title: Binding of bovine IgG2a and IgG2b allotypes to protein A, protein G, and Haemophilus somnus IgBPs.
abstract: Immunoglobulin binding proteins (IgBPs) are thought to be virulence factors which enable pathogens to evade the host's immune response. Since bovine IgG2 is important in protection against pyogenic infections, the binding characteristics of Staphylococcus aureus protein A (PrA), streptococcal protein G (PrG), or Haemophilus somnus high molecular weight IgBPs to the two bovine IgG2 allotypes were examined. For PrA or PrG binding of IgG2, guinea pig red blood cells coated with specific IgG2a or IgG2b antibodies were used in a competitive binding inhibition assay with unlabeled and horseradish peroxidase-labeled PrA or PrG. To determine which sizes of H. somnus. IgBPs bind to the two IgG2 allotypes, immunoblots with H. somnus culture supernatant were probed with anti-DNP IgG2a and IgG2b. This detects only Fc binding because anti-DNP does not cross-react with H. somnus antigens. Both IgG2 allotypes bound equally well to PrA and PrG. However, IgG2b but not IgG2a bound to H. somnus high molecular weight IgBPs. The lack of differential binding of bovine IgG2 allotypes to PrA and PrG means that these IgBPs can be considered to be unbiased reagents in assays for detection of bovine IgG2 or for immunoaffinity purification. The differential binding of H. somnus IgBPs to the IgG2 allotypes indicates that animals having one allotype may be more resistant to H. somnus infection than animals having the other allotype.
authors.affiliation: University of California San Diego, School of Medicine, UCSD Medical Center CTF-C415, 92103-8416, USA.
authors.forename: F D
authors.initials: FD
authors.lastname: Bastida-Corcuera
authors.forename: K H
authors.initials: KH
authors.lastname: Nielsen
authors.forename: L B
authors.initials: LB
authors.lastname: Corbeil
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002352:Carrier Proteins / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D002848:Chromatography, DEAE-Cellulose; D006190:Haemophilus / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A