title: Cytogenetic study of eight new cases of radiation-induced solid tumors.
abstract: Radiation-induced tumors were selected according to the criteria defined by Cahan (1948) for sarcomas. Cell cultures and/or xenografts in nude mice were performed with biopsies obtained from second primary tumors. Karyotypes of eight tumors were established after R-banding. After comparison with literature data on 15 other cases, two distinct cytogenetic patterns could be distinguished. One was characterized by polyclonal karyotypes, of which a large proportion were simple and carriers of balanced translocations. Another one was characterized by monoclonal chromosome alterations observed in highly aneuploid and complex karyotypes, in which many deletions were observed. These two different patterns could be related to the modality of metaphase harvesting. Polyclonal karyotypes were preferentially observed after long-term cultures, and monoclonal karyotypes after short-term cultures or xenografts. The following scheme of radiation oncogenesis is proposed: a) induction of recessive gene mutations including that of tumor suppressor genes; b) accumulation of genomic alterations in the irradiated tissue with aging, including deletions or mutations of normal alleles from mutated tumor suppressor genes; and c) loss of tumor suppressor gene function and initiation of a multistage tumor development and progression. Polyclonal abnormalities are assumed to exist in noncancerous cells which acquired radiation-induced chromosome aberrations.
authors.affiliation: Service de Radiothérapie A, CNRS-Institut Curie LRC No. 4 CEA, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Chauveinc
authors.forename: A M
authors.initials: AM
authors.lastname: Dutrillaux
authors.forename: P
authors.initials: P
authors.lastname: Validire
authors.forename: E
authors.initials: E
authors.lastname: Padoy
authors.forename: L
authors.initials: L
authors.lastname: Sabatier
authors.forename: J
authors.initials: J
authors.lastname: Couturier
authors.forename: B
authors.initials: B
authors.lastname: Dutrillaux
mesh_terms: D000782:Aneuploidy; D000818:Animals; D016147:Genes, Tumor Suppressor; D006801:Humans; D007621:Karyotyping; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000235:genetics* / Q000473:pathology; D016609:Neoplasms, Second Primary / Q000235:genetics* / Q000473:pathology; D017384:Sequence Deletion