A model of suture-induced urolithiasis with urographic control in the bladder of the rat.
The characteristics of a model of urolithiasis in the bladder of male Wistar rats were evaluated. Two techniques were compared: one knot of suture material through the bladder wall, or 15-throw knots inside the bladder. Rats were randomly assigned to one of six groups, according to the technique and suture material used (polyester, silk, or chromic catgut). An excretory urogram was performed at days 30, 60, and 90. At the moment of positive radiographic diagnosis, animals were sacrificed and stones were obtained. All rats were sacrificed at day 90 regardless of the x-ray results. Variables evaluated were average days to a positive urogram, average weight of all uroliths, and percentage of animals developing urolithiasis throughout the study. There were no statistical differences between groups for the average weight of stones, which ranged from 0.008 to 1.502 g for individual cases. All three materials were more lithogenic with the 15-throw knot inside technique. No significant differences were detected for lithogenesis criteria between suture materials. X-ray dispersion spectroscopy evidenced calcium phosphate composition of two stones from chromic catgut, and magnesium phosphate composition of two stones from chromic catgut, two from silk, and two from polyester. Neo-epithelization over the suture was confirmed microscopically in bladders with the one-throw technique. We conclude that (1) this is an effective model of urolithiasis, with predictability in time, composition, and amount of stone formation; (2) the presence of more suture material inside the bladder is more lithogenic; and (3) excretory urograms are highly sensitive and specific for the noninvasive diagnosis of bladder urolithiasis.
Portilla
E
E
Surgical Research Division, Centro de Investigación Biomédica de Occidente Instituto Mexicano del Seguro Social, Guadalajara, Jal., Mexico. eportilla@mail.udg.mx
Ramos
A
A
Ramos
L
L
de Buen
N
N
García
D
D
Rodríguez-Reynoso
S
S
Larios
F
F
Terán
C
C
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013537:Sutures / Q000009:adverse effects*; D001743:Urinary Bladder / Q000473:pathology; D014545:Urinary Calculi / Q000000981:diagnostic imaging / Q000209:etiology*