Survival of multidrug-resistant Salmonella typhimurium DT104 in egg powders as affected by water activity and temperature.
A study was done to determine if a four-strain mixture of multidrug-resistant Salmonella typhimurium definitive type 104 (DT104) cells and a four-strain mixture of S. typhimurium non-DT104 cells differed in ability to survive in whole egg powder, whole egg powder supplemented with corn syrup solids (38%) and salt (1.9%), egg yolk powder, and egg white powder as affected by a(w)(0.29-0.37 and 0.51-0.61) during storage at 13 or 37 degrees C for 8 weeks. Rates of inactivation of S. typhimurium DT104 and non-DT104 cells were similar within each set of test parameters. With the exception of whole egg powder supplemented with corn syrup solids and salt, death was enhanced at a(w) 0.29-0.37 compared to a(w) 0.51-0.61 when powders were stored at 13 degrees C. Survival of cells in whole egg powder supplemented with corn syrup solids and salt was significantly (P < or = 0.05) higher compared to survival in other egg powders stored at 13 degrees C. The opposite trend occurred in powders at a(w) 0.5-0.61 stored at 37 degrees C. Survival of S. typhimurium DT104 and non-DT104 cells at initial populations of 5.01-5.39 log10 cfu/g of egg white powder containing 4.9, 6.1, or 8.2% moisture at 54 or 82 degrees C for 7 days or 8 h, respectively, was determined. Rates of inactivation of DT104 and non-DT104 cells did not differ. Both cell types were detected in egg white powder containing 4.9% moisture but not in powder containing 8.2% moisture when held at 54 degrees C for 7 days. Heating at 82 degrees C for 8 h failed to eliminate 5 log10 S. typhimurium per g of egg white powder, regardless of the moisture content.
Jung
Y S
YS
Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, University of Georgia, Griffin 30223-1797, USA.
Beuchat
L R
LR
D018432:Drug Resistance, Multiple; D004531:Eggs / Q000382:microbiology*; D005511:Food Handling / Q000379:methods; D012486:Salmonella typhimurium / Q000187:drug effects*; D013696:Temperature; D014867:Water
D014867:Water