Generalised H-point standard addition method for the isolation of the analyte signal from the sample signal when coelution of unknown compounds occurs in liquid chromatography.
The generalised H-point standard addition method (GHPSAM) is proposed for isolating the analytical signal of an analyte from the signal of an unknown sample. Samples containing two and three coeluting compounds have been analysed. The accuracy of the predictions depends on the shape of the analyte and interferent spectra but not on the degree of chromatographic overlap. This methodology involves the location of linear intervals for the unknown interference spectrum from the spectrum of the sample. Once the linear interval has been found the selection of three wavelengths within the interval will allow the cancellation of the signal of the unknown interferent. The method has been applied to the determination of diuretics, amphetamines and phenols in water.
Campíns Falcó
P
P
Departament de Química Analtica, Facultad de Química, Universidad de Valencia, Burjassot, Spain. pilar.campins@uv.es
Bosch Reig
F
F
Blasco Gómez
F
F
Herráez Hernández
R
R
Molins Legua
C
C
D000662:Amphetamines / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004232:Diuretics / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000662:Amphetamines; D004232:Diuretics; D010636:Phenols