Detection of chromosome loss and gain induced by griseofulvin, estramustine, and vanadate in binucleated lymphocytes using FISH analysis.
Cytochalasin B-blocked binucleated human lymphocytes from a healthy male donor were used to detect micronucleus induction and other aneuploidy events (chromosome loss and gain) after treatment with griseofulvin (GF), estramustine (EM), and sodium orthovanadate (Na(3)VO(4)). A two-color FISH was performed by using centromeric probes for chromosome 2 (FITC labeled) and the X chromosome (TRITC labeled) to measure chromosome loss and gain events in binucleated cells. GF induced mainly aneuploid binucleates involving the X chromosome, but this was not associated with preferential loss of one of the two chromosomes. EM preferentially induced aneuploidy of chromosome 2, and Na(3)VO(4) of the X chromosome. Our results indicate that chromosome malsegregation events (chromosome loss and/or gain) are probably not randomly induced, suggesting that different mechanisms leading to aneuploidy may be either chromosome-dependent or compound- and dose- related.
Migliore
L
L
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Università di Pisa, Italy. l.migliore@geog.unipi.it
Zotti-Martelli
L
L
Scarpato
R
R
D000782:Aneuploidy; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D015342:DNA Probes; D004961:Estramustine / Q000494:pharmacology*; D006118:Griseofulvin / Q000494:pharmacology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000235:genetics; D015162:Micronucleus Tests; D014638:Vanadates / Q000494:pharmacology*; D014960:X Chromosome / Q000235:genetics
D015342:DNA Probes; D006118:Griseofulvin; D004961:Estramustine; D014638:Vanadates