title: Feline peripheral blood mononuclear cells express message for both CXC and CC type chemokine receptors.
abstract: The feline immunodeficiency virus (FIV) causes a disease in cats similar in clinical presentation and disease progression to that of HIV and AIDS. It is now known that, for HIV infection, as well as primary binding of virus to the CD4 receptor, entry and infection of cells requires coreceptors which are members of the chemokine group of G-protein coupled receptors. Because of the similarity of HIV and FIV, we hypothesised that coreceptors are required for the entry and infection of cells by FIV. Using a feline cDNA library derived from a feline IL-2 sensitive lymphocyte cell line, we identified the presence of message for both CC and CXC chemokine receptors. The feline CXCR4 has been shown to facilitate fusion by FIV [44] and we suggest that the feline CCR5 receptor mediates infection of feline cells by M-tropic strains of FIV.
authors.affiliation: Division of Veterinary Pathobiology, School of Veterinary Science, University of Queensland, St. Lucia, Australia.
authors.forename: E M
authors.initials: EM
authors.lastname: Kovacs
authors.forename: G D
authors.initials: GD
authors.lastname: Baxter
authors.forename: W F
authors.initials: WF
authors.lastname: Robinson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D019713:Receptors, CCR5; D019718:Receptors, CXCR4