[A new scheme for the isolation and purification of human alpha-fetoprotein].
A new efficient scheme for the isolation and purification of human alpha-fetoprotein from human cord serum was proposed. Sequential chromatography on four columns (Cibacron Blue Sepharose, two metal chelate, and one reversed-phase) helped rapidly prepare alpha-fetoprotein samples of high purity (no less than 98%; purification factor approximately 10(3)) in high yields (approximately 85%).
Tomashevskiĭ
A Iu
AIu
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russia. tom@ibpm.serpukhov.su
Uverskiĭ
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D002845:Chromatography / Q000379:methods*; D006801:Humans; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D000509:alpha-Fetoproteins