[Preliminary study by flow cytometry of the cell cycle and DNA quantification in cytokeratin-positive cells in tumors of the head and neck].
DNA ploidy and cell-cycle distribution were determined by flow cytometry in fresh tumor tissue of 27 epithelial head and neck carcinomas. Epithelial cells were labeled with a fluorescein-isothiocyanate-conjugated cytokeratin antibody to study the possible influence of contaminating stromal and inflammatory cells on the results of cell-cycle analysis of tumor cells. The patient sample included 26 men and 1 women with a mean age of 60 years. Without cytokeratin gating, 11/27 tumors (40.74%) were aneuploid. After selecting the cytokeratin population, 10 more aneuploid tumors were found that had not been detected when considering the total population. Therefore, aneuploid tumors increased from 40.74% to 77.74%. The remaining 6/27 (22.26%) tumors were diploid. In the tumors that were either aneuploid without cytokeratin gating or diploid, the S-phase and G2M phase were significantly higher after cytokeratin staining, specially in diploid tumors (24.2% versus 10% and 6.8% versus 3.2%, respectively, p < 0.01). Therefore, in head and neck tumors cytokeratin staining optimizes both the determination of DNA ploidy and cell-cycle analysis, which is advantageous for tumor staging and prognosis assessment in these patients.
Górriz
C
C
Servicio de ORL, Clínica Puerta de Hierro, Madrid, 28035, España.
González
F M
FM
Castejón
R
R
García-Berrocal
J R
JR
Durántez
A
A
Vargas
J A
JA
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000235:genetics; D000912:Antibodies, Neoplasm; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D007399:Interphase / Q000235:genetics; D015603:Keratinocytes / Q000166:cytology; D007633:Keratins / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D019655:Quantitative Trait, Heritable
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D004273:DNA, Neoplasm; D007633:Keratins
Estudio preliminar del ciclo celular y cuantificación del ADN de células citokeratina positivas en tumores de cabeza y cuello mediante citometría de flujo.