Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion.
We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 microg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or follicle-stimulating hormone but did increase prolactin from 19.6 +/- 5.9 ng/ml in the sense group to 52.9 +/- 9.6 ng/ml in the antisense group. The plasma hormones were measured by radioimmunoassay and the values are reported as mean +/- SEM. These data suggest that endogenous neuropeptide Y in the medial preoptic area has an inhibitory action on prolactin secretion through Y1 receptors.
Silveira
N A
NA
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade de Goiás, Goiânia, GO, Brasil.
Franci
C R
CR
D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D011301:Preoptic Area / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000235:genetics* / Q000502:physiology
D016372:RNA, Antisense; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone