title: HLA characterization in adult asymptomatic cyst passers of Entamoeba histolytica/E. dispar.
abstract: The present work aimed at studying the possible association of HLA antigens with Entamoeba histolytica/E. dispar asymptomatic infection in a Mexican mestizo population. A case-control design was selected for evaluation of the role of genetic markers in parasite infection. For this purpose the HLA-A, HLA-B, and HLA-DR profiles of a population of asymptomatic E. histolytica/E. dispar adult cyst passers (cases) and a corresponding nonparasitized adult group (controls) followed for 12 months were identified. Entamoeba species were identified through zymodeme patterns and/or amplification of species-specific DNA sequences. A healthy, nonparasitized group of individuals was included as a control. Our results show that apparently, no specific HLA marker is associated with the asymptomatic cyst passers' condition. These findings have to be added to previous results in which, in contrast to a demonstrated association between HLA-DR3 and amebic liver abscess in Mexican mestizo adults and infants, no significant association with amebic rectocolitis was found.
authors.affiliation: Hospital de Pediatría Centro Médico Siglo XXI, IMSS, México.
authors.forename: E
authors.initials: E
authors.lastname: Valdez
authors.forename: M
authors.initials: M
authors.lastname: del Carmen Martínez
authors.forename: A
authors.initials: A
authors.lastname: Gómez
authors.forename: R
authors.initials: R
authors.lastname: Cedillo
authors.forename: J
authors.initials: J
authors.lastname: Arellano
authors.forename: M E
authors.initials: ME
authors.lastname: Pérez
authors.forename: F
authors.initials: F
authors.lastname: Ramos
authors.forename: P
authors.initials: P
authors.lastname: Morán
authors.forename: E
authors.initials: E
authors.lastname: González
authors.forename: O
authors.initials: O
authors.lastname: Valenzuela
authors.forename: E I
authors.initials: EI
authors.lastname: Melendro
authors.forename: M
authors.initials: M
authors.lastname: Ramiro
authors.forename: R
authors.initials: R
authors.lastname: Kretschmer
authors.forename: O
authors.initials: O
authors.lastname: Muñoz
authors.forename: C
authors.initials: C
authors.lastname: Ximénez
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D016022:Case-Control Studies; D016054:DNA, Protozoan / Q000032:analysis; D004198:Disease Susceptibility; D004404:Dysentery, Amebic / Q000097:blood / Q000469:parasitology*; D004748:Entamoeba histolytica / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D005243:Feces / Q000469:parasitology*; D005500:Follow-Up Studies; D015234:HLA-A Antigens / Q000032:analysis*; D015235:HLA-B Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D016133:Polymerase Chain Reaction
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens