HLA-G and classical HLA class I transcripts in various components of the adult human eye.
HLA-G transcripts have previously been detected in the ocular cell line HS738, in foetal eyes and in global ocular extract. Using RT-PCR and hybridization, we searched for HLA-G and classical HLA class I transcripts in the corneas of eye donors (n = 6) and in various components of eyes collected at time of surgery: lens (n = 3), iris (n = 2), vitreous body (n = 2) and retina (n = 2). Whereas HLA class I mRNA was abundant in the ocular tissues of all donors, we could not find HLA-G transcript in any ocular tissues obtained from subjects in vivo. The absence of HLA-G in adult eye cornea limits its interest as a possible target in corneal transplantation. Classical HLA class I transcripts were abundant in the cornea and other components of the eye. These results highlight the role of classical HLA class I antigen in eye immunity and corneal transplantation.
Robert
P Y
PY
EP CNRS J0118, Medical University, Limoges, France.
Lasalmonie
C
C
Cogné
M
M
Adenis
J P
JP
Drouet
M
M
D003315:Cornea / Q000276:immunology / Q000378:metabolism; D016039:Corneal Transplantation / Q000276:immunology; D005123:Eye / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics* / Q000378:metabolism*; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D007908:Lens, Crystalline / Q000276:immunology / Q000378:metabolism; D015810:Linkage Disequilibrium; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D006680:HLA Antigens; D015234:HLA-A Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D012313:RNA