Rapid and systemic accumulation of chloroplast mRNA-binding protein transcripts after flame stimulus in tomato.
It has been shown that tomato (Lycopersicon esculentum) plants respond to flame wounding and electrical stimulation by a rapid (15 min) and systemic up-regulation of proteinase inhibitor (pin) genes. To find other genes having a similar expression pattern, we used subtractive cDNA screening between flamed and control plants to select clones up-regulated by flame wounding. We report the characterization of one of them, a chloroplast mRNA-binding protein encoded by a single gene and expressed preferentially in the leaves. Systemic gene expression in response to flaming in the youngest terminal leaf exhibited three distinct phases: a rapid and transient increase (5-15 min) in transcript accumulation, a decline to basal levels (15-45 min), and then a second, more prolonged increase (60-90 min). In contrast, after a mechanical wound the rapid, transient increase (5 min) was followed by a rapid decline to basal levels but no later, prolonged accumulation. In the petiole, the initial flame-wound-evoked transient increase (15 min) was followed by a continuous decline for 3 h. The nature of the wound signal(s) causing such rapid changes in transcript abundance is discussed in relation to electrical signaling, which has recently been implicated in plant responses to wounding.
Vian
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Department of Botany, Box 7612, North Carolina State University, Raleigh, North Carolina 27695-7612, USA. vian@cicsun.univ-bpclermont.fr
Henry-Vian
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C
Davies
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D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000378:metabolism*; D004558:Electric Stimulation; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D007700:Kinetics; D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D018515:Plant Leaves; D010940:Plant Proteins; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018724:Spinacia oleracea / Q000235:genetics / Q000378:metabolism; D060225:Tetraspanin 28; D013997:Time Factors; D014158:Transcription, Genetic
D010940:Plant Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D060225:Tetraspanin 28