title: Dissociation between the state of leukocyte adhesiveness/aggregation in the peripheral blood and the availability of the CD11B/CD18 and CD62L antigens on the surface of the cells in patients with stress.
abstract: We adopted whole blood flow cytometry and direct labeling of the CD11b/CD18 and the CD62L antigens to study the relationship between their expression on the surface of peripheral leukocytes and the state of leukocyte adhesiveness/aggregation (LAA) as revealed by the leukergy test. We examined patients with infection/inflammation, acute stress and controls. The mean +/- S.D. channel fluorescence intensity of CD11b/CD18 antigen did not differ between patients with infection/inflammation (173 +/- 78) and controls (167 +/- 72). However, a significant (p < 0.0001) difference between these groups was noted regarding LAA state. There was a significant (p = 0.04) reduction in CD11b/CD18 in stress (135 +/- 60) and a significant (p < 0.001) increment in LAA. In both study groups, there was a significant reduction in CD62L. Patients were divided into those with CD11b/CD18 above and below the control's average. No correlation was found between the antigens and LAA. We assume that LAA in patients with stress state is CD11b/CD18 and CD62L independent.
authors.affiliation: Department of Internal Medicine D, Tel-Aviv Sourasky Medical Center, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Sasson
authors.forename: D
authors.initials: D
authors.lastname: Zeltser
authors.forename: O
authors.initials: O
authors.lastname: Rogowski
authors.forename: M
authors.initials: M
authors.lastname: Kassirer
authors.forename: R
authors.initials: R
authors.lastname: Rotstein
authors.forename: I
authors.initials: I
authors.lastname: Shapira
authors.forename: V
authors.initials: V
authors.lastname: Rudick
authors.forename: D
authors.initials: D
authors.lastname: Pevni
authors.forename: N
authors.initials: N
authors.lastname: Arber
authors.forename: A S
authors.initials: AS
authors.lastname: Berliner
mesh_terms: D000328:Adult; D018821:CD18 Antigens / Q000097:blood*; D016022:Case-Control Studies; D002448:Cell Adhesion; D002449:Cell Aggregation; D005260:Female; D006801:Humans; D007239:Infections / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D019041:L-Selectin / Q000097:blood*; D007962:Leukocytes / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013312:Stress, Physiological / Q000276:immunology*
chemical_list: D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D019041:L-Selectin