title: Gender differences in renal growth and function after uninephrectomy in adult rats.
abstract: BACKGROUND
  It is known that compensatory renal growth (CRG) following unilateral nephrectomy (UNX) increases both the size of the kidney and its functional capacity; however, few studies have investigated whether differences in CRG exist between the sexes. Our study examined the sex-related differences in remnant kidney growth and function two months following UNX.
  
  
  METHODS
  Adult male and female Wistar rats underwent either left UNX or sham operation and recovered for 8 to 10 weeks. Another group of female rats underwent ovariectomy (Ox), with vehicle, estrogen, or testosterone replacement: two-weeks postsurgery animals underwent UNX and recovered for 8 to 10 weeks. Metabolic studies, acute renal function studies [response to acute saline volume expansion (2 to 4% of body wt) or phosphate (Pi) infusions in thyroparathyroidectomized rats (to determine the transport maximum (TmPi)], and renal morphology were assessed at the end of the experimental period.
  
  
  RESULTS
  Two months post-UNX, male remnant kidneys grew 114+/-7% of their excised kidney weight (KW), whereas female remnant kidneys grew only 57+/-4% (P<0.05). There was a significant increase in the glomerular volume of male remnant kidneys (126.2+/-13.4%, P<0.001) compared with control kidney volume, whereas there was no change in glomerular volume in female remnant kidneys (20.2+/-16.1%, P = NS). There was also glomerular and tubular damage in the male remnant kidneys, whereas female remnant kidneys were intact. Studies in Ox female rats supplemented with gonadal steroids determined that testosterone is the driving force for the enhanced remnant kidney growth and glomerular hypertrophy. Renal function studies determined that UNX males had significantly higher glomerular filtration rates (GFRs) than UNX females, although the GFR/single KW was not different between the sexes, indicating a proportional increase in GFR. Basal urinary sodium excretion and urine flow rates were significantly higher in anesthetized UNX rats than their sham-operated controls, and urinary sodium excretion and urine flow rates in UNX males were significantly higher than in UNX females. Both male and female UNX rats responded to volume expansion with an exaggerated initial sodium and urine excretion compared with their controls. Phosphate handling was not altered in UNX male rats; however, UNX female rats had increases in fractional Pi excretion that were associated with significant reductions in the maximum capacity for Pi reabsorption (2.10+/-0.07 vs. 3.43+/-0.24 micromol/ml GFR in female controls, P<0.0001). This difference was also observed in Ox rats treated with estrogen and testosterone (2.31+/-0.07 vs. 3.12+/-0.11 micromol/ml GFR, P<0.0007).
  
  
  CONCLUSIONS
  These findings indicate that sexual dimorphism exists in remnant kidney growth and function two months following UNX. Indeed, morphological abnormalities and impairment in renal phosphate handling are affected by gonadal steroids by two-months post-UNX. The fact that renal Pi transport was reduced in female but not male UNX rats may also have important implications during periods of high metabolic demand for phosphate in the female.
authors.affiliation: Department of Physiology and Biophysics, Georgetown University School of Medicine, Washington DC 20007, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Mulroney
authors.forename: C
authors.initials: C
authors.lastname: Woda
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.forename: C
authors.initials: C
authors.lastname: Pesce
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology; D005260:Female; D006984:Hypertrophy; D007668:Kidney / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology*; D008297:Male; D009392:Nephrectomy; D010052:Ovariectomy; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D012727:Sex Characteristics; D013739:Testosterone / Q000494:pharmacology / Q000502:physiology; D013997:Time Factors
chemical_list: D010710:Phosphates; D013739:Testosterone; D004958:Estradiol