Versatile fluorescence labeling method using activated esters on solid support.
A versatile fluorescence labeling method employing support bound nitrophenyl, N-hydroxysuccinimidyl, 1-hydroxybenzotriazoyl and oxime active esters is described. The relative kinetic properties of different esters were systematically compared using an environment-independent benzoquinoline dye.
Chang
Y T
YT
Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037, USA.
Schultz
P G
PG
D002851:Chromatography, High Pressure Liquid; D004952:Esters / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004952:Esters; D005456:Fluorescent Dyes