Chip-based genotyping by mass spectrometry.
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.
Tang
K
K
Sequenom Inc., 11555 Sorrento Valley Road, San Diego, CA 92121, USA. ktang@sequenom.com
Fu
D J
DJ
Julien
D
D
Braun
A
A
Cantor
C R
CR
Köster
H
H
D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D008855:Microscopy, Electron, Scanning / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012825:Silicon; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013388:Succinimides
D003432:Cross-Linking Reagents; D017931:DNA Primers; D013388:Succinimides; C078238:N-succinimidyl-4-((iodoacetyl)amino)benzoate; D004247:DNA; D012825:Silicon