Lymphocyte beta -adrenoceptor downregulation in great danes with occult dilated cardiomyopathy (DCM) and with DCM and heart failure.
The importance of the adrenergic nervous system in supporting the failing heart has long been known. The adrenergic drive on cardiac structure and function has however some adverse effects, which include myocardial beta-adrenoceptor (beta-AR) downregulation and decreased beta-adrenergic responsiveness to cathecolamines. In dog lymphocytes, beta(1)-AR and beta(2)-AR populations are almost equally represented (with a slight prevalence of beta(2)) and a significant correlation between cardiac and lymphocytic adrenoceptors has been found. The aim of the present study was to investigate possible differences between the concentration of lymphocytic beta-AR in healthy dogs, dogs with dilated cardiomyopathy (DCM) and dogs with occult DCM. Three groups of great danes were considered: a control group (n =10), dogs with DCM (n =9) and dogs with occult DCM (n =4). Lymphocytic beta-AR populations were determined in all dogs. A substantial and significant decrease (P<0.05) in total-AR, beta(1)-AR and beta(2)-AR concentrations in the lymphocytes of dogs with symptomatic DCM and occult DCM compared to the control group was found. Although the mean value of the lymphocyte beta(1)-AR number in the asymptomatic group was double compared to the DCM group, this difference was not statistically significant. We conclude that in dogs beta-AR downregulation occurs early in the course of dilated cardiomyopathy. This finding may suggest the value of early use of a beta-blocker in the therapeutic regimen. Moreover, the continuous monitoring of lymphocytic beta-AR may represent a useful tool for the development of a more effective individual therapy.
Borgarelli
M
M
Department of Animal Pathology, Division of Veterinary Medicine, Via Nizza 52, Torino, I-10126, Italy.
Badino
P
P
Bergamasco
L
L
Bussadori
C
C
Odore
R
R
Re
G
G
Tarducci
A
A
Dotta
U
U
D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000503:physiopathology*; D004285:Dogs; D015536:Down-Regulation; D004562:Electrocardiography / Q000662:veterinary; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D018342:Receptors, Adrenergic, beta-1 / Q000032:analysis / Q000097:blood* / Q000737:chemistry; D012016:Reference Values
D018342:Receptors, Adrenergic, beta-1