Effects of schwann cell suspension grafts on axon regeneration in subacute and chronic CNS traumatic injuries.
In a previous study, we have shown that microtransplanted Schwann cell suspensions foster structural recovery of the acutely transected postcommissural fornix. The emphasis of the present study was to examine whether subacutely and chronically injured axons also demonstrate significant responsiveness to implanted Schwann cells. Microinjected suspensions of cultured Schwann cells i) elicited a growth response and attracted axons in a subacute and chronic traumatic lesion but ii) failed to stimulate regrowth of the postcommissural fornix projection at any nonacute postlesion stage. In conclusion, the single intervention strategy of Schwann cell microimplantation is not sufficient to ensure regeneration of the subacutally or chronically transected postcommissural fornix. The use of Schwann cells as stimulators of axon regrowth depends on the neuronal cell type and the appropriate postinjury time point.
Stichel
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CC
Molecular Neurobiology Laboratory, Department of Neurology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany. christine.stichel-gunkel@ruhr-uni-bochum.de
Hermanns
S
S
Lausberg
F
F
Müller
H W
HW
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D016380:Brain Tissue Transplantation; D002478:Cells, Cultured; D003911:Dextrans; D020712:Fornix, Brain / Q000601:surgery; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D008297:Male; D020932:Nerve Growth Factor / Q000032:analysis; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000637:transplantation*; D013997:Time Factors; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D003911:Dextrans; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D016900:Neurofilament Proteins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; C076397:biotinylated dextran amine; D001710:Biotin; D020932:Nerve Growth Factor