Structure, binding and neutralization of VHSV with synthetic peptides.
The phosphatidylserine binding region p2 of VHSV was characterized and was shown to be involved with fusion. Synthetic peptides corresponding to this region interact with phospholipids by penetrating into the membrane and changing to a beta sheet configuration. Computer modeling of this region shows the possible ways by which the interaction with the membranes can succeed. Inhibitory peptides are presently being sought by studying possible interactions within heptad repeats located in other regions of the G protein of VHSV. The heptad repeat region that includes the phosphatidylserine binding domain p2 has been cloned and preliminary experiments show that under certain conditions, peptides from this region can inhibit VHSV infectivity.
Estepa
A
A
Departmento Bioquimica y Biologia Molecular, Universidad Miguel Hernandez, Elche, Alicante, Spain.
Fernandez-Alonso
M
M
Coll
J M
JM
D000818:Animals; D019921:Biotinylation; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D012209:Rhabdoviridae / Q000737:chemistry* / Q000378:metabolism / Q000472:pathogenicity; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000378:metabolism
C096089:G protein, viral hemorrhagic septicemia virus; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins