Peptide ligation and its application to protein engineering.
The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.
Cotton
G J
GJ
Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Muir
T W
TW
D000818:Animals; D006801:Humans; D008024:Ligands; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D015202:Protein Engineering; D011499:Protein Processing, Post-Translational
D008024:Ligands; D010446:Peptide Fragments; D010455:Peptides