title: Prostaglandin E2 levels in blood plasma and in crevicular fluid of advanced periodontitis patients before and after surgical therapy.
abstract: OBJECTIVE
  The aim of the study was to determine PGE2 levels in venous blood plasma (VBP), gingival blood plasma (GBP) and gingival crevicular fluid (GCF) in advanced periodontal patients before and after 6 months surgical treatment.
  
  
  METHODS
  There were 12 patients aged 28-45 years and seven healthy subjects as controls included into the study. Clinical parameters such as PLI, GI, BI, PD, AL and bone height % from radiographs were recorded before and after treatment. PGE2 levels were determined by radioimmunoassay (125 I RIA Kit, NEN(R)).
  
  
  RESULTS
  Before treatment the mean VBP, GBP and GCF PGE2 levels in periodontal patients were higher than healthy controls (P < 0.001, Student's t-test). Six months after treatment the mean levels of PGE2 in VBP, GBP and GCF were significantly reduced in patients with improvement of clinical and roentgenological parameters (P < 0.001, Student's t-test).
  
  
  CONCLUSIONS
  It can be concluded that PGE2 is involved in the pathogenesis of periodontal disease. The inflamed periodontal tissues may produce significant amount of PGE2 and the degree of inflammation might be determined by the ratios of PGE2. The lower levels of PGE2 in blood plasma and in gingival crevicular fluid after treatment are signs of improvement of periodontal disease.
authors.affiliation: Department of Stomatology, Faculty of Medicine, University of Tartu, Tartu, Estonia.
authors.forename: E
authors.initials: E
authors.lastname: Leibur
authors.forename: A
authors.initials: A
authors.lastname: Tuhkanen
authors.forename: U
authors.initials: U
authors.lastname: Pintson
authors.forename: P O
authors.initials: PO
authors.lastname: Söder
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000601:surgery; D016025:Bone Transplantation; D016022:Case-Control Studies; D015232:Dinoprostone / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D008875:Middle Aged; D017063:Outcome Assessment, Health Care; D010518:Periodontitis / Q000097:blood / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D011863:Radioimmunoassay; D018709:Statistics, Nonparametric
chemical_list: D015232:Dinoprostone