title: Localization of the N-methyl-D-aspartate R1 receptor in the pituitary gland of immature rainbow trout (Oncorhynchus mykiss).
abstract: A study was conducted on the selective immunostaining of pituitary cells and pars nervosa of immature rainbow trout by antibody raised against the R1 subunit of the rat ionotropic N-methyl-D-aspartate (NMDA) receptor. In the pars distalis, the mammotrophs of the rostral pars distalis exhibited the most marked imunoreactivity, with the somatotrophs of the proximal pars distalis showing a consistently lower degree of immunoreactivity. No imunoreactivity was associated with the corticotrophs, thyrotrophs or gonadotrophs. In the pars intermedia, the melanotrophs showed no evidence of immunoreactivity, whereas the putative somatomammotrophs exhibited a wide inter-animal range of immunoreactivity. Some imunostaining was also evident in the anterior and posterior part of the pars nervosa, at the interface of the pars distalis or pars intermedia with the pars nervosa.
authors.affiliation: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: A C
authors.initials: AC
authors.lastname: Holloway
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: J F
authors.initials: JF
authors.lastname: Leatherland
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D007150:Immunohistochemistry; D017686:Oncorhynchus mykiss; D010902:Pituitary Gland / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000187:drug effects
chemical_list: D004958:Estradiol; D016194:Receptors, N-Methyl-D-Aspartate