A 69 bp fragment in the pyrroline-5-carboxylate reductase promoter of Arabidopsis thaliana activates minimal CaMV 35S promoter in a tissue-specific manner.
The Arabidopsis thaliana gene that encodes pyrroline-5-carboxylate reductase (At-P5R), the last enzyme in proline biosynthesis in A. thaliana, is developmentally regulated and is highly expressed in cells that divide rapidly or undergo changes in osmotic potential. A 69 bp region (P69; -120 to -51) has previously been identified in a 5' deletion analysis of the At-P5R promoter to be necessary for the basal expression. Here, the essential role of P69 for tissue-specific expression of At-P5R is demonstrated by loss- and gain-of-function experiments.
Hua
X
X
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Belgium.
van de Cotte
B
B
Van Montagu
M
M
Verbruggen
N
N
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000502:physiology; D011762:Pyrroline Carboxylate Reductases / Q000235:genetics* / Q000502:physiology; D012335:RNA, Ribosomal / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D017384:Sequence Deletion
D010446:Peptide Fragments; D012335:RNA, Ribosomal; D011762:Pyrroline Carboxylate Reductases; D005966:Glucuronidase