Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae.
3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase (E.C. 4.1.2.15) catalyses the first step in the biosynthesis of aromatic amino acids: the condensation of phophoenolpyruvate and erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate-7-phosphate. Diffraction-quality crystals of the tyrosine-inhibitable form of the enzyme from Saccharomyces cerevisiae have been obtained by the hanging-drop vapour-diffusion method in the presence of polyethylene glycol. The crystals belong to the triclinic space group P1, with unit-cell parameters a = 81.5, b = 94.0, c = 104.6 A, alpha = 65.5, beta = 85.2, gamma = 75.0 degrees, and can be flash-cooled using glycerol as a cryoprotectant. A data set to 2.3 A has been collected at 120 K.
Schneider
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TR
Lehrstuhl für Strukturchemie, Universtität Göttingen, Tammannstrasse 4, D-37077 Göttingen, Germany. trs@shelx.uni-ac.gwdg.de
Hartmann
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M
Braus
G H
GH
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014443:Tyrosine / Q000378:metabolism / Q000494:pharmacology; D014961:X-Ray Diffraction
D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D014443:Tyrosine; D010723:3-Deoxy-7-Phosphoheptulonate Synthase