Isolation, cloning, and expression of a new murine zinc finger encoding gene.
With the aim of identifying genes involved in cartilage differentiation, we have used a subtractive hybridization strategy with cDNAs from a chondrocytic cell line (MC615) and mRNAs from a mesenchymal precursor cell line (10T1/2). We have isolated a cDNA clone representing a novel mouse gene. The predicted 368-amino acid protein, designated ZF-12, contains four C(2)H(2)-type zinc finger motifs and one region homologous to the LeR domain, a finger-associated structural domain. ZF-12 mRNAs are expressed during embryonic development and in different organs in adult, including rib cartilage. These data suggest that ZF-12 might play an important role not only in cartilage differentiation, but also in basic cellular processes.
Prost
J F
JF
Institut de Biologie et Chimie des Protéines, CNRS UPR 412, 7 passage du Vercors 69367, Lyon, France.
Nègre
D
D
Cornet-Javaux
F
F
Cortay
J C
JC
Cozzone
A J
AJ
Herbage
D
D
Mallein-Gerin
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D016335:Zinc Fingers / Q000235:genetics*