Determination of LD-Ig complex with counter immunoelectrophoresis using terylene cellulose acetate membrane as supporting media.
OBJECTIVE
In order to determine abnormal LD isoenzyme bands in serum, we established a method of counter immunoelectrophoresis using terylene cellulose acetate as supporting media.


METHODS
Department of Clinical Laboratory, Southwestern Hospital, Chongqing 400038 China.


METHODS
LD isoenzymes were electrophoretically fractionated with a Paragon electrophoretic system. LD anomaly was identified by counter immunoelectrophoresis using terylene cellulose acetate as supporting media.


METHODS
Identification of the LD-Ig complex using a simple and practical method.


RESULTS
An abnormal LD-4 band and an extra band on the cathodic side of LD-5 were observed in the serum of a post-burn patient and proved to be an LD-IgG complex.


CONCLUSIONS
We have established a useful and efficient protocol that uses both isoenzyme electrophoresis and counter immunoelectrophoresis to identify abnormal isoenzyme bands. The method of counter immunoelectrophoresis we describe, using terylene cellulose acetate as supporting media, is simple and suitable for routine clinical use.
Liu
Z J
ZJ
Clinical Laboratory, Southwestern Hospital, Chongqing, People's Republic of China.
Yang
X
X
D000936:Antigen-Antibody Complex / Q000097:blood*; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000276:immunology; D008567:Membranes, Artificial
D000936:Antigen-Antibody Complex; D001672:Biocompatible Materials; D007074:Immunoglobulin G; D007527:Isoenzymes; D008567:Membranes, Artificial; D002482:Cellulose; D007770:L-Lactate Dehydrogenase