Natural synthesis of a DNA-binding protein from the C-terminal domain of DNA gyrase A in Borrelia burgdorferi.
We have identified a 34 kDa DNA-binding protein with an HU-like activity in the Lyme disease spirochete Borrelia burgdorferi. The 34 kDa protein is translated from an abundant transcript initiated within the gene encoding the A subunit of DNA gyrase. Translation of the 34 kDa protein starts at residue 499 of GyrA and proceeds in the same reading frame as full-length GyrA, resulting in an N-terminal-truncated protein. The 34 kDa GyrA C-terminal domain, although not homologous, substitutes for HU in the formation of the Type 1 complex in Mu transposition, and complements an HU-deficient strain of Escherichia coli. This is the first example of constitutive expression of two gene products in the same open reading frame from a single gene in a prokaryotic cellular system.
Knight
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SW
Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA.
Samuels
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DS
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D010583:Bacteriophage mu / Q000378:metabolism; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D034982:Integration Host Factors; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C099188:HupA protein, bacteria; D034982:Integration Host Factors; D009698:Nucleoproteins; D012333:RNA, Messenger; C490937:hupA protein, E coli; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II