Structural studies of the O-specific polysaccharide of Hafnia alvei strain PCM 1207 lipopolysaccharide.
The structure of the O-specific side-chain of the Hafnia alvei strain PCM 1207 lipopolysaccharide (LPS) has been investigated. Methylation analysis, partial acid hydrolysis, matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) MS, fast atom bombardment (FAB)-MS/MS and 1H- and 13C-NMR spectroscopy were the principal methods used. Glycerol phosphate was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was established: The polysaccharide is partially (approximately 10%) substituted with O-acetyl groups. The lipopolysaccharide was also subjected to high resolution magic angle spinning (HR-MAS) NMR analysis, which showed both the signals of the O-specific polysaccharide as well as several signals from unsubstituted core oligosaccharides. This confirmed the presence of the described structure in the native LPS.
Jachymek
W
W
Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Czaja
J
J
Niedziela
T
T
Lugowski
C
C
Kenne
L
L
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002954:Citrobacter / Q000276:immunology; D003429:Cross Reactions; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000276:immunology; D016056:Immunodominant Epitopes / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D016056:Immunodominant Epitopes; D008070:Lipopolysaccharides; D019081:O Antigens; C402599:PCM 1207 lipopolysaccharide