The spheroplast lysis assay for yeast in microtiter plate format.
A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes.
Ovalle
R
R
Department of Biological Sciences and Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021, USA.
Spencer
M
M
Thiwanont
M
M
Lipke
P N
PN
D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D006867:Hydrolases / Q000494:pharmacology; D009172:Mycology / Q000379:methods* / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013104:Spheroplasts / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
C037833:zymolyase; D006867:Hydrolases