Human IgM monoclonal antibody to ganglioside GM2 and complement suppress virus propagation in ex vivo cultures of lymphocytes from HIV-1 infected patients.
HIV-1 infection induces aberrant ganglioside GM2 expression on infected cell lines, and human IgM anti-GM2 monoclonal antibody (L55 Ab) together with normal fresh human serum (FHS) as a source of complement causes complement mediated cytolysis of HIV-1 infected cells as well as HIV-1 particles. We report here that high expression of GM2 was also detected on HIV-1 infected lymphocytes from HIV-1 seropositive patients. L55 Ab effectively suppressed the generation of HIV in the presence of FHS in primarily cultured lymphocytes from HIV-1 infected patients in ex vivo experiments, and the suppression was enhanced additively by AZT. These data suggest that L55 Ab may increase the therapeutic effect of chemotherapy.
Okada
N
N
Department of Molecular Biology, Nagoya City University School of Medicine, Nagoya, Japan.
Wu
X
X
Mizokami
M
M
Irie
R F
RF
Okada
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000276:immunology*; D005678:G(M2) Ganglioside / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology*; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D005678:G(M2) Ganglioside; D003165:Complement System Proteins