Generation and characterization of reassortant influenza A viruses propagated in serum-free cultured MDCK-SF1 cells.
The replacement of embryonated chicken eggs by tissue culture cells for the production of influenza vaccines is likely to take place in the near future. Vaccines have already been produced in Madin Darby Canine Kidney (MDCK) cells (Brands et al, in this issue) and extensively tested in phase III trials in humans (Palache et al, in this issue) and it seems a matter of time before such vaccines will become available. For this reason, the generation of high-growth reassortants of influenza A virus strains in MDCK cells has been examined. Influenza A virus reassortants of the field strains A/Taiwan/1/86, A/Johannesburg/82/96 and A/Shenzhen/227/95 (all H1N1) were generated in serum-free cultured MDCK-SF1 cells by dual infection with A/Hong Kong/2/68 (H3N2), a strain selected for its high-growth phenotype. These reassortant viruses all contained at least the matrix gene of A/Hong Kong/2/68 which apparently correlates with an improvement of the viral yield.
Voeten
J T
JT
National Influenza Centre and Institute of Virology, Erasmus University Rotterdam, The Netherlands.
Claas
E C
EC
Brands
R
R
Palache
A M
AM
van Scharrenburg
G J
GJ
Rimmelzwaan
G F
GF
Osterhaus
A D
AD
D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D016895:Culture Media, Serum-Free; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase