Gluconeogenesis activation after intravenous angiotensin II in freely moving rats.
Intravenous (IV) administration of angiotensin II (0.95 nmol/100 g body weight) produced a marked increase in plasma glucose of 20 h fasted rats. To investigate the possibility of a stimulation of gluconeogenesis, conscious unrestrained rats were continuously infused with [14C]bicarbonate, 60 microl/min (0.18 microCi/min), and label incorporation into circulating glucose was determined before and after angiotensin injection. The rate of 14C incorporation into blood glucose of fed rats increased significantly after angiotensin II administration, a 279% increase after 20 min (P < 0.01). In conclusion, the results of the present study show that the hyperglycemia induced by intravenous (IV) administration of angiotensin II is accompanied by an activation of gluconeogenesis, as evidenced by a rapid and marked increase in the rate of 14CO2 incorporation into circulating glucose.
Coimbra
C C
CC
Department of Physiology and Biophysics, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil. coimbrac@mono.icb.ufmg.br
Garofalo
M A
MA
Foscolo
D R
DR
Xavier
A R
AR
Migliorini
R H
RH
D000804:Angiotensin II / Q000008:administration & dosage*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002250:Carbon Radioisotopes; D005943:Gluconeogenesis / Q000187:drug effects*; D006943:Hyperglycemia / Q000097:blood / Q000139:chemically induced; D007262:Infusions, Intravenous; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D000804:Angiotensin II