R-esp1, a rat homologue of drosophila groucho, is differentially expressed after optic nerve crush and mediates NGF-induced survival of PC12 cells.
The differential display reverse transcription polymerase chain reaction method was used to detect alterations in gene expression in the superior colliculus after optic nerve crush in adult rats. One of the most prominent changes observed was the selective induction of R-esp1, a homologue of the Drosophila enhancer of split locus (Groucho). Therefore, we studied the influence of R-esp1 on nerve growth factor (NGF)-induced cell survival of PC12 cells. Overexpression of R-esp1 promotes cell survival even in the absence of NGF and, conversely, it is reduced by antisense-mediated inhibition of R-esp1 expression. In conclusion, we propose a novel model in which R-esp1 protein mediates the NGF-signaling pathway.
Arndt
M
M
Institute of Immunology, Otto von Guericke University, Leipziger Strasse 44, D-39120, Magdeburg, Germany.
Bank
U
U
Frank
K
K
Sabel
B A
BA
Ansorge
S
S
Lendeckel
U
U
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004330:Drosophila / Q000378:metabolism; D008297:Male; D009414:Nerve Growth Factors / Q000502:physiology; D009900:Optic Nerve / Q000378:metabolism*; D020221:Optic Nerve Injuries; D016716:PC12 Cells; D051381:Rats; D012097:Repressor Proteins / Q000737:chemistry; D015398:Signal Transduction
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D009414:Nerve Growth Factors; D012097:Repressor Proteins; C084531:Tle5 protein, rat; C091023:gro protein, Drosophila