Biodegradation of xylene and butyl acetate using an aqueous-silicon oil two-phase system.
A stable microbial population, consisting of seven bacterial strains and three yeast strains, was selected in batch cultures on a mixture of ortho and meta-xylene and butyl acetate as the sole source of carbon and energy. This population can completely degrade up to 10 g/L of a mixture of these xenobiotics (70% xylene and 30% butyl acetate wt/wt) in a two-phase aqueous-silicone oil system (70%/30% vol/vol) within 96 h, while for the usual one-phase system very low growth degradation rates were observed. Further organic solvents were tested and finally, silicon oil was selected as the best organic phase for such a two-phase system. With periodical pH adjustments to 6.0 in fed-batch mode, the culture showed a global degradation rate of 63 mg L-1 h-1.
Gardin
H
H
Département de Génie Chimique, Université de Technologie de Compiègne, France.
Lebeault
J M
JM
Pauss
A
A
D000085:Acetates / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012825:Silicon; D014867:Water; D014992:Xylenes / Q000378:metabolism*; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism
D000085:Acetates; D004785:Environmental Pollutants; D014992:Xylenes; D014867:Water; C006848:butyl acetate; D012825:Silicon