title: Strain-dependent induction of allergic rhinitis without adjuvant in mice.
abstract: BACKGROUND
  To date, no murine models have been reported to show the induction of both antigen-specific IgE and nasal eosinophilia, two of the major hallmarks of allergic rhinitis, after local sensitization in the absence of adjuvants, a phenomenon which reflects natural exposure. In this report, we attempted to establish a murine model representing an initiation of allergic rhinitis.
  
  
  METHODS
  BALB/c, CBA/J, and C57BL/6 mice were sensitized intranasally to Schistosoma mansoni egg antigen (SEA) solely. After repeated sensitization, serum Ab titers, nasal eosinophilia, and cytokine production by nasal lymphocytes were determined.
  
  
  RESULTS
  BALB/c mice produced SEA-specific IgE after repeated sensitization. High-dose sensitization to SEA induced IgE production in CBA/J mice, while C57BL/6 mice did not show the production throughout the period observed, suggesting that IgE production was regulated genetically. BALB/c mice also exhibited nasal eosinophilia after the nasal challenge. In addition, nasal lymphocytes sensitized with SEA intranasally produced significant amount of IL-5 in vitro.
  
  
  CONCLUSIONS
  These results suggest that intranasal sensitization with SEA in the absence of adjuvants induces a Th2 immune reaction, reflecting the hallmarks of the initiation of allergic rhinitis both in vivo and in vitro, which is genetically regulated.
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Okano
authors.forename: K
authors.initials: K
authors.lastname: Nishizaki
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: M M
authors.initials: MM
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: A R
authors.initials: AR
authors.lastname: Satoskar
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: D A
authors.initials: DA
authors.lastname: Harn
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004527:Egg Proteins / Q000276:immunology; D004802:Eosinophilia / Q000139:chemically induced / Q000276:immunology; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis; D015848:Interleukin-5 / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology; D013045:Species Specificity
chemical_list: D004527:Egg Proteins; D007074:Immunoglobulin G; D015848:Interleukin-5; D007073:Immunoglobulin E