Expression of N-acetyl galactosaminylated and sialylated glycans by metastases arising from primary breast cancer.
This study examines the Helix pomatia lectin (HPA) binding characteristics of metastases arising from primary breast cancer, and compares HPA binding patterns with binding of Dolichos biflorus lectin (DBA), Limax flavus lectin (LFA), and a monoclonal antibody against the Tn epitope. Of 81 blocks of metastases in a range of tissues, taken at autopsy from 46 individuals, 79% were HPA positive. No site specificity with regard to HPA binding was observed. Both HPA-positive and -negative tumour deposits were seen within a single individual. HPA-positive tumours were commonly negative for binding of sialic acid specific lectin LFA (86% were negative). Binding patterns of alpha-GalNAc specific HPA and DBA, and a monoclonal antibody against Tn epitope (GalNAc-O-Ser/Thr) were markedly different.
Brooks
S A
SA
School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK. sbrooks@brookes.ac.uk
Leathem
A J
AJ
D000116:Acetylgalactosamine / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D000939:Epitopes / Q000378:metabolism; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D009362:Neoplasm Metastasis; D037121:Plant Lectins; D011134:Polysaccharides / Q000032:analysis*; D011975:Receptors, Mitogen / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D000939:Epitopes; C038095:Helix lectin; D037102:Lectins; C042962:Limax flavus agglutinin; D037121:Plant Lectins; D011134:Polysaccharides; D011975:Receptors, Mitogen; C040286:Tn antigen; C036554:dolichos biflorus agglutinin; C042967:helix lectin receptor; C055491:sialosyl-Tn antigen; D019158:N-Acetylneuraminic Acid; D000116:Acetylgalactosamine