Different Ca2+ source for slow AHP in completely adapting and repetitive firing pyramidal neurons.
Intracellular recordings in an in vitro neocortical slice preparation from immature rats were used to investigate the Ca2 source for slow afterhyperpolarization (sAHP) generation in pyramidal neurons that exhibit complete spike frequency adaptation (CA neurons). In pyramidal neurons that maintain repetitive firing for long periods of time (RF neurons), N-, P- and Q-type Ca2+ channels supply Ca2+ for sAHP generation. In CA neurons, the sAHP was reduced by only 50% by the combination of antagonists for these Ca2+ channel types and L-type channels. Ryanodine and dantrolene, blockers of Ca2(+)-induced Ca2+ release, reduced the sAHP by approximately 45% in CA neurons, but caused no reduction of the sAHP in RF neurons. Dantrolene application caused CA neurons to fire throughout a 1s suprathreshold current injection (as do RF neurons).
Pineda
J C
JC
Centro de Investigaciones Regionales, Universidad Autonoma de Yucatan, Merida, Mexico.
Galarraga
E
E
Foehring
R C
RC
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000222:Adaptation, Physiological; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000502:physiology; D020907:Calcium Channels, Q-Type / Q000502:physiology; D003620:Dantrolene / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D008297:Male; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D019579:Neocortex / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D009543:Nifedipine / Q000494:pharmacology; D009924:Organ Culture Techniques; D010455:Peptides / Q000494:pharmacology; D017966:Pyramidal Cells / Q000737:chemistry / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012433:Ryanodine / Q000494:pharmacology; D013268:Stimulation, Chemical; D020867:omega-Agatoxin IVA / Q000494:pharmacology; D020866:omega-Conotoxin GVIA / Q000494:pharmacology; D020960:omega-Conotoxins
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D020907:Calcium Channels, Q-Type; D009125:Muscle Relaxants, Central; D010455:Peptides; D020867:omega-Agatoxin IVA; D020960:omega-Conotoxins; C075851:omega-conotoxin-MVIIC; D012433:Ryanodine; D020866:omega-Conotoxin GVIA; D003620:Dantrolene; D009543:Nifedipine; D002118:Calcium