title: Primary cultures of heart cells from the scallop Pecten maximus (mollusca-bivalvia).
abstract: Primary cultures of Pecten maximus heart cells, isolated by an enzymatic procedure, were routinely obtained with a high level of reproducibility in a simple medium based on sterile seawater. Cells attached to the plastic substratum without the need to add a special factor. The number of adhering cells gradually increased with the time of culture. Two types of adhering cells were observed: epitheliallike cells and fibroblastlike cells, which were more numerous. The latter cells were identified as myocytes by electron microscopy and immunofluorescent staining. Results obtained by autoradiography, after incorporation of [14C]leucine, [3H]thymidine, and [14C]acetate, confirmed functional activity of the cells. These cultures were maintained viable in vitro during at least 1 mo.
authors.affiliation: Unité de Culture cellulaire, Université de Bretagne Occidentale, Brest, France.
authors.forename: F
authors.initials: F
authors.lastname: Le Marrec-Croq
authors.forename: D
authors.initials: D
authors.lastname: Glaise
authors.forename: C
authors.initials: C
authors.lastname: Guguen-Guillouzo
authors.forename: C
authors.initials: C
authors.lastname: Chesne
authors.forename: A
authors.initials: A
authors.lastname: Guillouzo
authors.forename: V
authors.initials: V
authors.lastname: Boulo
authors.forename: G
authors.initials: G
authors.lastname: Dorange
mesh_terms: D000818:Animals; D001345:Autoradiography; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008974:Mollusca / Q000166:cytology*; D009206:Myocardium / Q000166:cytology; D012623:Seawater; D013997:Time Factors
chemical_list: D003470:Culture Media; D004247:DNA