Regulation of mouse somatostatin receptor type 2 gene expression by glucocorticoids.
Somatostatin is a regulatory peptide with important inhibitory functions. Its effects are mediated by five receptors, sst1-sst5. Previous studies revealed that sst2 contains three alternative, tissue specific promoters. Here, molecular mechanisms for the regulation of sst2 transcription by glucocorticoids were investigated. Reporter gene assays in NG 108-15 cells and electrophoretic mobility shift assays revealed that a glucocorticoid responsive element at position -1044 on the second promoter mediates dexamethasone induction. These findings, the existence of a cAMP response element and the tissue dependent activity (brain, pituitary and gastrointestinal tissues) indicate the importance of the second sst2 gene promoter.
Kraus
J
J
Department of Pharmacology and Toxicology, University of Magdeburg, Leipzigerstrasse 44, D-39120, Magdeburg, Germany. Juergen.Kraus@medizin.uni-magdeburg.de
Wöltje
M
M
Höllt
V
V
D000818:Animals; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000502:physiology*; D051379:Mice; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000235:genetics*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D017481:Receptors, Somatostatin; C093733:somatostatin receptor 2