Characterization of 3-methoxyflavones using fast-atom bombardment and collision-induced dissociation tandem mass spectrometry.
A mass spectrometric method based on the combined use of fast-atom bombardment (FAB), collision-induced dissociation (CID) and linked scanning at constant B/E has been used for the analysis of the fragmentation behavior of protonated 3-methoxyflavones (3-MFs). It is shown that several diagnostic ions such as (1,3)A (+) and (0,2)B (+) ions allow for an unambiguous localization of functions in the A- and B-rings and that the position of an additional methoxy group in the B-ring could be determined by a detailed analysis of the spectral patterns. Isomeric 3-MFs can be differentiated using this methodology. General fragmentation patterns of 3-MFs are discussed and plausible formation mechanisms of relatively abundant fragment ions are proposed.
Ma
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University of Antwerp (UIA), Department of Pharmaceutical Sciences, Universiteitsplein 1, B-2610 Antwerp, Belgium.
Heuvel
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HV
Claeys
M
M
D005419:Flavonoids / Q000737:chemistry*; D007536:Isomerism; D015394:Molecular Structure; D016339:Spectrometry, Mass, Fast Atom Bombardment
D005419:Flavonoids