Regulatory T cells in experimental allergic encephalomyelitis. II. T cells functionally antagonistic to encephalitogenic MBP-specific T cells show persistent expression of fasL.
Naive LEW rats and animals that have recovered from active or adoptive EAE contain a subset of T cells that inhibit EAE in secondary recipients and are lytic for MBP-reactive T cell lines in culture. Here we explore various features of these regulatory T cell populations (RTC) with respect to (1) their frequency in animals following immunization with syngeneic MBP-reactive cell lines, (2) their ability to inhibit proliferative responses by MBP-reactive cell lines in culture, (3) their ability to lyse MBP-specific target cells, and especially (4) their prolonged expression of high levels of FasL following activation in culture correlating with their lytic effects on A20, a FasL-sensitive mouse lymphoma cell line. Inhibition studies indicate that mAbs specific for MHC class I and MHC class II molecules inhibit lysis of syngeneic MBP-reactive target T cells, soluble Fas protein shows some inhibition, but none of these agents inhibits the lytic effects of activated RTC on the A20 cell line.
Sun
D
D
Department of Neurology and the Center for Neuroimmunology of the University of Alabama at Birmingham 35294, USA. dsun@uab.edu
Whitaker
J N
JN
Wilson
D B
DB
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D053222:Fas Ligand Protein; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000276:immunology
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; C505662:Faslg protein, rat; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D004676:Myelin Basic Protein; D019014:fas Receptor