Induction of HSV-gD2 specific CD4(+) cells in Peyer's patches and mucosal antibody responses in mice following DNA immunization by both parenteral and mucosal administration.
A DNA vaccine encoding glycoprotein D (gD) of herpes simplex virus type 2 (pHSV-gD2) was injected via parenteral and mucosal routes to determine the optimal route of delivery for immune stimulation. Generation of distal mucosal immunity following parenteral vaccination was also evaluated. While all routes of DNA vaccine administration resulted in systemic cellular and humoral responses, the intra-muscular (i.m.) and intra-dermal (i.d.) routes of delivery produced the highest responses. Furthermore, i.m. and i.d. routes produced mucosal humoral responses that were comparable to those obtained via mucosal routes. Specific pHSV-gD2 PCR signals were detected in the Peyer's patches (PP) within hours following vaccination and antigen specific IgA was detected in secretions and supernatants from gut fragment cultures. Furthermore, antigen specific CD4(+) cells were found in PP. Collectively these results suggest that the DNA vaccine stimulated a response in the PP, a major inductive site for mucosal responses.
Shroff
K E
KE
Research and Development, Wyeth-Lederle Vaccines, Wyeth Ayerst Laboratories, One Great Valley Parkway, Malvern, PA 19355-1423, USA. shroffk@war.wyeth.com
Marcucci-Borges
L A
LA
de Bruin
S J
SJ
Winter
L A
LA
Tiberio
L
L
Pachuk
C
C
Snyder
L A
LA
Satishchandran
C
C
Ciccarelli
R B
RB
Higgins
T J
TJ
D000281:Administration, Intranasal; D000282:Administration, Intravaginal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D007114:Immunization; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000276:immunology; D010581:Peyer's Patches / Q000276:immunology*; D018139:Simplexvirus / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C040570:glycoprotein D-herpes simplex virus type 2