Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea.
SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-D-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.
Smith
T A
TA
Department of Nuclear Medicine, Royal Marsden NHS Trust and Institute of Cancer Research, Sutton, Surrey, UK. t.smith@icr.ac.uk
Titley
J C
JC
D019325:3-O-Methylglucose / Q000493:pharmacokinetics*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D005434:Flow Cytometry; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D019325:3-O-Methylglucose; D004247:DNA; D003847:Deoxyglucose; D006918:Hydroxyurea