title: Identification of a herpes simplex virus-induced dendrite in an eye-bank donor cornea.
abstract: OBJECTIVE
  To demonstrate the presence of an active herpetic dendrite in an eye-bank cornea.
  
  
  METHODS
  
  
  
  METHODS
  One eye-bank cornea was studied. Viral cultures and polymerase chain reaction (PCR) testing were performed 4 days after tissue preservation. The presence or absence of herpes simplex virus (HSV) type 1 was investigated.
  
  
  RESULTS
  The presence of an active HSV dendrite in an eye-bank cornea was verified. HSV type 1 was confirmed using PCR amplification and restriction endonuclease DNA fragment analysis.
  
  
  CONCLUSIONS
  This case suggests that HSV may remain viable in stored corneal tissue at 4 degrees C.
authors.affiliation: Department of Ophthalmology, University of Arkansas for Medical Sciences, Little Rock 72205-7199, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Neufeld
authors.forename: T L
authors.initials: TL
authors.lastname: Steinemann
authors.forename: L M
authors.initials: LM
authors.lastname: Merin
authors.forename: W G
authors.initials: WG
authors.lastname: Stroop
authors.forename: M F
authors.initials: MF
authors.lastname: Brown
mesh_terms: D003315:Cornea / Q000473:pathology* / Q000821:virology; D016039:Corneal Transplantation; D053938:DNA Fragmentation; D004279:DNA, Viral / Q000032:analysis; D005125:Eye Banks; D005500:Follow-Up Studies; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007635:Keratitis, Dendritic / Q000175:diagnosis* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014019:Tissue Donors
chemical_list: D004279:DNA, Viral