Engineering parvovirus-like particles for the induction of B-cell, CD4(+) and CTL responses.
An antigen delivery system based on hybrid recombinant parvovirus-like particles (VLPs) formed by the self-assembly of the capsid VP2 protein of porcine (PPV) or canine parvovirus (CPV) expressed in insect cells with the baculovirus system has been developed. PPV:VLPs containing a CD8(+) epitope from the LCMV nucleoprotein evoked a potent CTL response and were able to protect mice against a lethal infection with the virus. Also, PPV:VLPs containing the C3:T epitope from poliovirus elicited a CD4(+)3 log(10) units) against poliovirus. The possibility of combining different types of epitopes in different positions of a single particle to stimulate different branches of the immune system paves the way to the production of more potent vaccines in a simple and cheap way.
Rueda
P
P
Immunologia y Genetica Apl. S.A. (INGENASA), Hnos, García Noblejas 41, 4 degrees. 28037, Madrid, Spain.
Martínez-Torrecuadrada
J L
JL
Sarraseca
J
J
Sedlik
C
C
del Barrio
M
M
Hurtado
A
A
Leclerc
C
C
Casal
J I
JI
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004285:Dogs; D000939:Epitopes; D018655:Lymphocyte Count; D051379:Mice; D017992:Parvovirus; D017993:Parvovirus, Canine; D015202:Protein Engineering; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
D000939:Epitopes; D014764:Viral Proteins