title: High concentrations of sodium chloride facilitate the use of immobilized chymotrypsin for separating virgin forms of specific trypsin inhibitors.
abstract: It has been shown that specific trypsin inhibitors exhibit also antichymotrypsin activity in the presence of high NaCl concentrations. Taking advantage of this phenomenon a simple procedure of separation of the virgin forms of trypsin inhibitors from squash seeds and porcine pancreas (Kazal) was elaborated. In a typical experiment the inhibitor sample was loaded onto immobilized chymotrypsin equilibrated with 5 M NaCl at pH 8. After washing out unadsorbed material the virgin forms of inhibitors could be eluted either with water, buffer pH 8.0 or 0.02 M citrate buffer pH 2.6 containing no NaCl.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Wilimowska-Pelc
authors.forename: Z
authors.initials: Z
authors.lastname: Olichwier
authors.forename: J
authors.initials: J
authors.lastname: Kowalska
authors.forename: A
authors.initials: A
authors.lastname: Gałuszka
authors.forename: W
authors.initials: W
authors.lastname: Szuszkiewicz
authors.forename: A
authors.initials: A
authors.lastname: Polanowski
authors.forename: T
authors.initials: T
authors.lastname: Wilusz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000737:chemistry*; D013552:Swine; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004800:Enzymes, Immobilized; D014361:Trypsin Inhibitors; D012965:Sodium Chloride; D002918:Chymotrypsin