Structure and alternative splicing of the gene encoding alpha1I, a human brain T calcium channel alpha1 subunit.
The structure of CACNA1I, the gene encoding alpha1I, a human brain T Ca2+ channel alpha1 subunit, was determined by comparison of polymerase chain reaction-amplified brain cDNA and genomic sequences. The gene consists of at least 36 exons spanning at least 115,168 basepairs of chromosome 22q12.3-13.2. The predicted protein has 2016 amino acids and 28 potential phosphorylation sites. Alternative splicing of the gene occurs at two sites: cassette exon 9 and an alternative acceptor in exon 33. Molecular diversity generated by alternative splicing and post-translational modification of this and other members of the T alpha1 subunit gene family may account for the observed heterogeneity of T currents in central neurons.
Mittman
S
S
Department of Anesthesiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. smittman@jhmi.edu
Guo
J
J
Emerick
M C
MC
Agnew
W S
WS
D017398:Alternative Splicing; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D018076:DNA, Complementary; D005091:Exons; D006031:Glycosylation; D006801:Humans; D007438:Introns; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary
D015220:Calcium Channels; D018076:DNA, Complementary