Molecular cloning of the Pasteurella haemolytica pomA gene and identification of bovine antibodies against PomA surface domains.
The gene (pomA) encoding PomA, an OmpA-like major outer membrane protein of the bovine respiratory pathogen Pasteurella haemolytica, was cloned, and its nucleotide sequence was determined. The deduced amino acid sequence of PomA has significant identity with the sequences of other OmpA family proteins. Absorption of three different bovine immune sera with whole P. haemolytica cells resulted in a reduction of bovine immunoglobulin G reactivity with recombinant PomA in Western immunoblots, suggesting the presence of antibodies against PomA surface domains.
Zeng
H
H
Department of Anatomy, Pathology, and Pharmacology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
Pandher
K
K
Murphy
G L
GL
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D005798:Genes, Bacterial; D016978:Mannheimia haemolytica / Q000276:immunology*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D018985:Epitopes, B-Lymphocyte; C103352:PomA protein, bacteria; D011993:Recombinant Fusion Proteins