title: A beta-1,4-endoglucanase-encoding gene from Cellulomonas pachnodae.
abstract: A gene library of Cellulomonas pachnodae was constructed in Escherichia coli and was screened for endoglucanase activity. Five endoglucanase-positive clones were isolated that carried identical DNA fragments. The gene, designated cel6A, encoding an endoglucanase enzyme, belongs to the glycosyl hydrolase family 6 (cellulase family B). The recombinant Cel6A had a molecular mass of 53 kDa, a pH optimum of 5.5, and a temperature optimum of 50-55 degrees C. The recombinant endoglucanase Cel6A bound to crystalline cellulose and beech litter. Based on amino acid sequence similarity, a clear cellulose-binding domain was not distinguished. However, the regions in the Cel6A amino acid sequence at the positions 262-319 and 448-473, which did not show similarity to any of the known family-6 glycosyl hydrolases, may be involved in substrate binding.
authors.affiliation: Department of Microbiology and Evolutionary Biology, Faculty of Science, University of Nijmegen, The Netherlands.
authors.forename: A E
authors.initials: AE
authors.lastname: Cazemier
authors.forename: J C
authors.initials: JC
authors.lastname: Verdoes
authors.forename: H J
authors.initials: HJ
authors.lastname: Op den Camp
authors.forename: J H
authors.initials: JH
authors.lastname: Hackstein
authors.forename: A J
authors.initials: AJ
authors.lastname: van Ooyen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004064:Digestive System / Q000382:microbiology; D005798:Genes, Bacterial; D016988:Gram-Positive Asporogenous Rods, Irregular / Q000201:enzymology / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D007313:Insecta / Q000382:microbiology; D008969:Molecular Sequence Data; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D011994:Recombinant Proteins; D002480:Cellulase