Isochromosome 8q formation is associated with 8p loss of heterozygosity in a prostate cancer cell line.
BACKGROUND
In advanced prostate cancer, loss of chromosomal regions on 8p is frequently associated with gain of 8q. We studied the gross chromosomal abnormalities associated with 8p loss of heterozygosity (LOH) in the prostate tumor cell line 1542 CP3Tx. The cell line was previously established from a primary prostatic adenocarcinoma by immortalization with a recombinant retrovirus carrying the E6 and E7 genes of human papilloma virus type 16. Allelotyping studies demonstrated LOH at multiple markers on 8p.


METHODS
To investigate the relationship of 8p LOH to gross chromosomal rearrangements, and to screen for other genetic abnormalities in 1542 CP3Tx, we used comparative genomic hybridization (CGH), conventional karyotyping, fluorescence in situ hybridization (FISH), and allelotyping.


RESULTS
CGH revealed loss of the entire 8p arm, associated with gain of the entire 8q arm. Other abnormalities included chromosome 4 loss and chromosome 11 gain. The karyotype showed an isochromosome (8q), monosomy 4, and trisomy 11. FISH and allelotyping confirmed and extended these results.


CONCLUSIONS
These results demonstrate that i(8q) formation is a mechanism for associated 8p loss and 8q gain in prostate cancer. Furthermore, the small number of chromosomal abnormalities in this cell line indicates that immortalization of low-passage cultures with viral oncogenes provides a method for obtaining cell lines for studying genetic abnormalities in prostate cancer.
Virgin
J B
JB
Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, Michigan, USA. jvirgin@med.wayne.edu
Hurley
P M
PM
Nahhas
F A
FA
Bebchuk
K G
KG
Mohamed
A N
AN
Sakr
W A
WA
Bright
R K
RK
Cher
M L
ML
D000483:Alleles; D002871:Chromosome Banding; D020223:Chromosome Painting; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D005819:Genetic Markers