Intragenic recombinants of Plasmodium falciparum identified by in situ polymerase chain reaction.
We report an in situ PCR technique for visualising amplified DNA of blood forms of Plasmodium falciparum on microscope slides by fluorescence microscopy. The method is used to assess the changes in frequency of different alleles of the MSP1 gene in cultures of the progeny of a cross. We show that parasites with a recombinant form of this protein possess an initial growth advantage before declining in numbers over the long-term.
Ranford-Cartwright
L
L
Institute of Cell, Animal and Population Biology, University of Edinburgh, UK. 1.ranford-cartwright@ed.ac.uk
Walliker
D
D
D000483:Alleles; D000818:Animals; D005787:Gene Frequency; D017125:Genes, Protozoan; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic
D020066:Merozoite Surface Protein 1