Dual-colour FISH analysis to characterize a marker chromosome in cytochrome P450 2B1 recombinant V79 Chinese hamster cells.
Recombinant V79 Chinese hamster cell lines have been constructed for the expression of useful functions, e.g. cytochromes P450, in order to study metabolism-dependent toxicity. Recombinant cell lines are derived as single clones upon gene transfer and selection from the parental V79 cell line. It is of fundamental importance for a reliable application of the recombinant cell lines to show that the original biological characteristics of the parental line are maintained. As part of these efforts, fluorescence in situ hybridization with Chinese hamster chromosome-specific DNA libraries was performed in order to identify the origin of the chromosomes from which a marker chromosome present in the recombinant cell line V79MZr2B1 was derived, which could not be identified by standard cytogenetic techniques.
Simi
S
S
Genetica e Biochimica Tossicologica, Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy. s.simi@imd.pi.cnr.it
Xiao
Y
Y
Campagna
M
M
Doehmer
J
J
Darroudi
F
F
D000818:Animals; D016466:CHO Cells; D002871:Chromosome Banding; D006224:Cricetinae; D019362:Cytochrome P-450 CYP2B1 / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D011994:Recombinant Proteins / Q000235:genetics*
D005819:Genetic Markers; D011994:Recombinant Proteins; D019362:Cytochrome P-450 CYP2B1