C-fos protein expression in the nucleus of the solitary tract correlates with cholecystokinin dose injected and food intake in rats.
C-fos protein expression was investigated in the nucleus of the solitary tract (NTS) in response to increasing cholecystokinin (CCK) doses and food intake in rats by counting the number of c-fos protein positive cells in the NTS. C-fos protein expression in the NTS dose-dependently increased in response to CCK, the lowest effective dose being 0.1 microg/kg. The ED(50) for c-fos protein expression in the NTS in response to CCK was calculated to be 0.5 to 1.8 microg/kg, depending on the anatomical level of the NTS investigated. Food intake increased c-fos protein expression in the NTS, the maximum number of c-fos protein positive cells being reached at 90 min after the start of food intake. Regression analysis identified a positive correlation between c-fos protein expression and the amount of food intake. Our data indicate that subpopulations of the NTS that are activated by CCK or food intake are involved into the short-term regulation of food intake and the neural control of feeding by the caudal brainstem.
Zittel
T T
TT
University Hospital, Department of General and Transplantation Surgery, Hoppe-Seyler-Str. 3, University of Tübingen, Tübingen, Germany. tilzittel@aolc.om
Glatzle
J
J
Kreis
M E
ME
Starlinger
M
M
Eichner
M
M
Raybould
H E
HE
Becker
H D
HD
Jehle
E C
EC
D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000502:physiology*; D007422:Intestines / Q000294:innervation; D016015:Logistic Models; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011949:Receptors, Cholecystokinin / Q000502:physiology; D012527:Satiation / Q000502:physiology; D017552:Solitary Nucleus / Q000187:drug effects* / Q000378:metabolism*; D014630:Vagus Nerve / Q000737:chemistry / Q000502:physiology
D016760:Proto-Oncogene Proteins c-fos; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin