title: Optimizing culture of Chlamydia pneumoniae by using multiple centrifugations.
abstract: Three methods for the recovery of Chlamydia pneumoniae from spiked nasopharyngeal and blood specimens, including extended culture and additional centrifugations, were compared. Additional centrifugations and a 7-day culture time resulted in a 500- to 5, 000-fold increase in the number of detectable inclusion-forming units.
authors.affiliation: Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jmp5@cdc.gov
authors.forename: J M
authors.initials: JM
authors.lastname: Pruckler
authors.forename: N
authors.initials: N
authors.lastname: Masse
authors.forename: V A
authors.initials: VA
authors.lastname: Stevens
authors.forename: L
authors.initials: L
authors.lastname: Gang
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: E R
authors.initials: ER
authors.lastname: Zell
authors.forename: S F
authors.initials: SF
authors.lastname: Dowell
authors.forename: B S
authors.initials: BS
authors.lastname: Fields
mesh_terms: D002478:Cells, Cultured; D002498:Centrifugation; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology