New polymer-based prepacked column for the reversed-phase liquid chromatographic separation of peptides over the pH range 2-12.
The aim of this paper was to investigate the properties of a new column, Source 5RPC, for the separation of peptides at pH 2, 4.5, 7, 9 and 12 and to compare this product with similar polymer-based products available on the market. All columns were prepacked with 5 microm polystyrene-divinylbenzene polymer bead matrices and had dimensions of 150x4.6 mm I.D. Separations of angiotensin peptides were achieved on these columns using different equilibration solvents in the pH range 2-12 and elution with acetonitrile gradients. The separation of the peptide mixture obtained on Source 5RPC column was compared with that of two other commercially available polymer-based matrices. Method scouting and optimisation were carried out using the novel chromatography system, AKTA purifier.
Mohammad
J
J
Amersham Pharmacia Biotech AB, Uppsala, Sweden. jamil.mohammad@eu.apbiotech.com
Jäderlund
B
B
Lindblom
H
H
D000809:Angiotensins / Q000031:analogs & derivatives / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet
D000809:Angiotensins