Cloning of a second proopiomelanocortin cDNA from the pituitary of the sturgeon, Acipenser transmontanus.
A recent study on the pituitary of the sturgeon, Acipenser transmontanus, resulted in the cloning of a cDNA that codes for the prohormone, proopiomelanocortin (POMC). This cDNA is designated sturgeon POMC A. Subsequent analysis of the sturgeon pituitary uncovered a second distinct POMC cDNA (sturgeon POMC B). In both sturgeon POMC cDNAs the open reading frame is 795 nucleotides in length. However, the two sturgeon POMC cDNAs differ at 26 amino acid positions in the opening frame. In addition, the 2 forms of POMC differ at 45 nucleotide positions within the open reading frame. The number and types of point mutations are compared in the 2 sturgeons POMC cDNAs, and the origin of the two POMC genes is discussed.
Alrubaian
J
J
University of Denver, Department of Biological Sciences, Colorado 80208, USA.
Danielson
P
P
Fitzpatrick
M
M
Schreck
C
C
Dores
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D020440:Gene Duplication; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010902:Pituitary Gland / Q000737:chemistry*; D017354:Point Mutation; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D011333:Pro-Opiomelanocortin