title: Storage conditions for serum deslorelin.
abstract: The stability of a luteinizing hormone-releasing hormone (LHRH) analog in rat serum was studied under reproduced experimental analysis conditions. Serum samples of deslorelin [D-Trp6, Des-Gly, NH2(10)] LHRH ethylamide were exposed to multiple freeze-thaw cycles to determine the maximum number of cycles the serum sample can be exposed to without producing any quantitative changes in radioimmunoassay (RIA) measurements of deslorelin. A significant cycle effect was observed after completion of the sixth cycle. Serum samples were also stored at standardized -50 degrees C conditions for variable periods of time to determine the effects of acute and chronic storage time on deslorelin stability. Matched-pair t-test analysis showed no significant changes in deslorelin values as measured by RIA for a 3-week, 4-month, or 2-year storage period. The conditions in which the rat serum samples were stored prior to analytical analysis were sufficient to prevent detectable degradation of the deslorelin peptide.
authors.affiliation: University of Rhode Island, Kingston 02881, USA.
authors.forename: L
authors.initials: L
authors.lastname: Underhill
authors.forename: R C
authors.initials: RC
authors.lastname: Rhodes
authors.forename: C T
authors.initials: CT
authors.lastname: Rhodes
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000097:blood*; D005615:Freezing; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000097:blood; D006358:Hot Temperature; D006801:Humans; D011863:Radioimmunoassay; D051381:Rats; D015203:Reproducibility of Results; D013997:Time Factors; D017329:Triptorelin Pamoate / Q000031:analogs & derivatives
chemical_list: D004791:Enzyme Inhibitors; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; C066438:deslorelin