Sandwich enzyme-linked immunosorbent assay for plasma cholesteryl ester transfer protein concentration.
OBJECTIVE
Cholesteryl ester transfer protein (CETP) mediates the transfer of HDL cholesterol to apoB-containing lipoproteins. Its mass and activity are increased in several pro-atherogenic conditions. The objective of this study is to develop a cost- and time-effective sandwich ELISA for plasma CETP concentration.


METHODS
Monoclonal anti-CETP, TP20, was used as the capture antibody, while the other biotinylated monoclonal anti-CETP, TP2, was used for detection. The results were expressed in an arbitrary unit, ng biotin-TP2 bound per microl plasma. Plasma CETP concentrations, activities and their relationship were assessed in 35 IDDM children.


RESULTS
The assay had an intra-assay CV of 8.75% and an inter-assay CV under 10%. Plasma CETP concentration of these subjects ranged from 0.36-1.89 ng biotin-TP2/microL. CETP concentration was significantly correlated with CETP activity (r = 0.51, p < 0.01).


CONCLUSIONS
The sandwich ELISA we have developed carried sufficient sensitivity for assaying plasma CETP concentration in human.
Chang
C K
CK
OSU Nutrition Program, Ohio State University, Columbus, USA.
Tso
T K
TK
Snook
J T
JT
Zipf
W B
WB
Lozano
R A
RA
D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000097:blood* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D053480:Cholesterol Ester Transfer Proteins; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D005260:Female; D006023:Glycoproteins; D006801:Humans; D008297:Male; D012015:Reference Standards
D000911:Antibodies, Monoclonal; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins