title: Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera: Geotrupidae) using banding and fluorescent in-situ hybridization.
abstract: Mitotic metaphase chromosomes of the scarab beetle Thorectes intermedius (Costa) (Coleoptera Scarabaeoidea: Geotrupidae) were analyzed using various banding methods and fluorescent in-situ hybridization (FISH) with a ribosomal probe. The results obtained indicate that silver and CMA3 staining are unable to localize the chromosome sites of nucleolar organizer regions (NORs). Such an inadequacy is a consequence of the extensive silver and CMA3 stainability of both constitutive heterochromatin and heterochromatin associated to the NORs.
authors.affiliation: Dipartimento di Biologia Animale, Università di Palermo, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Vitturi
authors.forename: M S
authors.initials: MS
authors.lastname: Colomba
authors.forename: R
authors.initials: R
authors.lastname: Barbieri
authors.forename: M
authors.initials: M
authors.lastname: Zunino
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D001517:Coleoptera / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region
chemical_list: D004275:DNA, Ribosomal