The alignment of a voltage-sensing peptide in dodecylphosphocholine micelles and in oriented lipid bilayers by nuclear magnetic resonance and molecular modeling.
The S4 segments of voltage-gated sodium channels are important parts of the voltage-sensing elements of these proteins. Furthermore, the addition of the isolated S4 polypeptide to planar lipid bilayers results in stepwise increases of ion conductivity. In order to gain insight into the mechanisms of pore formation by amphipathic peptides, the structure and orientation of the S4 segment of the first internal repeat of the rat brain II sodium channel was investigated in the presence of DPC micelles by multidimensional solution NMR spectroscopy and solid-state NMR spectroscopy on oriented phospholipid bilayers. Both the anisotropic chemical shift observed by proton-decoupled (15)N solid-state NMR spectroscopy and the attenuating effects of DOXYL-stearates on TOCSY crosspeak intensities of micelle-associated S4 indicate that the central alpha-helical portion of this peptide is oriented approximately parallel to the membrane surface. Simulated annealing and molecular dynamics calculations of the peptide in a biphasic tetrachloromethane-water environment indicate that the peptide alpha-helix extends over approximately 12 residues. A less regular structure further toward the C-terminus allows for the hydrophobic residues of this part of the peptide to be positioned in the tetrachloromethane environment. The implications for possible pore-forming mechanisms are discussed.
Mattila
K
K
Max-Planck-Institut für-Biochemie, 82152 Martinsried, Germany. Kimmo.Mattila@csc.fi
Kinder
R
R
Bechinger
B
B
D000595:Amino Acid Sequence; D000818:Animals; D016880:Anisotropy; D002251:Carbon Tetrachloride / Q000378:metabolism; D003198:Computer Simulation; D004553:Electric Conductivity; D006859:Hydrogen / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008823:Micelles; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000378:metabolism; D011522:Protons; D051381:Rats; D015222:Sodium Channels / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D013113:Spin Labels; D014867:Water / Q000378:metabolism
D008051:Lipid Bilayers; D008823:Micelles; D010446:Peptide Fragments; D011522:Protons; D015222:Sodium Channels; D012996:Solutions; D013113:Spin Labels; D014867:Water; D010767:Phosphorylcholine; C028810:dodecylphosphocholine; D006859:Hydrogen; D002251:Carbon Tetrachloride