Sequential enhanced tyrosine phosphorylation during progressive malachite green induced malignant transformation of Syrian hamster embryo cells in culture is associated with no change in the activity levels of tyrosine phosphatases.
Malachite green (MG) consisting green crystals with a metallic lustre is extremely soluble in water and is highly cytotoxic to mammalian cells and also acts as liver tumor promoter. In view of its industrial importance and possible exposure to human beings, MG poses a potential environmental health hazard. We have earlier reported that MG induces malignant transformation in Syrian hamster embryo (SHE) cells. Since tyrosine phosphorylation and dephosphorylation reactions are known to play critical roles during normal and abnormal cellular proliferation, in this study we have studied the tyrosine phosphorylation, tyrosine phosphorylated proteins and protein tyrosine phosphatases in malignantly transformed cells and during sequential development of cellular transformation by MG compared to control cells. The present investigation shows that enhanced tyrosine phosphorylation and tyrosine phosphorylated proteins associated with the static levels of tyrosine protein phosphatases may probably contribute to the abnormal cellular proliferation during malignant transformation of SHE cells by MG.
Mahudawala
D M
DM
Cellular Carcinogenesis Laboratory, Tata Memorial Centre, Mumbai, India.
Rao
K V
KV
D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008647:Mesocricetus; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012394:Rosaniline Dyes; D014443:Tyrosine / Q000378:metabolism*
D012394:Rosaniline Dyes; C005095:malachite green; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D017027:Protein Tyrosine Phosphatases