title: Calcium channel subtypes and exocytosis in chromaffin cells: a different view from the intact rat adrenal.
abstract: In the intact rat adrenal gland perfused with an oxygenated Krebs-bicarbonate solution at 37 degrees C, the electrical field stimulation of splanchnic nerves (100 V, 0.5 ms duration, 10 Hz during 10 s) produced transient catecholamine release peaks that were reproduced in subsequent stimuli, applied at 8-min intervals. Omega-Conotoxin GVIA (0.3 microM) caused only a modest inhibition of the secretory response, suggesting that the N-subtype of voltage-dependent Ca2+ channels are scarcely involved in such a response. Both omega-conotoxin MVIIC (1 microM) and furnidipine (1 microM) halved the secretion, suggesting that the L- and P/Q-types of Ca2+ channels were involved. N-type Ca2+ channels appear to be involved in the maintenance of secretion in response to sustained stimulus since omega-conotoxin GVIA (0.3 microM) reduced the catecholamine output to 28%. When secretion was elicited by acetylcholine (10 microM), furnidipine reduced the catecholamine release by 50% and omega-conotoxin MVIIC by 40%, whereas omega-conotoxin GVIA did not modify the response. The K+-induced secretory responses (23.6 mM K+, 15 s) were reduced 75% by furnidipine and 45% by omega-conotoxin MVIIC, indicating that this type of stimulation preferentially recruited L-type channels. These data show that electrical stimulation recruits Ca2+ channel subtypes different from those recruited by direct depolarization of chromaffin cells.
authors.affiliation: Unidad de Farmacología, Facultad de Medicina, Universidad de la Laguna, Tenerife, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Santana
authors.forename: P
authors.initials: P
authors.lastname: Michelena
authors.forename: R
authors.initials: R
authors.lastname: Jaén
authors.forename: A G
authors.initials: AG
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: Borges
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D020746:Calcium Channels, L-Type; D020864:Calcium Channels, N-Type; D002395:Catecholamines / Q000378:metabolism; D019439:Chromaffin Cells / Q000378:metabolism / Q000502:physiology*; D004558:Electric Stimulation; D005089:Exocytosis; D005260:Female; D066298:In Vitro Techniques; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000109:Acetylcholine; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D020864:Calcium Channels, N-Type; D002395:Catecholamines; D011188:Potassium