title: Structural determination of a 5-acetamido-3,5,7, 9-tetradeoxy-7-(3-hydroxybutyramido)-L-glycero-L-manno-nonulos onic acid-containing homopolysaccharide isolated from Sinorhizobium fredii HH103.
abstract: The structure of a polysaccharide from Sinorhizobium fredii HH103 has been determined. This polysaccharide was isolated by following the protocol for lipopolysaccharide extraction. On the basis of monosaccharide analysis, methylation analysis, fast atom bombardment MS, matrix-assisted laser desorption ionization MS, electron-impact high-resolution MS, one-dimensional (1)H-NMR and (13)C-NMR and two-dimensional NMR experiments, the structure was shown to consist of a homopolymer of a 3:1 mixture of 5-acetamido-3,5,7, 9-tetradeoxy-7-[(R)- and (S)-3-hydroxybutyramido]-l-glycero-l-manno-nonulosonic acid. The sugar residues are attached via a glycosidic linkage to the OH group of the 3-hydroxybutyramido substituent and thus the monomers are linked via both glycosidic and amidic linkages. In contrast with the Sinorhizobium K-antigens previously reported, which are composed of a disaccharide repeating unit, the K-antigen polysacharide of S. fredii HH103 is a homopolysaccharide.
authors.affiliation: Departamento de Qu approximately ímica Orgánica, Facultad de Qu approximately ímica, Universidad de Sevilla, 41071 Sevilla, Spain. agil@cica.es
authors.forename: A M
authors.initials: AM
authors.lastname: Gil-Serrano
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez-Carvajal
authors.forename: P
authors.initials: P
authors.lastname: Tejero-Mateo
authors.forename: J L
authors.initials: JL
authors.lastname: Espartero
authors.forename: M
authors.initials: M
authors.lastname: Menendez
authors.forename: J
authors.initials: J
authors.lastname: Corzo
authors.forename: J E
authors.initials: JE
authors.lastname: Ruiz-Sainz
authors.forename: A M
authors.initials: AM
authors.lastname: BuendíA-Clavería
mesh_terms: D002236:Carbohydrate Conformation; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry*; D020579:Sinorhizobium / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011135:Polysaccharides, Bacterial; D012794:Sialic Acids; C047276:5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)nonulosonic acid