Cloning of an alpha-glucosidase gene from Thermococcus hydrothermalis by functional complementation of a Saccharomyces cerevisiae mal11 mutant strain.
alpha-Glucosidase is found in methanogenic and thermophilic archaea and also in eukaryotes and bacteria. The gene encoding the enzyme was cloned from Thermococcus hydrothermalis by complementation of a Saccharomyces cerevisiae deficiency maltase mutant strain. The gDNA clone isolated encodes an open reading frame corresponding to a protein of 242 amino acids. The protein shows 42% identity to a Pyrococcus horikoshii unknown ORF but no similarities were obtained with polysaccharidase sequences.
Galichet
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Laboratoire de Microbiologie Générale et Moléculaire, UFR Sciences, Université de Reims, France.
Belarbi
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004789:Enzyme Activation / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction; D019711:Pyrococcus / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D027981:Symporters; D019712:Thermococcus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic; D000520:alpha-Glucosidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D004269:DNA, Bacterial; D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D000520:alpha-Glucosidases