A chemiluminescent catecholamine assay: its application for monitoring adrenergic transmitter release.
A chemiluminescent procedure for measuring catecholamines (dopamine, norepinephrine, epinephrine) is described. It is based on the observation that lactoperoxidase catalyses both the oxidation of catecholamines, and the chemiluminescent reaction of luminol with their oxidation product. The assay has been adapted for continuously monitoring the release of catecholamines from adrenergic tissues, from cell suspensions and from cells loaded in culture with dopamine.
Israël
M
M
Laboratoire de Neurobiologie, Cellulaire et Moléculaire, C.N.R.S., Gif-Sur-Yvette, France.
Tomasi
M
M
D000311:Adrenal Glands / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002395:Catecholamines / Q000032:analysis* / Q000378:metabolism; D018929:Cell Culture Techniques; D004298:Dopamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008163:Luminescent Measurements; D051379:Mice; D017072:Neostriatum / Q000737:chemistry / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D009924:Organ Culture Techniques
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine