A transient, neuron-wide form of CREB-mediated long-term facilitation can be stabilized at specific synapses by local protein synthesis.
In a culture system where a bifurcated Aplysia sensory neuron makes synapses with two motor neurons, repeated application of serotonin (5-HT) to one synapse produces a CREB-mediated, synapse-specific, long-term facilitation, which can be captured at the opposite synapse by a single pulse of 5-HT. Repeated pulses of 5-HT applied to the cell body of the sensory neuron produce a CREB-dependent, cell-wide facilitation, which, unlike synapse-specific facilitation, is not associated with growth and does not persist beyond 48 hr. Persistent facilitation and synapse-specific growth can be induced by a single pulse of 5-HT applied to a peripheral synapse. Thus, the short-term process initiated by a single pulse of 5-HT serves not only to produce transient facilitation, but also to mark and stabilize any synapse of the neuron for long-term facilitation by means of a covalent mark and rapamycin-sensitive local protein synthesis.
Casadio
A
A
Howard Hughes Medical Institute and Center for Neurobiology and Behavior College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Martin
K C
KC
Giustetto
M
M
Zhu
H
H
Chen
M
M
Bartsch
D
D
Bailey
C H
CH
Kandel
E R
ER
D000818:Animals; D001048:Aplysia / Q000502:physiology*; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008568:Memory / Q000502:physiology; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis; D012701:Serotonin / Q000494:pharmacology* / Q000502:physiology; D020123:Sirolimus / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology*
D017362:Cyclic AMP Response Element-Binding Protein; D009419:Nerve Tissue Proteins; D003609:Dactinomycin; D012701:Serotonin; D020123:Sirolimus