A gated-beam electrospray ionization source with an external ion reservoir. A new tool for the characterization of biomolecules using electrospray ionization mass spectrometry.
In this work we describe a micro-electrospray ionization source equipped with an atmospheric pressure external ion shutter. The solenoid-activated shutter prevents the electrospray plume from entering the inlet capillary unless triggered to the 'open' position. When in the 'closed' position, a stable electrospray plume is maintained between the electrospray ionization (ESI) emitter and the electrically isolated face of the shutter. When the shutter is triggered, a 'slice' of ions is allowed to enter the inlet capillary and is subsequently accumulated in an external ion reservoir comprised of a radio frequency only (rf-only) hexapole and a pair of electrostatic elements. Following ion accumulation in the external ion reservoir, intact molecular ions of proteins, oligonucleotides, and noncovalent complexes can be stored for extended intervals (>30 minutes) prior to being transferred to the Fourier transform ion cyclotron resonance (FTICR) trapped ion cell for mass analysis. By introducing reactive gases directly into the external ion reservoir during the storage interval, ion-molecule reactions, such as H/D exchange, can be performed at high effective pressures. This scheme obviates the need for the long reaction times and delays associated with restoring base pressure in the trapped ion cell and allows H/D exchange reactions to be conducted in a fraction of the time required using conventional in-cell exchange approaches. The back face of the shutter arm contains an elastomeric material which can be positioned to seal the inlet to the mass spectrometer resulting in lower base pressure in the ion reservoir and the FTICR cell. Additionally, it is noted that blocking the ESI plume during non-accumulation events results in reduced fouling of the source electrodes and longer times between required source cleaning.
Hofstadler
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SA
Ibis Therapeutics, a Division of Isis Pharmaceuticals, 2292 Faraday Ave., Carlsbad, CA 92008, USA. shofstadler@isisph.com
Sannes-Lowery
K A
KA
Griffey
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RH
D000818:Animals; D003574:Cytochrome c Group / Q000737:chemistry; D006736:Horses; D007477:Ions; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D009211:Myoglobin / Q000737:chemistry; D011312:Pressure; D011506:Proteins / Q000737:chemistry*
D003574:Cytochrome c Group; D007477:Ions; D009211:Myoglobin; D011506:Proteins