title: Invasive pulmonary aspergillosis diagnosed early by polymerase chain reaction assay.
abstract: We compared the usefulness of a polymerase chain reaction (PCR) assay for the early diagnosis of invasive pulmonary aspergillosis with the serodiagnosis of sufficient concentrations of galactomannan using the same serum samples. A patient was treated with prednisolone for the management of hepatitis. Computed tomography (CT) scan of the chest showed the nodular shadow with a cavity containing a clear fungus ball. DNA of Aspergillus spp. from a serum sample was detected and using the same serum sample, both latex agglutination and sandwich enzyme-linked immunosorbent assay (ELISA) of galactomannan were negative. PCR assay provides an early diagnosis of invasive pulmonary aspergillosis compared with ELISA of galactomannan.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine.
authors.forename: S
authors.initials: S
authors.lastname: Kawamura
authors.forename: S
authors.initials: S
authors.lastname: Maesaki
authors.forename: K
authors.initials: K
authors.lastname: Omagari
authors.forename: K
authors.initials: K
authors.lastname: Hashiguchi
authors.forename: K
authors.initials: K
authors.lastname: Tomono
authors.forename: T
authors.initials: T
authors.lastname: Tashiro
authors.forename: S
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000908:Antibodies, Fungal / Q000032:analysis; D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis*; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D007841:Latex Fixation Tests; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000382:microbiology; D008297:Male; D008351:Mannans / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013902:Radiography, Thoracic; D014057:Tomography, X-Ray Computed
chemical_list: D000908:Antibodies, Fungal; D004271:DNA, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose