title: Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family.
abstract: A neuropeptide F (NPF) was isolated from the fruit fly, Drosophila mellanogaster, based on a radioimmunoassay for a gut peptide from the corn earworm, Helicoverpa zea. A partial sequence was obtained from the fly peptide, and a genomic sequence coding for NPF was cloned after inverse polymerase chain reaction and shown to exist as a single genomic copy. The encoded, putative prepropeptide can be processed into an amidated NPF with 36 residues that is related to invertebrate NPF's and the neuropeptide Y family of vertebrates. In situ hybridization and immunocytochemistry showed that Drosophila NPF was expressed in the brain and midgut of fly larvae and adults.
authors.affiliation: Department of Entomology, University of Georgia, Athens 30602, USA. mbrown@bugs.ent.uga.edu
authors.forename: M R
authors.initials: MR
authors.lastname: Brown
authors.forename: J W
authors.initials: JW
authors.lastname: Crim
authors.forename: R C
authors.initials: RC
authors.lastname: Arata
authors.forename: H N
authors.initials: HN
authors.lastname: Cai
authors.forename: C
authors.initials: C
authors.lastname: Chun
authors.forename: P
authors.initials: P
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000737:chemistry*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007422:Intestines / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000737:chemistry*; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D029721:Drosophila Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; C400215:neuropeptide F, Drosophila; D004247:DNA