title: Genotyping for human platelet-specific antigens HPA-1, -2, -3, -4 and -5 in the Slovenian population reveals a slightly increased frequency of HPA-1b and HPA-2b as compared to other European populations.
abstract: Typing of human platelet alloantigens (HPA) is necessary in various clinical situations. The purpose of this study was to type a random sample of the Slovenian population for HPA alleles, in order to obtain genetic population data. A total of 152 unrelated Slovenian blood donors were genotyped for HPA-1, -2, -3, -4 and -5 alleles using a simple method that enables simultaneous and complete determination of HPA genotypes. Ten different polymerase chain reactions employing sequence-specific priming (PCR-SSP), which worked in identical cycling conditions, were used. The allele frequencies were 0.809 for HPA-1a, 0.191 for HPA-1b, 0.891 for HPA-2a, 0.109 for HPA-2b, 0.591 for HPA-3a, 0.407 for HPA-3b, 0.997 for HPA-4a, 0.00 for HPA-4b, 0.934 for HPA-5a and 0.066 for HPA-5b. When compared to results of studies of various other Caucasian populations, our population displayed a slightly but not significantly higher proportion of the HPA-1b and 2b alleles.
authors.affiliation: Blood Transfusion Centre of Slovenia, Ljubljana, Slovenia.
authors.forename: P
authors.initials: P
authors.lastname: Rozman
authors.forename: J
authors.initials: J
authors.lastname: Drabbels
authors.forename: R F
authors.initials: RF
authors.lastname: Schipper
authors.forename: I
authors.initials: I
authors.lastname: Doxiadis
authors.forename: S
authors.initials: S
authors.lastname: Stein
authors.forename: F H
authors.initials: FH
authors.lastname: Claas
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006720:Homozygote; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017524:Slovenia / Q000453:epidemiology
chemical_list: C071221:2b alloantigen, human; D016824:Antigens, Human Platelet; C083451:human platelet antigen 1b