title: Bioassay-directed fractionation of PAH of molecular mass 302 in coal tar-contaminated sediment.
abstract: Bioassay-directed fractionation was used to characterise genotoxic polycyclic aromatic hydrocarbons (PAH) of molecular mass 302 amu in organic solvent extracts of coal tar-contaminated sediment from Sydney Harbour, Nova Scotia. A normal phase HPLC technique was employed to separate PAH-rich solvent extracts into fractions containing PAH of single molecular mass classes. The 302 amu molecular mass fraction was isolated and further separated using reversed phase HPLC; subfractions were collected every 30 s and subjected to bioassay analyses with Salmonella typhimurium strain YG1025 with the addition of oxidative metabolism (4% S9). Compounds eluting in the most active subfractions included naphtho[2,1-alpha]pyrene and naphtho[2,3-alpha]pyrene. The results of this study underscore the significant contribution that molecular mass 302 PAH make to the biological activity of complex environmental mixtures.
authors.affiliation: Department of Chemistry, McMaster University, Hamilton, ON, Canada. chris.marvin@cciw.ca
authors.forename: C H
authors.initials: CH
authors.lastname: Marvin
authors.forename: B E
authors.initials: BE
authors.lastname: McCarry
authors.forename: J A
authors.initials: JA
authors.lastname: Lundrigan
authors.forename: K
authors.initials: K
authors.lastname: Roberts
authors.forename: D W
authors.initials: DW
authors.lastname: Bryant
mesh_terms: D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D003033:Coal Tar / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D019015:Geologic Sediments / Q000032:analysis*; D008970:Molecular Weight; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D009674:Nova Scotia; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012997:Solvents
chemical_list: D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D012997:Solvents; D003033:Coal Tar