Structure of the human VIPR2 gene for vasoactive intestinal peptide receptor type 2.
The VPAC(2) (vasoactive intestinal peptide (VIP)(2)) receptor is a seven-transmembrane spanning G protein-coupled receptor which responds similarly to VIP and pituitary adenylate cyclase activating polypeptide (PACAP) in stimulating cAMP production. Recently, we reported the localisation of the human VPAC(2) receptor gene (VIPR2) to chromosome 7q36.3 (Mackay, M. et al. (1996) Genomics 37, 345-353). Here, we describe the characterisation of the VIPR2 gene structure and promoter region. The VIPR2 gene is encoded by 13 exons, the initiator codon of the 438 amino acid open reading frame is located in exon 1 and the termination signal and a poly-adenylation signal sequence are located in exon 13. The 5' untranslated region extends 187 bp upstream of the initiator codon and is extremely GC-rich (80%). The poly-adenylation signal is located 2416 bp downstream of the stop codon. Intron sizes range from 68 bp (intron 11) to 45 kb (intron 4) and the human gene spans 117 kb.
Lutz
E M
EM
MRC Brain Metabolism Unit, University Department of Neuroscience, Edinburgh, UK. elutz@srv1.bmu.mrc.ac.uk
Shen
S
S
Mackay
M
M
West
K
K
Harmar
A J
AJ
D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003596:Cytosine; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005091:Exons; D019204:GTP-Binding Proteins / Q000378:metabolism; D006147:Guanine; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D018005:Receptors, Vasoactive Intestinal Peptide / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D020121:5' Untranslated Regions; D018076:DNA, Complementary; D018005:Receptors, Vasoactive Intestinal Peptide; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D014157:Transcription Factors; C495509:VIPR2 protein, human; D006147:Guanine; D003596:Cytosine; D019204:GTP-Binding Proteins