Expression, crystallization and preliminary X-ray analysis of the two amino-terminal Ig domains of the neural cell adhesion molecule (NCAM).
The two amino-terminal Ig domains of the rat neural cell adhesion molecule, NCAM, have been expressed in the yeast strain Pichia pastoris. The double domain consisting of 191 amino acids was overexpressed, purified and crystallized. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 47.1, b = 122.5, c = 72.9 A and beta = 98.3 degrees. Assuming there are four double domains per asymmetric unit, V(m) is estimated to be 2.41 A(3) Da(-1) and the solvent content to be 42.3%. A native data set has been collected from a single flash-frozen crystal to 1.85 A resolution.
Kasper
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Department of Medicinal Chemistry, Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark. kasper@medchem.dfh.dk
Rasmussen
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Berezin
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Bock
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Larsen
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D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010843:Pichia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D007136:Immunoglobulins; D019006:Neural Cell Adhesion Molecules; D010446:Peptide Fragments; D011994:Recombinant Proteins