Cellular localization of estrogen receptors on neurones in various regions of cultured rat CNS: coexistence with cholinergic and galanin receptors.
Autoradiographic studies have shown that many neurones in explant cultures of rat neocortex, hippocampus, preoptic area and spinal cord express binding sites for [3H]-estradiol which are distributed over the cell bodies and primary processes. By means of immunohistochemistry, it was observed that neurones were labelled by monoclonal antibodies against estrogen alpha-receptors and a polyclonal antibody against estrogen beta-receptors. Immunoreactivity was distributed over the soma and primary processes of the cells, the nuclei being more intensely stained. Double-immunostaining revealed a colocalization of estrogen alpha- and beta-receptors on approximately half of the neurones in cultures from neocortex and hippocampus whereas in cultures from preoptic area and spinal cord only few cells were double-stained. On many neurones, a coexistence of estrogen receptors and cholinergic muscarinic or nicotinic sites was found. Furthermore, combined autoradiographic and immunohistochemical studies have shown a colocalization of receptors for estrogen and the neuropeptide [125I]-galanin. The coexistence of estrogen and cholinergic sites as well as of estrogen and galanin receptors on the same neurones are discussed with respect to neurodegenerative events such as Alzheimer's disease.
Hösli
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Department of Physiology, University of Basel, Switzerland.
Hösli
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L
D000818:Animals; D001345:Autoradiography; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism*; D046508:Culture Techniques; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D044088:Receptors, Galanin; D011976:Receptors, Muscarinic / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism; D014018:Tissue Distribution
D020033:Protein Isoforms; D011950:Receptors, Cholinergic; D011960:Receptors, Estrogen; D044088:Receptors, Galanin; D011976:Receptors, Muscarinic; D018013:Receptors, Neuropeptide; D011978:Receptors, Nicotinic; D004958:Estradiol