title: Regulation of EGF signaling by cell polarity in MDCK kidney epithelial cells.
abstract: Although the presence of a dominant basolateral sorting signal ensures that the majority of newly synthesized epidermal growth factor (EGF) receptors are delivered directly to the basolateral surface in polarized epithelial cells, a fraction of the receptors are also delivered to the apical surface. Similar to most basolateral membrane proteins, the EGF receptor has an additional signal(s) that selectively targets molecules lacking a dominant basolateral signal to the apical surface. Although the physiological relevance of signal hierarchy is not known, alternative targeting may occur in different epithelial cell types or during development. The goal of this study, therefore, was to determine the effect of membrane domain location on EGF receptor function, focusing on EGF-induced MAP kinase signaling and DNA synthesis. Whereas ligand responsiveness was restricted to the basolateral domain in Madin-Darby canine kidney (MDCK) cells expressing a normal complement of receptors, apical ligand was effective if apical receptor density was increased by overexpression of an exogenous wild-type human gene. Unexpectedly, cells expressing apically localized, cytoplasmically truncated receptors, which behave as dominant negative mutations in other cell types, were also responsive to apical EGF. The cytoplasmically truncated molecules appear to have at least two effects: first, to increase the local concentration of ligand at the apical cell surface; and second, to facilitate activation of the relatively few native EGF receptors normally located at the apical surface. These results indicate that cell context is a critical determinant of receptor mutant protein phenotype.
authors.affiliation: Department of Physiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Hobert
authors.forename: L A
authors.initials: LA
authors.lastname: Friend
authors.forename: C R
authors.initials: CR
authors.lastname: Carlin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology*; D004285:Dogs; D004815:Epidermal Growth Factor / Q000494:pharmacology* / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology; D066246:ErbB Receptors / Q000235:genetics / Q000502:physiology*; D051380:GRB2 Adaptor Protein; D006801:Humans; D007668:Kidney; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D019000:Phosphotyrosine / Q000032:analysis; D011506:Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D011506:Proteins; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D020928:Mitogen-Activated Protein Kinases