Fucose in alpha(1-6)-linkage regulates proliferation and histogenesis in reaggregated retinal spheroids of the chick embryo.
We have used the lectin from Aleuria aurantia (AAL) which is highly specific for alpha(1-6)-linked fucose, to examine its effect on chicken retinogenesis in a reaggregation culture system. When dispersed cells of the embryonic chick retina are reaggregated to form histotypic retinospheroids, AAL elicits strong inhibition of spheroid growth. The action of AAL is specific, since its effect is dose-dependent, saturable, and inhibited by an excess of fucose. Fucosidase treatment entirely abolishes reaggregation. In contrast, Anguilla anguilla agglutinin (AAA) binding to fucose in alpha(1-2)-linkage does not show any effects. Incubation with CAB4-a specific monoclonal antibody for fucose in alpha(1-6)-linkage-reduces spheroid size and shape. AAL does not much affect primary aggregation, but rather subsequent processes of cell proliferation and histogenesis. In particular, AAL inhibits uptake of bromo-desoxyuridine (BrdU), most efficiently so during days in vitro 2 (div2) and div3. As a consequence, the histological differentiation is entirely disturbed, as evidenced by vimentin immunostaining; particularly, rosettes are not forming and the radial glia scaffold is disorganized. We conclude that glycoproteins exhibiting fucose in alpha(1-6)-linkage may play major roles in early processes of retinal tissue formation.
Stelck
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S
Darmstadt University of Technology, Faculty of Biology, Department of Developmental Biology & Neurogenetics, Schnittspahnstrasse 3, D-64287 Darmstadt, Germany.
Robitzki
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A
Willbold
E
E
Layer
P G
PG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002236:Carbohydrate Conformation; D002449:Cell Aggregation; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology; D002642:Chick Embryo; D005643:Fucose / Q000276:immunology / Q000502:physiology*; D037102:Lectins / Q000494:pharmacology*; D009940:Organoids / Q000187:drug effects / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D014746:Vimentin / Q000032:analysis; D005644:alpha-L-Fucosidase / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D037102:Lectins; D011134:Polysaccharides; D014746:Vimentin; C064549:lectin, Aleuria aurantia; D005643:Fucose; D005644:alpha-L-Fucosidase; D001973:Bromodeoxyuridine