Proviral DNA in the brains of goats infected with caprine arthritis-encephalitis virus.
Archive paraffin wax-embedded sections of brain from goats and kids naturally infected with caprine arthritis-encephalitis virus (CAEV) were examined. Severe leucoencephalitis was present, with infiltration of lymphocytes, plasma cells and macrophages into the white matter, meninges and choroid plexus. On both CAEV-positive and negative (control) tissues, in-situ polymerase chain reactions were used to amplify a DNA sequence specific to the proviral Pol region. In the infected tissues, strong hybridization signals were observed, mainly located in macrophages, microglial cells, astrocytes and oligodendrocytes, and in the ependymal epithelium and choroid plexus. Positive areas were also found in the spinal cord in endothelial cells of small blood vessels. Some neurons showed a positive reaction.
Sanna
E
E
Istituto di Patologia Generale, Anatomia Patologica e Clinica Ostetrico-Chirurgica Veterinaria, Università degli Studi di Sassari, Via Vienna n. 2, Sassari, 07100, Italy.
Sanna
M P
MP
Vitali
C G
CG
Renzoni
G
G
Sanna
L
L
Spano
S
S
Rossi
G
G
Leoni
A
A
D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000302:isolation & purification*; D001921:Brain / Q000098:blood supply / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D004730:Endothelium, Vascular / Q000473:pathology / Q000821:virology; D015511:Goat Diseases / Q000473:pathology / Q000821:virology*; D006041:Goats / Q000821:virology; D016180:Lentivirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017628:Microglia / Q000473:pathology / Q000821:virology; D009474:Neurons / Q000473:pathology / Q000821:virology; D009836:Oligodendroglia / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification; D013116:Spinal Cord / Q000473:pathology / Q000821:virology
D004279:DNA, Viral