Ly-49 receptor expression and functional analysis in multiple mouse strains.
We present data on the strain distribution and functional characteristics of the Ly-49 receptors A, C/I, D, and G2 on DX5+ natural killer (NK) cells. We have examined tyrosine phosphorylation of the Ly-49 molecules, regulation of NK cytotoxic functions, and in vivo marrow rejection capability. The flow cytometry results demonstrate a diverse and complex pattern of expression of the Ly-49 receptors in the 11 strains examined. The vast majority of NK cells express Ly-49s, although some NK1.1+ CD3+ cells also express these receptors. The results of our functional analysis indicate that H-2Dd was able to inhibit the function of Ly-49G2+ NK cells, not only in B6 mice, but also by NK cells derived from several haplotypes. The examination of Ly-49 receptor tyrosine phosphorylation, which is a biochemical measure of inhibitory function, was consistently observed in the 11 mouse strains examined. In contrast, analysis of Ly-49D function suggests its expression appears to be more restricted and that H-2Dd is an activating ligand for this receptor. In addition, the in vivo examination of both inhibitory (Ly-49G2) and activating (Ly-49D) receptors demonstrated regulatory roles of these class I binding receptors in marrow transplantation.
Ortaldo
J R
JR
Laboratory of Experimental Immunology, Division of Basic Sciences, NCI-FCRDC, Frederick, Maryland 21702-1201, USA.
Mason
A T
AT
Winkler-Pickett
R
R
Raziuddin
A
A
Murphy
W J
WJ
Mason
L H
LH
D000818:Animals; D000950:Antigens, Ly; D016026:Bone Marrow Transplantation / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D006239:Haplotypes / Q000235:genetics; D064532:Histocompatibility Antigen H-2D; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D037181:Lectins, C-Type; D008024:Ligands; D008099:Liver / Q000166:cytology / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology*; D008819:Mice, Nude; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology; D011499:Protein Processing, Post-Translational; D011828:Radiation Chimera; D055630:Receptors, NK Cell Lectin-Like; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured
D000950:Antigens, Ly; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D037181:Lectins, C-Type; D008024:Ligands; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D055630:Receptors, NK Cell Lectin-Like; D019000:Phosphotyrosine