Sustained performance of knowledge-based potentials in fold recognition.
We describe the results obtained using fold recognition techniques in our third participation in the CASP experiment. The approach relies on knowledge-based potentials for alignment production and fold identification. As indicated by the increase in alignment quality and fold identification reliability, the predictions improved from CASP1 to CASP3. In particular, we identified structural relationships in which no known evolutionary link exists. Our predictions are based on single sequences rather than multiple sequence alignments. Additionally, we voluntarily submitted only a single model for each target because, in our view, submission of a single model is the most stringent test. We describe the methods used, the strategy adopted in the predictions, and the prediction results and discuss future work.
Domingues
F S
FS
Center for Applied Molecular Engineering, University of Salzburg, Austria.
Koppensteiner
W A
WA
Jaritz
M
M
Prlic
A
A
Weichenberger
C
C
Wiederstein
M
M
Floeckner
H
H
Lackner
P
P
Sippl
M J
MJ
D000465:Algorithms; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D019757:Carbon-Oxygen Lyases / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D016208:Databases, Factual; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012269:Ribosomal Proteins / Q000737:chemistry; D016415:Sequence Alignment
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C080002:MarA protein, E coli; D011506:Proteins; D012269:Ribosomal Proteins; D019757:Carbon-Oxygen Lyases; C021578:methylglyoxal synthase