The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries.
Metalloproteinase-disintegrins (ADAMs) are membrane-spanning multi-domain proteins containing a zinc metalloproteinase domain and a disintegrin domain which may serve as an integrin ligand. Based on a conserved sequence within the disintegrin domain, GE(E/Q)CDCG, seven genes were isolated from a human genomic library. Two of these genes lack introns and show testis-specific expression (ADAM20 and ADAM21), while the other two genes contain introns (ADAM22 and ADAM23) and are expressed predominantly in the brain. In addition, three pseudogenes were isolated; one of which evolved from ADAM21. Human chromosomal mapping indicated that ADAM22 and ADAM23 mapped to chromosome 7q21 and 2q33, respectively, while the three pseudogenes 1-2, 3-3, and 1-32 mapped to chromosome 14q24.1, 8p23, and 14q24.1, respectively. An ancestral analysis of all known ADAMs indicates that the zinc-binding motif in the catalytic domain arose once in a common ancestor and was lost by those members lacking this motif.
Poindexter
K
K
Immunex Corporation, Seattle, WA 98101, USA.
Nelson
N
N
DuBose
R F
RF
Black
R A
RA
Cerretti
D P
DP
D051722:ADAM Proteins; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002877:Chromosomes, Human; D017124:Conserved Sequence; D019483:Disintegrins / Q000235:genetics*; D019143:Evolution, Molecular; D015698:Genomic Library; D006801:Humans; D007438:Introns; D008565:Membrane Proteins; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics; D010802:Phylogeny; D011544:Pseudogenes; D017386:Sequence Homology, Amino Acid
D019483:Disintegrins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009841:Oligonucleotides; D051722:ADAM Proteins; C110998:ADAM20 protein, human; C110999:ADAM21 protein, human; C498474:ADAM22 protein, human; C121259:Adam23 protein, mouse; D008666:Metalloendopeptidases