The induction of smooth muscle alpha actin in a transformed rat cell line suppresses malignant properties in vitro and in vivo.
A normal rat fibroblast 3Y1 cell line expresses smooth muscle a actin and the expression of alpha actin is suppressed in the transformant (SR-3Y1-2) induced by a Raus sarcoma virus. Gene transfer with smooth muscle alpha actin into the SR-3Y1-2 cell line reduced growth and invasiveness in vitro, as well as tumor growth and experimental lung metastasis depending on the expression of the alpha actin. These results indicated that smooth muscle alpha actin is involved in the regulation of cell growth as well as cell motility and thus leads to the suppression of malignant phenotypes in transformed cells.
Okamoto-Inoue
M
M
Department of Dermatology, Kurume University School of Medicine, Japan.
Kamada
S
S
Kimura
G
G
Taniguchi
S
S
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002455:Cell Division; D002461:Cell Line, Transformed; D002465:Cell Movement; D005260:Female; D005455:Fluorescent Antibody Technique; D018014:Gene Transfer Techniques; D019042:Muscle Neoplasms / Q000378:metabolism / Q000473:pathology; D009362:Neoplasm Metastasis; D010641:Phenotype; D011120:Polyomavirus; D051381:Rats; D013848:Thigh; D014407:Tumor Cells, Cultured
D000199:Actins