Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives.
Multicolour genomic in situ hybridization was carried out in wheat-rye hybrids and in a wheat-rye translocation line. Different hybridization conditions and mixture compositions were used, and A, B and D genomes of hexaploid wheat as well as the R genome of rye were distinguished simultaneously in somatic cells. Combination of genomic and rDNA probes in multicolour in situ hybridization was also performed to identify chromosomes within a specific genome.
Sánchez-Morán
E
E
Unidad de Genética, Departamento de Biotecnología, ETSI Agrónomos, Universidad Politécnica de Madrid, 28040-Madrid, Spain.
Benavente
E
E
Orellana
J
J
D018744:DNA, Plant; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D012434:Secale / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant