Quantitative analysis of the lobular distribution of S-phase in rat liver following dietary administration of di(2-ethylhexyl)phthalate.
A simple image-analysis method is described, whereby the distribution of hepatocytes that have entered S-phase, as distinguished by the incorporation of bromodeoxyuridine, can be related to the position of the central and portal veins of the hepatic lobule. Hepatocyte S-phase was induced in the livers of male and female F344 rats by administration of di(2-ethylhexyl)phthalate (DEHP) in the diet for 7 days at 2 dose levels, and these livers were used to develop the procedure. The distributions of the S-phase between control and DEHP-treated livers were compared using statistical techniques. The results showed notable differences in the distribution of S-phase between male and female rats as well as limited dose-related effects.
Soames
A R
AR
Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, United Kingdom. Tony.Soames@ctl.zeneca.com
Cliffe
S
S
Pate
I
I
Foster
J R
JR
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001973:Bromodeoxyuridine / Q000378:metabolism; D003627:Data Interpretation, Statistical; D004032:Diet; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D002149:Energy Intake / Q000187:drug effects; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D016196:S Phase; D012737:Sex Factors
D004051:Diethylhexyl Phthalate; D001973:Bromodeoxyuridine