title: Correlates between hematopoiesis and neuropoiesis: neural stem cells.
abstract: There are many parallels between the neuropoietic and lymphohematopoietic systems. The lymphohematopoietic stem/progenitor cell system has been extensively characterized, but there are still major questions relating to the definitive stem cell assay, the structure of the system (i.e., hierarchical versus cell cycle-based), and the nature of differentiation (i.e., stochastic versus deterministic). Recent data have established the existence of an epidermal growth factor (EGF)-responsive neural stem cell in adult mice. We have studied these neural progenitor/stem cells in fetal (day 15) and 2-day postnatal mice and established a single-cell progenitor assay and a variety of putative uni-, bi-, and tripotential stem cells that form in response to EGF. Neurospheres are the EGF-responsive neural units that grow in liquid culture, and we have found that cells derived from these neurospheres express a wide array of cytokines and their receptors. This will provide a window on the hemopoietic progenitor system analogous to that created by the description of in vitro growth of clonal hematopoietic progenitors.
authors.affiliation: University of Massachusetts Cancer Center, Worcester 01605, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Quesenberry
authors.forename: R
authors.initials: R
authors.lastname: Hulspas
authors.forename: M
authors.initials: M
authors.lastname: Joly
authors.forename: B
authors.initials: B
authors.lastname: Benoit
authors.forename: C
authors.initials: C
authors.lastname: Engstrom
authors.forename: J
authors.initials: J
authors.lastname: Rielly
authors.forename: T
authors.initials: T
authors.lastname: Savarese
authors.forename: L
authors.initials: L
authors.lastname: Pang
authors.forename: L
authors.initials: L
authors.lastname: Recht
authors.forename: A
authors.initials: A
authors.lastname: Ross
authors.forename: G
authors.initials: G
authors.lastname: Stein
authors.forename: M
authors.initials: M
authors.lastname: Stewart
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D009474:Neurons / Q000166:cytology*