Structure of the human adenovirus serotype 2 fiber head domain at 1.5 A resolution.
Adenovirus binds to its receptor via the head domain of its fiber protein. We have crystallized the adenovirus serotype 2 (subgroup C) receptor binding domain and solved the structure at 1.5 A resolution by the molecular replacement technique using the known adenovirus type 5 head structure. Included in the high-resolution model are 306 water molecules, five alternative side chain conformations, and individual anisotropic temperature factors for each atom. The overall structure of the serotype 2 head is very similar to its serotype 5 homologue, apart from differences in some of the flexible loops. All but subgroup B adenoviruses are believed to use the recently identified protein CAR (Coxsackievirus and adenovirus receptor) as receptor. By comparison of the two structures and sequence alignment of CAR binding and non-CAR binding serotype fiber heads, we discuss possible receptor binding sites and propose a receptor binding site in a crevice between two monomers on the side of the trimer. The structural basis of the extraordinary stability of the fiber head trimer is also discussed.
van Raaij
M J
MJ
c/o Institut Laue Langevin, 6 rue Jules Horowitz, Grenoble Cedex 9, 38000, France.
Louis
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N
Chroboczek
J
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Cusack
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S
D000260:Adenoviruses, Human / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics; D001665:Binding Sites; D002213:Capsid / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D004583:Electrons; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011991:Receptors, Virus / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D013696:Temperature; D014867:Water
D036022:Capsid Proteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; C085301:adenovirus receptor; C018315:hexon capsid protein, Adenovirus; D014867:Water