Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV.
Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV); infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.
Lorenzen
E
E
Danish Veterinary Laboratory, EU Reference Laboratory for Fish Diseases, Arhus, Denmark. el@svs.dk
Carstensen
B
B
Olesen
N J
NJ
D000818:Animals; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D002347:Carps; D002460:Cell Line / Q000821:virology*; D003530:Cyprinidae; D005393:Fish Diseases / Q000175:diagnosis / Q000821:virology; D018061:Infectious pancreatic necrosis virus / Q000254:growth & development / Q000302:isolation & purification*; D007753:Laboratories / Q000592:standards; D017686:Oncorhynchus mykiss; D010473:Perciformes; D015203:Reproducibility of Results; D012209:Rhabdoviridae / Q000254:growth & development / Q000302:isolation & purification*; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D012474:Salmon / Q000196:embryology