Antiviral activity of enterocin CRL35 against herpesviruses.
Enterocin CRL35 is an antibacterial polypeptide of 3.5 x 10(3) Da produced by Enterococcus faecium CRL35. A series of experiments are described that show the enterocin also had antiviral activity against thymidine-kinase positive (tk+) and deficient (tk-) strains of herpes simplex (HSV) type 1 and 2 in Vero and BHK-21 cells. This activity was observed at 100 microg/ml, 15-fold lower than the cytotoxic concentration. In both cell lines there was a 2 log inhibition of infectivity. The compound inhibited viral multiplication in a dose-dependent manner and had no virucidal effect. Enterocin CRL35 also inhibited the virion-associated host shutoff in infected Vero cells showing that intracellular viral multiplication was affected.
Wachsman
M B
MB
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina. wachsman@qb.fcen.uba.ar
Farías
M E
ME
Takeda
E
E
Sesma
F
F
de Ruiz Holgado
A P
AP
de Torres
R A
RA
Coto
C E
CE
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000472:pathogenicity; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000472:pathogenicity; D014709:Vero Cells
D000998:Antiviral Agents; D001430:Bacteriocins; C121783:enterocin CRL35; D000212:Acyclovir