title: Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans.
abstract: Candida albicans is an important opportunistic pathogen, whose interaction with cells of the immune system, in particular macrophages (MO), is poorly understood. In order to learn more about the nature of the infectious mechanism, internalisation of Candida albicans was studied in mouse MO by confocal immunofluorescence and electron microscopy in comparison with latex beads of similar size, which were coated with mannosyl-lipoarabinomannan (ManLAM) to target the MO mannose receptor (MR). Uptake of Candida yeasts had characteristics of phagocytosis, required intact actin filaments, and depended on the activity of protein kinase C (PKC). Candida phagosomes rapidly attracted lysosome-associated membrane protein (Lamp)-rich vacuoles, indicative of fusion with late endosomes and lysosomes. Rapid recruitment of late endosomes and lysosomes could be observed regardless of heat-inactivation or serum-opsonisation of Candida, but did not follow binding of the mannosylated-beads to MO, which suggest that this phenotype is not MR-specific. The yeasts developed germ tubes within phagolysosomes, distended their membranes and escaped, destroying the non-activated MO. The filamentous form of Candida could penetrate intact MO even when phagocytosis was blocked, and also attracted Lamp-rich organelles. Inhibition of lysosomal acidification and associated lysosomal fusion reduced germ tube formation of Candida within the phagolysosomes. These data suggest that rapid recruitment of late endocytic/lysosomal compartments by internalizing C. albicans favours survival and virulence of this pathogen.
authors.affiliation: Department of Pediatrics, University School of Medicine, Debrecen, H-4012 Debrecen, POB: 32, Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Káposzta
authors.forename: L
authors.initials: L
authors.lastname: Maródi
authors.forename: M
authors.initials: M
authors.lastname: Hollinshead
authors.forename: S
authors.initials: S
authors.lastname: Gordon
authors.forename: R P
authors.initials: RP
authors.lastname: da Silva
mesh_terms: D000143:Acids / Q000494:pharmacology; D000818:Animals; D002176:Candida albicans / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D002177:Candidiasis / Q000276:immunology*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D011992:Endosomes / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D058851:GPI-Linked Proteins; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000502:physiology* / Q000648:ultrastructure; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008863:Microspheres; D010588:Phagosomes / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D014966:Xanthenes
chemical_list: D000143:Acids; D015816:Cell Adhesion Molecules, Neuronal; D005456:Fluorescent Dyes; D058851:GPI-Linked Proteins; D014966:Xanthenes; C095362:limbic system-associated membrane protein; C034657:Texas red; D016650:Fluorescein-5-isothiocyanate