Analysis of the Corynebacterium glutamicum dapA promoter.
Deletion and mutational analysis of the promoter P-dapA from Corynebacterium glutamicum was performed to identify regions and particular nucleotides important for its function. An extended -10 region and a stretch of six T's at positions -55 to -50 were found to be the most important elements in the promoter function. The results of mutational analysis of P-dapA are consistent with the conclusions of statistical computer-aided analysis of 44 C. glutamicum promoter sequences.
Vasicová
P
P
Institute of Microbiology, Academy of Sciences of the Czech Republic, CZ-14220 Praha 4, Czech Republic.
Pátek
M
M
Nesvera
J
J
Sahm
H
H
Eikmanns
B
B
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000201:enzymology / Q000235:genetics*; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion
D015500:Chloramphenicol O-Acetyltransferase; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase