Enhanced expression of Tie2, its ligand angiopoietin-1, vascular endothelial growth factor, and CD31 in human non-small cell lung carcinomas.
Expression of angiogenesis-associated genes was compared in 32 primary non-small cell lung carcinoma samples (14 adenocarcinomas, 17 squamous cell carcinomas, and 1 large cell carcinoma) and paired adjacent noncancerous lung tissues using a multiprobe RNase protection assay. Levels of Tie2, angiopoietin (Ang)-1, vascular endothelial growth factor (VEGF), and CD31 mRNAs were higher in cancers than in adjacent noncancerous tissues, in contrast to the fms-like tyrosine kinase (Flt)-1, Flt-4, Tie1, thrombin receptor, endoglin, and VEGF-C, for which no differences were evident. Overexpression did not seem to differ with histological type and pathological stage. Significant positive correlations were found between mRNA expression of Ang-1 and those of Tie2 and CD31, and that of VEGF and those of Flt-1 and CD31. These findings suggest that Ang-1 and VEGF are important angiogenic factors in human non-small cell lung carcinomas.
Takahama
M
M
Department of Oncological Pathology, Cancer Center, Nara Medical University, Kashihara, Japan.
Tsutsumi
M
M
Tsujiuchi
T
T
Nezu
K
K
Kushibe
K
K
Taniguchi
S
S
Kotake
Y
Y
Konishi
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D042683:Angiopoietin-1; D015415:Biomarkers; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008024:Ligands; D008175:Lung Neoplasms / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis*; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D042787:Receptor, TIE-2; D012260:Ribonucleases / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
C467630:ANGPT1 protein, human; D042683:Angiopoietin-1; D015415:Biomarkers; D016228:Endothelial Growth Factors; D008024:Ligands; D008222:Lymphokines; D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2; D012260:Ribonucleases