Technique for harvesting keratolimbal allografts from corneoscleral buttons.
OBJECTIVE
To report a method for obtaining thin keratolimbal lenticules from cadaveric corneoscleral buttons for corneal epithelial stem cell allografts.


METHODS
A standard silicone orbital sizing sphere and three 25-gauge needles were employed to fix the corneoscleral button to facilitate excision of keratolimbal lenticules.


RESULTS
Limbal stem cells in the form of keratolimbal lenticules were obtained without difficulty.


CONCLUSIONS
With this technique, thin keratolimbal lenticules of good quality can be obtained efficiently, inexpensively, and without specialized instruments.
Mannis
M J
MJ
Department of Ophthalmology, University of California, Davis, Sacramento, USA. mjmannis@ucdavis.edu
McCarthy
M
M
Izquierdo
L
L
D002102:Cadaver; D003315:Cornea; D016039:Corneal Transplantation; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D016850:Limbus Corneae / Q000166:cytology*; D015203:Reproducibility of Results; D012590:Sclera; D013048:Specimen Handling / Q000295:instrumentation*; D014019:Tissue Donors; D014184:Transplantation, Homologous