Beta-lactamase-free penicillin amidase from Alcaligenes sp.: isolation strategy, strain characteristics, and enzyme immobilization.
Isolation and characterization of a beta-lactamase (EC 3.5.2.6)-free, penicillin amidase (penicillin amidohydrolase, EC 3.5.1. 11)-producing organism is reported. The test strain was isolated by an enrichment technique with a substrate other than penicillins. The isolated strain belongs to the genus Alcaligenes. Phenylacetic acid was found to be the inducer of penicillin amidase. The amidase has a broad substrate spectrum. It is very active against penicillin G and semisynthetic cephalosporins, whereas penicillin V and semisynthetic penicillins acted moderately as a substrate. Immobilized cells of Alcaligenes sp. were shown to act as a reversible enzyme.
Pal
A
A
Department of Microbiology, Bose Institute, P1/12 C.I.T. Scheme VIIM, Calcutta 700 054, India.
Samanta
T B
TB
D000421:Alcaligenes / Q000145:classification / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D003470:Culture Media; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D010399:Penicillin Amidase / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature; D001618:beta-Lactamases / Q000378:metabolism*
D003470:Culture Media; D004800:Enzymes, Immobilized; D010648:Phenylacetates; D010399:Penicillin Amidase; D001618:beta-Lactamases; C025136:phenylacetic acid