Enhanced inhibition of free radical-induced deoxyribose breakdown by Alzheimer brain homogenates.
The ability of homogenates from Alzheimer and control brains to inhibit formation of thiobarbituric acid reactive products (TBAR) induced by free radicals was compared. The assay for TBAR was modified by adding 1% sodium dodecyl sulfate (SDS) to prevent chromogen adsorption by biological matrices, and by extending the incubation time. The inhibitory activities required smaller equivalents of Alzheimer brain homogenates than control homogenates. Similar inhibitory activities were seen in homogenates from amygdala, temporal cortex and cerebellum. The inhibitory activities were similar in brain homogenates from individuals with different apolipoprotein E status. These results indicate that Alzheimer brain tissue has either increased content of free radical scavengers or is more sensitive to free radical attack than control brains.
Volicer
L
L
E.N. Rogers Memorial Veterans Hospital, Geriatric Research Education Clinical Center, Bedford, MA 01730, USA. volicer.ladislav@bedford.va.gov
Wells
J M
JM
McKee
A C
AC
Kowall
N W
NW
D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D000679:Amygdala / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D003855:Deoxyribose / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D005609:Free Radicals / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D012016:Reference Values; D013702:Temporal Lobe / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001057:Apolipoproteins E; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D003855:Deoxyribose