title: Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes.
abstract: Chromosome-specific paint probes provide a powerful tool with wide applications in cytogenetic analysis. Here, we present a new approach using UV-laser microbeam microdissection in combination with laser-pressure catapulting, which allows the fast isolation of single chromosomes for the generation of chromosome-specific paint probes. To demonstrate the feasibility of this approach, single chromosomes were collected and amplified with degenerate oligonucleotide-primed PCR, hapten-labeled and hybridized onto normal metaphase spreads. Fluorescence in situ hybridization signals revealed specific painting of the respective chromosomes.
authors.affiliation: Ludwig Maximilians Universität, Munich, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Schermelleh
authors.forename: S
authors.initials: S
authors.lastname: Thalhammer
authors.forename: W
authors.initials: W
authors.lastname: Heckl
authors.forename: H
authors.initials: H
authors.lastname: Pösl
authors.forename: T
authors.initials: T
authors.lastname: Cremer
authors.forename: K
authors.initials: K
authors.lastname: Schütze
authors.forename: M
authors.initials: M
authors.lastname: Cremer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020223:Chromosome Painting / Q000379:methods*; D006224:Cricetinae; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence; D007834:Lasers; D016133:Polymerase Chain Reaction; D014466:Ultraviolet Rays
chemical_list: D015342:DNA Probes