A rapid sample preparation method for the HPLC determination of the opioid antagonist naltrexone in serum.
HPLC with UV and electrochemical detection has routinely been employed for the determination of the opioid antagonist naltrexone in serum. Sample preparation protocols range from liquid/liquid to solid phase extraction. The sample preparation described in this communication uses ultrafiltration as the mode of sample preparation prior to HPLC analysis. The method is accurate, precise and saves considerable time compared to previously published techniques.
Hurst
W J
WJ
Department of Comparative Medicine, M. S. Hershey Medical Center, PA, USA.
Zagon
I S
IS
Aboul-Enein
H Y
HY
D002851:Chromatography, High Pressure Liquid; D009271:Naltrexone / Q000097:blood*; D009292:Narcotic Antagonists / Q000097:blood*; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet; D014462:Ultrafiltration
D009292:Narcotic Antagonists; D012710:Serum Albumin, Bovine; D009271:Naltrexone