title: Detection of mycobacterial infections using the Dieterle stain.
abstract: Retrospective review comparing the modified Dieterle stain with standard acid-fast stains was performed on seven surgical pathology cases that contained culture-positive mycobacteria infections. Tissues examined comprised cervical and submandibular lymph nodes and soft tissues of the face and chest. Modified Dieterle staining was performed on paraffin-embedded tissue sections, and the results were compared with those of hematoxylineosin stains and auramine-rhodamines and carbolfuchsin acid-fast stains. The acid-fast stain showed organisms in three of seven cases on initial review and five of seven cases on retrospective review; the auramine-rhodamine stain retrospectively revealed organisms in five cases. In contrast, the Dieterle stain showed organisms in all seven sections on retrospective examination. Dieterle stains revealed either beaded bacilli or nocardia-like filamentous organisms, sometimes with abundant granular debris possibly representing degenerative organisms. In three cases in which bacteria were readily apparent with the Dieterle stain, only rare organisms could be identified with the acid-fast stain. The modified Dieterle stain was more sensitive than the acid-fast stain in the identification of mycobacteria in pediatric specimens, and its use is recommended in cases with necrotizing granulomas. However, its specificity is limited by morphologic similarities of the organisms to those of nocardiosis and cat scratch disease.
authors.affiliation: Department of Pathology, Slot 517, University of Arkansas for Health Sciences, Little Rock 72205, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Brady
authors.forename: G E
authors.initials: GE
authors.lastname: Schutze
authors.forename: R
authors.initials: R
authors.lastname: Seibert
authors.forename: H V
authors.initials: HV
authors.lastname: Horn
authors.forename: B
authors.initials: B
authors.lastname: Marks
authors.forename: D M
authors.initials: DM
authors.lastname: Parham
mesh_terms: D000293:Adolescent; D000328:Adult; D001576:Benzophenoneidum; D002372:Cat-Scratch Disease / Q000175:diagnosis / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D003937:Diagnosis, Differential; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D008297:Male; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000473:pathology*; D009617:Nocardia Infections / Q000175:diagnosis / Q000473:pathology; D012189:Retrospective Studies; D012235:Rhodamines; D012680:Sensitivity and Specificity
chemical_list: D004396:Coloring Agents; D012235:Rhodamines; D001576:Benzophenoneidum