Intranasal administration of synthetic recombinant peptide-based vaccine protects mice from infection by Schistosoma mansoni.
Schistosomiasis is the cause of a chronic debilitating disease which accounts for significant mortality and morbidity every year, especially in tropical and subtropical areas. An epitope derived from the protective surface protein 9B-Ag of Schistosoma mansoni, designated 9B peptide-1, was previously showed to be protective in mice when conjugated to bovine serum albumin and administered subcutaneously in complete Freund's adjuvant. In this work, this protective peptide was expressed in the flagellin of a Salmonella vaccine strain, and the isolated recombinant flagella were used for immunization of mice. Since during the invasion of the parasite into the host the schistosomula migrate first to the lungs, the intranasal route of administration was employed in order to halt the parasite at an early stage of the infection. Such intranasal immunization with this peptide expressed in flagellin, without the addition of adjuvants, resulted in a significant humoral response and also led to protection against challenge infection, manifested as a reduction of the worm burden by an average of 42%.
Ben-Yedidia
T
T
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Tarrab-Hazdai
R
R
Schechtman
D
D
Arnon
R
R
D000281:Administration, Intranasal; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001700:Biomphalaria; D004195:Disease Models, Animal; D005408:Flagellin / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000469:parasitology / Q000517:prevention & control*; D014018:Tissue Distribution; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D010455:Peptides; D011993:Recombinant Fusion Proteins; D018074:Vaccines, Conjugate; D014614:Vaccines, Synthetic; D005408:Flagellin