Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin.
The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (approximately 95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST.
Udupi
V
V
Department of Surgery, The University of Texas Medical Branch, Galveston 77555-0725, USA.
Lee
H M
HM
Kurosky
A
A
Greeley
G H
GH
D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000502:physiology*; D002276:Carcinoid Tumor / Q000201:enzymology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism*; D006801:Humans; D010187:Pancreatic Hormones / Q000096:biosynthesis / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000378:metabolism; D043484:Proprotein Convertases; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D010187:Pancreatic Hormones; D012333:RNA, Messenger; C050836:pancreastatin; D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases