The C-terminal region of the P3 structural protein of rice dwarf phytoreovirus is important for P3-P3 interaction.
Using a random peptide library designed for the yeast two-hybrid system, we identified a peptide that binds strongly to the P3 structural protein of rice dwarf phytoreovirus (RDV). The amino acid sequence of the peptide showed a high homology to the C-terminal region of P3. C-terminally truncated P3 lost its ability to interact with authentic P3. Our observations suggest that the C-terminal region of P3 is important for the P3-P3 interaction, which forms the core shell structure of RDV.
Uyeda
S
S
Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Masuta
C
C
Uyeda
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D012087:Reoviridae / Q000737:chemistry*; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics
D015678:Viral Structural Proteins