A proposed molecular model for the interaction of calcineurin with the cyclosporin A-cyclophilin A complex.
Cyclosporin A (CsA) and FK506 are potent natural product immunosuppressants that induce their biological effects by forming an initial complex with cytosolic proteins termed immunophilins. These drug immunophilin complexes then bind to and inhibit the serine/threonine protein phosphatase calcineurin (CN). Two classes of immunophilin have been identified with cyclophilins (CyP's) being proteins specifically binding CsA and FKBPs specifically binding FK506. Solution and crystal structures of various CsA-CyP and FK506-FKBP complexes have been determined and show no apparent structural similarity between the two classes of drug protein complexes. These findings raise the question as to how, given their structural differences, these two complexes can both inhibit CN. While the crystal structure of the FK506-FKBP12-CN complex has been reported, no structure for a CsA-CyP CN complex has been determined. Here are reported studies that use various modelling strategies to construct a model for the interaction of the cyclosporin A- cyclophilin A complex with calcineurin. The first stage of constructing this model consisted of using conformational comparison of CsA and FK506, GRID and GROUP analysis and restrained molecular dynamics to dock CsA into the FK506 binding site of the FK506-FKBP12-CN structure. An initial model for the CsA-CyPA-CN complex was then constructed by superimposing the structure of the CsA-CyPA complex onto the docked CsA molecule. This model was then optimised with molecular dynamics simulations run on sterically clashing regions. The validity of the model for the CsA-CyPA-CN complex was then examined with respect to the effect of chemical modifications to CsA and amino acid substitutions within CyPA on the ability of the drug-immunophilin complex to inhibit calcineurin.
Ivery
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Department of Chemistry, University of St Andrews, Fife, Scotland, UK. michaeli@pharm.usyd.edu.au
D019943:Amino Acid Substitution; D001665:Binding Sites; D019703:Calcineurin / Q000737:chemistry* / Q000378:metabolism*; D003198:Computer Simulation; D016572:Cyclosporine / Q000737:chemistry / Q000378:metabolism*; D020104:Immunophilins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D019696:Peptidylprolyl Isomerase / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D015203:Reproducibility of Results; D013329:Structure-Activity Relationship; D016559:Tacrolimus / Q000737:chemistry / Q000378:metabolism; D022021:Tacrolimus Binding Proteins
D007166:Immunosuppressive Agents; D016572:Cyclosporine; D019703:Calcineurin; D022021:Tacrolimus Binding Proteins; D020104:Immunophilins; D019696:Peptidylprolyl Isomerase; D016559:Tacrolimus