[Antitumor effect on melanoma by in vivo direct injection of packaging cells transfected recombinant IL-2 gene].
Our previous studies showed that ex vivo transfer of human IL-2 gene into murine B16 melanoma cells resulted in the expression of IL-2 activity and had inhibitory effect on tumor growth in vivo. In this study, B16 melanoma bearing mice were injected directly with IL-2 recombinant PA317 cells. The in vivo gene transfer revealed significant reduction of tumor size and prolongation of survival of tumor bearing mice. Using slot blot analysis, the expression of IL-2 mRNA in tumor mass was detected and the IFN gamma activity was also enhanced in gene transferred mice. These results indicated that in vivo direct transfer of IL-2 recombinant retrovirual vector producing cells would induce transfer gene into tumor cells and exert its antitumor effect.
Zhu
Y
Y
Cancer Institute, CAMS, Beijing.
Wu
Y
Y
Hui
Y
Y
D000818:Animals; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma