[Preparation of Drosophila mitotic chromosomes for electron-microscopic studies].
A method of chromosome spreading on microscopic slides was modified for electron microscopy of metaphase chromosomes in Drosophila tissues. The slides covered with an electron transparent film were plasmochemically modified to make them hydrophilic. A piece of fixed tissue was macerated in 60% propionic acid before spreading chromosomes over the slide. The parts of preparation selected under light microscope for electron microscopic examination were cut and peeled of the slide to the top of a water drop. It was shown that the resolution of chromosomal structures was significantly higher than seen under optical microscope, but lower than in serial sections.
Galieva
E R
ER
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk.
Gruzdev
A D
AD
Petrov
A K
AK
Omel'ianchuk
L V
LV
D000818:Animals; D002875:Chromosomes; D004330:Drosophila / Q000235:genetics*; D007814:Larva; D008677:Metaphase; D008854:Microscopy, Electron; D008938:Mitosis