Proliferation and immunoglobulin secretion of lymphoblastoid cell lines are differently affected by soluble cytokines.
The aim of our study was to investigate whether supernatant from lipopolysaccharide-activated monocytes (monocyte-factor) and/or cytokines could enhance secretion of human monoclonal antibodies specific to HLA antigens produced by Epstein-Barr virus lymphoblastoid cell lines (EBV-LCLs). In a low cell density culture system, the monocyte-factor significantly stimulated cell growth of three monoclonal and two polyclonal EBV-LCLs while no enhancement of immunoglobulin production was observed. The enhancement of proliferation was completely neutralized by an antiserum to human IL-6 suggesting that IL-6 was required for the stimulation of growth of LCLs. The effect of cytokines on proliferation showed large variations among the cell lines, with IL-1beta generally inducing the highest response. Of the cytokines tested, only IL-2 was able to enhance total immunoglobulin secretion due to the induction of a higher production of light chains. The specific anti-HLA activity was slightly increased by IL-10 although this cytokine had no effect on total immunoglobulin concentration or proliferation.
Pistillo
M P
MP
National Institute for Cancer Research, Genova, Italy.
Cella
M
M
Mantero
S
S
Ferrara
G B
GB
D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D016922:Cellular Senescence / Q000502:physiology; D016207:Cytokines / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007136:Immunoglobulins / Q000378:metabolism*; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D012995:Solubility
D016207:Cytokines; D007106:Immune Sera; D007136:Immunoglobulins; D015850:Interleukin-6; D008070:Lipopolysaccharides