title: Crystallization, preliminary X-ray diffraction analysis and Patterson search of oxyhaemoglobin I from the wolf (Chrysocyon brachiurus).
abstract: Oxyhaemoglobin I isolated from the Brazilian wolf Chrysocyon brachiurus has been crystallized and X-ray diffraction data has been collected to 2.06 A resolution using a synchrotron-radiation source. Crystals were determined to belong to the space group P2(1)2(1)2(1) and preliminary structural analysis revealed the presence of one tetramer in the asymmetric unit. The structure was determined using standard molecular-replacement techniques and is currently being refined using maximum-likelihood protocols. This is the first haemoglobin isolated from a member of the Canidae family to be crystallized and it will provide further insights in the comparative biochemistry of vertebrate haemoglobins.
authors.affiliation: Departamento de Física-IBILCE-UNESP, CP 136, CEP 15054-000, São José do Rio Preto, SP, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Smarra
authors.forename: V
authors.initials: V
authors.lastname: Fadel
authors.forename: M
authors.initials: M
authors.lastname: Dellamano
authors.forename: J R
authors.initials: JR
authors.lastname: Olivieri
authors.forename: W F
authors.initials: WF
authors.lastname: de Azevedo
authors.forename: G O
authors.initials: GO
authors.lastname: Bonilla-Rodriguez
mesh_terms: D000818:Animals; D003460:Crystallization; D010108:Oxyhemoglobins / Q000737:chemistry*; D012399:Rotation; D019596:Wolves / Q000097:blood*; D014961:X-Ray Diffraction
chemical_list: D010108:Oxyhemoglobins