title: Analysis of helminth genetic data: comparative examples with Haemonchus contortus isozymes using exact tests or resampling procedures.
abstract: Population genetic data on helminths are scarce and it is important to fine tune analyses of these data, the interpretation of which is based on between-(Fst) and within-(Fis) population variability. Several computer programs are available and confidence intervals of each index are based on different procedures. These programs (Genetix, Diploid, Genepop) were compared with a classical program (Biosys-1) using a large set of Haemonchus contortus isozyme data. The results were identical for all softwares for large differences when natural populations were studied (Fst > 0.25 and Fis > 0.15 or <-0.15). When small Fst were investigated in laboratory-reared populations, the significance of differences was often related to the software used. As a rule of thumb, it is suggested that at least two out of three software packages (based on different calculation procedures) should give similar results before differences are validated.
authors.affiliation: INRA, Ecologie des parasites, Station de Pathologie aviaire et de Parasitologie, Nouzilly, France.
authors.forename: B
authors.initials: B
authors.lastname: Bentounsi
authors.forename: J
authors.initials: J
authors.lastname: Cabaret
mesh_terms: D000818:Animals; D005828:Genetics, Population; D006189:Haemonchus / Q000201:enzymology* / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics*; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics*; D008957:Models, Genetic; D010733:Phosphoglucomutase / Q000235:genetics*; D015203:Reproducibility of Results; D012986:Software Validation
chemical_list: D007527:Isoenzymes; D008291:Malate Dehydrogenase; D008359:Mannose-6-Phosphate Isomerase; D010733:Phosphoglucomutase