title: Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line.
abstract: The MHC class I-related Fc receptor, FcRn, mediates the intestinal absorption of maternal IgG in neonatal rodents and the transplacental transport of maternal IgG in humans by receptor-mediated transcytosis. In mice and rats, expression of FcRn in intestinal epithelial cells is limited to the suckling period. We have recently observed, however, clear expression of FcRn in the adult human intestine, suggesting a function for FcRn in intestinal IgG transport beyond neonatal life in humans. We tested this hypothesis using the polarized human intestinal T84 cell line as a model epithelium. Immunocytochemical data show that FcRn is present in T84 cells in a punctate apical pattern similar to that found in human small intestinal enterocytes. Solute flux studies show that FcRn transports IgG across T84 monolayers by receptor-mediated transcytosis. Transport is bidirectional, specific for FcRn, and dependent upon endosomal acidification. These data define a novel bidirectional mechanism of IgG transport across epithelial barriers that predicts an important effect of FcRn on IgG function in immune surveillance and host defense at mucosal surfaces.
authors.affiliation: The Combined Program in Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: B L
authors.initials: BL
authors.lastname: Dickinson
authors.forename: K
authors.initials: K
authors.lastname: Badizadegan
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: J C
authors.initials: JC
authors.lastname: Ahouse
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: N E
authors.initials: NE
authors.lastname: Simister
authors.forename: R S
authors.initials: RS
authors.lastname: Blumberg
authors.forename: W I
authors.initials: WI
authors.lastname: Lencer
mesh_terms: D000328:Adult; D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D016764:Cell Polarity; D006224:Cricetinae; D005260:Female; D015395:Histocompatibility Antigens Class I; D006801:Humans; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000276:immunology / Q000502:physiology*; D008431:Maternal-Fetal Exchange; D051379:Mice; D011247:Pregnancy; D051381:Rats; D011961:Receptors, Fc / Q000276:immunology*; D020298:U937 Cells
chemical_list: D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; D011961:Receptors, Fc; C100688:Fc receptor, neonatal