Proteinase 3 mRNA expression is induced in monocytes but not in neutrophils of patients with cystic fibrosis.
Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls and poorly expressed PR3 mRNA. In contrast, CF monocytes showed significantly higher PR3 concentrations than controls, together with an upregulation of PR3 mRNA expression especially during pulmonary exacerbation. Interestingly, antibiotic treatment fully abrogated PR3 mRNA expression and decreased PR3 protein in monocytes. Our findings highlight a potential role of monocyte-derived PR3 in CF-associated airway inflammation.
Just
J
J
Inserm U 417, Hôpital Saint-Antoine, Bâtiment Raoul Kourilsky, 184 Rue du Faubourg St. Antoine, 75571, Paris Cedex 12, France.
Moog-Lutz
C
C
Houzel-Charavel
A
A
Canteloup
S
S
Grimfeld
A
A
Witko-Sarsat
V
V
Cayre
Y E
YE
D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001424:Bacterial Infections / Q000150:complications / Q000188:drug therapy / Q000201:enzymology*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000150:complications / Q000188:drug therapy / Q000201:enzymology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009000:Monocytes / Q000201:enzymology*; D053146:Myeloblastin; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000187:drug effects; D012016:Reference Values; D012697:Serine Endopeptidases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013183:Sputum / Q000737:chemistry; D015854:Up-Regulation
D000900:Anti-Bacterial Agents; D012333:RNA, Messenger; D009195:Peroxidase; D012697:Serine Endopeptidases; D053146:Myeloblastin