[MA891--an established spontaneous mouse mammary adenocarcinoma cell line and its immunologic characteristics].
OBJECTIVE
To characterize the immunologic properties of an established mouse mammary tumor cell line MA891.


METHODS
From a spontaneous mouse mammary adenocarcionma (TA2 MA891) of TA2 mouse origin, an in vitro passaged cell line MA891 was maintained in RPMI1640 with 10% new born calf serum. The immunogenecity of MA891 was studied by classical tumor transplantation rejection assay, generation of cytotoxic T lymphocytes (CTL) by syngeneic secondary mixed lymphocyte-tumor cell culture (MLTC) and cytotoxic assay of tumor infiltrating lymphocytes (TIL).


RESULTS
MA891 maintained the high metastatic potential of the parental TA2 MA891 tumor passaged in vivo. Transplantation rejection studies indicated that amputation of a hind limb with growing tumor in the footpad did not protect the mice from a second subcutaneous challenge of the same tumor. The cytotoxic activities of both CTL and TIL were shown to be very weak.


CONCLUSIONS
MA891 is a mouse tumor of very low immunogenecity. It can be used as a mouse tumor model for studying cancer metastasis in humans.
Zhou
Y
Y
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Luo
L
L
Liu
X
X
Gao
P
P
Su
Y
Y
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology*; D000818:Animals; D005260:Female; D008175:Lung Neoplasms / Q000556:secondary; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured