Construction of engineering host by non-random method in Saccharomyces cerevisiae.
A yeast engineering host, GJ30, was constructed by a non-random method. GJ30 derivated from wild type yeast have leu2- and ura3- deficients which can be used as selection markers. The PCR analysis showed that the homologous recombinations occurred in the leu2 and ura3 locus of GJ30, respectively. The biological properties (cellular growth density, stability and efficiency for gene expression) of GJ30 were compared with other several yeast stains. The results showed that GJ30 is a suitable host strain for expression of foreign genes.
Gong
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Y
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, China.
Chen
Y
Y
Yang
S
S
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase