Immunohistochemical evidence for the presence of calbindin containing neurones in the myenteric plexus of the guinea-pig stomach.
Using immunohistochemistry we studied the presence of calbindin in myenteric neurones of the guinea-pig stomach. A rabbit anti recombinant rat calbindin-D28k (CALB) stained 12, 12 and 25% of all myenteric neurones in the fundus, corpus and antrum, respectively. A rabbit anti recombinant human CALB stained 4, 4 and 16%, respectively. A mouse monoclonal antibody against the chicken intestinal CALB showed no labelling. In all regions most calbindin neurones were additionally choline acetyltransferase (ChAT) positive while only a small proportion exhibited nicotinamide adenosine dinucleatide phosphate (NADPH)-diaphorase-activity. Numerous calbindin-positive varicose nerve fibres were present within myenteric ganglia, rarely detectable in the muscle layers and virtually absent in the mucosa. This study demonstrated that a supopulation of cholinergic myenteric neurones in the stomach contain calbindin and suggested that many of these neurones fulfil interneuronal tasks.
Reiche
D
D
Department of Physiology, School of Veterinary Medicine, Hannover, Germany.
Pfannkuche
H
H
Michel
K
K
Hoppe
S
S
Schemann
M
M
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002645:Chickens; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013270:Stomach / Q000294:innervation*
C576498:CALB1 protein, human; C576499:Calb1 protein, mouse; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D009252:NADPH Dehydrogenase; D002795:Choline O-Acetyltransferase