title: A new mass screening method for methylmercury poisoning using mercury-volatilizing bacteria from Minamata Bay.
abstract: A simplified mass screening method for methylmercury exposure was developed using methylmercury-volatilizing bacteria from Minamata Bay. Some bacteria can transform methylmercury into mercury vapor. Most mercury in the hair is methylmercury, which is readily extracted with HCl solution. Black spots are formed on X-ray film due to the reduction of Ag(+) emulsion with mercury vapor produced by methylmercury-volatilizing bacteria. By exploiting these characteristics, a screening method was developed, whereby the fur of rats injected with methylmercury chloride formed clear black spots on X-ray film, whereas the fur of rats injected with saline did not. Subsequently, 50 human hair samples were examined using this mass screening method. The method identified people who had high mercury concentration, over 20 microg/g. A few thousand hair samples may be screened in a day using this method because it is rapid, simple, and economical. This method, therefore, enables screening of persons with methylmercury poisoning in mercury-polluted areas.
authors.affiliation: National Institute for Minamata Disease, Minamata, 4058-18 Hama, Kumamoto, 867-0008, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: I
authors.initials: I
authors.lastname: Naruse
authors.forename: Y
authors.initials: Y
authors.lastname: Takizawa
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods; D006197:Hair / Q000737:chemistry; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008628:Mercury / Q000378:metabolism; D008767:Methylmercury Compounds / Q000032:analysis / Q000378:metabolism / Q000506:poisoning*; D011634:Public Health; D051381:Rats; D014835:Volatilization; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014962:X-Ray Film
chemical_list: D008767:Methylmercury Compounds; D014874:Water Pollutants, Chemical; D008628:Mercury