Effects of glucagon on axoplasmic transport in mouse superior cervical ganglion cells.
The effects of glucagon on the axoplasmic transport of cultured mouse superior cervical ganglion cells were analyzed with the video-enhanced DIC microscope system. Glucagon increased the rate of fast axoplasmic transport by 30% in both anterograde and retrograde directions. The average velocity was increased from 1.36 +/- 0.48 microns/s to 1.74 +/- 0.43 microns/s (anterograde, n = 60) and from 1.37 +/- 0.48 microns/s to 1.62 +/- 0.39 microns/s (retrograde, n = 60). The stimulatory effect of glucagon on the axoplasmic transport was reversed in a glucose-free medium, whereas blocking the citrate cycle by pretreating neuronal cells with malonate did not alter the effect of glucagon. Together with our previous findings, our data suggest that neurotransmitters and hormones play a major role in the regulation of fast axoplasmic transport.
Tao
Y
Y
Department of Physiology, Yokohama City University School of Medicine, Japan.
Hori
H
H
Kawakami
T
T
Hashimoto
Y
Y
Takenaka
T
T
Ishikawa
Y
Y
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D003470:Culture Media / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D008314:Malonates / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D013997:Time Factors
D003470:Culture Media; D008314:Malonates; D005934:Glucagon; C030290:malonic acid; D005947:Glucose