title: Analysis of the Corynebacterium glutamicum dapA promoter.
abstract: Deletion and mutational analysis of the promoter P-dapA from Corynebacterium glutamicum was performed to identify regions and particular nucleotides important for its function. An extended -10 region and a stretch of six T's at positions -55 to -50 were found to be the most important elements in the promoter function. The results of mutational analysis of P-dapA are consistent with the conclusions of statistical computer-aided analysis of 44 C. glutamicum promoter sequences.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, CZ-14220 Praha 4, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Vasicová
authors.forename: M
authors.initials: M
authors.lastname: Pátek
authors.forename: J
authors.initials: J
authors.lastname: Nesvera
authors.forename: H
authors.initials: H
authors.lastname: Sahm
authors.forename: B
authors.initials: B
authors.lastname: Eikmanns
mesh_terms: D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000201:enzymology / Q000235:genetics*; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion
chemical_list: D015500:Chloramphenicol O-Acetyltransferase; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase