title: Quantification of telomerase activity by direct scintillation counting.
abstract: An improved telomerase assay was developed that allows direct quantification of the enzyme activity by scintillation counting of the labeled telomerase product. The assay measures the incorporation of 32P-dGTP into telomeric repeats synthesized at the 3' end of a biotinylated primer. Telomerase reaction product is separated from the reaction mix by streptavidin-coated magnetic beads and counted. The assay can be used for quantitative studies of human telomerase and its inhibitors.
authors.affiliation: Department of Oncology, Roche Research Center, Hoffmann-La Roche, Inc., Nutley, NJ 07110, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kazmer
authors.forename: K M
authors.initials: KM
authors.lastname: Pan
authors.forename: L
authors.initials: L
authors.lastname: Vassilev
mesh_terms: D001710:Biotin; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D010761:Phosphorus Radioisotopes; D012588:Scintillation Counting / Q000379:methods*; D019809:Streptavidin; D019098:Telomerase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000302:isolation & purification
chemical_list: D010761:Phosphorus Radioisotopes; D001710:Biotin; D019809:Streptavidin; D019098:Telomerase