title: The recombinant 5-HT1A receptor: G protein coupling and signalling pathways.
abstract: The 5-hydroxytryptamine 5-HT1A receptor was one of the first G protein coupled receptors whose cDNA and gene were isolated by molecular cloning methods. Transfection of the cDNA of this receptor into cells previously bearing no 5-HT receptors has resulted in the acquisition of large amounts of information regarding potential signal transduction pathways linked to the receptor, correlations of receptor structure to its various functions, and pharmacological properties of the receptor. Transfection studies with the 5-HT1A receptor have generated critical new information that might otherwise have been elusive. This information notably includes the discovery of unsuspected novel signalling linkages, the elucidation of the mechanisms of receptor desensitization, the refinement of models of the receptor pharmacophore, and the development of silent receptor antagonists, among others. The current review summarizes the most important studies of the recombinant 5-HT1A receptor in the decade since the identification of its cDNA.
authors.affiliation: Division of Nephrology, Department of Medicine, Medical University of South Carolina and the Ralph H. Johnson Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA. raymondj@musc.edu
authors.forename: J R
authors.initials: JR
authors.lastname: Raymond
authors.forename: Y V
authors.initials: YV
authors.lastname: Mukhin
authors.forename: T W
authors.initials: TW
authors.lastname: Gettys
authors.forename: M N
authors.initials: MN
authors.lastname: Garnovskaya
mesh_terms: D000483:Alleles; D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011985:Receptors, Serotonin / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D044263:Receptors, Serotonin, 5-HT1; D015290:Second Messenger Systems / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection / Q000502:physiology*
chemical_list: D011985:Receptors, Serotonin; D044263:Receptors, Serotonin, 5-HT1; D019204:GTP-Binding Proteins