Specifically designed polymeric nanospheres increase cellular uptake of unmodified antisense ODNs.
The cellular uptake and the inhibitory effect of c-myb unmodified antisense oligonucleotides reversibly bound to new polymeric nanoparticles in HL-60 cellular system have been found to increase by 50 folds if compared with the free ODN. An initial single dose (320 nM) of the nanoparticle bound unmodified antimyb ODN has been able to specifically inhibit HL-60 leukemia cell proliferation for at least 8 days.
Tondelli
L
L
I.Co.C.E.A, Consiglio Nazionale delle Ricerche, Bologna, Italy.
Laus
M
M
Ricca
A
A
Citro
G
G
D001483:Base Sequence; D018922:HL-60 Cells; D006801:Humans; D008863:Microspheres; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D011108:Polymers; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators / Q000235:genetics
D016376:Oligonucleotides, Antisense; D011108:Polymers; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators