title: Using a direct current electrical field to promote spinal-cord regeneration.
abstract: The authors used a direct current electrical field to promote spinal-cord regeneration in a canine model. Thirty-two dogs were randomly divided into four groups. Complete spinal-cord injury was induced, and electrical stimulators were then placed in the animals. Group 1 served as controls; Groups 2 to 4 were experimental groups, with varying stimulator voltages: 0V in Group 1, 12V in Groups 2 and 4, and 6V in Group 3, with the stimulator implanted 6 hr after spinal-cord injury in Group 4. Functional, electrophysiologic and morphometric assessments were carried out 1 to 3 months postoperatively. Results showed that spinal-cord function, cortical somatosensory evoked potentials, number of neurons, sectional area of neurons, and Nissl body density in the experimental groups were much better than those in the control group. In addition, all the indices in Group 2 were better than those in Groups 3 and 4. This indicated that direct current electrical stimulation could effectively promote spinal-cord regeneration and functional recovery in this model. The 12V voltage was safe for the animals. The stimulator was not rejected by the host for a relatively long period of time.
authors.affiliation: Department of Orthopedics, People's Hospital of Hainan Province, Haikou, China.
authors.forename: N J
authors.initials: NJ
authors.lastname: Shen
authors.forename: S C
authors.initials: SC
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D004558:Electric Stimulation / Q000295:instrumentation*; D004574:Electromagnetic Fields; D005500:Follow-Up Studies; D009416:Nerve Regeneration / Q000502:physiology*; D009431:Neural Conduction / Q000502:physiology; D009460:Neurologic Examination; D011897:Random Allocation; D012016:Reference Values; D013119:Spinal Cord Injuries / Q000628:therapy*; D013127:Spinal Nerves / Q000502:physiology