title: Clinical scale isolation of highly purified peripheral CD34+progenitors for autologous and allogeneic transplantation in children.
abstract: We present our experience with three clinical scale isolation methods for positive selection of CD34+ progenitors from peripheral blood for autologous and allogeneic transplantation in children. A combination of the CellPro device and the Magnetic Activated Cell Sorting system (MACS), as well as two different combinations of MACS systems were used (VarioMACS-SuperMACS and SuperMACS-SuperMACS). With the CellPro-MACS combination (16 separations), a median purity of 96.2% and a median recovery of 42% CD34+ cells could be achieved, whereas the two step MACS systems (55 and 29 separations) showed a median purity of 97.6% and 98.0% and a median recovery of 96.5% and 97%, respectively. Depletion of T cells was profound (4-5 log). A total of 34 patients in the autologous and 18 patients in the allogeneic setting have been transplanted with highly enriched CD34+ cells, obtained by these methods. Only one patient failed to engraft, all other patients showed a rapid and sustained hematological engraftment with the longest follow-up of 3 years. In summary, especially the two step MACS systems have proven to be appropriate tools for enrichment of CD34+ cells, yielding both high purity and good recovery, and can thus be used for tumor cell purging in the autologous setting and for effective T cell depletion in the allogeneic setting.
authors.affiliation: Children's University Hospital, University of Tuebingen, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Lang
authors.forename: M
authors.initials: M
authors.lastname: Schumm
authors.forename: G
authors.initials: G
authors.lastname: Taylor
authors.forename: T
authors.initials: T
authors.lastname: Klingebiel
authors.forename: S
authors.initials: S
authors.lastname: Neu
authors.forename: A
authors.initials: A
authors.lastname: Geiselhart
authors.forename: S
authors.initials: S
authors.lastname: Kuci
authors.forename: D
authors.initials: D
authors.lastname: Niethammer
authors.forename: R
authors.initials: R
authors.lastname: Handgretinger
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018189:Immunomagnetic Separation; D007223:Infant; D008875:Middle Aged; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D018952:Antigens, CD34