Expression of adhesion molecules and effect of disodium cromoglycate treatment in asthmatics.
Allergic processes are complex disorders in which inflammatory and immunological mechanisms are involved. Many studies indicate that the adhesion molecules are upregulated in allergic inflammation, and play a critical role in the pathogenesis of allergic inflammation. Modulation of the expression of adhesion molecules may provide a potential new target for therapeutic intervention in allergic diseases. In the present study the changes expression of adhesion molecules CD11a, CD18 (LFA-1), CD54 (ICAM-1) and L-selectin (CD62L) and VLA-4 (CD49d) were analysed by flow cytometry. Serum concentrations of soluble ICAM-1, VCAM-1 and soluble low affinity receptor for IgE concentrations sCD23 were measured by ELISA in atopic patients with mild asthma before and after treatment by disodium cromoglycate (DSCG). The most significant finding was a significant decrease of ICAM-1 expression on monocytes and CD49d on monocytes and lymphocytes as well as an increase of L-selectin expression on monocytes after treatment with DSCG, without any associated effect on CD11a and CD18. The levels of soluble ICAM-1 and VCAM-1 were not changed, only the levels of soluble CD23 that plays a regulatory role in ongoing IgE production, were decreased in asthmatic patients after the treatment with DSCG. These results suggest that DSCG diminishes cell activation.
Jahnová
E
E
Institute of Preventive and Clinical Medicine, Department of Clinical Immunology, Bratislava, Slovak Republic.
Horváthová
M
M
Gazdík
F
F
D000328:Adult; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use*; D001249:Asthma / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000097:blood*; D004205:Cromolyn Sodium / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D009000:Monocytes / Q000378:metabolism; D017455:Receptors, IgE / Q000097:blood; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood
D018927:Anti-Asthmatic Agents; D015815:Cell Adhesion Molecules; D017455:Receptors, IgE; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D004205:Cromolyn Sodium