Serotonin modulation of catalepsy induced by N(G)-nitro-L-arginine in mice.
N(G)-(Nitro-L-arginine (L-NOARG), an inhibitor of nitric oxide synthase, induces catalepsy in mice. The objective of the present work was to investigate if serotonergic drugs are able to modulate this effect. Results showed that the cataleptogenic effect of L-NOARG (40 mg/kg) in male albino-Swiss mice was enhanced by pre-treatment with (+)-N-tert-butyl-3-(4-[2-methoxyphenyl]piperazin-1-yl)-2-phenylpro panamide ((+)-WAY-100135, 5 or 10 mg/kg), a 5-HT1A-selective receptor antagonist, and by ketanserin (5 or 10 mg/kg), a 5-HT2A receptor and alpha1-adrenoceptor antagonist. Prazosin (3 or 5 mg/kg), an alpha1-adrenoceptor antagonist, and endo-N-(8-methyl-8-azabicyclo[3.2.1]oct-3yl)-2,3-dihydro-3,3-dimet hyl-indole-1-carboxamide HCl (BRL-46470A, 0.05 or 0.5 mg/kg), a 5-HT3 receptor antagonist, did not interfere with L-NOARG-induced catalepsy. Ritanserin (3 or 10 mg/kg), a 5-HT2A and 5-HT2C receptor antagonist, tended to enhance the effect of L-NOARG. These results confirm that interference with the formation of nitric oxide induces catalepsy in mice, and suggest that this effect is modulated by 5-HT1A and 5-HT2A receptors.
Nucci-da-Silva
L P
LP
Department of Physiology, School of Odontology, FORP, Campus USP, Ribeirão Preto, SP, Brazil.
Guimarães
F S
FS
Del Bel
E A
EA
D000317:Adrenergic alpha-Antagonists / Q000633:toxicity; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000633:toxicity*; D002375:Catalepsy / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000633:toxicity*; D007211:Indoles / Q000633:toxicity*; D007650:Ketanserin / Q000633:toxicity; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D019335:Nitroarginine / Q000633:toxicity*; D010879:Piperazines / Q000633:toxicity; D011224:Prazosin / Q000633:toxicity; D016713:Ritanserin / Q000633:toxicity; D012702:Serotonin Antagonists / Q000633:toxicity*; D013997:Time Factors
D000317:Adrenergic alpha-Antagonists; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004791:Enzyme Inhibitors; D007211:Indoles; D010879:Piperazines; D012702:Serotonin Antagonists; C075685:BRL 46470; C081293:WAY 100135; D016713:Ritanserin; D019335:Nitroarginine; D009569:Nitric Oxide; D007650:Ketanserin; D019001:Nitric Oxide Synthase; D011224:Prazosin