[The transactivation of hepatitis B virus X protein on the expression of Fas ligand in HepG2 cells].
OBJECTIVE
To study the effect of hepatitis B virus x (HBx) protein on the expression of Fas Ligand in HepG2 cells.


METHODS
The open reading frame of HBx gene was obtained from HBV genome (subtype adw) digested with Nco I and Bgl II, then cloned into pCEP4. The reconstructive plasmid pCEP4x was transfected into HepG2 cells by electroportion method. After selecting with hygromycin B, resistant clonies were obtained. The expressions of HBx gene and Fas Ligand gene were detected by RT-PCR. Immunohistochemical staining and western blot analysis.


RESULTS
It showed that Fas Ligand gene expressed in the modified HepG2 cells transfected with plasmid pCEP4x.


CONCLUSIONS
The results suggest the expression of Fas Ligand in HepG2 cells could be activated by HBx protein.
Zhang
D
D
Institute of Viral Hepatitis, Chongqing Medical University.
Zhang
D
D
Tao
X
X
D002460:Cell Line; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein