title: Noninvasive 3-D ultrasound of atherosclerotic plaques in the Watanabe rabbit.
abstract: We have investigated the ability to quantitate atherosclerosis in the aortic arch of the Watanabe rabbit using noninvasive 3-D ultrasound. Our methodology utilizes postprocessing of videotaped freehand 2-D interrogations to form a compound 3-D data block. Structures may then be segmented on the attributed grey-scale level and volumes measured. Analysis of 3-D reconstructions revealed a low echo structure in the aortic arch of atherosclerotic rabbits, absent in nonatherosclerotic rabbits, at recognized sites of plaque predilection. This structure volume correlated closely with fatty streak volume determined from histology (r = 0.890). During a 30-week study, this structure volume increased in untreated animals, but was blocked by treatment with the antiatherosclerotic agent probucol. Thus, a new 3-D ultrasound methodology has been used noninvasively to detect and quantitate a low echo structure corresponding to fatty streaks in the Watanabe rabbit aortic arch. This new methodology could potentially aid plaque burden quantification in human peripheral arteries.
authors.affiliation: Zeneca Pharmaceuticals, Dept. of Cardiovascular, Musculoskeletal and Metabolism Research, Macclesfield, UK. chris.allott@alderley.zeneca.com
authors.forename: C P
authors.initials: CP
authors.lastname: Allott
authors.forename: C D
authors.initials: CD
authors.lastname: Barry
authors.forename: J
authors.initials: J
authors.lastname: Bramley
authors.forename: N W
authors.initials: NW
authors.lastname: John
authors.forename: P M
authors.initials: PM
authors.lastname: Mellor
authors.forename: D S
authors.initials: DS
authors.lastname: Thomson
authors.forename: C F
authors.initials: CF
authors.lastname: Reilly
mesh_terms: D000465:Algorithms; D000818:Animals; D000924:Anticholesteremic Agents / Q000627:therapeutic use; D001013:Aorta, Thoracic / Q000000981:diagnostic imaging / Q000473:pathology; D001018:Aortic Diseases / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000473:pathology; D001161:Arteriosclerosis / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000473:pathology; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D011341:Probucol / Q000627:therapeutic use; D011817:Rabbits; D013997:Time Factors; D014463:Ultrasonography / Q000379:methods
chemical_list: D000924:Anticholesteremic Agents; D011341:Probucol