Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette.
Human erythropoietin (EPO)-producing recombinant BHK cells were cultured in culture medium containing microcarriers, and then microcarriers attached with cells were replenished in the hollow fiber culture cassette. By culture for 14 days, it was possible to produce 450 micrograms of the recombinant EPO, which corresponded to over two-fold of the recombinant EPO production by control hollow fiber culture without microcarriers.
Inoue
Y
Y
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Japan. inoue@ugs.agri.kagoshima-u.ac.jp
Arita
N
N
Teruya
K
K
Katakura
Y
Y
Shirahata
S
S
D000818:Animals; D019149:Bioreactors; D001709:Biotechnology; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D004921:Erythropoietin / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins
D003470:Culture Media; D011994:Recombinant Proteins; D004921:Erythropoietin