title: Correlated measurements of free and total intracellular calcium concentration in central nervous system neurons.
abstract: Transient changes in the intracellular concentration of free calcium ([Ca2+])i) act as a trigger or modulator for a large number of important neuronal processes. Such transients can originate from voltage- or ligand-gated fluxes of Ca2+ into the cytoplasm from the extracellular space, or by ligand- or Ca2+(-)gated release from intracellular stores. Characterizing the sources and spatio-temporal patterns of [Ca2+]i transients is critical for understanding the role of different neuronal compartments in dendritic integration and synaptic plasticity. Optical imaging of fluorescent indicators sensitive to free Ca2+ is especially suited to studying such phenomena because this approach offers simultaneous monitoring of large regions of the dendritic tree in individual living central nervous system neurons. In contrast, energy-dispersive X-ray (EDX) microanalysis provides quantitative information on the amount and location of intracellular total, i.e., free plus bound, calcium (Ca) within specific subcellular dendritic compartments as a function of the activity state of the neuron. When optical measurements of [Ca2+]i transients and parallel EDX measurements of Ca content are used in tandem, and correlated simultaneously with electrophysiological measurements of neuronal activity, the combined information provides a relatively general picture of spatio-temporal neuronal total Ca fluctuations. To illustrate the kinds of information available with this approach, we review here results from our ongoing work aimed at evaluating the role of various Ca uptake, release, sequestration, and extrusion mechanisms in the generation and termination of [Ca2+]i transients in dendrites of pyramidal neurons in hippocampal slices during and after synaptic activity. Our observations support the long-standing speculation that the dendritic endoplasmic reticulum acts not only as an intracellular Ca2+ source that can be mobilized by a signal cascade originating at activated synapses, but also as a major intracellular Ca sink involved in active clearance mechanisms after voltage- and ligand-gated Ca2+ influx.
authors.affiliation: Department of Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA. pozzomiller@nrc.uab.edu
authors.forename: L D
authors.initials: LD
authors.lastname: Pozzo-Miller
authors.forename: N B
authors.initials: NB
authors.lastname: Pivovarova
authors.forename: J A
authors.initials: JA
authors.lastname: Connor
authors.forename: T S
authors.initials: TS
authors.lastname: Reese
authors.forename: S B
authors.initials: SB
authors.lastname: Andrews
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000032:analysis*; D004577:Electron Probe Microanalysis; D005456:Fluorescent Dyes; D006801:Humans; D009474:Neurons / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium