title: [Effects of calcium channel blockers and calmodulin inhibitors on the secretion of endothelin-1 in cultured endothelial cells].
abstract: OBJECTIVE
  The study is to investigate the effects of calcium channel blockers and calmodulin inhibitors on the secretion of endothelin-1 (ET-1) in cultured endothelial cells from human umbilical veins.
  
  
  RESULTS
  Results showed that calcium channel blockers verapamil (5.5 x 10(-6) mol/L, 5.5 x 10(-5) mol/L), diltiazen (2.4 x 10(-4) mol/L) and calmodulin inhibitors chlorpromazine (3.1 x 10(-5) mol/L, 3.1 x 10(-4) mol/L), berbamine (1.6 x 10(-5) mol/L, 1.6 x 10(-4) mol/L) significantly decreased medium ET-1 levels in cultured endothelial cells.
  
  
  CONCLUSIONS
  It was also indicated that extracellular calcium influx and calmodulin activity were necessary to the secretion of ET-1. In addition, nitroglycerine (2.2 x 10(-3) mol/L) remarkably reduced medium ET-1 levels in cultured endothelial cells, which suggested that nitric oxide might inhibit the secretion of ET-1.
authors.affiliation: PUMC Hospital, Beijing.
authors.forename: W
authors.initials: W
authors.lastname: Cao
authors.forename: Z
authors.initials: Z
authors.lastname: Zeng
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: Z
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology*; D004110:Diltiazem / Q000494:pharmacology; D019332:Endothelin-1 / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D005996:Nitroglycerin / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers; D002147:Calmodulin; D019332:Endothelin-1; D014700:Verapamil; D004110:Diltiazem; D005996:Nitroglycerin; D002746:Chlorpromazine; C027870:berbamine