Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent.
Procyanidins present in grape seeds are known to exert anti-inflammatory, anti-arthritic and anti-allergic activities, prevent skin aging, scavenge oxygen free radicals and inhibit UV radiation-induced peroxidation activity. Since most of these events are associated with the tumor promotion stage of carcinogenesis, these studies suggest that grape seed polyphenols and the procyanidins present therein could be anticarcinogenic and/or anti-tumor-promoting agents. Therefore, we assessed the anti-tumor-promoting effect of a polyphenolic fraction isolated from grape seeds (GSP) employing the 7,12-dimethylbenz[a]anthracene (DMBA)-initiated and 12-O-tetradecanoylphorbol 13-acetate (TPA)-promoted SENCAR mouse skin two-stage carcinogenesis protocol as a model system. Following tumor initiation with DMBA, topical application of GSP at doses of 0.5 and 1.5 mg/mouse/application to the dorsal initiated mouse skin resulted in a highly significant inhibition of TPA tumor promotion. The observed anti-tumor-promoting effects of GSP were dose dependent and were evident in terms of a reduction in tumor incidence (35 and 60% inhibition), tumor multiplicity (61 and 83% inhibition) and tumor volume (67 and 87% inhibition) at both 0.5 and 1.5 mg GSP, respectively. Based on these results, we directed our efforts to separate and identify the individual polyphenols present in GSP and assess their antioxidant activity in terms of inhibition of epidermal lipid peroxidation. Employing HPLC followed by comparison with authentic standards for retention times in HPLC profiles, physiochemical properties and spectral analysis, nine individual polyphenols were identified as catechin, epicatechin, procyanidins B1-B5 and C1 and procyanidin B5-3'-gallate. Five of these individual polyphenols with evident structural differences, namely catechin, procyanidin B2, procyanidin B5, procyanidin C1 and procyanidin B5-3'-gallate, were assessed for antioxidant activity. All of them significantly inhibited epidermal lipid peroxidation, albeit to different levels. A structure-activity relationship study showed that with an increase in the degree of polymerization in polyphenol structure, the inhibitory potential towards lipid peroxidation increased. In addition, the position of linkage between inter-flavan units also influences lipid peroxidation activity; procyanidin isomers with a 4-6 linkage showed stronger inhibitory activity than isomers with a 4-8 linkage. A sharp increase in the inhibition of epidermal lipid peroxidation was also evident when a gallate group was linked at the 3'-hydroxy position of a procyanidin dimer. Procyanidin B5-3'-gallate showed the most potent antioxidant activity with an IC(50) of 20 microM in an epidermal lipid peroxidation assay. Taken together, for the first time these results show that grape seed polyphenols possess high anti-tumor-promoting activity due to the strong antioxidant effect of procyanidins present therein. In summary, grape seed polyphenols in general, and procyanidin B5-3'-gallate in particular, should be studied in more detail to be developed as cancer chemopreventive and/or anticarcinogenic agents.
Zhao
J
J
Center for Cancer Causation and Prevention, AMC Cancer Research Center, Denver, CO 80214, USA.
Wang
J
J
Chen
Y
Y
Agarwal
R
R
D015127:9,10-Dimethyl-1,2-benzanthracene; D000096:Acetone; D000279:Administration, Cutaneous; D000818:Animals; D016588:Anticarcinogenic Agents / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000494:pharmacology; D044946:Biflavonoids; D002392:Catechin / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D003043:Cocarcinogenesis; D004817:Epidermis / Q000187:drug effects; D005260:Female; D005419:Flavonoids; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D018632:Mice, Inbred SENCAR; D008861:Microsomes / Q000378:metabolism; D015394:Molecular Structure; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000737:chemistry*; D011108:Polymers / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D044945:Proanthocyanidins; D019685:Rosales; D012639:Seeds / Q000737:chemistry; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate; D014810:Vitamin E / Q000494:pharmacology
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D044946:Biflavonoids; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; D044945:Proanthocyanidins; C479586:procyanidin B5 3'-O-gallate; C121404:procyanidin B5-3'-gallate; D000096:Acetone; D014810:Vitamin E; C479580:procyanidin B2; D015127:9,10-Dimethyl-1,2-benzanthracene; D002392:Catechin; D013755:Tetradecanoylphorbol Acetate; D001205:Ascorbic Acid; C479582:procyanidin B5