Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication.
Replication of adenovirus (Ad) DNA depends on interactions between three viral and three cellular proteins. Human transcription factors NFI and Oct-1 recruit the Ad DNA polymerase to the origin of DNA replication as a complex with the Ad protein primer pTP. High affinity and specificity DNA binding to recognition sites in this origin by the transcription factors stimulate and stabilize pre-initiation complex formation to compensate for the low binding specificity of the pTP/pol complex. In this review, we discuss the properties of NFI and Oct-1 and the mechanism by which they enhance initiation of DNA replication. We propose a model that describes the dynamics of initiation and elongation as well as the assembly and disassembly of the pre-initiation complex.
de Jong
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Laboratory for Physiological Chemistry and Centre for Biomedical Genetics, Utrecht University, Utrecht, The Netherlands.
van der Vliet
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D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology*; D001483:Base Sequence; D002478:Cells, Cultured / Q000821:virology; D017124:Conserved Sequence; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology; D051863:Host Cell Factor C1; D006801:Humans; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D050811:Octamer Transcription Factor-1; D014157:Transcription Factors / Q000502:physiology; D014779:Virus Replication / Q000502:physiology*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D051839:NFI Transcription Factors; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D014157:Transcription Factors; C080333:transcription factor nuclear factor 1