Hyperactivity caused by a nitric oxide synthase inhibitor is countered by ultra-wideband pulses.
Potential action of ultra-wideband (UWB) electromagnetic field pulses on effects of N(G)-nitro- L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase (NOS), on nociception and locomotor activity was investigated in CF-1 mice. Animals were injected IP with saline or 50 mg/kg L-NAME and exposed for 30 min to no pulses (sham exposure) or UWB pulses with electric field parameters of 102+/-1 kV/m peak amplitude, 0.90+/-0.05 ns duration, and 160+/-5 ps rise time (mean+/-S.D.) at 600/s. Animals were tested for thermal nociceptive responses on a 50 degrees C surface and for spontaneous locomotor activity for 5 min. L-NAME by itself increased mean first-response (paw lift, shake, or lick; jump) and back-paw-lick response latencies and mean locomotor activity. Exposure to UWB pulses reduced the L-NAME-induced increase in back-paw-lick latency by 22%, but this change was not statistically significant. The L-NAME-induced hyperactivity was not present after UWB exposure. Reduction and cancellation of effects of L-NAME suggest activation of opposing mechanism(s) by the UWB pulses, possibly including increase of nitric oxide production by NOS. The action, or actions, of UWB pulses appears to be more effective on locomotor activity than on thermal nociception in CF-1 mice.
Seaman
R L
RL
McKesson BioServices and Microwave Bioeffects Branch, U.S. Army Medical Research Detachment, Brooks AFB, Texas 78235-5460, USA. seaman@aloer.brooks.af.mil
Belt
M L
ML
Doyle
J M
JM
Mathur
S P
SP
D000704:Analysis of Variance; D000818:Animals; D004574:Electromagnetic Fields; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000009:adverse effects*; D006948:Hyperkinesis / Q000139:chemically induced* / Q000517:prevention & control; D007274:Injections, Intraperitoneal; D008124:Locomotion / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage / Q000009:adverse effects*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000528:radiation effects; D009619:Nociceptors / Q000187:drug effects / Q000528:radiation effects; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D017288:Pain Threshold / Q000187:drug effects / Q000528:radiation effects; D011930:Reaction Time / Q000187:drug effects / Q000528:radiation effects; D012965:Sodium Chloride; D013997:Time Factors
D004791:Enzyme Inhibitors; D012965:Sodium Chloride; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester