title: Comparison between collision induced dissociation of electrosprayed protonated peptides in the up-front source region and in a low-energy collision cell.
abstract: A systematic comparison between the up-front Collision induced dissociation (CID) mass spectra and low-energy CID tandem mass spectra from twenty-one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up-front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up-front CID and low-energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated.
authors.affiliation: Bijvoet Centre for Biomolecular Research, Department of Mass Spectrometry, Utrecht University, F.A.F.C Wentgebouw, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. dongen@voeding.tno.nl
authors.forename: W D
authors.initials: WD
authors.lastname: van Dongen
authors.forename: J I
authors.initials: JI
authors.lastname: van Wijk
authors.forename: B N
authors.initials: BN
authors.lastname: Green
authors.forename: W
authors.initials: W
authors.lastname: Heerma
authors.forename: J
authors.initials: J
authors.lastname: Haverkamp
mesh_terms: D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D010455:Peptides