[Age dependence of the density of alpha-1 adrenergic receptors in liver cell membranes of domestic fowl].
Age dependence of alpha 1-adrenergic receptor number on hepatocytes of chickens was determined by incubation of liver cell membranes from chickens of different ages with 3H-prazosin. We showed, that there is a continuous and significant rise of alpha 1-adrenergic binding sites with increasing age of the chickens from 226 +/- 22 fmol/mg protein at the age of up to 4 weeks to 628 +/- 34 fmol/mg protein in laying hens. Changes in receptor affinity could not be registered. The KD-values ranged insignificantly from 0.34 +/- 0.03 nM to 0.50 +/- 0.06 nM in the different ages of chickens and amounted to 0.44 +/- 0.02 nM averagely. The classification of the binding sites for 3H-prazosin to the alpha 1B-subtype could be caused by the high sensitivity to chlorethylclonidine and by the low affinity for alpha A-adrenergic antagonists.
Neubert
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E
Veterinär-Physiologisch-Chemisches Institut der Veterinärmedizinischen Fakultät der Universität Leipzig.
Gründel
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G
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D005260:Female; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D011224:Prazosin / Q000493:pharmacokinetics; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D014316:Tritium
D018340:Receptors, Adrenergic, alpha-1; D014316:Tritium; D011224:Prazosin