Bioreactor operated production of lipase: castor oil hydrolysis using partially-purified lipase.
A highly stable lipase from Pseudomonas aeruginosa KKA-5 was produced by batch cultivation technique employing shake flask and 5 L-bioreactor. The bioreactor was run at different airflow rates. Low airflow rates (1 and 3 L/min), did not lead to effective growth and lipase production. Growth increased by about one order and lipase production increased by about 6 times, at an airflow rate of 5 L/min. Lipase production occurred during decelerated cell growth. A highly stable lipase was produced which retained its activity in the running bioreactor, even after a period of one month. This stable lipase was partially-purified using ammonium sulphate precipitation technique. Castor oil was hydrolyzed using 300U crude and partially-purified lipase, each. Approximately 21-fold, partially-purified lipase could hydrolyze 81% castor oil within a period of 96 hr, where as only 63% hydrolysis was obtained, in 216 hour, when crude lipase was used.
Sharon
C
C
Dept. of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu-shi, Japan.
Nakazato
M
M
Ogawa
H I
HI
Kato
Y
Y
D019149:Bioreactors; D002368:Castor Oil / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D011550:Pseudomonas aeruginosa
D002368:Castor Oil; D008049:Lipase