Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).
A widely applicable, positive cDNA selection method was developed to identify RNAs synthesized by Mycobacterium tuberculosis in response to phagocytosis by cultured human primary macrophages. cDNAs for sigE and sigH (alternative sigma factors), aceA (isocitrate lyase), ponA (class I penicillin-binding protein), pks2 (polyketide synthase), uvrA (UvrABC endonuclease), and ctpV (putative cation transporter) were obtained from macrophage-grown bacteria. cDNAs for ORFs Rv3070, Rv3483c, Rv0903c (encoding a putative bacterial two-component transcriptional activator), and Rv0170 of the mce1 virulence operon also were obtained from phagocytized bacilli. cDNAs for these genomic regions were not obtained from approximately 1, 000-fold more bacteria grown in laboratory broth. Methods described here, which have identified M. tuberculosis genes expressed in response to host interaction, will allow the study of gene expression in a variety of microorganisms, including expression resulting from interaction with human tissues in natural disease states.
Graham
J E
JE
Department of Biology, Washington University, St. Louis, MO 63130, USA.
Clark-Curtiss
J E
JE
D001426:Bacterial Proteins / Q000235:genetics; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000032:analysis; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology; D010587:Phagocytosis; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012808:Sigma Factor / Q000235:genetics; D015534:Trans-Activators
D001426:Bacterial Proteins; D018076:DNA, Complementary; C085225:REGA protein, Rhodobacter sphaeroides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012808:Sigma Factor; D015534:Trans-Activators; C111259:sigE protein, Bacteria