title: Serological investigation of Chlamydia trachomatis heat shock protein 10.
abstract: The humoral immune response to Chlamydia trachomatis 10-kDa heat shock protein (Chsp10) in populations of Russian and French origin was studied by using a recombinant Chsp10 enzyme-linked immunosorbent assay. A physiological but not a serological correlation of Chsp10 exposure with Chsp60 exposure was observed in the Russian population. In the French population studied, there was a significant association between detection of anti-r-Chsp10 immunoglobulin G (IgG) antibodies and chronic genital tract infections. Chsp10 residues 50 to 67 were found to contain an immunodominant although not universal B epitope. Cross-reactions with Chlamydia pneumoniae or Escherichia coli GroES protein are limited but may occur. Our study suggests that detection of anti-Chsp10 IgG antibodies is associated with chronicity of C. trachomatis genital tract infection and does not parallel that of anti-Chsp60 IgG antibodies.
authors.affiliation: Biobanque de Picardie, University Hospital, Amiens, France.
authors.forename: F
authors.initials: F
authors.lastname: Betsou
authors.forename: J M
authors.initials: JM
authors.lastname: Sueur
authors.forename: J
authors.initials: J
authors.lastname: Orfila
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000032:analysis*; D018835:Chaperonin 10 / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D018835:Chaperonin 10; D018834:Chaperonin 60