Probiotic preparations dose-dependently increase net production rates of organic acids and decrease that of ammonia by pig cecal bacteria in batch culture.
We tested probiotic preparations containing Bifidobacterium, Enterococcus, or Lactobacillus to see if they affect production of organic acids and ammonia by mixed cecal bacteria. Four preparations (Bibalance, Neorakuton, Yakult Seichoyaku, or Yakult Seichoyaku BL) were digested with HC and pancreatin before adding to batch cultures of 50% pig cecal contents. Concentrations of organic acids and ammonia in the culture were quantified. Two preparations stimulated the net production of short-chain fatty acids (SCFA) during the first 8 hr of incubation. From 8 to 24 hr of incubation, all preparations accelerated the net production of SCFA, succinic and lactic acids, and three preparations slowed the net production of isovaleric acid and ammonia, all dose-dependently. The above results indicate that the preparations tested accelerated the breakdown of hard-to-degrade carbohydrate(s) and, possibly thereby, decreased the breakdown of proteinous materials or increased bacterial cell body synthesis in a mixed culture of cecal bacteria.
Sakata
T
T
Department of Basic Sciences, Ishinomaki Senshu University, Ishinomaki, Japan.
Kojima
T
T
Fujieda
M
M
Miyakozawa
M
M
Takahashi
M
M
Ushida
K
K
D019342:Acetic Acid / Q000378:metabolism; D000136:Acid-Base Equilibrium / Q000187:drug effects*; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001644:Bifidobacterium / Q000187:drug effects*; D020148:Butyric Acid / Q000378:metabolism; D002432:Cecum / Q000382:microbiology*; D016983:Enterococcus / Q000187:drug effects*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D045782:Hemiterpenes; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000187:drug effects*; D010421:Pentanoic Acids / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D011422:Propionates / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism; D013552:Swine
D005232:Fatty Acids, Volatile; D045782:Hemiterpenes; D010421:Pentanoic Acids; D011422:Propionates; D020148:Butyric Acid; C008216:isovaleric acid; D019344:Lactic Acid; D000641:Ammonia; D019802:Succinic Acid; C038780:n-pentanoic acid; C029658:propionic acid; D019342:Acetic Acid