title: Experimental inoculation of avian polyomavirus in chemically and virally immunosuppressed chickens.
abstract: The purpose of this series of experiments was to determine the effect of various types of immunosuppressive treatments (cyclophosphamide, infectious bursal disease virus [IBDV], chicken anemia virus [CAV], and combination infection with IBDV and CAV) on susceptibility of chickens to challenge with avian polyomavirus. In the first experiment, chickens were chemically bursectomized with intraperitoneal injections of cyclophosphamide; in the second study, chickens were orally inoculated with IBDV; in the third study, birds were intramuscularly inoculated with CAV; and in the final study, birds were inoculated with both IBDV and CAV. In all experiments, chickens were challenged with 10(4.7) tissue culture infective doses of polyomavirus intraperitoneally. Only chemically bursectomized chickens developed lesions similar to those found in the naturally occurring multisystemic fatal form of polyomavirus infection seen in psittacine nestlings, including hepatic necrosis and large pale intranuclear inclusions.
authors.affiliation: Avian Disease Research Laboratory, Animal Health Diagnostic Laboratory, Michigan State University, Lansing 48909, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Fitzgerald
authors.forename: S J
authors.initials: SJ
authors.lastname: Kingwill
authors.forename: S
authors.initials: S
authors.lastname: Briggs
authors.forename: O
authors.initials: O
authors.lastname: Awolaja
authors.forename: A
authors.initials: A
authors.lastname: Basile
authors.forename: L
authors.initials: L
authors.lastname: Griffioen
authors.forename: E A
authors.initials: EA
authors.lastname: Potter
authors.forename: C C
authors.initials: CC
authors.lastname: Wu
authors.forename: S P
authors.initials: SP
authors.lastname: Taylor
authors.forename: W M
authors.initials: WM
authors.lastname: Reed
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D002060:Bursa of Fabricius / Q000276:immunology; D017727:Chicken anemia virus / Q000276:immunology*; D002645:Chickens; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D003520:Cyclophosphamide / Q000494:pharmacology*; D007165:Immunosuppression Therapy; D007243:Infectious bursal disease virus / Q000276:immunology*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009929:Organ Size; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013997:Time Factors; D017778:Vaccines, Combined; D014765:Viral Vaccines
chemical_list: D017778:Vaccines, Combined; D014765:Viral Vaccines; D003520:Cyclophosphamide