Co-localization of GABA with nicotinamide adenine dinucleotide phosphate-dependent diaphorase in neurones in the dorsolateral periaqueductal grey matter of the rat.
Co-localization of GABA and nitric oxide synthase (NOS) has been investigated in neurones in the dorsolateral sector of the periaqueductal grey matter (PAG) in rats. GABA-immunoreactive neurones were found throughout the PAG although their density was greatest in the dorsolateral sector. Neurones containing nicotinamide adenine dinucleotide phosphate-dependent diaphorase, used to indicate NOS, were confined to the dorsolateral sector. GABA immunoreactivity was detected in 26.9% of 3009 diaphorase-reactive neurones counted at all rostro-caudal levels of the PAG in material from three rats. This double-labelled population may act as a neuronal gain control system which sets the level of excitability and responsiveness of effector systems in other sectors of the PAG.
Lovick
T A
TA
Department of Physiology, University of Birmingham, UK. t.a.lovick@bham.ac.uk
Paul
N L
NL
D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000201:enzymology; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D010487:Periaqueductal Gray / Q000166:cytology* / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D005680:gamma-Aminobutyric Acid; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D009252:NADPH Dehydrogenase