Production of 2,3-butanediol by newly isolated Enterobacter cloacae.
Enterobacter cloacae NRRL B-23289 was isolated from local decaying wood/corn soil samples while screening for microorganisms for conversion of L-arabinose to fuel ethanol. The major product of fermentation by the bacterium was meso-2,3-butanediol (2,3-BD). In a typical fermentation, a BD yield of 0.4 g/g arabinose was obtained with a corresponding productivity of 0.63 g/l per hour at an initial arabinose concentration of 50 g/l. The effects of initial arabinose concentration, temperature, pH, agitation, various monosaccharides, and multiple sugar mixtures on 2,3-BD production were investigated. BD productivity, yield, and byproduct formation were influenced significantly within these parameters. The bacterium utilized sugars from acid plus enzyme saccharified corn fiber and produced BD (0.35 g/g available sugars). It also produced BD from dilute acid pretreated corn fiber by simultaneous saccharification and fermentation (0.34 g/g theoretical sugars).
Saha
B C
BC
National Center for Agricultural Utilization Research, United States Department of Agriculture, Peoria, IL 61604, USA. sahabc@mail.ncaur.usda.gov
Bothast
R J
RJ
D000143:Acids / Q000494:pharmacology; D001089:Arabinose / Q000378:metabolism; D002072:Butylene Glycols / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D016972:Enterobacter cloacae / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D007220:Industrial Waste; D003313:Zea mays
D000143:Acids; D002072:Butylene Glycols; D007220:Industrial Waste; C026978:2,3-butylene glycol; D001089:Arabinose