[Polypeptides from influenza virus, obtained in the presence of high concentrations of rimantadine].
FPV/Rostock obtained in the presence of remantadine (25 micrograms/ml) is studied. This variant of the virus is characterized by a decreased amount of M1-protein. Its buoyant density in urograffin density gradient is 1.20 gamma/cm3 vs. 1.18 and 1.175 g/cm3 for initial FPV and remantadine-resistant FPV, respectively. Purification and concentration decreases the hemagglutinating activity of the virus 5-10 times more intensely than that of initial and remantadine-resistant FPV. On the other hand, the production of virus-specific proteins and intracellular distribution of hemagglutinin in this virus variant were not changed. The changes associated with a decrease of hemagglutinin and M1-protein content in virions are believed to manifest at a later stage preceding virion release from the cell.
Boreko
E I
EI
Pavlova
N I
NI
Votiakov
V I
VI
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D009975:Orthomyxoviridae / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D012299:Rimantadine / Q000494:pharmacology*; D014763:Viral Matrix Proteins / Q000737:chemistry
D000998:Antiviral Agents; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; D010455:Peptides; D014763:Viral Matrix Proteins; D012299:Rimantadine