title: On-line monitoring of gene expression.
abstract: Gene expression in cultures of Escherichia coli has been determined in situ and on-line by the use of an electrochemical sensor. Intact bacteria were used to monitor the induction of the lacZ gene; the onset of stationary phase was also monitored, using a reporter gene fused to the RpoS-dependent promoter of the osmY gene. The technique described can in principle be used to determine the activity of any promoter, with a variety of reporter genes. This technology is non-intrusive, allows real-time monitoring of gene expression, and will be useful in the study of growth regulation and development.
authors.forename: Israel
authors.initials: I
authors.lastname: Biran
authors.forename: Levcov
authors.initials: L
authors.lastname: Klimentiy
authors.forename: Regine
authors.initials: R
authors.lastname: Hengge-Aronis
authors.forename: Eliora Z
authors.initials: EZ
authors.lastname: Ron
authors.forename: Judith
authors.initials: J
authors.lastname: Rishpon
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D007763:Lac Operon; D009862:Online Systems; D012984:Software; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase