title: [B cell acute lymphocytic leukemia with marked leukocytosis and t(3;15)(q27;q2?2)].
abstract: We report on a 16-year-old boy with B cell acute lymphocytic leukemia presenting marked leukocytosis (388,000/microliter) and resistance to multidrug chemotherapy. Karyotypical analysis revealed a novel t(3;15)(q27;q2?2) chromosomal abnormality. Because 3q27 is known to be a locus of the bcl-6 gene, which is frequently involved in B cell malignancies, molecular biological analyses were performed. Although no rearrangement was detected in 5 genes including bcl-6 on 3q27 and 2 genes on 15q2, reverse transcriptase-polymerase chain reaction procedures detected relatively strong mRNA expression of the bcl-6, smrp, dvl3, and tpml genes. These results indicate that immature leukemic cells with CD10 and CD34 positivity and rearrangement of the T cell receptor beta gene may coexist with relatively mature subpopulations that are positive for CD19 and CD20 surface markers, bcl-6 expression, and rearrangement of the gene for immunoglobulin kappa.
authors.affiliation: Department of Internal Medicine, Kawasaki Medical School.
authors.forename: K
authors.initials: K
authors.lastname: Yata
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: T
authors.initials: T
authors.lastname: Sugihara
authors.forename: O
authors.initials: O
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Otsuki
authors.forename: N
authors.initials: N
authors.lastname: Nakazawa
authors.forename: M
authors.initials: M
authors.lastname: Taniwaki
authors.forename: T
authors.initials: T
authors.lastname: Akasaka
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: Y
authors.initials: Y
authors.lastname: Yawata
mesh_terms: D000293:Adolescent; D002884:Chromosomes, Human, Pair 15; D002893:Chromosomes, Human, Pair 3; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D015448:Leukemia, B-Cell / Q000097:blood* / Q000235:genetics; D007964:Leukocytosis / Q000209:etiology*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors