Meiotic chromosome dynamics dependent upon the rec8(+), rec10(+) and rec11(+) genes of the fission yeast Schizosaccharomyces pombe.
During meiosis homologous chromosomes replicate once, pair, experience recombination, and undergo two rounds of segregation to produce haploid meiotic products. The rec8(+), rec10(+), and rec11(+) genes of the fission yeast Schizosaccharomyces pombe exhibit similar specificities for meiotic recombination and rec8(+) is required for sister chromatid cohesion and homolog pairing. We applied cytological and genetic approaches to identify potential genetic interactions and to gauge the fidelity of meiotic chromosome segregation in the mutants. The rec8(+) gene was epistatic to rec10(+) and to rec11(+), but there was no clear epistatic relationship between rec10(+) and rec11(+). Reciprocal (crossover) recombination in the central regions of all three chromosomes was compromised in the rec mutants, but recombination near the telomeres was nearly normal. Each of the mutants also exhibited a high rate of aberrant segregation for all three chromosomes. The rec8 mutations affected mainly meiosis I segregation. Remarkably, the rec10 and rec11 mutations, which compromised recombination during meiosis I, affected mainly meiosis II segregation. We propose that these genes encode regulators or components of a "meiotic chromatid cohesion" pathway involved in establishing, maintaining, and appropriately releasing meiotic interactions between chromosomes. A model of synergistic interactions between sister chromatid cohesion and crossover position suggests how crossovers and cohesion help ensure the proper segregation of chromosomes in each of the meiotic divisions.
Krawchuk
M D
MD
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
DeVeaux
L C
LC
Wahls
W P
WP
D000782:Aneuploidy; D002503:Centromere / Q000235:genetics; D020090:Chromosome Segregation / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D020043:Genes, Essential; D005800:Genes, Fungal; D005838:Genotype; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D010750:Phosphoproteins; D011995:Recombination, Genetic / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D012689:Sequence Homology, Nucleic Acid; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development; D000097722:Cohesins
D005656:Fungal Proteins; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; C096136:REC10 protein, S pombe; C077081:REC8 protein, S pombe; D000097722:Cohesins