Experimental studies on affinity chromatography in an electric field.
A multicompartment electrolyzer, which has been used for preparative electrophoresis [Z. Liu, Z. Huang, J.-Y. Cong, et al., Sep. Sci. Technol. 31 (1996) 427], is applied for carrying out affinity chromatography in an alternating electric field. The effect of electric field strength on the adsorption and desorption characteristics is experimentally examined with human serum albumin and Blue Sepharose Fast Flow as a model system. It is shown that the existence of an electric field leads to a significant change in the adsorption capacity of the blue dye, which may be used for establishing a preferential adsorption to achieve a high resolution. The adsorption speed increases slightly with respect to the increase of electric field strength, while adsorption capacity in the presence of an electric field is independent of the electric field strength. Different elution behavior is observed as function of adsorption condition and a high recovery of the adsorbed protein is obtained when the adsorption is carried out in the presence of an electric field.
Liu
Z
Z
Department of Chemical Engineering, Tsinghua University, Beijing, China. liuzheng@mail.tsinghua.edu.cn
Feng
S
S
Yin
J
J
Ding
F
F
Yuan
N
N
D002021:Buffers; D002846:Chromatography, Affinity / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012709:Serum Albumin / Q000737:chemistry
D002021:Buffers; D012709:Serum Albumin