Allo-immunization elicits CD8+ T cell-derived chemokines, HIV suppressor factors and resistance to HIV infection in women.
We assessed the potential for an allogeneic-based vaccine against HIV infection in women who were allo-immunized with their partners' mononuclear leucocytes to prevent spontaneous recurrent abortion. Within 1 month of allo-immunization, there was significant upregulation in the concentrations of CD8 cell-derived suppressor factor activity, RANTES, and macrophage inflammatory proteins 1alpha and 1beta. Allo-immunization also downregulated the proportion of cells with CCR5 and CXCR4 receptors. We also found a dose-dependent decrease in HIV infectivity of CD4+ cells in vitro after allo-immunization with both primary and T-cell line adapted HIV-1. This study provides a rational basis for an alternative or complementary strategy of allo-immunization against HIV infection.
Wang
Y
Y
Department of Immunobiology, Guy's, King's and St. Thomas' Medical and Dental Schools at Guy's Hospital, Medical School Building, 3rd Floor, London SE1 9RT, UK.
Tao
L
L
Mitchell
E
E
Bravery
C
C
Berlingieri
P
P
Armstrong
P
P
Vaughan
R
R
Underwood
J
J
Lehner
T
T
D000026:Abortion, Habitual / Q000276:immunology / Q000628:therapy; D015704:CD4 Antigens / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000378:metabolism; D019742:Chemokines, CC / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000637:transplantation; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D013491:Suppressor Factors, Immunologic / Q000276:immunology / Q000378:metabolism*; D014779:Virus Replication
D015704:CD4 Antigens; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019742:Chemokines, CC; D007518:Isoantibodies; D019402:Macrophage Inflammatory Proteins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D013491:Suppressor Factors, Immunologic