title: Urinary neopterin and kynurenine in patients submitted to surgical stress with different inhalational anesthetics (halothane or isoflurane).
abstract: Measurements were taken of urinary levels of neopterin (NPT) and kynurenine (KYN), using an HPLC method for their simultaneous analysis in patients submitted to anesthetical surgical stress with two different inhalational anesthetics (halothane and isoflurane). We studied twenty-one women affected by uterine fibromyomatosis and submitted to total hysterectomy (mean age of 42.7+/-5.4 years). They showed the same pre-operative evaluation (ASA-1), and underwent the same i.v. anesthetic treatment. Our patients were randomized in two groups: Group A: 11 patients had halothane as an inhalational anesthetic drug for the maintenance of the anesthetic induction (mean time= 1 h). Group B: 10 patients had isoflurane. A significant decrease in urinary NPT and KYN, parallel to serum-NPT, was found 4 h after anesthetic induction. Raised NPT levels appeared 24 h after A.I. with significant increased levels after 7 days. A strong correlation between urinary and serum NPT levels was seen (Rs= 0.74; p < 0.001). Significantly low KYN levels were observed both 4 h and 24 h after A.I.. In addition to the delayed increase of the excretory KYN levels, significantly raised KYN levels in Group B (isoflurane) 48 h after A.I. (10.59+/-14.31 vs 5.99+/-7.17 micromol/mol creat.; p < 0.01) were shown, whereas in Group A (halothane) we observed a progressive increase as compared to the pre-surgery values starting from 72 h after surgery. Our data seem to show that: (a) it is possible to have a biochemical and non invasive monitoring of the anesthetical-surgical stress on MM "priming" activity; (b) the activation of the phagocyte compartment is one of the earlier immunological events after surgery (NPT), but the efficiency of this "priming" appears to be delayed (KYN); (c) isoflurane appears to induce an earlier recovery in MM activation.
authors.affiliation: Servizio di Patologia Clinica, Istituto Nazionale per lo Studio e la Cura dei Tumori Fondazione Sen. G. Pascale, Napoli, Italy. gsantelli@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Marfella
authors.forename: A
authors.initials: A
authors.lastname: Bilancio
authors.forename: C
authors.initials: C
authors.lastname: Polese
authors.forename: F
authors.initials: F
authors.lastname: Iodice
authors.forename: C
authors.initials: C
authors.lastname: Edmondo
authors.forename: D
authors.initials: D
authors.lastname: Cerasuolo
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: V
authors.initials: V
authors.lastname: Zannoni
authors.forename: G
authors.initials: G
authors.lastname: Beneduce
mesh_terms: D000328:Adult; D000768:Anesthesia, General / Q000009:adverse effects*; D018685:Anesthetics, Inhalation / Q000009:adverse effects* / Q000627:therapeutic use; D005260:Female; D006221:Halothane / Q000009:adverse effects / Q000627:therapeutic use; D006801:Humans; D007044:Hysterectomy / Q000009:adverse effects; D007166:Immunosuppressive Agents / Q000009:adverse effects / Q000627:therapeutic use; D007530:Isoflurane / Q000009:adverse effects* / Q000627:therapeutic use; D007737:Kynurenine / Q000652:urine*; D007889:Leiomyoma / Q000276:immunology / Q000601:surgery; D008875:Middle Aged; D019798:Neopterin / Q000652:urine*; D013312:Stress, Physiological / Q000209:etiology / Q000276:immunology / Q000652:urine*; D014594:Uterine Neoplasms / Q000276:immunology / Q000601:surgery
chemical_list: D018685:Anesthetics, Inhalation; D007166:Immunosuppressive Agents; D007737:Kynurenine; D019798:Neopterin; D007530:Isoflurane; D006221:Halothane