Factors influencing leucine catabolism by a strain of Staphylococcus carnosus.
The metabolism of leucine by resting cells of Staphylococcus carnosus 833 was studied according to three physicochemical factors: preculture condition (defined medium; complex medium), nitrate concentration (0% and 0.03%) and stirring condition (static or shaking). A factorial design was set up to test the effects of these factors, each at two levels. The results showed that resting cells of S. carnosus 833 produced 3-methyl butanal, 3-methyl butanol and 3-methyl butanoic acid from leucine. Whatever the incubation conditions, there was greater quantity of 3-methyl butanoic acid than 3-methyl butanal and 3-methyl butanol. The preculture and incubation conditions influenced the level of production of the 3 metabolites. The highest overall production of the 3 metabolites was observed when cells were incubated without nitrate in the reaction mixture. 3-methylbutanoic acid production was enhanced when S. carnosus 833 was precultivated in complex medium. 3-methylbutanal was only detected when cells were precultivated in defined medium. Stirring condition had no effect on leucine catabolism of S. carnosus 833.
Masson
F
F
Laboratoire de Microbiologie, Station de Recherches sur la Viande, INRA, Saint-Genès Champanelle, France.
Hinrichsen
L
L
Talon
R
R
Montel
M C
MC
D008401:Gas Chromatography-Mass Spectrometry; D007930:Leucine / Q000378:metabolism*; D008956:Models, Chemical; D013210:Staphylococcus / Q000378:metabolism*
D007930:Leucine