Transfection of the human malaria parasite Plasmodium falciparum.
In the past few years, methods have been developed which allow the introduction of exogenous DNA into the human malaria parasite Plasmodium falciparum. This important technical advance known as parasite transfection, provides powerful new tools to study the function of Plasmodium proteins and their roles in biology and disease. Already it has allowed the analysis of promoter function and has been successfully applied to establish the role of particular molecules and/or mutations in the biology of this parasite. This review summarises the current state of the technology and how it has been applied to dissect the function of the P. falciparum genome.
Waterkeyn
J G
JG
Division of Infection and Immunity, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia. waterkeyn@wehi.edu.au
Crabb
B S
BS
Cowman
A F
AF
D000818:Animals; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods*