The effects of two stereoisomers of N-acetylcysteine on photochemical damage by UVA and blue light in rat retina.
High doses of light can cause damage to the retina, e.g. during intraocular surgery. Previously, thiols have been demonstrated to protect against retinal damage in various damage models. Such protection is very promising for clinical practice. Retinal light damage can be caused by a relatively short exposure to high irradiance levels. These conditions occur during intraocular surgery. In the current study we therefore investigated whether the thiol N-acetylcysteine protects against retinal light damage under high irradiance conditions in the rat retina. Two stereoisomers of this thiol were tested for protection against two spectrally defined types of retinal light damage. Shortly after administration N-acetyl-L-cysteine in doses of 270-1000 mg/kg intraperitoneally protected against 380 nm (UVA) light but not against 470 nm (blue) light. Two hours after injection the protection had diminished. We observed no protection by the stereoisomer N-acetyl-D-cysteine. From this study we conclude that N-acetyl-L-cysteine protects stereospecifically against retinal damage in the UV but not in the visible part of the spectrum. This limits the possible applications.
Busch
E M
EM
F. C. Donders Institute of Ophthalmology, Utrecht, The Netherlands.
Gorgels
T G
TG
Roberts
J E
JE
van Norren
D
D
D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008027:Light; D010777:Photochemistry; D051381:Rats; D012160:Retina / Q000187:drug effects* / Q000293:injuries / Q000528:radiation effects*; D012164:Retinal Diseases / Q000209:etiology / Q000517:prevention & control*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D016166:Free Radical Scavengers; D000111:Acetylcysteine