Detection of early developmental stages of Myxobolus cerebralis in fish and tubificid oligochaete hosts by in situ hybridization.
The myxosporean and actinosporean spores of Myxobolus cerebralis develop through many stages in their respective hosts, salmonid fishes and a tubificid oligochaete. Using a modified, non-radioactive in situ hybridization protocol, the parasite, which exhibits radically different structural forms during its development in each host, could be specifically detected in paraffin-embedded tissues of both fish and oligochaetes. Our study aims to demonstrate the application of the technique for detection of early stages of M. cerebralis in both hosts.
Antonio
D B
DB
School of Veterinary Medicine, Department of Medicine and Epidemiology, University of California, Davis 95616, USA.
El-Matbouli
M
M
Hedrick
R P
RP
D000818:Animals; D056890:Eukaryota / Q000254:growth & development* / Q000302:isolation & purification; D005393:Fish Diseases / Q000469:parasitology*; D006790:Host-Parasite Interactions; D017403:In Situ Hybridization; D009835:Oligochaeta / Q000469:parasitology*; D017686:Oncorhynchus mykiss / Q000469:parasitology*; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms