title: Specific inhibition of TH2-type cytokine production from human peripheral T cells by terfenadine in vitro.
abstract: BACKGROUND
  Cytokine imbalance is thought to be one of the causes for allergic diseases. The effect of anti-allergic drugs on cytokine production from T cells should be examined in a convenient way.
  
  
  OBJECTIVE
  To study the in vitro effect of terfenadine, a prototype non-sedating H1 receptor antagonist, on cytokine production from activated T cells.
  
  
  METHODS
  T cells were cultured in the presence of terfenadine on anti-CD3 mAb and anti-CD26 mAb-coated wells, anti-CD3 mAb and anti-CD28 mAb-coated wells, and anti-CD3 mAb wells with PMA. T-cell proliferation, along with the concentrations of interleukin (IL) -2, interferon (IFN) -gamma, IL-4, and IL-5 were measured.
  
  
  RESULTS
  Terfenadine inhibited T-cell proliferation and IL-4 and IL-5 production under each costimulatory condition tested, whereas it had no effect on IL-2 and IFN-gamma production.
  
  
  CONCLUSIONS
  These results indicate that terfenadine has a specific inhibitory effect on TH2-type cytokine production induced by several ways of costimulatory activation.
authors.affiliation: Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Munakata
authors.forename: Y
authors.initials: Y
authors.lastname: Umezawa
authors.forename: S
authors.initials: S
authors.lastname: Iwata
authors.forename: R P
authors.initials: RP
authors.lastname: Dong
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.forename: C
authors.initials: C
authors.lastname: Morimoto
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008213:Lymphocyte Activation / Q000187:drug effects; D013601:T-Lymphocytes / Q000502:physiology; D016593:Terfenadine / Q000494:pharmacology*; D018418:Th2 Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D016207:Cytokines; D006634:Histamine H1 Antagonists; D016593:Terfenadine