Rapid method for the identification of essential genes in Staphylococcus aureus.
A strategy based on a vector host-dependent for autonomous replication, pSA3182, was utilized both for the rapid screening for Staphylococcus aureus genes essential for cell viability and for the introduction of specific polarity-neutral deletions in nonessential genes. The results obtained support the use of pSA3182 for both purposes.
Xia
M
M
Public Health Research Institute, New York, New York 10016, USA.
Lunsford
R D
RD
McDevitt
D
D
Iordanescu
S
S
D001426:Bacterial Proteins / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins; D053487:DEAD-box RNA Helicases; D004268:DNA-Binding Proteins / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D020043:Genes, Essential; D051839:NFI Transcription Factors; D010445:Peptide Elongation Factors / Q000235:genetics; D020365:RNA Helicases / Q000235:genetics; D016601:RNA-Binding Proteins; D013211:Staphylococcus aureus / Q000235:genetics*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D051839:NFI Transcription Factors; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C065908:dbpA protein, E coli; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases