Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso.
African animal trypanosomoses constitute the most important vector-borne cattle diseases in sub-Saharan Africa. Generally it is considered that there is a great lack of accurate tools for the diagnosis of the disease. During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina Faso, 1036 cattle were examined for trypanosomes using microscopy. The PCR was applied on a subset of 260 buffy-coat samples using primers specific for Trypanosoma congolense savannah and riverine-forest groups, T. vivax, and T. brucei. Parasitological examination and the molecular technique were compared, showing a better efficiency of the latter. In the near future, the PCR is likely to become an efficient tool to estimate the prevalence of African trypanosomoses in affected areas.
Solano
P
P
CIRAD-EMVT campus de Baillarguet, Montpellier, France. solano@mpl.ird.fr
Michel
J F
JF
Lefrançois
T
T
de La Rocque
S
S
Sidibé
I
I
Zoungrana
A
A
Cuisance
D
D
D000818:Animals; D002050:Burkina Faso / Q000453:epidemiology; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D006400:Hematocrit / Q000662:veterinary; D008853:Microscopy / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D014345:Trypanosoma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000302:isolation & purification; D014348:Trypanosoma congolense / Q000235:genetics / Q000302:isolation & purification; D016689:Trypanosoma vivax / Q000235:genetics / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis* / Q000453:epidemiology
D017931:DNA Primers; D016054:DNA, Protozoan