title: Association between M/L55-polymorphism of paraoxonase enzyme and oxidative DNA damage in patients with type 2 diabetes mellitus and in control subjects.
abstract: The paraoxonase enzyme (PON) gene polymorphism causes a change of methionine (M-allele) to leucine (L-allele). PON may reduce low density lipoprotein oxidation and prevent atherosclerosis. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a sensitive index of oxidative DNA damage. We have studied the association between the PON genotypes and the urinary excretion of 8-OHdG. The study population consisted of 93 Finnish type 2 diabetes patients and 106 non-diabetic control subjects. The 24-h excretion of 8-OHdG was significantly higher in diabetic patients than in control subjects (P < 0.001). In control subjects, the ratio of the 8-OHdG/glomerular filtration rate increased in order of genotype from MM to ML to LL (P < 0.0412). These results suggest that lipid peroxidation may have an effect on DNA oxidation.
authors.affiliation: Department of Clinical Chemistry, Tampere University Hospital, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Malin
authors.forename: V
authors.initials: V
authors.lastname: Rantalaiho
authors.forename: X H
authors.initials: XH
authors.lastname: Huang
authors.forename: O
authors.initials: O
authors.lastname: Wirta
authors.forename: A
authors.initials: A
authors.lastname: Pasternack
authors.forename: J S
authors.initials: JS
authors.lastname: Leinonen
authors.forename: H
authors.initials: H
authors.lastname: Alho
authors.forename: H
authors.initials: H
authors.lastname: Jokela
authors.forename: T
authors.initials: T
authors.lastname: Koivula
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Ochi
authors.forename: S
authors.initials: S
authors.lastname: Toyokuni
authors.forename: T
authors.initials: T
authors.lastname: Lehtimäki
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D043303:Aryldialkylphosphatase; D016022:Case-Control Studies; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004950:Esterases / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004950:Esterases; D043303:Aryldialkylphosphatase; D003849:Deoxyguanosine