[Vitamins and apoptosis--induction of apoptosis in human cancer cells by nicotinic acid-related compounds].
It was found that picolinic acid, dipicolinic acid, and isonicotinamide strongly induce apoptosis in human acute myelomonocytic leukemia cells, HL-60. Cinchomeronic acid, quinolinic acid, N1-methylnicotinamide, 6-aminonicotinamide, and picolinamide were weak inducers of the apoptosis. After treatments with picolinic acid, dipicolinic acid, and isonicotinamide, apoptosis started within 4 hr and it was induced in about 50% of the cells within 8 hr. These compounds also induced apoptosis in human chronic myelogenous leukemia cells, K562 and human cervical carcinoma cells, HeLa. However, apoptosis was not induced by these three compounds in quiescent normal human lymphocytes. A wide spectrum caspase inhibitor perfectly prevented DNA fragmentation induced by these compounds. But, caspase-1 inhibitor and caspase-3 inhibitor did not block DNA fragmentation.
Taguchi
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Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University.
D017209:Apoptosis / Q000187:drug effects*; D061945:Caspase Inhibitors; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D009369:Neoplasms / Q000473:pathology*; D009536:Niacinamide / Q000494:pharmacology; D009539:Nicotinic Acids / Q000494:pharmacology*; D010848:Picolinic Acids / Q000494:pharmacology
D061945:Caspase Inhibitors; D009539:Nicotinic Acids; D010848:Picolinic Acids; D009536:Niacinamide; C027681:isonicotinamide; C030614:picolinic acid; C004860:dipicolinic acid