Evaluation of a new anti-HCV-assay kit for anti-HCV screening in early childhood.
Sera of 20 children falsely identified as positive for hepatitis C virus antibody (Anti-HCV) by a second generation anti-HCV-assay kit (Imucheck-HCV Ab "Kokusai") were re-tested using a new third generation anti-HCV-assay kit (Imucheck x F-HCV C50 Ab "Kokusai"). Seventeen of the samples were reclassified as negative and only three remained positive. Changing well solids in the anti-HCV-assay kit from casein to bovine serum albumin appears to have improved the false-positive rate, most likely as a result of decreased non-specific adsorption of casein antibodies.
Sugiyama
K
K
Department of Pediatrics, Nagoya City University Medical School, Nagoya, Japan. sugiyama@med.nagoya-cu.ac.jp
Goto
K
K
Miyake
Y
Y
Mizutani
F
F
Terabe
K
K
Li
R
R
Maniwa
H
H
Kawabe
Y
Y
Wada
Y
Y
D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D008403:Mass Screening; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic