Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2.
Two types of strains of serotype I of infectious bursal disease virus (IBDV) have been described, on the basis of their ability (IBDV-TC) or inability (IBDV-BU) to infect chicken embryonic cells in culture. However, both types infect B lymphocytes in the bursa of Fabricius of young chickens. To determine the molecular basis for tissue culture infectivity, virus recombinants with chimeric segments A were constructed from IBDV-TC and IBDV-BU by reverse genetics. The region responsible for the different phenotypes was located in VP2. Site-directed mutagenesis identified single amino acids that are responsible for the restriction in infectivity. However, the appropriate amino acid exchanges are strain-specific.
Mundt
Egbert
E
Institute of Molecular Biology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, D-17498 Insel Riems, Germany1.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002642:Chick Embryo; D046508:Culture Techniques; D004279:DNA, Viral; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis; D018075:RNA, Complementary; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics*
D004279:DNA, Viral; D018075:RNA, Complementary; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins