[Inhibitory effect of linoleyl-hydroxamic acid on the oxidation of linoleic acid by 12-lipoxygenase from porcine leukocytes].
Linoleic acid oxidation by 12-lipoxygenase from porcine leukocytes has been studied as affected by linoleyl-hydroxamic acid. Linoleyl-hydroxamic acid has been found to be an effective inhibitor of porcine leucocyte 12-lipoxygenase. Aerobic preincubation of 12-lipoxygenase with 0.1-6 microM of linoleyl-hydroxamic acid led to a time- and dose-dependent inhibition of the enzyme. The inhibitor's concentration able to induce a 50% loss of the enzyme activity with and without 15-min preincubation were 3.5 and 0.65 microM, respectively. Experimental results obeyed a kinetic scheme, which supposed 2 extra substrate molecules bounding with the enzyme-substrate complex in the presence of linoleyl-hydroxamic acid.
Kharchenko
O V
OV
Institute of Bioorganic Chemistry and Petrolchemistry, National Academy of Sciences of Ukraine, Kyiv.
Cernjuk
V N
VN
Butovich
I A
IA
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000097:blood; D007962:Leukocytes / Q000201:enzymology*; D019787:Linoleic Acid / Q000378:metabolism*; D008041:Linoleic Acids / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D016015:Logistic Models; D010084:Oxidation-Reduction; D013552:Swine
D008041:Linoleic Acids; D016859:Lipoxygenase Inhibitors; C089594:linoleic acid hydroxamate; D019787:Linoleic Acid; D001092:Arachidonate 12-Lipoxygenase