Genetically engineered brain drug delivery vectors: cloning, expression and in vivo application of an anti-transferrin receptor single chain antibody-streptavidin fusion gene and protein.
A single chain Fv antibody-streptavidin fusion protein was expressed and purified from bacterial inclusion bodies following cloning of the genes encoding the variable region of the heavy chain and light chain of the murine OX26 monoclonal antibody to the rat transferrin receptor. The latter undergoes receptor mediated transcytosis through the brain capillary endothelial wall in vivo, which makes up the blood-brain barrier (BBB); therefore, the OX26 monoclonal antibody and its single chain Fv analog may act as brain drug delivery vectors in vivo. Attachment of biotinylated drugs to the antibody vector is facilitated by production of the streptavidin fusion protein. The bi-functionality of the OX26 single chain Fv antibody-streptavidin fusion protein was retained, as the product both bound biotin and the rat transferrin receptor in vitro and in vivo, based on pharmacokinetic and brain uptake analyses in anesthetized rats. The attachment of biotin-polyethyleneglycol-fluorescein to the OX26 single chain Fv antibody-streptavidin fusion protein resulted in illumination of isolated rat brain capillaries in confocal fluorescent microscopy. In conclusion, these studies demonstrate that genetically engineered single chain Fv antibody-streptavidin fusion proteins may be used for non-invasive neurotherapeutic delivery to the brain using endogenous BBB transport systems such as the transferrin receptor.
Li
J Y
JY
Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90095, USA.
Sugimura
K
K
Boado
R J
RJ
Lee
H J
HJ
Zhang
C
C
Duebel
S
S
Pardridge
W M
WM
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D001483:Base Sequence; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004337:Drug Carriers; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011990:Receptors, Transferrin / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000493:pharmacokinetics; D019809:Streptavidin / Q000235:genetics*
D000906:Antibodies; D004274:DNA, Recombinant; D004337:Drug Carriers; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins; D019809:Streptavidin