Antitumoral effects of squamocin on parental and multidrug resistant MCF7 (human breast adenocarcinoma) cell lines.
The antiproliferative effects of squamocin, one of the easiest annonaceous acetogenins to obtain, were studied in the parental (MCF7-S) and the multidrug resistant (MCF7-R) human breast adenocarcinoma cell lines. Squamocin inhibited proliferation of both cell lines identically, by blocking the cell cycle in the G1-phase. This inhibition was reversible in the long term. Squamocin decreased the ATP pool in both MCF7 cell lines, but did not seem to induce apoptosis. Cytotoxic activity of adriamycin was not restored in MCF7-R Pgp expressing cells by squamocin addition.
Raynaud
S
S
Laboratoire de Pharmacognosie, URA CNRS 1843 (BIOCIS), Faculté de Pharmacie, Université de Paris XI, Châtenay-Malabry, France. reynald.hocquemiller@cep.u-psud.fr
Némati
F
F
Miccoli
L
L
Michel
P
P
Poupon
M F
MF
Fourneau
C
C
Laurens
A
A
Hocquemiller
R
R
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use; D018432:Drug Resistance, Multiple; D005260:Female; D005663:Furans / Q000494:pharmacology* / Q000627:therapeutic use; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D005663:Furans; D007783:Lactones; C059660:squamocin; D004317:Doxorubicin