Interactions between peripherin and neurofilaments in cultured cells: disruption of peripherin assembly by the NF-M and NF-H subunits.
Neurofilaments are the principal intermediate filament type expressed by neurons. They are formed by the co-assembly of three subunits: NF-L, NF-M, and NF-H. Peripherin is another intermediate filament protein expressed mostly in neurons of the peripheral nervous system. In contrast to neurofilaments, peripherin can self-assemble to establish an intermediate filament network in cultured cells. The co-expression of neurofilaments and peripherin is found mainly during development and regeneration. We used SW13 cells devoid of endogenous cytoplasmic intermediate filaments to assess the exact assembly characteristics of peripherin with each neurofilament subunit. Our results demonstrate that peripherin can assemble with NF-L. In contrast, the co-expression of peripherin with the large neurofilament subunits interferes with peripherin assembly. These results confirm the existence of interactions between peripherin and neurofilaments in physiological conditions. Moreover, they suggest that perturbations in the stoichiometry of neurofilaments can have an impact on peripherin assembly in vivo.
Beaulieu
J M
JM
Centre for Research in Neurosciences, McGill University, The Montreal General Hospital Research Institute, QC, Canada.
Robertson
J
J
Julien
J P
JP
D000818:Animals; D002478:Cells, Cultured; D004247:DNA; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000378:metabolism; D064531:Peripherins; D014162:Transfection; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C578994:PRPH protein, human; D064531:Peripherins; D014746:Vimentin; C044882:neurofilament protein L; C051774:neurofilament protein H; C061195:neurofilament protein M; D004247:DNA