In vitro kinetics of the rat brain succinate dehydrogenase inhibition by hexachlorophene.
Brain succinate dehydrogenase (SDH) activity was inhibited by in vitro hexachlorophene (HCP) with a half inhibitory concentration (IC50) of 0.65 x 10(-3) M. The HCP exerted noncompetitive inhibition at 0.5 mM (<IC50), uncompetitive inhibition at 0.65 mM (= IC50), and mixed-type inhibition at 0.8 mM (>IC50) on SDH activity. The brain SDH demands more energy of activation (deltaE) in the presence of HCP. The ionizable groups of SDH such as the sulfhydral group of cysteine and alpha-amino groups of cysteine were not altered qualitatively in the presence of HCP.
Lokanatha
V
V
Department of Zoology, Sri Venkateswara University, Tirupati, India.
Sailaja
P
P
Rajendra
W
W
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D006582:Hexachlorophene / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013696:Temperature
D000891:Anti-Infective Agents, Local; D013385:Succinate Dehydrogenase; D006582:Hexachlorophene