title: Molecular epidemiology of classical swine fever in Cuba.
abstract: The origin and evolution of the classical swine fever (CSF) epizootic that occurred in Cuba from 1993 to 1997 has been investigated by the analysis of E2 gene sequences from 15 representative viral isolates as well as the vaccine and the challenge strains used in this country. In the phylogenetic tree derived from these sequences, the Cuban isolates were located in a defined cluster within the previously reported genomic subgroup 1.2. This cluster was related, although distinguishable, from the live vaccine used in Cuba since 1965. Two further groups were identified. One of them included the early viruses isolated in the western part of Cuba until 1996 and the strain Margarita, used for vaccine potency tests since 1965. These results are consistent with the strain Margarita being the origin of the western outbreaks. The viruses isolated from 1996 in eastern Cuba defined a related, but independent group. The level of sequence variation observed in this group does not exclude an independent origin for the eastern isolates.
authors.affiliation: Centro Nacional de Sanidad Agropecuaria, apdo 10, San José de las Lajas, La Habana, Cuba.
authors.forename: H
authors.initials: H
authors.lastname: Díaz de Arce
authors.forename: J I
authors.initials: JI
authors.lastname: Núñez
authors.forename: L
authors.initials: L
authors.lastname: Ganges
authors.forename: M
authors.initials: M
authors.lastname: Barreras
authors.forename: M
authors.initials: M
authors.lastname: Teresa Frías
authors.forename: F
authors.initials: F
authors.lastname: Sobrino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006691:Classical Swine Fever / Q000453:epidemiology* / Q000503:physiopathology / Q000821:virology; D006692:Classical Swine Fever Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003462:Cuba / Q000453:epidemiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus