Modulation of protein kinase C isoforms by PAF in cerebral cortex.
The effect of platelet activating factor (PAF) on subcellular distribution of protein kinase C isoforms in rat cerebral cortex was investigated. PAF induced an increase in levels of protein kinase C epsilon and gamma in membrane fraction. Results also indicate that PAF induced an increase in protein kinase C delta levels in both cytosolic and membrane fraction. This effect is possibly due to an increase in enzyme synthesis, as indicated by the results obtained from the experiments performed in the presence of cycloheximide and actinomycin. All the effects induced by PAF were time- and dose-dependent, and were mediated through the activation of PAF receptor. These findings indicate that the three isoforms may be involved in signal transduction of PAF in the brain.
Calcerrada
M C
MC
Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense de Madrid, Spain.
Pérez-Alvarez
M J
MJ
Catalán
R E
RE
Martínez
A M
AM
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D007527:Isoenzymes; D010972:Platelet Activating Factor; D011493:Protein Kinase C