title: Chitin synthase 2 gene sequence of Malassezia species.
abstract: Nucleotide sequences of the chitin synthase 2 (CHS2) gene of seven species, Malassezia furfur, M. globosa, M. obtusa, M. pachydermatis, M. restricta, M. slooffiae and M. sympodialis, were analyzed for their phylogenetic relationship. About 620-bp genomic DNA fragments of the CHS2 gene were amplified from these Malassezia species by polymerase chain reaction (PCR) and sequenced. The CHS2 nucleotide sequences of these Malassezia species showed more than 95% similarity between the species. A phylogenetic analysis of the nucleotide sequences of CHS2 gene fragments of seven Malassezia species revealed that the species were genetically distinct from each other.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kano
authors.forename: T
authors.initials: T
authors.lastname: Aizawa
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D001483:Base Sequence; D002687:Chitin Synthase / Q000145:classification / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D008290:Malassezia / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004271:DNA, Fungal; D002687:Chitin Synthase; C400821:chitin synthase 2