title: Role of the sporulation protein BofA in regulating activation of the Bacillus subtilis developmental transcription factor sigmaK.
abstract: During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro-sigmaK to sigmaK. The results show that in the presence of BofA, SpoIVFA levels increase by greater than 20-fold, SpoIVFA is substantially stabilized, and pro-sigmaK processing is inhibited. In addition, enhanced accumulation of the SpoIVFA protein in the absence of BofA (achieved through the use of an ftsH null mutation) substantially inhibits pro-sigmaK processing. These results suggest that during growth, increased accumulation of the SpoIVFA protein inhibits the activity of SpoIVFB-GFP and regulates the activation of sigmaK.
authors.affiliation: Section on Microbial Genetics, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2785, USA. resnekov@biosun.harvard.edu
authors.forename: O
authors.initials: O
authors.lastname: Resnekov
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins; D012808:Sigma Factor / Q000378:metabolism*; D012965:Sodium Chloride; D013171:Spores, Bacterial
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011498:Protein Precursors; D012097:Repressor Proteins; D012808:Sigma Factor; C074423:bofA protein, Bacillus subtilis; C071441:spoIVFA protein, Bacillus subtilis; D012965:Sodium Chloride; D000251:Adenosine Triphosphatases