[The effects of E2 and it's metabolites on the proliferation of rat anterior pituitary cells in vitro].
OBJECTIVE
The present work is determined to observe the effects of E2 (estradiol), 2-OHE1 (2-hydroxyestrone), 2-OHE2 (2-hydroxyestrodiol) on the proliferation of rat anterior pituitary cells (APC) in vitro by laser scanning confocal microscopy.


RESULTS
10(-6) mol/L E2 stimulated the growth of APC. After 2 days of incubation with E2, the DNA content of APC increased to 1.3 times of the control group (P < 0.01). 10(-6) mol/L 2-OHE2 (other than 2-OHE1) stimulated proliferative activity of APC and inhibited the inhibitory effect of peribidil (10(-5) mol/L), a dopamine receptor agonist, on the poliferative activity of rat APC.
Di
A
A
Institute of Basic Medical Sciences, CAMS, Beijing.
Xu
R
R
Wu
X
X
Huang
M
M
Shan
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018492:Dopamine Antagonists / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006894:Hydroxyestrones / Q000494:pharmacology*; D008297:Male; D010891:Piribedil / Q000037:antagonists & inhibitors; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018492:Dopamine Antagonists; D006894:Hydroxyestrones; D004958:Estradiol; C001390:2-hydroxyestradiol; D010891:Piribedil; C024980:2-hydroxyestrone