Dynamic regulation of cpg15 during activity-dependent synaptic development in the mammalian visual system.
During visual system development, neural activity regulates structural changes in connectivity including axonal branching and dendritic growth. Here we have examined a role for the candidate plasticity gene 15 (cpg15), which encodes an activity-regulated molecule that can promote dendritic growth, in this process. We report that cpg15 is expressed in the cat visual system at relatively high levels in the lateral geniculate nucleus (LGN) but at very low levels in its synaptic target, layer 4 of the visual cortex. Prenatally, when cpg15 mRNA in the LGN is most abundant, expression is insensitive to action potential blockade by tetrodotoxin. Postnatally, activity regulation of cpg15 emerges in the LGN coincident with development of ocular dominance columns in the visual cortex. cpg15 can be detected in layers 2/3 and 5/6 of visual cortex postnatally, and expression in layers 2/3 is activity-regulated during known periods of activity-dependent plasticity for these layers. Localization and regulation of cpg15 expression in the visual system are consistent with a presynaptic role for CPG15 in shaping dendritic arbors of target neurons during activity-dependent synaptic rearrangements, both in development and adulthood.
Corriveau
R A
RA
Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
Shatz
C J
CJ
Nedivi
E
E
D000200:Action Potentials / Q000187:drug effects; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage / Q000494:pharmacology; D002415:Cats; D003001:Cloning, Molecular; D003712:Dendrites / Q000502:physiology; D005314:Embryonic and Fetal Development; D007839:Functional Laterality; D018507:Gene Expression Regulation, Developmental; D005829:Geniculate Bodies / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D007276:Injections, Intraventricular; D008565:Membrane Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D013779:Tetrodotoxin / Q000008:administration & dosage / Q000494:pharmacology; D014158:Transcription, Genetic; D015349:Vision, Monocular / Q000502:physiology; D014793:Visual Cortex / Q000196:embryology / Q000254:growth & development / Q000502:physiology*
D019208:Brain-Derived Neurotrophic Factor; C114622:Cpg15 protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D013779:Tetrodotoxin