title: Structure determination and refinement of the Al3+ complex of the D254,256E mutant of Arthrobacter D-xylose isomerase at 2.40 A resolution. Further evidence for inhibitor-induced metal ion movement.
abstract: The structure of the D254.256E double mutant of Arthrobacter xylose isomerase with Al3+ at both metal-binding sites was determined by the molecular replacement method at a conventional R-factor of 0.179. Binding of the two Al3+ does not alter the overall structure significantly. However, there are local rearrangements in the octahedral co-ordination sphere of the Al3+. The inhibitor molecule moves somewhat away from the active site. Furthermore, evidence was revealed for metal ion movement from site 2(1) to site 2(2) upon double mutation. Xylose isomerase requires two divalent metal cations for activation. The catalytic metal ion is translocated 1.8 A away from its initial position during the catalytic reaction. The fact that both activating and inactivating metals (including Al3+) were found exclusively at a single location in the double mutant was an indication that the consequently missing shuttle may account for the crippled catalytic efficiency.
authors.affiliation: Department of Theoretical Chemistry, L. Eötvös University, Budapest, Hungary.
authors.forename: T
authors.initials: T
authors.lastname: Gérczei
authors.forename: Z
authors.initials: Z
authors.lastname: Böcskei
authors.forename: E
authors.initials: E
authors.lastname: Szabó
authors.forename: B
authors.initials: B
authors.lastname: Asbóth
authors.forename: G
authors.initials: G
authors.lastname: Náray-Szabó
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000535:Aluminum / Q000378:metabolism*; D001173:Arthrobacter / Q000201:enzymology*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation
chemical_list: D004791:Enzyme Inhibitors; D000535:Aluminum; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D008274:Magnesium; D002118:Calcium