Thermal features of the bovine serum albumin unfolding by polyethylene glycols.
Albumin showed very poor affinity for polyethylene glycol molecular weight (Mw) 1000 (30 M(-1)) and Mw 8000 (400 M(-1)) (PEG 1000 and PEG 8000). Polyethylene glycol of low Mw favours the ionization of the tyrosine (TYR) residues of albumin. Such variation might be a consequence of the change in dielectric constant at the domain of the protein by PEG binding. PEGs of high Mws stabilize the native compact state of human albumin showing negative preferential interaction with the protein. Interaction between PEGs and albumin is thermodynamically unfavourable, and becomes even more unfavourable for denatured proteins whose surface areas are larger than those of native ones leading to a stabilization of the unfolded state, which is manifested as a lowering of the thermal transition temperature. PEG 8000 perturbs the structure of the protein surface, partially modifying the layer of water and the microenvironment of the superficial aromatic residues (tryptophan, TRP and TYR) which is in agreement with the modifications of the UV spectrum of albumin by PEG 8000 and circular dichroism (CD) spectrum at high temperatures.
Farruggia
B
B
Department of Chemical Physics and CONICET, Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Argentina.
Nerli
B
B
Di Nuci
H
H
Rigatusso
R
R
Picó
G
G
D020106:Acrylamide / Q000737:chemistry; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002942:Circular Dichroism; D005227:Fatty Acids / Q000378:metabolism; D005453:Fluorescence; D007700:Kinetics; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014443:Tyrosine / Q000378:metabolism; D014783:Viscosity
D000817:Anilino Naphthalenesulfonates; D005227:Fatty Acids; D020106:Acrylamide; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D014443:Tyrosine; C027132:1-anilino-8-naphthalenesulfonate; D001663:Bilirubin