Interactions between social stress and morphine in the periaqueductal gray: effects on affective vocal and reflexive pain responses in rats.
BACKGROUND
Endogenous opioid systems within the mesencephalic periaqueductal gray matter (PAG) appear to be intricately involved in many affective, defensive, submissive, and reflexive responses, and these systems are activated by aversive stimuli.


OBJECTIVE
The present experiments evaluated the influence of opioid receptors within the PAG on affective vocal and reflexive responses to aversive stimuli in socially inexperienced, as well as defensive and submissive responses in defeated, adult male Long-Evans rats.


METHODS
Defeat stress consisted of: (1) an aggressive confrontation with a "resident" stimulus rat in which the experimental "intruder" rat exhibited escape, defensive and submissive behaviors [i.e. upright, supine postures and ultrasonic vocalizations (USV)], and subsequently, (2) protection from the resident rat with a wire mesh screen for ca. 25 min. Defeat stress was immediately followed by an experimental session with thermal antinociceptive and tactile startle stimuli (20 psi airpuffs).


RESULTS
The mu opioid receptor agonist morphine (0.3, 1, 3 microgram IC) attenuated startle-induced USV and the tail-flick reflex in socially inexperienced and defeated rats, with both groups of rats demonstrating equal sensitivity to morphine. Morphine decreased defeat-induced USV and increased the display of the crouch posture in defeated rats; these morphine effects in socially inexperienced and defeated rats were re- versed with the opioid receptor antagonist naltrexone (0.1 mg/kg IP).


CONCLUSIONS
These results reveal that the ventrolateral PAG is an important site in which mu opioid receptor agonists such as morphine mediate affective vocal and submissive responses, yet this structure is not critical in the display of defeat stress-augmented effects of morphine. Endogenous opioid mechanisms appear to participate in the organization of defensive behavior, namely, to facilitate a shift from active to passive forms of coping.
Vivian
J A
JA
Department of Psychology, Tufts University, Medford, Massachusetts, USA.
Miczek
K A
KA
D000374:Aggression / Q000187:drug effects / Q000523:psychology; D000818:Animals; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects; D009294:Narcotics / Q000494:pharmacology*; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement / Q000187:drug effects; D010487:Periaqueductal Gray / Q000187:drug effects*; D010812:Physical Stimulation; D051381:Rats; D020318:Rats, Long-Evans; D017450:Receptors, Opioid, mu / Q000187:drug effects; D012018:Reflex / Q000187:drug effects; D013216:Reflex, Startle / Q000187:drug effects; D012931:Social Environment; D013315:Stress, Psychological / Q000523:psychology*; D013637:Tape Recording; D014743:Videotape Recording; D014828:Vocalization, Animal / Q000187:drug effects*
D009294:Narcotics; D017450:Receptors, Opioid, mu; D009020:Morphine