title: Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells.
abstract: Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H2O2-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage.
authors.affiliation: Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul, South Korea. dsmin@cmc.cuk.ac.kr
authors.forename: D S
authors.initials: DS
authors.lastname: Min
authors.forename: K S
authors.initials: KS
authors.lastname: Shin
authors.forename: E G
authors.initials: EG
authors.lastname: Kim
authors.forename: S R
authors.initials: SR
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Yoon
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.forename: Y H
authors.initials: YH
authors.lastname: Jo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D051379:Mice; D010739:Phospholipase D / Q000096:biosynthesis*; D018243:Teratocarcinoma / Q000201:enzymology* / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D014212:Tretinoin; D000242:Cyclic AMP; D010739:Phospholipase D