Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) DNA sequences are absent in leukapheresis products and ex vivo expanded CD34+ cells from multiple myeloma patients.
Recently it was reported that Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) infects bone marrow (BM) dendritic cells (DC) in multiple myeloma (MM) patients and therefore might play a role in MM development. Because of the use of myeloid growth factors like GM-CSF and G-CSF for the mobilization of peripheral blood progenitor cells (PBPC), the subsequent increase of DC precursors might imply a risk for KSHV contamination in PBPC grafts. Therefore, in this study leukapheresis products and ex vivo cultured CD34+ cell suspensions were analysed. KSHV DNA could not be amplified in any of them.
De Greef
C
C
Department of Haematology-Immunology, Free University of Brussels, Belgium.
Van De Voorde
W
W
Bakkus
M
M
Corthals
J
J
Heirman
C
C
Schots
R
R
Lacor
P
P
Van Camp
B
B
Van Riet
I
I
D018952:Antigens, CD34; D004279:DNA, Viral / Q000032:analysis*; D016679:Genome, Viral; D019650:Hematopoietic Stem Cell Mobilization; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007937:Leukapheresis; D009101:Multiple Myeloma / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D018952:Antigens, CD34; D004279:DNA, Viral