title: Mapping studies and genetic analysis of transfer genes of the multi-resistant IncHI2 plasmid, R478.
abstract: A bank of transfer-defective Tn7 insertion mutants of the multi-resistant IncHI2 megaplasmid, R478, was generated. Complementation analysis of these mutations identified a large 144-kb transfer-associated region of R478. A 6.8-kb segment from the transfer region was sequenced. The precise locations of Tn7 insertion within four distinct R478::Tn7 transfer-defective mutants were mapped and each insertion was found to disrupt a specific open reading frame. These transfer-associated determinants of R478 were designated htdB (H transfer determinant), htdD, htdT and htdC. Both htdB and htdC encoded amino acid sequences that showed a low homology with pilus biosynthetic proteins encoded by the F plasmid.
authors.affiliation: Department of Microbiology, National University of Ireland, Galway, Ireland.
authors.forename: D T
authors.initials: DT
authors.lastname: Page
authors.forename: K F
authors.initials: KF
authors.lastname: Whelan
authors.forename: E
authors.initials: E
authors.lastname: Colleran
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D036781:Fimbriae Proteins; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011815:R Factors / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C122117:HtdB protein, Serratia marcescens; C122118:HtdC protein, Serratia marcescens; C074790:traC protein, Plasmid F; D036781:Fimbriae Proteins