An archaeal aminoacyl-tRNA synthetase missing from genomic analysis.
The complete genomic sequencing of Methanococcus jannaschii cannot identify the gene for the cysteine-specific member of aminoacyl-tRNA synthetases. However, we show here that enzyme activity is present in the cell lysate of M. jannaschii. The demonstration of this activity suggests a direct pathway for the synthesis of cysteinyl-tRNA(Cys) during protein synthesis.
Hamann
C S
CS
Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Sowers
K R
KR
Lipman
R S
RS
Hou
Y M
YM
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D019766:Genes, Archaeal; D016678:Genome; D007700:Kinetics; D017017:Methanococcus / Q000201:enzymology* / Q000235:genetics*; D013816:Thermodynamics
D000604:Amino Acyl-tRNA Synthetases; C019975:cysteinyl-tRNA synthetase