[Effects of PGMS on the functions of human platelets and endothelial cells].
OBJECTIVE
To study the effects of Propylene glycol mannte sulfate (PGMS), a heparin-like substance on the functions of platelets and endothelial cells.


METHODS
Using 125I labeled monoclonal antibody to measure the expression of GMP-140 on the surface of human platelets; and using naphthol blue black stain method to study the growth of human umbilical vein endothelial cells (HUVEC) and human umbilical vein smooth muscle cells (HUVSMG).


RESULTS
PGMS significantly inhibited thrombin stimulated expression of GMP-140 on the surface of human platelets. IC50 was 127 micrograms/ml. Indomethacin also inhibited GMP-140 expression, but its effect was weaker than that of PGMS with the IC50 of 502 micrograms/ml. PGMS did not affect the growth of HUVSMC. On the contrary, it significantly promoted the growth of HUVEC at very low concentration (1.6 micrograms/ml).


CONCLUSIONS
PGMS showes anticoagulative effects on both platelets and endothelial cells and it might well be developed as a new antithrombotic and anticoagulateve agent.
Weng
J
J
Institute of Basic Medical Sciences, CAMS, Beijing.
Lin
Y
Y
Zhu
X
X
Wang
Z
Z
D000925:Anticoagulants / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D019007:P-Selectin / Q000096:biosynthesis*; D011409:Propylene Glycols / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors; D014471:Umbilical Veins / Q000166:cytology
D000925:Anticoagulants; D019007:P-Selectin; D011409:Propylene Glycols; D013917:Thrombin; D007213:Indomethacin