[Double-strand-primered polymerase chain reaction].
OBJECTIVE
To develop a simple and reliable method for DNA amplification.


METHODS
Double-strand-primers of fragile X mental retardation 1 gene (FMR1) were generated and used to amplify DNAs that were roughly extracted from human peripheral blood.


RESULTS
All products from 15 DNA samples by double-strand-primered polymerase chain reaction (DSP-PCR) were more specific than those amplified by routine PCR.


CONCLUSIONS
The double-strand-primers reported here may increase the specificity of PCR amplification.
Xia
Q
Q
Department of Medical Genetics, The Affiliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P.R.China. zszg@wcums.edu.cn
Zhang
S
S
Ding
L
L
Wu
H
H
D006801:Humans; D016133:Polymerase Chain Reaction