Analysis of oestrogen receptor mRNA by reverse transcriptase-polymerase chain reaction in human periodontal ligament cells.
Periodontal ligament (PDL) cells have osteoblast-like features and are capable of differentiating into osteogenic cells. As human osteoblasts express oestrogen receptor mRNA, it is possible that PDL cells do so also, but findings have been conflicting. To determine whether they do express oestrogen receptor mRNA, the reverse transcriptase-polymerase chain reaction was performed with two different primers. Cells were obtained from a healthy periodontal ligament of premolar extracted for orthodontic reasons. The human breast adenocarcinoma cell-line MCF7 was used as a positive control. Expression of oestrogen receptor mRNA was detected in PDL cells with one of the primers but with less intensity than in MCF7 cells. Southern hybridization confirmed these results. These findings suggest that PDL cells express oestrogen receptor mRNA at low levels.
Morishita
M
M
Department of Preventive Dentistry, Hiroshima University School of Dentistry, Japan. mark@ipc.hiroshima-u.ac.jp
Shimazu
A
A
Iwamoto
Y
Y
D000230:Adenocarcinoma / Q000378:metabolism; D000328:Adult; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000378:metabolism; D002454:Cell Differentiation; D005260:Female; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D011960:Receptors, Estrogen / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D012194:RNA-Directed DNA Polymerase