title: Hansenula polymorpha Pex1p and Pex6p are peroxisome-associated AAA proteins that functionally and physically interact.
abstract: We have cloned the Hansenula polymorpha PEX1 and PEX6 genes by functional complementation of the corresponding peroxisome-deficient (pex) mutants. The gene products, HpPex1p and HpPex6p, are ATPases which both belong to the AAA protein family. Cells deleted for either gene (Deltapex1 or Deltapex6) were characterized by the presence of small peroxisomal remnants which contained peroxisomal membrane proteins and minor amounts of matrix proteins. The bulk of the matrix proteins, however, resided in the cytosol. In cell fractionation studies HpPex1p and HpPex6p co-sedimented with the peroxisomal membrane protein HpPex3p in both wild-type cells and in Deltapex4, Deltapex8 or Deltapex14 cells. Both proteins are loosely membrane-bound and face the cytosol. Furthermore, HpPex1p and HpPex6p physically and functionally interact in vivo. Overexpression of PEX6 resulted in defects in peroxisomal matrix protein import. By contrast, overexpression of PEX1 was not detrimental to the cells. Interestingly, co-overproduction of HpPex1p rescued the protein import defect caused by HpPex6p overproduction. Overproduced HpPex1p and HpPex6p remained predominantly membrane-bound, but only partially co-localized with the peroxisomal membrane protein HpPex3p. Our data indicate that HpPex1p and HpPex6p function in a protein complex associated with the peroxisomal membrane and that overproduced, mislocalized HpPex6p prevents HpPex1p from reaching its site of activity.
authors.affiliation: Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands. KIELJAKW@Biol.RUG.NL
authors.forename: J A
authors.initials: JA
authors.lastname: Kiel
authors.forename: R E
authors.initials: RE
authors.lastname: Hilbrands
authors.forename: I J
authors.initials: IJ
authors.lastname: van der Klei
authors.forename: S W
authors.initials: SW
authors.lastname: Rasmussen
authors.forename: F A
authors.initials: FA
authors.lastname: Salomons
authors.forename: M
authors.initials: M
authors.lastname: van der Heide
authors.forename: K N
authors.initials: KN
authors.lastname: Faber
authors.forename: J M
authors.initials: JM
authors.lastname: Cregg
authors.forename: M
authors.initials: M
authors.lastname: Veenhuis
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D018274:Electroporation; D007150:Immunohistochemistry; D008830:Microbodies / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D010843:Pichia / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D011817:Rabbits; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000908:Antibodies, Fungal; D017931:DNA Primers; D004271:DNA, Fungal; D000251:Adenosine Triphosphatases