title: Granulocyte colony-stimulating factor and drugs elevating extracellular adenosine act additively to enhance the hemopoietic spleen colony formation in irradiated mice.
abstract: The effects of combined administration of two drugs elevating extracellular adenosine, namely dipyridamole (DP) and adenosine monophosphate (AMP), and granulocyte colony-stimulating factor (G-CSF) on hemopoietic stem cells in vivo were investigated. The experiments were performed on mice using the endogenous spleen colony formation in gamma-irradiated animals as an endpoint. The results have shown that DP and AMP act additively with G-CSF to enhance spleen colony formation and thus the erythroid repopulation of the spleen. These findings indicate that the signaling pathways of G-CSF and drugs elevating extracellular adenosine can interact at the level of primitive hemopoietic stem cells. The enhancement of hemopoiesis-stimulating effects of G-CSF by DP and AMP, which are low-priced and clinically available drugs, could improve the cost-effectiveness of the therapy with G-CSF.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.
authors.forename: M
authors.initials: M
authors.lastname: Hofer
authors.forename: M
authors.initials: M
authors.lastname: Pospísil
authors.forename: J
authors.initials: J
authors.lastname: Netíková
authors.forename: V
authors.initials: V
authors.lastname: Znojil
authors.forename: J
authors.initials: J
authors.lastname: Vácha
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002452:Cell Count; D003037:Cobalt Radioisotopes; D003114:Colony-Forming Units Assay; D004176:Dipyridamole / Q000494:pharmacology; D015672:Erythroid Precursor Cells / Q000166:cytology; D005110:Extracellular Space / Q000378:metabolism*; D005720:Gamma Rays; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology*; D014916:Whole-Body Irradiation
chemical_list: D003037:Cobalt Radioisotopes; D016179:Granulocyte Colony-Stimulating Factor; D000249:Adenosine Monophosphate; D004176:Dipyridamole; D000241:Adenosine