Molecular cytogenetic resources specific for chromosome 12.
We have generated a panel of 20 somatic cell hybrids retaining fragments of human chromosome 12. Each hybrid was characterized cytogenetically by reverse fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged sites (STSs) spaced evenly along the chromosome. The panel can be exploited to map subregionally DNA sequences on chromosome 12 and to generate partial chromosome paints useful in the characterization of chromosomal rearrangements involving this chromosome. Furthermore, a panel of 58 yeast artificial chromosomes (YACs) mapping to chromosome 12 was characterized by FISH experiments on normal human metaphases. A subset of this panel is recognized by the STSs used in the somatic cell hybrid characterization. In this way a correlation between the genetic and the physical maps of this chromosome can be established.
Viggiano
L
L
Institute of Genetics, University of Bari, Bari, Italy.
Miolla
V
V
Ricco
A
A
Storlazzi
C T
CT
Pennacchia
M
M
Fratello
A
A
Mancini
M
M
Marzella
R
R
Archidiacono
N
N
Rocchi
M
M
D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D006224:Cricetinae; D020732:Cytogenetic Analysis; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D016324:Sequence Tagged Sites