Developmental regulation and ultrastructure of glycogen deposits during murine tooth morphogenesis.
The distribution and ultrastructure of glycogen deposits were investigated in the murine tooth germ by histochemical periodic acid-Schiff (PAS) staining and transmission electron microscopy. Lower and upper first molars were examined in mouse embryos at embryonic days 11.5-17 (E11.5-E17) and in 2-day-old postnatal (P2) mice. The oral and dental epithelia and the mesenchymal cells were generally PAS-positive during tooth morphogenesis. PAS-negative cells were present at E13 in the distal tip of the tooth bud epithelium and in the contacting mesenchyme, and this complete lack of PAS reactivity continued in the dental papilla mesenchyme and inner enamel epithelium during the cap and bell stages. The lack of glycogen deposits in the interacting epithelium and mesenchyme during early morphogenesis may be associated with their demonstrated high signaling activities. Mesenchymal cells in the dental follicle consistently possessed small clusters or large pools of glycogen, which disappeared by P2. Since an intense PAS reaction was seen in mesenchymal cells at future bone sites, the glycogen in the dental follicle cells may be associated with their development into hard-tissue-forming cells. Ultrastructural observation of the enamel organ cells from the cap to early bell stages (E14-E15) revealed the occurrence of glycogen pools, which were associated with the Golgi apparatus and with vesicles having amorphous contents. Glycogen particles were also occasionally present inside vesicles or in the extracellular matrix. These may be associated with the exocytosis of glycosaminoglycan components into extracellular spaces and the formation of the stellate reticulum.
Ohshima
H
H
Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Finland. histoman@dent.niigata-u.ac.jp
Wartiovaara
J
J
Thesleff
I
I
D000818:Animals; D002450:Cell Communication / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D006651:Histocytochemistry; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009024:Morphogenesis; D010503:Periodic Acid-Schiff Reaction; D014070:Tooth / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure
D006003:Glycogen