title: High throughput fluorescent CE-SSCP SNP genotyping.
abstract: Large numbers of single nucleotide polymorphisms (SNPs) are being identified by several laboratories for the purpose of developing dense genetic maps. Single-strand conformation polymorphism (SSCP) analysis has been widely used as a method for detecting novel sequence variations in PCR products. Differences in migration of single-stranded DNA can be used not only to find mutations, but to genotype SNPs in large sample populations. Using PCR with fluorescent labeling and automated capillary electrophoresis SSCP (CE-SSCP), we have developed a panel of 15 functional candidate SNPs. With an automated single capillary instrument, relatively rapid and low cost CE-SSCP SNP genotyping using currently available technology is feasible for 135 000 genotypes per year. With parallel multiple array capillary electrophoresis, more genotypes per year may be attainable.
authors.affiliation: Laboratory for Developmental Neuroscience, Child and Adolescent Psychiatry, Department of Psychiatry, University of Chicago, MC3077, 5841 S Maryland Avenue, Chicago, Illinois 60637, USA. ed@yoda.bsd.uchi cago.edu
authors.forename: D
authors.initials: D
authors.lastname: Gonen
authors.forename: J
authors.initials: J
authors.lastname: Veenstra-VanderWeele
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: B
authors.initials: B
authors.lastname: Leventhal
authors.forename: E H
authors.initials: EH
authors.lastname: Cook
mesh_terms: D001331:Automation / Q000379:methods; D002874:Chromosome Mapping / Q000379:methods*; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods; D005456:Fluorescent Dyes; D005838:Genotype; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D011506:Proteins