title: Heat shock cognate 70 mutations in sporadic breast carcinoma.
abstract: Heat Shock Cognate 70 (HSC70) is a constitutively expressed molecular chaperone, functions of which regulate the structure, subcellular localization, and turnover of cell proteins. It is also a component of the centrosome facilitating rearrangements during mitotic/meiotic spindle formation and cytoplasmic microtubule organization. We localized HSC70 to 11q23.3, a region deleted in 40% of sporadic breast and other cancers. Sequencing demonstrated mutation in the NH2-terminal ATPase domain of HSC70 in 2 of 15 sporadic breast carcinomas examined. In both cases, mutation was coincident with allelic imbalance, suggesting that HSC70 is a target of somatic mutation and deletion in a fraction of breast carcinomas.
authors.affiliation: University of Oxford, Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, United Kingdom. Christopher.Bakkenist@stjude.org
authors.forename: C J
authors.initials: CJ
authors.lastname: Bakkenist
authors.forename: J
authors.initials: J
authors.lastname: Koreth
authors.forename: C S
authors.initials: CS
authors.lastname: Williams
authors.forename: N C
authors.initials: NC
authors.lastname: Hunt
authors.forename: J O
authors.initials: JO
authors.lastname: McGee
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011110:Polymorphism, Genetic
chemical_list: D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D000251:Adenosine Triphosphatases