Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates.
Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoxide (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation.
Nam
Y S
YS
Department of Biological Sciences and Technology, Yusong-gu, Taejon, Korea.
Park
T G
TG
D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002942:Circular Dichroism; D003692:Delayed-Action Preparations; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D008863:Microspheres; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D012997:Solvents; D014835:Volatilization
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011108:Polymers; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase; D004121:Dimethyl Sulfoxide