title: Early steps of replication of moloney murine leukemia virus in resting lymphocytes.
abstract: We explored the role of cell type in the early steps of replication of Moloney murine leukemia virus (Mo-MLV) by comparing viral entry and reverse transcription in physiologically quiescent peripheral blood B and T lymphocytes. Virus entry was identical in both cell types. In contrast to previous results, full-length viral DNA was synthesized in resting B lymphocytes, but in agreement with earlier reports, reverse transcription was abortive in resting T lymphocytes. The addition of exogenous nucleosides in the culture medium of resting T lymphocytes allowed reverse transcription to proceed in these cells, without inducing cell cycling. These data suggest that the difference in the ability of quiescent T and B lymphocytes to sustain reverse transcription of Mo-MLV can be explained by a difference in the dNTP pool sizes of these two populations of quiescent cells.
authors.affiliation: Laboratoire d'Hématologie Cellulaire et Moléculaire, Faculté de Pharmacie-Paris V, 4 Avenue de I'Observatoire, 75006, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Piéroni
authors.forename: P
authors.initials: P
authors.lastname: Bouillé
authors.forename: C
authors.initials: C
authors.lastname: Auclair
authors.forename: J J
authors.initials: JJ
authors.lastname: Guillosson
authors.forename: J
authors.initials: J
authors.lastname: Nafziger
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000821:virology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D016679:Genome, Viral; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D008297:Male; D051379:Mice; D008979:Moloney murine leukemia virus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D009705:Nucleosides / Q000378:metabolism / Q000494:pharmacology; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
chemical_list: D004279:DNA, Viral; D009705:Nucleosides; D012367:RNA, Viral