Tissue-specific consequences of the anti-adenoviral immune response: implications for cardiac transplants.
The immune response to adenoviral vectors can induce inflammation and loss of transgene expression in transfected tissues. This would limit the use of adenovirus-mediated gene transfer in disease states in which long-term gene expression is required. While studying the effect of the anti-adenoviral immune response in transplantation, we found that transgene expression persisted in cardiac isografts transfected with an adenovirus encoding beta-galactosidase. Transfected grafts remained free of inflammation, despite the presence of an immune response to the vector. Thus, adenovirus-mediated gene transfer may have therapeutic value in cardiac transplantation and heart diseases. Furthermore, immunological limitations of adenoviral vectors for gene therapy are not universal for all tissue types.
Chan
S Y
SY
Section of General Surgery, Department of Surgery, University of Michigan School of Medicine, Ann Arbor, Michigan 48109-0654, USA.
Li
K
K
Piccotti
J R
JR
Louie
M C
MC
Judge
T A
TA
Turka
L A
LA
Eichwald
E J
EJ
Bishop
D K
DK
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000276:immunology*; D016027:Heart Transplantation / Q000009:adverse effects / Q000276:immunology*; D007262:Infusions, Intravenous; D051379:Mice; D008810:Mice, Inbred C57BL; D019076:Transgenes; D014185:Transplantation, Isogeneic; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase