title: Triterpene antioxidants from ganoderma lucidum.
abstract: Ganoderma lucidum was studied for its antioxidative activity by bioassay guided isolation in conjunction with in vitro tests. The powdered crude drug was treated with boiling water and the aqueous extract (Ex1) was further separated to obtain terpene and polysaccharide fractions. The two fractions and Ex1 were screened for their antioxidative effect against pyrogallol induced erythrocyte membrane oxidation and Fe (II)-ascorbic acid induced lipid peroxidation. All tested samples showed antioxidative activities in a dose dependent manner and the terpene fraction was found to possess the highest effect compared with the others. Chemical isolation of the terpene fraction resulted in the detection of ganoderic acids A, B, C and D, lucidenic acid B and ganodermanontriol as major ingredients.
authors.affiliation: Department of Pharmacy, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. minzhu@cuhk.edu.hk
authors.forename: M
authors.initials: M
authors.lastname: Zhu
authors.forename: Q
authors.initials: Q
authors.lastname: Chang
authors.forename: L K
authors.initials: LK
authors.lastname: Wong
authors.forename: F S
authors.initials: FS
authors.lastname: Chong
authors.forename: R C
authors.initials: RC
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005296:Ferrous Compounds / Q000494:pharmacology; D006723:Hong Kong; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011748:Pyrogallol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020940:Reishi; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005296:Ferrous Compounds; D010936:Plant Extracts; D014315:Triterpenes; C051140:iron(II)-ascorbic acid complex; D011748:Pyrogallol; D001205:Ascorbic Acid