title: Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.
abstract: The conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >/=195-fold higher (amoxicillin).
authors.affiliation: Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, United Kingdom.
authors.forename: N A
authors.initials: NA
authors.lastname: Cross
authors.forename: D J
authors.initials: DJ
authors.lastname: Kellock
authors.forename: G R
authors.initials: GR
authors.lastname: Kinghorn
authors.forename: M
authors.initials: M
authors.lastname: Taraktchoglou
authors.forename: E
authors.initials: E
authors.lastname: Bataki
authors.forename: K M
authors.initials: KM
authors.lastname: Oxley
authors.forename: P M
authors.initials: PM
authors.lastname: Hawkey
authors.forename: A
authors.initials: A
authors.lastname: Eley
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002692:Chlamydia trachomatis / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D004917:Erythromycin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D004917:Erythromycin; D000658:Amoxicillin