Chemiluminescence Western blot assay for the detection of immunity against parvovirus B19 VP1 and VP2 linear epitopes using a videocamera based luminograph.
A chemiluminescent Western blot (WB) assay was developed to detect the immune status against linear epitopes of parvovirus B19 structural proteins VP1 and VP2. The chemiluminescent WB assay combined the sensitivity of chemiluminescent substrates and the objective evaluation of the luminescent signal obtained with a new system which consists of a videocamera-based, high-performance, low light level imaging luminograph connected to a personal computer for image analysis. The potential for diagnostic purposes was evaluated using reference serum samples and 75 clinical samples from patients with different clinical conditions and laboratory evaluations regarding B19 infection. The chemiluminescent Western blot assay provided reproducible results, an objective evaluation of the results and a semi-quantitative analysis of the presence of antibodies against VP1 and VP2 in human sera. The chemiluminescent Western blot assay proved more sensitive than the classic colourimetric Western blot assay.
Manaresi
E
E
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
Pasini
P
P
Gallinella
G
G
Gentilomi
G
G
Venturoli
S
S
Roda
A
A
Zerbini
M
M
Musiani
M
M
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D015153:Blotting, Western / Q000379:methods*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002648:Child; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D008163:Luminescent Measurements; D008875:Middle Aged; D016732:Parvovirus B19, Human / Q000276:immunology*; D014741:Video Recording
D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19