Migration of exogenous immature hematopoietic cells into adult mouse brain parenchyma under GFP-expressing bone marrow chimera.
Bone marrow transplantation with GFP-expressing cells from GFP-transgenic mice resulted in migration of GFP-positive cells into peripheral tissues and brain parenchyma. Most of these cells were observed as colony-like clusters. GFP-positive clusters in the brain were stained by antibody for ER-MP12, but those in the peripheral tissues were not. Since ER-MP12 antigen has been reported as a marker for murine early-stage myeloid precursor, this might suggest that some parts of phagocytic cells in the brain parenchyma such as microglia are derived from undifferentiated pluripotent hematopoietic cells.
Ono
K
K
Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho, Nagoya, Aichi, 467-8603, Japan.
Takii
T
T
Onozaki
K
K
Ikawa
M
M
Okabe
M
M
Sawada
M
M
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D016026:Bone Marrow Transplantation / Q000502:physiology*; D001921:Brain / Q000166:cytology*; D002465:Cell Movement; D005434:Flow Cytometry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D008099:Liver / Q000166:cytology; D008164:Luminescent Proteins / Q000235:genetics*; D008168:Lung / Q000166:cytology; D008297:Male; D008636:Mesencephalon / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology; D018183:Transplantation Chimera
D015415:Biomarkers; D008164:Luminescent Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D049452:Green Fluorescent Proteins