title: The isoprostane, 8-epi-PGF2 alpha, is accumulated in coronary arteries isolated from patients with coronary heart disease.
abstract: OBJECTIVE
  In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n = 23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n = 19) or from healthy heart donors (controls, n = 6).
  
  
  METHODS
  Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2 alpha in the vascular intima and media. In addition, the 8-epi-PGF2 alpha content was determined using a specific immunoassay after extraction and purification.
  
  
  RESULTS
  The immunohistochemical results indicated that 8-epi-PGF2 alpha is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P < 0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too.
  
  
  CONCLUSIONS
  From our findings we conclude that 8-epi-PGF2 alpha is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis.
authors.affiliation: Department of Cardiology, University of Vienna, Austria. mmehrabi@pop3.kard.akh-wien.ac.at
authors.forename: M R
authors.initials: MR
authors.lastname: Mehrabi
authors.forename: C
authors.initials: C
authors.lastname: Ekmekcioglu
authors.forename: F
authors.initials: F
authors.lastname: Tatzber
authors.forename: A
authors.initials: A
authors.lastname: Oguogho
authors.forename: R
authors.initials: R
authors.lastname: Ullrich
authors.forename: A
authors.initials: A
authors.lastname: Morgan
authors.forename: F
authors.initials: F
authors.lastname: Tamaddon
authors.forename: M
authors.initials: M
authors.lastname: Grimm
authors.forename: H D
authors.initials: HD
authors.lastname: Glogar
authors.forename: H
authors.initials: H
authors.lastname: Sinzinger
mesh_terms: D000704:Analysis of Variance; D002311:Cardiomyopathy, Dilated / Q000378:metabolism; D003327:Coronary Disease / Q000378:metabolism* / Q000601:surgery; D003331:Coronary Vessels / Q000737:chemistry*; D016027:Heart Transplantation; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011863:Radioimmunoassay