title: Mass spectrometric identification of increased C16 ceramide levels during apoptosis.
abstract: A variety of molecular changes occur during the process of apoptosis. Much of the recent work has focused on changes in critical cellular proteins, proteins necessary for the initiation and continuation of the apoptotic process. Given the fact that numerous membrane changes occur throughout the apoptotic process, we initiated an investigation aimed at determining the major lipid changes that occurred during programmed cell death. When ionizing radiation was used to initiate the apoptotic process in Jurkat cells, one of the major changes that occurred within 24 h was an increase in a species with a m/z of 572 as determined by negative ion electrospray mass spectrometry. This particular mass ion displayed high performance liquid chromatography characteristics of a neutral lipid species. Further analysis by collision-induced-dissociation tandem mass spectrometry indicated only one daughter species indicative of a Cl adduct and therefore a parental mass of 537. Comparison to a commercial C16 ceramide yielded identical spectra by mass spectrometry (MS) and MS/MS analysis in the negative ion mode. Increases in C16 ceramide levels occurred 2 h after initiation of apoptosis by ionizing radiation, and its accumulation paralleled apoptosis as determined by cellular morphology. Interestingly, radiation-sensitive Jurkat cells displayed increased levels of long term C16 ceramide accumulation, whereas radiation-resistant K562 cells did not. These findings were supported by increases in caspase-3 activity in Jurkat cells, whereas caspase-3 activity in K562 cells remained unchanged. C16 ceramide accumulation and sensitivity to ionizing radiation was investigated further in a melanoma cell line. Only those cells that were radiation sensitive (approximately 70-75%) displayed increases in long term ceramide accumulation. Taken together, these results indicated a correlation between increases in C16 ceramide accumulation and radiation sensitivity. Increases in long term C16 ceramide accumulation were also seen in Fas-induced apoptosis, which occurred at time points greater than 2 h. Analysis of mitochondrial modifications using the mitochondrial probe nonyl acridine orange (NAO) indicated that initial increases in C16 ceramide levels closely paralleled the decrease in mitochondrial mass during Fas or radiation-induced apoptosis. Taken together, these results support a role for C16 ceramide in the effector (mitochondrial) phase of apoptosis.
authors.affiliation: Department of Surgery, Pittsburgh, Pennsylvania 15213, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Thomas
authors.forename: C M
authors.initials: CM
authors.lastname: Matsko
authors.forename: M T
authors.initials: MT
authors.lastname: Lotze
authors.forename: A A
authors.initials: AA
authors.lastname: Amoscato
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002518:Ceramides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology* / Q000502:physiology; D020014:K562 Cells / Q000166:cytology / Q000502:physiology; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D019014:fas Receptor / Q000276:immunology / Q000502:physiology
chemical_list: D002518:Ceramides; D019014:fas Receptor