Structure and promoter analysis of murine CAD and ICAD genes.
Caspase-activated DNase (CAD) degrades chromosomal DNA during apoptosis, whereas ICAD (inhibitor of CAD) inhibits the CAD's DNase by binding to it. Here, we describe the assignment of murine CAD and ICAD genes to the distal part of murine chromosome 4. Molecular cloning and structural analysis indicated that CAD and ICAD genes are comprised of 7 and 6 exons, respectively. Two different ICAD mRNAs coding for two forms of ICAD proteins (ICAD-S and ICAD-L) were found to be produced by alternative splicing of intron 5. The CAD and ICAD mRNAs were detected ubiquitously in various murine tissues. Analyses of the promoter activity with a series of deletion mutants of their 5' flanking regions indicated that a 190-bp 5' flanking region of the CAD gene was sufficient to promote the transcription. Whereas, a 120-bp flanking region of ICAD gene was required to promote its transcription. These regions do not show similarity between CAD and ICAD genes, suggesting that expression of CAD and ICAD genes is regulated by different mechanisms.
Kawane
K
K
Department of Genetics, Osaka University Medical School, Osaka, Japan.
Fukuyama
H
H
Adachi
M
M
Sakahira
H
H
Copeland
N G
NG
Gilbert
D J
DJ
Jenkin
N A
NA
Nagata
S
S
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors / Q000235:genetics*; D004791:Enzyme Inhibitors; D005260:Female; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D011506:Proteins; D012333:RNA, Messenger; C109664:caspase-activated DNase inhibitor; D003851:Deoxyribonucleases; C109663:caspase-activated deoxyribonuclease