Alternative exon 3 splicing of the human major protein zero gene in white blood cells and peripheral nerve tissue.
The major protein zero (MPZ) is involved in peripheral myelin folding. Using nested reverse transcription-PCR, we amplified several fragments of MPZ mRNAs in white blood cells and in peripheral nerve tissue. Cloning of PCR products revealed the existence of three alternative splicing patterns: one resulted in the complete loss of exon 3 and two others induced partial skipping of the exon 3 sequence. All three alternative splicing mechanisms produced a frame-shift and created an identical premature stop codon in exon 4. We conclude that the existence of these MPZ RNA transcript variants may be the result of deliberate splicing decisions and may have functional implications in the cell.
Besançon
R
R
Laboratoire de Génétique Moléculaire Humaine, Faculté de Pharmacie, 8 avenue Rockefeller, F-69008, Lyon, France. besancon@rockefeller.univ-lyon1.fr
Prost
A L
AL
Konecny
L
L
Latour
P
P
Petiot
P
P
Boutrand
L
L
Kopp
N
N
Mularoni
A
A
Chamba
G
G
Vandenberghe
A
A
D000199:Actins / Q000235:genetics; D000328:Adult; D017398:Alternative Splicing; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics; D018388:Codon, Terminator; D017931:DNA Primers; D005091:Exons / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D018993:Myelin P0 Protein / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D017933:Peripheral Nervous System / Q000737:chemistry* / Q000166:cytology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D000199:Actins; D018388:Codon, Terminator; D017931:DNA Primers; D018993:Myelin P0 Protein; D012313:RNA