title: A negative regulatory element in a prespore-specific promoter of dictyostelium discoideum(1).
abstract: We previously isolated several 'promoter-trap' transformants in which insertion of a promoterless beta-galactosidase gene into the genome caused expression of beta-galactosidase in specific cell types. The upstream flanking region was rescued from one transformant specifically expressing beta-galactosidase in prespore cells. We sequenced the promoter of the gene that is fused in-frame with lacZ and characterised a negative element that inhibits expression in pstO cells (a subtype of prestalk cells). Gel-retardation assays show that a developmentally regulated factor(s) recognises and binds to this element.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Hsu
authors.forename: W
authors.initials: W
authors.lastname: Chang
authors.forename: P C
authors.initials: PC
authors.lastname: Newell
authors.forename: J D
authors.initials: JD
authors.lastname: Gross
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D004023:Dictyostelium / Q000235:genetics* / Q000502:physiology; D015723:Gene Library; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013170:Spores / Q000235:genetics
chemical_list: D018076:DNA, Complementary