title: The influence of exogenous steroid hormones on steroid receptors, uterine histological structure and the bacterial flora of the normal bitch.
abstract: Oestrogen (ER) and progesterone (PR) receptors have been shown to vary in both concentration and distribution during the oestrous cycle of the bitch, influenced by the normal changes in endogenous reproductive hormones. The influence of exogenous steroid hormones on steroid receptors and the histological structure of the uterus was studied in two groups of parous Beagle bitches. Group A (n = 6) were treated with progesterone (P4) in oil i.m. (3 mg/kg) in late metoestrus on the day that peripheral plasma P4 concentrations were first identified as <10 ng/ml, and subsequently once weekly on three other occasions. Group B (n = 6) were treated with a single i.m. injection of MPA (50 mg, 4.2-5.6 mg/kg) following the same protocol. Full-thickness uterine wall biopsies were obtained from the mid part of one horn 2-7 days after the last (fourth) injection of P4 or MPA. During the subsequent oestrus, when peripheral plasma P4 concentrations were between 8 and 10 ng/ml, each bitch in both groups (n = 12) received a single injection of oestradiol benzoate (ODB) in oil i.m. (7.5 mg, 0.63-0.84 mg/kg). All bitches had an ovariohysterectomy 7 days later. Full-thickness uterine wall samples were obtained from the mid part of the intact horn and other parts of the uterus. Swabs were taken from the uterine lumen for bacteriological examination; all were sterile. Tissue samples were sectioned and examined for evidence of lesions, and stained for ER and PR receptors using an immunocytochemical method. The immunoreactivity was scored semiquantitatively, incorporating both the intensity and distribution of specific staining of the receptors using a simplified histoscore (H-score). At the time of ovariohysterectomy, fluid had accumulated in the isolated section of the uterine horn distal to the point of biopsy; the volume was greater in the MPA-treated bitches. There was also evidence in some sections of histological changes in the endometrium. Variations in the expression of both ER and PR were seen between bitches, which may have been due to some not being in mid-metoestrus at the time of treatment. In general, ER scores were low after P4 and MPA treatment, but following ODB there was a significant (P<0.05) increase in ER expression in all parts of the endometrium. PR scores were zero in the glandular epithelium of all 12 bitches after P4, MPA and ODB treatment, whereas in the other parts of the endometrium they were generally moderate to high. Following treatment with ODB, PR generally increased in the three regions of the endometrium where PR were present. The study shows that ER and PR distribution and expression in the endometrium of bitches can be modified by P4, MPA and ODB, with evidence of individual variation.
authors.affiliation: Department of Farm Animal and Equine Medicine and Surgery, Royal Veterinary College (University of London), Hatfield, Herts, UK.
authors.forename: G K
authors.initials: GK
authors.lastname: Dhaliwal
authors.forename: G C
authors.initials: GC
authors.lastname: England
authors.forename: D E
authors.initials: DE
authors.lastname: Noakes
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D004285:Dogs / Q000033:anatomy & histology* / Q000382:microbiology / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology; D005260:Female; D007044:Hysterectomy / Q000662:veterinary; D007150:Immunohistochemistry; D008525:Medroxyprogesterone / Q000494:pharmacology; D011374:Progesterone / Q000097:blood / Q000494:pharmacology* / Q000502:physiology; D011863:Radioimmunoassay / Q000662:veterinary; D011897:Random Allocation; D011960:Receptors, Estrogen / Q000032:analysis / Q000502:physiology*; D011980:Receptors, Progesterone / Q000032:analysis / Q000502:physiology*; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol; D008525:Medroxyprogesterone