title: Synthesis and biological evaluation of modified DNA fragments for the study of nucleotide excision repair in E. coli.
abstract: Three new cholesterol-containing phosphoramidites where synthesized and used in automated synthesis of modified DNA fragments. These cholesterol lesions are good substrates for the E. coli UvrABC endonuclease. In vitro they are incised from damaged DNA with higher efficiency in respect with the cholesterol lesions previously published.
authors.affiliation: Leiden Institute of Chemistry, The Netherlands.
authors.forename: V
authors.initials: V
authors.lastname: Monaco
authors.forename: K I
authors.initials: KI
authors.lastname: van de Wetering
authors.forename: N J
authors.initials: NJ
authors.lastname: Meeuwenoord
authors.forename: H A
authors.initials: HA
authors.lastname: van den Elst
authors.forename: H R
authors.initials: HR
authors.lastname: Stuivenberg
authors.forename: R
authors.initials: R
authors.lastname: Visse
authors.forename: J C
authors.initials: JC
authors.lastname: van der Kaaden
authors.forename: G F
authors.initials: GF
authors.lastname: Moolenaar
authors.forename: E E
authors.initials: EE
authors.lastname: Verhoeven
authors.forename: N
authors.initials: N
authors.lastname: Goosen
authors.forename: G A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
mesh_terms: D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005069:Evaluation Studies as Topic; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology
chemical_list: D009841:Oligonucleotides