Capillary electrophoresis of double-stranded DNA in an untreated capillary.
Using the zwitterionic buffer N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) in the presence of a high-molecular-mass hydroxypropylmethylcellulose (HPMC) as a sieving polymer and ethidium bromide double-stranded DNA (dsDNA) was separated in an untreated capillary. The HEPES buffer shielded the DNA against the capillary wall interaction and decreased the electroosmotic flow enabling a good separation of the DNA similar to that obtained in a commercially coated capillary. In addition to the low cost of the untreated capillary it can be washed after each run. Furthermore, stacking with hydrodynamic injection filling about half of the capillary volume is demonstrated.
Shihabi
Z K
ZK
Pathology Department, Wake Forest University Baptist Medical Center, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
D002021:Buffers; D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007267:Injections / Q000379:methods
D002021:Buffers; D004247:DNA