Differential display analysis can reveal patterns of gene expression in immortalised hepatoma cells which are similar to those observed in young adult but not old adult liver cells.
We used the differential display technique to examine whether there were any patterns of gene expression which were characteristic of both young adult rat liver and of immortalised rat hepatoma cell lines, but not of old adult rat liver. No genes were detected which appeared to be clearly expressed in young liver and immortalised cell lines, but not in old liver. However, 14 genes were detected in old liver which were down-regulated in young liver and the hepatoma cell lines. This observation lends support to the idea that immortalisation of malignant cells may involve, at least in some aspects, a reversal of the ageing process in these cells and that the genes involved have a recessive action.
Charlton
M A
MA
Molecular Gerontology Unit, School of Sciences, University of Sunderland, UK.
Merry
B J
BJ
Goyns
M H
MH
D000375:Aging / Q000235:genetics; D000818:Animals; D002461:Cell Line, Transformed; D004247:DNA / Q000032:analysis*; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis*; D015870:Gene Expression; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004273:DNA, Neoplasm; D004247:DNA