title: Legionella pneumophila invasion of MRC-5 cells induces tyrosine protein phosphorylation.
abstract: After uptake and intracellular multiplication of Legionella pneumophila in MRC-5 lung fibroblasts, important cytoskeletal filament structures, like actin, tubulin, or vimentin, and a cell membrane-associated fibronectin were rearranged during early infection, resulting in a loss of cell adhesion and collapse of the cytoskeleton. Dysregulation of the cellular phosphorylation and dephosphorylation cascade may contribute to the observed changes and may support intracellular survival and multiplication of L. pneumophila. We therefore studied expression of phosphoproteins during intracellular growth of L. pneumophila. By using an anti-tyrosine phosphoprotein antibody we showed that proteins phosphorylated on tyrosine residues accumulated progressively during late infection exclusively around or in phagosomes filled with bacteria. In contrast, expression of serine/threonine phosphoproteins did not change. To discern the origin of phosphorylated proteins, the host cells were treated with cycloheximide, an inhibitor of eukaryotic protein synthesis. The newly synthesized proteins were labeled metabolically with [(35)S]methionine-cysteine and immunoprecipitated with a phosphotyrosine-specific antibody. Sodium dodecyl sulfate gel electrophoresis gave evidence for synthesis of at least three protein clusters (160 to 200, 35 to 60, and 19 to 28 kDa) of Legionella origin that were phosphorylated on tyrosine residues 24 h after infection. Treatment of infected host cells with genistein, a tyrosine kinase inhibitor, revealed that tyrosine protein phosphorylation was not important for bacterial uptake but contributed to intracellular growth of L. pneumophila. Bacterial tyrosine phosphoproteins and the observed intracellular structural changes may be important to understanding the process involved in intracellular growth of L. pneumophila.
authors.affiliation: Department of Medical Microbiology and Hygiene, Institute for Microbiology and Immunology, University of Ulm, Ulm, Germany. milorad.susa@medizin.uni-ulm.de
authors.forename: M
authors.initials: M
authors.lastname: Susa
authors.forename: R
authors.initials: R
authors.lastname: Marre
mesh_terms: D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000382:microbiology; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007424:Intracellular Fluid; D007700:Kinetics; D016952:Legionella pneumophila / Q000254:growth & development / Q000378:metabolism*; D008168:Lung / Q000166:cytology; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors
chemical_list: D004791:Enzyme Inhibitors; D010750:Phosphoproteins; D019000:Phosphotyrosine; D019833:Genistein; D011505:Protein-Tyrosine Kinases