Analysis of a translational enhancer present within the 5'-terminal sequence of the genomic RNA of potato virus S.
When present as a transcript leader the 5' untranslated sequence from the potato virus S genomic RNA molecule enhances translation of a downstream open reading frame both in vitro and in vivo. Translational enhancement was 30-fold in rabbit reticulocyte lysate and 15 fold in wheat germ above translation from a transcript with a synthetic leader. Transient expression experiments using tobacco protoplasts and particle bombardment of leaf tissue resulted in enhancement of fourteen and five-fold, respectively, above translation with a synthetic leader. In stably transformed plants the PVS 5'UTR enhanced translation yield ca. 5-fold compared with a synthetic 5'UTR.
Turner
R L
RL
Botany Department, University of Leicester, U.K.
Glynn
M
M
Taylor
S C
SC
Cheung
M K
MK
Spurr
C
C
Twell
D
D
Foster
G D
GD
D020121:5' Untranslated Regions / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017788:Carlavirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D011198:Solanum tuberosum / Q000821:virology*
D020121:5' Untranslated Regions; D012367:RNA, Viral