A putative role for carbohydrates in sea urchin gastrulation.
Many studies have examined the effects of lectins on embryonic development. Recently, it has been shown that lectins actually enter the blastocoel of sea urchin embryos without microinjection and bind to specific cell types. The present study was performed to examine the effects of lectins on sea urchin gastrulation. Strongylocentrotus purpuratus sea urchin embryos were incubated with several lectins at concentrations from 0.01 microgram/ml to 100 micrograms/ml at 15-28 h in the presence or absence of the preferential binding sugars. The most interesting findings were that the mannose specific lectins Lens culinaris agglutinin (LcH) which binds to secondary mesenchyme cells involved in archenteron anchoring and Pisum sativum (PSA) caused exogastrulation. Wheat germ agglutinin (WGA) which binds to primary mesenchyme cells involved in skeletogenesis caused defective skeletogenesis. Our findings suggest that D-mannose-like residues (LcH and PSA specific sugar) may function in archenteron development and anchoring, while N-acetyl-D-glucosamine-like groups (WGA specific sugar) may contribute to control of primary mesenchyme positioning and function. Specific carbohydrate-containing receptors may, therefore, be of importance in specific gastrulation events.
Latham
V H
VH
Department of Biology, California State University, Northridge 91330-8303, USA.
Tully
M J
MJ
Oppenheimer
S B
SB
D000818:Animals; D002241:Carbohydrates / Q000502:physiology*; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D005775:Gastrula / Q000187:drug effects / Q000502:physiology*; D037102:Lectins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008766:Methylmannosides / Q000494:pharmacology; D037121:Plant Lectins; D012617:Sea Urchins; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D002241:Carbohydrates; D037102:Lectins; D008766:Methylmannosides; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; C043696:jacalin; C033619:lentil lectin; C036292:pea lectin; D003208:Concanavalin A; C008466:methylmannoside