title: Catabolism of the methyl derivatives of adenosine and cytidine in staphylococci.
abstract: Products of 1-methyladenosine, 2'-O-methyladenosine, 2'-O-methylcytidine, and 5-methylcytidine catabolism by resting cells of Staphylococcus aureus and Staphylococcus intermedius were chromatographically separated. The methyl group in 1-methyladenosine protected the adenosine derivative from deamination by S. intermedius but it did not protect N-glycosidic bond from cleavage by S. intermedius and S. aureus. The methyl group in 2'-O-methyladenosine and 2'-O-methylcytidine protected the N-glycosidic bond from cleavage by S. aureus and S. intermedius but it did not protect the adenosine and cytidine derivatives from deamination by S. intermedius. 5-Methylcytidine was converted by the common route in which 5-methylcytidine was first deaminated to ribothymidine which was cleaved to yield thymine. S. intermedius deaminated the purine and pyrimidine ribonucleosides adenosine, 2'-O-methyladenosine, cytidine, and 5-methylcytidine. Pyrimidine ribonucleosides (cytidine, 5-methyl-cytidine) were deaminated only slowly and purine ribonucleosides (adenosine, 2'-O-methyladenosine) not at all by S. aureus.
authors.affiliation: Department of Medical Microbiology, Medical University of Gdańsk, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Krasuski
authors.forename: M
authors.initials: M
authors.lastname: Marlewski
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D003562:Cytidine / Q000031:analogs & derivatives* / Q000378:metabolism; D007288:Inosine / Q000031:analogs & derivatives / Q000378:metabolism; D013210:Staphylococcus / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
chemical_list: C024341:2'-O-methyladenosine; C002230:1-methyladenosine; C012433:1-methylinosine; C052203:2'-O-methylcytidine; D007288:Inosine; D003562:Cytidine; D000241:Adenosine; C016568:5-methylcytidine; D014529:Uridine