Uptake of oligonucleotides by keratinocytes.
Oligonucleotides (ODNs) conjugated to rhodamin (Rh) and 4-[(N-2-chloroethyl-N-methyl)amino] benzylamine were used to investigate ODNs transport into keratinocytes. Affinity labeling of two proteins, 63 and 35 kDa, and the inhibition of the affinity labeling and ODNs uptake by the cells in the presence of nucleic acids, polyanions and trypsin suggest, that the proteins are involved in transport of nucleic acids in keratinocytes.
Laktionov
P
P
Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Dazard
J E
JE
Piette
J
J
Vives
E
E
Rykova
E
E
Vlassov
V
V
Lebleu
B
B
D001345:Autoradiography; D002460:Cell Line; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D009150:Mustard Compounds / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry
D009150:Mustard Compounds; D009841:Oligonucleotides; D012235:Rhodamines; C025881:4(N)-(2-chloroethyl-N-methylamino)benzylamine