In vitro study on cytotoxic effect of some chemicals on serum McCoy and serum-free McCoy-Plovdiv cell culture systems.
The cytotoxicity of test agents on serum-free McCoy cultures has not been studied at all. The cytotoxic effect of EDTA, methanol, DMSO, and cycloheximide on serum-free McCoy-Plovdiv cell culture (SF) was detected visually on inverted microscope and quantitatively by tests for viability (NR) and total protein (KBP). The IC50 values for the tested chemicals were calculated. SF showed the lowest IC50 values for cycloheximide, DMSO and EDTA and the highest for methanol according to both tests. EDTA, methanol, DMSO and cycloheximide had dose-effect relationship in the cell test systems after treatment. The data indicate that McCoy-Plovdiv cell line is a suitable serum-free cell system for in vitro cytotoxic studies.
Docheva
D
D
Cell Biology Laboratory, Faculty of Biology, University of Plovdiv, Bulgaria.
Draganov
M
M
Murdjeva
M
M
D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002614:Chelating Agents / Q000633:toxicity; D016895:Culture Media, Serum-Free; D003513:Cycloheximide / Q000633:toxicity; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004492:Edetic Acid / Q000633:toxicity; D006801:Humans; D000432:Methanol / Q000633:toxicity; D012997:Solvents / Q000633:toxicity
D002614:Chelating Agents; D016895:Culture Media, Serum-Free; D012997:Solvents; D003513:Cycloheximide; D004492:Edetic Acid; D000432:Methanol; D004121:Dimethyl Sulfoxide