Simultaneous determination of anions and cations by ion-exclusion chromatography-cation-exchange chromatography with tartaric acid/18-crown-6 as eluent.
Ion-exclusion chromatography-cation-exchange chromatography was developed for the simultaneous separation of common inorganic anions and cations (Cl-, NO3- and SO4(2-); Na+, NH4+, K+, Mg2+ and Ca2+) on a weakly acidic cation-exchange column by elution with weak acid. Generally, the resolution among these monovalent cations was only moderate, thereby hindering the determination of these analytes in natural-water samples. Therefore, 18-crown-6 was added to the eluent to improve the resolution. A good separation of these anions and cations on a weakly acidic cation-exchange column was achieved in 30 min by elution with 5 mM tartaric acid/6 mM 18-crown-6/methanol-water (7.5:92.5). The ion-exclusion chromatography-cation-exchange chromatography method developed here was successfully applied to the separation of major anions and cations in an environmental water sample.
Kwon
S M
SM
Taegu City Health and Environmental Research Institute, South Korea.
Lee
K P
KP
Tanaka
K
K
Ohta
K
K
D000838:Anions / Q000032:analysis*; D002412:Cations / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D043844:Crown Ethers; D004988:Ethers, Cyclic / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D011891:Rain; D013644:Tartrates / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000838:Anions; D002412:Cations; D043844:Crown Ethers; D004988:Ethers, Cyclic; D013644:Tartrates; D014867:Water; C015762:18-crown-6; C029768:tartaric acid; D000432:Methanol