title: Evaluation of AFLP, a high-resolution DNA fingerprinting method, as a tool for molecular subtyping of enterohemorrhagic Escherichia coli O157:H7 isolates.
abstract: The amplified fragment-length polymorphism (AFLPTM) technique is based on the selective PCR amplification of restriction fragments. We investigated the utility of AFLP in the molecular subtyping of enterohemorrhagic Escherichia coli serotype O157:H7 isolates. We analyzed a total of 46 isolates of E. coli O157:H7 along with other serotypes, O26:H11, 0114:H19 and 0119:NT. Isolates of E. coli O157:H7 derived from the same outbreak showed an identical AFLP-banding pattern and were subtyped into the same group, giving results almost consistent with those of a pulsed-field gel electrophoresis (PFGE) study, while other serotypes showed clearly different patterns from those of E. coli O157:H7. These results suggest that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E. coli O157:H7.
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iyoda
authors.forename: A
authors.initials: A
authors.lastname: Wada
authors.forename: J
authors.initials: J
authors.lastname: Weller
authors.forename: S J
authors.initials: SJ
authors.lastname: Flood
authors.forename: E
authors.initials: E
authors.lastname: Schreiber
authors.forename: B
authors.initials: B
authors.lastname: Tucker
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific