Gas chromatographic-mass spectrometric analysis of erythrocyte 3-deoxyglucosone in hemodialysis patients.
The erythrocyte levels of 3-deoxyglucosone (3-DG) were measured by a selected ion monitoring method of gas chromatography-chemical ionization mass spectrometry using [13C6]-3-DG as an internal standard. Because the erythrocyte levels of 3-DG measured after deproteinization using ethanol were 18 times higher than those using ultrafiltration, we used ethanol deproteinization for measurement of total 3-DG in the erythrocytes. The concentration of 3-DG was significantly elevated in hemodialysis (HD) patients compared with healthy subjects. Although HD treatment could remove the erythrocyte 3-DG efficiently, its post-HD levels were still elevated compared with the healthy subjects.
Tsukushi
S
S
Nagoya University, Daiko Medical Center, Japan.
Shimokata
K
K
Niwa
T
T
D000368:Aged; D016022:Case-Control Studies; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis
D003847:Deoxyglucose; C016350:3-deoxyglucosone