Immune proteins and their gene expression in the silkworm, Bombyx mori.
Several self-defense proteins have been isolated from the silkworm, Bombyx mori and their amino acid sequences determined. These proteins include novel antibacterial proteins designated lebocin and moricin, and a novel lectin designated hemocytin, an insect homologue of mammalian von Willebrand factor. Antibacterial mechanisms of lebocin and moricin have been analyzed and their ability to form ion channels in bacterial membranes play an important role in defense against bacterial infection. cDNAs and genes encoding these proteins have been cloned to examine their induction mechanisms upon bacterial infection. Regulatory motifs such as the kappaB-like and GATA sequence have been identified in the B. mori antibacterial proteins. On the other hand, hemocytin gene expression was confirmed to occur upon bacterial infection and before pupation under naive conditions, suggesting that hemocytin plays an important role in both immunity and metamorphosis. Moreover, this review also describes the releasing mechanisms of a bacterial cell wall component, lipopolysaccharide (LPS), from intact bacteria, clearance of LPS from B. mori hemolymph and a possible signal transduction pathway for antibacterial protein gene expression.
Yamakawa
M
M
Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Ibaraki, Japan. yamakawa@nises.affrc.go.jp
Tanaka
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides; D012831:Bombyx / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D017344:Genes, Insect; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D037102:Lectins / Q000502:physiology; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D019476:Insect Proteins; D037102:Lectins; D008070:Lipopolysaccharides; D010455:Peptides; C092374:hemocytin protein, Bombyx mori; C095582:lebocin protein, Bombyx mori; C097209:moricin protein, Bombyx mori