title: Calcium dependency of arachidonic acid incorporation into cellular phospholipids of different cell types.
abstract: Ca2+ -independent phospholipase A2 (iPLA2) is involved in the incorporation of arachidonic acid (AA) into resting macrophages by the generation of the lysophospholipid acceptor. The role of iPLA2 in AA remodeling in different cells was evaluated by studying the Ca2+ dependency of AA uptake from the medium, the incorporation into cellular phospholipids, and the effect of the iPLA2 inhibitor bromoenol lactone on these events. Uptake and esterification of AA into phospholipids were not affected by Ca2+ depletion in human polymorphonuclear neutrophils and rat fibroblasts. The uptake was Ca2+ independent in chick embryo glial cells, but the incorporation into phospholipids was partially dependent on extracellular Ca2+. Both events were fully dependent on extra and intracellular Ca2+ in human platelets. In human polymorphonuclear neutrophils, the kinetics of incorporation in several isospecies of phospholipids was not affected by the absence of Ca2+ at short times (<30 min). The involvement of iPLA2 in the incorporation of AA from the medium was confirmed by the selective inhibition of this enzyme with bromoenol lactone, which reduced < or =50% of the incorporation of AA into phospholipids of human neutrophils. These data provide evidence that suggests iPLA2 plays a major role in regulating AA turnover in different cell types.
authors.affiliation: Departamento de Química Biológica, Universidad Nacional de Córdoba, Argentina.
authors.forename: J J
authors.initials: JJ
authors.lastname: Daniele
authors.forename: G D
authors.initials: GD
authors.lastname: Fidelio
authors.forename: I D
authors.initials: ID
authors.lastname: Bianco
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
chemical_list: D003470:Culture Media; D010743:Phospholipids; D016718:Arachidonic Acid; D002118:Calcium