Patterns and dynamics of SVZ cell migration in the postnatal forebrain: monitoring living progenitors in slice preparations.
Glial progenitors colonize the CNS widely in the perinatal period, but the pathways and mechanisms of migration are not well understood. We investigated the migration of progenitors from the neonatal rat forebrain subventricular zone (SVZ) by labeling them in vivo with a retrovirus encoding green fluorescent protein and visualizing movements by time lapse microscopy in slices. Cells within the dorsolateral SVZ moved in an undirected fashion but migrated radially and tangentially after emigration into white matter, cortex, and striatum. Cells in the striatal SVZ migrated parallel to the ventricular surface. During migration, elongation of the leading process and nuclear translocation were independent or linked. Orthogonal turning involved either cessation of cell body movement and formation of a new leading process or continuous cell body movement and bending of the leading process.
Kakita
A
A
Department of Pathology and The Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA. ak463@columbia.edu
Goldman
J E
JE
D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development; D003342:Corpus Striatum / Q000166:cytology / Q000254:growth & development; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D009457:Neuroglia / Q000166:cytology*; D009924:Organ Culture Techniques; D016548:Prosencephalon / Q000166:cytology* / Q000254:growth & development*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins