Assessment of a magnetic bead-EIA based kit for rapid diagnosis of fish pasteurellosis.
The accuracy of the magnetic beads-EIA based BIONOR AQUAEIA-Pp kit for the rapid diagnosis of pasteurellosis was evaluated. The kit reacted with all the Photobacterium damselae subsp. piscicida strains included in this study, with a detection limit of 10(4) bacteria/ml. However, non-specific reactions were observed with isolates of Ph. damselae subsp. damselae or Ph. histaminum when the bacterial concentration was high (10(9)-10(10) bacteria/ml). Similar findings in specificity and sensitivity were observed when the kit was applied to experimentally infected fish tissues. However, since those bacterial species are not usually found in the fish species susceptible to pasteurellosis, the AQUAEIA kit appears applicable for a rapid screening of the disease. In addition, when the kit was utilized to analyze cultured populations of seabream, it allowed the detection of the pathogen, not only in individuals affected by the disease, but also in asymptomatic carrier fish. Furthermore, the positive detection of Ph. damselae subsp. piscicida in broodstock gonads, seminal, and ovaric fluids, and also in eggs indicated the possibility of vertical transmission of pasteurellosis.
Romalde
J L
JL
Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago de Compostela, Spain. mpromald@ucs.es
Magariños
B
B
Lores
F
F
Osorio
C R
CR
Toranzo
E
E
D000818:Animals; D002353:Carrier State / Q000175:diagnosis / Q000662:veterinary*; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology*; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D010776:Photobacterium / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012623:Seawater / Q000382:microbiology; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic