title: Virtual instrumentation for pH measurements in biological systems.
abstract: In the present communication a personal computer control methodology for pH data acquisition and analysis in biological systems is reported. The instrumental control, acquisition, storage, processing and presentation of the experimental data are provided by a data acquisition board, a graphical programming software and numerical analysis/graphics software. The major objective of this work is to improve the performance and flexibility of the personal computer acquisition system compared with traditional approaches depending on potentiometric recorders. In particular, virtual instruments for interfacing pH meters of different brands to a personal computer and for measuring proton changes in lightly buffered solutions during enzymatic reactions are provided.
authors.affiliation: Dipartimento di Chimica Biologica, Università di Padova, Italy.
authors.forename: M P
authors.initials: MP
authors.lastname: Rigobello
authors.forename: F
authors.initials: F
authors.lastname: Cazzaro
authors.forename: G
authors.initials: G
authors.lastname: Scutari
authors.forename: A
authors.initials: A
authors.lastname: Bindoli
mesh_terms: D000818:Animals; D002021:Buffers; D003196:Computer Graphics; D001330:Electronic Data Processing; D006863:Hydrogen-Ion Concentration; D008838:Microcomputers; D011522:Protons; D051381:Rats; D012984:Software; D012996:Solutions
chemical_list: D002021:Buffers; D011522:Protons; D012996:Solutions