Induction of catecholamine synthesis in human neuroblastoma cells by replication inhibitors and sodium butyrate.
Various inhibitors of DNA synthesis induced neurite extension in human neuroblastoma cells. In order to clarify morphology-function relationship in differentiation of neuroblastoma cells, the effect of the replication inhibitors on inducibility of catecholamine synthesis was examined. The reagents alone did not affect production of dopamine and noradrenaline, but joint administration of each inhibitor and sodium butyrate considerably promoted the catecholamine synthesis, without additional change in neurite profile. Although inactive in neurite extension, sodium butyrate was moderately active in catecholamine production. The promoting effect of thymidine (or hydroxyurea) and sodium butyrate was repealed by alpha-amanitin, actinomycin D or cycloheximide.
Matsui
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T
Department of Health Information, Fukui Prefectural Institute of Public Health, Fukui, Japan. QGA01634@nifty.ne.jp
Taketo
A
A
D000546:Amanitins / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D004298:Dopamine / Q000096:biosynthesis*; D006719:Homovanillic Acid / Q000378:metabolism; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D009447:Neuroblastoma; D009638:Norepinephrine / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014642:Vanilmandelic Acid / Q000378:metabolism
D000546:Amanitins; D000970:Antineoplastic Agents; D002087:Butyrates; D003609:Dactinomycin; D014642:Vanilmandelic Acid; D003513:Cycloheximide; D000242:Cyclic AMP; D014446:Tyrosine 3-Monooxygenase; D013936:Thymidine; D004298:Dopamine; D009638:Norepinephrine; D006918:Hydroxyurea; D006719:Homovanillic Acid