Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy.
Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions.
Preza
C
C
Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110, USA. preza@ibc.wustl.edu
Snyder
D L
DL
Conchello
J A
JA
D005069:Evaluation Studies as Topic; D005544:Forecasting; D008027:Light; D008857:Microscopy, Interference / Q000592:standards; D008962:Models, Theoretical; D019047:Phantoms, Imaging; D010781:Photography / Q000295:instrumentation