Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50.
Polyadenylation of messenger RNA precursors requires a complex protein machinery that is closely integrated with the even more complex transcriptional apparatus. Here a polyadenylation factor, CstF-50 (cleavage stimulation factor), is shown to interact in vitro and in intact cells with a nuclear protein of previously unknown function, BRCA1-associated RING domain protein (BARD1). The BARD1-CstF-50 interaction inhibits polyadenylation in vitro. BARD1, like CstF-50, also interacts with RNA polymerase II. These results indicate that BARD1-mediated inhibition of polyadenylation may prevent inappropriate RNA processing during transcription, perhaps at sites of DNA repair, and they reveal an unanticipated integration of diverse nuclear events.
Kleiman
F E
FE
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Manley
J L
JL
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011061:Poly A / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051135:Rad51 Recombinase; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers; D039221:mRNA Cleavage and Polyadenylation Factors
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D012322:RNA Precursors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D011061:Poly A; C102449:BARD1 protein, human; D044767:Ubiquitin-Protein Ligases; C495153:RAD51 protein, human; D012319:RNA Polymerase II; D051135:Rad51 Recombinase