Isolation and species typing of Lyme borreliosis spirochaetes from UK patients with erythema migrans.
Skin biopsies taken from UK cases of erythema migrans rash were cultured for Borrelia burgdorferi sensu lato. Reverse line blotting was used to type the infecting genospecies in PCR-positive cultures and biopsies. B. garinii or B. afzelii was identified in 56% (5/9) of biopsies/cultures tested. All patients were tested by conventional serology. PCR confirmed infection in two patients where serological testing failed to detect antibody.
Robertson
J
J
Lyme Disease Reference Unit, Public Health Laboratory, Southampton General Hospital, UK. jnr@soton.ac.uk
Murdoch
S
S
Foster
L
L
Green
S
S
D015373:Bacterial Typing Techniques; D001706:Biopsy; D015748:Borrelia burgdorferi Group / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D015787:Erythema Chronicum Migrans / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D008193:Lyme Disease / Q000175:diagnosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D006113:United Kingdom