title: Selection of human anti-HAV McAb from a phage antibody library.
abstract: The phage displaying antibody fragments were subjected to three rounds of panning with hepatitis A virus (HAV) antigen in solid phase. The eluted phage was enriched nearly 100 fold, and the percentage of recombinant clones increased from 25% to 100% after three rounds of panning. The HAV antigen-specific monoclonal antibody (McAb) was screened by sandwich ELISA, and the specificity of these antibodies was further confirmed by competitive inhibition ELISA.
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wan
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D019151:Peptide Library; D015183:Restriction Mapping
chemical_list: D000911:Antibodies, Monoclonal; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D019151:Peptide Library