[Concentrating dopamine in liposomes using transmembrane gradient of ammonium sulfate].
The active loading of liposomes from egg phosphatidylcholine and cholesterol with dopamine using an ammonium sulfate gradient was studied. Our conditions allowed the enrichment of the monolamellar liposomes with 90% dopamine added to the medium and, thus, considerably improve the dopamine/lipid ratio. Dopamine-containing liposomes with tocopherol in their lipid bilayer were shown to be stable for 3 weeks.
Zhigal'tsev
I V
IV
Lomonosov State Academy of Fine Chemical Technology, Moscow, Russia.
Kolomeĭchuk
S N
SN
Kaplun
A P
AP
Kucherianu
V G
VG
Iurasov
V V
VV
Shvets
V I
VI
D000645:Ammonium Sulfate / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes; D010713:Phosphatidylcholines / Q000737:chemistry; D014810:Vitamin E / Q000737:chemistry
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D014810:Vitamin E; D002784:Cholesterol; D000645:Ammonium Sulfate; D004298:Dopamine