title: Practical considerations in acquiring biological signals from confocal microscope.
authors.affiliation: Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors.forename: S K
authors.initials: SK
authors.lastname: Kong
authors.forename: S
authors.initials: S
authors.lastname: Ko
authors.forename: C Y
authors.initials: CY
authors.lastname: Lee
authors.forename: P Y
authors.initials: PY
authors.lastname: Lui
mesh_terms: D000814:Aniline Compounds; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D013696:Temperature; D014867:Water / Q000737:chemistry; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014966:Xanthenes; D014867:Water; C059715:Fluo-3; D004121:Dimethyl Sulfoxide