title: An in vitro analysis of ecdysteroid-elicited cell death in the prothoracic gland of Manduca sexta.
abstract: The prothoracic glands of the tobacco hornworm, Manduca sexta, secrete the precursor of the insect molting hormone and normally undergo programmed cell death (PCD) during pupal-adult metamorphosis, between days 5 and 6 after pupation. This phenomenon can be elicited prematurely in vitro by the addition of 20-hydroxyecdysone (20E) to the gland cultures. To induce nuclear condensation in vitro in the glands from day-1 pupae, the effective dose range of 20E is 0.7-7 micrograms/ml and the minimum exposure period is 24 h. Prothoracic glands from different stages of pupal-adult development express different responsiveness to exogenous ecdysteroids. By utilizing terminal deoxynucleotidyl-transferase-mediated dUTP nick-end-labeling (TUNEL) and the apoptotic DNA laddering method together with transmission electron microscopy, it has been demonstrated that the ecdysteroid-induced cell death of the prothoracic glands occurs via not only apoptosis but also autophagy, i.e., the induced dying cells show both severe nuclear fragmentation and autophagic vacuole formation, characteristics typical of apoptotic and autophagic cell death. The composite data indicate that ecdysteroids regulate directly both apoptotic and autophagic mechanisms of PCD of the prothoracic glands.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Dai
authors.forename: L I
authors.initials: LI
authors.lastname: Gilbert
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004441:Ecdysterone / Q000494:pharmacology*; D004702:Endocrine Glands / Q000187:drug effects*; D020287:In Situ Nick-End Labeling; D018402:Manduca / Q000187:drug effects*; D008854:Microscopy, Electron
chemical_list: D004441:Ecdysterone; D004247:DNA