title: LIAISON Ferritin--an automated chemiluminescent immunoassay for the determination of Ferritin.
abstract: An immunoassay utilizing chemiluminescence and paramagnetic particles has been developed for the new fully automated random access LIAISON immunoanalyzer. The LIAISON Ferritin assay is a two-site immunoluminometric one-step assay using two highly specific monoclonal antibodies. A specially designed unique Reagent Integral contains all specific reagents. The cooled reagent compartment guarantees an on-board stability of at least 4 weeks. The assay works with a 2-point calibrated master curve. To perform, the assay, 10 microliters sample is added to 300 microliters tracer and 20 microliters antibody-coated magnetic particles. After 10 min incubation the particles are separated, washed and the chemiluminescent signal is generated. The time to first result is only 15 min. The assay with a unique extended standard range up to 3,000 ng/ml shows no high dose hook effect up to 150,000 ng/ml (spiked sera). The cross-reactivity to human liver ferritin is 100%, to human spleen ferritin 66% and to human heart ferritin 8%. Precision (within-run < 3%; between-run < 5%), linearity, recovery and sensitivity (< 0.2 ng/ml) are excellent. The assay shows a very good correlation to LlA-mat Ferritin (r = 0.994). In summary the LIAISON Ferritin run on the new LIAISON immunoanalyzer and is a very rapid and accurate method rapidly providing reliable results.
authors.forename: B
authors.initials: B
authors.lastname: König
authors.forename: M
authors.initials: M
authors.lastname: Oed
authors.forename: A
authors.initials: A
authors.lastname: Kunz
authors.forename: R
authors.initials: R
authors.lastname: Schlett
authors.forename: M
authors.initials: M
authors.lastname: Mack
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002138:Calibration; D005260:Female; D005293:Ferritins / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008163:Luminescent Measurements; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012727:Sex Characteristics
chemical_list: D005293:Ferritins