[Study on optimal conditions for the determination of glucose by measurement of oxygen consumption rate].
We studied optimal conditions for the determination of oxygen consumption rate (OCR). The assay, with a working range up to 35 mmol/L, had an intra-assay coefficient of variation of 0.7%-3.52%, and the inter-assay coefficient of variation was 4.86%. In spiking experiments, the recovery range was 98.9%-101%. The assay was compared with CX3 (Beckman Co.). A linear regression analysis yielded a slope of 1.05, an inercept of 3.82 and a correlation coefficient of 0.999. The serum of turbidity above +2 had a negative interference on the assay (> 3.4%). The obvious interference of hemolysis, icterus and vitamin C to the assay were not observed.
Liu
F
F
PUMC Hospital, Beijing.
Li
X
X
Shen
Y
Y
D001786:Blood Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006801:Humans; D010101:Oxygen Consumption
D001786:Blood Glucose; D005949:Glucose Oxidase