Methylobacterium extorquens strain P14, a new methylotrophic bacteria producing poly-beta-hydroxybutyrate (PHB).
Strain P14 of facultative methylotrophic bacteria that synthetisizes poly-beta-hydroxybutyrate has been isolated. The cells are gram-negative motile rods with a polar flagellum. They do not form spores or capsules, but do have a caretenoid pigment. Predominant in the fatty acid composition of the cells is cis-vaccenic acid (cis 18:1: omega 7)--72%. In the phospholipid composition phosphatidylcholine predominates (45%), along with phosphatidylenthanoloamine (27%) and phosphatidylglycerol (17%). The main biquinone is Q-10; other ubiquinones (Q-8, Q-9, Q-11) are present in minor quantities. The cells accomplish the icl-variant of serine pathway. The GC content of DNA (Tm) is 65 mole%. A high level DNA-DNA homology with representatives of the genus Methylobacterium was observed. The strain has been identified as Methylobacterium extorquens strain P14.
Ostafin
M
M
Institute Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region.
Haber
J
J
Doronina
N V
NV
Sokolov
A P
AP
Trotsenko
Y A
YA
D003470:Culture Media / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D005227:Fatty Acids; D006885:Hydroxybutyrates; D000432:Methanol; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates