The synergistic action of ethanol and nerve growth factor in the induction of neuronal nitric oxide synthase.
Ethanol alone had no effect on neuronal nitric oxide synthase (nNOS) expression in PC12 cells. However, in the presence of nerve growth factor (NGF), nNOS expression was amplified (threefold, P < 0.05), compared to NGF alone. This increase was eliminated with pretreatment of PC12 cells with staurosporine, suggesting that the effects of ethanol on nNOS expression are mediated by a protein kinase C-dependent pathway.
Phung
Y T
YT
Department of Pediatrics, University of California at San Francisco, 94143-0106, USA.
Black
S M
SM
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D003720:Densitometry / Q000379:methods; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D016716:PC12 Cells / Q000187:drug effects; D011493:Protein Kinase C / Q000187:drug effects; D051381:Rats
D000431:Ethanol; D020932:Nerve Growth Factor; D019001:Nitric Oxide Synthase; D011493:Protein Kinase C