title: Analysis of a 26-kb region linked to the Mhc in zebrafish: genomic organization of the proteasome component beta/transporter associated with antigen processing-2 gene cluster and identification of five new proteasome beta subunit genes.
abstract: Sequencing of zebrafish (Danio rerio) bacterial artificial chromosome and P1 artificial chromosome genomic clone fragments and of cDNA clones has led to the identification of five new loci coding for beta subunits of proteasomes (PSMB). Together with the four genes identified previously, nine PSMB genes have now been defined in the zebrafish. Six of the nine genes reside in the zebrafish MHC (Mhc) class I region, four of them reside in a single cluster closely associated with TAP2 on a 26-kb long genomic fragment, and two reside at some distance from the fragment. In addition to homologues of the human genes PSMB5 through PSMB9, two new genes, PSMB11 and PSMB12, have been found for which there are no known corresponding genes in humans. The new genes reside in the PSMB cluster in the Mhc. Homology and promoter region analysis suggest that the Mhc-associated genes might be inducible by IFN-gamma. The zebrafish class I region contains representatives of three phylogenetically distinguishable groups of PSMB genes, X, Y, and Z. It is proposed that these genes were present in the ancestral PSMB region before Mhc class I genes became associated with it.
authors.affiliation: Max-Planck-Institut für Biologie, Abt. Immungenetik, Tübingen, Germany.
authors.forename: B W
authors.initials: BW
authors.lastname: Murray
authors.forename: H
authors.initials: H
authors.lastname: Sültmann
authors.forename: J
authors.initials: J
authors.lastname: Klein
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics*; D001482:Base Composition / Q000276:immunology; D001483:Base Sequence; D020451:Contig Mapping; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005091:Exons / Q000276:immunology; D007438:Introns / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D005810:Multigene Family / Q000276:immunology*; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000276:immunology; D046988:Proteasome Endopeptidase Complex; D015027:Zebrafish / Q000235:genetics* / Q000276:immunology*; D029961:Zebrafish Proteins
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D009097:Multienzyme Complexes; C121057:Psmb11 protein, zebrafish; C121061:Psmb9A protein, zebrafish; D029961:Zebrafish Proteins; C121058:psmb10 protein, zebrafish; C489513:tap2t protein, zebrafish; C071692:TAP2 protein, human; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C121055:Psmb7 protein, zebrafish