title: D-RNA molecules associated with subisolates of the VT strain of citrus tristeza virus which induce different seedling-yellows reactions.
abstract: Citrus tristeza virus (CTV) strains were previously catalogued as seedling-yellows (SY) and non-SY (nSY) types, according to their yellowing and stunting effects on indicator seedlings. Among subisolates of the VT strain, which were selected from chronically infected Alemow plants, there was a correlation between the presence of 2.4-, 2.7- and 4.5-kb D-RNAs, and SY and nSY reactions, respectively. Similarly, plants infected with Mor-T subisolates, which cause SY, contained D-RNAs of 2.6 to 2.8 kb, while nSY subisolates from recovered sour orange tissue contained a major D-RNA of 5.1 kb. Plants harboring the 2.7-kb D-RNA were protected against challenge inoculation with a subisolate harboring the 4.5-kb D-RNA. This study suggests that the nSY reaction results either from the absence of SY gene(s) in the genomes of certain CTV strains or through the suppression of the effects of SY gene(s) by D-RNAs with 5' parts larger than 4000nt.
authors.affiliation: The S. Tolkowsky Laboratory, Department of Virology, Agricultural Research Organization, the Volcani Center, Bet Dagan, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Che
authors.forename: R
authors.initials: R
authors.lastname: Gofman
authors.forename: Y
authors.initials: Y
authors.lastname: Ben-Shalom
authors.forename: D
authors.initials: D
authors.lastname: Piestun
authors.forename: R
authors.initials: R
authors.lastname: Gafny
authors.forename: M
authors.initials: M
authors.lastname: Mawassi
authors.forename: M
authors.initials: M
authors.lastname: Bar-Joseph
mesh_terms: D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000821:virology*; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral