Nuclear translocation of the p65 subunit of NF-kappaB in L5178Y sublines differing in antioxidant defense.
We examined the induction of nuclear translocation of the p65 subunit of NF-kappaB in L5178Y (LY) cells. We used two LY sublines which are inversely cross-sensitive to hydrogen peroxide and x-rays: LY-R cells are radioresistant and oxidant-sensitive, whereas LY-S cells are radiosensitive and oxidant-resistant. Hydrogen peroxide, phorbol ester and x-rays caused a marked translocation of p65-NF-kappaB in LY-R cells and a weak translocation in LY-S cells. By manipulating the antioxidant defense status, we obtained an alteration in the p65-NF-kappaB translocation induction in LY-R cells. A similar effect was achieved with lovastatin pretreatment (25 microM, 24 h, 37 degrees C). The response of LY-S cells under all these conditions was considerably weaker. We conclude that differential nuclear translocation of p65-NF-kappaB in LY sublines is not related to the lethal effect of the activating, damaging agent; rather it is due to the more efficient antioxidant defense in LY-S than in LY-R cells.
Sochanowicz
B
B
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland.
Szumiel
I
I
Gradzka
I
I
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007940:Leukemia L5178 / Q000188:drug therapy / Q000235:genetics* / Q000532:radiotherapy; D008148:Lovastatin / Q000494:pharmacology; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014178:Translocation, Genetic / Q000187:drug effects* / Q000528:radiation effects*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D000970:Antineoplastic Agents; D000975:Antioxidants; D016328:NF-kappa B; D004247:DNA; D008148:Lovastatin; D006861:Hydrogen Peroxide