title: A new method with general diagnostic utility for the calculation of immunoglobulin G avidity.
abstract: The reference method for immunoglobulin G (IgG) avidity determination includes reagent-consuming serum titration. Aiming at better IgG avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. This method was tested with well-characterized serum panels for cytomegalovirus, Epstein-Barr virus, rubella virus, parvovirus B19, and Toxoplasma gondii. This approach for IgG avidity calculation is generally applicable and attains the diagnostic performance of the reference method while being less laborious and twice as cost-effective.
authors.affiliation: Haartman Institute, Department of Virology, University of Helsinki, Helsinki, Finland.
authors.forename: M H
authors.initials: MH
authors.lastname: Korhonen
authors.forename: J
authors.initials: J
authors.lastname: Brunstein
authors.forename: H
authors.initials: H
authors.lastname: Haario
authors.forename: A
authors.initials: A
authors.lastname: Katnikov
authors.forename: R
authors.initials: R
authors.lastname: Rescaldani
authors.forename: K
authors.initials: K
authors.lastname: Hedman
mesh_terms: D000913:Antibodies, Protozoan / Q000032:analysis / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D003362:Cost-Benefit Analysis; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology*; D007159:Immunologic Tests / Q000191:economics / Q000379:methods* / Q000592:standards; D016015:Logistic Models; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000276:immunology; D012015:Reference Standards; D012409:Rubella / Q000175:diagnosis / Q000276:immunology; D017186:Titrimetry; D014123:Toxoplasmosis / Q000175:diagnosis / Q000276:immunology; D014777:Virus Diseases / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D007074:Immunoglobulin G