Effect of C1-inhibitor in a rat model of necrotizing enterocolitis.
Our aim was to investigate the effect of C1-inhibitor (C1-INH) in a rat model of necrotizing enterocolitis (NEC). Twenty-four anesthetized and artificially ventilated rats received 0.1 mg/kg endotoxin. Fifteen minutes later, the animals in the study group (n = 12) were treated with 200 IU/kg C1-INH whereas the control animals (n = 12) received normal saline. In all animals, FiO(2) was reduced after 90 min from 0.21 to 0.05 and ventilation continued until 180 min or death. All animals developed shock symptoms. Drop in mean arterial blood pressure was more pronounced and survival time was shorter in the control group. Whereas the C1-INH activity increased in the study group, it decreased in the control group. The extent of macroscopic intestinal lesions did not differ between the groups. In conclusion, C1-INH did not prevent shock, but mitigated and delayed its course.
Sonntag
J
J
Department of Neonatology, Charité, Campus Virchow Hospital, Humboldt University, Berlin, Germany.
Wagner
M H
MH
Liu
G
G
Vogel
M
M
Walka
M M
MM
Maier
R F
RF
D000818:Animals; D001794:Blood Pressure; D003174:Complement C1 Inactivator Proteins / Q000627:therapeutic use*; D003171:Complement Pathway, Classical; D004195:Disease Models, Animal; D020345:Enterocolitis, Necrotizing / Q000139:chemically induced / Q000150:complications*; D006339:Heart Rate; D008070:Lipopolysaccharides; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012769:Shock / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology
D003174:Complement C1 Inactivator Proteins; D008070:Lipopolysaccharides