title: Soluble interleukin-2R alpha test kits measure different epitopes: sIL-2R alpha test kits comparison with a newly developed inhouse sIL-2R alpha sandwich ELISA.
abstract: Cytokines are a group of low molecular weight glycoproteins important in cellular signaling for various responses such as activation and proliferation. They separate the cellular responses into Th1 and Th2 pathways, where each pathway releases its own cytokine group. Interleukins are an important section of the cytokine group, and consist of a number of members with their respective receptors such as interleukin-2 and interleukin-2 receptor (IL-2 and IL-2R, respectively). In this study, a soluble interleukin-2 receptor (sIL-2R alpha) sandwich ELISA was developed and tested with different samples. This method was compared with commercial test kits that measure sIL-2R alpha molecule. The results showed that different kits measure different epitopes on this molecule.
authors.affiliation: Department of Immunology, University of Sheffield, Northern General Hospital, UK.
authors.forename: K
authors.initials: K
authors.lastname: Kazim
authors.forename: G
authors.initials: G
authors.lastname: Wild
authors.forename: A
authors.initials: A
authors.lastname: Ward
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018984:Epitopes, T-Lymphocyte / Q000097:blood*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008958:Models, Molecular; D011933:Reagent Kits, Diagnostic; D015375:Receptors, Interleukin-2 / Q000097:blood*; D012995:Solubility
chemical_list: D018984:Epitopes, T-Lymphocyte; D011933:Reagent Kits, Diagnostic; D015375:Receptors, Interleukin-2