Effects of hepatocyte growth factor (HGF) and activin A on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture.
To study the mechanisms of morphogenesis in salivary gland regeneration, we have established the RSMG-1 cell line derived from submandibular gland (SMG) of 10-wk-old Wistar female rats in serum-free culture. Our finding that RSMG-1 cells originated from duct cells was based on morphology and immunohistochemical results. In three-dimensional serum-free collagen gel culture, HGF induced branching morphogenesis of RSMG-1 cells. Histological examination revealed that HGF-induced branching structure exhibited well-formed lumina. This morphology closely resembles that found in vivo. The cells also expressed activin A. Exogenously added activin A at a high concentration reduced HGF-induced branching morphogenesis. These findings suggest that the morphogenesis of the salivary gland is modulated by HGF and activin A. Our results show that the RSMG-1 cell line may be useful in studies of salivary gland regeneration.
Furue
M
M
Department of Oral Biochemistry, Kanagawa Dental College, Yokosuka City, Japan.
Okamoto
T
T
Hayashi
H
H
Sato
J D
JD
Asashima
M
M
Saito
S
S
D028341:Activins; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D005782:Gels; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology*; D007265:Inhibins / Q000494:pharmacology / Q000502:physiology*; D009024:Morphogenesis; D009218:Myosins / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000096:biosynthesis; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
D016895:Culture Media, Serum-Free; D005782:Gels; D009418:S100 Proteins; D028341:Activins; D007265:Inhibins; D017228:Hepatocyte Growth Factor; D003094:Collagen; D009218:Myosins