The hydroxyl free radical reactions of ascorbyl palmitate as measured in various in vitro models.
The OH(*) free radical scavenging properties of ascorbyl palmitate (AP), water-solubilized in the presence of a surfactant (Brij 35), were tested in various systems: (1) The inhibition of polymerization of bovine serum albumin by OH(*) free radicals generated by the Fenton reaction indicated AP exerts a considerable protective effect against polymerization by scavenging the OH(*) free radicals. (2) ESR spin trapping comparisons of DMPO with AP were conducted. Using the Fenton reaction as a source of OH(*) free radicals, AP was 1 order of magnitude faster in scavenging these radicals than DMPO. (3) Oxidative modification of BSA by (60)Co-gamma irradiation of 80 krad, results in a strong increase in protein carbonyl content. AP inhibits carbonyl formation very efficiently, indicating that AP may be utilized as a biological OH(*) free radical scavenger in human therapy.
Perricone
N
N
Department of Dermatology, Yale School of Medicine, New Haven, Connecticut, USA.
Nagy
K
K
Horváth
F
F
Dajkó
G
G
Uray
I
I
Zs-Nagy
I
I
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D002417:Cattle; D003037:Cobalt Radioisotopes; D004578:Electron Spin Resonance Spectroscopy; D005720:Gamma Rays; D006801:Humans; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000737:chemistry*; D007501:Iron; D007700:Kinetics; D000077423:Polidocanol; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects*; D013501:Surface-Active Agents
D003037:Cobalt Radioisotopes; C045076:Fenton's reagent; D013501:Surface-Active Agents; D000077423:Polidocanol; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D011092:Polyethylene Glycols; D006861:Hydrogen Peroxide; D007501:Iron; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid