Diagnostic and prognostic potential of antibodies against O-acetylated sialic acids in canine visceral leishmaniasis.
Employing bovine submaxillary mucin (BSM) as the coating agent, an enzyme-linked immunosorbent assay (BSM-ELISA) was developed to detect antibodies directed against O-acetylated sialic acids (O-AcSA) in canine visceral leishmaniasis (CVL). Serum samples were collected from 50 dogs previously screened by a parasite-ELISA to detect anti-leishmanial antibodies and designated as seropositive (n = 30) and seronegative (n = 20). The BSM-ELISA detected anti-O-AcSA antibodies in 29 out of 30 seropositive dogs and was negative in 15 out of 20 seronegative dogs; the sensitivity and specificity of the assay being 96.6% and 75%, respectively. Seven dogs from an endemic area in central Israel were longitudinally monitored for 15 months clinically, serologically and cultured for parasite. The levels of antibodies directed against O-AcSA increased with the appearance of clinical symptoms and/or seropositivity, disappeared when the disease was self-limiting as also with chemotherapeutic response and reappeared with relapse. The BSM-ELISA, therefore, represents a valuable tool for assessment of disease progression.
Chatterjee
M
M
Department of Immunobiology, Indian Institute of Chemical Biology, Jadavpur, Calcutta.
Baneth
G
G
Jaffe
C L
CL
Sharma
V
V
Mandal
C
C
D000107:Acetylation; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D002417:Cattle; D004283:Dog Diseases / Q000175:diagnosis* / Q000503:physiopathology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D051379:Mice; D011379:Prognosis; D012794:Sialic Acids / Q000276:immunology*
D000913:Antibodies, Protozoan; D012794:Sialic Acids