Optimization in hybridoma cell culture.
Based on the formation kinetics of monoclonal antibody, the bench-scale culture of hybridoma cells in a bioreactor was optimized by the application of perfusion culture mode, the supplement of potassium acetate, and the fortification of nutrients. When the bioreactor was operated with the daily perfusion of medium of 1/2 working volume, viable cell density in bioreactor was 11 x 10(5) cells/ml and antibody concentration 28 mg/L. After supplement with 1 g/L potassium acetate, the cell density kept constant and antibody production was improved to 38 mg/L. The fortification of nutrients such as amino acids and vitamins raised the cell density up to 42 x 10(5) cells/ml and, responsively, antibody concentration to 94 mg/L.
Zhang
Y
Y
State Key Laboratory of Bioreactor Engineering, East China University of Science and Biotechnology, Shanghai, China.
Fong
W
W
Yung
P
P
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured