Role of HLA-G versus HLA-E on NK function: HLA-G is able to inhibit NK cytolysis by itself.
Recent studies have shown that endogenous HLA-E molecules are stabilized on the cell surface upon the expression of HLA-G which contains within its leader sequence, a nonapeptide capable of binding with the HLA-E/beta2m complex. Since HLA-E was found to be the major ligand for the CD94/NKG2A inhibitory receptor, we determined the role of HLA-G versus HLA-E on NK lysis inhibition. We showed that K562 cells transfected with HLA-G1 cDNA are protected from NK lysis by direct interaction between HLA-G1 and killing inhibitory receptor(s). This NK lysis inhibition is not dependent on HLA-E expression, since no HLA-E protein was detected on K562 cells; HLA-G1 is therefore able to inhibit NK lysis by itself.
Khalil-Daher
I
I
Service de Recherches en Hémato-Immunologie, Commissariat à l'Energie Atomique-DRM-DSV, Hôpital Saint-Louis, Paris, France.
Riteau
B
B
Menier
C
C
Sedlik
C
C
Paul
P
P
Dausset
J
J
Carosella
E D
ED
Rouas-Freiss
N
N
D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology*; D000097625:HLA-E Antigens
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens