title: Empirical relationship between chiral selectivity and mobile phase modifier properties.
abstract: An empirical relationship was derived which relates properties of the mobile phase modifier to the chiral selectivity factor for a given analyte/chiral selector combination. Using carbon dioxide and heptane-based mobile phases, the effect of various mobile phase modifiers on Pirkle-type stationary phases may be accurately modeled using a two-parameter equation. Similar results are obtained using cellulosic stationary phases with carbon dioxide-based mobile phases. Modeling separations performed using heptane-based mobile phases with cellulosic stationary phases were not successful. The predictive ability of this modeling approach was demonstrated using novel modifiers and chiral analytes.
authors.affiliation: Dupont Pharmaceuticals Company, Chemical Process Research and Development, Chambers Works, Deepwater, NJ 08023, USA. john.a.blackwell@dupontpharma.com
authors.forename: J A
authors.initials: JA
authors.lastname: Blackwell
authors.forename: R W
authors.initials: RW
authors.lastname: Stringham
authors.forename: D
authors.initials: D
authors.lastname: Xiang
authors.forename: R E
authors.initials: RE
authors.lastname: Waltermire
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D012997:Solvents