Growth stimulation by serum in Entamoeba histolytica is associated with protein tyrosine dephosphorylation.
Very little protein tyrosine phosphorylation was observed in growing (exponential-phase) Entamoeba histolytica cells by immunoblotting and quantitative immunofluorescence. After 1 h of serum deprivation, two proteins (42 and 38 kDa in SDS-PAGE) were tyrosine phosphorylated and two more proteins (96 and 63 kDa) also showed tyrosine phosphorylation when examined after 4 h of serum deprivation. Intense enhancements of anti-phosphotyrosine immunofluorescence levels were observed during this period of serum withdrawal. Membrane-associated tyrosine kinase activity reached a peak (3.5-fold increase) 1 h after serum deprivation and decreased thereafter reaching a basal level by 2 h of serum deprivation. Interestingly, tyrosine kinase activities remained unaffected by serum stimulation (2-60 min) of serum-deprived cells. Also, during this period of serum stimulation tyrosine phosphorylated proteins of serum-deprived cells were dephosphorylated. Tyrosine phosphatase activities were suppressed during serum deprivation and on serum addition to serum-deprived cells tyrosine phosphatase activities increased significantly. Our data attest that protein tyrosine phosphorylation was associated with growth inhibition of E. histolytica and serum stimulation of E. histolytica produced tyrosine phosphatase activation and protein tyrosine dephosphorylation.
Chaudhuri
S
S
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
Choudhury
N
N
Raha
S
S
D000818:Animals; D001769:Blood; D002417:Cattle; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004748:Entamoeba histolytica / Q000254:growth & development* / Q000378:metabolism; D004789:Enzyme Activation; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015800:Protozoan Proteins / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D015800:Protozoan Proteins; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D017027:Protein Tyrosine Phosphatases