Interstrain variation of the polysaccharide B biosynthesis locus of Bacteroides fragilis: characterization of the region from strain 638R.
The sequence and analysis of the capsular polysaccharide biosynthesis locus, PS B2, of Bacteroides fragilis 638R are described, and the sequence is compared with that of the PS B1 biosynthesis locus of B. fragilis NCTC 9343. Two genes of the region, wcgD and wcgC, are shown by complementation to encode a UDP-N-acetylglucosamine 2-epimerase and a UDP-N-acetylmannosamine dehydrogenase, respectively.
Comstock
L E
LE
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. lcomstock@channing.harvard.edu
Coyne
M J
MJ
Tzianabos
A O
AO
Kasper
D L
DL
D016667:Bacterial Capsules / Q000096:biosynthesis*; D001441:Bacteroides fragilis / Q000201:enzymology / Q000235:genetics*; D002237:Carbohydrate Dehydrogenases / Q000235:genetics*; D002238:Carbohydrate Epimerases / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data
D029968:Escherichia coli Proteins; D002237:Carbohydrate Dehydrogenases; C023228:UDP-N-acetylmannosamine dehydrogenase; D002238:Carbohydrate Epimerases; C100033:UDP acetylglucosamine-2-epimerase; C083982:wecB protein, E coli