title: Serum obtained from rats after partial hepatectomy enhances growth of cultured colon carcinoma cells.
abstract: Tumour-bearing rats were randomized to a 70% partial hepatectomy or a sham operation. At days 1, 3 or 14, portal and systemic serum was obtained and colon carcinoma cells were cultured in the presence of 5, 10, 20 or 50% serum. Proliferation and epidermal growth factor receptor (EGFr) expression was measured in tumour cells. Proliferation was 25-40% higher in tumour cells cultured with portal serum after hepatectomy than after sham operation when using serum obtained at day 3, but not days 1 and 14 after operation. In cultures with serum obtained at day 14 after operation CC 531 cells showed a 30% higher proliferation rate with systemic hepatectomy serum than CC 531 cells with sham systemic serum. These effects were not mediated by a change in EGFr mRNA and protein levels as the used colon carcinoma cells did not reveal EGFr activity by any of the three detection methods used.
authors.affiliation: Department of Surgery, Division of Hepatobiliary Surgery and Liver Transplantation, University Hospital and Medical Faculty of the University of Groningen, Groningen, The Netherlands. k.p.de.jong@chir.azg.nl
authors.forename: K P
authors.initials: KP
authors.lastname: de Jong
authors.forename: M A
authors.initials: MA
authors.lastname: Brouwers
authors.forename: M L
authors.initials: ML
authors.lastname: van Veen
authors.forename: M
authors.initials: M
authors.lastname: Brinker
authors.forename: E G
authors.initials: EG
authors.lastname: de Vries
authors.forename: T
authors.initials: T
authors.lastname: Daemen
authors.forename: G L
authors.initials: GL
authors.lastname: Scherphof
authors.forename: M J
authors.initials: MJ
authors.lastname: Slooff
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D001685:Biological Factors / Q000097:blood* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D003470:Culture Media / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000378:metabolism; D006325:Heart Atria; D006498:Hepatectomy; D008297:Male; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D011169:Portal Vein; D011184:Postoperative Period; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
chemical_list: D001685:Biological Factors; D003470:Culture Media; D004815:Epidermal Growth Factor