Hydrochloric acid treatment for rapid recovery of Shiga toxin-producing Escherichia coli O26, O111 and O157 from faeces, food and environmental samples.
We developed a hydrochloric acid treatment for the isolation of Shiga toxin-producing Escherichia coli (STEC) O26:H11, O111:H- and O157:H7 strains from a variety of samples. After exposure to an equal volume of 1/8N HCl solution for 30 sec, the fecal suspensions and enrichment cultures were spread onto cefixime-tellurite-sorbitol-MacConkey (CT-SMAC) agar. This HCl treatment increased the sensitivity for detection of STEC O26:H11, O111:H- and O157:H7 strains and decreased the growth of other microorganisms, from faecal samples and enrichment cultures of a variety of samples. This approach is an important economical and time-saving method to simplify and speed up isolation of STEC O26:H11, O111:H- and O157:H7 from a variety of samples.
Fukushima
H
H
Public Health Institute of Shimane Prefecture, Japan. hiroshi@joho-shimane.or.jp
Gomyoda
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D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology*; D022603:Shiga Toxins
D001427:Bacterial Toxins; D022603:Shiga Toxins; D006851:Hydrochloric Acid