title: Identification of a gene at 16q24.3 that restores cellular senescence in immortal mammary tumor cells.
abstract: We have mapped a cellular senescence gene, SEN16, within a genetic distance of 3 - 7 cM, at 16q24.3. Microcell mediated transfer of a normal human chromosome 16, 16q22-qter or 16q23-qter restored cellular senescence in four immortal cell lines, derived from human and rat mammary tumors. The resumption of indefinite cell proliferation, concordant with the segregation of the donor chromosome, confirmed the presence of a senescence gene at 16q23-qter. While microcell hybrids were maintained in selection medium to retain the donor chromosome, sporadic immortal revertant clones arose among senescent cells. Reversion to immortal growth could occur due to inactivation of the senescence gene either by a mutation or a deletion. The analysis for chromosome 16 specific DNA markers, in revertant clones of senescent microcell hybrids, revealed a consensus deletion, spanning a genetic interval of approximately 3 - 7 cM at 16q24.3.
authors.affiliation: Fels Institute for Cancer Research, 3307 North Broad Street, AHB Room 201, Temple University School of Medicine, Philadelphia, Pennsylvania, PA 19140, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Reddy
authors.forename: A K
authors.initials: AK
authors.lastname: Sandhu
authors.forename: J K
authors.initials: JK
authors.lastname: DeRiel
authors.forename: R S
authors.initials: RS
authors.lastname: Athwal
authors.forename: G P
authors.initials: GP
authors.lastname: Kaur
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002455:Cell Division / Q000235:genetics; D016922:Cellular Senescence / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D020161:Physical Chromosome Mapping; D051381:Rats; D014407:Tumor Cells, Cultured