The nascent polypeptide-associated complex (NAC) of yeast functions in the targeting process of ribosomes to the ER membrane.
We study here the binding of ribosomes to the endoplasmic reticulum (ER) membrane and its dependence on nascent polypeptide-associated complex (NAC). For this, we use an in vitro translation system in combination with isolated microsomes. Importantly, all components in the system are derived from a single source, Saccharomyces cerevisiae. Ribosome nascent chains (RNCs) of the two naturally occurring invertase species (secreted or cytosolic) were prepared in wild-type, delta alpha NAC or delta alpha beta 1 beta 3 NAC translation lysates and tested for binding to the corresponding microsomal membranes. We provide evidence that NAC prevents binding of RNCs without a signal sequence to yeast membranes. In the absence of NAC, signal-less RNCs are able to bind to ER membranes. However, following puromycin treatment, only very few nascent chains translocate into the lumen, as detected by glycosylation.
Wiedmann
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Department of Biochemistry, Humboldt University, Berlin, Germany. brigitte.wiedmann@charite.de
Prehn
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D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D007425:Intracellular Membranes / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D018832:Molecular Chaperones; D016296:Mutagenesis; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000502:physiology*; D043324:beta-Fructofuranosidase
D018832:Molecular Chaperones; D015534:Trans-Activators; C096099:nascent-polypeptide-associated complex; D003208:Concanavalin A; D015500:Chloramphenicol O-Acetyltransferase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase