title: On-line techniques for perfluorochemical vapor sampling and measurement.
abstract: The authors developed a compact gas sampling and perfluorochemical (PFC) measuring system for use in total and partial liquid ventilation systems, based on a precision two-thermistor thermal detector (TD). They describe the sensitivity and linearity of their on-line method for PFC analysis of expired gases and show how it may be used in partial liquid ventilation studies for determining PFC saturation and loss. Gas is sampled for a short time from a breathing circuit through a heated tube at a selectable point in the breathing cycle. Inspiration is sensed by a pressure transducer. The sample of gas is pulled into the heated (48 degrees C) thermistor chamber by suction and held there while the cooling effect of the vapor changes the thermistor temperature. Dry air in another chamber affects a second thermistor, and the difference of these responses is amplified. The raw signal is corrected for the effects of varying O2 levels by a fuel cell. This signal is sampled and held and displayed on a front panel display. Calibration is performed in percentage saturation at 37 degrees C using the PFC in use at that temperature, or another standard such as O2. In-vitro testing showed a linear response in the thermal detector device (R2 = 0.99) over the range of vapor pressures tested (0-14) mmHg) and was reproducible to within 3%. When electronically corrected for changes in O2 concentration, there was less than a 2% change in PFC saturation. The TD responses to CO2 (R2 = 0.99) and water vapor (R2 = 1.0) were linear and approximately equal and opposite over the normal operating ranges of expired gases. In-vivo results in rabbits showed a significant (R2 = 0.73; p < 0.01) correlation between the auto-sampler and manual collection modes for determination of PFC in expired gas.
authors.affiliation: Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, USA. cphilips@nimbus.ocis.temple.edu
authors.forename: C M
authors.initials: CM
authors.lastname: Philips
authors.forename: C
authors.initials: C
authors.lastname: Weis
authors.forename: W W
authors.initials: WW
authors.lastname: Fox
authors.forename: M R
authors.initials: MR
authors.lastname: Wolfson
authors.forename: T H
authors.initials: TH
authors.lastname: Shaffer
mesh_terms: D000818:Animals; D001944:Breath Tests / Q000295:instrumentation*; D003626:Data Display; D004655:Emulsions / Q000008:administration & dosage* / Q000032:analysis; D004867:Equipment Design; D005466:Fluorocarbons / Q000008:administration & dosage* / Q000032:analysis*; D005740:Gases / Q000032:analysis*; D006801:Humans; D006842:Hydrocarbons, Brominated; D008991:Monitoring, Physiologic / Q000295:instrumentation; D011312:Pressure; D011817:Rabbits; D015203:Reproducibility of Results; D012121:Respiration, Artificial / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D013696:Temperature; D014159:Transducers
chemical_list: D004655:Emulsions; D005466:Fluorocarbons; D005740:Gases; D006842:Hydrocarbons, Brominated; C003072:perflubron