title: A new subtilisin-like proteinase from roots of the dandelion Taraxacum officinale Webb S. L.
abstract: A serine proteinase from roots of Taraxacum officinale Webb S. L. was isolated by affinity chromatography and gel-filtration on Superose 6R using FPLC. The enzyme is a 67-kD glycoprotein containing 54% carbohydrate which we have named taraxalisin. The substrate specificity of taraxalisin toward synthetic peptides and oxidized insulin B-chain is comparable with that of cucumisin from Cucumis melo and the subtilisin-like serine proteinase macluralisin from Maclura pomifera. The proteinase is inactivated by DFP and PMSF. Taraxalisin exhibits maximal activity at pH 8.0. The pH range for stability of the enzyme is narrow--6.0-9.0. The temperature optimum for the subtilisin-like activity is 40 degrees C. The N-terminal sequence of taraxalisin has 40% of its residues identical to those of subtilisin Carlsberg. Thus, the serine proteinase from dandelion roots is a member of the subtilisin family, which is evidently widespread in the plant kingdom.
authors.affiliation: Department of Natural Compounds, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. annamb@genebee.msu.su
authors.forename: A M
authors.initials: AM
authors.lastname: Bogacheva
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaya
authors.forename: A
authors.initials: A
authors.lastname: Preusser
authors.forename: I O
authors.initials: IO
authors.lastname: Tchikileva
authors.forename: Y E
authors.initials: YE
authors.lastname: Dunaevsky
authors.forename: B N
authors.initials: BN
authors.lastname: Golovkin
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D007328:Insulin / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000201:enzymology; D010944:Plants / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D007328:Insulin; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; C115700:taraxalisin