The subcellular localization of the carbohydrate epitope 3-fucosyl-N-acetyllactosamine is different in normal and reactive astrocytes.
The carbohydrate epitope 3-fucosyl-N-acetyllactosamine (CD15) is involved in cell-to-cell recognition processes in various tissues. In the present study the subcellular localization of CD15 was immunocytochemically studied in normal and pathological central nervous system fiber tracts of humans and rats. In normal human white matter of the brain, CD15 immunoreactivity was found on the cell surface of astrocytes and within the cytoplasm of oligodendrocytes. In freshly demyelinated lesions of two human diseases (central pontine myelinolysis and multiple sclerosis) strong cytoplasmic CD15 staining was observed in reactive astrocytes. In normal rats CD15 immunostaining was restricted to the surface of astrocytes. In crush-induced lesions of rat optic nerves, however, astrocytes showed a cytoplasmic localization of CD15, 4 and 6 days after injury. In conclusion, abnormal localization of CD15 in reactive astrocytes may be related to altered functional states of these cells during disease processes.
Gocht
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Abteilung für Neuroanatomie, Universität Hamburg, BRD.
D000818:Animals; D001253:Astrocytes / Q000737:chemistry*; D001923:Brain Chemistry; D003593:Cytoplasm / Q000737:chemistry; D000939:Epitopes / Q000032:analysis*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000032:analysis*; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology*; D017590:Myelinolysis, Central Pontine / Q000276:immunology*; D009836:Oligodendroglia / Q000737:chemistry; D009900:Optic Nerve / Q000473:pathology / Q000648:ultrastructure; D020221:Optic Nerve Injuries; D051381:Rats; D017208:Rats, Wistar
D000939:Epitopes; D005904:Glial Fibrillary Acidic Protein; D016256:Lewis X Antigen