Expression of 15N-labeled eukaryotic cytochrome c in Escherichia coli.
We present a simple and inexpensive method for producing 15N-labeled Saccharomyces cerevisiae iso-1-cytochrome c in Escherichia coli. The labeled protein gives excellent NMR spectra.
Morar
A S
AS
Department of Chemistry, University of North Carolina at Chapel Hill 27599, USA.
Kakouras
D
D
Young
G B
GB
Boyd
J
J
Pielak
G J
GJ
D003574:Cytochrome c Group / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D003574:Cytochrome c Group; D009587:Nitrogen Isotopes