Cloning of a cDNA for xDOR2, a novel TR2-related nuclear orphan receptor, expressed during neurulation in Xenopus laevis embryos.
We isolated from neurulating Xenopus laevis (X. laevis) embryos a cDNA encoding a novel nuclear orphan receptor, Developmental Orphan Receptor 2 (xDOR2), closely related to Ambystoma mexicanum DORI (aDOR1) and to Testicular Receptor-2 (TR2) orphan receptor family members. The xDOR2 cDNA sequence which is truncated both at its 5' and 3' ends predicts a protein sequence of 542 amino acids. While the DNA-binding domain of xDOR2 shares 91%, and 92%, identity with those of aDOR1 and the TR2s, respectively, considerable divergence is observed at both extremities of the peptides. At the N-terminus, xDOR2 is 66% identical to aDOR1 and 64% to the TR2s. At the C-terminus, xDOR2 which is longer by 126 amino acids compared to aDOR1, shares 62%, identity with aDOR1 and appears to contain a complete ligand-binding domain. The DOR receptors appear to form a subtype distinct from the TR2s, and may play a role in the development and differentiation of neural tissues.
Huard
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Centre de recherche en cancérologie de l'Université Laval, Centre hospitalier universitaire de Québec, Canada.
Séguin
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D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology; D057107:Nuclear Receptor Subfamily 2, Group C, Member 1; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics*
D018076:DNA, Complementary; C400782:NR2C1 protein, Xenopus; C539822:NR2C1 protein, human; D057107:Nuclear Receptor Subfamily 2, Group C, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D011988:Receptors, Thyroid Hormone; D029867:Xenopus Proteins