title: Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species.
abstract: The complete nucleotide sequence of RNA-2 from a Japanese isolate IP of broad bean wilt virus (BBWV) was determined. The sequence encodes a single large polyprotein, which contains a putative movement protein and two coat proteins (CPs). The 3'-terminal sequences of RNA-2 were also determined for three other Japanese isolates and two ATCC isolates (PV132 and PV176) of BBWV. The CPs of the four Japanese isolates share 86.8-98.0% amino acid sequences homology with one another and 88.3-96.5% with those reported for the isolate PV131 (BBWV-2). However, they have only 57.9-66.2% homology with those of PV132 and PV176 (BBWV-1).
authors.affiliation: National Agriculture Research Center, Tsukuba, Japan.
authors.forename: Y O
authors.initials: YO
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: S
authors.initials: S
authors.lastname: Kashiwazaki
authors.forename: T
authors.initials: T
authors.lastname: Naito
authors.forename: Y
authors.initials: Y
authors.lastname: Mikoshiba
authors.forename: A
authors.initials: A
authors.lastname: Shiota
authors.forename: M
authors.initials: M
authors.lastname: Kameya-Iwaki
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry; D019181:Fabavirus / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry*; D017385:Sequence Homology
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral