A new potent antigen from Echinococcus granulosus associated with muscles and tegument.
An immunoscreening of a cDNA library derived from the adult stage of the parasitic platyhelminth Echinococcus granulosus has been carried out with sera from infected dogs. The EgA31 clone encodes a fibrous protein which shares some sequence elements with paramyosins. The corresponding gene is present as a single copy in the genome. As revealed by an antibody obtained against a fusion protein produced in bacteria, the polypeptide has a molecular weight of 66 kDa. This polypeptide is present at all developmental stages studied and is a potent antigen during an infection by the adult stage in the dog and during cyst growth in human patients. By immunohistology, it was shown that it is present in the tegument and subtegumental parenchyma of the adult with a main location in the region of the suckers where it rapidly accumulates at the time of the head evagination. It is also present in the germinal layer of the cyst and on the protoscolex.
Fu
Y
Y
C.G.M.C., UMR CNRS 5534, Université Lyon I, Villeurbanne, France.
Martinez
C
C
Chalar
C
C
Craig
P S
PS
Ehrlich
R
R
Petavy
A F
AF
Bosquet
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D004285:Dogs; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary; D004446:Echinococcus / Q000254:growth & development* / Q000276:immunology*; D017238:Genes, Helminth; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009132:Muscles / Q000276:immunology; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D012867:Skin / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C401864:EgA31 protein, Echinococcus granulosus