title: Sandwich enzyme-linked immunosorbent assay for plasma cholesteryl ester transfer protein concentration.
abstract: OBJECTIVE
  Cholesteryl ester transfer protein (CETP) mediates the transfer of HDL cholesterol to apoB-containing lipoproteins. Its mass and activity are increased in several pro-atherogenic conditions. The objective of this study is to develop a cost- and time-effective sandwich ELISA for plasma CETP concentration.
  
  
  METHODS
  Monoclonal anti-CETP, TP20, was used as the capture antibody, while the other biotinylated monoclonal anti-CETP, TP2, was used for detection. The results were expressed in an arbitrary unit, ng biotin-TP2 bound per microl plasma. Plasma CETP concentrations, activities and their relationship were assessed in 35 IDDM children.
  
  
  RESULTS
  The assay had an intra-assay CV of 8.75% and an inter-assay CV under 10%. Plasma CETP concentration of these subjects ranged from 0.36-1.89 ng biotin-TP2/microL. CETP concentration was significantly correlated with CETP activity (r = 0.51, p < 0.01).
  
  
  CONCLUSIONS
  The sandwich ELISA we have developed carried sufficient sensitivity for assaying plasma CETP concentration in human.
authors.affiliation: OSU Nutrition Program, Ohio State University, Columbus, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Chang
authors.forename: T K
authors.initials: TK
authors.lastname: Tso
authors.forename: J T
authors.initials: JT
authors.lastname: Snook
authors.forename: W B
authors.initials: WB
authors.lastname: Zipf
authors.forename: R A
authors.initials: RA
authors.lastname: Lozano
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000097:blood* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D053480:Cholesterol Ester Transfer Proteins; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D005260:Female; D006023:Glycoproteins; D006801:Humans; D008297:Male; D012015:Reference Standards
chemical_list: D000911:Antibodies, Monoclonal; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins