Clinical analysis and parasite genetic diversity in human immunodeficiency virus/Chagas' disease coinfections in Brazil.
To evaluate the possible role of parasitemia on Chagas' disease reactivation in Chagas' disease/human immunodeficiency virus (HIV) coinfection cases and the impact of HIV coinfection on Trypanosoma cruzi genetic diversity, 71 patients with Chagas' disease (34 HIV+ and 37 HIV-) were surveyed. Moreover, 92 T. cruzi stocks from 47 chronic chagasic patients (29 HIV+ and 18 HIV-) were isolated and analyzed by multilocus enzyme electrophoresis and a random amplified polymorphic DNA procedure. High parasitemia appeared to play a major role in cases of Chagas' disease reactivation. In HIV+ patients, the genetic diversity and population structure (clonality) of T. cruzi was similar to that previously observed in HIV- patients, which indicates that immunodepression does not modify drastically genotype repartition of the parasite. There was no apparent association between given T. cruzi genotypes and specific clinical forms of Chagas' disease/HIV associations.
Perez-Ramirez
L
L
Centre d'Etudes sur le Polymorphismes des Microorganismes, Unite Mixte de Recherche, Centre National de la Recherche Scientifique/Institut Français de Recherche Scientifique pour le Développement en Coopération, Montpellier, France.
Barnabé
C
C
Sartori
A M
AM
Ferreira
M S
MS
Tolezano
J E
JE
Nunes
E V
EV
Burgarelli
M K
MK
Silva
A C
AC
Shikanai-Yasuda
M A
MA
Lima
J N
JN
Da-Cruz
A M
AM
Oliveira
O C
OC
Guilherme
C
C
Bastrenta
B
B
Tibayrenc
M
M
D000328:Adult; D000368:Aged; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000150:complications / Q000453:epidemiology / Q000469:parasitology*; D004588:Electrophoresis, Cellulose Acetate; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology / Q000469:parasitology*; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification; D008875:Middle Aged; D019105:Random Amplified Polymorphic DNA Technique; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D007527:Isoenzymes