Comparison of detection sensitivity of immuno- and genotoxicological effects of subacute cypermethrin and permethrin exposure in rats.
Immuno- and genotoxicological effects of a 28-day oral treatment by equitoxic (1/10, 1/25, 1/50 LD50) doses of cypermethrin (55.4, 22.2, and 11.1 mg/kg) and permethrin (125.7, 50.3, and 12.6 mg/kg) were compared on male Wistar rats. Humoral and cell-mediated immunity were investigated by PFC assay and delayed type hypersensitivity (DTH) reaction (footpad swelling assay), and the genotoxic effects were studied by structural and numerical chromosome aberrations in bone marrow cells. The experimental system also involved certain general toxicological (body weight gain, organ weights) and haematological [white blood cell (WBC), red blood cell (RBC), haematocrit (Ht) and cell content of the femoral bone marrow] investigations. Among the immune function assays, only DTH reaction decreased at the two higher cypermethrin (CY) doses. These doses also increased the number of numerical chromosome aberrations of the bone marrow cells but did not change the number of structural aberrations. All CY doses decreased the mean cell volume (MCV) of RBCs and the Ht value. The two higher doses also reduced the WBC count in the peripheral blood. Permethrin (PE), in the applied dose range, had no effect on the examined immune function parameters, but all three doses increased the number of numerical chromosome aberrations. A dose-dependent increase in the liver weight, decreased MCV value, and elevated cell content of the femoral bone marrow were also observed. Under these experimental conditions, examination of chromosome aberrations proved to be less sensitive in detection of exposure by cypermethrin than applied immune function assays did. Permethrin, on the contrary, increased the number of numeric aberrations at all dose levels but had no effect on the immune function parameters examined.
Institóris
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Department of Public Health and WHO Collaborative Centre for Chemical Safety, Albert Szent-Györgyi Medical University, Szeged, Dóm tér, Hungary. ist@puhe.szote.u-szeged.hu
Undeger
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U
Siroki
O
O
Nehéz
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M
Dési
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I
D000782:Aneuploidy; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002452:Cell Count / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004911:Erythrocyte Volume / Q000187:drug effects; D006400:Hematocrit; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000276:immunology; D007109:Immunity / Q000187:drug effects* / Q000276:immunology; D007306:Insecticides / Q000633:toxicity*; D007958:Leukocyte Count / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000254:growth & development; D008297:Male; D009929:Organ Size / Q000187:drug effects; D026023:Permethrin; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests
D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin; D026023:Permethrin