Optimizing culture of Chlamydia pneumoniae by using multiple centrifugations.
Three methods for the recovery of Chlamydia pneumoniae from spiked nasopharyngeal and blood specimens, including extended culture and additional centrifugations, were compared. Additional centrifugations and a 7-day culture time resulted in a 500- to 5, 000-fold increase in the number of detectable inclusion-forming units.
Pruckler
J M
JM
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jmp5@cdc.gov
Masse
N
N
Stevens
V A
VA
Gang
L
L
Yang
Y
Y
Zell
E R
ER
Dowell
S F
SF
Fields
B S
BS
D002478:Cells, Cultured; D002498:Centrifugation; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology