title: Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice.
abstract: Early life responses to respiratory syncytial virus (RSV)-F DNA and RSV-F protein immunization were studied in murine models of neonatal immunization. RSV-F DNA induced similar antibody (Ab) responses, antigen-specific IFN-gamma production and cytotoxic T lymphocyte (CTL) responses in 1-week-old and adult BALB / c mice. In contrast, RSV-F protein induced much higher IL-5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV-F DNA priming / protein boosting primed 1-week-old mice for RSV-F-specific CTL responses, reduced IL-5 production and enhanced Ab responses. In contrast, IL-5 exceeded IFN-gamma responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL-5 responses but strong CTL responses. Sequential or combined DNA / protein immunization thus represent interesting strategies for early life immunization.
authors.affiliation: W.H.O. Collaborating Center for Neonatal Vaccinology Departments of Pathology and Pediatrics, University of Geneva Medical School, Geneva, Switzerland.
authors.forename: X
authors.initials: X
authors.lastname: Martinez
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Kovarik
authors.forename: M
authors.initials: M
authors.lastname: Klein
authors.forename: P H
authors.initials: PH
authors.lastname: Lambert
authors.forename: C A
authors.initials: CA
authors.lastname: Siegrist
mesh_terms: D000818:Animals; D015707:HN Protein; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D013997:Time Factors; D017778:Vaccines, Combined / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015707:HN Protein; D007074:Immunoglobulin G; D017778:Vaccines, Combined; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C116204:attachment protein G