Molecular cloning and sequence analysis of Zygosaccharomyces rouxii ADE2 gene encoding a phosphoribosyl-aminoimidazole carboxylase.
The nucleotide sequence of a 2.8 kb fragment containing the ADE2 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The gene was cloned from a Z. rouxii genomic DNA library by complementation of the Saccharomyces cerevisae ade2 mutant strain. The sequenced DNA fragment contains a 1710 bp open reading frame predicting a protein of 570 amino acids. The deduced amino acid sequence shares a high degree of homology with Ade2p homologues in five other yeast species.
Sychrova
H
H
Department of Membrane Transport, Institute of Physiology CzAcadSci, 142 20 Prague 4, Czech Republic. sychrova@biomed.cas.cz
Braun
V
V
Souciet
J L
JL
D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D020068:Zygosaccharomyces / Q000201:enzymology* / Q000235:genetics
D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase