A magnetic field-responsive domain in the human HSP70 promoter.
HSP70 gene expression is induced by a wide range of environmental stimuli, including 60-Hz electromagnetic fields. In an earlier report we showed that the induction of HSP70 gene expression by magnetic fields is effected at the level of transcription and is mediated through c-myc protein binding at two nCTCTn sequences at -230 and -160. in the human HSP70 promoter. We report on the identification of a third c-myc binding site (between -158 and -162) that is an important regulator of magnetic field-induced HSP70 expression. We also show that the heat shock element (HSE), lying between -180 and -203, is required for induction of HSP70 gene expression by magnetic fields. The HSE centered at -100 alone is insufficient.
Lin
H
H
Department of Pathology, Columbia University Health Sciences, New York, New York 10032, USA.
Blank
M
M
Goodman
R
R
D001665:Binding Sites; D001667:Binding, Competitive; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D055590:Electromagnetic Phenomena; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D016271:Proto-Oncogene Proteins c-myc