title: Modelling cortical cataractogenesis 21: in diabetic rat lenses taurine supplementation partially reduces damage resulting from osmotic compensation leading to osmolyte loss and antioxidant depletion.
abstract: The concentration of taurine and the amino acids, glutathione, cysteine, ascorbate and ATP were determined in the lenses of rats made diabetic with streptozotocin. In the clear lenses, prior to vacuole formation after 1 or 2 weeks of diabetes, the increase in concentration of sorbitol and the total decrease of all these osmolytes were not significantly different. The major components of the osmolytes lost were taurine and amino acids, which together accounted for over 75% of the total osmolyte loss. Since glutathione, ascorbate, taurine and cysteine have been reported to have antioxidant activity, it appears that their loss may potentiate damage occurring as a result of free radicals generated by nonenzymic glycation by the Maillard reaction. Amino acids also lost as a result of the osmotic compensation, are estimated to be responsible for almost half of the antioxidant activity lost. To test this hypothesis, normal and streptozotocin diabetic female Wistar rats were given taurine at 0.05% or 0.10% (w/w) in the diet. This treatment resulted in small only marginally significant increases in serum taurine levels. At the end of 6 weeks the rats were examined for weight gain or loss and at the time of killing, blood was collected for measurement of serum glucose. gamma-Crystallin levels were determined in vitreous and aqueous humours using a radioimmunoassay. A lens from each rat was homogenized in 8 m guanidinium chloride for adenosine triphosphate (ATP) analysis. In normal rats, a small amount of gamma-crystallin was found in the vitreous humour, and an even smaller amount in the aqueous humour. Diabetes caused a 4- to 5-fold increase in the vitreous humour and a 4-fold increase in gamma-crystallin in the aqueous humour. Diabetes also led to a significant worsening in general body condition, loss of body weight, formation of cataracts, and decrease in lens ATP levels. Addition of taurine to the diet of diabetic animals resulted in a significant decrease of gamma-crystallin leakage into the vitreous but not the aqueous humour. Taurine had no effect on the lens ATP levels. Neither streptozotocin diabetes nor taurine in the diet appeared to affect the weight of the lenses.
authors.affiliation: Department of Biochemistry, Faculty of Medicine and Dentistry, University of Western Ontario, London, Ontario, N6A 5C1, Canada.
authors.forename: K P
authors.initials: KP
authors.lastname: Mitton
authors.forename: H A
authors.initials: HA
authors.lastname: Linklater
authors.forename: T
authors.initials: T
authors.lastname: Dzialoszynski
authors.forename: S E
authors.initials: SE
authors.lastname: Sanford
authors.forename: K
authors.initials: K
authors.lastname: Starkey
authors.forename: J R
authors.initials: JR
authors.lastname: Trevithick
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002386:Cataract / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control*; D003459:Crystallins / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007908:Lens, Crystalline / Q000378:metabolism; D009995:Osmosis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000627:therapeutic use*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D001786:Blood Glucose; D003459:Crystallins; D013654:Taurine; D000255:Adenosine Triphosphate