Effects of culture parameters on the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenol (2,4-DCP) by selected fungi. Groupe pour l'Etude du Devenir des Xénobiotiques dans l'Environnement (GEDEXE).
In order to enhance 2,4-D and 2,4-DCP degradation by four selected fungi (Cunninghamella elegans, C. echinulata, Rhizoctonia solani and Verticillium lecanii), three culture parameters (initial chemical concentration, amounts of glucose and nitrogen) were varied. The levels of both xenobiotics in the culture media were monitored by HPLC analysis after five days of cultivation. The best results were obtained at low initial concentration (20 mg.L-1 vs 100) and with low amounts of glucose (5 g.L-1 vs 10) and nitrogen (2.4 mM vs 24). When these two elements were lacking from the culture media, biodegradation was not suppressed, but took place to a lesser extent. Thus, initial chemical concentration and amounts of carbon and nitrogen, in the culture medium, were shown to strongly influence the extent of 2,4-D and 2,4-DCP removal by fungi.
Vroumsia
T
T
Laboratoire de Botanique, Cryptogamie, Biologie Cellulaire et Génétique, UFR de Pharmacie de Grenoble, Université J. Fourier, Meylan, France.
Steiman
R
R
Seigle-Murandi
F
F
Benoit-Guyod
J L
JL
D015084:2,4-Dichlorophenoxyacetic Acid / Q000493:pharmacokinetics*; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000493:pharmacokinetics*; D003470:Culture Media; D005658:Fungi / Q000378:metabolism; D006540:Herbicides / Q000493:pharmacokinetics*; D012989:Soil Pollutants / Q000493:pharmacokinetics*; D015262:Xenobiotics / Q000493:pharmacokinetics
D002733:Chlorophenols; D003470:Culture Media; D006540:Herbicides; D012989:Soil Pollutants; D015262:Xenobiotics; D015084:2,4-Dichlorophenoxyacetic Acid; C004762:2,4-dichlorophenol