Effect of an aluminum hydroxide-magnesium hydroxide combination drug on adhesion, IL-8 inducibility, and expression of HSP60 by Helicobacter pylori.
BACKGROUND
Co-magaldrox (Maalox) is used world-wide as an antacid and as a cytoprotective agent for gastritis and peptic ulcer diseases. We examined the effects of co-magaldrox on Helicobacter pylori.


METHODS
Adhesion of H. pylori to human gastric epithelial cells (MKN45) was evaluated by flow cytometry. Morphologic changes in H. pylori caused by co-magaldrox were determined by scanning electron microscopy. Induction of interleukin-8 (IL-8) from MKN45 cells was examined with enzyme-linked immunosorbent assay, and the intracellular and extracellular expression of heat-shock protein 60 (HSP60) was analyzed with sodium dodecyl sulphate-polyacrylamide gel electrophoresis and flow cytometry.


RESULTS
Adhesion of H. pylori to MKN 45 cells was significantly inhibited by 1.25%-5% comagaldrox. H. pylori aggregated with co-magaldrox according to an electron microscopic examination. IL-8 secretion from MKN45 cells after H. pylori infection was also inhibited by co-magaldrox. Extracellular expression of HSP60 on the surface of H. pylori was decreased after treatment with comagaldrox, whereas the intracellular synthesis of HSP60 was not. HSP60-induced IL-8 secretion was significantly inhibited by co-magaldrox in a dose-dependent manner.


CONCLUSIONS
These results show that co-magaldrox suppressed the expression of the following virulence factors: adhesion, IL-8 inducibility, and expression of extracellular HSP60. Therefore, co-magaldrox is a potent anti-H. pylori and cytoprotective drug.
Kamiya
S
S
Dept. of Microbiology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Yamaguchi
H
H
Osaki
T
T
Taguchi
H
H
Fukuda
M
M
Kawakami
H
H
Hirano
H
H
D000536:Aluminum Hydroxide / Q000494:pharmacology*; D000863:Antacids / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D018834:Chaperonin 60 / Q000096:biosynthesis; D004338:Drug Combinations; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000382:microbiology* / Q000502:physiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D015151:Immunoblotting; D016209:Interleukin-8 / Q000378:metabolism; D008276:Magnesium Hydroxide / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning
D000863:Antacids; D018834:Chaperonin 60; D004338:Drug Combinations; D016209:Interleukin-8; C013591:aluminum hydroxide, magnesium hydroxide, drug combination; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide