Transient outward K+ currents across the plasma membrane of laticifer from Hevea brasiliensis.
Non-inactivating outward rectifying K+ channel currents have been identified in a variety of plant cell types and species. The present study of laticifer protoplasts from Hevea brasiliensis, cells which are specialized for stress response, has revealed, through a switch-clamp method, an outward rectifying current displaying rapid inactivation. The inactivation depended on the external K+ concentration and on the voltage. This current inactivation appeared clearly different from all those previously described in plant cells and it shared homology with current kinetics of animal Shaker family channels. These results, given the recent cloning of plant K+ channel beta-subunits, shed new light on possible plant K+ channel regulation.
Bouteau
F
F
Electrophysiologie des Membranes LPCMSP, Université Denis Diderot-Paris 7, France. bouteau@moka.ccr.jussieu.fr
Dellis
O
O
Rona
J P
JP
D002462:Cell Membrane / Q000502:physiology; D007840:Latex / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010940:Plant Proteins / Q000378:metabolism / Q000502:physiology*; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*; D024683:Potassium Channels, Tandem Pore Domain; D011189:Potassium Chloride / Q000494:pharmacology; D011523:Protoplasts / Q000378:metabolism / Q000502:physiology; D013997:Time Factors; D014197:Trees / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
D007840:Latex; D010940:Plant Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D011189:Potassium Chloride