title: Gas chromatographic-tandem mass spectrometric quantification of human plasma and urinary nitrate after its reduction to nitrite and derivatization to the pentafluorobenzyl derivative.
abstract: Gas chromatography-mass spectrometry (GC-MS) of nitrite as its pentafluorobenzyl derivative in the negative-ion chemical ionization mode is a useful analytical tool to quantify accurately and sensitively nitrite and nitrate after its reduction to nitrite in various biological fluids. In the present study we demonstrate the utility of GC-tandem MS to quantify nitrate in human plasma and urine. Our present results verify human plasma and urine levels of nitrite and nitrate measured previously by GC-MS.
authors.affiliation: Institute of Clinical Pharmacology, Hannover Medical School, Germany. tsikas.dimitros@mh-hannover.de
authors.forename: D
authors.initials: D
authors.lastname: Tsikas
authors.forename: F M
authors.initials: FM
authors.lastname: Gutzki
authors.forename: J
authors.initials: J
authors.lastname: Sandmann
authors.forename: E
authors.initials: E
authors.lastname: Schwedhelm
authors.forename: J C
authors.initials: JC
authors.lastname: Frölich
mesh_terms: D005464:Fluorobenzenes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D009566:Nitrates / Q000097:blood / Q000737:chemistry* / Q000652:urine; D009573:Nitrites / Q000737:chemistry*; D005466:Fluorocarbons
chemical_list: D005464:Fluorobenzenes; D007202:Indicators and Reagents; D009566:Nitrates; D009573:Nitrites; C025063:pentafluorobenzyl bromide; D005466:Fluorocarbons