The reduced expression of 6Ckine in the plt mouse results from the deletion of one of two 6Ckine genes.
6Ckine is an unusual chemokine capable of attracting naive T lymphocytes in vitro. It has been recently reported that lack of 6Ckine expression in lymphoid organs is a prominent characteristic of mice homozygous for the paucity of lymph node T cell (plt) mutation. These mice show reduced numbers of T cells in lymph nodes, Peyer's patches, and the white pulp of the spleen. The genetic reason for the lack of 6Ckine expression in the plt mouse, however, has remained unknown. Here we demonstrate that mouse 6Ckine is encoded by two genes, one of which is expressed in lymphoid organs and is deleted in plt mice. A second 6Ckine gene is intact and expressed in the plt mouse.
Vassileva
G
G
Department of Immunology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA.
Soto
H
H
Zlotnik
A
A
Nakano
H
H
Kakiuchi
T
T
Hedrick
J A
JA
Lira
S A
SA
D000818:Animals; D015139:Blotting, Southern; D054421:Chemokine CCL21; D018925:Chemokines / Q000235:genetics; D019742:Chemokines, CC / Q000235:genetics*; D003001:Cloning, Molecular; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000276:immunology; D018390:Gene Targeting; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D013601:T-Lymphocytes / Q000378:metabolism
C515479:Ccl21c protein, mouse; D054421:Chemokine CCL21; D018925:Chemokines; D019742:Chemokines, CC