Co-introduction of an antisense gene for an endogenous seed storage protein can increase expression of a transgene in Arabidopsis thaliana seeds.
We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and a 2S albumin antisense gene contained reduced amounts of 2S albumins and increased arcelin-5 (Arc5) accumulation levels compared to lines harboring the arc5-I gene only. Arc5 production could be enhanced to more than 24% of the total seed protein content, suggesting that antisense technology could be of great utility to favor high expression of transgenes.
Goossens
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Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.
Van Montagu
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Angenon
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G
D055516:2S Albumins, Plant; D000418:Albumins / Q000235:genetics*; D052179:Antigens, Plant; D017360:Arabidopsis / Q000235:genetics*; D016373:DNA, Antisense / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics*; D012639:Seeds / Q000235:genetics*; D014170:Transformation, Genetic
D055516:2S Albumins, Plant; D000418:Albumins; D052179:Antigens, Plant; D016373:DNA, Antisense; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D010940:Plant Proteins; D011498:Protein Precursors; C090486:arcelin 5 protein, Phaesolus vulgaris