CD44 variant isoform CD44v10 expression of human melanoma cell lines is upregulated by hyaluronate and correlates with migration.
CD44, a multifunctional adhesion receptor involved in cell-cell and cell-matrix interactions, plays an important role in the local progression and metastasis of malignant tumours. We investigated relative CD44 variant isoform mRNA expression in six human melanoma cell lines and determined cell migration on hyaluronic acid (HA) coated substrates. Haematopoietic form (CD44H) mRNA expression increased in all melanoma cell lines after plating on HA, whereas the relative CD44 variant exon 10 (CD44v10) mRNA expression increased in only three of the cell lines. Cell migration rates increased on substrates coated with HA in the three CD44v10-positive cell lines, whereas the three CD44v10-negative cell lines showed no modification in migration rates. Immunofluorescent labelling of CD44v10 revealed increased expression with plaques localized to the periphery of cells. Cell lines with increased relative CD44v10 expression exhibited significantly higher mean migration rates on HA. These results indicate that CD44v10 expression functionally relates to melanoma cell migration and suggest that interaction between CD44v10 and HA plays a role in the variable tissue invasion and aggressiveness of different melanoma clones.
Yoshinari
C
C
Department of Dermatology, Iwate Medical University, Morioka, Japan.
Mizusawa
N
N
Byers
H R
HR
Akasaka
T
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D017398:Alternative Splicing; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008545:Melanoma / Q000276:immunology / Q000378:metabolism*; D008957:Models, Genetic; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000276:Adjuvants, Immunologic; C117722:CD44v10 antigen; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D018960:Hyaluronan Receptors; D020033:Protein Isoforms; D012333:RNA, Messenger; D012709:Serum Albumin; D006820:Hyaluronic Acid