title: Characterisation of the PsbX protein from Photosystem II and light regulation of its gene expression in higher plants.
abstract: The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles but absent from light-harvesting complexes or PSII reaction centres. PsbX is, however, localised to the near vicinity of the reaction centre because it can be cross-linked to cytochrome b559, which is known to be associated with the D1/D2 dimer. We also show that the expression of this protein is tightly regulated by light, since neither protein nor mRNA is found in dark-grown plants.
authors.affiliation: Department of Biochemistry. Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
authors.forename: L X
authors.initials: LX
authors.lastname: Shi
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Marchant
authors.forename: C
authors.initials: C
authors.lastname: Robinson
authors.forename: W P
authors.initials: WP
authors.lastname: Schröder
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D002736:Chloroplasts / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics* / Q000378:metabolism; D045332:Photosystem II Protein Complex; D010944:Plants / Q000235:genetics / Q000528:radiation effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D018724:Spinacia oleracea / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D012333:RNA, Messenger; C113691:photosystem II, PSII-X subunit