title: A search for a mutation in the tumour necrosis factor-alpha gene in narcolepsy.
abstract: The discovery of almost 100% association of narcolepsy with human leukocyte antigens (HLA) DR2 antigen prompted molecular biological research of this disorder. In the HLA class II gene cluster, the gene for tumour necrosis factor-alpha (TNF-alpha), which plays a role in the regulation of normal human sleep, is located. The present study searched for a mutation in the TNF-alpha gene by single-strand conformation polymorphism analysis (SSCP) in patients with narcolepsy. No mutation was detected in exons and introns of the TNF-alpha gene by SSCP and sequencing.
authors.affiliation: Department of Neuropsychiatry, Faculty of Medicine, University of Tokyo, Japan. tadafumitky@umin.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.forename: S
authors.initials: S
authors.lastname: Kuwata
authors.forename: T
authors.initials: T
authors.lastname: Juji
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
authors.forename: N
authors.initials: N
authors.lastname: Kato
mesh_terms: D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D015802:HLA-DR2 Antigen / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009290:Narcolepsy / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D015802:HLA-DR2 Antigen; D014409:Tumor Necrosis Factor-alpha