title: Osmoregulation-dependent expression of Yersinia enterocolitica virulence factors.
abstract: The effects of hyperosmotic stress on expression of plasmid coded Yop and Yad A proteins--virulence factors of Y.enterocolitica serotype 0:9 were characterized. When cells were shifted to high osmolarity and cultured at 37 degrees C in medium without Ca2+ the production of Yops was inhibited. In contrast, the amount of Yad A protein was unaffected. Addition of glycine betaine to this culture alleviated the effect of high osmolarity. It was also found that hyperosmotic stress causes increased negative supercoiling of DNA in Y. enterocolitica 0:9. Changes in DNA supercoiling coincided with expression of Yop proteins. These results suggest that in high osmolarity the expression of yop genes may be regulated by DNA supercoiling.
authors.affiliation: Institute of Microbiology, Warsaw University, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Brzostek
authors.forename: I
authors.initials: I
authors.lastname: Stencel
authors.forename: J
authors.initials: J
authors.lastname: Hrebenda
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001622:Betaine; D015153:Blotting, Western; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D010957:Plasmids / Q000235:genetics; D014774:Virulence / Q000235:genetics; D014882:Water-Electrolyte Balance; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D004278:DNA, Superhelical; C064832:YadA protein, Yersinia; D001622:Betaine