title: Requirement for Ras/Rac1-mediated p38 and c-Jun N-terminal kinase signaling in Stat3 transcriptional activity induced by the Src oncoprotein.
abstract: Signal transducers and activators of transcription (STATs) are transcription factors that mediate normal biologic responses to cytokines and growth factors. However, abnormal activation of certain STAT family members, including Stat3, is increasingly associated with oncogenesis. In fibroblasts expressing the Src oncoprotein, activation of Stat3 induces specific gene expression and is required for cell transformation. Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. We investigated the signaling pathways underlying the constitutive Stat3 activation in Src oncogenesis. Expression of Ras or Rac1 dominant negative protein blocks Stat3-mediated gene regulation induced by Src in a manner consistent with dependence on p38 and c-Jun N-terminal kinase (JNK). Both of these serine/threonine kinases and Stat3 serine phosphorylation are constitutively induced in Src-transformed fibroblasts. Furthermore, inhibition of p38 and JNK activities suppresses constitutive Stat3 serine phosphorylation and Stat3-mediated gene regulation. In vitro kinase assays with purified full-length Stat3 as the substrate show that both JNK and p38 can phosphorylate Stat3 on serine. Moreover, inhibition of p38 activity and thus of Stat3 serine phosphorylation results in suppression of transformation by v-Src but not v-Ras, consistent with a requirement for Stat3 serine phosphorylation in Src transformation. Our results demonstrate that Ras- and Rac1-mediated p38 and JNK signals are required for Stat3 transcriptional activity induced by the Src oncoprotein. These findings delineate a network of tyrosine and serine/threonine kinase signaling pathways that converge on Stat3 in the context of oncogenesis.
authors.affiliation: Molecular Oncology, Moffitt Cancer Center, University of South Florida College of Medicine, Tampa, Florida 33612, USA.
authors.forename: J
authors.initials: J
authors.lastname: Turkson
authors.forename: T
authors.initials: T
authors.lastname: Bowman
authors.forename: J
authors.initials: J
authors.lastname: Adnane
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: J Y
authors.initials: JY
authors.lastname: Djeu
authors.forename: M
authors.initials: M
authors.lastname: Sekharam
authors.forename: D A
authors.initials: DA
authors.lastname: Frank
authors.forename: L B
authors.initials: LB
authors.lastname: Holzman
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Sebti
authors.forename: R
authors.initials: R
authors.lastname: Jove
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D048031:JNK Mitogen-Activated Protein Kinases; D020935:MAP Kinase Signaling System; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008957:Models, Genetic; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D010766:Phosphorylation; D050796:STAT3 Transcription Factor; D029701:Saccharomyces cerevisiae Proteins; D012694:Serine / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D050796:STAT3 Transcription Factor; D029701:Saccharomyces cerevisiae Proteins; C494087:Stat3 protein, mouse; D015534:Trans-Activators; D012694:Serine; D015688:Oncogene Protein pp60(v-src); C088589:HOG1 protein, S cerevisiae; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D020830:rac1 GTP-Binding Protein