title: N-linked glycans containing linear poly-N-acetyllactosamine as sorting signals in endocytosis in Trypanosoma brucei.
abstract: African trypanosomes, such as Trypanosoma brucei, are protozoan parasites that are transmitted by the tsetse fly and cause sleeping sickness in humans and Nagana in cattle. Trypanosomes evade the immune responses of their hosts by varying their surface coat protein (VSG) and restricting exocytosis and endocytosis to an invagination of the plasma membrane called the flagellar pocket (FP). The FP represents only 0.5% of the cellular surface but membrane turnover here occurs at high rates [1] [2] [3]. No model has yet been proposed to account for the sequestration of membrane proteins and the rate of membrane turnover that occur in the FP. Recent data have suggested that glycans are involved in the sorting of membrane proteins in polarized cells [4] [5] [6] [7]. Here, we show that N-linked glycans containing linear poly-N-acetyllactosamine (pNAL) are only associated with proteins of the FP/endocytic pathway in T. brucei and are present only in bloodstream forms of the parasite. These glycoproteins bind to tomato lectin (TL), a property that allowed their single-step isolation. Chito-oligosaccharides that compete specifically for pNAL binding to TL also inhibited receptor-mediated uptake of several ligands. These results suggest a model in which N-linked linear pNAL acts as a sorting signal for endocytosis in trypanosomes.
authors.affiliation: Laboratory of Molecular Parasitology, ULB - Institute of Molecular Biology and Medicine, 12 Rue des Professeurs Jeener et Brachet, B-6041,Gosselies, Belgium. dnolan@alize.ulb.ac.be.
authors.forename: D P
authors.initials: DP
authors.lastname: Nolan
authors.forename: M
authors.initials: M
authors.lastname: Geuskens
authors.forename: E
authors.initials: E
authors.lastname: Pays
mesh_terms: D000818:Animals; D004705:Endocytosis; D006801:Humans; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*; D015398:Signal Transduction; D014346:Trypanosoma brucei brucei / Q000276:immunology* / Q000502:physiology*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000737:chemistry / Q000276:immunology
chemical_list: D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D014643:Variant Surface Glycoproteins, Trypanosoma; C034981:tomato lectin; C037199:poly-N-acetyllactosamine