Conventional cytogenetics alone is not sufficient for identifying interspecies cell line contamination.
Evidence from our laboratory suggests that conventional chromosome banding analysis alone is not sufficient for detecting interspecies contamination of cell lines. To differentiate noncycling interphase cells of murine or other species origin that may be contaminating a human cell line, molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH) with a total human DNA probe must be used.
Multani
A S
AS
Department of Cancer Biology, University of Texas M. D. Anderson Cancer Center Houston 77030, USA.
Pathak
S
S
D000828:Animal Population Groups / Q000235:genetics; D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000235:genetics / Q000648:ultrastructure; D006224:Cricetinae; D003582:Cytogenetics / Q000379:methods*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000473:pathology; D013045:Species Specificity; D014183:Transplantation, Heterologous
D015342:DNA Probes