[Abnormal expression of several oncogenes during hepatocarcinogenesis in WHV/myc transgenic mice].
OBJECTIVE
To explore at cellular level the abnormal expression of related oncogenes during hepatocarcinogenesis in the WHV/myc transgenic mice.


METHODS
With specific molecular probes, in situ hybridization was used to detect the expression of c-myc transgene,IGF-II gene as well as c-jun, c-fos, and c-H-ras oncogenes in the liver sections from WHV/myc transgenic mice in different stages of the carcinogenic process.


RESULTS
The overexpression of c-myc transgene and IGF-II gene transcripts were detected in liver sections from the earlier neonatal transgenic mice. Then, the expression levels of these two genes were rapidly decreased and undetectable. At the time of the liver tumor development, the expression of c-myc transgene and IGF-II gene resumed. The c-jun, c-fos and c-H-ras genes expressed more intensely in the livers from preneoplastic WHV/myc mice than from normal mice.


CONCLUSIONS
The overexpressions of c-myc transgene and IGF-II gene in the earlier neonatal stage and tumoral stage may be of importance for the development and maintenance of a transformed phenotype. During the prenoplastic "silent" period of c-myc transgene expression in WHV/myc transgenic mice, several oncogenes are overexpressed in the livers.
Liu
P
P
First Affiliated Hospital of Nanjing Medical University.
Zhao
Z
Z
Dominique
B
B
D000818:Animals; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009857:Oncogenes
D007335:Insulin-Like Growth Factor II