Analysis of angiotension-converting enzyme by capillary electrophoresis.
A method is described for determination of serum angiotension-converting enzyme by capillary electrophoresis (CE) based on incubation of the substrate, a synthetic peptide, with the serum outside the capillary and cleaving hippuric acid and a dipeptide. The reaction is stopped by the addition of acetonitrile, followed by injection of the supernatant on the capillary. The acetonitrile allows injection of a large volume of sample on the capillary. Both the substrate and the reaction product (hippuric acid) can be monitored at the same time. The CE step is rapid and can be performed in about 6 min. The CE method compared well to a kinetic assay method (= 0.98).
Shihabi
Z K
ZK
Pathology Department, Wake Forest University Baptist Medical Center, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D013379:Substrate Specificity
D009842:Oligopeptides; C010980:hippuryl-histidyl-leucine; D007703:Peptidyl-Dipeptidase A