Highly sensitive bioassay of lidocaine in human plasma by high-performance liquid chromatography-tandem mass spectrometry.
A very sensitive HPLC-tandem mass spectrometric (LC-MS-MS) method for assaying lidocaine in human plasma was set up and fully validated. Lidocaine and an internal standard (bupivacaine) were extracted from 1 ml of alkalinized plasma with tert.-butylmethyl ether, back-extracted to a H3PO4 acidified solution and injected into a C18 column. Acetonitrile-26 mmol/l ammonium acetate, pH 4.5 (70:30, v/v) was the mobile phase at a flow-rate of 1 ml/min. The effluent was detected by PE Sciex API 365 LC-MS-MS system in positive ion mode. Ionisation was performed using an atmospheric pressure chemical ionization ion source operating at 400 degrees C. The multi reaction monitoring transition 235-->86 was monitored. Linearity was ascertained in the 0.2-30 ng/ml range with a limit of quantitation of 0.2 ng/ml. Intra- and inter-assay precision and accuracy were < or =3.8%. The high sensitivity and specificity achieved by the method allowed concentrations of lidocaine to be measured in plasma of healthy subjects topically treated with lidocaine (5% ointment) on normal skin over a 32-h period after dosing.
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I.P.A.S. S.A., Ligornetto, Switzerland.
Mazzucchelli
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P
Marzo
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D000287:Administration, Topical; D000779:Anesthetics, Local / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D001681:Biological Assay; D001682:Biological Availability; D002138:Calibration; D005260:Female; D006801:Humans; D008012:Lidocaine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000779:Anesthetics, Local; D008012:Lidocaine