Protein delipidation and precipitation by tri-n-butylphosphate, acetone, and methanol treatment for isoelectric focusing and two-dimensional gel electrophoresis.
Mastro
R
R
Australian Institute of Marine Science, Townsville, 4810, Australia.
Hall
M
M
D000096:Acetone; D000818:Animals; D002021:Buffers; D011232:Chemical Precipitation; D033364:Decapoda; D004527:Egg Proteins / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D007525:Isoelectric Focusing / Q000379:methods*; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol; D010755:Organophosphates; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D012997:Solvents
D002021:Buffers; D004527:Egg Proteins; D008055:Lipids; D010755:Organophosphates; D011506:Proteins; D012997:Solvents; D000096:Acetone; C009524:tributyl phosphate; D000432:Methanol