Optimization of alternate-strand triple helix formation at the 5"-TpA-3" and 5"-ApT-3" junctions.
Alternate-strand triple helix formation was optimized at the two junction steps, the 5"-TpA-3" and 5"-ApT-3" junctions. Footprint experiments, gel retardation assays and thermal denaturation measures on a sequence appropriately designed with two adjacent alternate-strand polypurine tracts points out that the addition of an adenine residue and the removal of one nucleotide should facilitate the crossing strands at the 5"-TpA-3" junction and at the 5"-ApT-3" junction, respectively. These results provide a 'switch code' for the construction of alternate-strand triple helix forming oligonucleotides which open new possibilities for extending the range of applications of antigene strategy.
Brodin
P
P
Laboratoire de Physicochimie et de Pharmacologie des Macromolécules Biologiques, CNRS UMR 8532, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France.
Sun
J S
JS
Mouscadet
J F
JF
Auclair
C
C
D000225:Adenine / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D015195:Drug Design; D008274:Magnesium / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics
D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D004247:DNA; D003850:Deoxyribonuclease I; D008274:Magnesium; D000225:Adenine