The use of differential scanning calorimetry (DSC) to determine the correctness of folding of cloned proteins.
The advantages and limitations of use of the differential scanning calorimetry technique to determine whether cloned and expressed mutant proteins are folded correctly are explained and discussed. Examination of stabilization of these proteins to thermal denaturation by specific ligand binding can provide the required information.
Brewer
J M
JM
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
D002152:Calorimetry, Differential Scanning / Q000379:methods*; D006639:Histidine; D006358:Hot Temperature; D009154:Mutation; D010751:Phosphopyruvate Hydratase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D017186:Titrimetry
D011994:Recombinant Proteins; D006639:Histidine; D010751:Phosphopyruvate Hydratase