title: The effect of cytokines on the proliferation and migration of bovine meniscal cells.
abstract: We determined the effect of cytokines on the proliferation and migration of cells isolated from the inner-third (white-white), middle-third (red-white), and outer-third (red-red) regions of bovine meniscus. Cells from the outer, or peripheral, region of the meniscus exhibited higher DNA synthesis in the presence of 10% serum compared with cells from the inner or central regions. Recombinant human platelet-derived growth factor-AB, hepatocyte growth factor/scatter factor, and bone morphogenic protein-2 stimulated DNA synthesis of all meniscal cells in a dose-dependent manner, with a two- to threefold maximal stimulation at 10 ng/ml. Cell migration was also stimulated by addition of cytokines. Platelet-derived growth factor and hepatocyte growth factor caused an increase in the migration of cells derived from all three zones, while interleukin-1 selectively stimulated the migration of outer-zone meniscal cells. Epidermal growth factor was much less effective and stimulated the migration of cells in the inner and outer zones by 40% to 50%, while bone morphogenic protein-2 and insulin-like growth factor-1 stimulated the migration of meniscal cells from the middle zone by 40% to 50%. The identification of cytokines that stimulate both the growth and migration of meniscal cells may provide new tools for modulation of meniscal healing.
authors.affiliation: Laboratory for Soft Tissue Research, Hospital for Special Surgery, New York, New York 10021, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Bhargava
authors.forename: E T
authors.initials: ET
authors.lastname: Attia
authors.forename: G A
authors.initials: GA
authors.lastname: Murrell
authors.forename: M M
authors.initials: MM
authors.lastname: Dolan
authors.forename: R F
authors.initials: RF
authors.lastname: Warren
authors.forename: J A
authors.initials: JA
authors.lastname: Hannafin
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D016207:Cytokines / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008592:Menisci, Tibial / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014316:Tritium
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016207:Cytokines; D007375:Interleukin-1; D010982:Platelet-Derived Growth Factor; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C095477:platelet-derived growth factor AB; C499469:recombinant human bone morphogenetic protein-2; D014316:Tritium; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D004247:DNA