title: Infection with GB virus C/hepatitis G virus among blood donors and hemophiliacs in Martinique, a Caribbean island.
abstract: GB virus C/hepatitis G virus (GBV-C/HGV) RNA was detected by reverse transcription-polymer- ase chain reaction with primers derived from the nonstructural region 3 (NS3) in 9 (4.1%) of 221 blood donors and 2 of 20 (10%) hemophilia patients in Martinique, French West Indies. Anti-E2 antibodies were found in sera from 33 (14.9%) of the blood donors and 5 (25%) of the hemophiliacs. None of the subjects was positive for both GBV-C/HGV RNA and anti-E2. Among the 20 hemophiliacs, 12 (60%) had anti-HCV antibodies and 7 (35%) were positive for HCV RNA by PCR. All patients positive for HCV markers belonged to the group of 13 patients exposed previously to blood factor concentrates that were not activated virally. Nucleotide sequences of the 5'-untranslated region (5'UTR) of the GBV-C/HGV genome were obtained for the 10 NS3 PCR positive samples. Phylogenetic comparison of these isolates with reference isolates published previously showed a strong homology with European and American GBV-C/HGV strains, 8 isolates belonging to the genotype 2a and 1 isolate to the type 2b. The isolate from 1 blood donor was identified as subtype 1a, indicating the presence of West African type strains.
authors.affiliation: Etablissement de Transfusion Sanguine, Centre Hospitalier Universitaire, Fort-de-France, Martinique (FWI).
authors.forename: R
authors.initials: R
authors.lastname: Césaire
authors.forename: J
authors.initials: J
authors.lastname: Martial
authors.forename: H
authors.initials: H
authors.lastname: Maier
authors.forename: B
authors.initials: B
authors.lastname: Kerob-Bauchet
authors.forename: O
authors.initials: O
authors.lastname: Bera
authors.forename: E
authors.initials: E
authors.lastname: Duchaud
authors.forename: A
authors.initials: A
authors.lastname: Brebion
authors.forename: S
authors.initials: S
authors.lastname: Pierre-Louis
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors / Q000706:statistics & numerical data*; D005260:Female; D018067:Flaviviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005838:Genotype; D006467:Hemophilia A / Q000097:blood / Q000821:virology*; D016174:Hepacivirus / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000097:blood; D006525:Hepatitis, Viral, Human / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008395:Martinique / Q000453:epidemiology; D008875:Middle Aged; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood
chemical_list: D020121:5' Untranslated Regions; D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D012367:RNA, Viral