Frequent expression of mucin core protein MUC1 in non-neoplastic gallbladder mucosa from patients with pancreaticobiliary maljunction.
BACKGROUND
Gallbladder carcinoma is known to develop frequently in patients with pancreaticobiliary maljunction, though the causal relationship remains speculative.


METHODS
Histopathologic changes, expression of mucin core protein MUC1 and MUC2, and cell proliferative activities in the gallbladder mucosa from 27 patients with panceaticobiliary maljunction and 21 control gallbladders were examined. Three cases of pancreaticobiliary maljunction were associated with gallbladder carcinoma.


RESULTS
The lining epithelia of the non-neoplastic gallbladder mucosa of pancreaticobiliary maljunction showed frequently papillary hyperplasia and higher proliferative activities, when compared to the control. In 3 cases with carcinoma, MUC1 was expressed on the luminal border and in the cytoplasm of carcinoma cells, particularly in de-differentiated and invasive areas. MUC1 was variably expressed on the luminal surface of the lining epithelia of non-neoplastic gallbladder mucosa in babies, children, youths and adults with pancreaticobiliary maljunction. However, such expression was focally seen in 2 of the 21 control cases (p<0.01). MUC2 was scattered in the hyperplastic and carcinomatous epithelial cells appearing as goblet cells in pancreaticobiliary maljunction and control groups.


CONCLUSIONS
This study suggests that persistent MUC1 expression and increased cell proliferative activities of non-neoplastic gallbladder epithelium of the patients with pancreaticobiliary maljunction after birth reflect an altered phenotype of epithelial cells and these abnormalities may be related to carcinogenesis in such patients.
Yamato
T
T
Department of Pathology (II), Kanazawa University School of Medicine, Fukui, Japan.
Kurumaya
H
H
Ohama
K
K
Yamamichi
N
N
Watanabe
Y
Y
Harada
K
K
Sasaki
M
M
Nakanuma
Y
Y
D000328:Adult; D000368:Aged; D015415:Biomarkers; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D003135:Common Bile Duct / Q000002:abnormalities*; D003137:Common Bile Duct Diseases / Q000378:metabolism / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D005704:Gallbladder / Q000378:metabolism* / Q000473:pathology; D005706:Gallbladder Neoplasms / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000096:biosynthesis*; D055262:Mucin-2; D009077:Mucins / Q000096:biosynthesis; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010182:Pancreatic Diseases / Q000378:metabolism* / Q000473:pathology; D010183:Pancreatic Ducts / Q000002:abnormalities*; D010446:Peptide Fragments / Q000096:biosynthesis*
D015415:Biomarkers; C116055:MUC1 tandem repeat peptide; C524625:MUC2 protein, human; D018396:Mucin-1; D055262:Mucin-2; D009077:Mucins; D009363:Neoplasm Proteins; D010446:Peptide Fragments