Use of TUNEL staining to detect apoptotic cells in the lungs of cattle experimentally infected with Pasteurella haemolytica.
Lung sections taken from calves with experimental Pasteurella haemolytica respiratory infection exhibited increased numbers of TUNEL positive cells with time after challenge. This finding suggests that P. haemolytica, or toxins and other components released by the organism, induces apoptosis in bovine leukocytes in vivo. By so doing this might impair host defense and contribute in part to the severe pneumonia that characterizes bovine pasteurellosis.
Leite
F
F
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.
Malazdrewich
C
C
Yoo
H S
HS
Maheswaran
S K
SK
Czuprynski
C J
CJ
D000818:Animals; D017209:Apoptosis; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology*; D020287:In Situ Nick-End Labeling / Q000662:veterinary*; D007962:Leukocytes / Q000473:pathology; D008168:Lung / Q000473:pathology*; D016978:Mannheimia haemolytica; D010326:Pasteurella Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011014:Pneumonia / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D013997:Time Factors
D011933:Reagent Kits, Diagnostic