title: Micromechanical effects in the cochlea of tetracaine.
abstract: Local anesthetics applied in the tympanic cavity have earlier been shown to affect the gross receptor potentials in reducing the cochlear microphonics and increasing the positive summating potential. To study the effects of this drug on the mechanical responses in the cochlea, vibrations were measured using laser heterodyne interferometry in an isolated in vitro temporal bone preparation from the guinea pig. Measurements were made at a set of frequencies in the fourth cochlear turn from the Hensen's cells and the outer hair cells in response to sound applied to the ear. The tuning curves of the fundamental and the second harmonic components of the vibratory responses were plotted. When 2 mM tetracaine was applied, the high frequency slope of the second harmonic curve shifted down in frequency, this caused the frequency of the maximum of second harmonic tuning to shift down. These changes were reversible when tetracaine was washed out. Observations were also made in the temporal bone preparation in vitro with a confocal microscope. Fluorescent probes were used to label various structures in the organ of Corti. Optical sections were obtained by tilting the organ permitting a view from the side like a radial section through the organ. Images were acquired before, during and after application of tetracaine and were later analyzed with a computer program. Simultaneously, cochlear microphonics and the summating potential were obtained to monitor the electrical response of the preparation. Although the cochlear microphonics and summating potential decreased when 2 mM tetracaine was applied, structural changes were not measurable in the organ of Corti. The decrease was reversible when tetracaine was washed out. It is concluded that tetracaine affected the high frequency part of the non-linear second harmonic component, possibly by lowering the stiffness of the stereocilia bundle or the body of the outer hair cells.
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. wanje.jager@fyfa.ki.se
authors.forename: W
authors.initials: W
authors.lastname: Jäger
authors.forename: S M
authors.initials: SM
authors.lastname: Khanna
authors.forename: B
authors.initials: B
authors.lastname: Flock
authors.forename: A
authors.initials: A
authors.lastname: Flock
mesh_terms: D000161:Acoustic Stimulation; D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D003051:Cochlea / Q000166:cytology / Q000187:drug effects* / Q000503:physiopathology*; D003055:Cochlear Microphonic Potentials / Q000187:drug effects; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007368:Interferometry; D007834:Lasers; D018613:Microscopy, Confocal; D009925:Organ of Corti / Q000187:drug effects / Q000502:physiology; D013701:Temporal Bone / Q000502:physiology; D013748:Tetracaine / Q000494:pharmacology*; D014732:Vibration
chemical_list: D000779:Anesthetics, Local; D013748:Tetracaine