Adherence of Fusobacterium necrophorum subspecies necrophorum to different animal cells.
The adherence of Fusobacterium necrophorum subsp. necrophorum to the surfaces of animal cells was studied in order to elucidate the differences between the bacterial appearance in clinical specimens from various animals. The bacterial cells had a strong affinity for murine and rabbit cheek cell surfaces. The bacterium showed a moderate affinity for goat cells, whereas it adhered not so well to canine, feline, human or porcine cells. Treatment of the bacterial cells with haemagglutinin antiserum prior to the binding assay reduced the degree of attachment to murine and rabbit cells. Scanning electron microscopy revealed that the adherent fusobacteria often penetrated into murine and rabbit cell membranes. These observations indicate that the bacterial attachment contributes to the establishment of the infection in mice and rabbits. It is suggested that the weak binding ability resulted in a low incidence of the bacterium in canine, feline and porcine lesions.
Okada
Y
Y
Laboratory of Animal Health, Tokyo University of Agriculture, Japan.
Kanoe
M
M
Yaguchi
Y
Y
Watanabe
T
T
Ohmi
H
H
Okamoto
K
K
D000328:Adult; D001422:Bacterial Adhesion; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005675:Fusobacterium necrophorum / Q000378:metabolism* / Q000472:pathogenicity; D006388:Hemagglutinins / Q000032:analysis / Q000276:immunology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence
D006388:Hemagglutinins