Transient expression of the beta-glucuronidase gene in tissues of Arabidopsis thaliana by bombardment-mediated transformation.
Particle bombardment has proved to be useful for the transformation of plants. We have previously reported successful transient expression of the beta-glucuronidase (GUS) gene in cultured plant cells and tissues and the stable transformation of various plants using a pneumatic particle gun. In this chapter, we describe transient expression of the GUS gene in Arabidopsis thaliana leaves and roots using the pneumatic particle gun.
Seki
M
M
Laboratory of Plant Molecular Biology, Instutuite of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, Ibraki, Japan. mseki@rtc.riken.go.jp
Iida
A
A
Morikawa
H
H
D017360:Arabidopsis / Q000235:genetics*; D019470:Biolistics; D005966:Glucuronidase / Q000235:genetics*; D014170:Transformation, Genetic
D005966:Glucuronidase