title: The synergistic action of ethanol and nerve growth factor in the induction of neuronal nitric oxide synthase.
abstract: Ethanol alone had no effect on neuronal nitric oxide synthase (nNOS) expression in PC12 cells. However, in the presence of nerve growth factor (NGF), nNOS expression was amplified (threefold, P < 0.05), compared to NGF alone. This increase was eliminated with pretreatment of PC12 cells with staurosporine, suggesting that the effects of ethanol on nNOS expression are mediated by a protein kinase C-dependent pathway.
authors.affiliation: Department of Pediatrics, University of California at San Francisco, 94143-0106, USA.
authors.forename: Y T
authors.initials: YT
authors.lastname: Phung
authors.forename: S M
authors.initials: SM
authors.lastname: Black
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D003720:Densitometry / Q000379:methods; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D016716:PC12 Cells / Q000187:drug effects; D011493:Protein Kinase C / Q000187:drug effects; D051381:Rats
chemical_list: D000431:Ethanol; D020932:Nerve Growth Factor; D019001:Nitric Oxide Synthase; D011493:Protein Kinase C