Growth of human lung adenocarcinoma in nude mice is influenced by various types of dietary fat and vitamin E.
Studies have shown effects of dietary lipids on carcinogenesis and tumour progression. Different mechanisms for the inhibitory effect of n-3 fatty acids (FA) have been proposed. The inhibition of the growth of subcutaneously transplanted A427 lung adenocarcinoma cells in athymic nude mice may occur due to an increased level of lipid peroxidation products and is the object of this study. The nude mice were fed diets supplemented with corn oil (CO), olive oil (OO) or K85, a mixture of ethyl esters of n-3 FAs, mainly eicosapentaenoic acid (EPA, 20:5, n-3) and docosahexaenoic acid (DHA, 22:6, n-3). Tumours of the n-3 FA group showed reduced growth. Peroxidation products measured by the thiobarbituric acid reactive substances (TBARS) test showed higher levels in tumours from n-3 FA fed mice than in the other diet groups. The growth inhibitory effects and the elevated level of TBARS in the n-3 FA diet group were counteracted by vitamin E supplement in the diet. Cu/Zn-superoxide dismutase (SOD) activity in liver did not differ greatly among the diet groups. The Ki-67 labelling index (LI), indicating cell proliferation rate was significantly lower in the K85 diet group compared to the other diet groups.
Maehle
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L
Department of Toxicology, National Institute of Occupational Health, Oslo, Norway. lovise.mahle@klinmed.uio.no
Lystad
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Eilertsen
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Einarsdottír
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E
Høstmark
A T
AT
Haugen
A
A
D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D003314:Corn Oil / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018450:Disease Progression; D004357:Drug Synergism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000032:analysis; D014183:Transplantation, Heterologous; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; C092589:K-85 fish oil preparation; D019394:Ki-67 Antigen; D000069463:Olive Oil; D010938:Plant Oils; D014810:Vitamin E; D003314:Corn Oil; D013482:Superoxide Dismutase