Detection of alpha-L fucose containing carbohydrates in mouse immature olfactory neurons.
The cellular location of alpha-L fucose was studied in mice olfactory epithelium (OE) using the Ulex europaeus agglutinin-I (UEA-I) and Tetragonolobus purpureas agglutinin (TPA). In adult mice, UEA-I and TPA revealed a layer of cells that mostly correspond to immature olfactory neurons. Moreover, autoradiographic studies after 3H-T incorporation showed that UEA-I cell labelling occurred during the week following the division of neural cell precursors. In newborn animals, active neurogenesis process is associated with a higher number of UEA-I labelled cells. Olfactory neurogenesis may thus involve a transient event of protein fucosylation, preceding axonal growth.
Ducray
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Laboratoire de Neurosciences, EA 1063, Université de Franche-Comté, Besançon, France. aducray@caramail.com
Propper
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Kastner
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D000818:Animals; D000831:Animals, Newborn; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D005643:Fucose / Q000032:analysis* / Q000737:chemistry; D006001:Glycoconjugates / Q000032:analysis; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009831:Olfactory Mucosa / Q000737:chemistry*; D018034:Olfactory Receptor Neurons / Q000737:chemistry*; D037121:Plant Lectins
D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C036554:dolichos biflorus agglutinin; D005643:Fucose