Lack of evidence of conserved lentiviral sequences in pigs with post weaning multisystemic wasting syndrome.
In order to investigate the role of retroviruses in the recently described porcine postweaning multisystemic wasting syndrome (PMWS) serum and leukocytes were screened for reverse transcriptase (RT) activity, and tissues were examined for the presence of conserved lentiviral sequences using degenerate primers in a polymerase chain reaction (PCR). Serum and stimulated leukocytes from the blood and lymph nodes from pigs with PMWS, as well as from control pigs had RT activity that was detected by the sensitive Amp-RT assay. A 257-bp fragment was amplified from DNA from the blood and bone marrow of pigs with PMWS. This fragment was identical in size to conserved lentiviral sequences that were amplified from plasmids containing DNA from several lentiviruses. Cloning and sequencing of the fragment from affected pigs, however, did not reveal homology with the recognized lentiviruses. Together the results of these analyses suggest that the RT activity present in tissues from control and affected pigs is the result of endogenous retrovirus expression, and that a lentivirus is not a primary pathogen in PMWS.
Bratanich
A
A
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Lairmore
M
M
Heneine
W
W
Konoby
C
C
Harding
J
J
West
K
K
Vasquez
G
G
Allan
G
G
Ellis
J
J
D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D016086:Lentivirus / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000821:virology*; D019282:Wasting Syndrome / Q000235:genetics / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral