Transcriptional analysis of the cyclophilin A gene (cypA) of Streptomyces chrysomallus.
Streptomyces chrysomallus produces a 17.5-kDa cyclophilin (CypA) as a major cytosolic protein. Northern blotting of total RNA from S. chrysomallus showed that cypA is transcribed as a monocistronic 500-nt transcript during the vegetative growth phase only. Consistently, Western blot analysis and enzyme activity determinations revealed a high level of CypA which declined drastically when cultures entered postexponential phase. Primer extension experiments revealed that cypA is transcribed as a leaderless transcript with the startpoint of transcription and translation being the same AUG codon. Analysis of -10 and -35 regions revealed an EohrdB-specific promoter consensus sequence in accordance with the observed transcription during vegetative growth of cultures. The leaderless cypA mRNA sequence between codons 5 to 12 shows complementarity to an internal antidownstream box of 16S rRNA of Streptomyces lividans. This may indicate a possible interaction of the leaderless cypA transcript with the 16S mRNA in translation initiation.
Berger
R
R
Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Technische Universität Berlin, Berlin-Charlottenburg, Germany.
Schauwecker
F
F
Keller
U
U
D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
D012329:RNA, Bacterial; D012333:RNA, Messenger; D019696:Peptidylprolyl Isomerase