title: Plasmid profiles of multiple drug resistant local strains of Staphylococcus aureus.
abstract: In an attempt to determine the molecular basis for the degree of resistance among strains of S. aureus to Beta-lactam antibiotics, 50 clinical isolates of coagulase-positive strains of S. aureus were obtained. The strains and a control strain, Oxford NCTC 6571, were screened for the presence of Beta-lactamase, R-plasmid, and sensitivity to six Beta-lactam antibiotics. The molecular weight of the isolated R-plasmid DNA was determined by agarose gel electrophoresis. Forty-five of the 50 strains of S. aureus exhibited single or multiple drug-resistance. Plasmid DNA isolated from 13 of the resistant strains of S. aureus had the same molecular weight of 21 kb or 13.63 MDa, while one of the 13 strains and 3 others had R-plasmids with a higher molecular weight.
authors.affiliation: Department of Pharmaceutical Microbiology and Clinical Pharmacy, University of Ibadan, Nigeria.
authors.forename: O E
authors.initials: OE
authors.lastname: Adeleke
authors.forename: H A
authors.initials: HA
authors.lastname: Odelola
mesh_terms: D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D017053:Infection Control; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D009549:Nigeria; D011815:R Factors / Q000235:genetics*; D012703:Serotyping; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases