Rat embryonic myoblasts are restricted to forming primary fibres while later myogenic populations are pluripotent.
Three populations of myoblasts, embryonic, foetal and adult, appear sequentially during myogenesis. The present study uses retroviruses to mark myoblasts clones in vivo from these populations. Myoblasts labelled at E15 (embryonic) contributed to primary fibres only. The majority of marked primary fibres were slow but a small number of clones contained marked primaries which were no longer slow at E19. Myoblasts labelled at E17 (foetal) fused with both primary and secondary fibres and most clones contained both fast and slow fibres. Similarly, adult myoblasts marked at P0 fused with all fibre types. These results indicate that embryonic myoblasts are restricted to producing only primary fibres which are initially slow but which can convert to being fast. Clones of foetal and adult myoblasts fuse with both primary and secondary fibres which may be either fast or slow.
Dunglison
G F
GF
School of Biomedical Sciences, University of Nottingham, The Medical School, Queens Medical Centre, Nottingham, UK.
Scotting
P J
PJ
Wigmore
P M
PM
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D019070:Cell Lineage; D002999:Clone Cells / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000196:embryology*; D051381:Rats; D012190:Retroviridae / Q000378:metabolism; D013997:Time Factors