Effect of linear polarized near-infrared ray irradiation on the chemiluminescence of human neutrophils and serum opsonic activity.
The purpose of this study was to investigate the in vitro effects of linear polarized near-infrared ray irradiation on neutrophil chemiluminescence (CL) and serum opsonic activity. We used luminol- and lucigenin-dependent CL to detect the affected reactive oxygen species production process of human neutrophils and measured serum opsonic activity based on luminol-dependent CL. The linear polarized near-infrared ray irradiation suppressed a maximum light emission (peak height) of luminol- and lucigenin-dependent CL in a dose-dependent manner. The findings suggested that the linear polarized near-infrared ray irradiation suppressed the superoxide anion and hypochlorite production of human neutrophils. The serum opsonic activity was decreased by linear polarized near-infrared ray irradiation, and this suppressive effect might be caused by inhibiting the activation of the classical and alternative complement pathway. Therefore, it is suggested that near-infrared ray irradiation may have an inhibitory effect against chronic pain via reduction of reactive oxygen species production and opsonic activity.
Shiraishi
M
M
Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki City, 036-8562 Japan.
Suzuki
K
K
Nakaji
S
S
Sugawara
K
K
Sugita
N
N
Suzuki
K J
KJ
Ohta
S
S
D000166:Acridines; D006801:Humans; D006997:Hypochlorous Acid / Q000097:blood; D007259:Infrared Rays; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000502:physiology / Q000528:radiation effects*; D009895:Opsonin Proteins / Q000097:blood / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000502:physiology; D013481:Superoxides / Q000097:blood
D000166:Acridines; D009895:Opsonin Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D006997:Hypochlorous Acid