Chromosome painting of Y chromosomes and isolation of a Y chromosome-specific repetitive sequence in the dioecious plant Rumex acetosa.
The dioecious plant Rumex acetosa has a multiple sex chromosome system: XX in female and XY(1)Y(2) in male. Both types of Y chromosome were isolated from chromosome spreads of males by manual microdissection, and their chromosomal DNA was amplified using degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). When the biotin-labeled DOP-PCR product was hybridized with competitor DNA in situ, the fluorescent signal painted the Y chromosomes. A library of Y chromosome DNA was constructed from the DOP-PCR product and screened for DNA sequences specific to the Y chromosome. One Y chromosome-specific DNA sequence was identified and designated RAYSI (R. acetosa Y chromosome-specific sequence I). RAYSI is a tandemly arranged repetitive DNA sequence that maps to the 4',6-diamidino-2-phenylindole bands of both Y chromosomes.
Shibata
F
F
Institute of Biology, Faculty of Science, Ehime University, Matsuyama 790-8577, Japan.
Hizume
M
M
Kuroki
Y
Y
D001483:Base Sequence; D020223:Chromosome Painting; D017931:DNA Primers; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014998:Y Chromosome
D017931:DNA Primers