L-selectin and leukocyte function in skeletal muscle reperfusion injury.
OBJECTIVE
Treatment with anti-L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle.


METHODS
Prospective, randomized experimental trials.


METHODS
Basic science research laboratory.


METHODS
Male Wistar rats weighing 109 +/- 5 g (mean +/- SEM).


METHODS
Gracilis pedicle muscle flaps were elevated and microcirculation was observed by intravital microscopy. Two groups were evaluated: (1) the control group, which received 4 hours of global ischemia, and (2) the experimental group, which received 4 hours of global ischemia, plus treatment with anti-L-selectin monoclonal antibody 30 minutes before reperfusion.


METHODS
The number of rolling and adherent leukocytes in postcapillary venules were counted in the 2 groups at baseline and at 1 through 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion.


RESULTS
Treatment with the monoclonal antibody to L-selectin significantly reduced the number of rolling leukocytes (flux) at 2 through 5, 20, 30, 45, and 60 minutes of reperfusion compared with controls (P<.05). Use of the monoclonal antibody significantly reduced the number of adherent neutrophils at 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion (P<.05). There was no significant difference in leukocyte velocity.


CONCLUSIONS
L-Selectin plays a significant role in leukocyte rolling and adherence to venular endothelium in rat skeletal muscle ischemia reperfusion injury.
Lozano
D D
DD
Division of Plastic Surgery, Microsurgery and Hyperbaric Laboratory, School of Medicine, University of Nevada, Las Vegas 89102, USA.
Kahl
E A
EA
Wong
H P
HP
Stephenson
L L
LL
Zamboni
W A
WA
D000818:Animals; D019041:L-Selectin / Q000276:immunology*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply*; D009504:Neutrophils / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000276:immunology*
D019041:L-Selectin