Inhibition of HIV-1 integration by mono- & bi-functionalized triple helix forming oligonucleotides.
HIV-1 DNA integration is carried out by integrase, a viral protein which binds to specific sequences located on both extremities of the HIV-1 DNA LTR. Inhibition of integration was observed with submicromolar concentrations of mono- or bifunctionalized 11-mer oligonucleotide-intercalators, which were designed to form an alternate strand triple helix with the U5 LTR end containing two adjacent purine tracts on opposite strands 5'-GGAAAATCTCT-3'/3'-CCTTTTAGAGA-5'.
Brodin
P
P
CNRS-UMR 1772, Institut Gustave Roussy, Villejuif, France.
Gottikh
M
M
Auclair
C
C
Mouscadet
J F
JF
D001483:Base Sequence; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000502:physiology*; D009841:Oligonucleotides / Q000494:pharmacology*; D016662:Virus Integration / Q000187:drug effects*
D009841:Oligonucleotides