title: Frontal lobe neuronal injury correlates to altered function in FIV-infected cats.
abstract: Six cats infected intravenously at 8 weeks of age with feline immunodeficiency virus Maryland isolate (FIV-MD), were evaluated at 8 and 14 months of age (6 months and 12 months postinfection, respectively) with high spatial resolution proton magnetic resonance spectroscopy (MRS) of the frontal cortex. Two separate control cat groups were evaluated at 8 months and 16 months of age. Single voxel two-dimensional high-resolution proton magnetic resonance imaging was performed using the PRESS sequence by selecting a 0.125 ml volume of interest in the medial frontal cortex. A significant reduction in both N-acetylaspartate (NAA) and NAA: choline ratio was found in the FIV 14-month-old group compared with FIV 8-month-old cats, and to the respective age-matched control 16-month-old cats. A negative correlation between NAA and CD4 lymphocyte count was seen in the FIV-14 group only. This group of FIV cats also exhibited a higher proportion of quantitative electroencephalographic relative slow wave activity (RSWA) that correlated to lower NAA content in the frontal cortical voxel. Although peripheral blood proviral load increased over time of infection, no correlation was found between proviral blood or lymph node load and NAA values, CD4 lymphocyte counts, or frontal cortical RSWA. Thus, this study demonstrated that neurologic functional disruption of the frontal cortex correlated strongly with neuronal injury and/or loss in FIV-MD-infected cats independent of peripheral proviral load. The ability to define in vivo neurodegeneration further in this animal model helps in understanding the neuropathogenesis of lentivirus infection, and possibly, a means to follow progression and reversibility during the initial stages of brain infection as therapeutic agents are identified.
authors.affiliation: Department of Veterinary Clinical Sciences, The Ohio State University, Columbus 43210, USA.
authors.forename: M
authors.initials: M
authors.lastname: Podell
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: K A
authors.initials: KA
authors.lastname: Hayes
authors.forename: W R
authors.initials: WR
authors.lastname: Buck
authors.forename: D S
authors.initials: DS
authors.lastname: Ruehlmann
authors.forename: L E
authors.initials: LE
authors.lastname: Mathes
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D018791:CD4 Lymphocyte Count; D002415:Cats; D002794:Choline / Q000032:analysis; D004569:Electroencephalography; D016181:Feline Acquired Immunodeficiency Syndrome / Q000473:pathology* / Q000503:physiopathology; D005625:Frontal Lobe / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology; D018698:Glutamic Acid / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D016089:Immunodeficiency Virus, Feline; D008279:Magnetic Resonance Imaging; D009474:Neurons / Q000473:pathology; D013047:Specific Pathogen-Free Organisms
chemical_list: D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; C000179:N-acetylaspartate; D002794:Choline