Lactulose feeding in piglets: a model for persistent diarrhea and colitis induced by severe sugar malabsorption.
We sought to determine, in a piglet model, whether severe sugar malabsorption causes colonic injury or inflammation. Twenty-four piglets were randomized to receive either control formula (CON) or CON supplemented with lactulose (LAC) (N = 12 each group). After seven days, inflammation, apoptosis, and crypt cell proliferation were assessed in the proximal colon (cecum). Lactulose feeding caused persistent diarrhea. In both groups, breath H2 concentration was low, suggesting no increased fermentation in the LAC group. Weight gain/volume formula intake was identical in the CON and LAC groups (0.09+/-0.13 and 0.09+/-0.11 g/ml) respectively. Injury to the colon did not occur, but inflammation of the colon (scale 0-5) was greater in LAC (score of 1.5+/-1.38) than in CON (0.42+/-0.79; P<0.05). Cell proliferation at the basal 40% of the crypt was 92% increased in CON (labeling index 22.8+/-9.9 vs. 11.9+/-2.8; P<0.05). We conclude that persistent feeding during severe sugar malabsorption permits weight gain but may cause colitis.
Kien
C L
CL
Children's Hospital Research Foundation and the Department of Pediatrics, The Ohio State University, Columbus, USA.
Murray
R D
RD
Qualman
S J
SJ
Marcon
M
M
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D050260:Carbohydrate Metabolism; D002455:Cell Division / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology; D003967:Diarrhea / Q000139:chemically induced* / Q000473:pathology; D004195:Disease Models, Animal; D005285:Fermentation; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D007792:Lactulose / Q000633:toxicity*; D008286:Malabsorption Syndromes / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D013552:Swine
D007792:Lactulose