[Correlation between hepatocyte cytochrome P450IIE1 expression and oxidation, antioxidation in rat nonalcoholic steatosis model].
OBJECTIVE
To study the correlation between hepatocyte cytochrome P450 II E1(CYP II E1) expression and oxidation, antioxidation in rat nonalcoholic steatosis model.


METHODS
The expression of hepatocyte CYP II E1 antigen in rat steatosis model induced by high fat diet was detected with immnohistochemistry. Meanwhile, MDA, SOD, GSH and VitE contents in the liver were measured by biochemical analysis.


RESULTS
Expression of CYP II E1 in rat nonalcoholic steatosis by high fat diet was prominent in hepatic acinar zone 3, and had a more extensive acinar distribution from zone 3 to zone 2, MDA contents in fatty liver was significantly increased than in normal, SOD, GSH and VitE contents were significantly decreased than in normal. There were significant positive correlation between CYP II E1 and MDA (P < 0.01), and negative correlation between CYP II E1 and SOD, GSH, VitE (P < 0.05, P < 0.05, P < 0.01).


CONCLUSIONS
Hepatocyte CYP II E1 expression in nonalcoholic steatosis is prominent in hepatic acinar zone 3. Its expression resembles alcoholic fatty liver. There is significant correlation between CYP II E1 and lipid peroxidation.
Dai
N
N
Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University.
Zeng
M
M
Li
J
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D005234:Fatty Liver / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D019392:Cytochrome P-450 CYP2E1