Chemokine gene expression in human oral mucosa.
In order to gain further understanding of the role of chemokines in healthy oral mucosa, we analyzed mRNA expression of the alpha (CXC)-family chemokines IL-8 and GROgamma as well as of the beta (CC)-family chemokines MIP-1alpha, MIP-1beta and MCP-1 in twenty young and healthy subjects with good oral hygiene. Twenty biopsies were taken from clinically healthy oral mucosa before surgical removal of impacted wisdom teeth. In addition, five biopsies from patients presenting with specific oral lesions were studied. RNA was purified, quantitated and utilized as substrate for competitive reverse transcription-polymerase chain reaction (RT-PCR). In healthy tissue, IL-8 and MCP-1 mRNA was constitutively expressed in all biopsies, whereas GROgamma, MIP-1alpha, and MIP-1beta were significantly lower. These findings suggest that IL8 and MCP-1 play a significant role in oral tissue homeostasis. The few samples from pathological conditions encourage exploring diseased tissue in more detail.
Zehnder
M
M
Department of Removable Prosthodontics, School of Dental Medicine, University of Bern, Switzerland.
Greenspan
J S
JS
Greenspan
D
D
Bickel
M
M
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001706:Biopsy; D018932:Chemokine CCL2 / Q000235:genetics; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054360:Chemokine CXCL1; D019742:Chemokines, CC / Q000235:genetics*; D019743:Chemokines, CXC / Q000235:genetics*; D002630:Chemotactic Factors / Q000235:genetics; D005260:Female; D005350:Fibroma / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D005887:Gingival Neoplasms / Q000235:genetics / Q000276:immunology; D006131:Growth Inhibitors / Q000235:genetics; D006133:Growth Substances / Q000235:genetics; D006487:Hemostasis / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000235:genetics; D017676:Lichen Planus, Oral / Q000235:genetics / Q000276:immunology; D016483:Lymphoma, AIDS-Related / Q000235:genetics / Q000276:immunology; D019402:Macrophage Inflammatory Proteins / Q000235:genetics; D008297:Male; D009059:Mouth Diseases / Q000235:genetics / Q000276:immunology*; D009061:Mouth Mucosa / Q000276:immunology*; D009062:Mouth Neoplasms / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
C475829:CXCL1 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054360:Chemokine CXCL1; D019742:Chemokines, CC; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger