The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans.
Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transformed to the acu+ phenotype with a clone of ACS (facA) from Aspergillus nidulans. The acuB mutant was transformed with the A. niger facB clone which has been identified by cross-hybridisation of an A. nidulans facB clone. These results confirm that acuA in A. niger is the gene for ACS and acuB is analogous to the A. nidulans facB regulatory gene.
Papadopoulou
S
S
Department of Biological Sciences, University of Hull, UK.
Sealy-Lewis
H M
HM
D000106:Acetate-CoA Ligase / Q000235:genetics; D000123:Acetyltransferases; D001233:Aspergillus nidulans / Q000235:genetics*; D001234:Aspergillus niger / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D005656:Fungal Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D005809:Genes, Regulator; D015534:Trans-Activators / Q000235:genetics; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D005656:Fungal Proteins; D015534:Trans-Activators; C089828:acuB protein, Bacillus subtilis; D000123:Acetyltransferases; C089827:acuA protein, Bacillus subtilis; D000106:Acetate-CoA Ligase