title: Separation of carbonyl 2,4-dinitrophenylhydrazones by capillary electrochromatography with diode array detection.
abstract: The applicability of capillary electrochromatography (CEC) with photodiode array detection for the analysis of carbonyl hydrazones is presented. The CEC separation of thirteen hydrazones was optimized by a systematic variation of conditions using a commercially available CE system and a 3-micron porous C18-bonded silica capillary column. The separation profile obtained under optimal isocratic conditions (60% actonitrile-4% tetrahydrofuran-5 mM Tris, pH 8) is similar to those reported for gradient HPLC, with significant improvements in efficiency (to 150,000-250,000 theoretical plates/m) and analysis time (by a factor of 4). The retention time reproducibility is better than 0.2% (RSD) from run to run and 1% from day to day. The limits of detection for individual carbonyl hydrazones range between 0.1 and 0.5 microgram/ml. Applications to ambient air and automobile exhaust are shown.
authors.affiliation: Analysis and Air Quality Division, Environmental Technology Centre, Environmental Canada, Ottawa, ON, Canada. dabek.ewa@etc.ec.gc.ca
authors.forename: E
authors.initials: E
authors.lastname: Dabek-Zlotorzynska
authors.forename: E P
authors.initials: EP
authors.lastname: Lai
mesh_terms: D000388:Air / Q000032:analysis; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D004784:Environmental Monitoring; D006835:Hydrazones / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D001335:Vehicle Emissions / Q000032:analysis
chemical_list: C001072:2,4-dinitrophenylhydrazone; D002021:Buffers; D006835:Hydrazones; D012997:Solvents; D001335:Vehicle Emissions