title: A significant relationship between glutathione-peroxidase (GSH-PO) localization and biological action of testosterone in rat ventral prostate.
abstract: In order to confirm the relationship between glutathione-peroxidase (GSH-PO) localization and biological testosterone action in the rat ventral prostate, immunocytochemical localization of GSH-PO in glandular epithelial cells of the rat ventral prostate was investigated. In the untreated group, GSH-PO was predominantly demonstrated in glandular epithelial cells of the ventral prostate. Intracellular localization of GSH-PO in the glandular epithelial cells was mainly observed in cytoplasmic matrix near the rough endoplasmic reticulum and was occasionally noted as a small granular structure (GSH-PO-positive granule) at the supranuclear region. In a castrated animal, the intensity of GSH-PO staining in the glandular epithelial cells was remarkably decreased. By testosterone administration to the castrated animal, GSH-PO was clearly detected in the glandular epithelial cells. Intracellular localization of GSH-PO was mainly observed in cytoplasmic matrix and the number of GSH-PO-positive granules increased remarkably. These findings suggest that immunostainable GSH-PO in the glandular epithelial cells of the rat ventral prostate is testosterone-dependent, and that its staining pattern is a useful marker for biological testosterone action.
authors.affiliation: Safety Research Department, Teikoku Hormone Mfg. Co., Ltd., Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Murakoshi
authors.forename: R
authors.initials: R
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Tagawa
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D009919:Orchiectomy; D011467:Prostate / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone; D005979:Glutathione Peroxidase