[Down-regulation effect of HIM82 on respiratory burst of neutrophil].
OBJECTIVE
The early control of respiratory burst of Neutrophil (PMN) was studied by using McAb HIM82.


METHODS
PMNs were isolated from healthy volunteers. Superoxide anion, Hydrogen peroxide were measured in the presence of HIM82 FMLP. Sustance P and the NEP activities was measured in the presence of HIM82 alone.


RESULTS
The amount of O2.- and H2O2 produced in experimental group (by combining with HIM82) are (44.00 +/- 8.9)% (n = 8, P < 0.01) and (65.16 +/- 15.41)% (n = 8, P < 0.01) of control group respectively. The activity of NEP inactivated by PMA (without HIM82) is (43.29 +/- 9.41)% (n = 8, P < 0.05) and the activity is (66.48 +/- 15.53)% (n = 8, P < 0.05) in PMA-inactivated group with HIM82.


CONCLUSIONS
The result mentioned above showed that the HIM82 monoclonal antibody exerts obvious down regulation on respiration burst induced by PMA and antagniation on inactivity of NEP. We under stand that this McAb has important regulation effect on function of PMN, it shows that the specific combination with corresponding antigen on membrane is essential for function of PMN especially for mechanism of respiratory burst of activated PMN.
Jiang
X
X
Institute of Hematology, PUMC, Tianjing.
Fa
X
X
Lu
S
S
Shen
D
D
D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015260:Neprilysin / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst; D013755:Tetradecanoylphorbol Acetate
D000911:Antibodies, Monoclonal; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D015260:Neprilysin; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen