The E-Box motif, recognized by tissue-specific nuclear factor(s), is important for BMP-4 gene expression in osteogenic cells.
We analyzed the promoter activity of mouse BMP-4 gene in three different types of cell lines and demonstrated that position -786 to -691 of the 5'-flanking region of exon I plays a critical role for regulation of tissue specific expression of mouse BMP-4 gene in cells of an osteogenic lineage. By use of site-directed mutagenesis, we have established that the E-box, CATCTG, located within this 5'-flanking region, is essential for tissue-specific transcriptional activation of mouse BMP-4 gene and demonstrated that an osteogenic lineage-specific novel transcriptional factor(s) recognizes this E-box.
Kawasaki
S
S
Department of Orthopaedic Surgery, Department of 2nd Anatomy, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Ebara
S
S
Nakayama
K
K
Takaoka
K
K
D000818:Animals; D001667:Binding, Competitive; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D019070:Cell Lineage; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014018:Tissue Distribution; D014157:Transcription Factors / Q000378:metabolism*
C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors