title: Secretion of rubella virions and virus-like particles in cultured epithelial cells.
abstract: Rubella virus (RV) is an enveloped RNA virus that causes systemic infections in humans. More importantly, first trimester in utero infection leads to a collection of devastating birth defects known as congenital rubella syndrome. Epithelial cells are the first line of defense against viruses and consequently, the polarity of virus secretion is an important factor affecting viral spread. As a first step toward understanding how RV interacts with epithelial cells, we have examined the release of RV-like particles and virions from polarized cells in culture. RV structural proteins were targeted to the Golgi complex and virus particle formation occurred on intracellular membranes in three different polarized epithelial cells. Polarized cells could be infected from the apical and basal membranes, indicating that receptors are not confined to one surface. The secretion of virus-like particles and infectious virions varied according to cell type. In two of the three polarized cell lines examined, virus was released primarily from the apical surface, but significant quantities were also secreted from the basolateral membrane. Release of virus from the apical surface may facilitate virus spread from person to person, whereas basolateral secretion could be important for establishing a systemic infection and/or crossing the placenta prior to fetal infection.
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, T6G 2H7, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Garbutt
authors.forename: H
authors.initials: H
authors.lastname: Chan
authors.forename: T C
authors.initials: TC
authors.lastname: Hobman
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D012409:Rubella / Q000473:pathology / Q000821:virology*; D012412:Rubella virus / Q000502:physiology*; D014771:Virion / Q000502:physiology*; D019065:Virus Assembly; D014779:Virus Replication