title: Activation-induced T-cell death and immune dysfunction after implantation of left-ventricular assist device.
abstract: BACKGROUND
  Cardiac transplantation is a limited option for end-stage heart failure because of the shortage of donor organs. Left-ventricular assist devices (LVADs) are currently under investigation as permanent therapy for end-stage heart failure, but long-term successful device implantation is limited because of a high rate of serious infections. To examine the relation between LVAD-related infection and host immunity, we investigated immune responses in LVAD recipients.
  
  
  METHODS
  We compared the rate of candidal infection in 78 patients with New York Heart Association class IV heart failure who received either an LVAD (n=40) or medical management (controls, n=38). Fluorochrome-labelled monoclonal antibodies were used in analyses of T-cell phenotype. Analysis of T-cell function included intradermal responses to recall antigens and proliferative responses after stimulation by phytohaemagglutinin, monoclonal antibodies to CD3, and mixed lymphocyte culture. We measured T-cell apoptosis in vivo by annexin V binding, and confirmed the result by assessment of DNA fragmentation. Activation-induced T-cell death was measured after T-cell stimulation with antibodies to CD3. All immunological tests were done at least 1 month after LVAD implantation. Between-group comparisons were by Kaplan-Meier actuarial analysis and Student's t test.
  
  
  RESULTS
  By 3 months after implantation of LVAD, the risk of developing candidal infection was 28% in LVAD recipients, compared with 3% in controls (p=0.003). LVAD recipients had cutaneous anergy to recall antigens and lower (<70%) T-cell proliferative responses than controls after activation via the T-cell receptor complex (p<0.001). T cells from LVAD recipients had higher surface expression of CD95 (Fas) (p<0.001) and a higher rate of spontaneous apoptosis (p<0.001) than controls. Moreover, after stimulation with antibodies to CD3, CD4 T-cell death increased by 3.2-fold in LVAD recipients compared with only 1.2-fold in controls (p<0.05).
  
  
  CONCLUSIONS
  LVAD implantation results in an aberrant state of T-cell activation, heightened susceptibility of CD4 T cells to activation-induced cell death, progressive defects in cellular immunity, and increased risk of opportunistic infection.
authors.affiliation: Department of Surgery, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
authors.forename: H J
authors.initials: HJ
authors.lastname: Ankersmit
authors.forename: S
authors.initials: S
authors.lastname: Tugulea
authors.forename: T
authors.initials: T
authors.lastname: Spanier
authors.forename: A D
authors.initials: AD
authors.lastname: Weinberg
authors.forename: J H
authors.initials: JH
authors.lastname: Artrip
authors.forename: E M
authors.initials: EM
authors.lastname: Burke
authors.forename: M
authors.initials: M
authors.lastname: Flannery
authors.forename: D
authors.initials: D
authors.lastname: Mancini
authors.forename: E A
authors.initials: EA
authors.lastname: Rose
authors.forename: N M
authors.initials: NM
authors.lastname: Edwards
authors.forename: M C
authors.initials: MC
authors.lastname: Oz
authors.forename: S
authors.initials: S
authors.lastname: Itescu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D002177:Candidiasis / Q000453:epidemiology / Q000209:etiology*; D016923:Cell Death; D005260:Female; D005434:Flow Cytometry; D006333:Heart Failure / Q000150:complications / Q000276:immunology* / Q000601:surgery*; D006353:Heart-Assist Devices / Q000009:adverse effects* / Q000382:microbiology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*