title: MAGE-1, GAGE-1/-2 gene expression in FNAB of classic variant of papillary thyroid carcinoma and papillary hyperplasia in nodular goiter.
abstract: Differentiation of the papillary variant of papillary thyroid carcinoma (PTC) from papillary hyperplasia in nodular goiter may be difficult in fine-needle aspiration biopsy (FNAB) by means of morphology alone. To improve cytodiagnostic accuracy the occurrence of MAGE-1, GAGE-1/-2 gene expression was analyzed by means of RT-PCR. The genes investigated are recognized by autologous T lymphocytes and are expressed in carcinomas of various sites e.g. lung, ovary, colon but not in non-neoplastic tissue except testis. Routinely obtained smears with cytologic diagnosis of PTC confirmed by histology (n=20) and diagnosis of nodular goiter (n=10) were investigated. The MAGE-1, GAGE-1/-2 PCR products were found in 6/20 of the carcinomas but in none of the benign lesions. To identify PCR products automatic gene-sequencing in all positive cases was performed. The data indicate that MAGE-1, GAGE-1/-2 gene expression may give additional information to delineate PTC from papillary hyperplasia in FNAB.
authors.affiliation: Department of Cytopathology, Georg-August University Gottingen, 37075 Gottingen, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Ruschenburg
authors.forename: A
authors.initials: A
authors.lastname: Kubitz
authors.forename: T
authors.initials: T
authors.lastname: Schlott
authors.forename: M
authors.initials: M
authors.lastname: Korabiowska
authors.forename: M
authors.initials: M
authors.lastname: Droese
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics; D001707:Biopsy, Needle; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology*; D015870:Gene Expression; D006044:Goiter, Nodular / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D006965:Hyperplasia; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; C095365:GAGE1 protein, human; C095366:GAGE2A protein, human; C072011:MAGEA1 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins