Determination of glutathionyl hemoglobin in hemodialysis patients using electrospray ionization liquid chromatography-mass spectrometry.
We first detected glutathionyl hemoglobin (Hb) beta-chain in hemodialysis patients and healthy subjects using electrospray ionization liquid chromatography-mass spectrometry. The ratio of glutathionyl Hb beta-chain to total beta-chain was markedly increased in the hemodialysis patients as compared with healthy subjects. Glutathionyl Hb will be used as a new clinical marker of oxidative stress.
Naito
C
C
Nagoya University Daiko Medical Center, Japan.
Kajita
M
M
Niwa
T
T
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D018384:Oxidative Stress; D006435:Renal Dialysis
D015415:Biomarkers; D006454:Hemoglobins; D005978:Glutathione