[Relationship between oxidoreductase activity and cytochrome b5 and P-450 levels in Pseudomonas bacteria depending upon the structure of C6-substrate].
The influence of the structure of C6 growth substrate on the activity of oxidoreductases and content of cytochromes b5 and P-450 in cells of Pseudomonas aeruginosa and P. fluorescens was studied. Among the hydrocarbons tested (hexane, 1-hexene, cyclohexene), 1-hexene was the most efficient inducer of the synthesis of cytochromes b5 and P-450 in log-phase cells of the pseudomonads. Substitution of the hydrocarbon substrate for a carbohydrate one (glucose) also resulted in a considerable increase in the activity of enzymes of the NADH-dependent electron-transport chain.
Sokol'chik
T I
TI
Belarussian State Technological University, Minsk, Belarus.
Leont'ev
V N
VN
Grits
N V
NV
D000475:Alkenes / Q000737:chemistry; D003510:Cyclohexanes / Q000737:chemistry; D053138:Cyclohexenes; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D015786:Cytochromes b5 / Q000378:metabolism*; D005947:Glucose / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D010088:Oxidoreductases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000254:growth & development*; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000254:growth & development*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D000475:Alkenes; D003510:Cyclohexanes; D053138:Cyclohexenes; D006586:Hexanes; C052568:cyclohexene; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; C117224:1-hexene; D010088:Oxidoreductases; D005947:Glucose