title: Analysis of fusion junctions of circularized chromosomes in Streptomyces griseus.
abstract: A filamentous soil bacterium, Streptomyces griseus 2247, carries a 7. 8-Mb linear chromosome. We previously showed by macrorestriction analysis that mutagenic treatments easily caused deletions at both ends of its linear chromosome and changed the chromosome to a circular form. In this study, we confirmed chromosomal circularization by cloning and sequencing the junction fragments from two deletion mutants, 404-23 and N2. The junction sequences were compared with the corresponding right and left deletion end sequences in the parent strain, 2247. No homology and a 6-bp microhomology were found between the two deletion ends of the 404-23 and N2 mutants, respectively, which indicate that the chromosomal circularization was caused by illegitimate recombination without concomitant amplification. The circularized chromosomes were stably maintained in both mutants. Therefore, the chromosomal circularization might have occurred to prevent lethal deletions, which otherwise would progress into the indispensable central regions of the chromosome.
authors.affiliation: Department of Molecular Biotechnology, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Kameoka
authors.forename: A
authors.initials: A
authors.lastname: Lezhava
authors.forename: H
authors.initials: H
authors.lastname: Zenitani
authors.forename: K
authors.initials: K
authors.lastname: Hiratsu
authors.forename: M
authors.initials: M
authors.lastname: Kawamoto
authors.forename: K
authors.initials: K
authors.lastname: Goshi
authors.forename: K
authors.initials: K
authors.lastname: Inada
authors.forename: H
authors.initials: H
authors.lastname: Shinkawa
authors.forename: H
authors.initials: H
authors.lastname: Kinashi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping / Q000379:methods; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013305:Streptomyces griseus / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular