Transglycosylation of cellobiose by partially purified Trichoderma viride cellulase.
A commercial cellulase from Trichoderma viride was fractionated into three fractions, F1, F2, and F3, in order to investigate transglycosylation activities. Among these fractions, F3, which demonstrated highly hydrolytic activity toward p-nitrophenyl beta-D-glucopyranoside and Avicel, most effectively catalyzed the transglycosylation of cellobiose and converted cellobiose into beta-Glc-(1-->6)-beta-glc-(1-->4)-Glc and beta-Glc-(1-->6)-beta-Glc-(1-->6)-beta-Glc(1-->4)-Glc. The F3 fraction contained the enzyme to catalyze beta-glucosyl transfer toward only the C-6 position of the sugar acceptor, and thus it is expected to be of use for syntheses of functional oligosaccharides.
Kono
H
H
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan. kohno@dove-mc.eng.hokudai.ac.jp
Waelchli
M R
MR
Fujiwara
M
M
Erata
T
T
Takai
M
M
D002240:Carbohydrate Sequence; D002475:Cellobiose / Q000737:chemistry*; D002480:Cellulase / Q000302:isolation & purification*; D005591:Chemical Fractionation; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*
D009844:Oligosaccharides; D002475:Cellobiose; D002480:Cellulase