title: GABAergic system in the developing mammalian retina: dual sources of GABA at early stages of postnatal development.
abstract: In the present work, we have characterized the maturation of the GABAergic system in mammalian retina. Immunoreactivity for GABA, GAD (glutamic acid decarboxylase, EC 4.1.1.15) -65 and -67 in the adult rat retina was localized in cells in the inner nuclear and ganglion cell layers. This pattern was established around postnatal day 8 and included transient GABA and GAD-67 expression in horizontal cells. GAD activity was very low at P1 and P4, increasing after P8, reaching maximal activity by P21 and decreasing to attain adult values by P30. GABA content was approximately constant from P1 to P13, increasing thereafter to reach adult levels. GAD protein content increased progressively with postnatal development and the two isoforms could be distinguished at P8. The disparity between retinal GABA content vs. presence and activity of the synthesizing enzyme, led us to investigate the alternative pathway for GABA synthesis that utilizes putrescine as a substrate. Highest levels of ornithine decarboxylase activity (the limiting step for putrescine synthesis) were found between P1 and P4, decreasing to very low levels after P13. The same pattern was observed for putrescine content in the retina. Highest amounts were found at P1, that decreased and remained constant after P13. Additionally, approximately 40% of tritiated putrescine incorporated by P1, P4 and adult retinas was converted into GABA. Our results suggest the existence of two different sources of GABA in mammalian retina, one that uses glutamate as a precursor and predominates in the mature nervous system and another that utilizes putrescine and is present transiently at early developmental stages.
authors.affiliation: Laboratório de Neurobiologia da Retina, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Brazil. yamasaki@ucy.ac.cy
authors.forename: E N
authors.initials: EN
authors.lastname: Yamasaki
authors.forename: V D
authors.initials: VD
authors.lastname: Barbosa
authors.forename: F G
authors.initials: FG
authors.lastname: De Mello
authors.forename: J N
authors.initials: JN
authors.lastname: Hokoc
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005968:Glutamate Decarboxylase / Q000032:analysis*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D012160:Retina / Q000737:chemistry* / Q000254:growth & development; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D007527:Isoenzymes; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; D009955:Ornithine Decarboxylase; D011700:Putrescine