Cell adhesion to tenascin-X mapping of cell adhesion sites and identification of integrin receptors.
Adhesive properties of tenascin-X (TN-X) were investigated using TN-X purified from bovine skin and recombinant proteins encompassing the RGD sequence located within the tenth fibronectin type-III domain, and the fibrinogen-like domain. Osteosarcoma (MG63) and bladder carcinoma cells (ECV304) cells were shown to adhere to purified TN-X, but did not spread and did not assemble actin stress fibers. Both cell types adhered to recombinant proteins harboring the contiguous fibronectin type-III domains 9 and 10 (FNX 9-10) but not to the FNX 10 domain alone. This adhesion to FNX 9-10 was shown to be mediated by alphavbeta3 integrin, was inhibited by RGD peptides and was strongly reduced in proteins mutated within the RGD site. As antibodies against alphavbeta3 integrin had no effects on cell adhesion to purified TN-X, we suggest that the RGD sequence is masked in intact TN-X. Cell attachment to the recombinant TN-X fibrinogen domain (FbgX) and to purified TN-X was greater for MG63 than for ECV304 cells. A beta1-containing integrin was shown to be involved in MG63 cell attachment to FbgX and to purified TN-X. Although the existence of other cell interaction sites is likely in this huge molecule, these similar patterns of adhesion and inhibition suggest that the fibrinogen domain might be a dominant site in the whole molecule.
Elefteriou
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Institut de Biologie et Chimie des Protéines, CNRS, Unité Propre de Recherche 412, Université Claude Bernard, Lyon, France.
Exposito
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JY
Garrone
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R
Lethias
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C
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009842:Oligopeptides; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012867:Skin / Q000166:cytology; D019063:Tenascin / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D016023:Integrins; D009842:Oligopeptides; D011994:Recombinant Proteins; D019063:Tenascin; C081865:tenascin X; C047981:arginyl-glycyl-aspartic acid