title: Control of Leishmania infantum infection is associated with CD8(+) and gamma interferon- and interleukin-5-producing CD4(+) antigen-specific T cells.
abstract: Visceral leishmaniasis is a severe and lethal disease caused by the protozoan parasites of the genus Leishmania. In areas where leishmaniasis is endemic, most infected individuals control the infection and remain asymptomatic; chemotherapy of visceral leishmaniasis restores some immunity which protects against relapses. In the present study, Leishmania-specific T-cell clones were established from six asymptomatic and five cured patients. Cytokines production by these clones was analyzed. A large fraction of the parasite-specific T-cell clones from asymptomatic patients were CD8(+) and produced high amounts of gamma interferon (IFN-gamma). Most CD4(+) T-cell clones from two asymptomatic subjects exhibited an unusual phenotype: production of high levels of IFN-gamma low levels of interleukin-4, (IL-4), but high levels of IL-5. In contrast, only few parasite-specific CD8(+) T-cell clones were obtained from cured patients after chemotherapy; moreover, CD4(+) T-cell clones from these patients exhibited an heterogeneous profile of cytokines from Th1-like to Th2-like phenotypes. These results point to CD8(+) T cells and to IL-5- and IFN-gamma-producing CD4(+) T cells as possible contributors to human resistance to Leishmania infection. They should stimulate new immunological approaches in the control of this disease.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U 399, Laboratoire de Parasitologie, Faculté de Médecine, Université de la Méditerranée, Marseille, France. charles.mary@medecine.univ-mrs.fr
authors.forename: C
authors.initials: C
authors.lastname: Mary
authors.forename: V
authors.initials: V
authors.lastname: Auriault
authors.forename: B
authors.initials: B
authors.lastname: Faugère
authors.forename: A J
authors.initials: AJ
authors.lastname: Dessein
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D015848:Interleukin-5 / Q000502:physiology*; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000276:immunology*; D008297:Male
chemical_list: D015848:Interleukin-5; D007371:Interferon-gamma