Crystallization and preliminary diffraction data of 60-kDa glycosylated pollen isoallergens from Bermuda grass.
Crystals grown from a 60-kDa isoallergen mixture of Bermuda grass pollen have been obtained in 30% PEG 4000 and 25% isopropanol. The crystals diffract beyond 2-A resolution and belong to a tetragonal space group with the unit cell dimensions a = b = 86 A and c = 310 A. The preferential crystal growth of the larger isoallergens with a blocked N-terminus indicates that crystallization can isolate proteins with compact conformation.
Liaw
S H
SH
Department of Life Science, National Yang-Ming University, Taipei, Taiwan. shliaw@ym.edu.tw
Lee
D Y
DY
Yang
S Y
SY
Su
S N
SN
D000485:Allergens / Q000648:ultrastructure*; D003460:Crystallization; D018360:Crystallography, X-Ray; D003851:Deoxyribonucleases; D005982:Glutathione Transferase; D006031:Glycosylation; D007519:Isoantigens / Q000276:immunology; D006109:Poaceae / Q000737:chemistry* / Q000648:ultrastructure; D011058:Pollen / Q000737:chemistry* / Q000648:ultrastructure
D000485:Allergens; D007519:Isoantigens; D005982:Glutathione Transferase; D003851:Deoxyribonucleases