[Membrane-filtration immunoassay: reagents, methods and the diagnostic and technical means for detection].
The comprehensive development of dot-EIA made at the State Research Institute of Biological Instrument-Making Industry has provided devices KIMF-02 and KIMF-03), a base of chemical reagents, immunoassays, test systems for detection of a wide range of causative agents of viral and bacterial infections, that of serodiagnosis of their related diseases. The KIMF-02 kit has undergone engineering and medical tests and recommended for the Ministry of Health of the Russian Federation to produce them in stock. The kit includes all required for analysis even in an ill-equipped laboratory, a set of attached agents ensures a valid visual recording of results. The developed procedures and test systems allow the immunoassay to be as sensitive as TIFA; however, they are laborious and much simpler in design. The simple and rapid procedures of dot-EIA are recommended for incorporation into the a package of laboratory methods for verification of the accumulation of virus-specific antigens in various biological substrata, environmental samples, for control of the activity of antigens and antibodies used in serological tests, for detection of specific antigens in the clinical samples, and for serodiagnosis of infections.
Khramov
E N
EN
Osin
N S
NS
Pomelova
V G
VG
Vikha
I V
IV
Bychenkova
T A
TA
Smirnova
V G
VG
Grakina
G I
GI
Kas'ianova
T A
TA
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D001424:Bacterial Infections / Q000175:diagnosis*; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D007202:Indicators and Reagents; D008567:Membranes, Artificial