title: Molecular cloning and sequence analysis of a cDNA for factor V activating enzyme.
abstract: The complete amino acid sequence of a factor V activator (VLFVA) is deduced from the nucleotide sequence of a cDNA encoding the enzyme. The cDNA was isolated by PCR screening a venomous gland cDNA library of Central Asian Vipera lebetina snake. The full-length cDNA clone, derived from two overlapping fragments, comprises 1563 basepairs which encode an open reading frame of 259 amino acids. The amino acid sequence of VLFVA (235 amino acids) shows significant homology with snake venom and mammalian serine proteinases. It contains 12 half-cysteines which form, by analogy with other serine proteinases, 6 disulfide bridges. VLFVA has the catalytic triad His43-Asp88-Ser182. The amino terminal amino acid valine is preceded by 24 amino acids: a putative signal peptide of 18, mainly hydrophobic, amino acids and an activating peptide of 6, mainly hydrophilic amino acid residues. This is the first cloned factor V activating enzyme from snake venom.
authors.affiliation: National Institute of Chemical Physics and Biophysics, Tallinn, 12618, Estonia.
authors.forename: E
authors.initials: E
authors.lastname: Siigur
authors.forename: A
authors.initials: A
authors.lastname: Aaspõllu
authors.forename: J
authors.initials: J
authors.lastname: Siigur
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003029:Coagulants / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014757:Viper Venoms / Q000201:enzymology*; D017819:Viperidae / Q000235:genetics*
chemical_list: D003029:Coagulants; D018076:DNA, Complementary; D011506:Proteins; D014757:Viper Venoms; D010447:Peptide Hydrolases; C121422:factor V activating enzyme