title: Highly-sensitive micellar electrokinetic chromatographic analysis of dioxin-related compounds using on-line concentration.
abstract: An application study of an on-line concentration technique of neutral analytes for micellar electrokinetic chromatography (MEKC) was carried out in environmental analysis to enhance the UV detection sensitivity. Several dioxins and related compounds, such as dibenzofuran, dibenzo-p-dioxin, 2,3- and 2,7-dichlorodibenzo-p-dioxins, and 2,3,7-trichlorodibenzo-p-dioxin, were used as test solutes. For a highly sensitive separation and detection, cyclodextrin-modified MEKC (CD-MEKC) under acidic conditions was employed as a separation mode and stacking using reverse migrating micelles and a water plug (SRW) as an on-line concentration technique. Almost a 200-fold gain in detection sensitivity was obtained for the model compounds in SRW-CD-MEKC compared to that in normal CD-MEKC without on-line concentration and the limit of detection was found to be around 0.1 ppm for each solute.
authors.affiliation: Department of Material Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan. otsuka@sci.himeji-tech.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Otsuka
authors.forename: H
authors.initials: H
authors.lastname: Hayashibara
authors.forename: S
authors.initials: S
authors.lastname: Yamauchi
authors.forename: J P
authors.initials: JP
authors.lastname: Quirino
authors.forename: S
authors.initials: S
authors.lastname: Terabe
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D004147:Dioxins / Q000032:analysis*; D009862:Online Systems; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis
chemical_list: D003505:Cyclodextrins; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants