title: Zinc protects against ultraviolet A1-induced DNA damage and apoptosis in cultured human fibroblasts.
abstract: Ultraviolet A1 (UVA1) radiation generates reactive oxygen species and the oxidative stress is known as a mediator of DNA damage and of apoptosis. We exposed cultured human cutaneous fibroblasts to UVA1 radiation (wavelengths in the 340-450-nm range with emission peak at 365 nm) and, using the alkaline unwinding method, we showed an immediate significant increase of DNA strand breaks in exposed cells. Apoptosis was determined by detecting cytoplasmic nucleosomes (enzyme-linked immunosorbent assay method) at different time points in fibroblasts exposed to different irradiation doses. In our conditions, UVA1 radiation induced an early (8 h) and a delayed (18 h) apoptosis. Delayed apoptosis increased in a UVA dose-dependent manner. Zinc is an important metal for DNA protection and has been shown to have inhibitory effects on apoptosis. The addition of zinc (6.5 mg/L) as zinc chloride to the culture medium significantly decreased immediate DNA strand breaks in human skin fibroblasts. Moreover, zinc chloride significantly decreased UVA1-induced early and delayed apoptosis. Thus, these data show for the first time in normal cutaneous cultured cells that UVA1 radiation induces apoptosis. This apoptosis is biphasic and appears higher 18 h after the stress. Zinc supplementation can prevent both immediate DNA strand breakage and early and delayed apoptosis, suggesting that this metal could be of interest for skin cell protection against UVA1 irradiation.
authors.affiliation: Service de Dermatologie, CHU Albert Michallon, Grenoble, France.
authors.forename: M T
authors.initials: MT
authors.lastname: Leccia
authors.forename: M J
authors.initials: MJ
authors.lastname: Richard
authors.forename: A
authors.initials: A
authors.lastname: Favier
authors.forename: J C
authors.initials: JC
authors.lastname: Béani
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002478:Cells, Cultured; D004249:DNA Damage; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D014466:Ultraviolet Rays; D015032:Zinc / Q000494:pharmacology*
chemical_list: D005978:Glutathione; D015032:Zinc