[Effect of recombinant growth hormone on amino acids metabolism in blood and urine after major operation].
To determine the effects of recombinant human growth hormone (GH) on postoperative amino acids metabolism, we performed a placebo-controlled randomized double-blind trial in 18 patients after elective gastrectomy or colectomy. The same amount of calories and amino acids were administered. Research group was daily injected growth hormone 0.15 IU/(kg.day) and control group was given placebo during the first postoperative week. All urine were analyzed for nitrogen balance and excretion of 3-methylhistidine. On the seventh postoperative day while the parenteral nutrients were being infused, amino acid exchange across the forearm was measured. On the seventh postoperative day all patients' amino acids in plasma were slightly increased by comparing with preoperative day but was not statistical significance. Forearm arterial-venous difference (A-V) measured on POD + 7 was markedly different between groups. There was continued release of amino acids from the forearm in the control subjects, while there was marked amino acid uptake in the GH patients (total amino acid nitrogen exchange was -398 +/- 158 mumol/L vs +165 +/- 61 mumol/L, P < 0.01). Excretion of urinary 3-methylhistidine in control group loss more than in GH group (7.3 +/- 1.0 mumol/(kg.24 h) vs 4.4 +/- 0.3 mumol/(kg.24 h), P < 0.05). The results demonstrated that GH can increase the uptake of amino acid from forearm and decrease the excreation of 3-methylhistidine in urine after operation, but did not disturb the balance of amino acids in plasma.
He
G
G
PUMC Hospital, Beijing.
Jiang
Z
Z
Yang
N
N
Shu
H
H
Wilmore
D W
DW
D000597:Amino Acids, Branched-Chain / Q000097:blood; D000601:Amino Acids, Essential / Q000097:blood*; D003082:Colectomy; D004311:Double-Blind Method; D005743:Gastrectomy; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D008762:Methylhistidines / Q000652:urine*; D010649:Phenylalanine / Q000097:blood; D011182:Postoperative Care; D011446:Prospective Studies
D000597:Amino Acids, Branched-Chain; D000601:Amino Acids, Essential; D008762:Methylhistidines; D010649:Phenylalanine; D013006:Growth Hormone