Umbilical cord blood mononuclear cell proliferative response to house dust mite does not predict the development of allergic rhinitis and asthma.
BACKGROUND
Cord blood mononuclear cells (CBMNCs) have been found to proliferate when stimulated with food or inhalant allergens. The significance of this finding is not known.


OBJECTIVE
We sought to determine the factors associated with proliferation of CBMNCs when stimulated with house dust mite (HDM) allergen and to determine whether proliferation of CBMNCs is associated with subsequent development of HDM sensitization and development of allergic rhinitis and asthma.


METHODS
Cord blood was taken from infants at high risk for having asthma and allergic diseases who were recruited for a primary prevention study in which infants were randomized into the intervention or the control group. CBMNCs were stimulated with HDM allergen and PHA as a positive control. Infants were assessed at 12 and 24 months for health outcome.


RESULTS
When stimulated with HDM allergen, 29.7% of infants had a stimulation index (SI) of 2 or greater. There were no differences in the levels of cord blood IgE and cotinine, maternal smoking, immediate skin test reactivity, maternal IgE, and maternal exposure to HDM allergen between those with an SI of 2 or greater and those with an SI of less than 2. Infants with an SI of less than 2 had a significantly higher proportion of mothers with a history of asthma. No differences in the incidence of "asthma" and rhinitis without cold at 12 months and cumulative prevalence at 24 months were seen between the 2 groups. None of the infants had skin test reactions to HDM allergen.


CONCLUSIONS
The negative association between HDM allergen-induced proliferation of CBMNCs and maternal mite allergen exposure and health outcomes at 12 and 24 months raises the issue of the significance of CBMNC proliferation.
Chan-Yeung
M
M
Occupational and Environmental Lung Disease Unit, Department of Medicine, University of British Columbia, Vancouver, Canada.
Ferguson
A
A
Chan
H
H
Dimich-Ward
H
H
Watson
W
W
Manfreda
J
J
Becker
A
A
D000485:Allergens / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000209:etiology*; D002455:Cell Division / Q000276:immunology; D004391:Dust / Q000009:adverse effects; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008925:Mites / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology*; D006255:Rhinitis, Allergic, Seasonal / Q000209:etiology*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust; D006023:Glycoproteins