Gene expression of P2X-receptors in the developing inner ear of the rat.
Reverse transcription-polymerase chain reaction (RT-PCR) was used to characterize the expression of P2X receptor subunits (P2X1-P2X7) in different inner ear tissues. The present study revealed the presence of P2X2, P2X3, P2X4 and P2X7-mRNA in rat organ of Corti, vestibular organ and spiral ganglion at different postnatal developmental stages (PD1-PD16), with slight differences in the onset of expression. Expression of P2X1, P2X5 and P2X6-mRNA was not detectable in the inner ear tissues. In addition, single cell RT-PCR experiments with outer hair cells (OHC) revealed the expression of either the P2X2 or the P2X2-2 splice variant or coexpression of both isoforms in individual cells. Our data suggest that extracellular adenosine-5'-triphosphate (ATP) may play an important role in signal transduction in the inner ear.
Brändle
U
U
Department of Otolaryngology, University of Tuebingen, Germany.
Zenner
H P
HP
Ruppersberg
J P
JP
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D004274:DNA, Recombinant; D007758:Ear, Inner / Q000502:physiology*; D015870:Gene Expression; D014644:Genetic Variation; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism; D009925:Organ of Corti / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000235:genetics*; D058476:Receptors, Purinergic P2X2; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013136:Spiral Ganglion / Q000378:metabolism; D014722:Vestibule, Labyrinth / Q000378:metabolism
D004274:DNA, Recombinant; C546403:P2rx2 protein, rat; D020033:Protein Isoforms; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2