Determination of domperidone in human serum and human breast milk by high-performance liquid chromatography-electrospray mass spectrometry.
A sensitive and selective method for the determination of domperidone in human breast milk and serum has been developed. The same method may be successfully applied to both matrices to a lower limit of quantitation of 0.5 ng/ml. Samples are processed by a liquid-liquid extraction, and analyzed by LC-ESI-MS in positive ion mode. There was no interference, on the domperidone quantitation, from over 30 drugs. Samples from patients, at various times post-dose, were analyzed and a large number showed significant levels of domperidone in the breast milk as well as in the serum.
Zavitsanos
A P
AP
Hewlett-Packard (Canada) Ltd., Chemical Analysis Division, Mississauga, Ontario. paul_zavitsanos@hp.com
MacDonald
C
C
Bassoo
E
E
Gopaul
D
D
D000932:Antiemetics / Q000032:analysis* / Q000097:blood; D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000032:analysis* / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008895:Milk, Human / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000932:Antiemetics; D004294:Domperidone