Ethanol inhalation on 1-[14C]-pyruvate kinetics in mice using a six-compartment closed model.
1-[14C]-pyruvate kinetics were studied in mice with and without inhalation of vaporised ethanol. The 1-[14C]-pyruvate kinetics were modelled by a six-compartment closed model, i.e. injected site, blood, periphery, expired 14CO2 in air, eliminated 14C in urine and faeces, using the system of differential equations. The results show that the inhalation of vaporised ethanol can stimulate expiration of 14CO2. The completely analytical solution of the six-compartment closed model was found using Laplace transform. The kinetic parameters were estimated using the analytical solutions for the fourth, fifth and sixth compartments to fit eliminated 14CO2, and 14C in urine and faeces. The compartmental analysis showed that the inhalation of vaporised ethanol can stimulate 1-[14C]-pyruvate transmembrane process from injected site to blood and 14C trans-tissue process from periphery to blood.
Wu
G
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Department of Biochemistry, Faculty of Biology, Odessa State University, Ukraine.
D000280:Administration, Inhalation; D000818:Animals; D002244:Carbon / Q000652:urine; D002245:Carbon Dioxide / Q000032:analysis*; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology*; D005243:Feces / Q000737:chemistry; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D019289:Pyruvic Acid / Q000493:pharmacokinetics*; D013223:Statistics as Topic; D013997:Time Factors
D002245:Carbon Dioxide; D000431:Ethanol; D002244:Carbon; D019289:Pyruvic Acid