title: Co-operativity between modules within a C3b-binding site of complement receptor type 1.
abstract: Complement receptor type 1 (CR1) has 30 modules in its extracellular portion. An understanding of structure-function relationships within CR1 is being assembled gradually from studies of overlapping protein fragments. A CR1 fragment corresponding to modules 16 and 17 was expressed recombinantly as a non-glycosylated protein and its stability and unfolding characteristics studied using biophysical techniques. The results were compared with data collected previously on a CR1 fragment encompassing modules 15, 16 and 17 which together constitute a C3b-binding site (Kirkitadze, M.D., Krych, M., Uhrin, D. , Dryden, D.T.F., Smith, B.O., Wang, X., Hauhart, R., Atkinson, J.P. and Barlow, P.N. (1999) Biochemistry 38, 7019-7031). Modules within CR1 were found to co-operate during unfolding. The folding, stability and flexibility of this protein is therefore likely to be a complex function, and not just the sum, of contributions from individual modules.
authors.affiliation: The Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, UK.
authors.forename: M D
authors.initials: MD
authors.lastname: Kirkitadze
authors.forename: D T
authors.initials: DT
authors.lastname: Dryden
authors.forename: S M
authors.initials: SM
authors.lastname: Kelly
authors.forename: N C
authors.initials: NC
authors.lastname: Price
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Krych
authors.forename: J P
authors.initials: JP
authors.lastname: Atkinson
authors.forename: P N
authors.initials: PN
authors.lastname: Barlow
mesh_terms: D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D003179:Complement C3b / Q000737:chemistry / Q000378:metabolism*; D019791:Guanidine / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D010843:Pichia; D011487:Protein Conformation; D017510:Protein Folding; D011951:Receptors, Complement / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D011951:Receptors, Complement; D003179:Complement C3b; D019791:Guanidine