[Melatonin inhibits TRH-stimulating prolactin gene expression of anterior pituitary cells in newborn rat in vitro].
OBJECTIVE
This work was to investigate whether melatonin (MEL) plays a role in the gene expression of prolactin (PRL), by the Method of in situ hybridyzation.


RESULTS
Our results indicated that, at a higher concentration, MEL not only inhibits TRH (thyrotropin releasing hormone) stimulating PRL gene expression of anterior pituitary cell in newborn rat, but also exerts a direct inhibitory effect on PRL gene expression in vitro.


CONCLUSIONS
These results suggest that MEL may be a regulator of PRL synthesis and secreting in vivo.
Di
A
A
Institute of Basic Medical Sciences, Beijing.
Xu
R
R
Peng
S
S
Shan
H
H
Qian
Z
Z
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D015870:Gene Expression; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D011388:Prolactin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013973:Thyrotropin-Releasing Hormone / Q000037:antagonists & inhibitors*
D012333:RNA, Messenger; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin; D008550:Melatonin