Morphine reduces pulmonary inflammation in response to influenza infection.
The present study shows that morphine reduces the pulmonary inflammatory response to intranasal influenza virus infection in rats. Rats were infected with rat-adapted influenza virus (RAIV), which is a unique infectious agent because normal rats develop an acute pulmonary inflammatory response to RAIV and rapidly clear the virus within a few days with no mortality. Male Lewis rats were implanted with 75 mg morphine pellets or placebo pellets 72 hours prior to intranasal RAIV infection. Rats were euthanized at 2, 24, 48, 72, and 96 hours after infection. Assessment of inflammation included accumulation of inflammatory cells in the lungs, lung weight, and protein and LDH content of bronchial alveolar lavage fluid (BALF). Placebo-treated rats showed a marked inflammatory response to RAIV infection, and morphine-treated rats mounted less vigorous inflammatory responses to the infection. Taken together, these data suggest that morphine treatment impairs the inflammatory response to RAIV infection in the lungs, which is consistent with prior work demonstrating that morphine is a potent anti-inflammatory agent in other areas of the body.
Coussons-Read
M E
ME
Department of Psychology, University of Colorado at Denver 80217, USA. mcousson@carbon.cudenver.edu
Daniels
M
M
Gilmour
M I
MI
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D009980:Influenza A virus; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009976:Orthomyxoviridae Infections / Q000188:drug therapy*; D011024:Pneumonia, Viral / Q000188:drug therapy*; D011506:Proteins / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew
D000893:Anti-Inflammatory Agents; D011506:Proteins; D009020:Morphine; D007770:L-Lactate Dehydrogenase