Expanded expression of heme oxygenase-1 (HO-1) in the hypothalamic median eminence of aged as compared with young rats: an immunocytochemical study.
This study was performed to examine the differences in expression of heme oxygenase protein with age using immunocytochemistry. We compared the contents of HO-1 and HO-2 between young and aged rats using immunocytochemical methods. Stronger HO-1 expression was detected in the internal layer of the median eminence (ME) of aged than of young rats. Moreover, the cells expressing HO-1 were larger in the aged than the young animals. Electron microscopy indicated these cells with HO-1-like immunoreactivity (HO-1-LI) to be astrocytes. These findings suggested that the expression of HO-1 increased in the ME with age. The significance of this increased expression of HO-1 with age will be discussed briefly.
Iijima
N
N
Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Japan. niijima@basic.kpu-m.ac.jp
Tamada
Y
Y
Hayashi
S
S
Tanaka
M
M
Ishihara
A
A
Hasegawa
M
M
Ibata
Y
Y
D000375:Aging / Q000378:metabolism*; D000818:Animals; D005455:Fluorescent Antibody Technique; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis* / Q000276:immunology; D051547:Heme Oxygenase-1; D008473:Median Eminence / Q000737:chemistry / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley
D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1