Presence and expression of a novel variant form of ST2 gene product in human leukemic cell line UT-7/GM.
A novel variant cDNA from the human ST2 gene other than ST2 or ST2L was identified and tentatively named ST2V. Alternative splicing inserts a new exon which leads to a change in the C-terminal portion of ST2, causing it to gain a hydrophobic tail instead of losing the third immunoglobulin-like domain. ST2V is expressed in human leukemic cell line UT-7 and its sublines UT-7/GM, UT-7/EPO, and UT-7/TPO, in addition to human helper T cell line 5C10. The amount of ST2V mRNA is greatly diminished when UT-7/GM cells are induced to differentiate into either erythroblastic or megakaryoblastic phenotypes. The possible roles of the ST2V in growth and differentiation are intriguing.
Tominaga
S i
Si
Department of Medicine, Jichi Medical School, Kawachi-gun, Tochigi-ken, Japan. shintomi@jichi.ac.jp
Kuroiwa
K
K
Tago
K
K
Iwahana
H
H
Yanagisawa
K
K
Komatsu
N
N
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons; D015723:Gene Library; D014644:Genetic Variation; D015894:Genome, Human; D006801:Humans; D000072179:Interleukin-1 Receptor-Like 1 Protein; D007438:Introns; D007938:Leukemia; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D011956:Receptors, Cell Surface; D006377:T-Lymphocytes, Helper-Inducer; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C079241:IL1RL1 protein, human; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D011506:Proteins; D011956:Receptors, Cell Surface