Binding of chemically-modified oligonucleotides to the double-stranded stem of an RNA hairpin.
We monitored the binding of triplex-forming oligopyrimidines to the double-stranded stem of the RNA hairpin responsible for the gag-pol frameshift in HIV-1. Whereas the substitution of 5, propynyl-C for C had a limited effect, the use of a Peptide Nucleic Acid 12mer led to a drastic reduction in the stability of the oligomer/RNA complex.
Aupeix
K
K
INSERM U 386, IFR Pathologies Infectieuses, Université Victor Segalen, Bordeaux, France.
Toulmé
J J
JJ
D001483:Base Sequence; D018965:Frameshifting, Ribosomal; D015685:Fusion Proteins, gag-pol / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry
D015685:Fusion Proteins, gag-pol; D009841:Oligonucleotides; D012330:RNA, Double-Stranded; D012367:RNA, Viral