The effect of riluzole and mannitol on cerebral oedema after cryogenic injury in the mouse.
A cryogenic lesion was produced under halothane anaesthesia in the mouse by placing a cotton swab soaked in liquid nitrogen onto the surface of the cranium. This provoked an oedematous lesion which developed within the hour after the insult and evolved over the following week. Treatment with mannitol at 3 g/kg i.v. caused a significant 22% reduction in oedema 1 h later, when administered immediately after lesion, but not when administered 23-h post lesion. Likewise riluzole (16 mg/kg, i.v.) significantly reduced oedema by 17% when administered immediately after lesion, or 13% (P < 0.05) when administered 23 h after lesion. Repeated doses (2 x 16 mg/kg, i.p.) of riluzole were also able to reduce oedema significantly (24%, P < 0.05) at 24 h post lesion. Riluzole, in four repeated doses of 8 mg/kg i.p. was also able to reduce lesion surface size by 16% (P < 0.05) 48 h after lesion.
Pratt
J
J
CNS Research, CRVA, Rhône-Poulenc Rorer S.A., Vitry-sur-Seine, France.
Archambaud
C
C
Böhme
G A
GA
Roux
M
M
Rataud
J
J
Imperato
A
A
Stutzmann
J M
JM
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D001929:Brain Edema / Q000188:drug therapy* / Q000473:pathology; D020208:Brain Injury, Chronic / Q000188:drug therapy* / Q000473:pathology; D004234:Diuretics, Osmotic / Q000627:therapeutic use*; D005615:Freezing; D008297:Male; D008353:Mannitol / Q000627:therapeutic use*; D051379:Mice; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D019782:Riluzole / Q000627:therapeutic use*; D014867:Water / Q000378:metabolism
D004234:Diuretics, Osmotic; D018696:Neuroprotective Agents; D014867:Water; D008353:Mannitol; D019782:Riluzole