Differential changes in the subcellular distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate and N-methyl-D-aspartate receptors in neonate and adult rat cortex.
We compared the distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors and their individual subunits in synaptosomal and microsomal fractions prepared from 2-day-old (P2) and adult rat cortex. In P2 cortex more [3H]-(S)-fluorowillardiine ([3H]FW) binding to AMPA receptors was in the intracellular microsomal fraction than in the synaptosomal fraction whereas in adult rats the reverse was observed. Immunoblots with GluR1, GluR2/3, GluR4 and pan-AMPA antibodies showed the same profile. In contrast, the majority of [3H]MK-801 binding to the N-methyl-D-aspartate (NMDA) receptor and NR1 subunit immunoreactivity was present in the synaptosomal fraction at both developmental time points. These results suggest a developmental rearrangement of the distribution of AMPA receptors within neurons, a process which is likely to be important in synaptic stabilization and plasticity.
Archibald
K
K
Department of Anatomy, University of Bristol, Medical School, UK.
Molnár
E
E
Henley
J M
JM
D000375:Aging / Q000378:metabolism*; D000409:Alanine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000493:pharmacokinetics; D018690:Excitatory Amino Acid Agonists / Q000493:pharmacokinetics; D005260:Female; D008861:Microsomes / Q000378:metabolism*; D011743:Pyrimidines / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism*; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D011743:Pyrimidines; D018091:Receptors, AMPA; D018094:Receptors, Metabotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; C108822:metabotropic glutamate receptor 2; C102718:metabotropic glutamate receptor 3; C104077:metabotropic glutamate receptor type 1; C095178:5-fluorowillardiine; D016291:Dizocilpine Maleate; D000409:Alanine; C108823:metabotropic glutamate receptor 4