title: Determination of betaine in Lycium chinense fruits by liquid chromatography-electrospray ionization mass spectrometry.
abstract: A rapid and sensitive high-performance liquid chromatography-electrospray mass spectrometric method has been developed for the determination of betaine in Lycium chinense fruits. Betaine was analyzed on a system consisting of a NH2 stationary phase and a mobile phase of water-acetonitrile (25:75) by isocratic elution for 40 min. Betaine was identified and quantitated by electrospray ionization mass spectrometry with selected ion monitoring of the protonated ion [Betaine+H]+ and clustered ions [nBetaines+H]+. The limit of detection for betaine by this method was ca. 0.2 ng/ml and the relative standard deviations of the assay (intra- and inter-day) were less than 8.1%.
authors.affiliation: College of Pharmacy, Seoul National University, South Korea.
authors.forename: Y G
authors.initials: YG
authors.lastname: Shin
authors.forename: K H
authors.initials: KH
authors.lastname: Cho
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: M K
authors.initials: MK
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Park
mesh_terms: D001622:Betaine / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D001622:Betaine