Induction of apoptosis of monocyte-macrophage lineage cells by 5-S-GAD.
We found that 5-S-GAD, an insect-derived antibacterial peptide, inhibited murine osteoclast formation in vitro. We examined the specific time point of the inhibitory action of 5-S-GAD on osteoclast formation and found that it mainly suppressed differentiation of osteoclasts in the middle of the culture period. Using HL60 cells that are able to differentiate into multinucleated macrophage-like cells, we found that 5-S-GAD induced apoptosis of HL60 cells by producing H(2)O(2). Thus, the inhibition of osteoclast formation by 5-S-GAD could be, in part, due to apoptosis of the cells of an osteoclast lineage.
Hijikata
M
M
Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Matsumoto
H N
HN
Kobayashi
A
A
Nifuji
A
A
Noda
M
M
Natori
S
S
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D002117:Calcitriol / Q000494:pharmacology; D019070:Cell Lineage; D002478:Cells, Cultured; D018920:Coculture Techniques; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005452:Fluoresceins / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000187:drug effects* / Q000473:pathology; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000071681:Tartrate-Resistant Acid Phosphatase
D000890:Anti-Infective Agents; D005452:Fluoresceins; D007527:Isoenzymes; C100496:N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine; C029569:diacetyldichlorofluorescein; D004295:Dihydroxyphenylalanine; D006861:Hydrogen Peroxide; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002117:Calcitriol; D005978:Glutathione