[The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis].
The specific to 3 types of Cry genes primers containing inosine were constructed to find crystal insecticidal protein Bacillus thuringiensis genes using PCR. A number of new B. thuringiensis strains isolated in Ukraine were investigated using these PCR primers. As a results, Cry genes were found, some of them were identified and demonstrated high homology to Cry1Ba2 and Cry1Bc genes.
Rymar'
S E
SE
Isakova
I A
IA
Kirilenko
S D
SD
Kordium
V A
VA
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D001517:Coleoptera; D017931:DNA Primers / Q000138:chemical synthesis*; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006460:Hemolysin Proteins; D007288:Inosine; D007306:Insecticides; D007915:Lepidoptera; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D014455:Ukraine
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D007288:Inosine