Granulocyte and plasma cytokine activity in acute cadmium intoxication in rats.
Changes in the number and ex vivo function of peripheral blood neutrophils were investigated following intraperitoneal administration of cadmium-chloride in rats. Besides a dose-dependent increase in the number of peripheral blood neutrophils, changes were found in the functional state of isolated polymorphonuclear leukocytes (PMNs). Increased spontaneous adhesion and activation, and TNF activity in a conditioned medium were observed in cultures of granulocytes in comparison to granulocytes from control (saline-treated) animals. Increased levels of plasma activity of inflammatory cytokines, tumor necrosis factor (TNF) and interleukin-6 (IL-6) were noted following cadmium administration. Cytological signs of pulmonary inflammation were revealed histologically and the majority of neutrophils recovered from the lungs by enzyme digestion exhibited a capacity of nitroblue tetrazolium (NBT) reduction. Our data demonstrate that acute cadmium intoxication leads to a systemic inflammatory response characterized by numerical and functional changes in the granulocyte compartment and to increased levels of inflammation-related cytokine activity in the circulation. Correlations between the increased number of peripheral blood neutrophils and IL-6 plasma activity (r=0.776, p<0.00001) and the number of neutrophils recovered from the lung tissue (r=0.893, p<0.00001) suggested that systemic cadmium-induced inflammation might be involved in the pulmonary toxicity of cadmium.
Kataranovski
M
M
Institute for Medical Research, Military Medical Academy, Belgrade, Yugoslavia.
Kataranovski
D
D
Savic
D
D
Jovcic
G
G
Bogdanovic
Z
Z
Jovanovic
T
T
D000208:Acute Disease; D000818:Animals; D001772:Blood Cell Count; D002104:Cadmium / Q000008:administration & dosage; D002105:Cadmium Poisoning / Q000097:blood* / Q000473:pathology; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D015850:Interleukin-6 / Q000097:blood*; D008168:Lung / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D002104:Cadmium