Improved titers for helper virus-free herpes simplex virus type 1 plasmid vectors by optimization of the packaging protocol and addition of noninfectious herpes simplex virus-related particles (previral DNA replication enveloped particles) to the packaging procedure.
A helper virus-free herpes simplex virus type 1 (HSV-1) plasmid vector system has potential for both gene therapy and physiological studies, but relatively low titers have complicated use of this system. In this article, the packaging efficiency was improved by optimizing the packaging protocol and by adding noninfectious HSV-1-related particles, i.e., previral DNA replication enveloped particles (PREPs), during the packaging procedure. PREPs contain many of the tegument proteins that are thought to enhance an HSV-1 infection. Use of both the optimized packaging protocol and the PREPs resulted in an approximately 50-fold increase in the titer, and five different HSV-1 vectors were packaged using this procedure. A purified vector stock (7.8x10(8) infectious vector particles/ml) was microinjected into the striatum, the rats were sacrificed 4 days after gene transfer, and the brains were found to contain an average of approximately 6740 X-Gal-positive striatal cells. This improved packaging procedure may augment use of this vector system.
Sun
M
M
Division of Endocrinology, Children's Hospital, Boston, MA 02115, USA.
Zhang
G R
GR
Yang
T
T
Yu
L
L
Geller
A I
AI
D000818:Animals; D001921:Brain / Q000821:virology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006378:Helper Viruses; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D051381:Rats; D014759:Viral Envelope Proteins / Q000378:metabolism; D014771:Virion / Q000502:physiology; D019065:Virus Assembly; D014779:Virus Replication / Q000502:physiology
D014759:Viral Envelope Proteins