Isolation and identification of a major urinary canthaxanthin metabolite in rats.
The urinary metabolic pattern after administration of the radiolabeled non-provitamin A carotenoid canthaxanthin was investigated in rats. In the rather complex HPLC urinary metabolic pattern a fraction was found which was conjugated. Deconjugation of the polar conjugates with glusulase, purification of the metabolite with HPLC and identification with GC-MS and NMR revealed that it was 3-hydroxy-4-oxo-7,8-dihydro-beta-ionone. This structure was confirmed by comparisons with HPLC retention times, UV/VIS- and NMR-spectroscopy and GC-MS of the synthesized compound.
Bausch
J
J
F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Liechti
H
H
Oesterhelt
G
G
Kistler
A
A
D000818:Animals; D016644:Canthaxanthin / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005505:Food Coloring Agents / Q000032:analysis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005505:Food Coloring Agents; D016644:Canthaxanthin