The detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, by gas chromatography/mass spectrometry.
A sensitive gas chromatography/mass spectrometry method for the detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, is described. The method is based upon solvent extraction followed by derivatisation with methyl-bis-trifluoroacetamide (MBTFA), to give a trifluoroacetyl derivative. A profile of 7-aminoflunitrazepam urinary concentrations following ingestion of 0.5 to 4 mg oral doses is reported. The method has been found to be reproducible and can be used for the confirmation of flunitrazepam administration.
Feely
J
J
Department of Pharmacology and Therapeutics, Trinity College, Dublin.
Kavanagh
P V
PV
McNamara
S M
SM
D002849:Chromatography, Gas; D005445:Flunitrazepam / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry
D005445:Flunitrazepam; C036343:7-aminoflunitrazepam