Cell and protein adhesion studies in glaucoma drainage device development. The AGFID project team.
OBJECTIVE
To examine in vitro whether phosphorylcholine coating of poly(methylmethacrylate) can reduce the adhesion of fibrinogen, fibrin, human scleral fibroblast and macrophage compared with current biomaterials used in the construction of glaucoma drainage devices.


METHODS
Sample discs (n=6) of poly(methylmethacrylate), silicone, polypropylene, PTFE, and phosphorylcholine coated poly(methylmethacrylate) were seeded with fibrinogen, fibrin, fibroblast, and macrophages and incubated for variable lengths of time. The quantification was performed using radioactivity, spectrophotometry, ATP dependent luminometry, and immunohistochemistry respectively.


RESULTS
Fibrinogen and fibrin adhesion to phosphorylcholine coated poly(methylmethacrylate) were significantly lower than PMMA (p=0.004). Phosphorylcholine coating of poly(methylmethacrylate) also significantly reduced the adhesion of human scleral fibroblast (p=0.002) and macrophage (p=0.01) compared with PMMA. All the other biomaterials showed either similar or insignificantly different levels of adhesion to all the proteins and cells tested compared with PMMA.


CONCLUSIONS
Phosphorylcholine coating is a new material technology that offers considerable promise in the field of glaucoma drainage device development.
Lim
K S
KS
Wound Healing Research Unit, Department of Pathology, Institute of Ophthalmology, London.
D000328:Adult; D001672:Biocompatible Materials / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D005337:Fibrin / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D020327:Glaucoma Drainage Implants; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D008422:Materials Testing; D010767:Phosphorylcholine; D019904:Polymethyl Methacrylate
D001672:Biocompatible Materials; D010767:Phosphorylcholine; D005337:Fibrin; D005340:Fibrinogen; D019904:Polymethyl Methacrylate