Isotype-specific changes in the amount of beta-tubulin RNA in synchronized tobacco BY2 cells.
The 3'-ends of the beta-tubulin cDNA were amplified from tobacco BY2 polyA+ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which "EEGDYYEEDEEDLNEA", NTB2 in which "EEEYYEDEEEA QED" and NTB3 in which "DECEYEEEEEYDHEGN" follows the conservative "YQQYQDATAD" sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each beta-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2.
Okamura
S
S
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan. okamura@ms.toyama-mpu.ac.jp
Okahara
K
K
Iida
T
T
Ozaki
M
M
Asano
S
S
Morita
M
M
Imanaka
T
T
D020413:3' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D015151:Immunoblotting; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D014404:Tubulin; D012313:RNA