title: Transcription factor CP2 is crucial in hemoglobin synthesis during erythroid terminal differentiation in vitro.
abstract: The transcription factor CP2 was initially identified to bind to the promoter region of the murine alpha-globin gene and known to stimulate the expression of alpha-globin by increasing CP2 transcripts 3- to 5-fold during induced differentiation of mouse erythroleukemic (MEL) cells in vitro. Here, we report that this increment of CP2 expression is crucial in erythroid-specific globin gene expression and hemoglobin synthesis. When antisense CP2 was overexpressed in MEL cells, production of endogenous CP2 protein was reduced 70-80%, and significant loss of its promoter binding activity was observed. During HMBA-induced terminal differentiation of antisense CP2 expressing MEL cells, the transcription of endogenous alpha-globin gene was suppressed as expected. Moreover, both beta-globin gene expression and hemoglobin synthesis were also severely impaired, without affecting the expression of key heme enzyme genes or HMBA-induced proliferation and viability.
authors.affiliation: College of Natural Sciences, Hanyang University, Seoul, 133-791, Korea. hanyang.ac.kr
authors.forename: J H
authors.initials: JH
authors.lastname: Chae
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: C G
authors.initials: CG
authors.lastname: Kim
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D016375:Antisense Elements (Genetics); D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005786:Gene Expression Regulation; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D006397:Hematinics / Q000494:pharmacology; D006454:Hemoglobins / Q000096:biosynthesis*; D006801:Humans; D007438:Introns; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D011485:Protein Binding; D016601:RNA-Binding Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000081:Acetamides; D016375:Antisense Elements (Genetics); D004268:DNA-Binding Proteins; D006397:Hematinics; D006454:Hemoglobins; D016601:RNA-Binding Proteins; C501906:TFCP2 protein, human; C501905:Tfcp2 protein, mouse; D014157:Transcription Factors; D005914:Globins; C014026:hexamethylene bisacetamide