title: Molecular cloning of newt sex pheromone precursor cDNAs: evidence for the existence of species-specific forms of pheromones.
abstract: Cloning of cDNA encoding a decapeptide pheromone (sodefrin) that attracts conspecific female newts was attempted. A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. This is the first report of a peptide pheromone precursor in vertebrates.
authors.affiliation: Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iwata
authors.forename: K
authors.initials: K
authors.lastname: Umezawa
authors.forename: F
authors.initials: F
authors.lastname: Toyoda
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Matsukawa
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: S
authors.initials: S
authors.lastname: Kikuyama
mesh_terms: D000005:Abdomen / Q000033:anatomy & histology / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000145:classification; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D012455:Salamandridae / Q000235:genetics*; D012724:Sex Attractants / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D012726:Sexual Behavior, Animal; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D009842:Oligopeptides; D011498:Protein Precursors; D012724:Sex Attractants; C092479:sodefrin