title: Mechanism and specificity of RNA cleavage by chemical ribonucleases.
abstract: Cleaving of model RNA substrates by chemical ribonucleases constructed by conjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine was investigated. Similarly to RNase A, the chemical RNases produce fragments with 5' hydroxy-group and 3'-cyclophosphate. The cleavage occurs as the catalytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved by one molecule of RNase mimic.
authors.affiliation: Institute of Bioorganic Chemistry, Novosibirsk, Russia.
authors.forename: N
authors.initials: N
authors.lastname: Beloglazova
authors.forename: A
authors.initials: A
authors.lastname: Vlassov
authors.forename: D
authors.initials: D
authors.lastname: Konevetc
authors.forename: V
authors.initials: V
authors.lastname: Sil'nikov
authors.forename: M
authors.initials: M
authors.lastname: Zenkova
authors.forename: R
authors.initials: R
authors.lastname: Giege
authors.forename: V
authors.initials: V
authors.lastname: Vlassov
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D006868:Hydrolysis; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000235:genetics
chemical_list: C031540:M-protein, influenza virus; D014763:Viral Matrix Proteins; D012313:RNA; D012260:Ribonucleases