title: Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains.
abstract: Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28 degree C in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ss-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and xylopentaose. However, the enzyme was not able to degrade xylohexaose. Xylanase pretreatment was effective for hardwood kraft pulp bleaching. Hardwood kraft pulp bleached in the XEOP sequence had its kappa number reduced from 13.2 to 8.9 and a viscosity of 20. 45 cp. The efficiency of delignification was 33%.
authors.affiliation: Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brasil.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Silveira
authors.forename: F A
authors.initials: FA
authors.lastname: Ximenes
authors.forename: A O
authors.initials: AO
authors.lastname: Cacais
authors.forename: A M
authors.initials: AM
authors.lastname: Milagres
authors.forename: C L
authors.initials: CL
authors.lastname: Medeiros
authors.forename: J
authors.initials: J
authors.lastname: Puls
authors.forename: E X
authors.initials: EX
authors.lastname: Filho
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D014242:Trichoderma / Q000201:enzymology*; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D014990:Xylans; D014995:Xylosidases