Synthesis and biological evaluation of modified DNA fragments for the study of nucleotide excision repair in E. coli.
Three new cholesterol-containing phosphoramidites where synthesized and used in automated synthesis of modified DNA fragments. These cholesterol lesions are good substrates for the E. coli UvrABC endonuclease. In vitro they are incised from damaged DNA with higher efficiency in respect with the cholesterol lesions previously published.
Monaco
V
V
Leiden Institute of Chemistry, The Netherlands.
van de Wetering
K I
KI
Meeuwenoord
N J
NJ
van den Elst
H A
HA
Stuivenberg
H R
HR
Visse
R
R
van der Kaaden
J C
JC
Moolenaar
G F
GF
Verhoeven
E E
EE
Goosen
N
N
van der Marel
G A
GA
van Boom
J H
JH
D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005069:Evaluation Studies as Topic; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology
D009841:Oligonucleotides