title: Alkaline phosphatase activity in plasma of pregnant chagasic patients.
abstract: The kinetic properties of plasma placental alkaline phosphatase patients with Chagas' disease were studied. When Cl2Mg was used as activator the same increase of activity (17-20%) was found in the chagasic and non chagasic groups. The enzyme was not inhibited by F-ion in any of the groups. No significant differences were detected between the two groups (chagasic and non chagasic) when the enzyme was treated with inhibitors such as EDTA and L-phenylalanine. However, when the CN-ion was used, the enzyme of the normal pregnant women followed a Michaelian curve, whereas in the chagasic group a sigmoideal plot was observed. Thus, the Hill coefficient was 1.1 for the normal group and over 1.5 for the chagasic.
authors.affiliation: IIa. Cátedra de Histología, Embriología y Genética, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
authors.forename: M J
authors.initials: MJ
authors.lastname: Sartori
authors.forename: S
authors.initials: S
authors.lastname: Lin
authors.forename: R E
authors.initials: RE
authors.lastname: Fretes
authors.forename: L
authors.initials: L
authors.lastname: Ruiz Moreno
authors.forename: L
authors.initials: L
authors.lastname: Goldemberg
authors.forename: S P
authors.initials: SP
authors.lastname: de Fabro
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood* / Q000187:drug effects / Q000378:metabolism; D014355:Chagas Disease / Q000097:blood / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004793:Enzyme Reactivators / Q000494:pharmacology; D005260:Female; D006801:Humans; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D004793:Enzyme Reactivators; D000469:Alkaline Phosphatase