title: [Association of MAP kinases with the endoplasmic reticulum in NIH3T3 (HER14) and A431 cells].
abstract: Intracellular distribution of p42/p44 MAP-kinases in HER14 and A431 cell lines was investigated. Using subcellular fractionation and immunofluorescence approaches we have shown that in quiescent cells of both types MAP-kinases are associated with endoplasmic reticulum. Moreover, ER-localized MAP-kinases were shown to exist only in a nonphosphorylated form. In HER14 cells the epidermal growth factor (EGF) elevates the level of the ER-associated MAP-kinases. In contrast, exposure of A431 cells to EGF leads to a significant decrease in the observed association. The physiological role of this association is discussed.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
authors.forename: A M
authors.initials: AM
authors.lastname: Arnautov
authors.forename: I P
authors.initials: IP
authors.lastname: Arnautova
authors.forename: N N
authors.initials: NN
authors.lastname: Zheleznova
authors.forename: E S
authors.initials: ES
authors.lastname: Kornilova
authors.forename: N N
authors.initials: NN
authors.lastname: Nikol'skiĭ
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D051379:Mice; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured
chemical_list: D004815:Epidermal Growth Factor; D017871:Calcium-Calmodulin-Dependent Protein Kinases