A new HPLC technique for the separation of methocarbamol enantiomers.
We have developed a stereoselective high-performance liquid chromatography technique for analytical separation of methocarbamol enantiomers. Precolumn derivatization was performed at room temperature using (-)-menthylchloroformate as a chiral reagent in the presence of pyridine as catalyst. The resulting diastereomers were separated on two Resolve C18 columns connected in series. The mobile phase was phosphate buffer (pH 7.5)-acetonitrile (50: 50, v/v) at a flow rate of 1 mL min(-1). UV detection was set at 274 nm. The optimum amount of reagent and the maximum peak intensity of the diastereomers were determined. The resolution of the diastereomers was satisfactory (alpha = 1.04) under the conditions used.
Farsam
H
H
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Iran. farsam@nrcgeb.ac.ir
Souri
E
E
Gharavi
N
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005561:Formates; D008721:Methocarbamol / Q000032:analysis* / Q000737:chemistry; D012997:Solvents; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors; D014050:Toluene
D005561:Formates; D012997:Solvents; D008721:Methocarbamol; C059663:menthyl chloroformate; D014050:Toluene