Abnormal accumulation of G2/M-phase cells from LEC strain rats after X-irradiation at S phase.
The effect of X-irradiation on the progression of the cell cycle in cell lines from LEC and WKAH rats was investigated by a flow cytometer. When the cells were exposed to 5 Gy of X-rays at S phase, the proportion of S-phase cells in both cell populations decreased with incubation time and that of G2/M-phase cells was approximately 80% at 6 hr post-irradiation. At 12 hr post-irradiation, approximately 45% of the WKAH rat cells appeared in the G1 phase. However, 80-90% of LEC rat cells remained in the G2/M phase and less than 5% in the G1 phase during 6-12 hr post-irradiation. Thus, the LEC rat cells irradiated at S phase remained in the G2/M phase for at least 6 hr longer than did the WKAH rat cells.
Hayashi
M
M
Department of Veterinary Radiology, Faculty of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Kuzumi
T
T
Arai
S
S
Okui
T
T
D000818:Animals; D016195:G2 Phase; D008938:Mitosis; D051381:Rats; D020332:Rats, Inbred LEC; D016196:S Phase / Q000528:radiation effects*; D014916:Whole-Body Irradiation