Functionalized hollow fiber membrane cartridge for adsorption of Anticofactor/Antiphospholipid antibodies: a potential tool for treatment.
It is of considerable interest to ascertain whether a hollow fiber cartridge containing histidine immobilized on polyethylenevinyl alcohol membrane (His-PEVA) is able to retain specific autoantibodies involved in antiphospholipid syndrome. To this end diluted patient pathogenic plasma containing high levels of anti-beta2-glycoprotein I (anti-beta2GPI) and antiprothrombin antibodies was processed through the functionalized cartridge. The adsorbed material was then eluted under mild conditions and analyzed; an enrichment of the eluted fractions in total IgG and more specifically in IgG2 subclass was observed, compared with the injected sample. Enzyme-linked immunosorbent assay tests showed a higher specific binding of antiprothrombin and anti-beta2GPI in these fractions. This was in accordance with the concomitant higher anticoagulant activity measured on the same fractions. All in vitro results clearly demonstrated the ability of the His-PEVA cartridge to preferentially adsorb these autoantibodies. Hence the functionalized cartridge represents a potential tool for the treatment of antiphospholipid syndrome by selective extracorporeal removal of IgG.
Darnige
L
L
Centre Hospitalier, Compiègne, France.
Legallais
C
C
Arvieux
J
J
Pitiot
O
O
Vijayalakshmi
M A
MA
D000327:Adsorption; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000628:therapy; D001053:Apolipoproteins / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology*; D006632:Histamine / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016682:Lupus Coagulation Inhibitor / Q000097:blood; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000628:therapy; D008562:Membrane Glycoproteins / Q000276:immunology*; D008567:Membranes, Artificial; D010956:Plasmapheresis; D020959:Polyethylene / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011516:Prothrombin / Q000276:immunology*; D012709:Serum Albumin / Q000032:analysis; D053482:beta 2-Glycoprotein I
D017152:Antibodies, Antiphospholipid; D001053:Apolipoproteins; D001323:Autoantibodies; D006023:Glycoproteins; D007074:Immunoglobulin G; D016682:Lupus Coagulation Inhibitor; D008562:Membrane Glycoproteins; D008567:Membranes, Artificial; D012709:Serum Albumin; D053482:beta 2-Glycoprotein I; D006632:Histamine; D011516:Prothrombin; D020959:Polyethylene; D011142:Polyvinyl Alcohol