The outer membrane of gram-negative bacteria inhibits antibacterial activity of brochocin-C.
Brochocin-C is a two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754 that has a broad activity spectrum comparable to that of nisin. Brochocin-C has an inhibitory effect on EDTA-treated gram-negative bacteria, Salmonella enterica serovar Typhimurium lipopolysaccharide mutants, and spheroplasts of Typhimurium strains LT2 and SL3600. Brochocin-C treatment of cells and spheroplasts of strains of LT2 and SL3600 resulted in hydrolysis of ATP. The outer membrane of gram-negative bacteria protects the cytoplasmic membrane from the action of brochocin-C. It appears that brochocin-C is similar to nisin and possibly does not require a membrane receptor for its function; however, the difference in effect of the two bacteriocins on intracellular ATP indicates that they cause different pore sizes in the cytoplasmic membrane.
Gao
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Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
van Belkum
M J
MJ
Stiles
M E
ME
D000255:Adenosine Triphosphate / Q000378:metabolism; D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006868:Hydrolysis; D009561:Nisin / Q000494:pharmacology
D001430:Bacteriocins; C116792:brochocin C; D009561:Nisin; D000255:Adenosine Triphosphate; D004492:Edetic Acid