title: The complete primary structure of mouse 20S proteasomes.
abstract: The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3 (C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE.
authors.affiliation: Howard Hughes Medical Institute, and Departments of Molecular Genetics and Cell Biology, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, OH 45267-0524, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Elenich
authors.forename: D
authors.initials: D
authors.lastname: Nandi
authors.forename: A E
authors.initials: AE
authors.lastname: Kent
authors.forename: T S
authors.initials: TS
authors.lastname: McCluskey
authors.forename: M
authors.initials: M
authors.lastname: Cruz
authors.forename: M N
authors.initials: MN
authors.lastname: Iyer
authors.forename: E C
authors.initials: EC
authors.lastname: Woodward
authors.forename: C W
authors.initials: CW
authors.lastname: Conn
authors.forename: A L
authors.initials: AL
authors.lastname: Ochoa
authors.forename: D B
authors.initials: DB
authors.lastname: Ginsburg
authors.forename: J J
authors.initials: JJ
authors.lastname: Monaco
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C478802:Psmb5 protein, mouse; C478803:Psmb5 protein, rat