Inducible isopentenyl transferase as a high-efficiency marker for plant transformation.
Overexpression of the isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an antibiotic-resistance marker. The combined system allows efficient cointroduction of multiple genes (in addition to ipt) and produces transgenic plants without morphological or developmental defects.
Kunkel
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Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Ave., New York, NY 10021-6399, USA.
Niu
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Chan
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Chua
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D016960:Agrobacterium tumefaciens / Q000235:genetics; D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis / Q000235:genetics*; D003583:Cytokinins / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction; D005798:Genes, Bacterial; D005819:Genetic Markers; D018545:Lactuca / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D019684:Magnoliopsida / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D012641:Selection, Genetic; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic
D003583:Cytokinins; D005819:Genetic Markers; D003907:Dexamethasone; D008156:Luciferases; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase