Genome analysis of several marine, magnetotactic bacterial strains by pulsed-field gel electrophoresis.
The genomic DNA of three strains of marine magnetotactic bacteria, including two facultatively anaerobic vibrios, strains MV-1 and MV-2, and the microaerophilic coccus, strain MC-1, was analyzed by pulsed-field gel electrophoresis (PFGE). Digestion of the genomic DNA of strain MV-1 by the restriction endonucleases AvrII, BamHI, HindIII, NheI, SalI, SfiI, SgfI, SgrAI, and XbaI resulted in a large number of fragments below 400 kb that were difficult to resolve by PFGE. Digestion of MV-1 DNA with NotI and RsrII resulted in no fragments. Treatment of genomic DNA of strains MV-1 and MV-2 with PacI, PmeI, and SpeI yielded a manageable number of fragments (ca. 20) that were relatively easily resolved with PFGE, while PacI and SpeI were effective for strain MC-1. There was no evidence for the presence of plasmids and linear chromosomes in any of the strains, and strains MV-1 and MV-2 appear to contain a single, circular chromosome. Genome sizes of strains MV-1, MV-2, and MC-1 were estimated to be between 3.6 and 3.9 Mb (mean +/- SD; 3.7 +/- 0.2), 3. 3 and 3.7 Mb (3.6 +/- 0.2), and 4.3 and 4.7 Mb (4.5 +/- 0.3), respectively. The restriction fragment patterns of the vibrioid strains MV-1 and MV-2 were extremely similar, suggesting that the strains are closely related.
Dean
A J
AJ
Department of Microbiology, 207 Science I, Iowa State University, Ames, IA 50011, USA.
Bazylinski
D A
DA
D004262:DNA Restriction Enzymes / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000254:growth & development; D008280:Magnetics; D014871:Water Microbiology
D004262:DNA Restriction Enzymes