title: Enzyme polymorphism in Pseudomonas aeruginosa strains recovered from cystic fibrosis patients in France.
abstract: Each of 314 strains of Pseudomonas aeruginosa recovered from 87 French cystic fibrosis (CF) patients was typed by multilocus enzyme electrophoresis to investigate the genetic diversity, the relatedness and the molecular epidemiology of strains isolated from cases of chronic pulmonary colonization. Comparison of allele profiles at 18 enzyme loci identified 17 electrophoretic types (ETs). Of the 314 isolates, 290 (92%) were either ET1 (n = 127) or ET2 (n = 163), which differed only at the shikimate dehydrogenase (SKD) locus. The mean genetic diversity (H) was 0.138. These results suggest that there is cross-colonization between patients and/or that two predominant groups of strains are able to colonize French CF patients. Sequential isolates collected from 18 patients during a period of 12-28 months were analysed to assess genomic variability and its relationship to clinical outcome. Six patients were colonized by a stable strain. For the others, double infections or changes in colonization over time were observed. No relationships were detected between the clinical outcome and the persistence of stable isolates, the emergence of transient superinfecting variants, the presence of multiple ETs or the shift of ET during the monitoring.
authors.affiliation: Département de Microbiologie Médicale et Moléculaire, Unité de Bactériologie, CHU Bretonneau, 37044 Tours Cedex 1, France1.
authors.forename: Christian
authors.initials: C
authors.lastname: Martin
authors.affiliation: Institute of Molecular Evolutionary Genetics, Pennsylvania State University Park, PA 16801, USA2.
authors.forename: E Fidelma
authors.initials: EF
authors.lastname: Boyd
authors.affiliation: Département de Microbiologie Médicale et Moléculaire, Unité de Bactériologie, CHU Bretonneau, 37044 Tours Cedex 1, France1.
authors.forename: Roland
authors.initials: R
authors.lastname: Quentin
authors.affiliation: Centre Héliomarin, 19 Boulevard Félix Faure, 17370 Saint Trojan-les-Bains, France3.
authors.forename: Pascal
authors.initials: P
authors.lastname: Massicot
authors.affiliation: Institute of Molecular Evolutionary Genetics, Pennsylvania State University Park, PA 16801, USA2.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Selander
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000382:microbiology*; D004586:Electrophoresis / Q000379:methods*; D004798:Enzymes / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D008297:Male; D011110:Polymorphism, Genetic; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology*
chemical_list: D004798:Enzymes