9-[(2'S,3'S)-3'-formyl-2',3'-dihydroxypropyl]adenine: a facile affinity-labeling probe of human S-adenosyl-L-homocysteine hydrolase.
Treatment of human recombinant S-adenosyl-L-homocysteine (SAH) hydrolase with 9-[(2'S,3'S)-3'-formyl-2',3'-dihydroxypropyl]adenine (FDHPA) caused irreversible inactivation in a time- and concentration-dependent manner (Ki = 8.8 microM, k(inact) = 0.09 min(-1)). FDHPA behaved as a facile affinity-labeling probe of SAH hydrolase.
Kitade
Y
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Japan.
Nakanishi
M
M
Yatome
C
C
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D043383:Adenosylhomocysteinase; D000345:Affinity Labels; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
C122097:9-(3'-formyl-2,'3'-dihydroxypropyl)adenine; D000345:Affinity Labels; D011994:Recombinant Proteins; D006867:Hydrolases; D043383:Adenosylhomocysteinase; D000225:Adenine