title: Genetics and specific immune response in allergy to birch pollen and food: evidence of a strong, positive association between atopy and the HLA class II allele HLA-DR7.
abstract: BACKGROUND
  In some geographic areas birch pollen represents the most prominent cause for airborne allergic diseases. Up to 70% of patients allergic to birch pollen are hypersensitive to fruits, especially apples. Associations have been found, in some instances, with a sensitivity to aeroallergens and HLA class II genes.
  
  
  OBJECTIVE
  We investigated whether susceptibility or resistance to birch pollen allergy with and without food allergy was associated with HLA class II genes.
  
  
  METHODS
  Blood samples were obtained from 2 groups of unrelated European-born white adults: 42 atopic patients (31 of them with asthma) and 42 healthy control subjects with no personal or familial history of asthma or atopy. Their antibody responses to birch pollen, apples, grass, and weed pollens were evaluated by skin tests, RASTs, and immunoprints. Genomic DNA was extracted from PBLs. The exons of DQA1, DQB1, DRB1, and DPB1 genes were selectively amplified by using the PCR method. Genotyping was carried out by digestion of the amplified DNA products with allele-specific endonucleases (PCR-RFLP), which recognize allelic variations in the polymorphic exon.
  
  
  RESULTS
  We found no significant differences in the frequency of DPB1 alleles between patients and control subjects. HLA class II DR4 and/or DR7 alleles were present in 42.6% of the patients and in only 2.4% of the healthy subjects. These results confirm a previous study of a group of polysensitized atopic patients, which showed that DR4 and DR7 alleles were rare in healthy control subjects and frequently observed in atopic subjects with or without concomitant asthma.
  
  
  CONCLUSIONS
  We conclude that the allele HLA-DR7 is significantly involved in the presentation of apple and pollen allergens. However, we suggest that this susceptibility is more related to atopy than to specific responses to allergens.
authors.affiliation: Laboratoire d'Immuno-Allergie, Institut Pasteur, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Sénéchal
authors.forename: S
authors.initials: S
authors.lastname: Geny
authors.forename: F X
authors.initials: FX
authors.lastname: Desvaux
authors.forename: M
authors.initials: M
authors.lastname: Busson
authors.forename: C
authors.initials: C
authors.lastname: Mayer
authors.forename: Y
authors.initials: Y
authors.lastname: Aron
authors.forename: J P
authors.initials: JP
authors.lastname: Oster
authors.forename: J C
authors.initials: JC
authors.lastname: Bessot
authors.forename: G
authors.initials: G
authors.lastname: Peltre
authors.forename: G
authors.initials: G
authors.lastname: Pauli
authors.forename: E
authors.initials: E
authors.lastname: Swierczewski
mesh_terms: D000328:Adult; D000483:Alleles; D000485:Allergens; D000917:Antibody Formation; D052179:Antigens, Plant; D005260:Female; D005512:Food Hypersensitivity / Q000235:genetics* / Q000276:immunology*; D020022:Genetic Predisposition to Disease; D015809:HLA-DR7 Antigen / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics; D008297:Male; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics / Q000276:immunology*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D015809:HLA-DR7 Antigen; D000949:Histocompatibility Antigens Class II; D010940:Plant Proteins; C061312:Bet v 1 allergen, Betula