title: Survival of axotomized retinal ganglion cells in peripheral nerve-grafted ferrets.
abstract: OBJECTIVE
  Peripheral nerve (PN) grafting to the optic nerve stump stimulates not only axonal regeneration of the axotomized retinal ganglion cells (RGCs) into the grafted PN but also their survival. The purpose of the present study was to determine the number, distribution, and soma diameter of only surviving RGCs without regenerated axons and surviving RGCs with regenerated axons in PN-grafted mammals.
  
  
  METHODS
  A segment of PN was grafted to the optic nerve stump of adult ferrets. Two months after the PN grafting, surviving RGCs with regenerated axons were retrogradely labeled with granular blue (GB) and stained with RGC-specific antibody C38. Surviving RGCs without regenerated axons were identified as C38-positive cells without GB labeling.
  
  
  RESULTS
  Twenty-one percent of RGCs survived axotomy after PN grafting in the area centralis (AC), whereas 47% survived in the peripheral retina. Twenty-six percent of surviving RGCs in the AC exhibited axonal regeneration, which was higher than that in the peripheral retina. Soma diameter histograms revealed that RGCs with regenerated axons showing both GB and C38 positivity were in the large soma diameter ranges. In contrast, the soma diameter distribution of surviving RGCs that did not have regenerated axons showed a peak in the smaller soma diameter ranges.
  
  
  CONCLUSIONS
  The present data suggest that PN grafting promotes survival of axotomized RGCs more effectively in the peripheral retina than in the AC. Among surviving RGCs, the larger cells exhibited axonal regeneration into the grafted PN, whereas the axons of smaller cells did not to regenerate in either the AC or the peripheral retina.
authors.affiliation: Department of Physiology, Osaka University Medical School, Japan.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Quan
authors.forename: J
authors.initials: J
authors.lastname: Kosaka
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019771:Axotomy; D015415:Biomarkers; D002452:Cell Count; D002470:Cell Survival; D005289:Ferrets; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D008297:Male; D009416:Nerve Regeneration; D009900:Optic Nerve / Q000502:physiology* / Q000601:surgery; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D012584:Sciatic Nerve / Q000637:transplantation*
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D007211:Indoles; C043991:granular blue