On-line monitoring of gene expression.
Gene expression in cultures of Escherichia coli has been determined in situ and on-line by the use of an electrochemical sensor. Intact bacteria were used to monitor the induction of the lacZ gene; the onset of stationary phase was also monitored, using a reporter gene fused to the RpoS-dependent promoter of the osmY gene. The technique described can in principle be used to determine the activity of any promoter, with a variety of reporter genes. This technology is non-intrusive, allows real-time monitoring of gene expression, and will be useful in the study of growth regulation and development.
Biran
Israel
I
Klimentiy
Levcov
L
Hengge-Aronis
Regine
R
Ron
Eliora Z
EZ
Rishpon
Judith
J
D015374:Biosensing Techniques; D004563:Electrochemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D007763:Lac Operon; D009862:Online Systems; D012984:Software; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D001616:beta-Galactosidase