title: Characterisation of a polymorphic Tc1-like transposable element of the parasitic nematode Haemonchus contortus.
abstract: Hctc1, a member of the Tc1-family of transposable elements was isolated from the parasitic nematode Haemonchus contortus. Hctc1 is 1590 bp long, is flanked by 55 bp inverted repeats and carries a single open reading frame of a 340 amino acid transposase-like protein. Hctc1 is similar to Tc1 of Caenorhabditis elegans and elements Tcb1 and Tcb2 of Caenorhabditis briggsae in the inverted terminal repeats, the open reading frame, as well as the target insertion sequence. Furthermore, the copy number of Hctc1 is comparable with the Tc1 copy number in low copy strains of C. elegans. The sequence of Hctc1 is highly variable in H. contortus due to deletions, insertions and point mutations, with at least five distinct length variants of Hctc1. Most of the Hctc1 variation was within rather than between H. contortus populations. The high level of sequence variation is probably due to variation generally found for members of the Tc1-family, as well as a high background level of genetic variation of H. contortus.
authors.affiliation: Department of Molecular Recognition, Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands. r.hoekstra@id.dlo.nl
authors.forename: R
authors.initials: R
authors.lastname: Hoekstra
authors.forename: M
authors.initials: M
authors.lastname: Otsen
authors.forename: J A
authors.initials: JA
authors.lastname: Lenstra
authors.forename: M H
authors.initials: MH
authors.lastname: Roos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D018509:DNA, Helminth / Q000032:analysis; D018628:Gene Dosage; D014644:Genetic Variation; D006189:Haemonchus / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements; D018509:DNA, Helminth