title: The effect of perineural colchicine on nerve injury-induced spinal glial activation and neuropathic pain behavior.
abstract: Factors transported centrally from the site of a peripheral nerve injury are known to provide cellular activation signals to the dorsal root ganglion and spinal cord. Yamamoto and Yaksh [35] were able to use colchicine disruption of axonal transport to abolish thermal hyperalgesia after sciatic chronic constriction in the rat. The current study set out to ascertain whether this observation could be reproduced by applying the same pharmacologic paradigm to a complete, segmentally specific, spinal nerve tight ligation (SPTL) and assessing the impact of this treatment on mechanical allodynia and central, spinal glial activation. Mechanical allodynia of the ipsilateral (lesion side) hind paw was measured at 1, 3, 5, 7, 10, and 14 days following SPTL. Spinal astrocytic and microglial activation were assessed immunohistochemically at 5 and 14 days. Colchicine was unable to prevent mechanical allodynia or spinal glial activation when applied perineurally just proximal to the site of SPTL. Administered alone, colchicine (without SPTL) induced both astrocytic and microglial activation, but not mechanical allodynia. Colchicine applied distal to the site of SPTL did not alter mechanical allodynia or glial responses to SPTL. Neuronal tracing experiments were performed to verify segmental disruption of axonal transport by either SPTL or colchicine treatment. Neuronal tracer injected into the sciatic nerve could not be found at the L5 spinal level following perineural colchicine treatment or tight ligation of the L5 spinal nerve, however, tracer was present at the unobstructed L4 spinal level. These results suggest that central astrocytic and microglial responses may be triggered by disruption of transported signals from the periphery, because they are induced by either colchicine or tight ligation. Conversely, axonally transported factors, either from the site of nerve injury or from the periphery, do not appear to be critical for the development of mechanical allodynia.
authors.affiliation: Department of Anesthesiology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. razor@medschl.cam.ac.uk
authors.forename: R W
authors.initials: RW
authors.lastname: Colburn
authors.forename: J A
authors.initials: JA
authors.lastname: DeLeo
mesh_terms: D000818:Animals; D001370:Axonal Transport; D003078:Colchicine / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000502:physiology / Q000503:physiopathology*; D008297:Male; D009443:Neuritis / Q000503:physiopathology; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology* / Q000503:physiopathology; D012585:Sciatica / Q000503:physiopathology*; D013116:Spinal Cord / Q000502:physiology / Q000503:physiopathology*; D013127:Spinal Nerves / Q000293:injuries* / Q000502:physiology* / Q000503:physiopathology; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
chemical_list: D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D003078:Colchicine