[Expression of HBsAg by using various eukaryotic expression vectors].
OBJECTIVE
To establish efficient eukaryotic expression system of HBsAg and its variants.


METHODS
A series of expression vectors were constructed by cloning HBV S gene and its mutant genes from recombinant vector pBluescript pBKS(+)-HBs containing S gene and its mutant genes into eukaryotic expression vectors: pMEP4, pCEP4, pLXSN, PXT1, pcDNA3. These recombinant eukaryotic expression vectors were transfected into human hepatocellular carcinoma cells (HepG2).


RESULTS
The cell lines expressing recombinant proteins were stably established.


CONCLUSIONS
All expression vectors could express HBsAg and its variants, but their transfecting efficiency, expressing efficiency and stability of expression were different. The establishment of highly efficient expression system in this study paves the way for further study on biologic characters of HBsAg variants, genetic immunization and genetic therapy.
Gu
B
B
Chongqing Medical University.
Ren
H
H
D005057:Eukaryotic Cells / Q000378:metabolism*; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins