title: Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24.
abstract: Rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from 124 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalenetype dioxygenase. DNA sequence analysis of a portion of this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase. These genes will be useful for manipulating indene bioconversion in Rhodococcus strain 124.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Treadway
authors.forename: K S
authors.initials: KS
authors.lastname: Yanagimachi
authors.forename: E
authors.initials: E
authors.lastname: Lankenau
authors.forename: P A
authors.initials: PA
authors.lastname: Lessard
authors.forename: G
authors.initials: G
authors.lastname: Stephanopoulos
authors.forename: A J
authors.initials: AJ
authors.lastname: Sinskey
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D005798:Genes, Bacterial; D007192:Indenes / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010957:Plasmids; D012240:Rhodococcus / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D007192:Indenes; D010088:Oxidoreductases; D010105:Oxygenases