Molecular cloning, expression, and mapping of a novel human cDNA, GRP17, highly homologous to human gadd45 and murine MyD118.
From a human placenta cDNA library, we isolated a novel gene whose predicted product is highly homologous in amino acid sequence to human gadd45 and murine MyD118 proteins (about 55% and 52% identity, respectively). The cDNA clone, designated GRP17 (gadd-related protein, 17 kDa), contained a 477-bp open reading frame encoding 159 amino acids. Northern blot analysis revealed strong expression of a 1.35-kb transcript in heart, placenta, liver, skeletal muscle, prostate, testis, and ovary. A 1.7-kb additional transcript was detected in liver. We assigned the GRP17 gene to chromosome 9q22.1-q22.2 by fluorescence in situ hybridization (FISH).
Suzuki
M
M
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.
Watanabe
T K
TK
Fujiwara
T
T
Nakamura Yp6
Takahashi
E
E
Tanigami
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004249:DNA Damage; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D000097642:GADD45 Proteins
D000943:Antigens, Differentiation; D002352:Carrier Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011994:Recombinant Proteins; D000097642:GADD45 Proteins; C086470:GADD45B protein, human; C485654:GADD45G protein, human; C490125:Gadd45b protein, mouse