Structural studies on the polar glycoinositol phospholipids of Trypanosoma (Schizotrypanum) dionisii from bats.
The polar glycoinositol phospholipids (GIPLs) of a Trypanosoma species that belongs to the Schizotrypanum subgenus were purified by reversed-phase and normal-phase liquid chromatography and analysed by negative-ion mode electrospray-mass spectrometry (ES-MS). The phosphatidylinositol moieties were released by nitrous acid deamination and identified as ceramide- and alkylacylglycerol-containing species. The structures of the GIPLs were determined using chemical treatments, sequential exoglycosidase digestions and positive-ion mode ES-MS-MS. All of the GIPLs were based on the same Man alpha1-2Man alpha1-2Man alpha1-6Man alpha1-4(NH2-CH2CH2-HPO3-)GlcN-PI core with single terminal Galf residue substitutions either on the terminal nonreducing Man or on the second alphaMan residue from the inositol and with either ethanolamine phosphate or 2-aminoethylphosphonate on the third alphaMan residue from the inositol. The T. (S.) dionisii GIPLs are compared with those of T. (S.) cruzi, a closely related species of the Schizotrypanum subgenus.
Branquinha
M H
MH
Departamento de Microbiologia Geral, Instituto de Microbiologia Professor Paulo de Góes, UFRJ, Rio de Janeiro, Brazil.
Vermelho
A B
AB
Almeida
I C
IC
Mehlert
A
A
Ferguson
M A
MA
D000818:Animals; D002240:Carbohydrate Sequence; D002685:Chiroptera / Q000469:parasitology*; D002845:Chromatography / Q000379:methods; D017261:Glycosylphosphatidylinositols / Q000737:chemistry* / Q000302:isolation & purification; D008745:Methylation; D008969:Molecular Sequence Data; D013057:Spectrum Analysis / Q000379:methods; D014345:Trypanosoma / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000737:chemistry*
D017261:Glycosylphosphatidylinositols