Hepatic production of apolar aldehydes in rats with carbon tetrachloride-induced cirrhosis.
The aim of this study was to identify apolar aldehydes in liver homogenates from rats with CCl4-induced cirrhosis and, as a corollary, the antioxidant effect of zinc administration. The study was performed in five control rats and in ten cirrhotic rats which were further sub-divided into two groups to receive either a standard diet or one supplemented with zinc. The percentage of hepatic fibrosis, plasma malondialdehyde concentration and alanine aminotransferase activity were measured as well as the following aldehydes: hexanal, octanal, decanal, 2-hexenal, 2-octenal, 2-nonenal, 2,4-heptadienal and 2,4-decadienal. Of the 10 cirrhotic rats, 4 had elevated concentrations of the highly toxic 2,4-dialkenals which coincided with a higher percentage of fibrosis and plasma alanine aminotransferase activity. These aldehydes were not observed in the control group. Zinc administration was associated with a reduction of the hepatic malondialdehyde concentration and an amelioration on the degree of hepatic injury. In conclusion, this study demonstrates the presence of the highly toxic 2,4-dialkenals in hepatic tissue of rats whith CCl4-induced cirrhosis. Results obtained would suggest that these particular aldehydes may be related to the severity of the hepatic injury.
Ferré
N
N
Unitat Clínico-Experimental d'Investigació, Hospital Universitari de Sant Joan-Facultat de Medicina, Universitat Rovira i Virgili, Reus, Catalunya, Spain.
Girona
J
J
Cabré
M
M
Joven
J
J
LaVille
A
A
Masana
L
L
Paternáin
J L
JL
Camps
J
J
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000494:pharmacology
D000447:Aldehydes; D000975:Antioxidants; D002251:Carbon Tetrachloride; D015032:Zinc