Involvement of calcitonin gene-related peptide in the modulation of human myometrial contractility during pregnancy.
Calcitonin gene-related peptide (CGRP) is a potent vasodilator and relaxes smooth muscle of a variety of tissues, but the effects of CGRP on human myometrial contractions and the changes in CGRP receptors (CGRP-Rs) in human myometrium have not been described. We report that CGRP induced dose-dependent relaxation in spontaneously contracting myometrium from pregnant women. This relaxation effect is diminished in myometrium obtained from patients during labor and in the nonpregnant state. CGRP-induced relaxations are inhibited by a CGRP-R antagonist (CGRP(8-37)), a soluble guanylate cyclase inhibitor (LY(83583)), and a nitric oxide synthase inhibitor (L-NAME). Both Western blotting and mRNA analysis showed that CGRP-Rs are present in human myometrium, and that the expression of these receptors is increased during pregnancy and decreased during term labor. Immunofluorescent staining revealed that CGRP-Rs are abundant in the myometrial cells of pregnant women who are not in labor, and are minimal in uterine specimens from women in labor and in the nonpregnant state. We conclude that increased CGRP-Rs in myometrium, and resulting enhanced myometrial sensitivity to CGRP, may play a role in maintaining human myometrium in a quiescent state during pregnancy, and that a decline in the CGRP-Rs at term could contribute to the initiation of labor.
Dong
Y L
YL
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas 77555, USA.
Fang
L
L
Kondapaka
S
S
Gangula
P R
PR
Wimalawansa
S J
SJ
Yallampalli
C
C
D000328:Adult; D000634:Aminoquinolines / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology / Q000502:physiology*; D002585:Cesarean Section; D006152:Cyclic GMP / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D006801:Humans; D007044:Hysterectomy; D009215:Myometrium / Q000187:drug effects / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010446:Peptide Fragments / Q000494:pharmacology; D011247:Pregnancy / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016037:Single-Blind Method; D014590:Uterine Contraction / Q000187:drug effects / Q000502:physiology*
D000634:Aminoquinolines; D004791:Enzyme Inhibitors; D010446:Peptide Fragments; C058636:calcitonin gene-related peptide (8-37); C041715:6-anilino-5,8-quinolinedione; D019001:Nitric Oxide Synthase; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D015740:Calcitonin Gene-Related Peptide; D019331:NG-Nitroarginine Methyl Ester