title: Degradation of lignite (low-rank coal) by ligninolytic basidiomycetes and their manganese peroxidase system
abstract: Ligninolytic basidiomycetes (wood and leaf-litter-decaying fungi) have the ability to degrade low-rank coal (lignite). Extracellular manganese peroxidase is the crucial enzyme in the depolymerization process of both coal-derived humic substances and native coal. The depolymerization of coal by Mn peroxidase is catalysed via chelated Mn(III) acting as a diffusible mediator with a high redox potential and can be enhanced in the presence of additional mediating agents (e.g. glutathione). The depolymerization process results in the formation of a complex mixture of lower-molecular-mass fulvic-acid-like compounds. Experiments using a synthetic 14C-labeled humic acid demonstrated that the Mn peroxidase-catalyzed depolymerization of humic substances was accompanied by a substantial release of carbon dioxide (17%-50% of the initially added radio-activity was released as 14CO2). Mn peroxidase was found to be a highly stable enzyme that remained active for several weeks under reaction conditions in a liquid reaction mixture and even persisted in sterile and native soil from an opencast mining area for some days.
authors.affiliation: Institut fur Technische Mikrobiologie, Friedrich-Schiller-Universitat Jena, Germany.
authors.initials: M
authors.lastname: Hofrichter
authors.initials: D
authors.lastname: Ziegenhagen
authors.initials: S
authors.lastname: Sorge
authors.initials: R
authors.lastname: Ullrich
authors.initials: F
authors.lastname: Bublitz
authors.initials: W
authors.lastname: Fritsche