title: Nitric oxide synthase activity and Doppler parameters in the fetoplacental and uteroplacental circulation in preeclampsia.
abstract: OBJECTIVE
  We investigated the hypothesis that changes in blood flow in the uteroplacental and fetoplacental circulation in preeclampsia are associated with an abnormality of placental or uterine placental bed nitric oxide (NO) synthesis.
  
  
  METHODS
  We measured pulsatility indices on Doppler waveform analysis from uterine and umbilical arteries in 20 patients with preeclampsia and 14 healthy pregnant controls before elective cesarean section. During cesarean section, biopsies from the uterine placental bed and the placenta were taken and the nitric oxide synthase (NOS) activity was measured by the [3H] L-arginine-[3H] L-citrulline conversion assay in these samples.
  
  
  RESULTS
  The NOS activity was significantly lower in the uterine placental bed in comparison to the placental tissue (p < 0.01). Placental NOS activity was similar between patients with preeclampsia and healthy controls and in the groups with either a pathological or a normal Doppler flow in the umbilical artery. In the uterine placental bed however, NOS activity from patients with preeclampsia was significantly lower (p < 0.01), whereas the blood flow resistance in the uterine arteries was elevated (p < 0.01) in comparison to healthy controls.
  
  
  CONCLUSIONS
  Our data show that pathological Doppler waveforms in the uterine arteries of patients with preeclampsia are paralleled by diminished NOS activity in the uterine placental bed. Therefore, the compromised NO production in the uterine placental bed may play an important role in the impaired uteroplacental blood flow and potentially in some pathological features of preeclampsia such as intervillous thrombosis formation and fetal growth retardation.
authors.affiliation: Department of Obstetrics, University Hospital of Erlangen/Nuremberg, Germany. ernst.beinder@frauen.med.uni-erlangen.de
authors.forename: E
authors.initials: E
authors.lastname: Beinder
authors.forename: M G
authors.initials: MG
authors.lastname: Mohaupt
authors.forename: D
authors.initials: D
authors.lastname: Schlembach
authors.forename: T
authors.initials: T
authors.lastname: Fischer
authors.forename: R B
authors.initials: RB
authors.lastname: Sterzel
authors.forename: N
authors.initials: N
authors.lastname: Lang
authors.forename: C
authors.initials: C
authors.lastname: Baylis
mesh_terms: D000328:Adult; D001783:Blood Flow Velocity; D005260:Female; D005333:Fetus / Q000098:blood supply* / Q000201:enzymology; D006801:Humans; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D010920:Placenta / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000201:enzymology; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D011673:Pulsatile Flow; D018709:Statistics, Nonparametric; D016216:Ultrasonography, Prenatal; D014469:Umbilical Arteries / Q000000981:diagnostic imaging / Q000503:physiopathology; D014599:Uterus / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000201:enzymology
chemical_list: D019001:Nitric Oxide Synthase