Cloning, sequence analysis, and expression of ansB from Pseudomonas fluorescens, encoding periplasmic glutaminase/asparaginase.
A gene (ansB) encoding a class II glutaminase/asparaginase has been cloned from Pseudomonas fluorescens and characterized by DNA sequencing, promoter analysis and heterologous expression in Escherichia coli. We show that ansB is monocistronic and depends on the alternate sigma factor sigma 54 for expression. A second open reading frame located downstream of ansB is highly homologous to a number of bacterial genes that encode secreted endonucleases of unknown function.
Hüser
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A
Philipps-Universität, Institut für Physiologische Chemie, Marburg, Germany.
Klöppner
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U
Röhm
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KH
D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D019897:Periplasm / Q000201:enzymology*; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics; D051877:RNA Polymerase Sigma 54; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000378:metabolism
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012808:Sigma Factor; C497751:rpoN protein, E coli; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54; D004720:Endonucleases; D000581:Amidohydrolases; C020654:glutamin-(asparagin-)ase