Ploidy analysis and S-phase fraction determination by flow cytometry in anembryonic pregnancy and spontaneous abortions.
The nuclear DNA content of 20 anembryonic pregnancies was studied by flow cytometry from paraffin embedded tissue blocks. An abnormal amount of DNA content was found in 8 of the cases. This was a significantly higher percentage than encountered in spontaneous abortions studied by the same population (40 and 9%, respectively, p < 0.05). The S-phase fraction in anembryonic pregnancies was lower than in spontaneous abortions (22.4 +/- 12.7 and 35.4 +/- 6.8, respectively, p < 0.01). The results indicated that abnormal embryogenesis with grave chromosomal aberrations may play a major role in the etiology of anembryonic pregnancy.
Ozeren
M
M
Department of Obstetrics and Gynecology, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
Aydemir
V
V
Tekelioglu
Y
Y
Topçuoglu
K
K
Bozkaya
H
H
D000022:Abortion, Spontaneous / Q000235:genetics* / Q000473:pathology; D000328:Adult; D002869:Chromosome Aberrations / Q000235:genetics* / Q000473:pathology; D025063:Chromosome Disorders; D004247:DNA / Q000032:analysis / Q000235:genetics; D004622:Embryo, Mammalian; D005260:Female; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D011003:Ploidies; D011247:Pregnancy / Q000235:genetics*; D016196:S Phase / Q000502:physiology*
D004247:DNA