The broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311.
The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso) origins. pLF1311 belongs to the pE194 family of rolling circle-replicating plasmids. A derivative of pLF1311 that contains the cat gene of plasmid pC194 of Staphylococcus aureus and the oriT of RP4 was constructed and transferred by conjugative mobilization from Escherichia coli to various Gram-positive bacteria. The stable maintenance of this derivative was shown in some strains of Lactobacillus, Lactococcus, Enterococcus and Bacillus under non-selective conditions.
Aleshin
V V
VV
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), Moscow, Russia.
Semenova
E V
EV
Doroshenko
V G
VG
Jomantas
Y V
YV
Tarakanov
B V
BV
Livshits
V A
VA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D004247:DNA / Q000737:chemistry; D004265:DNA Helicases; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000737:chemistry; D004268:DNA-Binding Proteins; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000235:genetics; D016415:Sequence Alignment; D015534:Trans-Activators
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011506:Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004247:DNA; C091155:rapB protein, Bacillus subtilis; D010749:Phosphoprotein Phosphatases; D004265:DNA Helicases