title: Effect of time post mortem on the concentration of endotoxin in rat organs: implications for sudden infant death syndrome (SIDS).
abstract: The aim of the study was to test the following hypotheses: (i) that endotoxin injected 40 min prior to death can be detected in rat organs post mortem and (ii) that endotoxin levels do not change with increasing time post mortem. Rats were injected with or without endotoxin in buffered saline, 40 min prior to being killed. Endotoxin levels in rat organs were assessed using a Limulus amoebocyte assay. The effect of storage time post mortem was assessed by following various storage regimes at 25 degrees C and 8 degrees C. Significant differences (P = < 0.001) in endotoxin levels of all samples tested were found between rats injected with and without endotoxin. A significant increase in detectable endotoxin was observed between 0 h and 6 h post mortem in rats injected with or without endotoxin. No difference in detectable endotoxin levels in the kidney, liver and spleen was observed from 30 h to 102 h post mortem in rats injected with or without endotoxin. In rats injected with endotoxin, detectable endotoxin levels in the heart were raised between 0 h and 6 h, 6 h and 54 h, and 30 h and 78 h. Endotoxin injected into rats 40 min prior to death can be detected post mortem. For rats injected with saline or endotoxin prior to death levels in the kidney, liver and spleen were not affected by storage at 8 degrees C for 30-102 h, after initial storage at room temperature for 6 h. Levels of endotoxin detected in the hearts of rats injected with saline were not affected by storage up to 102 h. In rats injected with endotoxin prior to death, detectable levels in the heart were significantly affected by increasing time in storage.
authors.affiliation: School of Biological Sciences, University of Manchester, UK.
authors.forename: N M
authors.initials: NM
authors.lastname: Sayers
authors.forename: B A
authors.initials: BA
authors.lastname: Crawley
authors.forename: K
authors.initials: K
authors.lastname: Humphries
authors.forename: D B
authors.initials: DB
authors.lastname: Drucker
authors.forename: B A
authors.initials: BA
authors.lastname: Oppenheim
authors.forename: L P
authors.initials: LP
authors.lastname: Hunt
authors.forename: J A
authors.initials: JA
authors.lastname: Morris
authors.forename: D R
authors.initials: DR
authors.lastname: Telford
mesh_terms: D000818:Animals; D004731:Endotoxins / Q000008:administration & dosage / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000737:chemistry; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000737:chemistry; D013398:Sudden Infant Death / Q000209:etiology; D013997:Time Factors
chemical_list: D004731:Endotoxins