title: Short-term effects of mineral particle sizes on cellular degradation activity after implantation of injectable calcium phosphate biomaterials and the consequences for bone substitution.
abstract: This in vivo study investigated the influence of two calcium phosphate particle sizes (40-80 microm and 200-500 microm) on the cellular degradation activity associated with the bone substitution process of two injectable bone substitutes (IBS). The tested biomaterials were obtained by associating a biphasic calcium phosphate (BCP) ceramic mineral phase and a 3% aqueous solution of a cellulosic polymer (hydroxypropylmethylcellulose). Both were injected into osseous defects at the distal end of rabbit femurs for 2- and 3-week periods. Quantitative results for tartrate-resistant acid phosphatase (TRAP) cellular activity, new bone formation, and ceramic resorption were studied for statistical purposes. Positive TRAP-stained degradation cells were significantly more numerous for IBS 40-80 than IBS 200-500, regardless of implantation time. BCP degradation was quite marked during the first 2 weeks for IBS 40-80, and bone colonization occurred more extensively for IBS 40-80 than for IBS 200-500. The resorption-bone substitution process occurred earlier and faster for IBS 40-80 than IBS 200-500. Both tested IBS displayed similar biological efficiency, with conserved in vivo bioactivity and bone-filling ability. Differences in calcium phosphate particle sizes influenced cellular degradation activity and ceramic resorption but were compatible with efficient bone substitution.
authors.affiliation: Equipe INSERM Matériaux d'intérêt Biologique, Faculté de Chirurgie Dentaire, Nantes, France. gauthier@vet-nantes.fr
authors.forename: O
authors.initials: O
authors.lastname: Gauthier
authors.forename: J M
authors.initials: JM
authors.lastname: Bouler
authors.forename: P
authors.initials: P
authors.lastname: Weiss
authors.forename: J
authors.initials: J
authors.lastname: Bosco
authors.forename: E
authors.initials: E
authors.lastname: Aguado
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D018786:Bone Substitutes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004577:Electron Probe Microanalysis; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D020007:Implants, Experimental; D007267:Injections; D007527:Isoenzymes / Q000378:metabolism; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008422:Materials Testing; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000378:metabolism*; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology; D010078:Oxazines; D010316:Particle Size; D011817:Rabbits; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D007527:Isoenzymes; D008903:Minerals; D010078:Oxazines; C103718:beta-tricalcium phosphate-monocalcium phosphate monohydrate-calcium sulfate hemihydrate mixture; D008747:Methylcellulose; C071191:MK 458; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D007785:Lactose