title: Interaction of Leishmania gp63 with cellular receptors for fibronectin.
abstract: The most abundant protein on the surface of the promastigote form of the protozoan parasites Leishmania spp. is a 63-kDa molecule, designated gp63 or leishmanolysin. Because gp63 has been shown to possess fibronectin-like properties, we examined the interaction of gp63 with the cellular receptors for fibronectin. We measured the direct binding of Leishmania to human macrophages or to transfected mammalian cells expressing human fibronectin receptors. Leishmania expressing gp63 exhibited modest but reproducible adhesion to human macrophages and to transfected CHO cells expressing alpha4/beta1 fibronectin receptors. In both cases, this interaction depended on gp63 but occurred independently of the SRYD sequence of gp63, because parasites expressing gp63 with a mutated SRYD sequence bound to macrophages and alpha4/beta1 receptor-expressing cells as well as did wild-type parasites. The contribution of gp63 to parasite adhesion was more pronounced when the assays were performed in the presence of complement, suggesting that the receptors for complement and fibronectin may cooperate to mediate the efficient adhesion of parasites to macrophages. The interaction of gp63 with fibronectin receptors may also play an important role in parasite internalization by macrophages. Erythrocytes to which gp63 was cross-linked were efficiently phagocytized by macrophages, whereas control erythrocytes opsonized with complement alone bound to macrophages but remained peripherally attached to the outside of the cell. Similarly, parasites expressing wild-type gp63 were rapidly and efficiently phagocytized by resting macrophages, whereas parasites lacking gp63 were internalized more slowly. This rapid internalization of gp63-expressing parasites was dependent on the beta1 integrins, because pretreatment of macrophages with monoclonal antibodies to the beta1 integrins decreased the internalization of gp63-expressing parasites. These observations indicate that complement receptors are the primary mediators of parasite adhesion; however, maximal parasite adhesion and internalization may require the participation of the beta1 integrins, which recognize fibronectin-like molecules such as gp63 on the surface of the parasite.
authors.affiliation: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
authors.forename: A
authors.initials: A
authors.lastname: Brittingham
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: B S
authors.initials: BS
authors.lastname: McGwire
authors.forename: K P
authors.initials: KP
authors.lastname: Chang
authors.forename: D M
authors.initials: DM
authors.lastname: Mosser
mesh_terms: D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D003176:Complement C3 / Q000378:metabolism; D006224:Cricetinae; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D007891:Leishmania / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D015800:Protozoan Proteins / Q000378:metabolism*; D017469:Receptors, Fibronectin / Q000235:genetics / Q000378:metabolism*; D016201:Receptors, Lymphocyte Homing / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D014162:Transfection
chemical_list: D003176:Complement C3; D039041:Integrin alpha4beta1; D016023:Integrins; D015800:Protozoan Proteins; D017469:Receptors, Fibronectin; D016201:Receptors, Lymphocyte Homing; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania