title: [Double-strand-primered polymerase chain reaction].
abstract: OBJECTIVE
  To develop a simple and reliable method for DNA amplification.
  
  
  METHODS
  Double-strand-primers of fragile X mental retardation 1 gene (FMR1) were generated and used to amplify DNAs that were roughly extracted from human peripheral blood.
  
  
  RESULTS
  All products from 15 DNA samples by double-strand-primered polymerase chain reaction (DSP-PCR) were more specific than those amplified by routine PCR.
  
  
  CONCLUSIONS
  The double-strand-primers reported here may increase the specificity of PCR amplification.
authors.affiliation: Department of Medical Genetics, The Affiliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P.R.China. zszg@wcums.edu.cn
authors.forename: Q
authors.initials: Q
authors.lastname: Xia
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Ding
authors.forename: H
authors.initials: H
authors.lastname: Wu
mesh_terms: D006801:Humans; D016133:Polymerase Chain Reaction