title: Investigation of influence of Proteus mirabilis LPS polysaccharide part on the murine immune cells activation.
abstract: The biological activities were investigated of Proteus mirabilis lipopolysaccharides (LPSs) and their fragments, namely, the induction of nitric oxide (NO) and interleukin 1 (IL-1) synthesis by murine macrophages and the proliferation of murine spleen cells. The O-specific polysaccharide (F1), core oligosaccharide (F2) and lipid A were as effective as intact LPS in stimulating murine macrophages to produce NO. IL-1 synthesis was also induced by all studied types of endotoxins (S, Ra, Re) and partial structures, however F1, F2 and lipid A fractions required the presence of serum. In contrast to LPS, the O-specific polysaccharide, core oligosaccharide and lipid A were not able to induce the blast response of murine non-adherent splenocytes.
authors.affiliation: Microbiology and Virology Center, Polish Academy of Sciences, Lódź, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Klink
authors.forename: C H
authors.initials: CH
authors.lastname: Sonn
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D008050:Lipid A / Q000633:toxicity; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D011513:Proteus mirabilis / Q000472:pathogenicity*
chemical_list: D007375:Interleukin-1; D008050:Lipid A; D008070:Lipopolysaccharides; D009569:Nitric Oxide