Development of a polymerase chain reaction to detect Vietnamese isolates of duck virus enteritis.
A polymerase chain reaction (PCR) method for the detection of duck virus enteritis (DVE) virus in tissues of infected and affected ducks, and in cell culture was developed. This required us to obtain specific nucleotide sequence information as we could not find any specific data about the genome of the virus. We found the assay to be highly effective in detecting the virus under experimental conditions and to be easily transferred to laboratories in Vietnam where it is being used in studies on the epidemiology of the disease. We have applied this simple and rapid diagnostic method to the detection of DVE isolates grown in cell culture and tissues from infected birds. The assay was also able to differentiate DVE from other avian herpesviruses, such as Marek's disease, infectious laryngotracheitis virus and goose herpesvirus.
Pritchard
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LI
Australian Animal Health Laboratory, Division of Animal Health, CSIRO, Geelong. Ian.Pritchard@dah.csiro.au
Morrissy
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C
Van Phuc
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K
Daniels
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PW
Westbury
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HA
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D015245:Deoxyribonuclease BamHI / Q000737:chemistry; D004372:Ducks; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004751:Enteritis / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D004947:Esophagus / Q000821:virology; D015723:Gene Library; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D001352:Herpesvirus 1, Gallid / Q000235:genetics / Q000302:isolation & purification*; D008099:Liver / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D014744:Vietnam
D017931:DNA Primers; D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI