Pattern formation in the absence of cell proliferation: tissue-specific regulation of cell cycle progression by string (stg) during Drosophila eye development.
During Drosophila eye development, the posterior-to-anterior movement of the morphogenetic furrow coordinates cell cycle progression with the early events of pattern formation. The cdc25 phosphatase string (stg) has been proposed to contribute to the synchronization of retinal precursors anterior to the furrow by driving cells in G(2) through mitosis and into a subsequent G(1). Genetic and molecular analysis of Drop (Dr) mutations suggests that they represent novel cis-regulatory alleles of stg that inactivate expression in eye. Retinal precursors anterior to the furrow lacking stg arrest in G(2) and fail to enter mitosis, while cells within the furrow accumulate high levels of cyclins A and B. Although G(2)-arrested cells initiate normal pattern formation, the absence of stg results in retinal patterning defects due to the recruitment of extra photoreceptor cells. These results demonstrate a requirement for stg in cell cycle regulation and cell fate determination during eye development.
Mozer
B A
BA
Laboratory of Molecular Biology, National Institutes of Neurological Diseases and Stroke, National Institutes of Health, Building 36, Room 3D02, Bethesda, Maryland 20892, USA. BMozer@codon.nih.gov
Easwarachandran
K
K
D000483:Alleles; D000818:Animals; D019521:Body Patterning; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002455:Cell Division; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins; D005123:Eye / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D017344:Genes, Insect; D005816:Genetic Complementation Test; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000502:physiology*; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D017027:Protein Tyrosine Phosphatases; D012313:RNA / Q000235:genetics / Q000378:metabolism
D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D012313:RNA; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; C059217:stg protein, Drosophila