title: Colicin V can be produced by lactic acid bacteria.
abstract: Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which recognize a double-glycine-type leader peptide on the immature colicin V bacteriocin. Replacement of the colicin V leader peptide by a signal peptide from the signal sequence-dependent bacteriocin divergicin A allowed expression of colicin V in lactic acid bacteria. This system may serve as a model for the heterologous expression of other small bacteriocins active against Gram-negative bacteria and other antibacterial peptides from lactic acid bacteria.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Canada.
authors.forename: J K
authors.initials: JK
authors.lastname: McCormick
authors.forename: T R
authors.initials: TR
authors.lastname: Klaenhammer
authors.forename: M E
authors.initials: ME
authors.lastname: Stiles
mesh_terms: D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism; D003087:Colicins / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D007777:Lactobacillaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017045:Lactococcus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010957:Plasmids; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014169:Transformation, Bacterial
chemical_list: D001430:Bacteriocins; D003087:Colicins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C093812:dvnA protein, Carnobacterium divergens