Low M(r) phosphotyrosine protein phosphatase activity on fibroblast growth factor receptor is not associated with enzyme translocation.
Fibroblast growth factor receptor (class IV) shares a certain degree of similarity with class III members like platelet-derived growth factor and macrophage-colony-stimulating factor receptors, which, once activated, are substrates of low M(r) phosphotyrosine protein phosphatase. Up until now no phosphotyrosine phosphatase has been shown to act on this receptor in vivo. Here we demonstrate that low M(r) phosphotyrosine protein phosphatase is able to reduce receptor tyrosine phosphorylation and cell proliferation in response to basic fibroblast growth factor. Contrary to what was previously observed for platelet-derived growth factor, during cell stimulation with basic fibroblast growth factor, no enzyme redistribution among cellular compartments is observed.
Rigacci
S
S
Department of Biochemical Sciences, University of Firenze, Viale Morgagni 50, 50134, Florence, Italy.
Rovida
E
E
Bagnoli
S
S
Dello Sbarba
P
P
Berti
A
A
D016475:3T3 Cells; D000818:Animals; D001692:Biological Transport; D005346:Fibroblast Growth Factors / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*; D015398:Signal Transduction
D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D016173:Macrophage Colony-Stimulating Factor; C490428:Acp1 protein, mouse; D017027:Protein Tyrosine Phosphatases