[Detection of antineutrophil cytoplasmic antibodies for the values of Wegener's granulomatosis diagnosis and activity judgment].
OBJECTIVE
To investigate the clinical significance of antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis (WG) and other diseases.


METHODS
Indirect immunofluorescence was applied to detect serum ANCA of 204 patients and 48 normal controls. Enzyme-linked immunosorbent assay (ELISA) was used to detect antimyeloperoxidase (MPO) antibodies.


RESULTS
The frequency of ANCA in sera of WG patients was 75% and significantly higher than those in sera of other groups (P < 0.05). ELISA demonstrated that MPO was the major antigen recognized by perinuclear ANCA (P-ANCA). Cytoplasmic ANCA (C-ANCA) was always positive and high titers in sera of active WG patients, but turned to negative or low titers in remission.


CONCLUSIONS
These results suggested that C-ANCA was specific for WG and was a marker for disease activity in WG. P-ANCA wasn't specific for WG, but related to vasculitis.
Zhang
X
X
PUMC Hospital, Beijing.
Jiang
M
M
Chui
J
J
Wang
H
H
Ma
J
J
D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D015415:Biomarkers; D019084:Fluorescent Antibody Technique, Indirect; D014890:Granulomatosis with Polyangiitis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans
D019268:Antibodies, Antineutrophil Cytoplasmic; D015415:Biomarkers