Study of the evolution of the placenta and fetal pancreas in the pathophysiology of growth retardation intrauterine due to restricted maternal diet.
BACKGROUND
Intrauterine growth retard (IUGR) continues to be a significant perinatology problem at the end of this century. The nature of the etiologic agent, the time when the attack occurred during pregnancy and its duration affect the type of IUGR.


OBJECTIVE
To study the evolution of fetal pancreas and placenta between the 18th and 21st day of pregnancy in rats submitted to maternal protein-calorie restriction.


METHODS
Randomized controlled trial on laboratory animal.


METHODS
Forty-one normoglycemic pregnant Wistar rats.


METHODS
Rats were divided into six experimental groups according to their access to food and date of cesarean section (18th or 21st day): control with free access to food; diet restricted to 25% introduced on 1st day of pregnancy; and diet restricted to 25% after the 3rd day of pregnancy.


METHODS
Newborn weight, placenta weight, histopathological study (morphological histochemistry).


RESULTS
Maternal protein-calorie malnutrition caused intrauterine growth retard (IUGR) after the 18th day of pregnancy. Dietary restriction did not interfere with the morphology of the fetal pancreas and the immunohistochemical study of the placenta showed that glycogen stores were decreased between the 18th and 21st day in the control group and in a diet restricted to 25% from the first day of pregnancy. Dietary restriction after the 3rd day of pregnancy led to low placental glycogen concentrations on the 18th day and disappearance on the 21st day.


CONCLUSIONS
The pathophysiology of IUGR due to maternal protein-calorie restriction in rats is related to lower placental weight and low placental glycogen stores.
Rudge
M V
MV
Discipline of Obstetrics, Faculdade de Medicina de Botucatu, UNESP, Brazil. rudge@laser.com.br
Gomes
C M
CM
Calderon
I de M
Ide M
Ramos
M D
MD
Abbade
J F
JF
de Oliveira
M G
MG
da Silva
M G
MG
D000818:Animals; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology* / Q000503:physiopathology*; D006003:Glycogen / Q000032:analysis; D010179:Pancreas / Q000196:embryology* / Q000473:pathology*; D010920:Placenta / Q000737:chemistry / Q000473:pathology*; D011247:Pregnancy; D011248:Pregnancy Complications; D011502:Protein-Energy Malnutrition; D051381:Rats; D017208:Rats, Wistar
D006003:Glycogen