title: Abnormal clones of T cells producing interleukin-5 in idiopathic eosinophilia.
abstract: BACKGROUND
  The cause of persistent eosinophilia and the hypereosinophilic syndrome is unknown. Recent work suggests that in some patients with the hypereosinophilic syndrome, a clone of abnormal T cells produces large amounts of interleukin-5, a cytokine required for the growth and differentiation of eosinophils. We examined T-cell surface markers, rearranged T-cell-receptor genes, and in vitro production of cytokines by T cells from patients with idiopathic eosinophilia.
  
  
  METHODS
  The expression of surface molecules on T cells was measured by flow cytometry. Cytokine expression was measured by enzyme-linked immunosorbent assay, flow cytometry, and immunohistochemical analysis. To identify dominant (clonal) rearrangements of the T-cell receptor within the lymphocyte population, Southern blot analysis (beta chain) and the polymerase chain reaction (gamma chain) were performed according to standard protocols.
  
  
  RESULTS
  Among 60 patients with idiopathic eosinophilia, 16 had circulating T cells with an aberrant immunophenotype. In each of these patients, the abnormal immunophenotype was unique. Evidence of clonal rearrangements of the T-cell receptor was obtained in 8 of the 16 patients. In most instances, the abnormal T cells expressed large amounts of surface proteins associated with T-cell activation (the alpha chain of the interleukin-2 receptor and the HLA-DR antigen). Moreover, the aberrant T cells produced large amounts of interleukin-5 in vitro.
  
  
  CONCLUSIONS
  Clonal populations of abnormal T cells producing interleukin-5 occur in some patients with idiopathic eosinophilia.
authors.affiliation: Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos. hus@siaf.unizh.ch
authors.forename: H U
authors.initials: HU
authors.lastname: Simon
authors.forename: S G
authors.initials: SG
authors.lastname: Plötz
authors.forename: R
authors.initials: R
authors.lastname: Dummer
authors.forename: K
authors.initials: K
authors.lastname: Blaser
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002675:Child, Preschool; D002999:Clone Cells; D004802:Eosinophilia / Q000209:etiology / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000276:immunology*; D016130:Immunophenotyping; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D015848:Interleukin-5