Ultrastructural identification of caveolae and immunocytochemical as well as biochemical detection of caveolin in chondrocytes.
Using fluorescence immunocytochemistry, transmission electron microscopy and Western blotting, we have shown that caveolae and caveolin are abundant on chondrocytes of different cartilaginous structures of newborn and adult rat knee joints. Caveolin was detected in chondrocytes of the outer layer of articular cartilage, in the fibrocartilage of the menisci, and in fibrocartilage-like cells at tendon and ligament insertions. Electron microscopical studies revealed caveolae-like invaginations along the plasmalemmal membrane of articular chondrocytes and fibrocartilage cells. Immunoblot analysis demonstrated caveolin in detergent-insoluble and soluble complexes isolated from cultured rat chondrocytes.
Schwab
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W
Institute of Anatomy, Medical Faculty Carl Gustav Carus, Dresden University of Technology, Germany.
Hempel
U
U
Funk
R H
RH
Kasper
M
M
D000367:Age Factors; D000818:Animals; D000906:Antibodies; D051242:Caveolin 1; D022461:Caveolins; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002478:Cells, Cultured; D019902:Chondrocytes / Q000737:chemistry* / Q000648:ultrastructure*; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007719:Knee Joint / Q000033:anatomy & histology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; C495537:Cav1 protein, rat; D051242:Caveolin 1; D022461:Caveolins; D008565:Membrane Proteins