Selection of human anti-HAV McAb from a phage antibody library.
The phage displaying antibody fragments were subjected to three rounds of panning with hepatitis A virus (HAV) antigen in solid phase. The eluted phage was enriched nearly 100 fold, and the percentage of recombinant clones increased from 25% to 100% after three rounds of panning. The HAV antigen-specific monoclonal antibody (McAb) was screened by sandwich ELISA, and the specificity of these antibodies was further confirmed by competitive inhibition ELISA.
Wan
Z
Z
Department of Microbiology, Third Military Medical University, Chongqing, China.
Wang
H
H
Jiang
S
S
D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D019151:Peptide Library; D015183:Restriction Mapping
D000911:Antibodies, Monoclonal; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D019151:Peptide Library