title: The monoclonal antibody DCGM4 recognizes Langerin, a protein specific of Langerhans cells, and is rapidly internalized from the cell surface.
abstract: We generated monoclonal antibody (mAb) DCGM4 by immunization with human dendritic cells (DC) from CD34+ progenitors cultured with granulocyte-macrophage colony-stimulating factor and TNF-alpha. mAb DCGM4 was selected for its reactivity with a cell surface epitope present only on a subset of DC. Reactivity was strongly enhanced by the Langerhans cell (LC) differentiation factor TGF-beta and down-regulated by CD40 ligation. mAb DCGM4 selectively stained LC, hence we propose that the antigen be termed Langerin. mAb DCGM4 also stained intracytoplasmically, but neither colocalized with MHC class II nor with lysosomal LAMP-1 markers. Notably, mAb DCGM4 was rapidly internalized at 37 degrees C, but did not gain access to MHC class II compartments. Finally, Langerin was immunoprecipitated as a 40-kDa protein with a pI of 5.2 - 5.5. mAb DCGM4 will be useful to further characterize Langerin, an LC-restricted molecule involved in routing of cell surface material in immature DC.
authors.affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
authors.forename: J
authors.initials: J
authors.lastname: Valladeau
authors.forename: V
authors.initials: V
authors.lastname: Duvert-Frances
authors.forename: J J
authors.initials: JJ
authors.lastname: Pin
authors.forename: C
authors.initials: C
authors.lastname: Dezutter-Dambuyant
authors.forename: C
authors.initials: C
authors.lastname: Vincent
authors.forename: C
authors.initials: C
authors.lastname: Massacrier
authors.forename: J
authors.initials: J
authors.lastname: Vincent
authors.forename: K
authors.initials: K
authors.lastname: Yoneda
authors.forename: J
authors.initials: J
authors.lastname: Banchereau
authors.forename: C
authors.initials: C
authors.lastname: Caux
authors.forename: J
authors.initials: J
authors.lastname: Davoust
authors.forename: S
authors.initials: S
authors.lastname: Saeland
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D019013:CD40 Antigens / Q000378:metabolism; D023201:CD40 Ligand; D002469:Cell Separation; D015536:Down-Regulation / Q000276:immunology; D006801:Humans; D007801:Langerhans Cells / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D037181:Lectins, C-Type; D008024:Ligands; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; C121916:CD207 protein, human; D019013:CD40 Antigens; D037181:Lectins, C-Type; D008024:Ligands; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D023201:CD40 Ligand