title: Synthesis and phorbol ester-binding studies of the individual cysteine-rich motifs of protein kinase D.
abstract: To investigate the phorbol ester-binding properties of the individual cysteine-rich motifs of protein kinase D (PKD), the 52-mer peptides containing each cysteine-rich motif of PKD (PKD-C1A, PKD-C1B) have been synthesized. The [3H]phorbol-12,13-dibutyrate (PDBu) binding to PKD-C1A was affected drastically by incubation temperature while that to PKD-C1B was not. Scatchard analysis of [3H]PDBu binding to both PKD C1 peptides gave dissociation constants of 2.5 +/- 0.4 and 2.7 +/- 0.8 nM for PKD-C1A and PKD-C1B, respectively, indicating that the two cysteine-rich motifs of PKD are functionally equivalent like those of PKCgamma.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Irie
authors.forename: A
authors.initials: A
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Ohigashi
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
authors.forename: P A
authors.initials: PA
authors.lastname: Wender
authors.forename: H
authors.initials: H
authors.lastname: Konishi
authors.forename: U
authors.initials: U
authors.lastname: Kikkawa
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry*; D008969:Molecular Sequence Data; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015240:Phorbol 12,13-Dibutyrate; C089212:protein kinase D; D011493:Protein Kinase C; D003545:Cysteine