[B cell acute lymphocytic leukemia with marked leukocytosis and t(3;15)(q27;q2?2)].
We report on a 16-year-old boy with B cell acute lymphocytic leukemia presenting marked leukocytosis (388,000/microliter) and resistance to multidrug chemotherapy. Karyotypical analysis revealed a novel t(3;15)(q27;q2?2) chromosomal abnormality. Because 3q27 is known to be a locus of the bcl-6 gene, which is frequently involved in B cell malignancies, molecular biological analyses were performed. Although no rearrangement was detected in 5 genes including bcl-6 on 3q27 and 2 genes on 15q2, reverse transcriptase-polymerase chain reaction procedures detected relatively strong mRNA expression of the bcl-6, smrp, dvl3, and tpml genes. These results indicate that immature leukemic cells with CD10 and CD34 positivity and rearrangement of the T cell receptor beta gene may coexist with relatively mature subpopulations that are positive for CD19 and CD20 surface markers, bcl-6 expression, and rearrangement of the gene for immunoglobulin kappa.
Yata
K
K
Department of Internal Medicine, Kawasaki Medical School.
Wada
H
H
Sugihara
T
T
Yamada
O
O
Otsuki
T
T
Nakazawa
N
N
Taniwaki
M
M
Akasaka
T
T
Ohno
H
H
Yawata
Y
Y
D000293:Adolescent; D002884:Chromosomes, Human, Pair 15; D002893:Chromosomes, Human, Pair 3; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D015448:Leukemia, B-Cell / Q000097:blood* / Q000235:genetics; D007964:Leukocytosis / Q000209:etiology*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors