Infiltration of lymphocytes in the limbic brain following stimulation of subclinical cellular immunity and low dosages of lithium and a cholinergic agent.
This experiment was designed to investigate the hypothesis that single small dosages of lithium (1.5 mEq/kg), the muscarinic agent pilocarpine (15 mg/kg) and spinal cord emulsion encourage perivascular infiltration of lymphocytes into the brain even when overt symptoms of experimental allergic encephalomyelitis are not apparent. The brains of rats that had received this small dosage of lithium and pilocarpine exhibited discernable infiltrations of lymphocytes within limbic tracts but no discernable neuronal loss. Although the brains of the rats that displayed overt seizures following larger dosages of lithium (3 mEq/kg) and pilocarpine (30 mg/kg) exhibited the usual pattern of neuronal loss within multiple thalamic and limbic structures and conspicuous foci of lymphocytic infiltration (particularly within the hippocampal formation) the correlation between the numbers of foci and the proportions of neuronal damage in these structures was not significant statistically. These results indicate that infiltrations of lymphocytes into brain parenchyma are not simple artifacts of the amount of neuronal damage and may be sensitive toxicological markers for subclinical interactions between drugs and immune responses.
Cook
L L
LL
Neuroscience Laboratory, Laurentian University, Sudbury, Ont., Canada.
Persinger
M A
MA
D000818:Animals; D018678:Cholinergic Agents / Q000633:toxicity*; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects / Q000502:physiology*; D008032:Limbic System / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D008094:Lithium / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D010277:Parasympathomimetics / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D012640:Seizures / Q000139:chemically induced / Q000473:pathology
D018678:Cholinergic Agents; D010277:Parasympathomimetics; D010862:Pilocarpine; D008094:Lithium