Binding reaction of aflatoxin B1 with immunoglobulin G against aflatoxin B1-bovine serum albumin complex.
Polyclonal immunoglobulin G antibodies were raised against aflatoxin B1-bovine serum albumin complex and characterised. Antibodies against the complex were obtained after a single intradermal multiple site injection of water in oil emulsion of the complex into adult female albino rabbits. Equilibrium dialysis and Scatchard plot analysis of the interaction of the antibodies with aflatoxin B1 showed that the antibodies have high affinity for binding aflatoxin B1. The average number of binding sites on the antibody molecules for aflatoxin B1 is 1.74 +/- 0.20 with mean standard free energy change (delta F1(0) of -23.10 KJ/mol, while the average association constant is 2.35 +/- 0.73 x 10(-4)M-1. Male wistar strain albino rats after immunization with the complex showed lower mortality when challenged with a single dose of aflatoxin B1. The results suggest that such antibodies with high affinity for aflatoxin B1 could be used in the immunointerception of the toxin.
Odunola
O A
OA
Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria.
Uwaifo
A O
AO
D016604:Aflatoxin B1 / Q000276:immunology*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology*; D002273:Carcinogens; D004353:Drug Evaluation, Preclinical; D005260:Female; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000936:Antigen-Antibody Complex; D002273:Carcinogens; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D016604:Aflatoxin B1