Biphasic change in correlation between ovarian lipid peroxides and progestational activity during pseudopregnancy induced in immature rats.
We measured ovarian lipid peroxide (LP) levels and plasma progestins, progesterone (P4) and 20alpha-dihydroprogesterone, throughout pseudopregnancy in gonadotropin-primed immature rats. Plasma P4 fluctuated, with two peaks on days 5 (PSP5) and 8 of pseudopregnancy, and then declined to the basal level by PSP12. Ovarian LP increased from PSP1 to PSP4, decreased temporarily until PSP8, and then rose gradually until PSP14. From PSP1 through PSP7, ovarian LP was positively correlated with total progestins according to the Spearman ranked correlation coefficient (r=+0.829, p<0.05). In contrast, a negative correlation between ovarian LP and plasma P4 was apparent (r=-0.816, p<0.05) from PSP8 to PSP14. These results show the biphasic correlation of LP with luteal progestational activity depending on the luteal stage.
Kurusu
S
S
Laboratory of Veterinary Physiology, Kitasato University School of Veterinary Medicine and Animal Sciences, Towada, Aomori, Japan.
Tsukamoto
K
K
Konishi
H
H
Tachibana
M
M
Kawaminami
M
M
Hashimoto
I
I
D004092:20-alpha-Dihydroprogesterone / Q000097:blood; D000818:Animals; D006063:Chorionic Gonadotropin / Q000502:physiology; D005260:Female; D008054:Lipid Peroxides / Q000032:analysis / Q000502:physiology*; D008183:Luteal Phase / Q000502:physiology; D008315:Malondialdehyde / Q000032:analysis; D010053:Ovary / Q000502:physiology*; D010060:Ovulation; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011372:Progestins / Q000097:blood*; D011555:Pseudopregnancy / Q000503:physiopathology / Q000662:veterinary*; D011863:Radioimmunoassay / Q000662:veterinary; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
D006063:Chorionic Gonadotropin; D008054:Lipid Peroxides; D011372:Progestins; D004092:20-alpha-Dihydroprogesterone; D011374:Progesterone; D008315:Malondialdehyde