Neutrophil chemotaxis on silicone and polyurethane surfaces.
Silicone vascular catheters have a greater risk of infection and produce greater inflammation in vivo and greater complement activation in vitro than other vascular catheter polymer materials. This study investigated whether polymorphonuclear leukocyte (PMNL) chemotaxis under agarose on silicone surfaces is different than on polyurethane (PU). Glass slides were coated with silicone and PU by use of a constant-speed dipping apparatus. Chemotaxis (3 h) in response to (10-7 mL) FMLP, zymosan-activated serum, and fresh serum (100%) was greater on silicone than on PU (P<.05). Polyclonal antibody to C5a blocked >50% of the movement toward serum (P<.05). Serum in the PMNL well significantly decreased chemotaxis toward FMLP on silicone (P<.05) but not on PU. These findings suggest that excessive complement activation by silicone may interfere with chemotaxis, but further work is necessary to determine whether this is relevant to an increased risk of catheter-related infection.
Indorf
A S
AS
Section on Infectious Diseases, Department of Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA.
Poate
T
T
Sherertz
R J
RJ
D001769:Blood; D002404:Catheterization / Q000295:instrumentation; D002634:Chemotaxis, Leukocyte; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D011140:Polyurethanes; D012828:Silicones; D013499:Surface Properties; D015054:Zymosan / Q000494:pharmacology
D011140:Polyurethanes; D012828:Silicones; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan