In vitro influence of parenteral lipid emulsions on the respiratory burst of neutrophils.
The in vitro effect of a fish oil-derived lipid emulsion (omega-3) on the superoxide anion production during the respiratory burst (RB) of human neutrophils was compared to a LCT lipid (Intralipid), and an LCT/MCT emulsion (Lipofundin MCT). The effects of two concentrations (60 and 600 micrograms/mL) were evaluated by rhodamine in a flow cytometer. The RB was induced either by stimulation with Escherichia coli (E. coli) or by priming with TNF-alpha and FMLP stimulation. The results (mean +/- SD%, P < 0.05) were compared to positive control responses (RB without lipids). omega-3 (60 micrograms/mL, -8.2 [9.3]%; 600 micrograms/mL, -9.6 [11.1]%) and LCT (600 micrograms/mL, -8.0 [9.3]%) significantly suppressed the RB after stimulation with E. coli. LCT/MCT increased the RB after E. coli (60 micrograms/mL, 15.7 [15.4]%; 600 micrograms/mL, 42.7 [21.4]%) as well as after TNF-alpha/FMLP stimulation (600 micrograms/mL, 27.4 [23.7]%). The in vitro influence of parenteral lipid emulsions on the superoxide anion production of human neutrophils is dependent on the length of the fatty acid molecule.
Heine
J
J
Department of Anesthesiology, Hannover Medical School, Germany. heine.joern@mh-hannover.de
Scheinichen
D
D
Jaeger
K
K
André
M
M
Leuwer
M
M
D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous / Q000009:adverse effects* / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010743:Phospholipids / Q000009:adverse effects / Q000737:chemistry; D016897:Respiratory Burst / Q000187:drug effects*; D013012:Sorbitol / Q000009:adverse effects / Q000737:chemistry; D013481:Superoxides / Q000378:metabolism
D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous; D010743:Phospholipids; D013481:Superoxides; C008288:Lipofundin; D013012:Sorbitol