Inducible nitric oxide synthase inhibitors from Melia azedarach var. japonica.
In bioassay-guided search for inducible nitric oxide synthase (iNOS) inhibitory compounds from higher plants of South Korea, two beta-carboline alkaloids, 4-methoxy-1-vinyl-beta-carboline (1) and 4,8-dimethoxy-l-vinyl-beta-carboline (2) have been isolated from the cortex of Melia azedarach var. japonica. The structures of these compounds were elucidated on the basis of spectroscopic data. Compounds 1 and 2 showed marked inhibitory activity of iNOS on LPS- and interferon-gamma-stimulated RAW 264.7 cells.
Kwon
H C
HC
College of Pharmacy, Sung Kyun Kwan University, Suwon, Korea.
Lee
B G
BG
Kim
S H
SH
Jung
C M
CM
Hong
S Y
SY
Han
J W
JW
Lee
H W
HW
Zee
O P
OP
Lee
K R
KR
D002243:Carbolines / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007723:Korea; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
C121891:4,8-dimethoxy-1-vinylcarboline; C121890:4-methoxy-1-vinylcarboline; D002243:Carbolines; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D010936:Plant Extracts; D007371:Interferon-gamma; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II