Transgene as vaccine for chlamydia.
BACKGROUND
We evaluated the potential utility of DNA immunization with the major outer membrane protein (MOMP) gene of Chlamydia trachomatis mouse pneumonitis (MoPn) strain for induction of protective immunity to chlamydial infection in mice.


RESULTS
Groups of Balb/c mice were immunized with naked DNA intramuscularly or intranasally or with MOMP DNA-transfected Salmonella typhimurium delivery orally. Mice were challenged with MoPn through the pulmonary route to assay for protective immunity. All 3 routes of DNA immunization elicited protective immunity. Mucosal delivery appeared more efficacious than intramuscular delivery.


CONCLUSIONS
DNA immunization with the chlamydia MOMP gene may be suitable for vaccine development.
Brunham
R C
RC
Department of Medical Microbiology, University of Manitoba. Winnipeg, Canada.
Zhang
Dj
Dj
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D002690:Chlamydia Infections / Q000097:blood / Q000276:immunology* / Q000517:prevention & control*; D002692:Chlamydia trachomatis / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004333:Drug Administration Routes; D004353:Drug Evaluation, Preclinical; D018928:Immunity, Mucosal / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000627:therapeutic use; D018272:Porins; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D019076:Transgenes / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D019444:Vaccines, DNA; C071677:omp1 protein, Chlamydia trachomatis