Purification and characterization of a platelet aggregation inhibitor acidic phospholipase A2 from Indian saw-scaled viper (Echis carinatus) venom.
An acidic phospholipase A2 (EC-I-PLA2) has been purified from the Indian saw-scaled viper (Echis carinatus) venom through a combination of column chromatography and electrophoresis. EC-I-PLA2 has a molecular weight of 16000 by SDS-PAGE. It was focussed between pH 4.2 and 4.8 by isoelectro focussing. EC-I-PLA2 was non-lethal to mice and devoid of neurotoxicity, myotoxicity, anticoagulant activity and cytotoxicity. It induced mild oedema in the foot pads of mice. The purified PLA2 inhibited ADP, collagen and epinephrine induced human platelet aggregation and the inhibition was both dose and time dependent.
Kemparaju
K
K
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Krishnakanth
T P
TP
Veerabasappa Gowda
T
T
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002850:Chromatography, Gel; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010741:Phospholipases A / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D054467:Phospholipases A2; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D014757:Viper Venoms / Q000201:enzymology*; D017819:Viperidae
D010975:Platelet Aggregation Inhibitors; D014757:Viper Venoms; D000244:Adenosine Diphosphate; D003094:Collagen; D010741:Phospholipases A; D054467:Phospholipases A2; D004837:Epinephrine