title: Effect of estradiol metabolites on prostacyclin synthesis in human endothelial cell cultures.
abstract: Estradiol can stimulate prostacyclin production in the vessel wall, thereby eliciting vasodilatation. In the present work the effect of the estradiol metabolites estrone, 2-methoxyestrone, 2-methoxyestradiol, and 16alpha-hydroxyestrone were investigated to find out if they are also able to stimulate prostacyclin synthesis. All metabolites triggered an increase of prostacyclin synthesis in human endothelial cells starting at a concentration of 10(-9) M. The parent substance, 17beta-estradiol, accomplished this effect only starting at a concentration of 10(-8) M. These results indicate that estradiol metabolites may take part in the estradiol-induced vasodilatation in vivo.
authors.affiliation: Department of Obstetrics and Gynecology, University Tuebingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Seeger
authors.forename: A O
authors.initials: AO
authors.lastname: Mueck
authors.forename: T H
authors.initials: TH
authors.lastname: Lippert
mesh_terms: D000077584:2-Methoxyestradiol; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D004970:Estrone / Q000494:pharmacology; D006801:Humans; D006894:Hydroxyestrones / Q000494:pharmacology
chemical_list: D006894:Hydroxyestrones; C026702:16-hydroxyestrone; D004970:Estrone; D004958:Estradiol; D000077584:2-Methoxyestradiol; D011464:Epoprostenol; C003655:2-methoxyestrone