Use of capillary electrophoresis with UV detection as a screening method to determine histamine in fish samples.
Histamine levels in fish, extracted with methanol, were determined by capillary electrophoresis (CE) using phosphate buffer pH 2.5 and U.V. detection at 210 nm. Histamine was well separated from the other co-extracted components under the given CE condition without any cleanup of the methanol extract. The average recovery of spiked histamine in various types of fish samples was 96%. Using the same methanol extracts from various fish samples, we then compared histamine concentration obtained by CE and fluorometric methods.
Liao
W
W
California Department of Health Services, Division of Food, Drug and Radiation Safety, Los Angeles 90015, USA.
Paek
H
H
Mabuni
C
C
Angold
S
S
Soliman
M
M
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005399:Fishes; D006632:Histamine / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D006632:Histamine