Effects of veratrine and paeoniflorin on the isolated rat aorta.
The interactions and mechanisms between veratrine and paeoniflorin on the isolated rat aorta were studied. Veratrine (1x10(-6) to 1x10(-4) g/ml) could induce contraction on the isolated rat aorta in a concentration-related manner. Paeoniflorin had no effect on the isolated rat aorta. Pretreatment with prazosin (1x10(-6) M) and nifedipine (1x10(-6) M) but not yohimbine (1x10(-5) M) could decrease the tension of contraction induced by veratrine (1x10(-4) g/ml). Sodium nitroprusside (1x10(-4) M) could inhibit the contraction induced by veratrine (1x10(-4) g/ml) with or without endothelium, whereas methylene blue (5x10(-5) M) could increase the contraction induced by veratrine (1x10(-4) g/ml). Treatment with veratrine (1x10(-4) g/ml) could decrease the tension of contraction induced by norepinephrine (1x10(-6) M) or phenylephrine (1x10(-4) M). The inhibition of veratrine on norepinephrine-induced contraction was potentiated by L-arginine (1x10(-4) M) and reversed by L-NAME (1x10(-5) M). Paeoniflorin (1x10(-4) M) could decrease the tension of contraction induced by veratrine (1x10(-4) g/ml) and methylene blue (5x10(-5) M). The inhibition of paeoniflorin on veratrine was more potent on rat isolated aorta with endothelium than without endothelium. Ryanodine (1x10(-5) M) and Ca2+ -free medium could inhibit methylene blue-induced contraction. From the above results, the relaxation of veratrine on the norepinephrine-induced contraction might be related to the increase of NO and cGMP. The contraction of veratrine on the isolated rat aorta was via the increase of intracellular calcium which was inhibited by paeoniflorin.
Tsai
H Y
HY
Department of Pharmacology and Institute of Chinese Pharmaceutical Sciences, China Medical College, Taichung, Taiwan, ROC.
Lin
Y T
YT
Chen
C F
CF
Tsai
C H
CH
Chen
Y F
YF
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000187:drug effects*; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002118:Calcium / Q000378:metabolism*; D004347:Drug Interactions; D004727:Endothelium / Q000187:drug effects / Q000502:physiology; D005960:Glucosides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D039821:Monoterpenes; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014702:Veratrine / Q000494:pharmacology*; D006027:Glycosides
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002118:Calcium; D005960:Glucosides; D039821:Monoterpenes; D009638:Norepinephrine; D014662:Vasoconstrictor Agents; D014702:Veratrine; C015423:peoniflorin; D006027:Glycosides