title: Kaolin-induced hydrocephalus in the hamster: temporal sequence of changes in intracranial pressure, ventriculomegaly and whole-brain specific gravity.
abstract: The development of kaolin-induced hydrocephalus in adult hamsters was monitored by measuring changes in intracranial pressure (ICP), ventriculomegaly (VG) and whole-brain specific gravity (SG). Controls were intact or sham operated animals. Relative to controls, ICP of experimental animals increased at 24 h post intracisternal kaolin injection (by approximately 7-fold), reached a maximum on day 6 (by approximately 12-fold) and remained markedly elevated through day 15 (by approximately 5-fold). Ventricles differed in time of onset of distension (third: day 1, lateral: day 2, fourth: day 4) and in time of maximum ventriculomegaly (fourth: day 6; third: day 7; and lateral: day 9). Ventricular distension resulted in alterations in the ependyma; cilia were lost and apical cell surfaces were distorted. The ependyma was ruptured and the subjacent neuropil was exposed to the cerebrospinal fluid in some regions. Whole-brain SG remained constant in controls but declined in hydrocephalic hamsters after day 3 post-kaolin injection and reached its nadir on day 9 when whole-brain water content was 18% greater than in controls. Consistent with the fact that causal relationships exist between increased ICP, ventricular distension and brain edema, the alterations in each parameter occurred sequentially rather than simultaneously, and the time-course of each manifestation of hydrocephalus differed. The data suggest that the pathophysiology of kaolin-induced hydrocephalus in the hamster is tri-phasic: an initial period of rapid change, a brief interval of maximum alteration, and a subsequent period of compensation.
authors.affiliation: Department of Anatomy/Cell Biology, Wayne State University, School of Medicine, Detroit, MI 48201-1998, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Azzi
authors.forename: A I
authors.initials: AI
authors.lastname: Canady
authors.forename: S
authors.initials: S
authors.lastname: Ham
authors.forename: J A
authors.initials: JA
authors.lastname: Mitchell
mesh_terms: D000367:Age Factors; D000818:Animals; D002923:Cilia / Q000473:pathology; D006224:Cricetinae; D004195:Disease Models, Animal; D004805:Ependyma / Q000473:pathology* / Q000503:physiopathology* / Q000648:ultrastructure; D005260:Female; D006849:Hydrocephalus / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D007427:Intracranial Pressure; D007616:Kaolin; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D010592:Pharmaceutic Aids; D013046:Specific Gravity; D013346:Subarachnoid Space / Q000473:pathology
chemical_list: D010592:Pharmaceutic Aids; D007616:Kaolin