title: Enantioseparations with the macrocyclic antibiotic ristocetin A using a countercurrent process in CE.
abstract: The chiral selectivity of ristocetin A was examined in a countercurrent process in CE using a coated column to suppress electroosmotic flow. Excellent enantioseparations of several nonsteroidal antiinflammatories, dansylamino acids, dinitrophenyl-derivatized amino acids, and other optically active compounds were achieved. The chiral selectivity of ristocetin A also was examined as a function of antibiotic concentration and pH. Enantioresolution was found to significantly improve with a slight increase in migration time upon increasing chiral selector concentration. Enantioselectivities were found to be greatly influenced by pH of the running buffer.
authors.affiliation: Department of Chemistry, Millsaps College, 1701 N. State Street, Jackson, Mississippi 39210, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Oswald
authors.forename: T J
authors.initials: TJ
authors.lastname: Ward
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D003378:Counterimmunoelectrophoresis; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D012310:Ristocetin / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000900:Anti-Bacterial Agents; D012310:Ristocetin; C016719:ristocetin A