title: 7-Amino-4-methyl-6-sulfocoumarin-3-acetic acid: a novel blue fluorescent dye for protein labeling.
abstract: 7-Amino-4-methyl-6-sulfocoumarin-3-acetic acid (AMCA-S, also called Alexa 350) 2 was synthesized as a new water-soluble blue fluorescent dye for protein labeling. Compared with its nonsulfonated counterpart (AMCA) 1 the new dye gave significantly higher fluorescence quantum yields on proteins.
authors.affiliation: Molecular Probes, Inc, Eugene, OR 97402, USA.
authors.forename: W Y
authors.initials: WY
authors.lastname: Leung
authors.forename: P A
authors.initials: PA
authors.lastname: Trobridge
authors.forename: R P
authors.initials: RP
authors.lastname: Haugland
authors.forename: R P
authors.initials: RP
authors.lastname: Haugland
authors.forename: F
authors.initials: F
authors.lastname: Mao
mesh_terms: D000085:Acetates / Q000138:chemical synthesis* / Q000737:chemistry; D002867:Chromones / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D000085:Acetates; C400304:Alexa 350; D002867:Chromones; D005456:Fluorescent Dyes; D011506:Proteins; D014867:Water