title: Effect of oligomer length and base substitutions on the cytotoxic activity and specific nuclear protein recognition of GTn oligonucleotides in the human leukemic CCRF-CEM cell line.
abstract: We have identified phosphodiester oligonucleotides composed of G and T bases, named GTn, which are able to inhibit the cellular growth of human cancer cell lines by recognising specific nuclear proteins. We demonstrated that GTn oligonucleotides require a length of at least 20 nucleotides in order to exert a significant cytotoxic effect and to retain the specific protein binding ability. In addition, we found that GTn cytotoxicity was lost when A or C bases were introduced at either 3' and 5' end or within the GTn sequences.
authors.affiliation: Department of Biomedical Sciences and Technologies, University of Udine, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Morassutti
authors.forename: B
authors.initials: B
authors.lastname: Dapas
authors.forename: B
authors.initials: B
authors.lastname: Scaggiante
authors.forename: G
authors.initials: G
authors.lastname: Paroni
authors.forename: L
authors.initials: L
authors.lastname: Xodo
authors.forename: F
authors.initials: F
authors.lastname: Quadrifoglio
mesh_terms: D001704:Biopolymers; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D001704:Biopolymers; D009687:Nuclear Proteins; D009841:Oligonucleotides