title: Difference of acrosomal serine protease system between mouse and other rodent sperm.
abstract: A fraction of acrosomal proteins dispersed during calcium ionophore A23187-induced acrosome reaction was prepared from cauda epididymal sperm of wild-type and acrosin-deficient mice, rat, and hamster. The acrosome-reacted sperm were further extracted by Nonidet P-40 to obtain the detergent-soluble protein fraction. Activities of serine proteases in the two protein fractions were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of gelatin. A mixture of 42- and 41-kDa gelatin-hydrolyzing proteases was found in both fractions of the wild-type mouse sperm, whereas the acrosin-deficient mouse sperm contained the active 42-kDa protease and apparently lacked the activity of the 41-kDa protease. However, exogenous bovine pancreatic trypsin compensated for the absence of acrosin in the protein fractions of the mutant mouse sperm; the gelatin-hydrolyzing activity of the 41-kDa protease appeared when the sperm proteins of the mutant mice were treated with pancreatic trypsin. Two-dimensional polyacrylamide gel electrophoresis revealed that the 42- and 41-kDa proteases were distinguished from acrosin by the isoelectric point and immunoreactivity with affinity-purified antibody against an oligopeptide corresponding to the N-terminal amino acid sequence of mouse proacrosin. Moreover, the gelatin-hydrolyzing proteins corresponding to these two proteases were not detected in rat and hamster sperm, in spite of the treatment of the sperm extracts with pancreatic trypsin, and the total amount of gelatin-hydrolyzing activities in mouse was much smaller than those in rat and hamster. These results may reflect the difference of the serine protease system for the sperm penetration through the egg zona pellucida between mouse and other rodent animals, possibly explaining why the acrosin-deficient mouse sperm are capable of penetrating the zona pellucida.
authors.affiliation: Institute of Applied Biochemistry (IAB) and Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba Science City, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamagata
authors.forename: A
authors.initials: A
authors.lastname: Honda
authors.forename: S I
authors.initials: SI
authors.lastname: Kashiwabara
authors.forename: T
authors.initials: T
authors.lastname: Baba
mesh_terms: D000176:Acrosin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D000177:Acrosome / Q000201:enzymology*; D020101:Acrosome Reaction / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D006224:Cricetinae; D005260:Female; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D012697:Serine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D013084:Sperm-Ovum Interactions / Q000502:physiology
chemical_list: D007476:Ionophores; D000001:Calcimycin; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D012697:Serine Endopeptidases; D000176:Acrosin