title: The effects of several nucleotides on the molecular state and catalytic activity of Thiobacillus novellus cytochrome c oxidase. ATP affects the oxidase uniquely.
abstract: The catalytic activity and molecular aspects of Thiobacillus novellus cytpchrome c oxidase were affected by ATP. The steady-state kinetics in the oxidation of ferrocytochrome c by the oxidase varied with the presence or absence of ATP; the [S]-v curve of the reaction was sigmoid in the absence of ATP whereas it was a Michaelis-Menten-type hyperbola in the presence of 700 microM ATP. The oxidase was a dimer of the minimal structural subunit consisting of one molecule each of two subunits in the presence of Tween 20 and in the absence of ATP. The dimer dissociated into monomers in the presence of 700 microM ATP. The trough at 452 nm seen in the second derivative absorption spectrum of the CO compound of the oxidase in the absence of ATP, a characteristic of the cytochrome a component of cytochrome aa3, dissappeared in the presence of 700 microM ATP. However, ADP, AMP, GTP, CTP and UTP had little affect on both the [S]-v curve and the molecular mass of the oxidase when used in place of ATP.
authors.affiliation: Department of Industrial Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shoji
authors.forename: M
authors.initials: M
authors.lastname: Tanigawa
authors.forename: K
authors.initials: K
authors.lastname: Hori
authors.forename: Y
authors.initials: Y
authors.lastname: Tomozawa
authors.forename: T
authors.initials: T
authors.lastname: Yamanaka
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D019281:Dimerization; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009711:Nucleotides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D013053:Spectrophotometry; D013855:Thiobacillus / Q000201:enzymology*
chemical_list: D003574:Cytochrome c Group; D009711:Nucleotides; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV