Nitrated nonivamide displaying a drawback of proton's role in capsaicin-associated sensory and neuronal activities.
Nitrated nonivamide (NVANO)-induced triad hypotension, and biphasic bradycardia at 0.25-1.0 mg/kg (IV) was inhibited by capsazepine (1.0 mg/kg, IV), atropine (1.0 mg/kg, IV), and vagotomy in rats. NVANO also elicited a hypotensive spinal reflex at 5.0 mg/kg (IA). In the isolated rat vagus, NVANO (10.0-100.0 microM) revealed a sensory C-spike inhibition and membrane depolarization. NVANO (5.0 microM)-induced calcium influx in the isolated rat dorsal root ganglion cells (DRGs) was diminished by capsazepine (10.0 microM). In the isolated guinea pig atria, NVANO (1.0-50.0 microM)-induced positive inotropic and chronotropic activities were antagonized by capsazepine (1.0-10.0 microM) and human calcitonin gene-related peptide(8-37) (hCGRP(837); 0.1-1.0 microM).
Yang
Y C
YC
Department of Pharmacology, Graduate Institute of Medicine, Kaohsiung Medical College, Taiwan.
Ma
C R
CR
Yeh
J L
JL
Lo
Y C
YC
Wu
B N
BN
Lin
Y T
YT
Chen
I J
IJ
D000200:Action Potentials / Q000187:drug effects; D000577:Amides; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D017950:Ganglia, Sensory / Q000187:drug effects* / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006168:Guinea Pigs; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009566:Nitrates / Q000378:metabolism; D011522:Protons; D051381:Rats; D017208:Rats, Wistar; D014628:Vagotomy; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology; D014641:Vanillic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology
D000577:Amides; D004791:Enzyme Inhibitors; C121782:N-(4-hydroxy-5-nitro-3-methoxybenzyl)nonanamide; D009566:Nitrates; D011522:Protons; D014641:Vanillic Acid; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin; C040937:nonivamide; D002118:Calcium