Accumulation of mercury in neurosecretory neurons of mice after long-term exposure to oral mercuric chloride.
Inorganic mercury (HgCl2) was administered to adult mice in drinking water (20 mg/l). Animals were sacrificed after one or two years and fixed by whole-body perfusion. Sections of the hypothalamus and neurohypophysis were subjected to silver acetate autometallography for visualization of mercury at light and electron microscopy levels. Mercury deposits, which can be seen by light microscopy as black granules, were found to accumulate within neuronal perykaria of the supraoptic and paraventricular nuclei. Electron microscopy demonstrated that mercury deposits in neurosecretory neurons were detected exclusively within lysosomes. Mercury was also present in small vesicles, 40-70 nm in diameter, and in endocytic vacuoles within the axon terminals of the neurohypophysis. No mercury could be seen in sections obtained from control animals that had been drinking uncontaminated water. Mechanisms involved in uptake and transport of mercury to neuronal bodies are discussed.
Villegas
J
J
Department of Anatomy and Cell Biology, School of Medicine, University of Cantabria, Santander, Spain. villegaj@medi.unican.es
Martinez
R
R
Andres
A
A
Crespo
D
D
D000284:Administration, Oral; D000818:Animals; D005260:Female; D008297:Male; D008627:Mercuric Chloride / Q000008:administration & dosage* / Q000378:metabolism; D008628:Mercury / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009490:Neurosecretory Systems / Q000378:metabolism* / Q000648:ultrastructure; D013495:Supraoptic Nucleus / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D008627:Mercuric Chloride; D008628:Mercury