Inhibition of influenza virus replication by nitric oxide.
Nitric oxide (NO) has been shown to contribute to the pathogenesis of influenza virus-induced pneumonia in mouse models. Here we show that replication of influenza A and B viruses in Mabin Darby canine kidney cells is severely impaired by the NO donor, S-nitroso-N-acetylpenicillamine. Reduction of productively infected cells and virus production proved to correlate with inhibition of viral RNA synthesis, indicating that NO affects an early step in the replication cycle of influenza viruses.
Rimmelzwaan
G F
GF
National Influenza Center, Department of Virology, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands. rimmelzwaan@viro.fgg.eur.nl
Baars
M M
MM
de Lijster
P
P
Fouchier
R A
RA
Osterhaus
A D
AD
D000818:Animals; D004285:Dogs; D009980:Influenza A virus / Q000502:physiology*; D009981:Influenza B virus / Q000502:physiology*; D051379:Mice; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D026423:S-Nitroso-N-Acetylpenicillamine; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D010396:Penicillamine