title: Natural antioxidant, chlorogenic acid, protects against DNA breakage caused by monochloramine.
abstract: Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with a half inhibition of 67.4 microM. Chlorogenic acid reacted with monochloramine in a time-dependent manner, and the reaction rate increased with decreasing pH. These results suggest that chlorogenic acid prevents genotoxicity of monochloramine in gastric mucosa.
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Japan. shibata@life.shimane-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: Y
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: M
authors.initials: M
authors.lastname: Oka
authors.forename: Y
authors.initials: Y
authors.lastname: Kono
mesh_terms: D000643:Ammonium Chloride / Q000737:chemistry; D000975:Antioxidants / Q000494:pharmacology*; D002700:Chloramines / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004269:DNA, Bacterial / Q000737:chemistry / Q000187:drug effects; D016480:Helicobacter pylori / Q000187:drug effects / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D002700:Chloramines; D004269:DNA, Bacterial; D000643:Ammonium Chloride; D002726:Chlorogenic Acid; C030816:chloramine