Detection of lysergic acid diethylamide (LSD) in urine by gas chromatography-ion trap tandem mass spectrometry.
A confirmatory method for the detection and quantitation of lysergic acid diethylamide (LSD) is presented. The method employs gas chromatography-tandem mass spectrometry (GC-MS-MS) using an internal ionization ion trap detector for sensitive MS-MS-in-time measurements of LSD extracted from urine. Following a single-step solid-phase extraction of 5 mL of urine, underivatized LSD can be measured with limits of quantitation and detection of 80 and 20 pg/mL, respectively. Temperature-programmed on-column injections of urine extracts were linear over the concentration range 20-2000 pg/mL (r2 = 0.999). Intraday and interday coefficients of variation were < 6% and < 13%, respectively. This procedure has been applied to quality-control specimens and LSD-positive samples in this laboratory. Comparisons with alternate GC-MS methods and extraction procedures are discussed.
Sklerov
J H
JH
Office of the Armed Forces Medical Examiner, Division of Forensic Toxicology, Armed Forces Institute of Pathology, Rockville, Maryland 20850-3125, USA.
Kalasinsky
K S
KS
Ehorn
C A
CA
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008238:Lysergic Acid Diethylamide / Q000302:isolation & purification / Q000652:urine*; D015203:Reproducibility of Results; D013696:Temperature
D008238:Lysergic Acid Diethylamide