Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen.
Schistosoma circulating antigens were used for the detection of active infection. Anti-S. mansoni IgG2a monoclonal antibody (MAb) designated C5C4 was generated. The target epitope of this MAb was detected in adult worms, eggs, and cercariae antigenic extracts of S. mansoni and S. haematobium, had a molecular size of 63 kD, and was not detected in Fasciola hepatica and Ascaris. In addition, a 50-kD degradation product was identified only in the urine of infected individuals. Analysis by high-performance liquid chromatography of the purified antigen demonstrated only one peak. The 63-kD antigen was characterized as a protein containing 40.4% hydrophobic, 7.5% acidic, and 8.8% basic amino acids. The C5C4 MAb was used in a Fast Dot-ELISA for rapid and simple diagnosis of human schistosomiasis. The 63-kD circulating antigen was detected in 92% of urine samples from 330 S. mansoni-infected individuals, with 16% false-positive results among 130 noninfected individuals.
Attallah
A M
AM
Biotechnology Research Center, New Damietta City, Egypt.
Yones
E
E
Ismail
H
H
El Masry
S A
SA
Tabll
A
A
Elenein
A A
AA
El Ghawalby
N A
NA
D000293:Adolescent; D000328:Adult; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D017164:Ascaris lumbricoides / Q000276:immunology; D015153:Blotting, Western; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005210:Fasciola hepatica / Q000276:immunology; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D051379:Mice; D008875:Middle Aged; D008970:Molecular Weight; D010270:Parasite Egg Count; D012007:Rectum / Q000469:parasitology; D012548:Schistosoma haematobium / Q000276:immunology / Q000302:isolation & purification*; D012550:Schistosoma mansoni / Q000276:immunology / Q000302:isolation & purification*; D012553:Schistosomiasis haematobia / Q000175:diagnosis* / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000276:immunology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth