Sequencing analysis of forty-eight human image cDNA clones similar to Drosophila mutant protein.
We have sequenced 48 human IMAGE cDNA clones selected from the public EST database (dbEST) for their significant homology to Drosophila mutant genes. A dynamically updated analysis report was produced by BlastX and BlastN analysis searches in the latest databases available. This analysis led us to estimate the grade of similarity with homologous genes isolated in other species. Bottlenecks were detected in the sequencing process and here we have presented our problem-solving approach. We think the value of this full-length sequencing project is an enrichment of the sequence database information that is currently available to the human genome community.
Volorio
S
S
Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy.
Simon
G
G
Repetto
M
M
Cucciardi
M
M
Banfi
S
S
Borsani
G
G
Ballabio
A
A
Zollo
M
M
D000818:Animals; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D020224:Expressed Sequence Tags; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary