[Estimation of bleomycin-induced chromosome aberrations in lymphocytes of laryngeal cancer subjects. Preliminary report].
Chromosome instability is associated with an increased risk of malignancy. However, the quantitative analysis of chromosome breaks provided by the bleomycin test requires additional analysis aimed for the localisation of chromosome aberrations. For this reason, the metaphasis slides prepared for bleomycin test were stained with fluorochrome DAPI to estimate chromosome breaks in particular chromosomes. The additional staining of chromosomes can be recognised as an extension of the classical bleomycin test addressed for identification of structural aberrations. Preliminary results indicate that the most frequent chromosome breaks were found in chromosomes 1, 2, 3, 7 and 13.
Kita
S
S
Studenckie Koło Naukowe przy Klinice Otolaryngologii AM im. K. Marcinkowskiego w Poznaniu.
Jarmuz
M
M
Dabrowski
P
P
Biegalski
W
W
Jezewska
A
A
Kowalczyk
M
M
Szyfter
W
W
Szyfter
K
K
D000368:Aged; D000369:Aged, 80 and over; D001761:Bleomycin; D002869:Chromosome Aberrations / Q000175:diagnosis* / Q000235:genetics; D025063:Chromosome Disorders; D002900:Chromosomes, Human, 1-3; D002882:Chromosomes, Human, Pair 13; D002897:Chromosomes, Human, Pair 7; D005819:Genetic Markers; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged
D005819:Genetic Markers; D001761:Bleomycin