title: Improved recovery of cryopreserved canine islets by use of beraprost sodium.
abstract: Cryopreservation of pancreatic islets provides many advantages for clinical transplantation. Unfortunately, the freezing and thawing processes lead to a significant loss of islets. In this study, an attempt was made to increase the yield and viability of islets after cryopreservation and thawing. By using canine islets, we evaluated whether beraprost sodium (BPS), a stable prostacyclin analog, protects islets during the freeze-thaw processes and improves the recovery of frozen-thawed islets. Canine islets were frozen and thawed by the procedures used routinely for storage of human islets. In this study, we deliberately used islets of lower purity (60+/-3.6%), which is undesirable for cryopreservation. The recovery of viable islets after thawing is poorer with islets of lower purity than with highly purified islets. BPS was added to both the cryopreservation solutions containing dimethyl sulfoxide (DMSO) and the thawing solution containing sucrose. After thawing, the islet recovery (islet number after thawing divided by islet number before freezing) was 71.1+/-12.7% with 1 nM BPS, 77.8+/-5.6% with 10 nM BPS, 79.3+/-6.7% with 100 nM BPS, and 69.2+/-7.2% in control preparations without BPS. Islet viability assessed by supravital staining was 57.5+/-5.6%, 64.7+/-7.0%, 67.5+/-6.5%, and 57.7+/-4.9% with 1 nM, 10 nM, and 100 nM BPS and controls, respectively. Both islet recovery and viability were significantly better with 10 nM and 100 nM BPS than with the controls (p<0.03). After 3 days in culture, islet numbers in the 10 nM and 100 nM BPS groups were significantly higher and showed better insulin-release responses than those from the 1 nM BPS and control groups. Histologically, islet structure was well preserved in the 10 nM and 100 nM BPS groups, whereas many islets of the control group were smaller and fragmented. Electron microscopic examination revealed that 10 nM and 100 nM BPS preserved the microstructure of islet cells, and signs of apoptosis or necrosis were rare. It was concluded that BPS improved the recovery and viability of canine islets after cryopreservation and thawing. BPS would be a useful agent for improving the recovery of cryopreserved human islets for clinical transplantation.
authors.affiliation: Department of Surgery, UCLA School of Medicine and Veterans Affairs Medical Center, West Los Angeles, California 90073, USA.
authors.forename: S
authors.initials: S
authors.lastname: Arita
authors.forename: A
authors.initials: A
authors.lastname: Kasraie
authors.forename: S
authors.initials: S
authors.lastname: Une
authors.forename: S
authors.initials: S
authors.lastname: Ohtsuka
authors.forename: T
authors.initials: T
authors.lastname: Kawahara
authors.forename: C V
authors.initials: CV
authors.lastname: Smith
authors.forename: Y
authors.initials: Y
authors.lastname: Mullen
mesh_terms: D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000378:metabolism; D004285:Dogs; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron
chemical_list: D003451:Cryoprotective Agents; D017077:Culture Media, Conditioned; D007328:Insulin; C048081:beraprost; D011464:Epoprostenol