Choline acetyltransferase expression during periods of behavioral activity and across natural sleep-wake states in the basal forebrain.
The present study examined whether the expression of the messenger RNA encoding the protein responsible for acetylcholine synthesis is associated with sleep-wakefulness. Choline acetyltransferase messenger RNA levels were analysed using a semi-quantitative assay in which reverse transcription was coupled to complementary DNA amplification using the polymerase chain reaction. To examine the relationship between steady-state messenger RNA and behavioral activity, rats were killed during the day (4.00 p.m.) or night (4.00 a.m.), and tissue from the vertical and horizontal limbs of the diagonal bands of Broca was analysed. Choline acetyltransferase messenger RNA levels were higher during the day than during the night. The second study examined more closely the association between choline acetyltransferase messenger RNA levels and individual bouts of wakefulness, slow-wave sleep or rapid eye movement sleep. Choline acetyltransferase messenger RNA levels were low during wakefulness, intermediate in slow-wave sleep and high during rapid eye movement sleep. In contrast, protein activity, measured at a projection site of cholinergic neurons of the basal forebrain, was higher during wakefulness than during sleep. These findings suggest that choline acetyltransferase protein and messenger RNA levels exhibit an inverse relationship during sleep and wakefulness. The increased messenger RNA expression during sleep is consistent with a restorative function of sleep.
Greco
M A
MA
West Roxbury VAMC and Harvard Medical School, MA 02132, USA.
McCarley
R W
RW
Shiromani
P J
PJ
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D009474:Neurons / Q000201:enzymology; D016548:Prosencephalon / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012895:Sleep, REM / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology*
D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase