Molecular cloning and analysis of a pleiotropic regulatory gene locus from the nystatin producer Streptomyces noursei ATCC11455.
A regulatory gene locus from Streptomyces noursei ATCC14455, the producer of the antifungal antibiotic nystatin, was cloned in Streptomyces lividans based on its ability to activate actinorhodin (Act) production in this host. Deletion and DNA sequencing analyses showed that a small gene, designated ssmA, located downstream of an afsR homologue (a known pleiotropic regulator) was responsible for the Act overproduction in S. lividans. Database searches for the ssmA gene product revealed its limited similarity to the AfsR2 regulatory protein from S. lividans and CREA catabolite repressor from Aspergillus nidulans. To study the effect of ssmA on nystatin production, this gene was either deleted from S. noursei genome, or placed under control of PermE* promoter and introduced in S. noursei. The properties of the corresponding strains indicate that ssmA is involved in regulation of growth and antibiotic production only in the media with certain carbon sources.
Sekurova
O
O
UNIGEN Center for Molecular Biology, NTNU, Trondheim, Norway.
Sletta
H
H
Ellingsen
T E
TE
Valla
S
S
Zotchev
S
S
D000595:Amino Acid Sequence; D000880:Anthraquinones / Q000378:metabolism; D000935:Antifungal Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009761:Nystatin / Q000096:biosynthesis*; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D014157:Transcription Factors; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D000935:Antifungal Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009761:Nystatin; D012097:Repressor Proteins; D014157:Transcription Factors; C121757:SsmA protein, Streptomyces noursei; C121758:SsmB protein, Streptomyces noursei; C066581:AfsR protein, Streptomyces coelicolor; C071237:CreA protein, Aspergillus nidulans; C013390:actinorhodin; D000095622:Benzoisochromanequinones