[Cloning of genes associated with taxol-induced apoptosis of human breast cancer cells].
The method of mRNA differential display was employed to isolate expressing genes involved in taxol-induced apoptosis of human breast cancer cells (BCap 37). Results demonstrated that the expressive alteration of twelve clones isolated was associated with taxol-induced apoptosis identified by Northern blot. cDNA sequence of clone C3P3 was highly homologous (99%) to human S-adenosylmethionine (S-AdoMet) synthetase. Increased transcriptional level of clone C3P3 induced by taxol was parallel to enhanced enzyme activity of S-AdoMet synthetase. These results suggested that the expression of these clones isolated by the mRNA differential display be possibly involved in the regulation of taxol-induced apoptosis. Their function in details need further study.
Chen
L
L
Cancer Institute, Zhejiang Medical University, Hangzhou.
Zheng
S
S
Willingham
M C
MC
Fan
W
W
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008716:Methionine Adenosyltransferase / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D018076:DNA, Complementary; D008716:Methionine Adenosyltransferase; D017239:Paclitaxel