title: Crystallization and preliminary x-ray diffraction studies of guanidinoacetate methyltransferase from rat liver.
abstract: Guanidinoacetate methyltransferase is the enzyme which catalyzes the last step of creatine biosynthesis. The enzyme is found ubiquitously and in abundance in the livers of all vertebrates. Recombinant rat-liver guanidinoacetate methyltransferase has been crystallized with guanidinoacetate and S-adenosylhomocysteine. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 54.8, b = 162.5, c = 56.1 A, beta = 96.8 (1) degrees at 93 K, and typically diffract beyond 2.8 A.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045-0045, USA.
authors.forename: J
authors.initials: J
authors.lastname: Komoto
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: Y
authors.initials: Y
authors.lastname: Takata
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Gomi
authors.forename: M
authors.initials: M
authors.lastname: Fujioka
authors.forename: F
authors.initials: F
authors.lastname: Takusagawa
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D005998:Glycine / Q000031:analogs & derivatives; D050937:Guanidinoacetate N-Methyltransferase; D008099:Liver / Q000201:enzymology; D008780:Methyltransferases / Q000737:chemistry*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D012435:S-Adenosylhomocysteine / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D012435:S-Adenosylhomocysteine; D008780:Methyltransferases; C494506:GAMT protein, human; C494508:Gamt protein, rat; D050937:Guanidinoacetate N-Methyltransferase; C004946:glycocyamine; D005998:Glycine