[Features of consequences of osmotic modification at different steps of cell heating].
The influence of media with different osmotic pressure (NaCl water solutions) on survival and permeability of Escherichia coli B/r and Escherichia coli Bs-1 cells heated up to 50, 52 and 60 degrees C was investigated. Hypotonic media increased, while hypertonic media, within a certain range of sodium chloride concentrations, decreased the damaging action of heating independently of the temperature. The effectiveness of thermoprotection was seen to increase, and the range of osmolyte concentrations, at which the highest effect of protection takes place, to move markedly towards higher concentrations of NaCl with increase in heating temperature. A certain relationship is suggested between the observed phenomenon and the osmotic homeostasis system of microorganisms under condition of thermogenic and tonic stress.
Morozov
I I
II
Medical Radiology Research Centre, Russian Academy of Medical Sciences, Obninsk.
Dergacheva
I P
IP
Anisimova
N S
NS
Morozova
G V
GV
Petin
V G
VG
D002463:Cell Membrane Permeability; D004926:Escherichia coli / Q000378:metabolism*; D006358:Hot Temperature; D009994:Osmolar Concentration; D018384:Oxidative Stress; D012965:Sodium Chloride
D012965:Sodium Chloride