Modulation of apo A-IV transcript levels and synthesis by n-3, n-6, and n-9 fatty acids in CACO-2 cells.
It has been postulated that apolipoprotein (apo) A-IV plays various significant roles in lipid transport and lipoprotein metabolism. Although it is controlled by fat feeding, so far little else is known about its regulation by specific fatty acids. In this study, we focused on the modulation of apo A-IV mRNA levels, mass, and biogenesis by mono- and polyunsaturated fatty acids (FA) in the human intestinal Caco-2 cell line. In confluent cells incubated with 1 mM oleic (n-9), linoleic (n-6), alpha-linolenic (n-3), or docosahexaenoic (n-3) acids for a long-term period, both apo A-IV protein levels and de novo synthesis were increased. The induction resulted from the up-regulation of apo A-IV mRNA transcripts. In contrast, an inhibitory effect was evident with short-term incubation. FA chain length and degree of unsaturation had little effect altering apo A-IV transcript and biogenesis. These data offer evidence that isolated fatty acids regulate gene expression and the production of apo A-IV in the enterocyte.
Stan
S
S
Departments of Nutrition, Biochemistry and Pathology, Centre de Recherche, Hôpital Sainte-Justine, Université de Montréal, Montréal, Québec H3T 1C5, Canada.
Delvin
E E
EE
Seidman
E
E
Rouleau
T
T
Steinmetz
A
A
Bendayan
M
M
Yotov
W
W
Levy
E
E
D001054:Apolipoproteins A / Q000096:biosynthesis / Q000235:genetics*; D018938:Caco-2 Cells; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology; D019301:Oleic Acid / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017962:alpha-Linolenic Acid / Q000494:pharmacology
D001054:Apolipoproteins A; D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger; C022205:apolipoprotein A-IV; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D019787:Linoleic Acid