title: Expression of 15N-labeled eukaryotic cytochrome c in Escherichia coli.
abstract: We present a simple and inexpensive method for producing 15N-labeled Saccharomyces cerevisiae iso-1-cytochrome c in Escherichia coli. The labeled protein gives excellent NMR spectra.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill 27599, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Morar
authors.forename: D
authors.initials: D
authors.lastname: Kakouras
authors.forename: G B
authors.initials: GB
authors.lastname: Young
authors.forename: J
authors.initials: J
authors.lastname: Boyd
authors.forename: G J
authors.initials: GJ
authors.lastname: Pielak
mesh_terms: D003574:Cytochrome c Group / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D003574:Cytochrome c Group; D009587:Nitrogen Isotopes