Bovine CD4+ T-cells lines reactive with soluble and membrane antigens of Cowdria ruminantium.
Cowdria-specific CD4+ T-cell lines generated from immunised cattle respond to both soluble and membrane proteins of the agent. Furthermore, the lines produced the Cowdria-inhibitory cytokine IFN-gamma in response to soluble antigens fractionated by gel filtration and FPLC. Activity eluted as a single peak around fraction 15 for all T-cell lines tested. This fraction induced the highest production of IFN-gamma by the lines and was shown by SDS-polyacrylamide gel electrophoresis and silver staining analysis to contain less than 10 different bands ranging from 22 to 32 kDa. Given their high sensitivity and specificity, these short-term CD4+ T-cell lines will be valuable tools for the identification of Cowdria antigens for incorporation in a subunit vaccine.
Totté
P
P
CIRAD-EMVT, Pointe-à-Pitre, Guadeloupe, France. totte@cirad.fr
Nyanjui
J
J
Bensaid
A
A
McKeever
D
D
D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology*; D001428:Bacterial Vaccines / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D016995:Ehrlichia ruminantium / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006357:Heartwater Disease / Q000276:immunology / Q000382:microbiology; D007371:Interferon-gamma / Q000096:biosynthesis; D012995:Solubility
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007371:Interferon-gamma