title: Crystallization and preliminary crystallographic analysis of major nitroreductase from Escherichia coli.
abstract: NADPH:nitrocompound oxidoreductase from Escherichia coli, NfsA, has been crystallized in the presence of FMN by the vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belonged to the triclinic space group P1 with cell dimensions, a = 52.2, b = 52.7, c = 53.3 A, alpha = 75.1, beta = 60.1, gamma = 60.5 degrees. The crystals are expected to contain two NfsA molecules per asymmetric unit. The crystals diffracted X-rays to at least 2.3 A resolution and are appropriate for structural analysis at high resolution.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobori
authors.forename: W C
authors.initials: WC
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Akagi
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: S
authors.initials: S
authors.lastname: Zenno
authors.forename: K
authors.initials: K
authors.lastname: Saigo
authors.forename: M
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D019008:Drug Resistance, Neoplasm; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005486:Flavin Mononucleotide / Q000737:chemistry; D009249:NADP / Q000737:chemistry; D009601:Nitroreductases / Q000737:chemistry*; D011092:Polyethylene Glycols; D012984:Software
chemical_list: D029968:Escherichia coli Proteins; D011092:Polyethylene Glycols; D009249:NADP; D005486:Flavin Mononucleotide; C116053:NfsA protein, E coli; D009601:Nitroreductases