title: The Escherichia coli NadR regulator is endowed with nicotinamide mononucleotide adenylyltransferase activity.
abstract: The first identification and characterization of a catalytic activity associated with NadR protein is reported. A computer-aided search for sequence similarity revealed the presence in NadR of a 29-residue region highly conserved among known nicotinamide mononucleotide adenylyltransferases. The Escherichia coli nadR gene was cloned into a T7-based vector and overexpressed. In addition to functionally specific DNA binding properties, the homogeneous recombinant protein catalyzes NAD synthesis from nicotinamide mononucleotide and ATP.
authors.affiliation: Istituto di Biochimica, Facoltà di Medicina, Università di Ancona, 60131 Ancona, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Raffaelli
authors.forename: T
authors.initials: T
authors.lastname: Lorenzi
authors.forename: P L
authors.initials: PL
authors.lastname: Mariani
authors.forename: M
authors.initials: M
authors.lastname: Emanuelli
authors.forename: A
authors.initials: A
authors.lastname: Amici
authors.forename: S
authors.initials: S
authors.lastname: Ruggieri
authors.forename: G
authors.initials: G
authors.lastname: Magni
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli; D015870:Gene Expression; D008969:Molecular Sequence Data; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C117105:NadR protein, bacteria; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D004247:DNA; D009612:Nicotinamide-Nucleotide Adenylyltransferase