Frozen allogeneic human epidermal cultured sheets for the cure of complicated leg ulcers.
BACKGROUND
Skin ulcers due to venous stasis or diabetes are common among the elderly and are difficult to treat. Repeated applications of cell-based products have been reported to result in cure or improvement of leg ulcers of small size in a fraction of patients.


OBJECTIVE
To examine the effects of frozen human allogeneic epidermal cultures for the treatment of acute and chronic ulcers.


METHODS
We treated a series of 10 consecutive patients with leg ulcers of different etiology and duration with frozen human allogeneic epidermal cultures stored frozen and thawed for 5-10 minutes at room temperature before application. Three patients had ulcers with exposed Achilles or extensor tendon. The ulcers treated were as large as 160 cm2 in area and of up to 20-years' duration. After preliminary preparation of the wounds by debridement to remove necrotic tissue and application of silver sulfadiazine to control infection, thawed cultures were applied biweekly from 2 to 15 times depending on the size and complexity of the ulcer.


RESULTS
All ulcers healed, including those with tendon exposure. After the first few applications, granulation tissue formed in the ulcer bed and on exposed tendons, and epidermal healing took place through proliferation and migration of cells from the margins of the wound. The time required for complete healing ranged from 1 to 31 weeks after the first application.


CONCLUSIONS
The use of frozen human allogeneic epidermal cultures is a safe and effective treatment for venous or diabetic ulcers, even those with tendon exposure. It seems possible that any leg ulcer will be amenable to successful treatment by this method.
Bolívar-Flores
Y J
YJ
Hospital Médica Sur, Department of Cell Biology, Centro de Investigación y Estudios Avanzados delIPN, Mexico City, Mexico.
Kuri-Harcuch
W
W
D016475:3T3 Cells / Q000637:transplantation; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002460:Cell Line; D015925:Cryopreservation; D046508:Culture Techniques; D005260:Female; D016523:Foot Ulcer / Q000601:surgery; D006801:Humans; D007871:Leg Ulcer / Q000601:surgery*; D008297:Male; D051379:Mice; D008875:Middle Aged; D011183:Postoperative Complications / Q000503:physiopathology; D016038:Skin Transplantation / Q000379:methods*; D014184:Transplantation, Homologous; D014647:Varicose Ulcer / Q000601:surgery; D014945:Wound Healing / Q000502:physiology