Effect of hyaluronic acid on migration, proliferation and alpha-smooth muscle actin expression by cultured rat and human fibroblasts.
Hyaluronic acid represents a major constituent of the extracellular matrix and influences numerous cellular processes. We have studied the effect of this molecule on migration, proliferation and alpha-smooth muscle actin expression by rat and human cultured fibroblasts. Depending on the tissue origin of the fibroblasts, hyaluronic acid induced, inhibited or did not affect fibroblast migration, whereas proliferation was never affected. Alpha-smooth muscle actin expression was inhibited by hyaluronic acid in all types of human and rat fibroblasts studied, but the inhibition was stronger and dose dependent for subcutaneous fibroblasts and for those recovered from Dupuytren's nodules. This property of inhibiting alpha-smooth muscle actin expression makes hyaluronic acid a good candidate to exert antifibrotic activity.
Andreutti
D
D
Department of Pathology, University of Geneva-CMU, Switzerland.
Geinoz
A
A
Gabbiani
G
G
D000199:Actins / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000378:metabolism; D051381:Rats
D000199:Actins; D006820:Hyaluronic Acid