title: [Bacterial keratitis. Microbiological analysis as a principle for therapeutic recommendations].
abstract: OBJECTIVE
  The study presented differentiates between the aetiological agents of bacterial keratitis in patients with and without a history of contact lens wear. Based on these results, recommendations are given for optimal antibiotic primary therapy.
  
  
  METHODS
  Swabs and corneal scrapings were taken from 218 patients referred to the University Eye Hospital in Munich with a diagnosis of bacterial keratitis from 1989 to 1997. Ninety-two of these patients had a history of contact lens wear; 126 had none. The germs were isolated and identified by staining and microscopy. Observing polymicrobial growth in 51 patients, a total of 275 germs was isolated.
  
  
  RESULTS
  The most frequent pathogens were Staphylococcus epidermidis (44%), S. aureus (18%), Streptococcus spp. (10%), Propionibacterium acnes (7%) and Pseudomonas aeruginosa (6%). Gram-negative germs were nearly exclusively isolated from contact lens wearers, gram-positive germs were predominant in non-contact lens wearers.
  
  
  CONCLUSIONS
  Keratitis in patients with a history of contact lens wear is often caused by aggressive gram-negative germs. Those cases should immediately be treated with quinolones and erythromycin. In keratitis caused by gram-positive pathogens, a combination with aminoglycosides and erythromycin is sufficient.
authors.affiliation: Augenklinik der Ludwig-Maximilians-Universität München.
authors.forename: S J
authors.initials: SJ
authors.lastname: Fröhlich
authors.forename: H
authors.initials: H
authors.lastname: Miño de Kaspar
authors.forename: T
authors.initials: T
authors.lastname: Grasbon
authors.forename: C
authors.initials: C
authors.lastname: Möhring
authors.forename: V
authors.initials: V
authors.lastname: Klauss
authors.forename: A
authors.initials: A
authors.lastname: Kampik
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001419:Bacteria / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D003261:Contact Lenses; D004352:Drug Resistance, Microbial; D005260:Female; D006801:Humans; D007223:Infant; D007634:Keratitis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged
chemical_list: D000900:Anti-Bacterial Agents