A simple method to identify cysteine residues by isotopic labeling and ion trap mass spectrometry.
A simple method was developed to facilitate the identification of cysteine-containing peptides based on S-carboxymethylation of cysteine residues with isotopically enriched bromoacetic acid. An ion trap mass spectrometer with high-resolution scan functionality allows identification of the unique isotopic distribution patterns specific to the modified peptides.
Adamczyk
M
M
Department of Chemistry (9NM), Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Gebler
J C
JC
Wu
J
J
D000818:Animals; D003545:Cysteine / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000737:chemistry
D010455:Peptides; D003545:Cysteine