No relationship between proliferative activity and the MACIS prognostic scoring system in papillary thyroid carcinoma.
BACKGROUND
The MACIS score uses metastasis, age, completeness of resection, local invasion, and tumor size to stratify patients with papillary thyroid carcinoma (PTC) into four groups with different survival.


METHODS
Immunostaining for proliferating cell nuclear antigen (PCNA) was done in 43 cases of PTC. Relationships between proliferative index (percentage of cells that were PCNA positive) and the MACIS parameters were examined. Double staining for PCNA and for silver-stained nucleolar organizer regions (AgNORs, indicating proliferation) was performed in 10 cases.


RESULTS
PCNA was detected only in tumor cells. The proliferative index was low (mean, 14.2%; median, 13.0%), did not differ between MACIS groups (p = 0.56), and showed no association with individual MACIS parameters. PCNA immunostaining correlated with AgNOR staining. The mean AgNOR count was 2.28 in PCNA-positive cells and 1.85 in PCNA-negative cells (p </= 0.002).


CONCLUSIONS
PCNA immunostaining indicates proliferative activity in PTC. It is independent of the MACIS score and its parameters.
Moreira Leite
K R
KR
Laboratory of Molecular and Surgical Pathology, Sirio Libanes Hospital, Rua Adma Jafet, 91, 01308-050, Sao Paulo, Brazil.
de Araujo
V C
VC
Rezende Meirelles
M I
MI
Lopes Costa
A D
AD
Camara-Lopes
L H
LH
D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D002291:Carcinoma, Papillary / Q000175:diagnosis / Q000473:pathology* / Q000556:secondary / Q000601:surgery; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009697:Nucleolus Organizer Region / Q000473:pathology*; D011237:Predictive Value of Tests; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000473:pathology* / Q000601:surgery
D018809:Proliferating Cell Nuclear Antigen