Determination of indole-3-acetic acid, tryptophan and other indoles in must and wine by high-performance liquid chromatography with fluorescence detection.
The development of a robust method to analyse the content of tryptophan and of indole-3-acetic acid at the microgram per litre level in must and wine is necessary in order to study the formation of 2-aminoacetophenone and of other indole compounds causing the 'untypical ageing off-flavour'. The present paper discusses the development and validation of a reversed-phase high-performance liquid chromatography method with fluorescence detection for the analysis of indole-3-acetic acid, tryptophan, tryptophol, indole and skatole in must and white wine. The required selectivity and sensitivity was gained through the solid-phase extraction on a polystyrene-based polymer column.
Mattivi
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F
Istituto Agrario di S. Michele, Dip. Laboratorio Analisi e Ricerche, Ljubljana, Slovenia. fulvio.mattivi@ismaa.it
Vrhovsek
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U
Versini
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G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007210:Indoleacetic Acids / Q000032:analysis*; D007211:Indoles / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D007210:Indoleacetic Acids; D007211:Indoles; C030737:indoleacetic acid; D014364:Tryptophan