Ubiquitous expression of marker transgenes in mice and rats.
The ability to unambiguously mark a cell's genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo. One approach to this challenge has been the creation of transgenic mice expressing a transgene marker that is easily detectable, with no background staining. Multiple transgenic mouse strains bearing constructs with different combinations of promoter elements and coding sequences have been described, each with its own advantages and limitations. In this report we describe the use of an 800-bp promoter fragment isolated from the beta(geo) integration site in ROSA26 mice to target expression of two marker genes. We demonstrate that the ROSA26 promoter directs ubiquitous expression of human placental alkaline phosphatase and enhanced green fluorescent protein during embryonic and postnatal development in mouse and rat. We further demonstrate the general utility of these transgenes for marking donor cells in transplantation studies.
Kisseberth
W C
WC
School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Brettingen
N T
NT
Lohse
J K
JK
Sandgren
E P
EP
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D016384:Consensus Sequence; D005314:Embryonic and Fetal Development; D005091:Exons; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005819:Genetic Markers; D005838:Genotype; D049452:Green Fluorescent Proteins; D006801:Humans; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D016297:Mutagenesis, Site-Directed; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D022661:RNA, Untranslated; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis
D005819:Genetic Markers; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D008164:Luminescent Proteins; D011506:Proteins; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins