Effects of cimetidine and its metal complexes on nitroblue tetrazolium and ferricytochrome c reduction by superoxide radicals.
The effects of cimetidine, a potent histamine-H2-receptor antagonist and a good -OH scavenger, on the kinetics of ferricytochrome c and NBT reduction by superoxide anions were studied. The drug dose-dependently inhibited ferricytochrome c and NBT reduction by O2- radicals, generated either in xanthine oxidase system or photochemically or directly by KO2. The inhibitory effect of cimetidine remained unchanged in the presence of catalase or mannitol. Cimetidine and its complexes with Cu(II) and Fe(III) ions inhibited ferricytochrome c and NBT reduction even when metal chelators were added to the reaction medium. The results suggest the reaction of cimetidine with O2-radicals.
Kirkova
M
M
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
Atanassova
M
M
Russanov
E
E
D002927:Cimetidine / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D003574:Cytochrome c Group / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D008670:Metals / Q000737:chemistry / Q000494:pharmacology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D013481:Superoxides / Q000494:pharmacology*
D003574:Cytochrome c Group; D008670:Metals; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D008353:Mannitol; D003300:Copper; D002927:Cimetidine; D004492:Edetic Acid