title: Gene targeting restricted to mouse striated muscle lineage.
abstract: Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM, CNRS, ULP, BP163, 67404 Illkirch cedex, C.U. de Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Miniou
authors.forename: D
authors.initials: D
authors.lastname: Tiziano
authors.forename: T
authors.initials: T
authors.lastname: Frugier
authors.forename: N
authors.initials: N
authors.lastname: Roblot
authors.forename: M
authors.initials: M
authors.lastname: Le Meur
authors.forename: J
authors.initials: J
authors.lastname: Melki
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002645:Chickens; D018390:Gene Targeting; D006801:Humans; D019426:Integrases / Q000235:genetics*; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014764:Viral Proteins
chemical_list: D000199:Actins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases