title: JIL-1: a novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila.
abstract: We have cloned and characterized JIL-1, a novel tandem kinase in Drosophila that associates with the chromosomes throughout the cell cycle. Antibody staining and live imaging of JIL-1-GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost 2-fold on the hypertranscribed male X chromosome compared to autosomes. Phylogenetic analysis suggests that JIL-1 together with human MSKs defines a separate family of tandem kinases. That JIL-1 is a functional kinase was demonstrated by autophosphorylation and phosphorylation of histone H3 in vitro. Based on these findings, we propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure.
authors.affiliation: Department of Zoology and Genetics, Iowa State University, Ames 50011, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: D L
authors.initials: DL
authors.lastname: Walker
authors.forename: H
authors.initials: H
authors.lastname: Dong
authors.forename: C
authors.initials: C
authors.lastname: Conley
authors.forename: J
authors.initials: J
authors.lastname: Johansen
authors.forename: K M
authors.initials: KM
authors.lastname: Johansen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D002453:Cell Cycle; D002843:Chromatin / Q000737:chemistry; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004330:Drosophila / Q000196:embryology / Q000201:enzymology*; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D012469:Salivary Glands / Q000201:enzymology; D014960:X Chromosome / Q000235:genetics
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D019476:Insect Proteins; D011494:Protein Kinases