Design and scale up of a process for manganese peroxidase production using the hypersecretory strain Phanerochaete chrysosporium I-1512.
Manganese peroxidase (MnP) production was performed in a airlift bioreactor in which Phanerochaete chrysosporium I-1512, an MnP hypersecretory strain, was immobilized on a stainless steel mesh. Production was scaled up from a 2.5-L bench scale to a 100-L bioreactor. The yield of MnP was increased 2-fold and reached 6600 U L(-1). These results indicate the feasibility of MnP production on a medium scale, which promises sufficient MnP availability for its use in pulp bleaching at industrial scale.
Herpoël
I
I
Laboratoire de Biotechnologie des Champignons Filamenteux, INRA, Centre d'Enseignement Supérieur en Biotechnologie, ESIL, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France.
Asther
M
M
Sigoillot
J C
JC
D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D005990:Glycerol / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D020075:Phanerochaete / Q000235:genetics / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D005990:Glycerol