Growth of MDA-MB-231 cell line: different effects of TGF-beta(1), EGF and estradiol depending on the length of exposure.
The human cell line MDA-MB-231 is a prototype for the study of hormone-independent breast cancer. Modification of cell growth behaviour has been observed after treating these cells with growth factors. EGF is a typical stimulatory growth factor for many cell types, whereas transforming growth factor beta(1)(TGF-beta(1)) acts with inhibitory character. Here we observed cell growth inhibition after EGF as well as after TGF-beta(1)treatments. Nevertheless, in the 42-h experiments, EGF-treated cultures grew before (18 hours) respect to the TGF-beta(1)and E(2)-treated cultures (24 h), and in the 11-day experiments, EGF-treated cultures started growing (7 days) after TGF-beta(1)-treated cultures (5 days). Estradiol inhibited the proliferation of these cells only after several days of treatment.
Mur
C
C
Hormone Unit, Biochemistry Department, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.
Martínez-Carpio
P A
PA
Fernández-Montolí
M E
ME
Ramon
J M
JM
Rosel
P
P
Navarro
M A
MA
D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000494:pharmacology* / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D006801:Humans; D015398:Signal Transduction; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000502:physiology; D014407:Tumor Cells, Cultured
D016212:Transforming Growth Factor beta; D004958:Estradiol; D004815:Epidermal Growth Factor