Tandem arrayed ligation of expressed sequence tags (TALEST): a new method for generating global gene expression profiles.
We have developed a new and simple method for quantitatively analyzing global gene expression profiles from cells or tissues. The process, called TALEST, or tandem arrayed ligation of expressed sequence tags, employs an oligonucleotide adapter containing a type IIs restriction enzyme site to facilitate the generation of short (16 bp) ESTs of fixed position in the mRNA. These ESTs are flanked by GC-clamped punctuation sequences which render them resistant to thermal denaturation, allowing their concatenation into long arrays and subsequent recognition and analysis by high-throughput DNA sequencing. A major advantage of the TALEST technique is the avoidance of PCR in all stages of the process and hence the attendant sequence-specific amplification biases that are inherent in other gene expression profiling methods such as SAGE, Differential Display, AFLP, etc. which rely on PCR.
Spinella
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Chugai Biopharmaceuticals, Inc., 6275 Nancy Ridge Drive, San Diego, CA 92121, USA. dspinella@chugaibio.com
Bernardino
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Redding
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Koutz
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Wei
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Pratt
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Myers
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Chappell
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Gerken
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McConnell
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D000328:Adult; D018076:DNA, Complementary; D004926:Escherichia coli; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015894:Genome, Human; D006801:Humans; D008168:Lung / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012984:Software
D018076:DNA, Complementary; D012333:RNA, Messenger