Accumulation of poly (3-hydroxybutyric acid) by some soil Streptomyces.
In a limited-scale survey, 55 soil streptomycetes were screened for the accumulation of poly (3-hydroxybutyrate) [PHB]. Only 18% of the isolates accumulated PHB ranging between 1.9-7.8% of the dry biomass. The promising isolate DBCC-719, identified as Streptomyces griseorubiginosus, accumulated PHB amounting to 9.5% of the mycelial dry mass in the early stationary phase when grown in chemically defined medium with 2% (wt/vol) glucose as the sole source of carbon. Nitrogen-limiting conditions were inhibitory to growth and PHB accumulation. The isolated polymer was highly soluble in chloroform, gave a sharp peak at 235 nm on digestion with concentrated H(2)SO(4), and had a characteristic infrared spectrum.
Manna
A
A
Microbiology Laboratory, Department of Botany, Calcutta University, Calcutta-700 019, India.
Banerjee
R
R
Paul
A K
AK
D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000378:metabolism*; D012988:Soil Microbiology; D013302:Streptomyces / Q000378:metabolism*
D003470:Culture Media; D011108:Polymers; D005947:Glucose; D020155:3-Hydroxybutyric Acid