Cloning of a cDNA encoding ovine keratinocyte growth factor.
A cDNA encoding the ovine keratinocyte growth factor (KGF) has been cloned. A 622-bp cDNA, containing the entire protein coding region, was amplified from an ovine adenocarcinoma fibroblast cell line. The cDNA was found to share 95% nucleotide identity with dog KGF, 94% identity with human KGF, 90% identity with mouse KGF and 88% identity with rat KGF. The predicted ovine amino-acid sequence shares 98.5, 98, 96 and 94% identity, respectively, with the corresponding dog, human, mouse and rat proteins. The KGF gene is present as a single copy in the ovine genome.
Mitchell
J E
JE
Moredun Research Institute, Edinburgh, UK.
McInnes
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C496453:FGF7 protein, human; C496454:Fgf7 protein, mouse; C496455:Fgf7 protein, rat; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors