Effects of blood sugar level on rat transient focal brain ischemia consecutively observed by diffusion-weighted EPI and (1)H echo planar spectroscopic imaging.
The effects of blood sugar level on transient focal brain ischemia were examined by consecutive diffusion-weighted EPI and (1)H echo planar spectroscopic imaging. A remote-controlled rat intraluminal suture middle cerebral artery occlusion (MCAO) model was prepared. Animals were divided into three experimental groups: control, 1 g/kg, and 2 g/kg glucose groups (n = 6 for each). Saline or glucose was infused intraperitoneally 30 min prior to MCAO. The glucose-loaded groups showed increased lactate accumulation and marked decreases in average diffusion coefficient in the ischemic region during 40-min MCAO. These changes were correlated with blood sugar levels at the onset of MCAO. After reperfusion, all rats in the control and 1 g/kg groups recovered from the ischemic changes, but three rats with marked hyperglycemia in the 2 g/kg group showed irreversible changes. The adverse effects of hyperglycemia on transient focal brain ischemia were clearly demonstrated by sequential 2D images. Magn Reson Med 42:895-902, 1999.
Morikawa
S
S
Molecular Neuroscience Research Center, Shiga University of Medical Science, Ohtsu, Japan. morikawa@belle.shiga-med.ac.jp
Inubushi
T
T
Ishii
H
H
Nakasu
Y
Y
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D002560:Cerebrovascular Circulation; D017352:Echo-Planar Imaging; D005947:Glucose; D006943:Hyperglycemia / Q000139:chemically induced / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000175:diagnosis* / Q000503:physiopathology*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR
D001786:Blood Glucose; D019344:Lactic Acid; D005947:Glucose