title: Syndecan-4 core protein is sufficient for the assembly of focal adhesions and actin stress fibers.
abstract: The formation of focal adhesions and actin stress fibers on fibronectin is dependent on signaling through (&bgr;)1 integrins and the heparan sulfate proteoglycan syndecan-4, and we have analyzed the requirement of the glycosaminoglycan chains of syndecan-4 during these events. Chinese hamster ovary cells with mutations in key enzymes of the glycanation process do not synthesize glycosaminoglycan chains and are unable to assemble actin stress fibers and focal contacts when cultured on fibronectin. Transfection of the mutant cells with a cDNA that encodes the core protein of chicken syndecan-4 leads to the production of unglycanated core protein. The overexpression of syndecan-4 core protein in these mutant cells increases cell spreading and is sufficient for these cells to assemble actin stress fibers and focal adhesions similar to wild-type cells seeded on fibronectin and vitronectin matrices. Syndecan-4 core protein colocalizes to focal contacts in mutant cells that have been transfected with the syndecan-4 core protein cDNA. These data indicate an essential role for the core protein of syndecan-4 in the generation of signals leading to actin stress fiber and focal contact assembly.
authors.affiliation: Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Building 149, Charlestown, Massachusetts 02129, USA. paul.goetinck@cbrc2.mgh.harvard.edu
authors.forename: F
authors.initials: F
authors.lastname: Echtermeyer
authors.forename: P C
authors.initials: PC
authors.lastname: Baciu
authors.forename: S
authors.initials: S
authors.lastname: Saoncella
authors.forename: Y
authors.initials: Y
authors.lastname: Ge
authors.forename: P F
authors.initials: PF
authors.lastname: Goetinck
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology*; D002645:Chickens; D006224:Cricetinae; D006025:Glycosaminoglycans / Q000502:physiology; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D011509:Proteoglycans / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D053671:Syndecan-4; D014162:Transfection; D016596:Vinculin / Q000502:physiology
chemical_list: D000199:Actins; D006025:Glycosaminoglycans; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D011994:Recombinant Proteins; D053671:Syndecan-4; D016596:Vinculin