title: Validation of a flow cytometric assay detecting in vitro basophil activation for the diagnosis of muscle relaxant allergy.
abstract: BACKGROUND
  Anaphylactic reactions during anesthesia are mainly the result of muscle-relaxant (MR) drugs. Skin tests, serologic detection of specific IgE, and in vitro leukocyte histamine release are used to investigate MR allergy.
  
  
  OBJECTIVE
  We describe a new assay that is based on the detection by flow cytometry of the altered expression of plasma membrane molecules of MR-activated basophils.
  
  
  METHODS
  For this assay, which we have named the BASIC assay, basophils are incubated in vitro with MR, after which they are fixed and then triple labeled with fluorescein-conjugated anti-CD63, tandem dye R-phycoerythrin-cyanin 5.1 conjugated anti-CD45, and R-phycoerythrin conjugated anti-IgE. The resulting B asophils' A ltered S urface I mmunofluorescence is detected by flow C ytometry (BASIC).
  
  
  RESULTS
  Forty-one patients who had an allergic reaction during general anesthesia and 23 control subjects without such a history were studied. All included subjects' basophils were tested in the BASIC assay with at least 4 MR: suxamethonium, gallamine, vecuronium, and pancuronium. After reaction of the basophils of the MR-allergic patients with MRs, increased surface expression of CD63 and CD45 and decreased expression of IgE were detected. Increased expression of CD63 was observed most frequently and it was stronger than the alteration of the 2 other markers. Cross-reactivity between MRs commonly occurred. MRs diluted 10(-1) activate the basophils of the control subjects, suggesting that at relatively high concentrations MRs are also nonspecific basophil activators.
  
  
  CONCLUSIONS
  In the diagnosis of MR allergy, the BASIC assay has a good specificity but a low sensitivity, and it correlates strongly with skin test results. It is currently appraised for the diagnosis of anaphylactic reaction induced by other classes of drugs.
authors.affiliation: Service d'Immunologie et d'Hématologie Biologique, Hôpital Rothschild Assistance Publique, Hôpitaux de Paris, Centre Hospital-Universitaire Saint-Antoine, Université Pierre et Marie Curie, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Abuaf
authors.forename: B
authors.initials: B
authors.lastname: Rajoely
authors.forename: E
authors.initials: E
authors.lastname: Ghazouani
authors.forename: D A
authors.initials: DA
authors.lastname: Levy
authors.forename: C
authors.initials: C
authors.lastname: Pecquet
authors.forename: H
authors.initials: H
authors.lastname: Chabane
authors.forename: F
authors.initials: F
authors.lastname: Leynadier
mesh_terms: D000328:Adult; D000368:Aged; D000707:Anaphylaxis / Q000139:chemically induced / Q000175:diagnosis*; D001491:Basophils / Q000502:physiology*; D004342:Drug Hypersensitivity / Q000209:etiology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009125:Muscle Relaxants, Central / Q000009:adverse effects* / Q000276:immunology*; D010197:Pancuronium / Q000276:immunology; D015203:Reproducibility of Results; D012882:Skin Tests; D014673:Vecuronium Bromide / Q000276:immunology
chemical_list: D009125:Muscle Relaxants, Central; D014673:Vecuronium Bromide; D010197:Pancuronium