Distribution of gamma-tubulin in multipolar spindles and multinucleated cells induced by dimethylarsinic acid, a methylated derivative of inorganic arsenics, in Chinese hamster V79 cells.
Localization of gamma-tubulin, a well-characterized component of microtubule-organizing centers (MTOCs), was investigated because of interest in the mechanism of the induction of aberrant mitotic spindles in Chinese hamster V79 cells exposed to dimethylarsinic acid (DMAA). In control cultures, gamma-tubulin in interphase cells was located as a perinuclear dot on which the microtubules were nucleated. In metaphase cells, the location of gamma-tubulin coincided with that of the mitotic spindle poles. DMAA caused mitotic delay and aberrant spindles, such as tripolar- and quadripolar spindles, in the mitotic cells. Gamma-tubulin was co-localized with the aberrant spindles induced by DMAA. The incidence of gamma-tubulin in the mitotic cells coincided with that of the aberrant spindles and rose with an increasing concentration of DMAA. By contrast, DMAA did not influence the number and location of gamma-tubulin signals in interphase cells. These results suggest that multiple microtubule nucleation sites were induced by DMAA during transition from interphase to mitotic phase. DMAA-induced multiple signals of gamma-tubulin were integrated into one signal at the center of multinucleated cells, surrounded by multiple nuclei as the cell cycle progressed to the next interphase, suggesting the presence of a self-integration mechanism of centrosomal MTOCs during the cell cycle.
Ochi
T
T
Department of Toxicology and Environmental Health, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, Japan. tkfmochi@pharm.teikyo-u.ac.jp
Nakajima
F
F
Nasui
M
M
D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D016466:CHO Cells; D002101:Cacodylic Acid / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects; D018385:Centrosome / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D003703:Demecolcine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin; D002101:Cacodylic Acid; D001151:Arsenic; D003703:Demecolcine