title: Chip and apterous physically interact to form a functional complex during Drosophila development.
abstract: LIM homeodomain (LIM-HD) proteins play key roles in a variety of developmental processes throughout the animal kingdom. Here we show that the LIM-binding protein Chip acts as a cofactor for the Drosophila LIM-HD family member Apterous (Ap) in wing development. We define the domains of Chip required for LIM-HD binding and for homodimerization and show that mutant proteins deleted for these domains act in a dominant-negative fashion to disrupt Ap function. Our results support a model for multimeric complexes containing Chip and Ap in transcriptional regulation. This model is confirmed by the activity of a chimeric fusion between Chip and Ap that reconstitutes the complex and rescues the ap mutant phenotype.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California 92037, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: van Meyel
authors.forename: D D
authors.initials: DD
authors.lastname: O'Keefe
authors.forename: L W
authors.initials: LW
authors.lastname: Jurata
authors.forename: S
authors.initials: S
authors.lastname: Thor
authors.forename: G N
authors.initials: GN
authors.lastname: Gill
authors.forename: J B
authors.initials: JB
authors.lastname: Thomas
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019281:Dimerization; D004330:Drosophila / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D018398:Homeodomain Proteins; D019476:Insect Proteins / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014921:Wings, Animal / Q000002:abnormalities / Q000254:growth & development
chemical_list: C108740:Chi protein, Drosophila; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D060850:LIM-Homeodomain Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C074459:ap protein, Drosophila