Structural elucidation of the ceramide moiety of starfish gangliosides by collision-induced dissociation of the sodium ion complex.
Collision-induced dissociation (CID) spectra of sodium ion complexes ([M+Na]+ ions), produced by FAB-MS of methyl ester derivatives of ganglioside, indicate the length of the fatty acyl chain of the ceramide moieties without chemical degradation. In the case of a genuine ganglioside, only the fission of the glycosyl linkage of sialic acid was prominently observed.
Inagaki
M
M
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Isobe
R
R
Miyamoto
T
T
Higuchi
R
R
D000818:Animals; D002240:Carbohydrate Sequence; D002518:Ceramides / Q000737:chemistry*; D005732:Gangliosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012794:Sialic Acids / Q000737:chemistry; D012964:Sodium / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013215:Starfish / Q000737:chemistry*
D002518:Ceramides; D005732:Gangliosides; D012794:Sialic Acids; D012964:Sodium