Effect of ethanol on the activity and conformation of Penaeus penicillatus acid phosphatase.
The effect of ethanol on the activity of Penaeus penicillatus acid phosphatase has been studied. The results show that ethanol significantly inhibits enzyme activity as a non-competitive inhibitor, with Ki 8.75%. The conformational changes of the enzyme molecule induced by ethanol were followed using fluorescence emission, ultraviolet difference and circular dichroism (CD) spectra. Increasing the ethanol concentration caused the fluorescence emission intensity of the enzyme to increase. The ultraviolet difference spectra of the enzyme denatured with ethanol had two negative peaks at 220 and 278 nm, and a positive peak at 240 nm. Increasing the ethanol concentration produced a small shoulder peak at 287 nm in addition to the increases in the negative magnitudes of the 220 and 278 nm peaks. The changes of the fluorescence and ultraviolet difference spectra reflected the changes of the microenvironments of the tryptophan and tyrosine residues of the enzyme. The CD spectrum changes of the enzyme show that the secondary structure of the enzyme also changed. The results suggest that ethanol is a non-competitive inhibitor and the conformational integrity of the enzyme is essential for its activity.
Chen
Q X
QX
Department of Biology, Xiamen University, People's Republic of China.
Zhang
R Q
RQ
Yang
P Z
PZ
Li
Y
Y
Chen
S L
SL
Li
S
S
Yang
Y
Y
Zhou
H M
HM
D000135:Acid Phosphatase / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002942:Circular Dichroism; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D007700:Kinetics; D033561:Penaeidae / Q000201:enzymology*; D011487:Protein Conformation; D012996:Solutions
D012996:Solutions; D000431:Ethanol; D000135:Acid Phosphatase