Genomic organization of the Oreochromis mossambicus glutamate receptor subunit 2 beta gene (fGluR2 beta): presence of two different 5'-untranslated regions.
The AMPA (alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid)-preferring receptor is one of the pharmacologically defined ionotropic glutamate receptors, which mediate fast excitatory synaptic transmission in the central nervous system of vertebrates. Here, we report the mapping of the transcriptional start points and identification of the intron-exon boundaries of the teleost AMPA receptor subunit gene fGluR2 beta. fGluR2 beta and the mouse GluR2 share a similar genomic organization, having identical intron insertion sites and a large intron 2; however, fGluR2 beta has an extra exon encoding an alternate 5'-UTR. Results of RT-PCR and RNase protection analyses indicate that mature fish brain expresses two types of fGluR2 beta transcripts with different 5' ends. Transcriptions of these two fGluR2 beta transcripts started from two chromosomal regions separated by at least 10 kb. Only the transcript starting from the region more upstream on the chromosome was spliced. Moreover, transcript initiated from the downstream region was more abundant than that initiated from the upstream region.
Chen
Y C
YC
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan.
Kung
S S
SS
Wu
Y M
YM
Huang
C J
CJ
Chow
W Y
WY
D020121:5' Untranslated Regions; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D005091:Exons; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017393:RNA Editing; D012323:RNA Processing, Post-Transcriptional; D018091:Receptors, AMPA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D017210:Tilapia / Q000235:genetics*; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D017931:DNA Primers; D018091:Receptors, AMPA; D012260:Ribonucleases; C104722:glutamate receptor ionotropic, AMPA 2