title: Intracellular Ca2+ responses to nucleotides, peptides, amines, amino acids and prostaglandins in cultured pituicytes from adult rat neurohypophysis.
abstract: The present study aimed to investigate the reactivity of cultured pituicytes from adult neurohypophysis to various bioactive substances using Ca2+ indicator dye Fura-2. A transient increase of intracellular Ca2+ [Ca2+]i was observed when pituicytes were treated with nucleotides (ATP, ADP, UTP, and UDP) and amines (5-HT2 and alpha2-agonist). Treatment with peptides such as endothelin-1 (ET-1), endothelin-3 (ET-3), bradykinin (BK), vasopressin (AVP), and angiotensin II (Ang II) also induced [Ca2+]i increase in pituicytes. Prostaglandin E2 (PGE2) and F2alpha (PGF2alpha) increased [Ca2+]i, but amino acids of GABA, glutamate (Glu), and taurine had no effect. Serum-free culture condition augmented [Ca2+]i responses to ATP, Ang II and 5-HT within 24 h. These results indicate that pituicytes express many of receptors for neurotransmitters or neuromodulators.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakai
authors.forename: K
authors.initials: K
authors.lastname: Furuya
authors.forename: S
authors.initials: S
authors.lastname: Miyata
authors.forename: T
authors.initials: T
authors.lastname: Kiyohara
mesh_terms: D000588:Amines / Q000494:pharmacology*; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D007091:Image Processing, Computer-Assisted; D009711:Nucleotides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011453:Prostaglandins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000588:Amines; D000596:Amino Acids; D009711:Nucleotides; D010455:Peptides; D011453:Prostaglandins; D002118:Calcium