The influence of ethanol and magnesium on aminoacyl-tRNA synthetases activity in rat's liver.
Ethanol exerts various influences on living organisms. Experiments were conducted in order to determine the influence of ethanol and magnesium on the aminoacyl-tRNA synthetases (aaRS) activity in rat's liver. The experiment was conducted on white Wistar rats of both sexes. The animals were divided into groups of 10 rats each. They received ethanol in their drinking water for 6 weeks. The first group received 5 per cent ethanol, the second received 20 per cent ethanol, the third received 20 per cent ethanol and 200 ppm magnesium, the fourth was the control and received water. After the duration of the experiments the animals were sacrificed using ketamine and the livers were harvested for further testing. Aminoacyl-tRNA synthetases were obtained by salting out with ammonium sulphate. The aminoacyl-tRNA synthetases activity were tested in vitro by using marked amino acids. Ethanol influences aminoacyl-tRNA synthetases activity. The influence of ethanol on the activity of aaRS depended on the size of the dose of ethanol taken by examined animals. At the same time it was revealed that magnesium supplementation limits the negative effect of high ethanol doses.
Pasternak
K
K
Department of Chemistry, Medical University of Lublin, Poland.
D000284:Administration, Oral; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002492:Central Nervous System Depressants / Q000008:administration & dosage*; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D000431:Ethanol; D000604:Amino Acyl-tRNA Synthetases; D008274:Magnesium