title: Temporal expression of the matrix metalloproteinase MMP-2 correlates with fibronectin immunoreactivity during the development of the vascular system in the chick embryo chorioallantoic membrane.
abstract: In this study we have examined in the chick embryo chorioallantoic membrane (ChAM) the expression of the matrix metalloproteinase 2 (MMP-2) and have correlated this parameter with the expansion of the ChAM vasculature and with the expression of 3 extracellular matrix components (fibronectin, laminin and type IV collagen), which differentially modulate angiogenesis. In the early phases of ChAM development, between d 6 and d 8 of incubation, when the increase of the ChAM vasculature is maximal, higher values of MMP-2 and, respectively, of fibronectin immunoreactive area, are detectable. These results indicate that MMP-2 activity and fibronectin expression are 2 strictly related components of angiogenesis occurring in vivo.
authors.affiliation: Institute of Human Anatomy, Histology and Embryology, University of Bari Medical School, Italy. ribatti@anatomia.uniba.it
authors.forename: D
authors.initials: D
authors.lastname: Ribatti
authors.forename: B
authors.initials: B
authors.lastname: Nico
authors.forename: A
authors.initials: A
authors.lastname: Vacca
authors.forename: M
authors.initials: M
authors.lastname: Iurlaro
authors.forename: L
authors.initials: L
authors.lastname: Roncali
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000378:metabolism*; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000098:blood supply* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D018093:Gelatinases / Q000032:analysis / Q000378:metabolism*; D005865:Gestational Age; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000032:analysis / Q000378:metabolism*; D018919:Neovascularization, Physiologic
chemical_list: D005353:Fibronectins; D007797:Laminin; D003094:Collagen; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2