title: Abnormal osteogenesis in osteoporotic patients is reflected by altered mesenchymal stem cells dynamics.
abstract: Bone marrow contains a population of mesenchymal stem cells with the ability to differentiate into cells that form bone, cartilage, adipose, and other connective tissues. Stem cells can be isolated from bone marrow aspirates and expanded in vitro. Presently, most stem cells studies have been performed in cells obtained from "healthy" control subjects. The goal of this study was to compare the functional characteristics of mesenchymal stem cells derived from "healthy" control and osteoporotic postmenopausal women to better understand the mechanisms involved in the pathogenesis of this disease. Osteoporotic and control stem cells have similar morphology and size and express similar cell surface antigens as evidenced by their reactivity with cell specific monoclonal antibodies. Mesenchymal stem cells from osteoporotic women differ from controls in having a lower growth rate than control cells, being refractory to the mitogenic effect of IGF-1, and exhibiting a deficient ability to differentiate into the osteogenic linage as evidenced by the alkaline phosphatase activity and calcium phosphate deposition. We conclude that in osteoporosis stem cell growth, proliferative response and osteogenic differentiation are significantly affected. Also, the study of mesenchymal stem cells from osteoporotic postmenopausal women may provide a better understanding of the mechanisms involved in the pathogenesis of the osteoporosis. It may also serve to test in vitro in rapid manner novel new therapeutic strategies.
authors.affiliation: Laboratorio de Biología Celular, Universidad de Chile, Casilla 138-11, Santiago, Chile. jprodrig@uec.inta.uchile.cl
authors.forename: J P
authors.initials: JP
authors.lastname: Rodríguez
authors.forename: S
authors.initials: S
authors.lastname: Garat
authors.forename: H
authors.initials: H
authors.lastname: Gajardo
authors.forename: A M
authors.initials: AM
authors.lastname: Pino
authors.forename: G
authors.initials: G
authors.lastname: Seitz
mesh_terms: D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D010024:Osteoporosis / Q000201:enzymology / Q000473:pathology*
chemical_list: D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase