title: Stimulation of haematogenous liver metastases by ischaemia-reperfusion in rats.
abstract: OBJECTIVE
  To find out whether hepatic ischaemia-reperfusion stimulates hepatic tumour metastases using a cell line of rat ascitic hepatoma (AH130).
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  University laboratories, Japan.
  
  
  METHODS
  118 male Donryu rats.
  
  
  METHODS
  After laparotomy alone (group 1, n = 35) or laparotomy and 20-minutes ischaemia (group 2, n = 34) or laparotomy and 30-minutes ischaemia (group 3, n = 34) of the median and left hepatic lobes, the animals were given either an intraportal injection of 1 x 10(5) or an intravenous injection of 1 x 10(6) viable AH130 cells.
  
  
  METHODS
  10 days after inoculation of tumour cells the number of nodules on the surface of the right lobe and of the median plus left lobes were separately counted for each liver.
  
  
  RESULTS
  Irrespective of the route of tumour inoculation in group 1, there was no significant difference in the number of tumours/g liver between the right and the median plus left lobes. However, in groups 2 and 3, the number of tumours/g liver in the median plus left lobes was significantly higher than in the right lobe (p < 0.05). Furthermore, in the median plus left lobes, animals who had had 30 minutes of ischaemia had significantly more tumours than those in the other two groups (p < 0.01).
  
  
  CONCLUSIONS
  Hepatic ischaemia-reperfusion may increase the risk of development of haematogenous liver metastases, by stimulating tumour cell-endothelial cell interactions.
authors.affiliation: First Department of Surgery, Kobe University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ku
authors.forename: N
authors.initials: N
authors.lastname: Kusunoki
authors.forename: M
authors.initials: M
authors.lastname: Shiotani
authors.forename: I
authors.initials: I
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Iwasaki
authors.forename: M
authors.initials: M
authors.lastname: Tominaga
authors.forename: T
authors.initials: T
authors.lastname: Kitagawa
authors.forename: T
authors.initials: T
authors.lastname: Fukumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroda
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D007511:Ischemia / Q000503:physiopathology; D008099:Liver / Q000098:blood supply / Q000503:physiopathology; D008114:Liver Neoplasms, Experimental / Q000401:mortality / Q000503:physiopathology / Q000556:secondary*; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D009368:Neoplasm Transplantation; D009360:Neoplastic Cells, Circulating; D011446:Prospective Studies; D051381:Rats; D015427:Reperfusion Injury / Q000150:complications* / Q000503:physiopathology; D013997:Time Factors