New techniques for the rapid characterization of oligonucleotides by mass spectrometry.
Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors < 0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.
McCloskey
J A
JA
Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112-5820, USA.
Whitehill
A B
AB
Rozenski
J
J
Qiu
F
F
Crain
P F
PF
D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis*
D009841:Oligonucleotides