title: Preparation of specific antisera to 15alpha-hydroxyestrogens.
abstract: The synthesis of haptens of 15alpha-hydroxyestrone, 15alpha-hydroxyestradiol, and 15alpha-hydroxyestriol (estetrol) was undertaken, to obtain specific antisera required for enzyme immunoassay. 3-(1-Carboxypropyl) ethers of these 15alpha-hydroxyestrogens were prepared and conjugated with bovine serum albumin and horseradish peroxidase. The specificity of antisera elicited against bovine serum albumin conjugates was checked by the enzyme immunoassay by using horseradish peroxidase-labeled antigen, and proved to be satisfactory in terms of cross-reactivities to related compounds.
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Kanagawa, Japan. e-suzuki@ina-research.co.jp
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Nakagomi
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Agui
authors.forename: S
authors.initials: S
authors.lastname: Iida
authors.forename: K
authors.initials: K
authors.lastname: Konno
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: H
authors.initials: H
authors.lastname: Kurihara
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuki
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: H
authors.initials: H
authors.lastname: Ono
mesh_terms: D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004958:Estradiol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D004970:Estrone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D006894:Hydroxyestrones / Q000138:chemical synthesis* / Q000276:immunology*; D007106:Immune Sera / Q000737:chemistry* / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013388:Succinimides / Q000737:chemistry
chemical_list: C122179:15-hydroxyestrone; D006894:Hydroxyestrones; D007106:Immune Sera; D013388:Succinimides; D012710:Serum Albumin, Bovine; D004970:Estrone; D004958:Estradiol; C011856:15 alpha-hydroxyestradiol; C001426:N-hydroxysuccinimide