title: Human conjunctiva contains high endothelial venules that express lymphocyte homing receptors.
abstract: Lymphocyte trafficking into tissues has been shown to occur across vessels with specialized endothelial cells called high endothelial venules. High endothelial venules naturally occur in lymph nodes and mucosal lymphoid tissues. The presence of high endothelial venules in the conjunctiva has not been demonstrated previously and the purpose of this study was to determine whether there is such a specialized vasculature to facilitate lymphocyte trafficking in conjunctiva associated lymphoid tissue.Samples of conjunctiva from four different individuals were analysed morphometrically for the presence of high endothelial venules by light and electron microscopy, and by immunohistochemistry using antibodies specific for high endothelial venules and for two adhesion molecules namely, Vascular Adhesion Protein-1 and Mucosal Addressin Cell Adhesion Molecule-1, expressed on high endothelial venules found in other tissues. Blood vessels with anatomical features of high endothelial venules were demonstrated in relation to conjunctival follicles and in the lamina propria. Immunohistochemical studies showed positive staining of vessels with anti-high endothelial venule antibodies, and with antibody against Vascular Adhesion Protein-1 but not with antibody against Mucosal Addressin Cell Adhesion Molecule-1. This study reveals that some of the blood vessels associated with conjunctival follicles and lamina propria have features of high endothelial venules. These vessels may play a significant role in lymphocyte homing to the conjunctiva and Vascular Adhesion Protein-1 (but not Mucosal Addressin Cell Adhesion Molecule-1) is one of the vascular addressin/adhesion molecules.
authors.affiliation: Department of Ophthalmology, Queen's Medical Centre, University Hospital, Nottingham, NG7 2UH, England, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Haynes
authors.forename: P J
authors.initials: PJ
authors.lastname: Tighe
authors.forename: R A
authors.initials: RA
authors.lastname: Scott
authors.forename: H
authors.initials: H
authors.lastname: Singh Dua
mesh_terms: D015815:Cell Adhesion Molecules; D003228:Conjunctiva / Q000378:metabolism* / Q000648:ultrastructure; D004729:Endothelium, Lymphatic / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000378:metabolism; D008854:Microscopy, Electron; D009088:Mucoproteins / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C504265:MADCAM1 protein, human; D009088:Mucoproteins; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1