title: Surgically induced degeneration and regeneration of the choriocapillaris in rabbit.
abstract: BACKGROUND
  Retinal pigment epithelium (RPE) and choriocapillaris play an important role in maintaining the outer retina. Clinical and experimental studies have shown that there is a close relationship between them. To examine the relationship between RPE and choriocapillaris we developed an animal model in which degeneration and regeneration of the choriocapillaris can be created easily and reproducibly.
  
  
  METHODS
  Using pigmented rabbits a retinal detachment (about 7 disk diameters) was created in the eye and the detached retina was surgically removed. A half area of the exposed RPE was mechanically debrided using a silicone brush. The other half area remained untouched. The eyes were morphologically examined at 3, 7, and 14 days and 4 and 8 weeks after the surgery using scanning or transmission electron microscopy. Changes in the choriocapillaris after the RPE debridement were also evaluated by corrosion vascular casts.
  
  
  RESULTS
  The debrided area was resurfaced with elongated RPE in 7 days after surgery. The replaced RPE was dedifferentiated but it gradually recovered cell polarity by 4 weeks after surgery. The choriocapillaris beneath the debrided area underwent degeneration; however, it regenerated by 4 weeks after surgery, corresponding to the timing of the morphologic recovery of the replaced RPE.
  
  
  CONCLUSIONS
  This animal model of surgically induced degeneration and regeneration of the choriocapillaris may be useful to clarify the relationship between RPE and choriocapillaris and to study potential treatments for choroidal vascular diseases.
authors.affiliation: Wilmer Ophthalmological Institute, The Johns Hopkins University, School of Medicine, Baltimore, MD 21287, USA.
authors.forename: A
authors.initials: A
authors.lastname: Hayashi
authors.forename: A B
authors.initials: AB
authors.lastname: Majji
authors.forename: S
authors.initials: S
authors.lastname: Fujioka
authors.forename: H C
authors.initials: HC
authors.lastname: Kim
authors.forename: I
authors.initials: I
authors.lastname: Fukushima
authors.forename: E
authors.initials: E
authors.lastname: de Juan
mesh_terms: D000818:Animals; D002196:Capillaries / Q000502:physiology* / Q000601:surgery / Q000648:ultrastructure; D002829:Choroid / Q000098:blood supply* / Q000601:surgery; D016319:Corrosion Casting; D005451:Fluorescein Angiography; D005500:Follow-Up Studies; D005654:Fundus Oculi; D008297:Male; D008855:Microscopy, Electron, Scanning; D010857:Pigment Epithelium of Eye / Q000502:physiology / Q000601:surgery* / Q000648:ultrastructure; D011817:Rabbits; D014945:Wound Healing