Cytotoxic T lymphocytes to Ebola Zaire virus are induced in mice by immunization with liposomes containing lipid A.
An eight amino acid sequence (TELRTFSI) present in the carboxy terminal end (aa 577-584) of membrane-anchored GP, the major structural protein of Ebola virus, was identified as an H-2k-specific murine cytotoxic T cell epitope. Cytotoxic T lymphocytes (CTLs) to this epitope were induced by immunizing B10.BR mice intravenously with either irradiated Ebola virus or with irradiated Ebola virus encapsulated in liposomes containing lipid A. The CTL response induced by irradiated Ebola virus could not be sustained after the second round of in vitro stimulation of immune splenocytes with the peptide, unless the irradiated virus was encapsulated in liposomes containing lipid A. The identification of an Ebola GP-specific CTL epitope and the requirement of liposomal lipid A for CTL memory recall responses could prove to be a promising approach for developing a vaccine against Ebola virus infection.
Rao
M
M
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA. dr._mangala_rao@wrsmtp-ccmail.army.mil
Matyas
G R
GR
Grieder
F
F
Anderson
K
K
Jahrling
P B
PB
Alving
C R
CR
D000818:Animals; D002522:Chlorocebus aethiops; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D029043:Ebolavirus / Q000276:immunology* / Q000528:radiation effects; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D007117:Immunization, Secondary; D008050:Lipid A / Q000008:administration & dosage / Q000276:immunology*; D008081:Liposomes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D008050:Lipid A; D008081:Liposomes; D010446:Peptide Fragments; D015678:Viral Structural Proteins; D014765:Viral Vaccines