Neuroprotective effect of high-dose albumin therapy against global ischemic brain injury in rats.
The purpose of this study was to determine whether treatment with high-dose human serum albumin (HSA) would offer protection in a model of high-grade transient forebrain ischemia. Twenty-six fasted Wistar rats underwent bilateral common carotid artery occlusion and severe hypotension (50 mmHg) for 10 min. The agent (25% HSA) or vehicle (0.9% NaCl) was administered i.v. 5 min after termination of ischemia. HSA-treated rats showed significantly improved neurological deficits throughout a 7-day survival period. Histologically, HSA-treated rats showed 2.4- to 5.3-fold increases in numbers of surviving CA1 hippocampal pyramidal neurons compared to saline-treated animals. These results document that high-dose albumin therapy instituted 5 min after global ischemia significantly improves neurological score and reduces histological damage.
Belayev
L
L
Cerebral Vascular Disease Research Center, Department of Neurology (D4-5), University of Miami School of Medicine, P.O. Box 016960, Miami, FL 33101, USA. lbelayev@stroke.med.miami.edu
Saul
I
I
Huh
P W
PW
Finotti
N
N
Zhao
W
W
Busto
R
R
Ginsberg
M D
MD
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000098:blood supply / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000188:drug therapy / Q000473:pathology
D000418:Albumins; D018696:Neuroprotective Agents