title: Polyunsaturated fatty acids inhibit Mg2+ -ATPase in basolateral membranes from rat enterocytes.
abstract: It is known that certain polyunsaturated fatty acids of the n-6 family, for example linoleic and arachidonic acids, can activate both Na+, K+-ATPase and Ca2+-ATPase. These enzymes drive active absorption processes in the duodenal enterocyte. This study presents data which show a 30-50% inhibition of Mg2+-ATPase activity in enterocyte basolateral membrane preparations by linoleic and gamma-linolenic acids (also a member of the n-6 family.) Mg2+-ATPase activity has several possible roles in the enterocyte: involvement in Mg2+ and Ca2+ absorption (as part of Ca2+-ATPase and also myosin I activity) as well as control of phospholipid distribution in the membrane by a class of Mg2+-ATPases called 'flippases'. The action of linoleic and gamma-linolenic acids on basolateral membrane Mg2+-ATPase may thus modulate several cellular transport processes.
authors.affiliation: Department of Physiology, University of Pretoria, South Africa. mhaag@medic.up.ac.za
authors.forename: M
authors.initials: M
authors.lastname: Haag
authors.forename: F
authors.initials: F
authors.lastname: Vermeulen
authors.forename: O
authors.initials: O
authors.lastname: Magada
authors.forename: M C
authors.initials: MC
authors.lastname: Kruger
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors*; D002462:Cell Membrane / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D008041:Linoleic Acids / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017965:gamma-Linolenic Acid / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D017965:gamma-Linolenic Acid; D017301:Ca(2+) Mg(2+)-ATPase