Repair of apurinic/apyrimidinic sites by UV damage endonuclease; a repair protein for UV and oxidative damage.
UV damage endonuclease (UVDE) initiates a novel form of excision repair by introducing a nick imme-diately 5" to UV-induced cyclobutane pyrimidine dimers or 6-4 photoproducts. Here, we report that apurinic/apyrimidinic (AP) sites are also nicked by Neurospora crassa and Schizosaccharomyces pombe UVDE. UVDE introduces a nick immediately 5" to the AP site leaving a 3"-OH and a 5"-phosphate AP. Apyrimidinic sites are more effectively nicked by UVDE than apurinic sites. UVDE also possesses 3"-repair activities for AP sites nicked by AP lyase and for 3"-phosphoglycolate produced by bleomycin. The Uvde gene introduced into Escherichia coli cells lacking two types of AP endonuclease, Exo III and Endo IV, gave the host cells resistance to methylmethane sulfonate and t-butyl hydroperoxide. We identified two AP endonuclease activities in S.pombe cell extracts. Besides cyclobutane pyrimidine dimers and 6-4 photoproducts, N. crassa UVDE also nicks Dewar photoproducts. Thus, UVDE is able to repair both of the major forms of DNA damage in living organisms: UV-induced DNA lesions and AP sites.
Kanno
S
S
Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.
Iwai
S
S
Takao
M
M
Yasui
A
A
D001080:Apurinic Acid / Q000235:genetics / Q000378:metabolism*; D001761:Bleomycin / Q000494:pharmacology; D019757:Carbon-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics*; D045647:DNA Glycosylases; D004260:DNA Repair / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D006016:Glycolates / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010084:Oxidation-Reduction; D011119:Polynucleotides / Q000235:genetics / Q000378:metabolism*; D011740:Pyrimidine Dimers / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D014466:Ultraviolet Rays; D051981:Uracil-DNA Glycosidase; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; D006016:Glycolates; D009838:Oligodeoxyribonucleotides; D011119:Polynucleotides; D011740:Pyrimidine Dimers; D029702:Schizosaccharomyces pombe Proteins; C001790:apyrimidinic acid; D001080:Apurinic Acid; D001761:Bleomycin; D020122:tert-Butylhydroperoxide; D008741:Methyl Methanesulfonate; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; C105073:uve1 protein, S pombe; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; C013193:phosphoglycolate