No clastogenic activity of a senna extract in the mouse micronucleus assay.
In previous studies, an analytically well-defined senna extract, commonly used as a laxative, gave positive responses in vitro in the Ames test and in the CHO assay. Therefore, the objective of this study was to investigate the genotoxic activity of the same senna extract in an in vivo genotoxicity assay by means of the generally acknowledged MNT. After administration of an oral dose of 2000 mg senna extract/kg to NMRI mice of both genders, which is equivalent to 119 mg potential rhein/kg, 5.74 mg potential aloeemodin/kg and 0. 28 mg potential emodin/kg, there were no elevated levels of micronuclei in bone marrow cells. Kinetic studies were performed in parallel to demonstrate target organ availability. Highest concentrations in the plasma were reached after 1 h with 3.4 microg rhein/ml and 0.065 microg aloeemodin/ml. In all cases, emodin was below the limit of quantification. From the results, the in vitro clastogenic activity of the senna extract could not be confirmed in the mouse micronucleus assay. Together with further negative in vivo genotoxicity studies with anthranoids, the conclusion can be drawn that there is no indication so far demonstrating a genotoxic risk for patients taking senna laxatives.
Mengs
U
U
Madaus, Ostmerheimer Strasse 198, D-51109, Cologne, Germany.
Grimminger
W
W
Krumbiegel
G
G
Schuler
D
D
Silber
W
W
Völkner
W
W
D000818:Animals; D002400:Cathartics / Q000633:toxicity*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity; D012676:Senna Extract / Q000493:pharmacokinetics / Q000633:toxicity*
D002400:Cathartics; D009153:Mutagens; D010936:Plant Extracts; D012676:Senna Extract