title: Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces.
abstract: Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)(12-18)] and oligothymidylic acids [d(pT)(12-24)] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720-1460, USA.
authors.forename: D
authors.initials: D
authors.lastname: Sýkora
authors.forename: F
authors.initials: F
authors.lastname: Svec
authors.forename: J M
authors.initials: JM
authors.lastname: Fréchet
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D009841:Oligonucleotides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009841:Oligonucleotides