title: Protease M/neurosin mRNA is expressed in mature oligodendrocytes.
abstract: No proteases have been identified to be oligodendrocyte-specific though there are phenomena in which proteases may be committed. We have cloned a cDNA for mouse homologue of protease M/neurosin, serine protease, and localized its mRNA in the mouse brain. The cDNA and the deduced amino acid sequences were 66% identical to those of human protease M/neurosin. In situ hybridization histochemistry revealed that the mRNA was localized in the mature oligodendrocytes which expressed proteolipid protein mRNA. Developmentally, protease M/neurosin mRNA was expressed by oligodendrocytes 2 to 7 days after the maturation. Thus, protease M/neurosin may be important to the processes occurring after the maturation of oligodendrocytes such as myelination or turnover of the proteins in the myelin.
authors.affiliation: Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamanaka
authors.forename: X
authors.initials: X
authors.lastname: He
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: S
authors.initials: S
authors.lastname: Shiosaka
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D006801:Humans; D007610:Kallikreins; D051379:Mice; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; C103551:KLK6 protein, human; D007610:Kallikreins; C491223:Prss18 protein, mouse; D012697:Serine Endopeptidases