title: Effects of mannitol and dimethylthiourea on helicobacter pylori-induced IL-8 production in gastric epithelial cells.
abstract: The present study aims at investigating the effects of mannitol and dimethylthiourea, known hydroxyl radical scavengers, on lipid peroxidation as an indicative of oxidative damage, NF-kappa B activation and IL-8 production by Helicobacter pylori in gastric epithelial cells. A human gastric epithelial cell line, AGS, treated with or without mannitol and dimethylthiourea, was incubated in the absence or the presence of H. pylori. As a result, H. pylori significantly stimulated the productions of lipid peroxide and IL-8. Treatment with H. pylori resulted in the activation of two species of NF-kappa B dimers (a p50/p65 heterodimer and a p50 homodimer). Mannitol and dimethylthiourea significantly inhibited lipid peroxide production, NF-kappa B complex formation and IL-8 production by H. pylori. In conclusion, mannitol and dimethylthiourea may attenuate H. pylori-induced gastric inflammation by inhibiting lipid peroxidation and NF-kappa B activation and thereby decreasing IL-8 production.
authors.affiliation: Department of Pharmacology and Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea. Kim626@yumc.yonsei.ac.kr
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: J Y
authors.initials: JY
authors.lastname: Seo
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D004234:Diuretics, Osmotic / Q000494:pharmacology*; D004586:Electrophoresis; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis*; D015227:Lipid Peroxidation / Q000187:drug effects; D008353:Mannitol / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D016207:Cytokines; D004234:Diuretics, Osmotic; D016166:Free Radical Scavengers; D016209:Interleukin-8; D016328:NF-kappa B; D017665:Hydroxyl Radical; D008353:Mannitol; C038983:1,3-dimethylthiourea; D013890:Thiourea