title: Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species.
abstract: A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
authors.affiliation: Universidad Complutense, Facultad de Veterinaria, Departmento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Céspedes
authors.forename: T
authors.initials: T
authors.lastname: García
authors.forename: E
authors.initials: E
authors.lastname: Carrera
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005412:Flatfishes / Q000145:classification* / Q000235:genetics; D008928:Mitochondria / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial