title: Altered development of dopaminergic cells in the retina of weaver mice.
abstract: Postnatal degeneration of dopaminergic (DA) cells is known to occur in mesencephalic nuclei of mutant weaver mice, whereas retinal DA content is reported to be unchanged in the adult animal. To determine whether morphological changes occur in the weaver retinal DA system, we compared weaver and control developing and adult retinas after tyrosine hydroxylase (TH) immunohistochemistry. The density and distribution of DA cells were analyzed using Dirichlet tessellation. Not only was no DA cell loss found in adult weaver retinas, but we even observed an increase in DA cells in weaver compared to control retinas between postnatal days 14 and 30. Furthermore, some unusual features were found during the latter period: atypical cells (representing a maximum of 12% of the whole DA cell population) were observed, and these differed from typical DA cells in terms of both location (slightly more external within the inner nuclear layer) and appearance (flat somata, round and clear nuclei, thick dendritic trunks emerging laterally and giving rise to horizontal processes). Some of the atypical cells were intermingled in a delicate network lying in a more outer focal plane than the main DA plexus. The expression of GIRK2, a G protein-related inward rectifying K(+) channel responsible for the weaver syndrome, was investigated. Although no GIRK2 labeling was demonstrated in DA cells, its possible involvement in the transient disturbances observed in the weaver DA retinal system is discussed.
authors.affiliation: Laboratoire de Neurocytologie Oculaire, INSERM U86, 75270 Paris Cedex 06, France. savy@ccr.jussieu.fr
authors.forename: C
authors.initials: C
authors.lastname: Savy
authors.forename: E
authors.initials: E
authors.lastname: Martin-Martinelli
authors.forename: A
authors.initials: A
authors.lastname: Simon
authors.forename: C
authors.initials: C
authors.lastname: Duyckaerts
authors.forename: C
authors.initials: C
authors.lastname: Verney
authors.forename: C
authors.initials: C
authors.lastname: Adelbrecht
authors.forename: R
authors.initials: R
authors.lastname: Raisman-Vozari
authors.forename: J
authors.initials: J
authors.lastname: Nguyen-Legros
mesh_terms: D000818:Animals; D004298:Dopamine / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051379:Mice; D008818:Mice, Neurologic Mutants; D012016:Reference Values; D012160:Retina / Q000166:cytology / Q000254:growth & development*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine