Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts.
Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.
Danesh
F R
FR
Division of Nephrology/Hypertension, Northwestern University Medical School and Chicago VA Healthcare System, Lakeside Division, Chicago, Illinois 60611, USA.
Ye
M
M
Salmi
S
S
Lapointe
M
M
Batlle
D
D
D001798:Blood Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016193:G1 Phase / Q000502:physiology; D006801:Humans; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects / Q000502:physiology*; D012867:Skin / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors; D014316:Tritium
D001798:Blood Proteins; D016160:Retinoblastoma Protein; D014316:Tritium; D019938:Cyclin D1; D013936:Thymidine