[Angiogenesis in vitro].
A quantitative angiogenesis in vitro was investigated by culturing bovine carotid artery endothelial cells between two layers of type I collagen gel. Cells become organized into tube-like structures within few days. Ultrastructurally, tubular structures were composed of one to several endothelial cells containing pinocytotic vesicles and cytoplasmic projections, and linked by junctional complexes. A basal lamina-like structure surrounded the abluminal surface. Glucose, insulin, insulin-like growth factor I at pathophysiological high concentrations significantly stimulated tube-forming activity of endothelial cells by stimulating cell migration.
Hayashi
J
J
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital, Japan.
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D013268:Stimulation, Chemical
D007328:Insulin; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D005947:Glucose