Characteristics of a new birnavirus associated with a warm-water fish cell line.
A warm-water fish cell line developed from blotched snakehead caudal peduncle (BSN) was found to have persistent birnavirus infection. Purified virus particles were of icosahedral shape and had 57+/-1.6 nm diameter. The BSN virus was resistant to 5-iodo-2'-deoxyuridine and induced yellowish-green cytoplasmic inclusions when stained with acridine orange. The virus was resistant to chloroform, acid and alkaline pH and heat treatment at 56 degrees C for 2 h. Purified virions had a buoyant density of 1.33 g/ml in CsCl and contained two genomic segments with molecular masses of 2.56 x 10(6) and 2.00 x 10(6) Da and four structural polypeptides of 112 (polyprotein, PP), 91 (VP1), 44 (VP2) and 37 (VP3) kDa. Reciprocal beta cross-neutralization tests incorporating four classical strains of infectious pancreatic necrosis virus (IPNV) (WB, Sp, Ab and TV-1) and the BSN virus established the complete serological distinctness of the virus from IPNV. Considering the uniqueness of the virus, the name blotched snakehead virus is proposed for this agent.
Riji John
K
K
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK1.
Richards
R H
RH
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK1.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018059:Birnaviridae / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D002460:Cell Line; D005399:Fishes / Q000821:virology*; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D015678:Viral Structural Proteins / Q000032:analysis
D000914:Antibodies, Viral; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D015678:Viral Structural Proteins