title: Enantioselective recognition mechanism of secondary alcohol by surfactant-coated lipases in nonaqueous media.
abstract: The enantioselective recognition mechanism of secondary alcohol by lipases originated from Candida rugosa and Pseudomonas cepacia was elucidated on the basis of the kinetic study of the esterification of alcohol with lauric acid in isooctane. To obtain inherent kinetic parameters, we utilized a surfactant-coated lipase whose conformation is considered to be an "open" form in a homogeneous organic solvent. Based on the experimental results, the enantioselectivity of lipases was found to be derived from the difference in the V(max) values between the two enantiomers. The same result was observed when lipases of different origin and substrates with different molecular structures were applied. &copy 1999 John Wiley & Sons, Inc.
authors.affiliation: Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, 6-10-1, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kamiya
authors.forename: H
authors.initials: H
authors.lastname: Kasagi
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Kusunoki
authors.forename: M
authors.initials: M
authors.lastname: Goto
mesh_terms: D000438:Alcohols / Q000032:analysis*; D001592:Benzyl Alcohols; D016956:Burkholderia cepacia / Q000201:enzymology; D002175:Candida / Q000201:enzymology; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008968:Molecular Conformation; D000442:Octanols; D012997:Solvents; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D000438:Alcohols; D001592:Benzyl Alcohols; D000442:Octanols; D012997:Solvents; D013501:Surface-Active Agents; C002017:methylphenyl carbinol; D008049:Lipase