Superantigen immune stimulation evokes epithelial monocyte chemoattractant protein 1 and RANTES production.
Bacterial superantigens (SAgs) have been implicated in inflammatory disease, and SAg-treated mice have increased jejunal T cells. Here we show that T84 cells (a human epithelial cell line) display increased MCP-1 and RANTES mRNA expression and protein production in response to conditioned medium from Staphylococcus aureus enterotoxin B (SEB; a model SAg)-activated immune cells. Also, MCP-1 and RANTES mRNAs were increased in jejunal enterocytes isolated from SEB-treated mice. We suggest that T-cell recruitment to the gut following SAg immune activation could be partially due to epithelium-derived chemokines.
Jedrzkiewicz
S
S
Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario, Canada.
Kataeva
G
G
Hogaboam
C M
CM
Kunkel
S L
SL
Strieter
R M
RM
McKay
D M
DM
D019819:Budesonide / Q000494:pharmacology; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D018946:Chemokine CCL5 / Q000096:biosynthesis* / Q000235:genetics; D004768:Enterotoxins / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D013211:Staphylococcus aureus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D004768:Enterotoxins; D012333:RNA, Messenger; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; C031818:enterotoxin B, staphylococcal; D019819:Budesonide; D007371:Interferon-gamma