title: Intraoperative embolus formation during cardiopulmonary bypass affects the release of S100B.
abstract: BACKGROUND
  Intraoperative thromboembolism and the systemic inflammatory reaction are thought to play a role in causing cerebral dysfunction following cardiopulmonary bypass (CPB). Increased levels of S100B, an astroglial protein, have been linked to neuropsychological deficits after CPB. The present study investigated whether S100B release correlates with intraoperative embolus formation, thrombin formation, or the release of inflammatory parameters.
  
  
  METHODS
  40 patients undergoing coronary artery bypass grafting were included. Blood samples were taken before, during, and after CPB, and levels of S100B, thrombin-antithrombin complex (TAT), complement C5a, and interleukin 8 were analysed. Embolus formation was assessed by Doppler ultrasound at the arterial line of CPB.
  
  
  RESULTS
  The release of S100B correlated with embolus count (r = 0.42; p = 0.009) and TAT formation (r = 0.71; p = 0.0001). The correlation of S100B with interleukin 8 (r = 0.58; p = 0.0001) was due to the dependence of both parameters on bypass time (r = 0.29; p = 0.075, partial correlation). A correlation of S100B with C5a formation could not be observed.
  
  
  CONCLUSIONS
  S100B release is related to embolus and thrombin formation during CPB, indicating that thrombofibrinous embolism is involved in perioperative brain damage. Inflammatory parameters (i.e. interleukin 8 and C5a) seem to have no influence on S100B release.
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital, Würzburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Babin-Ebell
authors.forename: M
authors.initials: M
authors.lastname: Misoph
authors.forename: W
authors.initials: W
authors.lastname: Müllges
authors.forename: K
authors.initials: K
authors.lastname: Neukam
authors.forename: J
authors.initials: J
authors.lastname: Reese
authors.forename: O
authors.initials: O
authors.lastname: Elert
mesh_terms: D001925:Brain Damage, Chronic / Q000097:blood / Q000175:diagnosis; D002135:Calcium-Binding Proteins / Q000097:blood*; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D005260:Female; D006801:Humans; D002542:Intracranial Embolism and Thrombosis / Q000097:blood / Q000175:diagnosis; D007431:Intraoperative Complications / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000097:blood*; D012307:Risk Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000175:diagnosis; D013923:Thromboembolism / Q000097:blood* / Q000175:diagnosis
chemical_list: D002135:Calcium-Binding Proteins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human