title: Occupational asthma in a grain worker due to Lepidoglyphus destructor, assessed by bronchial provocation test and induced sputum.
abstract: BACKGROUND
  Occupational asthma (OA) can be a debilitating disease even when removal from the workplace is achieved. Today, the "gold standard" in the assessment of OA is the bronchial provocation test (BPT). Induced sputum is a non-invasive method of exploring airway inflammation which can provide additional information about such challenges and thus could be applied in OA diagnosis and monitoring.
  
  
  METHODS
  We report the study carried out in a grain worker sensitized to Lepidoglyphus destructor (Ld), who suffered from mild asthma at the workplace. Skin prick test and specific serum IgE were measured. Ld-BPT was performed, and the changes in eosinophil rates, and ECP and tryptase levels in induced sputum were studied 30 min and 18 h after Ld-BPT. We also determined the changes in nonspecific bronchial hyperresponsiveness (NSBH), given as PD20 values. To assess the specificity of the changes, we also carried out sputum induction and methacholine challenge after barley-BPT.
  
  
  RESULTS
  An isolated immediate response was obtained with Ld-BPT, while barley-BPT was negative. Induced sputum showed higher tryptase levels 30 min after Ld-BPT, and higher eosinophil and epithelial cell percentages and ECP levels 18 h after Ld-BPT. There was also a decrease in methacholine PD20 values after Ld-BPT. Those changes were not observed after barley-BPT.
  
  
  CONCLUSIONS
  The study of eosinophilic and mast-cell markers in induced sputum provides additional knowledge about the inflammatory process occurring in the airways, suggesting that the study of induced sputum should be considered in the assessment of OA.
authors.affiliation: Allergy Department, Hospital Universitario Nuestra Señora del Pino, Las Palmas de Gran Canaria, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Castillo
authors.forename: A
authors.initials: A
authors.lastname: Rey
authors.forename: N
authors.initials: N
authors.lastname: Ortega
authors.forename: C
authors.initials: C
authors.lastname: Blanco
authors.forename: T
authors.initials: T
authors.lastname: Carrillo
mesh_terms: D000328:Adult; D000382:Agricultural Workers' Diseases / Q000175:diagnosis* / Q000209:etiology; D000485:Allergens; D000818:Animals; D001249:Asthma / Q000175:diagnosis* / Q000209:etiology; D001985:Bronchial Provocation Tests; D002452:Cell Count; D004804:Eosinophils / Q000166:cytology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007428:Intradermal Tests; D008297:Male; D008925:Mites / Q000276:immunology*; D013183:Sputum / Q000737:chemistry* / Q000166:cytology*
chemical_list: D000485:Allergens; D007073:Immunoglobulin E