An unusual pattern of invertase activity development in the thermophilic fungus Thermomyces lanuginosus.
In the thermophilic fungus Thermomyces lanuginosus, invertase displays an unusual pattern of development: the induced activity begins to diminish even before any substantial quantity of sucrose has been utilized or an appreciable amount of biomass has been produced. Despite this pattern of invertase activity, neither the growth rate nor the final mycelial yield is affected adversely. T. lanuginosus invertase is a thiol protein and the enzyme is active when specific sulfhydryl group(s) is in the reduced state. Measurements of reduced coenzyme and glutathione pools in sucrose-growth mycelia excluded oxidative stress as the primary reason for the observed decline in invertase activity. Rather, this unusual pattern of invertase is considered to be due to its localization in the hyphal tips. At the early stage of growth, the number of hyphal tips per unit mass of mycelium is maximum, whereas at later times their numbers do not increase in proportion to the biomass. As a result invertase activity shows an apparent inverse relationship with biomass. The enzyme activity disappears when the inducing carbon source is consumed and growth is completed.
Chaudhuri
A
A
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
Bharadwaj
G
G
Maheshwari
R
R
D000818:Animals; D018533:Biomass; D005243:Feces / Q000382:microbiology; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006736:Horses; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification; D009249:NADP / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014198:Trehalase / Q000378:metabolism; D043324:beta-Fructofuranosidase
D013438:Sulfhydryl Compounds; D009249:NADP; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D014198:Trehalase; D005978:Glutathione; D019803:Glutathione Disulfide