The 9-fluorenylmethoxycarbonyl (Fmoc) group and its use in oligonucleotide synthesis.
The introduction of the base-labile 9-fluorenylmethoxycarbonyl (Fmoc) group into the exocyclic amino function of 2'-deoxynucleosides and their dimethoxytritylation and phosphitylation is described. The resulting key intermediates were investigated in the built-up of different oligodeoxyribonucleoside phosphate and thiophosphate chains which were deprotected under mild basic conditions leading to crude oligomers of high purity.
Schirmeister-Tichy
H
H
Fakultät für Chemie, Universität Konstanz.
Alvarado
G G
GG
Pfleiderer
W
W
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005449:Fluorenes / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis*
D005449:Fluorenes; D009841:Oligonucleotides