title: Bilayered biodegradable poly(ethylene glycol)/poly(butylene terephthalate) copolymer (Polyactive) as substrate for human fibroblasts and keratinocytes.
abstract: The purpose of this study was to find an optimal polymer matrix and to optimize the culture conditions for human keratinocytes and fibroblasts for the development of a human skin substitute. For this purpose porous, dense bilayers made of a block copolymer of poly(ethylene glycol terephthalate) (PEGT) and poly(butylene terephthalate) (PBT; Polyactivetrade mark) with a PEGT/PBT weight ratio of 55/45 and a PEG molecular weight (MW) of 300, 600, 1000, or 4000 Da were used. The best performance was achieved with PEGT/PBT copolymer with MW of PEG 300 D (300PEG55PBT45). When fibroblasts were seeded into the porous underlayer and cultured for 3 weeks in medium supplemented with 100 microg/mL ascorbic acid, all pores were filled with fibroblasts and with extracellular matrix, which was judged from the presence of collagen types I, III, and IV, and laminin. When seeded onto the dense top layer of the bilayered (cell free or fibroblast populated) copolymer matrix, human keratinocytes grew out into confluent sheets. After subsequent lifting to the air-liquid interface, a multilayered epithelium with a morphology corresponding to that of the native epidermis was formed. Some differences could still be observed: the expression and localization of some differentiation specific proteins was different and close to that seen in hyperproliferative epidermis; a basal lamina and anchoring zone were absent.
authors.affiliation: Department of Dermatology, Leiden University Medical Center, The Netherlands.
authors.forename: A G
authors.initials: AG
authors.lastname: van Dorp
authors.forename: M C
authors.initials: MC
authors.lastname: Verhoeven
authors.forename: H K
authors.initials: HK
authors.lastname: Koerten
authors.forename: C A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.forename: M
authors.initials: M
authors.lastname: Ponec
mesh_terms: D001672:Biocompatible Materials / Q000493:pharmacokinetics*; D001673:Biodegradation, Environmental; D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000493:pharmacokinetics*; D011092:Polyethylene Glycols / Q000493:pharmacokinetics*; D012867:Skin / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D017768:Skin, Artificial
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C072932:polyethylene oxide-polybutylene terephthalate copolymer; D011092:Polyethylene Glycols