title: Sequence analysis of the gene encoding VP4 of a bovine group C rotavirus: molecular evidence for a new P genotype.
abstract: Nucleotide sequence of the bovine group C rotavirus Shintoku strain gene 3 was determined. Segment 3 is 2253 nucleotides (nt) in length and contains a long open reading frame (ORF) beginning at nt 22 and terminating at nt 2223. This ORF encodes a polypeptide of 733 amino acids with a predicted molecular mass of 83 kDa. The deduced gene 3 amino acid sequence shares 79% and 73% identities with VP4 of the porcine Cowden and human Bristol strains, respectively. Lack of high amino acid sequence homology in VP4 of bovine, porcine, and human group C rotaviruses indicates that the Shintoku strain represents a new P genotype.
authors.affiliation: Viral Gastroenteritis Section, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, GA 30333, USA.
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.forename: J R
authors.initials: JR
authors.lastname: Gentsch
authors.forename: H
authors.initials: H
authors.lastname: Tsunemitsu
authors.forename: L J
authors.initials: LJ
authors.lastname: Saif
authors.forename: R I
authors.initials: RI
authors.lastname: Glass
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D002417:Cattle; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus