Loss of matrix-dependent cytoskeletal tyrosine kinase signals may regulate intestinal epithelial differentiation during mucosal healing.
Intestinal epithelial restitution and the migratory phenotype appear regulated by the extracellular matrix. Since integrin-associated adhesion to matrix triggers tyrosine kinase activity, we hypothesized that matrix-specific tyrosine kinase signals might modulate the intestinal epithelial migratory phenotype, particularly via focal adhesion kinase. Caco-2 cells were seeded at two densities on collagen I, laminin, fibronectin, and tissue culture plastic. Four days later the first cells were confluent, whereas the second cells were not contact inhibited and expressed migratory lamellipodia. Cells were fractionated into membrane/cytoskeletal and cytosolic fractions. Cytoskeletal tyrosine kinase activity in static cells was matrix dependent and, unlike cytoscolic tyrosine kinase, correlated with adhesion, highest on collagen and lowest on plastic. Migrating cells exhibited matrix-dependent increases in cystosolic tyrosine kinase activity. Cytosolic changes in tyrosine kinase activity in motile cells exceeded membrane/cytoskeletal changes. However, matrix-dependent variations in increase in cytosolic tyrosine kinase activity correlated inversely with changes in cytoskeletal tyrosine kinase activity, suggesting cytoskeletal tyrosine kinase translocation to the cytosol during motility. Indeed cytoskeletal focal adhesion kinase activity decreased during migration on collagen. Tyrosine kinase inhibition by genistein both inhibited migration and stimulated expression of brush-border enzymes downregulated during motility. Although enterocyte-matrix interactions alter both cytosolic and cytoskeletal tyrosine kinase activity, matrix-dependent cytoskeletal events are likely to regulate adhesion and differentiation in static cells. Loss of matrix-dependent cytoskeletal tyrosine kinase signals such as focal adhesion kinase during restitution may trigger a phenotypic switch to the "dedifferentiated" migrating intestinal epithelial phenotype.
Liu
Y W
YW
Departments of Surgery, Yale University and Connecticut VA Health Care System, New Haven, Connecticut 06520-8062, USA.
Sanders
M A
MA
Basson
M D
MD
D018938:Caco-2 Cells; D002454:Cell Differentiation; D002465:Cell Movement; D003599:Cytoskeleton / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000201:enzymology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D014945:Wound Healing / Q000502:physiology*
D011505:Protein-Tyrosine Kinases