Interleukin-12, interferon-gamma and interleukin-4 gene expression in cats infected with Toxoplasma gondii.
Interleukin-12, Interferon-gamma and Interleukin-4 mRNA levels in cells of the spleen and mesenteric lymph nodes of cats following primary and secondary infection with Toxoplasma gondii were examined by a semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) method. Expression of Interleukin-12p40 mRNA and Interferon-gamma mRNA was observed after primary and secondary oral infection with Toxoplasma gondii. In contrast, no expression of IL-4 mRNA in the spleen and little expression in the mesenteric lymph nodes were observed after primary infection when the cats shed oocysts, however, the expression of IL-4 mRNA observed in the cats after secondary inoculation.
Koyama
T
T
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Omata
Y
Y
Maki
Y
Y
Toyoda
Y
Y
Saito
A
A
D000818:Animals; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D005786:Gene Expression Regulation / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics*; D018664:Interleukin-12 / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008198:Lymph Nodes / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014122:Toxoplasma; D014124:Toxoplasmosis, Animal / Q000235:genetics / Q000276:immunology*; D014158:Transcription, Genetic
D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma