Neural invasion of two virulent suid herpesvirus 1 strains in neonatal pigs with or without maternal immunity.
The neural invasion of two virulent Suid Herpesvirus 1 (SHV1) strains was examined in neonatal pigs with or without maternal immunity. One-week-old pigs with comparable levels of maternal immunity (SN-titer = 12-48) were intranasally inoculated with 10(7.0) TCID50 of either of the Ka or E21 strains. The invasion of the strains was examined in the nasal mucosa and in three neuronal levels of the trigeminal nervous pathway as well as in three levels of the olfactory nervous pathway by virus titration and immunohistochemistry (IHC). In control pigs without specific antibodies, both strains invaded up to the end level of each neural pathway. In pigs with maternal immunity, the Ka strain invaded only up to the 2nd level of each pathway with titers being significantly lower (p<0.05) than in the negative controls. However, the E21 strain invaded up to the end levels in both neural pathways of immune pigs with virus titers being similar to those observed in non-immune pigs (p>0.05). IHC revealed that maternal antibodies can protect against a fibroblast-mediated spread of the Ka strain in the lamina propria of the nasal mucosa, as well as against a local spread of the Ka and E21 strains from neurons to their satellite cells in the trigeminal ganglion. In conclusion, the nature of virus strain determines the invasion of SHV1 within the nervous system of maternally-immune neonatal pigs.
Kritas
S K
SK
Clinic of Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece. skritas@vet.uth.gr
Pensaert
M B
MB
Nauwynck
H J
HJ
Kyriakis
S C
SC
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D003588:Cytopathogenic Effect, Viral; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000472:pathogenicity; D007112:Immunity, Maternally-Acquired / Q000276:immunology*; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000821:virology; D009474:Neurons / Q000276:immunology / Q000473:pathology / Q000821:virology; D009500:Neutralization Tests / Q000662:veterinary; D009833:Olfactory Pathways / Q000276:immunology / Q000473:pathology / Q000821:virology; D010523:Peripheral Nervous System Diseases / Q000276:immunology / Q000662:veterinary*; D011557:Pseudorabies / Q000276:immunology*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D012668:Trigeminal Ganglion / Q000276:immunology / Q000473:pathology / Q000821:virology; D014774:Virulence
D000914:Antibodies, Viral