Intra and inter-specific microsatellite variation in the Leishmania subgenus Viannia.
Leishmania species of the subgenus Viannia are responsible for a large proportion of New World leishmaniasis. Here we report the development of a set of microsatellite markers which are able to discriminate between all species within the subgenus Viannia, including the closely related species pairs: Leishmania (V.) braziliensis and Leishmania (V.) peruviana; Leishmania (V.) panamensis and Leishmania (V.) guyanensis. Potential species hybrids were uncovered in the analysis. These markers are sufficiently polymorphic such that within-species epidemiological, population and genetic studies are theoretically possible for all species analyzed.
Russell
R
R
Department of Pathology, University of Cambridge, UK.
Iribar
M P
MP
Lambson
B
B
Brewster
S
S
Blackwell
J M
JM
Dye
C
C
Ajioka
J W
JW
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007891:Leishmania / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D016054:DNA, Protozoan