Protective effect of the yeast glucomannan against cyclophosphamide-induced mutagenicity.
Glucomannan (GM) isolated from Candida utilis with molecular weight 30 kDa was administered either intraperitoneally or orally prior to cyclophosphamide (CP) injection and its effect on the frequency of micronuclei was evaluated in polychromatic erythrocytes of mouse bone marrow. GM administration by either route decreased significantly (p<0.002) the clastogenic effect of CP. The protective effect was concentration-dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg b. wt. (body weight). The fact that GM was effective also at oral administration is indicative of the passage of GM molecules through the wall of the gastrointestinal tract. The important characteristics of GM isolated from C. utilis, such as good water solubility, relatively small molecular weight (30 kDa), and antimutagenic effect exerted also at oral administration, appear to be promising features for its prospective use as a natural protective agent.
Chorvatovicová
D
D
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 16, Bratislava, Slovak Republic.
Machová
E
E
Sandula
J
J
Kogan
G
G
D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects; D002175:Candida; D003520:Cyclophosphamide / Q000633:toxicity*; D005260:Female; D008351:Mannans / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D008970:Molecular Weight; D009153:Mutagens / Q000633:toxicity*
D016587:Antimutagenic Agents; D008351:Mannans; D009153:Mutagens; C022901:(1-6)-alpha-glucomannan; D003520:Cyclophosphamide