HLA-DR polymorphism influences T-cell precursor frequencies to Epstein-Barr virus (EBV) gp110: implications for the association of HLA-DR antigens with rheumatoid arthritis.
To study whether HLA-DR haplotypes associated with susceptibility to develop rheumatoid arthritis (RA) may influence T-cell responses to the Epstein-Barr virus (EBV) gp110 (a protein of the late replicative cycle of EBV), we evaluated the frequency in peripheral blood of T cells capable to proliferate to EBV gp110 by direct limiting dilution analysis in 50 HLA-DR-typed healthy subjects. NVe found that HLA-DRB1*07, an allele associated with reduced risk to develop RA, is associated with the highest frequencies of T cells specific for gp110 in peripheral blood. In contrast, HLA-DRB1*0404, one of the susceptibility alleles is associated with the lowest frequencies of gp110 specific T cells. Finally, people expressing both HLA-DRB1*07 and HLA-DRB1*0404 display low precursor frequencies to EBV gp110.
Toussirot
E
E
Service de Rhumatologie, Hopital Minjoz, Besançon, France.
Auger
I
I
Roudier
C
C
Luka
J
J
Wendling
D
D
Tiberghien
P
P
Roudier
J
J
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology*; D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D002144:Callithrix; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D006224:Cricetinae; D003412:Cricetulus; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D018655:Lymphocyte Count; D011110:Polymorphism, Genetic / Q000235:genetics* / Q000276:immunology; D013234:Stem Cells / Q000166:cytology / Q000821:virology*; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology*; D014764:Viral Proteins / Q000276:immunology*
C060207:Epstein-Barr virus glycoprotein gp110; D006684:HLA-DR Antigens; D014764:Viral Proteins