PCR identification of Pseudomonas aeruginosa and direct detection in clinical samples from cystic fibrosis patients.
This report describes a PCR primer pair that targets the algD GDP mannose gene of Pseudomonas aeruginosa and produces a specific 520-bp PCR product useful for P. aeruginosa identification. This PCR assay was tested with 182 isolates of P. aeruginosa and 20 isolates of other bacterial species, and demonstrated 100% specificity and sensitivity. The test was also able to detect P. aeruginosa directly in clinical samples such as sputum or throat swabs obtained from cystic fibrosis patients. The combination of this primer with a universal bacterial primer, acting as a control to assess DNA quality in the sample, resulted in a robust PCR method that can be used for rapid P. aeruginosa detection.
DA Silva Filho
Luiz Vicente Ferreira
LVF
Levi
Joséa Eduardo
JE
Instituto da Crianca, Hospital das Clinicas, University of Sao Paulo Medical School (FMUSP), Av. Dr EnéAas Carvalho de Aguiar, 647 Cerqueira CéAsar, CEP 05403-900 Sao Paulo SP.
Bento
Christina Naomi Oda
CNO
Laboratory of Virology, Instituto de Medicina Tropical de Sao Paulo, FMUSP.
DA Silva Ramos
Scnia Regina Testa
SRT
Rozov
Tatiana
T
D002237:Carbohydrate Dehydrogenases / Q000235:genetics; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D010614:Pharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000150:complications / Q000175:diagnosis*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology
D004269:DNA, Bacterial; D002237:Carbohydrate Dehydrogenases; C020562:GDPmannose dehydrogenase