Isoflavonoids do not inhibit in vivo lipid peroxidation in subjects with high-normal blood pressure.
The isoflavonoids genistein and daidzein have been shown to have antioxidant activity in vitro, but their effects on in vivo oxidation have not been assessed. The newly described F2-isoprostanes are believed to currently represent the best available marker of in vivo lipid peroxidation. Therefore we have assessed the effects of a 55 mg daily isoflavonoid supplement on urinary F2-isoprostane concentrations in subjects with high-normal blood pressure (BP). A total of 59 subjects completed an 8-week parallel design, randomized, double blind, and placebo-controlled study. F2-isoprostanes, isoflavonoids and creatinine were measured in 24-h urine samples taken at baseline and at the end of the intervention. There were significant increases in urinary excretion of genistein (5.22+/-0.75 mg/day, P < 0.0001) and daidzein (2.53+/-0.43 mg/day, P < 0.0001) in the group taking the isoflavonoid supplement. Creatinine excretion was significantly correlated with F2-isoprostanes at baseline (r = 0.45, P < 0.01). After adjustment for baseline values, there was no significant difference between groups in creatinine adjusted post-intervention F2-isoprostane concentrations (P = 0.74). In addition, changes in genistein and daidzein excretion were not significantly correlated with changes in F2-isoprostanes in the isoflavonoid treatment group. These results are not consistent with the suggestion that the two soy derived isoflavonoids have in vivo antioxidant activity at a level of intake achievable by dietary means and in subjects with high-normal BP.
Hodgson
J M
JM
University of Western Australia Department of Medicine and the Western Australian Heart Research Institute, Royal Perth Hospital, Australia. jonathan@cyllene.uwa.edu.au
Puddey
I B
IB
Croft
K D
KD
Mori
T A
TA
Rivera
J
J
Beilin
L J
LJ
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000493:pharmacokinetics / Q000494:pharmacology*; D001794:Blood Pressure; D003404:Creatinine / Q000652:urine; D015237:Dinoprost / Q000652:urine; D004311:Double-Blind Method; D005260:Female; D019833:Genistein / Q000494:pharmacology* / Q000652:urine; D006801:Humans; D006973:Hypertension / Q000378:metabolism*; D007529:Isoflavones / Q000494:pharmacology* / Q000652:urine; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged
D000975:Antioxidants; D007529:Isoflavones; C004742:daidzein; D003404:Creatinine; D015237:Dinoprost; D019833:Genistein