title: Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.
abstract: It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small 2hJHC' interactions in the presence of the much larger through-covalent-bond 2JHC' and 3JHC' couplings. The ratio of the observed through-H-bond correlation intensity and the 2JHC' connectivity observed in a reference spectrum indicates 2hJHC' values of ca. 0.4-0.6 Hz, which are only slightly smaller than the corresponding 3hJNC' values. However, for technical reasons, 2hJHC' couplings are more difficult to measure than 3hJNC'.
authors.affiliation: Institute of Structural Biology, Forschungszentrum Jülich, Jülich, 52425, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Cordier
authors.forename: M
authors.initials: M
authors.lastname: Rogowski
authors.forename: S
authors.initials: S
authors.lastname: Grzesiek
authors.forename: A
authors.initials: A
authors.lastname: Bax
mesh_terms: D000577:Amides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D003903:Deuterium; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011522:Protons; D014452:Ubiquitins / Q000737:chemistry*
chemical_list: D000577:Amides; D000596:Amino Acids; D011522:Protons; D014452:Ubiquitins; D002244:Carbon; D003903:Deuterium