Genetic analysis of rolled, which encodes a Drosophila mitogen-activated protein kinase.
Genetic and molecular characterization of the dominant suppressors of D-raf(C110) on the second chromosome identified two gain-of-function alleles of rolled (rl), which encodes a mitogen-activated protein (MAP) kinase in Drosophila. One of the alleles, rl(Su23), was found to bear the same molecular lesion as rl(Sem), which has been reported to be dominant female sterile. However, rl(Su23) and the current stock of rl(Sem) showed only a weak dominant female sterility. Detailed analyses of the rl mutations demonstrated moderate dominant activities of these alleles in the Torso (Tor) signaling pathway, which explains the weak dominant female sterility observed in this study. The dominant rl mutations failed to suppress the terminal class maternal-effect mutations, suggesting that activation of Rl is essential, but not sufficient, for Tor signaling. Involvement of rl in cell proliferation was also demonstrated by clonal analysis. Branching and integration of signals in the MAP kinase cascade is discussed.
Lim
Y M
YM
Laboratory of Developmental Biology, Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
Nishizawa
K
K
Nishi
Y
Y
Tsuda
L
L
Inoue
Y H
YH
Nishida
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D048049:Extracellular Signal-Regulated MAP Kinases; D005123:Eye / Q000648:ultrastructure; D005260:Female; D005799:Genes, Dominant; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007247:Infertility, Female / Q000235:genetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013489:Suppression, Genetic
D029721:Drosophila Proteins; D019908:Proto-Oncogene Proteins c-raf; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases; C086795:rl protein, Drosophila