Single-copy transgenic wheat generated through the resolution of complex integration patterns.
Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.
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Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, Albany, CA 94710, USA.
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D002678:Chimera; D007446:Chromosome Inversion; D018628:Gene Dosage; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics*