title: Transient expression of the beta-glucuronidase gene in tissues of Arabidopsis thaliana by bombardment-mediated transformation.
abstract: Particle bombardment has proved to be useful for the transformation of plants. We have previously reported successful transient expression of the beta-glucuronidase (GUS) gene in cultured plant cells and tissues and the stable transformation of various plants using a pneumatic particle gun. In this chapter, we describe transient expression of the GUS gene in Arabidopsis thaliana leaves and roots using the pneumatic particle gun.
authors.affiliation: Laboratory of Plant Molecular Biology, Instutuite of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, Ibraki, Japan. mseki@rtc.riken.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Seki
authors.forename: A
authors.initials: A
authors.lastname: Iida
authors.forename: H
authors.initials: H
authors.lastname: Morikawa
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D019470:Biolistics; D005966:Glucuronidase / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005966:Glucuronidase