Identification of synthetic by-products in combinatorial libraries using high performance liquid chromatography-electrospray ionization mass spectrometry.
High performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI) and high performance liquid chromatography coupled to mass spectrometry (LC-MS) were used to analyze randomly chosen samples from parallel syntheses carried out on derivatized polypropylene crowns compatible with a Multipin solid support system. Side-reactions and by-products were clearly identified, and the yields of the expected molecules were unexpectedly low for most samples. LC-MS was superior to HPLC with absorbance detection or electrospray mass spectrometry alone for determining the identity and purity of each desired combinatorial compounds.
Aubagnac
J L
JL
UMR 5810 - Laboratoire des Aminoacides, Peptides et Protéines - Universités Montpellier I et II, Place E. Bataillon, 34095 Montpellier Cédex 05, France. aubagnac@univ-montp2.fr
Amblard
M
M
Enjalbal
C
C
Subra
G
G
Martinez
J
J
Durand
P
P
Renaut
P
P
D000178:Acrylamides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D004988:Ethers, Cyclic / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D019151:Peptide Library; D011126:Polypropylenes / Q000737:chemistry
D000178:Acrylamides; D000596:Amino Acids; D004988:Ethers, Cyclic; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D019151:Peptide Library; D011126:Polypropylenes