Human apolipoprotein A-I kinetics within triglyceride-rich lipoproteins and high density lipoproteins.
Stable isotope methodology was used to determine the kinetic behavior of apolipoprotein (apo) A-I within the triglyceride-rich lipoprotein (TRL) fraction and to compare TRL apoA-I kinetics with that of apoA-I in high density lipoprotein (HDL) and TRL apoB-48. Eight subjects (5 males and 3 females) over the age of 40 were placed on a baseline average American diet and after 6 weeks received a primed-constant infusion of [5,5,5-(2)H(3)]-l-leucine for 15 h while consuming small hourly meals of identical composition. HDL and TRL apoA-I and TRL apoB-48 tracer/tracee enrichment curves were obtained by gas chromatography;-mass spectrometry. Data were fitted to a compartmental model to determine the fractional secretion rates of apoA-I and apoB-48 within each lipoprotein fraction. Mean plasma apoA-I levels in TRL and HDL fractions were 0. 204 +/- 0.057 and 134 +/- 15 mg/dl, respectively. The mean fractional catabolic rate (FCR) of TRL apoA-I was 0.250 +/- 0.069 and HDL apoA-I was 0.239 +/- 0.054 pools/day, with mean estimated residence times (RT) of 4.27 and 4.37 days, respectively. The mean TRL apoB-48 FCR was 5.2 +/- 2.0 pools/day and the estimated mean RT was 5.1 +/- 1.8 h. Our results indicate that apoA-I is catabolized at a slower rate than apoB-48 within TRL, and that apoA-I within TRL and HDL fractions are catabolized at similar rates.
Vélez-Carrasco
W
W
Lipid Metabolism Laboratory, Mass Spectrometry Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA.
Lichtenstein
A H
AH
Barrett
P H
PH
Sun
Z
Z
Dolnikowski
G G
GG
Welty
F K
FK
Schaefer
E J
EJ
D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood*; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000097:blood; D005260:Female; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood*
D016632:Apolipoprotein A-I; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D014280:Triglycerides