title: The characterization of Shiga toxin-non-producing Escherichia coli serotype O157:H7 isolated from carcasses of cattle at a slaughter house.
abstract: A bacteriological investigation of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 was performed on 298 carcasses of cattle at slaughter houses between July 1996 and January 1997 in Gifu Prefecture, Japan. As a result, four Stx-non-producing Escherichia coli O157:H7 strains were isolated from two slaughtered carcasses of cattle. The purpose of this study was to examine the characterization of isolates. Isolates possessed the E. coli attaching and effacing gene (eaeA), and hemolysin gene (hlyA), and harbored 3.0-MDa and 60-MDa plasmids. The Xba I pulsed-field gel electrophoresis (PFGE) pattern showed three similar patterns. Consequently, a closely related genotype of Stx-non-producing E. coli O157:H7 may widely exist in cattle.
authors.affiliation: Gifu Prefectural Meat Inspection Office, Ohgaki, Japan. mobydick@sam.hi-ho.ne.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Katoh
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: S
authors.initials: S
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D000003:Abattoirs; D018829:Adhesins, Bacterial; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002352:Carrier Proteins; D002417:Cattle / Q000382:microbiology*; D002522:Chlorocebus aethiops; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D022603:Shiga Toxins; D014407:Tumor Cells, Cultured; D014709:Vero Cells
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C104901:BfpA protein, E coli; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D022603:Shiga Toxins; C073819:eaeA protein, E coli; D036781:Fimbriae Proteins