Expression of IgH chain genes in antibody response to ragweed and purified Amb a 1: evidence for differential regulation of isotype production.
A survey of the work with Ig response to allergens carried out previously reveals an allergen-specific response both by IgE and all of IgG subclasses. Response of non-sensitive people is characterized by the appearance of a variety of the IgG subclasses. We have reexamined ragweed and Amb a 1 specific Ig response in 54 nonsensitive and 147 atopic or atopic-allergic people using a new inverse sandwich immunoassay allowing discrimination based on antibody affinity. We show that non-sensitive people present no, 0 out of 54, Ig response with affinities higher than Ka 10(7) M(-1). The subpopulation of 66 atopics who never have experienced desensitization responds vigorously and solely (56 out of 66) with genes of the sequence gamma2-alpha2. Only ten showed an additional weak response from gamma1-alpha1. This suggests a possible association between the atopic state and selective activation of part of the gene sequence.
Allauzen
S
S
The Asthma and Allergy Center, Department of Medicine, University of Minnesota Medical School, Minneapolis, USA.
Blocki
F
F
Pierson-Mullany
L
L
Jackola
D
D
Dalan
D
D
Larue
C
C
Blumenthal
M
M
Rosenberg
A
A
D000485:Allergens; D000918:Antibody Specificity; D052179:Antigens, Plant; D019659:Asteraceae / Q000276:immunology; D001249:Asthma / Q000276:immunology; D003429:Cross Reactions; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000628:therapy; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000628:therapy; D007118:Immunoassay; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis*; D007167:Immunotherapy; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D012220:Rhinitis / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; C026967:Amb a I protein, Ambrosia artemisiifolia; D052179:Antigens, Plant; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D010940:Plant Proteins; D007073:Immunoglobulin E