title: [Cloning of the idiopathic azoospermia related genes with mRNA differential display method].
abstract: OBJECTIVE
  Defect of some genes related to spermatogenesis may result in male infertility with azoospermia. This study aimed to clone some of the genes.
  
  
  METHODS
  Testis tissues from a patient with idiopathic azoospermia and from a normal fathered man were investigated with an improved mRNA differential display approach.
  
  
  RESULTS
  Significant difference was observed between the two tissues in gene expression. Five differential expressed sequence-tags (ESTs) were cloned and sequenced. Homology analysis with software advanced BLAST 2.0 showed that one EST shared 100% homology with cosmid L27h9, which located in Huntington's disease region on 4p16.3; the other 4 showed very low homology with sequences in GenBank.
  
  
  CONCLUSIONS
  Azoospermia has complex genetic heterogeneity. A gene located in Huntington's disease region on 4p16.3 is expressed in human testis during the spermatogenesis, and a loss of its function may associate with azoospermia.
authors.affiliation: Department of Medical Genetics, West China University of Medical Sciences, Chengdu, 610041 P.R.China. zszg@wcums.edu.cn
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Fan
authors.forename: Q
authors.initials: Q
authors.lastname: Xia
mesh_terms: D019976:Cloning, Organism; D006801:Humans; D006816:Huntington Disease / Q000235:genetics; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger