[Cloning and sequencing of variable region genes of HAb25 McAb against hepatocellular carcinoma].
OBJECTIVE
To clone the variable genes of HAb25 McAb against hepatocellular carcinoma.


METHODS
The HAb25 variable region genes were isolated by RT-PCR technique from the HAb25 hybridoma, and its nucleic acid sequences were analyzed by the sanger's dideoxy-mediated chain-termination method.


RESULTS
The gene of VH was 360 bp long encoding 120 amino acids and the gene of VL was 330 bp long encoding 110 amino acids. Only one open reading frame could be found in each of the variable genes. A comparison of the sequences of VH and VL domatins derived from the HAb25 antibody with those of the published mouse Ig genes in GenBank by computer revealed that the VH gene was homologous to the VH186.2 germline gene family (84.00%) and VL gene was homologous to the MMIgGKAVAG(82.00%).


CONCLUSIONS
Both of the two genes are rearranged variable region genes, which are VHDJH3 for the VH and VK J K4 for the VL.
Hu
C
C
Department of Pathology, Forth Military Medical University, Xi'an.
Liu
Y
Y
Gao
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D005803:Genes, Immunoglobulin; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics*; D008113:Liver Neoplasms / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region