Dermal absorption and distribution of topically dosed jet fuels jet-A, JP-8, and JP-8(100).
Dermal exposure to jet fuels has received increased attention with the recent release of newer fuels with novel performance additives. The purpose of these studies was to assess the percutaneous absorption and cutaneous disposition of topically applied (25 microl/5 cm(2)) neat Jet-A, JP-8, and JP-8(100) jet fuels by monitoring the absorptive flux of the marker components 14C naphthalene and (3)H dodecane simultaneously applied nonoccluded to isolated perfused porcine skin flaps (IPPSF) (n = 4). Absorption of 14C hexadecane was estimated from JP-8 fuel. Absorption and disposition of naphthalene and dodecane were also monitored using a nonvolatile JP-8 fraction reflecting exposure to residual fuel that might occur 24 h after a jet fuel spill. In all studies, perfusate, stratum corneum, and skin concentrations were measured over 5 h. Naphthalene absorption had a clear peak absorptive flux at less than 1 h, while dodecane and hexadecane had prolonged, albeit significantly lower, absorption flux profiles. Within JP-8, the rank order of absorption for all marker components was (mean +/- SEM % dose) naphthalene (1.17 +/- 0.07) > dodecane (0.63 +/- 0.04) > hexadecane (0.18 +/- 0.08). In contrast, deposition within dosed skin showed the reverse pattern. Naphthalene absorption into perfusate was similar across all fuel types, however total penetration into and through skin was highest with JP-8(100). Dodecane absorption and total penetration was greatest from JP-8. Absorption of both markers from aged JP-8 was lower than other fuels, yet the ratio of skin deposition to absorption was greatest for this treatment group. In most exposure scenarios, absorption into perfusate did not directly correlate to residual skin concentrations. These studies demonstrated different absorption profiles for the three marker compounds, differential effects of jet fuel types on naphthalene and dodecane absorption, and uncoupling of perfusate absorption from skin disposition.
Riviere
J E
JE
Center for Cutaneous Toxicology and Residue Pharmacology, College of Veterinary Medicine, Raleigh, North Carolina 27606, USA. Jim_Riviere@NCSU.EDU
Brooks
J D
JD
Monteiro-Riviere
N A
NA
Budsaba
K
K
Smith
C E
CE
D000287:Administration, Topical; D000401:Aircraft; D000473:Alkanes / Q000493:pharmacokinetics*; D000818:Animals; D005648:Fuel Oils; D009281:Naphthalenes / Q000493:pharmacokinetics*; D010477:Perfusion; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013552:Swine
D000473:Alkanes; D005648:Fuel Oils; D009281:Naphthalenes; C007548:n-dodecane; C031721:naphthalene; C007932:n-hexadecane