Allele resolution of HLA-A using oligonucleotide probes in a two-stage typing strategy.
High-resolution polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) typing methods for HLA-A identification have been established. The four systems, which operate independently of each other, are intended for use as secondary typing systems following HLA-A identification with a medium-resolution PCR-SSOP technique. The systems, all using digoxigenin-labelled probes, are based on group specific amplifications for resolution of: i) HLA-A*29 & -A*33; ii) HLA-A*24 & -A*30; and iii) HLA-A*26, -A*25, -A*11, -A*34, -A*66 and -A*68 alleles, respectively. The fourth system, for the detection of HLA-A*02 alleles, is a modification of a previously reported PCR-SSOP subtyping system. The methods have been applied to individuals from the local bone marrow registry and HLA-A allele frequencies for the Northern Ireland population have been established.
Williams
F
F
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Ireland.
Meenagh
A
A
Maxwell
A P
AP
Middleton
D
D
D000483:Alleles; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D007494:Ireland; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
D015234:HLA-A Antigens; D015345:Oligonucleotide Probes