title: The complete genome sequence of PM2, the first lipid-containing bacterial virus To Be isolated.
abstract: Bacteriophage PM2 was isolated from the Pacific Ocean off the coast of Chile in the late 1960s. It was a new virus type, later classified as Corticoviridae, and also the first bacterial virus for which it was demonstrated that lipids are part of the virion structure. Here we report the determination and analysis of the 10, 079-bp circular dsDNA genome sequence. Noteworthy discoveries are the replication initiation system, which is related to the rolling circle mechanism described for phages such as &phi;X174 and P2, and a 1.2-kb sequence that is similar to the maintenance region of a plasmid found in a marine Pseudoalteromonas sp. strain A28.
authors.affiliation: Department of Biosciences and Institute of Biotechnology, Viikki Biocenter, Helsinki, FIN-00014, Finland.
authors.forename: R H
authors.initials: RH
authors.lastname: Männistö
authors.forename: H M
authors.initials: HM
authors.lastname: Kivelä
authors.forename: L
authors.initials: L
authors.lastname: Paulin
authors.forename: D H
authors.initials: DH
authors.lastname: Bamford
authors.forename: J K
authors.initials: JK
authors.lastname: Bamford
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019240:Corticoviridae / Q000737:chemistry* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins; D005814:Genes, Viral / Q000235:genetics / Q000502:physiology; D016679:Genome, Viral; D006090:Gram-Negative Bacteria / Q000821:virology; D008055:Lipids / Q000032:analysis*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D008055:Lipids; D015534:Trans-Activators; D015678:Viral Structural Proteins; C045264:replication initiator protein; D004265:DNA Helicases