Mass spectrometric study on the protein chemical modification of uremic patients in advanced Maillard reaction.
The Maillard reaction, initiated by the nonenzymatic reaction of reducing sugar with protein, is proposed to play a significant role in protein aging and the complications of aging and diabetes. In this study, we detected and quantified some advanced glycation endproducts (AGEs) in human serum proteins of control and uremic patients by a highly selective and specific assay, electrospray ionization liquid chromatography-mass spectrometry-mass spectrometry (ESI-LC-MS-MS). From our results, levels of each AGEs in serum of uremic patients were significantly elevated, compared to age-matched controls. These results provide the evidence for increased modifications of proteins by Maillard reaction in uremia.
Odani
H
H
Department of Internal Medicine, Nagoya University Daiko Medical Center, Japan.
Matsumoto
Y
Y
Shinzato
T
T
Usami
J
J
Maeda
K
K
D001798:Blood Proteins / Q000378:metabolism; D016022:Case-Control Studies; D003928:Diabetic Nephropathies / Q000097:blood; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D015416:Maillard Reaction; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D012015:Reference Standards; D014511:Uremia / Q000097:blood*
D001798:Blood Proteins; D017127:Glycation End Products, Advanced