ADAM-TS5, ADAM-TS6, and ADAM-TS7, novel members of a new family of zinc metalloproteases. General features and genomic distribution of the ADAM-TS family.
We report the primary structure of three novel, putative zinc metalloproteases designated ADAM-TS5, ADAM-TS6, and ADAM-TS7. All have a similar domain organization, comprising a preproregion, a reprolysin-type catalytic domain, a disintegrin-like domain, a thrombospondin type-1 (TS) module, a cysteine-rich domain, a spacer domain without cysteine residues, and a COOH-terminal TS module. These genes are differentially regulated during mouse embryogenesis and in adult tissues, with Adamts5 highly expressed in the peri-implantation period in embryo and trophoblast. These proteins are similar to four other cognate gene products, defining a distinct family of human reprolysin-like metalloproteases, the ADAM-TS family. The other members of the family are ADAM-TS1, an inflammation-induced gene, the procollagen I/II amino-propeptide processing enzyme (PCINP, ADAM-TS2), and proteins predicted by the KIAA0366 and KIAA0688 genes (ADAM-TS3 and ADAM-TS4). Individual ADAM-TS members differ in the number of COOH-terminal TS modules, and some have unique COOH-terminal domains. The ADAM-TS genes are dispersed in human and mouse genomes.
Hurskainen
T L
TL
Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Hirohata
S
S
Seldin
M F
MF
Apte
S S
SS
D051722:ADAM Proteins; D000071096:ADAMTS Proteins; D000071116:ADAMTS5 Protein; D000071118:ADAMTS7 Protein; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019483:Disintegrins; D016678:Genome; D015894:Genome, Human; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016415:Sequence Alignment
D019483:Disintegrins; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human; D000071118:ADAMTS7 Protein; C121534:ADAMTS7 protein, human; C498489:Adamts5 protein, mouse; C121533:Adamts6 protein, human; D008666:Metalloendopeptidases