Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein.
Human protein 1/Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein 1, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.
Yamaguchi
T
T
Department of Clinical Pathology, Jichi Medical School, Tochigi-Ken, Japan.
Yamada
T
T
Okutani
R
R
Shijubo
N
N
Singh
G
G
Itoh
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004847:Epithelial Cells / Q000737:chemistry / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D008168:Lung / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D014598:Uteroglobin
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011506:Proteins; D011994:Recombinant Proteins; C556389:SCGB1A1 protein, human; C556390:Scgb1a1 protein, mouse; D014598:Uteroglobin