Isolation and characterization of cyclic alpha-(1-->4)-glucans having degrees of polymerization 9-31 and their quantitative analysis by high-performance anion-exchange chromatography with pulsed amperometric detection.
Cyclic alpha-(1-->4)-glucans with degrees of polymerization (DPs) 9-31 were isolated from a mixture of cyclization products formed in the early stage of the action of cyclodextrin glucanotransferase (CGTase) on synthetic amylose, and characterized by matrix-assisted laser desorption ionization time-of-flight MS, 13C-NMR and HPLC of their partial acid hydrolyzates. High-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection for an accurate estimation of cyclic alpha-(1-->4)-glucans was developed using those isolate glucans as quantification standards, and by HPAEC, the time course of the cyclization reaction of CGTase from an alkalophilic Bacillus sp. A2-5a on synthetic amylose was determined.
Koizumi
K
K
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Sanbe
H
H
Kubota
Y
Y
Terada
Y
Y
Takaha
T
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005936:Glucans; D011108:Polymers; C040088:1,4-glucan