title: Biomonitoring of occupational exposure to styrene in a plastics lamination plant.
abstract: A comprehensive approach to biological monitoring of 44 workers occupationally exposed to styrene in a hand lamination plant was performed by using several end-points: styrene in workplace air, styrene in exhaled air, styrene in blood, DNA strand breaks (SBs) and oxidised bases in mononuclear leukocytes, chromosomal aberrations in lymphocytes, immune parameters and genotyping of polymorphic genes of some xenobiotic-metabolizing enzymes (CYP 1A1, EPHX, GSTM1 and GSTP1). We found a significantly higher number of DNA SBs, measured by a modified comet assay, in mononuclear leukocytes of the styrene-exposed workers compared with results from 19 unexposed controls (P<0.001). A fairly strong correlation was observed between SBs and years of exposure (P<0.001, r=0.545). The styrene-exposed workers also showed a significantly increased frequency of chromosomal aberrations (P<0.0001 for highly exposed group, P<0.004 for medium-exposed group, and P=0.0001 for low-exposed group). The proliferative response of T-lymphocytes stimulated with concanavalin A was significantly suppressed in people exposed to styrene (P<0.05). We recorded a significant increase of the percentage of monocytes in differential white blood cell counts in the exposed group (P<0.05). Using flow cytometry, we found an increased expression of adhesion molecules CD62L, CD18, CD11a, CD11b, CD49d and CD54 in the exposed workers as compared with the control group (P<0.05).
authors.affiliation: Institute of Preventive and Clinical Medicine, Limbová 14, 833 01, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Somorovská
authors.forename: E
authors.initials: E
authors.lastname: Jahnová
authors.forename: J
authors.initials: J
authors.lastname: Tulinská
authors.forename: M
authors.initials: M
authors.lastname: Zámecníková
authors.forename: J
authors.initials: J
authors.lastname: Sarmanová
authors.forename: A
authors.initials: A
authors.lastname: Terenová
authors.forename: L
authors.initials: L
authors.lastname: Vodicková
authors.forename: A
authors.initials: A
authors.lastname: Lísková
authors.forename: B
authors.initials: B
authors.lastname: Vallová
authors.forename: P
authors.initials: P
authors.lastname: Soucek
authors.forename: K
authors.initials: K
authors.lastname: Hemminki
authors.forename: H
authors.initials: H
authors.lastname: Norppa
authors.forename: A
authors.initials: A
authors.lastname: Hirvonen
authors.forename: A D
authors.initials: AD
authors.lastname: Tates
authors.forename: L
authors.initials: L
authors.lastname: Fuortes
authors.forename: M
authors.initials: M
authors.lastname: Dusinská
authors.forename: P
authors.initials: P
authors.lastname: Vodicka
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000633:toxicity; D015415:Biomarkers; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002869:Chromosome Aberrations; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D016273:Occupational Exposure; D010969:Plastics; D020058:Styrene / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D004798:Enzymes; D010969:Plastics; D020058:Styrene