Increase in blood bradykinin concentration after eccentric weight-training exercise in men.
The purpose of this study was to verify the possible appearance in the blood of bradykinin (BK) and des-Arg(9)-bradykinin (des-Arg(9)-BK) after eccentric exercise in 13 male subjects. Eccentric exercise (5 x 10 leg presses at 120% maximal voluntary concentric contraction) resulted in muscle damage and inflammation, as suggested by the significant increase in serum creatine kinase activity (from 204 +/- 41 to 322 +/- 63 U/l 12 h postexercise) and by severe lasting pain, which also peaked at 12 h postexercise. Blood BK and des-Arg(9)-BK concentrations were measured by competitive enzyme immunoassays using highly specific polyclonal rabbit IgG. Des-Arg(9)-BK concentration was not modified (preexercise: 44 +/- 14 pmol/l; pooled postexercise: 47 +/- 4 pmol/l). In contrast, BK concentration significantly increased immediately after the exercise session (68 +/- 9 vs. 42 +/- 3 pmol/l preexercise) and returned to basal values at 12, 24, and 48 h (pooled value: 40 +/- 4 pmol/l). This observation suggests that the inflammatory process due to eccentric exercise-induced muscle damage could be mediated in part by BK.
Blais
C
C
Faculté de Pharmacie, Université de Montréal, Montreal H3C 3J7, H3C 3P8 Canada.
Adam
A
A
Massicotte
D
D
Péronnet
F
F
D000328:Adult; D001920:Bradykinin / Q000031:analogs & derivatives / Q000097:blood*; D003402:Creatine Kinase / Q000097:blood; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries / Q000378:metabolism; D010809:Physical Fitness / Q000502:physiology*; D014891:Weight Lifting / Q000502:physiology*
C024626:bradykinin, des-Arg(9)-; D003402:Creatine Kinase; D001920:Bradykinin