Use of a translationally coupled reporter gene to eliminate nonsense mutations in a random mutagenesis study.
Wang
J
J
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.
Chen
R
R
Julin
D A
DA
D001483:Base Sequence; D018389:Codon, Nonsense / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D007613:Kanamycin Resistance / Q000235:genetics; D016296:Mutagenesis; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics
D018389:Codon, Nonsense; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D011994:Recombinant Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V