title: Human glomerular endothelial cells IGFBPs are regulated by IGF-I and TGF-beta1.
abstract: The release of insulin-like growth factor binding proteins (IGFBPs) and their regulation in human glomerular endothelial cells (GENC) was characterised. GENC produce IGFBP-4, IGFBP-2 and IGFBP-3 and express mRNA for IGFBP-2 to IGFBP-5. Due to the fact that IGF-I and TGF-beta1 modulate glomerular hypertrophy, their action on IGFBP release and GENC growth was studied. IGF-I increased IGFBP-3, IGFBP-2 and decreased IGFBP-4, while TGF-beta1 decreased IGFBP-3 and apparently increased IGFBP-4. All of the IGFBPs, except the TGF-beta1-regulated IGFBP-4, were modulated at mRNA level. IGF-I stimulated GENC proliferation, while TGF-beta1 inhibited their growth. It was demonstrated that an IGFBP-3 antibody reduced GENC proliferation. However, rhIGFBP-3 alone had no effect on GENC, but after 48 h pre-incubation the IGF-I stimulated GENC growth was increased, suggesting that IGFBP-3 could modulate the IGF-I induced GENC proliferation. It was concluded that the stimulatory IGFBP-3 and the inhibitory IGFBP-4 could regulate GENC growth, although the IGFBP-3 seems to have a predominant effect in this control.
authors.affiliation: Department of Clinical Pathophysiology, University of Florence, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Giannini
authors.forename: B
authors.initials: B
authors.lastname: Cresci
authors.forename: L
authors.initials: L
authors.lastname: Pala
authors.forename: A
authors.initials: A
authors.lastname: Ciucci
authors.forename: C
authors.initials: C
authors.lastname: Manuelli
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita-Yamaguchi
authors.forename: P
authors.initials: P
authors.lastname: Cappugi
authors.forename: C M
authors.initials: CM
authors.lastname: Rotella
mesh_terms: D002455:Cell Division / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry; D004727:Endothelium / Q000737:chemistry* / Q000166:cytology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000187:drug effects* / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D007678:Kidney Glomerulus / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D017077:Culture Media, Conditioned; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I