Chronic effects of somatotropin treatment in vivo and in vitro on lipogenic activity of goat adipose tissue in a glucose-free buffer during acute incubation.
Young castrated male goats (n = 8) were used to investigate the effect of long-term treatment with recombinant methionyl bovine somatotropin in a sustained release vehicle (bST; 100 mg at seven-day intervals in a 147-day experiment) and chronic culture (24 h) of omental adipose tissue in the presence of various hormones on lipogenic responses to catecholamines during acute incubation (2 h) in a sodium acetate supplemented glucose-free buffer. The rate of fatty acid synthesis in freshly-prepared adipose explants was low and did not differ from those cultured in the absence of hormones for 24 h. Hormonal combination of insulin (17 nmol.l(-1)) plus cortisol (138 nmol.l(-1)) or insulin plus recombinant enterokinase linker bST (4.5 nmol.l(-1) increased lipogenesis (P<0.05). Further addition of bST or cortisol decreased lipogenesis significantly (P<0.05) in the controls but not significantly in bST-treated animals. Cultured explants from either control or bST-treated animals showed significant inhibition of lipogenesis by both norepinephrine (10 micromol.l(-1)) and isoprenaline (10 micromol.l(-1)). BST treatment in vivo did not increase the responsiveness of cultured explants to norepinephrine in vitro, however, the responsiveness to isoprenaline(inhibition of lipogenesis) was greater in bST-treated animals than in the controls.
Skarda
J
J
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Prague.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D002417:Cattle; D003470:Culture Media; D046508:Culture Techniques; D005227:Fatty Acids / Q000096:biosynthesis; D005947:Glucose / Q000008:administration & dosage*; D006041:Goats / Q000378:metabolism*; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D009852:Omentum; D009919:Orchiectomy
D002021:Buffers; D003470:Culture Media; D005227:Fatty Acids; D007328:Insulin; D008055:Lipids; D013006:Growth Hormone; D005947:Glucose; D007545:Isoproterenol; D006854:Hydrocortisone; D009638:Norepinephrine