title: Activation of store-operated Ca2+ current in Xenopus oocytes requires SNAP-25 but not a diffusible messenger.
abstract: Depletion of Ca2+ stores in Xenopus oocytes activated entry of Ca2+ across the plasma membrane, which was measured as a current I(soc) in subsequently formed cell-attached patches. I(soc) survived excision into inside-out configuration. If cell-attached patches were formed before store depletion, I(soc) was activated outside but not inside the patches. I(soc) was potentiated by microinjection of Clostridium C3 transferase, which inhibits Rho GTPase, whereas I(soc) was inhibited by expression of wild-type or constitutively active Rho. Activation of I(soc) was also inhibited by botulinum neurotoxin A and dominant-negative mutants of SNAP-25 but was unaffected by brefeldin A. These results suggest that oocyte I(soc) is dependent not on aqueous diffusible messengers but on SNAP-25, probably via exocytosis of membrane channels or regulatory molecules.
authors.affiliation: Department of Pharmacology, University of California, San Diego, La Jolla 92093-0647, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.forename: A V
authors.initials: AV
authors.lastname: Ferrer-Montiel
authors.forename: M
authors.initials: M
authors.lastname: Montal
authors.forename: R Y
authors.initials: RY
authors.lastname: Tsien
mesh_terms: D036002:ADP Ribose Transferases / Q000494:pharmacology; D000818:Animals; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D020013:Calcium Signaling; D004058:Diffusion; D005089:Exocytosis; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D017136:Ion Transport; D008565:Membrane Proteins; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques; D050825:Synaptosomal-Associated Protein 25; D014982:Xenopus laevis; D020741:rho GTP-Binding Proteins
chemical_list: D015220:Calcium Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050825:Synaptosomal-Associated Protein 25; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins; D002118:Calcium