Capillary electrochromatography of peptides in a microfabricated system.
Reversed-phase liquid chromatography of tryptic peptides is shown in the capillary electrochromatography mode using microfabricated columns. Although selectivity is different, a mixture of tryptic peptides from ovalbumin appears to be as easily separated in the CEC as HPLC mode. The major difference between a separation in the macrofabricated CEC column and conventional separations in the HPLC mode is that separations are more readily achieved in the isocratic mode in the lower surface area microfabricated CEC columns.
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Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Ji
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Regnier
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D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D010047:Ovalbumin / Q000032:analysis* / Q000378:metabolism; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism
D010455:Peptides; D010047:Ovalbumin; D014357:Trypsin