HSP70 expression in granulocytes and lymphocytes of patients with polytrauma: comparison with plasma glutamine.
Heat shock proteins (HSP's) are a set of conserved proteins which confer tolerance to stress. These proteins play a major role in the pathophysiology of infection and inflammation. Induction of HSP's before onset of sepsis is able to reduce or prevent organ damage and death. GLN is known to influence the expression of HSP70 in different cell types. In this work we tried to find out if there is an association between plasma GLN levels and HSP70 expression in immune cells. We investigated six polytraumatized patients and a control group of six healthy donors. HSP70 expression was investigated by western blot analysis and immune-histochemistry. We demonstrated that granulocytes and lymphocytes behave differently in the expression of HSP70 in polytraumatized patients. In healthy donors both lymphocytes and granulocytes showed a pronounced expression of HSP70. In contrast, most of the polytraumatized patients showed no HSP70 expression in granulocytes. In lymphocytes of these patients, however, a pronounced expression similar to that of healthy volunteers was observed. Plasma glutamine levels were reduced in all patients and at normal range in healthy donors. These results suggest that lymphocytes and granulocytes behave different when confronted with a reduction of plasma GLN levels.
Weingartmann
G
G
Surgical Research Laboratories, AKH 8G9-05, Waehringer Guertel 18-20, Vienna, A-1090, Austria.
Oehler
R
R
Derkits
S
S
Oismüller
C
C
Függer
R
R
Roth
E
E
D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D005973:Glutamine / Q000097:blood*; D006098:Granulocytes / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009104:Multiple Trauma / Q000276:immunology*
D018840:HSP70 Heat-Shock Proteins; D005973:Glutamine