[Studies on iodinated compounds. X. Isolation and purification of iodoglycyltyrosines].
The methods for the isolation and purification of iodoglycyltyrosines [glycyl-3-iodotyrosine (Gly-MIT) and glycyl-3,5-diiodotyrosine (Gly-DIT)] from a reaction mixture were examined by the use of high-performance liquid chromatography (HPLC). Glycyltyrosine (Gly-Tyr) was iodinated with iodine monochloride (ICl) (the molar ratio of Gly-Tyr to ICl was 1:1.5) in 0.1 M NaOH. The synthesized Gly-MIT and Gly-DIT were separated on a mu Bondapak C18 column employing stepwise gradient systems of a 0.1% trifluoroacetic acid/acetonitrile mixture and a water/acetonitrile mixture. Chemically pure Gly-MIT and Gly-DIT were obtained in 30.2% and 28.2% yields, respectively.
Miyashita
M
M
Department of Clinical Chemistry, Hoshi University, Tokyo, Japan.
Matsunaga
R
R
Seyama
Y
Y
Yamashita
S
S
D055598:Chemical Phenomena; D002621:Chemistry; D002712:Chlorides; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004151:Dipeptides; D007454:Iodides; D007470:Monoiodotyrosine / Q000302:isolation & purification*
D002712:Chlorides; D004151:Dipeptides; D007454:Iodides; C009813:iodine monochloride; C022013:glycyltyrosine; D007470:Monoiodotyrosine