[Contents of inositol phosphates and response to phenylephrine in aorta from both young and old rats].
The changes of contents of inositol phosphates in Phenylephrine stimulated contraction of aorta from both 3 month and 20 month Wistar rats were studied by means of Bioassay method, and [3H]-myo-inositol labelling, ion exchange chromatography. The results were as follows: (1) In both resting and PE-activated arteries, IP took the greatest part (67%-83%) in total amount of inositol phosphates and the contents of IP, IP2 and IP3 were significantly higher in 20 month rat aorta than that in 3 month rat. (2) There is an age-related reduction in aortic contraction in Ca(2+)-free buffer and Ca(2+)-containing buffer. These results suggest that there is an age-related difference in IP metabolism pattern and pathway, and that IP metabolism in old rat is obviously active, but the Vascular response is apparently low.
Wang
S
S
Institute of Basic Medical Sciences, CAMS, Beijing.
Wen
Y
Y
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000367:Age Factors; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000316:Adrenergic alpha-Agonists; D007295:Inositol Phosphates; D010656:Phenylephrine; D015544:Inositol 1,4,5-Trisphosphate