title: Experimental study of the protection of ischemic preconditioning to spinal cord ischemia.
abstract: BACKGROUND
  Since the advent of ischemic preconditioning in myocardium, more and more attention has been paid to ischemic preconditioning in the central nervous system (CNS). This study was designed to evaluate the protective effect of ischemic preconditioning on spinal cord ischemia.
  
  
  METHODS
  Interventional neuroradiological techniques were used to induce spinal cord ischemia in a rabbit model. Hydrogen electrode technique was used to determine the regional blood flow of the spinal cord. Catecholamines and their metabolites were measured by high performance liquid chromatography (HPLA). Spinal cord evoked potentials were recorded to show spinal cord neurofunction.
  
  
  RESULTS
  After 5 minutes ischemic preconditioning with 20 minutes reperfusion, the regional spinal cord blood flow (rSCBF) was increased, as may be seen by the slight increase of catecholamine, especially NE. This is in positive proportion to the cAMP and indicates the enhancement of the metabolic activities of the spinal cord. After 30 minutes of irreversible ischemia, the great increase in catecholamine caused vascular spasm, endotheliocyte fissure, multiple hemorrhagic suffusion, and necrosis, which would injure the spinal cord as a result. The slight increase of the rSCBF and the maintenance of the rSCBF after irreversible ischemia may enhance the protection of ischemic preconditioning to the spinal cord neurofunction, which was proved by spinal cord evoked potentials (SCEPs).
  
  
  CONCLUSIONS
  Our study showed that 5 minutes of ischemic preconditioning can increase the rSCBF, enhance the tolerance of the spinal cord to irreversible ischemia, and protect the neurofunction of the spinal cord. The biological mechanism of the protective effect of ischemic preconditioning to spinal cord ischemia should be further studied.
authors.affiliation: Beijing Neurosurgical Institute, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Fan
authors.forename: C C
authors.initials: CC
authors.lastname: Wang
authors.forename: F M
authors.initials: FM
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Cheng
authors.forename: H
authors.initials: H
authors.lastname: Qiao
authors.forename: S L
authors.initials: SL
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: F Y
authors.initials: FY
authors.lastname: Xiang
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D005071:Evoked Potentials; D005260:Female; D019194:Ischemic Preconditioning; D008297:Male; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D013116:Spinal Cord / Q000098:blood supply* / Q000503:physiopathology
chemical_list: D002395:Catecholamines