The molecular cloning of 8-epicedrol synthase from Artemisia annua.
A cDNA library was prepared from Artemisia annua, and a 129-bp fragment was amplified from this library using primers corresponding to sequences conserved in known dicot sesquiterpene synthases. A 1641-bp open reading frame that encoded a predicted protein 35-38% identical to dicot sesquiterpene synthases was cloned using this fragment as a hybridization probe. The gene product expressed in Escherichia coli cyclized farnesyl diphosphate to a 96:4 mixture of (-)8-epicedrol and cedrol. Neither cedrol epimer was detected by GC-MS in an A. annua extract prepared from the same specimen as the cDNA.
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Department of Chemistry, Rice University, Houston, Texas, 77005, USA.
Matsuda
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D000595:Amino Acid Sequence; D000962:Antimalarials / Q000378:metabolism; D037621:Artemisinins; D019659:Asteraceae / Q000201:enzymology / Q000235:genetics*; D019755:Carbon-Carbon Lyases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D008956:Models, Chemical; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000201:enzymology / Q000235:genetics*; D000081223:Polycyclic Sesquiterpenes; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012717:Sesquiterpenes / Q000378:metabolism; D013729:Terpenes / Q000138:chemical synthesis* / Q000737:chemistry
D000962:Antimalarials; D037621:Artemisinins; D018076:DNA, Complementary; D000081223:Polycyclic Sesquiterpenes; D011106:Polyisoprenyl Phosphates; D018749:RNA, Plant; D011994:Recombinant Proteins; D012717:Sesquiterpenes; D013729:Terpenes; C078669:cedrol; C004808:farnesyl pyrophosphate; C031327:artemisinin; D019755:Carbon-Carbon Lyases; C122071:8-epicedrol synthase