title: Atypical mucocutaneous leishmaniasis caused by Leishmania braziliensis in an acquired immunodeficiency syndrome patient: T-cell responses and remission of lesions associated with antigen immunotherapy.
abstract: An atypical case of acquired immunodeficiency syndrome-associated mucocutaneous lesions due to Leishmania braziliensis is described. Many vacuolated macrophages laden with amastigote forms of the parasite were found in the lesions. Leishmanin skin test and serology for leishmaniasis were both negative. The patient was resistant to therapy with conventional drugs (antimonial and amphotericin B). Interestingly, remission of lesions was achieved after an alternative combined therapy of antimonial associated with immunotherapy (whole promastigote antigens). Peripheral blood mononuclear cells were separated and stimulated in vitro with Leishmania antigens to test the lymphoproliferative responses (LPR). Before the combined immunochemotherapy, the LPR to leishmanial antigens was negligible (stimulation index - SI=1.4). After the first course of combined therapy it became positive (SI=4.17). The antigen responding cells were predominantly T-cells (47.5%) most of them with CD8+ phenotype (33%). Very low CD4+ cells (2.2%) percentages were detected. The increased T-cell responsiveness to leishmanial antigens after combined therapy was accompanied by interferon-g (IFN-g) production as observed in the cell culture supernatants. In this patient, healing of the leishmaniasis lesions was associated with the induction of a specific T-cell immune response, characterized by the production of IFN-g and the predominance of the CD8+ phenotype among the Leishmania-reactive T-cells.
authors.affiliation: Laboratório de Imunidade Celular e Humoral, Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, 21045-900, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: Da-Cruz
authors.forename: D V
authors.initials: DV
authors.lastname: Filgueiras
authors.forename: Z
authors.initials: Z
authors.lastname: Coutinho
authors.forename: W
authors.initials: W
authors.lastname: Mayrink
authors.forename: G
authors.initials: G
authors.lastname: Grimaldi
authors.forename: P M
authors.initials: PM
authors.lastname: De Luca
authors.forename: S C
authors.initials: SC
authors.lastname: Mendonca
authors.forename: S G
authors.initials: SG
authors.lastname: Coutinho
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000276:immunology; D000818:Animals; D007111:Immunity, Cellular; D007167:Immunotherapy; D007892:Leishmania braziliensis; D007897:Leishmaniasis, Mucocutaneous / Q000276:immunology / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology*