Suppression of endothelin system in Vero cells latently infected with influenza virus B/Lee/40.
In Vero cells latently infected with influenza virus B/Lee/40 (L/V cells), the endothelin (ET) system was examined as a possible mediator in pathogenesis of latent viral infection by radioimmunoassay (RIA) and quantitative reverse transcription polymerase reaction (RT-PCR). During viral latency of more than two months, ET secretion, preproendothelin-1 (PPET-1) mRNA, and endothelin receptor subtype A (ETAR) mRNA within the cells remained suppressed. Our data indicate that not only the release of ET-1 was downregulated at the transcriptional level but also ETAR mRNA was downregulated rather than upregulated compensatively in L/V cells.
Hayase
Y
Y
Department of Virology, Jichi Medical School, Tochigi, Japan.
Tobita
K
K
D000818:Animals; D002522:Chlorocebus aethiops; D015536:Down-Regulation; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D016232:Endothelins / Q000235:genetics; D009981:Influenza B virus / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells
D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D012333:RNA, Messenger; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin