title: In vitro effects of peroxynitrite on human spermatozoa.
abstract: In the present study, the in vitro effects of peroxynitrite on sperm motility, lipid peroxidation and sulfhydryl content were examined. Sperm percentage motility and movement characteristics were assessed by a computer-assisted system. Lipid peroxidation was measured by determining malondialdehyde levels using the thiobarbituric acid (TBA) method. Sperm sulfhydryl content was measured by a spectrophotometric method based on reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) by sulfhydryl groups. Percentage motility, movement characteristics and sulfhydryl content decreased significantly in peroxynitrite-treated samples compared to decomposed peroxynitrite-treated samples. Lipid peroxidation in peroxynitrite-treated samples was significantly higher than in decomposed peroxynitrite-treated samples. These results indicate that peroxynitrite anion may cause sperm dysfunction through lipid peroxidation stimulation and total SH depletion.
authors.affiliation: Department of Biochemistry and Urology, Istanbul University, Istanbul Faculty of Medicine, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Oztezcan
authors.forename: U M
authors.initials: UM
authors.lastname: Türkoğlu
authors.forename: E
authors.initials: E
authors.lastname: Kervancioğlu
authors.forename: T
authors.initials: T
authors.lastname: Koçak
authors.forename: N
authors.initials: N
authors.lastname: Koçak-Toker
authors.forename: G
authors.initials: G
authors.lastname: Aykaç-Toker
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism / Q000633:toxicity*; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D009566:Nitrates; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D013481:Superoxides; C039642:peroxynitric acid; D009569:Nitric Oxide; D008315:Malondialdehyde