title: Acute stress impairs NK cell adhesion and cytotoxicity through CD2, but not LFA-1.
abstract: Using a nonhuman primate model, we examined the mechanisms by which acute social stress inhibits the ability of NK cells to form conjugates with, and lyse target cells. We examined the expression and role of the primary NK cell adhesion molecules, CD2 and LFA-1, in mediating conjugation to target cells. Acute stress induced a decrease in NK cell expression of CD2 (17+/-3%); and to a lesser degree induced a decrease in expression of LFA-1 (CD11a: 8+/-3%; CD18: 7+/-3%). Antibody blocking studies indicated that anti-LFA-1 significantly inhibited NK cell conjugate formation and cytotoxicity in both control (approximately 40% and approximately 50%, respectively) and stressed (approximately 20% and approximately 45%, respectively) conditions. However, anti-CD2 blocked conjugation and cytotoxicity in the control condition by approximately 50%, but had no capacity to further affect the inhibition of conjugation or cytotoxicity of NK cells induced by acute stress. These data indicate that there are differential effects of acute stress on the expression and function of LFA-1 and CD2, and that the stress-induced inhibition of NK cell adhesion and cytotoxicity is dependent upon modulation of adhesion and/or signalling through CD2.
authors.affiliation: Department of Cell Biology and Physiology, University of Pittsburgh, PA 15261, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Rogers
authors.forename: C S
authors.initials: CS
authors.lastname: Brissette-Storkus
authors.forename: W H
authors.initials: WH
authors.lastname: Chambers
authors.forename: J L
authors.initials: JL
authors.lastname: Cameron
mesh_terms: D000208:Acute Disease; D000818:Animals; D018801:CD2 Antigens / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005434:Flow Cytometry; D006854:Hydrocortisone / Q000097:blood; D007074:Immunoglobulin G / Q000494:pharmacology; D018189:Immunomagnetic Separation; D007694:Killer Cells, Natural / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D008252:Macaca fascicularis; D012919:Social Behavior; D013315:Stress, Psychological / Q000276:immunology*
chemical_list: D018801:CD2 Antigens; D007074:Immunoglobulin G; D016169:Lymphocyte Function-Associated Antigen-1; D006854:Hydrocortisone