Crystallization and preliminary X-ray crystallographic studies of the D2 region of the skeletal muscle ryanodine receptor.
The N-terminal portion (amino acids 1303-1367) of the type 1 ryanodine receptor D2 region is thought to be critical for excitation-contraction coupling in skeletal muscle. A segment of the D2 region (amino acids 1317-1355) was expressed as a glutathione S-transferase fusion protein (GST-D2) and then crystallized at room temperature using ammonium sulfate as precipitant. Using a newly developed cryo-soaking method, complete native data sets were measured to a resolution of 2.2 A using synchrotron radiation. The crystal was found to be hexagonal, belonging to space group P6(3)22, with unit-cell parameters a = b = 116.1, c = 77.9 A.
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Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, South Korea.
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D000818:Animals; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D019837:Ryanodine Receptor Calcium Release Channel / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012967:Sodium Dodecyl Sulfate
D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D012967:Sodium Dodecyl Sulfate; D005982:Glutathione Transferase