Differentiation of convalescent animals from those vaccinated against foot-and-mouth disease by a peptide ELISA.
We have identified continuous antigenic determinants within the amino acid sequences of the conserved nonstructural region containing proteins 2C and 3ABC of foot-and-mouth disease virus which can distinguish between the sera from vaccinated and infected animals. An ELISA based on a 3B peptide gave a positive reaction with sera from cattle, pigs, sheep and guinea pigs infected with all seven serotypes of the virus, but not with sera from vaccinated animals. In experiments with cattle and pigs to determine the duration of the antibody response, positive reactions were obtained as late as one year after infection. The advantages of using peptides from the nonstructural viral proteins instead of recombinant proteins for differentiating vaccinees from infected animals include their exquisite specificity, nonreactivity with antibodies against host cell-derived proteins (e.g. E. coli and insect cell proteins), and their ease of preparation.
Shen
F
F
United Biomedical, Inc, Hauppauge, NY 11788, USA.
Chen
P D
PD
Walfield
A M
AM
Ye
J
J
House
J
J
Brown
F
F
Wang
C Y
CY
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle; D003289:Convalescence; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018604:Epitope Mapping; D005536:Foot-and-Mouth Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D006168:Guinea Pigs; D016056:Immunodominant Epitopes / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary; D012756:Sheep; D013552:Swine; D014611:Vaccination / Q000662:veterinary; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
C109754:3ABC protein, virus; D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines