title: Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe.
abstract: We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV-1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.
authors.affiliation: Department of Immunology, University of Zimbabwe, Harare. lzijenah@healthnet.zw
authors.forename: L S
authors.initials: LS
authors.lastname: Zijenah
authors.forename: J
authors.initials: J
authors.lastname: Humphrey
authors.forename: K
authors.initials: K
authors.lastname: Nathoo
authors.forename: L
authors.initials: L
authors.lastname: Malaba
authors.forename: P
authors.initials: P
authors.lastname: Zvandasara
authors.forename: A
authors.initials: A
authors.lastname: Mahomva
authors.forename: P
authors.initials: P
authors.lastname: Iliff
authors.forename: M T
authors.initials: MT
authors.lastname: Mbizvo
mesh_terms: D000328:Adult; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D015658:HIV Infections / Q000175:diagnosis; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D049590:Postpartum Period; D011247:Pregnancy; D012680:Sensitivity and Specificity; D015030:Zimbabwe
chemical_list: D017931:DNA Primers; D004279:DNA, Viral