title: A gene family encoding glutathione peroxidase homologues in Hordeum vulgare (barley).
abstract: We have isolated and characterised three barley cDNAs encoding glutathione peroxidase (GPX) homologues, designated HVGPH1, HVGPH2 and HVGPH3. HVGPH1 may represent a cytosolic form of GPX. The structure of the HVGPH2 N-terminal domain is typical for a plastid transit peptide. A potential peroxisomal targeting sequence occurs near the N-terminus of HVGPH3. Transcript levels of HVGPH1 and HVGPH2 were increased in leaves undergoing stress. In contrast, HVGPH3 mRNA accumulation showed a negative response to stress. Our data indicate that the barley genome bears a small gene family encoding GPX homologues differing in their function and cellular localisation.
authors.affiliation: Institute of Biology, Division of Genetics, Humboldt-University, Chausseestr. 117, D-10115, Berlin, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Churin
authors.forename: S
authors.initials: S
authors.lastname: Schilling
authors.forename: T
authors.initials: T
authors.lastname: Börner
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D017343:Genes, Plant; D005979:Glutathione Peroxidase / Q000235:genetics*; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D005979:Glutathione Peroxidase