title: Tissue-specific consequences of the anti-adenoviral immune response: implications for cardiac transplants.
abstract: The immune response to adenoviral vectors can induce inflammation and loss of transgene expression in transfected tissues. This would limit the use of adenovirus-mediated gene transfer in disease states in which long-term gene expression is required. While studying the effect of the anti-adenoviral immune response in transplantation, we found that transgene expression persisted in cardiac isografts transfected with an adenovirus encoding beta-galactosidase. Transfected grafts remained free of inflammation, despite the presence of an immune response to the vector. Thus, adenovirus-mediated gene transfer may have therapeutic value in cardiac transplantation and heart diseases. Furthermore, immunological limitations of adenoviral vectors for gene therapy are not universal for all tissue types.
authors.affiliation: Section of General Surgery, Department of Surgery, University of Michigan School of Medicine, Ann Arbor, Michigan 48109-0654, USA.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chan
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: J R
authors.initials: JR
authors.lastname: Piccotti
authors.forename: M C
authors.initials: MC
authors.lastname: Louie
authors.forename: T A
authors.initials: TA
authors.lastname: Judge
authors.forename: L A
authors.initials: LA
authors.lastname: Turka
authors.forename: E J
authors.initials: EJ
authors.lastname: Eichwald
authors.forename: D K
authors.initials: DK
authors.lastname: Bishop
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000276:immunology*; D016027:Heart Transplantation / Q000009:adverse effects / Q000276:immunology*; D007262:Infusions, Intravenous; D051379:Mice; D008810:Mice, Inbred C57BL; D019076:Transgenes; D014185:Transplantation, Isogeneic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D001616:beta-Galactosidase