title: Change of liver function in hypertrophying lobe of rabbit liver after portal branch ligation.
abstract: Preoperative portal embolization (PE) is useful for the prevention of postoperative liver failure after extended hepatectomy. However, clinical evaluation of liver function in the hypertrophying lobe after PE has not been studied. Here we report functional changes in the hypertrophying lobe using a 80% portal-branch-ligation rabbit model. Liver function was evaluated by the expression of liver-specific genes detected by Northern blot analysis and plasma disappearance rate of indocyanine green (ICG). The weight of the unligated lobe after portal ligation increased about twofold on the 7th postoperative day (POD) and about threefold on the 14th POD. The mRNA levels of the liver-specific genes (albumin, aldolase B, and tyrosine aminotransferase) in the unligated lobe decreased to about 50% on the 1st POD and returned to the preoperative levels on the 7-14th POD. In contrast, the expression of histone H2B mRNA increased on the 3rd-7th POD. The plasma disappearance rate of ICG (K-ICG) in the rabbit that has only the unligated lobe did not significantly change during the first 7 days, but then improved and recovered to 80% of that in the rabbit that has whole liver on the 14th POD. These results indicate that liver function of the hypertrophying lobe after portal branch ligation does not increase during the first 7 days despite an increase in liver weight. This finding suggests that the compensatory hypertrophying liver is enlarging without functional augmentation in the early period after PE.
authors.affiliation: First Department of Surgery, Yamagata University, Yamagata, 990-9585, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Urayama
authors.forename: S
authors.initials: S
authors.lastname: Ishiyama
authors.forename: T
authors.initials: T
authors.lastname: Kuzumaki
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: A
authors.initials: A
authors.lastname: Fuse
authors.forename: H
authors.initials: H
authors.lastname: Kuzu
authors.forename: Y
authors.initials: Y
authors.lastname: Igarashi
authors.forename: K
authors.initials: K
authors.lastname: Suto
authors.forename: M
authors.initials: M
authors.lastname: Tsukamoto
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000493:pharmacokinetics; D004247:DNA / Q000378:metabolism; D006128:Growth / Q000502:physiology; D006984:Hypertrophy; D007208:Indocyanine Green / Q000493:pharmacokinetics; D008026:Ligation; D008099:Liver / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D009929:Organ Size; D011168:Portal System / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
chemical_list: D004396:Coloring Agents; D012333:RNA, Messenger; D004247:DNA; D007208:Indocyanine Green