Modified technique for kidney transplantation in mice.
This study describes a new method for joining the donor ureter to the recipient bladder during mouse kidney transplantation. The donor left kidney was harvested using methods previously published, except that bladder tissue was not harvested with the end of the ureter. The recipient left kidney was removed and the donor kidney was attached using end-to-side anastomosis. The recipient bladder was pierced with a 21-gauge needle allowing curved forceps to be inserted through the bladder, to pull through the ureter, and the periuretal tissue was stitched to the exterior wall of the bladder. The donor ureter was allowed to retract inside the bladder. Following a right nephrectomy, grafts were monitored by blood serum creatinine and urea. With a technical success rate of 83%, this technique reduced donor harvest time by 20 minutes and ureter attachment time by 15 minutes making it the best method available for mouse kidney transplantation.
Han
W R
WR
Department of Surgery, University of Melbourne, Melbourne, Victoria, Australia.
Murray-Segal
L J
LJ
Mottram
P L
PL
D000818:Animals; D005260:Female; D016030:Kidney Transplantation / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013536:Suture Techniques; D013997:Time Factors; D014513:Ureter / Q000601:surgery; D001743:Urinary Bladder / Q000601:surgery