Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing.
Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition motif preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs. We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing.
Mayeda
A
A
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33136, USA. amayeda@molbio.med.miami.edu
Badolato
J
J
Kobayashi
R
R
Zhang
M Q
MQ
Gardiner
E M
EM
Krainer
A R
AR
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012322:RNA Precursors; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012261:Ribonucleoproteins; D000068103:Serine-Arginine Splicing Factors; D018411:Spodoptera; D013347:Subcellular Fractions
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; C084538:RNPS1 protein, human; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; C498284:Rnps1 protein, mouse; D000068103:Serine-Arginine Splicing Factors