title: New techniques for the rapid characterization of oligonucleotides by mass spectrometry.
abstract: Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors < 0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.
authors.affiliation: Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112-5820, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: McCloskey
authors.forename: A B
authors.initials: AB
authors.lastname: Whitehill
authors.forename: J
authors.initials: J
authors.lastname: Rozenski
authors.forename: F
authors.initials: F
authors.lastname: Qiu
authors.forename: P F
authors.initials: PF
authors.lastname: Crain
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis*
chemical_list: D009841:Oligonucleotides