title: Effect of inflammatory mediators on the electrophysiology of the human oviduct.
abstract: The effects of histamine and other inflammatory mediators on the electrophysiology and intracellular free calcium ([Ca(2+)](i)) of human oviductal epithelial cells, grown as a polarized layer in primary culture, were studied. Transepithelial potential difference (PD) and short-circuit current (I(scc)) were recorded using a modified Ussing chamber. Resistance (R) was calculated from the measurements of PD and I(scc). Basally applied histamine produced transient increases in PD and I(scc) with a small decrease in R. The histamine effect was reduced by triprolidine (H(1) receptor antagonist) but was unaffected by H(2) (ranitidine) or H(3) (thioperamide) receptor antagonists. Blockers of Na(+), K(+), or Na(+)/K(+)/2Cl(-) channels did not affect histamine action. Blockers of Cl(-)/HCO(3)(-) channels or Ca(2+) channels reduced the histamine effect. Platelet activating factor (PAF), applied apically, increased PD and I(scc). Histamine produced a transient increase in fluorescence of Fura 2-AM dye, indicating an increase in [Ca(2+)](i). Triprolidine pretreatment inhibited histamine-stimulated [Ca(2+)](i) increase. Cimetidine, (H(2) receptor antagonist), ranitidine, or thioperamide reduced the histamine effect. Histamine increased contractions of both circular and longitudinal smooth muscles in oviduct segments, an effect that was antagonized by triprolidine or thioperamide but not by ranitidine. Histamine's action on Ca(2+) and Cl(-) movements may adversely affect oviductal fluid production and decrease fertility. PAF's effects on Cl(-) movements may be important for normal embryo transport.
authors.affiliation: Department of Biology, University of York, York YO21 5YW, United Kingdom. sjd13@york.ac.uk
authors.forename: S J
authors.initials: SJ
authors.lastname: Downing
authors.forename: E L
authors.initials: EL
authors.lastname: Chambers
authors.forename: S D
authors.initials: SD
authors.lastname: Maguiness
authors.forename: A
authors.initials: A
authors.lastname: Watson
authors.forename: H J
authors.initials: HJ
authors.lastname: Leese
mesh_terms: D000527:Alprostadil / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D015232:Dinoprostone / Q000494:pharmacology; D004553:Electric Conductivity; D004594:Electrophysiology; D004847:Epithelial Cells / Q000502:physiology; D005187:Fallopian Tubes / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D007473:Ion Channels / Q000037:antagonists & inhibitors; D007975:Leukotriene B4 / Q000494:pharmacology; D008564:Membrane Potentials; D010880:Piperidines / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology
chemical_list: D002712:Chlorides; D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D007473:Ion Channels; D010880:Piperidines; D010972:Platelet Activating Factor; D007975:Leukotriene B4; D006632:Histamine; D000527:Alprostadil; C052075:thioperamide; D015232:Dinoprostone; D002118:Calcium