In vitro comparative evaluation under static conditions of the hemocompatibility of four types of tubing for cardiopulmonary bypass.
A comparative in vitro assessment of 4 types of tubing representative of the materials currently used in cardiopulmonary bypass (CPB) procedures was conducted under static conditions using liquid extracts of the materials or direct contact with fresh human blood or serum. The parameters monitored were biomarkers of coagulation and fibrinolytic cascades, the complement system and cell activation. Silicone and PVC tubing were shown to be non-cytotoxic and non-hemolytic. Heparin-coated PVC tubing did present a certain degree of cytotoxicity especially when in direct contact. Thrombosis was found to be significantly lower with the same heparin-coated material. To a lesser extent, platinum-cured silicone also showed a reduced thrombotic tendency. None of the materials activated platelets or the complement system. With platinum-cured silicone tubing, constant and lower leukocyte adhesion was evidenced at the different experimental time points. This could reflect reduced cell activation.
Harmand
M F
MF
INSERM U443, Université Bordeaux II, France.
Briquet
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F
D001672:Biocompatible Materials / Q000592:standards*; D001769:Blood / Q000187:drug effects*; D001777:Blood Coagulation / Q000187:drug effects; D002315:Cardiopulmonary Bypass / Q000295:instrumentation*; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003167:Complement Activation / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006461:Hemolysis; D006493:Heparin / Q000009:adverse effects; D006801:Humans; D010984:Platinum / Q000494:pharmacology; D011143:Polyvinyl Chloride / Q000009:adverse effects; D012826:Silicone Elastomers / Q000009:adverse effects; D013927:Thrombosis / Q000139:chemically induced / Q000517:prevention & control
D001672:Biocompatible Materials; D012826:Silicone Elastomers; D010984:Platinum; D011143:Polyvinyl Chloride; D006493:Heparin