Detection of codon 12 mutation in the K-ras oncogene in pancreatic tumors.
Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenocarcinoma, three samples of pancreatic mucinous cystadenoma, and two samples without tumors in formalin-fixed paraffin embedded tissue sections. K-ras mutations at codon 12 were detected by a two-step PCR-enriched technique in all the samples of pancreatic adenocarcinoma, but not in cystadenoma or control samples. This technique may be useful for early detection of pancreatic cancer.
Kubrusly
M S
MS
Gastroenterology Surgery Department, Faculty of Medicine São Paulo University.
Matheucci Júnior
E
E
Leite
K R
KR
Coelho
A M
AM
Monte
O
O
Machado
M C
MC
Pinotti
H W
HW
D003062:Codon / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D012680:Sensitivity and Specificity
D003062:Codon; D004273:DNA, Neoplasm