title: All-trans-retinoic-acid- and growth-factor- mediated induction of alkaline phosphatase activity in freshly isolated chronic myeloid leukemia cells.
abstract: Reduced or absent neutrophil alkaline phosphatase (NAP) activity is a common feature of neutrophilic granulocytes from patients with chronic myeloid leukemia (CML). In this study we examined whether NAP activity could be restored in vitro by stimulating CML cells with different promoters such as all-trans-retinoic acid (ATRA), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). The results obtained indicated that ATRA and G-CSF, either alone or in combination, were effective in inducing NAP activity in CML cells, whereas GM-CSF was not. Further, NAP restoration in ATRA- and G-CSF-treated cultures was accompanied by increased morphologic differentiation of the CML clone. It might be concluded that the CML clone could be driven in vitro by ATRA and G-CSF both to achieve granulocytic maturation and to correct functional NAP-related defects.
authors.affiliation: Chair of Hematology, University of Catania, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Stagno
authors.forename: P
authors.initials: P
authors.lastname: Guglielmo
authors.forename: U
authors.initials: U
authors.lastname: Consoli
authors.forename: P
authors.initials: P
authors.lastname: Fiumara
authors.forename: G S
authors.initials: GS
authors.lastname: Longo
authors.forename: R
authors.initials: R
authors.lastname: Giustolisi
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000187:drug effects*; D002469:Cell Separation; D004790:Enzyme Induction; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D006133:Growth Substances; D014212:Tretinoin; D000469:Alkaline Phosphatase