Oligonucleotide-peptide conjugates as potential antisense agents.
Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized peptides to the 2'-position of a selected nucleotide within an otherwise protected oligonucleotide chain attached to a solid support.
Zubin
E M
EM
Chemical Department and A.N. Belozersky Institute of Physical Chemical Biology, M.V. Lomonosov Moscow State University, Russia.
Romanova
E A
EA
Volkov
E M
EM
Tashlitsky
V N
VN
Korshunova
G A
GA
Shabarova
Z A
ZA
Oretskaya
T S
TS
D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry; D001087:Arabinonucleosides / Q000737:chemistry; D001671:Biochemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D009698:Nucleoproteins / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C120908:2'-amino-2'-deoxyarabinoadenosine; D001087:Arabinonucleosides; D009698:Nucleoproteins; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D010455:Peptides; D000241:Adenosine