Activation of spleen lymphocytes by plasmid DNA.
It was shown that plasmid pUC19 DNA stimulates in vitro proliferation of CBA mouse splenocytes in a dose-dependent manner. Stimulation effect of the plasmid DNA is additive with COn A or LPS, synergistic with PMA and is inhibited by nonimmunogenic phosphodiester oligonucleotides and Fab fragments of antimouse Ig antibodies. These data and the data of affinity labelling of ODN-binding proteins indicate that immunoglobulin receptors are involved in DNA-induced lymphocyte activation.
Rykova
E
E
Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Laktionov
P
P
Vlassov
V
V
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D004247:DNA