Association between M/L55-polymorphism of paraoxonase enzyme and oxidative DNA damage in patients with type 2 diabetes mellitus and in control subjects.
The paraoxonase enzyme (PON) gene polymorphism causes a change of methionine (M-allele) to leucine (L-allele). PON may reduce low density lipoprotein oxidation and prevent atherosclerosis. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a sensitive index of oxidative DNA damage. We have studied the association between the PON genotypes and the urinary excretion of 8-OHdG. The study population consisted of 93 Finnish type 2 diabetes patients and 106 non-diabetic control subjects. The 24-h excretion of 8-OHdG was significantly higher in diabetic patients than in control subjects (P < 0.001). In control subjects, the ratio of the 8-OHdG/glomerular filtration rate increased in order of genotype from MM to ML to LL (P < 0.0412). These results suggest that lipid peroxidation may have an effect on DNA oxidation.
Malin
R
R
Department of Clinical Chemistry, Tampere University Hospital, Finland.
Rantalaiho
V
V
Huang
X H
XH
Wirta
O
O
Pasternack
A
A
Leinonen
J S
JS
Alho
H
H
Jokela
H
H
Koivula
T
T
Tanaka
T
T
Okada
K
K
Ochi
H
H
Toyokuni
S
S
Lehtimäki
T
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D043303:Aryldialkylphosphatase; D016022:Case-Control Studies; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004950:Esterases / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D011110:Polymorphism, Genetic
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004950:Esterases; D043303:Aryldialkylphosphatase; D003849:Deoxyguanosine