title: Developing dendritic cell polynucleotide vaccination for prostate cancer immunotherapy.
abstract: Immunotherapy has been successfully used to treat some human malignancies, principally melanoma and renal cell carcinoma. Genetic-based cancer immunotherapies were proposed which prime T lymphocyte recognition of unique neo-antigens arising from specific mutations. Genetic immunization (polynucleotide vaccination, DNA vaccines) is a process whereby gene therapy methods are used to create vaccines and immunotherapies. Recent findings indicate that genetic immunization works indirectly via a bone marrow derived cell, probably a type of dendritic antigen presenting cell (APC). Direct targeting of genetic vaccines to these cells may provide an efficient method for stimulating cellular and humoral immune responses to infectious agents and tumor antigens. Initial studies have provided monocytic-derived dendritic cell (DC) isolation and culture techniques, simple methods for delivering genes into these cells, and have also uncovered potential obstacles to effective cancer immunotherapy which may restrict the utility of this paradigm to a subset of patients.
authors.affiliation: University of Maryland School of Medicine, Baltimore 21201-1192, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Berlyn
authors.forename: S
authors.initials: S
authors.lastname: Ponniah
authors.forename: S A
authors.initials: SA
authors.lastname: Stass
authors.forename: J G
authors.initials: JG
authors.lastname: Malone
authors.forename: G
authors.initials: G
authors.lastname: Hamlin-Green
authors.forename: J K
authors.initials: JK
authors.lastname: Lim
authors.forename: M
authors.initials: M
authors.lastname: Cottler-Fox
authors.forename: G
authors.initials: G
authors.lastname: Tricot
authors.forename: R B
authors.initials: RB
authors.lastname: Alexander
authors.forename: D L
authors.initials: DL
authors.lastname: Mann
authors.forename: R W
authors.initials: RW
authors.lastname: Malone
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics; D001709:Biotechnology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007157:Immunologic Surveillance; D007167:Immunotherapy / Q000379:methods; D008297:Male; D009154:Mutation; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D019444:Vaccines, DNA