Mutation screening of the interferon-gamma gene as a candidate gene for multiple sclerosis.
Interferon-gamma (IFN-gamma) plays a crucial role in the regulation of the immune response. Alterations in IFN-gamma production have been found in several diseases including multiple sclerosis (MS). Such alterations could be caused by the action of different factors on cytokine production, or, theoretically, by mutations in the gene. We screened the IFN-gamma gene promoter and part of the first intron, known to contain a c-Rel specific enhancer, for possible mutations by sequencing. We found a C to T substitution in the IFN-gamma promoter at position -333. Screening for this mutation by sequence-specific PCR in 214 MS patients and 164 controls identified two patients, both heterozygous, but no controls with this mutation. No mutations were found in the first intron. The interferon-gamma gene is highly conserved and changes in IFN-gamma expression are probably due to the influence of regulatory factors on gene transcription, rather than gene polymorphisms.
Giedraitis
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Division of Neurology, Huddinge University Hospital, Karolinska Institute, Sweden.
He
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Hillert
J
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D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007438:Introns; D009103:Multiple Sclerosis / Q000235:genetics*; D009154:Mutation; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020569:Proto-Oncogene Proteins c-rel; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D007371:Interferon-gamma