Knock-out of the cyaY gene in Escherichia coli does not affect cellular iron content and sensitivity to oxidants.
Friedreich ataxia is a recessively inherited neurodegenerative disease caused by deficiency of a highly conserved mitochondrial protein, frataxin. Frataxin deficiency results in mitochondrial iron accumulation and oxidative stress. Frataxin shows homology with the CyaY proteins of gamma-purple bacteria, whose function is unknown. We knocked out the CyaY gene in Escherichia coli MM383 by homologous recombination and we generated an E. coli MM383 strain overexpressing CyaY. Bacterial growth, iron content and survival after exposure to H2O2 did not differ among these strains, suggesting that, despite structural similarities, cyaY proteins in bacteria may have a different function from frataxin homologues in mitochondria.
Li
D S
DS
Research Center, Centre Hospitalier de l'Université de Montréal, Hôpital Notre-Dame, Montreal, Que., Canada.
Ohshima
K
K
Jiralerspong
S
S
Bojanowski
M W
MW
Pandolfo
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division / Q000235:genetics; D017124:Conserved Sequence; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D011995:Recombination, Genetic; D000096923:Frataxin
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D007501:Iron; D033862:Iron-Binding Proteins; D016877:Oxidants; D017853:Phosphotransferases (Alcohol Group Acceptor); D000096923:Frataxin; C120906:CyaY protein, E coli