A novel putative low-affinity insulin-like growth factor-binding protein, LIBC (lost in inflammatory breast cancer), and RhoC GTPase correlate with the inflammatory breast cancer phenotype.
Inflammatory breast cancer is a rapidly growing, distinct form of locally advanced breast cancer that carries a guarded prognosis. To identify the genes that contribute to this aggressive phenotype, we compared under- and overexpressed sequences in an inflammatory breast tumor cell line with those of actively replicating normal human mammary epithelial cell lines using differential display. Of the 17 transcripts isolated and characterized from these experiments, overexpression of RhoC GTPase and loss of expression of a novel gene on 6q22, LIBC (lost in inflammatory breast cancer), were highly correlated (P<0.0095 and P<0.0013, respectively) with the inflammatory phenotype when a panel of archival inflammatory breast cancers was compared with noninflammatory stage III breast cancers by in situ hybridization. This study suggests two new molecular markers specific for inflammatory breast cancer.
van Golen
K L
KL
Department of Internal Medicine, The University of Michigan Health System, Ann Arbor 48109, USA.
Davies
S
S
Wu
Z F
ZF
Wang
Y
Y
Bucana
C D
CD
Root
H
H
Chandrasekharappa
S
S
Strawderman
M
M
Ethier
S P
SP
Merajver
S D
SD
D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000302:isolation & purification; D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D055511:CCN Intercellular Signaling Proteins; D002417:Cattle; D055513:Connective Tissue Growth Factor; D004847:Epithelial Cells / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006133:Growth Substances / Q000737:chemistry; D006801:Humans; D017874:Immediate-Early Proteins; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D015203:Reproducibility of Results; D013552:Swine; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D018631:ras Proteins; D020741:rho GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D000075024:rhoC GTP-Binding Protein
D014408:Biomarkers, Tumor; D055511:CCN Intercellular Signaling Proteins; C526317:CCN2 protein, human; C526318:CCN2 protein, mouse; C400081:CCN6 protein, human; D006133:Growth Substances; D017874:Immediate-Early Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor; D020558:GTP Phosphohydrolases; C066690:RHOC protein, human; C512911:Rhoc protein, mouse; D018631:ras Proteins; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein