Rapid mass screening method and counseling for the 1555A-->G mitochondrial mutation.
The 1555A-->G point mutation is associated with a susceptibility to aminoglycoside antibiotics, and is of particular interest, as it may cause hearing loss even without aminoglycoside exposure. There may be a considerably large high-risk population in Japan, and to avoid possible side effects in this group, a rapid mass screening system and careful counseling are recommended. We are currently using the mutant allele specific amplification (MASA) method to detect the 1555A-->G mitochondrial mutation and we distribute a warning card to subjects found to bear this mutation.
Usami
S
S
Department of Otorhinolaryngology, Hirosaki University School of Medicine, Japan. usami@hsp.md.shinshu-u.ac.jp
Abe
S
S
Shinkawa
H
H
Inoue
Y
Y
Yamaguchi
T
T
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D005817:Genetic Counseling; D005820:Genetic Testing / Q000379:methods*; D006319:Hearing Loss, Sensorineural / Q000139:chemically induced / Q000235:genetics; D006801:Humans; D007564:Japan; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial