title: Looking for HLA-G expression in human tumours.
abstract: Tumour and virus infected cells escape CTLs responses by losing some or all HLA class I molecules. However the NK escape mechanism that uses the HLA-A, -B, and -C tumour deficient variants is unknown. To determine whether HLA-G is expressed on tumour cells and thus favours tumour escape by abolishing NK lysis, we studied HLA-G in a large panel of human tumour tissues and human tumour cell lines of different origin that were previously characterized for HLA-A, -B, and -C expression. We studied HLA-G mRNA transcripts using RT-PCR, and HLA-G1 expression by FACS and immunohistochemical techniques. We found several mRNA transcripts of HLA-G isoforms in most of the samples studied. However, we detected no cell surface expression of HLA-G1 using two specific monoclonal antibodies (mAbs) (87G and 01G). We cannot, however, exclude the possibility that some isoforms other than HLA-G1 may be expressed in some tumours.
authors.affiliation: Departamento de Análisis Clínicos, Hospital Universitario Virgen de las Nieves, Granada, Spain.
authors.forename: L M
authors.initials: LM
authors.lastname: Real
authors.forename: T
authors.initials: T
authors.lastname: Cabrera
authors.forename: J
authors.initials: J
authors.lastname: Canton
authors.forename: R
authors.initials: R
authors.lastname: Oliva
authors.forename: F
authors.initials: F
authors.lastname: Ruiz-Cabello
authors.forename: F
authors.initials: F
authors.lastname: Garrido
mesh_terms: D015870:Gene Expression; D006680:HLA Antigens / Q000096:biosynthesis* / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I