title: Cloning, functional characterization, and expression of a gonadotropin receptor cDNA in the ovary and testis of amago salmon (Oncorhynchus rhodurus).
abstract: A gonadotropin receptor was cloned from amago salmon (Oncorhynchus rhodurus) ovarian follicles. This receptor (sGTH-R) belongs to the glycoprotein hormone receptor family with a large extracellular and seven-transmembrane domains. Its sequence homology is highest with mammalian LH receptors. Phylogenetic analysis reveals that sGTH-R is grouped with mammalian and chicken FSH and LH receptors, but not with mammalian TSH receptors. sGTH-R is expressed dominantly in the ovary and testis. Functional characterization examined with transiently transfected mammalian cells revealed increased intracellular cAMP level when exposed to mammalian and fish gonadotropins; the most potent hormone was salmon GTH II. These results indicate that the cloned cDNA encodes a functional amago salmon GTH receptor protein.
authors.affiliation: Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Aichi, 444-8585, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Oba
authors.forename: T
authors.initials: T
authors.lastname: Hirai
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshiura
authors.forename: M
authors.initials: M
authors.lastname: Yoshikuni
authors.forename: H
authors.initials: H
authors.lastname: Kawauchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nagahama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D017683:Oncorhynchus / Q000235:genetics*; D010053:Ovary / Q000737:chemistry*; D011967:Receptors, Gonadotropin / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011974:Receptors, LH / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000737:chemistry*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D011967:Receptors, Gonadotropin; D011974:Receptors, LH; D011994:Recombinant Proteins; D000242:Cyclic AMP