[Plasma prostaglandin E2 in pregnant women undergoing labor induction with endocervical gel application].
The authors describe an assay that quantify with precision the plasmatic prostaglandin E2 (PGE2). The aim of this study was to evaluate the effect of the gel formulation PGE2 endocervical in the induction of labour. We have studied 26 pregnant women without disease at the term of the pregnancy. The blood sample was collected before and 30 minutes after the labor induction in tubes with 10 mg EDTA Na2 and 5 microg of aspirin. The PGE2 was isolated by extraction and chromatography with 70-100% recovery. The concentration was evaluated by a completion assay with RIA. The PGE2 in plasma was found before and after the induction of labor with the following results: 347 +/- 51.6 and 719 +/- 128 pg/ml. The data was compared by the Student's t test for paired values (df = 50, t = 13. 22 and p < 0.0001). We can conclude that in pregnant women the concentration of PGE2 in plasma was double after the labor induction with the sensitivity in 1.25 pg/tube and the reproducibility 4.6% intraassay and 7% interassay showing that the method is sensitive, reproducible and efficient for the PGE2.
Siqueira
M
M
Centro de Gastroenterología de la Facultad de Medicina de Lisboa, Lisboa, Portugal.
Neves
J
J
Arteaga
M
M
Bicho
M
M
Silva Cruz
A
A
D000282:Administration, Intravaginal; D000293:Adolescent; D000328:Adult; D002584:Cervix Uteri; D015232:Dinoprostone / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D005782:Gels; D006801:Humans; D007751:Labor, Induced / Q000379:methods*; D010120:Oxytocics / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005782:Gels; D010120:Oxytocics; D015232:Dinoprostone