title: Leukocyte adhesion molecules and leukocyte-platelet interactions during hemodialysis: effects of different synthetic membranes.
abstract: Membranes made from synthetic polymers, in general, are considered as being biocompatible membranes and tend to be treated as a homogeneous group. However, all of these membranes have multiple and different characteristics that may contribute to interactions with blood components. As a consequence, the biocompatibility profile of synthetic membranes may vary. In the present cross-over study, we examined by flow cytometry the effects (expressed as % change from predialysis values) of three different synthetic polymers (polysulfone, PSF; polyacrylonitrile-co-sodium methallyl sulfonate, AN69; ethylenevinylalcohol, EVAL) on the expression of leukocyte adhesion molecules (CD11b/CD18, CD15s) and the interactions between leukocytes and platelets under conditions of routine clinical use. For neutrophils, a statistically significant difference was found in CD15s expression for EVAL as compared to AN69 (p<0.05) and in CD11b/CD18 expression for PSF as compared to both EVAL (p<0.01) and AN69 (p<0.05). No difference between membranes was found on the expression of such adhesive molecules on monocytes. Significant differences in platelet-neutrophil (but not in platelet-monocyte) coaggregate formation were observed between PSF and both EVAL (p<0.001) and AN69 (p<0.01). Reactive oxygen species production by neutrophil population during hemodialysis was significantly different between each pair of synthetic polymers (PSF vs EVAL, p<0.001; PSF vs AN69, p<0.001; AN69 vs EVAL, p<0.05). Our data demonstrate that in terms of leukocyte adhesion receptors and platelet-leukocyte interactions, the biocompatibility profile of the synthetic membranes polysulphone, AN69 and EVAL shows many similarities but also several significant differences. Our results support the concept that biocompatibility evaluation of each membrane should be based exclusively on data generated by that membrane in order to avoid errors based on assumptions about group characteristics.
authors.affiliation: Institute of Nephrology, Department of Medicine, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Sirolli
authors.forename: E
authors.initials: E
authors.lastname: Ballone
authors.forename: L
authors.initials: L
authors.lastname: Amoroso
authors.forename: L
authors.initials: L
authors.lastname: Di Liberato
authors.forename: R
authors.initials: R
authors.lastname: Di Mascio
authors.forename: P
authors.initials: P
authors.lastname: Capelli
authors.forename: A
authors.initials: A
authors.lastname: Albertazzi
authors.forename: M
authors.initials: M
authors.lastname: Bonomini
mesh_terms: D000180:Acrylic Resins; D000368:Aged; D000704:Analysis of Variance; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism*; D018845:CD11 Antigens / Q000032:analysis; D018821:CD18 Antigens / Q000032:analysis; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002450:Cell Communication / Q000502:physiology; D018592:Cross-Over Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007676:Kidney Failure, Chronic / Q000628:therapy; D007962:Leukocytes / Q000502:physiology; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D008875:Middle Aged; D011108:Polymers; D011145:Polyvinyls; D017382:Reactive Oxygen Species / Q000378:metabolism; D006435:Renal Dialysis / Q000295:instrumentation* / Q000379:methods; D013450:Sulfones
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D018845:CD11 Antigens; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016256:Lewis X Antigen; D008567:Membranes, Artificial; D011108:Polymers; D011145:Polyvinyls; D017382:Reactive Oxygen Species; D013450:Sulfones; C010504:polyacrylonitrile; C020320:ethylene-vinyl alcohol copolymer; C017662:polysulfone P 1700