Cell adhesion molecules in subretinal fluid: soluble forms of VCAM-1 (vascular cell adhesion molecule-1) and L-selectin.
OBJECTIVE
In this study we investigated the presence of soluble VCAM-1 and soluble L-selectin-1 in subretinal fluids (SRF) of patients suffering from rhegmatogenous retinal detachment .


METHODS
Subretinal fluids were collected from drainage sclerotomies during surgery from 27 patients with rhegmatogenous retinal detachment complicated by proliferative vitreoretinopathy (PVR) or uncomplicated retinal detachment. Levels of sVCAM-1 and sL-selectin-1 were quantified with ELISA.


RESULTS
The mean +/- SEM values of sVCAM-1 and sL-selectin-1 were 222.2 +/- 81 ng/ml and 171.7 +/- 42.1 ng/ml, respectively. The concentrations of sVCAM-1 in patients with Grade C PVR (498.2 +/- 1703 ng/ml) were significantly different from those with Grade B PVR (45.6 +/- 16.5 ng/ml) and uncomplicated retinal detachments (19.4 +/- 12.3 ng/ml). SVCAM-1 concentration in detachments which had been present for more than 8 weeks was 738.8 +/- 431 ng/ml, significantly higher than the levels in detachments of shorter duration (132.4 +/- 47.7 ng/ml). sL-selectin-1 level in Grade C PVR (291.6 +/- 92.8 ng/ml) was higher than in uncomplicated retinal detachments (72.8 +/- 13.5 ng/ml). Significantly elevated levels of sL-selectin-1 were observed in detachments lasting more than 8 weeks (605 +/- 151.1 ng/ml) compared to those of shorter duration (96.3 +/- 13.1 ng/ml).


CONCLUSIONS
The present study supports growing evidence that these cell adhesion molecules are involved in the inflammatory process during the development and progression of PVR.
Toker
E
E
Marmara University Faculty of Medicine, Department of Ophthalmology.
Kazokoğlu
H
H
Sahin
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers; D001826:Body Fluids / Q000378:metabolism*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D019041:L-Selectin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D013508:Ophthalmologic Surgical Procedures; D011379:Prognosis; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D012163:Retinal Detachment / Q000150:complications / Q000378:metabolism / Q000601:surgery; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000378:metabolism / Q000601:surgery
D015415:Biomarkers; D019010:Vascular Cell Adhesion Molecule-1; D019041:L-Selectin