title: Why do we determine alpha-glucosidase activity in human semen during infertility work-up?
abstract: alpha-Glucosidase is a normal constituent of human semen, produced mainly in the epididymis. It is significantly correlated to sperm count. Its activity is low in cases of epididymal obstruction. We evaluated alpha-glucosidase activity in 653 semen samples of patients, who attended our department for marital infertility, with respect to associations to clinical and other seminal parameters. The normal range (mean +/- 2 SD) in samples with normal parameter values was 7.2-46.4 mU ml-1. The determination in patients with azoospermia revealed mean values of 7.7 +/- 9.5 mU ml-1 in obstructive azoospermia, and 15.8 +/- 11.5 mU ml-1 in nonobstructive azoospermia. The difference was not statistically significant in that the sensitivity of determination with respect to the presence of obstruction was only 0.66, and the specificity 0.83. A significant correlation (r = 0.34) of alpha-glucosidase activity with log sperm count was observed. The mean alpha-glucosidase activity was not significantly different in groups formed according to sperm motility, according to leucocyte count or according to semen volume. A difference between smokers and nonsmokers with comparable sperm count, as reported in the literature, did not occur. We conclude from our results that the determination of alpha-glucosidase activity does not give additional information of the fertility status exceeding that of other clinical investigations or parameters of semen analysis.
authors.affiliation: Department of Andrology, School of Medicine, Philipps University, Marburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Krause
authors.forename: C
authors.initials: C
authors.lastname: Bohring
mesh_terms: D006801:Humans; D008297:Male; D009845:Oligospermia / Q000201:enzymology*; D012661:Semen / Q000201:enzymology*; D013076:Sperm Count; D013081:Sperm Motility; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D000520:alpha-Glucosidases