title: Repair of cellular radiation damage in space under microgravity conditions.
abstract: The influence of microgravity on the repair of x-ray-induced DNA double-strand breaks was studied in the temperature-conditional repair mutant rad54-3 of diploid yeast Saccharomyces cerevisiae. Cells were exposed on the ground and kept at a low temperature until microgravity conditions were achieved. In orbit, they were incubated at the permissive temperature to allow repair. Before re-entry they were again cooled down and kept at a low temperature until final analysis. The experiment, which was flown on the shuttle Atlantis on flight STS-76 (SMM-03), showed that repair of pre-formed DNA double-strand breaks in yeast is not impaired by microgravity.
authors.affiliation: Strahlenzentrum der Justus-Liebig-Universität, Giessen, Germany.
authors.forename: H D
authors.initials: HD
authors.lastname: Pross
authors.forename: J
authors.initials: J
authors.lastname: Kiefer
mesh_terms: D000337:Aerospace Medicine; D004260:DNA Repair; D004269:DNA, Bacterial / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008961:Models, Structural; D011829:Radiation Dosage; D012440:Saccharomyces / Q000235:genetics* / Q000528:radiation effects; D014893:Weightlessness; D014965:X-Rays
chemical_list: D004269:DNA, Bacterial