Effects of antioxidant enzyme overexpression on the invasive phenotype of hamster cheek pouch carcinoma cells.
To examine the role of reactive oxygen species on the invasive phenotype of cancer cells, we overexpressed manganese- and copper-zinc-containing superoxide dismutases (MnSOD, CuZnSOD) and catalase (Cat) in hamster cheek pouch carcinoma (HCPC-1) cells in vitro using adenoviral vector-mediated gene transfer. Hamster cheek pouch carcinoma cells were transduced with these adenoviral vector constructs alone, or in combination, at concentrations [i.e., multiplicity of infectivity (MOI)] of 100 MOI each. The Escherichia coli beta-galactosidase reporter construct was used as a control virus. Protein expression was examined by Western blot analysis and enzymatic activities were measured using spectrophotometry. To observe the effects of transgene overexpression on in vitro tumor cell invasion, we used the membrane invasion culture system, an accurate and reliable method for examining tumor cell invasion, in vitro. This assay measures the ability of tumor cells to invade a basement membrane matrix consisting of type IV collagen, laminin, and gelatin. MnSOD overexpression resulted in a 50% increase in HCPC-1 cell invasiveness (p < .001); co-overexpression of MnSOD with Cat partially inhibited this effect (p < .05). Moreover, co-overexpression of both SODs resulted in a significant increase in invasiveness compared with the parental HCPC-1 cells (p < .05). These changes could not be correlated with the 72 kDa collagenase IV or stromolysin activities using zymography, or the downregulation of the adhesion molecules E-cadherin or the alpha4 subunit of the alpha4beta1 integrin. These results suggest that hydrogen peroxide may play a role in the process of tumor cell invasion, but that the process does not rely on changes in matrix metalloproteinase activity in the cells, or the expression of cell adhesion molecules.
Lam
E W
EW
Department of Radiology, University of Iowa, Iowa City, USA.
Zwacka
R
R
Seftor
E A
EA
Nieva
D R
DR
Davidson
B L
BL
Engelhardt
J F
JF
Hendrix
M J
MJ
Oberley
L W
LW
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002374:Catalase / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D006224:Cricetinae; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010641:Phenotype; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D015815:Cell Adhesion Molecules; D002374:Catalase; D013482:Superoxide Dismutase; D008666:Metalloendopeptidases