title: Perkinsus marinus extracellular protease modulates survival of Vibrio vulnificus in Eastern oyster (Crassostrea virginica) hemocytes.
abstract: The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced by P. marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues.
authors.affiliation: Divisions of Microbiological Studies, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204, USA. BTall@bangate.fda.gov
authors.forename: B D
authors.initials: BD
authors.lastname: Tall
authors.forename: J F
authors.initials: JF
authors.lastname: La Peyre
authors.forename: J W
authors.initials: JW
authors.lastname: Bier
authors.forename: M D
authors.initials: MD
authors.lastname: Miliotis
authors.forename: D E
authors.initials: DE
authors.lastname: Hanes
authors.forename: M H
authors.initials: MH
authors.lastname: Kothary
authors.forename: D B
authors.initials: DB
authors.lastname: Shah
authors.forename: M
authors.initials: M
authors.lastname: Faisal
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000201:enzymology*; D015169:Colony Count, Microbial; D006434:Hemocytes / Q000382:microbiology*; D010125:Ostreidae / Q000378:metabolism / Q000382:microbiology* / Q000469:parasitology; D012697:Serine Endopeptidases / Q000378:metabolism*; D014733:Vibrio / Q000254:growth & development*
chemical_list: D012697:Serine Endopeptidases