title: alpha-oxidation of fatty acids in higher plants. Identification of a pathogen-inducible oxygenase (piox) as an alpha-dioxygenase and biosynthesis of 2-hydroperoxylinolenic acid.
abstract: A pathogen-inducible oxygenase in tobacco leaves and a homologous enzyme from Arabidopsis were recently characterized (Sanz, A., Moreno, J. I., and Castresana, C. (1998) Plant Cell 10, 1523-1537). Linolenic acid incubated at 23 degrees C with preparations containing the recombinant enzymes underwent alpha-oxidation with the formation of a chain-shortened aldehyde, i.e., 8(Z),11(Z), 14(Z)-heptadecatrienal (83%), an alpha-hydroxy acid, 2(R)-hydroxy-9(Z),12(Z),15(Z)-octadecatrienoic acid (15%), and a chain-shortened fatty acid, 8(Z),11(Z),14(Z)-heptadecatrienoic acid (2%). When incubations were performed at 0 degrees C, 2(R)-hydroperoxy-9(Z),12(Z),15(Z)-octadecatrienoic acid was obtained as the main product. An intermediary role of 2(R)-hydroperoxy-9(Z), 12(Z),15(Z)-octadecatrienoic acid in alpha-oxidation was demonstrated by re-incubation experiments, in which the hydroperoxide was converted into the same alpha-oxidation products as those formed from linolenic acid. 2(R)-Hydroperoxy-9(Z),12(Z), 15(Z)-octadecatrienoic acid was chemically unstable and had a half-life time in buffer of about 30 min at 23 degrees C. Extracts of cells expressing the recombinant oxygenases accelerated breakdown of the hydroperoxide (half-life time, about 3 min at 23 degrees C), however, this was not attributable to the recombinant enzymes since the same rate of hydroperoxide degradation was observed in the presence of control cells not expressing the enzymes. No significant discrimination between enantiomers was observed in the degradation of 2(R,S)-hydroperoxy-9(Z)-octadecenoic acid in the presence of recombinant oxygenases. A previously studied system for alpha-oxidation in cucumber was re-examined using the newly developed techniques and was found to catalyze the same conversions as those observed with the recombinant enzymes, i.e. enzymatic alpha-dioxygenation of fatty acids into 2(R)-hydroperoxides and a first order, non-stereoselective degradation of hydroperoxides into alpha-oxidation products. It was concluded that the recombinant enzymes from tobacco and Arabidopsis were both alpha-dioxygenases, and that members of this new class of enzymes catalyze the first step of alpha-oxidation in plant tissue.
authors.affiliation: Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden. Mats.Hamberg@mbb.ki.se
authors.forename: M
authors.initials: M
authors.lastname: Hamberg
authors.forename: A
authors.initials: A
authors.lastname: Sanz
authors.forename: C
authors.initials: C
authors.lastname: Castresana
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000201:enzymology; D004852:Epoxy Compounds / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D007700:Kinetics; D008042:Linolenic Acids / Q000096:biosynthesis*; D008054:Lipid Peroxides / Q000096:biosynthesis*; D013058:Mass Spectrometry; D006899:Mixed Function Oxygenases / Q000378:metabolism; D008968:Molecular Conformation; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
chemical_list: D000447:Aldehydes; D004852:Epoxy Compounds; D005227:Fatty Acids; D008042:Linolenic Acids; D008054:Lipid Peroxides; D010940:Plant Proteins; C001634:fatty aldehyde; C025998:13-hydroperoxylinolenic acid; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C022333:peroxygenase; C115355:pathogen-induced oxygenase, Nicotiana tabacum