Quantification of the flavonoid-degrading bacterium Eubacterium ramulus in human fecal samples with a species-specific oligonucleotide hybridization probe.
To investigate the occurrence of the flavonoid-degrading bacterium Eubacterium ramulus in the human intestinal tract, an oligonucleotide probe designated S-S-E.ram-0997-a-A-18 was designed and validated, with over 90 bacterial strains representing the dominant described human fecal flora. Application of S-S-E. ram-0997-a-A-18 to fecal samples from 20 subjects indicated the presence of E. ramulus in each individual tested in numbers from 4.4 x 10(7) to 2.0 x 10(9) cells/g of fecal dry mass. Six fecal E. ramulus isolates were recognized by S-S-E.ram-0997-a-A-18 but exhibited different band patterns when analyzed by randomly amplified polymorphic DNA.
Simmering
R
R
Institut für Ernährungsforschung, Universität Potsdam, 14558 Bergholz-Rehbrücke, Germany.
Kleessen
B
B
Blaut
M
M
D001483:Base Sequence; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D005051:Eubacterium / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005419:Flavonoids / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015345:Oligonucleotide Probes / Q000235:genetics; D013045:Species Specificity
D005419:Flavonoids; D015345:Oligonucleotide Probes