Actin cytoskeleton organization in response to integrin-mediated adhesion.
Cell matrix adhesion regulates actin cytoskeleton organization through distinct steps, from formation of filopodia and lamellipodia in the early phases of cell adhesion to organization of focal adhesions and stress fibers in fully adherent cells. In this review, we follow the events induced by integrin-mediated adhesion, such as activation of GTPases Cdc42 and Rac and their effectors and their role in actin polymerization leading to formation of lamellipodia and filopodia and cell spreading. We also show that actin stress fiber and focal adhesion formation following adhesion requires cooperation between integrin-mediated signaling and additional stimuli, including activation of PKC, Rho GTPases, and PTKs such as p125Fak and Src.
Defilippi
P
P
Dipartimento di Genetica, Biologia e Biochimica, Università di Torino, Torino, Italy. paola.defilippi@unito.it
Olivo
C
C
Venturino
M
M
Dolce
L
L
Silengo
L
L
Tarone
G
G
D016475:3T3 Cells; D000199:Actins / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D003599:Cytoskeleton / Q000737:chemistry*; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D020558:GTP Phosphohydrolases / Q000502:physiology; D007150:Immunohistochemistry; D016023:Integrins / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D010590:Phalloidine; D011493:Protein Kinase C / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt; D011554:Pseudopodia / Q000502:physiology; D020764:cdc42 GTP-Binding Protein / Q000502:physiology*
D000199:Actins; D005353:Fibronectins; D016023:Integrins; D010590:Phalloidine; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D011493:Protein Kinase C; D020558:GTP Phosphohydrolases; D020764:cdc42 GTP-Binding Protein