title: Synthesis of new photocross-linking 5-C-base-substituted UTP analogs and their application in highly selective affinity labelling of the tick-borne encephalitis virus RNA replicase proteins.
abstract: A new photocross-linking 5-C-base-substituted UTP analogs, carrying 4-azidoperfluorobenzoyl and 4-azidoaniline residues were synthesized. Two flavivirus proteins NS5 and NS3 are shown to be labelled after RNA synthesis in the presence of the analogs, irradiation (lambda > 300 nm) and subsequent [alpha-32P]NTP incorporation.
authors.affiliation: Institute of Bioorganic Chemistry, Novosibirsk, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Morozova
authors.forename: D M
authors.initials: DM
authors.lastname: Kolpashchikov
authors.forename: T M
authors.initials: TM
authors.lastname: Ivanova
authors.forename: T S
authors.initials: TS
authors.lastname: Godovikova
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D004669:Encephalitis Viruses, Tick-Borne / Q000201:enzymology*; D010777:Photochemistry; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D013057:Spectrum Analysis; D013552:Swine; D014544:Uridine Triphosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000345:Affinity Labels; D012324:RNA-Dependent RNA Polymerase; D014544:Uridine Triphosphate