title: A reverse-sandwich enzyme-linked immunosorbent assay for verocytotoxin 1 and 2 antibodies in human and bovine sera.
abstract: A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and animal sera. This assay has two advantages over a conventional indirect ELISA: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. The VT1 (Shiga-like toxin 1) antibody-positive rates were 5% in 202 normal adult humans and 99% in 93 normal cattle at a dairy farm. This ELISA is most suitable for seroepidemiologic studies of infections with VT-producing Escherichia coli in humans and various animal species.
authors.affiliation: Department of Medical Technology, Kyorin University School of Health Sciences, Hachioji-shi, Japan. hiro_mzw@d1.dion.ne.jp
authors.forename: H
authors.initials: H
authors.lastname: Miyazawa
authors.forename: H
authors.initials: H
authors.lastname: Bannai
authors.forename: T
authors.initials: T
authors.lastname: Yanase
authors.forename: C
authors.initials: C
authors.lastname: Morita
authors.forename: S
authors.initials: S
authors.lastname: Satoh
authors.forename: J
authors.initials: J
authors.lastname: Sugiyama
authors.forename: S
authors.initials: S
authors.lastname: Taniguchi
authors.forename: S
authors.initials: S
authors.lastname: Inouye
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000918:Antibody Specificity; D001427:Bacterial Toxins / Q000032:analysis* / Q000097:blood / Q000276:immunology; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003612:Dairying; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D011817:Rabbits; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D017186:Titrimetry; D015047:Zoonoses
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2