Increased recovery of swim-up spermatozoa by application of "antigravitational" centrifugation.
OBJECTIVE
To compare the quantity and quality of sperm recovered following the 10-minute application of an antigravitational force during the swim-up procedure with the standard 60-minute swim-up (SSU) procedure.


METHODS
Prospectively controlled in vitro study.


METHODS
Private andrology laboratory and hospital-based infertility practice.


METHODS
Equal aliquots of semen were evaluated following various intervals of antigravitational centrifugation swim-up (ACSU). ACSU and SSU were then compared.


METHODS
Thirty-eight men undergoing therapeutic testing.


METHODS
Sperm concentration, sperm motility, and progressive sperm motility.


RESULTS
The number of sperm recovered from the ACSU procedure was significantly higher than from the SSU procedure. No significant differences in percent motile sperm and progressive motile sperm recovery between the two procedures were observed.


CONCLUSIONS
The ACSU procedure yields a higher number of motile spermatozoa in a much shorter time.
Babbo
C J
CJ
Division of Reproductive Endocrinology, Aultman Hospital, Canton, Ohio 44710, USA.
Hecht
B R
BR
Jeyendran
R S
RS
D002469:Cell Separation; D002498:Centrifugation / Q000379:methods*; D005260:Female; D006112:Gravitation; D006801:Humans; D008297:Male; D011446:Prospective Studies; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*