Removal of detergent and solvent from solvent-detergent-treated immunoglobulins.
The solvent-detergent (S/D) method was applied for inactivation of lipid-enveloped viruses during the production of immunoglobulins. Amberlite XAD-7 resin was used for removal of solvent (tri-n-butyl phosphate, TnBP) and detergent (Triton X-100) after the performed S/D inactivation procedure. The S/D reagents from the immunoglobulin preparation were adsorbed on Amberlite XAD-7, while immunoglobulins passed through the column and retained their biological activity. Using the method developed here, the final immunoglobulin preparation contains less than 1 ppm of Triton X-100 and less than 2 ppm TnBP.
Trescec
A
A
Institute of Immunology, University of Zagreb, Croatia.
Simić
M
M
Branović
K
K
Gebauer
B
B
Benko
B
B
D000180:Acrylic Resins; D000837:Anion Exchange Resins; D003902:Detergents / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000302:isolation & purification*; D011137:Polystyrenes; D012997:Solvents / Q000737:chemistry / Q000302:isolation & purification*
D000180:Acrylic Resins; D000837:Anion Exchange Resins; D003902:Detergents; D007136:Immunoglobulins; D011137:Polystyrenes; D012997:Solvents; C015661:amberlite XAD 7