Separation and purification of isoflavones from Pueraria lobata by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was applied to the semipreparative separation and purification of puerarin and related isoflavones from a crude extract of Pueraria lobata. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). Using the above solvent system the preparative HSCCC was successfully performed yielding six relatively pure isoflavones including puerarin from 80 mg of the crude extract in one-step separation.
Cao
X
X
Beijing Institute of New Technology Application, China.
Tian
Y
Y
Zhang
T
T
Li
X
X
Ito
Y
Y
D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry*
D004365:Drugs, Chinese Herbal