Cloning and genetic variability of a HindIII repetitive DNA in Acrossocheilus paradoxus (Cyprinidae).
Thirty clones of a highly repetitive HindIII fragment of DNA from seven populations of Acrossocheilus paradoxus (Cyprinidae) were isolated and sequenced. The fragment represents a tandemly repeated sequence, with a monomeric unit of 270 bp, amounting to 0.08-0.10% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The HindIII satellite DNA family is conserved in three genera of the Cyprinidae. Variation in nucleotide sequences of this repetitive fragment, which is A+T-rich, is distributed both within individuals and among populations. High overall nucleotide divergence (dij = 0.056 +/- 0.001) was detected among clones of the HindIII satellite DNAs of Acrossocheilus paradoxus. Based on the molecular clock hypothesis, the maximum evolutionary rate was estimated to be 5.3 x 10(-7) substitutions per site per year. Lineage sorting may have contributed to the genetic heterogeneity within individuals and populations. Cladistic analyses indicated a closer phylogeographic relationship between populations of the central and south regions in Taiwan.
Wu
W L
WL
Department of Biology, Cheng-Kung University, Tainan, Taiwan.
Wang
J P
JP
Tseng
M C
MC
Chiang
T Y
TY
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000737:chemistry*; D004276:DNA, Satellite / Q000737:chemistry; D015247:Deoxyribonuclease HindIII / Q000378:metabolism*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D004247:DNA; D015247:Deoxyribonuclease HindIII