title: Combined genetic and physical map of the complex genome of Agrobacterium tumefaciens.
abstract: A combined genetic and physical map of the Agrobacterium tumefaciens A348 (derivative of C58) genome was constructed to address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two nonhomologous chromosomes. The combined map confirms the two-chromosome genomic structure and the correspondence of the initial genetic maps to the circular chromosome. The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was missed by genetic mapping studies.
authors.affiliation: Department of Biology, University of Richmond, Richmond, Virginia 23173, USA. bgoodner@richmond.edu
authors.forename: B W
authors.initials: BW
authors.lastname: Goodner
authors.forename: B P
authors.initials: BP
authors.lastname: Markelz
authors.forename: M C
authors.initials: MC
authors.lastname: Flanagan
authors.forename: C B
authors.initials: CB
authors.lastname: Crowell
authors.forename: J L
authors.initials: JL
authors.lastname: Racette
authors.forename: B A
authors.initials: BA
authors.lastname: Schilling
authors.forename: L M
authors.initials: LM
authors.lastname: Halfon
authors.forename: J S
authors.initials: JS
authors.lastname: Mellors
authors.forename: G
authors.initials: G
authors.lastname: Grabowski
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D016680:Genome, Bacterial; D020161:Physical Chromosome Mapping