Radiation-induced apoptosis and cell cycle progression in Jurkat T cells.
The purpose of this study was to explore the connection between radiation-induced apoptosis and progression of cells through the phases of the cell cycle. Cells of the human T-cell line Jurkat were separated by centrifugal elutriation into populations enriched in G(1)-, S- and G(2)/M-phase cells before irradiation. After a dose of 20 Gy, the onset of massive apoptosis occurred at about 6 h in all populations regardless of the phase of the cell cycle in which they were irradiated. In contrast, after 2 Gy, cells died at various times after a pronounced G(2)/M-phase arrest. These results indicate that radiation-induced apoptosis can occur independently of cell cycle arrest and that the time for onset of apoptosis may be dependent on the radiation dose.
Syljuâsen
R G
RG
Department of Radiation Oncology and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California 90095, USA.
McBride
W H
WH
D017209:Apoptosis / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects; D002498:Centrifugation; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D019169:Jurkat Cells / Q000473:pathology / Q000528:radiation effects*; D011836:Radiation Tolerance; D013997:Time Factors