Molecular cloning of mXCR1, the murine SCM-1/lymphotactin receptor.
Single C motif-1 (SCM-1)/lymphotactin is a C-type member of the chemokine superfamily. Previously, we identified its specific receptor XCR1. Here we isolated the murine homologue of XCR1 (mXCR1). To demonstrate its biological activity, we produced recombinant mouse SCM-1 by the baculovirus expression system. B300-19 murine pre-B cells expressing mXCR1 responded to mSCM-1 in chemotactic and calcium-mobilization assays. mXCR1 mRNA was weakly expressed in spleen and lung of normal C57BL/6 mice. In spleen, CD8+ cells and NK1.1+ cells were found to express mXCR1. Identification of mXCR1 will now allow us to study the role of this unique cytokine system in the mouse models of inflammation and immunity.
Yoshida
T
T
Shionogi Institute for Medical Science, Osaka, Japan.
Izawa
D
D
Nakayama
T
T
Nakahara
K
K
Kakizaki
M
M
Imai
T
T
Suzuki
R
R
Miyasaka
M
M
Yoshie
O
O
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002458:Cell Fractionation; D002460:Cell Line; D002633:Chemotaxis; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000502:physiology*; D019707:Receptors, Chemokine / Q000235:genetics* / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D019707:Receptors, Chemokine; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins; C551408:XC chemokine receptor 1, mouse; C091671:XCR1 protein, human; D002118:Calcium