title: Escherichia coli binding to and invasion of brain microvascular endothelial cells derived from humans and rats of different ages.
abstract: Escherichia coli meningitis commonly occurs in the neonatal period, but the basis of this age dependency is unclear. We have previously identified two types of E. coli-brain microvascular endothelial cell (BMEC) interactions contributing to E. coli traversal of the blood-brain barrier (i.e., binding and invasion). The present study examined whether the age dependency of E. coli meningitis stemmed from differences in the capacities of neonatal and adult BMECs to interact with E. coli. BMECs were isolated from rats of different ages (10 days, 20 days and 3 months) as well as from humans of different ages (fetuses, 4- to 7-year-old children, and a 35-year-old adult, and 60- to 85-year-old geriatrics). The bindings of E. coli to young and old rat BMECs were similar. Also, the abilities of E. coli to invade BMECs were similar for BMECs derived from young and old rats and from human fetuses, children, adults, and geriatrics. These findings suggest that the predominance of E. coli meningitis in neonates is not likely due to greater binding and invasion capacities of newborn compared to adult BMECs.
authors.affiliation: Division of Infectious Diseases, Children's Hospital Los Angeles, Los Angeles, California 90027, USA.
authors.forename: M F
authors.initials: MF
authors.lastname: Stins
authors.forename: P V
authors.initials: PV
authors.lastname: Nemani
authors.forename: C
authors.initials: C
authors.lastname: Wass
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000818:Animals; D001422:Bacterial Adhesion; D001921:Brain / Q000098:blood supply* / Q000382:microbiology*; D002648:Child; D004730:Endothelium, Vascular / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D006801:Humans; D016920:Meningitis, Bacterial / Q000209:etiology*; D051381:Rats