Electroacupuncture reduces rat's neuronal ischemic injury and enhances the expression of basic fibroblast growth factor.
The present study was designed to investigate whether the Electroacupuncture (EA) is beneficial to extenuate cerebral injuries following transient Middle Cerebral Artery Occlusion (MCAO), as well as to observe the effect of EA on expression of Basic Fibroblast Growth Factor (bFGF) -like Immunoreactivity (IR) in rat brains. The results indicate that gross neuronal damages include infarction, swelling and neuron loss, accompanied by increased bFGF-like IR expression following MCAO. In peri-infarct striatum, bFGF-like IR was mainly located in astrocytes except some neurons also showed an upregulation of the IR; in frontoparietal cortex, strong induction of bFGF-like IR was mostly seen in neurons. Both the EA applied during ischemia and reperfusion could evidently alleviate cerebral lesion extent, notably upregulate the expression of bFGF-like IR in striatum and cortex, but there was no significant difference between the effects of EA applied during ischemia and reperfusion, except EA applied during reperfusion seems to be more effective in reducing the cerebral swelling. The results implied that, in striatum, astrocytes might play an important role in the protection of neuron via the expression of bFGF; whereas in cortex, neurons may exert autoprotection through secreting bFGF themselves. One possible protective effect of EA lies in regulating the endogenous expression of bFGF.
Yang
W O
WO
National Laboratory of Medical Neurobiology, Institute of Acupuncture Research, Shanghai Medical University, P.R. China.
Huang
Y L
YL
Da
C D
CD
Cheng
J S
JS
D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D003342:Corpus Striatum / Q000473:pathology; D004195:Disease Models, Animal; D015671:Electroacupuncture; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000096:biosynthesis*; D005625:Frontal Lobe / Q000473:pathology; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000473:pathology; D010296:Parietal Lobe / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
D016222:Fibroblast Growth Factor 2