title: Local and distant transfection of mdx muscle fibers with dystrophin and LacZ genes delivered in vivo by synthetic microspheres.
abstract: Patterns of dystrophin and beta-galactosidase expression were examined in mdx mice after i.m. injections of synthetic microspheres (MF-2) loaded with full-length (pHSADy) or mini-dystrophin gene (pSG5dys) cDNA plasmid constructs or with LacZ marker gene (pCMV-LacZ). A single injection of 25 microg pHSADy into quadriceps femoris muscle resulted in 6.8% of dystrophin positive myofibers (DPM) in a given muscle; 8.4% of DPM in glutaeus muscle and 4.3% of DPM in quadriceps femoris muscle of contralateral limb on day 21 after exposure compared with only 0.6% DPM in intact (non-injected) mdx mice. A high proportion of DPM (17.6% and 10.8%, respectively) was registered in both injected and contralateral muscles after mini- gene cDNA administration. MF-2/dystrophin cDNA particles were detected by FISH analysis in about 60-70% of myofiber nuclei in muscles of injected and contralateral limbs 7 days after application. The presence of human dystrophin cDNA and its products in all skeletal muscles and in different internal organs was proven by PCR and RT-PCR analysis. Patches of beta-galactosidase expression were abundant in injected muscle, and frequent in the contralateral and other skeletal muscles as well as in diaphragm, heart and lungs. High levels of dystrophin cDNA expression, and an efficient distant transfection effect with preferential intranuclei inclusion of MF-2 vehicle, are very encouraging for the development of a new constructive strategy in gene therapy trials of DMD.
authors.affiliation: Ott's Institute of Obstetrics and Gynecology, St Petersburg, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Baranov
authors.forename: P
authors.initials: P
authors.lastname: Glazkov
authors.forename: A
authors.initials: A
authors.lastname: Kiselev
authors.forename: O
authors.initials: O
authors.lastname: Ostapenko
authors.forename: V
authors.initials: V
authors.lastname: Mikhailov
authors.forename: T
authors.initials: T
authors.lastname: Ivaschenko
authors.forename: V
authors.initials: V
authors.lastname: Sabetsky
authors.forename: V
authors.initials: V
authors.lastname: Baranov
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000008:administration & dosage; D016189:Dystrophin / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D007273:Injections, Intramuscular; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D018101:Mice, Inbred mdx; D008863:Microspheres; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D014162:Transfection / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D016189:Dystrophin; D001616:beta-Galactosidase