Autosomal trisomy 20 (61,XX,+20) in a malformed bovine fetus.
A 240-day-gestation female bovine fetus with severe anasarca, palatoschisis, cheiloschisis, mild cranioschisis, and a flattened facies was collected at a slaughterhouse, and a fibroblast line was established from the fetal skin. Chromosome preparations were Q-banded, and chromosome counts were taken that indicated the presence of 61 chromosomes in cells of the fetus (the normal diploid number for domestic cattle is 60). Q-band karyotypes were constructed, and Q-band analysis revealed the presence of three copies of chromosome 20. Trisomy 20 (61,XX,+20) was confirmed through the use of two-color fluorescence in situ hybridization of bovine bacterial artificial chromosome clones that were specific to chromosome 20 and the X chromosome.
Gallagher
D S
DS
Lewis
B C
BC
De Donato
M
M
Davis
S K
SK
Taylor
J F
JF
Edwards
J F
JF
D000818:Animals; D006690:Bisbenzimidazole / Q000737:chemistry; D002417:Cattle / Q000002:abnormalities* / Q000196:embryology / Q000235:genetics; D005260:Female; D005333:Fetus / Q000002:abnormalities*; D005456:Fluorescent Dyes / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007621:Karyotyping / Q000662:veterinary; D014314:Trisomy / Q000235:genetics / Q000473:pathology*
D005456:Fluorescent Dyes; D006690:Bisbenzimidazole