Chromosome segregation and cell division defects in recBC sbcBC ruvC mutants of Escherichia coli.
The RuvC protein is important for DNA recombination and repair in Escherichia coli. The present work shows that a ruvC null mutation introduced into a recBC sbcBC background causes severe defects in chromosome segregation and cell division. Both defects were found to result from abortive recombination initiated by the RecA protein.
Zahradka
D
D
Department of Molecular Genetics, Ruer Boskovic Institute, 10001 Zagreb, Croatia. zahradka@rudjer.irb.hr
Vlahović
K
K
Petranović
M
M
Petranović
D
D
D001426:Bacterial Proteins / Q000235:genetics*; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis; D004706:Endodeoxyribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C069806:ruvC protein, E coli; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli