Unique expression patterns of myosin heavy chain genes in the ductus arteriosus and uterus of rabbits.
In smooth muscle tissue, two smooth muscle myosin heavy chain (MHC) isoforms (SM1, SM2) and two non-muscle MHC isoforms (NMA, NMB) have been identified. The purpose of our study was to clarify whether smooth muscle MHC mRNA expression reflects the physiological and functional state of the muscle. We studied the expression pattern of MHC mRNAs, using the S1-nuclease mapping procedure, in functionally and morphologically changeable organs; the ductus arteriosus (DA) during development (25 and 29 days of gestation, and from 3-day-old neonates) and uteri from virgin, day-10 pregnant (P10) and day-29 pregnant (P29) rabbits. The results demonstrated that SM2 expression was greater in the fetal DA than in the fetal aortic and pulmonary arteries, but that it decreased significantly following closure of DA. In the gravid uterus, SM1 expression was significantly (P<0.05) strong compared to other MHC mRNAs from virgin to P10 rabbits. During pregnancy, NMB expression showed a tendency to increase until P10, and after P10, SM2 expression increased dramatically and NMB expression decreased to give almost a mirror image of the SM2 expression. Smooth muscle type (SM1, SM2) was significantly (P<0.05) strong compared to non-muscle type expression (NMA, NMB) at P29. These data suggest that smooth muscle MHC mRNA, especially SM2 expression reflects the physiological and functional state of the smooth muscle.
Sakurai
H
H
Department of Pediatric Cardiology, The Heart Institute of Japan, Tokyo Women's Medical University.
Imamura
S
S
Furutani
Y
Y
Takao
A
A
Momma
K
K
Matsuoka
R
R
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015152:Blotting, Northern / Q000662:veterinary; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004373:Ductus Arteriosus / Q000502:physiology*; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000235:genetics*; D009710:Nucleotide Mapping; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D011817:Rabbits; D017422:Sequence Analysis, DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry; D014599:Uterus / Q000502:physiology*
D017931:DNA Primers; D015342:DNA Probes; D012313:RNA; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases; D018995:Myosin Heavy Chains