title: The exon-intron organization of the prohormone convertase PC2 gene from the insect Lucilia cuprina.
abstract: Prohormone or proprotein convertases are members of the subtilisin family of serine proteases. They are involved in the activation of precursor molecules by endoproteolytic cleavage at basic amino acid residues. Among the different members of this prohormone convertase family, the prohormone convertase 2 (PC2) is almost exclusively expressed in endocrine and neuroendocrine tissues and plays an important role in the endoproteolytic processing of prohormones. Here we describe the exon-intron organization of the PC2 gene from the insect Lucilia cuprina by characterization of PCR-amplified genomic DNA fragments. The insect PC2 gene contains 12 exons with an estimated size of over 14.5 kb. The exon sizes range from 38 bp to > 448 bp. All identified intron-exon boundaries are consistent with the GT-AG-rule. A comparison of the genomic structures of the thus far known prohormone convertase genes with that of the insect PC2 gene revealed a conservation of the positions of most introns interrupting the exons coding for the amino-terminal and catalytic domains. This conservation is consistent with the suggestion of a common evolutionary origin for the prohormone convertase gene family.
authors.affiliation: Department of General Zoology and Endocrinology, University of Ulm, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Mentrup
authors.forename: W
authors.initials: W
authors.lastname: Weidemann
mesh_terms: D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D017124:Conserved Sequence; D004175:Diptera / Q000235:genetics*; D019143:Evolution, Molecular; D005091:Exons; D007438:Introns; D016133:Polymerase Chain Reaction; D045706:Proprotein Convertase 2; D013381:Subtilisins / Q000235:genetics*
chemical_list: D013381:Subtilisins; D045706:Proprotein Convertase 2