title: The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule.
abstract: Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC-->M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8(+) T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.
authors.affiliation: Children's Research Institute, Children's Hospital, Inc., Department of Pediatrics, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio, USA.
authors.forename: M
authors.initials: M
authors.lastname: Furchner
authors.forename: A L
authors.initials: AL
authors.lastname: Erickson
authors.forename: T
authors.initials: T
authors.lastname: Allen
authors.forename: D I
authors.initials: DI
authors.lastname: Watkins
authors.forename: A
authors.initials: A
authors.lastname: Sette
authors.forename: P R
authors.initials: PR
authors.lastname: Johnson
authors.forename: C M
authors.initials: CM
authors.lastname: Walker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics* / Q000276:immunology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins