UVB and gamma-radiation induce the expression of mRNAs encoding the ribosomal subunit L13A in rat keratinocytes.
Ultraviolet B radiation produces an array of cellular perturbations in the skin. We isolated a keratinocyte cDNA encoding the rat 60S ribosomal subunit protein L13a following differential cDNA library screening with UVB-enriched probes. In contrast to the reported structure of liver L13a, the keratinocyte L13a cDNA contains a longer 3'-untranslated region. Northern blot analysis detected two L13a mRNA transcripts, approximately 800 bp and approximately 1.2 kb, in keratinocytes and a variety of rat tissues. Both L13a mRNA transcripts were induced by UVB irradiation, forskolin and gamma-irradiation. In contrast, no induction of L13a mRNA transcript levels was observed following exposure of keratinocytes to 12-O-tetradecanoylphorbol-13-acetate, serum and the DNA damage-inducing agents methyl methanesulfonate or 4-nitroquinoline-N-oxide. These observations suggest that increased expression of ribosomal subunit genes may be a molecular component of the keratinocyte response to UVB in particular and not part of a nonspecific response to DNA damage.
Shahmolky
N
N
Department of Clinical Biochemistry, Women's College Hospital, University of Toronto, Canada.
Lefebvre
D L
DL
Poon
R
R
Bai
Y
Y
Sharma
M
M
Rosen
C F
CF
D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects*; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D014466:Ultraviolet Rays
D018076:DNA, Complementary; D012333:RNA, Messenger; C484067:RPL38 protein, rat; D012269:Ribosomal Proteins