title: Distinct expression patterns of the p53-homologue p73 in malignant and normal hematopoiesis assessed by a novel real-time reverse transcription-polymerase chain reaction assay and protein analysis.
abstract: The role of the recently identified first p53-homologue, p73, in neoplastic transformation is unknown. To elucidate p73 gene expression in hematopoiesis, we investigated samples from chronic myeloid leukemia (CML) and acute myeloid leukemia patients, leukemia cell lines, as well as mature and immature normal hematopoietic cells by real-time quantitative RT-PCR and Western blot analysis. We found a distinct p73 expression profile with highest p73 mRNA transcript levels in hematopoietic malignancies such as CML blast crisis and acute myelogenous leukemia versus CML chronic phase and normal controls. Mono- and biallelic p73 expression was found in both normal and malignant hematopoiesis. p73 protein was expressed at various levels in leukemia samples and cell lines but could not be detected in any normal controls tested. Our results point to a distinct yet undefined role of p73 in the pathogenesis of myeloid neoplasms.
authors.affiliation: Department of Hematology/Oncology, Charité/Virchow-Clinic, Humboldt-University, Berlin, Germany.
authors.forename: U R
authors.initials: UR
authors.lastname: Peters
authors.forename: M P
authors.initials: MP
authors.lastname: Tschan
authors.forename: K A
authors.initials: KA
authors.lastname: Kreuzer
authors.forename: G
authors.initials: G
authors.lastname: Baskaynak
authors.forename: U
authors.initials: U
authors.lastname: Lass
authors.forename: A
authors.initials: A
authors.lastname: Tobler
authors.forename: M F
authors.initials: MF
authors.lastname: Fey
authors.forename: C A
authors.initials: CA
authors.lastname: Schmidt
mesh_terms: D000483:Alleles; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D016147:Genes, Tumor Suppressor; D006410:Hematopoiesis; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins