Absence of synergistic effects on micronucleus formation after exposure to electromagnetic fields and asbestos fibers in vitro.
Exposure of human amniotic fluid (AFC) cells to horizontally applied magnetic fields (hMF) of 50 Hz and 1 mT generated in a Helmholtz-coil system leads to a significant increase in micronucleus frequency (MN), without affecting cell proliferation. To investigate whether hMF-exposure has an additive or synergistic effect on the genotoxic capacity of asbestos fibers, MN induction was investigated in hMF pre-exposed cells, treated before or after with asbestos (1 microg/cm2). Neither synergistic nor additive effects on MN induction were observed. The results indicate, that under our experimental conditions, exposure to hMF and treatment with asbestos fibers possess genotoxic capability, but no interactive effects, in AFC cells.
Simkó
M
M
Division of Environmental Physiology, Institute of Cellular Physiology and Biosystems Technology, University of Rostock, Germany. myrtill.simko@biologie.uni-rostock.de
Dopp
E
E
Kriehuber
R
R
D000653:Amniotic Fluid / Q000166:cytology / Q000187:drug effects; D001194:Asbestos / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D015162:Micronucleus Tests; D008940:Mitotic Index / Q000187:drug effects
D002273:Carcinogens; D001194:Asbestos