title: Ambient pH signal transduction in Aspergillus: completion of gene characterization.
abstract: Completing the molecular analysis of the six pal genes of the ambient pH signal transduction pathway in Aspergillus nidulans, we report the characterization of palC and palH. The derived translation product of palH contains 760 amino acids with prediction of seven transmembrane domains in its N-terminal moiety. Remarkably, a palH frameshift mutant lacking just over half the PalH protein, including almost all of the long hydrophilic region C-terminal to the transmembrane domains, retains some PalH function. The palC-derived translation product contains 507 amino acids, and the null phenotype of a frameshift mutation indicates that at least one of the C-terminal 142 residues is essential for function. Uniquely among the A. nidulans pH-signalling pal genes, palC appears to have no Saccharomyces cerevisiae homologue, although it does have a Neurospora crassa expressed sequence tag homologue. In agreement with findings for the palA, palB and palI genes of this signalling pathway, levels of the palC and palH mRNAs do not appear to be pH regulated.
authors.affiliation: Department of Infectious Diseases, Imperial College School of Medicine at Hammersmith Hospital, Du Cane Road, London W12 ONN, UK.
authors.forename: S
authors.initials: S
authors.lastname: Negrete-Urtasun
authors.forename: W
authors.initials: W
authors.lastname: Reiter
authors.forename: E
authors.initials: E
authors.lastname: Diez
authors.forename: S H
authors.initials: SH
authors.lastname: Denison
authors.forename: J
authors.initials: J
authors.lastname: Tilburn
authors.forename: E A
authors.initials: EA
authors.lastname: Espeso
authors.forename: M A
authors.initials: MA
authors.lastname: Peñalva
authors.forename: H N
authors.initials: HN
authors.lastname: Arst
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D016368:Frameshift Mutation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; C121839:PALC protein, Emericella nidulans; C121840:PALH protein, Emericella nidulans