title: The role of the rhombic lip in avian cerebellum development.
abstract: We have used a combination of quail-chick fate-mapping techniques and dye labelling to investigate the development of the avian cerebellum. Using Hoxa2 as a guide for the microsurgical construction of quail-chick chimaeras, we show that the caudal boundary of the presumptive cerebellum at E6 maps to the caudal boundary of rhombomere 1. By fate mapping the dorsoventral axis of rhombomere 1, we demonstrate that granule cell precursors are generated at the rhombic lip together with neurons of the lateral pontine nucleus. DiI-labelling of cerebellum explants reveals that external germinal layer precursors have a characteristic unipolar morphology and undergo an orientated, active migration away from the rhombic lip, which is apparently independent of either glial or axon guidance or 'chain' formation.
authors.affiliation: Laboratory of Developmental Neurobiology, Rockefeller University, New York, NY 10021-10034, USA. richard.wingate@kcl.ac.uk
authors.forename: R J
authors.initials: RJ
authors.lastname: Wingate
authors.forename: M E
authors.initials: ME
authors.lastname: Hatten
mesh_terms: D000818:Animals; D002455:Cell Division; D002465:Cell Movement; D002531:Cerebellum / Q000166:cytology / Q000196:embryology*; D002642:Chick Embryo; D002678:Chimera; D003370:Coturnix; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D013234:Stem Cells / Q000166:cytology