title: Preparation and characterization of microencapsulated proteinase inhibitor aprotinin.
abstract: Preparation of microcapsules through interfacial cross-linking of soluble starch/hydroxyethyl starch and bovine serum albumin (BSA) with terephthaloyl chloride is described. The proteinase inhibitor aprotinin, either native or active site protected, was microencapsulated, being incorporated in the aqueous phase. The influence of aqueous phase pH, BSA, and terephthaloyl chloride concentrations as well as stirring rate on microcapsule morphology and size was studied. The polycondensation pH was shown to be the determining factor for tough microcapsule production with a high encapsulation yield. The size of the microcapsules ranged between 10-30 and 50-100 microm at stirring speed 1500 and 500 rpm, respectively. Fourier transform infrared spectroscopic studies were performed on microcapsules prepared under various conditions. A correlation was established between spectral changes and microcapsule morphology and size. The optimal conditions for microcapsule degradation by alpha-amylase were found. Active site-protected aprotinin was shown to fully retain its activity after microencapsulation.
authors.affiliation: School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. nilar@enzyme.chem.msu.ru
authors.forename: N V
authors.initials: NV
authors.lastname: Larionova
authors.forename: N F
authors.initials: NF
authors.lastname: Kazanskaya
authors.forename: N I
authors.initials: NI
authors.lastname: Larionova
authors.forename: G
authors.initials: G
authors.lastname: Ponchel
authors.forename: D
authors.initials: D
authors.lastname: Duchene
mesh_terms: D000818:Animals; D007611:Aprotinin; D002214:Capsules; D002417:Cattle; D003432:Cross-Linking Reagents; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D006895:Hydroxyethyl Starch Derivatives; D008855:Microscopy, Electron, Scanning; D012710:Serum Albumin, Bovine
chemical_list: D002214:Capsules; D003432:Cross-Linking Reagents; D006895:Hydroxyethyl Starch Derivatives; D012710:Serum Albumin, Bovine; D007611:Aprotinin