title: Neutrophil infiltration into human gliomas.
abstract: Human gliomas were analysed for the infiltration of neutrophils using immunohistochemistry by staining sections for CD15-positive and myeloperoxidase-positive cells. Over 70% of all glioma samples analysed (n = 105) had significant neutrophil infiltration, but there was a marked and significant correlation between tumour grade and the extent of the neutrophil infiltration. In the low grade tumours only 40-50% had significant infiltration, while in glioblastoma multiforme over 85% of the samples analysed had significant infiltration. Numbers of neutrophils infiltrating glioblastoma multiforme tumours were also greater than in the other tumour groups. Circulating white blood cell counts were elevated above the normal range in all glioma patients, but this elevation was entirely due to increased numbers of circulating neutrophils. Again, the highest numbers of circulating neutrophils were seen in the glioblastoma multiforme patients. These experiments indicate that glioma-derived factors may directly or indirectly affect the number of circulating neutrophils and influence their infiltration into the tumours.
authors.affiliation: Walton Centre for Neurology and Neurosurgery, Liverpool, UK.
authors.forename: G
authors.initials: G
authors.lastname: Fossati
authors.forename: G
authors.initials: G
authors.lastname: Ricevuti
authors.forename: S W
authors.initials: SW
authors.lastname: Edwards
authors.forename: C
authors.initials: C
authors.lastname: Walker
authors.forename: A
authors.initials: A
authors.lastname: Dalton
authors.forename: M L
authors.initials: ML
authors.lastname: Rossi
mesh_terms: D001853:Bone Marrow / Q000473:pathology; D001932:Brain Neoplasms / Q000098:blood supply / Q000473:pathology*; D016923:Cell Death / Q000502:physiology; D005909:Glioblastoma / Q000098:blood supply / Q000473:pathology; D005910:Glioma / Q000098:blood supply / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007958:Leukocyte Count; D009336:Necrosis; D009389:Neovascularization, Pathologic / Q000473:pathology; D020556:Neutrophil Infiltration / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology