Pseudomonas aeruginosa phage lysate as an immunobiological agent. 1. Selection of Pseudomonas aeruginosa clinical strains for phage lysate preparation.
A total of 2087 Pseudomonas aeruginosa isolates collected during the period 1994-1997 were used as starting material. Out of 1704 in-patient isolates, 299 strains were selected for the preparation of phage lysates but only five strains provided stable lysates, i.e., maintained the ability to be repeatedly and completely lysed by the appropriate phage in the course of several years. A set of 193 out-patients (189) and water sources (4) isolates failed to yield strains suitable for phage lysate preparation; 190 strains isolated abroad from patients with cystic fibrosis or respiratory infections included three isolates which, despite having a high degree of mucus production, were suitable for lysate preparation. The antigenic pattern of the phage lysates was ascertained by SDS-polyacrylamide gel electrophoresis.
Sekaninová
G
G
Department of Epidemiology of Infectious Diseases, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Kolárová
M
M
Pillich
J
J
Seménka
J
J
Slavíková
H
H
Kubícková
D
D
Zajícová
V
V
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001434:Bacteriophage Typing; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D017105:Pseudomonas Phages / Q000145:classification / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000821:virology*
D000942:Antigens, Bacterial