title: NMR diffusion and relaxation study of drug-protein interaction.
abstract: In this work, NMR diffusion and relaxation measurements are applied to the study of the interaction between the anti-inflammatory drug salicylate and the human serum albumin (HSA) in solutions. The self-diffusion coefficients and the spin-lattice relaxation rates of salicylate are measured as a function of the concentration. The dissociation constant, Kd, for drug/HSA complexes and the number of binding sites, n, are evaluated.
authors.forename: R S
authors.initials: RS
authors.lastname: Luo
authors.forename: M L
authors.initials: ML
authors.lastname: Liu
authors.forename: X A
authors.initials: XA
authors.lastname: Mao
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D004058:Diffusion; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D012980:Sodium Salicylate / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004364:Pharmaceutical Preparations; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D012980:Sodium Salicylate