Insertional gene fusion technology.
The classical 'end to end' gene fusion technique has widely been used for monitoring gene expression, biological screening and purification of recombinant proteins. Recent progress with the 'insertional' gene fusion approach, on the other hand, has demonstrated that this technique can be utilized for membrane protein topology analysis, display of randomized protein libraries and design of biosensor proteins. In this review, we describe examples of insertional gene fusion and compare the old and new gene fusion techniques.
Doi
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Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Yanagawa
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D019677:Artificial Gene Fusion / Q000379:methods*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000379:methods*; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins