title: Expression of pulmonary mRNA encoding ICAM-1, VCAM-1, and P-selectin following thoracic irradiation in mice.
abstract: OBJECTIVE
  Recent studies have revealed that ionizing radiation induces the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and P-selectin in vitro. The purpose of this study was to investigate the expression of these adhesion molecules in mouse lung following whole thoracic irradiation.
  
  
  METHODS
  C57BL/6J mice were irradiated with a single dose of 12 Gy to the thoraces and sacrificed at 4, 12, 24, and 48 hours and 1, 2, 4, and 8 weeks after irradiation. Expression of total lung mRNA for ICAM-1, VCAM-1, and P-selectin was quantified by the Northern blot method and normalized to beta-actin.
  
  
  RESULTS
  There were increases in mRNA for ICAM-1 of 42% at 4 hours (p < 0.05), 76% at 24 hours (p < 0.01), and 51% at 48 hours (p < 0.05) compared with the controls. These returned to the control level at 1 week. The expression of VCAM-1 mRNA was also increased by 49% (p < 0.01) at 12 hours and was still increased by 25% at 1 week. P-selectin mRNA was transiently increased by 59% at 12 hours.
  
  
  CONCLUSIONS
  These early inductions of mRNA for ICAM-1, VCAM-1, and P-selectin in mouse lung following thoracic irradiation were transient but significant, and are one of the most immediate changes reported in vivo.
authors.affiliation: Department of Radiology, Kobe University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tsujino
authors.forename: A
authors.initials: A
authors.lastname: Kodama
authors.forename: N
authors.initials: N
authors.lastname: Kanaoka
authors.forename: T
authors.initials: T
authors.lastname: Maruta
authors.forename: M
authors.initials: M
authors.lastname: Kono
mesh_terms: D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D004307:Dose-Response Relationship, Radiation; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics*; D008168:Lung / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019007:P-Selectin / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D013909:Thorax / Q000528:radiation effects*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D015415:Biomarkers; D019007:P-Selectin; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1