Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 2. Protein adsorption and platelet adhesion.
Protein adsorption and platelet adhesion from human plasma on polysulfone (PSf) membranes modified with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer were studied. The modification was carried out by blending of the MPC polymer in the PSf. The amount of protein adsorbed on the PSf/MPC polymer blend membrane was significantly decreased with an increase in the composition of the blended MPC polymer. The distribution of the specific proteins adsorbed on the membrane surface was also determined by a gold-colloid immunoassay. Albumin, gamma-globulin and fibrinogen were observed on every membrane surface after contact with plasma. However, in the case of the blended membrane, the density of the adsorbed proteins decreased compared with that of original PSf membrane. That is, the MPC polymer blended in the membrane could function as a protein-adsorption-resistant additive. The number of platelets adhered on the PSf membrane was reduced, and change in the morphology of adherent platelets was also suppressed by the modification with the MPC polymer. Therefore, the PSf/MPC polymer blend membrane had improved blood compatibility compared with the PSf membrane.
Ishihara
K
K
Department of Materials Science, Graduate School of Engineering, The University of Tokyo, Japan.
Fukumoto
K
K
Iwasaki
Y
Y
Nakabayashi
N
N
D000327:Adsorption; D001798:Blood Proteins / Q000493:pharmacokinetics*; D006801:Humans; D008567:Membranes, Artificial; D010743:Phospholipids / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011109:Polymethacrylic Acids; D013450:Sulfones / Q000494:pharmacology*; D013499:Surface Properties
D001798:Blood Proteins; D008567:Membranes, Artificial; D010743:Phospholipids; D011109:Polymethacrylic Acids; D013450:Sulfones