Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains.
Brucella abortus vaccine strain RB51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. The genetic mutations that are responsible for the roughness and the attenuation of strain RB51 have not been identified until now. Also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain RB51 from its parent strain 2308 is available. In the present study, we demonstrate that the wboA gene encoding a glycosyltransferase, an enzyme essential for the synthesis of O antigen, is disrupted by an IS711 element in B. abortus vaccine strain RB51. Exploiting this feature, we developed a PCR assay that distinguishes strain RB51 from all other Brucella species and strains tested.
Vemulapalli
R
R
Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061-0342, USA.
McQuiston
J R
JR
Schurig
G G
GG
Sriranganathan
N
N
Halling
S M
SM
Boyle
S M
SM
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines; D001483:Base Sequence; D002003:Brucella abortus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D017347:Brucella melitensis / Q000235:genetics / Q000302:isolation & purification; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000517:prevention & control; D002417:Cattle; D017931:DNA Primers; D004251:DNA Transposable Elements; D016695:Glycosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D017931:DNA Primers; D004251:DNA Transposable Elements; D019081:O Antigens; D016695:Glycosyltransferases