Cytarabine-induced destabilization and bending of a model Okazaki fragment.
The effects of cytarabine on the structural and thermodynamic properties of an Okazaki fragment were investigated using UV hyperchromicity and 2D 1H NMR. Cytarabine significantly decreased the stability of this model Okazaki fragment, decreasing the melting temperature from 46.8 degrees C to 42.4 degrees C at 1.33 x 10(-5) M. Cytarabine also markedly increased the bend angle of the Okazaki fragment duplex from 20 degrees to 42 degrees. Changes to the structures and stabilities of Okazaki fragments may cause the biological effects of cytarabine.
Gmeiner
W H
WH
Eppley Institute, University of Nebraska Medical Center, Omaha 68198-6805, USA.
Skradis
A
A
Pon
R T
RT
Liu
J
J
D000964:Antimetabolites, Antineoplastic / Q000737:chemistry*; D001483:Base Sequence; D003561:Cytarabine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004261:DNA Replication; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
D000964:Antimetabolites, Antineoplastic; C022262:Okazaki fragments; D003561:Cytarabine; D004247:DNA