FASAY: a simple functional assay in yeast for identification of p53 mutation in tumors.
Alteration of the p53 tumor suppressor gene is the most common genetic defect known to occur in human tumors. Germ-line p53 mutations significantly increase the risk of developing diverse malignancies. FASAY is a simple functional assay for germ-line and somatic mutations in the p53 gene altering the transactivation capability of the p53 protein. The method was successfully used for mutation analysis of p53 in various cell lines, somatic tumor cells and blood cells. In addition, FASAY was also found effective as a tool for basic research of binding of mutant p53 proteins to promoters of different p53 target genes.
Smardová
J
J
Masaryk Memorial Cancer Institute, Brno, Czech Republic.
D004252:DNA Mutational Analysis / Q000379:methods*; D016158:Genes, p53; D018095:Germ-Line Mutation; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*