title: Overcoming false negatives due to the genomic context in polymerase chain reaction amplification.
abstract: In some instances defined genomic regions are so poorly amplified that they seem to be unamplifiable. A protocol was developed which allows good PCR amplifications by the use of restriction digestion combined with the elution of a pool of restriction fragments of defined size range from agarose gel after electrophoresis. We describe the application of the method in the PCR amplification of a region of the white locus of Drosophila melanogaster that otherwise may be considered as a negative result.
authors.affiliation: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Baldrich
authors.forename: N
authors.initials: N
authors.lastname: Xamena
authors.forename: O
authors.initials: O
authors.lastname: Cabré
mesh_terms: D000818:Animals; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005188:False Negative Reactions; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004262:DNA Restriction Enzymes