title: Expression of urease does not affect the ability of Bordetella bronchiseptica to colonise and persist in the murine respiratory tract.
abstract: To investigate the role played by urease during the Bordetella bronchiseptica infection process, the ability to colonise and persist in the mouse respiratory tract of a urease-negative B. bronchiseptica BB7865 and a BB7865 derivative constitutively expressing urease was compared with that of the wild-type strain. The results obtained showed that neither constitutive expression nor abolishment of urease activity had any significant effect on the course of B. bronchiseptica infection. Therefore, under our experimental conditions, urease is not essential for B. bronchiseptica to colonise and persist within the murine host.
authors.affiliation: Department of Biological Sciences, University of Wollongong, NSW, Australia.
authors.forename: D J
authors.initials: DJ
authors.lastname: McMillan
authors.forename: E
authors.initials: E
authors.lastname: Medina
authors.forename: C A
authors.initials: CA
authors.lastname: Guzmán
authors.forename: M J
authors.initials: MJ
authors.lastname: Walker
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001885:Bordetella Infections / Q000382:microbiology*; D016950:Bordetella bronchiseptica / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D015169:Colony Count, Microbial; D005260:Female; D008168:Lung / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012141:Respiratory Tract Infections / Q000382:microbiology*; D013997:Time Factors; D014510:Urease / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D014510:Urease