Migratory mechanisms of chick primordial germ cells toward gonadal anlage.
After appearing at the germinal crescent region, chick primordial germ cells (PGCs) migrate toward the presumptive gonads (pG) till stage 19 (Hamburger and Hamilton, 1951). This study seeks to elucidate the roles of passive and active factors in the PGC-migration, physical trapping of circulating PGCs by the capillary network and PGC attraction by chemotactic factor from presumptive gonads. Firstly, latex beads/pollens (the same size or larger than PGCs) were injected into the embryonic bloodstream at stage 13-19 (when PGCs are in the migrating and settlement phase to the presumptive gonad) in ovo in order to determine whether the PGCs passively reach pG. Most of such particles accumulated in the head region (60%), whereas the remainder did the same in the gonadal region (23% at the peak) at stage 16 when both the head and gonadal regions are rich in capillary plexus. After 3 days, most particles in the gonadal region were located at the angles of dorsal mesentery near the developing gonads where many extra-gonadal PGCs had been located, and a few particles were detected close to the gonad. These results suggest that one of the mechanisms of PGC-migration to the developing gonads is an autonomous trapping of PGCs by the capillary network quite close to the germinal epithelium (GE) and passive translocation by morphogenetic movement. Secondly, the attraction for PGCs by the gonadal anlage proper was examined in ovo using chick and quail embryos. Grafts of quail gonadal anlage containing gonadal epithelium and neighbouring mesenchymal tissue were excised from the quail embryo at stages 12 to 16 (staging by Zacchei, 1961). With the aims of eliminating the influence of surrounding tissue, the quail graft was ectopically transplanted into the posterior to the optic vesicle of 8 to 17 somite chick embryo from the point of a posterior region to the auditory vesicle by a fine tungsten needle under the illumination by the method of Hara (1971). Then the region posterior to the level of presumptive vitelline arteries was surgically excised in ovo. After a 48 hrs.-incubation, the host PGCs which lost their own gonadal anlage as a target organ accumulated in the transplanted quail gonadal anlage originating from the embryo at PGC-migrating periods. This result strongly suggested the presence of some attractive factor that may be emitted from the gonadal anlage proper. Furthermore, it was demonstrated that the PGCs in vitro showed no contact inhibition in relation to other PGCs or fibroblasts in their moving pathway.
Kuwana
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Pathology Section, National Institute for Minamata Disease, Minamata City, Kumamoto, Japan. kuwana@fsinet.or.jp
Rogulska
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D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D002642:Chick Embryo; D004848:Epithelium / Q000196:embryology; D005854:Germ Cells / Q000502:physiology*; D006066:Gonads / Q000166:cytology / Q000196:embryology; D014741:Video Recording