Knockout of the Sendai virus C gene eliminates the viral ability to prevent the interferon-alpha/beta-mediated responses.
Sendai virus (SeV) renders cells unresponsive to interferon (IFN)-alpha. To identify viral factors involved in this process, we examined whether recombinant SeVs, which could not express V protein, subsets of C proteins (C, C', Y1 and Y2) or any of four C proteins, retained the capability of impeding IFN-alpha-mediated responses. Among these viruses, only the 4C knockout virus completely lost the ability to suppress the induction of IFN-alpha-stimulated gene products and the subsequent establishment of an anti-viral state. These findings reveal crucial roles of the SeV C proteins in blocking IFN-alpha-mediated responses.
Gotoh
B
B
Department of Microbiology, Fukui Medical University, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui, Japan. bin@fmsrsa.fukui-med.ac.jp
Takeuchi
K
K
Komatsu
T
T
Yokoo
J
J
Kimura
Y
Y
Kurotani
A
A
Kato
A
A
Nagai
Y
Y
D000998:Antiviral Agents / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D017353:Gene Deletion; D050832:Interferon-Stimulated Gene Factor 3; D016898:Interferon-alpha / Q000494:pharmacology*; D016899:Interferon-beta / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D018108:Respirovirus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis
D000998:Antiviral Agents; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D050832:Interferon-Stimulated Gene Factor 3; D016898:Interferon-alpha; D014157:Transcription Factors; C020723:citrate-binding transport protein; D016899:Interferon-beta