Experience with a mouse intranasal test for the predictive identification of respiratory sensitization potential of proteins.
The predictive identification of respiratory allergenic potential is an important primary step in the safety evaluation of (novel) proteins, such as the enzymes used in a range of consumer laundry products. In the past this has been achieved by assessing the relative ability of proteins to give rise to the formation of anaphylactic antibody in the guinea pig. Recently, an alternative model has been proposed which assesses the formation of specific IgG1 antibody in a mouse intranasal test (MINT), the assumption being that specific IgG1 antibody is a surrogate for anaphylactic antibody in the mouse. This procedure has undergone successful initial intralaboratory and interlaboratory assessment. In the present work, the MINT has been evaluated in a more thorough intralaboratory study using eight enzymes plus ovalbumin. While the data generated with a reference enzyme protein, Alcalase, showed good reproducibility, results with the remaining eight proteins led to estimates of their relative antigenic or sensitization potential several of which were at variance from those derived from the guinea pig/ human experience. In consequence, it is concluded that the MINT requires substantial further investigation before it can be adopted as a model for the assessment of the relative ability of proteins to behave as respiratory allergens.
Blaikie
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Unilever Safety and Environmental Assurance Centre, Sharnbrook, Bedford, UK.
Basketter
D A
DA
D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D000906:Antibodies / Q000097:blood; D003902:Detergents / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000633:toxicity; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007159:Immunologic Tests; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000633:toxicity; D011506:Proteins / Q000008:administration & dosage / Q000633:toxicity*; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000139:chemically induced*; D013381:Subtilisins / Q000008:administration & dosage
D000485:Allergens; D000906:Antibodies; D003902:Detergents; D004798:Enzymes; D007074:Immunoglobulin G; D011506:Proteins; D010047:Ovalbumin; D013381:Subtilisins