title: Evaluation of kits for the detection of fibrin(ogen) degradation products in dogs.
abstract: The sensitivities and specificities of 3 commercial serum fibrin(ogen) degradation product (FDP) kits and 1 plasma FDP kit for the detection of FDPs in dogs were determined. Blood was collected for measurement of serum and plasma FDP concentrations from 30 healthy dogs and from 20 dogs that fulfilled clinical and laboratory criteria for disseminated intravascular coagulation. To determine the effect of hemolysis on FDP results, blood was collected simultaneously into Bothrops atrox venom-based and thrombin-based serum collection tubes for measurement of FDPs using a single serum FDP kit. The sensitivity (80-95%) and specificity (90-100%) for a positive or negative FDP result, regardless of concentration, was similar for all kits. Kits yielded discordant results in individual dogs and FDP concentrations obtained from 1 serum FDP kit were consistently higher than those from the other kits. Serum prepared from venom-based collection tubes was significantly more hemolyzed than serum prepared from thrombin-based collection tubes or citrated plasma. Hemolysis did not affect the FDP results. On the basis of these results, we conclude that commercial latex agglutination kits for detection of FDPs in serum and plasma samples from human patients are valid for use in dogs. The plasma FDP assay is a viable alternative to currently used serum FDP assays and has the advantage of using a single (citrated plasma) sample for measuring coagulation parameters and FDP concentration.
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850-6401, USA. ts23@cornell.edu
authors.forename: T
authors.initials: T
authors.lastname: Stokol
authors.forename: M
authors.initials: M
authors.lastname: Brooks
authors.forename: H
authors.initials: H
authors.lastname: Erb
authors.forename: G E
authors.initials: GE
authors.lastname: Mauldin
mesh_terms: D000818:Animals; D012103:Batroxobin; D004211:Disseminated Intravascular Coagulation / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D005342:Fibrinolysis; D005343:Fibrinolytic Agents; D006461:Hemolysis; D007841:Latex Fixation Tests / Q000295:instrumentation / Q000662:veterinary; D008297:Male; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation / Q000662:veterinary
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D005343:Fibrinolytic Agents; D011933:Reagent Kits, Diagnostic; D012103:Batroxobin