Evaluation of specific IgE to the recombinant group 2 mite allergens Lep d 2 and Tyr p 2 in the Pharmacia CAP system.
BACKGROUND
Several recombinant allergens have been shown to be potentially useful for diagnosis of IgE-mediated allergy, but only a few recombinant allergens are at present commercially available in serological assays for detection of specific IgE. The aim of this study was to evaluate the IgE binding to the recombinant major dust mite allergens rLep d 2 and rTyr p 2 and compare it with the IgE binding to the commercial mite extracts Lepidoglyphus destructor and Tyrophagus putrescentiae in the Pharmacia RAST CAP System.


METHODS
The recombinant allergens rLep d 2 and rTyr p 2 were immobilised on ImmunoCAPs, and sera from 461 Swedish farmers who are frequently exposed to mites were analysed for specific IgE antibodies. Immunoblotting was performed to evaluate discrepancies between the results obtained with the recombinant and the commercial CAP assays.


RESULTS
The IgE values of each recombinant assay significantly correlated with the IgE values of the corresponding commercial CAP assay. The sensitivity of the rLep d 2 assay was 73.3% and that of the rTyr p 2 assay, 60.5% of that provided by the commercial L. destructor and T. putrescentiae assays. Two subjects out of 416, who tested negative in the commercial L. destructor assay, were positive to rLep d 2. The corresponding figures for rTyr p 2 and the T. putrescentiae extract were 5/418. The possibility that these subjects were sensitised to L. destructor and T. putrescentiae could not be excluded.


CONCLUSIONS
The data suggest that it may be possible to use rLep d 2 and rTyr p 2 on ImmunoCAPs to detect and quantify IgE antibodies to these, the major allergens of L. destructor and T. putrescentiae. It appears likely that the addition of just a few more recombinant L. destructor and T. putrescentiae allergens in the CAP assay will be sufficient for in vitro diagnosis of IgE mediated allergy to L. destructor and T. putrescentiae.
Johansson
E
E
Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska Institute and Hospital, Stockholm, Sweden. eva.johansson@ mb.ks.de
Eriksson
T L
TL
Olsson
S
S
Kronqvist
M
M
Whitley
P
P
Johansson
S G
SG
Gafvelin
G
G
van Hage-Hamsten
M
M
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D005069:Evaluation Studies as Topic; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood*; D008925:Mites / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D011852:Radioallergosorbent Test / Q000379:methods*; D011994:Recombinant Proteins / Q000276:immunology
D000485:Allergens; C090039:Lep d I allergen; D011506:Proteins; D011994:Recombinant Proteins; C110921:Tyr p 2 protein, Tyrophagus putrescentiae; D007073:Immunoglobulin E