title: Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis.
abstract: Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing of pncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-type pncA might possess alternative resistance mechanisms.
authors.affiliation: Laboratory for Pharmaceutical Biotechnology, FFW, University of Ghent, 9000 Ghent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Mestdagh
authors.forename: P A
authors.initials: PA
authors.lastname: Fonteyne
authors.forename: L
authors.initials: L
authors.lastname: Realini
authors.forename: R
authors.initials: R
authors.lastname: Rossau
authors.forename: G
authors.initials: G
authors.lastname: Jannes
authors.forename: W
authors.initials: W
authors.lastname: Mijs
authors.forename: K A
authors.initials: KA
authors.lastname: De Smet
authors.forename: F
authors.initials: F
authors.lastname: Portaels
authors.forename: E
authors.initials: E
authors.lastname: Van den Eeckhout
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000995:Antitubercular Agents / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D015971:Gene Expression Regulation, Enzymologic; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D011718:Pyrazinamide / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D011718:Pyrazinamide; D000581:Amidohydrolases; C022490:pyrazinamide deamidase