Expression and function of cyclooxygenase-2 in mesothelial cells during late phase of rat carrageenin-induced pleurisy.
We have previously shown that the inducible isoform of the cyclooxygenases, COX-2, is strongly expressed in pleural exudate leukocytes early (between 3 and 7 hr after irritation) during rat carrageenin-induced pleurisy. The present study further examined COX-2 expression and disclosed that mesothelial cells expressed COX-2 later (12 to 24 hr after irritation) in this model. A COX-2 inhibitor, nimesulide, lowered the intrapleural level of 6-keto-PGF1alpha and inhibited hyperplasia of the pleural matrix, suggesting that COX-2 expressed in mesothelial cells may play a role in the synthesis of extracellular matrix through formation of PGI2.
Hatanaka
K
K
Department of Pharmacology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
Kawamura
M
M
Ogino
M
M
Saito
M
M
Ogino
K
K
Matsuo
S
S
Harada
Y
Y
D000818:Animals; D002351:Carrageenan; D051546:Cyclooxygenase 2; D004848:Epithelium / Q000201:enzymology; D011464:Epoprostenol / Q000378:metabolism; D007527:Isoenzymes / Q000502:physiology*; D008297:Male; D010998:Pleurisy / Q000139:chemically induced / Q000201:enzymology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D002351:Carrageenan; D011464:Epoprostenol; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases