title: An outbreak of Newcastle disease in free-living pheasants (Phasianus colchicus).
abstract: The epidemiology of an outbreak of Newcastle disease in a population of approximately 12,000 free-living pheasants (Phasianus colchicus) on the island of Faenø in Denmark in 1996 is described. The mortality during the epizootic was 56%. The spread of the disease between 7 groups of pheasants could be demonstrated over an observation period of 3 weeks. A total of 70 avian paramyxovirus serotype 1 (APMV-1) isolates was made from the flock. The intra cerebral pathogenicity indices of the 4 isolates tested were in the range 1.78-1.88. By means of immunoperoxidase monolayer assay with murine monoclonal antibodies and sequence analysis of an RT-PCR amplified segment of the F0 viral protein it was found, that the virus belonged to the highly virulent C1 antigenic group and that the amino acid sequence at the F0 cleavage site corresponded with the sequences of virulent APMV-1 strains. Based on the epidemiological circumstances it is believed that the virus was transmitted to the pheasants by feral birds.
authors.affiliation: Danish Veterinary Laboratory, Aarhus, Denmark.
authors.forename: P H
authors.initials: PH
authors.lastname: Jørgensen
authors.forename: K J
authors.initials: KJ
authors.lastname: Handberg
authors.forename: P
authors.initials: P
authors.lastname: Ahrens
authors.forename: H C
authors.initials: HC
authors.lastname: Hansen
authors.forename: R J
authors.initials: RJ
authors.lastname: Manvell
authors.forename: D J
authors.initials: DJ
authors.lastname: Alexander
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001717:Birds; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D003718:Denmark / Q000453:epidemiology; D004196:Disease Outbreaks / Q000662:veterinary*; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology*; D009522:Newcastle disease virus / Q000737:chemistry / Q000145:classification / Q000472:pathogenicity*; D013047:Specific Pathogen-Free Organisms; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000737:chemistry
chemical_list: D000914:Antibodies, Viral; D014760:Viral Fusion Proteins