Changes in the distribution of peanut agglutinin (PNA) binding molecules during muscle reinnervation following nerve crush injury.
Peanut agglutinin (PNA) staining during muscle reinnervation following a crushing injury of the sciatic nerve was performed in reference to the neural profiles immunolabeled with the PGP 9.5 antibody. PNA staining in the normal controls exhibited dots, granules, or lines along the length of the nerve fibers in the nerve trunk, but was faint or absent in the motor endplate. At seven days post-crush, PNA staining was detected around the vacuolated neural structures in the disorganized nerve trunk, but was still faint or absent in the motor endplate. At twenty-one days post-crush, when PGP 9.5-positive regenerating axons appeared in most of the motor endplates, PNA staining, either faint or strong, followed the pathway of the nerve fibers delineated by PGP 9.5-like immunoreactivity. During reinnervation to the motor endplates, PNA staining displayed signs of remodeling in the nerve trunk, such as marked variations in density and profile in the nerve fiber-associated dots or patches; it increased in intensity in the connective tissue covering the area of the motor endplate, as well as in the junctional myofiber surface. The structures recognizable by PNA coincided with components of the connective tissue such as collagen fibers and capillaries. Results suggest that: 1) the expression of PNA-binding molecules is dependent on the state of innervation, and 2) the spatiotemporal relationship between neural profiles and PNA staining provides sequences of axonal extension and subsequent nerve terminal maturation during regeneration in the motor endplate.
Zhou
C J
CJ
Department of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Kawabuchi
M
M
He
J W
JW
Kuraoka
A
A
Hirata
K
K
Wang
S
S
Nada
O
O
D000818:Animals; D003238:Connective Tissue / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009045:Motor Endplate / Q000502:physiology; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation* / Q000502:physiology; D009412:Nerve Fibers / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000276:immunology; D019887:Peanut Agglutinin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000254:growth & development / Q000293:injuries* / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000276:immunology; D013997:Time Factors; D043222:Ubiquitin Thiolesterase; D014617:Vacuoles
D006001:Glycoconjugates; D009419:Nerve Tissue Proteins; D019887:Peanut Agglutinin; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase; D005690:Galactose