Overexpression of CD11b on eosinophils in atopic dermatitis: downregulation by cyclosporin A and upregulation by interleukin 5.
We examined the level of expression of CD11b on eosinophils in pripheral blood samples from patients with atopic dermatitis (AD) and non-AD volunteers. Eosinophils were defined using a new method employing CD14/CD45 and a backgate technique. Overexpression of CD11b was noted in eosinophils of AD patients. Treatment of AD with cyclosporin A resulted in clinical improvement as well as reduction in the expression of CD11b. Stimulation of eosinophils from patients with inactive AD by interleukin 5 upregulated the expression of CD11b on these cells. Our results suggest that the expression of CD11b surface molecule on eosinophils may play an important role in the activity of AD.
Yamada
H
H
Department of Dermatology, School of Medicine, Kinki University, Osaka, Japan. yamadahi@med.kindai.ac.jp
Kurashimo
S
S
Chihara
J
J
Matsukura
M
M
Yudate
T
T
Tezuka
T
T
D016572:Cyclosporine / Q000494:pharmacology*; D003876:Dermatitis, Atopic / Q000097:blood* / Q000276:immunology; D015536:Down-Regulation / Q000187:drug effects; D004804:Eosinophils / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects
D007166:Immunosuppressive Agents; D015848:Interleukin-5; D016177:Macrophage-1 Antigen; D016572:Cyclosporine