Pancreatic regeneration after subtotal distal resection in rats. Effects of bombesin and octreotide by the in vivo bromodeoxyuridine uptake technique.
Forty male Wistar rats were randomly allocated to four treatment groups after 90% distal pancreatectomy: group A (control) received saline (0.5 ml subcutaneously); group B received bombesin (BBS; 10 microg/kg intraperitoneally); group C received octreotide (2.5 microg/kg subcutaneously), and group D received BBS and octreotide. All substances were injected three times a day until sacrifice after 28 days. BBS increased pancreas weight (p = 0.003) and DNA synthesis (p < 0.001), as measured by a bromodeoxyuridine nuclear-labeling index (BrdU LI). The simultaneous administration of octreotide significantly decreases the remnant pancreas weight (p = 0.016) as compared to group B rats; however the BrdU LI is not significantly reduced in group D as compared to group B. BBS administration promotes regeneration of the remnant pancreas in terms of hypertrophy and hyperplasia. Although octreotide appears to significantly reduce the pancreatic weight increase induced by BBS, it does not reduce DNA synthesis and cell proliferation.
Bufalari
A
A
Department of Surgical Sciences, University of Perugia, Italy.
Ferri
M
M
Sidoni
A
A
Alberti
P
P
Giustozzi
G
G
Moggi
L
L
D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D005765:Gastrointestinal Agents / Q000494:pharmacology; D008297:Male; D015282:Octreotide / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D010180:Pancreatectomy / Q000379:methods; D011183:Postoperative Complications; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration
D005765:Gastrointestinal Agents; D004247:DNA; D001973:Bromodeoxyuridine; D001839:Bombesin; D015282:Octreotide