Capillary electrochromatography with thermo-optical absorbance detection for the analysis of phenylthiohydantoin-amino acids.
Capillary columns were packed with 3 microns C18 stationary phase, interfaced with an ultraviolet-laser based thermo-optical absorbance detector, and evaluated for separation of a mixture of phenylthiohydantoin-amino acids. These columns demonstrated consistent performance with a relative standard deviation (RSD) for migration time of less than 1.5% and a separation efficiency of 216,000 plates/m for the electroosmotic flow marker, thiourea. The thermo-optical absorbance detector was based on a 248 nm krypton-fluoride excimer laser. Detection limits (3 sigma) ranged from 1.6 to 4.8 x 10(-7) M phenylthiohydantoin (PTH)-amino acid injected onto the column, which is a factor of three superior to those obtained in micellar electrokinetic chromatographic analysis of these compounds. A mixture of 17 PTH amino acids was injected onto the capillary; 13 components were nearly baseline resolved in 14 min.
Qi
M
M
Department of Chemistry, University of Alberta, Edmonton, Canada.
Li
X F
XF
Stathakis
C
C
Dovichi
N J
NJ
D000097:Acetonitriles / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D010669:Phenylthiohydantoin / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results
D000097:Acetonitriles; D000596:Amino Acids; D002021:Buffers; D010669:Phenylthiohydantoin; C032159:acetonitrile