title: Ploidy analysis and S-phase fraction determination by flow cytometry in anembryonic pregnancy and spontaneous abortions.
abstract: The nuclear DNA content of 20 anembryonic pregnancies was studied by flow cytometry from paraffin embedded tissue blocks. An abnormal amount of DNA content was found in 8 of the cases. This was a significantly higher percentage than encountered in spontaneous abortions studied by the same population (40 and 9%, respectively, p < 0.05). The S-phase fraction in anembryonic pregnancies was lower than in spontaneous abortions (22.4 +/- 12.7 and 35.4 +/- 6.8, respectively, p < 0.01). The results indicated that abnormal embryogenesis with grave chromosomal aberrations may play a major role in the etiology of anembryonic pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ozeren
authors.forename: V
authors.initials: V
authors.lastname: Aydemir
authors.forename: Y
authors.initials: Y
authors.lastname: Tekelioglu
authors.forename: K
authors.initials: K
authors.lastname: Topçuoglu
authors.forename: H
authors.initials: H
authors.lastname: Bozkaya
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics* / Q000473:pathology; D000328:Adult; D002869:Chromosome Aberrations / Q000235:genetics* / Q000473:pathology; D025063:Chromosome Disorders; D004247:DNA / Q000032:analysis / Q000235:genetics; D004622:Embryo, Mammalian; D005260:Female; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D011003:Ploidies; D011247:Pregnancy / Q000235:genetics*; D016196:S Phase / Q000502:physiology*
chemical_list: D004247:DNA