Effect of staphylococcal enterotoxin B injection on the development of experimental autoimmune encephalomyelitis: influence of cytokine and inducible nitric oxide synthase production.
Possible mechanisms involved in the protective effect of staphylococcal enterotoxin B (SEB) injection on the subsequent development of experimental autoimmune encephalomyelitis (EAE) were investigated. Only partial clonal deletion and anergy of Vbeta8 + T-lymphocytes were documented after myelin basic protein immunization in SEB injected mice. Brain permeability was not influenced. Within the brain or during in vitro rechallenge assays SEB protected mice produced significantly more IL-10, IL-4, TNF-alpha and iNOS. It is suggested that the immune deviating effect of SEB may be involved in its EAE protective effect.
Kuschnaroff
L M
LM
Laboratory for Experimental Transplantation and Rega Institute, University of Leuven, Belgium.
Overbergh
L
L
Sefriouni
H
H
Sobis
H
H
Vandeputte
M
M
Waer
M
M
D000818:Animals; D001812:Blood-Brain Barrier / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D017931:DNA Primers; D018076:DNA, Complementary; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism*; D004768:Enterotoxins / Q000494:pharmacology*; D005070:Evans Blue / Q000493:pharmacokinetics; D005260:Female; D017353:Gene Deletion; D007108:Immune Tolerance; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018664:Interleukin-12 / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D004676:Myelin Basic Protein / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000276:immunology / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013116:Spinal Cord / Q000276:immunology; D013154:Spleen / Q000276:immunology; D018089:Superantigens / Q000494:pharmacology; D018417:Th1 Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D004768:Enterotoxins; D007135:Immunoglobulin Variable Region; D004676:Myelin Basic Protein; D012333:RNA, Messenger; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; C031818:enterotoxin B, staphylococcal; D005070:Evans Blue; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse