Anti-metastatic activity of curcumin and catechin.
The inhibitory effects of curcumin and catechin on lung metastasis induced by B16F-10 melanoma cells were studied in female C57BL/6 mice. Curcumin and catechin significantly (P < 0.001) inhibited lung tumour formation (89.3% and 82.2%, respectively) and significantly increased the life span (143.9% and 80.8%, respectively). Moreover, lung collagen hydroxyproline and serum sialic acid levels were found to be significantly (P < 0.001) lower in treated animals compared to the untreated controls. Curcumin and catechin treatment (10 microg/ml) significantly inhibited the invasion of B16F-10 melanoma cells across the collagen matrix of the Boyden chamber. Gelatin zymographic analysis of the trypsin-activated B16F-10 melanoma cells sonicate revealed that curcumin- and catechin-treated zymograms did not show any metalloproteinase activity. Curcumin and catechin treatment did not inhibit the motility of B16F-10 melanoma cells across a polycarbonate filter in vitro. These findings suggest that curcumin and catechin inhibit the invasion of B16F-10 melanoma cells by inhibition of metalloproteinases, thereby inhibiting lung metastasis.
Menon
L G
LG
Amala Cancer Research Centre, Trichur, India.
Kuttan
R
R
Kuttan
G
G
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology*; D005260:Female; D005780:Gelatin / Q000378:metabolism; D006909:Hydroxyproline / Q000032:analysis; D008175:Lung Neoplasms / Q000401:mortality / Q000517:prevention & control* / Q000556:secondary; D008546:Melanoma, Experimental / Q000401:mortality / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D019158:N-Acetylneuraminic Acid / Q000097:blood; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D015996:Survival Rate; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002392:Catechin; D005780:Gelatin; D003094:Collagen; D019158:N-Acetylneuraminic Acid; D003474:Curcumin; D006909:Hydroxyproline