[The diagnosis of disorders of the T-cell immunity component in space flight].
The investigation yielded two modifications of the procedure FOR determination of T-lymphocytes' functional activity in microsamples of the finger capillary blood and PHA-assisted cultivation of cells in syringe for subsequent morphological analysis by cosmonauts in flight. Testing of the proposed modifications in 14 healthy donors with unconstrained work/rest schedule, 4 volunteers in 60- and 6 volunteers in 120-day HDT showed a rather high comparability of data obtained with the modified and genuine procedures for determination of the T-lymphocytes proliferative activity in cosmonauts before and after orbital flights. Thus, the modified procedures can be successfully used for monitoring the immune status of cosmonauts on missions, and individuals exposed to extreme environments.
Lesniak
A T
AT
Antropova
E N
EN
Meshkov
D O
DO
Rykova
M P
MP
D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002478:Cells, Cultured; D018475:Head-Down Tilt / Q000502:physiology; D006801:Humans; D007154:Immune System Diseases / Q000175:diagnosis* / Q000276:immunology; D007111:Immunity, Cellular; D008934:Mitogens; D010835:Phytohemagglutinins; D013026:Space Flight; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D013997:Time Factors
D008934:Mitogens; D010835:Phytohemagglutinins