title: Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
abstract: Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycine residues but no significant changes in spore resistance or core hydration.
authors.affiliation: Department of Biology, Virginia Tech, Blacksburg, Virginia 24061, USA. dpopham@vt.edu
authors.forename: D L
authors.initials: DL
authors.lastname: Popham
authors.forename: J
authors.initials: J
authors.lastname: Meador-Parton
authors.forename: C E
authors.initials: CE
authors.lastname: Costello
authors.forename: P
authors.initials: P
authors.lastname: Setlow
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D003960:Diaminopimelic Acid / Q000032:analysis; D006020:Glycopeptides / Q000737:chemistry; D006602:Hexosyltransferases; D009112:Muramic Acids / Q000032:analysis; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D009154:Mutation; D009238:N-Acetylmuramoyl-L-alanine Amidase; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan / Q000737:chemistry*; D010458:Peptidyl Transferases; D013171:Spores, Bacterial / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D002352:Carrier Proteins; D006020:Glycopeptides; D009112:Muramic Acids; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; C031651:N-acetylmuramic acid; D003960:Diaminopimelic Acid; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; C095875:CwlD protein, Bacillus subtilis; D009238:N-Acetylmuramoyl-L-alanine Amidase