Liposomes as vehicles for the presentation of a synthetic peptide containing an epitope of hepatitis A virus.
Previous work from our group showed that the entrapment of HAV-related synthetic peptides into multilamellar liposomes yielded satisfactory immunoresponses when administered to mice. In the present work we report investigative results for several liposome formulations of a 20-mer peptide related to VP3 capsid protein of HAV. In this sense, the recently introduced surface plasmon resonance technique has been applied to compare the different strategies of association between the synthetic peptide and phospholipid vesicles and to demonstrate that no significant alteration in antigenicity is produced when the peptide sequence is covalently coupled to the surface of small unilamellar vesicles. In addition, conformational data obtained by the circular dichroism technique have shown a decrease in the helical contribution of peptide-once linked to phospholipid; probably this change is due to the restriction introduced at the N-terminus of the sequence when coupled to the derivatized phospholipids at the surface of vesicle bilayers.
García
M
M
Departament de Química de Pèptids i Proteïnes, CID, CSIC, Jordi Girona, 18-26, 08034, Barcelona, Spain.
Alsina
M A
MA
Reig
F
F
Haro
I
I
D000818:Animals; D002942:Circular Dichroism; D004337:Drug Carriers; D000939:Epitopes; D018963:Hepatitis Antigens / Q000276:immunology*; D006507:Hepatovirus / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D010455:Peptides / Q000276:immunology*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D020539:Sequence Analysis, Protein; D014614:Vaccines, Synthetic / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D004337:Drug Carriers; D000939:Epitopes; D018963:Hepatitis Antigens; D008081:Liposomes; D010455:Peptides; D014614:Vaccines, Synthetic; D014764:Viral Proteins