Detection of circulating testicular cancer cells in peripheral blood.
Patients who receive peripheral blood stem cell transplants are at risk of developing cancer recurrence due to the presence of malignant cells in the transplants. We investigated a sensitive method to detect malignant cells in the peripheral blood and peripheral blood stem cells of patients with testicular cancer using nested, reverse transcription-polymerase chain reaction (RT-PCR) to measure alpha-fetoprotein gene expression. Using this technique, a single cancer cell could be detected in 10(6) peripheral blood mononuclear cells. This is the first report of an attempt to detect circulating malignant cells in the peripheral blood of patients with testicular cancer by nested RT-PCR.
Yuasa
T
T
Department of Urology, Shiga University of Medical Science, Otsu, Japan.
Yoshiki
T
T
Tanaka
T
T
Isono
T
T
Okada
Y
Y
D000199:Actins / Q000235:genetics; D000328:Adult; D018236:Carcinoma, Embryonal / Q000097:blood / Q000235:genetics / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D018240:Endodermal Sinus Tumor / Q000097:blood / Q000235:genetics / Q000473:pathology; D015870:Gene Expression; D018237:Germinoma / Q000097:blood / Q000235:genetics / Q000473:pathology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry; D009373:Neoplasms, Germ Cell and Embryonal / Q000097:blood / Q000235:genetics / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013724:Teratoma / Q000097:blood / Q000235:genetics / Q000473:pathology; D013736:Testicular Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000235:genetics
D000199:Actins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000509:alpha-Fetoproteins