Effect of electromagnetic fields on several CD markers and transcription and expression of CD4.
We carried out flow cytometric analysis for multiparametric evaluation of cell surface markers related to cellular functions. Specifically, we studied the expression of CD4, CD8, CD3, CD16, CD19, HLA-DR, and CD14 macrophage receptors expression and cell cycle progression on cells exposed to ELF-EMF. In addition, we tested the effects of ELF-EMF on CD4 mRNA protein transcription and translation and the cell-cycle progression using an immunofluorescence method. Our data show that same CD surface marker expression are weakly influenced by electromagnetic fields, with no differences between cells exposed or not exposed to ELF-EMFs. However, when the CD4 protein generation was studied, an indication of protein production was found in lymphocytes exposed to ELF-EMF, as evidenced by immunofluorescence, Western blotting and RT-PCR analysis. CD16 and CD14 expression were affected by EMF exposure at all times studied (24, 48, 72 h). The results obtained with cell cycle analysis show that after 48 h of exposure to ELF-EMF, PHA-activated and not activated cells in S phase increase with respect to non-exposed cells. The findings from this study demonstrate that under our defined experimental conditions there is evidence that ELF-EMF has a slight effect on CD4, CD14 and CD16 receptor expression, while the other CD receptors are not affected.
Conti
P
P
Department of Oncology and Neurosciences, University of Chieti, Medical School, Italy. pconti@unich.it
Reale
M
M
Grilli
A
A
Barbacane
R C
RC
Di Luzio
S
S
Di Gioacchino
M
M
De Lutiis
M A
MA
Felaco
M
M
D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D015415:Biomarkers; D015153:Blotting, Western; D017252:CD3 Complex / Q000096:biosynthesis; D015704:CD4 Antigens / Q000235:genetics*; D016827:CD8 Antigens / Q000096:biosynthesis; D002453:Cell Cycle; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology; D012333:RNA, Messenger; D017452:Receptors, IgG / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D015703:Antigens, CD; D015415:Biomarkers; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D017452:Receptors, IgG