[Smooth muscle myosin of SM1 and SM2 isoforms expressing human neuroblastoma cell line of MP-N-MS].
Human neuroblastoma (NB) cell lines have at least three morphological appearance of neuroblastic (N-type), substrate-adhessive (S-type) and intermediate(I) cells. Our previous study revealed S-type cells expressed alpha-smooth muscle actin, desmin and/or basic-calponin, indicating the plausible smooth muscle cell characteristics of S-type cells. In this study, a new human NB cell line, MP-N-MS, was established from bone marrow metastasis of a one year and six-month old girl with advanced NB, originating from right adrenal gland. Morphology of this cell line is composed of S-type cells. MP-N-MS was identified as a NB cell line by surface membrane antigen analysis and MYCN gene amplification. EWS-FLI1 and EWS-ERG chimeric products, observed in Ewing family tumors, were not detected by RT-PCR (reverse transcriptase-polymerase chain reaction). In cytoskeletal protein analysis, alpha-smooth muscle actin and basic calponin of smooth muscle cell markers were detected. Furthermore, smooth myosin of SM1 isoform was identified in MP-N-MS cell line by immunofluorescence, Western blot and RT-PCR, whereas smooth myosin of SM2 was detected by RT-PCR. MP-N-MS is the first cell line, showing SM1 and SM2 isoforms. The presence of smooth muscle myosin of SM1 and SM2 isoforms in MP-N-MS demonstrated the mature smooth muscle phenotype of this NB cell line, and the ability of NB cells to differentiate into smooth muscle cell.
Sugimoto
T
T
Department of Pediatrics, Miyazaki Medical College, Japan.
Komada
Y
Y
Sakurai
M
M
Horii
Y
Y
Morishita
R
R
Nagai
R
R
Takahashi
K
K
Asada
Y
Y
D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007223:Infant; D009130:Muscle, Smooth / Q000166:cytology; D009218:Myosins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D020033:Protein Isoforms / Q000378:metabolism; D014407:Tumor Cells, Cultured
D020033:Protein Isoforms; D009218:Myosins