title: Solution structure of the ribosomal protein S19 from Thermus thermophilus.
abstract: Ribosomal protein S19 is a 10.6 kDa protein in the small subunit of the prokaryotic ribosome. We have determined a high-resolution solution structure of S19 from Thermus thermophilus. Structures were calculated using 1160 distance and dihedral angle restraints derived from (1)H, (15)N and (13)C NMR spectra. The structures show that S19 is a mixed alpha/beta protein with long disordered tails. The folding topology is not homologous to that of any other known protein structure. Potential rRNA and protein binding sites have been identified on the S19 surface.
authors.affiliation: Department of Biotechnology Royal Institute of Technology (KTH), Center for Structural Biochemistry, Novum, Huddinge, S-141 57, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Helgstrand
authors.forename: A V
authors.initials: AV
authors.lastname: Rak
authors.forename: P
authors.initials: P
authors.lastname: Allard
authors.forename: N
authors.initials: N
authors.lastname: Davydova
authors.forename: M B
authors.initials: MB
authors.lastname: Garber
authors.forename: T
authors.initials: T
authors.lastname: Härd
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D012335:RNA, Ribosomal / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions; D055672:Static Electricity; D013696:Temperature; D016963:Thermus thermophilus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; D012996:Solutions; C040343:ribosomal protein S19