Mutation analysis of the Smad3 gene in human ovarian cancers.
The Smad3 gene is a member of the Smad family, vertebrate homologues of Drosophila Mad, and its gene product is a cytoplasmic element in the transforming growth factor-beta (TGF-beta) signaling pathway. Mutations in TGF-beta receptors and their cytoplasmic elements of transduction signals commonly accompany various cancers. Using PCR-SSCP analysis we searched for the presence of Smad3 gene mutations in 36 human ovarian cancers, and found that 15 cases (41. 7%) had a polymorphism at codon 103. Because this mutation was not accompanied by amino acid replacement, the present results show that the mutations in the Smad3 gene are unlikely to be involved in human ovarian cancers.
Wang
D
D
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Maebashi, Gunma, Japan.
Kanuma
T
T
Takama
F
F
Mizumuma
H
H
Ibuki
Y
Y
Wake
N
N
Mogi
A
A
Shitara
Y
Y
Hagiwara
K
K
Takenoshita
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila; D005260:Female; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015398:Signal Transduction; D051900:Smad3 Protein; D015534:Trans-Activators / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000502:physiology; D014714:Vertebrates
D017931:DNA Primers; D004268:DNA-Binding Proteins; C497790:SMAD3 protein, human; D051900:Smad3 Protein; D015534:Trans-Activators; D016212:Transforming Growth Factor beta