[The biosynthesis of 2H-labelled inosine by Bacillus subtilis bacteria in a heavy-hydrogen medium].
We studied biosynthesis of the purine ribonucleoside inosine by the strain of Bacillus subtilis, which was adapted to deuterium through plating to individual colonies on 2% agar with 2H2O and subsequent selection. For growing the strain, a special heavy hydrogen medium with a high level of deuteration (89-90 at. % 2H) based on 2% hydrolyzate of the biomass of the methylotrophic Brevibacterium methylicum as a source of 2H-labeled growth substrates was obtained on 98 vol % 2H2O and 2 vol % [U-2H] methanol. We provide experimental data on the strain growth and accumulation of inosine in the culture medium. A study of the level of inosine deuteration using mass-spectroscopy of fast atoms has shown a polymorphism of deuterium incorporation in the molecule (isotope composition of inosine: 4 at. 2H, 20%; 5 at. 2H, 38%; 6 at. 2H, 28%; 7 at. 2H, 14%), deuterium being incorporated in the ribose and hypoxanthine fragments of the molecules.
Mosin
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Lomonosov State Academy of Fine Chemical Technology, Moscow, Russia.
Skladnev
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Shvets
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D000596:Amino Acids / Q000032:analysis; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D001951:Brevibacterium / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000706:statistics & numerical data; D003470:Culture Media; D003903:Deuterium; D006863:Hydrogen-Ion Concentration; D007288:Inosine / Q000032:analysis / Q000096:biosynthesis*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000706:statistics & numerical data; D013696:Temperature; D013997:Time Factors
D000596:Amino Acids; D002241:Carbohydrates; D003470:Culture Media; D007288:Inosine; D003903:Deuterium