Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators.
SWI/SNF is a chromatin remodeling complex that facilitates expression of a number of yeast genes. Here we demonstrate that SWI/SNF can be recruited from yeast nuclear extracts by a transcriptional activator. Recruitment is dependent on an activation domain but not on promoter sequences, TBP, or RNA polymerase II holoenzyme. We also show that acidic activation domains can target SWI/SNF remodeling activity. These results demonstrate that SWI/SNF activity can be targeted by gene-specific activators and that this recruitment can occur independently of Pol II holoenzyme.
Yudkovsky
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Molecular and Cellular Biology Program, University of Washington, The Fred Hutchinson Cancer Research Center, Seattle Washington 98109 USA.
Logie
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Hahn
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Peterson
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CL
D002843:Chromatin / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D020035:Holoenzymes / Q000378:metabolism; D008957:Models, Genetic; D009707:Nucleosomes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013997:Time Factors; D015533:Transcriptional Activation
D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D020035:Holoenzymes; D009707:Nucleosomes; D012319:RNA Polymerase II