[The C(27)-steroid hormones ecdysterone and calcitriol activate the phosphoinositol cycle in its membrane phase].
We have examined in vivo the capacities of two C27-steroid hormones-phytoecdysteroid ecdysterone (20-hydroxyecdysone, 10(-8) M) and calcitriol (1 alpha, 25-dihydroxyvitamin D3, 10(-12) M), as a modulators of phosphoinositide cycle in the brain and heart cells. Severe lines of evidence indicate that both hormones may acts as positive regulators of phosphoinositide hydrolysis by phospholipases C and D in membrane (0.5-30 min) pre-genomic phase of its actions. Thus, our findings identify ecdysterone, like calcitriol, as a positive regulator of [Ca2+]i, and protein kinase in the mammalian cells.
Kotsiuruba
A V
AV
A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Bukhanevich
O M
OM
Mehed'
O F
OF
Tarakanov
S S
SS
Berdishev
A H
AH
Tuhanova
A V
AV
D000818:Animals; D002117:Calcitriol / Q000502:physiology*; D004441:Ecdysterone / Q000502:physiology*; D006868:Hydrolysis; D008566:Membranes / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism*
D010716:Phosphatidylinositols; D004441:Ecdysterone; D010738:Type C Phospholipases; D010739:Phospholipase D; D002117:Calcitriol