Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction.
Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype.
China
B
B
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Belgium. bchina@ulg.ac.be
Goffaux
F
F
Pirson
V
V
Mainil
J
J
D018829:Adhesins, Bacterial; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002352:Carrier Proteins; D002417:Cattle; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011956:Receptors, Cell Surface / Q000235:genetics; D014774:Virulence / Q000235:genetics
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; C102051:EspA protein, E coli; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; C073819:eaeA protein, E coli