Chromosomal aberrations in human hepatocellular carcinomas associated with hepatitis C virus infection detected by comparative genomic hybridization.
Thirty-five hepatocellular carcinomas (HCCs) associated with hepatitis C virus (HCV) were analysed by comparative genomic hybridization (CGH), to screen for changes in copy-number of DNA sequences. Chromosomal losses were noted in 1p34-36 (37%), 4q12-21 (48%), 5q13-21 (35%), 6q13-16 (23%), 8p21-23 (28%), 13q (20%), 16q (33%) and 17p13 (37%). Gains were noted in 1q (46%), 6p (20%), 8q21-24 (31%) and 17q (43%). High level gains indicative of gene amplifications were found in 7q31 (3%), 11q13 (3%), 14q12 (6%) and 17q12 (3%); amplification at 14q12 may be characteristic for HCCs. No significant difference in chromosomal aberrations was noted between carcinomas associated with HCV-infection in our study and those reported earlier in HCCs infected with hepatitis B virus (HBV), indicating that both HBV- and HCV-related carcinomas may progress through a similar cascade of molecular events.
Sakakura
C
C
First Department of Surgery, Kyoto Prefectural University of Medicine, Kawaramachi-dori, Japan.
Hagiwara
A
A
Taniguchi
H
H
Yamaguchi
T
T
Yamagishi
H
H
Takahashi
T
T
Koyama
K
K
Nakamura
Y
Y
Abe
T
T
Inazawa
J
J
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D005260:Female; D006526:Hepatitis C / Q000150:complications / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D019656:Loss of Heterozygosity; D008297:Male; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D012016:Reference Values