title: Kinetics of CheY phosphorylation by small molecule phosphodonors.
abstract: The chemotaxis response regulator CheY can acquire phosphoryl groups either from its associated autophosphorylating protein kinase, CheA, or from small phosphodonor molecules such as acetyl phosphate. We report a stopped-flow kinetic analysis of CheY phosphorylation by acetyl phosphate. The results show that CheY has a very low affinity for this phosphodonor (K(s)&z.Gt;0.1 M), consistent with the conclusion that, whereas CheY provides catalytic functions for the phosphotransfer reaction, the CheA kinase may act simply to increase the effective phosphodonor concentration at the CheY active site.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Da Re
authors.forename: D
authors.initials: D
authors.lastname: Deville-Bonne
authors.forename: T
authors.initials: T
authors.lastname: Tolstykh
authors.forename: M
authors.initials: M
authors.lastname: V ron
authors.forename: J B
authors.initials: JB
authors.lastname: Stock
mesh_terms: D000577:Amides / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods; D005453:Fluorescence; D006639:Histidine / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D000072236:Methyl-Accepting Chemotaxis Proteins; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism*; D010756:Phosphoric Acids / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D000577:Amides; D001426:Bacterial Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D010755:Organophosphates; D010756:Phosphoric Acids; D006639:Histidine; C011632:acetyl phosphate; C011067:phosphoramidic acid; C005436:phosphohistidine