Serotype and apx genotype profiles of Actinobacillus pleuropneumoniae field isolates in Korea.
A total of 100 field isolates of Actinobacillus pleuropneumoniae isolated from lung tissues of pigs with severe pleuropneumonia were serotyped by slide agglutination and precipitation tests. Polymerase chain reactions for apxICA, apxIICA, apxIIICA, apxIBD and apxIIIBD genes were used to determine their genotype prevalence. Serotypes 2 (56 isolates), 5 (28 isolates) and 6 (11 isolates) were the most common; only two isolates belonged to serotype 7, and three were untyped. Among the 97 isolates identified by serotype, 70 had the same apx genes as their respective serotype reference strains, but 27 did not have any of the apx genes present in the corresponding serotype reference strain. Among these 27 isolates, 10 were serotype 2, 12 were serotype 5, three were serotype 6 and two were serotype 7.
Min
K
K
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Suwon, Kyounggi-Do, Republic of Korea.
Chae
C
C
D000189:Actinobacillus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D006460:Hemolysin Proteins; D007723:Korea / Q000453:epidemiology; D011001:Pleuropneumonia / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
C085361:ApxII toxin, bacteria; C079721:ApxIII toxin protein, Actinobacillus; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D006460:Hemolysin Proteins; C087744:ApxI toxin, Bacteria