[Hot start of the polymerase chain reaction using DNA helicase].
A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.
Kaboev
O K
OK
Shevelev
I V
IV
Luchkina
L A
LA
Tret'iakov
A N
AN
Shcherbakova
O G
OG
D004265:DNA Helicases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D016963:Thermus thermophilus / Q000201:enzymology
D004269:DNA, Bacterial; D004265:DNA Helicases