Analysis of myelodysplastic syndrome clones arising after multiple myeloma: a case study by correlative interphase cytogenetic analysis.
BACKGROUND
A patient with multiple myeloma developed myelodysplastic syndrome (MDS). Chromosomal analysis performed after the development of MDS revealed monosomy of chromosome 9 in all the meta-phases. We wished to identify the extent of the clone with the chromosomal abnormality originating from MDS clone.


METHODS
A correlative interphase study by fluorescence in situ hybridization (FISH) was performed and we determined whether each lineage of cells obtained the molecular mark. The chromosome 9 classic alpha satellite region DNA was used as a probe for the FISH analysis in smear specimens stained with Wright-Giemsa stain.


RESULTS
Erythroblasts, granulocytes and myelocytes had only one signal, whereas myeloma cells showed two to four signals.


CONCLUSIONS
This study visualized the spectrum of MDS clone. The results suggest that the origin of MDS is different from that of multiple myeloma, at least in this case.
Kobayashi
Y
Y
Department of Hematology/Oncology, Institute of Medical Science, The University of Tokyo, Japan. ykkobaya@gan2.ncc.go.jp
Nakayama
M
M
Uemura
N
N
Takeyama
K
K
Tobinai
K
K
Takenaka
T
T
Choi
S H
SH
Satoh
H
H
Mori
S
S
Asano
S
S
D002899:Chromosomes, Human, Pair 9; D002999:Clone Cells; D003582:Cytogenetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008875:Middle Aged; D009006:Monosomy; D009101:Multiple Myeloma / Q000150:complications* / Q000473:pathology; D009190:Myelodysplastic Syndromes / Q000209:etiology / Q000235:genetics*