Genotoxic effects of triphenyltin acetate and triphenyltin hydroxide on mammalian cells in vitro and in vivo.
Two organotin pesticides, triphenyltin acetate (TPTA) and triphenyltin hydroxide (TPTH), were evaluated for their ability to induce micronuclei (MN) and sister chromatid exchange (SCE) in vitro using cultured Chinese hamster ovary (CHO) cells and in vivo BALB/c mouse erythrocytes. Both pesticides induced a dose-dependent increase but only TPTH induced a significant increase in MN at the highest dose (150 ng/ml) tested in CHO cells. With adding S9 microsomal fractions, both pesticides induced a meaningful MN induction at 150 ng/ml and a dose-dependent significant increase in SCE. In vivo MN induction in erythrocytes was conducted by treating BALB/c mice orally or intraperitoneally with these pesticides either in a single or triple treatments. Oral gavage (p.o.) of TPTA resulted in a dose-related significant increase of MN induction in peripheral blood and of TPTH induced a significant increase in micronucleated reticulocyte (MNRETs) only in a single treatment. Intraperitoneal administration of TPTA or TPTH, however, resulted in meaningless random increases in MN though these increases might be attributable to toxic effects. The MNRETs levels in the treatment with both pesticides were independent to the sampling time. This study demonstrated that TPTA and TPTH was potential chromosome mutagens.
Chao
J S
JS
National Laboratory Animal Breeding and Research Center, P.O. Box 1-86, Nankang 115, Taipei, Taiwan.
Wei
L Y
LY
Huang
M C
MC
Liang
S C
SC
Chen
H H
HH
D000818:Animals; D001711:Biotransformation; D016466:CHO Cells; D006224:Cricetinae; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005659:Fungicides, Industrial / Q000493:pharmacokinetics / Q000633:toxicity; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009947:Organotin Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects
D005659:Fungicides, Industrial; D009153:Mutagens; D009947:Organotin Compounds; C011978:phentin acetate; C007567:triphenyltin hydroxide