title: [Retinal regeneration after dissection of the optic nerve in newts exposed on board the Bion-11 biosatellite].
abstract: The work brought up initial information on the impacts of space flight (SF) on regeneration of nerve tissues in vertebrata. Summarized are data of analysis of the retinal regeneration following section of the ocular nerve and blood vessels in space-flown adult newts (Pleurodeles waltlii). Two weeks in SF were found not to impede the regeneration of retina as its growth was fully dependent on the same cell sources as in the condition of 1 g. In the newts which had been operated 2 wk prior to launch, recovery of retina in SF proceeded more intensively (phases V-VI) compared with the synchronous controls (phase IV). According to the morphometric analysis, differentiation of regenerates' layers in the space animals was also a more rapid process. The proliferative activity of cells in regenerates estimated with the 3H-timidine radioautography turned to be higher, too: the labeled nuclei index in early non-differentiated regenerates was in 1.2 to 1.5 times higher than in the control. Immunohistochemical array with the help of GFAP antibodies performed at the late phases of regeneration revealed an activating effect of SF on the Muller glia cells. These findings indicate that microgravity can stimulate general retinal regeneration and activate regenerate cells, specifically those involved in morphogenesis.
authors.forename: E N
authors.initials: EN
authors.lastname: Grigorian
authors.forename: S Ia
authors.initials: SIa
authors.lastname: Tuchkova
authors.forename: V A
authors.initials: VA
authors.lastname: Poplinskaia
authors.forename: V I
authors.initials: VI
authors.lastname: Mitashov
mesh_terms: D000818:Animals; D001345:Autoradiography; D007150:Immunohistochemistry; D009416:Nerve Regeneration / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D010999:Pleurodeles / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000502:physiology*; D018531:Spacecraft; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
chemical_list: D014316:Tritium; D013936:Thymidine