Mechanism and specificity of RNA cleavage by chemical ribonucleases.
Cleaving of model RNA substrates by chemical ribonucleases constructed by conjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine was investigated. Similarly to RNase A, the chemical RNases produce fragments with 5' hydroxy-group and 3'-cyclophosphate. The cleavage occurs as the catalytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved by one molecule of RNase mimic.
Beloglazova
N
N
Institute of Bioorganic Chemistry, Novosibirsk, Russia.
Vlassov
A
A
Konevetc
D
D
Sil'nikov
V
V
Zenkova
M
M
Giege
R
R
Vlassov
V
V
D001483:Base Sequence; D002384:Catalysis; D006868:Hydrolysis; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000235:genetics
C031540:M-protein, influenza virus; D014763:Viral Matrix Proteins; D012313:RNA; D012260:Ribonucleases