title: Characterization of the gltF gene product of Escherichia coli.
abstract: The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A gltF::Km(R) insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism.
authors.affiliation: Max-von-Pettenkofer-Institut für Hygiene und Mikrobiologie, Pettenkoferstr. 9a, D-80336, München, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Grassl
authors.forename: B
authors.initials: B
authors.lastname: Bufe
authors.forename: B
authors.initials: B
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Rösel
authors.forename: D
authors.initials: D
authors.lastname: Kleiner
mesh_terms: D020856:AT Rich Sequence; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins; D005838:Genotype; D008969:Molecular Sequence Data; D009876:Operon; D033903:Periplasmic Proteins; D010641:Phenotype; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D021382:Protein Sorting Signals; C078319:gltF protein, E coli