An assay for peptide binding to HLA-Cw*0102.
The assembly assay for peptide binding to class I major histocompatibility complex (MHC) molecules is based on the ability of peptides to stabilize MHC class I molecules synthesized by transporter associated with antigen processing (TAP)-deficient cell. The TAP-deficient cell line T2 has previously been used in the assembly assay to analyze peptide binding to HLA-A*0201 and -B*5101. In this study, we have extended this technique to assay for peptides binding to endogenous HLA-Cw*0102 molecules. We have analyzed the peptide binding of 20 peptides with primary anchor motifs for HLA-Cw*0102. One-third of the peptides analyzed bound with high affinity, half of the peptides examined did not bind, whereas the remaining peptides displayed intermediate binding activity. Interest in HLA-C molecules has increased significantly in recent years, since it has been shown that HLA-C molecules both can present peptides to cytotoxic T lymphocytes (CTL) and in addition are able to inhibit natural killer (NK)-mediated lysis.
Andersen
M H
MH
Department of Tumor Cell Biology, Danish Cancer Society, Copenhagen. mha@cancer.dk
Søndergaard
I
I
Zeuthen
J
J
Elliott
T
T
Haurum
J S
JS
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry / Q000378:metabolism; D002144:Callithrix; D002460:Cell Line; D002461:Cell Line, Transformed; D015236:HLA-C Antigens / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000378:metabolism
D015236:HLA-C Antigens; C403228:HLA-C*01:02 antigen; D010455:Peptides