Early social isolation decreases the expression of calbindin D-28k in rat cerebellar Purkinje cells.
The main objective of this study was to analyze the effects of early social isolation on the expression of calbindin D-28k (CAD-28k) in cerebellar Purkinje cells. Sprague-Dawley rats were reared either in isolation (IC) or socially housed conditions (SC) from postnatal days 18-32. Half of the rats of each group were sacrificed at 32 days of age and the cerebella processed for immunocytochemical labeling against CAD-28k. The remaining IC animals were housed under standard conditions for re-socialization between postnatal days 32 and 62. The results indicate that Purkinje cells of rats reared under early isolation show a marked loss of CAD-28k immunoreactivity, and that this deficiency is recovered by later social interaction, although the thickness of the molecular layer remains reduced.
Pascual
R
R
Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Spain. rpascual@bbs-ce.uab.es
Verdú
E
E
Valero
A
A
Navarro
X
X
D000818:Animals; D064026:Calbindins; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012931:Social Environment; D012934:Social Isolation
D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G