title: The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity.
abstract: ArsA, an anion-stimulated ATPase, consists of two nucleotide binding domains, A1 in the N terminus and A2 in the C terminus of the protein, connected by a linker. The A1 domain contains a high affinity ATP binding site, whereas the A2 domain has low affinity and it requires the allosteric ligand antimonite for binding ATP. ArsA is known to form a UV-activated adduct with [alpha-(32)P]ATP in the linker region. This study shows that on addition of antimonite, much more adduct is formed. Characterization of the nature of the adduct suggests that it is between ArsA and ADP, instead of ATP, indicating that the adduct formation reflects hydrolysis of ATP. The present study also demonstrates that the A1 domain is capable of carrying out unisite catalysis in the absence of antimonite. On addition of antimonite, multisite catalysis involving both A1 and A2 sites occurs, resulting in a 40-fold increase in ATPase activity. Studies with mutant proteins suggest that the A2 site may be second in the sequence of events, so that its role in catalysis is dependent on a functional A1 site. It is also proposed that ArsA goes through an ATP-bound and an ADP-bound conformation, and the linker region, where ADP binds under both unisite and multisite catalytic conditions, may play an important role in the energy transduction process.
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA. bioppk@panther.gsu.edu
authors.forename: P
authors.initials: P
authors.lastname: Kaur
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000838:Anions; D053501:Arsenite Transporting ATPases; D001665:Binding Sites; D002384:Catalysis; D004926:Escherichia coli; D006868:Hydrolysis; D016623:Ion Pumps; D008565:Membrane Proteins / Q000378:metabolism; D009097:Multienzyme Complexes; D013379:Substrate Specificity
chemical_list: D000838:Anions; D016623:Ion Pumps; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases