title: Partial nucleotide sequencing and molecular evolution of epidemic causing Dengue 2 strains.
abstract: To study the genetic variability and to detect evolutionary changes and movement of dengue 2 (DEN-2) strains, nucleotide sequencing of the envelope protein gene and the nonstructural protein 1 gene junction was performed for 9 isolates from the 1996 Delhi epidemic and 1 isolate from the 1967 Delhi epidemic. The epidemic strains had a divergence of 10%-11% from the 1967 strains, but were quite similar to DEN-2 isolates from Seychelles, Somalia, and Torres Strait. In addition, the sequence data were compared to the prototype DEN-2 strain, New Guinea C, and other published DEN-2 sequences from different parts of the world. The phylogenetic analysis by the Molecular Evolutionary Genetics Analysis program suggests that the 1996 Delhi isolates of DEN-2 were genotype IV. The 1967 isolate was similar to a 1957 isolate of DEN-2, P9-122, from India, and was classified as genotype V. This study indicates that earlier DEN-2 strains of genotype V have been replaced by genotype IV.
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India. urvashib@medinst_ernet_in
authors.forename: U B
authors.initials: UB
authors.lastname: Singh
authors.forename: A
authors.initials: A
authors.lastname: Maitra
authors.forename: S
authors.initials: S
authors.lastname: Broor
authors.forename: A
authors.initials: A
authors.lastname: Rai
authors.forename: S T
authors.initials: ST
authors.lastname: Pasha
authors.forename: P
authors.initials: P
authors.lastname: Seth
mesh_terms: D001483:Base Sequence; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics*; D019143:Evolution, Molecular; D015752:Genes, env; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins