title: Plasmodium chabaudi: effect of antimalarial drugs on gametocytogenesis.
abstract: The proportion of asexual blood-stage malaria parasites that develop into transmission stages (gametocytes) can increase in response to stress. We investigated whether stress imposed by a variety of antimalarial drugs administered before or during infection increased gametocyte production (gametocytogenesis) in vivo in the rodent malaria parasite, Plasmodium chabaudi. All methods of drug treatment greatly reduced the numbers of asexual parasites produced during an infection but resulted in either no reduction in numbers of gametocytes or a smaller reduction than that experienced by asexuals. We used a simple model to estimate temporal variation in gametocyte production. Temporal patterns of gametocytogenesis did not greatly differ between untreated and prophylaxis infections, with rates of gametocytogenesis always increasing as the infection progressed. In contrast, administration of drugs 5 days after infection stimulated increased rates of gametocytogenesis early in the infection, resulting in earlier peak gametocyte densities relative to untreated infections. Given the correlation between gametocyte densities and infectivity to mosquito vectors, and the high frequency of subcurative drug therapy and prophylaxis in human populations, these data suggest that antimalarial drugs may frequently have only a small effect on reducing malaria transmission and may help to explain the rapid spread of drug-resistant geno-types.
authors.affiliation: Institute of Cell, Animal & Population Biology, Division of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom. angus.buckling@plant-sciences.ox.ac.uk
authors.forename: A
authors.initials: A
authors.lastname: Buckling
authors.forename: L
authors.initials: L
authors.lastname: Crooks
authors.forename: A
authors.initials: A
authors.lastname: Read
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology* / Q000627:therapeutic use; D002738:Chloroquine / Q000494:pharmacology / Q000627:therapeutic use; D005718:Gametogenesis / Q000187:drug effects; D008288:Malaria / Q000188:drug therapy* / Q000469:parasitology / Q000517:prevention & control; D008297:Male; D015767:Mefloquine / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D016788:Plasmodium chabaudi / Q000187:drug effects* / Q000502:physiology; D011739:Pyrimethamine / Q000494:pharmacology / Q000627:therapeutic use; D011803:Quinine / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000962:Antimalarials; D002738:Chloroquine; D011803:Quinine; D015767:Mefloquine; D011739:Pyrimethamine