Isolation and purification of a 316 Da preformed compound from strawberry (Fragaria ananassa) leaves active against plant pathogens.
An antibiotic called fragarin showing activities against bacterial and fungal plant pathogens was isolated and purified by FPLC chromatography from the soluble fraction of strawberry leaves. The molecular weight value determined by mass spectrometry is 316 Da. Fragarin remains fully active after protease treatment or alkaline hydrolysis at 100 degrees C for 20 min. Biological and chemical analyses suggest that fragarin may be a new type of an antimicrobial preformed compound--phytoanticipin--and would constitute a primary non-specific barrier of strawberry defense.
Filippone
M P
MP
Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT), Chacabuco 461, (4000), San Miguel de Tucumán, Argentina.
Diaz Ricci
J
J
Mamaní de Marchese
A
A
Farías
R N
RN
Castagnaro
A
A
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002847:Chromatography, Agarose; D020231:Colletotrichum / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000737:chemistry; D011549:Pseudomonas / Q000187:drug effects; D019685:Rosales / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents