[Effect of gamma-aminobutyric acid on Mg2+-ATPase of brain microsomes of the fish Abramis brama L].
The influence of the GABA on the Mg(2+)-ATPase from microsomal fraction of fish brain (Abramis brama L.) was investigation. Preincubation of the microsomes with different concentration of GABA (10(-8)-10(-4) M) stimulated Mg(2+)-ATPase activity. This effect of neuromediator is sensitive to picrotoxine (10(-4) M). It was established that Mg(2+)-ATPase activity stimulated by anions (Cl > Br > F), inhibited by SCN- and not effected by HCO3-. The influence of the anions on the Mg(2+)-ATPase is liable to be inhibited by picrotoxine. It was supposed that anion-sensitive Mg(2+)-ATPase is associated functionally with GABAa-receptor.
Menzikov
S A
SA
I.D. Papanin Institute of Inland Waters Biology, Russian Academy of Sciences, Borok, Yaroslavl Region.
Menzikova
O V
OV
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D017301:Ca(2+) Mg(2+)-ATPase / Q000187:drug effects*; D005399:Fishes; D008861:Microsomes / Q000187:drug effects* / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D005680:gamma-Aminobutyric Acid; D010042:Ouabain; D017301:Ca(2+) Mg(2+)-ATPase