Liquid chromatography-fast atom bombardment mass spectrometry for detection and determination of pentazocine in human tissues.
A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 microl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation.
Imamura
T
T
Department of Legal Medicine, Hiroshima University School of Medicine, Japan. tohrui@ipc.hiroshima-u.ac.jp
Kudo
K
K
Namera
A
A
Tokunaga
H
H
Yashiki
M
M
Jitsufuchi
N
N
Kojima
T
T
D000368:Aged; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D010423:Pentazocine / Q000032:analysis*; D015203:Reproducibility of Results; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
D010423:Pentazocine