Expression of osteopontin mRNA in the adult rat brain.
Examining the distribution of osteopontin (OPN) in the rat brain using in situ hybridization, it was found that OPN mRNA was restricted to likely neurons in the olfactory bulb and the brain stem; it was not detected in the telencephalon and the diencephalon. In addition, Northern blot analysis showed a confined expression only in the brain stem with higher level in the pons and the medulla than in the midbrain. In the brain stem, it was found in functionally diverse areas including motor-related areas, sensory system and reticular formation. This specific but widespread distribution suggests that OPN may play an important role in the adult rat brain.
Shin
S L
SL
Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Cha
J H
JH
Chun
M H
MH
Chung
J W
JW
Lee
M Y
MY
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D017403:In Situ Hybridization; D009474:Neurons / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism; D053495:Osteopontin; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000235:genetics*; D014018:Tissue Distribution
D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin