[Association of MAP kinases with the endoplasmic reticulum in NIH3T3 (HER14) and A431 cells].
Intracellular distribution of p42/p44 MAP-kinases in HER14 and A431 cell lines was investigated. Using subcellular fractionation and immunofluorescence approaches we have shown that in quiescent cells of both types MAP-kinases are associated with endoplasmic reticulum. Moreover, ER-localized MAP-kinases were shown to exist only in a nonphosphorylated form. In HER14 cells the epidermal growth factor (EGF) elevates the level of the ER-associated MAP-kinases. In contrast, exposure of A431 cells to EGF leads to a significant decrease in the observed association. The physiological role of this association is discussed.
Arnautov
A M
AM
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Arnautova
I P
IP
Zheleznova
N N
NN
Kornilova
E S
ES
Nikol'skiĭ
N N
NN
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D051379:Mice; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor; D017871:Calcium-Calmodulin-Dependent Protein Kinases