Simultaneous determination of 5-hydroxymethylfurfural and patulin in apple juice by reversed-phase liquid chromatography.
A rapid, simple and economical method was described for the simultaneous determination of 5-hydroxymethylfurfural (HMF) and patulin in apple juice. The sample was extracted with ethyl acetate and the extract was then cleaned up by extraction with a sodium carbonate solution. Then HMF and patulin were determined by reversed-phase liquid chromatography using a C18 column and a photodiode array detector. HMF and patulin could be completely resolved by using the mixture water-acetonitrile (99:1, v/v) as the mobile phase with a flow rate of 1.0 ml/min. Mean recoveries of HMF ranged from 86% to 100% with an overall mean of 94%, that of patulin ranged from 94% to 125% with an overall mean of 103%, for different spiking levels. The limits of detection for HMF and patulin in apple juice were found to be < 0.01 mg/l and < 5 micrograms/l, respectively.
Gökmen
V
V
Department of Food Engineering, Hacettepe University, Ankara, Turkey.
Acar
J
J
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D010365:Patulin / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
C008046:5-hydroxymethylfurfural; D010365:Patulin; D005662:Furaldehyde