title: Interleukin-5 involvement in ovalbumin-induced eosinophil infiltration in mouse food-allergy model.
abstract: A number of studies demonstrating the important role of interleukin-5 (IL-5) in eosinophil infiltration were reported. Antigen-induced eosinophil infiltrations to the trachea and skin were inhibited by pretreatment with monoclonal anti-IL-5 antibody. In this study, the role of IL-5 in eosinophil infiltration to the gut by oral challenge in mice is investigated. A marked eosinophil infiltration to the lamina propria was induced by oral challenge with ovalubumin (OVA) in Balb/c mice intraperitoneally sensitized with OVA, and peaked at 6 h after the oral challenge. Intraperitoneal preadministration of monoclonal anti-IL-5 antibody significantly decreased the eosinophil infiltration to the lamina propria. Furthermore, analysis by reverse transcription polymerase chain reaction (RT-PCR) demonstrated that IL-5 mRNA expression was induced in the lamina propria in an antigen-specific manner and the expression peaked at 6 h and declined thereafter. In-situ hybridization (ISH) revealed the presence of IL-5 mRNA positive cells at lesion site. As in bronchial mucosa and skin, IL-5 may play an important role in eosinophil recruitment to the lesion site in IgE mediated gut late phase reaction.
authors.affiliation: Department of Dermatology, Nagasaki University School of Medicine, Japan.
authors.forename: S J
authors.initials: SJ
authors.lastname: Bae
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Hakugawa
authors.forename: I
authors.initials: I
authors.lastname: Katayama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002465:Cell Movement / Q000276:immunology*; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D004487:Edema / Q000276:immunology / Q000473:pathology; D004804:Eosinophils / Q000276:immunology* / Q000473:pathology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000473:pathology; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D007413:Intestinal Mucosa / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D002631:Chemotactic Factors, Eosinophil; D015848:Interleukin-5; D012333:RNA, Messenger; D010047:Ovalbumin