Synthesis of an artificial glycoconjugate polymer carrying Pk-antigenic trisaccharide and its potent neutralization activity against Shiga-like toxin.
Fluorescence-labeled glycoconjugate polymers carrying carbohydrate segments of a globotriaosyl ceramide (Gb3) were synthesized and subjected to biological assays using Escherichia coli O-157 strains and Shiga-like toxins (Stx-I and Stx-II). For the fluorescence labeling, a new polymerizable fluorescent monomer with a TBMB carbonyl chromophore (Ex. 325 nm, Em. 410 nm) was designed. A glycosyl monomer of the trisaccharide segment of Gb3 was prepared from p-nitrophenyl beta-lactoside and copolymerized with acrylamide and the fluorescent monomer to prepare a fluorescence-labeled glycoconjugate copolymer carrying [alpha-D-galactopyranosyl-(1-->4)-beta-D-galactopyranosyl]-(1-->4)-beta- D-glucopyranoside. The polymer showed potent neutralization activity against Stx-I and also binding activity onto E. coli O-157 strains.
Dohi
H
H
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Japan.
Nishida
Y
Y
Mizuno
M
M
Shinkai
M
M
Kobayashi
T
T
Takeda
T
T
Uzawa
H
H
Kobayashi
K
K
D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D019453:Escherichia coli O157 / Q000276:immunology; D005453:Fluorescence; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology; D011108:Polymers; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D013057:Spectrum Analysis
D000941:Antigens; D001427:Bacterial Toxins; D006001:Glycoconjugates; D009844:Oligosaccharides; D011108:Polymers; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2