The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule.
Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC-->M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8(+) T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.
Furchner
M
M
Children's Research Institute, Children's Hospital, Inc., Department of Pediatrics, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio, USA.
Erickson
A L
AL
Allen
T
T
Watkins
D I
DI
Sette
A
A
Johnson
P R
PR
Walker
C M
CM
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics* / Q000276:immunology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins