PLP-I: a novel prolactin-like gene in rodents.
In this report, we describe molecular cloning and characterization of cDNAs encoding a novel rat prolactin-like protein. The rat cDNAs were isolated from the decidua and the gene was named PLP-I. cDNAs for the mouse equivalent were also cloned by the cross-hybridization technique. Pregnancy-specific expression of the rat PLP-I gene was observed in the rat placenta by Northern analysis. Location of signal peptide cleavage sites in rat and mouse pre-PLP-I proteins was predicted using a theoretical method. A molecular phylogenetic tree for the growth hormone-prolactin superfamily including the novel member, PLP-I, constructed using the neighbor-joining method, places rat/mouse PLP-I closest to rat/mouse placental lactogen I and II.
Hiraoka
Y
Y
Department of Anatomy, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan. yhfile@med.keio.ac.jp
Ogawa
M
M
Sakai
Y
Y
Takeuchi
Y
Y
Komatsu
N
N
Shiozawa
M
M
Tanabe
K
K
Aiso
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D019143:Evolution, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000235:genetics*; D011388:Prolactin / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment
D018076:DNA, Complementary; C400883:PLP-I protein, rat; D011257:Pregnancy Proteins; D011388:Prolactin