title: Micro-perfusion flow cell for imaging cultured cells.
abstract: We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.
authors.affiliation: University of Georgia, Athens, USA.
authors.forename: N
authors.initials: N
authors.lastname: Agnihotri
authors.forename: W S
authors.initials: WS
authors.lastname: Kisaalita
authors.forename: C H
authors.initials: CH
authors.lastname: Keith
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002468:Cell Physiological Phenomena; D000242:Cyclic AMP / Q000378:metabolism; D003584:Cytological Techniques / Q000295:instrumentation; D019793:Fluorescein; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D016716:PC12 Cells; D010477:Perfusion; D051381:Rats; D012235:Rhodamines; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine; D000242:Cyclic AMP; D019793:Fluorescein