Study of a rat skin in vivo micronucleus test: data generated by mitomycin C and methyl methanesulfonate.
We have developed an in vivo micronucleus (MN) test that uses rat skin as the target organ. Sample preparation involves cold-treating the epidermis with trypsin, peeling it off with a fine forceps, treating it in hypotonic solution, and staining it with acridine orange (A.O.). We evaluated the assay using mitomycin C (MMC) and methyl methanesulfonate (MMS) as model clastogens, applying them as single and repeat treatments. Both chemicals induced a significant, dose-dependent increase in MN frequency in basal cells. One treatment per day for 3 days was optimal for MN induction.
Nishikawa
T
T
Human Safety Evaluation Center, Lion, 100 Tajima, Odawara-shi, Kanagawa 256-0811, Japan. ntaka@lion.co.jp
Haresaku
M
M
Adachi
K
K
Masuda
M
M
Hayashi
M
M
D000165:Acridine Orange; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D008297:Male; D008741:Methyl Methanesulfonate / Q000008:administration & dosage / Q000633:toxicity*; D015162:Micronucleus Tests / Q000379:methods*; D016685:Mitomycin / Q000008:administration & dosage / Q000633:toxicity*; D008954:Models, Biological; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects*
D005456:Fluorescent Dyes; D009153:Mutagens; D016685:Mitomycin; D008741:Methyl Methanesulfonate; D000165:Acridine Orange