Peroxisome proliferator-activated receptor gamma1 expression in porcine white blood cells: dynamic regulation with acute endotoxemia.
Peroxisome proliferator-activated receptor gamma (PPARgamma), a primary regulator of adipocyte differentiation, has been implicated in the regulation of monocyte and macrophage function in vitro. We report that PPARgamma protein is expressed in porcine peripheral white blood cells (WBC), and that PPARgamma1 but not gamma2 mRNA predominates. Additionally, we provide the first evidence that in vivo lipopolysaccharide challenge (LPS, 25 microg/kg BW) causes a dynamic increase in PPARgamma protein expression in peripheral WBC (P < 0.05). PPARgamma expression was increased 2-fold over basal (1 hr post-LPS), was maximal by 4 hr (3-fold), and was normalized to control by 8 hr post-LPS. Changes in PPARgamma expression coincided with or closely followed LPS-induced changes in plasma cortisol, TNF-alpha, insulin, IGF-1, glucose, and free fatty acids. These data suggest that induction of PPARgamma expression in WBC may play a role in host response to acute inflammatory challenge and may prove to be an important target of anti-inflammatory therapies.
Leininger
M T
MT
Department of Animal Sciences, Purdue University, West Lafayette, Indiana, USA.
Portocarrero
C P
CP
Houseknecht
K L
KL
D000818:Animals; D004268:DNA-Binding Proteins / Q000097:blood / Q000235:genetics; D019446:Endotoxemia / Q000097:blood*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D009919:Orchiectomy; D020033:Protein Isoforms / Q000097:blood / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000097:blood* / Q000235:genetics*; D012016:Reference Values; D013552:Swine; D013997:Time Factors; D014157:Transcription Factors / Q000097:blood* / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D004268:DNA-Binding Proteins; D008070:Lipopolysaccharides; D020033:Protein Isoforms; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors