Isolation and analysis of a novel acidic polysaccharide from the case of squid pen.
A new non-sulphated acidic polysaccharide with an average molecular mass of 55 kDa was isolated from squid pen case after papain digestion and beta-elimination. This polysaccharide contains mainly L-iduronic acid, D-glucuronic acid, D-galactosamine, D-glucosamine and significant amounts of neutral sugars as glucose, galactose and fucose. The polysaccharide was not degraded to the relative disaccharides by chondroitinases ABC, AC and B, hyaluronidase and keratanase or by treatment with heparinases, suggesting a structure different from those of known glycosaminoglycans. The polysaccharide cannot form self aggregates.
Theocharis
A D
AD
Department of Chemistry, University of Patras, Greece.
Karamanos
N K
NK
Tsegenidis
T
T
D000818:Animals; D002240:Carbohydrate Sequence; D002356:Cartilage / Q000737:chemistry*; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D006025:Glycosaminoglycans / Q000032:analysis; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D014574:Uronic Acids / Q000032:analysis
D006025:Glycosaminoglycans; D011134:Polysaccharides; D014574:Uronic Acids; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase