title: Quantitative determination of indapamide in pharmaceuticals and urine by high-performance liquid chromatography with amperometric detection.
abstract: A high-performance liquid chromatographic method with amperometric detection for the determination of the diuretic indapamide using a muBondapak C18 column is developed. The mobile phase consists of an acetonitrile-water mixture (45:55, 5 mM) in KH2PO4-K2HPO4 (pH 4.0). The compound is monitored at +1200 mV with an amperometric detector equipped with a glassy carbon working electrode. A liquid-liquid or solid-liquid extraction is performed prior to chromatographic analysis to avoid the interferences found in urine matrix. Percentages of recovery are 88.3 +/- 5.6 and 82.9 +/- 7.8 for liquid-liquid and solid-liquid extraction, respectively. The developed method has a linear concentration range from 25 to 315 ng/mL with a reproducibility in terms of relative standard deviation of 4% for a concentration level of 0.5 microgram/mL and a quantitation limit of 1 ng/mL. The method is applied to the determination of indapamide in tablets and urine obtained from hypertensive patients after the ingestion of Tertensif (indapamide 2.5 mg).
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias, Universidad del País Vasco/EHU, Bilbao, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Legorburu
authors.forename: R M
authors.initials: RM
authors.lastname: Alonso
authors.forename: R M
authors.initials: RM
authors.lastname: Jiménez
authors.forename: E
authors.initials: E
authors.lastname: Ortiz
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics / Q000032:analysis* / Q000652:urine; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000652:urine; D007190:Indapamide / Q000032:analysis* / Q000652:urine*; D008297:Male; D008875:Middle Aged; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D004232:Diuretics; D004364:Pharmaceutical Preparations; D007190:Indapamide