title: Autocrine induction of substance P mRNA and peptide in cultured normal human keratinocytes.
abstract: In this study, we have demonstrated that normal cultured keratinocytes (KCs) could generate significant endogenous substance P (SP) in a dose- and time-dependent response to exogenous SP by sensitive ELISA assay and express preprotachinin-a mRNA by RT-PCR and Southern blotting. We performed immunohistochemical analysis to confirm the presence of SP in cultured keratinocytes. In contrast, adrenaline, acetylcholine, histamine and CGRP induced only low amount of SP from cultured normal human KCs. This is the first report that SP can be induced by skin epithelial cells in response to exogenous SP and KC derived SP might play an important role in induction and acceleration of certain cutaneous diseases.
authors.affiliation: Department of Dermatology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Bae
authors.forename: Y
authors.initials: Y
authors.lastname: Matsunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: I
authors.initials: I
authors.lastname: Katayama
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D019898:Autocrine Communication; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D005786:Gene Expression Regulation; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology*; D009490:Neurosecretory Systems / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012879:Skin Physiological Phenomena; D013373:Substance P / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D013373:Substance P; D006632:Histamine; C042867:spantide; D003513:Cycloheximide; D015740:Calcitonin Gene-Related Peptide; D000109:Acetylcholine; D004837:Epinephrine