Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen.
The second major allergen of Chamaecyparis obtusa (Japanese cypress) pollen, Cha o 2, has been purified and its cDNA cloned. Of patients with pollinosis caused by C. obtusa, 82.5% produce IgE antibodies which react with purified Cha o 2. The purified protein has a molecular mass of 46 kDa and its 12 N-terminal amino acid sequence displays a high homology with that of Cry j 2, the second major allergen of Cryptomeria japonica pollen. cDNA clones coding for Cha o 2 have been isolated using Cry j 2 cDNA as a probe. Cha o 2 cDNA clones were sequenced and found to code a putative 50-residue signal sequence and a 464-residue mature protein with a molecular weight of 50 kDa. Two possible N-linked glycosylation sites were found in the sequence. The deduced amino acid sequence of Cha o 2 shows 74.3% identity with that of Cry j 2. In its primary structure, Cha o 2 shows significant identity with those of the polygalacturonases of avocado, tomato, and maize as well as Cry j 2.
Mori
T
T
Meiji Institute of Health Science, 540 Naruda, Odawara, Kanagawa, 250-0862, Japan.
Yokoyama
M
M
Komiyama
N
N
Okano
M
M
Kino
K
K
D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001483:Base Sequence; D016022:Case-Control Studies; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology; D011058:Pollen / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014197:Trees
D000485:Allergens; C065291:Cry j II protein, Cryptomeria japonica; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D007073:Immunoglobulin E