Measuring respiration of cultured cell with oxygen electrode as a metabolic indicator for drug screening.
New trend in methods for assessing pharmacological action to bacteria and cell is to measure their metabolic activities induced, while the conventional methods used population growth. We focused on respiration volume as an indicator of cell metabolism, and developed inexpensive disposable oxygen electrode sensor and multi-channel dissolved oxygen meters (DOX-10 and DOX-96KB). Using these instruments, cytotoxicity was measured for 48 hrs and the method showed superior features to conventional methods in its handiness of one step assay, and excellent adaptability to automated systems. Total usability of this oxygen electrode method is being evaluated in bacterial drug susceptibility test, anticancer drug susceptibility test, and alternatives to animal experiment.
Amano
Y
Y
Daikin Industries, Ltd.
Okumura
C
C
Yoshida
M
M
Katayama
H
H
Unten
S
S
Arai
J
J
Tagawa
T
T
Hoshina
S
S
Hashimoto
H
H
Ishikawa
H
H
D000970:Antineoplastic Agents / Q000494:pharmacology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004566:Electrodes; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D010100:Oxygen; D010101:Oxygen Consumption / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000970:Antineoplastic Agents; D010100:Oxygen