title: Growth of MDA-MB-231 cell line: different effects of TGF-beta(1), EGF and estradiol depending on the length of exposure.
abstract: The human cell line MDA-MB-231 is a prototype for the study of hormone-independent breast cancer. Modification of cell growth behaviour has been observed after treating these cells with growth factors. EGF is a typical stimulatory growth factor for many cell types, whereas transforming growth factor beta(1)(TGF-beta(1)) acts with inhibitory character. Here we observed cell growth inhibition after EGF as well as after TGF-beta(1)treatments. Nevertheless, in the 42-h experiments, EGF-treated cultures grew before (18 hours) respect to the TGF-beta(1)and E(2)-treated cultures (24 h), and in the 11-day experiments, EGF-treated cultures started growing (7 days) after TGF-beta(1)-treated cultures (5 days). Estradiol inhibited the proliferation of these cells only after several days of treatment.
authors.affiliation: Hormone Unit, Biochemistry Department, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Mur
authors.forename: P A
authors.initials: PA
authors.lastname: Martínez-Carpio
authors.forename: M E
authors.initials: ME
authors.lastname: Fernández-Montolí
authors.forename: J M
authors.initials: JM
authors.lastname: Ramon
authors.forename: P
authors.initials: P
authors.lastname: Rosel
authors.forename: M A
authors.initials: MA
authors.lastname: Navarro
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000494:pharmacology* / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D006801:Humans; D015398:Signal Transduction; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D016212:Transforming Growth Factor beta; D004958:Estradiol; D004815:Epidermal Growth Factor