Estradiol influences the LH response to met-enkephalin. An immunocytochemical and morphometric study.
In order to test the possible role of estradiol in the response of the LH-adenohypophyseal cells to the administration of met-enkephalin in the albino male rat, an immunocytochemical (peroxidase-antiperoxidase), morphometric (cellular and nuclear areas and numerical density) and radioimmunoassay (LH serum levels at the moment of sacrifice) study was carried out. The intraventricular administration of met-enkephalin (150 micrograms in 25 microliters of distilled water) does not produce any changes in the parameters considered. However, when the animals were pretreated with estradiol (chronically, 15 days), met-enkephalin produced a significant decrease in all the parameters considered.
Rubio
M
M
Departamento de Anatomía e Histología Humanas, Facultad de Medicina, Universidad de Salamanca, Spain.
Carretero
J
J
Sánchez
F
F
Riesco
J M
JM
Vázquez
R
R
Vázquez
R
R
D000818:Animals; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000097:blood / Q000187:drug effects*; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D009292:Narcotic Antagonists; D009270:Naloxone; D004958:Estradiol; D004744:Enkephalin, Methionine; D007986:Luteinizing Hormone