Granulocyte colony-stimulating factor and drugs elevating extracellular adenosine act additively to enhance the hemopoietic spleen colony formation in irradiated mice.
The effects of combined administration of two drugs elevating extracellular adenosine, namely dipyridamole (DP) and adenosine monophosphate (AMP), and granulocyte colony-stimulating factor (G-CSF) on hemopoietic stem cells in vivo were investigated. The experiments were performed on mice using the endogenous spleen colony formation in gamma-irradiated animals as an endpoint. The results have shown that DP and AMP act additively with G-CSF to enhance spleen colony formation and thus the erythroid repopulation of the spleen. These findings indicate that the signaling pathways of G-CSF and drugs elevating extracellular adenosine can interact at the level of primitive hemopoietic stem cells. The enhancement of hemopoiesis-stimulating effects of G-CSF by DP and AMP, which are low-priced and clinically available drugs, could improve the cost-effectiveness of the therapy with G-CSF.
Hofer
M
M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.
Pospísil
M
M
Netíková
J
J
Znojil
V
V
Vácha
J
J
D000241:Adenosine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002452:Cell Count; D003037:Cobalt Radioisotopes; D003114:Colony-Forming Units Assay; D004176:Dipyridamole / Q000494:pharmacology; D015672:Erythroid Precursor Cells / Q000166:cytology; D005110:Extracellular Space / Q000378:metabolism*; D005720:Gamma Rays; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology*; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes; D016179:Granulocyte Colony-Stimulating Factor; D000249:Adenosine Monophosphate; D004176:Dipyridamole; D000241:Adenosine