title: Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration.
abstract: Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.
authors.affiliation: Institute of Medical Biology, Department of Anatomy and Cell Biology, Odense University, Denmark.
authors.forename: S T
authors.initials: ST
authors.lastname: Christensen
authors.forename: J
authors.initials: J
authors.lastname: Chemnitz
authors.forename: E M
authors.initials: EM
authors.lastname: Straarup
authors.forename: K
authors.initials: K
authors.lastname: Kristiansen
authors.forename: D N
authors.initials: DN
authors.lastname: Wheatley
authors.forename: L
authors.initials: L
authors.lastname: Rasmussen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001343:Autophagy / Q000187:drug effects* / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019311:Staurosporine / Q000494:pharmacology*; D016808:Tetrahymena thermophila / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D004791:Enzyme Inhibitors; D019311:Staurosporine