title: Pseudo-continuous arterial spin labeling technique for measuring CBF dynamics with high temporal resolution.
abstract: Cerebral blood flow (CBF) can be measured noninvasively with nuclear magnetic resonance (NMR) by using arterial water as an endogenous perfusion tracer. However, the arterial spin labeling (ASL) techniques suffer from poor temporal resolution due to the need to wait for the exchange of labeled arterial spins with tissue spins to produce contrast. In this work, a new ASL technique is introduced, which allows the measurement of CBF dynamics with high temporal and spatial resolution. This novel method was used in rats to determine the dynamics of CBF changes elicited by somatosensory stimulation with a temporal resolution of 108 ms. The onset time of the CBF response was 0.6 +/- 0.4 sec (mean +/- SD) after onset of stimulation (n = 10). The peak response was observed 4.4 +/- 3.7 sec (mean +/- SD) after stimulation began. These results are in excellent agreement with previous data obtained with invasive techniques, such as laser-Doppler flowmetry and hydrogen clearance, and suggest the appropriateness of this novel technique to probe CBF dynamics in functional and pathological studies with high temporal and spatial resolution. Magn Reson Med 42:425-429, 1999.
authors.affiliation: Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota Medical School, Minneapolis, Minnesota, USA. afonso@cmrr.umn.edu
authors.forename: A C
authors.initials: AC
authors.lastname: Silva
authors.forename: S G
authors.initials: SG
authors.lastname: Kim
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation / Q000502:physiology*; D017352:Echo-Planar Imaging / Q000379:methods*; D004558:Electric Stimulation; D007089:Image Enhancement / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels
chemical_list: D013113:Spin Labels