Analysis of the cps1 gene provides evidence for a septation checkpoint in Schizosaccharomyces pombe.
The fission yeast gene cps1, which encodes the catalytic subunit of beta-glucan synthase, was isolated in a screen for mutants that show an increase in ploidy at the restrictive temperature. cps1 mutants display defects in both polarity and septation at the permissive temperature, and become swollen and multinucleate at the restrictive temperature. Analysis of the interaction of cps1 with other mutations suggests the existence of a septation checkpoint, which requires the activity of the protein kinase weel for function.
Le Goff
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X
Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), Epalinges.
Woollard
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A
Simanis
V
V
D000199:Actins / Q000302:isolation & purification; D018797:Cell Cycle Proteins; D002455:Cell Division; D002473:Cell Wall / Q000378:metabolism*; D004271:DNA, Fungal / Q000302:isolation & purification; D005434:Flow Cytometry; D005800:Genes, Fungal; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins; D011003:Ploidies; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D013696:Temperature; D014404:Tubulin / Q000302:isolation & purification
D000199:Actins; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D008565:Membrane Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D014404:Tubulin; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases