Unexpected ratio of allozyme expression in diploid and triploid individuals of the clonal hybrid fish Phoxinus eos-neogaeus.
Phoxinus eos-neogaeus, a North American freshwater fish, was formed by hybridization between P. neogaeus and P. eos. Individuals of P. eos-neogaeus express one allozyme of P. eos and one allozyme of P. neogaeus for enzymes for which the parental allozymes are distinctive. We performed densitometry on phosphoglucomutase (PGM) and one glucose-6-phosphate isomerase locus (GPI-A) separated by cellulose acetate electrophoresis to determine if the parental species' allozymes are expressed in proportion to the number of genomes present in diploid and triploid individuals, and if these enzymes are regulated separately in different tissues. In diploids, activity of the P. eos allozyme was greater than the P. neogaeus allozyme in eye, liver, and muscle but not in heart (one sample t-test, P = 0.05) for PGM. The activity of the P. eos GPI-A allozyme was significantly greater than the P. neogaeus allozyme in heart, eye and muscle but not in liver (one sample t-test, P = 0.05). The expected ratio of eos:neogaeus expression in triploid P. eos-neogaeus x eos individuals is 2:1. For PGM, the observed ratio of eos:neogaeus expression was not significantly different from 2:1 in all four tissues. The P. eos allozyme for GPI was expressed less than expected in all four tissues (one-sample t-test, P = 0.05). Thus, greater than expected expression of the P. eos allozyme was not observed in triploid individuals as it was in the diploids. These data show that PGM and GPI are regulated separately, and that regulation differs by tissue, and in fish of distinct ploidy levels. J. Exp. Zool. 284:663-674, 1999.
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Department of Biology, Ursinus College, Collegeville, Pennsylvania 19426, USA.
Fecteau
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Haws
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Reed
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Hopkins
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Coleman
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Goddard
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D000818:Animals; D002469:Cell Separation; D019976:Cloning, Organism; D003530:Cyprinidae / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004171:Diploidy; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005434:Flow Cytometry; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism*; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D010733:Phosphoglucomutase / Q000235:genetics / Q000378:metabolism*; D011123:Polyploidy
D007527:Isoenzymes; D004247:DNA; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase