The hemagglutinating activities of Pseudomonas aeruginosa lectins PA-IL and PA-IIL exhibit opposite temperature profiles due to different receptor types.
The two Pseudomonas aeruginosa lectins PA-IL and PA-IIL, which are very similar in subunit size, composition and properties, but differ in carbohydrate specificity, were shown to exhibit opposite temperature profiles in hemagglutination tests. The galactophilic PA-IL, which interacts with the erythrocyte I antigen (together with B or P system antigens), resembles Ii system-specific 'cold hemagglutinins' (including antibodies and lectins of animals and plants) in low (4 degrees C) temperature optimum, while the hemagglutination by the fucose- and mannose-binding PA-IIL (like that of antibodies and lectins which do not bind to these antigens) increases on raising the temperature from 4 to 37 degrees C and even to 42 degrees C. The preferential production of both P. aeruginosa lectins at 28 degrees C and their much stronger interaction with enzyme (protease or sialidase)-damaged cells, as well as the lower temperature optimum (4 degrees C) of PA-IL-binding to the host cells, may be associated with the saprophytic rather than parasitic designation of this bacterium.
Gilboa-Garber
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N
Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. garbern@mail.biu.ac.il
Sudakevitz
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D
D018829:Adhesins, Bacterial / Q000276:immunology*; D000328:Adult; D004912:Erythrocytes / Q000276:immunology; D006028:Glycosphingolipids / Q000276:immunology; D006384:Hemagglutination; D006801:Humans; D037102:Lectins / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology; D013696:Temperature
D018829:Adhesins, Bacterial; D006028:Glycosphingolipids; C078965:I-antigen; D037102:Lectins; D011971:Receptors, Immunologic; C038692:adhesin, Pseudomonas