Function for p300 and not CBP in the apoptotic response to DNA damage.
The cellular response to ionizing radiation (IR) includes the induction of apoptosis. The p300/CBP proteins possess histone acetyltransferase activity and function as transcriptional coactivators of p53. We have prepared cells deficient in p300 or CBP to define the roles of these proteins in the cellular response to DNA damage. The present results demonstrate that p300, but not CBP, contributes to IR sensitivity of cells. The results also demonstrate that IR-induced apoptosis is impaired in the p300-, but not CBP-, deficient cells. These findings indicate that p300 functions in the apoptotic response to DNA damage.
Yuan
Z M
ZM
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, MA 02115, USA.
Huang
Y
Y
Ishiko
T
T
Nakada
S
S
Utsugisawa
T
T
Shioya
H
H
Utsugisawa
Y
Y
Shi
Y
Y
Weichselbaum
R
R
Kufe
D
D
D000230:Adenocarcinoma / Q000473:pathology; D017209:Apoptosis / Q000502:physiology*; D001943:Breast Neoplasms / Q000473:pathology; D050882:CREB-Binding Protein; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D005260:Female; D016193:G1 Phase / Q000528:radiation effects; D006801:Humans; D009687:Nuclear Proteins / Q000172:deficiency / Q000502:physiology*; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000172:deficiency / Q000502:physiology*; D015533:Transcriptional Activation; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014410:Tumor Stem Cell Assay
D009687:Nuclear Proteins; D016337:RNA, Catalytic; D015534:Trans-Activators; D004247:DNA; D050882:CREB-Binding Protein; C494363:CREBBP protein, human