title: Aberrant wound healing and TGF-beta production in the autoimmune-prone MRL/+ mouse.
abstract: Wound healing is a complex process that involves inflammation, apoptosis, growth, and tissue remodeling. The autoimmune-prone inbred mouse strain MRL/+ manifests accelerated and extensive healing to ear punch wounds, suggesting a link between immune defects and wound healing. Prior studies with lupus-prone mice have shown that hematopoietic cells of lupus-prone strains can transfer disease to otherwise non-autoimmune-prone recipients. In this study we performed reciprocal bone marrow transfers between MRL and the control strain B10.BR and found that radioresistant MRL/+ host cells, rather than hematopoietic cells, are required for the healing response. We have also made the novel observations that, compared to normal controls, MRL/+ hematopoietic cells overproduce TGF-beta1 and manifest impaired inflammatory responses to lipopolysaccharide challenge. These features suggest that the aberrant wound healing phenotype of MRL mice is independent of their propensity to develop autoimmunity.
authors.affiliation: Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado, 80206, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Kench
authors.forename: D M
authors.initials: DM
authors.lastname: Russell
authors.forename: V A
authors.initials: VA
authors.lastname: Fadok
authors.forename: S K
authors.initials: SK
authors.lastname: Young
authors.forename: G S
authors.initials: GS
authors.lastname: Worthen
authors.forename: J
authors.initials: J
authors.lastname: Jones-Carson
authors.forename: J E
authors.initials: JE
authors.lastname: Henson
authors.forename: P M
authors.initials: PM
authors.lastname: Henson
authors.forename: D
authors.initials: D
authors.lastname: Nemazee
mesh_terms: D000375:Aging / Q000235:genetics / Q000502:physiology; D000818:Animals; D016026:Bone Marrow Transplantation; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D019463:Mice, Inbred MRL lpr / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology; D011014:Pneumonia / Q000503:physiopathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D018183:Transplantation Chimera; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
chemical_list: D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016212:Transforming Growth Factor beta