Spatial arrangement of pig MHC class I sequences.
Bacterial artificial chromosome (BAC) clones were assigned within the pig major histocompatibility complex (Mhc) by polymerase chain reaction-screening and Southern blot hybridization using sequence-tagged site (STS) markers and BAC end-rescued sequences. In all, 35 BAC clones were discovered containing 12 anchor genes of the SLA class I region and two genes of the SLA class III region. Twenty of these 35 clones comprised two distinct class I gene clusters, each spanning about 100 kilobases. One cluster enclosed three class I related genes (SLA-6 to -8) and two genes (MIC-1 and MIC-2) more distantly related to class I. The other cluster enclosed typical class I genes, of which three (SLA-1, -2, and -3) were transcribed by fibroblasts homozygous for the H01 haplotype which we used to construct a pig BAC library. Ordered clones are certainly helpful in isolating agronomically, biologically, and medically important genes. They would also be useful for inducing genetic modifications in pig cell lines.
Velten
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Laboratoire Mixte INRA-CEA de Radiobiologie Appliquée, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France. velten@bafm.de
Renard
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Rogel-Gaillard
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Vaiman
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Chardon
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D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002876:Chromosomes, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005347:Fibroblasts / Q000378:metabolism; D015723:Gene Library; D005805:Genes, MHC Class I; D005822:Genetic Vectors / Q000235:genetics; D006084:Graft Rejection / Q000517:prevention & control; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics* / Q000276:immunology; D013556:Swine, Miniature / Q000235:genetics; D014158:Transcription, Genetic; D014183:Transplantation, Heterologous / Q000276:immunology
D011993:Recombinant Fusion Proteins