Cell Line; D017690:Cell Transplantation; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006085:Graft Survival; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000378:metabolism; D014545:Urinary Calculi / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology
D002130:Calcium Phosphates; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D053495:Osteopontin; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous