title: Effect of lysophosphatidic acid on the ovum transport in mouse oviducts.
abstract: The effects of lysophosphatidic acid (LPA) on ovum transport in mouse oviducts were studied. When excised oviducts were incubated at 37 degrees C under 5% CO2 in humidified air for 24 hours, addition of LPA at 10 microM to the medium significantly accelerated the rate of ovum transport, and 1 microM LPA slightly increased the ovum transport rate. These increases were not inhibited by 10 microM indomethacin, a cyclooxygense inhibitor, but were suppressed by 260 ng/ml of pertussis toxin or 10 microM verapamil, a voltage-sensitive calcium channel blocker. These data suggested that LPA stimulates mouse ovum transport by contracting oviductual smooth muscle via a voltage-sensitive calcium channel mediated by a pertussis toxin-sensitive G-protein-linked receptor.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kunikata
authors.forename: S
authors.initials: S
authors.lastname: Yamano
authors.forename: A
authors.initials: A
authors.lastname: Tokumura
authors.forename: T
authors.initials: T
authors.lastname: Aono
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D005187:Fallopian Tubes / Q000187:drug effects*; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008246:Lysophospholipids / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010066:Ovum Transport / Q000187:drug effects*; D037342:Pertussis Toxin; D014700:Verapamil / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002121:Calcium Channel Blockers; D008246:Lysophospholipids; D010566:Virulence Factors, Bordetella; D014700:Verapamil; D037342:Pertussis Toxin; D007213:Indomethacin