title: Photonuclease activity of Taylor's blue.
abstract: Taylor's blue (1,9-dimethylmethylene blue, DMMB+) associates with DNA, at least in part, through intercalation as is evidenced from the red shift in the absorption maximum, diminution of the fluorescence, and induced circular dichroism in the presence of nucleic acid. Irradiation of DMMB+/covalently closed circular supercoiled phiX174 phage DNA complex at lambda > 520 nm leads to DNA nicking in a dose-dependent manner.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393, USA.
authors.forename: T
authors.initials: T
authors.lastname: Mohammad
authors.forename: H
authors.initials: H
authors.lastname: Morrison
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D007364:Intercalating Agents / Q000378:metabolism / Q000494:pharmacology*; D008751:Methylene Blue / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000378:metabolism / Q000494:pharmacology; D013057:Spectrum Analysis
chemical_list: D007364:Intercalating Agents; D017319:Photosensitizing Agents; D004247:DNA; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue