A novel motif mediates the targeting of the Arabidopsis COP1 protein to subnuclear foci.
The constitutive photomorphogenesis 1 (COP1) protein of Arabidopsis thaliana accumulates in discrete subnuclear foci. To better understand the role of subnuclear architecture in COP1-mediated gene expression, we investigated the structural motifs of COP1 that mediate its localization to subnuclear foci using mutational analysis with green fluorescent protein as a reporter. In a transient expression assay, a subnuclear localization signal consisting of 58 residues between amino acids 120 and 177 of COP1 was able to confer speckled localization onto the heterologous nuclear NIa protein from tobacco etch virus. The subnuclear localization signal overlaps two previously characterized motifs, a cytoplasmic localization signal and a putative alpha-helical coiled-coil domain that has been implicated in COP1 dimerization. Moreover, phenotypically lethal mutations in the carboxyl-terminal WD-40 repeats inhibited localization to subnuclear foci, consistent with a functional role for the accumulation of COP1 at subnuclear sites.
Stacey
M G
MG
Department of Botany, The University of Tennessee, Knoxville, Tennessee 37996-1100, USA.
von Arnim
A G
AG
D000595:Amino Acid Sequence; D017360:Arabidopsis; D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D013347:Subcellular Fractions / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D008164:Luminescent Proteins; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases