Effect of lysophosphatidic acid on the ovum transport in mouse oviducts.
The effects of lysophosphatidic acid (LPA) on ovum transport in mouse oviducts were studied. When excised oviducts were incubated at 37 degrees C under 5% CO2 in humidified air for 24 hours, addition of LPA at 10 microM to the medium significantly accelerated the rate of ovum transport, and 1 microM LPA slightly increased the ovum transport rate. These increases were not inhibited by 10 microM indomethacin, a cyclooxygense inhibitor, but were suppressed by 260 ng/ml of pertussis toxin or 10 microM verapamil, a voltage-sensitive calcium channel blocker. These data suggested that LPA stimulates mouse ovum transport by contracting oviductual smooth muscle via a voltage-sensitive calcium channel mediated by a pertussis toxin-sensitive G-protein-linked receptor.
Kunikata
K
K
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Japan.
Yamano
S
S
Tokumura
A
A
Aono
T
T
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D005187:Fallopian Tubes / Q000187:drug effects*; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008246:Lysophospholipids / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010066:Ovum Transport / Q000187:drug effects*; D037342:Pertussis Toxin; D014700:Verapamil / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002121:Calcium Channel Blockers; D008246:Lysophospholipids; D010566:Virulence Factors, Bordetella; D014700:Verapamil; D037342:Pertussis Toxin; D007213:Indomethacin