Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Lentinus edodes.
The glyceraldehyde-3-phosphate dehydrogenase (GPD) gene of Lentinus edodes was isolated from a genomic DNA library and cDNA corresponding to this gene was isolated from a mycelium cDNA library. The L. edodes GPD gene was found to encode a 337-aa protein. By comparison of the cDNA and genomic DNA sequences, the presence of eight introns in the GPD gene was confirmed. The putative amino acid sequence of the L. edodes GPD gene product showed high similarity to those of other basidiomycetes. The results of Southern blot analyses suggested that only one copy of the GPD gene is present in the genome of L. edodes. The promoter region was found to contain a CT-rich stretch, two CAAT boxes and a consensus TATA box. In addition, the transcript of the GPD gene was found to be expressed constitutively and strongly. These results suggest that the promoter of the L. edodes GPD gene may be very useful as a component of transformation vectors.
Hirano
T
T
Iwate Biotechnology Research Center, Japan.
Sato
T
T
Okawa
K
K
Kanda
K
K
Yaegashi
K
K
Enei
H
H
D020413:3' Untranslated Regions / Q000096:biosynthesis / Q000235:genetics; D020121:5' Untranslated Regions / Q000096:biosynthesis / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D020074:Lentinula / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases