title: Enteral IGF-I enhances fetal growth and gastrointestinal development in oesophageal ligated fetal sheep.
abstract: Infants with upper gut atresia often have impaired intrauterine growth and gut function. IGF-I is important in fetal growth and is contained in amniotic fluid. We therefore wanted to test the hypothesis that IGF-I infused into fetal gut would reverse the effects of an upper gut obstruction on gut structure and growth in fetal sheep. At 90 days gestation fetuses (n=6 per group) underwent oesophageal ligation, followed by continuous infusion of IGF-I (1-8 microgram/day) or saline into the gut beyond the ligation until 137 days. Controls underwent sham ligation only. Oesophageal ligation tended to reduce fetal body and organ weights. IGF-I treatment prevented this reduction and increased body length and spleen weight above those of controls. The decrease in bowel wall thickness induced by oesophageal ligation was also prevented by IGF-I treatment. Amniotic fluid IGF-I concentrations did not change over gestation and were higher in the IGF-I treated group. No change in fetal plasma IGF-I concentrations were detectable. We conclude that enterally administered IGF-I may enhance fetal growth and gut development in utero and that IGF-I in amniotic fluid may play a physiological role in gut development in the fetus.
authors.affiliation: Research Centre for Developmental Medicine and Biology, Department of Paediatrics, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: R M
authors.initials: RM
authors.lastname: Kimble
authors.forename: B H
authors.initials: BH
authors.lastname: Breier
authors.forename: P D
authors.initials: PD
authors.lastname: Gluckman
authors.forename: J E
authors.initials: JE
authors.lastname: Harding
mesh_terms: D000818:Animals; D004064:Digestive System / Q000196:embryology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004947:Esophagus; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008026:Ligation; D009929:Organ Size; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D012756:Sheep
chemical_list: D007334:Insulin-Like Growth Factor I