Inhibition of immunoglobulin E production by Poncirus trifoliata fruit extract.
Immunoglobulin E (IgE) is the principal immunoglobulin involved in immediate hypersensitivities and chronic allergic diseases. The effect of an aqueous extract of Poncirus trifoliata (L) Raf. (Rutaceae) fruits (PTFE) on in vivo and in vitro IgE production was investigated. PTFE dose-dependently inhibited the active systemic anaphylaxis and serum IgE production induced by immunization with ovalbumin, Bordetella pertussis toxin and aluminum hydroxide gel. PTFE strongly inhibited interleukin 4 (IL-4)-dependent IgE production by lipopolysaccharide-stimulated murine whole spleen cells. In the case of U266 human IgE-bearing B cells, PTFE also showed an inhibitory effect on the IgE production. These results suggest that PTFE has an anti-allergic activity by inhibition of IgE production from B cells.
Kim
H M
HM
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan-city, Chonbuk, South Korea. hmkim@med.wonkwang.ac.kr
Kim
H J
HJ
Park
S T
ST
D000536:Aluminum Hydroxide / Q000633:toxicity; D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D015847:Interleukin-4 / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D010047:Ovalbumin / Q000633:toxicity; D037342:Pertussis Toxin; D010936:Plant Extracts / Q000276:immunology / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000633:toxicity
D008070:Lipopolysaccharides; D010936:Plant Extracts; D010566:Virulence Factors, Bordetella; D015847:Interleukin-4; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide; D010047:Ovalbumin; D037342:Pertussis Toxin