title: Human sperm penetration in different media.
abstract: Sperm penetration into bovine cervical mucus and hen egg white using capillary tube penetration was investigated to verify the suitability of the capillary tube penetration test with hen egg white as a test of human sperm function. Semen samples from 50 consecutive patients were used for penetration tests and spermatozoa of a further 10 semen samples were penetrated into bovine cervical mucus and hen egg white for special motility assessment by computer-assisted motility analysis. Penetration tests revealed the well-known different ability of spermatozoa to penetrate into cervical mucus and a different penetration of spermatozoa into egg white for two nearly equal groups (n = 24 and n = 26, respectively). One group showed penetration comparable with cervical mucus and one group a very fast penetration up to the limit of the scale of measurement. Motility assessment of spermatozoa that penetrated into cervical mucus and egg white revealed significant differences in straight-line velocity, linearity and lateral head displacement. The number of spermatozoa selected actively during the penetration procedure was significantly higher in cervical mucus than in hen egg white. Spermatozoa selected by bovine cervical mucus and hen egg white exhibited a different motility pattern. There was significantly better linearity and less lateral head displacement in egg white than in cervical mucus. Sperm penetration into hen egg white appeared to be influenced by different sources of egg white.
authors.affiliation: Dermatological Clinic, School of Medicine, Charité, Humboldt-University Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Engel
authors.forename: R
authors.initials: R
authors.lastname: Petzoldt
mesh_terms: D000818:Animals; D002417:Cattle; D002582:Cervix Mucus / Q000502:physiology; D002645:Chickens; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010063:Ovum / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*