Spo76p is a conserved chromosome morphogenesis protein that links the mitotic and meiotic programs.
Spo76p is conserved and related to the fungal proteins Pds5p and BIMD and the human AS3 prostate proliferative shutoff-associated protein. Spo76p localizes to mitotic and meiotic chromosomes, except at metaphase(s) and anaphase(s). During meiotic prophase, Spo76p assembles into strong lines in correlation with axial element formation. As inferred from spo76-1 mutant phenotypes, Spo76p is required for sister chromatid cohesiveness, chromosome axis morphogenesis, and chromatin condensation during critical transitions at mitotic prometaphase and meiotic midprophase. Spo76p is also required for meiotic interhomolog recombination, likely at postinitiation stage(s). We propose that a disruptive force coordinately promotes chromosomal axial compaction and destabilization of sister connections and that Spo76p restrains and channels the effects of this force into appropriate morphogenetic mitotic and meiotic outcomes.
van Heemst
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Institut de Génétique et Microbiologie, UMR 8621, Université Paris-Sud, Orsay, France.
James
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Pöggeler
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Berteaux-Lecellier
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Zickler
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D
D000595:Amino Acid Sequence; D000705:Anaphase / Q000502:physiology; D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins / Q000235:genetics*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D015825:Chromosomes, Fungal; D017124:Conserved Sequence; D003360:Cosmids; D004268:DNA-Binding Proteins / Q000235:genetics; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase / Q000502:physiology; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology; D011418:Prophase / Q000502:physiology; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D029701:Saccharomyces cerevisiae Proteins; D020037:Sordariales / Q000235:genetics*; D014157:Transcription Factors
D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; C513033:DMC1 protein, S cerevisiae; D004268:DNA-Binding Proteins; C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D005656:Fungal Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; C120540:Spo76 protein, Neurospora crassa; D014157:Transcription Factors; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; C074556:DMC1 protein, human