title: Microtubule polyglutamylation in Drosophila melanogaster brain and testis.
abstract: The presence of glutamylated tubulin, a widespread posttranslational modification of alpha- and beta-tubulin, has been investigated in Drosophila melanogaster using the specific monoclonal antibody GT335. We show here that this modification is strongly detected in brain and testis whereas other tissues analyzed did not appear to contain any glutamylated isoforms. Neuronal microtubules are glutamylated on alpha-tubulin only whereas sperm flagella showed a strong modification of both alpha- and beta-tubulin. These results argue for an essential role for glutamylation in differentiation processes that require microtubule stabilization.
authors.affiliation: Laboratoire de Physiologie Cellulaire des Invertébrés, Université Paris VI, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Bobinnec
authors.forename: C
authors.initials: C
authors.lastname: Marcaillou
authors.forename: A
authors.initials: A
authors.lastname: Debec
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D005407:Flagella / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008297:Male; D008870:Microtubules / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D014404:Tubulin; D011099:Polyglutamic Acid