title: The vascular profile of regenerative and dysplastic nodules of the cirrhotic liver: implications for diagnosis and classification.
abstract: We investigated the angiogenic phenotype of regenerative and dysplastic hepatocellular nodules to assess whether these lesions have distinct vascular profiles compared with the adjacent nonneoplastic or malignant liver. Forty-three liver nodules surgically removed from 18 patients were classified into regenerative and dysplastic categories. Serial sections of each nodule, adjacent cirrhotic liver (16 patients), and associated hepatocellular carcinoma (HCC) (6 patients), have been immunostained against CD31 and alpha-smooth muscle actin (alphaSMA) to detect capillary and muscular vessels. The study included 20 large regenerative nodules (LRNs), 13 low-grade dysplastic nodules (LGDNs), and 10 high-grade dysplastic nodules (HGDNs). The number of both capillary units and unpaired arteries was significantly increased in HGDNs and malignant lesions over LGDNs, regenerative, and cirrhotic nodules (P <.01), which showed an overlapping vascular profile. In addition, the number of capillary units, but not that of unpaired arteries, was significantly increased in HCC compared with HGDNs (P <.01). These results show that certain angiogenic features segregate HGDNs from other nonmalignant nodules such as LRNs and LGDNs. The former group of lesions is similar to HCC whereas the latter group is undistinguishable from the adjacent cirrhosis as far as their vascular profile is concerned. The adopted investigative approach does not support the morphological distinction between LRNs and LGDNs although it suggests that HGDNs are likely advanced precursors of HCC. An abnormal number of capillary units and/or unpaired arteries in a nonmalignant hepatocellular nodule can be diagnostically helpful to identify a precancerous lesion.
authors.affiliation: Departments of Pathology, Humanitas Clinical Institute of Rozzano, Milan, Italy. massimo.roncalli@humanitas.it
authors.forename: M
authors.initials: M
authors.lastname: Roncalli
authors.forename: E
authors.initials: E
authors.lastname: Roz
authors.forename: G
authors.initials: G
authors.lastname: Coggi
authors.forename: M G
authors.initials: MG
authors.lastname: Di Rocco
authors.forename: P
authors.initials: P
authors.lastname: Bossi
authors.forename: E
authors.initials: E
authors.lastname: Minola
authors.forename: M
authors.initials: M
authors.lastname: Gambacorta
authors.forename: M
authors.initials: M
authors.lastname: Borzio
mesh_terms: D000199:Actins / Q000032:analysis; D002196:Capillaries / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000473:pathology*; D005260:Female; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000473:pathology*; D008103:Liver Cirrhosis / Q000145:classification* / Q000473:pathology*; D008113:Liver Neoplasms / Q000098:blood supply / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis
chemical_list: D000199:Actins; D019408:Platelet Endothelial Cell Adhesion Molecule-1