Effect of some phytoestrogens on metabolism of rat adipocytes.
The aim of this experiment was to evaluate the direct effect of genistein, daidzein and zearalenone on basal and hormone-induced lipogenesis and lipolysis in isolated rat adipocytes. In lipogenesis, daidzein and zearalenone were used at concentrations of 0.01, 0.1 and 1 mmol x L(-1) and genistein at concentrations of 0.01, 0.3, 0.6 and 1 mmol x L(-1). In lipolysis, concentrations of tested compounds were 0.01, 0.1 and 1 mmol x L(-1). All tested compounds clearly inhibited basal and insulin (1 nmol x L(-1)) stimulated lipogenesis. Basal lipolysis was particularly enhanced by genistein and daidzein at its higher concentrations. The ability of zearalenone to potentiate of basal lipolysis was less marked. Epinephrine (1 micromol x L(-1))-stimulated lipolysis was inhibited by genistein at 1 mmol x L(-1). At a concentration of 0.1 mmol x L(-1) daidzein also augmented epinephrine-stimulated lipolysis and at its highest concentration exhibited an inhibitory effect, similar to genistein. Zearalenone reduced stimulated lipolysis, particularly at the highest concentration.
Kandulska
K
K
Department of Animal Physiology and Biochemistry, University of Agriculture, Poznan, Poland. kakandu@jay-au.poznan.pl
Nogowski
L
L
Szkudelski
T
T
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D004357:Drug Synergism; D004837:Epinephrine / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D048789:Phytoestrogens; D028321:Plant Preparations; D051381:Rats; D017208:Rats, Wistar; D015025:Zearalenone / Q000494:pharmacology
D004968:Estrogens, Non-Steroidal; D007328:Insulin; D007529:Isoflavones; D008055:Lipids; D048789:Phytoestrogens; D028321:Plant Preparations; D015025:Zearalenone; C004742:daidzein; D019833:Genistein; D004837:Epinephrine