Gene identification by shotgun antisense.
Shotgun antisense is a technique to make a random set of mutant cells or organisms in such a way that one can select an interesting mutant and then sequence part of the mutated gene within a day. In addition to the fantastic rapidity with which one can identify the mutated gene, there are more advantages of this technique over other mutagenesis techniques: (1) one can identify genes that when completely repressed are lethal; (2) one can select which sets of genes will be mutated; and (3) genes that are expressed from multiple copies can be repressed and thus identified.
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Department of Biochemistry and Cell Biology, MS-140, Rice University, 6100 South Main Street, Houston, Texas 77005-1892, USA.
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics; D005804:Genes, Lethal; D005821:Genetic Techniques; D016296:Mutagenesis; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D010957:Plasmids; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense