title: Metal binding to the HIV nucleocapsid peptide.
abstract: Co(II) and Zn(II) binding constants have been measured for binding to the HIV-1 nucleocapsid N-terminal metal binding domain (residues 1-18), using competition titration methods and monitoring Co(II) binding by visible absorbance spectroscopy. Enthalpies for binding were directly measured by isothermal titration colorimetry. The results are compared with recent studies of related systems, including a study of Zn(II) binding by the full length protein.
authors.affiliation: Department of Chemistry, Princeton University, NJ 08544, USA.
authors.forename: G
authors.initials: G
authors.lastname: McLendon
authors.forename: H
authors.initials: H
authors.lastname: Hull
authors.forename: K
authors.initials: K
authors.lastname: Larkin
authors.forename: W
authors.initials: W
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins; D002614:Chelating Agents / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism*; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D015683:Gene Products, gag / Q000378:metabolism*; D015497:HIV-1; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017510:Protein Folding; D013054:Spectrophotometry, Atomic; D013816:Thermodynamics; D014764:Viral Proteins; D015032:Zinc / Q000378:metabolism*; D054301:gag Gene Products, Human Immunodeficiency Virus
chemical_list: D036022:Capsid Proteins; D002614:Chelating Agents; D015683:Gene Products, gag; C071884:NCP7 protein, Human immunodeficiency virus 1; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; D003035:Cobalt; D004492:Edetic Acid; D015032:Zinc; C026060:N-(hydroxyethyl)ethylenediaminetriacetic acid