Molecular markers reveal exclusively clonal reproduction in Trichophyton rubrum.
Genotypic variability among 96 Trichophyton rubrum strains which displayed different colony morphologies and were collected from four continents was investigated. Twelve markers representing 57 loci were analyzed by PCR fingerprinting, amplified fragment length polymorphism, and random amplified monomorphic DNA markers. Interestingly, none of the methods used revealed any DNA polymorphism, indicating a strictly clonal mode of reproduction and a strong adaptation to human skin.
Gräser
Y
Y
Department of Microbiology and Hygiene (Charité-Virchow), Humboldt University, Berlin, Germany. yvonne.graeser@charite.de
Kühnisch
J
J
Presber
W
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014005:Tinea / Q000453:epidemiology / Q000382:microbiology; D014249:Trichophyton / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification
D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers