Interleukin-1beta causes a biphasic response in neurons of rat major pelvic ganglia.
The effect of interleukin-1beta (IL-1beta) on peripheral autonomic neurons was examined with intracellular microelectrodes, in vitro. Recombinant human IL-1beta (6-300 pM) produced a depolarization, associated with decrease in input resistance, followed by a hyperpolarization, associated with increase in input resistance, in neurons of rat major pelvic ganglia (MPG). IL-1beta 163-171 (10-100 pM), the active domain of human IL-1beta, also produced a biphasic response. The IL-1beta-induced responses reversed polarity at the equilibrium potential for Cl-. The IL-1beta-induced responses were blocked by picrotoxin (100 microM) but not by bicuculline (20 microM). Imidazole-4-acetic acid (14AA, 100 microM), a GABA(C) receptor antagonist, reduced the IL-1beta-induced responses. The results suggest that the IL-1beta-induced biphasic response is mediated through GABA(C) receptors in rat MPG neurons.
Akasu
T
T
Department of Physiology, Kurume University School of Medicine, Japan. akasut@med.kurume-u.ac.jp
Tsurusaki
M
M
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D005725:Ganglia, Autonomic / Q000187:drug effects* / Q000502:physiology*; D007001:Hypogastric Plexus / Q000187:drug effects* / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D001640:Bicuculline; D018756:GABA Antagonists; D007375:Interleukin-1; D010446:Peptide Fragments; D010852:Picrotoxin