Short-chain fatty acids inhibit fluid and electrolyte loss induced by cholera toxin in proximal colon of rabbit in vivo.
Short-chain fatty acids (SCFA), acetate, propionate, and butyrate, are produced by bacterial fermentation in the colon and stimulate Na+ and Cl- absorption. We have studied the effects of SCFA on fluid and electrolyte absorption during cholera toxin (CT) -induced colonic secretion in rabbit, using a steady-state perfusion technique with marker (PEG) dilution. Perfusion with SCFA significantly (P < 0.01) reduced colonic water secretion. Butyrate reduced water secretion the most (95%), followed by propionate (90%), and acetate (80%). Butyrate significantly (P < 0.001) reduced secretions of Na+ (95.5%), K+ (75.2%), and Cl-(80.7%) ions but not HCO3- ions. Propionate similarly reduced secretion of HCO3-(45.3%). Acetate significantly (P < 0.001) inhibited Na+ (76.4%) and Cl- (75.7%) secretion, but the inhibitions of K+ (23.6%) and HCO3 (28.8%) were not significantly different from the controls. We conclude that SCFA reduce CT-induced water and electrolyte secretion in the rabbit proximal colon, and its potential as an antidiarrheal agent should be further evaluated.
Rabbani
G H
GH
Clinical Research and Service Centre, Clinical Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Albert
M J
MJ
Rahman
H
H
Chowdhury
A K
AK
D019342:Acetic Acid / Q000494:pharmacology; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002087:Butyrates / Q000494:pharmacology; D002772:Cholera Toxin / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005260:Female; D017136:Ion Transport / Q000187:drug effects; D008297:Male; D011422:Propionates / Q000494:pharmacology; D011817:Rabbits
D002087:Butyrates; D004573:Electrolytes; D005232:Fatty Acids, Volatile; D011422:Propionates; D002772:Cholera Toxin; C029658:propionic acid; D019342:Acetic Acid