Differential expression of protein kinase C isoform transcripts in human hematopoietic progenitors undergoing differentiation.
Protein kinase C (PKC), a key component of the signaling pathways leading to proliferation and differentiation, consists of a family closely related serine/threonine protein kinases. The mRNA expression of these PKC isoforms has been characterized during hematopoietic differentiation. Using the reverse-transcriptase polymerase chain reaction technique, we have analyzed the levels of isoform transcripts in bone marrow CD34(+) hematopoietic progenitors and their progeny differentiated along erythroid, megakaryocyte, or granulocyte/monocyte lineages, upon exposure to growth factors. In contrast with isoforms alpha, beta(I), beta(II), delta, and epsilon, ubiquitously expressed, isoforms theta, eta/L, zeta, and iota/lambda exhibited a lineage-restricted expression. These qualitative changes, which allow to distinguish the erythroid and megakaryocyte phenotypes from the granulocyte/monocyte phenotype, include zeta exclusively upregulated in granulocytes/monocytes and theta, eta/L, and iota/lambda exclusively expressed in megakaryocytes and erythroblasts. In contrast, erythroblasts and megakaryocytes, which supposedly share a common bipotential progenitor, displayed only quantitative changes. These results evidence the selective expression of PKC isoforms at transcriptional and/or posttranscriptional levels in hematopoietic progenitors induced to differentiate, which may suggest a differential contribution of individual isoforms to cellular signaling.
Oshevski
S
S
Hôpital Paul Brousse, INSERM U 268, Villejuif cedex, 94807, France.
Le Bousse-Kerdilès
M C
MC
Clay
D
D
Levashova
Z
Z
Debili
N
N
Vitral
N
N
Jasmin
C
C
Castagna
M
M
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D001792:Blood Platelets / Q000201:enzymology; D001854:Bone Marrow Cells / Q000166:cytology / Q000201:enzymology*; D001921:Brain / Q000201:enzymology; D002454:Cell Differentiation; D017931:DNA Primers; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D006098:Granulocytes / Q000166:cytology / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008533:Megakaryocytes / Q000166:cytology / Q000201:enzymology; D009000:Monocytes / Q000166:cytology / Q000201:enzymology; D011493:Protein Kinase C / Q000235:genetics*; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D015703:Antigens, CD; D018952:Antigens, CD34; D017931:DNA Primers; D007527:Isoenzymes; C496628:PRKCA protein, human; C497258:PRKCD protein, human; C497255:PRKCE protein, human; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon