Identification of the cDNA encoding human 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway(1).
We report the sequence of a human cDNA encoding a protein homologous to devB (a bacterial gene often found in proximity to the gene encoding glucose-6-phosphate dehydrogenase in bacterial genomes) and to the C-terminal part of human hexose-6-phosphate dehydrogenase. The protein was expressed in Escherichia coli, purified and shown to be 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway. Sequence analysis indicates that bacterial devB proteins, the C-terminal part of hexose-6-phosphate dehydrogenase and yeast Sol1-4 proteins are most likely also 6-phosphogluconolactonases and that these proteins are related to glucosamine-6-phosphate isomerases.
Collard
F
F
Laboratory of Physiological Chemistry, Université catholique de Louvain and Christian de Duve Institute of Cellular Pathology, 7539 Avenue Hippocrate, B-1200, Brussels, Belgium.
Collet
J F
JF
Gerin
I
I
Veiga-da-Cunha
M
M
Van Schaftingen
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D002384:Catalysis; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010427:Pentose Phosphate Pathway / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase