title: First chromatographic isolation of an antifungal thaumatin-like protein from French bean legumes and demonstration of its antifungal activity.
abstract: A protein, with a molecular weight of 20 kDa, and an N-terminal sequence analogous to those of thaumatin-like proteins (TLPs) and thaumatins, was first isolated from the legume of the French bean Phaseolus vulgaris cv Kentucky wonder using a simple procedure involving affinity and ion exchange chromatography. The protein was adsorbed on both CM-Sepharose and Affi-gel Blue Gel. It was the first leguminous TLP-like protein demonstrated to exert antifungal activity against Fusarium oxysporum, Pleurotus ostreatus, and Coprinus comatus but not against Rhizoctonia solani.
authors.affiliation: Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Ye
authors.forename: H X
authors.initials: HX
authors.lastname: Wang
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003302:Coprinus / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics; D005670:Fusarium / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal; D020076:Pleurotus / Q000187:drug effects; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013549:Sweetening Agents
chemical_list: D000935:Antifungal Agents; D010940:Plant Proteins; D013549:Sweetening Agents; C003427:thaumatin protein, plant