Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters.
We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.
Kato
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A
Department of Agricultural Chemistry, Kinki University, 3327-204, Nakamachi, Nara 631-8505, Japan.
Tanabe
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H
Utsumi
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R
D000251:Adenosine Triphosphatases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008274:Magnesium; D026901:Membrane Transport Proteins; D011401:Promoter Regions, Genetic; D015398:Signal Transduction
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C079792:PhoQ protein, Bacteria; C061064:PhoP protein, Bacteria; D000251:Adenosine Triphosphatases; C490561:MgtA protein, E coli; C093585:MgtA protein, bacteria; D008274:Magnesium