[Genetic effects of mitotic poisons and their modification by heat-shock in strains of Drosophila melanogaster having defective adaptive response].
Teratogenic effect of two mitotic poisons, griseofulvin and colchicine, was confirmed. A similar effect of another antimitotic agent, vinblastin, was demonstrated. The teratogenic effect of these poisons is expressed as a reduction of ommatidia in adult flies when the drug is fed to larvae. The highest frequency of phenocopies was recorded in temperature- and mutagen-sensitive strains. The mutagenic activity of vinblastin and griseofulvin was confirmed by the wing-spot test (somatic mutation and recombination test, SMART) in Drosophila melanogaster. In addition, this test demonstrated mutagenic activity of colchicine. All of the mitotic poisons induced small single spots but did not increase frequency of twin spots mwh/flr. Spot frequency was significantly higher in mutagen-sensitive mutants having defective excision repair. Heat shock (45-min exposure at 37 degrees C) decreased the frequency of phenocopies induced by the mitotic poisons. When third-instar larvae were subjected to heat shock prior to drug administration, the frequency of mutant cell clones was significantly reduced. These results indicate participation of heat-shock proteins in the protection of microtubules in actively proliferating cells of D. melanogaster.
Isaenko
O A
OA
Gertsen Russian State Pedagogical University, St Petersburg, Russia.
Shvartsman
P Ia
PIa
D000014:Abnormalities, Drug-Induced; D000222:Adaptation, Physiological; D000818:Animals; D003078:Colchicine / Q000506:poisoning; D004260:DNA Repair; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D006118:Griseofulvin / Q000506:poisoning; D006358:Hot Temperature; D007814:Larva / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects*; D009153:Mutagens / Q000506:poisoning*; D011995:Recombination, Genetic; D012769:Shock / Q000209:etiology*; D014747:Vinblastine / Q000506:poisoning
D009153:Mutagens; D006118:Griseofulvin; D014747:Vinblastine; D003078:Colchicine