Effect of deregulated IL-7 transgene expression on B lymphocyte development in mice expressing mutated pre-B cell receptors.
Deregulated overexpression of IL-7 under the control of the promoter of the Ealpha gene of MHC class II in IL-7-transgenic mice changes B cell development in wild-type mice and in mutants which limit B cell development at various cellular stages. While the introduction of deregulated IL-7 production does not change the size of the pro-B and pre-B I compartments in the bone marrow of wild-type and lambda5-/- mice, it increases these compartments 2.5- to fivefold in mice which cannot make immature and mature B cells, i. e. in RAG-2-/-, tmmuH-/-, and RAG-2-/- mice expressing a transgenic muH chain. Excessive IL-7 production also increases four- to fivefold the pre-B II compartment in all those mouse strains where it can be formed (i. e. in wild-type, lambda5-/- and muH chain-transgenic RAG-2-/- mice), while no pre-B- II-like cells appear in excessively IL-7-stimulated bone marrow of mice devoid of pre-B II cells (i. e. in tmmuH-/- and RAG-2-/- mice). In the spleen of all IL-7-transgenic mice significant numbers of both pro-B and pre-B I cells are detectable and increased numbers of pre-B II and immature B cells appear in the spleen of mouse strains which are capable of making them. The capacity of the spleen to accommodate expanded numbers of these B-lineage cells as well as mature B cells is much larger than that of the bone marrow of the IL-7-transgenic mice probably because the bone limits cellular expansion and provokes spillover into the peripheral lymphoid organs.
Ceredig
R
R
The Basel Institute for Immunology, Basel, Switzerland.
Andersson
J
J
Melchers
F
F
Rolink
A
A
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000276:immunology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000276:immunology; D015851:Interleukin-7 / Q000235:genetics* / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics* / Q000276:immunology; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D019076:Transgenes / Q000276:immunology*; D019895:Transposases / Q000276:immunology
D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains; D015851:Interleukin-7; D011498:Protein Precursors; C499308:Rag2 protein, mouse; D011947:Receptors, Antigen, B-Cell; C064655:V(D)J recombination activating protein 2; D019895:Transposases; D017493:Leukocyte Common Antigens