title: Migration of langerhans cells and dermal dendritic cells in skin organ cultures: augmentation by TNF-alpha and IL-1beta.
abstract: Migration from sites of antigen encounter to lymphoid organs is essential to the strong immunogenic function of dendritic cells (DC). In the skin, migration proceeds through dermal lymphatic vessels and is regulated in an incompletely understood way by inflammatory mediators. We studied the effects of tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in mouse skin organ cultures by direct enumeration of migrating DC and by immunohistochemistry. (1) Neutralizing antibodies to TNF-alpha and IL-1beta inhibited migration of DC, also in human skin explants (TNF-alpha). (2) TNF-alpha at low concentrations (50 U/mL) and IL-1beta (50-3000 U/mL) augmented migration to about 150% of spontaneous migration. (3) High concentrations of TNF-alpha (5000 U/mL) inhibited migration by approximately 50%. (4) DC migration from skin explants of TNF-alpha/lymphotoxin-alpha double-deficient mice and TNF-receptor type 1 and 2 double knockout mice was not impaired. (5) TNF-alpha effects were neutralized by anti-IL-1beta, and vice versa. We conclude that in normal animals both TNF-alpha and IL-1beta are required for DC migration to occur. In the complete absence of one cytokine (TNF-alpha), however, backup mechanisms step in.
authors.affiliation: Department of Dermatology, University of Innsbruck, Austria. patrizia.stoitzner@uibk.ac.at
authors.forename: P
authors.initials: P
authors.lastname: Stoitzner
authors.forename: M
authors.initials: M
authors.lastname: Zanella
authors.forename: U
authors.initials: U
authors.lastname: Ortner
authors.forename: M
authors.initials: M
authors.lastname: Lukas
authors.forename: A
authors.initials: A
authors.lastname: Tagwerker
authors.forename: K
authors.initials: K
authors.lastname: Janke
authors.forename: M B
authors.initials: MB
authors.lastname: Lutz
authors.forename: G
authors.initials: G
authors.lastname: Schuler
authors.forename: B
authors.initials: B
authors.lastname: Echtenacher
authors.forename: B
authors.initials: B
authors.lastname: Ryffel
authors.forename: F
authors.initials: F
authors.lastname: Koch
authors.forename: N
authors.initials: N
authors.lastname: Romani
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000737:chemistry; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007801:Langerhans Cells / Q000166:cytology / Q000187:drug effects*; D008233:Lymphotoxin-alpha / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009924:Organ Culture Techniques; D018124:Receptors, Tumor Necrosis Factor / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D012867:Skin / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D008233:Lymphotoxin-alpha; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha