title: Biomarkers of dose and susceptibility in cyclists exposed to monoaromatic hydrocarbons.
abstract: A quasi-experimental field study was carried out in 24 volunteers with the aim of: (i) assessing personal exposure to aromatic hydrocarbons polluting urban areas; and (ii) exploring the role of polymorphic enzymes relevant to the biotransformation of benzene in the inter-individual variability of biomarkers. Each subject covered by bicycle: (i) inner city routes with often jammed traffic; and (ii) open rural routes. Time-weighted average airborne concentrations of benzene, toluene, ethylbenzene and xylenes (BTEX) were determined during 2-h runs. BTEX were determined by solid-phase micro-extraction (SPME) followed by gas chromatography coupled with mass spectrometry (GC-MS) in blood and spot urine samples collected just before and immediately after the runs. Urinary t,t-muconic acid was measured by high performance liquid chromatography (HPLC)-UV. Genotypes of epoxide hydrolase (EH) and glutathione-S-transferase class mu-1 (GSTM1) were also characterised. As compared to pre-run values, benzene and toluene in blood, and toluene and xylenes in urine significantly increased after urban runs. Urinary t,t-muconic acid was significantly higher in post-run samples after both urban (P < 0.001) and rural runs (P < 0.05). Despite a narrow range of exposure levels, a significant relationship was observed between airborne benzene and post-run t,t-muconic acid (r2 = 0.349, P < 0.00). When subgroups were distinguished according to EH and GSTM, subjects bearing both the EH wild type and GSTM 'null' genotype showed significant exposure-related changes in t,t-muconic acid excretion. Even at very low exposure levels, a 2-h bike run in a polluted urban environment may give rise to measurable changes in biomarkers of internal dose of selected aromatic hydrocarbons. Genetically-based metabolic differences may account for part of the inter-individual variability of biomarkers of exposure.
authors.affiliation: Laboratory of Industrial Toxicology, University of Parma Medical School, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bergamaschi
authors.forename: A
authors.initials: A
authors.lastname: Brustolin
authors.forename: G
authors.initials: G
authors.lastname: De Palma
authors.forename: P
authors.initials: P
authors.lastname: Manini
authors.forename: P
authors.initials: P
authors.lastname: Mozzoni
authors.forename: R
authors.initials: R
authors.lastname: Andreoli
authors.forename: S
authors.initials: S
authors.lastname: Cavazzini
authors.forename: A
authors.initials: A
authors.lastname: Mutti
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000032:analysis*; D001554:Benzene / Q000032:analysis; D001555:Benzene Derivatives / Q000097:blood; D001642:Bicycling; D015415:Biomarkers / Q000097:blood / Q000652:urine; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000032:analysis* / Q000097:blood; D008297:Male; D012423:Rural Health; D012737:Sex Factors; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000032:analysis; D014050:Toluene / Q000097:blood; D014504:Urban Health; D014992:Xylenes / Q000097:blood
chemical_list: D000393:Air Pollutants; D001555:Benzene Derivatives; D015415:Biomarkers; D006841:Hydrocarbons, Aromatic; D014992:Xylenes; D014050:Toluene; C005939:muconic acid; D001554:Benzene; C004912:ethylbenzene; D013011:Sorbic Acid