title: Collagen (Cn-I) binding by gut lactobacilli.
abstract: Four gut Lactobacillus strains displaying the features which make them particularly promising for the preparation of probiotic products were investigated together with 5 fresh isolates and one collection strain of Lactobacillus plantarum for their ability to bind type I collagen (Cn-I). Immobilised Cn-I in microtitre plates was bound only by 3 strains of gut lactobacilli from piglets and the collection strain Lactobacillus plantarum LHI 10 from Prague in range of A570nm readings 0.114-0.221. Six strains (isolates from turkeys and a calf) did not bind Cn-I (A570nm < 0.1) in this assay. An influence of cultivation medium on Cn-I binding was significant (P < 0.001) in all four adherent strains. Significantly higher (P < 0.001) binding of Cn-I was observed for Lactobacillus casei L 81 and Lactobacillus plantarum LHI 10 grown on solid medium (MRS agar) than for MRS broth-grown cells, however, Lactobacillus plantarum L 5 and Lactobacillus fermentum L 435 expressed significantly (P < 0.001) higher Cn-I binding during cultivation in MRS broth. The specificity of the binding was confirmed because the Cn-I binding by lactobacilli after their preincubation with this protein was completely abolished. Three selected inhibitors (fucoidan, heparan sulphate and hyaluronic acid) significantly (P < 0.001) reduced Cn-I binding by the Lactobacillus plantarum L 5 strain. Following up on some earlier strain characteristics, these results suggest that the selected piglets lactobacilli are also able to bind Cn-I and therefore should antagonize collagen niche colonization by various enteropathogens when used for probiotic purposes.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
authors.forename: I
authors.initials: I
authors.lastname: Styriak
authors.forename: V
authors.initials: V
authors.lastname: Demecková
authors.forename: R
authors.initials: R
authors.lastname: Nemcová
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D007082:Ileum; D007413:Intestinal Mucosa / Q000382:microbiology*; D007583:Jejunum; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism; D013552:Swine / Q000382:microbiology*
chemical_list: D003094:Collagen