DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.
We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold.
Keppler
M D
MD
Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK.
McKeen
C M
CM
Zegrocka
O
O
Strekowski
L
L
Brown
T
T
Fox
K R
KR
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I; D008024:Ligands; D009690:Nucleic Acid Conformation; D011804:Quinolines / Q000737:chemistry*
D008024:Ligands; D011804:Quinolines; C102232:naphthylquinoline triple-helix-binding ligand; D004247:DNA; D003850:Deoxyribonuclease I