Postexposure immunoprophylaxis of primary isolates by an antibody to HIV receptor complex.
mAb B4 is a monoclonal antibody directed against HIV receptor complex. The antibody had broad neutralizing activity against HIV and provided postexposure prophylaxis to hu-peripheral blood leukocyte (PBL)-severe combined immunodeficient mice and chimpanzees. B4 recognized a complex receptor site for HIV on the T cell surface that includes CD4 and also may be influenced by interaction with HIV coreceptors. mAb B4 preferentially neutralized primary HIV-1 isolates compared with T cell line-adapted strains, including syncytium-inducing and non-syncytium-inducing phenotypes, representatives from HIV-1 subtypes A-G, as well as HIV-2, simian immunodeficiency virus, and chimeric simian/human immunodeficiency virus (SHIV). Neutralization was demonstrated in both pre- and postinfection models. The administration of mAb B4 after infectious challenge totally interrupted the infection of hu-PBL-severe combined immunodeficient mice by PBL-grown HIV-1 and the infection of chimpanzees by chimp-adapted HIV-1. This mode of protection suggested that the anti-HIV receptor antibody is efficacious for prophylaxis after exposure to HIV and for prevention of maternal transmission and may be an effective antiretroviral agent for treatment.
Wang
C Y
CY
United Biomedical Inc., 25 Davids Drive, Hauppauge, NY 11788, USA. pr@unitedbiomedical.com
Sawyer
L S
LS
Murthy
K K
KK
Fang
X
X
Walfield
A M
AM
Ye
J
J
Wang
J J
JJ
Chen
P D
PD
Li
M L
ML
Salas
M T
MT
Shen
M
M
Gauduin
M C
MC
Boyle
R W
RW
Koup
R A
RA
Montefiori
D C
DC
Mascola
J R
JR
Koff
W C
WC
Hanson
C V
CV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology* / Q000627:therapeutic use; D015704:CD4 Antigens / Q000276:immunology*; D015497:HIV-1 / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D018451:Homosexuality, Male; D006801:Humans; D007167:Immunotherapy; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009500:Neutralization Tests; D002679:Pan troglodytes; D015728:Receptors, HIV / Q000276:immunology*; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015728:Receptors, HIV