Inhibitory effect of a gastrin receptor antagonist, CR2945, on 1, 2-dimethylhydrazine-induced colorectal cancer in mice.
This study tested the effect of a new gastrin receptor antagonist, CR2945, on colorectal cancer induced by 1,2-dimethylhydrazine (DMH) in mice. 75 CD1 male mice were divided into 3 groups: group 1 received 1 weekly injection of 20 mg/kg of DMH and 2 daily intraperitoneal injections of 0.5 ml of NaCl 0.9% solution for 5 weeks; groups 2 and 3 received the same weekly dose of DMH and 2 daily injections of CR2945 at the respective doses of 2.5 and 7.5 mg/kg for 5 weeks. The animals were sacrificed 25 and 38 weeks after the first injection. No tumours were found at the 25th week. A lower cancer frequency (4%) was observed in treated animals compared to controls (37.4%) at the 38th week (p = 0.002). These data show that CR2945 could prevent chemically induced colon cancer development in mice.
Fontana
M G
MG
Department of Surgery, University of Brescia, Brescia, Italy.
Donato
F
F
Villanacci
V
V
Ghirardi
M
M
Moneghini
D
D
Di Betta
E
E
Salerni
B
B
D019813:1,2-Dimethylhydrazine; D000230:Adenocarcinoma / Q000139:chemically induced / Q000453:epidemiology / Q000473:pathology / Q000517:prevention & control; D000236:Adenoma / Q000139:chemically induced / Q000453:epidemiology / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D001569:Benzodiazepines / Q000494:pharmacology*; D002273:Carcinogens; D015179:Colorectal Neoplasms / Q000139:chemically induced* / Q000453:epidemiology / Q000473:pathology / Q000517:prevention & control*; D015994:Incidence; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors*
C116183:CR 2945; D002273:Carcinogens; D011949:Receptors, Cholecystokinin; D001569:Benzodiazepines; D019813:1,2-Dimethylhydrazine