title: Additive effects of beta chain mutations in low oxygen affinity hemoglobin betaF41Y,K66T.
abstract: In order to decrease significantly the oxygen affinity of human hemoglobin, we have associated the mutation betaF41Y with another point mutation also known to decrease the oxygen affinity of Hb. We have synthesized a recombinant Hb (rHb) with two mutations in the beta chains: rHb betaF41Y,K66T. In the absence of 2, 3-diphosphoglycerate, additive effects of the mutations are evident, since the doubly mutated Hb exhibits a larger decrease in oxygen affinity than for the individual single mutations. In the presence of 2,3-diphosphoglycerate, the second mutation did not significantly increase the P(50) value relative to the single mutations. However, the kinetics of CO binding still indicate combined effects on the allosteric equilibrium, as evidenced by more of the slow bimolecular phase characteristic of binding to the deoxy conformation. Dimer-tetramer equilibrium studies indicate an increase in stability of the mutants relative to rHb A; the double mutant rHb betaF41Y, K66T at pH 7.5 showed a K(4,2) value of 0.26 microM. Despite the lower oxygen affinity, the single mutant betaF41Y and double mutant betaF41Y,K66T show only a moderate increase of 20% in the autoxidation rate. These mutations are thus of interest in developing a Hb-based blood substitute.
authors.affiliation: INSERM, Unité 473, 84 rue du Général Leclerc, 94276 Le Kremlin-Bicêtre Cedex, France. baudin@kb.inserm.fr
authors.forename: V
authors.initials: V
authors.lastname: Baudin-Creuza
authors.forename: C
authors.initials: C
authors.lastname: Vasseur-Godbillon
authors.forename: N
authors.initials: N
authors.lastname: Griffon
authors.forename: J
authors.initials: J
authors.lastname: Kister
authors.forename: L
authors.initials: L
authors.lastname: Kiger
authors.forename: C
authors.initials: C
authors.lastname: Poyart
authors.forename: M C
authors.initials: MC
authors.lastname: Marden
authors.forename: J
authors.initials: J
authors.lastname: Pagnier
mesh_terms: D004926:Escherichia coli; D005914:Globins / Q000235:genetics; D006454:Hemoglobins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D010100:Oxygen / Q000378:metabolism*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D011994:Recombinant Proteins; D005914:Globins; D010100:Oxygen