Comparison of potential pathogenic traits of staphylococci that may contribute to corneal ulceration and inflammation.
Staphylococcus epidermidis and Staphylococcus aureus are two of the commonest bacteria isolated from corneal ulcers. The aim of the current investigation was to determine the frequency of potentially pathogenic traits in the two staphylococcal species. Strains of both species, some isolated from eyes during active corneal inflammation, were screened for their ability to degrade a variety of proteins and hyaluronic acid and the production of cytotoxins. S. aureus produced more tissue-destructive enzymes than the S. epidermidis. S. aureus strains more commonly synthesized the cytotoxin, alpha-toxin. The production of elastase was more common among S. aureus strains isolated from ulcerative events. The production of alpha-toxin was inversely correlated with isolation from keratitis. This study has demonstrated that S. aureus is better equipped with a range of potentially damaging enzymes and toxins than S. epidermidis and that S. aureus elastase may be involved in the production of corneal ulcers.
Wu
P Z
PZ
Cooperative Research Centre for Eye Research and Technology, School of Optometry, University of New South Wales, Sydney, Australia. p.wu@cclru.randwick.unsw.edu.au
Zhu
H
H
Thakur
A
A
Willcox
M D
MD
D001427:Bacterial Toxins / Q000096:biosynthesis; D003320:Corneal Ulcer / Q000382:microbiology*; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D007634:Keratitis / Q000382:microbiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D013210:Staphylococcus / Q000472:pathogenicity*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D001427:Bacterial Toxins; D010738:Type C Phospholipases; D010450:Endopeptidases; D010196:Pancreatic Elastase