Comparison of a microtitration plate ELISA with a standard cultural procedure for the detection of Salmonella spp. in chicken.
A rapid antibody-capture enzyme-linked immunosorbent assay (ELISA) detecting a wide range of Salmonella serotypes and employing only one culture stage was used to analyze the giblets and body cavity rinsings from frozen chickens. The results from the ELISA were compared with those obtained using a standard cultural procedure in current use in two laboratories, Norwich (N) and Ipswich (I), of the Public Health Laboratory Service (PHLS) in the UK. ELISAs were carried out on the same samples at each of two PHLS laboratories and at the Institute of Food Research with good agreement (94% and 90%). When compared with the cultural method there was 80% and 70% agreement with the ELISA with the PHLS(N) and PHLS(I) samples. The ELISA appeared to have a false-positive rate of 17% (samples from PHLS(N)) but on reculture of the "negative" samples this rate fell to 7%. The false-negative rate for the ELISA was 26% (samples from PHLS(N)) which appeared to be due to insufficient growth of the Salmonella spp. in the single cultural step employed in the ELISA rather than lack of recognition by the antibodies. The problem of false negatives with the cultural method is also discussed. These results are comparable to previously published studies relating immunoassays and the conventional procedure for Salmonella detection when analyzing similar samples. Suggestions are made as to how further increases in ELISA efficiency might be brought about.
Wyatt
G M
GM
Department of Food Molecular Biochemistry, Instiute of Food Research, Norwich, UK.
Lee
H A
HA
Dionysiou
S
S
Morgan
M R
MR
Stokely
D J
DJ
Al-Hajji
A H
AH
Richards
J
J
Sillis
A J
AJ
Jones
P H
PH
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005188:False Negative Reactions; D005511:Food Handling; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D011237:Predictive Value of Tests; D012475:Salmonella / Q000254:growth & development* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D003470:Culture Media