title: Increased interleukin-1 (IL-1) and imbalance between IL-1 and IL-1 receptor antagonist during acute inflammation in experimental Shigellosis.
abstract: Infection by the enteric bacterial pathogen Shigella results in intense mucosal inflammation and destruction of the colonic and rectal epithelium in infected humans. Initial bacterial translocation occurs through the follicle-associated epithelium. Previous experiments suggest that interleukin-1 (IL-1) is crucial to trigger inflammation, particularly in the follicular zones. During the first 4 hours of infection in a rabbit ligated-loop model of intestinal invasion, there are two salient characteristics: (i) a high concentration of IL-1alpha and IL-1beta, both in infected Peyer's patch tissue and in the corresponding efferent mesenteric blood, and (ii) a very low level of expression of IL-1 receptor antagonist (IL-1ra). These may reflect a combination of regulation of expression and secretion of IL-1alpha, IL-1beta, and IL-1ra by both resident and recruited phagocytes and the induction of mononuclear phagocyte apoptosis by Shigella. This low IL-1ra/IL-1 ratio likely accounts for the rapid, uncontrolled inflammation characteristic of shigellosis.
authors.affiliation: Unité de Pathogénie Microbienne Moléculaire/Unité INSERM 389, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: J
authors.initials: J
authors.lastname: Arondel
authors.forename: M
authors.initials: M
authors.lastname: Singer
authors.forename: A
authors.initials: A
authors.lastname: Matsukawa
authors.forename: A
authors.initials: A
authors.lastname: Zychlinsky
authors.forename: P J
authors.initials: PJ
authors.lastname: Sansonetti
mesh_terms: D000208:Acute Disease; D000818:Animals; D017209:Apoptosis; D004405:Dysentery, Bacillary / Q000276:immunology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology*; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000032:analysis; D008264:Macrophages / Q000473:pathology; D008297:Male; D010581:Peyer's Patches / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D012795:Sialoglycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D012795:Sialoglycoproteins