Lens formation by pigmented epithelial cell reaggregate from dorsal iris implanted into limb blastema in the adult newt.
In newt lens regeneration, the dorsal iris has lens forming ability and the ventral iris has no such capability, whereas there is no difference in the morphological criteria. To investigate the real aspects of this characteristic lens regeneration in the newt at the cellular level, a useful model system was constructed by transplanting the dorsal and ventral reaggregate derived from singly dissociated pigmented epithelial cells of the iris into the blastema of the forelimb in the newt. The lens was formed from the dorsal reaggregate with high efficiency, but not from the ventral one. No lens formation was observed in the implantation of the reaggregate into the tissue of the intact limbs. In detailed examination of the process of lens formation from the reaggregate, it was shown that tubular formation was the first step in the rearrangement of cells within the reaggregate. This was followed by depigmentation, vesicle formation with active cell growth, and the final step was lens fiber formation by transdifferentiation of epithelial cells composing the lens vesicle. The process was almost the same as in situ lens regeneration except the reconstitution of the two-layered epithelial structure was embodied as flattened tubular formation in the first step. The present study made it possible for the first time to examine lens forming ability in the reaggregate mixed with dorsal and ventral cells, because the formation of a reaggregate was started from singly dissociated cells of the dorsal and ventral cells of the iris. Mixed reaggregate experiments indicated that the existence of the dorsal cells in a cluster within the reaggregate is important in lens formation, and ventral cells showed an inhibitory effect on the formation. The present study demonstrated that the limb system thus constructed was effective for the analysis of lens formation at the cellular level and made it possible to examine the role of dorsal and ventral cells in lens regeneration.
Ito
M
M
Division of Biological Science, Graduate School of Science, Nagoya University, Japan.
Hayashi
T
T
Kuroiwa
A
A
Okamoto
M
M
D000818:Animals; D005260:Female; D005552:Forelimb / Q000502:physiology*; D007150:Immunohistochemistry; D007498:Iris / Q000033:anatomy & histology / Q000502:physiology / Q000637:transplantation*; D007908:Lens, Crystalline / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000033:anatomy & histology / Q000502:physiology / Q000637:transplantation*; D012038:Regeneration; D012455:Salamandridae / Q000033:anatomy & histology / Q000502:physiology*; D016042:Transplantation, Heterotopic