title: Minichromosome maintenance as a genetic assay for defects in DNA replication.
abstract: Minichromosome maintenance (mcm) is an effective genetic assay for mutants defective in DNA replication. Two classes of mcm mutants have been identified using this screen: those that differentially affect the activities of certain autonomously replicating sequences (ARSs) and those that uniformly affect the activities of all ARSs. The ARS-specific MCM genes are essential for the initiation of DNA replication. Among these are members of the MCM2-7 family that encode subunits of the preinitiation complex and MCM10, whose gene product interacts with members of the Mcm2-7 proteins. Among the ARS-nonspecific MCM gene products are chromosome transmission factors. Refinement of this genetic assay as a screening tool and further analysis of existing mcm mutants may reveal new replication initiation proteins.
authors.affiliation: Section of Biochemistry Molecular and Cell Biology, Cornell University, Ithaca, New York 14853-2703, USA.
authors.forename: B K
authors.initials: BK
authors.lastname: Tye
mesh_terms: D002868:Chromosomal Proteins, Non-Histone; D015825:Chromosomes, Fungal; D004261:DNA Replication / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005816:Genetic Complementation Test; D005821:Genetic Techniques; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C069199:MCM2 protein, S cerevisiae