title: Cytokine response to BCG infection in alcohol-fed mice.
abstract: Alcoholics have increased susceptibility to infections including tuberculosis. Chronic alcohol treatment impairs host response to bovine mycobacterium infection from BCG. This study assesses the role of four cytokines (TNFalpha, IFNgamma, IL-4, and IL-10) in this impaired response. Twenty male C57BL/6 mice were pair-fed on the Lieber DiCarli control (LCD) or ethanol (LED) diets for 28 days. The LED treated subjects ate ad lib and consumed a mean of 13 g/kg/d of ethanol. After 14 days, based on body weight, subjects were randomly divided into four treatment groups of five each. Ten infected with 2x10(6) colony-forming units (CFU) of BCG by tail-vein. On day 28, the mice were sacrificed. Liver was cultured to determine the mycobacteria CFU/g tissue. Spleens were assayed for the levels of TNFalpha, IFNgamma, IL-4, and IL-10 mRNA relative to mRNA levels for a housekeeping gene using a quantitative reverse transcriptase PCR. Without BCG infection, only the mRNA for IFNgamma was increased by LED treatment, 51% (p = 0.0001). BCG infection significantly increased TNFalpha, IFNgamma, and IL-10 mRNA (p<0.0001). IL-4 mRNA decreased (p = 0.0006). Chronic LED plus BCG infection further increased TNFalpha (p = 0.002) and IFN-gamma (p = 0.04); IL-10 was unchanged, whereas IL-4 was marginally further decreased (p = 0.06). CFU/liver increased with LED (mean +/- SD, 72+/-33x10(5) vs. 39+/-17x10(5); p = 0.004). A significant direct correlation was observed between CFU and TNFalpha, r = 0.70, p = 0.03. In conclusion, BCG infection increases TNFalpha, IFNgamma, & IL-10 and decreases IL-4. CFU numbers correlate with mRNA for TNFalpha, and LED inhibits host containment of BCG infection as measured by liver CFU. This study could not identify cytokine alterations in either Th1- or Th2-type immune responses that might contribute to the impaired host response to the BCG infection.
authors.affiliation: Department of Veteran Affairs and Department of Medicine, University of Cincinnati Medical Center, OH 45220, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Mendenhall
authors.forename: F
authors.initials: F
authors.lastname: Finkelman
authors.forename: R T
authors.initials: RT
authors.lastname: Means
authors.forename: K E
authors.initials: KE
authors.lastname: Sherman
authors.forename: V T
authors.initials: VT
authors.lastname: Nguyen
authors.forename: C E
authors.initials: CE
authors.lastname: Grossman
authors.forename: S C
authors.initials: SC
authors.lastname: Morris
authors.forename: S
authors.initials: S
authors.lastname: Rouster
authors.forename: G A
authors.initials: GA
authors.lastname: Roselle
mesh_terms: D000818:Animals; D002417:Cattle; D002492:Central Nervous System Depressants / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D016207:Cytokines / Q000187:drug effects* / Q000276:immunology; D000431:Ethanol / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism / Q000382:microbiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology*; D012333:RNA, Messenger / Q000187:drug effects / Q000276:immunology; D013154:Spleen / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D002492:Central Nervous System Depressants; D016207:Cytokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D000431:Ethanol; D007371:Interferon-gamma