title: 4,4'-diapophytoene desaturase: catalytic properties of an enzyme from the C(30) carotenoid pathway of Staphylococcus aureus.
abstract: Staphylococcus aureus synthesizes C(30) carotenoids. Their formation involves the introduction of three double bonds, which is catalyzed by a single enzyme. This enzyme, 4,4'-diapophytoene desaturase from S. aureus, was overexpressed in Escherichia coli and purified in one step by affinity chromatography, and then the protein was characterized with respect to substrate specificity, cofactor requirement, and oligomerization.
authors.affiliation: Biosynthesis Group, Botanisches Institut, J. W. Goethe Universität Frankfurt, 60054 Frankfurt, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Raisig
authors.forename: G
authors.initials: G
authors.lastname: Sandmann
mesh_terms: D001426:Bacterial Proteins; D002338:Carotenoids / Q000096:biosynthesis*; D010088:Oxidoreductases / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D002338:Carotenoids; C091055:CrtN protein, Staphylococcus aureus; D010088:Oxidoreductases