Detection of glycopeptide-resistant enterococci in routine diagnostic faeces specimens.
Faeces received in a diagnostic laboratory were screened for glycopeptide-resistant enterococci (GRE) on modified Lewisham medium, with and without enrichment in Enterococcosel broth. Colonization by GRE was detected in 102/838 patients (12.2%). In 74 (73%) of colonized patients GRE were detected by both methods and in 28 (27%) they were detected only after enrichment. The carriage rate in hospitalized patients was 32% (93/289) compared with 2.3% (11/425) in the community (GP patients and food-handlers). Carriage of GRE increased with age. Clostridium difficile isolation was associated with GRE colonization, odds ratio 6.76 (P<0.001). Fifty-nine percent (60/102) of the GRE had the VanA phenotype and 41% (42/102) had the VanB phenotype. In the community VanA predominated (91%), whereas 64% (57/89) of the isolates from hospitalised patients were of the VanB phenotype.
Taylor
M E
ME
Manchester PHL, Withington Hospital, West Didsbury, Manchester, M20 2LR.
Oppenheim
B A
BA
Chadwick
P R
PR
Weston
D
D
Palepou
M F
MF
Woodford
N
N
Bellis
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002353:Carrier State / Q000453:epidemiology*; D002648:Child; D002675:Child, Preschool; D016360:Clostridioides difficile / Q000302:isolation & purification; D003955:Diagnostic Tests, Routine; D016983:Enterococcus / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006020:Glycopeptides; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology*; D006801:Humans; D007223:Infant; D008131:London / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010641:Phenotype
D000900:Anti-Bacterial Agents; D006020:Glycopeptides