Characterization and cDNA cloning of androgenic gland hormone of the terrestrial isopod Armadillidium vulgare.
The sex differentiation in crustaceans is known to be controlled by a peptide hormone called androgenic gland hormone (AGH). AGH was extracted and purified from the androgenic glands (AGs) of the male isopod Armadillidium vulgare by high-performance liquid chromatography. AGH consisted of two peptide chains and their N-terminal amino acid sequences were determined. A cDNA encoding AGH was cloned by PCR and sequenced. The cDNA had an open reading frame of 432 bp, which encoded a preproAGH consisting of a signal peptide (21 residues), B chain (44 residues), C peptide (46 residues), and A chain (29 residues). Through processing, the A and B chains might form a heterodimer interlinked by disulfide bonds. The A chain possessed a putative N-linked glycosylation site. A Northern blot analysis using the cDNA as a probe detected a hybridization signal with 0.8 kb in the RNA preparation only from the AGs.
Okuno
A
A
Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.
Hasegawa
Y
Y
Ohira
T
T
Katakura
Y
Y
Nagasawa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001135:Armadillos / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones; D011498:Protein Precursors; D012333:RNA, Messenger; C053644:androgenic gland hormone (Armadillidium)