title: High-performance liquid chromatographic determination of peroxisomicine A1 (T-514) in genus Karwinskia.
abstract: A chromatographic method was developed for the T-514 determination in Karwinskia leaves, stems and roots. A C18 analytical column and a mobile phase consisting of methanol and McIlvaine buffer (pH 3) were used. T-514 was detected using a diode array detector and the chromatograms were recorded at 269 and 410 nm. A linear dependence of a peak area on the T-514 concentration (r=0.9991) was obtained in the range of 0.126-12.6 microg/ml. Limits of T-514 quantification (signal-to-noise ratio 10) in plant samples were 126 ng/ml at 410 nm and 28 ng/ml at 269 nm. T-514 was extracted from the plant material with ethyl acetate. Optimal extraction conditions were studied: number of extraction steps, volume of extracting agent and extraction time. The extracts were cleaned up using solid-phase extraction (SPE). SPE recoveries of 99.9% and 98.4% were achieved for the T-514 concentrations of 1.4 microg/ml and 0.26 microg/ml, respectively.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak Technical University, Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Bovanová
authors.forename: E
authors.initials: E
authors.lastname: Brandsteterová
authors.forename: A
authors.initials: A
authors.lastname: Caniová
authors.forename: K
authors.initials: K
authors.lastname: Argalásová
authors.forename: A
authors.initials: A
authors.lastname: Lux
mesh_terms: D000873:Anthracenes / Q000032:analysis*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D010947:Plants, Toxic; D031955:Rhamnus / Q000737:chemistry*
chemical_list: D000873:Anthracenes; D000972:Antineoplastic Agents, Phytogenic; C075149:Karwinskia toxin T-514