title: Clinical significance of genetic imbalances revealed by comparative genomic hybridization in chondrosarcomas.
abstract: DNA copy number changes were studied by comparative genomic hybridization (CGH) in 50 chondrosarcoma samples from 45 patients. Mean number of genetic aberrations in primary tumors was 4.8 +/- 1.8. The most frequently gained regions were 20q12-qter (37%), 20q (32%), 8q24.1-qter (27%), 20p (24%), and 14q24-qter (24%). Losses were 5.5 times less frequent than gains and observed mainly at Xcen-q21, 6cen-q22, and 18cen-q11.2 (11% each). Recurrent and metastatic tumors showed a mean of 4.0 +/- 2.2 aberrations per sample. The most frequently gained regions were chromosome 7 (4 cases), 5q14-q32 (4 cases), 6p (3 cases), and 12q (3 cases). Losses of DNA sequences were 3.4 times less frequent than gains. Histological tumor grade was significantly associated with metastasis-free survival (P = .002) and overall survival (P = .003), being the strongest prognostic factor tested. A statistically significant correlation was found between gain at 8q24.1-qter and shorter overall survival (P = .01) but not with local recurrence or metastasis-free survival. Gain at 14q24-qter was associated with a trend to shorter overall survival (P = .05) but neither with an increased risk for local recurrence nor with metastasis-free survival. In a multivariate analysis, only the tumor grade associated with overall survival (P = .02). In a multivariate analysis together with the tumor grade, gain at 8q24.1-qter did not retain its significance (P = .44), indicating that this imbalance is not an independent prognostic factor.
authors.affiliation: Department of Medical Genetics, Haartman Institute, University of Helsinki, Finland.
authors.forename: M L
authors.initials: ML
authors.lastname: Larramendy
authors.forename: N
authors.initials: N
authors.lastname: Mandahl
authors.forename: F
authors.initials: F
authors.lastname: Mertens
authors.forename: C
authors.initials: C
authors.lastname: Blomqvist
authors.forename: A H
authors.initials: AH
authors.lastname: Kivioja
authors.forename: E
authors.initials: E
authors.lastname: Karaharju
authors.forename: J
authors.initials: J
authors.lastname: Valle
authors.forename: T
authors.initials: T
authors.lastname: Böhling
authors.forename: M
authors.initials: M
authors.lastname: Tarkkanen
authors.forename: A
authors.initials: A
authors.lastname: Rydholm
authors.forename: M
authors.initials: M
authors.lastname: Akerman
authors.forename: H C
authors.initials: HC
authors.lastname: Bauer
authors.forename: J P
authors.initials: JP
authors.lastname: Anttila
authors.forename: I
authors.initials: I
authors.lastname: Elomaa
authors.forename: S
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002813:Chondrosarcoma / Q000235:genetics* / Q000401:mortality / Q000556:secondary; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011003:Ploidies; D015996:Survival Rate