title: Resistance of ovine, caprine and bovine endothelial cells to Clostridium perfringens type D epsilon toxin in vitro.
abstract: Ovine, caprine and bovine endothelial cells were grown in vitro and challenged with Clostridium perfringens type D epsilon toxin to compare their susceptibility to this toxin. Madin Darby canine kidney (MDCK) cells, which are known to be susceptible to epsilon toxin, were used as a positive control. No morphological alterations were observed in any of the endothelial cell cultures tested, even after challenging with doses as high as 1200 MLD50/ml of epsilon toxin. MDCK cells showed contour rounding and nuclear condensation as early as 30 min after exposure to 100 MLD50/ml of epsilon toxin and after 60 min of exposure to 12.5 MLD50/ml of the same toxin. All the MDCK cells were dead after 3 h of exposure to all concentrations of epsilon toxin. The results indicate that ovine, caprine and bovine endothelial cells are not morphologically responsive to the action of epsilon toxin in vitro.
authors.affiliation: School of Veterinary Sciences and Animal Production, The University of Queensland, Brisbane, Australia.
authors.forename: F A
authors.initials: FA
authors.lastname: Uzal
authors.forename: B E
authors.initials: BE
authors.lastname: Rolfe
authors.forename: N J
authors.initials: NJ
authors.lastname: Smith
authors.forename: A C
authors.initials: AC
authors.lastname: Thomas
authors.forename: W R
authors.initials: WR
authors.lastname: Kelly
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001427:Bacterial Toxins / Q000633:toxicity*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D003016:Clostridium perfringens / Q000472:pathogenicity*; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D004767:Enterotoxemia / Q000382:microbiology*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D018613:Microscopy, Confocal / Q000662:veterinary; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin