Effect of suplatast tosilate (IPD-1151T) on cytokine production by allergen-specific human Th1 and Th2 cell lines.
Suplatast tosilate (IPD-1151T) is an antiallergic agent that suppresses airway eosinophil infiltration in asthma. We investigated the effects of IPD-1151T on proliferative response and cytokine production by human antigen-specific T cell lines. Purified protein derivatives (PPD)-specific T helper 1 (Th1) cell lines and Dermatophagoides farinae (Der f)-specific T helper 2 (Th2) cell lines were established from patients with asthma sensitized with house dust mite. Stimulation of PPD-specific and Der f-specific T cell lines with relevant antigens resulted in production of mostly interferon (IFN)-gamma and of interleukin (IL)-4 and IL-5, respectively. IPD-1151T did not inhibit the proliferative responses of either the Th1 or Th2 cell line to antigens. Although IPD-1151T did not inhibit IFN-gamma production by PPD-specific Th1 cell lines, it did inhibit IL-4 and IL-5 production by antigen-stimulated Der f-specific Th2 cell lines in a dose-dependent manner. IPD-1151T directly inhibited cytokine production by Der f-specific Th2 cell lines stimulated with immobilized anti-CD3 antibodies. Although IPD-1151T did not inhibit the clonal expansion of memory T cells among PBMCs into PPD-specific Th1 and Th2 cell lines, it did inhibit IL-4 and IL-5 production by Der f-specific Th2 cell lines but not IFN-gamma production by PPD-specific Th1 cell lines. These results suggest that IPD-1151T selectively inhibits Th2-type cytokine production.
Oda
N
N
First Department of Internal Medicine, Showa University, School of Medicine, Tokyo, Japan.
Minoguchi
K
K
Yokoe
T
T
Hashimoto
T
T
Wada
K
K
Miyamoto
M
M
Tanaka
A
A
Kohno
Y
Y
Adachi
M
M
D000485:Allergens / Q000276:immunology; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D039741:Antigens, Dermatophagoides; D001190:Arylsulfonates / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D000939:Epitopes / Q000187:drug effects; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008925:Mites / Q000276:immunology; D013452:Sulfonium Compounds / Q000494:pharmacology*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
D000485:Allergens; D018926:Anti-Allergic Agents; D039741:Antigens, Dermatophagoides; D001190:Arylsulfonates; D016207:Cytokines; D000939:Epitopes; D006023:Glycoproteins; D013452:Sulfonium Compounds; D007371:Interferon-gamma; C073028:suplatast tosilate