title: Short report: evaluation of the potency and stability of a candidate vaccine against American cutaneous leishmaniasis.
abstract: Availability of a safe, immunogenic, and affordable vaccine would represent the best strategy for control of cutaneous leishmaniasis (CL). Stability in field conditions is a essential property for any candidate vaccine. The stability and immunogenicity of three different preparations (thimerosal-preserved, autoclaved, and lyophilized) of a killed Leishmania amazonensis vaccine were assessed using fresh products and after 12 months of storage at 4 degrees C. Autoclaving was associated with a time-dependent decrease in the immunogenicity of the vaccine, as measured by the leishmanin skin test and production of interferon-gamma. These findings are of importance in the decision of which preparation of candidate killed CL vaccines should move to phase III trials.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas/Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: W
authors.initials: W
authors.lastname: Mayrink
authors.forename: J
authors.initials: J
authors.lastname: Pinto
authors.forename: C
authors.initials: C
authors.lastname: Da Costa
authors.forename: V
authors.initials: V
authors.lastname: Toledo
authors.forename: T
authors.initials: T
authors.lastname: Guimarães
authors.forename: O
authors.initials: O
authors.lastname: Genaro
authors.forename: L
authors.initials: L
authors.lastname: Vilela
mesh_terms: D000328:Adult; D001938:Brazil; D004311:Double-Blind Method; D004355:Drug Stability; D004356:Drug Storage; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D008297:Male; D016052:Protozoan Vaccines; D015164:Vaccines, Inactivated
chemical_list: D016052:Protozoan Vaccines; D015164:Vaccines, Inactivated; D007371:Interferon-gamma