Thermodynamic contribution of nucleoside modifications to yeast tRNA(Phe) anticodon stem loop analogs.
The determination of the structural and functional contributions of natural modified nucleosides to tRNA has been limited by lack of an approach that can systematically incorporate the modified units. We have produced a number of oligonucleotide analogs, of the anticodon of yeast tRNA(Phe) by, combining standard automated synthesis for the major nucleosides with specialty chemistries for the modified nucleosides. In this study, both naturally occurring and unnatural modified nucleotides were placed in native contexts. Each oligonucleotide was purified and the nucleoside composition determined to validate the chemistry. The RNAs were denatured and analyzed to determine the van't Hoff thermodynamic parameters. Here, we report the individual thermodynamic contributions for Cm, Gm, m1G, m5C, psi. In addition m5m6U, m1psi, and m3psi, were introduced to gain additional understanding of the physicochemical contribution of psi and m5C at an atomic level. These oligonucleotides demonstrate that modifications have measurable thermodynamic contributions and that loop modifications have global contributions.
Agris
P F
PF
Department of Biochemistry, North Carolina State University, Raleigh 27695-7622, USA. agris@bchserver.bch.ncsu.edu
Guenther
R
R
Sochacka
E
E
Newman
W
W
Czerwińska
G
G
Liu
G
G
Ye
W
W
Malkiewicz
A
A
D000926:Anticodon / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D012360:RNA, Transfer, Phe / Q000737:chemistry* / Q000302:isolation & purification; D013816:Thermodynamics
D000926:Anticodon; D012360:RNA, Transfer, Phe