Antisense effects of oligonucleotides complementary to the hairpin of the Leishmania mini-exon RNA.
We investigated the binding and the translation inhibitory properties of hexadecamers complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis. This targeted RNA region folds into a hairpin. Large differences were observed in the antisense properties of the different oligomers although their binding to RNA always requires the disruption of the stem region.
Compagno
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INSERM U 386, IFR Pathologies Infectieuses, Université Victor Segalen, Bordeaux, France.
Toulmé
J J
JJ
D000818:Animals; D001483:Base Sequence; D005091:Exons; D007891:Leishmania / Q000187:drug effects* / Q000235:genetics; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D016053:RNA, Protozoan / Q000737:chemistry*
D016376:Oligonucleotides, Antisense; D016053:RNA, Protozoan