Sequencing of 42kb of the APO E-C2 gene cluster reveals a new gene: PEREC1.
Through the sequencing of a 42kb cosmid clone we describe a new gene, designated PEREC1, located approximately 1.5kb centromeric of the human apolipoprotein (APO) E-C2 cluster. The combination of dotplot analysis, predicted coding potential and interrogation of the Expressed Sequence Tag (EST) database determined the genomic organisation of PEREC1. Sequence alignment with multiple overlapping ESTs confirmed the predicted splice sites. The predicted cDNA and amino acid sequences of PEREC1 have extensive similarity to the Caenorhabditis elegans protein, C18E9.6. Conserved structural and functional motifs have been defined by combining nucleotide and amino acid analyses to identify third base degeneracy and therefore selection at the protein level. The Poliovirus Receptor Related Protein2 gene (PRR2), previously mapped to chromosome 19q13.2 by Fluorescent In-Situ Hybridisation, has also been located approximately 17kb centromeric of APO E.
Freitas
E M
EM
Centre for Molecular Immunology and Instrumentation, University of Westem Australia, Nedlands.
Zhang
W J
WJ
Lalonde
J P
JP
Tay
G K
GK
Gaudieri
S
S
Ashworth
L K
LK
Van Bockxmeer
F M
FM
Dawkins
R L
RL
D000544:Alzheimer Disease / Q000235:genetics; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics; D015815:Cell Adhesion Molecules; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003327:Coronary Disease / Q000235:genetics; D003360:Cosmids / Q000235:genetics; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D026901:Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D000075983:Nectins; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D001057:Apolipoproteins E; D015815:Cell Adhesion Molecules; D015801:Helminth Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D000075983:Nectins; D011506:Proteins; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human; C400783:TOMM40 protein, human