title: Intersubunit proximity of residues in the RecA protein as shown by engineered disulfide cross-links.
abstract: Mutational studies of regions that make up the oligomeric interface within the RecA protein filament structure have shown that F217 is an important determinant of RecA function and oligomer stability. All substitutions, other than Tyr and Cys, completely inhibit RecA activities and exhibit a substantial decrease in protein filament stability [Skiba, M. C., and Knight, K. L. (1994) J. Biol. Chem. 269, 3823-3828; Logan, K. M., et al. (1997) J. Mol. Biol. 266, 306-316]. Although the RecA crystal structure exhibits no obvious constraints that explain this mutational stringency, the structure does reveal a hydrophobic pocket in the neighboring monomer that may accommodate the F217 side chain. Together with the F217C mutation, we have introduced a series of Cys substitutions within the interacting surface on the neighboring monomer and have tested for disulfide formation under various conditions, e.g., with or without ATP and ssDNA. We show that the location of F217 in the crystal structure is in general agreement with its position in the catalytically active RecA-ATP-DNA complex. Functional studies with the mutant proteins support the idea that ATP-induced movement of the wild-type F217 side chain toward this hydrophobic pocket is important in mediating allosteric changes in the RecA protein structure.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655-0103, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Skiba
authors.forename: K M
authors.initials: KM
authors.lastname: Logan
authors.forename: K L
authors.initials: KL
authors.lastname: Knight
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D004260:DNA Repair; D004220:Disulfides / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008958:Models, Molecular; D016254:Mutagenesis, Insertional; D015202:Protein Engineering; D011938:Rec A Recombinases / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D003545:Cysteine; D004220:Disulfides; D011938:Rec A Recombinases; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein