title: Respiratory tract inflammation during the induction of chronic bronchitis in rats: role of C-fibres.
abstract: The hypothesis that chronic stimulation of C-fibres by inhaled irritants contributes to the inflammatory changes that occur during the development of chronic bronchitis was tested. The effect of neonatal capsaicin pretreatment on the development of respiratory tract inflammation was examined in a rat model of chronic bronchitis induced by SO2 exposure. Adult capsaicin- and vehicle-treated rats were exposed to SO2 (250 parts per million (ppm) 5 h x day(-1)) for one day, 2 weeks or 4 weeks. Nasal (NL), airway (AL) and bronchoalveolar (BAL) lavages were performed and the number and types of cells in the lavage fluids measured. SO2-induced changes in ventilation were also measured on day 1 of SO2 exposure and in the 3rd and 5th week of exposure. In the vehicle-treated rats, neutrophils became elevated in NL after just one day of SO2 exposure, in AL after 2 weeks, and in the BAL after 4 weeks. In comparison to vehicle animals, more neutrophils were recovered in the AL of capsaicin-treated rats after one day of SO2 (p=0.012), and in the BAL after 2 or 4 weeks (p=0.004 and p=0.01, respectively). On day 1, SO2 caused a transient increase in tidal volume and a sustained decrease in frequency that was not different in capsaicin- and vehicle-treated rats. With continued exposure, these ventilatory responses to SO2 were attenuated in both groups of rats. These data support the hypothesis that the presence of C-fibres limits or delays the inflammation that occurs during the development of chronic bronchitis induced by SO2 exposure. The protective effect of C-fibres is not the result of ventilatory responses to stimulation of these afferents.
authors.affiliation: Physiology Program, Harvard School of Public Health, Boston, MA 02115, USA.
authors.forename: N C
authors.initials: NC
authors.lastname: Long
authors.forename: J
authors.initials: J
authors.lastname: Abraham
authors.forename: L
authors.initials: L
authors.lastname: Kobzik
authors.forename: E A
authors.initials: EA
authors.lastname: Weller
authors.forename: G G
authors.initials: GG
authors.lastname: Krishna Murthy
authors.forename: S A
authors.initials: SA
authors.lastname: Shore
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001980:Bronchi / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D001991:Bronchitis / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002211:Capsaicin / Q000494:pharmacology; D002452:Cell Count; D002908:Chronic Disease; D005260:Female; D007249:Inflammation; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000473:pathology; D018811:Maternal Exposure / Q000009:adverse effects; D017817:Nasal Lavage Fluid / Q000166:cytology; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012129:Respiratory Function Tests; D013458:Sulfur Dioxide / Q000633:toxicity; D015320:Tachykinins / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D015320:Tachykinins; D013458:Sulfur Dioxide; D002211:Capsaicin