Functional expression of hMYH, a human homolog of the Escherichia coli MutY protein.
We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885-3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mutY mutant and can remove A from an A. 8-hydroxydeoxyguanine mismatch and to a lesser extent can remove A from an A. G mismatch in vitro.
Slupska
M M
MM
Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
Luther
W M
WM
Chiang
J H
JH
Yang
H
H
Miller
J H
JH
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D020137:Base Pair Mismatch; D045647:DNA Glycosylases; D004260:DNA Repair; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D016296:Mutagenesis; D009699:N-Glycosyl Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
D003838:Deoxyadenine Nucleotides; D011994:Recombinant Proteins; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase