Cultured rabbit corneal epithelium elicits levofloxacin absorption and secretion.
Abstract Evidence for carrier-mediated transport of levofloxacin in the isolated rabbit cornea has been found. However, it is not known whether this mechanism is located in the epithelium or the endothelium. To resolve this question, we have measured the kinetics of levofloxacin uptake in primary cultures of rabbit corneal epithelial cells. The results indicate that levofloxacin accumulation was time dependent and a steady state was reached after 30 min. Maximal uptake occurred from a solution whose pH was 6.5. The uptake process was stereoselective and concentration dependent. In addition to the uptake, secretion of levofloxacin also occurred. These results indicate that the corneal epithelium is the site of levofloxacin transport mechanisms, mediating both absorption and secretion.
Kawazu
K
K
Santen Pharmaceutical Co. Ltd, Nara Research and Development Center, Ophthalmic Research Division, Japan. kawazu@santen.co.jp
Midori
Y
Y
Ota
A
A
D000042:Absorption; D000818:Animals; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D019573:Epithelium, Corneal / Q000166:cytology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D064704:Levofloxacin; D015242:Ofloxacin / Q000737:chemistry / Q000493:pharmacokinetics*; D011817:Rabbits; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors
D000890:Anti-Infective Agents; D064704:Levofloxacin; D015242:Ofloxacin