N-terminal truncated cytochrome P450 2B4: catalytic activities and reduction with alternative electron sources.
It was shown that riboflavin binds to the truncated cytochrome P450 2B4 and forms a complex with the K(d) = 26 microM. Noncovalent complex of truncated (Delta2-27) cytochrome P450 2B4 with riboflavin was essential for electron transfer realization and catalyzed the NADH-dependent and hydrogen peroxide-supported monooxygenase reactions of aminopyrine N-demethylation and aniline p-hydroxylation. Flavocytochrome molecular maquette was capable of supporting photoactivatable electron transfer and could be photoreduced and electroreduced quantitatively in the absence of pyridine nucleotides.
Shumyantseva
V V
VV
Institute of Biomedical Chemistry, Pogodinskaya Street 10, Moscow, 119832, Russia. victoria@ibmh.msk.su
Bulko
T V
TV
Alexandrova
S A
SA
Sokolov
N N
NN
Schmid
R D
RD
Bachmann
T
T
Archakov
A I
AI
D000632:Aminopyrine / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D002384:Catalysis; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli; D007700:Kinetics; D010084:Oxidation-Reduction; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012256:Riboflavin / Q000378:metabolism*; D017384:Sequence Deletion; D013250:Steroid Hydroxylases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D000814:Aniline Compounds; D011994:Recombinant Proteins; D000632:Aminopyrine; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C050356:steroid 15-alpha-hydroxylase; C023650:aniline; D012256:Riboflavin