Synthesis of new photocross-linking 5-C-base-substituted UTP analogs and their application in highly selective affinity labelling of the tick-borne encephalitis virus RNA replicase proteins.
A new photocross-linking 5-C-base-substituted UTP analogs, carrying 4-azidoperfluorobenzoyl and 4-azidoaniline residues were synthesized. Two flavivirus proteins NS5 and NS3 are shown to be labelled after RNA synthesis in the presence of the analogs, irradiation (lambda > 300 nm) and subsequent [alpha-32P]NTP incorporation.
Morozova
O V
OV
Institute of Bioorganic Chemistry, Novosibirsk, Russia.
Kolpashchikov
D M
DM
Ivanova
T M
TM
Godovikova
T S
TS
D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D004669:Encephalitis Viruses, Tick-Borne / Q000201:enzymology*; D010777:Photochemistry; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D013057:Spectrum Analysis; D013552:Swine; D014544:Uridine Triphosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry
D000345:Affinity Labels; D012324:RNA-Dependent RNA Polymerase; D014544:Uridine Triphosphate