Pyrolysis mass spectrometry analysis of free-living and symbiotic cyanobacteria.
The potential of pyrolysis mass spectrometry to distinguish closely related cyanobacterial strains was assessed by using the technique to compare symbiotic cyanobacteria isolated from the hornwort Phaeoceros laevis and free-living cyanobacterial strains at the same field site. The same strains had previously been compared using polymerase chain reaction-based DNA fingerprinting techniques (West & Adams 1997, Appl. Environ. Microbiol. 63: 4479-4484). Many of the strains were grouped identically by the two techniques, although there were some differences, possibly resulting from the ability of these cyanobacteria to develop a range of specialised cell types having different chemical compositions to the vegetative cells. Although growth conditions were chosen to suppress cellular differentiation, this may not always have been completely successful. With careful control of growth conditions pyrolysis mass spectrometry has considerable potential as an additional tool for the phenetic comparison of cyanobacterial strains. It has the advantage that analysis is directly derived from whole cells, and hence is simpler and cheaper than DNA-based methods, although it does require the growth of axenic strains. The technique may be particularly useful in the study of some of the more cryptic unicellular and non-heterocystous filamentous cyanobacterial groups, in which the lack of cellular differentiation should minimise any variability in the chemical composition of cells.
West
N J
NJ
Department of Microbiology, School of Biochemistry and Molecular Biology, University of Leeds, UK.
Adams
D G
DG
Sisson
P R
PR
Freeman
R
R
Hawkey
P M
PM
D015373:Bacterial Typing Techniques; D000458:Cyanobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D013058:Mass Spectrometry / Q000379:methods; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology; D013559:Symbiosis