title: Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane.
abstract: We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 microsecond and lifetime components as long as 3 microseconds in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2 x 10(-8) cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with microsecond lipid probes.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: I
authors.initials: I
authors.lastname: Gryczynski
authors.forename: J R
authors.initials: JR
authors.lastname: Lakowicz
mesh_terms: D004735:Energy Transfer; D008024:Ligands; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D012211:Rhenium; D013054:Spectrophotometry, Atomic
chemical_list: D008024:Ligands; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D012211:Rhenium; C017251:1,2-oleoylphosphatidylcholine