Diverse effects of neutrophil integrin occupation on respiratory burst activation.
Integrin occupation can alter the function of neutrophils (PMN), but the mechanism(s) involved is still unclear. This study demonstrated that the occupation of PMN integrins (especially those of the beta(3) subfamily) strongly enhances TNF stimulation of the respiratory burst but down-regulates that induced by PMA, fMLP, Con A, and serum treated zymosan. Treatment of PMN with genistein, staurosporine, and wortmannin, inhibitors of tyrosine kinases, protein kinase C, and phosphotidylinostol 3-kinase (PI 3-kinase) respectively, completely blocked the TNF-stimulated respiratory burst in PMN. Genistein and wortmannin enhanced the PMA-stimulated respiratory burst but only in cells adherent to RGD peptide. These findings suggest that PMN integrins (beta(3) subfamily) can generate signals that regulate the PMN agonist responses, probably through the activities of tyrosine kinases and PI 3-kinase.
Yan
S R
SR
Department of Periodontics, University of Pittsburgh School of Dental Medicine, Pittsburgh, Pennsylvania 15261, USA.
Novak
M J
MJ
D000906:Antibodies; D015703:Antigens, CD / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D039661:Integrin beta3; D016023:Integrins / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D011940:Receptor Aggregation / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000819:agonists / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015054:Zymosan / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000906:Antibodies; D015703:Antigens, CD; D005353:Fibronectins; D039661:Integrin beta3; D016023:Integrins; D009842:Oligopeptides; D010980:Platelet Membrane Glycoproteins; D047428:Protein Kinase Inhibitors; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D013481:Superoxides; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; C047981:arginyl-glycyl-aspartic acid; D015054:Zymosan; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate