A recombinant human papillomavirus (HPV) type 16 L1-vaccinia virus murine challenge model demonstrates cell-mediated immunity against HPV virus-like particles.
Human papillomavirus (HPV) virus-like particles (VLP) are emerging as the immunogen of choice for prophylactic vaccines. The inability to infect animals with HPV has prevented the testing of potential vaccines such as these in animal systems. This study describes the development of a recombinant vaccinia virus (VV)-HPV type 16 (HPV-16) VLP challenge model to evaluate the efficacy of the cell-mediated immune response following HPV-16 VLP immunization in mice. Inoculation of BALB/c and C57 BL/6 mice with HPV-16 VLP resulted in HPV VLP-specific T cell proliferative responses characterized by the production of both Th1 and Th2 cytokines, and afforded protection against virus challenge from recombinant VV expressing HPV-16 L1 (VVL1R-16). Protection was demonstrated by a 4.6 log10 reduction in ovarian titres of VVL1R-16 in vaccinated BALB/c mice and a 2.3 log10 reduction in vaccinated C57 BL/6 mice, compared with unvaccinated mice.
Marais
Dianne
D
Department of Medical Microbiology, University of Cape Town, Faculty of Health Sciences, Anzio Road, Observatory 7925, Cape Town, South Africa1.
Passmore
Jo-Ann
JA
Department of Medical Microbiology, University of Cape Town, Faculty of Health Sciences, Anzio Road, Observatory 7925, Cape Town, South Africa1.
Maclean
James
J
Department of Medical Microbiology, University of Cape Town, Faculty of Health Sciences, Anzio Road, Observatory 7925, Cape Town, South Africa1.
Rose
Robert
R
Departments of Medicine and Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA2.
Williamson
Anna-Lise
AL
Department of Medical Microbiology, University of Cape Town, Faculty of Health Sciences, Anzio Road, Observatory 7925, Cape Town, South Africa1.
D000818:Animals; D005260:Female; D006801:Humans; D007114:Immunization; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D027383:Papillomaviridae / Q000276:immunology*; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000276:immunology*
D014614:Vaccines, Synthetic; D014765:Viral Vaccines