title: [Virus reproduction in cell cultures synchronized by macromolecular platinum complexes].
abstract: A new method of application of platinum macromolecular complex poly{hexacis[chloroamine aquaplatinum (II)]}-mu-deoxyribonucleate (MCP) as a cell cycle synchronization inductor has been developed. The process of some RNA-genome viruses reproduction in cell cultures synchronized by MCP was studied. It was shown that the amount of virus protection in such cultures under special conditions can be increased 100-350 times without increasing the cell substrate amount. The obtained results may be used in biotechnology to increase production of virus antigens and vaccines.
authors.affiliation: Kyiv Medical Academy of Postgraduate Education, Ministry of Public Health of Ukraine.
authors.forename: V M
authors.initials: VM
authors.lastname: Hyrin
authors.forename: I V
authors.initials: IV
authors.lastname: Dziublyk
authors.forename: O P
authors.initials: OP
authors.lastname: Trokhymenko
authors.forename: V K
authors.initials: VK
authors.lastname: Koval'chuk
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D012328:RNA Viruses / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D046911:Macromolecular Substances; D009944:Organoplatinum Compounds