Reactivity of a dual amplified chlamydia immunoassay with different serovars of Chlamydia trachomatis.
A study was undertaken with different serovars (D, E, F, L2, MoPn) of Chlamydia trachomatis to determine the analytical sensitivity of a new dual amplified immunoassay (IDEIA PCE Chlamydia) for detecting chlamydial lipopolysaccharide. IDEIA PCE Chlamydia incorporates a polymer conjugate consisting of multiple copies of antibody and enzyme molecules to provide signal amplification. The test was also assessed with different protein A producing strains of Staphylococcus aureus in order to assess whether the use of a multiple antibody conjugate increased nonspecific binding. The detection limits varied for each serovar with a detection limit of 38 IFU/ml obtained with serovar F and 237 IFU/ml obtained with serovar D. The incorporation of the polymer conjugate resulted in a 2-5 fold increase in analytical sensitivity compared to an earlier version of the test using a conventional conjugate. No increase in cross reactivity with protein A producing strains of S. aureus was obtained. The new dual amplified test format offers potential as a sensitive low-cost screening assay for C. trachomatis infections.
Okadome
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Department of Microbiology, School of Medicine, Fukuoka University, Japan.
Notomi
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Nomura
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Nagayama
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D000940:Antigenic Variation / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D002460:Cell Line; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000302:isolation & purification; D003429:Cross Reactions / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008070:Lipopolysaccharides / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000276:immunology
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D011933:Reagent Kits, Diagnostic