Sequence-specific alkylation of DNA by duocarmycin A and its novel derivatives bearing PY/IM polyamides.
A new class of sequence-specific DNA alkylating agents were developed based on the reactivity of duocarmycin A and the DNA-reading ability of pyrrole-imidazole polyamide. The DNA alkylation sequence specificity by duocarmycin A can be modulated by a variety of pyrrole-imidazole triamides in a predictable manner. Novel hybrids of the segment A of duocarmycin A and pyrrole-imidazole polyamides efficiently and highly selectively alkylated the target base possessing match sequences of Dervan's binding code.
Tao
Z F
ZF
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
Fujiwara
T
T
Saito
I
I
Sugiyama
H
H
D000477:Alkylating Agents / Q000737:chemistry*; D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D000080890:Duocarmycins; D007211:Indoles; D009757:Nylons / Q000737:chemistry*; D011760:Pyrrolidinones / Q000737:chemistry
D000477:Alkylating Agents; D000080890:Duocarmycins; D007211:Indoles; D009757:Nylons; D011760:Pyrrolidinones; D004247:DNA; C058289:duocarmycin A