title: An isolation procedure for arachidonic acid producing Mortierella species.
abstract: Malt extract agar and an incubation temperature of 5 degrees C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid.
authors.affiliation: Department of Microbiology and Biochemistry, University of the Orange Free State, South Africa. abo@maties.sun.ac.za
authors.forename: A
authors.initials: A
authors.lastname: Botha
authors.forename: I
authors.initials: I
authors.lastname: Paul
authors.forename: C
authors.initials: C
authors.lastname: Roux
authors.forename: J L
authors.initials: JL
authors.lastname: Kock
authors.forename: D J
authors.initials: DJ
authors.lastname: Coetzee
authors.forename: T
authors.initials: T
authors.lastname: Strauss
authors.forename: C
authors.initials: C
authors.lastname: Maree
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D003470:Culture Media; D020094:Mortierella / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D009172:Mycology / Q000379:methods; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D003470:Culture Media; D016718:Arachidonic Acid