In vitro anti-human immunodeficiency virus activity of polysaccharide from Rhizophora mucronata Poir.
A polysaccharide was extracted with 1% sodium carbonate from the bark of Rhizophora mucronata and its antiviral activities against human immunodeficiency virus (HIV) were assessed by an in vitro cell culture system. The anti-HIV activity of the alkaline extract was mainly recovered in the 25-75% ethanol-precipitated fraction. Rhizophora mucronata polysaccharide (RMP) protected MT-4 cells from the HIV-induced cytopathogenicity and blocked the expression of HIV antigens. RMP completely inhibited the viral binding to the cell and the formation of syncytium upon cocultivation of MOLT-4/HIV-1IIIB cells and MOLT-4 cells. These results suggest that RMP inhibited early steps of the virus life cycle especially virus adsorption to the cell.
Premanathan
M
M
Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
Kathiresan
K
K
Yamamoto
N
N
Nakashima
H
H
D019380:Anti-HIV Agents / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D015726:Giant Cells / Q000737:chemistry; D015488:HIV Antigens / Q000096:biosynthesis; D015497:HIV-1 / Q000187:drug effects / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007194:India; D007202:Indicators and Reagents; D019441:Plant Epidermis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D012997:Solvents
D019380:Anti-HIV Agents; D015488:HIV Antigens; D007202:Indicators and Reagents; D011134:Polysaccharides; D012997:Solvents