Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera: Geotrupidae) using banding and fluorescent in-situ hybridization.
Mitotic metaphase chromosomes of the scarab beetle Thorectes intermedius (Costa) (Coleoptera Scarabaeoidea: Geotrupidae) were analyzed using various banding methods and fluorescent in-situ hybridization (FISH) with a ribosomal probe. The results obtained indicate that silver and CMA3 staining are unable to localize the chromosome sites of nucleolar organizer regions (NORs). Such an inadequacy is a consequence of the extensive silver and CMA3 stainability of both constitutive heterochromatin and heterochromatin associated to the NORs.
Vitturi
R
R
Dipartimento di Biologia Animale, Università di Palermo, Italy.
Colomba
M S
MS
Barbieri
R
R
Zunino
M
M
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D001517:Coleoptera / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region
D004275:DNA, Ribosomal