Isolation, nucleotide sequence, and physiological relevance of the gene encoding triose phosphate isomerase from Kluyveromyces lactis.
Lack of triose phosphate isomerase activity (TIM) is of special interest because this enzyme works at an important branch point of glycolytic flux. In this paper, we report the cloning and sequencing of the Kluyveromyces lactis gene encoding TIM. Unlike Saccharomyces cerevisiae DeltaTPI1 mutants, the K. lactis mutant strain was found to be able to grow on glucose. Preliminary bioconversion experiments indicated that, like the S. cerevisiae TIM-deficient strain, the K. lactis TIM-deficient strain is able to produce glycerol with high yield.
Compagno
C
C
Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, Università degli Studi di Milano, 20133 Milan, Italy. compagno@imiucca.csi.inimi.it
Boschi
F
F
Daleffe
A
A
Porro
D
D
Ranzi
B M
BM
D015139:Blotting, Southern; D003001:Cloning, Molecular; D017353:Gene Deletion; D005798:Genes, Bacterial; D005990:Glycerol / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014305:Triose-Phosphate Isomerase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D014305:Triose-Phosphate Isomerase; D005990:Glycerol