title: Inflammatory responses to amyloid fibrils.
abstract: Our laboratory has routinely used the methodologies described here to characterize the effects of fibrillar A beta and amylin on cytokine synthesis and secretion by LPS-differentiated THP-1 cells. Because LPS-treated THP-1 cells resemble macrophage and microglia, this assay system represents an in vitro model of the potential interactions between A beta-containing senile plaques and microglia in the AD brain. As such, these methodologies should prove useful in the identification of compounds that inhibit this A beta-induced inflammatory response.
authors.affiliation: Gliatech Inc., Cleveland, Ohio 44122, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Yates
authors.forename: J
authors.initials: J
authors.lastname: Kocsis-Angle
authors.forename: P
authors.initials: P
authors.lastname: Embury
authors.forename: K R
authors.initials: KR
authors.lastname: Brunden
mesh_terms: D000682:Amyloid / Q000235:genetics / Q000276:immunology*; D016229:Amyloid beta-Peptides / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D058228:Islet Amyloid Polypeptide; D009000:Monocytes / Q000276:immunology*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D007375:Interleukin-1; D058228:Islet Amyloid Polypeptide; D011994:Recombinant Proteins