title: Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum.
abstract: Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and immunoblotting. Among the proteins characterised are the Sec61p complex, the oligosaccharyl transferase (OST) complex, the translocon-associated protein (TRAP) complex, the TRAM and RAMP4 proteins, the signal recognition particle (SRP) and the SRP receptor (SR). Interestingly, the RAMP4 protein, SR and OST complex display more than one oligomeric form.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120, Heidelberg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Dobberstein
mesh_terms: D000818:Animals; D001692:Biological Transport; D002135:Calcium-Binding Proteins / Q000378:metabolism; D037282:Calreticulin; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism*; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism*; D018000:Receptors, Peptide / Q000737:chemistry / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D000069816:SEC Translocation Channels
chemical_list: D002135:Calcium-Binding Proteins; D037282:Calreticulin; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011506:Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D012261:Ribonucleoproteins; D000069816:SEC Translocation Channels; C062477:endoplasmic reticulum glycoprotein p72; C034241:ribophorin; C036318:signal peptide receptor; C066973:signal sequence receptor; D019704:Protein Disulfide-Isomerases