title: Transfusion-associated graft-versus-host disease: an in situ hybridization analysis of the infiltrating donor-derived cells in the cutaneous lesion.
abstract: BACKGROUND
  Acute graft-versus-host disease (GVHD) can occur after a blood transfusion.
  
  
  OBJECTIVE
  In order to elucidate the pathomechanisms responsible for transfusion-associated GVHD, infiltrating donor-derived cells in a cutaneous lesion were analyzed.
  
  
  METHODS
  A skin sample obtained from a 69-year-old woman who developed fatal GVHD after blood transfusions from male donors was studied by performing in situ hybridization (ISH) with a Y-chromosome-specific probe.
  
  
  RESULTS
  The cell infiltrates comprised mainly CD3+ T lymphocytes. Immunohistochemistry and ISH in combination demonstrated that 99% (182/184) of the Y-body-positive cells were CD3+. Y bodies were observed in 80% of the CD8+ cells in the epidermis and dermoepidermal junction and in 77 and 45% of the CD8+ and CD4+ cells, respectively, in the dermis.
  
  
  CONCLUSIONS
  These findings suggest that both CD4+ and CD8+ cells of donor origin were involved in the development of cutaneous GVHD.
authors.affiliation: Department of Dermatology, School of Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Tanei
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: S
authors.initials: S
authors.lastname: Ishihara
authors.forename: K
authors.initials: K
authors.lastname: Katsuoka
authors.forename: H
authors.initials: H
authors.lastname: Yokono
authors.forename: T
authors.initials: T
authors.lastname: Motoori
mesh_terms: D000368:Aged; D001706:Biopsy; D015704:CD4 Antigens / Q000032:analysis; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000032:analysis; D015342:DNA Probes; D017809:Fatal Outcome; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D012867:Skin / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D065227:Transfusion Reaction; D014998:Y Chromosome / Q000235:genetics
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D015342:DNA Probes