Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7.
The influence of growth temperature on the ability to ferment D-sorbitol was investigated in Escherichia coli O157:H7. It was found that O157:H7 strains have a temperature-sensitive sorbitol phenotype. D-Sorbitol transport and sorbitol-6-phosphate dehydrogenase activities were expressed in sorbitol-fermenting cells grown at 30 degrees C but only at a low level at 40 degrees C. Sorbitol-positive variants able to transport D-sorbitol were easily selected at 30 degrees C from culture of Sor(-) E. coli O157:H7 strains.
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Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur, 75724 Paris Cedex 15, France. obouvet@pasteur.fr
Pernoud
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Grimont
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D000693:Anaerobiosis; D019453:Escherichia coli O157 / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D008353:Mannitol / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases; D013696:Temperature
D008353:Mannitol; D013012:Sorbitol; D013401:Sugar Alcohol Dehydrogenases; C022645:sorbitol-6-phosphate dehydrogenase