Physical mapping of the G-protein coupled receptor 19 (GPR19) in the chromosome 12p12.3 region frequently rearranged in cancer cells.
Rearrangements of the short arm of chromosome 12 are frequently observed in hematological malignancies and in certain solid neoplasias. Loss of heterozygosity studies identified a small genetic interval on chromosome 12p12.3 that is frequently deleted in childhood acute lymphoblastic leukemia (ALL). Two genes, ETV6 and CDKN1B, are located within this interval although evidence has accumulated that an as yet unidentified tumor suppressor gene is closely linked. Here we report the physical mapping of the G-protein coupled receptor 19 (GPR19) at approximately 40kb from CDKN1B. The delineation of the gene order tel-ETV6-CDKN1B-GPR19-cen excluded GPR19 from the region commonly deleted in childhood ALL, but it could still be the target of genetic alterations found in other cancers.
Montpetit
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A
Division of Hematology-Oncology, Charles-Bruneau Cancer Center, Sainte-Justine Hospital, Montreal, Quebec, Canada.
Sinnett
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D015139:Blotting, Southern; D018797:Cell Cycle Proteins; D002881:Chromosomes, Human, Pair 12; D018899:CpG Islands; D050760:Cyclin-Dependent Kinase Inhibitor p27; D019204:GTP-Binding Proteins / Q000235:genetics*; D015723:Gene Library; D015321:Gene Rearrangement; D008040:Genetic Linkage; D006801:Humans; D018895:Microsatellite Repeats; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008957:Models, Genetic; D009369:Neoplasms / Q000235:genetics*; D009419:Nerve Tissue Proteins; D020161:Physical Chromosome Mapping; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; C509756:GPR19 protein, human; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D019204:GTP-Binding Proteins