Molecular separation of Oesophagostomum stephanostomum and Oesophagostomum bifurcum (Nematoda: Strongyloidea) from non-human primates.
The ITS-2 sequences for adult specimens of Oesophagostomum stephanostomum from the common chimpanzee and Oesophagostomum bifurcum from the Mona monkey were determined. For both species, the length and GC content of the ITS-2 sequences were 216 bp and 43%, respectively. While there was no unequivocal sequence difference among individual worms representing each of the two species, five (2.3%) interspecific nucleotide differences were detected. These differences were associated with the presence of unique restriction sites in the ITS-2 sequence of 0. stephanostomum for multiple endonucleases of diagnostic value for the differentiation of the two taxa by restriction analysis. Pairwise comparisons of the ITS-2 sequences of O. stephanostomum and O. bifurcum with published ITS-2 sequences for five different congeners indicated that these species from the subgenus Conoweberia are closely related, in accordance with previous morphological studies.
Gasser
R B
RB
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia. r.gasser@vet.unimelb.edu.au
Woods
W G
WG
Huffman
M A
MA
Blotkamp
J
J
Polderman
A M
AM
D000818:Animals; D018420:Ape Diseases / Q000469:parasitology*; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D000882:Haplorhini; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000469:parasitology*; D009814:Oesophagostomiasis / Q000469:parasitology / Q000662:veterinary*; D009815:Oesophagostomum / Q000145:classification* / Q000235:genetics; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018509:DNA, Helminth