title: [Value of fluorescein angiography in control of retinal thermal damage due to diode laser].
abstract: OBJECTIVE
  To evaluate on an ocular ophthalmic model the interest of digitized fluorescein angiography for a control of laser induced thermal damage.
  
  
  METHODS
  After anesthesia, retinal photocoagulation was performed on 6 rabbit eyes with a 810 nm diode laser (p = 100 to 400 mW, phi = 500 microns, 1s) (OcuLight, IRIS Medical Instruments Inc., USA). Fluorescein angiography was then performed with measurements of fluorescence intensity in the area of the laser exposures (CF-60UVi Canon-Europe, The Netherlands; OcuLab, Life Science Resources Ltd, England).
  
  
  RESULTS
  Image analysis shows that the laser lesions stained progressively. For dosages up to 100 +/- 20 mW fluorescence intensity at the center of the laser spot could be modelized with: Icentrer = Imax [(1-e-alpha t)]-Ce beta t with alpha = 0.2 (s-1), beta = 0.46 (s-1); Imax 230 and C = 0.92. For dosages superiors to 200 +/- 20 mW the modelization was: Icenter = Imax [(1-e-alpha t)] with alpha = 0.2 (s-1 and Imax = 230.
  
  
  CONCLUSIONS
  This study demonstrates that quantitative assessment of laser-induced damage to the retina is feasible using fluorescence imaging. The quantification of fluorescence staining in terms of both intensity and time can contribute to a better quantification of laser-induced damage.
authors.affiliation: Centre Ophtalmologique d'Imagerie et Laser, Lambersat. desmettre@lille-inserm.fr
authors.forename: T
authors.initials: T
authors.lastname: Desmettre
authors.forename: J M
authors.initials: JM
authors.lastname: Devoisselle
authors.forename: S
authors.initials: S
authors.lastname: Soulie-Begu
authors.forename: S
authors.initials: S
authors.lastname: Mordon
mesh_terms: D000818:Animals; D001813:Blood-Retinal Barrier; D005451:Fluorescein Angiography; D006358:Hot Temperature; D017075:Laser Coagulation; D007834:Lasers / Q000009:adverse effects*; D008962:Models, Theoretical; D011817:Rabbits; D012160:Retina / Q000293:injuries* / Q000601:surgery*; D013997:Time Factors