N-acetylcysteine in pig liver transplantation from non-heart-beating donors.
Lipid peroxidation due to oxygen free radicals (OFR) seems to play a major role in loss of liver graft viability after warm ischemia, preservation, and transplantation. N-acetylcysteine (NAC) is an antioxidant that has a direct effect on OFR, and is also a glutathione precursor, another antioxidant. This study was designed to evaluate the efficacy of NAC in preventing ischemia-reperfusion damage of liver grafts harvested from non-heart-beating donors. Liver transplantation was performed on pigs divided into five groups: group 1 (control group; n=5) received livers from heart-beating donors; livers were subjected to 30 min of warm ischemia in groups 2 (n=3, no NAC) and group 3 (n=3; NAC treatment); warm ischemia time lasted 60 min in groups 4 (n=4; no NAC) and 5 (n=5; NAC treatment). Studied parameters included graft survival for more than 3 days, aspartate aminotransferase plasma levels, liver histology, and hepatic total glutathione concentrations. Graft survival was 100% in groups 1, 2, and 3, 0% in group 4, and 20% in group 5. NAC treatment did not influence initial mean aspartate aminotransferase release which was greater in warm ischemic livers than in controls. NAC treatment had no effect on liver hepatic total glutathione after reperfusion of animals receiving warm ischemic grants. Finally, no effect on liver histology was observed with NAC treatment. Our study suggests that in liver transplantation from non-heart-beating donors, NAC has no effect in both graft viability and lipid peroxidation. The role of OFR in primary dysfunction of transplanted warm ischemic livers remains controversial.
Manika
A
A
Research Center, Sainte-Justine Hospital, Montreal, Quebec, Canada.
Trinh
T
T
Lagacé
G
G
Dugas
M A
MA
Proulx
F
F
Lepage
G
G
Champagne
J
J
Lavoie
J C
JC
Cousineau
J
J
Russo
P
P
Chartrand
C
C
Yandza
T
T
D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000032:analysis; D006085:Graft Survival / Q000187:drug effects; D007275:Injections, Intravenous; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry / Q000473:pathology; D016031:Liver Transplantation / Q000473:pathology; D013552:Swine; D014019:Tissue Donors; D009927:Tissue and Organ Procurement / Q000379:methods
D001219:Aspartate Aminotransferases; D005978:Glutathione; D000111:Acetylcysteine