title: 2.0 A X-ray structure of the ternary complex of 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase from Escherichia coli with ATP and a substrate analogue.
abstract: The X-ray crystal structure of 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase (PPPK) in a ternary complex with ATP and a pterin analogue has been solved to 2.0 A resolution, giving, for the first time, detailed information of the PPPK/ATP intermolecular interactions and the accompanying conformational change. The first 100 residues of the 158 residue peptide contain a betaalpha betabeta alphabeta motif present in several other proteins including nucleoside diphosphate kinase. Comparative sequence examination of a wide range of prokaryotic and lower eukaryotic species confirms the conservation of the PPPK active site, indicating the value of this de novo folate biosynthesis pathway enzyme as a potential target for the development of novel broad-spectrum anti-infective agents.
authors.affiliation: Glaxo Wellcome R&D, Medicines Research Centre, Stevenage, UK.
authors.forename: D K
authors.initials: DK
authors.lastname: Stammers
authors.forename: A
authors.initials: A
authors.lastname: Achari
authors.forename: D O
authors.initials: DO
authors.lastname: Somers
authors.forename: P K
authors.initials: PK
authors.lastname: Bryant
authors.forename: J
authors.initials: J
authors.lastname: Rosemond
authors.forename: D L
authors.initials: DL
authors.lastname: Scott
authors.forename: J N
authors.initials: JN
authors.lastname: Champness
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004094:Dihydropteroate Synthase / Q000737:chemistry / Q000378:metabolism; D017854:Diphosphotransferases / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D011622:Pterins / Q000737:chemistry / Q000378:metabolism
chemical_list: D011622:Pterins; D000255:Adenosine Triphosphate; D004094:Dihydropteroate Synthase; D017854:Diphosphotransferases; C019684:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase