title: A structural comparison of lipopolysaccharides from two strains of Helicobacter pylori, of which one strain (442) does and the other strain (471) does not stimulate pepsinogen secretion.
abstract: Lipopolysaccharides (LPSs) from strains of Helicobacter pylori (442 and 471), which differed in stimulation of pepsinogen secretion, were isolated as water-soluble material of high-M(r), and as water-insoluble gels of low-M(r). Chemical and spectroscopic analyses of soluble LPS and oligosaccharides liberated from the gels led to proposed structures with Lewis (Le) antigen termini connected to N -acetyllacto-saminoglycans of alternating 3-linked beta-D-Gal and 4-linked beta-D-GlcNAc residues with various laterally attached glycosyl substituents. The LPS of H.pylori 442 was similar to previously examined strains (NCTC 11637 and P466) in having partially glycosylated chains with alpha-L-Fuc units attached to O-3 of the majority of GlcNAc residues in Le(x)units, and in chain termination with Le(x)or Le(y)determinants. In contrast, terminal Le(y)units occurred in LPS of H.pylori 471 and glycosaminoglycan chains carried a smaller proportion of alpha-L-Fuc units, but at O-6 of a majority of nonfucosylated GlcNAc residues, there was a novel type of branching with alpha-D-Gal substituents. Evidence for the branched regions was obtained from(1)H-NMR spectra and from characterization of oligosaccharides formed by the action of endo-beta-galactosidase. Examination of oligosaccharides liberated from water-insoluble LPS gels of H.pylori 442 and 471 provided evidence for similar core OS structures to those from other H.pylori strains but interesting differences were observed.
authors.affiliation: Department of Chemistry, York University, North York, ON M3J 1P3, Canada.
authors.forename: G O
authors.initials: GO
authors.lastname: Aspinall
authors.forename: A S
authors.initials: AS
authors.lastname: Mainkar
authors.forename: A P
authors.initials: AP
authors.lastname: Moran
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004381:Duodenal Ulcer / Q000382:microbiology; D006031:Glycosylation; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D009844:Oligosaccharides / Q000032:analysis; D020116:Pepsinogen A / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects; D012995:Solubility; D013045:Species Specificity; D013268:Stimulation, Chemical
chemical_list: D000942:Antigens, Bacterial; D007983:Lewis Blood Group Antigens; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D020116:Pepsinogen A