title: Clostridium difficile cell attachment is modified by environmental factors.
abstract: Adherence of Clostridium difficile to Vero cells under anaerobic conditions was increased by a high sodium concentration, calcium-rich medium, an acidic pH, and iron starvation. The level of adhesion of nontoxigenic strains was comparable to that of toxigenic strains. Depending on the bacterial culture conditions, Vero cells could bind to one, two, or three bacterial surface proteins with molecular masses of 70, 50, and 40 kDa.
authors.affiliation: Département de Microbiologie, Faculté de Pharmacie, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France.
authors.forename: A J
authors.initials: AJ
authors.lastname: Waligora
authors.forename: M C
authors.initials: MC
authors.lastname: Barc
authors.forename: P
authors.initials: P
authors.lastname: Bourlioux
authors.forename: A
authors.initials: A
authors.lastname: Collignon
authors.forename: T
authors.initials: T
authors.lastname: Karjalainen
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000328:Adult; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002648:Child; D002522:Chlorocebus aethiops; D016360:Clostridioides difficile / Q000502:physiology*; D003470:Culture Media; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D014709:Vero Cells
chemical_list: D018829:Adhesins, Bacterial; D001427:Bacterial Toxins; D003470:Culture Media; D008274:Magnesium; D002118:Calcium