title: Involvement of adenosine cyclic-3',5'-monophosphate in the signal transduction pathway of mandibular organ-inhibiting hormone of the edible crab, Cancer pagurus.
abstract: The juvenoid, methyl farnesoate (MF), is synthesized in the mandibular organs (MOs) of crustaceans, under the control of mandibular organ-inhibiting hormone (MO-IH). Using an in vitro assay to measure synthesis of MF by MOs, the effect of a variety of agents that affect signal transduction pathways was investigated. Of the compounds tested, only agents which affect cAMP (forskolin and 8-bromoadenosine cyclic-3',5'-monophosphate) levels were found to mimic the inhibitory action of MO-IH on MF synthesis. To further support these findings, the effect of MO-IH-1 on production of cAMP was investigated. The results demonstrated that MO-IH stimulated a dose-dependent increase in cAMP levels. Furthermore, a maximal 2-fold increase in cAMP was detected after a 5-min exposure of MO membranes to 100 nM MO-IH-1, falling to basal levels thereafter. The results presented strongly support a role for cAMP in the signal transduction mechanism of MO-IH that leads to inhibition of MF synthesis in MOs.
authors.affiliation: School of Biological Sciences, University of Liverpool, UK. geoffw@liv.ac.uk
authors.forename: G
authors.initials: G
authors.lastname: Wainwright
authors.forename: S G
authors.initials: SG
authors.lastname: Webster
authors.forename: H H
authors.initials: HH
authors.lastname: Rees
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D005260:Female; D066298:In Vitro Techniques; D008334:Mandible / Q000378:metabolism; D009479:Neuropeptides / Q000187:drug effects* / Q000378:metabolism* / Q000494:pharmacology; D015290:Second Messenger Systems; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D005231:Fatty Acids, Unsaturated; D009479:Neuropeptides; C100331:mandibular organ-inhibiting hormone 1; C032429:methyl farnesoate; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP