Evaluation of the cytocompatibility of three endodontic materials.
The goal of this in vitro study was to evaluate the relative cytocompatibility of three endodontic materials: calcium hydroxide, a calcium oxide-based compound, and a zinc oxide-eugenol-based sealer. The evaluation was conducted 24, 72, and 168 h after contact with the compounds and involved three complementary techniques: a colorimetric cytotoxicity test, scanning electron microscopy, and flow cytometry. The results we obtained confirmed the initial cytotoxicity of the zinc oxide-eugenol-based sealer and showed that the calcium oxide-based compound had the same relative cytocompatibility as calcium hydroxide.
Guigand
M
M
Department of Endodontics, Faculty of Dentistry, University of Rennes, France.
Pellen-Mussi
P
P
Le Goff
A
A
Vulcain
J M
JM
Bonnaure-Mallet
M
M
D016475:3T3 Cells / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004341:Drug Evaluation; D005434:Flow Cytometry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
D017610:Calcium Compounds; D010087:Oxides; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C016538:lime; D002126:Calcium Hydroxide