title: Identification and developmental expression of Ci-msxb: a novel homologue of Drosophila msh gene in Ciona intestinalis.
abstract: We report the cloning and expression pattern of Ci-msxb the second Ciona intestinalis homeobox gene homologue to the Drosophila muscle segment homeobox (msh) gene. Northern blot analysis showed that transcripts appeared at gastrula stage, peaked in the early tailbud and decreased during the tailed stages. Whole mount in situ hybridization showed that the Ci-msxb expression first is detected at 110 cell-stage in the blastomeres that are precursors of different tissue (muscle, spinal cord, endodermal strand, brain, mesenchyme, pigmented cells and primordial pharynx). Transcript level declined in mesoderm cells after the completion of gastrulation, but mRNAs were still present in the folding neural plate during neurulation and in the pigmented cells. Later, at larval stage, transcripts were present around the otolith and ocellus, in a restricted part of the nervous system and in the primordial pharynx; the gene expression was conserved after metamorphosis in the juvenile.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Stazione Zoologica 'A. Dohrn', Naples, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Aniello
authors.forename: A
authors.initials: A
authors.lastname: Locascio
authors.forename: M G
authors.initials: MG
authors.lastname: Villani
authors.forename: A
authors.initials: A
authors.lastname: Di Gregorio
authors.forename: L
authors.initials: L
authors.lastname: Fucci
authors.forename: M
authors.initials: M
authors.lastname: Branno
mesh_terms: D000818:Animals; D001757:Blastomeres; D002938:Ciona intestinalis / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D004475:Ectoderm; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics; D007814:Larva; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology / Q000254:growth & development; D017386:Sequence Homology, Amino Acid
chemical_list: C096010:Dr protein, Drosophila; D029721:Drosophila Proteins; D018398:Homeodomain Proteins