Comparison of the roles of the C1a and C1b domains of protein kinase C alpha in ligand induced translocation in NIH 3T3 cells.
To explore the relative roles of the two C1 domains of protein kinase C alpha (PKC alpha) in the response to phorbol esters and related analogs, we mutated the individual C1 domains, expressed the mutated PKC alpha in NIH 3T3 cells, and then examined the ability of ligands to induce its translocation to the membrane. The C1a and C1b domains play equivalent roles for translocation in response to phorbol 12-myristate 13-acetate, mezerein, and (-)octylindolactam V. These results contrast with those previously reported for PKC delta, suggesting that the domains play different roles in different PKC isoforms.
Bögi
K
K
Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, MD 20892-4255, USA.
Lorenzo
P S
PS
Acs
P
P
Szállási
Z
Z
Wagner
G S
GS
Blumberg
P M
PM
D016475:3T3 Cells / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D004224:Diterpenes; D007211:Indoles / Q000494:pharmacology; D007527:Isoenzymes / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D007769:Lactams / Q000494:pharmacology; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010766:Phosphorylation; D011493:Protein Kinase C / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014178:Translocation, Genetic / Q000235:genetics*
D004224:Diterpenes; D007211:Indoles; D007527:Isoenzymes; D007769:Lactams; D013729:Terpenes; C086014:7-octylindolactam V; C011309:mezerein; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate