Structural organization of G-protein-coupled receptors.
Atomic-resolution structures of the transmembrane 7-alpha-helical domains of 26 G-protein-coupled receptors (GPCRs) (including opsins, cationic amine, melatonin, purine, chemokine, opioid, and glycoprotein hormone receptors and two related proteins, retinochrome and Duffy erythrocyte antigen) were calculated by distance geometry using interhelical hydrogen bonds formed by various proteins from the family and collectively applied as distance constraints, as described previously [Pogozheva et al., Biophys. J., 70 (1997) 1963]. The main structural features of the calculated GPCR models are described and illustrated by examples. Some of the features reflect physical interactions that are responsible for the structural stability of the transmembrane alpha-bundle: the formation of extensive networks of interhelical H-bonds and sulfur-aromatic clusters that are spatially organized as 'polarity gradients'; the close packing of side-chains throughout the transmembrane domain; and the formation of interhelical disulfide bonds in some receptors and a plausible Zn2+ binding center in retinochrome. Other features of the models are related to biological function and evolution of GPCRs: the formation of a common 'minicore' of 43 evolutionarily conserved residues; a multitude of correlated replacements throughout the transmembrane domain; an Na(+)-binding site in some receptors, and excellent complementarity of receptor binding pockets to many structurally dissimilar, conformationally constrained ligands, such as retinal, cyclic opioid peptides, and cationic amine ligands. The calculated models are in good agreement with numerous experimental data.
Lomize
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College of Pharmacy, University of Michigan, Ann Arbor 48109-1065, USA.
Pogozheva
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D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D017510:Protein Folding; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins