title: Immunomagnetic recovery of human neutrophil defensins from the human gingival crevice.
abstract: The human neutrophilic protein defensins are cationic, antimicrobial, cytotoxic, corticostatic chemotactic, opsonic peptides found in azurophilic granules and constitute about 5% of the total protein in human neutrophils. In the present study, defensins were recovered from the human gingival crevice using paramagnetic microspheres (beads), coated with anti-defensin antibodies. The bead-bound defensins were assayed using an enzyme-linked immunosorbent assay developed in this laboratory. Twenty sites were sampled; defensin was found in 100% of the sites and ranged in amount from 270-2000 ng/site. The large local concentrations of defensins, estimated in the mg/ml range, may have major effects on the microbiology of the gingival crevice.
authors.affiliation: Department of BioStructure and Function, University of Connecticut School of Dental Medicine, Farmington 06030, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: McKay
authors.forename: E
authors.initials: E
authors.lastname: Olson
authors.forename: M A
authors.initials: MA
authors.lastname: Hesla
authors.forename: A
authors.initials: A
authors.lastname: Panyutich
authors.forename: T
authors.initials: T
authors.lastname: Ganz
authors.forename: S
authors.initials: S
authors.lastname: Perkins
authors.forename: E F
authors.initials: EF
authors.lastname: Rossomando
mesh_terms: D000328:Adult; D023082:Defensins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005881:Gingiva / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008297:Male; D009504:Neutrophils / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D023082:Defensins; D011506:Proteins