Increased susceptibility to carcinogen-induced mammary tumors in MMTV-Cdc25B transgenic mice.
Cdc25 phosphatases activate cyclin-dependent kinases (Cdks) by dephosphorylating critical phospho-tyrosine and phospho-threonine residues on these proteins. Several types of studies indicate that Cdc25s can enhance cell proliferation and oncogenesis. Furthermore, overexpression of Cdc25A and/or B have been detected in several types of primary human cancers, including breast cancers. To further assess the oncogenic capacity of Cdc25B in vivo, we have generated transgenic mice that overexpress Cdc25B in the mammary epithelium, driven by the MMTV - LTR promoter. Although these mice are grossly normal for up to 18 months, the ectopic expression of Cdc25B in their mammary glands increases the susceptibility of these mice to induction of mammary tumors by the carcinogen 9,10-dimethyl-1, 2-benzanthracene (DMBA).
Yao
Y
Y
Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA.
Slosberg
E D
ED
Wang
L
L
Hibshoosh
H
H
Zhang
Y J
YJ
Xing
W Q
WQ
Santella
R M
RM
Weinstein
I B
IB
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D004847:Epithelial Cells / Q000201:enzymology; D015967:Gene Expression Regulation, Viral; D020022:Genetic Predisposition to Disease; D007527:Isoenzymes / Q000235:genetics*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology / Q000235:genetics; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D020079:Terminal Repeat Sequences; D019076:Transgenes; D020687:cdc25 Phosphatases
D002273:Carcinogens; D018797:Cell Cycle Proteins; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene; D010749:Phosphoprotein Phosphatases; D020687:cdc25 Phosphatases