Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death.
Challenging tomato or tobacco varieties with ethylene-inducing xylanase (EIX) from the fungus Trichoderma viride causes rapid induction of plant defence responses leading to programmed cell death. Using the yeast two-hybrid system, we isolated a novel protein, tomato small ubiquitin-related modifier protein (T-SUMO), which specifically interacts with EIX. T-SUMO, a cytoplasmic protein, is a member of the ubiquitin-like protein family. It shows homology to human protein sentrin/SUMO1, which suppresses tumour necrosis factor-induced cell death. Transgenic plants that express T-SUMO in the sense orientation suppress EIX induction of ethylene biosynthesis and cell death, while in the antisense orientation they enhance EIX-induced ethylene biosynthesis. These results indicate that T-SUMO is involved in mediating the signal generated by EIX that leads to induction of plant defence responses.
Hanania
U
U
Department of Plant Sciences, Tel Aviv University, Israel.
Furman-Matarasso
N
N
Ron
M
M
Avni
A
A
D000595:Amino Acid Sequence; D016923:Cell Death; D002874:Chromosome Mapping; D005030:Ethylenes / Q000096:biosynthesis*; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D014452:Ubiquitins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000378:metabolism*
D005030:Ethylenes; C403233:SUMO protein, plant; D014452:Ubiquitins; C036216:ethylene; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase