Dominance of immunoglobulin G2c in the antiphosphorylcholine response of rats infected with Trichinella spiralis.
The antibody response to the L1 stage of Trichinella spiralis has been described as biphasic. Worms resident in the intestine during the first week of infection stimulate an antibody response against a subset of larval proteins. L1 larvae in the muscle at the end stage of infection stimulate a second antibody response against tyvelose-bearing glycoproteins. Antityvelose antibodies protect rats against challenge infection with larvae. The aim of this study was to characterize the rat B-cell response against larval antigens during the intestinal phase of T. spiralis infection and to test the antiparasitic effects of such antibodies. Strain PVG rats were infected orally with 500 larvae. Antibodies specific for phosphorylcholine-bearing proteins of L1 larvae first appeared in serum 9 days postinfection. Absorption experiments showed that the majority of antilarval antibodies produced in rats 16 days after infection with T. spiralis were specific for phosphorylcholine-bearing proteins. A fraction of these antibodies bound to free phosphorylcholine. Immunoglobulin G2c (IgG2c) producing cells in the mesenteric lymph node dominated this early antibody response. IgG2c is associated with T-independent immune responses in the rat; however, a comparison of athymic rats with euthymic controls suggested that only a small fraction of the phosphorylcholine-related antibody response against T. spiralis was T independent. Phosphorylcholine is a common epitope in antigens of bacteria and nematode parasites and has been shown to be a target of protective immunity in certain bacteria. A monoclonal IgG2c antibody was prepared from infected rats and shown to be specific for phosphorylcholine. Monoclonal phosphorylcholine-specific IgG2c failed to protect rats against intestinal infection with T. spiralis. Therefore, our findings do not support a role for phosphorylcholine-bearing antigens in immune defense against T. spiralis; however, the potency of the immune response induced suggests an immunomodulatory role for the lymphocytes involved.
Peters
P J
PJ
James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Gagliardo
L F
LF
Sabin
E A
EA
Betchen
A B
AB
Ghosh
K
K
Oblak
J B
JB
Appleton
J A
JA
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000276:immunology*; D007132:Immunoglobulin Isotypes; D008297:Male; D051379:Mice; D010767:Phosphorylcholine / Q000276:immunology*; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000276:immunology* / Q000517:prevention & control
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D010767:Phosphorylcholine