DNA typing for identification of some species of Calliphoridae. An interest in forensic entomology.
To determine precisely post mortem interval, larvae and puparium species found on a corpse have to be identified. Among more than 200 cases examined at the entomology department of the Institut de Recherche Criminelle de la Gendarmerie Nationale, two-thirds concerned corpses less than one month old. Therefore, insects from first and second screwworms are the most frequently found [1]. Some species commonly found in France, such as different Lucilia and Calliphora vicina Robineau-Desvoidy, are easily identifiable at an adult stage, but are almost impossible to differentiate at immature stages when only fragments of puparium or necrosed first instar larvae are available. For this reason, an easy and objective method of identification was thus searched by genetic analysis of these insects. Sequencing of partial gene of sub unit I of cytochrome oxydase has been used to predict restriction sites. Restriction enzyme cleavage of PCR products with Dde I allowed us to differentiate these species.
Malgorn
Y
Y
Institut de Recherche Criminelle de la Gendarmerie Nationale, Rosny-Sous-Bois, France.
Coquoz
R
R
D000367:Age Factors; D000818:Animals; D001344:Autopsy; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000235:genetics* / Q000502:physiology; D005260:Female; D005602:France; D006801:Humans; D007814:Larva / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D010058:Oviposition / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods*; D013045:Species Specificity; D013997:Time Factors
D004272:DNA, Mitochondrial