title: Microalbuminuria in diabetic adolescents and children--feasibility phase of a national cross-sectional study. MIDAC Research Group.
abstract: Our objective was to assess the feasibility of examining the prevalence of microalbuminuria nationally and to validate the effectiveness of the collection and central analysis of biological specimens. In a centrally coordinated, cross-sectional, multicenter pilot study in pediatric, diabetes outpatients clinics in UK and the Republic of Ireland, blood and urine samples were collected from patients aged between 10 and 20 years, with a duration of insulin-dependent diabetes greater than 1 year attending diabetes outpatient clinics, between April 1996 and February 1997. These samples were analyzed at a central reference laboratory for HbA1C using high performance liquid chromatography (HPLC) and for urinary albumin and creatinine concentrations using spectrophotometry. Clinical data were collected locally and sent to the coordinator for analysis. A total of 419 patients were recruited to the study from ten centers of whom 308 (74%) correctly provided three timed overnight urine samples; 13 patients (4.2%) were microalbuminuric (AER > or =20 microg min(-1) in at least two of three urine samples), 1 (0.3%) was proteinuric (AER > or = 200 microg min(-1) in at least two of three urine samples). All patients found to have microalbuminuria and proteinuria were pubertal or post-pubertal. This study demonstrates that it is possible to coordinate a network of centers in the United Kingdom and the Republic of Ireland for the purposes of diabetes research in young adults and children. The level of abnormal albumin excretion of 4.5% is less than previously described in such age groups in other countries.
authors.affiliation: Division of Child Health, University of Bristol, Royal Hospital for Sick Children, United Kingdom.
authors.forename: T H
authors.initials: TH
authors.lastname: Moore
authors.forename: J P
authors.initials: JP
authors.lastname: Shield
mesh_terms: D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000453:epidemiology* / Q000652:urine; D001794:Blood Pressure; D015992:Body Mass Index; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000652:urine; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000652:urine*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007494:Ireland; D008297:Male; D010865:Pilot Projects; D011627:Puberty; D013053:Spectrophotometry; D006113:United Kingdom
chemical_list: D003404:Creatinine; D006442:Glycated Hemoglobin