A search for a mutation in the tumour necrosis factor-alpha gene in narcolepsy.
The discovery of almost 100% association of narcolepsy with human leukocyte antigens (HLA) DR2 antigen prompted molecular biological research of this disorder. In the HLA class II gene cluster, the gene for tumour necrosis factor-alpha (TNF-alpha), which plays a role in the regulation of normal human sleep, is located. The present study searched for a mutation in the TNF-alpha gene by single-strand conformation polymorphism analysis (SSCP) in patients with narcolepsy. No mutation was detected in exons and introns of the TNF-alpha gene by SSCP and sequencing.
Kato
T
T
Department of Neuropsychiatry, Faculty of Medicine, University of Tokyo, Japan. tadafumitky@umin.ac.jp
Honda
M
M
Kuwata
S
S
Juji
T
T
Fukuda
M
M
Honda
Y
Y
Kato
N
N
D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D015802:HLA-DR2 Antigen / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009290:Narcolepsy / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D015802:HLA-DR2 Antigen; D014409:Tumor Necrosis Factor-alpha