Cisplatin-resistant derivatives of murine L1210 leukemia cells are not susceptible to growth-inhibiting and apoptosis-inducing actions of transforming growth factor-beta1.
Murine L1210 leukemia cells possessing an increased resistance to cisplatin were found to be refractory to transforming growth factor (TGF)-beta1-induced growth inhibition, while the parental L1210 cells were strongly inhibited by this cytokine. Growth inhibition was estimated on the basis of [3H]thymidine incorporation, cell counting and colony-forming assay. Cisplatin-resistant L1210 cells were also shown to be much more resistant than the parental cells to both cisplatin- and TGF-beta1-induced apoptosis. These results suggest the existence of cross-resistance to cisplatin and TGF-beta1 in the studied leukemia cells.
Stoika
R S
RS
Division of Regulatory Cell Systems, Institute of Biochemistry, National Academy of Sciences of Ukraine, Lviv. stoika@biochem.lviv.ua
Yakymovych MYa
Yakymovych
I A
IA
Chekhun
V F
VF
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D007939:Leukemia L1210 / Q000473:pathology / Q000628:therapy*; D051379:Mice; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D000970:Antineoplastic Agents; D016212:Transforming Growth Factor beta; D002945:Cisplatin