Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in cultured Glycyrrhiza echinata cells treated with yeast extract.
Yeast extract-treated suspension cultures of a new cell line, AK-1, of Glycyrrhiza echinata were induced to produce an isoflavonoid phytoalexin (medicarpin) and metabolites of retrochalcone/flavone pathway (echinatin, licodione, and 7,4'-dihydroxyflavone). From these cells, putative full-length cDNAs encoding cytochrome P450s, (2S)-flavanone 2-hydroxylase and isoflavone 2'-hydroxylase, were cloned.
Nakamura
K
K
Department of Applied Biological Sciences, Nihon University, Kanagawa, Japan.
Akashi
T
T
Aoki
T
T
Kawaguchi
K
K
Ayabe
S
S
D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D006035:Glycyrrhiza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics; D010946:Plants, Medicinal; D015003:Yeasts / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D005419:Flavonoids; D007529:Isoflavones; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C115412:cytochrome P-450 CYP81E1 (Glycyrrhiza echinata); C114032:cytochrome P-450 CYP93B1 (Glycyrrhiza echinata)