Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems.
The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both beta-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.
Boulter
N
N
Department of Biology, University of York, UK.
Brown
D
D
Wilkie
G
G
Williamson
S
S
Kirvar
E
E
Knight
P
P
Glass
E
E
Campbell
J
J
Morzaria
S
S
Nene
V
V
Musoke
A
A
d'Oliveira
C
C
Gubbels
M J
MJ
Jongejan
F
F
Hall
R
R
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000494:pharmacology; D015800:Protozoan Proteins / Q000008:administration & dosage* / Q000494:pharmacology*; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D016796:Theileria annulata / Q000276:immunology*; D013801:Theileriasis / Q000517:prevention & control*
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; C091729:SPAG-1 antigen, Theileria annulata; D005620:Freund's Adjuvant