title: A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.
abstract: We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.
authors.affiliation: Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mizuguchi
authors.forename: M A
authors.initials: MA
authors.lastname: Kay
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017498:Adenovirus E1 Proteins / Q000235:genetics*; D017503:Adenovirus E3 Proteins / Q000235:genetics; D017504:Adenovirus E4 Proteins / Q000235:genetics*; D017353:Gene Deletion; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D017498:Adenovirus E1 Proteins; D017503:Adenovirus E3 Proteins; D017504:Adenovirus E4 Proteins; D011994:Recombinant Proteins; D001616:beta-Galactosidase