Additive effect of Sp1 and Sp3 in regulation of the ED-L1E promoter of the EBV LMP 1 gene in human epithelial cells.
The ED-L1E promoter of the LMP 1 gene is a GC box-containing promoter. To test if Sp1/Sp3 are important for modulating ED-L1E promoter activity through the GC box, site-specific mutation and deletion constructs carrying a reporter gene were transfected into NPCTW076 and C33A cells. Results showed that deletion or mutation of the GC box abolished ED-L1E activity. Association of Sp1/Sp3 with the GC box was confirmed by electrophoretic mobility shift and supershift assays using Sp1- and Sp3-specific antibodies. Transfection of Sp1- and Sp3-expressing vectors into NPCTW076 and Sp-deficient Drosophila SL2 cells activated ED-L1E in a dose-dependent fashion and showed an additive effect. Data suggest that both factors may function as transcriptional activators and regulate the ED-L1E promoter in human epithelial cells.
Tsai
C N
CN
Department of Microbiology and Immunology, Chang-Gung University School of Medicine, Kwei-shan, Taoyuan, Taiwan, Republic of China.
Lee
C M
CM
Chien
C K
CK
Kuo
S C
SC
Chang
Y S
YS
D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D004847:Epithelial Cells / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006566:Herpesviridae Infections / Q000235:genetics* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000235:genetics; D051705:Sp3 Transcription Factor; D014157:Transcription Factors / Q000235:genetics; D014412:Tumor Virus Infections / Q000235:genetics* / Q000821:virology; D014763:Viral Matrix Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; C497121:SP3 protein, human; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D014763:Viral Matrix Proteins; D051705:Sp3 Transcription Factor