title: Applications for the new electrochemiluminescent (ECL) and biosensor technologies.
abstract: Biosensor and electrochemiluminescent (ECL) assays are replacing enzyme-linked immunosorbent assays (ELISAs) at Schering-Plough as immunoassays of choice to monitor cytokine levels and detect anti-cytokine antibody responses during cytokine therapy. These new assays provide increased sensitivity and a better correlation with biological assays. Biosensor assays using the BIACORE 2000 (BIACORE, Uppsala, Sweden) are being adopted to support preclinical and clinical trials for the detection of antibodies capable of binding to IL-10 and IL-4. Significant advantages when using a biosensor assay are that real-time and label-free detection permit increased throughput and direct detection of binding interactions which enables detection of low affinity antibodies that are not detected by ELISA. The ECL assays using the ORIGEN Analyser (IGEN, Gaithersburg, MD) that we have implemented to replace existing ELISAs for quantification of serum IL-10 and serum interferon alfa levels are more sensitive and less subject to matrix effects. Data obtained during the validation of these assays are described.
authors.affiliation: Schering-Plough Research Institute, Kenilworth, NJ 07033, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Swanson
authors.forename: S J
authors.initials: SJ
authors.lastname: Jacobs
authors.forename: D
authors.initials: D
authors.lastname: Mytych
authors.forename: C
authors.initials: C
authors.lastname: Shah
authors.forename: S R
authors.initials: SR
authors.lastname: Indelicato
authors.forename: R W
authors.initials: RW
authors.lastname: Bordens
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D016207:Cytokines / Q000032:analysis* / Q000276:immunology / Q000627:therapeutic use; D004563:Electrochemistry / Q000379:methods* / Q000706:statistics & numerical data; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D007370:Interferon Type I / Q000097:blood; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D008163:Luminescent Measurements; D011994:Recombinant Proteins / Q000097:blood; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D016207:Cytokines; D007370:Interferon Type I; D011994:Recombinant Proteins; D016753:Interleukin-10