title: Role of individual surface charges of voltage-gated K channels.
abstract: Fixed charges on the extracellular surface of voltage-gated ion channels influence the gating. In previous studies of cloned voltage-gated K channels, we found evidence that the functional surface charges are located on the peptide loop between the fifth transmembrane segment and the pore region (the S5-P loop). In the present study, we determine the role of individual charges of the S5-P loop by correlating primary structure with experimentally calculated surface potentials of the previously investigated channels. The results suggest that contributions to the surface potential at the voltage sensor of the different residues varies in an oscillating pattern, with the first residue of the N-terminal end of the S5-P loop, an absolutely conserved glutamate, contributing most. An analysis yields estimates of the distance between the residues and the voltage sensor, the first N-terminal residue being located at a distance of 5-6 A. To explain the results, a structural hypothesis, comprising an alpha-helical N-terminal end of the S5-P loop, is presented.
authors.affiliation: The Nobel Institute for Neurophysiology, Department of Neuroscience, Karolinska Institutet, S-171 77 Stockholm, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Elinder
authors.forename: P
authors.initials: P
authors.lastname: Arhem
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology; D008433:Mathematics; D008564:Membrane Potentials / Q000502:physiology*; D008954:Models, Biological; D015221:Potassium Channels / Q000737:chemistry* / Q000502:physiology*; D017433:Protein Structure, Secondary; D015203:Reproducibility of Results
chemical_list: D001426:Bacterial Proteins; D015221:Potassium Channels; C107790:prokaryotic potassium channel