title: Postprandial response of activated factor VII in elderly women depends on the R353Q polymorphism.
abstract: BACKGROUND
  Activated factor VII (FVIIa) is a very potent coagulant and may be a key determinant of the outcome of a cardiovascular event. The main determinants of FVIIa are the R353Q polymorphism and dietary fat intake, which may have an interactive effect.
  
  
  OBJECTIVE
  The objective was to investigate whether the response of FVIIa to a fat-rich breakfast varies across genotypes of the R353Q polymorphism.
  
  
  METHODS
  Ninety-one apparently healthy elderly women (>60 y of age), 56 with the RR genotype and 35 with the RQ or QQ genotype, participated in a randomized, controlled crossover study. Subjects received 5 breakfasts, each on a separate day: 1 low-fat control breakfast and 4 high-fat test breakfasts. Blood samples were taken for measurement of FVIIa at 0800 before each breakfast (fasting) and at 1300 and 1500.
  
  
  RESULTS
  The mean (+/-SD) fasting FVIIa concentration was 93.3 +/- 26.7 U/L in women with the RR genotype, 49.3 +/- 19.1 U/L in those with the RQ genotype and 39.5 +/- 17.2 U/L in those with the QQ genotype. The mean absolute response to all 4 test breakfasts was 37.0 U/L in those with the RR genotype and 16. 1 U/L in those carrying the Q allele (P < 0.001 for difference). Likewise, the FVIIa response relative to fasting FVIIa was significantly higher in women homozygous for the R allele.
  
  
  CONCLUSIONS
  This observation may indicate a considerable difference in cardiovascular risk between genotype groups as a result of an increase in FVIIa after a fat-rich diet.
authors.affiliation: Department of Human Nutrition and Epidemiology, Agricultural University Wageningen, Wageningen, Netherlands.
authors.forename: L I
authors.initials: LI
authors.lastname: Mennen
authors.forename: M P
authors.initials: MP
authors.lastname: de Maat
authors.forename: G
authors.initials: G
authors.lastname: Meijer
authors.forename: P
authors.initials: P
authors.lastname: Zock
authors.forename: D E
authors.initials: DE
authors.lastname: Grobbee
authors.forename: F J
authors.initials: FJ
authors.lastname: Kok
authors.forename: C
authors.initials: C
authors.lastname: Kluft
authors.forename: E G
authors.initials: EG
authors.lastname: Schouten
mesh_terms: D000368:Aged; D018592:Cross-Over Studies; D004247:DNA / Q000737:chemistry; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D015942:Factor VIIa / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005260:Female; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D019518:Postprandial Period / Q000502:physiology*; D012044:Regression Analysis
chemical_list: D004041:Dietary Fats; D004247:DNA; D015942:Factor VIIa