title: IUPAC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder.
abstract: This paper presents results of a collaborative trial study (IUPAC project No. 650/93/97) involving 29 laboratories in 13 countries applying a method for detecting genetically modified organisms (GMOs) in food. The method is based on using the polymerase chain reaction to determine the 35S promotor and the NOS terminator for detection of GMOs. reference materials were produced that were derived from genetically modified soy beans and maize. Correct identification of samples containing 2% GMOs is achievable for both soy beans and maize. For samples containing 0.5% genetically modified soy beans, analysis of the 35S promotor resulted also in a 100% correct classification. However, 3 false-negative results (out of 105 samples analyzed) were reported for analysis of the NOS terminator, which is due to the lower sensitivity of this method. Because of the bigger genomic DNA of maize, the probability of encountering false-negative results for samples containing 0.5% GMOs is greater for maize than for soy beans. For blank samples (0% GMO), only 2 false-positive results for soy beans and one for maize were reported. These results appeared as very weak signals and were most probably due to contamination of laboratory equipment.
authors.affiliation: Institute for Health and consumer protection, Food Products unit, Ispra(Va), Italy.
authors.forename: M
authors.initials: M
authors.lastname: Lipp
authors.forename: P
authors.initials: P
authors.lastname: Brodmann
authors.forename: K
authors.initials: K
authors.lastname: Pietsch
authors.forename: J
authors.initials: J
authors.lastname: Pauwels
authors.forename: E
authors.initials: E
authors.lastname: Anklam
authors.forename: T
authors.initials: T
authors.lastname: Börchers
authors.forename: G
authors.initials: G
authors.lastname: Braunschweiger
authors.forename: U
authors.initials: U
authors.lastname: Busch
authors.forename: E
authors.initials: E
authors.lastname: Eklund
authors.forename: F D
authors.initials: FD
authors.lastname: Eriksen
authors.forename: J
authors.initials: J
authors.lastname: Fagan
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authors.initials: A
authors.lastname: Fellinger
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authors.lastname: Gaugitsch
authors.forename: D
authors.initials: D
authors.lastname: Hayes
authors.forename: C
authors.initials: C
authors.lastname: Hertel
authors.forename: H
authors.initials: H
authors.lastname: Hörtner
authors.forename: P
authors.initials: P
authors.lastname: Joudrier
authors.forename: L
authors.initials: L
authors.lastname: Kruse
authors.forename: R
authors.initials: R
authors.lastname: Meyer
authors.forename: M
authors.initials: M
authors.lastname: Miraglia
authors.forename: W
authors.initials: W
authors.lastname: Müller
authors.forename: P
authors.initials: P
authors.lastname: Phillipp
authors.forename: B
authors.initials: B
authors.lastname: Pöpping
authors.forename: R
authors.initials: R
authors.lastname: Rentsch
authors.forename: A
authors.initials: A
authors.lastname: Wurtz
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D017796:Caulimovirus / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005188:False Negative Reactions; D005189:False Positive Reactions; D005504:Food Analysis; D005818:Genetic Engineering; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013025:Glycine max / Q000235:genetics*; D013728:Terminator Regions, Genetic; D003313:Zea mays / Q000235:genetics*