Ras-mediated signaling pathway regulates the expression of a low-molecular-weight heat-shock protein in fission yeast.
In fission yeast, Schizosaccharomyces pombe, deficiency of ras1 gene causes an abnormal cell shape and abolishes mating ability. However, target genes of this signaling pathway are largely unknown because of the lack of an appropriate analysis system. To overcome this problem, we have started a novel project to categorize entire genes based on their expression levels under different growth conditions. Using this strategy, we screened genes whose expression levels were affected in the presence or absence of the ras1 gene product. For this purpose, we utilized high-density arrays of clones covering the entire genome of the fission yeast, and probed with labelled cDNA derived from various strains and growth conditions. Here, we demonstrate the detection of a low-molecular-weight heat-shock protein gene, hsp16, whose expression is very likely to be regulated by a ras-mediated signaling pathway, but not by the heat-shock response.
Danjoh
I
I
National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.
Fujiyama
A
A
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002478:Cells, Cultured; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D017343:Genes, Plant; D015698:Genomic Library; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013696:Temperature; D018631:ras Proteins / Q000378:metabolism*
D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; C491888:hsp16 protein, S pombe; D016203:CDC2 Protein Kinase; C492657:Ras1 protein, S pombe; D018631:ras Proteins