Construction of a multi RE module: exploitation of mechanochemistry of restriction endonucleases.
We describe the construction of a multi-immobilized restriction endonuclease module (Multi RE module). We demonstrate that the applied mechanical stress enables modulation of enzyme activity and modulation of recognition site selectivity (in oligonucleotides of approximately 200 bp) of immobilized restriction endonucleases. The central module which is consisted of different strips of immobilized restriction endonucleases allows limited digestion of a large DNA sample in a controlled manner as a function of applied mechanical stress on strips. The stress-activity relationship and the effect of repeated cycles of stress and relaxation on the immobilized strips are presented here. &copy 1999 John Wiley & Sons, Inc.
Agarwal
P K
PK
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA.
Bhattacharya
S K
SK
D001426:Bacterial Proteins / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D013379:Substrate Specificity
D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D004247:DNA; D004262:DNA Restriction Enzymes