title: Analysis of rate of change of IS6110 RFLP patterns of Mycobacterium tuberculosis based on serial patient isolates.
abstract: The rate of change of IS6110 restriction fragment length polymorphism (RFLP) patterns of Mycobacterium tuberculosis was determined in serial isolates from 544 patients. In 25 patients (4.6%), the RFLP patterns of the follow-up isolates differed from the initial isolates. Patients with different follow-up strains were less likely to cluster with patients whose strains had indistinguishable RFLP patterns. Changes in RFLP patterns were more common for persons with extrapulmonary disease and for those who had both pulmonary and extrapulmonary isolates. Based on serial isolates spanning for the most part <3 months, the half-life was extrapolated to be 3.2 years (95% confidence interval, 2.1-5.0). The main implication of this study is that the rate of change of IS6110-based RFLP of M. tuberculosis supports the use of IS6110 typing in epidemiologic studies of recent transmission of tuberculosis.
authors.affiliation: National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands. Annette.de.Boer@rivm.nl
authors.forename: A S
authors.initials: AS
authors.lastname: de Boer
authors.forename: M W
authors.initials: MW
authors.lastname: Borgdorff
authors.forename: P E
authors.initials: PE
authors.lastname: de Haas
authors.forename: N J
authors.initials: NJ
authors.lastname: Nagelkerke
authors.forename: J D
authors.initials: JD
authors.lastname: van Embden
authors.forename: D
authors.initials: D
authors.lastname: van Soolingen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004251:DNA Transposable Elements; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D009316:National Institutes of Health (U.S.); D009426:Netherlands; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*; D014481:United States
chemical_list: D004251:DNA Transposable Elements