Morphological observation of canine natural killer cells mediated cytotoxicity.
The cytotoxic effects of canine NK cells on CL-1 target cells were examined by scanning electron microscopy (SEM). NK cell mediated cytotoxicity on CL-1 target cells was detected by 51Cr release assay. SEM showed that a canine NK cell extended projections to the CL-1 target cell. Furthermore, the surface of CL-1 target cells changed a mesh-like structure. Therefore, the cytotoxic effects of canine NK cells on CL-1 target cells were morphologically demonstrated.
Nariai Nakada
Y
Y
Laboratory Animal Facility, School of Medicine, Yokohama City University, Yokohama, Kanagawa, Japan.
Nariai
K
K
Kosaka
T
T
Kuwabara
M
M
Kiuchi
Y
Y
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology; D002469:Cell Separation; D002501:Centrifugation, Zonal; D003602:Cytotoxicity, Immunologic; D004285:Dogs; D005260:Female; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology