Adhesion molecules in patients after lung transplantation.
Leukocyte adhesion molecules, such as intercellular adhesion molecule (ICAM)-1 and its ligands, are involved in inflammatory processes of the lung. For ICAM-1, differential expression during different kinds of complications after transplantation has been proposed. We analyzed the role of ICAM-1, CD18, CD11a, CD11b, and CD11c during episodes of rejection or infection in patients after lung transplantation and compared the results to episodes without apparent complication. A total of 98 bronchoalveolar lavage (BAL) samples and 90 serum samples were analyzed. ICAM-1, CD18, CD11a, CD11b, and CD11c expressions were detected immunocytochemically on alveolar macrophages. Soluble ICAM-1 was quantified in serum and BAL. In the control group, 49.8 +/- 18% of macrophages stained positive for CD11b. During rejection, the mean of cells showing CD11b on the surface was significantly higher (64.6 +/-11.4%) with no difference compared to episodes of infection (59.7 +/-22.7). All other epitopes were not expressed differently with regard to a normal clinical course or episodes of infection and rejections. In summary, assessment of ICAM-1 and corresponding ligands did not allow for a reliable discrimination between episodes of rejection or infection in lung transplantation.
Bewig
B
B
Department of Internal Medicine, Christian-Albrechts-University, Kiel, Germany. bbewig@1med.uni-kiel.de
Tiroke
A
A
Böttcher
H
H
Padel
K
K
Hirt
S
S
Haverich
A
A
Cremer
J
J
D000293:Adolescent; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D018845:CD11 Antigens / Q000032:analysis* / Q000097:blood; D018821:CD18 Antigens / Q000032:analysis* / Q000097:blood; D006084:Graft Rejection / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007239:Infections / Q000175:diagnosis / Q000378:metabolism; D016023:Integrins / Q000032:analysis / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000097:blood; D016040:Lung Transplantation; D016676:Macrophages, Alveolar / Q000737:chemistry; D008875:Middle Aged
D018845:CD11 Antigens; D018821:CD18 Antigens; D016023:Integrins; D018799:Intercellular Adhesion Molecule-1