Regulation of divergent transcription from the uvrA-ssb promoters in Sinorhizobium meliloti.
The Sinorhizobium meliloti uvrA gene was isolated by complementation of a Rhodobacter sphaeroides UvrA- mutant. DNA sequencing of the region upstream of the S. meliloti uvrA gene reveals the presence of the ssb gene in the opposite transcriptional orientation. PCR-mediated mutagenesis demonstrated that expression of these two genes is inducible by DNA damage, and depends, in both cases, on the direct repeat GTTCN7GTTC (cited according to the direction of uvrA transcription). Comparison of the sequences of recA and uvrA promoters from different bacterial species of the alpha group of the Proteobacteria has identified the direct repeat GTTCYYKTTTTGTTC as the SOS box in this phylogenetic group.
Tapias
A
A
Department of Genetics and Microbiology, Faculty of Sciences, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Barbé
J
J
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D008937:Mitomycins / Q000494:pharmacology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011938:Rec A Recombinases / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D013014:SOS Response, Genetics; D016415:Sequence Alignment; D016962:Sinorhizobium meliloti / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008937:Mitomycins; D011993:Recombinant Fusion Proteins; C046000:mitomycin A; D011938:Rec A Recombinases; C068631:UvrA protein, E coli; D000251:Adenosine Triphosphatases