title: [DNA typing methods of HLA].
abstract: Human leukocyte antigens (HLA) are a group of cell membrane proteins encoded by the HLA gene complex on the short arm of the chromosome 6, with a high degrees of polymorphism, and a total of about 650 alleles have been identified at HLA-A, B, C and DR loci up to now, which regulate the immune response by self/not-self discrimination. A number of DNA-based typing methods using specific primers, specific probes or restriction enzymes have been developed. In HLA allele typing, a few methods which are simple in handling and able to determine the alleles automatically, have been available.
authors.affiliation: Japanese Red Cross Central Blood Center.
authors.forename: K
authors.initials: K
authors.lastname: Kashiwase
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA