Improved human islet isolation by a tube method for collagenase infusion.
BACKGROUND
Collagenase infusion into the pancreatic duct is an essential step in human islet isolation. We developed a new method for ductal canulation and collagenese infusion.


METHODS
A total of 53 pancreata were divided into two groups: group 1 (n=23), the new tube method, and group 2 (n=30), the standard angiocatheter method. In group 1, a polyethylene tube was inserted into the duct and pushed to the tail. The tail was first expanded, followed by expansion of the body and then the head, by pulling out the tube.


RESULTS
Total islet number and number/g pancreas (mean+/-SE) were significantly higher in group 1 (481,123+/-43,218 and 8,010+/-722) (mean+/-SE) than in group 2 (300,974+/-35,122 and 5,090+/-515, P<0.01). Total islet equivalent number and islet equivalent number per gram pancreas were also significantly higher in group 1 (319,176+/-39,354 and 5,455+/-652) than in group 2 (202,022+/-23,331 and 3,722+/-468, P<0.04). Islet purity and fragmentation showed no differences between the groups.


CONCLUSIONS
The tube method improved islet yields. We recommended this method for human islet isolation.
Arita
S
S
Department of Surgery, UCLA School of Medicine and Veterans Affairs Medical Center/West Los Angeles, California 90073, USA.
Smith
C V
CV
Nagai
T
T
Sakamoto
Y
Y
Ochiai
M
M
Maruyama
M
M
Tanabe
Y
Y
Shelvin
L
L
Mullen
Y
Y
D002404:Catheterization / Q000592:standards*; D017364:Collagenases / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007515:Islets of Langerhans; D010179:Pancreas / Q000187:drug effects; D010183:Pancreatic Ducts; D009927:Tissue and Organ Procurement / Q000379:methods*
D017364:Collagenases