title: Short exposure of polymorphonuclear leucocytes to sodium fluoride suppresses the response to fMLP.
abstract: Fluoridated dental care products are used to prevent dental decay. Up to now, there are no data available on whether the fluoride (F-) component of these products affects the bactericidal activity of salivary polymorpho-nuclear leucocytes, which are involved in the protection of the oral mucosa against infection. Therefore, after determining the concentration/time profile of F- in mixed saliva of healthy subjects after topical application of 0.5 g of a 1.25% F- containing gel, unstimulated and fMLP-stimulated polymorphonuclear leucocytes (PMNs) were shortly exposed to these F- concentrations and the generation of superoxide and hypochloric acid were measured, as well as the liberation of lysomal enzymes, and correlated with the cellular Ca2+ and cAMP-levels. The results show that F-, at concentrations as retained in saliva, did not activate the oxidative burst in unstimulated PMNs. In fMLP-activated PMNs, F-suppressed the receptor-mediated increase in the oxidative burst and the liberation of fl-glucuronidase by reduction of the availability of extracellular Ca2+ and, thus, the influx of Ca2+ necessary to couple completely the fMLP signal to effector pathways. These F- concentrations neither altered the liberation of Ca2+ from internal stores nor induced a rise in cAMP. The possible clinical consequences of these results for xerostomic patients with respect to the generation of HOSCN/OSCN/SCN in saliva an important non-immune factor for oral health, are dicussed.
authors.affiliation: Institute of Pharmacology, Medical Centre Benjamin Franklin Free University of Berlin, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Knoll-Köhler
authors.forename: G
authors.initials: G
authors.lastname: Büssem
mesh_terms: D000328:Adult; D002118:Calcium / Q000097:blood; D000242:Cyclic AMP / Q000097:blood; D005089:Exocytosis; D005260:Female; D006801:Humans; D006997:Hypochlorous Acid / Q000097:blood / Q000737:chemistry; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D016897:Respiratory Burst; D012463:Saliva / Q000737:chemistry; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood; D013654:Taurine / Q000737:chemistry
chemical_list: D013481:Superoxides; D013654:Taurine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006997:Hypochlorous Acid; D012969:Sodium Fluoride; D000242:Cyclic AMP; D002118:Calcium