Lis1, the Drosophila homolog of a human lissencephaly disease gene, is required for germline cell division and oocyte differentiation.
Lissencephaly is a severe congenital brain malformation resulting from incomplete neuronal migration. One causal gene, LIS1, is homologous to nudF, a gene required for nuclear migration in A. nidulans. We have characterized the Drosophila homolog of LIS1 (Lis1) and show that Lis1 is essential for fly development. Analysis of ovarian Lis1 mutant clones demonstrates that Lis1 is required in the germline for synchronized germline cell division, fusome integrity and oocyte differentiation. Abnormal packaging of the cysts was observed in Lis1 mutant clones. Our results indicate that LIS1 is important for cell division and differentiation and the function of the membrane cytoskeleton. They support the notion that LIS1 functions with the dynein complex to regulate nuclear migration or cell migration.
Liu
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Z
The Waksman Institute, Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854-8020, USA.
Xie
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Steward
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D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000002:abnormalities*; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D004398:Dyneins / Q000235:genetics; D005260:Female; D017344:Genes, Insect; D006801:Humans; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D017931:DNA Primers; D029721:Drosophila Proteins; C513192:Lis-1 protein, Drosophila; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D011506:Proteins; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C084102:PAFAH1B1 protein, human; D004398:Dyneins