title: Activin incorporation into vitellogenic oocytes of Xenopus laevis.
abstract: Activin uptake into Xenopus oocytes was studied by several complementary methods. Immunocytochemistry of adult ovary localized activin and follistatin in the cytoplasm of vitellogenic oocytes and surrounding follicle cells. Surface plasmon resonance analysis of protein interaction kinetics indicated that while follistatin or a complex of activin-follistatin bound to yolk vitellogenin, activin alone did not. Radioactive tracer analysis measured specific incorporation of activin by viable oocytes in vitro. Together, the results suggest that vitellogenic oocytes can import activins from follicle cells and that follistatin may act as a chaperone for binding activin to vitellogenin in yolk platelets.
authors.affiliation: Department of Life Sciences (Biology), Tokyo University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Fukui
authors.forename: R
authors.initials: R
authors.lastname: Shiurba
authors.forename: M
authors.initials: M
authors.lastname: Asashima
mesh_terms: D028341:Activins; D000818:Animals; D001692:Biological Transport; D005260:Female; D038681:Follistatin; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry; D007265:Inhibins / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D020349:Surface Plasmon Resonance; D014819:Vitellogenins / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D038681:Follistatin; D006023:Glycoproteins; D014819:Vitellogenins; D028341:Activins; D007265:Inhibins