Cloning and sequencing of the bottle-nosed dolphin (Tursiops truncatus) interferon-gamma gene.
The bottle-nosed dolphin (Tursiops truncatus) interferon-gamma(IFN-gamma) cDNA was cloned from mitogen stimulated peripheral blood mononuclear cells (PBMC) RNA utilizing the reverse transcription-polymerase chain reaction (RT-PCR). The dolphin IFN-gammacDNA was 585 base pairs in length and contained an open reading frame of 498 nucleotides encoding an IFN-gammaprecursor of 166 amino acids, with cleavage of the putative signal peptide of 19 amino acids. Analysis of the mature amino acid sequences revealed two potential N-linked glycosylation sites. Comparison of the predicted amino acid sequence showed that dolphin IFN-(gamma)shares 86, 85, 63 and 42% similarity with the bovine, ovine, human and mouse IFN-gamma, respectively.
Inoue
Y
Y
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
Itou
T
T
Oike
T
T
Sakai
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004289:Dolphins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data
D007371:Interferon-gamma