Malignant fibrous histiocytomas and H-ras-1 oncogene point mutations.
OBJECTIVE
To investigate the types and the frequencies of H-ras-1 gene mutations in malignant fibrous histiocytomas.


METHODS
Thirty five samples of malignant fibrous histiocytoma tissue were searched for point mutations within "hot spot" codons 12 and 13 of the H-ras-1 oncogene by the specific "nested" polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) and a direct cycle sequencing procedure.


RESULTS
In contrast to previous reports, none of the tumours contained a point mutation or any other changes within or around the hot spot gene sequences.


CONCLUSIONS
These data indicate that H-ras-1 oncogenic activation is not required in the molecular pathway of malignant fibrous histiocytoma formation and cannot be used as a discriminating factor for diagnostic sarcoma typing.
Rieske
P
P
Department of Molecular Biology, Medical University of Lódz, Poland.
Bartkowiak
J
J
Szadowska
A
A
Debiec-Rychter
M
M
D005091:Exons; D011905:Genes, ras; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012983:Soft Tissue Neoplasms / Q000235:genetics*