Effects of growth hormone and pair-feeding on leptin mRNA expression in liver and adipose tissue.
Previous research has reported that elevations in circulating growth hormone (GH) levels in meat-type chickens depresses feed intake (FI) more than 30%. It is known that the product of the obese gene, leptin, functions to regulate FI and energy expenditure. To investigate the effect of GH on leptin gene expression, broiler chickens were infused with recombinant chicken GH. To separate any secondary effects of a GH-induced reduction in FI on leptin expression, groups of birds were pair-fed to an average level of voluntary intake similar to GH-treated birds, but received no GH treatment. GH treatment induced a dose-dependent increase in liver leptin gene expression, as measured by reverse transcriptase-polymerase chain reaction, whereas leptin expression in adipose tissue was unchanged. Conversely, in chickens pair-fed (feed-restricted) there was a decrease in leptin gene expression in both tissues. These results provide evidence of a direct effect of GH on leptin gene expression, which is independent of any effects on intake attributable to GH-treatment, and suggest differential regulation of leptin expression between adipose tissue and liver. The results of these experiments provide the first evidence of a relationship between GH and leptin in domestic birds.
Ashwell
C M
CM
Growth Biology Laboratory, Livestock and Poultry Science Institute, USDA-ARS, Beltsville, MD 20705, USA.
McMurtry
J P
JP
Wang
X H
XH
Zhou
Y
Y
Vasilatos-Younken
R
R
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005215:Fasting; D005247:Feeding Behavior; D019382:Human Growth Hormone / Q000494:pharmacology*; D020738:Leptin / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000378:metabolism
D020738:Leptin; D012333:RNA, Messenger; D019382:Human Growth Hormone