[Genetic mapping of the rice telomeric regions through PCR].
By using RAPD primer mediated asymmetric PCR (RM-PCR) method, a new type of molecular marker based on the telomeric repeats sequence was developed. PCR-based genetic mapping of telomeric repeat associated sequences (TASs) was conducted with a rice doubled haploid (DH) population derived from the inter-subspecific cross between indica variety (Zhaiyeqing8) and japonica variety (Jingxil7). Twenty-three loci were mapped onto the genetic map. Of these loci, 12 loci were mapped to the most distal position of eight chromosome arms and some of which may be located in subtelomeric region, five loci were mapped to the approximate positions of centromeric regions and six loci were mapped to other interstitial chromosomal regions.
Shen
L S
LS
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Zhu
L H
LH
D001483:Base Sequence; D002874:Chromosome Mapping; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D016615:Telomere