title: Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro.
abstract: Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness that was comparable to that of strain TML. One isolate (GM3) was apparently substantially less invasive; electron microscopy showed this strain to be histotoxic - the probable reason for its reduced recovery from ileal mucosa and thus apparent 'low' invasiveness. Salmonella serotype Choleraesuis strain A50, isolated from a case of systemic salmonellosis in pigs, and serotype Dublin strain 3246, isolated from a case of systemic salmonellosis in calves, were also examined. Dublin strain 3246, when grown at 37 degrees C and used immediately in the invasion assay, damaged the mucosa in a manner similar to that of Typhimurium strain GM3, whereas Dublin strain 3246 grown at 37 degrees C and stored overnight at 4 degrees C did not. This was reflected in an apparently lower invasiveness of freshly grown organisms compared with that of organisms stored at 4 degrees C. In contrast, the histotoxicity of Typhimurium strain GM3 was not affected by storage at 4 degrees C. When stored at 4 degrees C, the levels of invasiveness of Choleraesuis strain A50 and Dublin strain 3246 were not significantly different from each other or from Typhimurium strain TML.
authors.affiliation: Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences.
authors.forename: Alex J
authors.initials: AJ
authors.lastname: Bolton
authors.affiliation: Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences.
authors.forename: GIllian D
authors.initials: GD
authors.lastname: Martin
authors.affiliation: Department of Physiology, Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Osborne
authors.affiliation: Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN.
authors.forename: Tim S
authors.initials: TS
authors.lastname: Wallis
authors.affiliation: Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences.
authors.forename: John
authors.initials: J
authors.lastname: Stephen
mesh_terms: D000818:Animals; D003080:Cold Temperature; D006801:Humans; D007082:Ileum / Q000382:microbiology* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000382:microbiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques / Q000379:methods; D011817:Rabbits; D012475:Salmonella / Q000145:classification / Q000276:immunology / Q000472:pathogenicity*; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification / Q000472:pathogenicity