Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines.
We have shown that Werner syndrome (WRN) fibroblast cell lines are unusually sensitive to the DNA-damaging agent 4-nitroquinoline 1-oxide (4NQO), though not to gamma radiation or to hydrogen peroxide. The fusion of 4NQO-sensitive WRN and 4NQO-resistant control fibroblast cell lines generated proliferating WRN x control cell hybrids that expressed WRN protein and were 4NQO-resistant. These results establish the recessive nature of 4NQO sensitivity in WRN cell lines and provide a cellular assay for WRN protein function.
Prince
P R
PR
University of Washington, Department of Pathology, Seattle 98195-7705, USA.
Ogburn
C E
CE
Moser
M J
MJ
Emond
M J
MJ
Martin
G M
GM
Monnat
R J
RJ
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D000483:Alleles; D015153:Blotting, Western; D002459:Cell Fusion; D002461:Cell Line, Transformed; D002470:Cell Survival; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects*; D005838:Genotype; D006801:Humans; D009153:Mutagens / Q000494:pharmacology*; D010641:Phenotype; D014162:Transfection; D014898:Werner Syndrome / Q000235:genetics*
D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide