Fast assay and mini-purification of protein by high performance membrane affinity chromatography.
Several affinity ligands of protein A, human immunoglobulin G, iminodiacetate (IDA)-Cu2+, and cibacron blue F3GA were coupled to GMA-modified cellulose membrane matrices. We prepared the columns based on the membrane supports in high performance membrane affinity chromatography (HPMAC). Next we evaluated nonspecific adsorption of proteins on the membrane media. The rapid assay of proteins on the affinity columns based on membrane supports was achieved. It was observed that the affinity columns based on membrane supports provided good reproducibility, high separation efficiency, and low pressure drop of the column. The columns with the membrane media could also be used for purification of proteins.
Zhou
D
D
National Chromatography Research and Analysis Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences.
Zou
H
H
Ni
J
J
Yang
L
L
Jia
L
L
Zhang
Y
Y
D000327:Adsorption; D000818:Animals; D002482:Cellulose; D002846:Chromatography, Affinity / Q000379:methods*; D006041:Goats; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D008024:Ligands; D011506:Proteins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013205:Staphylococcal Protein A / Q000378:metabolism
D007074:Immunoglobulin G; D008024:Ligands; D011506:Proteins; D013205:Staphylococcal Protein A; D002482:Cellulose