[Detection of hematopoietic stem cells by flow cytometry, hematology analyzer or in vitro culture method].
We describe 3 different ways to determine the number of hematopoietic stem or progenitor cells in peripheral blood and harvest juice for peripheral blood stem cell transplantation. Flow cytometric analysis of CD34 positive cells gives us relatively quick and reliable results, although it's quite expensive. Automated hematology analyzer, SE-9000, exhibits real time appearance of stem cell population in a least expensive way. Colony formation in vitro such as CFU-GM is a direct way to detect hematopoietic committed cells, however this is time-consuming. We have to choose proper ways for each institute.
Saigo
K
K
Division of Blood Transfusion, Kobe University School of Medicine.
Hashimoto
M
M
Kumagai
S
S
Sugimoto
T
T
D001772:Blood Cell Count / Q000379:methods; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans