title: Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC.
abstract: Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different beta-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC, beta-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.
authors.affiliation: Center for Research in Anti-Infectives and Biotechnology, Department of Pediatrics, Creighton University School of Medicine and Methodist Hospital, Omaha, NE 68178, USA. ndhanson@creighton.edu
authors.forename: N D
authors.initials: ND
authors.lastname: Hanson
authors.forename: K S
authors.initials: KS
authors.lastname: Thomson
authors.forename: E S
authors.initials: ES
authors.lastname: Moland
authors.forename: C C
authors.initials: CC
authors.lastname: Sanders
authors.forename: G
authors.initials: G
authors.lastname: Berthold
authors.forename: R G
authors.initials: RG
authors.lastname: Penn
mesh_terms: D000328:Adult; D001426:Bacterial Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics*; D008297:Male; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases