title: Salmonella Abortusequi strains of equine origin harbor a 95kb plasmid responsible for virulence in mice.
abstract: Most Salmonella choleraesuis subsp. choleraesuis serovar Abortusequi strains of equine origin harbor a 95kb plasmid, pSA95. Results of PCR and Southern blot analysis suggest that pSA95 contains spv genes. A pSA95-cured strain of S. Abortusequi was 48 times less virulent to mice than its parental strain. Virulence was restored by reintroduction of pSA95. These results provide clear evidence that pSA95 confers virulence on S. Abortusequi in mice. This is the first report describing a virulence plasmid of S. Abortusequi.
authors.affiliation: Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. akiba@sat.affrc.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Akiba
authors.forename: T
authors.initials: T
authors.lastname: Sameshima
authors.forename: T
authors.initials: T
authors.lastname: Anzai
authors.forename: R
authors.initials: R
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000662:veterinary; D015139:Blotting, Southern / Q000662:veterinary; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D051379:Mice; D008813:Mice, Inbred ICR; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012475:Salmonella / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D014774:Virulence
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial