title: Expression of the fusion protein recombinant human granulocyte-macrophage colony stimulating factor and leukemia inhibitory factor in a baculovirus vector system.
abstract: A fusion gene coding human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) cDNAs was inserted into the transfer vector pSXIVVI+ X3 with the control of Syn and XIV promoters. The Sf9 cells (Spodoptera frugiperda) were co-transfected with the recombinant plasmid and TnNPV DNA (Trichoplusia ni nuclear polyhedrosis virus DNA). The fusion protein recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) could be synthesized in cells infected with recombinant virus at a level of about 23% of their total cellular protein. Activity analysis of the fusion protein in infected cells revealed that it exhibited the dual activities of GM-CSF and LIF. Western blot analysis of the expressed fusion protein in infected larvae showed that the virus-mediated fusion protein, with a molecular weight of approximately 35 kDa, is confirmed with immunoreactivity.
authors.affiliation: Food Engineering Research Center of State Education Department, Zhongshan University, Guangzhou 510275, People's Republic of China.
authors.initials: XW
authors.lastname: Zhang
authors.initials: XY
authors.lastname: Zeng
authors.initials: T
authors.lastname: Sun
authors.initials: X
authors.lastname: Liu
authors.initials: DX
authors.lastname: Gu