First evidence for the presence of a hydrogenase in the sulfur-reducing bacterium Desulfuromonas acetoxidans.
Hydrogenases, which are ubiquitous in sulfate-reducing bacteria, were previously thought to be absent from Desulfuromonas acetoxidans. For the first time, a hydrogenase from the strict anaerobic sulfur-respiring bacterium D. acetoxidans, grown on ethanol-malate, was detected and enriched. To assay the role of the hydrogenase in the energetic metabolism of D. acetoxidans, we examined the reactivity of the enzyme with polyheme cytochromes from the same bacterium.
Brugna
M
M
Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036 CNRS, Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20, France.
Nitschke
W
W
Toci
R
R
Bruschi
M
M
Giudici-Orticoni
M T
MT
D000595:Amino Acid Sequence; D003574:Cytochrome c Group / Q000378:metabolism; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology; D006864:Hydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D016999:Sulfur-Reducing Bacteria / Q000201:enzymology*
D003574:Cytochrome c Group; C114913:cytochrome C7; D006864:Hydrogenase