title: Inhibition of aldosterone production by testosterone in male rats.
abstract: In vivo and in vitro experiments were designed to assess the effect of testosterone on aldosterone secretion in male rats. Orchidectomized rats were injected subcutaneously with oil or testosterone propionate ([TP] 2 mg/kg) for 7 days. Intact rats were injected with oil only. The results indicate that the plasma aldosterone level was higher in orchidectomized versus intact and TP-replaced rats. In the in vitro study, testosterone caused a marked decrease of aldosterone secretion by zona glomerulosa (ZG) cells, but failed to alter the accumulation of intracellular adenosine 3',5'-cyclic monophosphate (cAMP). Testosterone significantly decreased the corticotropin (ACTH)-stimulated production of aldosterone and accumulation of cAMP in rat ZG cells. The conversion of corticosterone to aldosterone and of 25-OH-cholesterol to pregnenolone, as well as angiotensin II (ANG II)-stimulated production of aldosterone, were decreased by testosterone. These results suggest that testosterone inhibits the basal and ANG II- and ACTH-stimulated release of aldosterone, via inhibition of aldosterone synthase activity and cytochrome P-450 side-chain cleavage (P450scc) activity, and ACTH-stimulated cAMP accumulation in rat ZG cells.
authors.affiliation: Department of Physiology, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China.
authors.forename: M M
authors.initials: MM
authors.lastname: Kau
authors.forename: M J
authors.initials: MJ
authors.lastname: Lo
authors.forename: S W
authors.initials: SW
authors.lastname: Wang
authors.forename: S C
authors.initials: SC
authors.lastname: Tsai
authors.forename: J J
authors.initials: JJ
authors.lastname: Chen
authors.forename: Y C
authors.initials: YC
authors.lastname: Chiao
authors.forename: J Y
authors.initials: JY
authors.lastname: Yeh
authors.forename: H
authors.initials: H
authors.lastname: Lin
authors.forename: A Y
authors.initials: AY
authors.lastname: Shum
authors.forename: V S
authors.initials: VS
authors.lastname: Fang
authors.forename: L T
authors.initials: LT
authors.lastname: Ho
authors.forename: P S
authors.initials: PS
authors.lastname: Wang
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000097:blood*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003900:Desoxycorticosterone / Q000378:metabolism; D008297:Male; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology*; D009919:Orchiectomy; D011282:Pregnenediones / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology*; D015384:Zona Glomerulosa / Q000378:metabolism
chemical_list: D000451:Mineralocorticoid Receptor Antagonists; D011282:Pregnenediones; D000804:Angiotensin II; D013739:Testosterone; D003900:Desoxycorticosterone; D000450:Aldosterone; D011374:Progesterone; D011284:Pregnenolone; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine; D003345:Corticosterone