title: Estradiol-17beta stimulates the renewal of spermatogonial stem cells in males.
abstract: In this work, we examined the functions of the female hormone "estrogen" on spermatogenesis of the Japanese eel (Anguilla japonica). Estradiol-17beta (E(2)), a natural estrogen in vertebrates, was present in the serum and its receptor was expressed in the testis during the whole process of spermatogenesis. Spermatogonial stem cell renewal was promoted by E(2) implantation but was suppressed by tamoxifen (an antagonist of estrogen). In vitro, 10 pg/ml of E(2) was sufficient to induce spermatogonial stem cell division in cultured testicular tissue, therefore confirming the in vivo observations. These results clearly show that estrogen is an indispensable "male hormone" in the early spermatogenetic cycle.
authors.affiliation: Faculty of Fisheries, Hokkaido University, Hakodate, 041-8611, Japan. takeshi@pop.fish.hokudai.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: C
authors.initials: C
authors.lastname: Miura
authors.forename: T
authors.initials: T
authors.lastname: Ohta
authors.forename: M R
authors.initials: MR
authors.lastname: Nader
authors.forename: T
authors.initials: T
authors.lastname: Todo
authors.forename: K
authors.initials: K
authors.lastname: Yamauchi
mesh_terms: D000811:Anguilla; D000818:Animals; D006063:Chorionic Gonadotropin / Q000502:physiology; D004958:Estradiol / Q000502:physiology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000097:blood; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000096:biosynthesis; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*; D013093:Spermatogonia / Q000502:physiology*; D013629:Tamoxifen / Q000494:pharmacology; D013737:Testis / Q000502:physiology; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D004965:Estrogen Antagonists; D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D013629:Tamoxifen; D004958:Estradiol