A light-inducible Myb-like gene that is specifically expressed in red Perilla frutescens and presumably acts as a determining factor of the anthocyanin forma.
The Myb-p1 gene was isolated by screening for differentially expressed Myb-related genes in red (anthocyanin-producing) and green (anthocyanin nonproducing) forms of Perilla frutescens. Expression of Myb-p1 is increased 10-fold in the red relative to the green form of P. futescens, and the gene is induced by light. MYB-P1 has only one DNA-binding region, which corresponds to repeat III in the general structure of MYB proteins. In the yeast two-hybrid system, it was shown that MYB-P1 interacted with MYC-RP, a MYC-related transcriptional regulatory protein involved in the control of anthocyanin biosynthesis in P. frutescens. In yeast, MYB-P1 was able to bind to a dihydroflavonol reductase (DFR) gene promoter isolated from red P. frutescens. These data suggest that Myb-p1 may be involved in the regulation of anthocyanin biosynthesis and could therefore be responsible for determining anthocyanin formation in red P. frutescens.
Gong
Z Z
ZZ
Faculty of Pharmaceutical Sciences, Research Center of Medicinal Resources, Chiba University, Japan.
Yamazaki
M
M
Saito
K
K
D000429:Alcohol Oxidoreductases / Q000235:genetics; D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000096:biosynthesis*; D001483:Base Sequence; D003116:Color; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D016259:Genes, myc; D019686:Lamiaceae / Q000528:radiation effects*; D008027:Light; D008969:Molecular Sequence Data; D009857:Oncogenes; D010940:Plant Proteins / Q000096:biosynthesis*; D010946:Plants, Medicinal / Q000528:radiation effects*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020598:Proto-Oncogene Proteins c-myb; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism
D000872:Anthocyanins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; D014157:Transcription Factors; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase