[Standardization and quality control in the evaluation of proliferation parameters in T1T2, N0N1, M0 breast cancer: multicentric retrospective study II. DNA-ploidy and S-phase fraction].
As part of a clinical research project, proliferative parameters were studied in primary breast cancer: standardization and technical validation of thymidine kinase (TK), thymidylate synthase (TS) and protein tyrosine kinase (PTK) are described. A total of 633 frozen tumor specimens, available in four institutions, was analyzed in three flow cytometry laboratories for DNA content and percentage of S-phase cells (%S) measurement. 1) The standardization step consisted in developing a common protocol for sample preparation; then, common cell suspensions were analyzed in order to perform an inter-laboratory control. Objective guidelines were elaborated to interpret DNA histograms in breast carcinoma. 2) DNA-aneuploidy was observed in 61% of cases of the retrospective series. Compared with DNA-aneuploid tumors, mean %S was significantly lower in case of DNA-diploidy (respectively: 6.4% and 2.2%, p < 0.001). When compared between the four institutions, %S distributions did not differ significantly. 3) %S is strongly correlated with TK, TS and PTK and high percentages were also observed in high grade tumors or tumor without hormone receptors. These results show that a standardization in using flow cytometers and DNA software allows multicenter studies.
Chassevent
A
A
Centre Paul-Papin, 2, rue Moll, 49036 Angers Cedex.
Jourdan
M L
ML
Ferrero-Poüs
M
M
Colonna
M
M
Romain
S
S
Spyratos
F
F
Roth
H
H
Bolla
M
M
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D002455:Cell Division / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009367:Neoplasm Staging; D011003:Ploidies; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D011786:Quality Control; D012189:Retrospective Studies; D016196:S Phase / Q000235:genetics*; D013937:Thymidine Kinase / Q000032:analysis; D013940:Thymidylate Synthase / Q000032:analysis
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D011505:Protein-Tyrosine Kinases