Tracking adhesion factors in Staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material.
Ten Staphylococcus caprae strains isolated from four patients and responsible for bone infections following implantation of orthopaedic material were compared to four S. caprae strains collected from milk samples of healthy goats. The following characteristics were investigated: Smal patterns, hybridization patterns with pBA2 (ribotypes), slime production, adhesion to matrix proteins (fibrinogen, fibronectin, collagen) and the staphylococcal adhesion genes (fnbA, clfA, cna, atlE, ica, fbe). None of the characteristics enabled us to distinguish the human strains from the goat strains. Slime was occasionally produced by S. caprae strains but all of them carried nucleotide sequences hybridizing at low stringency with the following genes: atlE encoding a S. epidermidis autolysin binding vitronectin and responsible for the primary adhesion to polystyrene, ica operon involved in the biosynthesis of a S. epidermidis extracellular polysaccharide, and the part of clfA encoding the serine-aspartate repeated region of a S. aureus cell-wall fibrinogen-binding protein.
Allignet
Jeanine
J
Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
Galdbart
Jacques-Olivier
JO
Service de Microbiologie, Hôpital Beaujon, A.P.-H.P., 92100 Clichy, France.|Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
Morvan
Anne
A
Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
Dyke
Keith G H
KGH
Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
Vaudaux
Pierre
P
Division des Maladies Infectieuses, Hôpital Cantonal Universitaire, Genève, Switzerland.
Aubert
Sylvie
S
Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
Desplaces
Nicole
N
Service de Biologie, Hôpital de la Croix-Saint-Simon, 75020 Paris, France.
Solh
Névine El
NE
Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 72724 Paris Cedex 15, France.
D000818:Animals; D019643:Arthroplasty, Replacement / Q000009:adverse effects*; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001850:Bone Diseases, Infectious / Q000382:microbiology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D006041:Goats; D006801:Humans; D008892:Milk / Q000382:microbiology; D011137:Polystyrenes / Q000378:metabolism; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D011137:Polystyrenes; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific