title: Duration of immunity, efficacy and safety in sheep of a recombinant Taenia ovis vaccine formulated with saponin or selected adjuvants.
abstract: The efficacy and safety of a recombinant Taenia ovis protein was tested in sheep using 13 different adjuvant formulations, including oil adjuvants, aluminium salts, saponin, Iscoms and DEAE-dextran. The oil adjuvants, saponin and DEAE-dextran gave the highest antibody responses and greatest degree of protection against challenge infection with T. ovis eggs. Duration of immunity studies with a saponin based vaccine showed that highly significant protection (>90% reduction of cyst numbers) was achieved when sheep were challenge infected one month after immunisation. Significant protection (79%) was still present when sheep were challenged 6 months after immunisation. The optimum dose for this batch of saponin was 10 mg, which stimulated a peak antibody titre of 38,400, 4 weeks after immunisation and did not cause injection site reactions. Dialysed saponin was shown to retain its adjuvant properties and allowed an increase in dose to 30 mg without site reaction, resulting in a peak antibody titre of 51,200.
authors.affiliation: Ag Research, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. harrisong@agresearch.cri.nz
authors.forename: G B
authors.initials: GB
authors.lastname: Harrison
authors.forename: T R
authors.initials: TR
authors.lastname: Shakes
authors.forename: C M
authors.initials: CM
authors.lastname: Robinson
authors.forename: S B
authors.initials: SB
authors.lastname: Lawrence
authors.forename: D D
authors.initials: DD
authors.lastname: Heath
authors.forename: R P
authors.initials: RP
authors.lastname: Dempster
authors.forename: M W
authors.initials: MW
authors.lastname: Lightowlers
authors.forename: M D
authors.initials: MD
authors.lastname: Rickard
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D003637:DEAE-Dextran; D012503:Saponins; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000517:prevention & control / Q000662:veterinary*; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D012503:Saponins; D014612:Vaccines; D014614:Vaccines, Synthetic; D003637:DEAE-Dextran