Clay-nucleic acid complexes: characteristics and implications for the preservation of genetic material in primeval habitats.
The equilibrium adsorption of three nucleic acids: chromosomal DNA, supercoiled plasmid DNA, and 25S rRNA, on the clay minerals, montmorillonite (M) and kaolinite (K), were studied. Adsorption of the nucleic acid on the clays was rapid and maximal after 90 min of contact time. Chromosomal DNA was adsorbed to a greater extent than plasmid DNA and RNA, and the adsorption was also greater on M than on K. Adsorption isotherms were of the L type, and a plateau was reached with all the complexes, with the exception of chromosomal DNA adsorbed on M. To deterine where nucleic acids are adsorbed on clay minerals and the nature of the interaction, complexes were studied by X-ray diffraction (X-RD), electron microscopy, and Fourier transform infrared (FT-IR) spectroscopy. X-RD showed that nucleic acids did not penetrate the clay, indicating that the adsorption occurred primarily on the external surfaces of clay particles, as also suggested by electron microscopy observations. FT-IR spectra of clay-tightly bound nucleic acid complexes showed adsorption bands that indicate a variation of the nucleic acids status as a consequence of their adsorption on clay. Data obtained suggested that the formation of clay-nucleic acid complex could have an important role in the preservation of genetic material in primeval habitats.
Franchi
M
M
Department of Animal Biology and Genetics, University of Florence, Italy.
Bramanti
E
E
Bonzi
L M
LM
Orioli
P L
PL
Vettori
C
C
Gallori
E
E
D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D001412:Bacillus subtilis / Q000235:genetics; D001546:Bentonite / Q000737:chemistry; D000077215:Clay; D004247:DNA / Q000737:chemistry; D007616:Kaolin / Q000737:chemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009696:Nucleic Acids / Q000737:chemistry* / Q000648:ultrastructure; D012335:RNA, Ribosomal / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D000538:Aluminum Silicates; D009696:Nucleic Acids; D012335:RNA, Ribosomal; D001546:Bentonite; C065039:RNA, ribosomal, 25S; D007616:Kaolin; D004247:DNA; D000077215:Clay