A new peroxidase cDNA from white clover: its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae.
Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h. Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.
Crockard
M A
MA
Department of Applied Plant Science, Queen's University of Belfast, Northern Ireland. m.crockard@qub.ac.uk
Bjourson
A J
AJ
Cooper
J E
JE
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000235:genetics*; D018517:Plant Roots / Q000201:enzymology / Q000382:microbiology; D010946:Plants, Medicinal; D011549:Pseudomonas / Q000502:physiology; D012231:Rhizobium / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis
D018076:DNA, Complementary; D018744:DNA, Plant; D009195:Peroxidase