title: Isolation and expression analysis of two stress-responsive sucrose-synthase genes from the resurrection plant Craterostigma plantagineum (Hochst.).
abstract: The enzyme sucrose synthase (UDP-glucose: D-fructose 2alpha-glucosyltransferase, EC 2.4.1.13) is a key enzyme in carbohydrate metabolism, catalyzing the reversible conversion of sucrose uridine-diphosphate into fructose and UDP-glucose. We report the molecular characterization of two classes of cDNA and genomic clones encoding sucrose synthase from Craterostigma plantagineum Hochst., a resurrection plant in which the turnover of sucrose is considered to have an important role in the unique phenomenon of surviving desiccation. Sucrose-synthase transcript and protein levels are modulated by dehydration and rehydration. In-situ hybridization revealed that transcripts preferentially accumulate in phloem tissues. Promoter analysis underlined a role for class-I sucrose-synthase genes in dehydration stress and in response to cis-abscisic acid. A DNA sequence motif common to class-I sucrose-synthase and sucrose-phosphate-synthase genes was discovered.
authors.affiliation: Max Planck Institut für Züchtungsforschung, Carl von Linné Weg 10, D-50829 Köln, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kleines
authors.forename: R C
authors.initials: RC
authors.lastname: Elster
authors.forename: M J
authors.initials: MJ
authors.lastname: Rodrigo
authors.forename: A S
authors.initials: AS
authors.lastname: Blervacq
authors.forename: F
authors.initials: F
authors.lastname: Salamini
authors.forename: D
authors.initials: D
authors.lastname: Bartels
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant; D005964:Glucosyltransferases / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana
chemical_list: D018744:DNA, Plant; D012333:RNA, Messenger; D005964:Glucosyltransferases; C022699:sucrose synthase