title: Tissue engineering of biphasic joint cartilage transplants.
abstract: In isolated posttraumatic or idiopathic joint defects the chondral layers and adjacent subchondral spongy bone are usually destructed. For regeneration we suggest the in vitro formation of a cartilage-coated biomaterial carriers (biphases) in order to fill the correspondingjoint defects. In this study Biocoral, a natural coralline material made of calcium carbonate, and calcite, a synthetic calcium carbonate, were used as supports for the cultivation of bovine chondrocytes in a three-dimensional polymer fleece. The cell-polymer-structure was affixed to the biomaterial with a fibrin-cell-solution. The artificial cartilage formed a new matrix and fused with the underlying biomaterial. The results indicate a promising technical approach to anchor tissue engineered cartilage in joint defects.
authors.affiliation: Department of Traumatology and Reconstructive Surgery, University Medical Center Benjamin Franklin, Free University of Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Kreklau
authors.forename: M
authors.initials: M
authors.lastname: Sittinger
authors.forename: M B
authors.initials: MB
authors.lastname: Mensing
authors.forename: C
authors.initials: C
authors.lastname: Voigt
authors.forename: G
authors.initials: G
authors.lastname: Berger
authors.forename: G R
authors.initials: GR
authors.lastname: Burmester
authors.forename: R
authors.initials: R
authors.lastname: Rahmanzadeh
authors.forename: U
authors.initials: U
authors.lastname: Gross
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D002119:Calcium Carbonate; D002356:Cartilage / Q000637:transplantation*; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes; D019737:Transplants
chemical_list: D001672:Biocompatible Materials; D002119:Calcium Carbonate