In vivo modulation of the acidic N-glycans from rat liver dipeptidyl peptidase IV by N-propanoyl-D-mannosamine.
Derivatives of N-acyl-D-mannosamine differing in the N-acyl-side chain can be metabolically converted into neuraminic acids with corresponding N-acyl side chains. In the present study we show the in vivo modulation of sialic acids in membrane-bound dipeptidyl peptidase IV (CD 26) from rat liver after administration of N-propanoyl-D-mannosamine. Treatment of rats with this unphysiological precursor resulted in an incorporation of N-propanoylneuraminic acid into N-linked glycans of dipeptidyl peptidase IV.
Stehling
P
P
Institut für Molekularbiologie and Biochemie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Arnimallee 22, Berlin-Dahlem, D-14195, Germany.
Grams
S
S
Nuck
R
R
Grunow
D
D
Reutter
W
W
Gohlke
M
M
D000818:Animals; D002852:Chromatography, Ion Exchange; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D006031:Glycosylation; D006595:Hexosamines / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006595:Hexosamines; D037102:Lectins; D011134:Polysaccharides; D012794:Sialic Acids; C076271:N-propanoylmannosamine; D018819:Dipeptidyl Peptidase 4