Fluorimetric determination of D- and L-lactate derivatized with 4-(N, N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) by high-performance liquid chromatography.
A highly sensitive method, based on fluorescence derivatization with 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazol e (DBD-PZ), was developed for the determination of D- and L-lactic acid. High-performance liquid chromatographic separation of the lactic acid derivative was achieved using an octadecylsilica column followed by enantiomeric separation on a phenylcarbamoylated beta-cyclodextrin chiral column. The separation factor for D, L-lactic acid derivatives was 1.30 using MeOH/H(2)O (80/20) as a mobile phase, and the detection limits were approximately 360 and 300 fmol on column for D- and L-lactic acid derivative, respectively. The proposed method was applied to determine D- and L-lactate in a wine and a lactic drink. Both D- and L-lactate could be determined simultaneously, with the precisions ranging from 3.50 to 5.78% for intra-day and 4.28-9.92% for inter-day determinations. The relative recovery was in the range of 91.6-100%.
Fukushima
T
T
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Hongo 7-3-1, Tokyo 113-0033, Japan.
Adachi
S
S
Ichihara
H
H
Al-Kindy
S
S
Imai
K
K
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D007773:Lactates / Q000032:analysis* / Q000737:chemistry; D010069:Oxadiazoles / Q000032:analysis* / Q000737:chemistry; D010879:Piperazines / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D014920:Wine / Q000032:analysis
D007773:Lactates; D010069:Oxadiazoles; D010879:Piperazines; D013449:Sulfonamides; C076762:4-(N,N-dimethylaminosulphonyl)-7-(1-piperazinyl)-2,1,3-benzoxadiazole