Autohydrolysis of phytic acid.
The autohydrolysis of phytic acid at 120 degrees C resulted in the formation of most of the phosphate esters of myo-inositol in varying amounts depending upon the reaction time. Eighteen of the 39 chromatographically distinct myo-inositol mono-, bis-, tris-, tetrakis-, pentakis-, and hexakisphosphates have been characterized using two different HPLC systems. These myo-inositol phosphates were partially purified by preparative anion-exchange chromatography under acidic and alkaline elution conditions. The combination of these two methods provides a two-tiered chromatographic approach to the rapid and sensitive identification of inositol phosphates in complex mixtures. Identification of the products was confirmed by 1D and 2D (1)H NMR analysis. The analytical procedure was applied to the autohydrolysis of the mixture of inositol phosphates from corn steep water.
Hull
S R
SR
Department of Pathology, Department of Biochemistry, University of Iowa, Iowa, Iowa City 52242, USA.
Gray
J S
JS
Montgomery
R
R
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006358:Hot Temperature; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D010833:Phytic Acid / Q000737:chemistry*
D007295:Inositol Phosphates; D010833:Phytic Acid