title: Contractile effect of (+)-glaucine in the isolated guinea-pig ileum.
abstract: The intestinal effects of (+)-glaucine [(S)-1,2,9,10-tetramethoxyaporphine] were studied using the guinea-pig ileum. (+)-Glaucine (10-300 microM) induced ileal contractions. The contraction was not affected by tetrodotoxin, atropine, hexamethonium, propranolol, naloxone, methysergide, N(G)-nitro-L-arginine methyl ester, SR141716A (a cannabinoid CB1 receptor antagonist) or SR140333 (a tackykinin NK1 receptor antagonist) plus SR48968 (a tackykinin NK2 antagonist). (+)-Glaucine-induced contraction was reduced by indomethacin, nordihydroguaiaretic acid or bisindolylmaleimide I and abolished by verapamil and nifedipine. These results suggest that (+)-glaucine-induced contraction involves activation of voltage-dependent Ca2+ channels and protein kinase C and could be mediated by the release of arachidonic acid metabolites.
authors.affiliation: Department of Experimental Pharmacology, University of Naples Federico II, Italy.
authors.forename: A A
authors.initials: AA
authors.lastname: Izzo
authors.forename: F
authors.initials: F
authors.lastname: Borrelli
authors.forename: F
authors.initials: F
authors.lastname: Capasso
authors.forename: R
authors.initials: R
authors.lastname: Capasso
authors.forename: L
authors.initials: L
authors.lastname: Pinto
authors.forename: A
authors.initials: A
authors.lastname: Cristoni
authors.forename: N
authors.initials: N
authors.lastname: Mascolo
mesh_terms: D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D001060:Aporphines; D015220:Calcium Channels; D010276:Parasympatholytics; D011493:Protein Kinase C; C005004:glaucine