title: Functional expression of hMYH, a human homolog of the Escherichia coli MutY protein.
abstract: We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885-3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mutY mutant and can remove A from an A. 8-hydroxydeoxyguanine mismatch and to a lesser extent can remove A from an A. G mismatch in vitro.
authors.affiliation: Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Slupska
authors.forename: W M
authors.initials: WM
authors.lastname: Luther
authors.forename: J H
authors.initials: JH
authors.lastname: Chiang
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: J H
authors.initials: JH
authors.lastname: Miller
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D020137:Base Pair Mismatch; D045647:DNA Glycosylases; D004260:DNA Repair; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D016296:Mutagenesis; D009699:N-Glycosyl Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
chemical_list: D003838:Deoxyadenine Nucleotides; D011994:Recombinant Proteins; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase