title: Different microcirculatory and interstitial matrix patterns in idiopathic dilated cardiomyopathy and Chagas' disease: a three dimensional confocal microscopy study.
abstract: OBJECTIVE
  To analyse the morphological aspects of the extracellular matrix and microcirculation to clarify whether chronic Chagas' cardiopathy (CCC) is an accurate model to study the pathogenesis of idiopathic dilated cardiomyopathy (IDCM).
  
  
  METHODS
  Thick histological myocardial sections were prepared to analyse collagen, and microcirculation was examined during confocal laser and light microscopy.
  
  
  METHODS
  The specimens were prepared at the pathology service of the Heart Institute of São Paulo, Brazil.
  
  
  METHODS
  Nine control hearts, eight IDCM hearts, and 10 CCC hearts were studied after necropsy.
  
  
  METHODS
  The number of collagen struts per 100x field, the area of fibrosis (%), and the diameters of arterioles and capillaries were measured in each heart to establish outcome.
  
  
  RESULTS
  A smaller number (mean (SD)) of collagen struts was seen in the hearts in the IDCM group (9.1 (4.1)) than in the control (22.4 (3.2)) (p < 0.05) or CCC (15.7 (7.4)) (p > 0.05) groups. Fibrosis was greater in the CCC hearts (13.8 (10.5)%) than in the IDCM hearts (5.9 (6.6)%) (p > 0.05). Major increases in arteriole (65.4 (9.9) microm) and capillary (9.9 (1.7) microm) diameters were seen in the CCC hearts but not in the IDCM hearts (arteriole diameter 40.3 (7.9) microm; capillary diameter 7.9 (1.3) microm).
  
  
  CONCLUSIONS
  Hearts demonstrating CCC and IDCM present different extracellular and microvessel alterations. This suggests that distinct pathogenic mechanisms are responsible for each condition and that CCC is not an effective model to study IDCM.
authors.affiliation: Service of Pathology, Heart Institute of São Paulo University Medical School, Av Dr Eneas C Aguiar, 44-São Paulo, CEP 05403/000, Brazil.
authors.forename: M L
authors.initials: ML
authors.lastname: Higuchi
authors.forename: S
authors.initials: S
authors.lastname: Fukasawa
authors.forename: T
authors.initials: T
authors.lastname: De Brito
authors.forename: L C
authors.initials: LC
authors.lastname: Parzianello
authors.forename: G
authors.initials: G
authors.lastname: Bellotti
authors.forename: J A
authors.initials: JA
authors.lastname: Ramires
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001160:Arterioles / Q000473:pathology; D002196:Capillaries / Q000473:pathology; D002311:Cardiomyopathy, Dilated / Q000378:metabolism / Q000473:pathology*; D002598:Chagas Cardiomyopathy / Q000378:metabolism / Q000473:pathology*; D002648:Child; D003094:Collagen / Q000032:analysis; D003331:Coronary Vessels / Q000473:pathology*; D005109:Extracellular Matrix / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged
chemical_list: D003094:Collagen