Identification and expression of a cDNA for human hydroxypyruvate/glyoxylate reductase.
The isolation and expression of a human liver cDNA encoding a 40-kDa protein with glyoxylate and hydroxypyruvate reductase activities is described. The cDNA (GLXR) is 1235 bp and consists of a predicted open reading frame of 987 bp with a 225-bp 3'-untranslated region. The 328-amino acid protein has partial sequence similarity to hydroxypyruvate and glyoxylate reductases from a variety of plant and microbial species.
Rumsby
G
G
Department of Molecular Pathology, University College London, Windeyer Institute of Medical Sciences, UK. g.rumsby@academic.uclh.nthames.nhs.uk
Cregeen
D P
DP
D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002237:Carbohydrate Dehydrogenases / Q000235:genetics; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D050537:Hydroxypyruvate Reductase; D006960:Hyperoxaluria, Primary / Q000201:enzymology / Q000235:genetics; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D016415:Sequence Alignment; D000637:Transaminases / Q000378:metabolism; D014162:Transfection
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C020830:glyoxylate reductase; C020669:Glycerate dehydrogenase; D050537:Hydroxypyruvate Reductase; C020821:glycollate oxidase; D000637:Transaminases; C019635:Alanine-glyoxylate transaminase