Photoreceptor layer reconstruction in a rodent model of retinal degeneration.
We have examined the potential of retinal cell transplantation to dystrophic retinal degeneration mice as a way of replacing photoreceptors lost because of an intrinsic genetic defect. Early postnatal retinae which had been gently dissociated survived for at least 6 weeks after transplantation to the subretinal space. Over a significant area of distribution, transplanted cells formed outer segments which lay in close apposition to the host retinal pigment epithelial cell layer. The grafts integrated with the remaining host retina, sufficient at least to mediate a simple light-dark preference. A new synaptic layer was seen at the graft-host interface, which contained substantial numbers of photoreceptor synapses. This and the fact that the behavior could be elicited at low luminance levels argue for functional circuit reconstruction between grafted cells and host retina.
Kwan
A S
AS
Neural Transplant Program Department of Pathology, Institute of Ophthalmology, University College London, Bath Street, London, EC1V 9EL, United Kingdom.
Wang
S
S
Lund
R D
RD
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D003624:Darkness; D004195:Disease Models, Animal; D005808:Genes, Recessive; D006085:Graft Survival; D008029:Lighting; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009154:Mutation; D010727:Phosphoric Diester Hydrolases; D012160:Retina / Q000601:surgery; D017948:Retinal Rod Photoreceptor Cells / Q000201:enzymology / Q000637:transplantation* / Q000648:ultrastructure; D012174:Retinitis Pigmentosa / Q000235:genetics / Q000628:therapy*; D014785:Vision, Ocular
D010727:Phosphoric Diester Hydrolases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; C517438:Pde6b protein, mouse