Comparison of in vivo 1H MRS of human brain tumours with 1H HR-MAS spectroscopy of intact biopsy samples in vitro.
High resolution magic angle spinning (MAS) 1H nuclear magnetic resonance (NMR) spectroscopy has been employed to study intact human brain tumour tissue and comparison with the corresponding in vivo spectrum has been made. Two dimensional 1H MAS-NMR measurements, including J-resolved and homonuclear shift correlation spectra, were obtained to aid metabolite signal assignment. MAS gave greatly improved line-shape and reduced line-width in comparison to conventional high resolution in vivo 1H MRS of intact tissue, permitting the simultaneous detection of cellular lipids and metabolites. The technique provides the most direct method for comparison of in vivo spectra with high resolution spectra in vitro and hence allows more reliable peak assignment of in vivo 1H MRS spectra.
Barton
S J
SJ
CRC Biomedical Magnetic Resonance Research Group, Division of Biochemistry, St. George's Hospital Medical School, London, UK.
Howe
F A
FA
Tomlins
A M
AM
Cudlip
S A
SA
Nicholson
J K
JK
Bell
B A
BA
Griffiths
J R
JR
D001254:Astrocytoma / Q000737:chemistry*; D001706:Biopsy; D001932:Brain Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D005909:Glioblastoma / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008579:Meningioma / Q000737:chemistry*