title: Cell proliferation activity and kinetic profile in the prognosis and therapeutic management of carcinoma of the pharynx and larynx.
abstract: Prognosis and management of carcinoma of the pharynx and larynx is now based on the morphologic analysis of the tumor spreading, differentiation grading, and type of microscopic invasion. The DNA ploidy status and the cell proliferation activity analyzed by flow cytometry give us complementary information about the prognosis and the management and support of the patients. We performed a study of 91 cases of carcinoma of the larynx and pharynx by means of flow cytometry. Forty-three patients were treated by surgery alone, and 48 patients also received radiotherapy. Fifty-five were aneuploid (60%); this percentage increased to 74% in the pharynx area and fell to 47% at the larynx level. The aneuploid tumors showed worse behavior in the patients treated by surgery alone compared with those who also received radiotherapy. The S-phase fraction was high in aneuploid tumors, in positive lymph nodes, and in advanced stages. The S-phase fraction was higher in poorly differentiated tumors. In patients treated by surgery alone, we noticed that by combining both cytometric variables two different kinetic profiles could be defined related to the patients' behavior. The diploid tumors with a low S phase had the greatest rates of survival, whereas diploid tumors with a high rate of S phase and aneuploids had a lower rate.
authors.affiliation: Department of Pathology, University of Oviedo-Hospital Central de Asturias, Oviedo, Spain.
authors.forename: E N
authors.initials: EN
authors.lastname: Myers
authors.forename: A
authors.initials: A
authors.lastname: Sampedro
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.forename: J a
authors.initials: Ja
authors.lastname: Martínez
authors.forename: C
authors.initials: C
authors.lastname: Suárez
authors.forename: M
authors.initials: M
authors.lastname: Alonso-Guervós
authors.forename: J R
authors.initials: JR
authors.lastname: de los Toyos
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000401:mortality / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D002455:Cell Division / Q000502:physiology*; D003131:Combined Modality Therapy; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000401:mortality / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010610:Pharyngeal Neoplasms / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D011003:Ploidies; D011379:Prognosis; D011446:Prospective Studies; D018714:Radiotherapy, Adjuvant; D016196:S Phase / Q000502:physiology; D015996:Survival Rate
chemical_list: D004273:DNA, Neoplasm