A method for quick measurement of protein binding to unilamellar vesicles.
A general method for measuring interaction of liposome-protein (or potentially small molecules) was developed. This method utilizes biotinylated liposomes to incubate with interactants. Streptavidin-coated paramagnetic resins were then added and the liposomes (along with bound materials) can be quickly separated under a magnetic field or by low speed centrifugation. Subsequently, concentration of unbound materials (in the supernatants) can be directly determined. The described method is particularly useful for proteins or compounds that are not very soluble under certain assay conditions.
Zhan
H
H
Axys Pharmaceuticals, Inc., South San Francisco, CA 94080, USA. hangjun_zhan@axyspharm.com
D000818:Animals; D019921:Biotinylation / Q000379:methods; D002498:Centrifugation; D005170:Factor X / Q000737:chemistry*; D005456:Fluorescent Dyes; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008280:Magnetics; D008863:Microspheres; D010743:Phospholipids / Q000737:chemistry; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry
D005456:Fluorescent Dyes; D008081:Liposomes; D010743:Phospholipids; D005170:Factor X; D019809:Streptavidin