Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout.
A method was developed for determining benzocaine and N-acetylbenzocaine concentrations in fillet tissue of rainbow trout. The method involves extracting the analytes with acetonitrile, removing lipids or hydrophobic compounds from the extract with hexane, and providing additional clean-up with solid-phase extraction techniques. Analyte concentrations are determined using reversed-phase high-performance liquid chromatographic techniques with an isocratic mobile phase and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., < 14%), and sensitivity (method quantitation limit, < 24 ng/g) for each analyte indicate the usefulness of this method for studies characterizing the depletion of benzocaine residues from fish exposed to benzocaine.
Meinertz
J R
JR
US Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, WI 54603, USA.
Stehly
G R
GR
Hubert
T D
TD
Bernardy
J A
JA
D000818:Animals; D001566:Benzocaine / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D005504:Food Analysis; D017686:Oncorhynchus mykiss; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001566:Benzocaine