Duodenal loading with glucose induces fos expression in rat brain: selective blockade by devazepide.
The role of CCK in mediating neuronal activity in the brain in response to dietary carbohydrate was measured by detecting Fos immunoreactivity in response to duodenal glucose load in rats after administration of the CCK-A receptor antagonist devazepide. In adult, male Sprague-Dawley rats, infusion for 30 min of 545 mg (2.18 kcal) dextrose through a duodenal cannula induced Fos expression in the nucleus of the solitary tract (NTS), area postrema (AP), lateral division of the central nucleus of the amygdala (CeAL), and the external subnucleus of the lateral parabrachial nucleus (LPBE). Devazepide treatment (1 mg/kg) attenuated Fos expression in the NTS and AP by 81 and 78%, respectively, but not in the CeAL or LPBE. These results indicate that central neuronal activation is elicited by dietary glucose in the intestinal lumen and that activation of neurons in the NTS and AP is mediated by CCK-A receptors.
Wang
L
L
Center for Ulcer Research and Education/Digestive Disease Research Center, West Los Angeles Veterans Affairs Medical Center, Department of Medicine and Brain Research Institute, University of California, Los Angeles, CA 90073, USA.
Cardin
S
S
Martínez
V
V
Taché
Y
Y
Lloyd
K C
KC
D000818:Animals; D001921:Brain / Q000502:physiology*; D002766:Cholecystokinin / Q000502:physiology; D020109:Devazepide / Q000494:pharmacology*; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004386:Duodenum / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D016762:Genes, fos; D005947:Glucose / Q000008:administration & dosage / Q000502:physiology*; D006727:Hormone Antagonists / Q000494:pharmacology*; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000502:physiology*
D004040:Dietary Carbohydrates; D006727:Hormone Antagonists; D016760:Proto-Oncogene Proteins c-fos; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin; D005947:Glucose; D020109:Devazepide