title: Random amplification of polymorphic DNA fingerprinting of Trypansoma evansi.
abstract: The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eight arbitrarily selected 10-base primers. Informative band patterns were obtained for all isolates analysed. Depending upon the T. evansi isolate primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (random amplified polymorphic DNA) profiles.
authors.affiliation: National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar.
authors.forename: S H
authors.initials: SH
authors.lastname: Basagoudanavar
authors.forename: J R
authors.initials: JR
authors.lastname: Rao
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.forename: G
authors.initials: G
authors.lastname: Butchaiah
mesh_terms: D000818:Animals; D000866:Antelopes / Q000469:parasitology; D002162:Camelus / Q000469:parasitology; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D006736:Horses / Q000469:parasitology; D007194:India; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan