Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants.
Vertebrate mechanosensory hair cells contain a narrow "pericuticular" zone which is densely populated with small vesicles between the cuticular plate and cellular junctions near the apical surface. The presence of many cytoplasmic vesicles suggests that the apical surface of hair cells has a high turnover rate. The significance of intense membrane trafficking at the apical surface is not known. Using a marker of endocytosis, the styryl dye FM1-43, this report shows that rapid apical endocytosis in zebrafish lateral line sensory hair cells is calcium and calmodulin dependent and is partially blocked by the presence of amiloride and dihydrostreptomycin, known inhibitors of mechanotransduction channels. As seen in lateral line hair cells, sensory hair cells within the larval otic capsule also exhibit rapid apical endocytosis. Defects in internalization of the dye in both lateral line and inner ear hair cells were found in five zebrafish auditory/vestibular mutants: sputnik, mariner, orbiter, mercury, and skylab. In addition, lateral line hair cells in these mutants were not sensitive to prolonged exposure to streptomycin, which is toxic to hair cells. The presence of endocytic defects in the majority of zebrafish mechanosensory mutants points to a important role of apical endocytosis in hair cell function.
Seiler
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Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, Abteilung Biochemie, 72076 Tübingen, Germany.
Nicolson
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D000165:Acridine Orange / Q000032:analysis; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000502:physiology*; D004232:Diuretics / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects / Q000235:genetics*; D005456:Fluorescent Dyes / Q000032:analysis; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008465:Mechanoreceptors / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011726:Pyridinium Compounds / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012235:Rhodamines / Q000032:analysis; D013307:Streptomycin / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology / Q000502:physiology
D002147:Calmodulin; D004232:Diuretics; D018492:Dopamine Antagonists; C073804:FM1 43; D005456:Fluorescent Dyes; D011500:Protein Synthesis Inhibitors; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D012235:Rhodamines; D014268:Trifluoperazine; D000584:Amiloride; D000165:Acridine Orange; D002118:Calcium; D013307:Streptomycin