title: Construction and evaluation of a delta cya delta crp Salmonella typhimurium strain expressing avian pathogenic Escherichia coli O78 LPS as a vaccine to prevent airsacculitis in chickens.
abstract: Avian pathogenic strains of Escherichia coli cause a number of extraintestinal diseases in poultry, including airsacculitis and colisepticemia. Expression of O78 lipopolysaccharide (LPS) is frequently associated with pathogenic isolates. Salmonella, a common poultry contaminant, is a major public health concern. The purpose of this work was to develop an E. coli vaccine for poultry with the use of an attenuated Salmonella typhimurium carrier that would benefit both the bird and the consumer. Orally administered attenuated S. typhimurium delta cya delta crp strains have been shown to provide excellent protection against wild-type Salmonella challenge in chickens. This work describes the construction of a delta cya delta crp derivative of an avian pathogenic S. typhimurium that expresses both the homologous group B determinants (O1,4,5,12) and the heterologous E. coli O78 LPS O antigens. This was accomplished by inserting the E. coli rfb region, which encodes the genes required for O78 expression, into the chromosomal cya gene of S. typhimurium, creating a defined deletion/insertion mutation. A delta crp mutation was introduced in a subsequent step. Expression of both O antigens was stable in vitro and in vivo. Vaccination of white leghorn chicks at day of hatch and 14 days with the recombinant vaccine strain induced serum immune responses against both S. typhimurium and E. coli LPS and protected the birds against subsequent challenge with an avian pathogenic E. coli O78 strain. Introduction of a mutation in rfc, which encodes the O antigen polymerase, reduced the chain length of the S. typhimurium LPS without affecting the expression of O78. The rfc mutation further enhanced the ability of the vaccine strain to protect chickens against E. coli challenge.
authors.affiliation: Megan Health, Inc., St. Louis, MO 63110, USA.
authors.forename: K
authors.initials: K
authors.lastname: Roland
authors.forename: R
authors.initials: R
authors.lastname: Curtiss
authors.forename: D
authors.initials: D
authors.lastname: Sizemore
mesh_terms: D000400:Air Sacs; D000818:Animals; D016470:Bacteremia / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D001428:Bacterial Vaccines; D002645:Chickens; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D008070:Lipopolysaccharides / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D012141:Respiratory Tract Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic
chemical_list: D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic