title: Molecular cytogenetic identification of cyclin D1 gene amplification in a renal pelvic tumor attributed to phenacetin abuse.
abstract: Despite extensive epidemiologic evidence of phenacetin abuse as a risk factor for renal pelvic carcinomas, genetic alterations in the resultant tumors remain largely unclear. In this report, a phenacetin-associated renal pelvic carcinoma (histologically a transitional-cell carcinoma) from an 80-year-old female patient was evaluated by molecular cytogenetic methods. Fluorescence in situ hybridization was used to identify chromosome gains or losses for the cyclin D1, p53, Rb and c-myc genes and the ploidy of their respective chromosomes. Cyclin D1 gene amplification, but normal copy numbers of p53, Rb and c-myc, and normal ploidy of chromosomes 8, 11, 13 and 17 were observed. Expression of cyclin D1 protein was confirmed by immunohistochemistry. In the absence of p53, Rb or c-myc abnormalities, the results suggested that cyclin D1 gene amplification and its protein overexpression may be involved in the genesis of renal pelvic carcinomas associated with phenacetin abuse.
authors.affiliation: First Department of Pathology, Osaka City University Medical School, Nagoya, Japan.
authors.forename: C C
authors.initials: CC
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Wanibuchi
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Hirose
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: S
authors.initials: S
authors.lastname: Fukushima
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018712:Analgesics, Non-Narcotic / Q000009:adverse effects*; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D002877:Chromosomes, Human / Q000235:genetics; D019938:Cyclin D1 / Q000032:analysis; D017809:Fatal Outcome; D005260:Female; D016161:Genes, Retinoblastoma / Q000235:genetics; D019940:Genes, bcl-1 / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007680:Kidney Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D007682:Kidney Pelvis / Q000187:drug effects* / Q000473:pathology; D010615:Phenacetin / Q000009:adverse effects*; D011003:Ploidies
chemical_list: D018712:Analgesics, Non-Narcotic; D019938:Cyclin D1; D010615:Phenacetin