Isolation, characterization, and chromosomal localization of the human ENSA gene that encodes alpha-endosulfine, a regulator of beta-cell K(ATP) channels.
Human alpha-endosulfine is an endogenous regulator of the beta-cell K(ATP) channels. The recombinant alpha-endosulfine inhibits sulfonylurea binding to beta-cell membranes, reduces cloned K(ATP) channel currents, and stimulates insulin secretion from beta-cells. These properties led us to study the human ENSA gene that encodes alpha-endosulfine. Here, we describe the isolation, the partial characterization, and the chromosomal localization of the ENSA gene. The ENSA gene appears to be a 1.8-kb-long sequence that contains the transcription initiation site located 528 bp upstream of the initiation codon. The ENSA gene is intronless, and a single copy gene seems to be present in the genome. Finally, the ENSA gene co-localizes on human chromosome 14 (14q24.3-q31) with a locus for susceptibility to type 1 diabetes called IDDM11; thus, the ENSA gene represents an IDDM11 candidate.
Héron
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Institut National de la Santé et de la Recherche Médicale U376, CHU Arnaud-de-Villeneuve, Montpellier, France.
Virsolvy
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A
Apiou
F
F
Le Cam
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A
Bataille
D
D
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D005815:Genetic Code; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007515:Islets of Langerhans / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism
D029721:Drosophila Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D015221:Potassium Channels; C483180:endos protein, Drosophila; C075663:endosulfine; D000255:Adenosine Triphosphate