title: The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae.
abstract: We have identified two Gcn5-dependent histone acetyltransferase (HAT) complexes from Saccharomyces cerevisiae, the 0.8-MDa ADA complex and the 1.8-MDa SAGA complex. The SAGA (Spt-Ada-Gcn5-acetyltransferase) complex contains several subunits which also function as part of other protein complexes, including a subset of TATA box binding protein-associated factors (TAFIIs) and Tra1. These observations raise the question of whether the 0.8-MDa ADA complex is a subcomplex of SAGA or whether it is a distinct HAT complex that also shares subunits with SAGA. To address this issue, we sought to determine if the ADA complex contained subunits that are not present in the SAGA complex. In this study, we report the purification of the ADA complex over 10 chromatographic steps. By a combination of mass spectrometry analysis and immunoblotting, we demonstrate that the adapter proteins Ada2, Ada3, and Gcn5 are indeed integral components of ADA. Furthermore, we identify the product of the S. cerevisiae gene YOR023C as a novel subunit of the ADA complex and name it Ahc1 for ADA HAT complex component 1. Biochemical functions of YOR023C have not been reported. However, AHC1 in high copy numbers suppresses the cold sensitivity caused by particular mutations in HTA1 (I. Pinto and F. Winston, personal communication), which encodes histone H2A (J. N. Hirschhorn et al., Mol. Cell. Biol. 15:1999-2009, 1995). Deletion of AHC1 disrupted the integrity of the ADA complex but did not affect SAGA or give rise to classic Ada(-) phenotypes. These results indicate that Gcn5, Ada2, and Ada3 function as part of a unique HAT complex (ADA) and represent shared subunits between this complex and SAGA.
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802-4500, USA.
authors.forename: A
authors.initials: A
authors.lastname: Eberharter
authors.forename: D E
authors.initials: DE
authors.lastname: Sterner
authors.forename: D
authors.initials: D
authors.lastname: Schieltz
authors.forename: A
authors.initials: A
authors.lastname: Hassan
authors.forename: J R
authors.initials: JR
authors.lastname: Yates
authors.forename: S L
authors.initials: SL
authors.lastname: Berger
authors.forename: J L
authors.initials: JL
authors.lastname: Workman
mesh_terms: D000123:Acetyltransferases / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000302:isolation & purification*; D017353:Gene Deletion; D005800:Genes, Fungal; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010641:Phenotype; D011494:Protein Kinases / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D020539:Sequence Analysis, Protein; D014157:Transcription Factors / Q000302:isolation & purification*; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D006657:Histones; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C090575:ADA2 protein, S cerevisiae; C403402:AHC1 protein, S cerevisiae; C083323:NGG1 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor