title: Effects of cold acclimation on the activity levels of creatine kinase, lactate dehydrogenase and lactate dehydrogenase isoenzymes in various tissues of the rat.
abstract: The effects of cold acclimation on the activity levels of creatine kinase, lactate dehydrogenase and lactate dehydrogenase isoenzymes in various tissues/ organs of the rat (Rattus norvegicus) were investigated. Male Sprague-Dawley rats were divided into two groups. One group was housed at 4+/-1 degrees C (experimental group) and the other at 24+/-1 degrees C (control group) for six months. The rats were housed in single cages and had access to food and water ad libitum. The tissues/organs investigated were heart, liver, lung, kidney, gastrocnemius muscle and interscapular brown adipose tissue as well as serum. With the exception of lung, (which showed a decrease of 24%) total creatine kinase activity levels were significantly increased (P< 0.05) in all the tissues/organs investigated (17-51%) as well as serum (34%), in cold acclimated animals. Cold acclimation also resulted in significantly increased (P< 0.05) activity levels of lactate dehydrogenase in all the tissues/organs investigated (14-24%) as well as serum (35%). Cold exposure resulted in an increase of the activity levels of all the detectable isoenzymes of lactate dehydrogenase, although not always significant, in all the tissues/organs investigated as well as serum. The M(4)tetramer of lactate dehydrogenase was the only detectable isoenzyme in serum.
authors.affiliation: Department of Biochemistry and Microbiology, University of Zululand, Kwa Dlangezwa, 3886, South Africa.
authors.forename: S E
authors.initials: SE
authors.lastname: Terblanche
authors.forename: T C
authors.initials: TC
authors.lastname: Masondo
authors.forename: W
authors.initials: W
authors.lastname: Nel
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000201:enzymology; D000818:Animals; D003081:Cold Climate / Q000009:adverse effects*; D003402:Creatine Kinase / Q000378:metabolism*; D007527:Isoenzymes; D007668:Kidney / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase