Genetic diversity among Escherichia coli O149:K91 strains isolated from pigs with diarrhoea determined by randomly amplified polymorphic DNA analysis.
The genetic relatedness among 72 Escherichia coli strains of serotype O149:K91 isolated from pigs with diarrhoea was investigated by randomly amplified polymorphic DNA (RAPD) analysis. Fimbrial and toxic virulence markers of the isolates were also tested. Amplification with primer 1254 resulted in three different RAPD types whereas primer 1290 generated one RAPD profile only. Based on the RAPD and fimbrial/toxin types the strains were classified into five distinct groups.
Osek
J
J
Department of Microbiology, National Veterinary Research Institute, Partyzantow 57, Pulawy, 24-100, Poland. josek@piwet.pulawy.pl
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D004269:DNA, Bacterial