title: Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity.
abstract: Sonic hedgehog (Shh) is expressed in the posterior vertebrate limb bud mesenchyme and directs anteroposterior patterning and growth during limb development. Here we report an analysis of the pectoral fin phenotype of zebrafish sonic you mutants, which disrupt the shh gene. We show that Shh is required for the establishment of some aspects of anteroposterior polarity, while other aspects of anteroposterior polarity are established independently of Shh, and only later come to depend on Shh for their maintenance. We also demonstrate that Shh is required for the activation of posterior HoxD genes by retinoic acid. Finally, we show that Shh is required for normal development of the apical ectodermal fold, for growth of the fin bud, and for formation of the fin endoskeleton.
authors.affiliation: Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/III, D-72076 Tübingen, Germany. carl.neumann@tuebingen.mpg.de.
authors.forename: C J
authors.initials: CJ
authors.lastname: Neumann
authors.forename: H
authors.initials: H
authors.lastname: Grandel
authors.forename: W
authors.initials: W
authors.lastname: Gaffield
authors.forename: S
authors.initials: S
authors.lastname: Schulte-Merker
authors.forename: C
authors.initials: C
authors.lastname: Nüsslein-Volhard
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004475:Ectoderm; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics; D007814:Larva; D018878:Limb Buds / Q000166:cytology / Q000254:growth & development*; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D000072079:Patched Receptors; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta; D014212:Tretinoin / Q000494:pharmacology; D014704:Veratrum Alkaloids / Q000494:pharmacology; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004268:DNA-Binding Proteins; D053823:Hedgehog Proteins; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C490944:Hoxd10 protein, mouse; C490939:Hoxd11 protein, mouse; C490943:Hoxd12 protein, mouse; C489520:Hoxd4 protein, mouse; D008565:Membrane Proteins; D000072079:Patched Receptors; D011506:Proteins; D011956:Receptors, Cell Surface; D015534:Trans-Activators; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D014704:Veratrum Alkaloids; D029961:Zebrafish Proteins; C099223:hoxd10a protein, zebrafish; C099221:hoxd11a protein, zebrafish; C099222:hoxd12a protein, zebrafish; C491715:ptch1 protein, zebrafish; C092119:Hoxa10 protein, mouse; D014212:Tretinoin; C000541:cyclopamine