title: The yeast Saccharomyces cerevisiae does not sequester chloride but can express a functional mammalian chloride channel.
abstract: Chloride uptake into yeast was measured as a function of pH. A small amount of uptake was seen at pH values of 3.0 and 4.0; at pH 6.0 chloride uptake was substantially less than the uptake of phosphate and rubidium. Because chloride uptake is inefficient, we expressed the putative mammalian chloride channel, pI(Cln), in yeast and observed a chloride-selective current when total membrane protein was reconstituted into lipid bilayers. The current was inhibited by a specific chloride channel blocker, 5-nitro-2-(3-phenylpropylamino)-benzoic acid. These results suggest that yeast may serve as a means to characterize chloride channels from other organisms.
authors.affiliation: Renal Electrolyte Division, Department of Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA 15213-2500, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Coury
authors.forename: J E
authors.initials: JE
authors.lastname: McGeoch
authors.forename: G
authors.initials: G
authors.lastname: Guidotti
authors.forename: J L
authors.initials: JL
authors.lastname: Brodsky
mesh_terms: D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D010710:Phosphates