Lipid-induced changes in vascular smooth muscle cell membrane fluidity are associated with DNA synthesis.
In the present study, we examined whether changes in the membrane fluidity of vascular smooth muscle cells (VSMCs) alter their DNA synthesis. For this purpose, the membrane fluidity of the cells was modulated after treatment of VSMCs with 1,2-dioleoyl phosphatidylcholine (PC). Treatment of VSMCs with 1,2-dioleoyl PC-rich medium containing 10% heat-inactivated human serum and 3 mg/ ml 1,2-dioleoyl PC for 24 h resulted in an increase in VSMC membrane fluidity at all temperatures from 15 degrees to 40 degrees C as well as a 51% inhibition of DNA synthesis, compared with untreated cells. Remarkably, enrichment of VSMCs with 1,2-dioleoyl PC/cholesterol-rich medium containing 10% human serum, 3 mg/ml 1,2-dioleoyl PC and 2 mg/ml cholesterol restored both membrane fluidity and DNA synthesis to the levels of untreated cells. The present findings show an inverse association between increased membrane fluidity and cellular DNA synthesis.
Sachinidis
A
A
Medizinische Universitäts-Poliklinik, Bonn, Germany. sachinidis@uni-bonn.de
Carniel
M
M
Seewald
S
S
Seul
C
C
Gouni-Berthold
I
I
Ko
Y
Y
Vetter
H
H
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY; D013816:Thermodynamics
D003470:Culture Media; D010713:Phosphatidylcholines; D004247:DNA; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine