title: Validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis.
abstract: Sera from Canadian pigs (brucellosis free, n = 14037) and sera from pigs infected with Brucella suis (n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. The results were analysed and assay sensitivity and specificity estimates were calculated. The sensitivity and specificity of the tests were as follows: the buffered antigen plate agglutination test, 77.1 and 96.9%; the complement fixation test (considering anticomplementary sera as negative), 93.3 and 95.5%; the complement fixation test (considering anticomplementary sera as positive), 58.1 and 99.9%; the indirect enzyme immunoassay, 94.0 and 97.9%; the competitive enzyme immunoassay, 90.8 and 96.6%; and the fluorescence polarization assay, 93.5 and 97.2%; respectively. It was concluded that the fluorescence polarization assay was a valuable asset to the diagnosis of porcine brucellosis because of its accuracy, ease of performance and relative cost.
authors.affiliation: Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ont. nielsenk@em.agr.ca
authors.forename: K
authors.initials: K
authors.lastname: Nielsen
authors.forename: D
authors.initials: D
authors.lastname: Gall
authors.forename: P
authors.initials: P
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Vigliocco
authors.forename: B
authors.initials: B
authors.lastname: Perez
authors.forename: L
authors.initials: L
authors.lastname: Samartino
authors.forename: P
authors.initials: P
authors.lastname: Nicoletti
authors.forename: A
authors.initials: A
authors.lastname: Dajer
authors.forename: P
authors.initials: P
authors.lastname: Elzer
authors.forename: F
authors.initials: F
authors.lastname: Enright
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016231:Fluorescence Polarization Immunoassay / Q000662:veterinary*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial