Assessment of degree of oxidative stress and antioxidant concentrations in dogs with idiopathic dilated cardiomyopathy.
OBJECTIVE
To assess degree of oxidative stress and antioxidant concentrations in dogs with idiopathic dilated cardiomyopathy (IDCM).


METHODS
Prospective study.


METHODS
18 dogs with IDCM and 16 healthy control dogs.


METHODS
Concentrations of malondialdehyde (an indicator of oxidative stress); vitamins A, C, and E; glutathione peroxidase; and superoxide dismutase were measured.


RESULTS
Glutathione peroxidase concentration was significantly increased in dogs with IDCM, compared with control dogs. Vitamin A and superoxide dismutase concentrations were not significantly different between groups. A negative correlation was found between disease severity and plasma vitamin E concentration. Disease severity was not correlated with concentrations of other antioxidants. Medications did not significantly affect oxidant or antioxidant concentrations.


CONCLUSIONS
The change in glutathione peroxidase concentration and the correlation between vitamin E concentration and disease severity suggest that the oxidant-antioxidant system may play a role in development of IDCM.
Freeman
L M
LM
Department of Clinical Sciences, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
Brown
D J
DJ
Rush
J E
JE
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003124:Colorimetry / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000503:physiopathology*; D004285:Dogs; D004562:Electrocardiography / Q000662:veterinary; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D013482:Superoxide Dismutase / Q000097:blood; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D014801:Vitamin A; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid