Lymphocyte stimulation test with drug-photomodified cells in patients with quinolone photosensitivity.
Quinolone antibacterial agents, known to elicit photosensitive dermatitis as an adverse effect, have both phototoxicity and photoallergenicity. The latter potency is mainly derived from their photohaptenic moiety; quinolones covalently bind to protein and cells upon exposure to ultraviolet A (UVA) light. Our previous study has shown the in vivo and in vitro antigenicity of quinolone-photomodified cells in mice. Here, we examined the presence of sensitized lymphocytes that react with quinolone-photomodified autologous cells in patients with photosensitivity to quinolones. A flow cytometric analysis using a monoclonal antibody specific to quinolone photoadducts demonstrated that peripheral blood mononuclear cells (PBMC) were successfully photomodified with quinolones upon exposure to UVA. PBMC from quinolone-photosensitive patients were cocultured with autologous PBMC photomodified with the causative drug. Modest but significant proliferative responses of responder lymphocytes were found in patients photosensitive to lomefloxacin, fleroxacin, and enoxacin, indicating photoallergic mechanism in these patients. On the other hand, sparfloxacin-photosensitive patients exhibited negative lymphocyte stimulation test, suggesting that its photosensitivity is mainly phototoxic. When UVA-preirradiated quinolones were used as stimulators, only fleroxacin exceptionally stimulated patients' PBMC, indicating its prohaptenic as well as photohaptenic properties. These findings suggest the presence of circulating sensitized T cells in patients with photosensitivity to certain quinolones.
Tokura
Y
Y
Department of Dermatology, Hamamatsu University School of Medicine, Japan. tokuray@hama-med.ac.jp
Seo
N
N
Ohshima
A
A
Yagi
H
H
Furukawa
F
F
Takigawa
M
M
D000368:Aged; D000369:Aged, 80 and over; D000890:Anti-Infective Agents / Q000009:adverse effects* / Q000097:blood; D017454:Dermatitis, Photoallergic / Q000097:blood / Q000209:etiology* / Q000276:immunology*; D017484:Dermatitis, Phototoxic / Q000097:blood / Q000209:etiology / Q000276:immunology; D004342:Drug Hypersensitivity / Q000097:blood / Q000209:etiology / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D024841:Fluoroquinolones; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000890:Anti-Infective Agents; D024841:Fluoroquinolones