Captopril increased mitochondrial coenzyme Q10 level, improved respiratory chain function and energy production in the left ventricle in rabbits with smoke mitochondrial cardiomyopathy.
The aim of the study was to show whether the ACE inhibitor captopril is able to protect the heart against the deleterious effect of passive cigarette smoking on left ventricular mitochondria. Four groups of rabbits were investigated: control (C), passive smoking of three cigarettes twice daily/30 minutes (S), control + captopril (7.5 mg/kg body weight twice daily) (Cap), and smoking + captopril (SCap) as in group 2 and 3. Three weeks lasting passive smoking impaired oxidative phosphorylation, diminished cytochrome oxidase activity and increased the mitochondrial F1-ATPase protein concentration. Moreover, the level of coenzyme Q10 (CoQ10) and coenzyme Q9 were decreased. Simultaneous treatment with captopril prevented partly the decrease of CoQ10 level, deterioration of oxidative phosphorylation, diminution of cytochrome oxidase activity and enhancement of F1-ATPase level. We conclude that captopril protected the myocardium against the harmful effect of passive smoking in rabbits.
Gvozdjáková
A
A
Pharmacobiochemical Laboratory of Medical Faculty, Comenius University, Bratislava, Slovak Republic.
Simko
F
F
Kucharská
J
J
Braunová
Z
Z
Psenek
P
P
Kyselovic
J
J
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000627:therapeutic use; D000818:Animals; D002216:Captopril / Q000627:therapeutic use*; D009202:Cardiomyopathies / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D003067:Coenzymes; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D006352:Heart Ventricles / Q000648:ultrastructure; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D017240:Mitochondrial Myopathies / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011817:Rabbits; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D003067:Coenzymes; D014028:Tobacco Smoke Pollution; D014451:Ubiquinone; D002216:Captopril; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases; C024989:coenzyme Q10