title: Mutational analysis of acylphosphatase suggests the importance of topology and contact order in protein folding.
abstract: Muscle acylphosphatase (AcP) is a small protein that folds very slowly with two-state behavior. The conformational stability and the rates of folding and unfolding have been determined for a number of mutants of AcP in order to characterize the structure of the folding transition state. The results show that the transition state is an expanded version of the native protein, where most of the native interactions are partially established. The transition state of AcP turns out to be remarkably similar in structure to that of the activation domain of procarboxypeptidase A2 (ADA2h), a protein having the same overall topology but sharing only 13% sequence identity with AcP. This suggests that transition states are conserved between proteins with the same native fold. Comparison of the rates of folding of AcP and four other proteins with the same topology, including ADA2h, supports the concept that the average distance in sequence between interacting residues (that is, the contact order) is an important determinant of the rate of protein folding.
authors.affiliation: Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QT, UK.
authors.forename: F
authors.initials: F
authors.lastname: Chiti
authors.forename: N
authors.initials: N
authors.lastname: Taddei
authors.forename: P M
authors.initials: PM
authors.lastname: White
authors.forename: M
authors.initials: M
authors.lastname: Bucciantini
authors.forename: F
authors.initials: F
authors.lastname: Magherini
authors.forename: M
authors.initials: M
authors.lastname: Stefani
authors.forename: C M
authors.initials: CM
authors.lastname: Dobson
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002268:Carboxypeptidases / Q000737:chemistry; D043422:Carboxypeptidases A; D004792:Enzyme Precursors / Q000737:chemistry; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D009132:Muscles / Q000201:enzymology; D009154:Mutation / Q000235:genetics*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D020673:Protein Renaturation; D013816:Thermodynamics; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D004792:Enzyme Precursors; D000097024:Acylphosphatase