Effects of subclinical bovine paratuberculosis on in-vitro polymorphonuclear neutrophil migration.
Migration of polymorphonuclear neutrophils (PMNs) [unstimulated or stimulated with zymosan-activated serum (ZAS)] from 18 cows was measured in a microwell filter assay. Of these animals, 10 were subclinically infected with Mycobacterium paratuberculosis and shown by culture to be excreting the organism in the faeces; the remaining eight were clinically normal and negative for M. paratuberculosis on faecal culture. PMN "net migration" (stimulated minus unstimulated cells) of the infected cows was significantly lower than that of the uninfected cows. Migration of unstimulated cells in the infected cows did not differ from that in the uninfected cows. It would therefore appear that the infection influenced only the migratory response of the ZAS-stimulated cells. 1999 Harcourt Publishers Ltd.
Dotta
U
U
Department of Animal Pathology, University of Turin, Turin, 10126, Italy.
Guglielmino
R
R
Cagnasso
A
A
D'Angelo
A
A
Prato
S
S
Bosso
M
M
D000818:Animals; D002417:Cattle; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005243:Feces / Q000382:microbiology; D005260:Female; D066298:In Vitro Techniques; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology*; D010283:Paratuberculosis / Q000276:immunology*; D015054:Zymosan / Q000494:pharmacology
D015054:Zymosan