Differential modulation of interleukin-4 and interleukin-13 secretion from human peripheral blood mononuclear cells.
Interleukin-4 (IL-4) and IL-13 which have been implicated in the pathogenesis of atopic reactions elicit many of the same biologic responses. Therefore, time- and stimulus-dependent differences in the regulation of IL-4 and IL-13 production could be of relevance to their biological effects. In this study we tested the hypothesis that stimulation of peripheral blood mononuclear cells (PBMCs) with different inducers of cell activation would result in a differential expression of IL-4 and IL-13. For this purpose, PBMCs of nonatopic volunteers were incubated with phytohaemagglutinin (PHA), phorbolester (PMA), calcium ionophore A23187, or IL-3. The effect of these stimuli on IL-4 and IL-13 production were analysed by enzyme-linked immunoassay (ELISA) in supernatants of cultured PBMCs. Incubation of PBMCs with A23167 and PHA induced both a dose- and time-dependent increase in IL-4 and IL-13 release. A23187 induced concentrations of IL-4 were higher than those of IL-13 whereas IL-4 release following stimulation with PHA was considerably higher for IL-13 compared to IL-4. In contrast, there was a selective increase in IL-13 but not IL-4 concentrations following stimulation of PBMCs with PMA and IL-3 in vitro. In conclusion in this study evidence is provided that IL-4 and IL-13 production are regulated differently which might explain their functional redundancy.
Luttmann
W
W
Department of Pneumology, Medical University Clinics, Freiburg i. Br., Germany. luttmann@pnml.ukl.uni-freiburg.de
Sengler
C
C
Herzog
V
V
Balkow
S
S
Matthys
H
H
Virchow
J C
JC
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D018793:Interleukin-13 / Q000378:metabolism*; D007377:Interleukin-3 / Q000494:pharmacology; D015847:Interleukin-4 / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018793:Interleukin-13; D007377:Interleukin-3; D007476:Ionophores; D010835:Phytohemagglutinins; D015847:Interleukin-4; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium