Internal ribosome entry site biology and its use in expression vectors.
Internal ribosome entry sites (IRESs) are cis-acting elements that recruit the small ribosomal subunits to an internal initiator codon in the mRNA with the help of cellular trans-acting factors. The recent discovery of the IRES recognition site of the eIF4G initiation factor is beginning to shed some light into how IRES elements are recognized by the translational machinery. Additionally, the progress made in the understanding of the parameters that influence start codon selection will be instrumental in establishing the rational design of bicistronic expression vectors.
Martínez-Salas
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Centro de Biología Molecular Severo Ochoa Consejo Superior de Investigaciones Cientificas Universidad Autónoma de Madrid Cantoblanco, 28049, Madrid, Spain. emartinez@cbm.uam.es
D000818:Animals; D018387:Codon, Initiator; D005822:Genetic Vectors; D006801:Humans; D014176:Protein Biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012270:Ribosomes / Q000235:genetics*; D014780:Viruses / Q000235:genetics
D018387:Codon, Initiator