Regulation of cyclic GMP elevation in the developing antennal lobe of the Sphinx moth, Manduca sexta.
In the moth, Manduca sexta, 3',5'-guanosine monophosphate (cGMP) is transiently elevated during adult development in about 100 neurons of the antennal lobe. We demonstrate that nearly all of these neurons are local interneurons of the lateral cluster I, that their capacity to show a strong cGMP response during development is regulated by the steroid hormone 20-hydroxyecdysone, and that in a subpopulation of these neurons cGMP elevation seems to be controlled directly by the gaseous messenger molecule nitric oxide (NO). Treatment with the acetylcholine esterase inhibitor eserine, antennal nerve transection, and electrical stimulation of the antennae suggest that NO/cGMP signaling during development is an activity-dependent process. Besides input from the antennae, input from the central brain and the ventral ganglia is involved in upregulating cGMP in the antennal-lobe neurons. Possible sources are centrifugal aminergic neurons, since application of serotonin and histamine enhances the GMP signal in local interneurons. Comparing the time course of cGMP elevation with events occurring during development leads us to the hypothesis that the NO/cGMP signaling pathway might be involved in synapse formation of a subset of antennal-lobe neurons.
Schachtner
J
J
Fachbereich Biologie, Tierphysiologie, Philipps-Universität Marburg, Karl-von-Frisch Str., 35032 Marburg, Germany.
Homberg
U
U
Truman
J W
JW
D000818:Animals; D019771:Axotomy; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D006152:Cyclic GMP / Q000032:analysis / Q000378:metabolism*; D026461:Ecdysteroids; D017952:Ganglia, Invertebrate / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D018402:Manduca / Q000254:growth & development*; D009252:NADPH Dehydrogenase / Q000032:analysis; D009474:Neurons / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000037:antagonists & inhibitors; D013256:Steroids / Q000494:pharmacology
D026461:Ecdysteroids; D020030:Nitric Oxide Donors; D013256:Steroids; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D006152:Cyclic GMP