Do nuclear condensation or fragmentation and DNA fragmentation reflect the mode of neuronal death?
It is generally believed that nuclear condensation and fragmentation as well as DNA fragmentation reflect the events related to the neuronal apoptosis. Our report demonstrates that severe oxygen-glucose deprivation (OGD) induced condensation and fragmentation of nuclear chromatin of neurones in primary cultures of cerebellar granule cells without intemucleosomal DNA fragmentation. DNA fragmentation detected by TUNEL assay was seen only after mild OGD or after addition of colchicine but not after severe OGD. Thus, at least in primary cerebellar granule cell cultures, the chromatin condensation and fragmentation cannot be considered as a hallmark of apoptosis but rather reflect the neuronal death despite of its form.
Kaasik
A
A
Department of Pharmacology, University of Tartu, Estonia.
Vassiljev
V
V
Poldoja
E
E
Kalda
A
A
Zharkovsky
A
A
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D002531:Cerebellum / Q000166:cytology; D003078:Colchicine / Q000494:pharmacology; D053938:DNA Fragmentation; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D010100:Oxygen / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D016291:Dizocilpine Maleate; D005947:Glucose; D010100:Oxygen; D003078:Colchicine