Epidermal tissue-derived T-cell growth factor, colony stimulating factor and nerve growth factor in chickens.
The purpose of this study was to determine the mechanism of the local cytokine-mediated immune response in the skin of chickens. The incorporation of 3H-thymidine into spleen T lymphocytes from 9-to 10-week-old chickens was augmented by the addition of epidermal tissue culture supernatant (ESN) from 11-day-old embryos. The colony formation of neonatal chicken bone marrow cells in the methylcellulose medium was also significantly increased by addition of ESN. When axonal outgrowth in matrigel was investigated, the embryonal sympathetic ganglion was found to grow axons outwards towards the epidermal tissue specimens. The above results suggest that chicken epidermal cells (probably keratinocytes) produce T-cell growth factor (corresponding to IL-1), colony-stimulating factor for macrophages (M-CSF) and granulocytes (G-CSF), and nerve growth factor (NGF).
Oshibe
T
T
Department of Veterinary Immunology, College of Agriculture, Osaka Prefecture University, Japan.
Kitamura
I
I
Tanaka
K
K
Baba
T
T
Kodama
H
H
Mukamoto
M
M
Tsuji
S
S
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D004817:Epidermis / Q000196:embryology / Q000276:immunology*; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology; D015603:Keratinocytes / Q000276:immunology*; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000276:immunology; D013154:Spleen / Q000276:immunology
D016207:Cytokines; D007376:Interleukin-2; D009414:Nerve Growth Factors; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor