Renaturation of rabbit liver aminoacyl-tRNA synthetases by 80S ribosomes.
Protein biosynthesis machinery is thought to be mostly compartmentalised within the mammalian cell, involving direct interactions between different components of the translation apparatus. The present research concerns the functional meaning of the interaction between the rabbit liver aminoacyl-tRNA synthetases and 80S ribosomes. We have shown that rabbit liver 80S ribosomes are able to enhance the activity of leucyl-tRNA synthetase, which is a component of high-molecular weight aminoacyl-tRNA synthetase complex, and phenylalanyl-tRNA synthetase not associated within this complex. The ribosomes increase the initial rate of both the total reaction of tRNA aminoacylation and the first step of this reaction, the formation of leucyladenylate. Moreover, a positive cooperativity of the tRNA interaction with two binding sites of leucyl-tRNA synthetase is also increased in the presence of highly purified 80S ribosomes. The effect of 80S ribosomes on partly denatured leucyl-tRNA synthetase and phenylalanyl-tRNA synthetase and the protection by 80S ribosomes of both enzymes against inactivation indicate a refolding and stabilising capacity of the ribosomes. It is concluded that the interaction of aminoacyl-tRNA synthetases and 80S ribosomes is important for the maintenance of an active conformation of the enzymes.
Turkovskaya
H V
HV
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Belyanskaya
L L
LL
Kovalenko
M I
MI
El'skaya
A V
AV
D000604:Amino Acyl-tRNA Synthetases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D004795:Enzyme Stability; D006358:Hot Temperature; D066298:In Vitro Techniques; D007700:Kinetics; D007935:Leucine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D010652:Phenylalanine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D011489:Protein Denaturation; D011817:Rabbits; D012270:Ribosomes / Q000737:chemistry / Q000201:enzymology*
D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase; D007935:Leucine-tRNA Ligase