title: Bacterial inactivation by using near- and supercritical carbon dioxide.
abstract: The three most common methods of sterilization in use today are ethylene oxide exposure, gamma-irradiation, and steam sterilization. Each of these methods has serious limitations for the sterilization of some materials used in medicine, especially thermally and hydrolytically sensitive polymers by themselves and in combination with proteins. In this work, we demonstrate a potential new method of sterilization by using supercritical fluid carbon dioxide. Using this method we achieve complete inactivation of a wide variety of bacterial organisms at moderate temperatures and in the absence of organic solvents or irradiation. Sterilization is a function of both the proximity to the fluid's critical point and the chemical nature of the fluid itself. When biodegradable polymers poly(lactic-co-glycolic) acid and polylactic acid were included in the sterilization process, there was no effect on the inactivation efficiency, yet no physical or chemical damage to these thermally and hydrolytically labile materials was observed.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Dillow
authors.forename: F
authors.initials: F
authors.lastname: Dehghani
authors.forename: J S
authors.initials: JS
authors.lastname: Hrkach
authors.forename: N R
authors.initials: NR
authors.lastname: Foster
authors.forename: R
authors.initials: R
authors.lastname: Langer
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000494:pharmacology*; D007700:Kinetics; D019344:Lactic Acid; D008863:Microspheres; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D017550:Spectroscopy, Fourier Transform Infrared; D013171:Spores, Bacterial; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*
chemical_list: D011108:Polymers; D002245:Carbon Dioxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid