Inhibition of dextran sulphate sodium (DSS)-induced colitis in mice by intracolonically administered antibodies against adhesion molecules (endothelial leucocyte adhesion molecule-1 (ELAM-1) or intercellular adhesion molecule-1 (ICAM-1)).
We examined the effect of intracolonic administration of anti-adhesion molecule antibodies on DSS-induced colitis in mice. Immunohistochemical staining in mice with colitis showed increased expression of ELAM-1 and ICAM-1 on endothelial cells of vessels in the lamina propria and submucosa at sites of inflamed lesions. Intracolonic administration of anti-ELAM-1 or anti-ICAM-1 antibody decreased bloody stools, anaemia, and histologically evident damage, as well as myeloperoxidase activity and IL-1beta content. We concluded that adhesion molecule expression is important in the development of DSS-induced colitis in mice and that intracolonic administration of anti-adhesion molecule antibodies, especially anti-ELAM-1 antibody, effectively inhibits the colonic inflammation. Intracolonic administration of anti-adhesion molecule antibodies may show therapeutic promise in ulcerative colitis.
Hamamoto
N
N
Second Department of Internal Medicine, Osaka Medical College, Osaka, Japan. makimaki@d1.dion.ne.jp
Maemura
K
K
Hirata
I
I
Murano
M
M
Sasaki
S
S
Katsu
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D016264:Dextran Sulfate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000096:biosynthesis / Q000276:immunology*; D005260:Female; D007167:Immunotherapy; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000276:immunology*; D007375:Interleukin-1 / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D007375:Interleukin-1; D018799:Intercellular Adhesion Molecule-1; D016264:Dextran Sulfate; D009195:Peroxidase