Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins.
The deduced sequences of the Omp10, Omp16, and Omp19 outer membrane proteins of Brucella spp. contain a potential bacterial lipoprotein processing sequence. After extraction with Triton X-114, these three proteins partitioned into the detergent phase. Processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. The three proteins were radioimmunoprecipitated from [(3)H]palmitic acid-labeled Brucella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins.
Tibor
A
A
Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium. anne.tibor@fundp.ac.bc
Decelle
B
B
Letesson
J J
JJ
D000215:Acylation; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D002002:Brucella / Q000235:genetics; D002003:Brucella abortus; D004269:DNA, Bacterial; D003902:Detergents; D008074:Lipoproteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D017830:Octoxynol; D010455:Peptides; D011092:Polyethylene Glycols; D011499:Protein Processing, Post-Translational
D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D003902:Detergents; D008074:Lipoproteins; C097318:OMP10 protein, Brucella melitensis biovar Abortus; C097319:OMP19 protein, Brucella abortus; D010455:Peptides; D011092:Polyethylene Glycols; C017339:globomycin; D017830:Octoxynol; C010615:Nonidet P-40