Development and evaluation of a direct immunofluorimetric assay for urinary growth hormone.
We describe a two-site immunofluorimetric assay for urinary growth hormone that is resistant to interference from a wide range of urinary constituents and therefore eliminates the need for sample pre-treatment. A microtitre plate format is used with specific orientation of capture antibody on a polystyrene surface carrying a hydrazide group. Europium-labelled F(ab)2 is the fluorophore and time-resolved fluorimetry with co-fluorescence enhancement the signal detection system. Inter-assay precision was 11.3% at 5.2 ng/L and 10.3% at 44.3 ng/L, minimum detection limit (22% coefficient of variation, CV) was < 1.0 ng/L, working range (< 10% CV) was 0-100 ng/L and quantitative recovery and good parallelism were demonstrated. This convenient and sensitive assay is suitable for the routine measurement of human growth hormone (hGH) in urine.
McConway
M G
MG
Scottish Antibody Production Unit, Law Hospital, Carluke, UK.
Smith
K A
KA
Beastall
G H
GH
D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique / Q000592:standards*; D019382:Human Growth Hormone / Q000276:immunology / Q000652:urine*; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D019382:Human Growth Hormone