title: The fusion protein of peste des petits ruminants virus is a hemolysin.
abstract: The fusion glycoprotein (F protein) of paramyxoviruses plays a vital role in virus-induced cytopathology. To explore the role of the F protein in peste des petits ruminants virus (PPRV)-induced cytopathology, the F protein of PPRV was purified by immunoaffinity chromatography. The purified F protein, when incubated with chicken erythrocytes, caused lysis suggesting that PPRV F protein is a hemolysin. Furthermore, the hemolysis can be inhibited by hyperimmune serum against F protein. The virus-induced cell fusion (syncytia) was also inhibited by the hyperimmune serum against the F protein. In summary, these results indicate that the purified PPRV F protein is biologically active and is involved in virus-induced hemolysis, cell-fusion and the initiation of infection.
authors.affiliation: Department of Veterinary Microbiology, Veterinary College, Bangalore, India.
authors.forename: L R
authors.initials: LR
authors.lastname: Devireddy
authors.forename: R
authors.initials: R
authors.lastname: Raghavan
authors.forename: S
authors.initials: S
authors.lastname: Ramachandran
authors.forename: M S
authors.initials: MS
authors.lastname: Shaila
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006461:Hemolysis; D018107:Peste-des-petits-ruminants virus / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D006460:Hemolysin Proteins; D014760:Viral Fusion Proteins