Expression of gastrin/CCK-B receptors does not lead to a mitogenic response to gastrin in two colon cell lines.
To clarify the impact of the classic gastrin/CCK-B receptor on the growth of benign and malignant colonic cells, two permanent cell lines expressing this receptor have been established. The conditionally immortalized nonmalignant colonic cell line YAMC and the colonic carcinoma cell line SW 403 were stably transfected with a plasmid encoding the gastrin/CCK-B receptor (GR), or with plasmid alone (V). Expression of the gastrin/CCK-B receptor in the transfected YAMC-GR and SW 403-GR cells was demonstrated by gastrin binding experiments. The YAMC-GR cell line did not respond mitogenically to pentagastrin or gastrin(17) in vitro and was not tumorigenic. The SW 403-GR cell line was stimulated by gastrin(17) in vitro, but the growth patterns of SW 403-GR and SW 403-V were the same in nude mice with cells injected either subcutaneously or into the spleen. These results provide further evidence that the gastrin/CCK-B receptor is not responsible for gastrin-stimulated growth in colonic tumors.
Imdahl
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Chirurgische Universitätsklinik, Hugstettertrasse 55, Freibrug, D-79106, Germany. Imdahl@ch11.ukl.uni-freiburg.de
Hock
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M
Kuzeawu
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Mantamadiotis
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T
Baldwin
G S
GS
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D003106:Colon / Q000166:cytology / Q000187:drug effects* / Q000196:embryology*; D005755:Gastrins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008934:Mitogens / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D010418:Pentagastrin / Q000494:pharmacology; D011949:Receptors, Cholecystokinin / Q000378:metabolism*; D012878:Skin Neoplasms / Q000473:pathology; D013160:Splenic Neoplasms / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000473:pathology
D005755:Gastrins; D008934:Mitogens; D011949:Receptors, Cholecystokinin; C013550:gastrin 17; D010418:Pentagastrin