Increased frequency of recognition of delipidated versus intact CNS myelin in multiple sclerosis and control subjects.
The proliferative response of mononuclear cells from MS patients and normal control subjects to intact and delipidated myelin membranes was examined. The mean frequency of recognition in both groups of human subjects was greater for delipidated myelin than for intact myelin. Human T cell lines established using intact or delipidated myelin as the antigen were highly heterogeneous in response, and were each able to recognize myelin basic protein and myelin proteolipid protein peptides. However, there was no difference in the frequency of recognition of either form of myelin membrane when MS patients were compared to control subjects. Our results suggest that the presentation of delipidated forms of membrane proteins might enhance the response to myelin antigens in vivo, and be relevant to demyelinating diseases.
Trotter
J L
JL
Department of Neurology and Neurosurgery, Washington University School of Medicine, St. Louis, MO 63110, USA. trotterj@neuro.wustl.edu
Keadle
T L
TL
Lauber
J M
JM
Dong
W L
WL
Cross
A H
AH
D000328:Adult; D016022:Case-Control Studies; D002455:Cell Division / Q000502:physiology; D002490:Central Nervous System / Q000378:metabolism*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology
D008563:Membrane Lipids; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein