Validation of a rapid method for extraction of total RNA applied to Leishmania promastigotes.
This report explains a rapid procedure (approximately 50 min) for the isolation of highly purified total RNA from Leishmania promastigotes based on extraction with acidic phenol. The simplicity of the manipulations required make this method ideal for processing multiple samples; the quality of the RNA obtained is suitable for reverse transcription polymerase chain reaction analysis.
Pérez
J A
JA
Departamento de Parasitología, Facultad de Farmacia, Universidad de La Laguna, Spain.
Aragón
Z
Z
Piñero
J E
JE
del Castillo
A
A
de Armas
F
F
Valladares
B
B
D000818:Animals; D007891:Leishmania; D016053:RNA, Protozoan / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016053:RNA, Protozoan