title: The pattern of change in salivary immunoglobulins and antibodies to S. mitis and S. oralis in children undergoing bone marrow transplantation: use of an indirect method of assessment.
abstract: The objective of this study was to assess the pattern of change in salivary immunoglobulins and antibodies to S. mitis and S. oralis in 23 children following allogeneic bone marrow transplantation and their matched controls. To overcome the difficulty of obtaining a sufficient quantity of whole saliva from very young, sick children saliva was collected in a 5-ml oral rinse of sterile normal saline. It was not possible to measure the volume of whole saliva in each rinse and the concentration of the salivary immunoglobulins and bacterial antibodies were estimated from 1 ml of oral rinse. Despite these shortcomings a pattern of change in the mean concentrations of total salivary IgA, secretory IgA, antibodies to S. mitis and S. oralis and total IgG at specific event- related times during the transplantation period has been demonstrated. There was a significant increase in the concentration of salivary IgG 7 days post-transplantation, followed by significant decreases in total salivary IgA, secretory IgA and antibodies to S. mitis after recovery of the peripheral neutrophil count above 0.5 x 10(9). The concentrations of total IgA and antibodies to S. oralis was significantly greater in the transplant group 119 days post-transplantation.
authors.affiliation: Department of Oral Medicine, Eastman Dental Institute, University of London, London, UK.
authors.forename: V
authors.initials: V
authors.lastname: Lucas
authors.forename: S
authors.initials: S
authors.lastname: Marchant
authors.forename: S
authors.initials: S
authors.lastname: Challacombe
authors.forename: G
authors.initials: G
authors.lastname: Roberts
authors.forename: D
authors.initials: D
authors.lastname: Beighton
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D016026:Bone Marrow Transplantation; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D017053:Infection Control; D008297:Male; D009055:Mouth / Q000382:microbiology; D015203:Reproducibility of Results; D012463:Saliva / Q000276:immunology*; D013048:Specimen Handling; D013291:Streptococcus / Q000276:immunology*; D019533:Streptococcus oralis / Q000276:immunology*; D007507:Therapeutic Irrigation; D019172:Transplantation Conditioning
chemical_list: D000907:Antibodies, Bacterial; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G