In vivo dominant lethal effect of pyrimethamine in male mouse germ cells.
Pyrimethamine is used for treatment of malaria and toxoplasmosis. The embryotoxicity and clastogenicity of pyrimethamine is known and our aim was to investigate its dominant lethal effect in vivo. For this purpose, we used three groups of Swiss-albino male mice and a control group. We injected males with doses of 16, 32 or 64 mg/kg pyrimethamine and housed them with 10 females/male for each mating interval. Females were sacrificed and their uteri were evaluated for dominant lethality. As a result of this study we found that pyrimethamine induced dominant lethal mutations in the third, fourth and sixth weeks at the 64 mg/kg dose level, without the effect being dose-dependent. We conclude that pyrimethamine is a suspected germ cell mutagen.
Egeli
U
U
Department of Medical Biology, Faculty of Medicine, Faculty of Science, University of Uludağ, Görükle/Bursa, Kocaeli, Turkey.
Aydemir
N
N
Akpinar
G
G
Cimen
C
C
Ergül
E
E
Tutar
G
G
Tunca
B
B
Bilaloğlu
R
R
D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005799:Genes, Dominant / Q000187:drug effects*; D005804:Genes, Lethal / Q000187:drug effects*; D005854:Germ Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics; D011247:Pregnancy; D011739:Pyrimethamine / Q000633:toxicity*; D013997:Time Factors
D011739:Pyrimethamine