Gene targeting restricted to mouse striated muscle lineage.
Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.
Miniou
P
P
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM, CNRS, ULP, BP163, 67404 Illkirch cedex, C.U. de Strasbourg, France.
Tiziano
D
D
Frugier
T
T
Roblot
N
N
Le Meur
M
M
Melki
J
J
D000199:Actins / Q000235:genetics; D000818:Animals; D002645:Chickens; D018390:Gene Targeting; D006801:Humans; D019426:Integrases / Q000235:genetics*; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014764:Viral Proteins
D000199:Actins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases