title: Human peripheral blood contains two distinct lineages of dendritic cells.
abstract: Human peripheral blood contains two populations of dendritic cells (DC) but their developmental relationship has not been established. Freshly isolated CD11c- DC possessed a lymphoid morphology, lacked myeloid markers but expressed lymphoid markers (CD4+ CD10+) whilst the CD11c+ DC were monocytoid in appearance and expressed myeloid markers. Although both populations were allostimulatory, only the CD11c+ DC were able to take up antigen. Irrespective of the culture conditions the CD11c- cells developed into CD11c- CD13- CD33- CD4+ CD1a- CD83+/- DC. In contrast, cultured CD11c+ cells developed the phenotype CD11c+ CD13+ CD33+/- CD4- CD1a+ CD83+ CD9+. Only the CD11c+ DC expressed macrophage colony-stimulating factor (M-CSF) receptor and gave rise to CD14+, esterase+, phagocytic macrophages when cultured in M-CSF. These data suggest that these two populations of DC represent distinct lineages of antigen-presenting DC.
authors.affiliation: Antigen Presentation Research Group, Imperial College School of Medicine at St Mary's, Northwick Park Institute for Medical Research, Harrow, GB.
authors.forename: S P
authors.initials: SP
authors.lastname: Robinson
authors.forename: S
authors.initials: S
authors.lastname: Patterson
authors.forename: N
authors.initials: N
authors.lastname: English
authors.forename: D
authors.initials: D
authors.lastname: Davies
authors.forename: S C
authors.initials: SC
authors.lastname: Knight
authors.forename: C D
authors.initials: CD
authors.lastname: Reid
mesh_terms: D000954:Antigens, Surface / Q000097:blood; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor