Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: a rescue pathway for maintaining redox balance.
Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase. 13C and 1H nuclear magnetic resonance studies using [2-13C]glucose and [2-(13)C]acetate as substrates demonstrated that acetate was exclusively converted to ethanol. This novel pathway provides an alternative route for NAD+ regeneration in the absence of lactate dehydrogenase.
Hols
P
P
Microbial Ingredients Section, NIZO Food Research and Wageningen Centre for Food Science, 6710 BA Ede, The Netherlands. hols@gene.ucl.ac.be
Ramos
A
A
Hugenholtz
J
J
Delcour
J
J
de Vos
W M
WM
Santos
H
H
Kleerebezem
M
M
D000085:Acetates / Q000378:metabolism*; D002247:Carbon Isotopes; D005947:Glucose / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000201:enzymology / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D011522:Protons
D000085:Acetates; D002247:Carbon Isotopes; D011522:Protons; D007770:L-Lactate Dehydrogenase; D005947:Glucose