AFLPs represent highly repetitive sequences in Asparagus officinalis L.
The chromosomal and genomic organization of 5 cloned AFLP fragments in asparagus (Asparagus officinalis L.) were investigated. Two of the 5 AFLP loci were sex-linked. The fragments, amplified with EcoRI/Mse I primers, ranged from 107 to 267 bp and were AT-rich. Southern hybridization gave interspersed, middle repetitive to high copy sequence signals. Fluorescence in-situ hybridization (FISH) exhibited hybridization signals on all chromosomes with dispersed distribution pattern and varying signal intensities. Repetitive signals in the form of clusters were observed on all chromosomes. In addition, the 5S rRNA gene was physically mapped on one pair of chromosomes and the 18S-5.8S-25S rRNA genes on three pairs. The results of the FISH and Southern analyses showed that the AFLP marker technology relies on repetitive sequences. Since repetitive DNA sequences represent a fraction of the plant genome undergoing rapid changes during the course of evolution, the question of whether such molecular markers originating from repetitive DNA sequences remain stable is discussed.
Reamon-Büttner
S M
SM
Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Germany.
Schmidt
T
T
Jung
C
C
D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence; D019668:Liliaceae / Q000235:genetics*; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic; D012335:RNA, Ribosomal / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D012335:RNA, Ribosomal