title: Regulation of exocytosis via release of Ca(2+) from intracellular stores.
abstract: The release of Ca(2+) from intracellular stores is an important trigger for secretion in many cell types. Depending on the spatial relationship between the intracellular Ca(2+) stores and the site of exocytosis, the Ca(2+) signal can be very local or spread throughout the entire cell. Here, we review how the release of Ca(2+) from inositol trisphospate (IP(3))-sensitive stores contributes differently to the stimulus-secretion coupling in three types of secretory cells (acinar cells of the pancreas, gonadotrophs, and corticotrophs of the anterior pituitary gland). We propose that in both pancreatic acinar cells and pituitary gonadotrophs the IP(3)-sensitive stores may be in close proximity to the sites of exocytosis such that the concentration of Ca(2+) at these sites are transiently much higher than the average cytosolic Ca(2+) concentration. In contrast, the local Ca(2+) gradient is less prominent in pituitary corticotrophs. Finally, some recent technical developments that may contribute significantly to future investigations of local Ca(2+) signals are discussed.
authors.affiliation: Department of Pharmacology and Division of Neuroscience, University of Alberta, Edmonton, Alberta, Canada. Fred.Tse@ualberta.ca
authors.forename: F W
authors.initials: FW
authors.lastname: Tse
authors.forename: A
authors.initials: A
authors.lastname: Tse
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D006065:Gonadotropins, Pituitary / Q000378:metabolism; D006801:Humans; D010179:Pancreas / Q000166:cytology / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000502:physiology
chemical_list: D006065:Gonadotropins, Pituitary; D000324:Adrenocorticotropic Hormone