Catabolism of HDL1 cholesteryl ester in the rat. Effect of ethinyl estradiol treatment.
The present study was performed in control and ethinyl estradiol-treated rats in order to determine the mechanisms involved in the catabolism of HDL1 cholesteryl ester. Ligand blottings on liver membranes showed that purified HDL1, containing about 70% apolipoprotein E and 10% apolipoprotein AI, bind to the LDL receptor (130 kDa) and not to HB2 (100 kDa) or SR-BI (82 kDa), candidate HDL receptors. Immunoblots showed that the treatment increased the hepatic level of the LDL receptor five- to ten-fold, strongly decreased that of SRBI and did not change that of HB2. An in vivo kinetic study showed that the turnover of HDL1 cholesteryl ester is more rapid in treated than control rats. The liver participation (60%) in this clearance was not modified by the treatment. Therefore, it can be concluded that the catabolism of HDL1 cholesteryl ester, in control as in treated rats, is essentially ensured by the uptake of entire particles in the hepatocytes via LDL receptors.
Sérougne
C
C
Laboratoire de physiologie de la nutrition, unité associée université Paris-Sud-XI/Inra, Orsay, France. colette.serougne@ibaic.u-psud.fr
Feurgard
C
C
Hajri
T
T
Champarnaud
G
G
Férézou
J
J
Mathé
D
D
Lutton
C
C
D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001054:Apolipoproteins A / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism; D002352:Carrier Proteins; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D008076:Cholesterol, HDL / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D007700:Kinetics; D008075:Lipoproteins, HDL; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D016601:RNA-Binding Proteins; D051381:Rats; D017208:Rats, Wistar; D011973:Receptors, LDL / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
D001054:Apolipoproteins A; D001057:Apolipoproteins E; D002352:Carrier Proteins; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D016601:RNA-Binding Proteins; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; C031616:high density lipoprotein receptors; C075197:high density lipoprotein binding protein; D004997:Ethinyl Estradiol; D002784:Cholesterol