title: CR3, FcgammaRIIA and FcgammaRIIIB induce activation of the respiratory burst in human neutrophils: the role of intracellular Ca(2+), phospholipase D and tyrosine phosphorylation.
abstract: Human neutrophils express two different types of phagocytic receptors, complement receptors (CR) and Fc receptors. In order to characterize the different signaling properties of each receptor we have used non-adherent human neutrophils and investigated CR3, FcgammaRIIA and FcgammaRIIIB for their signaling capacity. Selective activation of each receptor was achieved by coupling specific antibodies to heat-killed Staphylococcus aureus particles, Pansorbins, through their Fc moiety. Despite the fact that these particles are not phagocytosed, we show that addition of Pansorbins with anti-CD18 antibodies recognizing CR3 induced prominent signals leading to a respiratory burst. Stimulation with anti-FcgammaRIIIB Pansorbins induced about half of the response induced by anti-CR3 Pansorbins, whereas anti-FcgammaRIIA Pansorbins induced an even weaker signal. However, FcgammaRIIA induced strong phosphorylation of p72(syk) whereas FcgammaRIIIB induced only a very weak p72(syk) phosphorylation. During CR3 stimulation no tyrosine phosphorylation of p72(syk) was seen. Both phospholipase D and NADPH oxidase activities were dependent on intracellular calcium. This is in contrast to tyrosine phosphorylation of p72(syk) that occurred even in calcium-depleted cells, indicating that oxygen metabolism does not affect p72(syk) phosphorylation. Inhibitors of tyrosine phosphorylation blocked the respiratory burst induced by both FcgammaRIIA and FcgammaRIIIB as well as CR3. This shows that tyrosine phosphorylation of p72(syk) is an early signal in the cascade induced by FcgammaRIIA but not by CR3.
authors.affiliation: Department of Biomedicine and Surgery, Division of Cellbiology, University of Linköping, S-581 85, Linköping, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Löfgren
authors.forename: L
authors.initials: L
authors.lastname: Serrander
authors.forename: M
authors.initials: M
authors.lastname: Forsberg
authors.forename: A
authors.initials: A
authors.lastname: Wilsson
authors.forename: A
authors.initials: A
authors.lastname: Wasteson
authors.forename: O
authors.initials: O
authors.lastname: Stendahl
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004789:Enzyme Activation; D006801:Humans; D016177:Macrophage-1 Antigen / Q000276:immunology / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism; D010766:Phosphorylation; D011092:Polyethylene Glycols; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism*; D016897:Respiratory Burst; D015398:Signal Transduction; D013205:Staphylococcal Protein A; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D016177:Macrophage-1 Antigen; D017452:Receptors, IgG; D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols; D014443:Tyrosine; D019255:NADPH Oxidases; D010739:Phospholipase D; D002118:Calcium