New world, but not Old World, monkeys carry several genes encoding beta-microseminoprotein.
It was shown by Southern hybridization that cotton-top tamarin and common marmoset, New World monkeys, carry three or more genes encoding beta-microseminoprotein, also known as PSP94. In contrast, the genomes of Old World monkeys, as represented by rhesus macaque and sacred baboon, contain a single gene. Clones containing three different genes encoding beta-microseminoprotein were isolated from a cotton-top tamarin genomic library. They carry two complete genes of four exons and a third gene lacking the first exon. The structure suggests that the three genes are functionally active and give rise to transcripts that are approximately 86% similar in sequence. By sequencing one gene in full, it was shown that the introns carry an excess of interspersed repeats, on average 29% of the introns consist of Alu repeats. A phylogenetic analysis demonstrated that the genes probably arose in New World monkeys after the separation from Old World primates.
Mäkinen
M
M
Department of Clinical Chemistry, Lund University, University Hospital, Malmö, Sweden.
Valtonen-André
C
C
Lundwall
A
A
D020087:Alu Elements; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002427:Cebidae / Q000235:genetics*; D002520:Cercopithecidae / Q000235:genetics*; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D010802:Phylogeny; D029584:Prostatic Secretory Proteins; D015183:Restriction Mapping; D016415:Sequence Alignment
D010455:Peptides; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein