title: Effects of thiopental, ketamine, etomidate, propofol and midazolam on the production of adrenomedullin and endothelin-1 in vascular smooth muscle cells.
abstract: Endothelin-1 (ET-1) and adrenomedullin (AM) are potent vasoconstrictive and vasodilative peptides. Vascular smooth muscle cells are one of the major producing tissues of these peptides. We investigated effect of thiopental, ketamine, etomidate, midazolam and propofol on the production of ET-1 and AM in cultured rat aorta vascular smooth muscle cells. Rat cultured vascular smooth muscle cells are used and the dose-dependent effect (10(-8)-10(-4) M) of thiopental, ketamine, etomidate, midazolam, and propofol on the production of ET-1 and AM was examined. The concentration of these peptides in the culture medium were measured by radioimmunoassay. Ketamine, etomidate, propofol and midazolam, but not thiopental, decreased the production of ET-1 at a concentration of 10(-4) M and the effect of midazolam is the most potent. Thiopental and etomidate produced small but significant increases in the production of AM at a concentration of 10(-4) M. Ketamine did not affect the production, but propofol and midazolam decreased the production at 10(-4) M and 10(-5) M, respectively. These results suggest that thiopental, ketamine, etomidate, propofol and midazolam differentially affect the production of ET-1 and AM in rat vascular smooth muscle cells.
authors.affiliation: Department of Anesthesiology, National Cardiovascular Center, Suita, Osaka, Japan. yhayashi@anes.med.osaka-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Minamino
authors.forename: Y
authors.initials: Y
authors.lastname: Isumi
authors.forename: K
authors.initials: K
authors.lastname: Kangawa
authors.forename: M
authors.initials: M
authors.lastname: Kuro
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
mesh_terms: D053607:Adrenomedullin; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000096:biosynthesis*; D005045:Etomidate / Q000494:pharmacology; D007649:Ketamine / Q000494:pharmacology; D008874:Midazolam / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D015742:Propofol / Q000494:pharmacology; D051381:Rats; D013874:Thiopental / Q000494:pharmacology; D014665:Vasodilator Agents / Q000378:metabolism*
chemical_list: D018686:Anesthetics, Intravenous; D019332:Endothelin-1; D010455:Peptides; D014665:Vasodilator Agents; D053607:Adrenomedullin; D007649:Ketamine; D013874:Thiopental; D008874:Midazolam; D015742:Propofol; D005045:Etomidate