Detection of small round structured viruses in artificially contaminated water using filter adsorption and reverse transcription polymerase chain reaction.
The small round structured viruses (SRSV) are common causes of gastroenteritis worldwide. Fecally contaminated water is an important vehicle for transmission, but detection of SRSV in environmental samples has been hampered by the lack of sensitive detection methods. The present work describes the detection of SRSV in artificially contaminated deionized water and raw drinking water. SRSV-containing fecal extracts were added to water and virus was recovered by filter adsorption-elution, followed by flocculation. RNA was extracted and SRSV were detected by the use of reverse transcription polymerase chain reaction. The sensitivity of the method corresponded to a positive SRSV detection in 500 ml deionized water with an estimated concentration of 0.5-5 virus particles per ml.
Myrmel
M
M
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, Oslo. Mette.Myrmel@veths.no
Rimstad
E
E
Wasteson
Y
Y
Skjerve
E
E
D000327:Adsorption; D005243:Feces / Q000821:virology; D005374:Filtration; D005759:Gastroenteritis / Q000821:virology; D006801:Humans; D009663:Norwalk virus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014871:Water Microbiology