title: Experimental unilateral undescended testis: gubernaculectomy and anchoring or direct suture fixation?
abstract: This study was designed to evaluate whether creation of a unilateral undescended testis (U/L UDT) in rats by direct fixation of the testis can lead to changes in the contralateral (C/L) descended testis, and if so, whether this inherent problem of the model could be eliminated by anchoring the divided gubernaculum to indirectly fix the testis. Thirty male newborn rats were divided into three groups of 10 each and the procedure done on the 2nd day of life to create U/L UDT according to the group allocated: group I: sham-operated; group II: anchoring the gubernaculum after gubernaculectomy; group III: Direct suture fixation of the testis. Fertility, C/L testicular weight (TW), Johnsen score, seminiferous tubular diameter (STD), DNA flow cytometry, and serum anti-sperm antibodies (ASA) were studied. Fertility, C/L TW, Johnsen score, STD, and haploid cell population were significantly reduced in group III compared to group II, while significantly higher titers of ASA were found in group III. Gubernaculectomy and anchoring the gubernaculum to the anterior abdominal wall is a better technique of creation of experimental UDT, as direct fixation of the testis is potentially detrimental to the C/L normal, descended testis.
authors.affiliation: Department of Paediatric Surgery, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Srinivas
authors.forename: S
authors.initials: S
authors.lastname: Agarwala
authors.forename: S
authors.initials: S
authors.lastname: Datta Gupta
authors.forename: S N
authors.initials: SN
authors.lastname: Das
authors.forename: C
authors.initials: C
authors.lastname: Shaha
authors.forename: D K
authors.initials: DK
authors.lastname: Mitra
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003456:Cryptorchidism / Q000601:surgery*; D004195:Disease Models, Animal; D005298:Fertility; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000166:cytology; D013536:Suture Techniques; D013737:Testis / Q000166:cytology