Effect of complex polyphenols and tannins from red wine (WCPT) on chemically induced oxidative DNA damage in the rat.
BACKGROUND
Flavonoids are polyphenolic antioxidants occurring in vegetables and fruits as well as beverages such as tea and wine which have been thought to influence oxidative damage.


OBJECTIVE
We wanted to verify whether a complex mixture of wine tannins (wine complex polyphenols and tannins, WCPT) prevent chemically-induced oxidative DNA damage in vivo.


METHODS
Oxidative DNA damage was evaluated by measuring the ratio of 8-hydroxy-2'-deoxyguanosine (80HdG)/ 2-deoxyguanosine (2dG) x 10(-6) in hydrolyzed DNA using HPLC coupled with electrochemical and UV detectors.


RESULTS
We treated rats with WCPT (57 mg/kg p.o.) for 14 d, a dose 10-fold higher than what a moderate wine drinker would be exposed to. WCPT administration significantly reduced the ratio of 80HdG/2dG x 10(-6) in liver DNA obtained from rats treated with 2-nitropropane (2NP) relative to controls administered 2NP only (33. 3 +/- 2.5 vs. 44.9 +/- 3.2 x 10(-6) 2dG; micro +/- SE; p<0.05). On the contrary, pretreatment with WCPT for 10 d did not protect the colon mucosa from oxidative DNA damage induced by 1, 2-dimethylhydrazine (DMH). 2NP and DMH are hepatic and colon carcinogens, respectively, capable of inducing oxidative DNA damage.


CONCLUSIONS
WCPT have protective action against some types of chemically-induced oxidative DNA damage in vivo.
Casalini
C
C
Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy. maura@server1.pharm.unifi.it
Lodovici
M
M
Briani
C
C
Paganelli
G
G
Remy
S
S
Cheynier
V
V
Dolara
P
P
D019813:1,2-Dimethylhydrazine / Q000009:adverse effects; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002273:Carcinogens / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003106:Colon / Q000503:physiopathology; D004247:DNA / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects* / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D005419:Flavonoids; D007413:Intestinal Mucosa / Q000503:physiopathology; D008099:Liver / Q000503:physiopathology; D008297:Male; D009595:Nitroparaffins / Q000009:adverse effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000378:metabolism / Q000494:pharmacology*; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology*; D059808:Polyphenols; D011407:Propane / Q000009:adverse effects / Q000031:analogs & derivatives; D051381:Rats; D011916:Rats, Inbred F344; D013634:Tannins / Q000378:metabolism / Q000494:pharmacology*; D014920:Wine
D002273:Carcinogens; D005419:Flavonoids; D009595:Nitroparaffins; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013634:Tannins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; C016617:2-nitropropane; D019813:1,2-Dimethylhydrazine; D011407:Propane