Identification of a conserved N-terminal sequence involved in transmembrane signal transduction in EnvZ.
To determine whether N-terminal sequences are involved in the transmembrane signaling mechanism of EnvZ, the nucleotide sequences of envZ genes from several enteric bacteria were determined. Comparative analysis revealed that the amino acid sequence between Pro41 and Glu53 was highly conserved. To further analyze the role of the conserved sequence, envZ of Escherichia coli was subjected to random PCR mutagenesis and mutant alleles that produced a high-osmolarity phenotype, in which ompF was repressed, were isolated. The mutations identified clustered within, as well as adjacent to, the Pro41-to-Glu53 sequence. These findings suggest that the conserved Pro41-to-Glu53 sequence is involved in the signal transduction mechanism of EnvZ.
Waukau
J
J
Department of Biological Sciences, University of Wisconsin, Milwaukee, Wisconsin 53201, USA.
Forst
S
S
D000409:Alanine; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002462:Cell Membrane; D017124:Conserved Sequence; D003470:Culture Media; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D007930:Leucine; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D016296:Mutagenesis; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; C057843:envZ protein, E coli; D010749:Phosphoprotein Phosphatases; D007930:Leucine; D000409:Alanine