A negative regulatory element in a prespore-specific promoter of dictyostelium discoideum(1).
We previously isolated several 'promoter-trap' transformants in which insertion of a promoterless beta-galactosidase gene into the genome caused expression of beta-galactosidase in specific cell types. The upstream flanking region was rescued from one transformant specifically expressing beta-galactosidase in prespore cells. We sequenced the promoter of the gene that is fused in-frame with lacZ and characterised a negative element that inhibits expression in pstO cells (a subtype of prestalk cells). Gel-retardation assays show that a developmentally regulated factor(s) recognises and binds to this element.
Hsu
Y
Y
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK.
Chang
W
W
Newell
P C
PC
Gross
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D004023:Dictyostelium / Q000235:genetics* / Q000502:physiology; D015723:Gene Library; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013170:Spores / Q000235:genetics
D018076:DNA, Complementary