Sphingomyelin inhibits platelet 12-lipoxygenase activity.
The effect of sphingomyelin on the formation of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), thromboxane B2 and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) in washed rabbit platelets was examined. Sphingomyelin had a powerful inhibitory effect on 12-HETE formation, while it produced only a small increase in thromboxane B2 and HHT formation. The sphingomyelin metabolite ceramide did not affect the formation of 12-HETE, thromboxane B2 and HHT. These results suggest that sphingomyelin is a selective inhibitor of platelet 12-lipoxygenase and may have functional effects in platelets.
Fujimoto
Y
Y
Department of Hygenic Chemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan.
Sakuma
S
S
Tsunomori
M
M
Sumiya
T
T
Fujita
T
T
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology*; D002518:Ceramides / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D013109:Sphingomyelins / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis
D002518:Ceramides; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D013109:Sphingomyelins; D016718:Arachidonic Acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D013929:Thromboxane B2; D001092:Arachidonate 12-Lipoxygenase