Spliced leader-associated RNA from Crithidia fasciculata contains a structure resembling stem/loop II of U1 snRNA.
In contrast to earlier proposals, recent evidence suggests that trans-spliceosomes in trypanosomatid protozoa may contain a homolog of U1 small nuclear (sn) RNA (Schnare, M.N. and Gray, M.W. (1999) J. Biol. Chem. 274, 23,691-23,694). However, the candidate trypanosomatid U1 snRNA is unconventional because it lacks the highly conserved stem/loop II present in all other U1 snRNAs. Trypanosomatids also possess a unique spliced leader-associated (SLA) RNA of unknown function. We present the complete sequence of the SLA RNA from Crithidia fasciculata and propose that it may contribute a U1 snRNA-like stem/loop II to the trans-spliceosome.
Schnare
M N
MN
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, N.S., Canada.
Gray
M W
MW
D000818:Animals; D001483:Base Sequence; D016831:Crithidia fasciculata / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016053:RNA, Protozoan / Q000737:chemistry* / Q000235:genetics; D012342:RNA, Small Nuclear / Q000737:chemistry; D020038:RNA, Spliced Leader / Q000737:chemistry* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D016053:RNA, Protozoan; D012342:RNA, Small Nuclear; D020038:RNA, Spliced Leader