Glutelin basic subunits have a mammalian mucin-type O-linked disaccharide side chain.
Characterization was done on the sugar chains of glutelin, a major storage protein in rice seeds. The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A (Con A) and peanut agglutinin (PNA). The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4, NaOH, or O-glycanase. The sugar chains of the subunits, obtained by hydrazinolysis and O-glycanase, were pyridylaminated and subjected to 2-dimensional HPLC analysis using C(18) and acrylamide-derivatized columns. It was found that the Galbeta-1,3GalNAc disaccharide, which was previously identified as a core 1 structure of mucin-type sugar chains, is conjugated to the glutelin subunits. Furthermore, amino acid sequencing of an 11-kDa peptide of the subunits, recognized by both PNA and Con A, suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits.
Kishimoto
T
T
Graduate School of Natural Science and Technology, Niigata University, Ikarashi 2-Nocho, Niigata, 950-8101, Japan.
Watanabe
M
M
Mitsui
T
T
Hori
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A; D004187:Disaccharides / Q000737:chemistry; D005983:Glutens / Q000737:chemistry* / Q000235:genetics; D006031:Glycosylation; D008322:Mammals; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D012275:Oryza / Q000737:chemistry / Q000235:genetics; D019887:Peanut Agglutinin; D010449:Peptide Mapping; D037121:Plant Lectins
D004187:Disaccharides; D009077:Mucins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D003208:Concanavalin A; D005983:Glutens