title: Automation of functional assays by flow injection fluorescence microscopy.
abstract: Bead-injection spectroscopy is a novel technique that uses immobilized eukaryotic cells on microbeads as a renewable biosensor for fluorescence microscopy. The use of a flow injection instrument allows fast functional assays that generate full kinetic characterization of a drug. Because the cell population is automatically replaced for each assay, variability is minimized, thus allowing greater accuracy.
authors.affiliation: Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA. scamp@u.washington.edu
authors.forename: L D
authors.initials: LD
authors.lastname: Scampavia
authors.forename: P S
authors.initials: PS
authors.lastname: Hodder
authors.forename: I
authors.initials: I
authors.lastname: Lähdesmäki
authors.forename: J
authors.initials: J
authors.lastname: Ruzicka
mesh_terms: D001709:Biotechnology / Q000639:trends*; D018914:Cells, Immobilized; D005434:Flow Cytometry / Q000379:methods* / Q000639:trends*; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000639:trends*; D008863:Microspheres