[A method for atraumatic erythrocyte plasma removal to obtain membranes capable of performing the hemostatic function of thrombocytes].
A method for physiological hemoglobin elimination from erythrocytes is proposed. Its characteristic feature is increase of erythrocyte membrane permeability and complete release of hemoglobin from erythrocytes by adding an equal volume of 2% papaverin hydrochloride to washed erythrocyte suspension, followed by washing of empty erythrocyte membranes in isotonic sodium chloride solution. Clean intact erythrocytic membranes are obtained, possessing the hemostatic functions typical of blood platelets.
Grigor'ev
G I
GI
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D003593:Cytoplasm; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D005260:Female; D006454:Hemoglobins / Q000187:drug effects / Q000302:isolation & purification*; D006461:Hemolysis / Q000187:drug effects; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged
D006454:Hemoglobins