Evaluation of two automated enzyme-immunoassays for detection of thermophilic campylobacters in faecal samples from cattle and swine.
We evaluated the performance of two enzyme-immunoassays (EIA) for the detection of naturally occurring, thermophilic Campylobacter spp. found in faecal samples from cattle (n = 21 and n = 26) and swine (n = 43) relative to the standard culture method, and also assuming that none of the tests was the definitive standard. The primary isolation both for the culture and the EIA methods was carried out by overnight selective enrichment in Preston broth. The results showed good sensitivities for both EIA methods in cattle (95% and 84%) and swine (88% and 69%) samples. However, when testing cattle samples, EIA-2 method resulted in a rather low specificity (32%). This seemed to be partially due to the isolation of nonthermophilic species. In conclusion, EIA-1 method may provide a simple and fast tool with good accuracy in cattle and swine samples for automated screening of large number of samples.
Hoorfar
J
J
Danish Veterinary Laboratory, Copenhagen, Denmark. jho@svs.dk
Nielsen
E M
EM
Stryhn
H
H
Andersen
S
S
D000818:Animals; D001322:Autoanalysis; D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D002417:Cattle / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006358:Hot Temperature; D007124:Immunoenzyme Techniques / Q000379:methods; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*