Critical role of CD23 in allergen-induced bronchoconstriction in a murine model of allergic asthma.
CD23-deficient and anti-CD23 monoclonal antibody-treated mice were used to investigate the role of the low-affinity receptor for IgE (CD23) in allergic airway inflammation and airway hyperresponsiveness (AHR). While there were no significant differences in ovalbumin (OVA)-specific IgE titers and tissue eosinophilia, evaluation of lung function demonstrated that CD23-/- mice showed an increased AHR to methacholine (MCh) when compared to wild-type mice but were completely resistant to the OVA challenge. Anti-CD23 Fab fragment treatment of wild-type mice did not affect the MCh-induced AHR but significantly reduced the OVA-induced airway constriction. These results imply a novel role for CD23 in lung inflammation and suggest that anti-CD23 Fab fragment treatment may be of therapeutic use in allergic asthma.
Dasic
G
G
Department of Immunology Geneva Biomedical Research Institute, Glaxo Wellcome Research and Development S.A., Geneva, Switzerland.
Juillard
P
P
Graber
P
P
Herren
S
S
Angell
T
T
Knowles
R
R
Bonnefoy
J Y
JY
Kosco-Vilbois
M H
MH
Chvatchko
Y
Y
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology*; D016084:Bronchoconstriction / Q000276:immunology*; D016085:Bronchoconstrictor Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis; D016676:Macrophages, Alveolar / Q000378:metabolism; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE / Q000172:deficiency / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D000485:Allergens; D016085:Bronchoconstrictor Agents; D011961:Receptors, Fc; D017455:Receptors, IgE; D016210:Methacholine Chloride; D007073:Immunoglobulin E; D010047:Ovalbumin