A simple and rapid method for analyzing the Monascus pigment-mediated degradation of mutagenic 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography.
A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4, 3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min.
Watanabe
T
T
Yaegaki Technology Development Laboratories, Yaegaki Bio-industry, 681 Hayashida, Himeji, Japan.
Mazumder
T K
TK
Yamamoto
A
A
Nagai
S
S
Arimoto-Kobayashi
S
S
Hayatsu
H
H
Terabe
S
S
D016587:Antimutagenic Agents / Q000737:chemistry / Q000378:metabolism; D001203:Ascomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002243:Carbolines / Q000032:analysis / Q000378:metabolism*; D002384:Catalysis; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D009153:Mutagens / Q000032:analysis / Q000378:metabolism*; D010860:Pigments, Biological / Q000737:chemistry / Q000378:metabolism*
D016587:Antimutagenic Agents; D002243:Carbolines; D009153:Mutagens; D010860:Pigments, Biological; C027210:3-hydroxyamino-1-methyl-5H-pyrido(4,3-b)indole