[Effect of calcium and angiotensin II receptor on angiotensin II-stimulated protein synthesis in neonatal myocardial cells].
In this study, the role of angiotensin II (Ang II) receptor, Ca2+ in the Ang II-stimulated neoeatal myocytes protein synthesis were investigated and the effect of Ang II on expression of c-fos gene was observed. The results showed that the stimulatory effect of Ang II on protein synthesis was blocked by the Ang II antagonist-[Sar1 Ile8] Ang II. When verapamil, EG-TA, dantrolene, and Fura-2/AM, were added to the medium, the 3H-leucine incorporations stimulated by Ang II were reduced by 17%, 19%, 22%, and 27%. Using calcium ionophore-A23187 did not stimulate protein synthesis, but enhanced Ang II-induced 3H-leucine incorporation. In addition, Ang II could induce expression of c-fos gene. These results indicated that the hypertrophic effect of Ang II on the myocytes was mediated by Ang II receptor and depended on increase in [Ca2+]i and may be associated with enhanced expression of c-fos.
Zhou
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X
Cardiovascular Institute, CAMS, Beijing.
Chen
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L
D000804:Angiotensin II / Q000494:pharmacology*; D057911:Angiotensin Receptor Antagonists; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D015870:Gene Expression; D016762:Genes, fos; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
D057911:Angiotensin Receptor Antagonists; D000804:Angiotensin II; D002118:Calcium