title: Tracking adhesion factors in Staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material.
abstract: Ten Staphylococcus caprae strains isolated from four patients and responsible for bone infections following implantation of orthopaedic material were compared to four S. caprae strains collected from milk samples of healthy goats. The following characteristics were investigated: Smal patterns, hybridization patterns with pBA2 (ribotypes), slime production, adhesion to matrix proteins (fibrinogen, fibronectin, collagen) and the staphylococcal adhesion genes (fnbA, clfA, cna, atlE, ica, fbe). None of the characteristics enabled us to distinguish the human strains from the goat strains. Slime was occasionally produced by S. caprae strains but all of them carried nucleotide sequences hybridizing at low stringency with the following genes: atlE encoding a S. epidermidis autolysin binding vitronectin and responsible for the primary adhesion to polystyrene, ica operon involved in the biosynthesis of a S. epidermidis extracellular polysaccharide, and the part of clfA encoding the serine-aspartate repeated region of a S. aureus cell-wall fibrinogen-binding protein.
authors.affiliation: Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
authors.forename: Jeanine
authors.initials: J
authors.lastname: Allignet
authors.affiliation: Service de Microbiologie, Hôpital Beaujon, A.P.-H.P., 92100 Clichy, France.|Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
authors.forename: Jacques-Olivier
authors.initials: JO
authors.lastname: Galdbart
authors.affiliation: Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Morvan
authors.affiliation: Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
authors.forename: Keith G H
authors.initials: KGH
authors.lastname: Dyke
authors.affiliation: Division des Maladies Infectieuses, Hôpital Cantonal Universitaire, Genève, Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Vaudaux
authors.affiliation: Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Aubert
authors.affiliation: Service de Biologie, Hôpital de la Croix-Saint-Simon, 75020 Paris, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Desplaces
authors.affiliation: Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
authors.forename: Névine El
authors.initials: NE
authors.lastname: Solh
mesh_terms: D000818:Animals; D019643:Arthroplasty, Replacement / Q000009:adverse effects*; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001850:Bone Diseases, Infectious / Q000382:microbiology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D006041:Goats; D006801:Humans; D008892:Milk / Q000382:microbiology; D011137:Polystyrenes / Q000378:metabolism; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D011137:Polystyrenes; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific