TOP mRNAs are translationally inhibited by a titratable repressor in both wheat germ extract and reticulocyte lysate.
Vertebrate TOP mRNAs contain a 5' terminal oligopyrimidine tract (5' TOP), which is subject to selective translational repression in non-growing cells or in cell-free translation systems. In the present study, we monitored in vitro the effect of increasing amounts of a 16 nucleotides long oligoribonucleotide representing the 5' terminus of mouse ribosomal protein S16 mRNA on the translation of TOP and non-TOP mRNAs. Our results demonstrate that the wild-type sequence (but not its mutant counterparts) derepresses the translation of mRNAs containing 5' TOP motifs, but failed to stimulate the translation of non-TOP mRNAs, even if the latter differed only by a single nucleotide from their 5' TOP-containing counterparts. Similar results have been obtained with both wheat germ extract and rabbit reticulocyte lysate. It appears, therefore, that translational repression of TOP mRNAs is achieved in vitro by the accumulation of a titratable repressor rather than by the loss of an activator and that this repressor recognizes multiple TOP mRNAs with a diverse set of 5' TOP motifs.
Biberman
Y
Y
Department of Biochemistry, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Meyuhas
O
O
D000199:Actins / Q000235:genetics; D000818:Animals; D051379:Mice; D009154:Mutation; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D037121:Plant Lectins; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012156:Reticulocytes / Q000502:physiology*; D014908:Triticum / Q000737:chemistry / Q000235:genetics; D014909:Wheat Germ Agglutinins
D000199:Actins; D009843:Oligoribonucleotides; D010936:Plant Extracts; D037121:Plant Lectins; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D012097:Repressor Proteins; D014909:Wheat Germ Agglutinins