title: Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea.
abstract: SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-D-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.
authors.affiliation: Department of Nuclear Medicine, Royal Marsden NHS Trust and Institute of Cancer Research, Sutton, Surrey, UK. t.smith@icr.ac.uk
authors.forename: T A
authors.initials: TA
authors.lastname: Smith
authors.forename: J C
authors.initials: JC
authors.lastname: Titley
mesh_terms: D019325:3-O-Methylglucose / Q000493:pharmacokinetics*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D005434:Flow Cytometry; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D019325:3-O-Methylglucose; D004247:DNA; D003847:Deoxyglucose; D006918:Hydroxyurea