Failure of selenomethionine residues in albumin and immunoglobulin G to protect against peroxynitrite.
Selenomethionine has been suggested to protect against peroxynitrite by quenching it in vivo. Selenomethionine is distributed randomly in the methionine pool. Albumin and IgG were purified from plasma of a human being before and after 28 days of supplementation with 400 microg selenium/day as selenomethionine. The albumin contained 1 selenium atom, presumably as selenomethionine, per 8000 methionine residues before supplementation and 1 per 2800 after supplementation. Although this ratio suggested that selenomethionine would not have as great an effect in quenching peroxynitrite as would methionine, direct testing of the albumin and IgG fractions was carried out to assess the ability of these proteins to prevent peroxynitrite oxidation of dihydrorhodamine 123 to rhodamine 123. The ability of the albumin preparations to resist nitration of tyrosine residues was also assessed. The high-selenomethionine preparations of the proteins had no greater effect in quenching the peroxynitrite than did the normal-selenomethionine preparations. These results do not support the proposal that selenomethionine in proteins contributes to in vivo protection against peroxynitrite.
Hondal
R J
RJ
Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Motley
A K
AK
Hill
K E
KE
Burk
R F
RF
D000328:Adult; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000187:drug effects; D008297:Male; D009566:Nitrates / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012643:Selenium / Q000493:pharmacokinetics*; D012645:Selenomethionine; D012709:Serum Albumin / Q000737:chemistry* / Q000187:drug effects
D007074:Immunoglobulin G; D009566:Nitrates; D016877:Oxidants; D012709:Serum Albumin; C039642:peroxynitric acid; D012645:Selenomethionine; D012643:Selenium