title: Comparison of the expression of fibrosis-associated genes in glomeruli after renal transplantation between conventional cadaveric and non-heart-beating donors.
abstract: BACKGROUND
  The main difference between cadaveric heart-beating donors and non-heart-beating donors (NHBDs) is the degree of warm ischaemia to which the kidney is subjected. This study was designed to see if this affected the expression of fibrosis-associated genes in the early period after transplantation.
  
  
  METHODS
  A series of 29 cadaveric and 19 NHBD renal transplants was studied. Patients underwent protocol needle-core renal transplant biopsies at 1 week, 3 months and 6 months after transplantation. At least two individual glomeruli were isolated from each biopsy. Messenger RNA was extracted and genes of interest were amplified by reverse transcriptase-polymerase chain reaction, then quantified in an enzyme-linked immunosorbent assay system.
  
  
  RESULTS
  Delayed graft function was common in NHBD (17 of 19) compared with cadaveric transplants (six of 29) (P < 0.0001). Acute rejection rates were similar. The level of tissue inhibitor of metalloproteinase 1, an inhibitor of extracellular matrix degradation, was higher in kidneys from NHBDs at 1 week (P = 0.02). There were no other statistically significant differences in the expression of fibrosis-associated genes between the two groups.
  
  
  CONCLUSIONS
  Although the increased ischaemic injury in kidneys retrieved from NHBDs leads to a higher rate of delayed graft function, this does not translate into increased expression of fibrosis-associated genes after the first week.
authors.affiliation: Department of Surgery, Leicester General Hospital, UK.
authors.forename: S
authors.initials: S
authors.lastname: Jain
authors.forename: G R
authors.initials: GR
authors.lastname: Bicknell
authors.forename: S A
authors.initials: SA
authors.lastname: White
authors.forename: S T
authors.initials: ST
authors.lastname: Williams
authors.forename: P N
authors.initials: PN
authors.lastname: Furness
authors.forename: M L
authors.initials: ML
authors.lastname: Nicholson
mesh_terms: D000328:Adult; D001706:Biopsy / Q000379:methods; D002102:Cadaver; D005260:Female; D005355:Fibrosis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007674:Kidney Diseases / Q000235:genetics*; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology; D016030:Kidney Transplantation / Q000379:methods*; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D014019:Tissue Donors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta