Cell surface detection of HLA-E gene products with a specific monoclonal antibody.
An HLA-E-specific monoclonal antibody (mAb) was obtained by immunization of human beta2-microglobulin transgenic mice (M-TGM) with spleen cells from double transgenic mice expressing HLA-E molecules (EM-TGM). This mAb, designated V16, specifically recognizes in flow cytometry analysis the HLA-E expressing mouse cells, whereas it does not bind to mouse cells expressing various HLA class I molecules (HLA-A2, -A3, -A11, -A26, A29, -B7, -B27, -Cw3, -Cw7, and HLA-G). V16 mAb binds efficiently to human EBV-infected B lymphocytes, PHA blasts and PBL, thus establishing the surface expression of HLA-E in vivo on these cells.
Pacasova
R
R
Mouse Immunogenetics, U462 INSERM, Institute of Hematology, Saint-Louis Hospital, Paris, France.
Martinozzi
S
S
Boulouis
H J
HJ
Szpak
Y
Y
Ulbrecht
M
M
Sigaux
F
F
Weiss
E H
EH
Pla
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002462:Cell Membrane / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis* / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000276:immunology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D000097625:HLA-E Antigens
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens