title: A novel glycosphingolipid from gram-negative aquatic bacteria.
abstract: The chloroform-methanol extractable lipids of the Gram-negative fresh-water bacteria Arcocella aquatica NO-502 and Flectobacillus major FM were found to contain an unusual ninhydrin-positive glycolipid. It was purified by two-stage silica gel-column chromatography. By the use of IR and (1)H-NMR spectroscopy, mass spectrometry and chemical-degradation experiment, the lipid was established to be 1-O-monoglycosyl ceramide, the carbohydrate moiety of which was the alpha-pyranose-ring form of 7-desoxy-7-amino-D-manno-heptulosonic acid, or 1-hydroxycarbonyl-6-deoxy-6-amino-alpha-D-mannopyranose. The ceramide portion consisted mainly (by 95% in the A. aquatica glycolipid and 80% in the F. major glycolipid) of 2-N-(2'-D-hydroxy-13'-methyltetradecanoyl)-15-methyl-4(E)-hexad ecasph ingenine. The minor molecular species differed from the major one only in fatty acid structure. The glycolipid accounted for 8 and 11% of the total lipids extracted from A. aquatica NO-502 and F. major FM cells, respectively.
authors.affiliation: Research Centre 'Hydrobios' of Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Batrakov
authors.forename: V I
authors.initials: VI
authors.lastname: Sheichenko
authors.forename: D I
authors.initials: DI
authors.lastname: Nikitin
mesh_terms: D006028:Glycosphingolipids / Q000737:chemistry* / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared
chemical_list: D006028:Glycosphingolipids