title: Inhibition of epidermal growth factor-induced interleukin-1beta-converting enzyme expression reduces proliferation in the pancreatic carcinoma cell line AsPC-1.
abstract: It is suggested that interleukin-1beta-converting enzyme (ICE) and ICE-related proteases play an important role in programmed cell death (apoptosis). We investigated ICE expression in the human pancreatic carcinoma cell line AsPC-1 after stimulation with epidermal growth factor and found a time-dependent expression of active ICE induced by epidermal growth factor. Interestingly, ICE expression does not lead to apoptosis. Cell cycle analyses revealed that acetyl-Tyr-Val-Ala-Asp-chloromethylketone-specific and acetyl-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-T yr-Val-Ala-Asp-aldehyde-specific cell-permeable inhibitors of ICE significantly reduced the proliferation of AsPC-1 cells, which suggested a positive influence of ICE on the proliferation in human pancreatic carcinoma cells.
authors.affiliation: Department of General Surgery, University of Ulm, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schlosser
authors.forename: F
authors.initials: F
authors.lastname: Gansauge
authors.forename: T
authors.initials: T
authors.lastname: Schnelldorfer
authors.forename: M
authors.initials: M
authors.lastname: Ramadani
authors.forename: A
authors.initials: A
authors.lastname: Schwarz
authors.forename: H G
authors.initials: HG
authors.lastname: Beger
authors.forename: S
authors.initials: S
authors.lastname: Gansauge
mesh_terms: D017209:Apoptosis; D020170:Caspase 1 / Q000235:genetics*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D004815:Epidermal Growth Factor; D020170:Caspase 1