Epitopes for chicken monoclonal antibodies in spleens of selected Japanese quail lines.
A line of Japanese quail selected for high plasma cholesterol is highly susceptible to diet-induced atherosclerosis. Lymphocyte epitopes recognized by mouse anti-chicken monoclonal antibodies (c-mAb), TCR-1, TCR-2, TCR-3. CD-3, CD-4, CD-8, and BU-1a/b were reacted with spleens from quail selected for high (HL) and low (LL) plasma total cholesterol and their nonselected controls (CL). Cross reactivity to c-mAb and effect of line and gender were immunohistochemically evaluated. Chicken spleens were positive controls. Quail were immunologically stimulated with either sheep red blood cells (SRBC) or Brucella abortus 2 weeks before spleens were removed. Quail spleen epitopes of all lines recognized TCR-3 and CD-8 c-mAb, but no other c-mAb. Number of reacting cells and staining intensity to the TCR-3 c-mAb were greater in the HL than in the LL regardless of the stimulating Ag or dose used. For the CD-8 c-mAb, there were no differences among lines in birds receiving SRBC. In B. abortus-immunized birds, sex x line interactions indicated that males of the HL and CL had lower responses than females but LL males were not different than females. TCR-3 and CD8 c-mAb may be useful in studying immunological mechanisms for atherosclerosis in Japanese quail.
Lovitt
H L
HL
Department of Poultry Science, The Pennsylvania State University, University Park, USA.
Siegel
H S
HS
Weinstock
D
D
Marks
H L
HL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002003:Brucella abortus / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology / Q000378:metabolism; D002645:Chickens; D003370:Coturnix / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology* / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D008297:Male; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D012756:Sheep; D013154:Spleen / Q000166:cytology* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell