Capillary electrophoretic analysis of recombinant human apolipoprotein E. Calibration mode of a protein reference material.
Actual lack of standardization of serum apolipoprotein (apo) E measurements prevents intensive studies on the significance of apoE concentration in clinical chemistry. For this purpose the use of standards calibrated against a common reference material is essential in order to obtain reliable results. The assignment of a certified value to this reference material involves the use of an accurate and non immunological technique. We demonstrate here that capillary electrophoresis in sodium dodecyl sulfate containing gel (SDS-CGE) can be a method of choice. ApoE quantification was performed according to the peak area and a standard solution of purified apoAI. We obtained very close results to that obtained by HPLC of phenylalanine apoE content measurement i.e., 81.6 mg/l versus 84.6 mg/l and concluded that the SDS-CGE analysis is an accurate and reliable method for the certification of an apoE reference material to be used in serum apoE concentration measurements. The SDS-CGE combines advantages of SDS-polyacrylamide gel electrophoresis (high resolving power, rapidity and tolerance of complex sample) with that of quantitative HPLC analysis (accuracy, precision, linearity and speed).
Schlenck
A
A
INSERM U525, Centre de Médecine Préventive, Vandoeuvre lès Nancy, France.
Schiele
F
F
Barbier
A
A
Shuvaev
V V
VV
Visvikis
S
S
Siest
G
G
D053318:Apolipoprotein E3; D001057:Apolipoproteins E / Q000032:analysis* / Q000235:genetics; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D011994:Recombinant Proteins / Q000032:analysis; D012015:Reference Standards; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D053318:Apolipoprotein E3; D001057:Apolipoproteins E; D011994:Recombinant Proteins; D012967:Sodium Dodecyl Sulfate