title: An autopsy case of malignant mesothelioma with osseous and cartilaginous differentiation: bone morphogenetic protein-2 in mesothelial cells and its tumor.
abstract: An autopsy case of biphasic malignant mesothelioma with osseous and cartilaginous differentiation diffusely involving the peritoneal cavity was confirmed by light microscopic histochemistry, immunohistochemistry, and electron microscopy. A reverse transcription-polymerase chain reaction using specific primers for bone morphogenetic protein-2 (BMP-2) revealed weak positive transcript in normal mesothelial cells and up-regulated expression around bone-forming malignant mesothelioma tissue. However, BMP-2 protein expression was detected only in the marginal zone of bone trabeculae and spindle-shaped mesothelioma cells distributed around bone trabeculae in tumor tissue. The distribution of type IV collagen in tumor tissue was in accordance with the BMP-2 expression. Normal mesothelial cells and tumor cells expressed BMP-2 mRNA, but the BMP-2 protein expression was restricted to the bone-forming area in the malignant mesothelioma.
authors.affiliation: Department of Pathology II, Kansai Medical University, Moriguchi, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kiyozuka
authors.forename: H
authors.initials: H
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Yoshizawa
authors.forename: H
authors.initials: H
authors.lastname: Senzaki
authors.forename: D
authors.initials: D
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Bessho
authors.forename: Y
authors.initials: Y
authors.lastname: Okubo
authors.forename: K
authors.initials: K
authors.lastname: Kusumoto
authors.forename: A
authors.initials: A
authors.lastname: Tsubura
mesh_terms: D000008:Abdominal Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism; D001842:Bone and Bones / Q000473:pathology*; D002356:Cartilage / Q000473:pathology*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D017809:Fatal Outcome; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008654:Mesothelioma / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta