Nitric oxide, heparin and procaine treatment in experimental ceruleine-induced acute pancreatitis in rats.
The aim of the study was to investigate the impact of L-arginine (nitric oxide donor), L-NNA (NO synthase inhibitor), heparin and procaine on the pancreas' microcirculation, serum interleukin 6 (IL-6) level, and microscopic alterations of the pancreatic gland in acute pancreatitis (AP) in rats. AP was induced by 4 i.p. injections of cerulein (15 micrograms/kg/h). Microcirculatory values of the pancreas were measured by means of laser Doppler flowmetry 5 h after the first cerulein injection. Remarkable morphologic changes in the pancreas, including parenchymal necrosis, an elevation of serum IL-6 activity, and significant drop of pancreatic capillary perfusion was observed in rats with NO synthase inhibition. L-arginine improved the pancreatic microcirculation but worsened the microscopic alterations within the pancreas. Heparin had a beneficial effect on the microcirculatory values, serum IL-6 activity, and morphologic changes. Procaine had no effect on the course of AP. Authors conclude that heparin, improving the pancreatic capillary blood perfusion, may be considered as a promising therapeutic agent in acute pancreatitis.
Dobosz
M
M
2nd Department of General, Gastroenterological and Endocrine Surgery, University Medical School, Gdańsk, Poland.
Wajda
Z
Z
Hać
S
S
Myśliwska
J
J
Bryl
E
E
Mionskowska
L
L
Roszkiewicz
A
A
Myśliwski
A
A
D000208:Acute Disease; D000818:Animals; D001120:Arginine / Q000627:therapeutic use; D002108:Ceruletide; D006493:Heparin / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009569:Nitric Oxide / Q000627:therapeutic use*; D019335:Nitroarginine / Q000627:therapeutic use; D010179:Pancreas / Q000098:blood supply; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D011343:Procaine / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D015850:Interleukin-6; D019335:Nitroarginine; D009569:Nitric Oxide; D011343:Procaine; D002108:Ceruletide; D006493:Heparin; D001120:Arginine