Cytotoxic activity and cytokine gene induction of Asp-hemolysin to murine macrophages.
We examined the effects of Asp-hemolysin from Aspergillus fumigatus Fresenius-Muramatsu strain on the viability and cytokine gene expression of mouse peritoneal macrophages. The cytotoxic activity of Asp-hemolysin to macrophages cultured in FCS-RPMI medium was increased in a dose-dependent manner. Treatment of Asp-hemolysin with N-ethylmaleimide or sulfo-N-hydroxy-sulfosuccinimide-acetate caused a remarkable loss of the cytotoxic activity, however, the cytotoxic activity of Asp-hemolysin to macrophages cultured in serum-free medium was significantly increased as compared with that in FCS-RPMI medium. As other biological activities of Asp-hemolysin, tumor necrosis factor-alfa (TNF-alfa), interleukin-1 beta(IL-l beta) and interleukin-1 alfa (IL-l alfa) mRNA expression were observed in macrophages cultured with 1 micro g/ml of Asp-hemolysin.
Kumagai
T
T
The First Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1, Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
Nagata
T
T
Kudo
Y
Y
Fukuchi
Y
Y
Ebina
K
K
Yokota
K
K
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics*; D005656:Fungal Proteins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006460:Hemolysin Proteins / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
C089497:Asp-HS protein, Aspergillus fumigatus; D016207:Cytokines; D005656:Fungal Proteins; D006460:Hemolysin Proteins