Characterization of the phthalate permease OphD from Burkholderia cepacia ATCC 17616.
The ophD gene, encoding a permease for phthalate transport, was cloned from Burkholderia cepacia ATCC 17616. Expression of the gene in Escherichia coli results in the ability to transport phthalate rapidly into the cell. Uptake inhibition experiments show that 4-hydroxyphthalate, 4-chlorophthalate, 4-methylphthalate, and cinchomeronate compete for the phthalate permease. An ophD knockout mutant of 17616 grows slightly more slowly on phthalate but is still able to take up phthalate at rates equivalent to that of the wild-type strain. This means that 17616 must have a second phthalate-inducible phthalate uptake system.
Chang
H K
HK
Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA.
Zylstra
G J
GJ
D001426:Bacterial Proteins; D001692:Biological Transport; D016956:Burkholderia cepacia / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D010795:Phthalic Acids / Q000378:metabolism*
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D010795:Phthalic Acids; C121797:ophD protein, Burkholderia cepacia