Distribution of extracellular matrix components in the developing ruminant corpus luteum: a wound repair hypothesis for luteinization.
The aim of this study was to investigate corpus luteum development by visualization of extracellular matrix proteins in the tissue at sequential stages of the luteal phase. Corpora lutea were collected from oestrus-synchronized sheep and from bovine material from an abattoir. The distributions of collagen types I and IV, fibronectin and von Willebrand factor were determined using immunohistology and semi-quantitative image analysis. During the post-ovulatory period, a fibronectin- and von Willebrand factor-rich matrix occurred centrally, adjacent to the inner parenchymal surface, whereas during early luteal development a clear border of fibronectin separated the inner parenchyma from the lumen. The inner parenchyma had abundant fibronectin initially, but the amount decreased as the rate of organ growth decreased. Over the same period, the amount of collagen type I first increased and then decreased. Collagen type I and fibronectin were less abundant in other regions of the parenchyma, and the general pattern was of slightly increasing amounts of collagen type I and decreasing amounts of fibronectin as luteal development proceeded. In contrast to earlier studies, only a small percentage of large luteal cells was found to have an associated layer of collagen type IV (presumed basal lamina). It is concluded that luteal growth and maturation require organized sequences of tissue remodelling. The central meshwork of fibronectin and von Willebrand factor and sequential deposition of collagen type I and fibronectin are strongly reminiscent of events in granulation tissue. This indicates that luteinization may be best understood as a wound repair-like process that succeeds the inflammation-like events of ovulation.
Silvester
L M
LM
Division of Animal Physiology, School of Biological Sciences, University of Nottingham, Loughborough, Leicestershire, UK.
Luck
M R
MR
D000818:Animals; D002417:Cattle / Q000502:physiology*; D016009:Chi-Square Distribution; D003094:Collagen / Q000032:analysis; D003338:Corpus Luteum / Q000737:chemistry / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008183:Luteal Phase / Q000502:physiology*; D008954:Models, Biological; D012756:Sheep; D014945:Wound Healing / Q000502:physiology*; D014841:von Willebrand Factor / Q000032:analysis
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D014841:von Willebrand Factor; D003094:Collagen