Quantification of lipids, liposomes, amino acids, and proteins by thermal ultramicrodigestion and an ultramicrocoulometric assay, based on the reaction of hypobromite with ammonia.
The quantification of nitrogen in organic material is described. It is based on a novel thermal ultramicrodigestion in combination with an ultramicrocoulometric quantification. The lower detection limit of the coulometric measurement is 0.5 microg nitrogen, which corresponds to 20 microg lipid, 3 microg glycine, or 4 microg protein. Therefore it is as sensitive as the frequently used Lowry method. In contrast to the Lowry protein determination it is not disturbed by detergents and most other interfering substances.
Zellmer
S
S
Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Halle, D-06097, Germany.
Kaltenborn
G
G
Rothe
U
U
Lehnich
H
H
Lasch
J
J
Pauer
H D
HD
D000596:Amino Acids / Q000032:analysis*; D000641:Ammonia; D001959:Bromates; D004563:Electrochemistry / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis*; D008081:Liposomes / Q000737:chemistry*; D009584:Nitrogen / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D000596:Amino Acids; D001959:Bromates; D008055:Lipids; D008081:Liposomes; D011506:Proteins; D000641:Ammonia; C027664:hypobromous acid; D009584:Nitrogen