Short-chain fatty acids stimulate mucosal cell proliferation in the closed human rectum after Hartmann's procedure.
Hartmann's procedure in humans results in a closed rectum deprived of its natural short-chain fatty acid source. This induces atrophy of the entire rectal wall. Ki-67 is a monoclonal antibody directed towards proteins in the cell nucleus that are present only during cell proliferation. This study investigated the effects of short-chain fatty acids on mucosal cell proliferation in the human rectum after Hartmann's procedure by means of Ki-67. Eight patients in whom Hartmann's procedure was performed were treated with placebo and then short-chain fatty acids for 14 days. Biopsies specimens were taken from the rectum before and after treatment; these were prepared with Ki-67 and labeling index was determined. The treatment was found significantly to increase proliferative activity in the rectal mucosa (P<0.01); the increase was principally in the middle (P<0.01) and upper crypt (P<0.05) compartments.
Mortensen
F V
FV
Department of Gastrointestinal Surgery L, Aarhus University Hospital, Tage Hansensgade 2, DK-8000 Aarhus C, Denmark, F.V.Mortensen@Dadlnet.dk
Langkilde
N C
NC
Joergensen
J C
JC
Hessov
I
I
D000328:Adult; D000714:Anastomosis, Surgical; D002455:Cell Division; D003082:Colectomy; D013505:Digestive System Surgical Procedures / Q000379:methods; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000276:immunology; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012007:Rectum / Q000502:physiology / Q000601:surgery*
D005232:Fatty Acids, Volatile; D019394:Ki-67 Antigen