[Preparation of McAb against lung cancer and its application in tumor radioimmunoimaging].
OBJECTIVE
In order to tracing the lung cancer, A monoclonal antibody was produced.


METHODS
The McAb, named SM-1, was specifically reacted with lung cancer confirmed by the immunopathological studies, then the McAb was digested into fragments by ficin and bromelain proteases.


RESULTS
Both of the enzymatic methods were very effective and the immuno-reactivity of fragments was retained well. In our localization experiments, the T/NT ratio revealed that radioiodinated McAb and fragments could be specifically uptaked by the tumour tissue and the T/NT ratio was higher in fragments group than intact IgG. After injection of 131I-IgG and fragments, the xenografts of human lung cancer bearing in nude mice were visualized distinctly during 36-48 hrs. Eighteen patients with lung cancer received Tc-99m labeled McAb intravenously, 13 cases showed radioactive accumulation in the foci of tumours.


CONCLUSIONS
These preliminary results suggested that SM-1 and its fragments might be a potential agents for radioimmunoimaging diagnosis of lung cancer.
Yang
Z
Z
PUMC Hospital, Beijing.
Wang
S
S
Sun
X
X
Li
F
F
Zhou
Q
Q
D000818:Animals; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging* / Q000473:pathology; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016719:Radioimmunodetection; D013667:Technetium; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007457:Iodine Radioisotopes; D013667:Technetium