Identification of a third metalloprotease toxin gene in extraintestinal isolates of Bacteroides fragilis.
To further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.
Chung
G T
GT
Laboratory of Bacterial Toxins, Department of Microbiology, National Institute of Health, Seoul, Korea.
Franco
A A
AA
Wu
S
S
Rhie
G E
GE
Cheng
R
R
Oh
H B
HB
Sears
C L
CL
D000595:Amino Acid Sequence; D001442:Bacteroides Infections / Q000382:microbiology; D001441:Bacteroides fragilis / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007723:Korea; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data
D004269:DNA, Bacterial; D007527:Isoenzymes; D008666:Metalloendopeptidases; C091198:fragilysin