Structure determination of an exopolysaccharide from an alkaliphilic bacterium closely related to Bacillus spp.
An exopolysaccharide obtained from an alkaliphilic bacterium closely related to Bacillus spp. was found to contain D-galactopyranuronic acid (GalpA), 2,4-diacetamido-2,4,6-trideoxy-D-glucopyranose (QuipNAc4NAc), 2-acetamido-2-deoxy D-mannopyranuronic acid (ManpNAcA) and one uncommon unit of D-galactopyranuronic acid with the carboxyl group amide-linked to glycine [GalpA(Gly)]. The polysaccharide was studied by one-dimensional and two-dimensional 1H-NMR and 13C-NMR spectroscopy both on native polysaccharide and on monosaccharides and oligosaccharides obtained from methanolysis and from anhydrous HF solvolysis. The following linear structure of the repeating unit was established: -->3)-alpha-D-GalpA(Gly)-(1-->4)-beta-D-ManpNAcA-(1-->4)-alp ha-D-Galp A-(1-->3)-alpha-D-QuipNAc4NAc-(1-->. A preliminary phylogenetic assignment for the bacterium is also reported.
Corsaro
M M
MM
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.
Grant
W D
WD
Grant
S
S
Marciano
C E
CE
Parrilli
M
M
D001407:Bacillus / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D019143:Evolution, Molecular; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017421:Sequence Analysis
D004275:DNA, Ribosomal; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S