Control of photoinduced electron transfer from zinc-porphyrin to methyl viologen by supramolecular formation between monoclonal antibody and zinc-porphyrin.
Photoinduced electron transfer from tetrakis(4-carboxy-phenyl porphyrin)-zinc complex (Zn-TCPP) to an acceptor molecule (methyl viologen; MV2+) has been found to be controlled by the complex formation of monoclonal antibody 03-1 for the porphyrin (TCPP) and Zn-TCPP. Although there are no ground-state interactions between Zn-TCPP and MV2+ for a 2:1 complex of antibody 03-1 and Zn-TCPP, the fluorescence of Zn-TCPP is quenched by the addition of MV2+. The Stern-Volmer plots and emission lifetime studies show that there is a long-range electron transfer through the antibody 03-1.
Harada
A
A
Department of Macromolecular Science, Graduate School of Science, Osaka University, Japan. harada@chem.sci.osaka-u.ac.jp
Yamaguchi
H
H
Okamoto
K
K
Fukushima
H
H
Shiotsuki
K
K
Kamachi
M
M
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001665:Binding Sites; D015374:Biosensing Techniques; D004583:Electrons; D006540:Herbicides / Q000737:chemistry*; D046911:Macromolecular Substances; D008665:Metalloporphyrins / Q000737:chemistry*; D010269:Paraquat / Q000737:chemistry*; D010777:Photochemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D006540:Herbicides; D046911:Macromolecular Substances; D008665:Metalloporphyrins; C067542:zinc(II) tetrakis(4-carboxyphenyl)porphine; D010269:Paraquat