Production of (R)-3-pentyn-2-ol through stereoinversion of racemic 3-pentyn-2-ol by Nocardia fusca AKU 2123.
Wet cells of Nocardia fusca AKU 2123 are good catalysts for the production of (R)-3-pentyn-2-ol (PYOH) from (RS)-PYOH through a stereoinversion reaction. Under optimal conditions (350 mM potassium phosphate buffer, pH 8.0, 30% (w/v) wet cells, 0.12% NADPH, 10% glucose, and 30 U/ml glucose dehydrogenase) (R)-PYOH of high optical purity (98.7% e.e.) was produced from 2% (v/v) (RS)-PYOH with a yield of 70.4% by 140 h incubation.
Xie
S X
SX
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Ogawa
J
J
Shimizu
S
S
D000480:Alkynes / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009615:Nocardia / Q000378:metabolism*; D013237:Stereoisomerism
C401284:3-pentyn-2-ol; D000480:Alkynes; D005947:Glucose