Developmental expression of dynamin in the chick retinotectal system.
Dynamin I, a GTPase involved in the endocytic cycle of synaptic vesicle membranes, is believed to support axonal outgrowth and/or synaptogenesis. To explore the temporal and spatial patterns of dynamin I distribution in neuronal morphogenesis, we compared the developmental expression of dynamin with the expression of presynaptic membrane proteins such as SV2, synaptotagmin, and syntaxin in the chick primary visual pathway. Western blots of retina and tectum revealed a steady increase of synaptotagmin and syntaxin from embryonic Day 7 (E7) to E11, whereas for the same time frame no detectable increase of dynamin was found. Later stages showed increasing amounts of all tested proteins until the first postnatal week. Immunofluorescence revealed that SV2, synaptotagmin, and syntaxin are present in retinal ganglion cell axons from E4 on. In later stages, the staining pattern in the retina and along the visual pathway paralleled the formation and maturation of axons. In contrast, dynamin is not detectable by immunofluorescence in the developing retina and optic tectum before synapse formation. Our data indicate that, in contrast to the early expression of synaptotagmin, SV2, and syntaxin during axonal growth, dynamin is upregulated after synapse formation, suggesting its function predominantly during and after synaptogenesis but not in axonogenesis.(J Histochem Cytochem 47:1297-1306, 1999)
Bergmann
M
M
Institute of Anatomy and Special Embryology, University Fribourg, Fribourg, Switzerland.
Grabs
D
D
Rager
G
G
D000375:Aging; D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development; D034284:Dynamin I; D034281:Dynamins; D019084:Fluorescent Antibody Technique, Indirect; D020558:GTP Phosphohydrolases / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D050765:Qa-SNARE Proteins; D012160:Retina / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D013477:Superior Colliculi / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D050857:Synaptotagmins; D013997:Time Factors
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050857:Synaptotagmins; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D034281:Dynamins