Formation of triple helices at irregular poly (R.Y) sites located in critical positions in the human bcr promoter.
Two polypurine sequences interrupted respectively by one and two adjacent pyrimidines have been identified in the promoter of the human bcr gene. Although these targets are irregular they are recognised and tightly bound by AG and GT motif triplex-forming oligonucleotides. Thermodynamic and kinetic data are presented.
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Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine.
Del Terra
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Scaggiante
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Manzini
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Quadrifoglio
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D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D015513:Oncogene Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D013816:Thermodynamics
D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D004247:DNA; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr