Reconstruction of an active integron recombination site after integration of a gene cassette at a secondary site.
As the site of insertion of the aadB gene cassette on pRAY, from a clinical isolate of Acinetobacter, is almost identical to the preferred site on integrons, the composite 59-base element (59-BE) associated with this cassette is potentially recombinationally active. By using a conduction assay to quantitate site activity, the 59-BE was recognized by integrase with high frequency, indicating that the composite site is recombinationally active.
Segal
H
H
Department of Medical Microbiology, University of Cape Town, South Africa.
Francia
M V
MV
Lobo
J M
JM
Elisha
G
G
D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001665:Binding Sites; D019426:Integrases / Q000378:metabolism; D011995:Recombination, Genetic; D015183:Restriction Mapping
C107954:AadB protein, Acinetobacter sp. SUN; D001426:Bacterial Proteins; D019426:Integrases