The BldD protein from Streptomyces coelicolor is a DNA-binding protein.
Gel mobility shift assays with His-tagged BldD isolated from Escherichia coli have illustrated that BldD is capable of specifically recognizing its own promoter region. DNase I and hydroxyl radical footprinting assays have served to delimit the BldD binding site, revealing that BldD recognizes and binds to a site just upstream from, and overlapping with, the -10 region of the promoter. How BldD binds to its promoter and the effect this binding has on the expression of BldD are discussed.
Elliot
M A
MA
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
Leskiw
B K
BK
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors
D001426:Bacterial Proteins; C111306:BldD protein, Streptomyces coelicolor; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors