Characterization of local tissue damage evoked by Bothrops jararaca venom in the rat connective tissue microcirculation: an intravital microscopic study.
The aim of this study was to investigate the dynamic of the effects after topical application of Bothrops jararaca venom (BjV) on the microcirculation of the internal spermatic fascia of Wistar rats. The administration of 1 microg of BjV induced marked disturbances in capillaries and postcapillary venules, characterized by vasodilatation, fibrin clot formation, hemorrhagic lesions and alterations in the leukocyte-endothelial interactions. The installation and development of these effects occurred simultaneously. The application of higher dose of venom (10 microg) induced more intense effects, observed by the time-course of the beginning, evolution and intensity of the effects. The time-course of events suggests that an interaction of different effects occurs during the development of local symptoms following the exposure of tissues to BjV.
Farsky
S H
SH
Laboratório de Fisiopatologia, Instituto Butantan, São Paulo, Brazil. farskyp@uol.com.br
Gonçalves
L R
LR
Cury
Y
Y
D000287:Administration, Topical; D000818:Animals; D017837:Bothrops / Q000502:physiology*; D002196:Capillaries / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D006470:Hemorrhage / Q000139:chemically induced; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012910:Snake Venoms / Q000633:toxicity*; D013085:Spermatic Cord / Q000098:blood supply* / Q000187:drug effects; D013997:Time Factors
D012910:Snake Venoms