A factor that prevents EDTA-induced cell-growth inhibition: purification of transthyretin from chick embryo brain.
We previously reported the inhibition of cell-growth in Neuro-2A cells, mouse neuroblastoma, by Zn2+ chelation with EDTA. This paper describes the purification of a factor that prevents EDTA-induced cell-growth inhibition from chick embryo brain. The purified factor has a molecular mass of 16 kDa on SDS-polyacrylamide gel electrophoresis under reducing conditions. This factor prevents the cell-growth inhibition in a dose-dependent manner and also binds thyroxine. Analysis of the N-terminal amino acid sequence revealed that 40 residues coincide with the sequence of chicken liver transthyretin.
Sakabe
I
I
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu-shi, Japan.
Paul
S
S
Mitsumoto
T
T
Kadoya
T
T
Shinozawa
T
T
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002642:Chick Embryo; D004492:Edetic Acid / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D008099:Liver / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009447:Neuroblastoma; D011228:Prealbumin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017385:Sequence Homology
D006131:Growth Inhibitors; D011228:Prealbumin; D004492:Edetic Acid