Sugars modulate an unusual mode of control of the cell-wall invertase gene (Incw1) through its 3' untranslated region in a cell suspension culture of maize.
We show here that a cell-wall invertase encoded by the Incw1 gene is regulated at both the transcriptional and posttranscriptional levels by sugars in a heterotrophic cell suspension culture of maize. The Incw1 gene encoded two transcripts: Incw1-S (small) and Incw1-L (large); the size variation was attributable to different lengths in the 3' untranslated region. Both metabolizable and nonmetabolizable sugars induced Incw1-L RNA apparently by default. However, only the metabolizable sugars, sucrose and D-glucose, were associated with the increased steady-state abundance of Incw1-S RNA, the concomitant increased levels of INCW1 protein and enzyme activity, and the downstream metabolic repression of the sucrose synthase gene, Sh1. Conversely, nonmetabolizable sugars, including the two glucose analogs 3-O-methylglucose and 2-deoxyglucose, induced greater steady-state levels of the Incw1-L RNA, but this increase did not lead to either an increase in the levels of the INCW1 protein/enzyme activity or the repression of the Sh1 gene. We conclude that sugar sensing and the induction of the Incw1 gene is independent of the hexokinase pathway. More importantly, our results also suggest that the 3' untranslated region of the Incw1 gene acts as a regulatory sensor of carbon starvation and may constitute a link between sink metabolism and cellular translation in plants.
Cheng
W H
WH
Program in Plant Molecular and Cellular Biology, University of Florida, Gainesville FL, 32611-0680, USA.
Taliercio
E W
EW
Chourey
P S
PS
D020413:3' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D002473:Cell Wall / Q000201:enzymology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004794:Enzyme Repression; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000235:genetics; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013395:Sucrose / Q000494:pharmacology*; D003313:Zea mays / Q000166:cytology / Q000235:genetics*; D043324:beta-Fructofuranosidase
D020413:3' Untranslated Regions; D011994:Recombinant Proteins; D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005947:Glucose