Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces.
Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)(12-18)] and oligothymidylic acids [d(pT)(12-24)] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min.
Sýkora
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Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Svec
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Fréchet
J M
JM
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D009841:Oligonucleotides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides