The Drosophila GMII gene encodes a Golgi alpha-mannosidase II.
In this paper we show the organisation of the Drosophila gene encoding a Golgi alpha-mannosidase II. We demonstrate that it encodes a functional homologue of the mouse Golgi alpha-mannosidase II. The Drosophila and mouse cDNA sequences translate into amino acid sequences which show 41% identity and 61% similarity. Expression of the Drosophila GMII sequence in CHOP cells produces an enzyme which has mannosidase activity and is inhibited by swainsonine and by CuSO(4.) In cultured Drosophila cells and in Drosophila embryos, antibodies raised against a C-terminal peptide localise this product mainly to the Golgi apparatus as identified by cryo-immuno electron microscopy studies and by antibodies raised against known mammalian Golgi proteins. We discuss these results in terms of the possible use of dGMII as a Drosophila Golgi marker.
Rabouille
C
C
Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. c.rabouille@ed.ac.uk
Kuntz
D A
DA
Lockyer
A
A
Watson
R
R
Signorelli
T
T
Rose
D R
DR
van den Heuvel
M
M
Roberts
D B
DB
D033965:Adaptor Protein Complex alpha Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D000906:Antibodies; D016466:CHO Cells / Q000502:physiology / Q000648:ultrastructure; D003001:Cloning, Molecular; D006224:Cricetinae; D004330:Drosophila; D004625:Embryo, Nonmammalian / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000502:physiology; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D007438:Introns; D008361:Mannosidases / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D016253:Microscopy, Immunoelectron; D050765:Qa-SNARE Proteins; D017026:Swainsonine / Q000494:pharmacology
D033965:Adaptor Protein Complex alpha Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000906:Antibodies; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D017026:Swainsonine