Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus.
We have investigated the bovine immune response to heterologous proteins expressed using a recombinant rinderpest virus (RPV). A new gene unit was created in a cDNA copy of the genome of the vaccine strain of RPV, and an open reading frame inserted that encodes the polymerase (3Dpol) and parts of the capsid protein VP1 from foot-and-mouth disease virus (FMDV). Infectious recombinant RPV was rescued and shown to express the FMDV-derived protein at good levels in infected cells. The rescued virus was only slightly more attenuated in tissue culture than the original virus. Cattle infected with this recombinant generated a normal immune response to RPV, and were protected from lethal challenge by that virus. Experimental animals showed a specific delayed-type hypersensitivity response to FMDV 3Dpol, similar to that seen in FMDV infection; however, no antibodies were detected recognizing either of the components of the FMDV-derived protein, nor was any proliferative response to these epitopes found in isolated peripheral blood lymphocytes from infected animals. No protection was seen against FMDV infection.
Baron
Michael D
MD
Divisions of Molecular Biology1 and Immunology2, Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Foster-Cuevas
Mildred
M
Divisions of Molecular Biology1 and Immunology2, Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Baron
Jana
J
Divisions of Molecular Biology1 and Immunology2, Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Barrett
Thomas
T
Divisions of Molecular Biology1 and Immunology2, Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012302:Rinderpest virus / Q000235:genetics* / Q000276:immunology; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D018985:Epitopes, B-Lymphocyte; D011993:Recombinant Fusion Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D017361:Viral Nonstructural Proteins; C103557:virus-infection-associated antigen, Foot-and-mouth disease virus; D012321:DNA-Directed RNA Polymerases