title: Effect of isolation techniques, in vitro culture and IFNgamma treatment on the constitutive expression of MHC Class I and Class II molecules on goat neutrophils.
abstract: Previous studies on the ability of human neutrophils to synthesize cytokines and express MHC Class I and inducible Class II molecules have suggested a possible role of these cells as accessory or antigen presenting cells (APC). There is no information available to date concerning this aspect in ruminants. Therefore, as the first step of these investigations, we have studied the cell surface expression of MHC molecules on goat neutrophils. We show that goat neutrophils can be distinguished from eosinophils with monoclonal antibody (MoAb) ILA-24 which recognizes cattle monocytes and neutrophils. Goat neutrophils constitutively express MHC Class II molecules. However, cell surface expression of MHC Class I and Class II molecules is dramatically reduced on neutrophils purified by density gradient centrifugation in comparison to neutrophils obtained from whole blood after lysis of erythrocytes. Also, the level of expression of MHC Class I antigens is seasonal and donor-dependent and rapidly decreases after in vitro culture despite negligible necrosis and apoptosis of neutrophils. Although treatment with IFNgamma partially prevents the loss of MHC Class I molecules on neutrophils, it fails to induce MHC Class II antigens. Implications of these results for further studies on the potential role of neutrophils as APC are discussed.
authors.affiliation: CIRAD-EMVT, Domaine Duclos, Petit Bourg, Guadeloupe.
authors.forename: N
authors.initials: N
authors.lastname: Vachiéry
authors.forename: P
authors.initials: P
authors.lastname: Totté
authors.forename: V
authors.initials: V
authors.lastname: Balcer
authors.forename: D
authors.initials: D
authors.lastname: Martinez
authors.forename: A
authors.initials: A
authors.lastname: Bensaid
mesh_terms: D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000662:veterinary; D002478:Cells, Cultured; D004804:Eosinophils / Q000737:chemistry* / Q000187:drug effects; D005434:Flow Cytometry / Q000662:veterinary; D006041:Goats / Q000097:blood*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000097:blood; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects; D009504:Neutrophils / Q000737:chemistry* / Q000187:drug effects
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma