Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH.
We previously reported findings that NADPH/cytochrome P450 reductase can generate superoxide anion radical (O2*-) from heterocyclic amines (HCA) and from many anticancer agents in vitro. Here we present more evidence in which O2*- is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro. This indicates that free radical generation by rh-Cytb5Rd in the presence of HCA may add new insight into the damage of DNA in addition to the previously known mechanism: interaction of activated HCA-intermediates to form DNA adduct.
Maeda
H
H
Department of Microbiology, Kumamoto University School of Medicine, Japan. msmaedah@gpo.kumamoto-u.ac.jp
Sawa
T
T
Yubisui
T
T
Akaike
T
T
D003579:Cytochrome Reductases / Q000378:metabolism*; D042966:Cytochrome-B(5) Reductase; D004249:DNA Damage; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D009243:NAD / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism*; D011810:Quinoxalines / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D005609:Free Radicals; D011804:Quinolines; D011810:Quinoxalines; D011994:Recombinant Proteins; D009243:NAD; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; C036990:2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase