title: Hepatitis A incidence rate estimates from a pilot seroprevalence survey in Rio de Janeiro, Brazil.
abstract: BACKGROUND
  To assess the impact of water sanitation and sewage disposal, part of a major environmental control programme in Rio de Janeiro, we carried out sero-prevalence studies for Hepatitis A virus (HAV) in three micro-regions in Rio de Janeiro. Each region varied with regard to level of sanitation. We are interested in assessing the discriminating power of age-specific prevalence curves for HAV as a proxy for improvement in sanitation. These curves will serve as baseline information to future planned surveys as the sanitation programme progresses.
  
  
  METHODS
  Incidence rate curves from prevalence data are estimated parametrically via a Weibull-like survival function, and non-parametrically via maximum likelihood and monotonic splines. Sera collected from children and adults in the three areas are used to detect antibodies against HAV through ELISA.
  
  
  RESULTS
  We compare baseline incidence curves at the three sites estimated by the three methods. We observe a strong negative correlation between level of sanitation and incidence rates for HAV infection. Incidence estimates yielded by the parametric and non-parametric approaches tend to agree at early ages in the microregion showing the best level of sanitation and to increasingly disagree in the other two.
  
  
  CONCLUSIONS
  Our results support the choice of HAV as a sentinel disease that is associated with level of sanitation. We also introduce monotonic splines as a novel non-parametric approach to estimate incidence from prevalence data. This approach outperforms current estimating procedures.
authors.affiliation: PROCC/Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
authors.forename: C J
authors.initials: CJ
authors.lastname: Struchiner
authors.forename: L M
authors.initials: LM
authors.lastname: de Almeida
authors.forename: R S
authors.initials: RS
authors.lastname: de Azevedo
authors.forename: E
authors.initials: E
authors.lastname: Massad
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006506:Hepatitis A / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D035922:Hepatitis A Antibodies; D017957:Hepatitis A Virus, Human / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000032:analysis*; D006801:Humans; D015994:Incidence; D007223:Infant; D010865:Pilot Projects; D015995:Prevalence; D012189:Retrospective Studies; D012499:Sanitation / Q000379:methods / Q000592:standards; D016036:Seroepidemiologic Studies; D014505:Urban Population
chemical_list: D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies