[Construction of poliovirus cDNA chimera with (NKNDD)n and (NANP)n].
Oligonucleotides encoding one copy of NKNDD, containing a P.f. B epitope NKND, and two copies of CSP repeated B epitope NANP were synthesized. After self-ligation, NKNDD and (NANP)2 were cloned into the intermediate vector pSKMM. Series of clones with different copies of NKNDD and NANP were identified. (NKNDD)n/MM with three, four, five, eight copies of NKNDD and (NANP)n/MM with four, six copies of NANP were chosen. Then (NKNDD)n and (NANP)n of these recombinants were recovered respectively and finally inserted into the N-Ag I site of poliovirus type I Mahoney strain full-length cDNA, which is contained in the expression plasmid pSV202H+. As using semi-quantitative hybridization in identifying tranformants, the procedure in our experiment for cloning different copies of NKNDD and NANP was greatly simplified.
Lin
C
C
Institute of Basic Medical Sciences, CAMS, Beijing.
Yuan
J
J
Tu
Z
Z
Liu
E
E
Qiang
B
B
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D000939:Epitopes; D005822:Genetic Vectors; D017780:Malaria Vaccines; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D017955:Poliovirus / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D000939:Epitopes; D017780:Malaria Vaccines; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan