Selective recognition of a C-G base-pair in the parallel DNA triple-helical binding motif.
Selective recognition of a C-G base-pair within the parallel DNA triple-helical binding motif was achieved by a third strand containing the base 5-methyl pyrimidin-2-one. The third strand affinities (K(D)) for a representative 15-mer duplex sequence containing all four Watson-Crick base pairs (X-Y) in the center are C-G (26 nM) >> A-T (270 nM) approximately T-A (350 nM) > G-C (ca 700 nM).
Prévot-Halter
I
I
Department of Chemistry and Biochemistry, University of Bern, Switzerland.
Leumann
C J
CJ
D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*
C051802:triplex DNA; D004247:DNA