Expression of prestalk and prespore proteins in minute, two-dimensional Dictyostelium slugs.
We show that exceedingly small two-dimensional slugs of Dictyostelium differentiate normally and have an anterior prestalk zone and a posterior prespore zone. Using GFP as a marker attached to the appropriate promoter, prestalk expression is concentrated in the anterior, while prespore expression is produced in the posterior, closely resembling what is found in normal, large slugs.
Bonner
J T
JT
Departments of Ecology and Developmental Biology, and Molecular Biology Princeton University, Princeton, NJ 08544, USA. jtbonner@princeton.edu
Fey
P
P
Cox
E C
EC
D000818:Animals; D004023:Dictyostelium / Q000502:physiology* / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013170:Spores / Q000737:chemistry
D008164:Luminescent Proteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins