[The outlook for an improvement in the agents for detection based on immunofluorescence analysis].
The review deals with prospects for improving the agents for rapid indication of biological agents by a combination of currently available immunochemical and molecular genetic analyses and the latest developments in the design of luminescent tracers, techniques of recording superweak luminous fluxes in the photon count mode and laser spectroscopies. Immunosensor technologies and homogeneous immunoassay are shown to be optimal for the studies to rapidly detect pathogens and toxins. It is most advisable to employ highly sensitive, specific, and efficient methods of molecular hybridization and multicomponent solid-phase immunoassay by recording biospecific binding products in the mode of time luminescence resolution for environmental studies and clinical laboratory diagnoses in resident laboratories. Continuous water and air follow-up monitoring involves the analysis by using immunosorbents, as well as immunosensors and flow cell fluorimetry is noted to become an important independent line in the development of biological indication agents under the increasing man-made influence of the environment.
Zlobin
V N
VN
Osin
N S
NS
Pomelova
V G
VG
D000818:Animals; D003264:Containment of Biohazards; D004784:Environmental Monitoring / Q000379:methods / Q000592:standards; D005455:Fluorescent Antibody Technique / Q000592:standards*; D006801:Humans; D007202:Indicators and Reagents / Q000592:standards; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence; D008967:Molecular Biology / Q000379:methods; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents