Remarkable thermostability of bioelectrodes based on enzymes immobilized within hydrophobic semi-solid matrices.
An enhanced resistance to thermal denaturation was investigated for enzymes immobilized within hydrophobic semi-solid matrices compared with both free enzymes and polymer-entrapped enzymes. The bioelectrodes based on the immobilization of glucose oxidase, lactate oxidase, alcohol oxidase, polyphenol oxidase, peroxidase and L-amino acid oxidase within a carbon-paste matrix were constructed to examine their thermal stabilitiy at 60 degrees C or 80 degrees C. The rhodium/glucose oxidase-containing carbon-paste electrode was found to offer a remarkable stability when incubated at 60 degrees C over a long period of 4 months, with only a decrease of approx. 15% in activity. The comparative studies suggest that thermal stabilization established by this enzyme-immobilization procedure varies with the enzyme's inherent stability, the incubation temperature and the immobilizing reagent, such as pasting liquid.
Liu
J
J
Department of Chemistry, Nantong Medical College, Nantong, JS 226001, People's Republic of China.
Wang
J
J
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D004156:Catechol Oxidase / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006358:Hot Temperature; D050830:L-Amino Acid Oxidase; D008422:Materials Testing; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D012238:Rhodium / Q000737:chemistry
D004800:Enzymes, Immobilized; D002244:Carbon; D012238:Rhodium; D006899:Mixed Function Oxygenases; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D005949:Glucose Oxidase; D004156:Catechol Oxidase; D006735:Horseradish Peroxidase; C021497:lactate 2-monooxygenase; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase