title: Interpretation of intrinsic optical signals and calcein fluorescence during acute excitotoxic insult in the hippocampal slice.
abstract: Immediate (acute) neuronal damage in response to overstimulation of glutamate receptors results from toxic exposure to food poisons acting as glutamate analogues. Glutamate agonist application evokes dramatic intrinsic optical signals (IOSs) in the rat hippocampal slice preparation, particularly in the CA1 region. Theoretically IOSs are generated by alterations to neuronal and glial structure that change light transmittance (LT) in live brain tissue. To better understand such signals, IOSs evoked by the glutamate agonist N-methyl-D-aspartate were imaged in the rat hippocampal slice. We correlated these excitotoxic signals with: (1) biophysical principles governing light transport, (2) tissue volume changes as measured using a free intracellular fluorophore (calcein), (3) dendritic morphology visualized by dye injection, and (4) standard histopathology. In theory LT elevation evoked during acute excitotoxic swelling is generated by change to subcellular structure that reduces light scattering during cell swelling. However, in responsive dendritic regions, initial LT elevation caused by cell swelling was overridden by the formation of dendritic beads, a conformation that increased light scattering (thereby reducing LT) even as the calcein signal demonstrated that the tissue continued to swell. Thus IOS imaging reveals acute somatic and dendritic damage during excitotoxic stress that can be monitored across slices of brain tissue in real time.
authors.affiliation: Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
authors.forename: C R
authors.initials: CR
authors.lastname: Jarvis
authors.forename: L
authors.initials: L
authors.lastname: Lilge
authors.forename: G J
authors.initials: GJ
authors.lastname: Vipond
authors.forename: R D
authors.initials: RD
authors.lastname: Andrew
mesh_terms: D000818:Animals; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005665:Furosemide / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008297:Male; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D016202:N-Methylaspartate / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D009498:Neurotoxins / Q000633:toxicity*; D017966:Pyramidal Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D009498:Neurotoxins; C085925:calcein AM; D016202:N-Methylaspartate; D005665:Furosemide