Analysis of urinary nitric oxide metabolites in healthy subjects.
Nitric oxide (NO) has divergent actions under physiological and pathological conditions. NO is rapidly decomposed to nitrite (NO2-) and nitrate (NO3-). Since these metabolites are stable, they are good indices of NO production under various conditions. In the present study, we measured NO2- and NO3- concentrations in the urine collected from 62 hospital controls and 504 healthy subjects by means of a new HPLC system combined with Griess reaction. NOx was the sum of NO2- and NO3-. There was no considerable inter-day variation in urinary NO metabolite levels, and there was close correlation between NO2-, NO3- and NOx values in spot urine obtained in the early morning and those in 24-h stored urine in hospital controls. Urinary NO metabolite levels, which were corrected by creatinine (Cr) excretion and expressed on a logarithmic scale, showed normal distribution and were independent of sex and age in healthy subjects. The normal ranges of urinary NO2-, NO3- and NOx levels were estimated as 17-72 micromol/g Cr, 1,023-2,818 pmol/g Cr, and 1,071-2,951 micromol/g Cr, respectively. We also found that urinary NO metabolite levels were lower than normal range in patients with various diseases.
Kurioka
S
S
Department of Medicine, Shimane Medical University, Izumo, Japan.
Koshimura
K
K
Sugitani
M
M
Murakami
Y
Y
Nishiki
M
M
Kato
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D001172:Arthritis, Rheumatoid / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003924:Diabetes Mellitus, Type 2 / Q000652:urine; D004355:Drug Stability; D005260:Female; D006801:Humans; D007027:Hypothalamic Diseases / Q000652:urine; D007674:Kidney Diseases / Q000652:urine; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000652:urine; D009569:Nitric Oxide / Q000652:urine*; D009573:Nitrites / Q000652:urine; D012016:Reference Values; D019810:Sodium Azide
D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D019810:Sodium Azide