title: Ablation of inner hair cells by carboplatin alters cells in the medial K(+) flow route and disrupts tectorial membrane.
abstract: The thesis that K(+) effluxing from inner hair cells (IHCs) cycles medially back to endolymph through inner sulcus and interdental cells (IDCs) was tested by comparing control chinchilla cochleas with those in which IHCs were selectively destroyed by carboplatin. By light microscopy inner sulcus cells appeared tall and nearly empty in control ears, but 4 months after the carboplatin treatment many showed vacuolization and shrinkage. Inner pillar cells also consistently developed abnormal vacuoles after carboplatin treatment. Control cochleas exhibited lateral columns and central clusters of IDCs which at their apex possessed expanded presumably hydrated phalanges. Four months after carboplatin, the IDC epithelium enclosed empty looking spaces and the apical phalangeal compartment collapsed into a thin, apparently dehydrated layer. This alteration was accompanied by changes in the tectorial membrane (TM) whereby the membrane's limbal zone thickened progressively to form a tall hollow mound in advanced lesions. The clear spaces in the epithelium and collapse of the phalanges are thought to reflect diminished flow of ions and fluid through IDCs. The accumulation of limbal TM supports the premise that IDCs secrete macromolecules for TM turnover as well as ions and fluid for promoting lateral migration of its precursor constituents. Occurring after ablation of IHCs by carboplatin, the changes in inner pillar, inner sulcus and IDCs and limbal TM can be viewed as a secondary effect of the interrupted ion efflux from IHCs and as further evidence that this effluent follows a medial route.
authors.affiliation: Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425,USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Spicer
authors.forename: R J
authors.initials: RJ
authors.lastname: Salvi
authors.forename: B A
authors.initials: BA
authors.lastname: Schulte
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016190:Carboplatin / Q000494:pharmacology*; D002682:Chinchilla; D003051:Cochlea / Q000166:cytology / Q000187:drug effects; D006199:Hair Cells, Auditory, Inner / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D013680:Tectorial Membrane / Q000502:physiology*; D013997:Time Factors; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D000970:Antineoplastic Agents; D016190:Carboplatin; D011188:Potassium