Tip-link integrity on chick tall hair cell stereocilia following intense sound exposure.
Hair bundle tip links have been implicated in the process of hair cell transduction, and previous studies have shown that acoustic overstimulation or exposure to low calcium can disrupt them. Severed tip links would thus be expected to cause a loss in hair cell function. This study investigates the presence of tip links on chick tall hair cells at three exposure durations and three recovery durations. After 4, 24, or 48 h of exposure, and 24, 96, and 288 h of recovery, the basilar papilla was harvested and prepared for scanning electron microscopy. Photomicrographs of hair bundles from sound-exposed and age-matched control ears were obtained in regions of the papilla adjacent to the 'patch' lesion. The percentage of tip links present on these hair bundles was determined from the photomicrographs. After 4, 24, or 48 h of exposure, an average of 49%, 41.1% and 52% of the observed sensory hairs exhibited links. This was significantly lower than that seen in the control ears (71.2%). There also was a reliable recovery of tip links between 24 and 48 h of exposure. The recovery continued and by 24 h post exposure, tip links were present on 61.3% of the sensory hairs. At subsequent recovery intervals, the mean number of tip links on sound-exposed tall hair cells was statistically the same as seen on control cells. The results indicated a predictable loss in the number of tip links during the exposure and their restoration within a relatively short time after the exposure. This structural damage to the tall hair cell, and its recovery, could account for some of the loss and recovery of function in the auditory periphery of these sound-damaged chicks.
Husbands
J M
JM
Department of Otorhinolaryngology: Head and Neck Surgery, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Steinberg
S A
SA
Kurian
R
R
Saunders
J C
JC
D000818:Animals; D002645:Chickens; D002923:Cilia / Q000502:physiology / Q000648:ultrastructure*; D006198:Hair Cells, Auditory / Q000293:injuries* / Q000502:physiology / Q000648:ultrastructure*; D006317:Hearing Loss, Noise-Induced / Q000473:pathology / Q000503:physiopathology; D008855:Microscopy, Electron, Scanning; D009622:Noise / Q000009:adverse effects; D012038:Regeneration; D013997:Time Factors