[DNA polymorphism applied to paternity testing. Analysis of 877 cases].
Paternity testing is based on biological analyses that have drastically developed during the past 20 years. According to scientific developments, paternity testing was based on red blood groups studies, the analysis of red cell enzymes and plasma proteins polymorphisms, the typing of the HLA-A and B antigens, and the DNA polymorphism in its various forms. This study aims at comparing the various analyses so that each becomes a reference for the others, in order to determine (i) the acceptable level of the paternity probability evaluation, and (ii) the exclusion rules according to new technologies. The relative performances of the various combinations of tests is analyzed in this report, based on a study of 877 cases. Thanks to studies based on the gene amplification of micro-satellites, the efficiency of this technique has been proved: it is however necessary to identify the limits of the tests and to know how to deal with those cases which require further analysis. Beyond the most efficient biological analysis, it is very important to think about paternity testing as a process in which biological tests are only one step.
Van Huffel
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V
Institut national de la transfusion sanguine, Paris, France.
Rouger
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P
D000328:Adult; D001789:Blood Group Antigens / Q000235:genetics; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005602:France; D005819:Genetic Markers; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D018598:Minisatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D012189:Retrospective Studies
D001789:Blood Group Antigens; D015342:DNA Probes; D005819:Genetic Markers; D006680:HLA Antigens; D004247:DNA