FB1, a monoclonal antibody reacting with a keratin 14 epitope, stains only a small subset of psoriatic basal keratinocytes.
A murine monoclonal antibody, FB1, reacted with the basal keratinocytes of human stratified epithelia. One-dimensional and two-dimensional immunoblotting assays, performed on keratins extracted from HaCat cells and normal human keratinocytes, showed that FB1 recognizes K14. When LL002, another K14 monoclonal antibody is added, the FB1 stained area in the 2D-immunoblot seems to cover a fraction of the LL002 spot. Immunohistochemical data obtained from studies on normal human tissues supported the K14 specificity of FB1, but when compared with two other monoclonal antibodies, LL002 and RCK107 reacting with K14, some differences appeared. These differences were mainly seen in sweat glands, hair follicles, psoriatic epidermis and salivary glands. In psoriatic epidermis, FB1 showed a heterogeneous pattern of staining of the basal cell compartment. Intense reactivity was only observed at the bottom of the rete ridges. Staining diminished and finally disappeared in the basal cells above the dermal papillae. This observation supports the view that an increased germinative cell population in psoriasis involves a partially differentiated amplifying compartment in which the number of cell divisions is increased.
Parent
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Institute for Interdisciplinary Research in Human and Nuclear Biology, School of Medicine, Free University of Brussels, Belgium.
Golstein
J
J
Mairesse
N
N
Bernerd
F
F
Faverly
D
D
Robben
H
H
Ramaekers
F
F
Heenen
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D053547:Keratin-14; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology; D007633:Keratins / Q000276:immunology / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology; D012016:Reference Values; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000939:Epitopes; C507540:KRT14 protein, human; D053547:Keratin-14; D007633:Keratins