title: [Enzymic activity of polymer-protein complexes in water-miscible organic solvents].
abstract: The enzymic activity of noncovalent complexes of alpha-chymotrypsin with polyethylene glycol and a block-copolymer of polyethylene oxide and polypropylene oxide (proxanol) was studied in aqueous-organic media. It was shown that complex formation activated the enzyme in media with a high content of the organic solvent, whereas in systems containing more than 50% water the enzymic activity of complexes was the same as that of the native enzyme. The activation in polyethylene glycol-containing complexes was greater than in complexes with proxanol of the same molecular mass.
authors.affiliation: Moscow State University, Chemical Faculty, Russia. iks@enzyme.chem.msu.su
authors.forename: I K
authors.initials: IK
authors.lastname: Sakodynskaya
authors.forename: E M
authors.initials: EM
authors.lastname: Sorokina
authors.forename: N V
authors.initials: NV
authors.lastname: Efremova
authors.forename: I N
authors.initials: IN
authors.lastname: Topchieva
mesh_terms: D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D011060:Poloxalene / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D011092:Polyethylene Glycols; D011060:Poloxalene; D002918:Chymotrypsin