Solution structure of plantaricin C, a novel lantibiotic.
Plantaricin C, a bacteriocin produced by a Lactobacillus plantarum strain of dairy origin, is a lantibiotic. One dehydroalanine, one lanthionine and three beta-methyl-lanthionine residues were found in its 27 amino acid sequence. The plantaricin C structure has two parts: the first comprises the six NH2-terminal residues, four of which are lysines, which confer a strong positive charge to this stretch. The amino acids in positions 7 and 27 form the lanthionine bridge, giving a globular conformation to the rest of the molecule. The beta-methyl-lanthionine bridges are established between residues 12-15, 13-18 and 23-26. This central region has a charge distribution compatible with an amphipathic alpha-helix, through which plantaricin C would become inserted into the membrane matrix of sensitive organisms, provoking the opening of pores and leakage of the cytoplasmic content.
Turner
D L
DL
Department of Chemistry, University of Southampton, UK. dlt@soton.ac.uk
Brennan
L
L
Meyer
H E
HE
Lohaus
C
C
Siethoff
C
C
Costa
H S
HS
Gonzalez
B
B
Santos
H
H
Suárez
J E
JE
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001430:Bacteriocins / Q000737:chemistry*; D007778:Lactobacillus / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010455:Peptides; D011487:Protein Conformation; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055672:Static Electricity
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; D012996:Solutions; C089260:plantaricin C