Effects of chronic liver diseases on mitochondrial DNA transcription and replication in human liver.
To evaluate the effects of chronic liver diseases on mitochondrial DNA (mtDNA) transcription and replication, nuclear respiratory factor-1 (NRF-1) mRNA, mitochondrial transcription factor A (mtTFA) mRNA, a RNA component of ribonuclease (RNase) for mitochondrial RNA processing (MRP), mitochondrial cytochrome b mRNA, and mtDNA were measured in normal, chronically viral-hepatitic and cirrhotic human livers. The mRNA levels of the regulatory factors for mitochondrial gene (NRF-1 and mtTFA) and cytochrome b were significantly increased by chronic hepatitis (160, 280, and 175%, respectively) compared with those in normal livers, but were not different between cirrhotic and normal livers. On the other hand, concentrations of mtDNA and RNA component of RNase MRP were not different among normal, chronically hepatitic, and cirrhotic livers. These results suggest that either persistent hepatitis viral infection or repeated cell necrosis and regeneration in chronically hepatitic liver may be associated with increase in mtDNA transcription.
Kotake
K
K
Department of Surgery II, Nagoya University, School of Medicine, Japan.
Nonami
T
T
Kurokawa
T
T
Nakao
A
A
Murakami
T
T
Shimomura
Y
Y
D000328:Adult; D000368:Aged; D002908:Chronic Disease; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D008107:Liver Diseases / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000235:genetics* / Q000473:pathology; D051265:NF-E2-Related Factor 1; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014158:Transcription, Genetic; D029867:Xenopus Proteins
D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D051265:NF-E2-Related Factor 1; C495633:NRF1 protein, human; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D012333:RNA, Messenger; D015534:Trans-Activators; C097078:XL-MTTFA protein, Xenopus; D029867:Xenopus Proteins; D012260:Ribonucleases