title: Cloning and sequencing of the gene encoding universal stress protein from Escherichia coli O157:H7 isolated from Jack-in-a-Box outbreak.
abstract: A gene encoding the universal stress protein (uspA) was cloned from Escherichia coli O157:H7 isolated from a Jack-in-a-Box outbreak. The nucleotide sequence was determined and the amino acid sequence predicted. The gene was found to be highly conserved and shared 97% homology with the same gene of E. coli K-12. Within an 800-base pair (bp) region sequenced, a 15-bp mismatch existed between E. coli K-12 and O157:H7, of which 11 base pairs were internal to the structural gene of uspA. However, only an area with a 4-bp mismatch caused an amino acid substitution, changing an alanine to an arginine at position 140.
authors.affiliation: Center for Food Safety and Quality Enhancement, University of Georgia, Griffin 30223-1797, USA. jchen@cfsqe.griffin.peachnet.edu
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: M W
authors.initials: MW
authors.lastname: Griffiths
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D019453:Escherichia coli O157 / Q000737:chemistry / Q000235:genetics*; D005516:Food Microbiology; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C078564:universal stress protein A, Bacteria