title: Yeast Sec14p deficient in phosphatidylinositol transfer activity is functional in vivo.
abstract: Yeast phosphatidylinositol transfer protein (Sec14p) is essential for Golgi secretory function. It is widely accepted, though unproven, that phosphatidylinositol transfer between membranes represents the physiological activity of phosphatidylinositol transfer proteins (PITPs). We report that Sec14pK66,239A is inactivated for phosphatidylinositol, but not phosphatidylcholine (PC), transfer activity. As expected, Sec14pK66,239A fails to meet established criteria for a PITP in vitro and fails to stimulate phosphoinositide production in vivo. However, its expression efficiently rescues the lethality and Golgi secretory defects associated with sec14-1ts and sec14 null mutations. This complementation requires neither phospholipase D activation nor the involvement of a novel class of minor yeast PITPs. These findings indicate that PI binding/transfer is remarkably dispensable for Sec14p function in vivo.
authors.affiliation: Department of Cell Biology, University of Alabama at Birmingham 35294-0005, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Phillips
authors.forename: B
authors.initials: B
authors.lastname: Sha
authors.forename: L
authors.initials: L
authors.lastname: Topalof
authors.forename: Z
authors.initials: Z
authors.lastname: Xie
authors.forename: J G
authors.initials: JG
authors.lastname: Alb
authors.forename: V A
authors.initials: VA
authors.lastname: Klenchin
authors.forename: P
authors.initials: P
authors.lastname: Swigart
authors.forename: S
authors.initials: S
authors.lastname: Cockcroft
authors.forename: T F
authors.initials: TF
authors.lastname: Martin
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: V A
authors.initials: VA
authors.lastname: Bankaitis
mesh_terms: D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D006860:Hydrogen Bonding; D007700:Kinetics; D008565:Membrane Proteins; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010710:Phosphates / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002352:Carrier Proteins; D005960:Glucosides; D008565:Membrane Proteins; D010710:Phosphates; D019869:Phosphatidylinositol 3-Kinases; D010716:Phosphatidylinositols; D049294:Phospholipid Transfer Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C483438:SEC14 protein, S cerevisiae; C118289:SEC24 protein, S cerevisiae; C018619:octyl-beta-D-glucoside