title: Genetic interactions between error-prone and error-free postreplication repair pathways in Saccharomyces cerevisiae.
abstract: Evidence obtained from recent studies supports the existence of an error-free postreplication repair (PRR) and a mutagenesis pathway within the Saccharomyces cerevisiae RAD6 DNA repair group. The MMS2 gene is the only known yeast gene involved in error-free PRR that, when mutated, significantly increases the spontaneous mutation rate. In this study, the mutational spectrum of the mms2 mutator was determined and compared to the wild type strain. In addition, mutagenenic effects and genetic interactions of the mms2 mutator and rev3 anti-mutator were examined with respect to forward mutations, frameshift reversions as well as amber and ochre suppressions. It was concluded from these results that the mms2 mutator phenotype is largely dependent on the functional REV3 gene. The synergistic effects of mms2 and rev3 mutations towards killing by a variety of DNA-damaging agents ruled out the possibility that MMS2 simply acts to suppress REV3 activity and favored the hypothesis that MMS2 and REV3 form two alternative subpathways within the RAD6 DNA repair pathway. Taken together, we propose that two pathways represented by MMS2 and REV3 deal with a similar range of endogenous and environmental DNA damage but with different biological consequences, namely, error-free repair and mutagenesis, respectively.
authors.affiliation: Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada. xiaow@sask.usask.ca
authors.forename: W
authors.initials: W
authors.lastname: Xiao
authors.forename: B L
authors.initials: BL
authors.lastname: Chow
authors.forename: T
authors.initials: T
authors.lastname: Fontanie
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: S
authors.initials: S
authors.lastname: Bacchetti
authors.forename: T
authors.initials: T
authors.lastname: Hryciw
authors.forename: S
authors.initials: S
authors.lastname: Broomfield
mesh_terms: D001483:Base Sequence; D004260:DNA Repair / Q000235:genetics*; D004261:DNA Replication; D004271:DNA, Fungal; D004259:DNA-Directed DNA Polymerase; D005656:Fungal Proteins / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C112520:MMS2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases; D004259:DNA-Directed DNA Polymerase; C092602:REV3 protein, S cerevisiae