Using RT-PCR and bDNA assays to measure non-clade B HIV-1 subtype RNA.
The performance of the new version of RT-PCR assay (Amplicor HIV-1 Monitor v1.5) was assessed. The quantification of non-B subtype HIV-1 plasma RNA (30A, 1C, 1D, 3E, 2F, 3G) obtained using Monitor v1.5 was compared to the former version of this assay (Monitor v1.0) and to the Quantiplex v2.0 bDNA assay. The new primers used in Monitor v1.5 were similar to the former version in both specificity and sensitivity. The new primers corrected the detection and quantification defect observed previously for HIV-1 non-B subtypes and gave slightly higher RNA concentrations than those measured using the bDNA assay (+0.39 log copies/ml).
Pasquier
C
C
Laboratoire de Virologie, Centre Hospitalier Universitaire Purpan, Toulouse, France. cpasquie@cict.fr
Sandres
K
K
Salama
G
G
Puel
J
J
Izopet
J
J
D005260:Female; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D011247:Pregnancy; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic