Cloning, sequence analysis, and chromosomal localization of the novel human integrin alpha11 subunit (ITGA11).
The integrins are a large family of cell adhesion molecules consisting of noncovalently associated alphabeta heterodimers. We have cloned and sequenced the cDNA of a novel human integrin alpha-subunit, designated alpha11. The alpha11 cDNA encodes a mature protein with a large 1120-residue extracellular domain that contains an I-domain of 207 residues and is linked by a transmembrane domain to a short cytoplasmic domain of 24 amino acids. The deduced alpha11 protein shows the typical structural features of integrin alpha-subunits and is similar to a distinct group of alpha-subunits from collagen-binding integrins. However, it differs from most integrin alpha-chains by an incompletely preserved cytoplasmic GFFKR motif. The human ITGA11 gene was localized to bands q22.3-q23 on chromosome 15, and its transcripts were found in a variety of tissues, but predominantly in bone, cartilage, cardiac muscle, and skeletal muscle. Expression of a 5.5-kb alpha11 mRNA was detectable in small intestine.
Lehnert
K
K
Faculty of Medicine and Health Science, University of Auckland, Auckland, New Zealand.
Ni
J
J
Leung
E
E
Gough
S M
SM
Weaver
A
A
Yao
W P
WP
Liu
D
D
Wang
S X
SX
Morris
C M
CM
Krissansen
G W
GW
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D039001:Integrin alpha Chains; D016023:Integrins / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D038981:Receptors, Collagen; D014018:Tissue Distribution
D017931:DNA Primers; D018076:DNA, Complementary; C121535:ITGA11 protein, human; D039001:Integrin alpha Chains; D016023:Integrins; D012333:RNA, Messenger; D038981:Receptors, Collagen