[beta-Endorphin enhances IL-2 and IFN-gamma gene expression in human blood mononuclear cells].
The effect of beta-endorphin (beta-END) stimulation of cytokines production by PHA-induced human peripheral blood mononuclear cells (PBMC) was studied by reverse transcription in combination with polymerase chain reaction (RT/PCR), slot blotting and hybridization to detect and measure messenger RNA (mRNA). beta-END significantly and dose dependently enhanced IFN-gamma and IL-2 mRNA expression of T cells and naloxone (Nal) reversed the effect of beta-END on IL-2 mRNA expression. The results further demonstrate the important link between the neural system and the immune system.
Lin
J
J
Institute of Basic Medical Sciences, CAMS, Beijing.
Shen
Y
Y
Gao
Y
Y
Li
L
L
D004305:Dose-Response Relationship, Drug; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D001615:beta-Endorphin / Q000494:pharmacology*
D007376:Interleukin-2; D012333:RNA, Messenger; D009270:Naloxone; D001615:beta-Endorphin; D007371:Interferon-gamma