title: Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on intestine 407 cells.
abstract: Aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. By immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kDa protein (p66) was identified as a receptor for A. sobria hemolysin on Intestine 407 cells. Treatment of p66 with N-glycosidase F reduced the apparent sized of p66 to 60 kDa on SDS-polyacrylamide gels. p66, released from Intestine 407 cells following incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, bound A. sobria hemolysin. Thus treatment of Intestine 407 cells with PI-PLC resulted in the remarkable decrease of the sensitivity to A. sobria hemolysin. These results are consistent with the hypothesis that p66, the binding protein for A. sobria hemolysin, is a glycosylphosphatidylinositol-anchored glycoprotein expressed on the surface of Intestine 407 cells and probably plays a role as a receptor for A. sobria hemolysin on the intestinal cells.
authors.affiliation: Department of Bacteriology, Institute of Tropical Medicine, Sakamoto, Nagasaki, 8528523, Japan.
authors.forename: A p
authors.initials: Ap
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Wada
authors.forename: K
authors.initials: K
authors.lastname: Yahiro
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: Y
authors.initials: Y
authors.lastname: Fujii
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuta
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: J
authors.initials: J
authors.lastname: Moss
authors.forename: T
authors.initials: T
authors.lastname: Hirayama
mesh_terms: D000333:Aeromonas / Q000737:chemistry*; D001427:Bacterial Toxins / Q000032:analysis / Q000378:metabolism; D002460:Cell Line / Q000737:chemistry / Q000187:drug effects; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000166:cytology; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D011233:Precipitin Tests; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D008565:Membrane Proteins; C091632:cell-surface retention-binding protein 1; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase