title: Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots.
abstract: A high-performance liquid chromatographic method was developed for electrospray mass spectrometric analysis of ginsenosides in Panax ginseng roots. The analyses were performed on a reversed-phase C18 column using a binary eluent (aqueous 8 mM NH4OAc, buffered to pH 7 with NH4OH-acetonitrile) under gradient conditions. Twenty-five ginsenosides could be separated and detected. The mass spectra obtained provided information on their molecular masses. A MS-MS experiment was undertaken in order to determine the sugar unit sequences and the aglycone moieties.
authors.affiliation: Indena SpA, Laboratori Ricerca e Sviluppo, Settala (Milan), Italy.
authors.forename: N
authors.initials: N
authors.lastname: Fuzzati
authors.forename: B
authors.initials: B
authors.lastname: Gabetta
authors.forename: K
authors.initials: K
authors.lastname: Jayakar
authors.forename: R
authors.initials: R
authors.lastname: Pace
authors.forename: F
authors.initials: F
authors.lastname: Peterlongo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides; D013058:Mass Spectrometry / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal; D012503:Saponins / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D036145:Ginsenosides; D012503:Saponins