Temporal expression of the matrix metalloproteinase MMP-2 correlates with fibronectin immunoreactivity during the development of the vascular system in the chick embryo chorioallantoic membrane.
In this study we have examined in the chick embryo chorioallantoic membrane (ChAM) the expression of the matrix metalloproteinase 2 (MMP-2) and have correlated this parameter with the expansion of the ChAM vasculature and with the expression of 3 extracellular matrix components (fibronectin, laminin and type IV collagen), which differentially modulate angiogenesis. In the early phases of ChAM development, between d 6 and d 8 of incubation, when the increase of the ChAM vasculature is maximal, higher values of MMP-2 and, respectively, of fibronectin immunoreactive area, are detectable. These results indicate that MMP-2 activity and fibronectin expression are 2 strictly related components of angiogenesis occurring in vivo.
Ribatti
D
D
Institute of Human Anatomy, Histology and Embryology, University of Bari Medical School, Italy. ribatti@anatomia.uniba.it
Nico
B
B
Vacca
A
A
Iurlaro
M
M
Roncali
L
L
D000818:Animals; D002642:Chick Embryo / Q000378:metabolism*; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000098:blood supply* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D018093:Gelatinases / Q000032:analysis / Q000378:metabolism*; D005865:Gestational Age; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000032:analysis / Q000378:metabolism*; D018919:Neovascularization, Physiologic
D005353:Fibronectins; D007797:Laminin; D003094:Collagen; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2