Expression and localization of the proteoglycan decorin during the progression of cholesterol induced atherosclerosis in Japanese quail: implications for interaction with collagen type I and lipoproteins.
The temporal and spatial distribution, and relative levels of the proteoglycan decorin and collagen type I were examined during the progression of atherosclerosis in the dorsal aortas of Japanese quail selected for cholesterol induced atherosclerosis. The quail were placed on either a control or 0.5% added cholesterol diet at approximately 16 weeks of age. Dorsal aortas were collected at 1- or 2-week intervals over a 15-week period after initiating cholesterol feeding. Biochemical analysis for decorin and collagen type I showed that both increased in the cholesterol-fed birds compared to control-fed birds beginning at 9 weeks and continued through the duration of the study. Through immunohistochemical staining for decorin and collagen type I, the spatial localization of decorin and collagen type I in control and less severe plaques in cholesterol-fed birds was most prominent in the arterial adventitia. However, in severe atherosclerotic plaques, decorin was localized in foam cell regions and collagen type I was found surrounding the foam cell regions where decorin accumulated. These results demonstrated a localization of decorin in the core of the atherosclerotic plaque foam cell region with collagen type I being located on the plaque surface.
Jarrold
B B
BB
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA.
Bacon
W L
WL
Velleman
S G
SG
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001018:Aortic Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D001161:Arteriosclerosis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000633:toxicity; D003094:Collagen / Q000378:metabolism*; D003370:Coturnix; D058575:Decorin; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D008074:Lipoproteins / Q000378:metabolism*; D008297:Male; D011509:Proteoglycans / Q000096:biosynthesis*; D011897:Random Allocation; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*
D002791:Cholesterol, Dietary; D058575:Decorin; D016326:Extracellular Matrix Proteins; D008074:Lipoproteins; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D003094:Collagen; D002784:Cholesterol