title: Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines.
abstract: We have shown that Werner syndrome (WRN) fibroblast cell lines are unusually sensitive to the DNA-damaging agent 4-nitroquinoline 1-oxide (4NQO), though not to gamma radiation or to hydrogen peroxide. The fusion of 4NQO-sensitive WRN and 4NQO-resistant control fibroblast cell lines generated proliferating WRN x control cell hybrids that expressed WRN protein and were 4NQO-resistant. These results establish the recessive nature of 4NQO sensitivity in WRN cell lines and provide a cellular assay for WRN protein function.
authors.affiliation: University of Washington, Department of Pathology, Seattle 98195-7705, USA.
authors.forename: P R
authors.initials: PR
authors.lastname: Prince
authors.forename: C E
authors.initials: CE
authors.lastname: Ogburn
authors.forename: M J
authors.initials: MJ
authors.lastname: Moser
authors.forename: M J
authors.initials: MJ
authors.lastname: Emond
authors.forename: G M
authors.initials: GM
authors.lastname: Martin
authors.forename: R J
authors.initials: RJ
authors.lastname: Monnat
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D000483:Alleles; D015153:Blotting, Western; D002459:Cell Fusion; D002461:Cell Line, Transformed; D002470:Cell Survival; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects*; D005838:Genotype; D006801:Humans; D009153:Mutagens / Q000494:pharmacology*; D010641:Phenotype; D014162:Transfection; D014898:Werner Syndrome / Q000235:genetics*
chemical_list: D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide