Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene.
Inactivation of the p53 tumor suppressor protein has been observed in a large number of human cancers. Overexpression of p53 induces either growth arrest or programmed cell death (apoptosis). The growth arrest function of p53 is mediated by induction of p21 (WAF1/CIP1), but the mechanisms underlying p53-dependent apoptosis are still largely unknown. To investigate these mechanisms, we have identified six differentially expressed transcripts in a human colon cancer cell line undergoing p53-dependent apoptosis. One of the p53-responsive genes showed significant homology to Drosophila peroxidasin, an extracellular matrix-associated peroxidase, and is likely to be its human homologue. Our results suggest a possible connection between p53-dependent apoptosis and the production of reactive oxygen species.
Horikoshi
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Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, 02115, USA. nhorikos@caregroup.harvard.edu
Cong
J
J
Kley
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N
Shenk
T
T
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D003110:Colonic Neoplasms / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D015870:Gene Expression; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D009195:Peroxidase / Q000235:genetics*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D017385:Sequence Homology; D014407:Tumor Cells, Cultured; D000097423:Peroxidasin
D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D009195:Peroxidase; D011506:Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000097423:Peroxidasin