Comparison of dynamic and integrated light-scattering techniques in the study of the interaction of Candida rugosa lipase with DPPC liposomes.
Dynamic light-scattering (DLS) and wide angle integrated light-scattering (WAILS) spectroscopies were evaluated in the study of binding of Candida rugosa lipase (CRL) with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes. The use of cumulants analysis on DLS data allowed for the determination of general lipase-liposome-binding trends. Particle intensity distributions obtained from DLS data by a discrete inversion method revealed the different populations created upon lipase-liposome interactions. Using a discrete inversion technique on WAILS data, not only these populations could be differentiated but also accurate number distributions were obtained in short periods of time. Both DLS and WAILS are excellent tools for the study of lipase binding to lipid vesicles; however, care must be exercised in the analysis of the experimental data whenever particle size distributions are multimodal. The selection of the light scattering technique will depend on the information required.
López-Amaya
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C
Department of Food Science, University of Guelph, Ontario, Canada.
Marangoni
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AG
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002175:Candida / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008049:Lipase / Q000737:chemistry*; D008081:Liposomes; D008956:Models, Chemical; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D055672:Static Electricity; D013696:Temperature
D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D008049:Lipase