title: Modulatory effect of divalent metal cations on the phosphotyrosine activity of the frog liver acid phosphatase.
abstract: Frog liver acid phosphatase hydrolyzes phosphotyrosine at acidic pH optimum. Mn2+, Ca2+ and Mg2+ (but not Zn2+) ions modulate this activity by shifting its pH optimum to physiological pH. This effect is not observed when p-nitrophenylphosphate is used as a substrate. Phosphoserine and phosphothreonine are not hydrolyzed under the same conditions.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland. agata@bf.uni.wroc.pl
authors.forename: A
authors.initials: A
authors.lastname: Szalewicz
authors.forename: B
authors.initials: B
authors.lastname: Strzelczyk
authors.forename: A
authors.initials: A
authors.lastname: Kubicz
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002413:Cations, Divalent; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008670:Metals / Q000494:pharmacology*; D019000:Phosphotyrosine / Q000378:metabolism*; D011893:Rana esculenta
chemical_list: D002413:Cations, Divalent; D008670:Metals; D019000:Phosphotyrosine; D000135:Acid Phosphatase