GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode.
This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell growth rates are important. Using a standard fluorometer, growth of these cells can be quantified noninvasively in multiwell plates, and because signals are obtained without preparation, the same culture samples can be measured repeatedly. Even with a small relative change in biomass, the specific growth rate can be determined in a few hours. The dynamics of cell populations can now be studied with high sensitivity, low error rate, and minimum sample preparation.
Hunt
L
L
Laboratoire de Biotechnologie Cellulaire, Institut de Génie Chimique, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.
Jordan
M
M
De Jesus
M
M
Wurm
F M
FM
D000818:Animals; D016466:CHO Cells; D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival; D006224:Cricetinae; D003470:Culture Media; D005470:Fluorometry; D049452:Green Fluorescent Proteins; D007700:Kinetics; D008164:Luminescent Proteins / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000032:analysis
D003470:Culture Media; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins