A comprehensive analysis of heat shock protein synthesis in human peripheral lymphocytes: the effect of penicillin/streptomycin.
A reliable experimental procedure is described for the simultaneous characterisation of a comprehensive range of heat shock proteins (hsps) in human peripheral lymphocytes. In this system, a mild heat shock from 37 to 42 degrees C for 1 h induced the synthesis of hsps 105, 90, 70, 60, 57, 47, 40, 27 and 16. Densitometric analyses of 35[S]-methionine labelled protein gels indicated that levels of these hsps peaked at 3 to 4 h, following post-heat shock recovery at 37 degrees C. The presence of penicillin and streptomycin in the cell culture medium, appeared to have little effect on the kinetics of hsp synthesis. The present method can be used for relatively small blood samples and its relative ease of application and reproducibility make it appropriate for screening the expression of hsps in human lymphocytes from a range of individuals.
Rao
D V
DV
School of Biological Sciences, University of New England, Armidale, NSW, Australia.
Jones
G L
GL
Watson
K
K
D000293:Adolescent; D000328:Adult; D001345:Autoradiography; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010406:Penicillins / Q000494:pharmacology*; D013307:Streptomycin / Q000494:pharmacology*; D013462:Sulfur Radioisotopes; D013696:Temperature
D006360:Heat-Shock Proteins; D010406:Penicillins; D013462:Sulfur Radioisotopes; D013307:Streptomycin