title: Regulation of the miniature plasma membrane Ca(2+) channel I(min) by inositol 1,4,5-trisphosphate receptors.
abstract: I(min) is a plasma membrane-located, Ca(2+)-selective channel that is activated by store depletion and regulated by inositol 1,4, 5-trisphosphate (IP(3)). In the present work we examined the coupling between I(min) and IP(3) receptors in excised plasma membrane patches from A431 cells. I(min) was recorded in cell-attached mode and the patches were excised into medium containing IP(3). In about 50% of experiments excision caused the loss of activation of I(min) by IP(3.) In the remaining patches activation of I(min) by IP(3) was lost upon extensive washes of the patch surface. The ability of IP(3) to activate I(min) was restored by treating the patches with rat cerebellar microsomes reach in IP(3) receptors but not by control forebrain microsomes. The re-activated I(min) had the same kinetic properties as I(min) when it is activated by Ca(2+)-mobilizing agonists in intact cells and by IP(3) in excised plasma membrane patches and it was inhibited by the I(crac) inhibitor SKF95365. We propose that I(min) is a form of I(crac) and is gated by IP(3) receptors.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia 194064, USA.
authors.forename: A I
authors.initials: AI
authors.lastname: Zubov
authors.forename: E V
authors.initials: EV
authors.lastname: Kaznacheeva
authors.forename: A V
authors.initials: AV
authors.lastname: Nikolaev
authors.forename: V A
authors.initials: VA
authors.lastname: Alexeenko
authors.forename: K
authors.initials: K
authors.lastname: Kiselyov
authors.forename: S
authors.initials: S
authors.lastname: Muallem
authors.forename: G N
authors.initials: GN
authors.lastname: Mozhayeva
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015640:Ion Channel Gating; D007700:Kinetics; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism* / Q000502:physiology; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D015220:Calcium Channels; C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008565:Membrane Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate