APH-1, a POU homeobox gene expressed in the salt gland of the crustacean Artemia franciscana.
We characterized the first POU-homeoprotein in a crustacean (designated APH-1 for Artemia POU-Homeoprotein, EMBL Y15070). The amino acid sequence of the APH-1 POU-domain is identical, except for two residues, to that of the two class III POU proteins Cf1-a (Drosophila) and POU-M1 (Bombyx mori). Southern blot analysis suggests that crustaceans have only one class III POU gene. RT-PCR and whole-mount in situ hybridization show that APH-1 mRNA is present in larvae specifically in the salt gland, an organ which is involved in osmoregulation, and disappears in the adult.
Chavez
M
M
University of Liège, Laboratoire de Biologie Moléculaire et de Génie Génétique, Tilman, Belgium.
Landry
C
C
Loret
S
S
Muller
M
M
Figueroa
J
J
Peers
B
B
Rentier-Delrue
F
F
Rousseau
G G
GG
Krauskopf
M
M
Martial
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015139:Blotting, Southern; D029742:Caenorhabditis elegans Proteins; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012491:Salt Gland / Q000378:metabolism*; D013997:Time Factors
C405066:APH-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D018398:Homeodomain Proteins; D004262:DNA Restriction Enzymes