In vitro selective RNA synthesis with L-A virus nanoparticles.
New in vitro RNA synthesis has been performed with an L-A virus nanoparticles, in which the gene and polymerase are integrated. The specific recognition sequence (packaging site) of L-A virus was inserted within a gene of interest. Based on the intrinsic replication cycle, the exogenous RNA with the packaging site was encapsulated by an empty L-A virus nanoparticle. The packaging site worked as a recognition site even for exogenous RNAs. The recognized RNA was replicated to dsRNA, and was then transcribed by empty L-A virus nanoparticles. These results indicate that empty L-A virus nanoparticles recognize an exogenous RNA with the packaging site and synthesize RNA in vitro.
Ebihara
T
T
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
Yanagida
Y
Y
Kobatake
E
E
Aizawa
M
M
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005814:Genes, Viral; D010957:Plasmids / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics / Q000502:physiology*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000821:virology; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases