title: Bovine milk antibodies against cell surface protein antigen PAc-glucosyltransferase fusion protein suppress cell adhesion and alter glucan synthesis of Streptococcus mutans.
abstract: Cell surface protein antigen (PAc) and glucosyltransferases (GTF) produced by Streptococcus mutans are considered major colonization factors of the organism, and the inhibition of these factors is thought to prevent dental caries. In this study, 8-mo-old pregnant Holstein cows were immunized with fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region (PAcA) of PAc with the glucan binding (GB) domain of GTF-I, an enzyme catalyzing the synthesis of water-insoluble glucan from sucrose. High titers of immunoglobulin antibodies specific for the fusion protein were found in normal milk after reimmunization, and they persisted for approximately 3 mo. The immunoglobulin G (IgG) antibodies against PAcA-GB were purified from immunized milk. The antibodies significantly inhibited the adhesion of S. mutans cells to saliva-coated hydroxyapatite beads. IgG antibodies purified from immunized milk also inhibited total glucan synthesis by cell-associated GTF preparation and GTF-I from S. mutans. The immunized milk may be useful as a means of passive immunization for the prevention of dental caries in humans.
authors.affiliation: Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka 812-8582, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oho
authors.forename: Y
authors.initials: Y
authors.lastname: Shimazaki
authors.forename: M
authors.initials: M
authors.lastname: Mitoma
authors.forename: M
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Okano
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: H
authors.initials: H
authors.lastname: Kawagoe
authors.forename: M
authors.initials: M
authors.lastname: Fukuyama
authors.forename: N
authors.initials: N
authors.lastname: Fujihara
authors.forename: T
authors.initials: T
authors.lastname: Koga
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000627:therapeutic use*; D000954:Antigens, Surface / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D003731:Dental Caries / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005936:Glucans / Q000096:biosynthesis* / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D007116:Immunization, Passive; D037102:Lectins; D008892:Milk / Q000276:immunology*; D011247:Pregnancy; D013295:Streptococcus mutans / Q000276:immunology* / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; C037937:glucan-binding proteins; D005964:Glucosyltransferases