Mated: a putative peptide transporter of Schizophyllum commune expressed in dikaryons.
A gene that exhibits sexually induced transcript accumulation, mtd1, was identified physically linked to a locus that is also under control of the mating-type genes. Genomic and cDNA sequence analyses were performed to determine a large open reading frame which starts with the amino-acid sequence M-A-T-E-D. The ORF showed similarities to a novel family of peptide transporters with at least 12 putative transmembrane structures. A gene disruption resulted in a growth-phenotype with slow aerial mycelium formation that could be complemented with peptone or uracil added to the growth medium.
Lengeler
K B
KB
Friedrich-Schiller-Universität, Biologisch-Pharmazeutische Fakultät, Institut für Mikrobiologie, Mikrobielle Phytopathologie, Beutenbergstrasse 11, D-07745 Jena, Germany.
Kothe
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology*; D003001:Cloning, Molecular; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D015800:Protozoan Proteins; D012313:RNA / Q000032:analysis; D012567:Schizophyllum / Q000166:cytology* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D015800:Protozoan Proteins; C401886:mtd1 protein, Schizophyllum commune; D012313:RNA