The 105-kDa protein component of Bacillus cereus non-haemolytic enterotoxin (Nhe) is a metalloprotease with gelatinolytic and collagenolytic activity.
A sequence of 91 amino acids residues, probably starting from the N-terminal of the mature protein, was determined for the 105-kDa protein of the non-haemolytic enterotoxin of Bacillus cereus. The last part of this sequence was similar to parts of the N-terminal portions of two collagenases of Clostridium histolyticum and Clostridium perfringens. Zymography, with intact collagen fibril and gelatin as substrates, showed that the 105-kDa protein had collagenolytic and gelatinolytic activity. The 105-kDa protein also showed activity against a typical collagenase substrate, azocoll, and was inhibited by EDTA and 1,10-phenanthroline. We conclude that the 105-kDa protein is a collagenase.
Lund
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Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway. terje.lund@veths.no
Granum
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D000595:Amino Acid Sequence; D016863:Bacillaceae Infections / Q000382:microbiology; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D017364:Collagenases / Q000737:chemistry / Q000378:metabolism*; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005517:Foodborne Diseases / Q000382:microbiology; D018093:Gelatinases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
D004768:Enterotoxins; C040792:enterotoxin, Bacillus cereus; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases