Oxidative DNA base damage in lymphocytes of HIV-infected drug users.
In the present study, we have studied the level of oxidative DNA base damage in lymphocytes of HIV-infected intravenous drug users (IDUs) and a seronegative control group. Chromatin was isolated from the lymphocytes and then analyzed by gas chromatography/isotope-dilution mass spectrometry with selected-ion monitoring (GC/IDMS-SIM). Significantly greater levels of four oxidatively modified DNA bases were observed in chromatin samples from the symptomatic HIV-infected patients than in those from the seronegative patients. These were 5-hydroxyuracil, 5-hydroxycytosine, 8-hydroxyadenine and 8-hydroxyguanine. In the case of 5-hydroxyuracil and 8-hydroxyguanine, a statistically significant difference was also found between the control group and the asymptomatic HIV-positive patients. These results suggest that oxidative stress may play an important role in the pathogenesis of acquired immune deficiency syndrome (AIDS), and that administration of antioxidant drugs to HIV-infected patients may offer protection against AIDS-related carcinogenesis.
Jaruga
P
P
Department of Clinical Biochemistry, The Ludwik Rydygier Medical University, Bydgoszcz, Poland. pjaruga@aciamb.bydgoszcz.pl
Jaruga
B
B
Olczak
A
A
Halota
W
W
Olinski
R
R
D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D000293:Adolescent; D000328:Adult; D002843:Chromatin / Q000235:genetics; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004249:DNA Damage / Q000235:genetics*; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D015658:HIV Infections / Q000097:blood* / Q000150:complications; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000821:virology; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D015819:Substance Abuse, Intravenous / Q000097:blood* / Q000150:complications; D014498:Uracil / Q000031:analogs & derivatives / Q000032:analysis
D002843:Chromatin; C002992:8-hydroxyadenine; C045975:5-hydroxyuracil; C024829:8-hydroxyguanine; D014498:Uracil; D006147:Guanine; C017400:5-hydroxycytosine; D003596:Cytosine; D000225:Adenine