A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit.
We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants. Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-gammaS, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61beta, a subunit of the heterotrimeric protein conducting channel SEC61p. Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61beta in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61beta subunit.
Ingley
E
E
Laboratory for Cancer Medicine, Department of Biochemistry, The University of Western Australia and Royal Perth Hospital, Perth, W.A., Australia.
Williams
J H
JH
Walker
C E
CE
Tsai
S
S
Colley
S
S
Sayer
M S
MS
Tilbrook
P A
PA
Sarna
M
M
Beaumont
J G
JG
Klinken
S P
SP
D020727:ADP-Ribosylation Factors / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis; D000069816:SEC Translocation Channels; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; D012333:RNA, Messenger; D000069816:SEC Translocation Channels; D020727:ADP-Ribosylation Factors; C122027:Arl6 protein, mouse