title: The role of interleukins 1, 6 and 8 as lymphocyte attractants in the photodermatoses polymorphic light eruption and chronic actinic dermatitis.
abstract: The two photodermatoses, polymorphic light eruption (PLE) and chronic actinic dermatitis (CAD), are characterized by lymphocyte-rich inflammatory infiltrates, the pathogeneses of which are not fully understood. We have therefore studied suction blister fluid (SBF) samples from patients with these conditions before and at two time points after the induction of experimental lesions by means of a solar simulator; this SBF was then tested for the presence of selected cytokines known to induce peripheral blood lymphocyte (PBL) migration in vitro. A specific EL-4 NOB-1 bioassay was used to detect interleukin (IL)-1 activity, which has already been noted in normal skin and this was found in pre-irradiation control samples as well as 1-3 h and 24 h post-irradiation in both patient groups, but at levels not significantly different from those of controls. Use of a B9 cell proliferation assay showed no detectable IL-6-like activity pre-irradiation, but there was substantial activity in samples at both post-irradiation time points in both patient groups. Further, in other experiments, retained SBF samples were tested in an in vitro PBL migration assay in the presence and absence of neutralizing antibodies against IL-1 alpha, IL-1 beta, IL-6 and IL-8; considerable PBL attractant activity was noted in the pre-irradiation SBF from both patient groups; a finding consistent with previous reports of such activity in samples from normal skin, and at least in CAD patients, a proportion of this activity appeared to be due to IL-1, pre-incubation of SBF with neutralizing antibodies against IL-1 alpha and IL-1 beta reducing the effect significantly. Substantial PBL attractant activity was present also in the SBF from 1-3 h and 24 h post-irradiation samples in both patient groups and again, IL-1 neutralizing antibodies reduced this in the 1-3 h and 24 h CAD samples. In addition, neutralizing antibodies against IL-6 and IL-8 reduced the activity in the 24 h PLE samples significantly and although not fully conclusive in the case of IL-1, these data suggest that IL-6, IL-8 and possibly IL-1 may be involved in the induction of PBL infiltrates, and perhaps other events, in both PLE and CAD.
authors.affiliation: St John's Institute of Dermatology, St Thomas's Hospital London, and The National Institute for Biological Standards and Control, Potters Bar, UK.
authors.forename: P
authors.initials: P
authors.lastname: Norris
authors.forename: K
authors.initials: K
authors.lastname: Bacon
authors.forename: C
authors.initials: C
authors.lastname: Bird
authors.forename: J
authors.initials: J
authors.lastname: Hawk
authors.forename: R
authors.initials: R
authors.lastname: Camp
mesh_terms: D000328:Adult; D000368:Aged; D019138:Antibodies, Blocking / Q000276:immunology; D001768:Blister / Q000276:immunology; D002455:Cell Division; D002465:Cell Movement / Q000276:immunology; D002630:Chemotactic Factors / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology* / Q000528:radiation effects; D005122:Exudates and Transudates / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology*; D015850:Interleukin-6 / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000276:immunology*; D012867:Skin / Q000276:immunology / Q000528:radiation effects
chemical_list: D019138:Antibodies, Blocking; D002630:Chemotactic Factors; D007074:Immunoglobulin G; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8