Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.
We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.
Scarpellini
P
P
Infectious Diseases Division, San Raffaele Scientific Institute, University of Milan, Milan, Italy. scarpellini.paolo@hsr.it
Braglia
S
S
Carrera
P
P
Cedri
M
M
Cichero
P
P
Colombo
A
A
Crucianelli
R
R
Gori
A
A
Ferrari
M
M
Lazzarin
A
A
D000328:Adult; D000368:Aged; D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology*
D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial; D010940:Plant Proteins; C101305:RpoB protein, Oryza sativa; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin