Existence of uncoupling protein-2 antigen in isolated mitochondria from various tissues.
Antibodies against Escherichia coli-expressed uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs. The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein-1 (UCP1) isolated from brown adipose tissue. Moreover, anti-UCP2 and cross-reacting anti-UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated.
Jezek
P
P
Department of Membrane Transport Biophysics, Institute of Physiology, Prague, Czech Republic. jezek@sun1.biomed.cas.cz
Zácková
M
M
Reháková
Z
Z
Růzicka
M
M
Borecký
J
J
Skobisová
E
E
Brucknerová
J
J
Garlid
K D
KD
Gimeno
R E
RE
Tartaglia
L A
LA
D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism*; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D006224:Cricetinae; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D006801:Humans; D007473:Ion Channels; D026901:Membrane Transport Proteins; D008929:Mitochondria, Heart / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D024101:Mitochondrial Proteins; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D011994:Recombinant Proteins; C000604389:UCP2 protein, human; C000604392:UCP3 protein, human; C000604391:Ucp2 protein, rat; C000604394:Ucp3 protein, rat; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3