Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6.
Defensins contribute to host defense by disrupting the cytoplasmic membrane of microorganisms. This report shows that human beta-defensins are also chemotactic for immature dendritic cells and memory T cells. Human beta-defensin was selectively chemotactic for cells stably transfected to express human CCR6, a chemokine receptor preferentially expressed by immature dendritic cells and memory T cells. The beta-defensin-induced chemotaxis was sensitive to pertussis toxin and inhibited by antibodies to CCR6. The binding of iodinated LARC, the chemokine ligand for CCR6, to CCR6-transfected cells was competitively displaced by beta-defensin. Thus, beta-defensins may promote adaptive immune responses by recruiting dendritic and T cells to the site of microbial invasion through interaction with CCR6.
Yang
D
D
Laboratory of Molecular Immunoregulation, Division of Basic Sciences, Intramural Research Support Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA.
Chertov
O
O
Bykovskaia
S N
SN
Chen
Q
Q
Buffo
M J
MJ
Shogan
J
J
Anderson
M
M
Schröder
J M
JM
Wang
J M
JM
Howard
O M
OM
Oppenheim
J J
JJ
D000906:Antibodies / Q000276:immunology; D001667:Binding, Competitive; D002460:Cell Line; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000378:metabolism / Q000494:pharmacology; D002633:Chemotaxis; D002634:Chemotaxis, Leukocyte; D023082:Defensins; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007110:Immunity, Active; D007113:Immunity, Innate; D007156:Immunologic Memory; D019402:Macrophage Inflammatory Proteins; D037342:Pertussis Toxin; D011506:Proteins / Q000494:pharmacology / Q000502:physiology*; D054399:Receptors, CCR6; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014162:Transfection; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D023083:beta-Defensins
D000906:Antibodies; C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; C106641:DEFB4A protein, human; D023082:Defensins; D019402:Macrophage Inflammatory Proteins; D011506:Proteins; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; D023083:beta-Defensins; D037342:Pertussis Toxin