title: Genetic mapping and functional analysis of a nodulation-defective mutant (sym19) of pea (Pisum sativum L.).
abstract: The pea mutant line P55 is defective in root nodule formation, and this phenotype is controlled by a single recessive gene. Complementation analysis revealed that the mutation in P55 is allelic to sym19, which has previously been mapped to linkage group I. Detailed mapping revealed that the sym19 and ENOD40 loci are separated by 2.7 cM. We identified four recombination events, demonstrating that the nodulation defect caused by mutation of the sym19 locus cannot be due to mutation of ENOD40. RT-PCR experiments showed that P55 expresses ENOD12A, but there was little or no increase in the level of its transcript in response to Nod factor or infection with Rhizobium. To investigate this expression pattern further, transgenic peas carrying a pENOD12A-GUS reporter construct were made. One transgenic line was crossed with line P55, to generate F2 progeny homozygous for sym19 and carrying pENOD12A-GUS. In both WT and sym19 mutant lines, ENOD12A-GUS expression was induced at sites of lateral root emergence in uninoculated plants. In Nod+ plants pENOD12A-GUS was induced in response to Rhizobium leguminosarumn bv. viciae, but no such induction was seen in the Nod- (sym19) mutants.
authors.affiliation: John Innes Centre, Norwich Research Park, Colney, UK.
authors.forename: A
authors.initials: A
authors.lastname: Schneider
authors.forename: S A
authors.initials: SA
authors.lastname: Walker
authors.forename: S
authors.initials: S
authors.lastname: Poyser
authors.forename: M
authors.initials: M
authors.lastname: Sagan
authors.forename: T H
authors.initials: TH
authors.lastname: Ellis
authors.forename: J A
authors.initials: JA
authors.lastname: Downie
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D015870:Gene Expression; D017343:Genes, Plant; D017930:Genes, Reporter; D008040:Genetic Linkage; D005966:Glucuronidase / Q000235:genetics; D009154:Mutation; D018532:Pisum sativum / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification; D018517:Plant Roots / Q000382:microbiology*; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016961:Rhizobium leguminosarum; D013559:Symbiosis / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: C062870:ENOD12 protein, Pisum sativum; D010940:Plant Proteins; D005966:Glucuronidase