Analysis of viral glycoproteins by glycosidic digestion inside a polyacrylamide gel.
We adapted the method described by Cleveland et al. (1977); (Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. J. Biol. Chem. 252, 1102-1106) to study the glycosidic residues linked to the viral glycoproteins of two enveloped viruses: Junin virus (JV) and rubella virus (RV). Radioiodinated glycoproteins were obtained from purified virions, isolated from SDS-polyacrylamide gels and then hydrolysed by specific glycosidases inside a second gel. N-linked oligosaccharides, mannose and galactose were found as terminal residues in the JV-G1 glycoprotein. Mannose and N-glycans of complex hybrid type were present on RV glycoproteins.
Grutadauria
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Instituto de Virología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Argentina. slgrtdr@onenet.com.ar
Castilla
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Zapata
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Mersich
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D000818:Animals; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism; D018051:Junin virus / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping / Q000379:methods; D012412:Rubella virus / Q000737:chemistry / Q000302:isolation & purification; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry*
D006023:Glycoproteins; D006027:Glycosides; D007457:Iodine Radioisotopes; D014764:Viral Proteins; D006026:Glycoside Hydrolases