Gas chromatographic-tandem mass spectrometric quantification of human plasma and urinary nitrate after its reduction to nitrite and derivatization to the pentafluorobenzyl derivative.
Gas chromatography-mass spectrometry (GC-MS) of nitrite as its pentafluorobenzyl derivative in the negative-ion chemical ionization mode is a useful analytical tool to quantify accurately and sensitively nitrite and nitrate after its reduction to nitrite in various biological fluids. In the present study we demonstrate the utility of GC-tandem MS to quantify nitrate in human plasma and urine. Our present results verify human plasma and urine levels of nitrite and nitrate measured previously by GC-MS.
Tsikas
D
D
Institute of Clinical Pharmacology, Hannover Medical School, Germany. tsikas.dimitros@mh-hannover.de
Gutzki
F M
FM
Sandmann
J
J
Schwedhelm
E
E
Frölich
J C
JC
D005464:Fluorobenzenes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D009566:Nitrates / Q000097:blood / Q000737:chemistry* / Q000652:urine; D009573:Nitrites / Q000737:chemistry*; D005466:Fluorocarbons
D005464:Fluorobenzenes; D007202:Indicators and Reagents; D009566:Nitrates; D009573:Nitrites; C025063:pentafluorobenzyl bromide; D005466:Fluorocarbons