Anti-T antibodies and peanut-agglutinin-binding glycoproteins in sera of patients with gastric cancer.
Agglutinating antibodies to neuraminidase-treated red blood cells (anti-T agglutinins) are known to be reduced in patients with gastric cancer. The antigenic determinant of anti-T agglutinin is known to have a disaccharide structure [Gal(beta1-3)GalNAc], the same specificity as peanut agglutinin (PNA). We examined sera of 27 patients with gastric cancer and 30 controls for anti-T agglutinins, anti-T antibodies and PNA-binding glycoproteins. Anti-T agglutinins were titrated by a microtiter hemagglutination method. Levels of anti-T antibodies were determined by enzyme immunoassay using synthetic glycoconjugate [Gal(beta1-3)GalNAc O-alpha-linked to human serum albumin] as an antigen. Levels of PNA-binding glycoproteins in sera were measured by sandwich enzyme-linked lectin assay using wheat germ agglutinin and peroxidase-conjugated PNA. Titers of anti-T agglutinins were significantly lower in patients with gastric cancer than in controls (P = 0.041). Levels of anti-T antibodies were not significantly different in patients with gastric cancer and controls; however, decreased levels of anti-T antibodies were more frequent in patients with gastric cancer than in controls (P = 0. 001). Levels of PNA-binding glycoproteins were significantly higher in sera of patients with gastric cancer than in controls (P = 0.001). The levels of anti-T antibodies inversely correlated with the levels of PNA-binding glycoproteins in sera of patients with gastric cancer (r = -0.44, P = 0.021). These results suggest that the decrease in anti-T antibodies in sera of patients with gastric cancer might be due to immune complex formation between circulating PNA-binding glycoproteins and anti-T antibodies.
Hwang
I R
IR
Department of Internal Medicine, Ajou University School of Medicine, Suwon, Kyung-Ki-Do, 442-749, Korea.
Nahm
D H
DH
Cho
S N
SN
Longenecker
B M
BM
Rao Koganty
R
R
Park
I S
IS
D000328:Adult; D000368:Aged; D000373:Agglutinins / Q000378:metabolism; D000912:Antibodies, Neoplasm / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019887:Peanut Agglutinin / Q000378:metabolism*; D037121:Plant Lectins; D011485:Protein Binding; D013274:Stomach Neoplasms / Q000097:blood* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014908:Triticum
D000373:Agglutinins; D000912:Antibodies, Neoplasm; D006023:Glycoproteins; D019887:Peanut Agglutinin; D037121:Plant Lectins