Factors affecting survival of cryopreserved oyster (Crassostrea gigas) embryos
A conventional two-step freezing procedure was developed and optimized in order to cryopreserve oyster embryos. The effects of cooling rate, choice of cryoprotectant, and seeding temperature on the survival of late-stage oyster embryos were examined. When these factors were optimized, improved survival rates of 78 +/- 8 and 83 +/- 7% were achieved using 2 M Me(2)SO or glycerol, respectively, as the cryoprotectant. The experimental results indicate that oyster embryos survive after freezing over a broad range of cooling rates ranging from -0.5 to -16 degrees C/min. Me(2)SO, glycerol, propylene glycol, and ethylene glycol may be used as cryoprotectants for the cryopreservation of oyster embryos. Copyright 1999 Academic Press.
Lin
TT
Department of Agricultural Machinery Engineering, National Taiwan University, Taipei, Taiwan, Republic of China.
Chao
NH
Tung
HT