title: [Adsorption on synthetic membranes and immunogenic properties of aprotinin cross-linked by glutaraldehyde].
abstract: A procedure for the intermolecular crosslinking of aprotinin (natural polypeptide, an inhibitor of serine proteases) by glutaraldehyde was proposed. This autoconjugation of aprotinin increased its immunogenicity and the efficiency of sorption on nitrocellulose and polyvinylidene difluoride membranes. The immunization of animals by the autoconjugated aprotinin induced the production of antibodies that reacted with both conjugated and monomeric aprotinin. The properties of the crosslinked aprotinin are promising for the enhancement of sensitivity through its use in EIA and immunoblotting membrane methods that employ aprotinin and antiaprotinin antibodies.
authors.affiliation: Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Timofeeva
authors.forename: P B
authors.initials: PB
authors.lastname: Goliando
authors.forename: O P
authors.initials: OP
authors.lastname: Zhirnov
mesh_terms: D000327:Adsorption; D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D007611:Aprotinin / Q000737:chemistry* / Q000276:immunology; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry*; D008566:Membranes; D051379:Mice; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000276:immunology
chemical_list: D000941:Antigens; D003432:Cross-Linking Reagents; D015842:Serine Proteinase Inhibitors; D007611:Aprotinin; D005976:Glutaral
vernacular_title: Adsorbtsiia na sinteticheskikh membranakh i immunogennye svoĭstva aprotinina, kon''iugirovannogo s pomoshch'iu glutarovogo al'degida.