title: Intraperitoneal transplantation of isologous mesothelial cells for prevention of adhesions.
abstract: OBJECTIVE
  To study the effect of transplantation of mesothelial cells on the formation of adhesions after peritoneal abrasion.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  Teaching hospital, Germany.
  
  
  METHODS
  30 isologous eight-week old Lewis rats to allow for harvesting of the greater omentum from a separate group.
  
  
  METHODS
  The first group (n = 10) served as mesothelial cell donors. The other animals had laparotomy and induction of adhesions by standardised abrasion of the peritoneum. The trial group (n = 10) were given a suspension of 10(6) mesothelial cells/100 g body weight intraperitoneally and the control group (n = 10) an equal volume of culture medium. After 10 days the animals were killed.
  
  
  METHODS
  Measurements of the areas of adhesions by computer aided morphometry.
  
  
  RESULTS
  The trial group developed a mean (SD) adhesion area of 122.7 (176.7) mm2, and the controls 310.5 (179.1) mm2. The corresponding medians (range) were 51.2 (0-547.1) and 274.3 (100.6-575.4). Transplantation of mesothelial cells resulted in a significant reduction in adhesion formation (Wilcoxon test, p<0.01).
  
  
  CONCLUSIONS
  Intraperitoneal transplantation of mesothelial cells is an effective way of reducing the formation of adhesions.
authors.affiliation: Department of Surgery, Medical Faculty, Rhenish-Westphalian Technical University, Aachen, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Bertram
authors.forename: L
authors.initials: L
authors.lastname: Tietze
authors.forename: M
authors.initials: M
authors.lastname: Hoopmann
authors.forename: K H
authors.initials: KH
authors.lastname: Treutner
authors.forename: C
authors.initials: C
authors.lastname: Mittermayer
authors.forename: V
authors.initials: V
authors.lastname: Schumpelick
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000637:transplantation*; D004848:Epithelium / Q000637:transplantation; D008297:Male; D009852:Omentum; D010532:Peritoneal Diseases / Q000517:prevention & control*; D010537:Peritoneum / Q000601:surgery*; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D000267:Tissue Adhesions / Q000517:prevention & control; D014185:Transplantation, Isogeneic; D014945:Wound Healing