Phosphatidic acid is important to the translocation of Rab3A from the cytosol to phospholipid membranes.
RAB3A, a Ras-related GTP-binding protein, is found in a rat neuronal cytosol in the form of complex with RabGDI or located on the synaptic vesicles and it cycles between the cytosol and the membranes. However, the regulatory mechanism of the translocation of Rab3A has not been clearly understood. To understand the mechanism of the translocation of Rab3A from the cytosol to the membranes, we examined which membranenous phospholipid is required for the translocation of Rab3A. Phosphatidic acid (PA) was found to be required for the translocation of Rab3A to the membranes and GTPgammaS stimulated the translocation of Rab3A.
Jung
Y J
YJ
Department of Biochemistry, College of Medicine, Hallym University, Chun-Chon, Kang-Won Do, South Korea.
Lee
T H
TH
Lee
J Y
JY
Kim
J H
JH
Park
J B
JB
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D003600:Cytosol / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D010712:Phosphatidic Acids / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D020826:rab3A GTP-Binding Protein / Q000378:metabolism*
D010712:Phosphatidic Acids; D010743:Phospholipids; D011994:Recombinant Proteins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020826:rab3A GTP-Binding Protein