Y receptor-mediated induction of CD63 transcripts, a tetraspanin determined to be necessary for differentiation of the intestinal epithelial cell line, hBRIE 380i cells.
Peptide YY (PYY) and neuropeptide Y (NPY) are peptides that coordinate intestinal activities in response to luminal and neuronal signals. In this study, using the rat hybrid small intestinal epithelial cell line, hBRIE 380i cells, we demonstrated that PYY- and NPY-induced rearrangement of actin filaments may be in part through a Y1alpha and/or a nonneuronal Y2 receptor, which were cloned from both the intestinal mucosa and the hBRIE 380i cells. A number of PYY/NPY-responsive genes were also identified by subtractive hybridization of the hBRIE 380i cells in the presence or absence of a 6-h treatment with PYY. Several of these genes coded for proteins associated with the cell cytoskeleton or extracellular matrix. One of these proteins was the transmembrane-4 superfamily protein CD63, previously shown to associate with beta(1)-integrin and implicated in cell adhesion. CD63 immunoreactivity, using antibody to the extracellular domain, was highest in the differentiated cell clusters of the hBRIE 380i cells. The hBRIE 380i cells transfected with antisense CD63 cDNA lost these differentiated clusters. These studies suggest a new role for NPY and PYY in modulating differentiation through cytoskeletal associated proteins.
Halldén
G
G
Department of Nutritional Sciences, University of California, Berkeley, California 94720-3104, USA.
Hadi
M
M
Hong
H T
HT
Aponte
G W
GW
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000235:genetics; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006822:Hybrid Cells; D019012:Integrin beta1 / Q000235:genetics / Q000502:physiology; D007413:Intestinal Mucosa; D007421:Intestine, Small; D007700:Kinetics; D009478:Neuropeptide Y / Q000494:pharmacology*; D019894:Peptide YY / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011964:Receptors, Gastrointestinal Hormone / Q000502:physiology*; D017476:Receptors, Neuropeptide Y / Q000502:physiology*; D060149:Tetraspanin 30; D014158:Transcription, Genetic / Q000187:drug effects
D015703:Antigens, CD; C554588:Cd63 protein, rat; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D019012:Integrin beta1; D009478:Neuropeptide Y; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011964:Receptors, Gastrointestinal Hormone; D017476:Receptors, Neuropeptide Y; D060149:Tetraspanin 30; C082172:peptide YY receptor; D019894:Peptide YY