Expression of human herpesvirus-8 (HHV-8) encoded pathogenic genes in Kaposi's sarcoma (KS) primary lesions.
Transcription of six different HHV-8 specific mRNAs was examined in early- and late-stage KS primary lesions. Expression of the latency-associated T0.7 mRNA and of VP23 mRNA which is a specific marker of lytic/productive infection suggested that HHV-8 is secondarily recruited into the KS lesions by productively infected monocytes, macrophages. From these cells HHV-8 is transmitted to the KS spindle cells, which are latently infected. v-BCL-2, v-MCP-1 and v-IL-6 were not expressed in latently infected KS spindle cells, therefore the impact of these factors in KS pathogenesis appears to be low. By contrast, v-Cyclin D was highly expressed in almost all latently infected spindle cells and may therefore be an important factor triggering progression of late-stage KS lesions.
Ascherl
G
G
Max Planck Institute for Biochemistry, Department of Virology, Martinsried, Germany.
Hohenadl
C
C
Monini
P
P
Zietz
C
C
Browning
P J
PJ
Ensoli
B
B
Stürzl
M
M
D000368:Aged; D018925:Chemokines / Q000378:metabolism; D056741:Cyclin D; D016213:Cyclins / Q000378:metabolism; D015870:Gene Expression; D005814:Genes, Viral; D015658:HIV Infections / Q000150:complications; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009000:Monocytes / Q000378:metabolism / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012514:Sarcoma, Kaposi / Q000150:complications / Q000378:metabolism / Q000821:virology*; D012878:Skin Neoplasms / Q000150:complications / Q000378:metabolism / Q000821:virology*; D014774:Virulence / Q000235:genetics
D018925:Chemokines; D056741:Cyclin D; D016213:Cyclins; D012333:RNA, Messenger; D012367:RNA, Viral