title: Metabolic and functional effects of low-potassium cardioplegic solutions for long-term heart preservation.
abstract: Cardioplegic solutions used to arrest the heart during open heart surgery and cardiac transplantation are based on potassium as a cardioplegic agent in a concentration range of 15-35 mM. However, high to moderate K+ concentrations increase Ca2+ influx and impair endothelial function. We have therefore evaluated the possible advantage of a lower potassium concentration in a new cardioplegic solution (named CRMBM solution) designed for long-term heart preservation. Nine isolated perfused rat hearts were submitted to 8 h of hypothermic ischemia after cardioplegic arrest, followed by 60 min of reflow at 37 degrees C. Two cardioplegic solutions were compared: (1) the CRMBM solution with 10 mM potassium (K-10 group), and (2) the CRMBM solution with 4 mM potassium (K-4 group). The quality of heart preservation was assessed by a metabolic study using P-31 magnetic resonance spectroscopy (energy metabolism and intracellular pH) combined to a functional evaluation and a measure of cellular integrity (biochemical assays in effluents and tissues). Decreasing the potassium concentration to 4 mM improved heart preservation, as shown by a higher functional post-ischemic recovery represented by the rate pressure product and a better preservation of cellular integrity. The evolutions of intracellular pH and high energy phosphate levels during ischemia and reflow were similar in both groups.
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine de Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Desrois
authors.forename: M
authors.initials: M
authors.lastname: Sciaky
authors.forename: C
authors.initials: C
authors.lastname: Lan
authors.forename: P J
authors.initials: PJ
authors.lastname: Cozzone
authors.forename: M
authors.initials: M
authors.lastname: Bernard
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002314:Cardioplegic Solutions / Q000737:chemistry*; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism; D006324:Heart Arrest, Induced; D006339:Heart Rate; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D011188:Potassium / Q000032:analysis*; D011687:Purines / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
chemical_list: D002314:Cardioplegic Solutions; D011687:Purines; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D011188:Potassium