Histochemical localization of reverse transcriptase in polytene chromosomes of chironomids.
The localization of a reverse transcriptase-related protein in salivary gland polytene chromosomes was investigated by immunohistochemistry in two species of Chironomus. The antibodies used were raised against a recombinant protein containing phylogenetically conserved motifs of reverse transcriptases and derived from an abundant non-LTR element previously identified in Chironomus. Immunoreactive protein was found in some telomeres, in a centromeric region, in a few interstitial bands and in Balbiani ring 3. The telomeric signal was probably dependent on transcription and increased dramatically when telomeric heat shock puffs were induced. A correlation with transcription was also seen in Balbiani ring 3, the immunobinding of which disappeared after inhibition of transcription with actinomycin D.
López
C C
CC
Department of Genetics, University of Lund, Sölvegatan 29, S-22362 Lund, Sweden.
Rodriguez
E
E
Díez
J L
JL
Edström
J
J
Morcillo
G
G
D000818:Animals; D002503:Centromere / Q000201:enzymology / Q000648:ultrastructure; D002683:Chironomidae / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D002875:Chromosomes / Q000201:enzymology*; D003609:Dactinomycin / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D019476:Insect Proteins / Q000032:analysis* / Q000276:immunology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D018626:Retroelements / Q000235:genetics*; D012469:Salivary Glands / Q000648:ultrastructure; D016615:Telomere / Q000201:enzymology / Q000648:ultrastructure; D014158:Transcription, Genetic / Q000187:drug effects
D007074:Immunoglobulin G; D019476:Insect Proteins; D011993:Recombinant Fusion Proteins; D018626:Retroelements; D003609:Dactinomycin; D012194:RNA-Directed DNA Polymerase