The antihyperglycaemic blend effect of traditional chinese medicine byakko-ka-ninjin-to on alloxan and diabetic KK-CA(y) mice.
The antihyperglycaemic interaction (blend effect) of component crude drugs included in a traditional Chinese prescription, Byakko-ka-ninjin-to (BN; consisted of five crude drugs, ginseng, anemarrhena,-licorice, gypsum and rice) was investigated using genetically obese diabetic KK-CA(y) mice and alloxan-diabetic mice. The water extract of ginseng, anemarrhena, licorice and gypsum when individually tested markedly lowered blood glucose levels in diabetic animals. Antihyperglycaemic effects of ginseng-anemarrhena and ginseng-licorice combinations were decreased compared with the respective individual-effects. Such decreased effects were partially reversed by adding gypsum or by substituting CaCl(2) for gypsum. The combination of D-O-ANa and glycyrrhizin, respectively the main constituents from ginseng and licorice, also demonstrated a decreased effect compared with the respective individual effects. The CaCl(2) at 0.5 mg/kg (i.p.) was sufficient to partially reverse these effects. These results indicate that antihyperglycaemic effects of BN depend on four crude drugs and Ca(2+) in the blend.
Kimura
I
I
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Nakashima
N
N
Sugihara
Y
Y
Fu-jun
C
C
Kimura
M
M
D000818:Animals; D002133:Calcium Sulfate / Q000627:therapeutic use; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000235:genetics; D004365:Drugs, Chinese Herbal; D006035:Glycyrrhiza / Q000627:therapeutic use; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008817:Mice, Mutant Strains; D005894:Panax / Q000627:therapeutic use; D008517:Phytotherapy; D010946:Plants, Medicinal; D012503:Saponins / Q000627:therapeutic use*
D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D012503:Saponins; C108060:byakko-ka-ninjin-to; D002133:Calcium Sulfate