title: Gill endocrine cells in the goldfish Carassius carassius var. auratus and their impairment following experimental lead intoxication.
abstract: The presence of endocrine cells in the gills of the goldfish Carassius carassius and the effects of lead intoxication (5 mg l(-1)) on their relative abundance and secretory activity were studied. Endocrine cells synthesizing peptido-like (met and leu-enkephalin and neuropeptide Y) and serotonin-like substances were detected in gill filaments by immunocytochemical procedures. Decreased immunoreactivity for two enkephalins and neuropeptide Y was observed after 48 and 96 h lead exposure. In contrast, increased serotonin immunoreactivity was evident after a one-week treatment. A search for Ca++-binding proteins was also carried out by immunocytochemical methods. Calbindin D-28K and S-100-like molecules were detected in gill epithelium, and their expression appeared enhanced after lead exposure.
authors.affiliation: Department of Animal Biology, Università di Modena and Reggio Emila, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Franchini
authors.forename: B
authors.initials: B
authors.lastname: Rebecchi
authors.forename: A M
authors.initials: AM
authors.lastname: Bolognani Fantin
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002347:Carps; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure; D004745:Enkephalins / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D016166:Free Radical Scavengers / Q000378:metabolism; D005880:Gills / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007854:Lead / Q000633:toxicity*; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D009479:Neuropeptides / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
chemical_list: D002135:Calcium-Binding Proteins; D004745:Enkephalins; D016166:Free Radical Scavengers; D009479:Neuropeptides; D007854:Lead; D012701:Serotonin