In vivo testing of Enterogenin for a mutagenic activity of bone marrow hemopoietic tissue of mice.
The purpose of the present study was to test Enterogenin for genotoxicity. The micronuclearic test of Schmid W. was used. Enterogenin was introduced intraperitoneally, once and/or twice in two doses on male and female mice of the BDF 1 hybrid line. The positive controls were injected with clastogenic agent. Bone marrow smear preparations were made, determining the ratio between the normochromic erythrocytes (NCE) and polychromic erythrocytes (PCE), and the number of micronuclearic erythrocytes (MNE). Enterogenin has no genotoxic effect on the hemopoietic tissue of bone marrow of mice the ratio NCE/PCE being within the reference values. The preparation showed no clastogenic effect (p > 0.05), compared to the controls, as the number of PCE with micronuclei did not change 24, 48 and 72 hours after treatment with the two studied doses.
Drakopoylos
D
D
Department of Physiology, Higher Medical Institute, Plovdiv, Bulgaria.
Zaprianov
Z
Z
Andreev
S
S
Trifonov
B
B
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D002460:Cell Line; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009154:Mutation / Q000187:drug effects*; D009842:Oligopeptides / Q000633:toxicity*
D009842:Oligopeptides; C099776:enterogenin; D000249:Adenosine Monophosphate