Cryo-techniques applied to stratum corneum with description of a new sample holder for cryo-scanning electron microscopy of freeze-fractured samples.
Stratum corneum structure greatly differs from that of the living epidermis and specific sample cryo-preparation techniques have to be used. Practical aspects of these cryo-techniques applied to stratum corneum are discussed. Emphasis is placed on scanning electron microscopy of cryo-fixed samples. A new sample holder designed for cryo-scanning electron microscopy of freeze-fractured stratum corneum is described.
Hallegot
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P
L'Oréal Recherche, Department of Analytical Chemistry, 1 av. Eugène Schueller, BP22, 93601 Aulnay-sous-Bois Cedex, France. phallegot@loreal-recherche.com
Minondo
A M
AM
Fiat
F
F
D020285:Cryoelectron Microscopy / Q000295:instrumentation*; D004817:Epidermis / Q000648:ultrastructure*; D005614:Freeze Fracturing; D017110:Freeze Substitution; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation*; D016628:Replica Techniques