title: Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w.
abstract: We have carried out the systematic disruption of six ORFs on chromosome XV, of Saccharomyces cerevisiae using the long flanking homology technique to replace each with the KanMX cassette; we have also constructed plasmids containing replacement cassettes and cognate clones for each ORF. Disruption of three of the ORFs-YOL117w, YOL114c, and YOL112w (also known as MSB4)-does not result in any noteworthy phenotype with respect to temperature or nutritional requirements, but yol112w mutants with an additional disruption of YNL293w, which encodes a protein similar to Yol112w, exhibit a slow growth phenotype. The protein specified by YOL114c shares similarity with the human DS-1 protein. Disruption of YOL115w confers slow growth, cold sensitivity and poor sporulation; this ORF has been described elsewhere as TRF4, which encodes a topoisomerase I-related protein. Cells with disruptions of YOL111c, whose product is weakly similar to the human ubiquitin-like protein GdX, are slightly impaired in mating. Mutants disrupted for YOL072w, the predicted product of which is unrelated to any protein of known function, grow slowly, are cold-sensitive and sporulate with reduced efficiency.
authors.affiliation: Institut Curie, Section de Recherche, CNRS UMR 144, 26 Rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: L
authors.initials: L
authors.lastname: Iwanejko
authors.forename: K N
authors.initials: KN
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Loeillet
authors.forename: A
authors.initials: A
authors.lastname: Nicolas
authors.forename: F
authors.initials: F
authors.lastname: Fabre
mesh_terms: D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D015825:Chromosomes, Fungal; D004259:DNA-Directed DNA Polymerase; D009687:Nuclear Proteins; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D004259:DNA-Directed DNA Polymerase; C487545:PAP2 protein, S cerevisiae