Thermal inactivation kinetics of Bacillus stearothermophilus spores using a linear temperature program.
A systematic study of the inactivation kinetics of Bacillus stearothermophilus spores was carried out in nonisothermic heating conditions using a linear temperature increase program and analyzing the experimental data by means of a one-step nonlinear regression. The D and z values estimated are close to those obtained in isothermic conditions and estimated by using a two-step model, first D values are calculated, and then in the second step a z value is deduced (D(121 degrees C) = 3.08 and 4.38 min, respectively, and z = 7 and 7.9 degrees C, respectively). No convergence problems were observed when using the one-step nonlinear regression proposed. The results indicated that the methodology applied in this study can be used to obtain kinetic data for bacterial spores, which could mean a significant reduction in the amount of experimental work employed to generate these data.
Leontidis
S
S
Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Valencia, Spain.
Fernández
A
A
Rodrigo
C
C
Fernández
P S
PS
Magraner
L
L
Martínez
A
A
D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D001411:Geobacillus stearothermophilus / Q000502:physiology*; D006358:Hot Temperature; D013171:Spores, Bacterial / Q000502:physiology; D013696:Temperature