Effects of single and repeated stresses on the expression of mRNA for alpha1-adrenoceptors in the rat hypothalamus and midbrain.
We examined the effects of single or repeated stress on the expression of mRNA for alpha1-adrenoceptors in the rat hypothalamus and midbrain using the reverse transcriptase-polymerase chain reaction (RT-PCR). Single stress significantly increased the mRNA level for alpha1-adrenoceptors in the midbrain, but had no effect on mRNA levels in the hypothalamus. Repeated stress significantly decreased mRNA levels for alpha1-adrenoceptors in both regions.
Miyahara
S
S
Department of Psychiatry, Mie University School of Medicine, Tsu, Japan.
Komori
T
T
Fujiwara
R
R
Shizuya
K
K
Yamamoto
M
M
Ohmori
M
M
Okazaki
Y
Y
D000818:Animals; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008636:Mesencephalon / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics*; D012149:Restraint, Physical / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D013997:Time Factors
D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1