Natural antioxidant, chlorogenic acid, protects against DNA breakage caused by monochloramine.
Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with a half inhibition of 67.4 microM. Chlorogenic acid reacted with monochloramine in a time-dependent manner, and the reaction rate increased with decreasing pH. These results suggest that chlorogenic acid prevents genotoxicity of monochloramine in gastric mucosa.
Shibata
H
H
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Japan. shibata@life.shimane-u.ac.jp
Sakamoto
Y
Y
Oka
M
M
Kono
Y
Y
D000643:Ammonium Chloride / Q000737:chemistry; D000975:Antioxidants / Q000494:pharmacology*; D002700:Chloramines / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004269:DNA, Bacterial / Q000737:chemistry / Q000187:drug effects; D016480:Helicobacter pylori / Q000187:drug effects / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D002700:Chloramines; D004269:DNA, Bacterial; D000643:Ammonium Chloride; D002726:Chlorogenic Acid; C030816:chloramine