[Expressions of Bcl-2, Bax and Bak proteins in liver tissues of hepatitis B patients and their significance].
OBJECTIVE
To investigate the expressions of Bcl-2, Bax and Bak proteins in liver tissues of hepatitis B patients and their relations to apoptosis and necrosis of hepatocytes.


METHODS
TUNEL assay and immunohistochemistry were used to detect hepatic apoptosis, Bcl-2, Bax and Bak proteins expressions on hepatocytes respectively.


RESULTS
In liver cirrhosis group(10 cases), acute hepatitis group(8 cases), chronic hepatitis group (55 cases) and hepatic failure group(16 cases), the moderate positive rates of TUNEL assay were 30.0%, 25.0%, 61.8%, and 93.8% respectively; the moderate positive rates of Bcl-2 oncoprotein were 30.0%, 25.0%, 38.2% and 12.5% respectively; the moderate positive rates of Bax protein were 20.0%, 25.0%, 47.3% and 87.5% respectively; the moderate positive rates of Bak protein were 10.0%, 12.5%, 25.5% and 75.0% respectively. There was a positive correlation between degrees of the expression of Bax, Bak and hepatic apoptosis (P < 0.05). The expression of Bcl-2 protein on hepatocytes in proliferation zones was stronger than in necrosis zones.


CONCLUSIONS
The enhanced degrees of the expression of Bax and Bak proteins were accompanied with the enhanced degrees of the necrosis.
Zhang
B
B
Institute of Viral Hepatitis, Chongqing University of Medical Sciences.
Zhang
D
D
Ma
Y
Y
D000328:Adult; D000368:Aged; D002455:Cell Division; D005260:Female; D006509:Hepatitis B / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008875:Middle Aged; D009336:Necrosis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein
C494772:BAK1 protein, human; C494826:BAX protein, human; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein