Identification of cohesin association sites at centromeres and along chromosome arms.
A multisubunit cohesin complex holds sister chromatids together after DNA replication. Using chromatin immunoprecipitation, we detected cohesin association with centromeres and with discrete sites along chromosome arms from S phase until metaphase in S. cerevisiae. Short DNA sequences (130-280 bp) are sufficient to confer cohesin association. Cohesin association with a centromere depends on Mif2p, the centromere binding factor CBF3, and a centromere-specific histone variant, Cse4p. Because only active centromeres confer cohesin association with centromeric DNA, we suggest that cohesin is recruited by the same chromatin structure that confers the attachment of microtubules. Propagation of this structure might be partly epigenetic. Finally, cohesion associated with "minimal" centromeres is insufficient to resist the splitting force exerted by microtubules and appears to be reinforced by cohesion provided by their flanking DNA sequences.
Tanaka
T
T
Research Institute of Molecular Pathology, Vienna, Austria.
Cosma
M P
MP
Wirth
K
K
Nasmyth
K
K
D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000302:isolation & purification; D002503:Centromere / Q000737:chemistry* / Q000648:ultrastructure; D011508:Chondroitin Sulfate Proteoglycans; D002842:Chromatids / Q000737:chemistry* / Q000648:ultrastructure; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification; D015825:Chromosomes, Fungal / Q000737:chemistry* / Q000648:ultrastructure; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D018386:Kinetochores / Q000737:chemistry / Q000378:metabolism; D009687:Nuclear Proteins / Q000302:isolation & purification*; D010750:Phosphoproteins; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1
D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006657:Histones; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1; C092348:CEP3 protein, S cerevisiae; C092746:CSE4 protein, S cerevisiae; C490529:MCD1 protein, S cerevisiae; C083768:MIF2 protein, S cerevisiae; C120372:SMC3 protein, S cerevisiae