title: Repair of an osseous facial critical-size defect using augmented fibrin sealant.
abstract: OBJECTIVE
  Osseous defects of the head and neck are a common challenge for the otolaryngologist. To develop improved reconstructive options, osteoconductive engineering experiments are being conducted. A nasal critical-size defect (CSD) model has previously been described in which less than 7% bone healing is observed over 6 months. An implant containing fibrin sealant with and without osteoprogenitor cells is evaluated in this model.
  
  
  METHODS
  Randomized controlled trial using a rodent model.
  
  
  METHODS
  A nasal CSD was surgically created in 18 male retired breeder Sprague-Dawley rats. Six animals were not implanted with any material, six received fibrin sealant consisting of fibrin (25 mg/mL) and thrombin (1000 U/mL), and six were implanted with fibrin sealant and rat calvarial osteoprogenitor cells (1.8 x 10(6) cells/mL). Thirty days later, the animals were examined at necropsy by planimetry, histological analysis of new bone growth, and radiodensitometric analysis of bone thickness.
  
  
  RESULTS
  A thin layer of bone covered the defect in all of the treated animals. A statistically significant increase in bone density (P < .05) between fibrin sealant plus osteoprogenitor cells and each of the other groups was shown using radiodensitometric analysis. Histological analysis also confirmed this difference.
  
  
  CONCLUSIONS
  Osteoprogenitor cells contained within fibrin sealant result in a greater augmentation of bone regeneration than controls or fibrin sealant alone.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, University of Virginia Health Sciences Center, Charlottesville, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Tholpady
authors.forename: R
authors.initials: R
authors.lastname: Schlosser
authors.forename: W
authors.initials: W
authors.lastname: Spotnitz
authors.forename: R C
authors.initials: RC
authors.lastname: Ogle
authors.forename: W H
authors.initials: WH
authors.lastname: Lindsey
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D003720:Densitometry; D005069:Evaluation Studies as Topic; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D008297:Male; D019736:Prostheses and Implants; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014014:Tissue Adhesives / Q000627:therapeutic use*
chemical_list: D018786:Bone Substitutes; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives