title: Identification of a third metalloprotease toxin gene in extraintestinal isolates of Bacteroides fragilis.
abstract: To further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.
authors.affiliation: Laboratory of Bacterial Toxins, Department of Microbiology, National Institute of Health, Seoul, Korea.
authors.forename: G T
authors.initials: GT
authors.lastname: Chung
authors.forename: A A
authors.initials: AA
authors.lastname: Franco
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: G E
authors.initials: GE
authors.lastname: Rhie
authors.forename: R
authors.initials: R
authors.lastname: Cheng
authors.forename: H B
authors.initials: HB
authors.lastname: Oh
authors.forename: C L
authors.initials: CL
authors.lastname: Sears
mesh_terms: D000595:Amino Acid Sequence; D001442:Bacteroides Infections / Q000382:microbiology; D001441:Bacteroides fragilis / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007723:Korea; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D004269:DNA, Bacterial; D007527:Isoenzymes; D008666:Metalloendopeptidases; C091198:fragilysin