title: Biochemical and structural studies with neutralizing antibodies raised against foot-and-mouth disease virus.
abstract: The function of a loop exposed on the aphthovirus capsid (the G-H loop of protein VP1) has been explored by combining genetic and structural studies with viral mutants. The loop displays a dual function of receptor recognition and interaction with neutralizing antibodies. Remarkably, some amino acid residues play a critical role in both such disparate functions. Therefore residues subjected to antibody pressure for variation may nevertheless maintain a role in receptor recognition for which invariance is a requirement. Evolution of FMDV in cell culture may relax the requirements at this site and allow further increase of antigenic diversification. Essential residues at one stage of virus evolution may become dispensable at another not very distant point in the evolutionary landscape. Implications for FMDV evolution and vaccine design are discussed.
authors.affiliation: Centro de Biologá Molecular Servero Ochoa, Universidad Autónoma de Madrid, Cantoblanco, Spain. edomingo@cbm.uam.es
authors.forename: E
authors.initials: E
authors.lastname: Domingo
authors.forename: N
authors.initials: N
authors.lastname: Verdaguer
authors.forename: W F
authors.initials: WF
authors.lastname: Ochoa
authors.forename: C M
authors.initials: CM
authors.lastname: Ruiz-Jarabo
authors.forename: N
authors.initials: N
authors.lastname: Sevilla
authors.forename: E
authors.initials: E
authors.lastname: Baranowski
authors.forename: M G
authors.initials: MG
authors.lastname: Mateu
authors.forename: I
authors.initials: I
authors.lastname: Fita
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D005537:Aphthovirus / Q000737:chemistry* / Q000276:immunology*; D002213:Capsid / Q000737:chemistry* / Q000276:immunology; D036022:Capsid Proteins; D002478:Cells, Cultured; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D000939:Epitopes; D006801:Humans; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D011991:Receptors, Virus; C442501:VP1 protein, Foot-and-mouth disease virus