Organotypic culture of neuroepithelium attached to olfactory bulb from adult mouse as a tool to study neuronal regeneration after ZnSO4 neuroepithelial trauma.
Chemical destruction of the olfactory mucosa leads to a neuronal regeneration. A new organotypic culture model is perfected to improve the regenerating processes study. Explants of neuroepithelium attached to olfactory bulbs were removed from adult mice and cultured, 12 h after ZnSO4 intranasal application. After 3 days in culture, explants showed a necrosis in the olfactory epithelium and a thinning of the olfactory bulb nervous layer. From the fifth day of culture, and mostly the tenth, new cells showed positive immunoreactivity with the olfactory marker protein (OMP), meaning they were regenerating olfactory neurons. Simultaneously, OMP immunoreactivity increased in the nervous and glomerular layers of the olfactory bulb, indicating epithelio-bulbar reconnection. This organotypic culture model could allow further investigations on the regenerating process kinetic.
Michel
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Laboratoire de Neurosciences, EA 481, Besançon, France. vim00@yahoo.com
Monnier
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Cvetkovic
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Math
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D000367:Age Factors; D000818:Animals; D001252:Astringents; D001557:Benzenesulfonates; D048588:Benzoxazines; D004396:Coloring Agents; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009410:Nerve Degeneration / Q000139:chemically induced / Q000503:physiopathology*; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology*; D009924:Organ Culture Techniques / Q000379:methods*; D010078:Oxazines; D019287:Zinc Sulfate
D001252:Astringents; D001557:Benzenesulfonates; D048588:Benzoxazines; D004396:Coloring Agents; D010078:Oxazines; C028911:cresyl violet; D019287:Zinc Sulfate; C086337:acid fuchsin