title: Discrimination between endemic and feedborne Salmonella Infantis infection in cattle by molecular typing.
abstract: Salmonella enterica serovar Infantis is endemic in Finnish cattle. Feed contaminated with S. Infantis was distributed to cattle farms in May 1995. Following increased sampling, S. Infantis was detected on 242 farms in 1995. Molecular typing was used to differentiate the farms that were infected by the feed-related Infantis from those infected by other endemic strains. Twenty-three isolates from feed in 1995 and 413 from cattle (72 from 19924, 324 from 1995, 17 from 1996-7) were analysed. The feed-related Infantis was clonally related to the endemic infection by the ribotype, IS200-type and XbaI-profile. The feed isolates had a distinctive plasmid that appeared in pulsed-field gel electrophoresis as a 60 kb band when cleaved with XbaI or linearized by S1-nuclease. This plasmid appeared in cattle only since the outbreak and seemed stable on the follow-up farms. In addition to contact farms, the feedborne strain was found on 19% of the farms infected with S. Infantis in 1995 but not having bought suspected feedstuffs, possibly as secondary infections.
authors.affiliation: National Veterinary and Food Research Institute, Kuopio Regional Laboratory, Finland.
authors.forename: N
authors.initials: N
authors.lastname: Lindqvist
authors.forename: S
authors.initials: S
authors.lastname: Heinikainen
authors.forename: A M
authors.initials: AM
authors.lastname: Toivonen
authors.forename: S
authors.initials: S
authors.lastname: Pelkonen
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000209:etiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D019353:Endemic Diseases / Q000662:veterinary*; D005387:Finland / Q000453:epidemiology; D005516:Food Microbiology; D015994:Incidence; D010957:Plasmids / Q000737:chemistry; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000209:etiology; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal