title: Regulation of Entamoeba invadens encystation and gene expression with galactose and N-acetylglucosamine.
abstract: Encystation of Entamoeba invadens parasites is prevented by the presence of free galactose or N-acetylglucosamine in the encystation medium. Galactose prevents the formation of amoeba cellular aggregates which develop during the early phase of encystation, suggesting the presence of functional cell surface galactose-binding molecules, whereas N-acetylglucosamine allows aggregation to occur and prevents cyst formation at a later point. While studying sugar inhibition of amoeba encystation, it was found that high efficiency encystation required the inclusion in encystation medium of precise amounts of polyvalent galactose-terminated molecules, and these molecules could be supplied by serum or by defined glycoconjugates, including mucin. Addition of free galactose to encystation medium prevented the accumulation of three transcripts which are normally upregulated during encystation, and N-acetylglucosamine prevented accumulation of one of the transcripts. These results suggest the presence of distinct sugar-sensitive pathways that regulate differentiation of the amoeba trophozoite into infectious cysts.
authors.affiliation: Department of Medical and Molecular Parasitology, New York University, School of Medicine, NY 10010, USA.
authors.forename: A
authors.initials: A
authors.lastname: Coppi
authors.forename: D
authors.initials: D
authors.lastname: Eichinger
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D000818:Animals; D001769:Blood; D015152:Blotting, Northern; D003470:Culture Media / Q000737:chemistry; D004747:Entamoeba / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology*; D005786:Gene Expression Regulation; D009077:Mucins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D009077:Mucins; D000117:Acetylglucosamine; D005690:Galactose