Rise in intracellular calcium concentration by propylene glycol in PC12 cells.
In order to elucidate the mode of excitatory actions of propylene glycol (PG) on nervous tissues, intracellular calcium concentration ([Ca2+]i) of PC12 cells was measured on the video-imaging analysis system with fura-2. PG concentration-dependently (0.1-20%v/v) raised the [Ca2+]i. Hexamethonium and d-tubocurarine inhibited the carbamylcholine-induced [Ca2+]i rise, but these blockers had no effect on PG. High K+ potentiated the action of PG. The extent of the rise induced by PG in the differentiated cells was larger than that in the undifferentiated ones. The findings suggest that the rise in [Ca2+]i is involved in the excitatory effects of PG.
Hattori
T
T
Department of Dental Pharmacology, Matsumoto Dental University, Shiojiri, Japan. hattori@po.mdu.ac.jp
Maehashi
H
H
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D016257:Fura-2; D018738:Hexamethonium / Q000494:pharmacology; D009474:Neurons / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D018722:Nicotinic Agonists / Q000494:pharmacology; D018733:Nicotinic Antagonists / Q000494:pharmacology; D016716:PC12 Cells; D011188:Potassium / Q000494:pharmacology; D019946:Propylene Glycol / Q000494:pharmacology*; D051381:Rats; D011978:Receptors, Nicotinic / Q000502:physiology; D014403:Tubocurarine / Q000494:pharmacology
D003451:Cryoprotective Agents; D005456:Fluorescent Dyes; D018722:Nicotinic Agonists; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D018738:Hexamethonium; D019946:Propylene Glycol; D002217:Carbachol; D011188:Potassium; D002118:Calcium; D016257:Fura-2; D014403:Tubocurarine