The expression of the pathogenic yeast Candida albicans catalase gene in response to hydrogen peroxide.
The catalase gene of the pathogenic yeast Candida albicans was cloned and its expression was examined. Activity of the catalase was detected when cells which were in the early logarithmic stage were treated with hydrogen peroxide. Additionally, activity was detected without any treatment to cells in the late logarithmic and stationary phases. When cells were cultured in galactose, glycerol, or ethanol, catalase activity was always observed without the hydrogen peroxide treatment, suggesting that glucose represses the induction of catalase expression. To elucidate the molecular mechanism of catalase expression, the putative gene for catalase and its 5' untranscribed region were cloned. Sequences of the gene and its potential regulatory region revealed several motifs, including a GC box-like element and stress-responsive element (STRE), which could be involved in the transcriptional regulation. Northern analysis showed that hydrogen peroxide and sorbitol activated transcription of the catalase. On the other hand, treatment of glucose strictly repressed the expression of the catalase even when co-treated with hydrogen peroxide. The expression of catalase against treatment with hydrogen peroxide took place very quickly and decreased slowly in the experimental condition adopted here. From these results, we assumed that the expression of the catalase in Candida albicans is regulated by various environmental conditions via motifs for transcriptional activation as in other yeast catalases.
Nakagawa
Y
Y
Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Aichi, Japan.
Koide
K
K
Watanabe
K
K
Morita
Y
Y
Mizuguchi
I
I
Akashi
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004790:Enzyme Induction; D015870:Gene Expression; D005800:Genes, Fungal; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data
D004271:DNA, Fungal; D006861:Hydrogen Peroxide; D002374:Catalase