title: Purification, crystallization and preliminary X-ray analysis of catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa.
abstract: The catabolic ornithine carbamoyltransferase (OTCase) from Pseudomonas aeruginosa exhibits allosteric behaviour, with two conformational states of the molecule: an active R form and an inactive T form. The enzyme is a dodecamer with a molecular mass of 455700 Da. Three crystal forms have been obtained. Crystals of allosteric state T are rhombohedral, belonging to the R3 space group, with hexagonal unit-cell parameters a = b = 180.6, c = 122.0 A. They diffract to a resolution of 4.5 A. Two crystal forms for allosteric state R have been obtained, with hexagonal and cubic symmetries. Hexagonal crystals, which diffract to a resolution of 3. 4 A, belong to the space group P6(3) with unit-cell parameters a = b = 140.8, c = 145.6 A. The cubic crystals belong to space group I23, with unit-cell parameter a = 134.32 A and diffract to a resolution better than 2.5 A. In all crystal forms, the dodecamer exhibits a 23 point-group symmetry.
authors.affiliation: Laboratoire de Cristallographie Macromoléculaire, Institut de Biologie Structurale J.-P. Ebel (CEA-CNRS), 41 Rue Jules Horowitz, F-38027 Grenoble CEDEX 1, France.
authors.forename: G
authors.initials: G
authors.lastname: Sainz
authors.forename: J
authors.initials: J
authors.lastname: Vicat
authors.forename: R
authors.initials: R
authors.lastname: Kahn
authors.forename: C
authors.initials: C
authors.lastname: Tricot
authors.forename: V
authors.initials: V
authors.lastname: Stalon
authors.forename: O
authors.initials: O
authors.lastname: Dideberg
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D002384:Catalysis; D003460:Crystallization; D018360:Crystallography, X-Ray; D009954:Ornithine Carbamoyltransferase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D009954:Ornithine Carbamoyltransferase