Enantiomeric separations of dansyl amino acids using the macrocyclic antibiotic A35512B as a chiral selector in capillary electrophoresis.
The macrocyclic antibiotic A35512B was examined as a chiral selector for capillary electrophoresis (CE) using thirteen racemic dansyl amino acids as test analytes. The chiral selectivity of A35512B was evaluated as a function of the run buffer pH, antibiotic concentration, and organic modifier composition. After optimizing these parameters, the macrocylic antibiotic A35512B provided high resolutions of all the enantiomers for the thirteen dansyl amino acids tested in this study.
Risley
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Eli Lilly and Company, Lilly Research Laboratories, Pharmaceutical Sciences Division, Lilly Corporate Center, Indianapolis, IN 46285, USA. risley@lilly.com
Trelli-Seifert
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McKenzie
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D000596:Amino Acids / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002021:Buffers; D003619:Dansyl Compounds / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006020:Glycopeptides; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals; D013237:Stereoisomerism
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D002021:Buffers; D003619:Dansyl Compounds; D006020:Glycopeptides; D009930:Organic Chemicals; C030783:A35512 antibiotic complex