title: Listeria monocytogenes infection of Caco-2 human epithelial cells induces activation of transcription factor NF-kappa B/Rel-like DNA binding activities.
abstract: The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3, which were identified as containing either, RelA and p50, RelB and p50, or p50, respectively. NF-kappa B activation in L. monocytogenes-infected Caco-2 cells was distinct from NF-kappa B activation in infected P388D1 macrophages concerning the NF-kappa B complexes formed and the kinetics of the induction.
authors.affiliation: Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Germany. kuhn@biozentrum.uni-wuerzburg.de
authors.forename: N
authors.initials: N
authors.lastname: Hauf
authors.forename: W
authors.initials: W
authors.lastname: Goebel
authors.forename: F
authors.initials: F
authors.lastname: Fiedler
authors.forename: M
authors.initials: M
authors.lastname: Kuhn
mesh_terms: D018938:Caco-2 Cells / Q000187:drug effects / Q000382:microbiology; D015169:Colony Count, Microbial; D006801:Humans; D008025:Ligases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D013682:Teichoic Acids / Q000494:pharmacology; D051841:Transcription Factor RelB; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D011518:Proto-Oncogene Proteins; C497593:RELB protein, human; D013682:Teichoic Acids; D014157:Transcription Factors; D051841:Transcription Factor RelB; C009900:lipoteichoic acid; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases