Chemically distinct preganglionic inputs to iris-projecting postganglionic neurons in the rat: A light and electron microscopic study.
Individual autonomic postganglionic neurons are surrounded by pericellular baskets of preganglionic terminals that are easily identifiable with the light microscope. It has been assumed that the target cell of a pericellular basket of preganglionic terminals is the neuron at the centre of the basket. This assumption has enabled the connectivity of preganglionic neurons to be determined at the light microscopic level. However, if the preganglionic terminals in a pericellular basket make synapses with the dendrites of nearby, but functionally different, postganglionic neurons, then the conclusions of light microscopic studies are far less certain. We have used a serial section ultrastructural study to determine the target of the preganglionic pericellular basket in a situation where the apparent target cell is surrounded by neurons of dissimilar function. In the rat superior cervical ganglion, postganglionic neurons projecting to the iris were identified, using retrograde tracers, as single neurons (i.e., not in clusters). We have used immunohistochemistry to show that iris-projecting neurons are surrounded by preganglionic nerve terminals containing calcitonin gene-related peptide (CGRP). We have demonstrated that the pericellular basket of CGRP-immunoreactive preganglionic terminals provides inputs only to the soma at the centre of the basket and not to the dendrites of surrounding neurons. This suggests that, in autonomic ganglia, light microscopic identification of the preganglionic terminal baskets is likely to be a reliable method for identifying the targets of subclasses of preganglionic neurons.
Grkovic
I
I
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria 3052, Australia. i.grkovic@anatomy.unimelb.edu.au
Edwards
S L
SL
Murphy
S M
SM
Anderson
C R
CR
D000818:Animals; D001338:Autonomic Fibers, Postganglionic / Q000737:chemistry* / Q000166:cytology; D001339:Autonomic Fibers, Preganglionic / Q000737:chemistry* / Q000166:cytology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D005260:Female; D007498:Iris / Q000166:cytology*; D008297:Male; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000737:chemistry* / Q000166:cytology
D015740:Calcitonin Gene-Related Peptide