Protective effect of ozone treatment on the injury associated with hepatic ischemia-reperfusion: antioxidant-prooxidant balance.
The effects of ozone treatment on the injury associated to hepatic ischemia-reperfusion (I/R) was evaluated. Ozone treatment (1 mg/kg daily during 10 days by rectal insufflation) is shown to be protective as it attenuated the increases in transaminases (AST, ALT) and lactate levels observed after I/R. I/R leads to a decrease in endogenous antioxidant (SOD and glutathione) and an increase in reactive oxygen species (H2O2) with respect to the control group. However, ozone treatment results in a preservation (glutathione) or increase (SOD) in antioxidant defense and maintains H2O2 at levels comparable to those in the control group. The present study reports a protective effect of ozone treatment on the injury associated to hepatic I/R. The effectiveness of ozone could be related to its action on endogenous antioxidants and prooxidants balance in favour of antioxidants, thus attenuating oxidative stress.
Peralta
C
C
Department of Medical Bioanalysis, Instituto de Investigaciones Biomédicas de Barcelona, CSIC-IDIBAPS, Spain.
León
O S
OS
Xaus
C
C
Prats
N
N
Jalil
E C
EC
Planell
E S
ES
Puig-Parellada
P
P
Gelpí
E
E
Roselló-Catafau
J
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008297:Male; D009336:Necrosis; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015424:Reperfusion; D015427:Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D000637:Transaminases / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D007773:Lactates; D016877:Oxidants; D017382:Reactive Oxygen Species; D010126:Ozone; D013482:Superoxide Dismutase; D000637:Transaminases; D005978:Glutathione