Hypoxia-ischemia altered expression of glutamate transporter EAAT1 in neonatal rat brain.
OBJECTIVE
To observe altered expression of glutamate transporter EAAT1 after hypoxia-ischemia (H-I) in newborn rat brain.


METHODS
Expression levels of EAAT1 were detected with immunohistochemistry method.


RESULTS
EAAT1 was a little expressed in cerebral cortex at sham-operated group [(36 +/- 10) cells/slice]. Its expression in cerebral cortex increased at 24 h and 48 h following H-I [(314 +/- 162) cells/slice and (431 +/- 149) cells/slice, respectively], and recovered to control level at 72 h following H-I [(52 +/- 8) cells/slice]. The expression of EAAT1 in the ipsilateral cortex to common carotid artery (CCA) ligation was higher than that in the contralateral cortex.


CONCLUSIONS
After H-I, the expression of EAAT1 had a temporal change in cerebral cortex of newborn rat, and was mainly located in the ipsilateral cortex to CCA ligation.
Tao
F
F
Department of Neurobiology, Shanghai Medical University, China.
Zhang
L M
LM
Sun
F Y
FY
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG