title: Fast laser-induced solute release from liposomes sensitized with photochromic lipid: effects of temperature, lipid host, and sensitizer concentration.
abstract: Liposomes of gel-phase phospholipid have been prepared containing a photochromic lipid sensitizer. A fast UV laser pulse isomerizes the sensitizer destabilizing the lipid bilayer structure and causing release of trapped solute. The kinetics of solute release have been investigated as a function of host lipid chain length, sensitizer concentration, and temperature, and the limits of liposome stability have been established. At low concentrations of sensitizer, pulsed laser irradiation induces some solute release when continuous UV illumination is ineffective. Although rates of solute release usually increase with temperature, at low sensitizer concentration in a rigid host, leakage at first increases but then decreases rapidly above a threshold temperature. The results presented are relevant to the design of photostimulated drug delivery systems and to potential applications of photosensitive liposomes as caging agents for biological effectors.
authors.affiliation: Science Research Institute, University of Salford, Salford, M5 4WT, United Kingdom. r.h.bisby@biosci.salford.ac.uk
authors.forename: R H
authors.initials: RH
authors.lastname: Bisby
authors.forename: C
authors.initials: C
authors.lastname: Mead
authors.forename: A C
authors.initials: AC
authors.lastname: Mitchell
authors.forename: C G
authors.initials: CG
authors.lastname: Morgan
mesh_terms: D004307:Dose-Response Relationship, Radiation; D016503:Drug Delivery Systems; D005452:Fluoresceins; D007834:Lasers; D008051:Lipid Bilayers / Q000528:radiation effects*; D008081:Liposomes / Q000528:radiation effects*; D010507:Periodicity; D010539:Permeability; D010713:Phosphatidylcholines / Q000528:radiation effects*; D010782:Photolysis; D017319:Photosensitizing Agents / Q000528:radiation effects*; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D005452:Fluoresceins; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; C053913:1,2-bis(4-(n-butyl)phenylazo-4'-phenylbutyroyl)phosphatidylcholine; C007740:fluorexon