title: Cell interactions control the fate of malignant keratinocytes in an organotypic model of early neoplasia.
abstract: The role of cell interactions during early neoplastic progression in human skin is not well understood. We report that the fate and behavior of low-grade malignant cells in stratified epithelium is dependent on their interactions with neighboring cells and with extracellular matrix during the early events in neoplastic progression. We utilized an organotypic tissue model which mimics premalignancy to monitor malignant cells (II-4) genetically marked with beta-gal and grown in the context of either normal human keratinocytes or the immortalized cell line HaCaT. HaCaT cells were permissive for clonal expansion of II-4 cells at ratios of 4:1, 12:1, and 50:1 (HAC:II-4) when compared with coculture with normal human keratinocytes. This II-4 cell expansion was associated with the failure of neighboring HaCaT cells to induce differentiation and cell cycle withdrawal of II-4, as had been seen in the context of normal human keratinocytes. When 12:1 mixtures (NHK:II-4) were stripped of all suprabasal cells and regrown, all beta-gal cells were lost showing that these normal human keratinocyte-suppressed II-4 cells had been actively sorted to a suprabasal position where their clonal expansion was limited. These growth-suppressive effects of normal human keratinocytes were found to be conditional on direct cell-cell contact, as II-4 formed colonies when trypsinized from 12:1 (NHK:II-4) mixtures and grown at clonal density in submerged culture. The distribution and behavior of low-grade malignant cells was therefore dependent on the state of transformation of adjacent keratinocytes and on cell-matrix interactions. These results demonstrate that alterations in the cellular microenvironment are central to the induction of clonal expansion and early neoplastic progression in stratified epithelium.
authors.affiliation: Department of Oral Biology and Pathology, School of Dental Medicine, SUNY at Stony Brook, 11794-8702, USA.
authors.forename: M
authors.initials: M
authors.lastname: Vaccariello
authors.forename: A
authors.initials: A
authors.lastname: Javaherian
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: N E
authors.initials: NE
authors.lastname: Fusenig
authors.forename: J A
authors.initials: JA
authors.lastname: Garlick
mesh_terms: D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000473:pathology*; D012878:Skin Neoplasms / Q000209:etiology*