Bactericidal activity of photocatalytic TiO(2) reaction: toward an understanding of its killing mechanism.
When titanium dioxide (TiO(2)) is irradiated with near-UV light, this semiconductor exhibits strong bactericidal activity. In this paper, we present the first evidence that the lipid peroxidation reaction is the underlying mechanism of death of Escherichia coli K-12 cells that are irradiated in the presence of the TiO(2) photocatalyst. Using production of malondialdehyde (MDA) as an index to assess cell membrane damage by lipid peroxidation, we observed that there was an exponential increase in the production of MDA, whose concentration reached 1.1 to 2.4 nmol. mg (dry weight) of cells(-1) after 30 min of illumination, and that the kinetics of this process paralleled cell death. Under these conditions, concomitant losses of 77 to 93% of the cell respiratory activity were also detected, as measured by both oxygen uptake and reduction of 2,3,5-triphenyltetrazolium chloride from succinate as the electron donor. The occurrence of lipid peroxidation and the simultaneous losses of both membrane-dependent respiratory activity and cell viability depended strictly on the presence of both light and TiO(2). We concluded that TiO(2) photocatalysis promoted peroxidation of the polyunsaturated phospholipid component of the lipid membrane initially and induced major disorder in the E. coli cell membrane. Subsequently, essential functions that rely on intact cell membrane architecture, such as respiratory activity, were lost, and cell death was inevitable.
Maness
P C
PC
The National Renewable Energy Laboratory, Golden, Colorado 80401-3393, USA. pinching_maness@nrel.gov
Smolinski
S
S
Blake
D M
DM
Huang
Z
Z
Wolfrum
E J
EJ
Jacoby
W A
WA
D004203:Disinfection / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D008027:Light; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D010101:Oxygen Consumption; D017319:Photosensitizing Agents / Q000494:pharmacology* / Q000528:radiation effects; D014025:Titanium / Q000494:pharmacology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D017319:Photosensitizing Agents; C009495:titanium dioxide; D008315:Malondialdehyde; D014025:Titanium