Speeds of actin translocation in vitro by myosins extracted from single rat muscle fibres of different types.
As skeletal muscle fibres mostly express a single myosin isoform, they are a potential source of pure myosin isoforms. A technique is described that allows extraction and identification of pure myosin isoforms from single fibres, and testing of such myosins in an in vitro motility assay (IVMA). The results show that the extraction procedure does not alter myosin function and support the view that single fibres are reliable sources of purified myosin isoforms for IVMA.
Canepari
M
M
Institute of Human Physiology, University of Pavia, Italy. canepari@unipv.it
Rossi
R
R
Pellegrino
M A
MA
Reggiani
C
C
Bottinelli
R
R
D000199:Actins / Q000502:physiology*; D000818:Animals; D066298:In Vitro Techniques; D018715:Microscopy, Video; D009068:Movement / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology; D015879:Myosin Subfragments / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D020033:Protein Isoforms / Q000032:analysis; D011817:Rabbits; D051381:Rats
D000199:Actins; D015879:Myosin Subfragments; D020033:Protein Isoforms