Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus.
A monoclonal antibody (MAb) blocking ELISA (Blck-ELISA) was developed to detect antibodies against Maedi-Visna virus (MVV) in sheep sera. The assay employs a MAb directed against the envelope protein p90 of the virus in a sandwich blocking procedure. To determine whether the MAb was a potential antibody for developing a Blck-ELISA, a collection of three hundred sera obtained from several sheep flocks known to be infected with MVV were used to examine the sensitivity of the Blck-ELISA. A total of 50 serum samples originating from a flock free of MVV were tested to assess the specificity of the assay. The results were compared with a commercial indirect ELISA (I-ELISA) and samples giving a conflicting or doubtful result were tested by immunoblot. The Blck-ELISA proved to be specific, sensitive and it showed high reproducibility and low variability.
Fevereiro
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M
Laboratório Nacional de Investigação Veterinária, Dept. Virologia, Lisbon, Portugal. vir.Iniv@mail.telepac.pt
Barros
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Fagulha
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D000818:Animals; D019138:Antibodies, Blocking / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000032:analysis*; D002478:Cells, Cultured; D002831:Choroid Plexus; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D012756:Sheep; D014759:Viral Envelope Proteins / Q000276:immunology; D014790:Visna-maedi virus / Q000276:immunology*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins