Epithelial cells of different organs exhibit distinct patterns of p53-dependent and p53-independent apoptosis following DNA insult.
The present study shows that DNA damage induces different patterns of p53-dependent and p53-independent apoptosis in epithelial cells of various organs of adult mice. Genotoxic stress induced a biphasic apoptotic response in the small intestine and tongue. While the first immediate apoptotic wave was p53-dependent, the second was slower in rate and was p53-independent. Under the same experimental conditions a single rapid, but a more extended, p53-independent response was evident in the skin of the tail. Indeed, exposure of p53+/+ mice to 400 R induced in epithelium of the small intestine and tongue an immediate rapid response that was followed by a second delayed p53-independent apoptotic wave. p53-/- mice exhibited in these organs the second wave only. However, epithelium of the tail derived from the same mice showed a single rapid apoptotic response that lasted much longer than the p53-dependent response and was similar in the p53-/- and the p53+/+ mice. Variations in apoptotic patterns observed in epithelial cells derived of the different tissues may point to differences in the physiological pathways expressed.
Uberti
D
D
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Schwartz
D
D
Almog
N
N
Goldfinger
N
N
Harmelin
A
A
Memo
M
M
Rotter
V
V
D000818:Animals; D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000528:radiation effects; D004249:DNA Damage; D017931:DNA Primers / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D016158:Genes, p53; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D018345:Mice, Knockout; D009928:Organ Specificity; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects
D017931:DNA Primers; D004247:DNA