Two different forms of p53 localized differently within cells of urogenital tumours.
We analyzed the subcellular localization of p53 in prostate and bladder carcinoma cells. Using laser scanning microscopy and PAb1620, a monoclonal antibody recognizing the wildtype conformation of p53, and another monoclonal antibody directed against the mutant conformation of the protein (PAb240), we found two different subsets of p53 within the same cell. The wildtype subgroup was found in the nucleolus, whereas the mutant protein was confined to the nucleus. The results obtained by immunofluorescence were verified by Western blot analysis and immunoprecipitation. Thus, our findings demonstrate an unusual subcellular localization pattern of p53 in prostate and bladder cancer cells which may indicate another mechanism of inactivation of p53.
Benninghoff
J
J
Department of Urology, University of the Saarland, Homburg/Saar, Germany.
Kartarius
S
S
Teleb
Z
Z
Selter
H
H
Unteregger
G
G
Zwergel
T
T
Wullich
B
B
Montenarh
M
M
D015153:Blotting, Western; D002466:Cell Nucleolus / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D018613:Microscopy, Confocal; D009154:Mutation; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000235:genetics; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry*
D016159:Tumor Suppressor Protein p53