Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species.
The complete nucleotide sequence of RNA-2 from a Japanese isolate IP of broad bean wilt virus (BBWV) was determined. The sequence encodes a single large polyprotein, which contains a putative movement protein and two coat proteins (CPs). The 3'-terminal sequences of RNA-2 were also determined for three other Japanese isolates and two ATCC isolates (PV132 and PV176) of BBWV. The CPs of the four Japanese isolates share 86.8-98.0% amino acid sequences homology with one another and 88.3-96.5% with those reported for the isolate PV131 (BBWV-2). However, they have only 57.9-66.2% homology with those of PV132 and PV176 (BBWV-1).
Kobayashi
Y O
YO
National Agriculture Research Center, Tsukuba, Japan.
Nakano
M
M
Kashiwazaki
S
S
Naito
T
T
Mikoshiba
Y
Y
Shiota
A
A
Kameya-Iwaki
M
M
Honda
Y
Y
D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry; D019181:Fabavirus / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry*; D017385:Sequence Homology
D020413:3' Untranslated Regions; D012367:RNA, Viral