All-trans-retinoic-acid- and growth-factor- mediated induction of alkaline phosphatase activity in freshly isolated chronic myeloid leukemia cells.
Reduced or absent neutrophil alkaline phosphatase (NAP) activity is a common feature of neutrophilic granulocytes from patients with chronic myeloid leukemia (CML). In this study we examined whether NAP activity could be restored in vitro by stimulating CML cells with different promoters such as all-trans-retinoic acid (ATRA), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). The results obtained indicated that ATRA and G-CSF, either alone or in combination, were effective in inducing NAP activity in CML cells, whereas GM-CSF was not. Further, NAP restoration in ATRA- and G-CSF-treated cultures was accompanied by increased morphologic differentiation of the CML clone. It might be concluded that the CML clone could be driven in vitro by ATRA and G-CSF both to achieve granulocytic maturation and to correct functional NAP-related defects.
Stagno
F
F
Chair of Hematology, University of Catania, Italy.
Guglielmo
P
P
Consoli
U
U
Fiumara
P
P
Longo
G S
GS
Giustolisi
R
R
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000187:drug effects*; D002469:Cell Separation; D004790:Enzyme Induction; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D014212:Tretinoin; D000469:Alkaline Phosphatase