The small GTP-binding protein rac is a regulator of cell death in plants.
Cell death plays important roles in the development and defense of plants as in other multicellular organisms. Rapid production of reactive oxygen species often is associated with plant defense against pathogens, but their molecular mechanisms are not known. We introduced the constitutively active and the dominant negative forms of the small GTP-binding protein OsRac1, a rice homolog of human Rac, into the wild type and a lesion mimic mutant of rice and analyzed H(2)O(2) production and cell death in transformed cell cultures and plants. The results indicate that Rac is a regulator of reactive oxygen species production as well as cell death in rice.
Kawasaki
T
T
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Henmi
K
K
Ono
E
E
Hatakeyama
S
S
Iwano
M
M
Satoh
H
H
Shimamoto
K
K
D000595:Amino Acid Sequence; D017209:Apoptosis; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000502:physiology*; D005799:Genes, Dominant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008969:Molecular Sequence Data; D012275:Oryza / Q000033:anatomy & histology; D010940:Plant Proteins / Q000737:chemistry* / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D019204:GTP-Binding Proteins