Cyclic expression of mRNA transcripts for connective tissue components in the mouse ovary.
In the ovary, differentiation of germinal cells into primordial follicles, functional ovulatory follicles and corpus luteum, all take place in a connective tissue matrix. We postulated that extracellular matrix (ECM) of the ovary participates actively in ovarian functions. To test this, the mRNA levels for several ECM components were determined in the mouse ovary at six distinct stages of the 4-day oestrous cycle. Northern analysis revealed statistically significant cyclic expression patterns for the mRNAs coding for type III, IV and VI collagens as well as for the small proteoglycan, biglycan, and for syndecan-1 and osteonectin. The cyclic changes observed in the mRNAs for these structural components exceeded those for matrix metalloproteinases (MMP)-2, -9 and -13, and for tissue inhibitors of matrix metalloproteinases (TIMP)-1, -2 and -3, where the changes were not statistically significant, despite their apparent role in ECM remodelling in the ovary. These observations support the hypothesis that cyclic changes in the production and degradation of ECM are part of normal ovarian function connected with follicular maturation, rupture and corpus luteum formation.
Oksjoki
S
S
Department of Molecular Biology and Medical Biochemistry, Turku University Central Hospital, University of Turku, Kiinamyllynkatu 4-6, FIN-20520 Turku, Finland.
Sallinen
S
S
Vuorio
E
E
Anttila
L
L
D000818:Animals; D058578:Biglycan; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics; D003094:Collagen / Q000235:genetics*; D003238:Connective Tissue / Q000737:chemistry / Q000502:physiology; D003239:Connective Tissue Cells / Q000502:physiology; D058575:Decorin; D004971:Estrus / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D000071235:Fibromodulin; D005786:Gene Expression Regulation; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D015676:Osteonectin / Q000235:genetics; D010053:Ovary / Q000166:cytology* / Q000502:physiology*; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D053668:Syndecan-1; D053667:Syndecans; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics; D014158:Transcription, Genetic
C546885:Bgn protein, mouse; D058578:Biglycan; D002352:Carrier Proteins; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; D008562:Membrane Glycoproteins; D015676:Osteonectin; D011509:Proteoglycans; D012333:RNA, Messenger; C508242:Sdc1 protein, mouse; D053668:Syndecan-1; D053667:Syndecans; D019715:Tissue Inhibitor of Metalloproteinase-1; D019717:Tissue Inhibitor of Metalloproteinase-3; D000071235:Fibromodulin; D019716:Tissue Inhibitor of Metalloproteinase-2; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9