title: Enantiomeric separation of D- and L-carnitine by integrating on-line derivatization with capillary zone electrophoresis.
abstract: A new capillary zone electrophoretic method has been developed for the enantiomeric separation and quantification of enantiomers of carnitine, D- and L-carnitine were derivatized with 9-fluorenylmethyl chloroformate in a flow system, working on-line with the capillary electrophoretic equipment. The separation was performed using a selective chiral buffer containing 2,6-dimethyl-beta-cyclodextrin (heptakis). Triethanolamine was used as electroosmotic modifier and the separation was carried out in a uncoated capillary. Under the optimal conditions the resolution between D- and L-carnitine was 1.2 and the limits of detection for both isomers were about 5.0 microM. The proposed method was applied to the determination of D-carnitine in excess of L-carnitine in synthetic samples, and the results demonstrated that the maximal D-:L-carnitine ratio determined was approximately 1:100.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Cordoba, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Mardones
authors.forename: A
authors.initials: A
authors.lastname: Ríos
authors.forename: M
authors.initials: M
authors.lastname: Valcárcel
authors.forename: R
authors.initials: R
authors.lastname: Cicciarelli
mesh_terms: D002331:Carnitine / Q000302:isolation & purification*; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004983:Ethanolamines / Q000737:chemistry; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D004983:Ethanolamines; D047392:beta-Cyclodextrins; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; C009546:triethanolamine; D002331:Carnitine