Sampling of low molecular weight iron by microdialysis following spinal cord injury.
We combined the use of desferrioxamine as an iron chelator, microdialysis sampling, and iron analysis by atomic absorption spectroscopy to measure extracellular levels of low molecular weight (LMW) iron in vivo in the spinal cord. Low molecular weight iron is free iron plus iron bound to small molecules. We show that the extracellular LMW-iron concentration is not increased significantly in situ by trauma to the rat spinal cord, suggesting that an extracellular elevation in LMW-iron at the site of injury is not the major initiating factor for lipid peroxidation following spinal cord injury.
de Castro
R
R
Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston 77555, USA.
Alcock
N W
NW
McAdoo
D J
DJ
D000818:Animals; D003676:Deferoxamine; D007501:Iron / Q000134:cerebrospinal fluid*; D015227:Lipid Peroxidation; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid*; D013997:Time Factors
D007501:Iron; D003676:Deferoxamine