title: A nisin bioassay based on bioluminescence.
abstract: A Lactococcus lactis subsp. lactis strain that can sense the bacteriocin nisin and transduce the signal into bioluminescence was constructed. By using this strain, a bioassay based on bioluminescence was developed for quantification of nisin, for detection of nisin in milk, and for identification of nisin-producing strains. As little as 0.0125 ng of nisin per ml was detected within 3 h by this bioluminescence assay. This detection limit was lower than in previously described methods.
authors.affiliation: Institute of Biotechnology, FIN-00014 University of Helsinki, Finland.
authors.forename: G
authors.initials: G
authors.lastname: Wahlström
authors.forename: P E
authors.initials: PE
authors.lastname: Saris
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D001681:Biological Assay / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D008892:Milk / Q000737:chemistry; D009561:Nisin / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D005520:Food Preservatives; D009561:Nisin