title: Proteolytic cleavage of the repressor (BlaI) of beta-lactamase synthesis in Staphylococcus aureus.
abstract: The proteolytic cleavage of BlaI was shown to correlate with beta-lactamase synthesis in Staphylococcus aureus. BlaI was found to be autoregulatory when expressed from the blaZ promoter. Insertion of a 10-bp linker into the SnaBI site of blaRI resulted in constitutive synthesis of beta-lactamase.
authors.affiliation: Department of Biochemistry, University of Oxford, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Lewis
authors.forename: S P
authors.initials: SP
authors.lastname: Curnock
authors.forename: K G
authors.initials: KG
authors.lastname: Dyke
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D001618:beta-Lactamases