Overlapping sequences with high homology to functional proteins coexist on complementary strands of DNA in the rumen bacterium Prevotella albensis.
The potential for two complementary fragments of DNA from a clone from the ruminal bacterium Prevotella albensis to encode sequences with homology to at least part of functional proteins is described. One strand contains a sequence with high homology to dnaK, a member of the hsp70 family, and the other strand contains a sequence with some homology to glutamate dehydrogenase genes. Overlapping of these two genes on opposite strands has been reported in eukaryotic species, and is now reported for the first time in a bacterial species. Further investigation of previously described dnaK genes demonstrates that it is more widespread than might be anticipated, with all thirty other dnaK genes investigated also retaining long sequences encoding at least part of a sequence with high homology to a glutamate dehydrogenase gene.
Walker
N D
ND
Rowett Research Institute, Greenburn Road, Aberdeen, Bucksburn, AB21 9SB, Scotland.
McEwan
N R
NR
Wallace
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D029968:Escherichia coli Proteins; D005969:Glutamate Dehydrogenase / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D018720:Prevotella / Q000235:genetics*; D012417:Rumen / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D016373:DNA, Antisense; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D005969:Glutamate Dehydrogenase; C063842:dnaK protein, E coli