Detection and characterization of cerein 7, a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity.
A bacteriocin-producing strain of Bacillus cereus was identified and isolated from a soil sample. The bacteriocin could be purified by a two-step procedure: ammonium sulfate precipitation of culture supernatants followed by a butanol extraction step, the antibiotic was recovered from the organic phase. The peptidic nature of the bacteriocin was proven by its sensitivity to proteolytic enzymes; its molecular mass, determined by mass spectrometry, was 3940 Da; and its amino-terminal sequence (GWGDVL) is unique in the databases. The compound was active against most Gram-positive but not Gram-negative bacteria. This is to our knowledge the first bacteriocin with these characteristics reported to be produced by B. cereus.
Oscáriz
J C
JC
Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona, Spain.
Lasa
I
I
Pisabarro
A G
AG
D001409:Bacillus cereus / Q000254:growth & development / Q000378:metabolism*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests
D001430:Bacteriocins