Two-dimensional protein maps of xenopus eggs and embryos at different developmental stages.
Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtained in four different ways: (1) immuno-blotting; (2) amino terminal sequence after blotting on to PVDF membranes; (3) comigration; and (4) assignment in comparison with proteins separated by 2D techniques on reference maps such as human liver, red blood cells, plasma and cerebrospinal fluid reported in the Swiss 2D-PAGE Data Base. The maps presented in this report are a step toward the study of the protein expression in Xenopus eggs and embryos and may be a powerful working tool since Xenopus embryos are popular models for the study of development.
Tedeschi
G
G
Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, 10 via Celoria, Milano, I-20133, Italy.
Bernardini
G
G
Mortarino
M
M
Negri
A
A
Ceciliani
F
F
Ronchi
S
S
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010063:Ovum / Q000378:metabolism; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D017421:Sequence Analysis / Q000379:methods; D014981:Xenopus / Q000196:embryology* / Q000378:metabolism
D011506:Proteins