title: Problems in predicting cell damage from bubble bursting.
abstract: The question is addressed as to whether observed parameter(s) characterizing single bubble burst (bubble jet height and speed) can be used to predict cell damage in sparged animal cell cultures. Bubble burst profiles are examined in the presence of realistic concentrations of fetal calf serum (FCS) or Pluronic F-68 using a high-speed video technique. The damage to TBC3 hybridoma cells from bubble sparging, characterized as a first-order decline, is reduced by even very small concentrations of both FCS and Pluronic F-68, but neither single bubble burst parameters nor surface properties give usable correlations with death rate constants. &copy 1999 John Wiley & Sons, Inc.
authors.affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: D
authors.initials: D
authors.lastname: Dey
authors.forename: A N
authors.initials: AN
authors.lastname: Emery
mesh_terms: D019149:Bioreactors; D002452:Cell Count; D016923:Cell Death; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods*; D018715:Microscopy, Video; D010100:Oxygen / Q000494:pharmacology*; D020442:Poloxamer / Q000494:pharmacology; D013499:Surface Properties; D013500:Surface Tension; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D013501:Surface-Active Agents; D020442:Poloxamer; D010100:Oxygen