title: Alteration of cytoskeletal structure, integrin distribution, and migratory activity by phagocytic challenge in cells from an ocular tissue--the trabecular meshwork.
abstract: The trabecular meshwork is a specialized tissue in the anterior chamber of the eye that regulates the aqueous humor outflow and controls the intraocular pressure. Cells in the trabecular meshwork are believed to be essential for maintenance of the outflow system, and their malfunctioning may lead to elevation of intraocular pressure and development of glaucoma. These cells are avid phagocytes. Using an in vitro tissue culture system, we have previously shown that bovine trabecular meshwork cells exhibited a short-term loss of cell-matrix adhesiveness after exposure to latex microspheres. The current study showed that 4 h after phagocytosis, the cytoskeletal structure in trabecular meshwork cells was disrupted, the formation of focal contact formation was limited, and the cellular migratory activity was increased. These in vitro responses paralleled those that occur in vivo. By 24 h, all the changes demonstrated returned to normal. Our data suggest that the short-term loss in cell-matrix cohesiveness observed after phagocytic challenge may be related to the reorganization of cytoskeletal structures and the decline of focal contact formation. The altered cell migration may also be interlinked.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago College of Medicine, 60612, USA.
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: B Y
authors.initials: BY
authors.lastname: Yue
mesh_terms: D000818:Animals; D002417:Cattle; D002465:Cell Movement; D003599:Cytoskeleton / Q000648:ultrastructure*; D010587:Phagocytosis; D017469:Receptors, Fibronectin / Q000378:metabolism*; D019030:Receptors, Vitronectin / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D017469:Receptors, Fibronectin; D019030:Receptors, Vitronectin