Following the development of a CD4 T cell response in vivo: from activation to memory formation.
The in vivo differentiation of CD4 T cells from naive to memory cells was followed after their adoptive transfer together with syngeneic dendritic cells into MHC mismatched adoptive hosts lacking lymphocytes and NK cells. Functional and molecular changes were measured as the antigenic stimulus, provided by the cotransferred dendritic cells, disappeared. Memory cells as opposed to effector cells show an inversion in the relative expression of Bcl-2 family members in favor of antiapoptotic molecules, and compared with naive cells they have an increased ratio of bcl-xL to bcl-2. They differ qualitatively from naive T cells, suggesting that accelerated CD4 memory responses can occur without the need for increased frequencies of specific T cells.
Garcia
S
S
Division of Molecular Immunology, The National Institute for Medical Research, Mill Hill, London, United Kingdom.
DiSanto
J
J
Stockinger
B
B
D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000502:physiology; D018398:Homeodomain Proteins / Q000502:physiology; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; D018398:Homeodomain Proteins; D007376:Interleukin-2; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; C064658:RAG-1 protein; D007371:Interferon-gamma