Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis.
Here we report the functional importance of profilin in various actin-mediated morphological changes using H119E mutant profilin I, which is deficient only in actin binding. In the case of actin-protrusive structures from the plasma membrane, H119E-profilin was shown to suppress the formation of Cdc42-induced actin microspikes and Rac-induced membrane ruffles. Conversely, Rho-induced stress fiber formation seemed to occur independently of H119E-profilin introduction. Furthermore, H119E-profilin blocked cleavage furrow ingression and subsequent adhesion to the substratum during cell division, a process in which actin plays indispensable roles.
Suetsugu
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S
Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Miki
H
H
Takenawa
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T
D016475:3T3 Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D003285:Contractile Proteins; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism* / Q000502:physiology*; D009154:Mutation; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051304:Profilins; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins
D000199:Actins; D018797:Cell Cycle Proteins; D003285:Contractile Proteins; D008246:Lysophospholipids; D008840:Microfilament Proteins; C495738:PFN1 protein, human; C495739:Pfn1 protein, mouse; D010982:Platelet-Derived Growth Factor; D051304:Profilins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins