Effect of pinacidil on the contractile response of cyclophosphamide-treated rat vas deferens.
Effect of pinacidil, a K+ channel opener, was studied on contractility of cyclophosphamide-treated rat vas deferens. The mean IC50 value of pinacidil against 1 mmol barium chloride induced rhythmic contractions and 40 mmol potassium chloride induced tonic contractions was significantly (P < 0.01 and P < 0.001, respectively) increased in the cyclophosphamide treated group as compared to the control. The mean EC50 value of norepinephrine (NE) in the presence of pinacidil (10(-6) mol) was significantly (P < 0.001) increased in the cyclophosphamide treated group. These findings indicate that the responsiveness of rat vas deferens smooth muscle to pinacidil is reduced following cyclophosphamide treatment.
Reddy
A G
AG
Division of Pharmacology & Toxicology, Indian Veterinary Research Institute, Izatnagar.
Prakash
V R
VR
Tripathi
H C
HC
Tandan
S K
SK
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism; D003520:Cyclophosphamide / Q000633:toxicity*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D020110:Pinacidil / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D014649:Vas Deferens / Q000187:drug effects* / Q000502:physiology
D018906:Antineoplastic Agents, Alkylating; D015221:Potassium Channels; D020110:Pinacidil; D003520:Cyclophosphamide; D002118:Calcium; D009638:Norepinephrine