Isolation and localization of an IDDMK1,2-22-related human endogenous retroviral gene, and identification of a CA repeat marker at its locus.
We intended to confirm genetically the involvement of the IDDMK1,2-22 gene in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). For this purpose, we isolated a human endogenous retrovirus gene, possibly corresponding to IDDMK1,2-22. The isolated gene showed 99.8% and 99.7% homologies in nucleotide sequences to a part of the env region and of the 3'-LTR region, respectively, compared to those of IDDMK1,2-22 deposited in GenBank. The gene also showed a close relation to HERV-K18, of which the 3'-LTR sequence gave 99.5% homology. It seemed likely that these genes represented the same single gene. The newly isolated gene was present in the first intron of the CD48 gene and was located on chromosome 1q21.2-q22. A CA repeat marker was found approximately 20 kb upstream from the 5'-end of the 5'-LTR of the gene.
Hasuike
S
S
Department of Obstetrics and Gynecology, Asahikawa Medical College, Japan.
Miura
K
K
Miyoshi
O
O
Miyamoto
T
T
Niikawa
N
N
Jinno
Y
Y
Ishikawa
M
M
D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D004279:DNA, Viral / Q000235:genetics; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D018900:Dinucleotide Repeats; D020077:Endogenous Retroviruses / Q000235:genetics*; D015752:Genes, env; D005819:Genetic Markers; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D005819:Genetic Markers