High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells.
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.
Duncan
R R
RR
Membrane Biology Group, Biomedical Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9XD, U.K.
Don-Wauchope
A C
AC
Tapechum
S
S
Shipston
M J
MJ
Chow
R H
RH
Estibeiro
P
P
D000313:Adrenal Medulla / Q000166:cytology*; D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000166:cytology / Q000821:virology*; D003470:Culture Media; D004594:Electrophysiology; D005089:Exocytosis; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D012672:Semliki forest virus / Q000235:genetics*
D003470:Culture Media; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins