[Qualitative change in the elastin from the calcified portion of human artery].
To examine the qualitative changes of elastin and the aorta related to calcification of human arteries, biochemical properties were measured, including calcium (Ca), phosphorus (P) and magnesium (Mg) contents in the aorta or in the elastin fraction in calcification, cholesterol content in atherosclerosis, desmosine content of cross-link, free thiol contents (free SH/total SH) and hydrophobic properties in the elastin fraction from the calcified portion, adjacent sites and another normal artery. The results from different sites of the calcified abdominal artery are as follows: The contents of Ca, P and Mg in aorta and the elastin fraction from the calcification site were higher than those at other sites. Moreover, Ca in the aorta and elastin fraction correlated positively with P and Mg. The content of cholesterol in the calcification site was the same as at other sites and did not correlate with Ca, P or Mg. The content of desmosine in the calcification site was significantly lower than that in different sites. In addition, its content was negatively associated with Ca and P in the elastin fraction and with the aortic Mg. The content of free thiol in the calcification site was similar to the other sites and correlated negatively with Ca and P in the aorta. The hydrophobicity in the calcification was similar to that at other sites, and was negatively associated with Ca and Mg in the elastin fraction.
Hayashi
M
M
Chigasaki City Hospital, Kanagawa, Japan.
Osakabe
T
T
Ushio
F
F
Seyama
Y
Y
D000368:Aged; D000369:Aged, 80 and over; D001011:Aorta / Q000737:chemistry*; D001012:Aorta, Abdominal / Q000737:chemistry; D001018:Aortic Diseases / Q000378:metabolism*; D002114:Calcinosis / Q000378:metabolism*; D002118:Calcium / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D003895:Desmosine / Q000032:analysis; D004549:Elastin / Q000032:analysis*; D006801:Humans; D008274:Magnesium / Q000032:analysis; D008875:Middle Aged; D010758:Phosphorus / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000032:analysis
D013438:Sulfhydryl Compounds; D003895:Desmosine; D010758:Phosphorus; D004549:Elastin; D002784:Cholesterol; D008274:Magnesium; D002118:Calcium