High-mannose type oligosaccharide-dependent apoptosis in U937 cells induced by pradimicin, a mannose-binding antibiotic.
Cell surface oligosaccharides play a role in a variety of biological events such as cell adhesion and signal transduction. We have shown that BMY-28864, a semi-synthetic analog of pradimicin, induced apoptosis of U937 cells which had been incubated with 1-deoxymannojirimycin, an inhibitor of mannosidase I. BMY-28864 was not cytotoxic to the cells which had been cultivated with other glycosidase inhibitors such as castanospermine and swainsonine. We thus propose that BMY-28864 induces apoptosis by acting on a specific mannose-rich oligosaccharide, presumably (Man)9(GlcNAc)2+.
Oki
T
T
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan.
Yamazaki
Y
Y
Nomura
N
N
Furumai
T
T
Igarashi
Y
Y
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology*; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005959:Glucosidases / Q000037:antagonists & inhibitors; D006801:Humans; D007212:Indolizines / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism*; D008361:Mannosidases / Q000037:antagonists & inhibitors; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D017026:Swainsonine / Q000494:pharmacology; D020298:U937 Cells; D000095643:Pradimicins and Benanomicins; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D016650:Fluorescein-5-isothiocyanate; D005959:Glucosidases; D007212:Indolizines; D008358:Mannose; D008361:Mannosidases; D009844:Oligosaccharides; D017026:Swainsonine; C067714:N,N-dimethylpradimicin FA-2; C066544:pradimicin FA 2; D000095643:Pradimicins and Benanomicins; C046090:mannosyl-oligosaccharide 1,2-alpha-mannosidase; C037806:castanospermine; D054836:Indolizidines