Isolation of a new set of Aspergillus nidulans mutants defective in nuclear migration.
In the filamentous fungus Aspergillus nidulans, nuclear migration in the germ tube is mediated by cytoplasmic dynein. We have previously reported the characterization of four nud (nuclear distribution) genes, nudA, nudC, nudF and nudG, involved in this process. The nudA and nudG genes respectively encode for the heavy chain and the 8-kDa light chain of cytoplasmic dynein. In this work, we describe an improved method for the isolation of nud mutants that has led to the identification of at least ten additional nud loci. We have cloned one of the genes, nudK, and determined that it encodes the actin-related protein Arp1, which is a component of the dynactin complex. This provides the first evidence that dynactin is involved in nuclear migration in A. nidulans.
Xiang
X
X
Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA. XiangXi@RWJA.UMDNJ.EDU
Zuo
W
W
Efimov
V P
VP
Morris
N R
NR
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000502:physiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D000072159:Dynactin Complex; D005800:Genes, Fungal / Q000235:genetics; D005838:Genotype; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000199:Actins; D004271:DNA, Fungal; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins