Molecular cloning of EDG-3 and N-Shc genes from the puffer fish, Fugu rubripes, and conservation of synteny with the human genome.
EDG-3 is a receptor for sphingosine-1-phosphate mapped on human chromosome 9q22.1-q22.2. We used the compact Fugu genome for its linkage analysis. The Fugu EDG-3 was composed of one intron and two exons, encoding a 384 amino acid protein that has 56.9% homology with the human EDG-3. Approximately 3 kb apart, a neuronal Shc (N-Shc) gene was identified. It spans 7 kb containing 12 coding exons, and has an overall 53.4% similarity with the human protein. We mapped the human N-Shc gene to chromosome 9q21.3-q22.2. This is the first report of the genomic structure and the linkage of these two genes conserved between Fugu and human.
Yamaguchi
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F
The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, CA 94704, USA. bunchan@molsci.org
Yamaguchi
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K
Tokuda
M
M
Brenner
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005400:Fishes, Poisonous / Q000235:genetics; D008040:Genetic Linkage; D015894:Genome, Human; D006801:Humans; D020672:I-kappa B Proteins; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D009479:Neuropeptides / Q000235:genetics*; D049312:Receptors, Lysophospholipid; D017386:Sequence Homology, Amino Acid; D055765:Shc Signaling Adaptor Proteins; D000071427:Src Homology 2 Domain-Containing, Transforming Protein 3
D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D009479:Neuropeptides; D049312:Receptors, Lysophospholipid; C122029:SHC3 protein, human; D055765:Shc Signaling Adaptor Proteins; D000071427:Src Homology 2 Domain-Containing, Transforming Protein 3; D000072000:NF-KappaB Inhibitor alpha