title: Capacitation induces tyrosine phosphorylation of proteins in the boar sperm plasma membrane.
abstract: Capacitation (activation) of mammalian spermatozoa is accompanied by protein phosphorylation, elevation of the intracellular calcium concentration and an increased plasma membrane fluidity. The subcellular localization of tyrosine phosphorylation during capacitation have not yet been elucidated. The aim of this study was to investigate whether boar sperm capacitation induces tyrosine phosphorylation of plasma membrane proteins. Capacitation induced tyrosine phosphorylation of 3 proteins (27, 37, and 40 kDa), which coincided with an increase in the plasma membrane fluidity. The importance of the induced tyrosine phosphorylation in sperm binding to the zona pellucida and the induction of the acrosome reaction is discussed.
authors.affiliation: Graduate School of Animal Health, Utrecht University, Utrecht, 3508 TD, The Netherlands.
authors.forename: F M
authors.initials: FM
authors.lastname: Flesch
authors.forename: B
authors.initials: B
authors.lastname: Colenbrander
authors.forename: L M
authors.initials: LM
authors.lastname: van Golde
authors.forename: B M
authors.initials: BM
authors.lastname: Gadella
mesh_terms: D020101:Acrosome Reaction; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D004542:Ejaculation; D007700:Kinetics; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019000:Phosphotyrosine / Q000378:metabolism*; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine
chemical_list: D010750:Phosphoproteins; D019000:Phosphotyrosine; D000001:Calcimycin