title: Nitric oxide and renal nerve-mediated proximal tubular reabsorption in normotensive and hypertensive rats.
abstract: In Inactin-anesthetized Wistar rats with an intact renal innervation, intratubular nitro-L-arginine methyl ester (L-NAME, 10(-4) M) increased proximal fluid uptake (J(va), at 2.47 +/- 0.61 x 10(-4) mm(3). mm(-2). s(-1)) by 17% (P < 0.05), whereas coadministration with sodium nitroprusside (SNP, 10(-4) M) decreased J(va) by 18% (P < 0.01). Similar manipulation of NO generation was without effect in groups of Wistar rats subjected to acute renal denervation. Intratubular aminoguanidine (10(-4) M), a selective inducible nitric oxide synthase (NOS) blocker, had no effect on J(va) in intact kidneys of Wistar rats, but the neuronal NOS (nNOS) blocker, 7-nitroindazole (10(-4) M and 10(-6) M) increased J(va) by 19-23% (both P < 0.001). In stroke-prone spontaneously hypertensive rats (SHRSP), J(va) values in the innervated kidneys were lower (P < 0.05) than in the corresponding Wistar groups and were unchanged by intratubular L-NAME or L-NAME plus SNP. The tonic attenuation of proximal epithelial transport by NO was dependent on the renal sympathetic nerves and appeared to be generated by the nNOS isoform of the enzyme. This role of NO was not evident in the SHRSP.
authors.affiliation: Department of Physiology, University of Birmingham, Birmingham B15 2TT, United Kingdom.
authors.forename: X C
authors.initials: XC
authors.lastname: Wu
authors.forename: P J
authors.initials: PJ
authors.lastname: Harris
authors.forename: E J
authors.initials: EJ
authors.lastname: Johns
mesh_terms: D000042:Absorption / Q000502:physiology; D000818:Animals; D020022:Genetic Predisposition to Disease; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000294:innervation*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D009420:Nervous System / Q000503:physiopathology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000502:physiology; D052248:Nitric Oxide Synthase Type I; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics; D017208:Rats, Wistar; D012016:Reference Values; D020521:Stroke / Q000235:genetics
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat