A YAC-based physical map of the mouse genome.
A physical map of the mouse genome is an essential tool for both positional cloning and genomic sequencing in this key model system for biomedical research. Indeed, the construction of a mouse physical map with markers spaced at an average interval of 300 kb is one of the stated goals of the Human Genome Project. Here we report the results of a project at the Whitehead Institute/MIT Center for Genome Research to construct such a physical map of the mouse. We built the map by screening sequenced-tagged sites (STSs) against a large-insert yeast artificial chromosome (YAC) library and then integrating the STS-content information with a dense genetic map. The integrated map shows the location of 9,787 loci, providing landmarks with an average spacing of approximately 300 kb and affording YAC coverage of approximately 92% of the mouse genome. We also report the results of a project at the MRC UK Mouse Genome Centre targeted at chromosome X. The project produced a YAC-based map containing 619 loci (with 121 loci in common with the Whitehead map and 498 additional loci), providing especially dense coverage of this sex chromosome. The YAC-based physical map directly facilitates positional cloning of mouse mutations by providing ready access to most of the genome. More generally, use of this map in addition to a newly constructed radiation hybrid (RH) map provides a comprehensive framework for mouse genomic studies.
Nusbaum
C
C
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Slonim
D K
DK
Harris
K L
KL
Birren
B W
BW
Steen
R G
RG
Stein
L D
LD
Miller
J
J
Dietrich
W F
WF
Nahf
R
R
Wang
V
V
Merport
O
O
Castle
A B
AB
Husain
Z
Z
Farino
G
G
Gray
D
D
Anderson
M O
MO
Devine
R
R
Horton
L T
LT
Ye
W
W
Wu
X
X
Kouyoumjian
V
V
Zemsteva
I S
IS
Wu
Y
Y
Collymore
A J
AJ
Courtney
D F
DF
Tam
J
J
Cadman
M
M
Haynes
A R
AR
Heuston
C
C
Marsland
T
T
Southwell
A
A
Trickett
P
P
Strivens
M A
MA
Ross
M T
MT
Makalowski
W
W
Xu
Y
Y
Boguski
M S
MS
Carter
N P
NP
Denny
P
P
Brown
S D
SD
Hudson
T J
TJ
Lander
E S
ES
D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D020451:Contig Mapping; D005819:Genetic Markers; D016678:Genome; D051379:Mice / Q000235:genetics*; D008957:Models, Genetic; D020161:Physical Chromosome Mapping
D005819:Genetic Markers