Effects of gadolinium ions upon rat prostatic cancer cell lines of markedly different metastatic potential.
The effects of gadolinium chloride, a non-specific blocker of mechanosensitive ion channels (MSICs), upon the motility and proliferation of two Dunning rat prostatic tumour cell lines of markedly different metastatic potential were investigated. Gadolinium (2-10 microM) caused a dose-dependent increase in the distance moved in 'wound' assays over a 48-h testing period. The highly metastatic MAT-LyLu cell line was significantly more sensitive to Gd3+, the weakly metastatic AT-2 cells responding only at the highest concentration (10 microM) used. There was no effect on the cells' proliferative rates. These data suggest that mechanosensitive channels could play a role in metastasis by modulating cell migration.
Verrall
J
J
Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.
Fraser
S P
SP
Djamgoz
M B
MB
D000818:Animals; D001696:Biomechanical Phenomena; D002277:Carcinoma / Q000188:drug therapy* / Q000556:secondary; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005682:Gadolinium / Q000627:therapeutic use*; D007473:Ion Channels / Q000037:antagonists & inhibitors*; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D014407:Tumor Cells, Cultured
D007473:Ion Channels; D005682:Gadolinium; C038958:gadolinium chloride