[Comparative cytotoxic characteristics of the interferon-inducing yeast RNA-tilorone complex].
Cytotoxicity of the yeast RNA-tilorona molecular complex (MC) with interferonogenic properties and its influence on the DNA replicative synthesis were studied in experiments with human lymphocytes and 3 cell lines. It was shown that the MC doses of 25, 100 and 250 micrograms/ml were absolutely nontoxic for all the cell lines. The main parameters of the MC toxicity based on the cell viability were calculated. The parameters were found to correlate in the order of their magnitude with those relating to interferonogens of the polynucleotide nature. Within the dose ranges of 10 to 100 micrograms/ml the MC had a stimulating effect on replicative processes in the cells. It was concluded that the use of the MC as an inductor in large-scale manufacture of human and animal interferons of type 1 was promising.
Zholobak
N M
NM
D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of the Ukraine, Kiev.
Karpov
A V
AV
Rybalko
S L
SL
Antonenko
S V
SV
Barbasheva
E V
EV
D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D012331:RNA, Fungal / Q000737:chemistry*; D013994:Tilorone / Q000737:chemistry*
D007369:Interferon Inducers; D012331:RNA, Fungal; D013994:Tilorone