Isolation and analysis of amplified cDNA fragments during detection of unknown polymorphisms with temperature modulated heteroduplex chromatography.
A procedure for isolation of polymerase chain reaction (PCR) fragments from an automated system for DNA fragment analysis is described. The technique is used for screening of unknown mutations or polymorphisms. Fragments are isolated during the analysis from the system by an automated unit for high-throughput projects or manually when working with a few samples.
Kuklin
A
A
Transgenomic, Inc., San Jose, CA 95131, USA.
Munson
K
K
Taylor
P
P
Gjerde
D
D
D002845:Chromatography / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D013696:Temperature
D018076:DNA, Complementary