title: Characterization and distribution of B cells in the lymphoid organs of goats.
abstract: The distribution of B cells in the lymphoid organs of the goat was studied using a panel of 13 monoclonal antibodies (mAbs) developed against different markers for bovine B cells. Samples of mesenteric lymph nodes, jejunal and ileal Peyer's patches, duodenum, jejunum, ileum, colon, caecum and rectum were taken from four 7-month-old male Murciano-granadina goats using the avidin-biotin-peroxidase (ABC) method on frozen sections as described by Hsu et al. (1981). The mAbs against immunoglobulins (Ig) recognized a large number of cells, particularly in the light zones of the germinative centres of the lymphoid follicles, regardless of the isotype against which they were directed. However, the greatest numbers of B cells in the germinative centres and outer coronas of the lymphoid follicles of the lymph node, spleen and Peyer's patches were recognized by mAbs against the L lambda chain of Ig and against IgM. This was also the case in other locations where B cells were abundant, such as the medulla of the lymph node and the dome of the Peyer's patches. These mAbs recognized not only B lymphocytes but also plasma cells, showing an intracytoplasmatic reaction (numerous in the spleen red pulp and the intestinal lamina propria when mAbs were used against the L lambda chain of the Ig, scarce in the intestinal lamina propria when used against IgM and scarce in spleen red pulp and numerous in the intestinal lamina propria when mAbs against IgA were used). The mAbs BAQ44 A, GC65 A and GB25 A are of interest because, besides marking cells in the B areas where lymphocytes show surface Ig, they give a positive reaction in areas where there are Ig-cells (the dark zone of the germinative centre) and do not immunostain plasma cells. Thus, these mAbs recognize a surface marker which is not an Ig.
authors.affiliation: U.D. Histology and Anatomic Pathology, Veterinary Faculty, University of Murcia, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Pallarés
authors.forename: J
authors.initials: J
authors.lastname: Seva
authors.forename: A
authors.initials: A
authors.lastname: Bernabé
authors.forename: M A
authors.initials: MA
authors.lastname: Gómez
authors.forename: J A
authors.initials: JA
authors.lastname: Navarro
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002417:Cattle; D006041:Goats / Q000033:anatomy & histology*; D007136:Immunoglobulins / Q000032:analysis; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D008297:Male; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D007136:Immunoglobulins