[The uptake of fluoroquinolone agent drugs by cultured human conjunctival epithelial cells and cultured SIRC rabbit corneal cells].
OBJECTIVE
To investigate the uptake of drugs by cultured human conjunctival epithelial cells (HCEC) and cultured rabbit corneal cell lines (SIRC).


METHODS
The drugs examined were ofloxacin (OFLX), lomefloxacin (LFLX), and norfloxacin (NFLX). The amount of drug uptake in the cultured cells that were exposed to the drugs was measured by high-performance liquid chromatography (HPLC).


RESULTS
The amount of LFLX, OFLX, and NFLX uptake in HCEC was 1.38 +/- 0.21 nmol/10(6) (mean +/- standard deviation) cells (5 min), 0.79 +/- 0.05 nmol/10(6) cells (5 min), and 0.56 +/- 0.03 nmol/10(6) cells (5 min), respectively. The amount of LFLX, OFLX, and NFLX uptake in SIRC was 1.08 +/- 0.18 nmol/10(6) cells (5 min), 0.58 +/- 0.17 nmol/10(6) cells (5 min), and 0.41 +/- 0.35 nmol/10(6) cells (5 min), respectively. The uptake levels of LFLX by both cultured cells were higher than those of OFLX and NFLX.


CONCLUSIONS
This method may be useful as a screening method for intraocular drug dynamics studies.
Fukuda
M
M
Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan.
Yoshitake
Y
Y
Sasaki
K
K
D000818:Animals; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D002478:Cells, Cultured; D003228:Conjunctiva / Q000378:metabolism*; D003315:Cornea / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D024841:Fluoroquinolones; D006801:Humans; D009643:Norfloxacin / Q000493:pharmacokinetics; D015242:Ofloxacin / Q000493:pharmacokinetics; D015363:Quinolones / Q000493:pharmacokinetics; D011817:Rabbits
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D015242:Ofloxacin; C053091:lomefloxacin; D009643:Norfloxacin