Increased genomic damage in lymphocytes of patients before and after long-term maintenance hemodialysis therapy.
This study investigates spontaneous genomic damage in peripheral lymphocytes of 19 patients with severe end-stage renal disease not enrolled onto a maintenance hemodialysis (MHD) program (creatinine level, 5.4 to 10.5 mg/dL) and 16 long-term MHD patients (111 to 282 months on MHD) and the possible association of genomic damage with the degree of renal insufficiency and duration of MHD. Genomic damage was assessed by evaluating the numbers of micronuclei (MN), which are cytoplasmic DNA-containing structures. The average number of MN in the control group of 23 healthy subjects was 15.3 +/- 4.7 MN/1,000 binucleate (BN) cells. The MN frequencies in the long-term MHD group were significantly greater (44.3 +/- 13.7 MN/1,000 BN) than the control frequencies. A significant increase in MN frequencies (28.2 +/- 9.4 MN/1,000 BN) was also seen in patients with advanced renal failure. The major findings of the study, high MN frequencies in long-term hemodialysis and advanced chronic renal failure patients, may result from decreased DNA repair previously shown and may contribute to the increased cancer incidence in these patients.
Stopper
H
H
Institut für Toxikologie der, Universität Würzburg, Würzburg, Germany.
Meysen
T
T
Böckenförde
A
A
Bahner
U
U
Heidland
A
A
Vamvakas
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations / Q000235:genetics; D004249:DNA Damage / Q000235:genetics*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics / Q000628:therapy*; D007677:Kidney Function Tests; D008134:Long-Term Care; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D006435:Renal Dialysis; D018570:Risk Assessment