Establishment of an estrogen responsive rat pituitary cell sub-line MtT/E-2.
A rat pituitary tumor sub-line MtT/E-2 was established from a rat pituitary tumor cell line MtT/E. Its growth was found to depend on the presence of estradiol (E2) in culture media at 10(-13)-10(-9) M, whereas the original cell line MtT/E proliferated autonomously. The recently discovered PTTG (pituitary tumor transforming gene) is highly expressed in this cell line, although not regulated by E2. On the other hand, E2 induced c-myc and cyclin D1 proteins in MtT/E-2, which contains a lot (220+/-18 fmol/mg protein) of estrogen receptor demonstrated by RT-PCR analysis to be predominantly alpha type. MtT/E-2 secretes growth hormone which is, interestingly, regulated by retinoic acid and dexamethasone rather than thyroid hormones.
Fujimoto
N
N
Department of Cancer Research, Research Institute for Radiation Biology and Medicine, Hiroshima University.
Maruyama
S
S
Ito
A
A
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D019938:Cyclin D1 / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics; D013006:Growth Hormone / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics; D009368:Neoplasm Transplantation; D015513:Oncogene Proteins / Q000235:genetics; D010911:Pituitary Neoplasms / Q000473:pathology*; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064249:Securin; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D009363:Neoplasm Proteins; D015513:Oncogene Proteins; D011960:Receptors, Estrogen; D064249:Securin; D019938:Cyclin D1; D004958:Estradiol; D014212:Tretinoin; D003907:Dexamethasone; D013006:Growth Hormone