Immune response to rotavirus VP4 expressed in an attenuated strain of Shigella flexneri.
An attenuated strain of Shigella flexneri was utilised to express viral protein (VP) 4 of rotavirus and the immunogenicity of the recombinant constructs was studied in BALB/c mice. VP4 was expressed as a fusion with maltose binding protein (MBP) in both the cytoplasm and periplasm, with a much higher level of expression occurring in the former. While all constructs induced a Shigella-specific response in mice, only the construct expressing MBP-VP4 in the cytoplasm of Shigella stimulated an immune response specific to rotavirus. This study demonstrates that Shigella can be used to deliver rotavirus antigens and induces an immune response directed towards both rotavirus and Shigella.
Loy
A L
AL
Division of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT.
Allison
G
G
Arias
C F
CF
Verma
N K
NK
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000235:genetics; D004405:Dysentery, Bacillary / Q000517:prevention & control; D005260:Female; D058619:Maltose-Binding Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000517:prevention & control; D012763:Shigella flexneri / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated; D014765:Viral Vaccines / Q000276:immunology*
D001428:Bacterial Vaccines; D036022:Capsid Proteins; D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; C060339:VP4 protein, Rotavirus; D014613:Vaccines, Attenuated; D014765:Viral Vaccines