Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species.
A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
Céspedes
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A
Universidad Complutense, Facultad de Veterinaria, Departmento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Madrid, Spain.
García
T
T
Carrera
E
E
González
I
I
Fernández
A
A
Hernández
P E
PE
Martín
R
R
D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005412:Flatfishes / Q000145:classification* / Q000235:genetics; D008928:Mitochondria / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial