title: The effect of age and natural priming on the IgG and IgA subclass responses after parenteral influenza vaccination.
abstract: This study investigated the effect of natural priming and age on serum IgG and IgA subclass responses after parenteral trivalent influenza vaccination. Sera from 18 young children and 8 adults were collected at various times after vaccination. An ELISA was performed to quantify the concentrations of antibody subclasses. The children were divided into primed and unprimed groups based on the presence of prevaccination serum antibodies. In both children and adults, IgG1 and IgA1 were the predominant IgG and IgA subclasses detected after vaccination. No IgG2 responses were detected in sera of unprimed children, and the proportion of the IgG2 response was lower in primed children than in adults. This suggests that the IgG2 immune response in young children is dependent on previous priming and may mature later than the other IgG subclasses after parenteral influenza vaccination.
authors.affiliation: The Norwegian Medicines Control Authority, Sven Oftedals vei 6, N-0950 Oslo, Norway. Abdullah.el-madhun@slk.no
authors.forename: A S
authors.initials: AS
authors.lastname: El-Madhun
authors.forename: R J
authors.initials: RJ
authors.lastname: Cox
authors.forename: L R
authors.initials: LR
authors.lastname: Haaheim
mesh_terms: D000328:Adult; D000367:Age Factors; D000914:Antibodies, Viral / Q000097:blood* / Q000145:classification; D000917:Antibody Formation; D002675:Child, Preschool; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood* / Q000145:classification; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology*; D007273:Injections, Intramuscular; D008875:Middle Aged; D014068:Tonsillectomy
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines