title: The use of honey as an antiseptic in managing Pseudomonas infection.
abstract: A laboratory study was undertaken to extend existing knowledge about the effectiveness of the antibacterial properties of honey against pseudomonads. To date, sensitivity testing has used non-standardised honeys, which may vary greatly in their antibacterial potency. Pure cultures of Pseudomonas spp, isolated from swabs from 20 infected wounds, were inoculated on the surface of nutrient agar plates containing various concentrations of honey in the medium. Two types of honey were used, a manuka honey and a pasture honey, each selected to have antibacterial activity close to the median for each type. The minimum inhibitory concentration of the manuka honey for the 20 isolates ranged from 5.5-8.7% (v/v) (mean 6.9% (v/v), standard deviation 1.3). The minimum inhibitory concentration of the pasture honey for the 20 isolates ranged from 5.8-9.0% (v/v) (mean 7.1% (v/v), standard deviation 1.0). Honeys with an average level of antibacterial activity could be expected to be effective in preventing the growth of pseudomonads on the surface of a wound even if the honey were diluted more than ten-fold by exudation from the wound.
authors.affiliation: School of Biomedical Sciences, University of Wales Institute, Cardiff, UK.
authors.forename: R
authors.initials: R
authors.lastname: Cooper
authors.forename: P
authors.initials: P
authors.lastname: Molan
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D006722:Honey / Q000032:analysis / Q000145:classification; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D000891:Anti-Infective Agents, Local; D006861:Hydrogen Peroxide