title: Development of an experimental ovarian tumor over a year in the rat.
abstract: Tumor growth, possible malignant transformation or metastatic propagation and hormonal patterns were evaluated over a year in luteoma induced by introducing an ovary into the spleen of ovariectomized 60 day-old rats. Sham castrated animals had a piece of muscle inserted into the spleen. Jugular blood samples were taken monthly. After a year animals were cycled and decapitated. Troncal blood was collected, autopsies were performed and luteoma were measured and fixed in 10% buffered formalin. Serum LH, FSH, PRL, estradiol and progesterone were measured. Serum inhibin content was determined in one month-old tumors-bearing animals and estrous rats as controls. After one year no external changes in tumor-bearing rats were observed, nor differences in body weight or mortality rates compared to Sham animals. Metastatic propagation was absent. Routine histological examination showed two types of tumors according to either granulosa or luteal cell predomination, tumor type did not determine hormonal patterns. However, a clear relationship between gonadotropin levels and tumor size was established. Low gonadotropins: Small tumors, 18.7% of cases and high gonadotropins: Large tumors, 81.3%. In Sham animals gonadotropins attained castrate levels and remained elevated until the end of the experiment. In the Small group no increases in gonadotropins or estradiol were detected, progesterone and PRL fluctuated. In the Large tumor group LH increased to Sham titers until month 7, then fell to initial levels, FSH augmented significantly as from month three and remained high up to month 5. No variations in either estradiol, progesterone or PRL were observed. Serum inhibin of one month-old tumor-bearing rats was significantly elevated, justifying the lack of FSH increase at this time point. We conclude that these luteoma do not suffer malignant transformation or induce metastases. They appear in two histological types. Tumor size depends on hormonal patterns. The delay in the initial increase and the sharp decrease observed in FSH in animals bearing Large tumors suggest a possible role for inhibin in this regulation.
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental-CONICET, Dep. Fisiología, Fac. Medicina, Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Chamson-Reig
authors.forename: M S
authors.initials: MS
authors.lastname: Bianchi
authors.forename: E
authors.initials: E
authors.lastname: Rey-Roldán
authors.forename: E
authors.initials: E
authors.lastname: Sorianello
authors.forename: C
authors.initials: C
authors.lastname: Libertun
authors.forename: V
authors.initials: V
authors.lastname: Lux-Lantos
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D012739:Gonadal Steroid Hormones / Q000097:blood; D007265:Inhibins / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D011388:Prolactin / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012739:Gonadal Steroid Hormones; D007265:Inhibins; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone