Inhibition of infection of incoming HIV-1 virus by RNA-cleaving DNA enzyme.
Nine different DNA enzymes (DzV3-n, n=1-9) targeting the V3 loop region of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors. The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient, stable DNAzyme against a functionally important region of HIV-1 was identified, and it may be useful for prevention of HIV-1 infection.
Zhang
X
X
Division of Allergy and Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Japan.
Xu
Y
Y
Ling
H
H
Hattori
T
T
D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001483:Base Sequence; D002384:Catalysis; D002460:Cell Line; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000494:pharmacology*; D004279:DNA, Viral / Q000138:chemical synthesis / Q000494:pharmacology*; D004795:Enzyme Stability; D015686:Gene Products, env / Q000378:metabolism; D015658:HIV Infections / Q000201:enzymology / Q000517:prevention & control / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014779:Virus Replication
D000998:Antiviral Agents; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015686:Gene Products, env; D010446:Peptide Fragments; D012367:RNA, Viral