title: Adherence of Pseudomonas aeruginosa to inanimate polymers including biomaterials.
abstract: Cells of Pseudomonas aeruginosa were adhered to polymethyl methacrylate, polyvinyl acetate, polyvinyl chloride, polyhydroxyethyl methacrylate, mixed-acrylic, silicone, and natural latex materials. Planktonic bacteria and bacteria that adhered to the test materials were compared for their uptake of either L-[3,4,5-3H] leucine or [methyl-3H] thymidine during growth in a minimal medium. Leucine incorporation was reduced and thymidine uptake was negligible in adherent bacteria for up to 8 h following primary attachment by which time cells in the planktonic state showed active uptake of both substrates. These reduced uptake periods correlated with lag phases of growth of adherent cells as determined with a sonication-release plate count procedure and analyses of adenosine triphosphate (ATP). The extent of the lag phase of the adherent populations was dependent on initial densities of adhered cells and the nature of the substratum.
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia 30302-4010, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Stone
authors.forename: M M
authors.initials: MM
authors.lastname: Gabriel
authors.forename: D G
authors.initials: DG
authors.lastname: Ahearn
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000096:biosynthesis; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D015169:Colony Count, Microbial; D007930:Leucine / Q000378:metabolism; D011108:Polymers / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D000255:Adenosine Triphosphate; D007930:Leucine; D013936:Thymidine