title: Exposure to hyperbaric oxygen induces cell cycle perturbation in prostate cancer cells.
abstract: Cell cycle synchronization of tumor cells by exposure to hyperbaric oxygenation (HBO) may increase the efficacy of chemotherapy or radiation by placing cells into a chemosensitive portion of the cycle. The purpose of the current study was to examine oxygen pressure-dependent relationships with respect to the cell cycle in prostate tumor cells in vitro. LNCaP cells were grown in an incubator at 21% O2 and then exposed to 100% oxygen at pressures up to 6 atmospheres (atm) for 1.5 h. Cells were then returned to the incubator and evaluated for DNA content by propidium iodide and new DNA synthesis with a pulse-chase experiment. Cell cycle effects were evaluated by flow cytometry. Exposure to HBO increased the percentage of cells synthesizing new DNA in a dose-dependent fashion: 0 atm, 44%; 6 atm, 65%. Cells that synthesize new DNA accumulate in G2/M as a function of partial pressure of oxygen. These results suggest that HBO induces cells to enter the cell cycle and accumulate in G2/M. Cell cycle synchronization and entry of senescent cells into the cell cycle suggest that HBO may be a useful adjuvant to chemotherapy or radiation in the treatment of prostate cancer. There are two potential mechanisms of action that may make HBO efficacious in the treatment of prostate cancer. HBO may potentiate cancer chemotherapeutic agents that cause damage to DNA during DNA synthesis or HBO may inhibit cell division causing accumulation in G2/M.
authors.affiliation: Davis Hyperbaric Laboratory, Brooks Air Force Base, San Antonio, Texas 78235-5119, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Kalns
authors.forename: E H
authors.initials: EH
authors.lastname: Piepmeier
mesh_terms: D001274:Atmospheric Pressure; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D016195:G2 Phase; D006801:Humans; D008297:Male; D008938:Mitosis; D010100:Oxygen / Q000008:administration & dosage*; D011419:Propidium; D011471:Prostatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D011419:Propidium; D010100:Oxygen