title: Generation of multiple transcripts from the chicken chondromodulin-I gene and their expression during embryonic development.
abstract: Chondromodulin-I (ChM-I) is an angiogenesis inhibitor isolated from fetal bovine cartilage. Here, we report the nucleotide sequence of chicken ChM-I cDNA. Chicken mature ChM-I had a significantly larger N-terminal hydrophilic domain than its mammalian counterparts. Chicken embryos expressed multiple transcripts (3.3, 2.0 and 1.7 kb in size) due to the alternative utilization of polyadenylation signals, whereas only the 1.7 kb transcripts were detected in mammals. Although confined to cartilage and eye at a later stage of development, whole-mount in situ hybridization revealed the expression of ChM-I mRNA in somites, heart, bronchial arches, roof plate, retina and limb buds. The expression pattern of the gene suggests a role for ChM-I in the morphogenesis during embryonic development.
authors.affiliation: Department of Molecular Interaction and Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Japan. shukunam@frontier.kyoto-u.ac.jp
authors.forename: C
authors.initials: C
authors.lastname: Shukunami
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Tanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Hiraki
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002356:Cartilage / Q000196:embryology; D002642:Chick Embryo; D003001:Cloning, Molecular; D005123:Eye / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000235:genetics* / Q000378:metabolism; D006321:Heart / Q000196:embryology; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D018878:Limb Buds / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins