Towards the in vitro reconstitution of caveolae. Asymmetric incorporation of glycosylphosphatidylinositol (GPI) and gangliosides into liposomal membranes.
Large unilamellar vesicles consisting of phospholipids with or without cholesterol have been prepared containing GPI and/or gangliosides asymmetrically located in the outer leaflet of the bilayer. Such asymmetric distribution of GPI and gangliosides is found in 'rafts' and caveolae. Using these vesicles, GPI can be readily hydrolysed by phospholipases. Both cholesterol and ganglioside are seen to inhibit, in an additive way, the hydrolytic activity of GPI-specific phospholipase D.
Villar
A V
AV
Unidad de Biofísica (CSIC-UPV/EHU), Universidad del País Vasco, Bilbao, Spain.
Alonso
A
A
Pañeda
C
C
Varela-Nieto
I
I
Brodbeck
U
U
Goñi
F M
FM
D000818:Animals; D005732:Gangliosides / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008081:Liposomes / Q000138:chemical synthesis* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000502:physiology*; D009439:Neuraminidase / Q000378:metabolism; D010539:Permeability; D010739:Phospholipase D / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D000519:alpha-Galactosidase / Q000378:metabolism
D005732:Gangliosides; D017261:Glycosylphosphatidylinositols; D008081:Liposomes; C025447:sialogangliosides; D010739:Phospholipase D; D009439:Neuraminidase; D000519:alpha-Galactosidase