Increased incidence and analysis of emperipolesis in megakaryocytes of the mouse mutant gunmetal.
Mutant gunmetal (gm/gm) mice exhibit prolonged bleeding, platelet granule defects, abnormal megakaryocyte demarcation membranes, and thrombocytopenia. The number of megakaryocytes in gm/gm mice is increased substantially. Also, the percentage of gm/gm megakaryocytes exhibiting emperipolesis is increased. However, the number of emperipolesed cells per megakaryocyte is not. EC are of several hematopoietic lineages, with a slight skew to granulocytes, and include mature, primitive, and degenerating cells. No significant differences in the types of emperipolesed cells were observed between mutant mice and their normal gm/+ or +/+ counterparts. The increased incidence of emperipolesis in gm/gm megakaryocytes is controlled by the megakaryocyte genotype, not systemic factors. A significant practical finding of these studies was the demonstration that increased emperipolesis results in a significant "right shift" in megakaryocyte ploidy determined by flow cytometry.
McGarry
M P
MP
Department of Laboratory Animal Resources, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Reddington
M
M
Jackson
C W
CW
Zhen
L
L
Novak
E K
EK
Swank
R T
RT
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002452:Cell Count; D005260:Female; D005269:Femur; D005434:Flow Cytometry; D008297:Male; D008533:Megakaryocytes / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation; D010587:Phagocytosis; D011003:Ploidies; D013154:Spleen / Q000166:cytology / Q000502:physiology