Heterologous expression and product identification of Colletotrichum lagenarium polyketide synthase encoded by the PKS1 gene involved in melanin biosynthesis.
The Colletotrichum lagenarium PKS1 gene was expressed in the heterologous fungal host, Aspergillus oryzae, under the starch-inducible alpha-amylase promoter to identify the direct product of polyketide synthase (PKS) encoded by the PKS1 gene. The main compound produced by an A. oryzae transformant was isolated and characterized to be 1,3,6,8-tetrahydroxynaphthalene (T4HN) as its tetraacetate. Since the PKS1 gene was cloned from C. lagenarium to complement the nonmelanizing albino mutant, T4HN was assumed to be an initial biosynthetic intermediate, and thus the product of the PKS reaction, but had not been isolated from the fungus. The production of T4HN by the PKS1 transformant unambiguously identified the gene to encode a PKS of pentaketide T4HN. In addition, tetraketide orsellinic acid and pentaketide isocoumarin were isolated, the latter being derived from a pentaketide monocyclic carboxylic acid, as by-products of the PKS1 PKS reaction. Production of the pentaketide carboxylic acid provided insights into the mechanism for the PKS1 polyketide synthase reaction to form T4HN.
Fujii
I
I
Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
Mori
Y
Y
Watanabe
A
A
Kubo
Y
Y
Tsuji
G
G
Ebizuka
Y
Y
D001236:Aspergillus oryzae / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D020231:Colletotrichum / Q000737:chemistry*; D005656:Fungal Proteins / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal; D005815:Genetic Code; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins / Q000096:biosynthesis; D009097:Multienzyme Complexes / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics; D010940:Plant Proteins; D014170:Transformation, Genetic
D002352:Carrier Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009097:Multienzyme Complexes; C119522:PKS1 protein, plant; D010750:Phosphoproteins; D010940:Plant Proteins