Biochemical and molecular consequences of ethidium bromide treatment on Drosophila cells.
KC167 Drosophila cells were incubated with low concentrations of ethidium bromide (200 ng/ml), causing changes in mitochondrial DNA (mtDNA) content (2-184% of that of controls). SSCP (single strand conformational polymorphism) analysis of mtDNA indicated that the incubation with ethidium bromide also generated mutations. Compared with controls, there were marked reductions in the activities of respiratory complexes III and IV measured in these cells, and in respiration and ATP synthesis capacities measured in isolated mitochondria. These reductions matched that in mtDNA content. In contrast, no link could be demonstrated between mtDNA content and steady-state concentrations of the transcripts of genes COIII and Cyt b.
Morel
F
F
UMR CNRS 6547, Université Blaise-Pascal-Clermont II, Aubiére, France.
Debise
R
R
Renoux
M
M
Touraille
S
S
Ragno
M
M
Alziari
S
S
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D004272:DNA, Mitochondrial / Q000187:drug effects*; D004330:Drosophila; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D004996:Ethidium; D010084:Oxidation-Reduction; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger; D013379:Substrate Specificity
D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III; D004996:Ethidium