title: Regional distribution and localization of zinc and metallothionein in the intestine of rats fed diets differing in zinc content.
abstract: BACKGROUND
  Zinc (Zn) is protective and enhances epithelial repair in gut diseases. In this study we investigate the localization and distribution of Zn and its binding protein, metallothionein (MT), in the gut of rats fed diets varying in Zn content.
  
  
  METHODS
  Male-Sprague Dawley rats were fed low, normal, high, or excess Zn in their diets (10, 100, 400, or 1000 mg Zn/kg, respectively) and killed 7 days later. Blood, liver, and gut tissues were collected. Tissue Zn was determined with atomic absorption spectrophotometery and MT with a Cd/haem affinity assay. Zn and MT were immunohistochemically localized in the small-intestinal wall with zinquin and an anti-MT antibody.
  
  
  RESULTS
  Most Zn in the intestinal wall was present in the mucosal scrapings, with 94% membrane-bound and 6% cytosolic, irrespective of dietary Zn. MT levels increased in all gut regions at dietary Zn levels above 100 mg Zn/kg. MT was 40% higher in the ileum than in other gut regions in rats fed low- and normal-Zn diets. The Zn content of the ileum was also 20% higher than that of other gut regions in rats fed low-, normal-, or high-Zn diets. Zn and MT were colocalized in the base of the intestinal crypts, most visibly in the ileum.
  
  
  CONCLUSIONS
  Mucosal cytosolic Zn and MT concentrations are increased only at high or excessive Zn intakes in all gut regions except the ileum, which can respond to a lower Zn intake. As the cytosolic Zn pool most likely influences mucosal protection and repair mechanisms, it is proposed that an increased MT may indicate the adequacy of oral Zn therapy in gut disease.
authors.affiliation: Dept. of Physiology, The University of Adelaide, Institute of Medical and Veterinary Science, SA, Australia.
authors.forename: C D
authors.initials: CD
authors.lastname: Tran
authors.forename: R N
authors.initials: RN
authors.lastname: Butler
authors.forename: G S
authors.initials: GS
authors.lastname: Howarth
authors.forename: J C
authors.initials: JC
authors.lastname: Philcox
authors.forename: A M
authors.initials: AM
authors.lastname: Rofe
authors.forename: P
authors.initials: P
authors.lastname: Coyle
mesh_terms: D000009:Abdominal Muscles / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000032:analysis* / Q000276:immunology; D008856:Microscopy, Fluorescence; D015363:Quinolones; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D014105:Tosyl Compounds; D015032:Zinc / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics*
chemical_list: D005456:Fluorescent Dyes; D015363:Quinolones; D014105:Tosyl Compounds; D008668:Metallothionein; D015032:Zinc; C084731:zinquin