Isotope dilution high-performance liquid chromatography/tandem mass spectrometry method for quantifying urinary metabolites of atrazine, malathion, and 2,4-dichlorophenoxyacetic acid.
We have developed an isotope dilution high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for quantifying the urinary metabolites of the pesticides atrazine, malathion, and 2,4-dichlorophenoxyacetic acid (2,4-D). Urine samples are extracted with an organic solvent, and the organic fraction is concentrated. The concentrate is then analyzed using HPLC/MS/MS. The limits of detection for the metabolites are less than 0.5 microgram/L (parts per billion) in 10 mL of urine, with a high degree of accuracy and precision.
Beeson
M D
MD
National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30341, USA. mwb9@cdc.gov
Driskell
W J
WJ
Barr
D B
DB
D015084:2,4-Dichlorophenoxyacetic Acid / Q000652:urine*; D001280:Atrazine / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006540:Herbicides / Q000652:urine*; D006801:Humans; D007306:Insecticides / Q000652:urine*; D008294:Malathion / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical
D006540:Herbicides; D007306:Insecticides; D015084:2,4-Dichlorophenoxyacetic Acid; D001280:Atrazine; D008294:Malathion