A generic protein purification method for protein complex characterization and proteome exploration.
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
Rigaut
G
G
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Shevchenko
A
A
Rutz
B
B
Wilm
M
M
Mann
M
M
Séraphin
B
B
D000345:Affinity Labels; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D008969:Molecular Sequence Data; D011506:Proteins / Q000302:isolation & purification*; D020543:Proteome / Q000737:chemistry*
D000345:Affinity Labels; D011506:Proteins; D020543:Proteome