Expression of goose parvovirus VP1 capsid protein by a baculovirus expression system and establishment of fluorescent antibody test to diagnose goose parvovirus infection.
The gene encoding VP1 capsid protein of a goose parvovirus was cloned into a baculovirus transfer vector and a recombinant baculovirus was produced. The recombinant virus expressed a protein of 88 kDa corresponding to the molecular weight of VP1 protein and the protein was detected by immunoblotting. By indirect fluorescent antibody (IFA) test, the expressed protein was detected in the nucleus of the insect cells as big granules and electron microscopy also showed several big granules in the nucleus infected with the recombinant virus. The IFA test was developed for screening antibody in Muscovy ducks.
Takehara
K
K
Laboratory of Poultry Diseases, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Nakata
T
T
Takizawa
K
K
Limn
C K
CK
Mutoh
K
K
Nakamura
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D016367:Baculoviridae / Q000235:genetics; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D005455:Fluorescent Antibody Technique; D005777:Geese / Q000821:virology*; D010322:Parvoviridae Infections / Q000175:diagnosis*; D017992:Parvovirus / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins