Production and secretion of adrenomedullin in cultured human alveolar macrophages.
To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89+/-0.12 fmol/10(5) cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM.
Nakayama
M
M
Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Sendai, Miyagi, Japan.
Takahashi
K
K
Murakami
O
O
Yanai
M
M
Sasaki
H
H
Shirato
K
K
Shibahara
S
S
D053607:Adrenomedullin; D000368:Aged; D015152:Blotting, Northern; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016676:Macrophages, Alveolar / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin