Enhancement of Borna disease virus transcription in persistently infected cells by serum starvation.
Transcription of Borna disease virus (BDV) in persistently infected MDCK (MDCK/BDV) cells increased in the fetal bovine serum free media as detected by Northern blot analysis. Especially, the amount of 1.9-kb RNA without cap formation at the 5' end and polyadenylation at the 3' end, increased as compared to other mRNA molecules of BDV. Growth arrest of MDCK/BDV cells observed in the condition of serum starvation might be important for increasing viral transcription. Since N-cadherin is the responsible factor for cell-to-cell contact, MDCK/BDV cells were cultured in calcium free medium which inhibits the interaction of N-cadherin. However, inhibition of cell-to-cell contact by N-cadherin is not effective on up regulation of viral transcription. Our finding in this study indicates that enhancement of BDV transcription by serum starvation is a useful technique for further investigation in understanding of mechanisms of BDV transcription.
Mizutani
T
T
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Nishi, Sapporo, Japan.
Inagaki
H
H
Hayasaka
D
D
Kariwa
H
H
Takashima
I
I
D000818:Animals; D001891:Borna disease virus / Q000235:genetics*; D015820:Cadherins / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002417:Cattle; D002450:Cell Communication; D002455:Cell Division; D002460:Cell Line; D016895:Culture Media, Serum-Free; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D007668:Kidney; D014158:Transcription, Genetic
D015820:Cadherins; D016895:Culture Media, Serum-Free; D002118:Calcium