[A candidate peptide reacting with cytotoxic T cells in paraneoplastic cerebellar degeneration with anti-Yo antibody].
Anti-Yo antibody is present in some patients with paraneoplastic cerebellar degeneration (PCD), but there is no evidence that it causes Purkinje cell loss. We found HLA A24 in all of the patients. The roles of HLA class I molecules in neuronal loss need to be examined. The peptide-binding groove of HLA class I molecules fits the peptides of 8-10 amino acids. Allele specificity has been shown in the interaction between peptide and HLA molecules. We examined HLA-class I restricted cytotoxic T cell (CTL) activity against autologous fibroblasts using a peptide of the Yo protein with the HLA A24-specific allele. The CTL activity in a patient showed 13.8% at effector/target ratio of 65:1. This is the first evidence of the presence of CTL reacting with a peptide of Yo protein in the peripheral venous blood from a patient with PCD.
Tanaka
M
M
Department of Neurology, National West Niigata Central Hospital, Japan.
Tanaka
K
K
D000328:Adult; D000906:Antibodies / Q000097:blood*; D001324:Autoantigens; D004268:DNA-Binding Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000276:immunology*; D009419:Nerve Tissue Proteins; D020362:Paraneoplastic Cerebellar Degeneration / Q000276:immunology*; D010455:Peptides / Q000097:blood; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000906:Antibodies; D001324:Autoantigens; C063762:CDR1 protein, human; C095184:CDR2 protein, human; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides