The site of binding of linker histone to the nucleosome does not depend upon the amino termini of core histones.
Using nucleosomes reconstituted on a defined sequence of DNA, we have investigated the question as to whether the N-terminal tails of core histones play a role in determining the site of binding of a linker histone. Reconstitutes used histone cores of three types: intact, lacking the N-terminal H3 tails, or lacking all tails. In each case the same, single defined position for the histone core was observed, using high-resolution mapping. The affinity for binding of linker histone H1(o) was highest for the intact cores, lowest for the tailless cores. However, the location of the linker histone, as judged by micrococcal nuclease protection, was exactly the same in each case, an asymmetric site of about 17 bp to one side of the core particle DNA.
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Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-7305, USA.
Zlatanova
J
J
Leuba
S H
SH
van Holde
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D001665:Binding Sites; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism
D006657:Histones; D009707:Nucleosomes