title: Biochemical markers of bone turnover after surgical menopause and hormone replacement therapy.
abstract: The objective of this study was to evaluate the effect of surgical menopause and hormone replacement therapy (HRT) on the new biochemical markers of bone turnover. Fourteen women who had undergone surgical menopause and began HRT 3 months after surgery were recruited for a 1-year study. Results were compared with a control group of 31 healthy premenopausal women of similar age. Serum samples were obtained to determine total alkaline phosphatase, bone alkaline phosphatase, propeptides carboxy- and amino-terminal of type I procollagen (PICP, PINP), osteocalcin, tartrate-resistant acid phosphatase, and carboxy-terminal telopeptides of type I collagen (ICTP and serum CTX). Urine samples were analyzed for hydroxyproline, pyridinoline, deoxypyridinoline, alpha- and beta-carboxy-terminal telopeptides of type I collagen (alpha-CTX and beta-CTX), and amino-terminal telopeptide of type I collagen (NTX). Determinations were performed after 3 months of surgical menopause and after 3 and 9 months of HRT. All biochemical markers increased after menopause, and most of them normalized after 9 months of HRT. Serum PINP showed the highest proportion of increased values after surgery among bone formation markers (62%), as well as the highest mean percent increase (101%). Among bone resorption markers in postmenopausal women, urinary beta-CTX, alpha-CTX, NTX, and serum CTX showed the highest proportion of increased values (100%, 67%, 58%, 58%, respectively) as well as the greatest mean percent increase. They were also the markers with the most marked response to HRT. In conclusion, serum PINP is the most sensitive marker of bone formation, whereas beta-CTX is the most sensitive marker of bone resorption after surgical menopause. In addition, both markers showed the highest response after HRT.
authors.affiliation: Service of Rheumatology, Hospital Clinic, University of Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Peris
authors.forename: L
authors.initials: L
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Monegal
authors.forename: N
authors.initials: N
authors.lastname: Guañabens
authors.forename: M
authors.initials: M
authors.lastname: Durán
authors.forename: F
authors.initials: F
authors.lastname: Pons
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez de Osaba
authors.forename: M
authors.initials: M
authors.lastname: Echevarría
authors.forename: A M
authors.initials: AM
authors.lastname: Ballesta
authors.forename: J
authors.initials: J
authors.lastname: Muñoz-Gómez
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000279:Administration, Cutaneous; D000469:Alkaline Phosphatase / Q000097:blood; D000596:Amino Acids / Q000652:urine; D015415:Biomarkers / Q000032:analysis*; D001846:Bone Development; D001862:Bone Resorption / Q000209:etiology / Q000378:metabolism*; D003094:Collagen / Q000097:blood; D024042:Collagen Type I; D004958:Estradiol / Q000008:administration & dosage / Q000627:therapeutic use*; D015914:Estrogen Replacement Therapy; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine; D007527:Isoenzymes / Q000097:blood; D008594:Menopause, Premature; D008875:Middle Aged; D015675:Osteocalcin / Q000097:blood; D010052:Ovariectomy; D010446:Peptide Fragments / Q000097:blood; D010455:Peptides / Q000097:blood; D011347:Procollagen / Q000097:blood; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D024042:Collagen Type I; D007527:Isoenzymes; D010446:Peptide Fragments; D010455:Peptides; D011347:Procollagen; C052929:collagen type I trimeric cross-linked peptide; C047202:procollagen Type I N-terminal peptide; C042813:procollagen type I carboxy terminal peptide; D015675:Osteocalcin; D004958:Estradiol; C015484:pyridinoline; C036020:deoxypyridinoline; D003094:Collagen; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D006909:Hydroxyproline