Release of renal dipeptidase from rabbit renal proximal tubules and its inhibition by gentamicin.
Effects of several drugs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released from cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
Kang
B Y
BY
Department of Pharmacy, College of Pharmacy, Chonnam National University, Kwangju, Korea.
We
J S
JS
Choi
K
K
Lee
H B
HB
Han
H J
HJ
Park
H S
HS
D000818:Animals; D015153:Blotting, Western; D004150:Dipeptidases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D005839:Gentamicins / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D013696:Temperature
D005839:Gentamicins; D011480:Protease Inhibitors; D011500:Protein Synthesis Inhibitors; D004150:Dipeptidases