Effect of adenosine derivatives on in vitro thrombus formation induced by shear stress.
OBJECTIVE
Shear-stress is considered to be the first event of platelet aggregation in vivo and platelet adhesion may be enhanced under pathologic conditions (e.g. arterial occlusion).


METHODS
We wanted to test the effect of adenosine derivatives on platelet aggregation induced by shear-stress. By increasing platelet cAMP adenosine derivatives inhibit platelet activation. This in turn leads to P-selectin (CD62P) exposure, which is known to play a fundamental role in the binding of platelets to leukocytes. This gives rise to thrombus formation.


RESULTS
The levels of cAMP (pmol/mL) prior to and after treating blood with the following compounds were respectively: PGE1 4.67+/-0.29, 9.33+/-0. 58; SP64 5.2+/-0.34, 6.83+/-0.52; 2-Cl-adenosine 5.83+/- 0.58, 7. 45+/-0.55; NECA 7.00+/-2.29, 8.00+/-1.76.


CONCLUSIONS
High shear rate was studied using a filteraggregometer which could be a good test for analyzing what happens under physiologic conditions compared to other systems in which platelet aggregation only occurs after adding aggregating agents (Born aggregometer).
Menegatti
M
M
Angelo Bianchi Bonomi Haemophilia and Thrombosis Center, Institute of Internal Medicine, IRCCS Maggiore Hospital and University of Milan, Italy.
Cristalli
G
G
Gallo
L
L
Mannucci
P M
PM
Pareti
F I
FI
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000242:Cyclic AMP; D006801:Humans; D019007:P-Selectin; D010974:Platelet Aggregation / Q000187:drug effects*; D013314:Stress, Mechanical
D019007:P-Selectin; D000242:Cyclic AMP; D000241:Adenosine