Comparison of two molecular techniques for the detection of avian reoviruses in formalin-fixed, paraffin-embedded chicken tissues.
Reverse transcription (RT) in situ polymerase chain reaction (PCR) and in situ hybridization (ISH) techniques were used to detect the sigma c-encoded gene of avian reovirus (ARV) in chicken tissue sections. The advantage of using in situ methods is to make more rapid and accurate diagnosis of ARV infections. The sensitivity of these two techniques were compared. Of the two techniques, the RT in situ PCR test was found to be more sensitive than ISH and provided the rapid, sensitive, and specific detection of ARV infections.
Liu
H J
HJ
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Taiwan, ROC. hjliu@mail.npust.edu.tw
Liao
M H
MH
Chang
C D
CD
Chen
J H
JH
Lin
M Y
MY
Tung
M C
MC
D000818:Animals; D002645:Chickens / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D017403:In Situ Hybridization / Q000662:veterinary; D015336:Molecular Probe Techniques / Q000662:veterinary; D018165:Orthoreovirus / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding / Q000662:veterinary*; D012088:Reoviridae Infections / Q000662:veterinary / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016610:Tissue Embedding / Q000662:veterinary*
D004279:DNA, Viral