title: Quantitative analysis of the lobular distribution of S-phase in rat liver following dietary administration of di(2-ethylhexyl)phthalate.
abstract: A simple image-analysis method is described, whereby the distribution of hepatocytes that have entered S-phase, as distinguished by the incorporation of bromodeoxyuridine, can be related to the position of the central and portal veins of the hepatic lobule. Hepatocyte S-phase was induced in the livers of male and female F344 rats by administration of di(2-ethylhexyl)phthalate (DEHP) in the diet for 7 days at 2 dose levels, and these livers were used to develop the procedure. The distributions of the S-phase between control and DEHP-treated livers were compared using statistical techniques. The results showed notable differences in the distribution of S-phase between male and female rats as well as limited dose-related effects.
authors.affiliation: Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, United Kingdom. Tony.Soames@ctl.zeneca.com
authors.forename: A R
authors.initials: AR
authors.lastname: Soames
authors.forename: S
authors.initials: S
authors.lastname: Cliffe
authors.forename: I
authors.initials: I
authors.lastname: Pate
authors.forename: J R
authors.initials: JR
authors.lastname: Foster
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001973:Bromodeoxyuridine / Q000378:metabolism; D003627:Data Interpretation, Statistical; D004032:Diet; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D002149:Energy Intake / Q000187:drug effects; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D016196:S Phase; D012737:Sex Factors
chemical_list: D004051:Diethylhexyl Phthalate; D001973:Bromodeoxyuridine