The influence of the host cell on standardisation of influenza vaccine potency.
Conventional influenza vaccines are standardised using the single-radial-immunodiffusion (SRD) test where reagents are produced from egg-grown viruses. It is important to ensure homology between SRD antigen reagents and test vaccines. There was concern that cell-grown vaccines may differ antigenically from corresponding egg-grown vaccines, which may in turn affect vaccine standardisation. In an examination of five cell-grown vaccines from two companies, only one vaccine was affected by the specificity of the SRD test. Options for standardisation of cell-grown vaccines are considered and recommendations are made for further studies.
Wood
J M
JM
National Institute for Biological Standards and Control, Potters Bar, Herts, UK.
Dunleavy
U
U
Newman
R W
RW
Riley
A M
AM
Robertson
J S
JS
Minor
P D
PD
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002642:Chick Embryo; D004285:Dogs; D006801:Humans; D005779:Immunodiffusion; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology / Q000592:standards*; D011863:Radioimmunoassay; D014776:Virus Cultivation
D000956:Antigens, Viral; D007252:Influenza Vaccines