title: Migration of mesonephric cells into the mammalian gonad depends on Sry.
abstract: In mammals, the primary step in male sex determination is the initiation of testis development which depends on the expression of the Y-linked testis determining gene, Sry. The mechanisms by which Sry controls this process are unknown. Studies showed that cell migration from the adjacent mesonephros only occurs into XY gonads; however, it was not known whether this effect depended on Sry, another Y-linked gene, or the presence of one versus two X chromosomes. Here we provide genetic proof that Sry is the only Y-linked gene necessary for cell migration into the gonad. Cell migration from the mesonephros into the differentiating gonad is consistently associated with Sty's presence and with testis cord formation, suggesting that cell migration plays a critical role in the initiation of testis cord development. The induction of cell migration represents the earliest signaling pathway yet assigned to Sry.
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA. b.capel@cellbio.duke.edu
authors.forename: B
authors.initials: B
authors.lastname: Capel
authors.forename: K H
authors.initials: KH
authors.lastname: Albrecht
authors.forename: L L
authors.initials: LL
authors.lastname: Washburn
authors.forename: E M
authors.initials: EM
authors.lastname: Eicher
mesh_terms: D000818:Animals; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004627:Embryonic Induction / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008650:Mesonephros / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009687:Nuclear Proteins; D009924:Organ Culture Techniques; D051876:Sex-Determining Region Y Protein; D013737:Testis / Q000196:embryology; D014157:Transcription Factors; D014998:Y Chromosome
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors