Carbohydrate-dependent hemolytic activity of the conjugate composed of a C-type lectin, CEL-I, and an amphiphilic alpha-helical peptide, 4(3)-beta Ala2.
A lectin-cationic peptide conjugate, 4(3)-CEL-I, was prepared from an invertebrate C-type lectin, CEL-I, and an amphiphilic alpha-helical peptide, 4(3)-beta Ala2 [Ac-(Leu-Ala-Arg-Leu)3-beta Ala2]. When 4(3)-CEL-I was incubated with rabbit erythrocytes, hemolysis was observed, especially at basic pH. Inhibition experiment using some carbohydrates suggested that hemolytic activity of 4(3)-CEL-I was caused by the interaction between 4(3)-beta Ala2 portion in the conjugate and the lipid bilayer after binding to the carbohydrate chains on the cell surface by the lectin activity of CEL-I.
Hatakeyama
T
T
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University, Japan. thata@net.nagasaki-u.ac.jp
Kamine
T
T
Konishi
Y
Y
Kuwahara
H
H
Niidome
T
T
Aoyagi
H
H
D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D037102:Lectins / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D012616:Sea Cucumbers / Q000737:chemistry* / Q000187:drug effects
D002241:Carbohydrates; D037102:Lectins; D010455:Peptides; D011506:Proteins; C122030:acetyl(leucyl-alanyl-arginyl-leucyl)3-beta-alanyl-beta-alanine