A recessive deletion in the GlcNAc-1-phosphotransferase gene results in peri-implantation embryonic lethality.
Formation of the dolichol oligosaccharide precursor is essential for the production of asparagine- (N-) linked oligosaccharides (N-glycans) in eukaryotic cells. The first step in precursor biosynthesis requires the enzyme UDP-GlcNAc: dolichol phosphate N-acetylglucosamine-1-phosphate transferase (GPT). Without GPT activity, subsequent steps necessary in constructing the oligosaccharide precursor cannot occur. Inhibition of this biosynthetic step using tunicamycin, a GlcNAc analog, produces a deficiency in N-glycosylation in cell lines and embryonic lethality during preimplantation development in vitro, suggesting that N-glycan formation is essential in early embryogenesis. In exploring structure-function relationships among N-glycans, and since tunicamycin has various reported biochemical activities; we have generated a germline deletion in the mouse GPT gene. GPT mutant embryos were analyzed and the phenotypes obtained were compared with previous studies using tunicamycin. We find that embryos homozygous for a deletion in the GPT gene complete preimplantation development and also implant in the uterine epithelium, but die shortly thereafter between days 4-5 postfertilization with cell degeneration apparent among both embryonic and extraembryonic cell types. Of cells derived from these early embryos, neither trophoblast nor embryonic endodermal lineages are able to survive in culture in vitro. These results indicate that GPT function is essential in early embryogenesis and suggest that N-glycosylation is needed for the viability of cells comprising the peri-implantation stage embryo.
Marek
K W
KW
Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.
Vijay
I K
IK
Marth
J D
JD
D000818:Animals; D001755:Blastocyst / Q000473:pathology; D019070:Cell Lineage; D010064:Embryo Implantation; D005314:Embryonic and Fetal Development / Q000235:genetics; D004790:Enzyme Induction; D005260:Female; D005326:Fetal Proteins / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D017353:Gene Deletion; D005804:Genes, Lethal; D005808:Genes, Recessive; D006031:Glycosylation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D013329:Structure-Activity Relationship; D017855:Transferases (Other Substituted Phosphate Groups) / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D014415:Tunicamycin / Q000494:pharmacology
D005326:Fetal Proteins; D009844:Oligosaccharides; D011134:Polysaccharides; D012333:RNA, Messenger; D014415:Tunicamycin; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase