The time-dependent difference of GAP-43 expression between sensory neurons and motoneurons after peripheral nerve transection.
The L5 dorsal root ganglion (DRG) cells and L5 anterior horn (AH) cells of rats were studied and examined immunocytochemically after transection of the sciatic nerve to find out whether there would be time-dependent differences in the increase of growth-associated protein (GAP-43) expression between sensory neurons and motoneurons. On the seventh day after transection at mid-thigh level, the levels of GAP-43 in the DRG cells significantly increased, while those in the AH cells began to increase gradually from the 14th day onward. Transection at the piriform muscle level induced a significant increase in immunoreactivity of GAP-43 on the third day in the DRG cells, and on the seventh day in the AH cells. These results showed that sensory neurons expressed GAP-43 immunoreactivity earlier than motoneurons after peripheral nerve transection regardless of the site, suggesting that sensory neurons might start to produce cytoskeletons for axonal elongation earlier than motoneurons after nerve transection.
Matsuura
Y
Y
Department of Orthopaedics, Shimane Medical University, Shimane-ken, Japan.
Ochi
M
M
Uchio
Y
Y
Suzuki
G
G
Iwata
A
A
D000704:Analysis of Variance; D000818:Animals; D019922:GAP-43 Protein / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000378:metabolism* / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D009475:Neurons, Afferent / Q000378:metabolism* / Q000502:physiology; D010525:Peripheral Nerves / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000502:physiology; D013997:Time Factors
D019922:GAP-43 Protein