title: Population structure of the primary malaria vector in South America, Anopheles darlingi, using isozyme, random amplified polymorphic DNA, internal transcribed spacer 2, and morphologic markers.
abstract: A genetic and morphologic survey of Anopheles darlingi populations collected from seven countries in Central and South America was performed to clarify the taxonomic status of this major malaria vector species in the Americas. Population genetics was based on three techniques including isozyme, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), and internal transcribed spacer 2 (ITS2) markers. The results of the isozyme analysis indicated moderate differences in the allele frequencies of three putative loci (glutamate oxalaoacetate transaminase-1, isocitrate dehydrogenase-1, and phosphoglucomutase) of the 31 analyzed. No fixed electromorphic differences separated the populations of An. darlingi, which showed little genetic divergence (Nei distances = 0.976-0.995). Fragments produced by RAPD-PCR demonstrated evidence of geographic partitioning and showed that all populations were separated by small genetic distances as measured with the 1 - S distance matrix. The ITS2 sequences for all samples were identical except for four individuals from Belize that differed by a three-base deletion (CCC). The morphologic study demonstrated that the Euclidean distances ranged from 0.02 to 0.14, with the highest value observed between populations from Belize and Bolivia. Based on these analyses, all the An. darlingi populations examined demonstrated a genetic similarity that is consistent with the existence of a single species and suggest that gene flow is occurring throughout the species' geographic range.
authors.affiliation: Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
authors.forename: S
authors.initials: S
authors.lastname: Manguin
authors.forename: R C
authors.initials: RC
authors.lastname: Wilkerson
authors.forename: J E
authors.initials: JE
authors.lastname: Conn
authors.forename: Y
authors.initials: Y
authors.lastname: Rubio-Palis
authors.forename: J A
authors.initials: JA
authors.lastname: Danoff-Burg
authors.forename: D R
authors.initials: DR
authors.lastname: Roberts
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D001531:Belize; D004592:Electrophoresis, Starch Gel / Q000662:veterinary; D005260:Female; D007303:Insect Vectors / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D007521:Isocitrate Dehydrogenase / Q000737:chemistry; D007527:Isoenzymes / Q000032:analysis; D008288:Malaria / Q000635:transmission*; D008969:Molecular Sequence Data; D010733:Phosphoglucomutase / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013020:South America
chemical_list: D007527:Isoenzymes; D007521:Isocitrate Dehydrogenase; D010733:Phosphoglucomutase