Mast cells contribute to PACAP-induced dermal oedema in mice.
In the present study the effect of intradermal PACAP-injection on dermal oedema in mice was investigated and the contribution of mast cells to this response was assessed. The injection of PACAP 1-38 into the ears of C57BL/6 mice evoked a dose-dependent response, which, after higher doses of PACAP 1-38, lasted at least 24 h. Histological examination showed significant mast cell degranulation induced by PACAP. Using mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice and the congenic mice, we demonstrated that the the early phase (30 min to 6 h) of PACAP-induced ear swelling response was significantly diminished in mast cell-deficient mice, suggesting that mast cell degranulation contributes to this phase of the response. When mast cell-deficient WBB6F1-Kit(W)/Kit(W-v) mice were locally and selectively reconstituted by adoptive mast cell transfer, the dermal oedema was almost equal to that of control animals in the early phase of PACAP injection. These results show that mast cell degranulation contributes to PACAP-induced dermal oedema in mice.
Schmidt-Choudhury
A
A
Department of Pediatrics, University of Kiel, Germany.
Furuta
G T
GT
Galli
S J
SJ
Schmidt
W E
WE
Wershil
B K
BK
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects; D004423:Ear; D004487:Edema / Q000139:chemically induced / Q000209:etiology* / Q000503:physiopathology; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009479:Neuropeptides / Q000008:administration & dosage / Q000009:adverse effects*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012871:Skin Diseases; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000009:adverse effects
C495402:Adcyap1 protein, mouse; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D014665:Vasodilator Agents