title: Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection.
abstract: OBJECTIVE
  To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Infertility clinic and molecular genetics laboratory.
  
  
  METHODS
  Ten patients with severe OAT.
  
  
  METHODS
  Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.
  
  
  METHODS
  Gametic aneuploidy was scored in sperm fractions separated by the swim-up technique and clinical results after intracytoplasmic sperm injection were tabulated.
  
  
  RESULTS
  In all cases, chromosome aneuploidy levels in patients were significantly greater than in controls. The type and percentage of aneuploid sperm for all patients with OAT found in both swim-up and pellet fractions were not different, with the exception of diploid sperm, which remained in the pellet fraction. After ET, 2 (20%) of 10 couples achieved successful pregnancies.
  
  
  CONCLUSIONS
  The data show significantly higher rates of diploidy, autosomal disomy and nullisomy, sex chromosome disomy and nullisomy, and total aneuploidy in sperm from all separated fractions obtained from all patients with OAT versus controls. This patient population with OAT may be at increased risk of producing aneuploid offspring.
authors.affiliation: Center for Pediatric Research, Children's Hospital of the King's Daughters, Eastern Virginia Medical School, Norfolk, USA.
authors.forename: J
authors.initials: J
authors.lastname: Pfeffer
authors.forename: M G
authors.initials: MG
authors.lastname: Pang
authors.forename: S F
authors.initials: SF
authors.lastname: Hoegerman
authors.forename: C J
authors.initials: CJ
authors.lastname: Osgood
authors.forename: M W
authors.initials: MW
authors.lastname: Stacey
authors.forename: J
authors.initials: J
authors.lastname: Mayer
authors.forename: S
authors.initials: S
authors.lastname: Oehninger
authors.forename: W G
authors.initials: WG
authors.lastname: Kearns
mesh_terms: D000782:Aneuploidy; D004247:DNA / Q000032:analysis; D004171:Diploidy; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008845:Microinjections; D009845:Oligospermia / Q000235:genetics*; D009865:Oocytes / Q000648:ultrastructure; D011247:Pregnancy; D011446:Prospective Studies; D012307:Risk Factors; D012661:Semen / Q000737:chemistry*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities*; D014019:Tissue Donors
chemical_list: D004247:DNA