Induction of myelin gene expression in Schwann cell cultures by an interleukin-6 receptor-interleukin-6 chimera.
Expression of myelin basic protein (MBP) and Po gene products is induced during the final postnatal maturation of Schwann cells and reinduced during nerve regeneration. We show that a chimeric protein containing interleukin-6 fused to its soluble receptor (IL6RIL6 chimera) induces MBP and Po RNAs and proteins in cultures of dorsal root ganglia (DRG) from 14 day old mouse embryos. Activation of gp130 signaling by IL6RIL6 appears comparable to cyclic AMP elevating agents to induce the myelin gene products in DRG and in pure Schwann cell cultures.
Haggiag
S
S
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Chebath
J
J
Revel
M
M
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D004676:Myelin Basic Protein / Q000096:biosynthesis* / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis; D019947:Receptors, Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000096:biosynthesis; D012583:Schwann Cells / Q000378:metabolism*
D015850:Interleukin-6; D004676:Myelin Basic Protein; D015800:Protozoan Proteins; D019947:Receptors, Interleukin-6; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; C084989:ribosomal PO protein, Trypanosoma cruzi