Deficient antibody-dependent cellular cytotoxicity against human immunodeficiency virus (HIV)-expressing target cells in perinatal HIV infection.
Peripheral blood mononuclear cells (PBMC) of human immunodeficiency virus (HIV)-infected children, age-matched HIV-seronegative controls, and HIV-infected asymptomatic and symptomatic adults were compared for their ability to mediate antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) cell-mediated cytotoxicity against target cells expressing HIV or herpes simplex virus (HSV) antigens. Target cells consisted of CD4 lymphocytes purified from PBMC of HIV-seronegative adults and incubated with the IIIB strain of HIV, HUT78 cells chronically infected with IIIB, and HSV-infected human fibroblasts. PBMC of asymptomatic HIV-infected adults were generally able to lyse CD4 cells expressing HIV antigens. Direct correlation was found between the magnitude of lysis and absolute CD4 cell counts in these individuals. In contrast to these results, PBMC from HIV-infected children were generally unable to lyse IIIB-expressing CD4 cells, regardless of the children's clinical status, age, or absolute CD4 cell counts. Cells from HIV-seronegative adults and children did not directly lyse these target cells either but, in contrast to cells of HIV-seropositive children, were able to mediate cell lysis when serum from an HIV-seropositive adult was added. However, effector cells from these HIV-infected children were able to mediate both ADCC against HSV-infected fibroblasts and NK cell-mediated cytotoxicity against IIIB-infected HUT78 cells. Reduced ability of PBMC from vertically HIV-infected children to mediate ADCC against HIV antigen-expressing CD4 cells may contribute to rapid progression to AIDS.
Ziegner
U
U
Division of Allergy, Immunology, and Infectious Diseases, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA. uziegner@mednet.ucla.edu
Campbell
D
D
Weinhold
K
K
Frank
I
I
Rutstein
R
R
Starr
S E
SE
D000328:Adult; D000367:Age Factors; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D015483:HIV Antibodies / Q000032:analysis / Q000097:blood; D015658:HIV Infections / Q000276:immunology* / Q000635:transmission; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D015497:HIV-1; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000821:virology
D000939:Epitopes; D015483:HIV Antibodies