title: Association of the rat heterogeneous nuclear RNA-ribonucleoprotein F with TATA-binding protein.
abstract: Heterogeneous nuclear ribonucleoprotein F (hnRNP-F) has been shown to be a pre-mRNA splicing factor. Recent studies have uncovered the coordination of synthesis of pre-mRNA and its processing, including post-transcriptional modification and splicing. Here, we present evidence for an association between a splicing factor, hnRNP-F, and TATA-binding protein (TBP), which is an essential factor needed for transcription initiation. An affinity detection experiment revealed hnRNP-F in the preparation of TBP-interacting proteins. HnRNP-F was associated with TBP in nuclear extracts and was capable of direct binding to TBP in vitro. These results suggest that hnRNP-F is associated with TBP in the cell. HnRNP-F was observed in abundance in the thymus, spleen and testis, and its distribution pattern was similar to that of TBP, implying a functional coordination of transcription and splicing. We assume that the splicing machinery is associated with the transcription apparatus as a prerequisite prior to transcriptional elongation.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Tamura
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008969:Molecular Sequence Data; D015138:RNA, Nuclear / Q000378:metabolism*; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D015138:RNA, Nuclear; D012261:Ribonucleoproteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors