Immunocytochemical mapping of the hemoglobin biosynthesis site in amphibian erythroid cells.
During the past 25 years, several studies have attempted to determine the site of integration of the heme and the four globin chains in vertebrate erythroid cells that is important in the formation of the hemoglobin molecule. Mitochondrion-like organelles or hemosomes were pointed out as responsible for this task. We performed several experiments to investigate this hypothesis. The intracellular distribution of hemoglobin in amphibian erythroid cells was detected by post-embedding immuno-electron microscopy, using a polyclonal anti-human hemoglobin-proteinA-gold complex. Hemoglobin mapping showed an intense labeling in the cell cytoplasm, but none in cytoplasmic structures such as endoplasmic reticulum, mitochondria, mitochondrion-like organelles, Golgi complex, ribosomes or ferruginous inclusions. The mitochondrial fraction obtained according to the protocol described for some authors, showed by ultrastructural examination that this fraction has a heterogeneous content, also composed by microvesicles rich in cytoplasmic hemoglobin, an artifact generated by mechanical action during cell fractionation. Thus, when this fraction is lysed and its content submitted to electrophoresis, hemoglobin bands would be found inevitably, causing false-positive results, erroneously attributed to hemoglobin content of mitochondrion-like organelles. Our data do not confirm the hypothesis that the final hemoglobin biosynthesis occurs inside mitochondrion-like organelles. They suggest that the hemoglobin molecule be assembled in the erythrocyte cytoplasm outside of mitochondria or hemosomes.
Cianciarullo
A M
AM
Laboratório de Genética, Instituto Butantan, São Paulo, Brazil. amcianciarullo@hotmail.com
Beçak
W
W
Soares
M J
MJ
D000818:Animals; D002025:Bufonidae; D004900:Erythroblasts / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006454:Hemoglobins / Q000096:biosynthesis*; D007150:Immunohistochemistry; D017348:Microscopy, Electron, Scanning Transmission; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D011898:Ranidae
D006454:Hemoglobins