[Study of mechanism of PTH modulating cytosolic free calcium in rat DCT].
OBJECTIVE
In order to understand the mechanism of PTH in regulating cytosolic free calcium concentration ([Ca2+]i), we differentiated rat renal proximal and distal convoluted tubules (PCT&DCT) by Percoll density gradient centrifugation, and successfully gauged the effect of hPTH (1-84), dibutyryl cAMP (db cAMP, a kind of cAMP activator) and prostaglandin E2 (PGE2) on [Ca2+]i of DCT using fluorescent Ca2+ indicator Fluo 3-AM. We found that PTH, db cAMP and PGE2 all could increase [Ca2+]i.


RESULTS
The results suggest that the rise in [Ca2+]i induced by PTH in DCT was through activation of cAMP, and PGE2 might be also involved in regulation of [Ca2+]i as a messenger of PTH.
Song
Y
Y
Institute of Basic Medical Sciences, CAMS, Beijing.
Cheng
J
J
Duan
J
J
Liu
J
J
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D005260:Female; D006801:Humans; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010281:Parathyroid Hormone; D003994:Bucladesine; D002118:Calcium