Isolation of Sarcocystis falcatula from the South American opossum (Didelphis albiventris) from Argentina.
Sarcocystis sporocysts from the intestines of four opossums (Didelphis albiventris) from Argentina were identified as Sarcocystis falcatula based on schizogonic stages and pathogenicity to budgerigars (Melopsittacus undulatus). Seven budgerigars fed sporocysts from the opossum feces died of acute sarcocystosis 8, 9, 11, 12, and 14 days after inoculation. Schizonts and merozoites found in the lungs and other organs of the budgerigars were identified as S. falcatula based on structure and immunoreactivity with S. falcatula-specific antibody. Sarcocystis falcatula was also isolated in bovine monocyte cell cultures inoculated with lung tissue from a budgerigar that died nine days after ingesting sporocysts. Two budgerigars inoculated subcutaneously with 1,000,000 culture-derived S. falcatula died 11 and 12 days post-inoculation. This is the first report of S. falcatula infection in South America.
Dubey
J P
JP
United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, MD 20705-2350, USA. jdubey@lpsi.barc.usda.gov
Venturini
L
L
Venturini
C
C
Basso
W
W
Unzaga
J
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000276:immunology; D001118:Argentina; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009893:Opossums / Q000469:parasitology*; D010311:Parrots / Q000469:parasitology*; D011817:Rabbits; D012522:Sarcocystis / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D012523:Sarcocystosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*
D000913:Antibodies, Protozoan