title: Analysis of receptor for Vibrio cholerae El tor hemolysin with a monoclonal antibody that recognizes glycophorin B of human erythrocyte membrane.
abstract: El Tor hemolysin (ETH), a pore-forming toxin secreted by Vibrio cholerae O1 biotype El Tor and most Vibrio cholerae non-O1 isolates, is able to lyse erythrocytes and other mammalian cells. To study the receptor for this toxin or the related molecule(s) on erythrocyte, we first isolated a monoclonal antibody, B1, against human erythrocyte membrane, which not only blocks the binding of ETH to human erythrocyte but also inhibits the hemolytic activity of ETH. Biochemical characterization and immunoblotting revealed that this antibody recognized an epitope on the extracellular domain of glycophorin B, a sialoglycoprotein of erythrocyte membrane. Erythrocytes lacking glycophorin B but not glycophorin A were less sensitive to the toxin than were normal human erythrocytes. These results indicate that glycophorin B is a receptor for ETH or at least an associated molecule of the receptor for ETH on human erythrocytes.
authors.affiliation: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Seno
authors.forename: Y
authors.initials: Y
authors.lastname: Tani
authors.forename: T
authors.initials: T
authors.lastname: Honda
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002772:Cholera Toxin / Q000378:metabolism*; D004910:Erythrocyte Membrane; D005677:G(M1) Ganglioside / Q000032:analysis* / Q000276:immunology; D006021:Glycophorins / Q000032:analysis* / Q000276:immunology; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006021:Glycophorins; D006460:Hemolysin Proteins; D011956:Receptors, Cell Surface; C023552:choleragen receptor; D005677:G(M1) Ganglioside; D002772:Cholera Toxin