Primary cultures of heart cells from the scallop Pecten maximus (mollusca-bivalvia).
Primary cultures of Pecten maximus heart cells, isolated by an enzymatic procedure, were routinely obtained with a high level of reproducibility in a simple medium based on sterile seawater. Cells attached to the plastic substratum without the need to add a special factor. The number of adhering cells gradually increased with the time of culture. Two types of adhering cells were observed: epitheliallike cells and fibroblastlike cells, which were more numerous. The latter cells were identified as myocytes by electron microscopy and immunofluorescent staining. Results obtained by autoradiography, after incorporation of [14C]leucine, [3H]thymidine, and [14C]acetate, confirmed functional activity of the cells. These cultures were maintained viable in vitro during at least 1 mo.
Le Marrec-Croq
F
F
Unité de Culture cellulaire, Université de Bretagne Occidentale, Brest, France.
Glaise
D
D
Guguen-Guillouzo
C
C
Chesne
C
C
Guillouzo
A
A
Boulo
V
V
Dorange
G
G
D000818:Animals; D001345:Autoradiography; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008974:Mollusca / Q000166:cytology*; D009206:Myocardium / Q000166:cytology; D012623:Seawater; D013997:Time Factors
D003470:Culture Media; D004247:DNA