Distribution of calcium sensing receptor in rats: an immunohistochemical study.
OBJECTIVE
To investigate the organ distribution of calcium sensing receptor (CaR) in rats by immunohistochemical method.


METHODS
CaR was identified immunohistochemically in the rat tissues using specific anti-CaR antiserum raised in New Zealand white rabbits immunized with a conjugate of synthetic CaR peptide (186-204) with bovine serum albumin. Immunohistochemical analysis was performed by avidin-biotin complex method.


RESULTS
CaR immunoreactivity was visualized in the central nervous system, anterior pituitary, gastric mucosa, small intestine and colon, Auerbach,s and Meissner,s gastric nervous branch, small intestine and colon, pancreas, adrenal medulla, kidney and testis. When using antiserum preincubated with synthetic CaR peptide (186-206) or kidney homogenates, no significant stain of kidney was detected.


CONCLUSIONS
The findings suggest that CaR is widely distributed and that the method used is valuable in studying the distribution of caR in rat.
Mitsuma
T
T
Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi 4801195 Japan.
Rhue
N
N
Kayama
M
M
Mori
Y
Y
Adachi
K
K
Yokoi
Y
Y
Ping
J
J
Nogimori
T
T
Hirooka
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium; D002490:Central Nervous System / Q000737:chemistry; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing; D011956:Receptors, Cell Surface / Q000032:analysis*
D044169:Receptors, Calcium-Sensing; D011956:Receptors, Cell Surface; C473605:extracellular calcium cation-sensing receptor, rat; D002118:Calcium