title: Purification, crystallization and preliminary crystallographic study of a Kunitz-type trypsin inhibitor from Delonix regia seeds.
abstract: The Kunitz-type trypsin inhibitor from seeds of Flamboyant (Delonix regia) has been purified to homogeneity and plate-like crystals suitable for X-ray analysis have been grown by the hanging-drop method using PEG 6000 as a precipitant. The crystals belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 32.15, b = 69.39, c = 72.54 A. X-ray diffraction data have been collected to 2.95 A resolution. The structure has been solved by molecular replacement using the known structures of trypsin inhibitors from Erythrina caffra seeds (PDB code 1tie) and from soya beans (Glycine max; PDB code 1ba7) as search models.
authors.affiliation: Laboratório Nacional de Luz Síncrotron, Caixa Postal 6192, 13083-970 Campinas, SP, Brazil. igor@lnls.br
authors.forename: I
authors.initials: I
authors.lastname: Polikarpov
authors.forename: A M
authors.initials: AM
authors.lastname: Golubev
authors.forename: L A
authors.initials: LA
authors.lastname: Perles
authors.forename: S C
authors.initials: SC
authors.lastname: Pando
authors.forename: J C
authors.initials: JC
authors.lastname: Novello
authors.forename: S
authors.initials: S
authors.lastname: Marangoni
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D012639:Seeds / Q000737:chemistry*; D014197:Trees; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014360:Trypsin Inhibitor, Kunitz Soybean