title: The effect of a sciatic nerve block on the development of inflammation in carrageenan injected rats.
abstract: Neurogenic inflammation may participate in postoperative inflammatory pain. We evaluated, in the rat, the influence of a short and prolonged sciatic nerve block on carrageenan-induced inflammation, the time course of which may be compared to postoperative inflammation. A catheter was placed on the right sciatic nerve and injected with 0.5% bupivacaine with epinephrine (0.2 mL): one injection in the Short Block Group, and four injections performed at 90-min intervals in the Prolonged Block Group. In all groups, the two hind paws were then injected with carrageenan. The development of inflammation was evaluated in both hind paws by measurement of paw circumference (PC) before, and 1, 2, 3, 4, 6, and 24 h after carrageenan injection. Temperature of both hind paws was evaluated at the same time points. The vocalization threshold to paw pressure test (VTPP) of both hind paws was evaluated at 6, 8, 10, 12, and 24 h after carrageenan injection. The left hind paw was used for the Control Group. A Sham Group had a catheter placed on the sciatic nerve and injected with normal saline. Inflammation developed in the Control Group with a maximum increase of PC (32%) and temperature (14%) 4 h after carrageenan injection and a maximal reduction of VTPP (44%) at 6 h, reflecting mechanical allodynia. A similar evolution was observed in the Sham Group. In the Short Block Group, the nerve block did not influence the PC, the paw temperature, or the VTPP when compared with the Control Group. In the Prolonged Block Group, when compared with the Control Group, the increased PC was reduced throughout the 24 h (P < 0.0001). The maximal increase in PC at 4 h was limited to 23%, as compared with the precarrageenan value. This effect on PC did not persist at 24 h. Paw temperature was increased (P = 0.07) throughout the study in the Prolonged Block Group, as compared with the Control Group. The VTPP reduction was still limited in the Prolonged Block Group at 24 h, as compared with the Control Group (P < 0.0001). We conclude that a prolonged sciatic nerve block limits carrageenan-induced increase in PC and, subsequently, mechanical allodynia at 24 h in rats.
  
  
  CONCLUSIONS
  Our study has shown that a prolonged (6 h) but not a short sciatic nerve block (90 min) can limit edema and related pain after carrageenan-induced inflammation in rat.
authors.affiliation: Laboratoire d'Anesthésie Réanimation Chirurgicale, Université Paris Sud, Département d'Anesthésie Réanimation Chirurgicale, Bicêtre, France.
authors.forename: M E
authors.initials: ME
authors.lastname: Gentili
authors.forename: J X
authors.initials: JX
authors.lastname: Mazoit
authors.forename: K
authors.initials: K
authors.lastname: Samii K
authors.forename: D
authors.initials: D
authors.lastname: Fletcher
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage; D000818:Animals; D001831:Body Temperature / Q000502:physiology; D002045:Bupivacaine / Q000008:administration & dosage; D002351:Carrageenan; D004487:Edema / Q000209:etiology; D004837:Epinephrine / Q000008:administration & dosage; D005079:Excipients; D005500:Follow-Up Studies; D006614:Hindlimb; D008297:Male; D009407:Nerve Block / Q000009:adverse effects; D020078:Neurogenic Inflammation / Q000209:etiology*; D017288:Pain Threshold / Q000502:physiology; D010149:Postoperative Pain / Q000209:etiology*; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve; D013997:Time Factors; D014662:Vasoconstrictor Agents / Q000008:administration & dosage
chemical_list: D000779:Anesthetics, Local; D005079:Excipients; D014662:Vasoconstrictor Agents; D002351:Carrageenan; D002045:Bupivacaine; D004837:Epinephrine