Cibacron blue F3G-A-attached uniform and macroporous poly(styrene-co-divinylbenzene) particles for specific albumin adsorption.
Cibacron blue F3G-A-carrying uniform macroporous particles were proposed as an alternative sorbent for specific albumin adsorption. These particles were produced by a multistep polymerization procedure. In the first step of production, the uniform polystyrene seed particles were prepared by a dispersion polymerization method. Next. the polystyrene seed particles were first swollen by dibutylphthalate and then by styrene-divinylbenzene mixture in an aqueous emulsion medium. In the last step (i.e. repolymerization), styrene-divinylbenzene mixture was copolymerized within the swollen seed particles in the absence or presence of a stabilizer (e.g. poly(vinyl alcohol)). Although a considerable amount of non-specific BSA adsorption was observed on the surface of the particles produced in the absence of PVA, zero non-specific albumin adsorption could be achieved with the uniform macroporous particles produced in the presence of PVA. The stabilizer on the particle surface was also used as a ligand in the further derivatization of macroporous particles for specific albumin adsorption. Cibacron blue F3G-A was then covalently attached onto the surface of uniform macroporous particles. Specific albumin adsorption capacities up to 93 mg g(-1) could be achieved with the cibacron blue F3G-A-carrying macroporous particles of 6.25 microm in size.
Camli
S T
ST
Chemistry Department, Hacettepe University, Ankara, Turkey.
Senel
S
S
Tuncel
A
A
D000327:Adsorption / Q000187:drug effects; D004396:Coloring Agents / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics*; D014227:Triazines / Q000737:chemistry*
D004396:Coloring Agents; D011137:Polystyrenes; D014227:Triazines; D012710:Serum Albumin, Bovine; C012842:Cibacron Blue F 3GA; C003771:Amberlite XAD-2 resin