title: Effect of pinacidil on the contractile response of cyclophosphamide-treated rat vas deferens.
abstract: Effect of pinacidil, a K+ channel opener, was studied on contractility of cyclophosphamide-treated rat vas deferens. The mean IC50 value of pinacidil against 1 mmol barium chloride induced rhythmic contractions and 40 mmol potassium chloride induced tonic contractions was significantly (P < 0.01 and P < 0.001, respectively) increased in the cyclophosphamide treated group as compared to the control. The mean EC50 value of norepinephrine (NE) in the presence of pinacidil (10(-6) mol) was significantly (P < 0.001) increased in the cyclophosphamide treated group. These findings indicate that the responsiveness of rat vas deferens smooth muscle to pinacidil is reduced following cyclophosphamide treatment.
authors.affiliation: Division of Pharmacology & Toxicology, Indian Veterinary Research Institute, Izatnagar.
authors.forename: A G
authors.initials: AG
authors.lastname: Reddy
authors.forename: V R
authors.initials: VR
authors.lastname: Prakash
authors.forename: H C
authors.initials: HC
authors.lastname: Tripathi
authors.forename: S K
authors.initials: SK
authors.lastname: Tandan
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism; D003520:Cyclophosphamide / Q000633:toxicity*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D020110:Pinacidil / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D014649:Vas Deferens / Q000187:drug effects* / Q000502:physiology
chemical_list: D018906:Antineoplastic Agents, Alkylating; D015221:Potassium Channels; D020110:Pinacidil; D003520:Cyclophosphamide; D002118:Calcium; D009638:Norepinephrine