A toxic extract from sea urchin (T. pileolus) inhibits 45Ca2+ uptake in P2 fraction from chick brain under physiological ionic conditions.
An acidic toxic extract from the sea urchin T. pileolus inhibited time-dependent 45Ca2+ uptake in chick P2 fraction (IC50 about 10 ng/ml). This toxic extract is susceptible to trypsin. The inhibitory effect probably relate to Na+/Ca2+ exchanger.
Zhang
Y
Y
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan.
Wada
T
T
Nakagawa
H
H
Ichida
S
S
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002645:Chickens / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008387:Marine Toxins / Q000737:chemistry / Q000494:pharmacology*; D011489:Protein Denaturation; D012617:Sea Urchins / Q000737:chemistry*; D014020:Tissue Extracts / Q000737:chemistry / Q000494:pharmacology*; D014357:Trypsin
D002132:Calcium Radioisotopes; D008387:Marine Toxins; D014020:Tissue Extracts; D014357:Trypsin; D002118:Calcium