Epstein-Barr virus-encoded RK-BARF0 protein expression.
The cellular localization of the Epstein-Barr virus-encoded RK-BARF0 protein was analyzed by fluorescence microscopy and immunoblotting. The recombinant RK-BARF0 protein was found to be tightly bound to nuclear structures, whereas 16- to 20-kDa RK-BARF0 derivatives, generated by differential splicing of the RK-BARF0 transcript, were present throughout the cell. Moreover, a previously generated anti-RK-BARF0 rabbit serum was found to cross-react with cellular proteins, showing that the previously identified 30- to 35-kDa membrane-associated proteins do not represent RK-BARF0.
Kienzle
N
N
EBV Unit, The Queensland Institute of Medical Research and University of Queensland Joint Oncology Program, Brisbane, Australia. norbertK@qimr.edu.au
Buck
M
M
Greco
S
S
Krauer
K
K
Sculley
T B
TB
D000818:Animals; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics*
C121639:RK-BARF0 protein, Human herpesvirus 4; D011994:Recombinant Proteins; D014764:Viral Proteins