Age-related sensitivity to cisplatin ototoxicity in gerbils.
This study was undertaken to define the developmental period of maximal sensitivity to cisplatin ototoxicity in gerbils. Five groups were established based upon post-natal age (P) at exposure to cisplatin, P10 (n = 8), P14 (n = 8), P18 (n = 6), P22 (n = 7) and P42 (n = 7). Animals were given cisplatin, 1 mg/kg/day intraperitoneal for 4 days. In the first four groups, P10, P14, P18 and P22, distortion product otoacoustic emissions were measured at 45 days of age, when responses were expected to be developmentally stable. Distortion product grams and input-output functions were measured. There was a statistically significant difference only between P14 and P42 (P<0.01). There was a significant interaction of age and frequency in the P14 group only (P<0.01). A secondary analysis compared distortion product grams of P14 animals, exposed to cisplatin, and age-matched saline-treated animals (n = 6). There was a significant treatment effect. In summary, there was an effect of age on the cisplatin ototoxicity in gerbils. Also, there was an effect of the frequency on DPOAE levels in P14 gerbils. These data support the presence of a 'sensitive' period to cisplatin ototoxicity in gerbils.
Sie
K C
KC
Children's Hospital and Regional Medical Center, and Department of Otolaryngology-Head and Neck Surgery, University of Washington School of Medicine, Seattle 98105, USA. ksie@chmc.org
deSerres
L M
LM
Norton
S J
SJ
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000502:physiology*; D000970:Antineoplastic Agents / Q000506:poisoning*; D002945:Cisplatin / Q000506:poisoning*; D007758:Ear, Inner / Q000187:drug effects*; D005849:Gerbillinae; D007274:Injections, Intraperitoneal; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects
D000970:Antineoplastic Agents; D002945:Cisplatin