Evaluation of the effects of Gd complexes used as magnetic resonance imaging contrast agents, on superoxide dismutase: comparison of two methods.
Investigations are currently being made into the safety of gadolinium complex contrast agents used in Magnetic Resonance Imaging. Their hyperosmolality or potential Gd3+ release is evoked as a cause of various anaphylactoid reactions to be observed in humans after intravenous injection. An estimation has already been made of their effects on the liberation of reactive oxygen intermediates by neutrophils. The purpose of this study was to find a suitable method to measure SOD activity in the presence of these hyperosmolar solutions, and to evaluate their action on this activity. Two techniques were compared to measure this activity. Results and statistical analysis showed that pyrogallol autoxidation was greatly affected by solution osmolalities, whereas ferricytochrome C reduction was not. Gadopentetate dimeglumine and gadoterate meglumine seemed to activate Cu, Zn SOD in vitro, but did not exhibit any SOD-like activity. Gadodiamide did not interfere with this system of detoxication.
Behra-Miellet
J
J
Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2, France.
Gressier
B
B
Dine
T
T
Brunet
C
C
Luyckx
M
M
Ballester
L
L
Cazin
M
M
Cazin
J C
JC
D003287:Contrast Media / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005682:Gadolinium / Q000633:toxicity; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D013482:Superoxide Dismutase / Q000032:analysis* / Q000378:metabolism
D003287:Contrast Media; D005682:Gadolinium; D013482:Superoxide Dismutase