Sampling considerations for herd-level measurement of faecal Escherichia coli antimicrobial resistance in finisher pigs.
The objective of this study was to determine the most efficient means of sampling faeces of finisher pigs for accurate and precise farm-level estimates of antimicrobial resistance among faecal Escherichia coli. Resistance to tetracycline and gentamicin of 8250 isolates of E. coli from 55 finisher pigs on one farm was measured with a hydrophobic grid membrane filter method. The between-pig, within-pen component of variance in resistance was large (97.5%), while between-pen, within-room and between-room components were small (2.5% and 0%, respectively). Using these resistance data, the abilities of two sampling strategies to estimate prevalence were modelled with a Monte Carlo 'bootstrap' procedure. Compositing faecal samples from several pigs before testing produced unbiased and precise estimates of prevalence and is simpler technically than individual animal testing.
Dunlop
R H
RH
Department of Population Medicine, University of Guelph, Ontario, Canada.
McEwen
S A
SA
Meek
A H
AH
Friendship
R M
RM
Black
W D
WD
Clarke
R C
RC
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D005260:Female; D005839:Gentamicins / Q000494:pharmacology*; D008297:Male; D008826:Microbial Sensitivity Tests; D011897:Random Allocation; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D013752:Tetracycline / Q000494:pharmacology*; D013753:Tetracycline Resistance
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D013752:Tetracycline