The in vitro effects of low molecular weight serum fractions on embryonic Wistar rat (Rattus norvegicus) development.
The in vitro embryo culture technique has been used in many research areas as well as for teratologic and toxicologic tests. Usually, homologous serum is used as a culture medium in these techniques. In this study, the effects of low molecular weight serum fractions on embryonic rat development were tested. Using 30 and 50 kDa Macrosep centrifugal concentrators, the homologous serum was centrifuged for 8 h to separate the low molecular weight serum fractions. The embryos were cultured in the sera which included > 30 kDa, > 50 kDa and < 30 + > 50 kDa serum fractions. Whole rat serum (WRS) was also used for control. After a 48-h culture period, embryonic growth and development were assessed using a morphologic scoring system and the protein content of embryos and yolk sacs. The results showed that the embryonic growth and development during organogenesis significantly decreased in > 30 kDa and > 50 kDa serum fractions when compared to WRS. Addition of a < 30 kDa serum fraction to the > 50 kDa serum fraction improved the embryonic growth, but not to the level seen in embryos grown in WRS. While morphological scores for the embryos grown in WRS, > 30 kDa, > 50 kDa and < 30 + > 50 kDa serum fractions were 57.5 +/- 0.83, 44.53 +/- 1.06, 37.81 +/- 1.9 and 45.14 +/- 1.56, respectively, somite numbers were 26.4 +/- 0.28, 20.47 +/- 0.46, 19.15 +/- 0.58 and 21.78 +/- 0.5, yolk sac diameters were 3.35 +/- 0.06, 2.89 +/- 0.05, 2.61 +/- 0.03 and 2.71 +/- 0.04 mm, crown-rump lengths were 3.03 +/- 0.06, 2.72 +/- 0.04, 2.36 +/- 0.04 and 2.52 +/- 0.04 mm, embryo protein contents were 160.93 +/- 6.88, 119.07 +/- 5.15, 67.23 +/- 3.87 and 98.72 +/- 4.87 micrograms and yolk sac protein contents were 114.87 +/- 5.18, 86.33 +/- 1.92, 62.38 +/- 2.7 and 75.88 +/- 2.87 micrograms, respectively. These results suggest that low molecular weight serum fractions could be very important for normal embryonic development.
Ulger
H
H
Department of Anatomy, University of Erciyes, Kayseri, Turkey. ulger@erciyes.edu.tr
Pratten
M K
MK
D000818:Animals; D001798:Blood Proteins / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008970:Molecular Weight; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D001798:Blood Proteins