[Antiviral activity of poly(A)-poly(U) duplexes modified with cis-diammine-dichloroplatinum (II)].
Polyribonucleotide duplex poly(A).poly(U) was modified with cis-diammine dichloroplatinum (II) (cis-DDP). It was shown that the antiinfluenza protective activity of the modified duplex in mice increased with the degree of modification (rb) rising up to 0.2. The effect was different from that for poly(I).poly(C) and poly(G).poly(C). The interferon titers in the murine brain increased in parallel with increasing of the antiviral activity. It was assumed that the structural specificity of the poly(A).poly(U) duplex was responsible for the phenomenon and that cis-DDP interaction with N(7) atoms of the adenine heterocycles blocked the "abnormal" Hoogsteen pairing of adenines with uracils. As a result the antiviral activity increased because of lowering the quantity of the intramolecular defects and increasing the length of the regular double-stranded regions.
Aksenov
O A
OA
Research Institute of Children Diseases, Russian Academy of Medical Sciences, St. Petersburg.
Platonova
G A
GA
Timkovskiĭ
A L
AL
D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D002945:Cisplatin / Q000737:chemistry* / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D007372:Interferons / Q000627:therapeutic use; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D011063:Poly A-U / Q000737:chemistry* / Q000627:therapeutic use
D000998:Antiviral Agents; D011063:Poly A-U; D007372:Interferons; D002945:Cisplatin
Protivovirusnaia aktivnost' dupleksov poli(A)-poli(U), modifitsirovannykh tsis-diammin-dikhloplatinoĭ(II).