A newly developed assay for melatonin using cells expressing human mel-1a receptor.
We have developed a radioreceptor binding assay (RRA) method for melatonin using membranes from Chinese hamster ovary cells that can stably express human mel-1a receptors. We measured melatonin levels in plasma samples collected every 4h for 24h using the RRA and radioimmunoassay (RIA) methods, simultaneously. There was a statistically significant correlation between the melatonin levels measured by the two methods, this newly developed method providing a sensitive bioassay. As it is possible to circumvent the cross-reactivity usually occurring in the RIA method, this method may be an important tool for detecting bioactive substances relative to the mel-1a receptor.
Ozeki
Y
Y
Department of Psychiatry, Shiga University of Medical Science, Otsu, Japan.
Yamada
N
N
Aoki
H
H
Yokoyama
T
T
Shirono
H
H
Koga
J
J
Kato
N
N
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008550:Melatonin / Q000097:blood*; D011863:Radioimmunoassay; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D044094:Receptors, Melatonin; D012680:Sensitivity and Specificity; D014162:Transfection / Q000235:genetics
D018076:DNA, Complementary; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin; D008550:Melatonin