Flow cytometric immunophenotyping in fine-needle aspiration of lymph nodes.
Fine-needle aspiration (FNA) of lymph nodes has been regarded as a useful method in the diagnosis of lymphadenopathy. However, this procedure has been shown to be of limited value in the diagnosis of low or intermediate grade malignant lymphomas in some studies. Immunophenotyping is an essential adjunct to cytomorphology for the diagnosis of lymphoma by FNA. Immunophenotyping using flow cytometry (FCM) is rapid, objective and reliable. Using FCM, multiparametric analysis of 33 FNA materials from lymph nodes was performed and profiles of surface markers of lymphoid cells were assessed. In reactive hyperplasia, patterns of cell surface markers were quite variable, but disclosed polyclonality. Most of the B-cell lymphomas showed immunophenotypes for B-cell lineages with their kappa: lambda or lambda: kappa ratio being over 3:1. In T-cell lymphomas, T-cell surface markers were predominantly expressed as well. In conclusion, our results suggest that immunophenotyping of lymph node aspirates is a valuable diagnostic adjunct for lymphoproliferative disorders, particularly in B-cell lymphomas because immunophenotyping can be easily and adequately performed by FCM.
Chung
J
J
Department of Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
Gong
G
G
Huh
J
J
Khang
S K
SK
Ro
J Y
JY
D018941:Antigens, CD19 / Q000032:analysis; D018951:Antigens, CD20 / Q000032:analysis; D019011:Antigens, CD7 / Q000032:analysis; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology; D001707:Biopsy, Needle; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D018956:CD5 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006689:Hodgkin Disease / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008206:Lymphatic Diseases / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology
D018941:Antigens, CD19; D018951:Antigens, CD20; D019011:Antigens, CD7; D017252:CD3 Complex; D015704:CD4 Antigens; D018956:CD5 Antigens; D016827:CD8 Antigens