Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.
It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small 2hJHC' interactions in the presence of the much larger through-covalent-bond 2JHC' and 3JHC' couplings. The ratio of the observed through-H-bond correlation intensity and the 2JHC' connectivity observed in a reference spectrum indicates 2hJHC' values of ca. 0.4-0.6 Hz, which are only slightly smaller than the corresponding 3hJNC' values. However, for technical reasons, 2hJHC' couplings are more difficult to measure than 3hJNC'.
Cordier
F
F
Institute of Structural Biology, Forschungszentrum Jülich, Jülich, 52425, Germany.
Rogowski
M
M
Grzesiek
S
S
Bax
A
A
D000577:Amides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D003903:Deuterium; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011522:Protons; D014452:Ubiquitins / Q000737:chemistry*
D000577:Amides; D000596:Amino Acids; D011522:Protons; D014452:Ubiquitins; D002244:Carbon; D003903:Deuterium