title: Evaluation in rabbits of the fetal effects of maternal ingestion of Solanum malacoxylon.
abstract: A study was performed to determine the possible toxic effects on the young of does that had been fed during the gestational period on a ration containing Solanum malacoxylon (Sm), a calcinogenic plant that contains a vitamin D3-glycoside conjugate. Experimental animals received a ration containing 0.03% or 0.04% of Sm leaves on days 6 to 30 of gestation. The levels of calcium, phosphorus and alkaline phosphatase in their sera, as well as their feed intake and body weight, were evaluated weekly. The does were euthanized 3 days after parturition and paraffin sections stained with haematoxylin and eosin were prepared from their heart, lungs, kidneys and aorta for histopathological examination. The young from does in the Sm 0.03% group were euthanized 3 days after birth and biochemical and histopathological determinations were performed, as described for the does. The does in both experimental groups showed decreased feed consumption and those in the Sm 0.04% group showed lower body weights throughout their gestation. Animals treated with Sm 0.04% presented a high incidence of abortion and stillbirth. There were biochemical and histopathological alterations in both experimental groups, which were more prominent in the does in the Sm 0.04% group. Litters from does treated with Sm 0.03% showed mineralization of soft tissue and an increase in phosphorus and calcium levels. These findings indicate that the vitamin D3-glycoside passes through the placental barrier to the fetus.
authors.affiliation: Research Centre for Veterinary Toxicology (CEPTOX), Department of Pathology, Faculty of Veterinary Medicine, University of São Paulo, SP, Brazil.
authors.forename: S L
authors.initials: SL
authors.lastname: Górniak
authors.forename: M L
authors.initials: ML
authors.lastname: Dagli
authors.forename: P C
authors.initials: PC
authors.lastname: Maiorka
authors.forename: P C
authors.initials: PC
authors.lastname: Raspantini
authors.forename: M M
authors.initials: MM
authors.lastname: Jericó
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000473:pathology; D001835:Body Weight; D002114:Calcinosis / Q000503:physiopathology / Q000662:veterinary*; D002118:Calcium / Q000097:blood; D004435:Eating; D005260:Female; D006651:Histocytochemistry; D008168:Lung / Q000473:pathology; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D010758:Phosphorus / Q000097:blood; D010947:Plants, Toxic; D011247:Pregnancy; D011817:Rabbits; D019657:Solanaceae / Q000633:toxicity*; D012991:Solanaceous Alkaloids / Q000633:toxicity*
chemical_list: D012991:Solanaceous Alkaloids; D010758:Phosphorus; D000469:Alkaline Phosphatase; D002118:Calcium