title: Abnormal accumulation of G2/M-phase cells from LEC strain rats after X-irradiation at S phase.
abstract: The effect of X-irradiation on the progression of the cell cycle in cell lines from LEC and WKAH rats was investigated by a flow cytometer. When the cells were exposed to 5 Gy of X-rays at S phase, the proportion of S-phase cells in both cell populations decreased with incubation time and that of G2/M-phase cells was approximately 80% at 6 hr post-irradiation. At 12 hr post-irradiation, approximately 45% of the WKAH rat cells appeared in the G1 phase. However, 80-90% of LEC rat cells remained in the G2/M phase and less than 5% in the G1 phase during 6-12 hr post-irradiation. Thus, the LEC rat cells irradiated at S phase remained in the G2/M phase for at least 6 hr longer than did the WKAH rat cells.
authors.affiliation: Department of Veterinary Radiology, Faculty of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Kuzumi
authors.forename: S
authors.initials: S
authors.lastname: Arai
authors.forename: T
authors.initials: T
authors.lastname: Okui
mesh_terms: D000818:Animals; D016195:G2 Phase; D008938:Mitosis; D051381:Rats; D020332:Rats, Inbred LEC; D016196:S Phase / Q000528:radiation effects*; D014916:Whole-Body Irradiation