title: [Effect of electrolyte composition of various solutions on potential-sensitive ion channels, formed by syringomycin E in lipid bilayers].
abstract: Using the planar lipid bilayer technique, the dependence of properties of ion channels formed by syringomycin E on bath ion composition (electrolyte concentration and pH) and on the applied potential was studied. Time courses of the membrane current in field reversal experiments with 1M NaCl, pH 6 in the bathing solution were similar to the time courses observed with a bathing solution of 0.1 M NaCl, pH 2: there was no increase (decrease) of the membrane current at positive (negative) values of the transmembrane potential. However, kinetic curves were different at 0.1 M. NaCl, pH 6 and pH 9. At pH 6 there was an abrupt increase (decrease) of the membrane current over the time at the positive (negative) values of the applied potential. At pH 9 there was a reversed current response. The results indicate that charged groups are likely to be responsible for opening or closing the channel facing the water phase. Based on these data, a model is proposed describing the potential dependent opening and closing of ion channels formed by syringomycin E.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Kaulin
authors.forename: L V
authors.initials: LV
authors.lastname: Shchagina
mesh_terms: D004573:Electrolytes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry / Q000502:physiology*; D007700:Kinetics; D008051:Lipid Bilayers; D010456:Peptides, Cyclic / Q000737:chemistry*; D012996:Solutions; D014867:Water / Q000737:chemistry
chemical_list: D004573:Electrolytes; D007473:Ion Channels; D008051:Lipid Bilayers; D010456:Peptides, Cyclic; D012996:Solutions; D014867:Water; C105246:syringomycin E