A hookworm allergen which strongly resembles calreticulin.
Immmoglobulin E-rich plasma from patients from Papua New Guinea infected with Necator americanus has been used to probe an adult N. americanus cDNA library for the presence of hookworm allergens. Using this approach, one hookworm allergen has been identified as calreticulin, which was subsequently expressed in Escherichia coli. Little serological cross reactivity was seen between the recombinant calreticulins of this hookworm and its host. Prospective roles for hookworm calreticulin in the host-parasite relationship are discussed in depth.
Pritchard
D I
DI
School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
Brown
A
A
Kasper
G
G
McElroy
P
P
Loukas
A
A
Hewitt
C
C
Berry
C
C
Füllkrug
R
R
Beck
E
E
D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D037282:Calreticulin; D003001:Cloning, Molecular; D003429:Cross Reactions / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D006790:Host-Parasite Interactions / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D009332:Necatoriasis / Q000097:blood / Q000276:immunology / Q000469:parasitology; D010219:Papua New Guinea; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D007073:Immunoglobulin E