Evaluation of the influence of N-nitrosodimethyloamine (NDMA) on the apoptosis of neutrophils of peripheral blood (PMN) and the expression of the IL-6R membrane receptor--in vitro research.
Evaluation of the influence of N-nitrosodimethyloamine (NDMA) on the apoptosis of neutrophils of peripheral blood (PMN) and the expression of the IL-6R membrane receptor - in vitro research. The aim of the present work was the evaluation of N-nitrosodimethyloamine (NDMA) on the induction of apoptosis in the neutrophils of peripheral blood as well as the evaluation of the surface receptors for IL-6. The isolated neutrophils were incubated for 1 and 3 hours with NDMA of a concentration of 2.5, 5, 7.5, and 10 mg/ml. In the samples incubated for 1 hour a significant, dose- dependent increase of apoptosis in the examined cells was observed. In the cells incubated for 3 hours, the increase of apoptosis was observed only at concentration of NDMA of 2.5 and 5 mg/ml. In case of higher concentration used, probably necrotic processes dominated in the cells. No influence of NDMA on the expression of IL-6R was observed.
Jablonski
J
J
Department of Toxicology, Medical Academy, Bialystok, Poland.
Jablonska
E
E
Moniuszko-Jakoniuk
J
J
Holownia
A
A
D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000166:cytology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis*
D019947:Receptors, Interleukin-6; D004128:Dimethylnitrosamine