Serotonin depolarizes hippocampal interneurones in the rat stratum oriens by interaction with 5HT2 receptors.
Patch-clamp recording techniques were used to examine the effect of serotonin (5HT) upon interneurones contained in the stratum oriens layer of hippocampal slices. Bath application of 1-20 microM 5HT depolarized neurones by the induction of an inward current at -60 mV. This inward current was Na+-dependent in nature, was mimicked by the 5HT2 receptor agonist 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and was inhibited by pre-incubation with the 5HT2 receptor antagonist ritanserin.
Lee
K
K
Parke-Davis Neuroscience Research Centre, Cambridge University Forvie Site, UK. kevin.lee2@wl.com
Dixon
A K
AK
Pinnock
R D
RD
D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D066298:In Vitro Techniques; D007215:Indophenol / Q000031:analogs & derivatives / Q000494:pharmacology; D007395:Interneurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000502:physiology; D011985:Receptors, Serotonin / Q000502:physiology*; D016713:Ritanserin / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology* / Q000502:physiology; D012702:Serotonin Antagonists / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
D011810:Quinoxalines; D011963:Receptors, GABA-A; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; C113896:dimethoxy-4-indophenyl-2-aminopropane; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D016713:Ritanserin; D012701:Serotonin; D013779:Tetrodotoxin; D007215:Indophenol