Random amplification of polymorphic DNA fingerprinting of Trypansoma evansi.
The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eight arbitrarily selected 10-base primers. Informative band patterns were obtained for all isolates analysed. Depending upon the T. evansi isolate primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (random amplified polymorphic DNA) profiles.
Basagoudanavar
S H
SH
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar.
Rao
J R
JR
Singh
R K
RK
Butchaiah
G
G
D000818:Animals; D000866:Antelopes / Q000469:parasitology; D002162:Camelus / Q000469:parasitology; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D006736:Horses / Q000469:parasitology; D007194:India; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D016054:DNA, Protozoan