Novel canine tumour suppressor gene p53 mutations in cases of skin and mammary neoplasms.
Exons 4 to 8 of the tumour suppressor gene p53 were analysed in 25 skin and 25 mammary tumours of 50 dogs. A 1 bp deletion (ACC-->AC) was detected in codon 89 in exon 4 in a squamous cell carcinoma. A missense mutation CGC-->CAC (arginine-->histidine) was present in codon 162 in exon 5 in a mammary adenocarcinoma. Moreover, a silent mutation occurred in codon 103 (serine) of exon 4 in a mammary adenoma. The somatic nature of the three mutations was demonstrated.
Mayr
B
B
Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
Reifinger
M
M
Alton
K
K
D000230:Adenocarcinoma / Q000235:genetics / Q000662:veterinary; D000236:Adenoma / Q000235:genetics / Q000662:veterinary; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000662:veterinary; D003062:Codon / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000737:chemistry; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D016368:Frameshift Mutation; D016158:Genes, p53 / Q000235:genetics*; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D012878:Skin Neoplasms / Q000235:genetics / Q000662:veterinary*
D003062:Codon; D017931:DNA Primers; D004273:DNA, Neoplasm