title: A polymerase chain reaction protocol for the detection of various geographical isolates of white spot virus.
abstract: Polymerase chain reaction (PCR) primers were designed based on the sequence of a cloned fragment of the white spot virus (WSV) genome and were used to detect at least four geographic isolates of WSV from both experimentally- and naturally-infected shrimp. In addition to high specificity, the one-step and two-step PCR protocols were determined to have sensitivities of 10-100 pg and 100 femtograms respectively. The two-step PCR protocol is recommended as a very sensitive and specific alternative protocol to Western blot assay for the detection of WSV.
authors.affiliation: National Institute of Molecular Biology and Biotechnology, University of the Philippines Los Baños College, Laguna.
authors.forename: L M
authors.initials: LM
authors.lastname: Tapay
authors.forename: E C
authors.initials: EC
authors.lastname: Nadala
authors.forename: P C
authors.initials: PC
authors.lastname: Loh
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification; D033364:Decapoda / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004279:DNA, Viral