title: Nuclear localization of a new c-cbl related protein, CARP 90, during in vivo thymic apoptosis in mice.
abstract: This study investigates the involvement of the c-cbl protooncogene in thymocyte apoptosis occurring in vivo after hydrocortisone treatment. In the thymus of untreated mice, a few medullary and cortical thymocytes expressed p120cbl, mainly in the cytoplasm. In the cortex, their number and distribution resemble that of apoptotic cells evidenced by TUNEL staining. The expression of Cbl is rapidly increased when apoptosis is triggered by hydrocortisone. This Cbl-specific immunostaining was detected in the nucleus and is due to a Cbl-related 90 kDa protein (CARP 90). These results show that a c-cbl product could localize in the nucleus and suggest that it could be involved as a regulator of thymic apoptosis.
authors.affiliation: Laboratory of Pathological Anatomy, CHU-B35, B-4000 Liège, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Denis
authors.forename: S
authors.initials: S
authors.lastname: Mandard
authors.forename: C
authors.initials: C
authors.lastname: Humblet
authors.forename: M
authors.initials: M
authors.lastname: Verlaet
authors.forename: J
authors.initials: J
authors.lastname: Boniver
authors.forename: D
authors.initials: D
authors.lastname: Stéhelin
authors.forename: M P
authors.initials: MP
authors.lastname: Defresne
authors.forename: D
authors.initials: D
authors.lastname: Régnier
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D050721:Proto-Oncogene Proteins c-cbl; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
chemical_list: D002843:Chromatin; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D050721:Proto-Oncogene Proteins c-cbl; D044767:Ubiquitin-Protein Ligases; C493849:Cbl protein, mouse; D006854:Hydrocortisone