Empirical midpoint dissociation temperature (Td) determination for oligonucleotide probes using a PCR thermal cycler.
Gulledge
J
J
Harvard University, Cambridge, MA, USA.
Cavanaugh
C M
CM
D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013696:Temperature; D013816:Thermodynamics
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S