title: Oligomerization of the EK18 mutant of the trp repressor of Escherichia coli as observed by NMR spectroscopy.
abstract: The regulation of the trp repressor system of Escherichia coli is frequently modeled by a single equilibrium, that between the aporepressor (TR) and the corepressor, l-tryptophan (Trp), at their intracellular concentrations. The actual mechanism, which is much more complex and more finely tuned, involves multiple equilibria: TR and Trp association, TR oligomerization, specific and nonspecific binding of various states of TR to DNA, and interactions between these various species and ions. TR in isolation exists primarily as a homodimer, but the state of oligomerization increases as the TR concentration goes up and/or the salt concentration goes down, leading to species with lower affinity for DNA. We have used multinuclear, multidimensional NMR spectroscopy to investigate structural changes that accompany the oligomerization of TR. For these investigations, the superrepressor mutant EK18 (TR with Glu 18 replaced by Lys) was chosen because it exhibits less severe oligomerization at higher protein concentration than other known variants; this made it possible to study the dimer to tetramer oligomerization step by NMR. The NMR results suggest that the interaction between TR dimers is structurally linked to folding of the DNA binding domain and that it likely involves direct contacts between the C-terminal residues of the C-helix of one dimer with the next dimer. This implies that oligomerization can compete with DNA binding and thus serves as a factor in the fine-tuning of gene expression.
authors.affiliation: Department of Biochemistry and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Y K
authors.initials: YK
authors.lastname: Chae
authors.forename: F
authors.initials: F
authors.lastname: Abildgaard
authors.forename: C A
authors.initials: CA
authors.lastname: Royer
authors.forename: J L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D019943:Amino Acid Substitution; D001059:Apoproteins / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism*; D018698:Glutamic Acid; D008239:Lysine; D046911:Macromolecular Substances; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018698:Glutamic Acid; D008239:Lysine; D046911:Macromolecular Substances; D012097:Repressor Proteins; C027190:TRPR protein, E coli; D029968:Escherichia coli Proteins