title: Evaluation of DNA ploidy combined with a cytometric proliferation index of imprints from core needle biopsies in prostate cancer.
abstract: OBJECTIVE
  To evaluate if DNA ploidy analysis with a proliferation index (PI) derived from DNA cytometry of imprints from core needle biopsies predicts disease progression in patients with prostate cancer.
  
  
  METHODS
  Touch imprints were done on a consecutive series of core needle biopsies taken by the same urologist from 240 patients with suspected prostate cancer, 137 (46%) of whom were found to have prostate cancer and included in the study. Scattered cells to the right of the image cytometry (ICM) ploidy-establishing peak, the S-phase fraction, and those in the G2M area of the ICM DNA histograms, were counted in percent of the total number of tumor cells, this value being designated the ICM PI. Based on previous results in archival fine needle aspiration material, the following classification was used: DNA group I, diploid tumor with a low PI; DNA group II, diploid tumor with an intermediate PI and tetraploid tumor with a low or intermediate PI, and DNA group III, diploid or tetraploid tumor with high PI and all tumors with an aneuploid pattern.
  
  
  RESULTS
  Correlation was found to exist between DNA groups I-III and Gleason score (GS) (p < 0.0001), T-stage (p = 0.006), M-stage (p = 0.009) and disease progression (p < 0.0001). Among the 39 patients who had curative treatment and GS 5-7, the progression-free survival rate was 100% in DNA group I, as compared with only 38% in DNA group II and 55% in DNA group III within the follow-up period (p = 0.008).
  
  
  CONCLUSIONS
  DNA ploidy combined with a PI derived from image cytometry of imprints from core needle biopsies yields additional prognostic information in patients with GS 5-7. Diploid tumors with a low PI (DNA group I) are associated with a low risk of disease progression.
authors.affiliation: Department of Urology at the University of Lund, University Hospital, Malmö, Sweden. fam.ahlgren@telia.com
authors.forename: G
authors.initials: G
authors.lastname: Ahlgren
authors.forename: U
authors.initials: U
authors.lastname: Falkmer
authors.forename: V
authors.initials: V
authors.lastname: Gadaleanu
authors.forename: P A
authors.initials: PA
authors.lastname: Abrahamsson
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001707:Biopsy, Needle; D018450:Disease Progression; D018572:Disease-Free Survival; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006801:Humans; D019044:Image Cytometry; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D009367:Neoplasm Staging; D011003:Ploidies; D011237:Predictive Value of Tests; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000473:pathology* / Q000628:therapy; D012680:Sensitivity and Specificity
chemical_list: D017430:Prostate-Specific Antigen