A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain).
A reverse transcription-polymerase chain reaction assay was developed for the detection of avian pneumovirus (Colorado strain) (APV-Col). The specific primers were designed from the published sequence of the matrix protein gene of APV-Col. The primers amplified a product of 631 nucleotides from APV-Col. The assay identified only APV-Col and did not react with Newcastle disease virus and infectious bronchitis virus.
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Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Reynolds
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D000818:Animals; D002642:Chick Embryo; D017931:DNA Primers; D001351:Infectious bronchitis virus / Q000235:genetics / Q000302:isolation & purification; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification; D018111:Pneumovirus / Q000235:genetics / Q000302:isolation & purification*; D018186:Pneumovirus Infections / Q000175:diagnosis / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012680:Sensitivity and Specificity; D014422:Turkeys
D017931:DNA Primers; D012367:RNA, Viral