Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
Using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) we studied the decomposition pathway of a prooligonucleotide during incubation in a crude cell extract. This study, involving no sample processing, except drop dailysis to remove salts, led us to identify more than thirty metabolites from several metabolic pathways.
Lioux
T
T
Laboratoire de Chimie Bioorganique, U.M.R. C.N.R.S. 5625, Case Courrier 008, Université Montpellier II, Place Eugéne Bataillon, 34095 Montpellier Cedex 5, France.
Lefebvre
I
I
Vasseur
J J
JJ
Imbach
J L
JL
D000818:Animals; D002460:Cell Line; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D009841:Oligonucleotides