Effect of dietary lipids on hepatic Na+,K(+)-ATPase in cyclosporine A-treated rats: immunocytochemical analysis of alpha1 subunit by confocal laser scanning microscopy imaging.
We studied the effect of dietary soybean lecithin or triacylglycerol on hepatic Na+,K(+)-ATPase in cyclosporine A-treated rats by means of quantitative immunocytochemistry. Cyclosporine A-treated rats were fed lecithin or a triacylglycerol-enriched diet or a low-fat diet. As a control, one group was only fed the low-fat diet; the three other groups were treated with cyclosporine A solvent and received the low fat, lecithin, or triacylglycerol diet. Bile canalicular staining significantly decreased in all cyclosporine A-treated groups with the higher values in lecithin-fed rats. In basolateral membranes, no decrease was observed in the lecithin-cyclosporine group, in contrast to the other groups. The triacylglycerol-cyclosporine group had lower values in both membrane domains. The alteration of Na+,K(+)-ATPase by cyclosporine A was related to cholestasis evidenced by a decrease in bile salt secretion. These modifications were prevented by dietary soybean lecithin and amplified by dietary soybean triacylglycerol.
Benkoël
L
L
Laboratoire de Pathologie Cellulaire et Métabolique du Foie (JE 2058), Faculté de Médecine, Marseille, France.
Chanussot
F
F
Dodero
F
F
de la Maisonneuve
C
C
Lambert
R
R
Brisse
J
J
Chamlian
A
A
D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000494:pharmacology*; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D010713:Phosphatidylcholines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014280:Triglycerides / Q000494:pharmacology
D001647:Bile Acids and Salts; D004041:Dietary Fats; D007166:Immunosuppressive Agents; D007527:Isoenzymes; D010713:Phosphatidylcholines; D014280:Triglycerides; D016572:Cyclosporine; D000254:Sodium-Potassium-Exchanging ATPase