Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants.
The usefulness of thiophilic adsorption chromatography for the purification of rat IgG2b monoclonal antibodies has been evaluated. This approach has not shown specificity for immunoglobulins; therefore, to minimize potential interferences, the purification was carried out from supernatants of hybridomas grown in serum- and protein-free conditions. The protein purity of the six final antibody preparations assayed was always >/= 97%. In addition to the easiness of this procedure, which enables one-step antibody purification, the materials employed are rather inexpensive and milligram amounts of antibody can be recovered from 1 L of supernatant. Overall, the purification of rat IgG2b monoclonals under the conditions reported here offers an advantageous alternative to other more expensive and cumbersome methods.
Argüelles
M E
ME
Servicio de Citometría, Servicios Comunes de Investigación, Spain.
Alonso
M
M
García Suárez
M D
MD
Barneo
L
L
Sampedro
A
A
de los Toyos
J R
JR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002847:Chromatography, Agarose / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D006825:Hybridomas; D007074:Immunoglobulin G / Q000302:isolation & purification*; D011506:Proteins; D051381:Rats
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G; D011506:Proteins