Beta-chemokines are produced in lungs of mice with mycoplasma respiratory disease.
The recruitment of mononuclear cells in lungs is a key event in the pathogenesis of mycoplasma respiratory disease, but the cascade of events responsible is unknown. Studies were conducted to determine whether beta-chemokines, which are chemotactic for lymphocytes and macrophages, are produced in murine mycoplasma respiratory disease. Our results show that mRNA expression of the macrophage chemoattractant factor (MCP-1) and macrophage inflammatory peptides (MIP-1alpha and MIP-1beta), but not RANTES, increases in Mycoplasma pulmonis-infected mice. Also, MCP-1 concentrations were much higher in lung extracts from mycoplasma-infected mice than in uninfected mice. As beta-chemokines are chemotactic for lymphocytes and macrophages, these results suggest that MCP-1, MIP-1alpha, and/or MIP-1beta, but not RANTES, contribute to mononuclear cell infiltration associated with murine mycoplasma respiratory disease. Thus, the activation of cells to produce beta-chemokines is associated with mycoplasma infection, and the beta-chemokines, along with other factors and cytokines, are most likely involved in the cascade of events leading to mycoplasma inflammatory disease.
Simecka
J W
JW
Department of Molecular Biology and Immunology, 3500 Camp Bowie Blvd. University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D004285:Dogs; D005260:Female; D008168:Lung / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D009174:Mycoplasma / Q000378:metabolism*; D009175:Mycoplasma Infections / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms
D018932:Chemokine CCL2; D018925:Chemokines; D012333:RNA, Messenger