MRI measurement of cell volume fraction in the perfused rat hippocampal slice.
T(1)-weighted NMR imaging of the isolated perfused rat hippo-campal slice was used to estimate cell volume fraction. Eight brain slices were studied in artificial cerebrospinal fluid (aCSF) using a 600 MHz narrow bore spectrometer and a home built perfusion chamber. Cell volume fraction was calculated as 1 - f(ECS), where f(ECS) is the distribution volume of gadodiamide in the slice. This was determined by measuring the T(1) of the slice before and after perfusion with gadodiamde. A mean cell volume fraction of 0.66 +/- 0. 04 was estimated. The addition of 60 mM mannitol to three of the brain slices produced a 26% decrease in the cell volume fraction. The technique affords a simple means of estimating cell volume fraction and can be extended to produce images reflecting cell density. Magn Reson Med 42:603-607, 1999.
Buckley
D L
DL
Center for Structural Biology and the University of Florida Brain Institute, University of Florida, Gainesville, Florida, USA. buckley@ufbi.ufl.edu
Bui
J D
JD
Phillips
M I
MI
Blackband
S J
SJ
D000818:Animals; D003287:Contrast Media; D019786:Gadolinium DTPA; D006624:Hippocampus / Q000033:anatomy & histology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley
D003287:Contrast Media; D008353:Mannitol; C064925:gadodiamide; D019786:Gadolinium DTPA