Isolation and characterization of macaque dendritic cells from CD34(+) bone marrow progenitors.
We have developed a method for isolating and characterizing pigtailed macaque dendritic cells (DCs) generated from CD34(+) bone marrow (BM) progenitors based on methods previously developed for isolating human DCs. Macaque DCs displayed a characteristic morphology and were potent stimulators of allogeneic T cell proliferation. They expressed a set of DC-associated markers, such as MHC class II, CD1a, CD4, CD11a, CD40, CD58, CD80, CD83, CD86, and CXCR4. Macaque DCs, as well as peripheral blood CD4(+) T cells, were highly susceptible to HIV-2 infection, as detected by DNA-PCR. The expression of HIV-2 in macaque DCs was downregulated by treatment with the beta-chemokine RANTES. Macaque DCs will be useful for defining the in vivo role of DCs in HIV pathogenesis and for optimizing and testing peptide-DC vaccines or tolerizing regimens.
Pinchuk
L M
LM
Regional Primate Research Center, University of Washington Medical Center, Seattle, Washington 98195, USA.
Grouard-Vogel
G
G
Magaletti
D M
DM
Doty
R T
RT
Andrews
R G
RG
Clark
E A
EA
D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000276:immunology* / Q000821:virology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D015498:HIV-2 / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000821:virology; D006801:Humans; D008254:Macaca nemestrina
D018952:Antigens, CD34