title: Cytotoxicity against human leukemic cell lines, and the activity on the expression of resistance genes of flavonoids from Platanus orientalis.
abstract: The cytotoxic activity of three flavonoids, belonging to the kaempherol series, was evaluated against 15 human leukemic cell lines. Flavonoids bearing acyl substituants, 2 and 3, were found to be the most active compounds. A further compound, 1, was examined for its ability to modulate the expression of MDR-1 and GST-pi resistance genes and compounds 2 and 3 for their effect on the uptake of [3H]-thymidine as a marker of DNA synthesis.
authors.affiliation: University of Athens, Department of Pharmacy, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Mitrocotsa
authors.forename: S
authors.initials: S
authors.lastname: Bosch
authors.forename: S
authors.initials: S
authors.lastname: Mitaku
authors.forename: C
authors.initials: C
authors.lastname: Dimas
authors.forename: A L
authors.initials: AL
authors.lastname: Skaltsounis
authors.forename: C
authors.initials: C
authors.lastname: Harvala
authors.forename: G
authors.initials: G
authors.lastname: Briand
authors.forename: C
authors.initials: C
authors.lastname: Roussakis
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002051:Burkitt Lymphoma / Q000473:pathology; D004261:DNA Replication / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D020014:K562 Cells / Q000187:drug effects; D007938:Leukemia / Q000473:pathology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D007527:Isoenzymes; D009363:Neoplasm Proteins; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase