title: An archaeal aminoacyl-tRNA synthetase missing from genomic analysis.
abstract: The complete genomic sequencing of Methanococcus jannaschii cannot identify the gene for the cysteine-specific member of aminoacyl-tRNA synthetases. However, we show here that enzyme activity is present in the cell lysate of M. jannaschii. The demonstration of this activity suggests a direct pathway for the synthesis of cysteinyl-tRNA(Cys) during protein synthesis.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: C S
authors.initials: CS
authors.lastname: Hamann
authors.forename: K R
authors.initials: KR
authors.lastname: Sowers
authors.forename: R S
authors.initials: RS
authors.lastname: Lipman
authors.forename: Y M
authors.initials: YM
authors.lastname: Hou
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D019766:Genes, Archaeal; D016678:Genome; D007700:Kinetics; D017017:Methanococcus / Q000201:enzymology* / Q000235:genetics*; D013816:Thermodynamics
chemical_list: D000604:Amino Acyl-tRNA Synthetases; C019975:cysteinyl-tRNA synthetase