Structural injury of osteosarcoma mitochondria by a novel antitumour agent, 2-methylfuranonaphthoquinone.
The effect of the novel anticancer 2-methylnaphtho[2,3-b]furan-4,9-dione (FNQ3) on human osteosarcoma cell lines (HuO9 and HuO9N2) was investigated. The IC50 values of FNQ3 were 5.95 microM for HuO9 and 3.86 microM for HuO9N2, while that for normal fibroblasts (WI-38 cell line) was 35.8 microM. The selectivity in antitumour activity which was estimated from the IC50 ratio of normal fibroblasts to tumour cells was 6.0 and 9.3 fold for HuO9 and HuO9N2, respectively. FNQ3 at 23.6 microM selectively injured mitochondria of HuO9 cells starting at 36 h and HuO9N2 cells at 24 h, whereas WI-38 cells were unaffected even after 72 h. These results demonstrated that FNQ3 was selectively toxic to the mitochondria of osteosarcoma cells similar to carcinoma cells (Pan et al. (1997) J. Electron Microsc. 46: 181), in comparison to normal cells.
Pan
J
J
Department of Anatomy, Kanazawa Medical University, Ishikawa, Japan.
Koyama
J
J
Matayoshi
A
A
Hirai
K
K
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000648:ultrastructure*; D009285:Naphthoquinones / Q000494:pharmacology*; D012516:Osteosarcoma / Q000188:drug therapy* / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
C106805:2-methylnaphtho(2,3-b)furan-4,9-dione; D000972:Antineoplastic Agents, Phytogenic; D009285:Naphthoquinones