title: Gastric ulcers in SCID mice induced by Helicobacter pylori infection after transplanting lymphocytes from patients with gastric lymphoma.
abstract: OBJECTIVE
  Several studies have indicated that host factors are important in Helicobacter pylori-induced gastroduodenal diseases. We examined the pathological role of host immune responses in H. pylori infection by reconstituting components of the human immune system into severe combined immunodeficient (SCID) mice by transplantation of peripheral blood mononuclear cells (PBMCs) from H. pylori-infected patients.
  
  
  METHODS
  PBMCs obtained from patients with mucosa-associated lymphoid tissue (MALT) lymphoma were injected intraperitoneally into SCID mice, designated MALToma-hu-SCID mice. One month after transplantation, H. pylori was administered orally to the mice. The mice were killed and examined for pathological changes and immunologic features.
  
  
  RESULTS
  Human lymphocytes were detected in hu-SCID mice, and T- and B-cell functions were preserved for 1 month. Administration of H. pylori led to gastric ulcers with bleeding in the MALToma-hu-SCID mice. The gastric mucosa of control mice injected with Escherichia coli or transplanted with PBMCs from patients with peptic ulcers or gastritis or from healthy volunteers showed no pathological changes.
  
  
  CONCLUSIONS
  Host immune responses against H. pylori appear to be involved in the development of gastric ulcers in patients who have MALT lymphoma.
authors.affiliation: Department of Bacteriology, Okayama University Medical School, Okayama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yokota
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawahara
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Akagi
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Oguma
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis; D002455:Cell Division; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000276:immunology / Q000502:physiology; D006801:Humans; D017710:Lymphocyte Transfusion; D018442:Lymphoma, B-Cell, Marginal Zone / Q000097:blood* / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D009000:Monocytes / Q000473:pathology; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D013270:Stomach / Q000276:immunology / Q000473:pathology; D013274:Stomach Neoplasms / Q000097:blood* / Q000276:immunology; D013276:Stomach Ulcer / Q000382:microbiology* / Q000473:pathology
chemical_list: D000907:Antibodies, Bacterial; D001323:Autoantibodies