[The development of thalamocortical connections studied by using carbocyanine dyes in the early ontogeny of rats].
We studied the differentiation of neurons and development of their connections in the occipital cortex and thalamic areas of the brain in early ontogenesis of rats: from day 11 of embryogenesis until day 19 of postnatal development. We used the method of staining of brain tissues by carbocyanine dyes after its preliminary fixation in aldehydes. Three carbocyanine dyes were used: DiI, DiO and DiA. We showed the dynamics of structural differentiation of the cortical neurons and lateral geniculate body of the thalamus and the specificity of formation of the axonal pathways between the neocortex and thalamic areas. The results obtained confirmed the hypothesis on ordered spatial-temporal growth of the cortical and thalamic fibers in early embryogenesis and revealed synchronous development of both classes of neurons of the lateral geniculate body. Retrograde and anterograde staining of the nerve cells processes by DiI and DiO showed fine morphological details of their structure. DiI provided for a good staining of the cells until day 19 of postnatal ontogenesis and DiO, until the end of embryogenesis, while DiA was not capable of diffusion in the fixed tissue.
Trukhacheva
A A
AA
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Aleksandrova
M A
MA
D000818:Animals; D000831:Animals, Newborn; D002232:Carbocyanines; D002454:Cell Differentiation; D002540:Cerebral Cortex / Q000196:embryology* / Q000254:growth & development; D004396:Coloring Agents; D005260:Female; D005865:Gestational Age; D008297:Male; D009474:Neurons / Q000166:cytology; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013788:Thalamus / Q000196:embryology* / Q000254:growth & development; D013997:Time Factors
D002232:Carbocyanines; D004396:Coloring Agents