Protective effects of capillarisin on tert-butylhydroperoxide-induced oxidative damage in rat primary hepatocytes.
Capillarisin (Cap), a main constituent of Artemisia capillaris (Compositae), was studied for its antioxidant bioactivity. In the preliminary study, Cap expressed a antioxidant property by its capacity for quenching the free radicals of 1,1-diphenyl-2-picrylhydrazyl (DPPH). This antioxidant bioactivity of Cap was investigated further using a model of t-butylhydroperoxide (t-BHP)-induced cytotoxicity and genotoxicity in rat primary hepatocytes. Results presented here demonstrate that Cap, at concentrations of 0.01-1.00 mg/ml, significantly decreased the leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) and the formation of malondialdehyde (MDA) induced by 30 min treatment of t-BHP (1.5 mM) in primary cultured rat hepatocytes. Cap also attenuated the t-BHP-induced diminution of glutathione (GSH) and high level of DNA repaired synthesis. These results lead to speculation that Cap presents inhibitory effects against t-BHP-caused cytotoxicity and genotoxicity in rat primary hepatocyte cultures at least via two distinct pathways, stabilizing the GSH system and quenching free radicals.
Chu
C Y
CY
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan, Republic of China.
Tseng
T H
TH
Hwang
J M
JM
Chou
F P
FP
Wang
C J
CJ
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury; D002756:Cholagogues and Choleretics / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity; D010647:Phenyl Ethers / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
D000975:Antioxidants; D002756:Cholagogues and Choleretics; D002867:Chromones; D005609:Free Radicals; D009153:Mutagens; D010647:Phenyl Ethers; D008315:Malondialdehyde; C011185:capillarisin; D020122:tert-Butylhydroperoxide; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase