A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry.
A new method using GC-MS was devised for the convenient measurement of formate dehydrogenase (FDH) activity in crude tissue samples. FDH activity was detected by measuring headspace 13CO2, which was enzymically converted from [13C]formic acid. This method proved to be sensitive and simple for the estimation of FDH activity without complicated pretreatment.
Shiraishi
T
T
Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
Fukusaki
E
E
Kajiyama
S
S
Kobayashi
A
A
D005560:Formate Dehydrogenases / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012275:Oryza / Q000201:enzymology; D012680:Sensitivity and Specificity
D005560:Formate Dehydrogenases