Immunolocalization of cyclooxygenase-2 in the macula densa of human elderly.
To gain insight into the role of prostanoids in human kidney function, we examined the distribution of cyclooxygenase (COX) 1 and COX-2 by immunofluorescence and immunohistochemistry in human kidneys from adults of various age groups. COX-1 was detected in the collecting ducts, thin loops of Henle and portions of the renal vasculature. COX-2 was detected in the renal vasculature, medullary interstitial cells, and the macula densa. In addition, COX-2 immunoreactivity was noted in afferent arteries and the macula densa of the renal cortex and was more evident in the kidneys of older adults.
Nantel
F
F
Department of Biochemistry and Molecular Biology, Merck Frosst Center for Therapeutic Research, P.O. Box 1005, Dorval-Pointe-Claire, Que., Canada. francois_nantel@merck.com
Meadows
E
E
Denis
D
D
Connolly
B
B
Metters
K M
KM
Giaid
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged / Q000502:physiology*; D000369:Aged, 80 and over; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000166:cytology* / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000276:immunology / Q000378:metabolism*; D012016:Reference Values; D013194:Staining and Labeling / Q000379:methods
D007527:Isoenzymes; D008565:Membrane Proteins; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases