GABAA receptor modulation of temperature sensitive neurons in the diagonal band of Broca in vitro.
Several regions of the brain, including the horizontal limb of the diagonal band of Broca (HDB), contain neurons that are responsive to changes in local temperature. These neurons are hypothesized to participate in thermoregulation and sleep-wake control. The HDB contains a large number of gamma-aminobutyric acid (GABA) terminals, and it has many neurons that utilize GABA as a neurotransmitter. Therefore, in this study we characterized the in vitro effects of the GABAA receptor agonist muscimol (0.5, 0.25, 0.1 and 0.0625 microM doses) and the GABAA receptor antagonist bicuculline (3.0 and 1.0 microM doses) on the firing rate and thermosensitivity of HDB neurons. Of the 51 neurons recorded in a submerged slice chamber, 53% were warm sensitive, 45% were temperature insensitive and 2% were cold sensitive. All neurons exposed to bath applied muscimol exhibited reductions in both firing rate and thermosensitivity. Muscimol induced reductions were maintained for at least 20 min after washout. Neurons exposed to bicuculline had no change in firing rate or thermosensitivity. However, after bicuculline washout there were reductions in both firing rate and thermosensitivity. These findings support the hypothesis that GABAA receptor induced inhibition of HDB thermosensitive neurons can modulate both thermoregulation and sleep-wake control.
Hays
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TC
Interdepartmental Program in Neuroscience, University of California-Los Angeles, Los Angeles, CA, USA.
Szymusiak
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R
McGinty
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D
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001833:Body Temperature Regulation / Q000502:physiology*; D020667:Diagonal Band of Broca / Q000033:anatomy & histology / Q000166:cytology* / Q000502:physiology; D004594:Electrophysiology; D018755:GABA Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009118:Muscimol / Q000494:pharmacology; D009474:Neurons / Q000737:chemistry* / Q000502:physiology*; D011301:Preoptic Area / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000502:physiology*; D012890:Sleep / Q000502:physiology; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D018755:GABA Agonists; D018756:GABA Antagonists; D011963:Receptors, GABA-A; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D001640:Bicuculline