Partially degradable film/fabric composites: textile scaffolds for liver cell culture.
In this study, a composite scaffold combining textile superstructures and biomimetic glycopolymers is introduced, which may allow engineering of organotypic liver tissue in vitro. Woven poly(ethylene therephtalat) (PET) fabrics were coated on one side with a thin biodegradable polymer film (poly[D-L-lactic-co-glycolic acid] PLGA), in order to obtain a polar structure. The composite structure ensured the stability of the membrane during in vitro degradation, independently of mesh size. Matrix porosity increased when a polymer blend matrix was used. For hepatocyte culturing studies, the scaffolds were additionally coated with an artificial glycopolymer (poly[N-p-vinylbenzyl-D-lactoamide], PVLA) in order to improve cell attachment. It was observed that formation of aggregates depends on the scaffold geometry as well as on the pretreatment and medium conditions. After 4 days in culture, the pores of the fabric were filled with aggregates illustrating the possibility of immobilizing hepatocyte aggregates in well-defined spatial configurations on textile structures.
Karamuk
E
E
Biocompatible Material Science and Engineering, Department of Materials, Swiss Federal Institute of Technology, Zürich. karamuk@biocomp.mat.ethz.ch
Mayer
J
J
Wintermantel
E
E
Akaike
T
T
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002449:Cell Aggregation; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003470:Culture Media; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D007785:Lactose / Q000031:analogs & derivatives / Q000737:chemistry; D008099:Liver / Q000166:cytology*; D008567:Membranes, Artificial; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D016062:Porosity; D013499:Surface Properties; D013784:Textiles
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D003470:Culture Media; D008567:Membranes, Artificial; D011093:Polyethylene Terephthalates; D011108:Polymers; D011137:Polystyrenes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose