Cloning and sequencing of rpoH and identification of ftsE-ftsX in Pseudomonas putida PpG1.
The rpoH gene encoding the heat-shock sigma factor of Pseudomonas putida was cloned by using its ability to complement the temperature-sensitive growth of the Escherichia coli rpoH mutant. The cloned DNA contained an open reading frame for a 284 amino acid sequence exhibiting high homology to the sigmaH proteins of P. aeruginosa and E. coli. Moreover, homologs to the cell division genes ftsX and ftsE were found immediately upstream of the rpoH gene.
Aramaki
H
H
Department of Molecular Biology, Daiichi College of Pharmaceutical Sciences, Fukuoka, Fukuoka, Japan. haramaki-ind@umin.ac.jp
Sagara
Y
Y
Fujita
M
M
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016958:Pseudomonas putida / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D029968:Escherichia coli Proteins; C491157:FtsX protein, E coli; C102286:FtsX protein, bacteria; D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; C075980:FtsE protein, E coli; D019005:Cystic Fibrosis Transmembrane Conductance Regulator