title: [A new scheme for the isolation and purification of human alpha-fetoprotein].
abstract: A new efficient scheme for the isolation and purification of human alpha-fetoprotein from human cord serum was proposed. Sequential chromatography on four columns (Cibacron Blue Sepharose, two metal chelate, and one reversed-phase) helped rapidly prepare alpha-fetoprotein samples of high purity (no less than 98%; purification factor approximately 10(3)) in high yields (approximately 85%).
authors.affiliation: Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russia. tom@ibpm.serpukhov.su
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Tomashevskiĭ
authors.forename: V N
authors.initials: VN
authors.lastname: Uverskiĭ
mesh_terms: D002845:Chromatography / Q000379:methods*; D006801:Humans; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
chemical_list: D000509:alpha-Fetoproteins