title: Early suppression of lymphoproliferative response in dogs with natural infection by Leishmania infantum.
abstract: Dogs are the domestic reservoirs of zoonotic visceral leishmaniasis caused by Leishmania infantum. Early detection of canine infections evolving to clinically patent disease may be important to leishmaniasis control. In this study we firstly investigated the peripheral blood mononuclear cell (PBMC) response to leishmanial antigens and to polyclonal activators concanavalin A, phytohemagglutinin and pokeweed mitogen, of mixed-breed dogs with natural L. infantum infection, either in presymptomatic or in patent disease condition, compared to healthy animals. Leishmania antigens did not induce a clear proliferative response in any of the animals examined. Furthermore, mitogen-induced lymphocyte proliferation was found strongly reduced not only in symptomatic, but also in presymptomatic dogs suggesting that the cell-mediated immunity is suppressed in progressive canine leishmaniasis. To test this finding, naive Beagle dogs were exposed to natural L. infantum infection in a highly endemic area of southern Italy. Two to 10 months after exposure all dogs were found to be infected by Leishmania, and on month 2 of exposure they all showed a significant reduction in PBMC activation by mitogens. Our results indicate that suppression of the lymphoproliferative response is a common occurrence in dogs already at the beginning of an established leishmanial infection.
authors.affiliation: Istituto di Clinica Medica Veterinaria, Università Federico II, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: De Luna
authors.forename: M L
authors.initials: ML
authors.lastname: Vuotto
authors.forename: M T
authors.initials: MT
authors.lastname: Ielpo
authors.forename: R
authors.initials: R
authors.lastname: Ambrosio
authors.forename: D
authors.initials: D
authors.lastname: Piantedosi
authors.forename: V
authors.initials: V
authors.lastname: Moscatiello
authors.forename: P
authors.initials: P
authors.lastname: Ciaramella
authors.forename: A
authors.initials: A
authors.lastname: Scalone
authors.forename: L
authors.initials: L
authors.lastname: Gradoni
authors.forename: D
authors.initials: D
authors.lastname: Mancino
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology / Q000494:pharmacology; D003208:Concanavalin A / Q000276:immunology / Q000494:pharmacology; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000662:veterinary*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D010835:Phytohemagglutinins / Q000276:immunology / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000276:immunology / Q000494:pharmacology
chemical_list: D000953:Antigens, Protozoan; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A