Experimental infection of canine peritoneal macrophages with visceral and dermotropic Leishmania strains.
A study was carried out using macrophages cultured from the peritoneal exudate of dogs infected in vitro with three species of Leishmania: L. (L.) chagasi, L. (Viannia) braziliensis and L. (L.) amazonensis with the aim of investigating the growth kinetics and infectivity of these species in the host cell. Results were expressed as the percentage of macrophages infected measured at 24 hr intervals over six days in RPMI - 1640 culture medium at a temperature of 34-35 degrees C. The findings open the possibility of using canine peritoneal cells as a model for the screening of leishmanicide drugs and to study the pathogenesis of these species.
Madeira
M
M
Departamento de Ciências Biológicas, Escola Nacional de Saúde Pública, Fiocruz, Rio de Janeiro, RJ, 21041-210, Brasil. fmadeira@ensp.fiocruz.br
Barbosa-Santos
E
E
Marzochi
M
M
D000818:Animals; D004285:Dogs; D005260:Female; D007891:Leishmania / Q000472:pathogenicity*; D007892:Leishmania braziliensis / Q000472:pathogenicity; D018314:Leishmania infantum / Q000472:pathogenicity; D007894:Leishmania mexicana; D016773:Leishmaniasis, Cutaneous; D007897:Leishmaniasis, Mucocutaneous; D007898:Leishmaniasis, Visceral; D017737:Macrophages, Peritoneal / Q000469:parasitology*; D008297:Male