Human T-cell lymphotrophic virus type-I tax gene induces secretion of human macrophage inflammatory protein-1alpha.
Human T-cell lymphotropic virus I (HTLV-I) encodes for a 40-kDa protein, Tax, which is important for the immortalization of T cells. Tax has been shown to transactivate several cellular genes. In this study, we show that MIP-1alpha is selectively expressed and secreted in the tax transfected Jurkat cell line upon mitogen stimulation. Expression of MIP-1alpha-R mRNA in these cells suggests an autocrine role for this chemokine in HTLV-I infected T-cells. Induced MIP-1alpha expression and secretion in PMA/PHA stimulated tax transfected cells correlate with the noninduction of MNP-1 transcription factor, which is intimately involved in downmodulating the MIP-1alpha gene.
Sharma
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Department of Biology, University of West Florida, Pensacola, Florida, 32514, USA. vsharma@uwf.edu
May
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CC
D019898:Autocrine Communication; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019742:Chemokines, CC / Q000378:metabolism*; D003001:Cloning, Molecular; D015536:Down-Regulation; D016355:Genes, pX; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019742:Chemokines, CC; C114015:MNP-1 protein, human; D019402:Macrophage Inflammatory Proteins; D008934:Mitogens; D010835:Phytohemagglutinins; D014157:Transcription Factors; D013755:Tetradecanoylphorbol Acetate