Interaction of cyclophilin and cyclosporins monitored by affinity capillary electrophoresis.
The affinity capillary electrophoretic separation of the complex of the enzyme cyclophilin (Cyp) with the immunosuppressive drug cyclosporin A (CsA) from uncomplexed Cyp and CsA in phosphate buffer (pH 8) under non-denaturing conditions by equilibrium-mixture analysis is reported. Using a new approach combining mobility-shift analysis and electrophoretically mediated microanalysis the binding constant of rhCyp18 to CsA and derivatives was estimated.
Kiessig
S
S
Max-Planck Research Division Enyzmology of Protein Folding, Halle/Saale, Germany.
Bang
H
H
Thunecke
F
F
D003524:Cyclosporins / Q000302:isolation & purification* / Q000494:pharmacology; D004347:Drug Interactions; D016903:Drug Monitoring / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000302:isolation & purification / Q000494:pharmacology; D019696:Peptidylprolyl Isomerase / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology
D003524:Cyclosporins; D004791:Enzyme Inhibitors; D007166:Immunosuppressive Agents; D019696:Peptidylprolyl Isomerase