title: A distinctive autosomal dominant vacuolar neuromyopathy linked to 19p13.
abstract: OBJECTIVE
  To characterize a kindred with a distinctive autosomal dominant neuromuscular disorder.
  
  
  BACKGROUND
  The authors studied a large Italian family affected by a progressive neuromyopathy. Ten individuals over three generations were affected. The disease was characterized by onset from the late teens to early 50s with distal leg weakness and atrophy, development of generalized muscle weakness with distal-to-proximal progression sparing facial and ocular muscles, dysphonia and dysphagia, pes cavus and areflexia, variable clinical expression ranging from subclinical myopathy to severely disabling weakness, and mixed neurogenic and myopathic abnormalities on electromyography.
  
  
  METHODS
  Morphologic, immunocytochemical, and ultrastructural studies were performed in muscle biopsies from three affected patients. A genomewide linkage analysis through the genotyping of 292 microsatellite markers spanning the 22 autosomes was undertaken to map the disorder segregating in this family.
  
  
  RESULTS
  All muscle biopsies showed variation of fiber size, panesterase-positive angular fibers, mild to severe fibrosis, and numerous "rimmed vacuoles." Electron microscopy failed to demonstrate the nuclear or cytoplasmic filamentous inclusions specific of inclusion-body myopathies and, accordingly, immunohistochemistry did not show any positivity with SMI-31 antibodies detecting hyperphosphorylated tau. Preliminary analysis of 292 microsatellite markers provided evidence for linkage to chromosome 19p13.
  
  
  CONCLUSIONS
  This distinctive autosomal dominant disorder is characterized by a vacuolar neuromyopathy. Localization to chromosome 19p13 will allow the genetic relationship between this disease and inherited myopathies with rimmed vacuoles, in particular autosomal dominant inclusion-body myopathies, to be defined.
authors.affiliation: Institute of Neurology, Catholic University and U.I.L.D.M., Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Servidei
authors.forename: F
authors.initials: F
authors.lastname: Capon
authors.forename: A
authors.initials: A
authors.lastname: Spinazzola
authors.forename: M
authors.initials: M
authors.lastname: Mirabella
authors.forename: S
authors.initials: S
authors.lastname: Semprini
authors.forename: G
authors.initials: G
authors.lastname: de Rosa
authors.forename: M
authors.initials: M
authors.lastname: Gennarelli
authors.forename: F
authors.initials: F
authors.lastname: Sangiuolo
authors.forename: E
authors.initials: E
authors.lastname: Ricci
authors.forename: H W
authors.initials: HW
authors.lastname: Mohrenweiser
authors.forename: B
authors.initials: B
authors.lastname: Dallapiccola
authors.forename: P
authors.initials: P
authors.lastname: Tonali
authors.forename: G
authors.initials: G
authors.lastname: Novelli
mesh_terms: D000328:Adult; D000368:Aged; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D004576:Electromyography; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D006801:Humans; D007558:Italy; D008126:Lod Score; D008297:Male; D008875:Middle Aged; D009132:Muscles / Q000473:pathology / Q000503:physiopathology; D009468:Neuromuscular Diseases / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D010375:Pedigree; D014617:Vacuoles / Q000473:pathology*