title: Expression of human herpesvirus-8 (HHV-8) encoded pathogenic genes in Kaposi's sarcoma (KS) primary lesions.
abstract: Transcription of six different HHV-8 specific mRNAs was examined in early- and late-stage KS primary lesions. Expression of the latency-associated T0.7 mRNA and of VP23 mRNA which is a specific marker of lytic/productive infection suggested that HHV-8 is secondarily recruited into the KS lesions by productively infected monocytes, macrophages. From these cells HHV-8 is transmitted to the KS spindle cells, which are latently infected. v-BCL-2, v-MCP-1 and v-IL-6 were not expressed in latently infected KS spindle cells, therefore the impact of these factors in KS pathogenesis appears to be low. By contrast, v-Cyclin D was highly expressed in almost all latently infected spindle cells and may therefore be an important factor triggering progression of late-stage KS lesions.
authors.affiliation: Max Planck Institute for Biochemistry, Department of Virology, Martinsried, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Ascherl
authors.forename: C
authors.initials: C
authors.lastname: Hohenadl
authors.forename: P
authors.initials: P
authors.lastname: Monini
authors.forename: C
authors.initials: C
authors.lastname: Zietz
authors.forename: P J
authors.initials: PJ
authors.lastname: Browning
authors.forename: B
authors.initials: B
authors.lastname: Ensoli
authors.forename: M
authors.initials: M
authors.lastname: Stürzl
mesh_terms: D000368:Aged; D018925:Chemokines / Q000378:metabolism; D056741:Cyclin D; D016213:Cyclins / Q000378:metabolism; D015870:Gene Expression; D005814:Genes, Viral; D015658:HIV Infections / Q000150:complications; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009000:Monocytes / Q000378:metabolism / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012514:Sarcoma, Kaposi / Q000150:complications / Q000378:metabolism / Q000821:virology*; D012878:Skin Neoplasms / Q000150:complications / Q000378:metabolism / Q000821:virology*; D014774:Virulence / Q000235:genetics
chemical_list: D018925:Chemokines; D056741:Cyclin D; D016213:Cyclins; D012333:RNA, Messenger; D012367:RNA, Viral