Towards molecular farming in the future: transient protein expression in plants.
Molecular farming in plants can be achieved by stable or transient expression of a recombinant protein. Transient expression of recombinant proteins in plants can rapidly provide large amounts of the proteins for detailed characterization. It is fast, flexible and can be carried out at field scale using viral vectors, but it lacks the increases in production volume that can be achieved easily with stable transgenic crops. This review article focuses on discussing the applications of transient expression using viral vectors, biolistic methods or agroinfiltration.
Fischer
R
R
Fraunhofer Department for Molecular Biotechnology, IUCT, Grafschaft, Auf dem Aberg 1, D-57392 Schmallenberg, Germany.
Vaquero-Martin
C
C
Sack
M
M
Drossard
J
J
Emans
N
N
Commandeur
U
U
D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D005544:Forecasting; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000821:virology; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods* / Q000639:trends; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D000906:Antibodies; D011994:Recombinant Proteins