Effects of in vivo ischemia on contractile responses of rabbit bladder to field stimulation, carbachol, ATP and KCl.
Rabbits were subjected to bilateral ischemia for 3, 6, or 18 h then euthanized and their bladders excised. Isolated strips of rabbit bladder detrusor were incubated in normal Tyrode's solution and contractile responses to FS, carbachol, ATP, and KCl measured. Maximal contraction, maximal rate of tension generation, and length of time to maximal contraction were determined. These studies revealed that contractile responses to FS (neurogenic stimulation) were most affected by ischemia. Contractile responses to carbachol, ATP and KCl were all similarly sensitive to ischemia.
Bratslavsky
G
G
Division of Urology, Albany Medical College, Albany College of Pharmacy, Albany, NY 12208, USA.
Whitbeck
C
C
Horan
P
P
Levin
R M
RM
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D004558:Electric Stimulation; D007511:Ischemia / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D018722:Nicotinic Agonists / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology; D001743:Urinary Bladder / Q000098:blood supply / Q000187:drug effects / Q000502:physiology*
D018722:Nicotinic Agonists; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D002217:Carbachol