title: Red blood cell metabolism and hemoglobin oxygen affinity. Effect of vinburnine on normobaric hypoxic rats.
abstract: The influence of Vinburnine on red blood cell metabolism and hemoglobin oxygen affinity was investigated in normobaric rats (FiO2: 10%). Two periods of hypoxic exposure were performed (10-21 d). In each experimental series, rats were divided into two groups: Treated rats received a daily intraperitoneal injection of drug (4 mg/kg). Control rats in the same conditions of hypoxia received an isotonic saline solution. For a 10 d exposure period, Vinburnine does not affect red blood cell metabolism nor hemoglobin oxygen affinity. At 21 d period exposure, 2, 3 diphosphoglycerate (2, 3 DPG) and ATP amounts increase in treated rats. The rate of increase was 10% (p<0.05) and 28% (p<0.01), respectively. Red blood cell metabolism effect of Vinburnine was not accompanied by a modification in affinity hemoglobin oxygen (Hb-O2); no statistically significant difference was observed between treated rats and control rats concerning p50 (partial pressure oxygen at half hemoglobin saturation). Results suggest that Vinburnine has a metabolic effect corresponding to a glycolysis anaerobic stimulation, which can improve oxygen delivery to tissues and could explain the favorable hemoreological action of Vinburnine observed in a previous investigation.
authors.affiliation: Laboratoire de physiopathologie hemorespiratoire et enzymatique, Faculté de Médecine et de Pharmacie 2, Besançon, France.
authors.forename: A
authors.initials: A
authors.lastname: Louajri
authors.forename: S
authors.initials: S
authors.lastname: Harraga
authors.forename: G
authors.initials: G
authors.lastname: Toubin
authors.forename: J P
authors.initials: JP
authors.lastname: Kantelip
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D000860:Hypoxia / Q000097:blood*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014748:Vinca Alkaloids / Q000494:pharmacology*
chemical_list: D006454:Hemoglobins; D014748:Vinca Alkaloids; C016422:eburnamonine; D010100:Oxygen