Separation of bovine serum albumin and its monoclonal antibody from their immunocomplexes by sodium dodecyl sulfate-capillary gel electrophoresis and its application in capillary electrophoresis-based immunoassay.
A non-competitive immunoassay was performed by sodium dodecyl sulfate-capillary gel electrophoresis with UV detection using bovine serum albumin (BSA) and monoclonal anti-BSA. BSA, anti-BSA and their immunocomplexes were well resolved under non-denaturing conditions. A linear calibration curve was obtained and can be used for the quantification of anti-BSA. The limit of detection of anti-BSA was 0.1 microM under the present conditions. Compared with capillary zone electrophoresis, we believed that this method has the potential to be used as a more general format for performing capillary electrophoresis-based immunoassay of medium- and large-sized analytes.
Ou
J P
JP
Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
Chan
S T
ST
Yeung
W S
WS
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate