Extensive alternative splicing in the 5'-untranslated region of the rat and human neuropeptide Y Y5 receptor genes regulates receptor expression.
Neuropeptide Y (NPY) elicits a plethora of physiological effects by interacting with several distinct G protein-coupled receptors. Activation of one of these receptors, the NPY Y5 receptor, is thought to result in increased food intake, anticonvulsant effects, attenuation of opiate withdrawal, inhibition of neuronal activity, and alteration of renal function. Several alternatively spliced human and rat NPY Y5 receptor cDNAs have been isolated that use different combinations of exons in the 5'-untranslated region. The various human NPY Y5 receptor cDNAs appear to be differentially expressed in different brain regions. The level of human NPY Y5 receptor expressed transiently in COS1 cells was significantly influenced by the sequence of the 5'-untranslated region. These results indicate that alternative splicing in the 5'-untranslated region of the human and rat NPY Y5 receptor genes occurs in a tissue-specific manner and is one mechanism by which cells control the level of NPY Y5 receptor expression.
Parker
E M
EM
Department of CNS and Cardiovascular Research, Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA.
Xia
L
L
D020121:5' Untranslated Regions / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011869:Radioligand Assay; D051381:Rats; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020121:5' Untranslated Regions; D018076:DNA, Complementary; D017476:Receptors, Neuropeptide Y; C100568:neuropeptide Y5 receptor