Effect of a dietary nonionic surfactant on small intestinal nutrient transport.
Nonionic surfactants are commonly present in many prepared foods and drug formulations as stabilizing agents. The aim of the current study was to examine the effect of the common nonionic surfactant Tween-80 on jejunal glucose transport. New Zealand White rabbits (800-1200 g) were fasted for 24 hr. Jejunal tissue was stripped and mounted in short-circuited Ussing chambers. Unidirectional 3-O-methyl glucose fluxes were determined during a 15-min basal transport period and a subsequent 15-min experimental period after the mucosal addition of Tween-80 at final concentrations of 0.001%, 0.01%, 0.05%, and 0.1%. Tween-80 at final concentrations of 0.1% and 0.05% significantly increased net 3-O-methyl glucose transport over basal levels. The increase in Jnet was due to a significant increase in the absorptive Jm-s flux. Tween-80 at 0.01% and 0.001% did not significantly alter net glucose transport although Jm-s was significantly increased in the presence of 0.01% Tween-80. Tween-80, in concentrations commonly found in prepared foods, enhances the intestinal absorption of glucose.
Donegan
L
L
Gastrointestinal Research Group, University of Calgary, Alberta, Canada.
Hudye
S A
SA
Olson
M E
ME
Hardin
J A
JA
Gall
D G
DG
D019325:3-O-Methylglucose / Q000378:metabolism; D000818:Animals; D005950:Glucose Solution, Hypertonic / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects*; D008871:Microvilli / Q000187:drug effects; D011136:Polysorbates / Q000494:pharmacology*; D011817:Rabbits; D013501:Surface-Active Agents / Q000494:pharmacology*
D005950:Glucose Solution, Hypertonic; D011136:Polysorbates; D013501:Surface-Active Agents; D019325:3-O-Methylglucose