In vivo production of nitric oxide after administration of cyclohexanone oxime.
Cyclohexanone oxime (CHOX), an intermediate used in the synthesis of polycaprolactam (Nylon-6), has been reported to be hematotoxic in Fischer rats. The in vivo metabolism of CHOX was found to release nitric oxide, which was detected in venous blood by electron paramagnetic resonance spectroscopy as the nitrosylhemoglobin complex. In vitro incubation of CHOX with venous blood resulted in the formation of the characteristic nitrosylhemoglobin complex, suggesting that the blood was a possible site for metabolism. Excessive nitric oxide production may, in part, contribute to the observed toxicity of CHOX.
Glover
R E
RE
Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Science, National Institutes of Health, P.O. Box 12233, Research Triangle Park, North Carolina 27709, USA. glover1@niehs.nih.gov
Corbett
J T
JT
Burka
L T
LT
Mason
R P
RP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003512:Cyclohexanones / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D003512:Cyclohexanones; D003580:Cytochromes; D006454:Hemoglobins; C044686:cyclohexanone oxime; D009569:Nitric Oxide; C100003:deoxyhemoglobin; D008706:Methemoglobin; C013464:cytochrome P420; D003577:Cytochrome P-450 Enzyme System