Characterization of a group of anaerobically induced, fnr-dependent genes of Salmonella typhimurium.
We have previously reported the isolation of a group of anaerobically regulated, fnr-dependent lac fusions in Salmonella typhimurium and have grouped these oxd genes into classes based on map position. In order to identify these genes, we have replaced the original Mud-lac fusion in a member of each oxd class with the much smaller Mud-cam element, cloned the fusion, and determined DNA sequence sufficient to define the oxd gene. Several of the fusions correspond to previously known genes from S. typhimurium or Escherichia coli: oxd-4 = cbiA and oxd-11 = cbiK, oxd-5 = hybB, oxd-7 = dcuB, oxd-8 = moaB, oxd-12 = dmsA, and oxd-14 = napB (aeg-46. 5). Two other fusions correspond to previously unknown loci: oxd-2 encodes an acetate/propionate kinase, and oxd-6 encodes a putative ABC transporter present in S. typhimurium but not in E. coli.
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Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Miller
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D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000084:Acetate Kinase / Q000235:genetics; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins; D029382:Dicarboxylic Acid Transporters; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012486:Salmonella typhimurium / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029382:Dicarboxylic Acid Transporters; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C086256:dcuB protein, E coli; D017851:Phosphotransferases (Carboxyl Group Acceptor); C111066:TdcD protein, E coli; D000084:Acetate Kinase