title: IgA antibodies recognizing LABD97 are predominantly IgA1 subclass.
abstract: Linear IgA bullous dermatosis is a rare acquired subepidermal blistering disease of the skin. A recognized antigen in linear IgA bullous dermatosis is a 97-kDa basement membrane zone protein termed LABD97. Previous studies, using immunofluorescent techniques, have suggested that the IgA response is restricted to the IgA1 subclass. We studied the IgA antibody subclasses in the sera of 6 patients that contained circulating IgA antibodies reactive with LABD97. The methods used included direct and indirect immunofluorescence and Western immunoblot. All patients tested had IgA1 anti-LABD97 antibodies detected by all 3 methods. Two patients had IgA2 antibodies detected by direct immunofluorescence. Three patients had IgA2 antibodies on indirect immunofluorescence. Two of these also had anti-LABD97 IgA2 antibodies and 1 had secretory component containing anti-LABD IgA antibodies on Western immunoblot. We conclude that the predominant IgA antibody subclass reactive with LABD97 in LABD is IgA1, although the IgA2 subclass may be involved in some cases.
authors.affiliation: Department of Dermatology, University of Utah School of Medicine, Salt Lake City 84132, USA. C.A.Egan@m.cc.utah.edu
authors.forename: C A
authors.initials: CA
authors.lastname: Egan
authors.forename: M R
authors.initials: MR
authors.lastname: Martineau
authors.forename: T B
authors.initials: TB
authors.lastname: Taylor
authors.forename: L J
authors.initials: LJ
authors.lastname: Meyer
authors.forename: M J
authors.initials: MJ
authors.lastname: Petersen
authors.forename: J J
authors.initials: JJ
authors.lastname: Zone
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis* / Q000097:blood / Q000145:classification / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D015153:Blotting, Western; D002352:Carrier Proteins; D002675:Child, Preschool; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000097:blood / Q000145:classification / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D012867:Skin / Q000276:immunology* / Q000648:ultrastructure; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*; D000097805:Collagen Type XVII
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D007070:Immunoglobulin A; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human