title: Evaluation of a new anti-HCV-assay kit for anti-HCV screening in early childhood.
abstract: Sera of 20 children falsely identified as positive for hepatitis C virus antibody (Anti-HCV) by a second generation anti-HCV-assay kit (Imucheck-HCV Ab "Kokusai") were re-tested using a new third generation anti-HCV-assay kit (Imucheck x F-HCV C50 Ab "Kokusai"). Seventeen of the samples were reclassified as negative and only three remained positive. Changing well solids in the anti-HCV-assay kit from casein to bovine serum albumin appears to have improved the false-positive rate, most likely as a result of decreased non-specific adsorption of casein antibodies.
authors.affiliation: Department of Pediatrics, Nagoya City University Medical School, Nagoya, Japan. sugiyama@med.nagoya-cu.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: F
authors.initials: F
authors.lastname: Mizutani
authors.forename: K
authors.initials: K
authors.lastname: Terabe
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Maniwa
authors.forename: Y
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
mesh_terms: D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D008403:Mass Screening; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic