title: Liquid chromatography-fast atom bombardment mass spectrometry for detection and determination of pentazocine in human tissues.
abstract: A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 microl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation.
authors.affiliation: Department of Legal Medicine, Hiroshima University School of Medicine, Japan. tohrui@ipc.hiroshima-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Imamura
authors.forename: K
authors.initials: K
authors.lastname: Kudo
authors.forename: A
authors.initials: A
authors.lastname: Namera
authors.forename: H
authors.initials: H
authors.lastname: Tokunaga
authors.forename: M
authors.initials: M
authors.lastname: Yashiki
authors.forename: N
authors.initials: N
authors.lastname: Jitsufuchi
authors.forename: T
authors.initials: T
authors.lastname: Kojima
mesh_terms: D000368:Aged; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D010423:Pentazocine / Q000032:analysis*; D015203:Reproducibility of Results; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
chemical_list: D010423:Pentazocine