A novel biphenotypic B-cell precursor leukemia cell line (NALM-29) carrying t(9;22)(q34;q11) established from a patient with acute leukemia.
A novel biphenotypic leukemia cell line, NALM-29, was established from a 46-year-old Japanese male patient with acute lymphoblastic leukemia (ALL). The primary leukemic blasts showed a common ALL phenotype with CD19+, CD10+, CD13-, HLA-DR+ and Igs-. NALM-29 cells display biphenotypic characteristics: expression of the intracellular enzyme myeloperoxidase at the mRNA and protein level and cell surface positivity for CD19, CD10, CD13, CD33 and HLA-DR. NALM-29 fulfills EGIL criteria as B-cell precursor (BCP) leukemia B-II type. NALM-29 cells have a lymphoblastic morphological appearance; the immunoglobulin heavy chain gene is rearranged. NALM-29 cells responded significantly to the proliferative stimuli of FLT-3 ligand and IL-7, but not to GM-CSF, IL-3, IL-6, PIXY-321 or SCF. Proliferation of cells was inhibited significantly by IL-4, TNF-alpha or TNF-beta treatment. Cytogenetic analysis revealed the characteristic t(9;22)(q34;q11); expression of the m-bcr e1-a2 BCR-ABL fusion gene (typically found in ALL) was determined by PCR amplification of cDNA. The immunological, cytogenetic and functional characterization of NALM-29 suggests that this cell line may represent a scientifically significant in vitro model for BCP-type leukemia cells with biphenotypic characteristics.
Matsuo
Y
Y
Fujisaki Cell Center, Hayashibara Biochemical Labs Inc., Okayama, Japan. yomatsuo@hayashibara.co.jp
Drexler
H G
HG
Takeuchi
M
M
Orita
K
K
D001483:Base Sequence; D002455:Cell Division; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D016172:DNA Fingerprinting; D017931:DNA Primers; D006801:Humans; D007135:Immunoglobulin Variable Region; D016130:Immunophenotyping; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D018121:Receptors, Cytokine / Q000378:metabolism; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D007135:Immunoglobulin Variable Region; D018121:Receptors, Cytokine