title: Involvement of bradykinin B1 and B2 receptors in pulmonary leukocyte accumulation induced by Sephadex beads in guinea pigs.
abstract: The effects of selected bradykinin receptor antagonists on leukocyte infiltration into the lungs were studied in a model of guinea pig lung inflammation induced by the intravenous injection of Sephadex beads. The bradykinin B1 receptor antagonist, [Leu8]desArg9-BK (40 mg kg(-1) 24 h(-1)) and the bradykinin B2 receptor antagonist, DArg[Hyp3,Thi5,DTic7,Oic8]BK (code name HOE 140; 4 mg kg(-1) 24 h(-1)), administered intravenously by osmotic pumps, significantly reduced eosinophil counts by 33% and 42% in bronchoalveolar fluid, respectively. HOE 140 decreased neutrophil counts by 35%. LysLys[Hyp3,Igl5,D-Igl7,Oic8]desArg9BK+ ++ (code name B 9858), a newly described bradykinin B1 receptor antagonist, administered intraperitoneally (1 mg kg(-1)), decreased eosinophil and neutrophil counts by 45% in bronchoalveolar fluid. D-Arg[Hyp3,Igl5,D-Igl7,Oic8]BK (code name B 9430), a non-selective bradykinin B1/B2 receptor antagonist, also administered intraperitoneally (1 mg kg(-1)), decreased eosinophil and macrophage counts by 62% and 80% in bronchoalveolar fluid. These results suggest that bradykinin B1 and B2 receptors are involved in leukocyte recruitment in our model of lung inflammation.
authors.affiliation: Institut de Pharmacologie de Sherbrooke, Faculté de Médecine, Université de Sherbrooke, QC, Canada.
authors.forename: M S
authors.initials: MS
authors.lastname: Perron
authors.forename: F
authors.initials: F
authors.lastname: Gobeil
authors.forename: S
authors.initials: S
authors.lastname: Pelletier
authors.forename: D
authors.initials: D
authors.lastname: Regoli
authors.forename: P
authors.initials: P
authors.lastname: Sirois
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology; D065168:Bradykinin Receptor Antagonists; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D003911:Dextrans; D006168:Guinea Pigs; D007202:Indicators and Reagents; D007249:Inflammation / Q000209:etiology / Q000473:pathology*; D007609:Kallidin / Q000031:analogs & derivatives / Q000494:pharmacology; D007962:Leukocytes / Q000473:pathology*; D008171:Lung Diseases / Q000209:etiology / Q000473:pathology*; D008297:Male; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000502:physiology*
chemical_list: C121351:B 9430; C121350:B 9858; D065168:Bradykinin Receptor Antagonists; D003911:Dextrans; D007202:Indicators and Reagents; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D007609:Kallidin; C052787:kallidin, des-Arg(10)-; C065679:icatibant; C025614:sephadex; D001920:Bradykinin