Molecular identification of a murine ubiquitin/60S ribosomal fusion protein and expression study in mouse kidney.
We have isolated and sequenced a 500-bp cDNA clone which is the murine homologue of a ubiquitin/60S ribosomal fusion protein. The cDNA and the deduced amino acid sequence are highly conserved. Northern blotting demonstrates constitutive expression in a mouse renal epithelial cell line and in mouse kidneys. Upon stimulation with interferon-gamma or phorbol esters, the expression is not changed, contrasting with the stimulation seen for polyubiquitin. Expression of the 16-kD ubiquitin/60S protein is also demonstrated in mouse epithelial cells.
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Physiological Institute, University Zürich, Switzerland.
Wüthrich
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RP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D015151:Immunoblotting; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*; D014452:Ubiquitins / Q000235:genetics*
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014452:Ubiquitins; C068468:RPL38 protein, human