title: Antibody-dependent reductions in mouse hookworm burden after vaccination with Ancylostoma caninum secreted protein 1.
abstract: Vaccination of mice with either third-stage Ancylostoma caninum infective hookworm larvae (L3) or alum-precipitated recombinant Ancylostoma secreted protein 1 from A. caninum (Ac-ASP-1) results in protection against hookworm challenge infections. Vaccine protection is manifested by reductions in lung hookworm burdens at 48 h postchallenge. Mice actively immunized 4 times with Ac-ASP-1 also exhibited reductions in hookworm burden in the muscles. Hookworm burden reductions from Ac-ASP-1 immunization were associated with elevations in all immunoglobulin subclasses, with the greatest rise observed in host IgG1 and IgG2b. The addition of a fourth immunization resulted in even higher levels of IgG and IgE. In contrast, L3-vaccinated mice exhibited marked elevations in IgG1 and IgM, including anti-Ac-ASP-1 IgM antibody. Passive immunization with pooled sera from recombinant Ac-ASP-1-vaccinated mice also resulted in lung hookworm burden reductions. It is hypothesized that recombinant Ac-ASP-1 vaccinations elicit antibody that interferes with parasite larval migration.
authors.affiliation: Medical Helminthology Laboratory, Department of Epidemiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA. ghosh@yale.edu
authors.forename: K
authors.initials: K
authors.lastname: Ghosh
authors.forename: P J
authors.initials: PJ
authors.lastname: Hotez
mesh_terms: D000722:Ancylostoma / Q000254:growth & development / Q000276:immunology*; D000724:Ancylostomiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D007116:Immunization, Passive; D007132:Immunoglobulin Isotypes; D008168:Lung / Q000469:parasitology; D051379:Mice; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: C099548:ASP protein, Ancylostoma caninum; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007132:Immunoglobulin Isotypes; D014614:Vaccines, Synthetic