ACE, PAI-1, decorin and Werner helicase genes are not associated with the development of renal disease in European patients with type 1 diabetes.
BACKGROUND
Genetic factors are involved in the development of diabetic nephropathy in Type 1 diabetes. We have examined the association of four candidate genes, angiotensin converting enzyme (ACE): insertion/deletion (I/D) polymorphism, plasminogen activator inhibitor-1 (PAI-1): 4G/5G polymorphism, decorin: 179/183/185 polymorphism and Werner syndrome helicase: C/R polymorphism, with the presence of diabetic nephropathy in Type 1 diabetic patients.


METHODS
175 Type 1 diabetic patients with albuminuria (59 with microalbuminuria and 116 with macroalbuminuria) were compared with 136 Type 1 diabetic patients with normoalbuminuria and duration of disease longer than 15 years (mean+/-SD: 25+/-8 years). 200 non-diabetic subjects were also studied as background population.


RESULTS
We found no association in the polymorphism of the four genes examined between patients with and without diabetic nephropathy and the control subjects.


CONCLUSIONS
The genes studied are unlikely to be involved in the susceptibility to nephropathy in Type 1 diabetic patients.
De Cosmo
S
S
Division and Research Unit of Endocrinology, Scientific Institute 'Casa Sollievo della Sofferenza', San Giovanni Rotondo (FG), Italy. sdecosm@tin.it
Margaglione
M
M
Tassi
V
V
Garrubba
M
M
Thomas
S
S
Olivetti
C
C
Piras
G P
GP
Trevisan
R
R
Vedovato
M
M
Cavallo Perin
P
P
Bacci
S
S
Colaizzo
D
D
Cisternino
C
C
Zucaro
L
L
Di Minno
G
G
Trischitta
V
V
Viberti
G C
GC
D000328:Adult; D000483:Alleles; D004265:DNA Helicases / Q000235:genetics*; D058575:Decorin; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000235:genetics*; D005090:Exodeoxyribonucleases; D016326:Extracellular Matrix Proteins; D005260:Female; D005838:Genotype; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011509:Proteoglycans / Q000235:genetics*; D053484:RecQ Helicases; D006113:United Kingdom; D014898:Werner Syndrome / Q000201:enzymology; D000071657:Werner Syndrome Helicase
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D017395:Plasminogen Activator Inhibitor 1; D011509:Proteoglycans; D005090:Exodeoxyribonucleases; D007703:Peptidyl-Dipeptidase A; D004265:DNA Helicases; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase