title: Endoscopic biopsy pathology of Helicobacter pylori gastritis. Comparison of bacterial detection by immunohistochemistry and Genta stain.
abstract: OBJECTIVE
  To describe the endoscopic biopsy pathology of Helicobacter pylori gastritis, compare bacterial detection by immunohistochemistry using a specific antibody with the Genta stain, and to compare the relative costs of the 2 techniques.
  
  
  METHODS
  One hundred cases of gastritis identified as positive for H pylori by Genta stain and 100 cases considered negative by the same technique were stained using an anti-H pylori-specific polyclonal antibody. Laboratory reagent and labor costs for the 2 methods were compared.
  
  
  RESULTS
  Chronic active gastritis with lymphoid follicles was significantly associated with H pylori infection (P <.0001). The immunohistochemical method had a sensitivity of 97% and a specificity of 98% compared with the Genta stain, with strong agreement for grading density of organisms (kappa = 0.85; P <.001). Reagent costs were similar for both methods, but immunohistochemistry using an autoimmunostainer required less dedicated technical time and hence was less expensive than the Genta stain.
  
  
  CONCLUSIONS
  Immunohistochemistry using a specific antibody is an accurate and cost-effective method for H pylori detection in gastric biopsies.
authors.affiliation: Department of Pathology, Baystate Medical Center, Springfield, MA 01199, USA.
authors.forename: M
authors.initials: M
authors.lastname: Toulaymat
authors.forename: S
authors.initials: S
authors.lastname: Marconi
authors.forename: J
authors.initials: J
authors.lastname: Garb
authors.forename: C
authors.initials: C
authors.lastname: Otis
authors.forename: S
authors.initials: S
authors.lastname: Nash
mesh_terms: D001706:Biopsy; D004396:Coloring Agents; D005756:Gastritis / Q000382:microbiology* / Q000473:pathology*; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D007150:Immunohistochemistry / Q000191:economics; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000191:economics
chemical_list: D004396:Coloring Agents