Determination of carbohydrate-deficient transferrin separated by lectin affinity chromatography for detecting chronic alcohol abuse.
Carbohydrate-deficient transferrin (CDT) has been established as a valuable biological marker for detecting chronic alcohol abuse. To improve the diagnostic efficiency, we studied new CDT determination procedures involving the use of lectin affinity chromatography with Allomyrina dichotoma agglutinin (allo A) and Trichosanthes japonica agglutinin I (TJA-I) to isolate the CDT isoforms CDT-allo A and CDT-TJA, respectively. These procedures, based on detection of the CDT-allo A and CDT-TJA isoforms in sera, showed high sensitivity (100% and 98%, respectively) and high specificity (93% and 85%, respectively). These results demonstrate that the new procedures involving the use of lectin affinity chromatography are more useful for isolating markers in the CDT test than the conventional charge-based separation method.
Yoshikawa
K
K
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
Umetsu
K
K
Shinzawa
H
H
Yuasa
I
I
Maruyama
K
K
Ohkura
T
T
Yamashita
K
K
Suzuki
T
T
D000437:Alcoholism / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods / Q000592:standards / Q000706:statistics & numerical data; D018981:Congenital Disorders of Glycosylation / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000592:standards; D010641:Phenotype; D020033:Protein Isoforms / Q000302:isolation & purification / Q000592:standards; D012016:Reference Values; D018709:Statistics, Nonparametric; D014168:Transferrin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism
C045794:Allomyrina lectin; D015415:Biomarkers; D037102:Lectins; D020033:Protein Isoforms; D014168:Transferrin; C056759:carbohydrate-deficient transferrin