A pair of invertedly repeated genes in Chlamydomonas reinhardtii encodes a zygote-specific protein whose expression is UV-sensitive.
Uniparental inheritance of the chloroplast genome has been observed in a wide variety of green plants. In Chlamydomonas this phenomenon, which can be selectively inhibited by UV irradiation of mt(+) gametes, has been shown cytologically to be due to the preferential degradation of mt(-)-derived chloroplast nucleoids in young zygotes. The zygote-specific pair of zys1 genes, zys1A and zys1B, is expressed earliest among five genes isolated from a "10-min" zygote library. We report here that the ZYS1 protein, which is encoded by the invertedly duplicated zys1 gene, accumulates in zygotes and is localized in nuclei. In addition, when mt(+) gametes (but not mt(-) gametes) are UV-irradiated before mating, only very limited accumulation of ZYS1 protein can be detected in the resulting zygotes.
Uchida
H
H
Institute of Biological Sciences, University of Tsukuba, Tsukuba 305-8577, Japan. hide-uchida@mtd.biglobe.ne.jp
Suzuki
L
L
Anai
T
T
Doi
K
K
Takano
H
H
Yamashita
H
H
Oka
T
T
Kawano
S
S
Tomizawa
K I
KI
Kawazu
T
T
Kuroiwa
H
H
Kuroiwa
T
T
D020418:Algal Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D002736:Chloroplasts / Q000235:genetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D014466:Ultraviolet Rays; D015053:Zygote / Q000378:metabolism / Q000528:radiation effects
D020418:Algal Proteins; D010940:Plant Proteins