Dexamethasone stabilizes IGFBP-1 mRNA in primary cultures of rat hepatocytes.
We have shown that dexamethasone stimulates insulin-like growth factor binding protein-1 (IGFBP-1) production due to an increase in IGFBP-1 mRNA content in primary cultures of rat hepatocytes. In the present study, we investigated the effect of dexamethasone on the stabilization of IGFBP-1 mRNA in this system. We found that dexamethasone stabilized IGFBP-1 mRNA from the dot blot analysis with actinomycin D in the culture medium. It is suggested that the stabilization of IGFBP-1 mRNA is one of the mechanisms in which IGFBP-1 mRNA increases during dexamethasone treatment in primary cultures of rat hepatocytes.
Uchijima
Y
Y
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Takenaka
A
A
Takahashi
S
S
Noguchi
T
T
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003907:Dexamethasone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D017077:Culture Media, Conditioned; D005938:Glucocorticoids; D018970:Insulin-Like Growth Factor Binding Protein 1; D012333:RNA, Messenger; D003907:Dexamethasone