Small zone, high-speed gel filtration chromatography to detect protein aggregation associated with light chain pathologies.
Small zone gel filtration chromatography can be used for qualitative and quantitative analysis of protein interactions and aggregation phenomena. The technique is fast, accessible to most laboratories, and can be combined with computer simulation to extract quantitative information from experimental data. The programs KRUNCH and SCIMZ will be furnished on written request to the authors.
Raffen
R
R
Biosciences Division, Argonne National Laboratory, Illinois 60439, USA.
Stevens
F J
FJ
D001536:Bence Jones Protein / Q000737:chemistry; D002850:Chromatography, Gel / Q000379:methods*; D003198:Computer Simulation; D019281:Dimerization; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000737:chemistry*; D017510:Protein Folding
D007147:Immunoglobulin Light Chains; D001536:Bence Jones Protein