Isolation of two human cDNAs, HLP3 and HLP4, homologous to the neuron-specific calcium-binding protein genes.
We have cloned and sequenced two types of human cDNA, HLP3 and HLP4, encoding a protein of 191 amino acid residues with four EF-hand calcium-binding motifs. The HLP3 and HLP4 proteins are homologous to the neuron-specific calcium-binding proteins and are likely human counterparts of the neural visinin-like protein(NVP) 1 and NVP2 identified in the rat brain (Kajimoto et al. 1993), displaying 98% and 99% amino acid identities with these sequences, respectively. The human HLP3 and 4 mRNAs are detected only in the brain and found at high amount in the cerebral cortex and cerebellum by Northern blot analysis.
Kobayashi
M
M
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Sakai
E
E
Furuta
Y
Y
Takamatsu
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D020832:EF Hand Motifs / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051596:Neurocalcin; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D044169:Receptors, Calcium-Sensing; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C496687:NVP2 protein, rat; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D012333:RNA, Messenger; D044169:Receptors, Calcium-Sensing; C496688:VSNL1 protein, human; C496690:Vsnl1 protein, rat