title: Changes in endogenous polyamine levels are associated with differentiation in Dictyostelium discoideum.
abstract: This is the first report correlating levels of polyamines and its fractions with differentiation in Dictyostelium discoideum. Temporal changes in endogenous levels of free, conjugated and bound putrescine, spermidine and spermine were analysed at critical stages of morphogenesis in this organism. No spermine was found at any given stage and putrescine was the most abundant polyamine. There was a sharp increase in the levels of both free (and total) and conjugated forms of putrescine and spermidine at the slug stage as compared to the growth phase. The levels of putrescine and spermidine were found to be higher in isolated prespore cells as compared to the prestalk cells. Remarkably, the levels of polyamine decreased at the early culminant stage. Data suggest that a moderate level of polyamines is needed for growth but it is important to have high levels of polyamines at the time of differentiation.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi-, 110 067, India.
authors.forename: S
authors.initials: S
authors.lastname: Saran
mesh_terms: D000818:Animals; D004023:Dictyostelium / Q000254:growth & development / Q000378:metabolism*; D008018:Life Cycle Stages; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
chemical_list: D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine