Cloning and characterization of a class II DNA photolyase from Chlamydomonas.
Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas encoding a class II DNA photolyase. The PHR2 protein, when expressed in Escherichia coli, is able to complement a DNA photolyase deficiency. The previously described Chlamydomonas mutant, phr1, which is deficient in nuclear but not chloroplast photolyase activity was shown by RFLP analysis not to be linked to the PHR2 gene. Unlike the recently reported class II DNA photolyase from Arabidopsis, the protein encoded by PHR2 is predicted to contain a chloroplast targeting sequence. This result, together with the RFLP data, suggests that PHR2 encodes the chloroplast targeted DNA photolyase.
Petersen
J L
JL
Biochemistry and Molecular Biology Research Group, University of South Dakota, Vermillion 57069-2390, USA.
Lang
D W
DW
Small
G D
GD
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D002696:Chlamydomonas / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes / Q000235:genetics; D005816:Genetic Complementation Test; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D007527:Isoenzymes; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase