title: Serological evidence of an Australian bovine lentivirus.
abstract: Recombinant 26 kDa capsid (CA) proteins of bovine lentiviruses, bovine immunodeficiency virus (BIV) and Jembrana disease virus (JDV), were expressed in Escherichia coli and utilised as antigens for an enzyme-linked immunosorbent assay (ELISA) and a western immunoblot (WIB) procedure for the detection of antibody in dairy cattle in Western Australia. A total of 690 serum samples, 30 from each of 23 farms, were tested by ELISA with a JDV CA protein antigen, and antibody was detected in 3.8% (p<0.05) of the sera. Nine sera from each farm were also tested by WIB with JDV CA protein antigens and antibody was detected in 15.9% of these samples. All ELISA-positive results were also WIB-positive, and all sera antibody-positive by WIB with JDV CA protein antigens were also antibody-positive by the WIB using recombinant BIV CA antigens. This study showed that recombinant protein antigens can be used for serological tests to detect bovine lentivirus infection in Australia.
authors.affiliation: Division of Veterinary and Biomedical Studies, Murdoch University, WA, Australia. eburkala@murdoch.edu.au
authors.forename: E J
authors.initials: EJ
authors.lastname: Burkala
authors.forename: T M
authors.initials: TM
authors.lastname: Ellis
authors.forename: V
authors.initials: V
authors.lastname: Voigt
authors.forename: G E
authors.initials: GE
authors.lastname: Wilcox
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000096:biosynthesis; D015153:Blotting, Western / Q000662:veterinary; D002213:Capsid / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D016087:Immunodeficiency Virus, Bovine / Q000276:immunology / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D011994:Recombinant Proteins; D016036:Seroepidemiologic Studies; D014904:Western Australia / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins