title: The relaxant effects of parathyroid hormone(1-34) and parathyroid hormone-related protein(1-34) on ovine reticulo-ruminal smooth muscle in vivo.
abstract: The motility of the reticulo-rumen has been measured in trained, conscious sheep using inflated balloons temporarily introduced to selected regions of that forestomach. The frequency and amplitude of the contractions of the reticulum and both the A and B waves of contraction of the rumen were measured under the same conditions before, during and after the administration of an i.v. bolus of either parathyroid hormone (PTH(1-34)) or PTH-related protein (PTHrP(1-34)) followed by its i.v. infusion. These two peptides are known to share a common receptor in other organs, e.g. the kidney. In this study they both showed an inhibitory effect on reticulo-ruminal motility. The effect of PTHrP(1-34) on the rate of ruminal blood flow was also examined and a significant reduction observed, after a transient increase. The secretion of endogenous PTH(1-34) was stimulated by a 32% reduction in the plasma calcium ion concentration induced by an i.v. infusion of sodium citrate. Associated with this were significant reductions in reticulo-ruminal motility, e.g. the reduction in the mean amplitude of the reticular contractions reflected the reduction in plasma calcium ion concentration. When the PTH(1-34)/PTHrP(1-34) receptor was blocked with [Asn10,Leu11,D-Trp12]PTHrP(7-34) before and during the induction of hypocalcaemia, all but one of the parameters of reticulo-ruminal motility were normalized. Indeed, by the day following the administration of this blocking agent, all these parameters had returned to their normal range. It is concluded that stimulation of the PTH(1-34)/PTHrP(1-34) receptor in reticulo-ruminal smooth muscle reduces the motility of this tissue and may play a role in the depression of motility of the digestive tract which is characteristic of clinical milk fever in the dairy cow.
authors.affiliation: Institute of Biological Sciences, University of Wales, Aberystwyth, UK.
authors.forename: A D
authors.initials: AD
authors.lastname: Care
authors.forename: S K
authors.initials: SK
authors.lastname: Abbas
authors.forename: J
authors.initials: J
authors.lastname: Harmeyer
authors.forename: R
authors.initials: R
authors.lastname: Boivin
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D006996:Hypocalcemia / Q000097:blood / Q000139:chemically induced; D009126:Muscle Relaxation / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects*; D010281:Parathyroid Hormone / Q000494:pharmacology; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D012159:Reticulum / Q000502:physiology*; D012417:Rumen / Q000502:physiology*; D012756:Sheep; D019379:Teriparatide / Q000494:pharmacology*
chemical_list: D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D011506:Proteins; C431128:parathyroid hormone-related protein (7-34); C111392:parathyroid hormone-related protein (7-34)amide, Asn(10), Leu(11)-; D019379:Teriparatide; C054296:parathyroid hormone-related protein (1-34); D002118:Calcium