The cassettes and 3' conserved segment of an integron from Klebsiella oxytoca plasmid pACM1.
pACM1 is a conjugative multiresistance plasmid from Klebsiella oxytoca that encodes SHV-5 extended-spectrum beta-lactamase (ESBL) and has two integrons. The first is a type I (sul type); the second, detected by hybridization with an intI gene probe, has been putatively identified as a defective type I integron. The cassette region of the first integron has now been fully sequenced and contains three aminoglycoside resistance determinants (aac(6')-Ib, aac(3)-Ia, and ant(3")-Ia) and two open reading frames of unknown function. In addition, sequencing of a region downstream from the qacEDelta1-sulI-ORF 5 gene cluster of the first integron revealed a copy of insertion sequence IS6100 flanked by inverted copies of sequence from the 11.2-kb insert (In2) of Tn21. This arrangement is similar to that found in In4 of Tn1696. The coincidence of an ESBL gene and mobile elements on a conjugative plasmid has potential implications for the spread of ESBL-mediated drug resistance, though evidence of bla((SHV-5)) movement mediated by these elements has not been found.
Preston
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KE
Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, Albany, New York 12208, USA.
Radomski
C C
CC
Venezia
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RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D006801:Humans; D007709:Klebsiella / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases