[35S]S-[5-(4-benzoylphenyl)pentyl]glutathione: a versatile radioligand targeting GS-X pumps with the ability of photoaffinity labeling.
[35S]S-[5-(4-benzoylphenyl)pentyl]glutathione (GIF-0017) as a biochemical probe targeting the ATP-dependent organic anion transporters GS-X pumps was synthesized by the reaction of [35S]glutathione and excess 4-(5-bromo)pentylbenzophenone under alkaline conditions, with the radiochemical yield of 24-33% after HPLC purification. Photolysis of the mixture of [35S]GIF-0017 and plasma membrane vesicles prepared from the MRP1 cDNA-transfected LLC-PK1 cells resulted in radio-labeling of a 180-kDa membrane protein. Immunoprecipitation and western blotting using an anti-MRP1 monoclonal antibody confirmed that the [35S]GIF-0017-labeled protein was the MRPI/GS-X pump.
Furuta
K
K
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Japan.
Hosoya
T
T
Tomokiyo
K
K
Okuda
S
S
Kuniyasu
A
A
Nakayama
H
H
Ishikawa
T
T
Suzuki
M
M
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D018374:LLC-PK1 Cells; D026901:Membrane Transport Proteins; D019920:Photoaffinity Labels; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013552:Swine
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D019920:Photoaffinity Labels; D019275:Radiopharmaceuticals; C122088:S-(5-(4-benzoylphenyl)pentyl)glutathione; D013462:Sulfur Radioisotopes; C086860:glutathione transporter; D005978:Glutathione