Balloon angioplasty induces heat shock protein 70 in human blood vessels.
Balloon angioplasty produces a mechanically induced injury to the blood vessel wall. Heat shock protein 70 (HSP70) is a molecular chaperone whose expression can be induced by chemical or thermal stress. Thus, we hypothesized that the mechanical injury associated with balloon angioplasty would lead to increases in the expression of HSP70 in vascular smooth muscle. Segments of popliteal and trifurcation vessels from above-the-knee amputations were subject to transluminal balloon angioplasty, excised, and placed in organ cultures. Neighboring vessel that was not subjected to balloon angioplasty served as controls. Some vessels were treated with sodium arsenite (positive control, known to induce HSP70 expression). The vessels were homogenized and the proteins were separated by gel electrophoresis and transferred to Immobilon. Western blots with an antibody specific for the inducible form of HSP70 were analyzed by densitometry. Our results showed that HSP70 expression can be induced by the mechanical injury associated with balloon angioplasty in human atherosclerotic vessels.
Kirby
L B
LB
Department of Surgery, Medicine (Institute for Molecular Medicine and Genetics), Medical College of Georgia, Augusta, GA 30912-4002, USA.
Mondy
J S
JS
Brophy
C M
CM
D000704:Analysis of Variance; D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000906:Antibodies; D018053:Arsenites / Q000494:pharmacology; D001161:Arteriosclerosis / Q000378:metabolism / Q000628:therapy; D015153:Blotting, Western; D046508:Culture Techniques; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005263:Femoral Artery / Q000293:injuries / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D009131:Muscle, Smooth, Vascular / Q000293:injuries / Q000378:metabolism; D011150:Popliteal Artery / Q000293:injuries / Q000378:metabolism*; D019275:Radiopharmaceuticals; D017670:Sodium Compounds / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D000906:Antibodies; D018053:Arsenites; D018840:HSP70 Heat-Shock Proteins; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D017670:Sodium Compounds; D013439:Sulfhydryl Reagents; C017947:sodium arsenite