Inflammatory responses to amyloid fibrils.
Our laboratory has routinely used the methodologies described here to characterize the effects of fibrillar A beta and amylin on cytokine synthesis and secretion by LPS-differentiated THP-1 cells. Because LPS-treated THP-1 cells resemble macrophage and microglia, this assay system represents an in vitro model of the potential interactions between A beta-containing senile plaques and microglia in the AD brain. As such, these methodologies should prove useful in the identification of compounds that inhibit this A beta-induced inflammatory response.
Yates
S L
SL
Gliatech Inc., Cleveland, Ohio 44122, USA.
Kocsis-Angle
J
J
Embury
P
P
Brunden
K R
KR
D000682:Amyloid / Q000235:genetics / Q000276:immunology*; D016229:Amyloid beta-Peptides / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D058228:Islet Amyloid Polypeptide; D009000:Monocytes / Q000276:immunology*; D011994:Recombinant Proteins / Q000378:metabolism
D000682:Amyloid; D016229:Amyloid beta-Peptides; D007375:Interleukin-1; D058228:Islet Amyloid Polypeptide; D011994:Recombinant Proteins