Regulation of complement factor H expression in L cells.
The luciferase system was used to assay basal promoter activity of the murine factor H gene in the fibroblast cell line L929 (L cells). Thirteen nested deletion constructs were tested, and a region between -811 and -344 was found to have enhancer activity in the context of a heterologous promoter. This fragment was subdivided further and each of the two resulting subfragments also had enhancer activity. These subfragments each were shifted in electrophoretic mobility shift assays and were able to cross-inhibit each other in binding to a nuclear factor. Sequence analysis of these subfragments revealed the presence of an octamer in each subfragment, and a synthetic oligomer containing this octamer sequence was able to block binding in the mobility shift assay. Thus, this octamer sequence appears to play a major role in the basal expression of the factor H gene in L cells.
Vik
D P
DP
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico, 87131-5276, USA. dvik@unm.edu
D000818:Animals; D002460:Cell Line; D017242:Complement Factor H / Q000096:biosynthesis / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion
D009687:Nuclear Proteins; D011994:Recombinant Proteins; D017242:Complement Factor H; D008156:Luciferases