title: Protective capacity of the Pasteurella haemolytica transferrin-binding proteins TbpA and TbpB in cattle.
abstract: The transferrin-binding proteins TbpA and TbpB from Pasteurella haemolytica biotype A serotype 1 were tested for their ability to confer protection against experimental P. haemolytica infection when administered to calves in vaccine formulations containing one or both antigens. Vaccine groups included TbpB (single immunization), TbpB (two immunizations), TbpA, TbpA+TbpB and a placebo. All animals that received TbpB had measurable antibody titres against the antigen at the time of challenge, while those that received TbpA did not show an antibody response. The TbpA+TbpB group showed the best protection against experimental challenge. Protection correlated with anti-TbpB antibody levels. The enhanced protection in the TbpA+TbpB group suggests TbpA contributed to protection through the induction of a non-antibody-mediated immune response. Sera from the TbpB-immunized animals was cross-reactive with TbpBs from other P. haemolytica serotypes.
authors.affiliation: Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, S7N 5E3, Canada.
authors.forename: A A
authors.initials: AA
authors.lastname: Potter
authors.forename: A B
authors.initials: AB
authors.lastname: Schryvers
authors.forename: J A
authors.initials: JA
authors.lastname: Ogunnariwo
authors.forename: W A
authors.initials: WA
authors.lastname: Hutchins
authors.forename: R Y
authors.initials: RY
authors.lastname: Lo
authors.forename: T
authors.initials: T
authors.lastname: Watts
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D002352:Carrier Proteins / Q000276:immunology / Q000627:therapeutic use*; D002417:Cattle; D003429:Cross Reactions; D033862:Iron-Binding Proteins; D016978:Mannheimia haemolytica / Q000276:immunology*; D012766:Pasteurellosis, Pneumonic / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D033863:Transferrin-Binding Proteins; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins