Protein redesign.
It has been demonstrated that, for a number of proteins, it is possible to dramatically alter the connectivities between elements of secondary structure. Remarkably large loop insertions are tolerated and many redesigns have generated proteins that successfully fold to stable, active structures. Some redesigns have been entirely the choice of the investigators, whereas others have incorporated a randomization and selection step to identify optimal sequences. These studies have provided basic guidelines for the rational manipulation of protein structure and stability, they have allowed the dissection of folding pathways and they have generated proteins with the potential for practical therapeutic applications.
Regan
L
L
Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, New Haven, CT 06520, USA. lynne@csbgly.csb.yale.edu
D001426:Bacterial Proteins / Q000737:chemistry; D002826:Chorismate Mutase / Q000737:chemistry; D003459:Crystallins / Q000737:chemistry; D015195:Drug Design; D054477:Glutaredoxins; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry; D008958:Models, Molecular; D009124:Muscle Proteins / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010940:Plant Proteins; D011487:Protein Conformation; D011490:Protein Disulfide Reductase (Glutathione); D015202:Protein Engineering; D017510:Protein Folding; D016601:RNA-Binding Proteins / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D013329:Structure-Activity Relationship; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry; D038206:beta-Crystallin B Chain / Q000031:analogs & derivatives*; D018909:src Homology Domains
D001426:Bacterial Proteins; D003459:Crystallins; D054477:Glutaredoxins; D009124:Muscle Proteins; D010455:Peptides; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C054243:Rop protein, ColE1 plasmid; D038206:beta-Crystallin B Chain; C113634:beta-crystallin B2; C093336:chymotrypsin inhibitor 2; D016179:Granulocyte Colony-Stimulating Factor; D019809:Streptavidin; D010088:Oxidoreductases; D013762:Tetrahydrofolate Dehydrogenase; D011490:Protein Disulfide Reductase (Glutathione); D002826:Chorismate Mutase