The use of soybean agglutinin (SBA) for bone marrow (BM) purging and hematopoietic progenitor cell enrichment in clinical bone-marrow transplantation.
Soybean agglutin (SBA) is a plant lectin that has been used to fractionate bone marrow cells. It binds bone marrow mononuclear cells, including mature myeloid, erythroid, and lymphoid cells, but has very low binding affinity and no toxic effect to the human hematopoietic cells. In this article we describe the possibilities of enriching bone-marrow-derived CD34+ hematopoietic progenitor cells using SBA. As the method is simple and elegant. SBA is of vast importance to the field of clinical bone marrow transplantation.
Nagler
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Bone Marrow Transplantation Department, Hadassah University Hospital, Jerusalem, Israel. nagler@hadassah.org.il
Morecki
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Slavin
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S
D000328:Adult; D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D016465:Bone Marrow Purging / Q000379:methods*; D016026:Bone Marrow Transplantation / Q000379:methods*; D001943:Breast Neoplasms / Q000628:therapy; D002051:Burkitt Lymphoma / Q000628:therapy; D002986:Clinical Trials as Topic; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008875:Middle Aged; D037121:Plant Lectins; D030262:Soybean Proteins; D013601:T-Lymphocytes / Q000187:drug effects; D013997:Time Factors; D010835:Phytohemagglutinins
D018952:Antigens, CD34; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins