Lymphocyte subsets in conjunctival mucosa-associated-lymphoid-tissue after exposure to retinal-S-antigen.
OBJECTIVE
To evaluate the immune cell subsets in conjunctival mucosa-associated-lymphoid-tissue (C-MALT) following challenge with antigen.


METHODS
Ten adult female Lewis rats were studied. Five rats received one drop (5 microL) of retinal S-antigen (500 microg/mL in phosphate buffered saline, PBS) instilled into the lower fornix twice daily for 10 consecutive days. Five rats received PBS only and served as controls for the experiment. Two days after the last instillation the animals were sacrificed and the orbital contents prepared for immunohistological staining. A panel of monoclonal antibodies was used: CD5, CD4, CD8, CD25, and CD45RA. The number of positive cells were counted in sections of epibulbar, forniceal, and tarsal conjunctiva.


RESULTS
There was a significant increase in the number of CD8+ T lymphocytes in the conjunctiva of animals receiving retinal S-antigen when compared to control animals.


CONCLUSIONS
Conjunctival instillation of retinal S-antigen causes an immune response in the C-MALT with a significant increase in the CD8+ T lymphocyte subset in this tissue. This response may be involved in the induction of tolerance to the encountered antigen.
Gormley
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PD
Larry A. Donoso Laboratory for Eye Research, Queen's Medical Centre, University Hospital, Nottingham, UK.
Powell-Richards
A O
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Azuara-Blanco
A
A
Donoso
L A
LA
Dua
H S
HS
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D019393:Arrestin; D016516:CD4-CD8 Ratio; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003230:Conjunctival Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D005260:Female; D016131:Lymphocyte Subsets / Q000276:immunology*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D019393:Arrestin