[Spinal segment distribution of neural innervation related houhai acupoint--studied by CB-HRP tracing method focused on observation of the dendrites of spinal motor neurons].
The spinal segment distribution of neural innervation related Houhai acupoint in rats has been investigted by using horseradish peroxidase conjugated cholera toxin B subunit (CB-HRP) tracing technique. Spinal motoneurons (Mn) in L6-S1 were labeled retrogradely by CB-HRP, and their features have been characterized: 1) their marginal plexus of the dendrites radially projected to the lateral and/or anterior funiculus and their distal portions forming subpial dendritic plexus; 2) the medial dendrites ran along the ventral margin of the gray commissure and their distal portions surrounded the central canal' forming a subependymal dendritic plexus. Both of them are Golgi-phobic. Some distal branches of dendrites penetrated to the contralateral gray matter. The dendritic arbors of the labeled motoneurons in the group of electro-acupuncture or group of acupoint-inoculation with sheep red blood cells contracted in comparison with that in the normal control group. The primary sensory transganglionic labeled fibers and preterminal fibers revealed by CB-HRP in the dorsal horn and dorsal commissural nucleus of the spinal cord (L5-S2) converged and overlaped with the dendrites of retrogradely labeled motoneurons. The results indicated there is a reflect circuit of afferent-integration-efferent neuro-network in the spinal cord related Houhai acupoint. The Mns retrogradely labeled by CB-HRP in the L6-S1 segments might be involved in Houhai acupuncture.
Tan
H
H
Institute of Basic Medical Sciences, CAMS, Beijing.
Shi
G
G
Lin
J
J
Liu
K
K
Zuo
Z
Z
D015669:Acupuncture Points; D000818:Animals; D002772:Cholera Toxin / Q000378:metabolism; D003712:Dendrites / Q000502:physiology; D015671:Electroacupuncture; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009434:Neural Pathways; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000502:physiology
C052635:cholera toxin, B subunit-horseradish peroxidase; D002772:Cholera Toxin; D006735:Horseradish Peroxidase