Role of leukocyte influx in tissue prostaglandin H synthase-2 overexpression induced by phorbol ester and arachidonic acid in skin.
The accumulation of neutrophils and mononuclear cells is a characteristic feature of 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema. This cell influx was accompanied by the enhancement of eicosanoid tissue levels and prostaglandin H synthase-2 (PGHS-2) overexpression. Sialidase treatment, which affects the structure of selectins and inhibits leukocyte influx, significantly reduced eicosanoid and PGHS-2 levels and edema. In contrast, skin PGHS-2 overexpression induced by arachidonic acid (AA) application was not affected by sialidase treatment. These results suggest that PGHS-2 overexpression induced by TPA could be induced by AA and/or AA metabolite release by leukocyte infiltrated during the inflammatory process.
Sánchez
T
T
Department of Physiology, School of Pharmacy, Barcelona University, Spain.
Moreno
J J
JJ
D000208:Acute Disease; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001692:Biological Transport; D002273:Carcinogens / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D051546:Cyclooxygenase 2; D003872:Dermatitis / Q000201:enzymology / Q000276:immunology; D003879:Dermatologic Agents / Q000494:pharmacology; D004487:Edema / Q000201:enzymology / Q000209:etiology; D007527:Isoenzymes / Q000096:biosynthesis*; D007962:Leukocytes / Q000502:physiology*; D051379:Mice; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D003879:Dermatologic Agents; D007527:Isoenzymes; D016718:Arachidonic Acid; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D013755:Tetradecanoylphorbol Acetate