Homocysteine metabolism in cardiovascular cells and tissues: implications for hyperhomocysteinemia and cardiovascular disease.
We have determined the activity and protein levels of CBS in a number of cardiovascular cells and tissues by direct enzyme assay and Western blot analysis, respectively. We have also determined the activity of BHMT in these same tissues and cells and have come to the conclusion that neither enzyme is expressed. This results suggests that in the human cardiovascular system homocysteine metabolism is limited to the remethylation pathway catalyzed by MS. Thus, hyperhomocysteinemia in conjunction with a limited metabolic capacity for homocysteine in the cardiovascular system could result in cellular dysfunction.
Chen
P
P
Cleveland Clinic Foundation, OH, USA.
Poddar
R
R
Tipa
E V
EV
Dibello
P M
PM
Moravec
C D
CD
Robinson
K
K
Green
R
R
Kruger
W D
WD
Garrow
T A
TA
Jacobsen
D W
DW
D000818:Animals; D050940:Betaine-Homocysteine S-Methyltransferase; D001808:Blood Vessels / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood* / Q000209:etiology* / Q000235:genetics; D002319:Cardiovascular System / Q000378:metabolism*; D002478:Cells, Cultured; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism; D006710:Homocysteine / Q000097:blood* / Q000378:metabolism*; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
D012333:RNA, Messenger; D006710:Homocysteine; D008780:Methyltransferases; C494548:BHMT protein, human; D050940:Betaine-Homocysteine S-Methyltransferase; C494550:Bhmt protein, rat; D003541:Cystathionine beta-Synthase