The cartilage-derived, C-type lectin (CLECSF1): structure of the gene and chromosomal location.
Cartilage is a tissue that is primarily extracellular matrix, the bulk of which consists of proteoglycan aggregates constrained within a collagen framework. Candidate components that organize the extracellular assembly of the matrix consist of collagens, proteoglycans and multimeric glycoproteins. We describe the human gene structure of a potential organizing factor, a cartilage-derived member of the C-type lectin superfamily (CLECSF1; C-type lectin superfamily) related to the serum protein, tetranectin. We show by Northern analysis that this protein is restricted to cartilage and locate the gene on chromosome 16q23. We have characterized 10.9 kb of sequence upstream of the first exon. Similarly to human tetranectin, there are three exons. The residues that are conserved between CLECSF1 and tetranectin suggest that the cartilage-derived protein forms a trimeric structure similar to that of tetranectin, with three N-terminal alpha-helical domains aggregating through hydrophobic faces. The globular, C-terminal domain that has been shown to bind carbohydrate in some members of the family and plasminogen in tetranectin, is likely to have a similar overall structure to that of tetranectin.
Neame
P J
PJ
Shriners Hospital for Children, Tampa, FL 33612, USA. pneame@com1.med.usf.edu
Tapp
H
H
Grimm
D R
DR
D000595:Amino Acid Sequence; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics; D002874:Chromosome Mapping; D003094:Collagen / Q000378:metabolism*; D005091:Exons; D015723:Gene Library; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D037102:Lectins / Q000235:genetics*; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D001798:Blood Proteins; C122077:CLEC3A protein, human; D006023:Glycoproteins; D037102:Lectins; D037181:Lectins, C-Type; C048648:tetranectin; D003094:Collagen