Analysis of beta-N-oxalyl-L-alpha,beta-diaminopropionic acid and homoarginine in Lathyrus sativus by capillary zone electrophoresis.
A simple capillary zone electrophoresis (CZE) method has been developed for the simultaneous quantitative determination of beta-N-oxalyl-L-alpha,beta-diaminopropionic acid (beta-ODAP) and homoarginine in Lathyrus sativus (LS; grass pea). A new Na2B4O7-Na2SO4 run buffer was used and the pH was 9.20, contents of beta-ODAP and homoarginine in crude extracts of LS plant material were determined with this method, the RSDs of peak areas of beta-ODAP and homoarginine were 2.62% and 3.61%, respectively. It was found that the equilibrium concentration ratio of alpha- and beta-ODAP decreased from 34.5/65.5 to 28.6/71.4 when the pH of the solution increased from pH 3.0 to pH 11.0.
Zhao
L
L
Department of Chemistry, Lanzhou University, China.
Chen
X
X
Hu
Z
Z
Li
Q
Q
Chen
Q
Q
Li
Z
Z
D000599:Amino Acids, Diamino / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D006709:Homoarginine / Q000032:analysis*; D010946:Plants, Medicinal; D013056:Spectrophotometry, Ultraviolet
D000599:Amino Acids, Diamino; D006709:Homoarginine; C100126:oxalyldiaminopropionic acid