[Construction of human genomic YAC clones with "terminal modification"].
A human YAC genomic pool with more than 4,000 clones was constructed by means of "terminal modification" of YAC vector and genomic inserts, as well as optimization of relevant experimental parameters. The transformation rate is approximately 400-500 transformants per microgram DNA. Preliminary characterization of randomly sampled clones demonstrates that YAC-specific inserts, in the average size of 450 kb and ranging 150-750 kb, were detected from 80% of the clones. The vector ligation experiment indicates the efficient reduction of "coligation" by terminal modification.
Zhang
X
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai.
Yu
L
L
Deng
Y
Y
Liu
G
G
Yang
Y
Y
D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D015894:Genome, Human; D006801:Humans; D008297:Male; D011897:Random Allocation; D014170:Transformation, Genetic
D004247:DNA