Empirical relationship between chiral selectivity and mobile phase modifier properties.
An empirical relationship was derived which relates properties of the mobile phase modifier to the chiral selectivity factor for a given analyte/chiral selector combination. Using carbon dioxide and heptane-based mobile phases, the effect of various mobile phase modifiers on Pirkle-type stationary phases may be accurately modeled using a two-parameter equation. Similar results are obtained using cellulosic stationary phases with carbon dioxide-based mobile phases. Modeling separations performed using heptane-based mobile phases with cellulosic stationary phases were not successful. The predictive ability of this modeling approach was demonstrated using novel modifiers and chiral analytes.
Blackwell
J A
JA
Dupont Pharmaceuticals Company, Chemical Process Research and Development, Chambers Works, Deepwater, NJ 08023, USA. john.a.blackwell@dupontpharma.com
Stringham
R W
RW
Xiang
D
D
Waltermire
R E
RE
D002853:Chromatography, Liquid / Q000379:methods*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D012997:Solvents