Induction of apoptosis in Herpesvirus saimiri-immortalized T lymphocytes by blocking interaction of CD28 with CD80/CD86.
We have previously shown that Herpesvirus saimiri (HVS) immortalizes primary macaque monkey T lymphocytes. In this study, we examined the characteristics of the immortalized T cells. The cells showed the phenotype of activated T lymphoblasts (CD3(+) CD25(+) CD69(+) MHC-IIDR(+)) and produced no infectious virus while viral DNA was detected in the Hirt DNA. Interestingly, both a major costimulatory molecule, CD28, and its ligands, CD80/CD86, were coexpressed on the immortalized T cells. The treatment of the cells with a neutralizing monoclonal antibody against CD28, which blocks interaction of CD28 with CD80/CD86, resulted in retarded cell growth and in induction of apoptosis. The effect of the antibody treatment was not overcome by exogenous interleukin-2 treatment. These findings demonstrate the requirement of interaction of CD28 with CD80/CD86 for the optimal growth of HVS-immortalized T cells.
Akari
H
H
School of Medicine, University of Tokushima, 3 Kuramoto, Tokushima, 770-8503, Japan.
Fukumori
T
T
Iida
S
S
Adachi
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism*; D017209:Apoptosis; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D006567:Herpesvirus 2, Saimiriine; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008252:Macaca fascicularis; D008562:Membrane Glycoproteins / Q000378:metabolism; D011485:Protein Binding; D013601:T-Lymphocytes / Q000821:virology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D018106:CD28 Antigens; D007376:Interleukin-2; D008562:Membrane Glycoproteins