title: Rapid preparation of denatured double-stranded DNA templates for sequencing.
abstract: This article proposes protocol for rapid preparation (ds) DNA templates for sequencing based on double-stranded DNA denaturation and its recovery by extraction with Wizard DNA purification resin (Promega). This method is an alternative to commonly used procedure employing denatured-DNA recovery by ethanol precipitation.
authors.affiliation: Department of Microbiology, University of Gdansk, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Kaczorowski
authors.forename: M
authors.initials: M
authors.lastname: Sektas
authors.forename: W
authors.initials: W
authors.lastname: Szybalski
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D015525:Fatty Acids, Omega-3 / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D015525:Fatty Acids, Omega-3; C060580:Promega; D004247:DNA