Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA.
Recently investigators showed that polydnavirus DNA from the parasitic wasp Glyptapanteles indiensis could transform gypsy moth L. dispar cell lines in vitro (McKelvey et al., 1996). Here we show GiPDV DNA is capable of transforming in vitro to varying degrees lepidopteran (IPLB-TN-R2, IPLB-SF-21, IAL-PID2, IPLB-HvT1) and coleopteran (IPLB-DU182E) insect cell lines derived from various somatic tissue types. An insect cell line derived from dipteran Aedes albopictus (C7/10) could not be transformed with G. indiensis polydnavirus.
Gundersen-Rindal
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U.S. Department of Agriculture, Agricultural Research Service, Insect Biocontrol Laboratory, Beltsville, Maryland 20705, USA. dgunders@asrr.arsusda.gov
Lynn
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Dougherty
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D000330:Aedes / Q000166:cytology; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D001517:Coleoptera / Q000166:cytology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D007915:Lepidoptera / Q000166:cytology / Q000821:virology*; D018071:Polydnaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014863:Wasps / Q000821:virology*
D004279:DNA, Viral