[cDNA cloning of human stem cell factor and its high level expression in E. coli].
The total RNA of HepG2 cell was extracted as the template by ultrocentrifuge method. The full length cDNA (0.8 kb) encoding the human stem cell factor (hSCF) was amplified by RT-PCR method. The cDNA encoding mature hSCF (0.5 kb) was sequenced and was recombined into the expression vector (PBV-220). The expression level of rhSCF in E. coli DH5 alpha was about 15% of the total protein.
Chen
W
W
Institute of Basic Medical Sciences, CAMS, Beijing.
Di
X
X
Li
J
J
Song
F
F
Chen
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D019089:Stem Cell Factor / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D019089:Stem Cell Factor