Disulfide-linked propeptides stabilize the structure of zymogen and mature pancreatic serine proteases.
Chymotrypsinogen and proelastase 2 are the only pancreatic proteases with propeptides that remain attached to the active enzyme via a disulfide bridge. It is likely, although not proven, that these propeptides are functionally important in the active enzymes, as well as in the zymogens. A mutant chymotrypsin was constructed to test this hypothesis, but it was demonstrated that the lack of the propeptide had no effect on the catalytic efficiency, substrate specificity, or folding of the protein [Venekei, I., et al. (1996) FEBS Lett. 379, 139-142]. In this paper, we investigate the role of the disulfide-linked propeptide in the conformational stability of chymotrypsin(ogen). We compare the stabilities of the wild-type and mutant proteins (lacking propeptide-enzyme interactions) in their zymogen (chymotrypsinogen) and active (chymotrypsin) forms. The mutants exhibited a substantially increased sensitivity to heat denaturation and guanidine hydrochloride unfolding, and a faster loss of activity at extremes of pH relative to those of their wild-type counterparts. From guanidine hydrochloride denaturation experiments, we determined that covalently linked propeptide provides about 24 kJ/mol of free energy of extra stabilization (DeltaDeltaG). In addition, the mutant chymotrypsinogen lacked the normal resistance to digestion by pepsin. This may also explain (besides keeping the zymogen inactive) the evolutionary conservation of the propeptide-enzyme interactions. Tryptophan fluorescence, circular dichroism, microcalorimetric, and activity measurements suggest that the propeptide of chymotrypsin restricts the relative mobility between the two domains of the molecule. In pancreatic serine proteases, such as trypsin, that lose the propeptide upon activation, this function appears to be accomplished via alternative interdomain contacts.
Kardos
J
J
Department of Biochemistry, Biotechnology Research Group of the Hungarian Academy of Sciences, Eötvös University, Budapest.
Bódi
A
A
Závodszky
P
P
Venekei
I
I
Gráf
L
L
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism; D002919:Chymotrypsinogen / Q000737:chemistry / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D019791:Guanidine; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D010434:Pepsin A / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D051381:Rats; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000378:metabolism
D004220:Disulfides; D004792:Enzyme Precursors; D002919:Chymotrypsinogen; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D010434:Pepsin A; D019791:Guanidine