Structure of rat gamma-tubulin and its binding to HP33.
Gamma-tubulin is localized at the microtubule organizing center and is thought to participate in the organizing of the microtubule network. In this study, we isolated a cDNA of rat gamma-tubulin. The rat gamma-tubulin cDNA encoded 451 amino acids, the same number as that of its counterpart in other vertebrates, and its structure was found to be highly conserved in vertebrates. In a previous work, we identified HP33 (hepatocarcinogenesis- and hepatocellular proliferation-related 33-kDa protein) that was localized at the centrosome of hepatic cells and that exhibited MAP-like activity. In vitro GST pull-down assay using highly purified recombinant HP33 and bacterially expressed gamma-tubulin demonstrated that HP33 bound to gamma-tubulin directly. These results suggest that HP33 is localized at the centrosome via association with both the microtubule and its minus end-specific component, gamma-tubulin.
Nakadai
T
T
Department of Biology, Faculty of Science, Chiba University, Japan.
Okada
N
N
Makino
Y
Y
Tamura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D016415:Sequence Alignment; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; D014404:Tubulin