title: Cross-bred cattle protected against Hyalomma anatolicum anatolicum by larval antigens purified by immunoaffinity chromatography.
abstract: Antigens from larvae of Hyalomma anatolicum anatolicum were extracted and purified by immunoaffinity chromatography using immunoglobulin ligands from cross-bred animals immunized with soluble larval antigen. Affinity-purified antigen (Aff-TLE) and a total larval extract (TLE) were used to immunize cross-bred (Bos indicus x Bos taurus) cattle. The group immunized with Aff-TLE rejected 71.6% of larvae and 77.3% of nymphs. However, the rejection percentages were lower in the TLE-immunized group. No significant changes in the feeding period, moulting percentages or moulting period of engorged larvae and nymphs were recorded. There was, however, a significant decrease in the number of resultant nymphs p < 0.01) and adults (p < 0.01) in the ticks fed on the Aff-TLE-immunized group. The Aff-TLE antigen was 93.3% purified. SDS-PAGE analysis identified a 39 kDa protein, reported for the first time, as the antigen responsible for the induction of resistance in the host.
authors.affiliation: Entomology Laboratory, Indian Veterinary Research Institute, Izatnagar, Pradesh, India.
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: M H
authors.initials: MH
authors.lastname: Khan
authors.forename: N
authors.initials: N
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D002846:Chromatography, Affinity / Q000662:veterinary; D002848:Chromatography, DEAE-Cellulose / Q000662:veterinary; D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007114:Immunization / Q000662:veterinary; D007814:Larva / Q000276:immunology; D008297:Male; D011897:Random Allocation; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000276:immunology*
chemical_list: D000906:Antibodies; D000941:Antigens