Anticonvulsant action of 2-chloroadenosine injected focally into the perirhinal cortex in amygdaloid kindled rats.
Possible anticonvulsant effects of 2-chloroadenosine injected focally into the perirhinal cortex of amygdala kindled rats were investigated over a 2 h period. Animals were microinfused (1 microl) with 2-chloroadenosine (2-CLA; 5, 10, 15, 25 and 100 nM) or artificial cerebrospinal fluid applied through a cannula located in the perirhinal cortex. At the doses employed, 2-CLA significantly reduced afterdischarge duration and stage 5 seizure duration. The latency to stage 4 seizure was increased only at the highest dose of 2-CLA (100 nM), while even at this dose no significant change in seizure stage could be seen. The maximum effect of 2-CLA was obtained 30 min after microinfusion of the drug. Pre-treatment (intraperirhinal cortex) of animals with the nonselective adenosine antagonist, caffeine (50 microM; 1 microl), blocked the anticonvulsant activity of 2-CLA. These results suggest that adenosine receptors located in the perirhinal cortex may play an important role in the suppression of seizure activity elicited from the amygdala.
Mirnajafi-Zadeh
J
J
Department of Physiology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
Pourgholami
M H
MH
Palizvan
M R
MR
Rostampour
M
M
Fallahi
M
M
D015762:2-Chloroadenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000241:Adenosine / Q000037:antagonists & inhibitors; D000679:Amygdala / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D000927:Anticonvulsants / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007267:Injections; D007696:Kindling, Neurologic; D008297:Male; D009833:Olfactory Pathways / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000503:physiopathology / Q000517:prevention & control
D000927:Anticonvulsants; D015762:2-Chloroadenosine; D002110:Caffeine; D000241:Adenosine