Differential expression of caprine beta-defensins in digestive and respiratory tissues.
We identified two novel beta-defensin precursors, preproGBD-1 and preproGBD-2, in the tissues of a goat. Although the precursors were identical in 96.8% of their bases and 88.2% (60 of 68) of their amino acids, preproGBD-1 was expressed principally in the tongue and respiratory tract, whereas preproGBD-2 expression predominated throughout the intestine. These findings exemplify the phenomenon of tissue-specific expression in a family of host defense peptides that arose before the avian and mammalian lineages diverged.
Zhao
C
C
Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA.
Nguyen
T
T
Liu
L
L
Shamova
O
O
Brogden
K
K
Lehrer
R I
RI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D023082:Defensins; D004064:Digestive System / Q000276:immunology*; D006041:Goats; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D012137:Respiratory System / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D023082:Defensins; D011506:Proteins