Application of high energy shock waves to cancer treatment in combination with cisplatin and ATX-70.
BACKGROUND
The anti-tumor effects of high-energy shock waves (HESW) in combination with cisplatin (CDDP) and ATX-70, as a photosensitizer, were investigated in vitro and in vivo.


METHODS
Active oxygen generation by HESW was studied by means of electron spin resonance (ESR) system. The anti-tumor effects of HESW in combination with cisplatin (CDDP) and ATX-70 was investigated in vitro and in vivo.


RESULTS
HESW exposure induced singlet oxygen or hydroxyl radical. Adding ATX-70 enhanced the cell damage induced by HESW dose-dependently. Ascorbic acid inhibited the combined effect of HESW and ATX-70. Adding CDDP did not enhanced cell damage by HESW. The combined therapy of HESW, CDDP and ATX-70 showed further inhibition of tumor growth in vivo.


CONCLUSIONS
These results indicate that HESW activated ATX-70, and that the anti-tumor effect of HESW and ATX-70 was caused by active oxygen generation. HESW is able to be utilized with any other cancer treatment.
Maruyama
M
M
Second Department of Surgery, Chiba University School of Medicine, Japan.
Asano
T
T
Nakagohri
T
T
Uematsu
T
T
Hasegawa
M
M
Miyauchi
H
H
Iwashita
C
C
Isono
K
K
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002945:Cisplatin / Q000008:administration & dosage; D003131:Combined Modality Therapy; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D019220:High-Energy Shock Waves / Q000627:therapeutic use*; D017665:Hydroxyl Radical / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000628:therapy*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D018384:Oxidative Stress; D010100:Oxygen / Q000032:analysis; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000494:pharmacology; D011166:Porphyrins / Q000008:administration & dosage / Q000494:pharmacology; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; D014407:Tumor Cells, Cultured / Q000637:transplantation
D000975:Antioxidants; D005609:Free Radicals; D017319:Photosensitizing Agents; D011166:Porphyrins; D017382:Reactive Oxygen Species; C081908:ATX 70; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D001205:Ascorbic Acid; D002945:Cisplatin; D010100:Oxygen