Semi-automated rapid isoelectric focusing of apolipoproteins C from human plasma using Phastsystem and immunofixation.
Apolipoproteins (apo) C-I, C-II, and C-III play crucial roles in intravascular lipid metabolism. Whereas apo C-II is an obligate cofactor for lipoprotein lipase, apo C-III was shown to inhibit its action. Apo C-I can be a potent cofactor of human lecithin:cholesterol acyltransferase. Structural mutants and deficiencies of apo C-II lead to hypertriglyceridemia. A similar phenotype is associated with apo C-III mutants and is inducible by overexpression of human apo C-III in transgenic animals. No structural variant has so far been reported for apo C-I. The present paper describes a rapid semi-automated procedure for isoelectric focusing analysis of these C-apolipoproteins from whole plasma or serum and their visualization by immunofixation and silver staining. The procedure allows detection of charged variants of C-apolipoproteins. As applied to 295 patients with coronary heart disease and 85 controls, it also serves to detect deficiency syndromes of these apolipoproteins. The procedure provides reliable, easy and quick analysis of C-apolipoproteins applicable as a routine or screening procedure not restricted to specialized laboratories.
Noll
B
B
Zentrum Innere Medizin, Abteilung Kardiologie, Klinikum der Philipps Universität Marburg, Germany.
Hackler
R
R
Pelzer
M
M
Pelzer
S
S
Nusser
P
P
Maisch
B
B
Schaefer
J R
JR
Steinmetz
A
A
D000180:Acrylic Resins; D001056:Apolipoproteins C / Q000097:blood* / Q000302:isolation & purification; D001322:Autoanalysis; D001797:Blood Protein Electrophoresis / Q000379:methods; D003327:Coronary Disease / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods; D011233:Precipitin Tests; D015203:Reproducibility of Results
D000180:Acrylic Resins; D001056:Apolipoproteins C; C016680:polyacrylamide gels