Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons.
Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999) 1761-1771] proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated.
Shogomori
H
H
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel.
Burack
M A
MA
Banker
G
G
Futerman
A H
AH
D000818:Animals; D001369:Axons / Q000378:metabolism; D001896:Boron Compounds / Q000378:metabolism; D002454:Cell Differentiation; D016764:Cell Polarity; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000378:metabolism*; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D005732:Gangliosides; C033851:ganglioside, GD1b; D005677:G(M1) Ganglioside; D002772:Cholera Toxin