Assessment of bovine leukemia virus transcripts in vivo.
Reverse transcriptase PCR (RT-PCR) consistently detected bovine leukemia virus transcripts in fresh cells, and competitive RT-PCR enumerated these transcripts. The detection of transcripts in limited numbers of tumor cells indicated that expression occurs in a minority of cells. The data suggest that individual cells contain hundreds of copies of the tax/rex transcript in vivo.
Rovnak
J
J
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA. jr38@cornell.edu
Casey
J W
JW
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic