Improved soft-agar colony assay in a fluid processing apparatus.
The standard method for quantitating bone marrow precursor cells has been to count the number of colony-forming units that form in semisolid (0.3%) agar. Recently we adapted this assay for use in hardware, the Fluid Processing Apparatus, that is flown in standard payload lockers of the space shuttle. When mouse or rat macrophage colony-forming units were measured with this hardware in ground-based assays, we found significantly more colony growth than that seen in standard plate assays. The improved growth correlates with increased agar thickness but also appears to be due to properties inherent to the Fluid Processing Apparatus. This paper describes an improved method for determining bone marrow macrophage precursor numbers in semisolid agar.
Forsman
A D
AD
Division of Biology, Kansas State University, Manhattan 66506-4901, USA.
Herpich
A R
AR
Chapes
S K
SK
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division; D003114:Colony-Forming Units Assay / Q000295:instrumentation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008809:Mice, Inbred C3H; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight