title: N-linked oligosaccharides can protect target cells from the lysis mediated by NK cells but not by cytotoxic T lymphocytes: role of NKG2-A.
abstract: We have previously shown that glycophorin A (GPA), inserted by electropulsation into the membrane of K562 cells, protected them from natural killer (NK) cell-mediated cytotoxicity and the unique N-linked oligosaccharide of GPA was essential for resistance to occur. The present study demonstrates that the protection level conferred by GPA is similar to the resistance induced by HLA-Cw3 expressed by transfected K562 cells. A monoclonal antibody against NKG2-A, an NK inhibitory receptor interacting with HLA class I antigens and belonging to the C-type lectin receptor, was able to restore the ability of NK cells to lyse K562 cells expressing HLA-Cw3 at the cell membrane but not electroinserted-GPA, suggesting that the N-linked oligosaccharide of GPA cannot be a ligand for NKG2-A. GPA was then electroinserted into the membrane of two lymphoblastoid B-cell lines: one was sensitive to NK cell-mediated lysis, the other was susceptible to cytotoxic CD8+ T-lymphocyte (CTL)-mediated cytotoxicity. The electroinserted GPA protected the target cells from NK-mediated cytotoxicity, whereas it did not modify the cell susceptibility to lysis by CTL. Endoglycosidase F treatment abolished the resistance towards NK cell-mediated lysis, suggesting that N-linked glycans could inhibit mechanisms used by NK cells to exert their cytotoxic function in agreement with our previous results.
authors.affiliation: INSERM U 466, Institut Louis Bugnard, CHU Ranguell, Toulouse, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Sol
authors.forename: N
authors.initials: N
authors.lastname: Vacaresse
authors.forename: J
authors.initials: J
authors.lastname: Lule
authors.forename: C
authors.initials: C
authors.lastname: Davrinche
authors.forename: B
authors.initials: B
authors.lastname: Gabriel
authors.forename: J
authors.initials: J
authors.lastname: Teissie
authors.forename: A
authors.initials: A
authors.lastname: Ziegler
authors.forename: M
authors.initials: M
authors.lastname: Thomsen
authors.forename: H
authors.initials: H
authors.lastname: Benoist
mesh_terms: D002236:Carbohydrate Conformation; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D006021:Glycophorins / Q000378:metabolism / Q000502:physiology; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology*; D055654:NK Cell Lectin-Like Receptor Subfamily C; D009844:Oligosaccharides / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism / Q000502:physiology*; D055607:Receptors, Natural Killer Cell; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D006021:Glycophorins; C527054:KLRC1 protein, human; D055654:NK Cell Lectin-Like Receptor Subfamily C; D009844:Oligosaccharides; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell