Inhibitory effect of povidone-iodine for the antigen expression of human cytomegalovirus.
Anti-human cytomegalovirus (HCMV) properties of povidone-iodine (PVP-I) were evaluated in vitro. The effect of PVP-I was evaluated by indirect immunofluorescence assay on HCMV-infected MRC-5 cells. Percentages of fluorescent cells positive for HCMV immediate early and early antigens in cultures inoculated with AD 169 treated with PVP-I at various concentrations and reaction times were compared with the number of fluorescent cells in the controls inoculated with the virus alone. PVP-I was found to exert some inhibitory effect at a concentration of 0.5% and complete inhibition at a concentration of 7.5% on the infection of MRC-5 cells by HCMV AD 169 strain. Entirely new approaches to the prevention of HCMV infections in infants are necessary. The adequate use of PVP-I as a disinfectant may reduce the transmission of HCMV.
Numazaki
K
K
Department of Pediatrics, Sapporo Medical University, School of Medicine, Japan. numazaki@sapmed.ac.jp
Asanuma
H
H
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D000956:Antigens, Viral / Q000096:biosynthesis*; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000517:prevention & control*; D004622:Embryo, Mammalian; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008168:Lung / Q000166:cytology / Q000821:virology; D011206:Povidone-Iodine / Q000494:pharmacology*
D000891:Anti-Infective Agents, Local; D000956:Antigens, Viral; D000998:Antiviral Agents; D011206:Povidone-Iodine