Effect of disodium cromoglycate treatment on peripheral blood mononuclear cell adhesion to cultured endothelium in allergic asthmatics.
In this study we have compared the adhesion of peripheral blood mononuclear cells (PBMC) to human umbilical vein endothelial cells (HUVEC) in a healthy control group with two groups of allergic asthmatics, not treated or treated with disodium cromoglycate (DSCG). The adhesion and blocking experiments were performed by the flow cytometric adhesion assay. No differences in the adhesion of lymphocytes were observed in any of the groups. The monocytes obtained from DSCG non-treated patients have shown significant (P < 0.05) enhancement of adhesion to HUVEC in comparison to healthy controls. The treatment of asthmatic patients with DSCG downregulated the monocyte adhesion to cultured endothelial cells (ECs) and this was comparable to the group of normal donors. The DSCG may have a therapeutic effect on the regulation of monocyte adhesion in inflammatory and allergic diseases. The binding ability of untreated asthmatic PBMC to cultured ECs was partially inhibited by monoclonal antibody anti-CD54, suggesting that the increased EC adhesiveness for monocytes from allergic asthmatics may be at least partially dependent on the ICAM-1 adhesion pathways. Our results also indicate that the blocking agent anti-CD18 was not essential for monocyte-endothelial interactions in allergic asthma.
Horváthová
M
M
Institute of Preventive and Clinical Medicine, Department of Clinical Immunology, Bratislava, Slovak Republic.
Podivínsky
F
F
Gazdík
F
F
Jahnová
E
E
D000328:Adult; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001249:Asthma / Q000097:blood* / Q000188:drug therapy*; D018821:CD18 Antigens / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004205:Cromolyn Sodium / Q000627:therapeutic use*; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D009000:Monocytes / Q000276:immunology / Q000502:physiology*; D012016:Reference Values
D018927:Anti-Asthmatic Agents; D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D018799:Intercellular Adhesion Molecule-1; D004205:Cromolyn Sodium