title: DNA-surfactant interactions: coupled cooperativity in ligand binding leads to duplex stabilization.
abstract: The cooperative nature of interaction of cationic surfactants with short oligonucleotides leading to eventual stabilization of DNA duplexes is demonstrated. At submicellar concentrations and DNA:surfactant charge ratios of 0.2 to 0.8, the association of single chain (CTAB) and double chain (DOTAP) surfactants to oligonucleotides is initiated by electrostatic interaction of cationic ligands with polyanionic DNA that aligns the surfactant molecules on the DNA template. This is followed by binding of new surfactant ligands to the initial complex, driven cooperatively by the hydrophobic forces, leading to in situ formation of surfactant-bound and bare duplexes as separate species. These exhibit independent melting behaviour characterised by double transition in thermal UV profiles, with a higher T(m) for surfactant-DNA complexes. Understanding the cooperative binding of the cationic surfactants to the DNA described here may have implications for rational design of DNA binding drugs and DNA delivery systems.
authors.affiliation: Organic Chemistry Synthesis Division, National Chemical Laboratory, Pune, 411008, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Pattarkine
authors.forename: K N
authors.initials: KN
authors.lastname: Ganesh
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004355:Drug Stability; D066298:In Vitro Techniques; D008024:Ligands; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000378:metabolism*; D013696:Temperature
chemical_list: D008024:Ligands; D013501:Surface-Active Agents; D004247:DNA