title: A Drosophila Axin homolog, Daxin, inhibits Wnt signaling.
abstract: The vertebrate Axin protein, the product of the mouse fused gene, binds to beta-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in Drosophila, Daxin. Using double-stranded RNA interference, we generated loss-of-function phenotypes that are similar to overexpression of the Drosophila Wnt gene wingless (wg). Overexpression of Daxin produces phenotypes similar to loss of wg. In addition, we show that Daxin overexpression can modify phenotypes elicited by wg and another Drosophila Wnt gene, DWnt-2. Using immunoprecipitation of endogenous Daxin protein from embryos we show that Daxin interacts with Armadillo and Zeste-white 3. The loss-of-function and overexpression phenotypes show that Daxin, like its mammalian counterpart, acts as a negative regulator of wg/Wnt signaling.
authors.affiliation: Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: K
authors.initials: K
authors.lastname: Willert
authors.forename: C Y
authors.initials: CY
authors.lastname: Logan
authors.forename: A
authors.initials: A
authors.lastname: Arora
authors.forename: M
authors.initials: M
authors.lastname: Fish
authors.forename: R
authors.initials: R
authors.lastname: Nusse
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D051186:Armadillo Domain Proteins; D060466:Axin Protein; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian; D020224:Expressed Sequence Tags; D005123:Eye / Q000254:growth & development; D005124:Eye Abnormalities / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005821:Genetic Techniques; D038362:Glycogen Synthase Kinase 3; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015534:Trans-Activators; D014157:Transcription Factors; D014921:Wings, Animal / Q000254:growth & development; D051155:Wnt1 Protein; D051154:Wnt2 Protein
chemical_list: C495305:ARM protein, Drosophila; D048868:Adaptor Proteins, Signal Transducing; D051186:Armadillo Domain Proteins; D060466:Axin Protein; C118000:Axn protein, Drosophila; D002352:Carrier Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051155:Wnt1 Protein; D051154:Wnt2 Protein; C495251:Wnt2 protein, Drosophila; C074622:wg protein, Drosophila; D012313:RNA; D017346:Protein Serine-Threonine Kinases; C080335:Sgg protein, Drosophila; D038362:Glycogen Synthase Kinase 3