[Changes of PDGF-alpha and beta receptor gene expression in hypoxic rat pulmonary vessels].
OBJECTIVE
To reveal the role of platelet-derived growth factor-alpha and beta receptor in hypoxic pulmonary vascular remodeling. We investigated the changes of PDGF-alpha and beta receptor gene expression under hypoxic condition.


METHODS
Hypoxic pulmonary hypertension rats model was reproduced by normobaric hypoxia. Changes of pulmonary PDGF-alpha and beta mRNA were examined by in situ hybridization.


RESULTS
The PDGF-alpha receptor mRNA of the hypoxia group (3 days) declined to 86% compared with that of the control group, and shooting up to 217% of control at day 7, then sustained at 224% until day 14. (all P < 0.001). PDGF-beta receptor mRNA markedly increased at day 3 after hypoxia and maintained this increasing tendency consistently. Its top was 164% at day 14 (P < 0.001). The growth inhibitor, heparin had alleviated the increases of both rat pulmonary arterial pressure and PDGF receptor gene expression.


CONCLUSIONS
Hypoxia led to the increase of PDGF-alpha and beta receptor gene expression in pulmonary vascular endothelial and smooth muscle cells, and might be involved in hypoxic pulmonary vascular remodeling.
Huang
Q
Q
Institude of Basic Medical Sciences, Beijing.
Sun
R
R
D000818:Animals; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000378:metabolism*; D000860:Hypoxia / Q000150:complications; D008168:Lung / Q000378:metabolism; D008297:Male; D011651:Pulmonary Artery / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta; D017479:Receptors, Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta; D017479:Receptors, Platelet-Derived Growth Factor