High affinity displacement of [(3)H]NPY binding to the crude venom of conus anemone by insect neuropeptides.
The venom from Conus anemone contains a protein, named ANPY toxin, which displayed high affinity (IC(50) in nanomolar range) to neuropeptide Y (NPY), [Leu(31), Pro(34)]NPY, peptide YY, pancreatic polypeptide, the Y(1) antagonist 1229U91, and C-terminal NPY fragments. N-terminal fragments and the free acid form of NPY did not bind to ANPY. The truncated NPY fragments displayed very low affinity to Y(1) receptors and partially inhibited [(3)H]NPY binding to anti-NPY antiserum. Several insect neuropeptides, the sequences of which related to the C-terminal fragments of NPY, were observed to bind with similar affinity or even 20 times higher (Lom-MS and Scg-NPF) affinity than NPY. In contrast, no significant binding of these insect peptides was observed for Y(1) receptors and anti-NPY antiserum. Therefore, ANPY can be viewed as an acceptor that binds with very high affinity to a broad spectrum of vertebrate and invertebrate neuropeptides that share a similar C-terminal amino acid sequence.
Le
M T
MT
Institute for Molecular Biology and Biotechnology, Free University of Brussels (VUB), Paardenstraat 65, Sint-Genesius Rode, B-1640, Belgium.
Vanderheyden
P M
PM
De Backer
J P
JP
Vanquelin
G
G
Broeck
J V
JV
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D033364:Decapoda; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D007313:Insecta; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000737:chemistry / Q000378:metabolism*; D009478:Neuropeptide Y / Q000031:analogs & derivatives / Q000276:immunology / Q000378:metabolism*; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D019894:Peptide YY / Q000378:metabolism; D011485:Protein Binding; D017476:Receptors, Neuropeptide Y / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D007106:Immune Sera; D019476:Insect Proteins; D008978:Mollusk Venoms; D009478:Neuropeptide Y; D009479:Neuropeptides; D010446:Peptide Fragments; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; D019894:Peptide YY; D010191:Pancreatic Polypeptide