Upregulation of type I interferon receptor by IFN-gamma.
Type I interferon (IFN) receptor has a multichain structure composed of at least two distinct subunits, IFNAR-1 and IFNAR-2. In the present study, we demonstrated that IFN-gamma induced the expression of mRNA for IFNAR-1 and IFNAR-2 in a human hepatoma cell line, HepG2 cells. The induction was dose and time dependent. Because of this result, we examined the effect of combined treatment with type I IFN and IFN-gamma. The intracellular 2-5A-synthetase activity induced by combined treatment was significantly higher than that by type I IFN alone. This study suggests that combined treatment with type I IFN and IFN-gamma may be more effective than that of type I IFN alone and that the upregulation of type I IFN receptor may be one of the reasons. Our findings may have some relevance to the clinical use of IFN.
Mizukoshi
E
E
First Department of Internal Medicine, Kanazawa University School of Medicine, Ishikawa, Japan.
Kaneko
S
S
Yanagi
M
M
Ohno
H
H
Matsushita
E
E
Kobayashi
K
K
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D006801:Humans; D016898:Interferon-alpha; D007371:Interferon-gamma / Q000494:pharmacology*; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000187:drug effects*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D016898:Interferon-alpha; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D053633:Receptor, Interferon alpha-beta; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase