title: Development of murine leukemia virus-based self-activating vectors that efficiently delete the selectable drug resistance gene during reverse transcription.
abstract: Expression of the selectable drug resistance gene in retroviral vectors used for gene therapy can lead to a decreased expression of the gene of interest and may induce a host immune response, resulting in a decreased efficiency of gene therapy. In this study, we demonstrate that high-frequency deletion of direct repeats, an inherent property of reverse transcriptases, can be used to efficiently excise the drug resistance gene during reverse transcription. One retroviral vector containing a direct repeat deleted the neomycin resistance expression cassette during a single replication cycle at >99% efficiency.
authors.affiliation: Department of Genetics and Developmental Biology, West Virginia University, Morgantown, West Virginia 26506, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Delviks
authors.forename: V K
authors.initials: VK
authors.lastname: Pathak
mesh_terms: D000818:Animals; D002460:Cell Line; D004351:Drug Resistance / Q000235:genetics*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D009052:Leukemia Virus, Murine / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D014158:Transcription, Genetic
chemical_list: D012194:RNA-Directed DNA Polymerase