Determination of amoxicillin in plasma samples by capillary electrophoresis.
We have developed a capillary zone electrophoresis (CZE) method for determining amoxicillin in animal plasma samples. Sample clean-up involved solid-phase extraction onto Sep-Pak C18 cartridges followed by elution with water-methanol (85:15). This paper describes two different techniques to increase the sensitivity of the CZE method: field-amplified sample injection (FASI) and electrokinetic injection. We have enhanced the detection limit to 280 micrograms l-1 by the FASI technique.
Hernández
M
M
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Borrull
F
F
Calull
M
M
D000658:Amoxicillin / Q000097:blood*; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000658:Amoxicillin