title: Effects of desensitizing agents on human dentin permeability.
abstract: OBJECTIVE
  To compare the in vitro effects of three desensitizing agents on hydraulic conductance of human dentin: Protect (n = 10), Gluma Desensitizer (n = 10), MS Coat (n = 10) (Pain-Free in the USA).
  
  
  METHODS
  Dentin discs were prepared from 40 freshly extracted normal human third molars. The pulpal side of the dentin discs was etched with 37% phosphoric acid for 15 s and then rinsed under tap water. The coronal side was sequentially ground and the dentin discs were sonicated for 30 min. The hydraulic conductance was measured filtering 20% serum in phosphate buffered saline under a pressure of 15 cm H2O. The hydraulic conductance of each dentin specimen was measured before using the desensitizing agent and this value was designated as 100%. Thirty dentin discs were treated, the hydraulic conductance was remeasured and expressed as a percentage of the hydraulic conductance of that specimen before treatment. The teeth were stored for 1 month at 37 degrees C in deionized water and the hydraulic conductance of the 40 dentin discs was recorded again. Ten dentin discs were left untreated to serve as a control.
  
  
  RESULTS
  No statistical difference was found between the immediate hydraulic conductance of the three groups after treatment. After 1-month storage, the control group showed a statistically higher hydraulic conductance than the three treated groups. There was no statistical difference between the three dentin desensitizing agents evaluated.
authors.affiliation: Laboratoire de Recherche, Unité IMEB, Faculté d'Odontologie, Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Camps
authors.forename: S
authors.initials: S
authors.lastname: Pizant
authors.forename: J
authors.initials: J
authors.lastname: Dejou
authors.forename: J C
authors.initials: JC
authors.lastname: Franquin
mesh_terms: D000134:Acid Etching, Dental; D000704:Analysis of Variance; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D003806:Dentin Permeability / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000188:drug therapy* / Q000517:prevention & control; D019234:Dentinal Fluid / Q000502:physiology; D005976:Glutaral / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology* / Q000627:therapeutic use; D008855:Microscopy, Electron, Scanning; D008963:Molar; D010070:Oxalates / Q000494:pharmacology* / Q000627:therapeutic use; D018709:Statistics, Nonparametric; D013499:Surface Properties
chemical_list: D008689:Methacrylates; D010070:Oxalates; C121034:Pain-Free; C090892:Protect Dentin Desensitizer; C111486:gluma desensitizer; D005976:Glutaral