Preparation of specific antisera to 15alpha-hydroxyestrogens.
The synthesis of haptens of 15alpha-hydroxyestrone, 15alpha-hydroxyestradiol, and 15alpha-hydroxyestriol (estetrol) was undertaken, to obtain specific antisera required for enzyme immunoassay. 3-(1-Carboxypropyl) ethers of these 15alpha-hydroxyestrogens were prepared and conjugated with bovine serum albumin and horseradish peroxidase. The specificity of antisera elicited against bovine serum albumin conjugates was checked by the enzyme immunoassay by using horseradish peroxidase-labeled antigen, and proved to be satisfactory in terms of cross-reactivities to related compounds.
Suzuki
E
E
Hatano Research Institute, Food and Drug Safety Center, Hadano, Kanagawa, Japan. e-suzuki@ina-research.co.jp
Nakagomi
M
M
Hashimoto
M
M
Agui
M
M
Iida
S
S
Konno
K
K
Hara
Y
Y
Kurihara
H
H
Matsuki
Y
Y
Imai
K
K
Ono
H
H
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004958:Estradiol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D004970:Estrone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D006894:Hydroxyestrones / Q000138:chemical synthesis* / Q000276:immunology*; D007106:Immune Sera / Q000737:chemistry* / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013388:Succinimides / Q000737:chemistry
C122179:15-hydroxyestrone; D006894:Hydroxyestrones; D007106:Immune Sera; D013388:Succinimides; D012710:Serum Albumin, Bovine; D004970:Estrone; D004958:Estradiol; C011856:15 alpha-hydroxyestradiol; C001426:N-hydroxysuccinimide