Crystallization and preliminary diffraction analysis of the HincII restriction endonuclease-DNA complex.
Crystals of the 60 kDa dimeric HincII restriction enzyme bound to a 12 base-pair dyad-symmetric duplex DNA carrying the specific 5'-GTCGAC recognition site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as precipitating agent. The rod-shaped crystals belong to space group I222 (or I2(1)2(1)2(1)), with unit-cell dimensions a = 66.9, b = 176.7, c = 256.0 A. There are most likely to be two dimeric complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.5 A at 100 K, with an R(merge) of 4.8%.
Horton
N C
NC
Department of Chemistry, Interdepartmental Program in Biochemistry and Molecular Biology, University of California at Santa Barbara, Santa Barbara CA 93106-9510, USA.
Dorner
L F
LF
Schildkraut
I
I
Perona
J J
JJ
D001426:Bacterial Proteins / Q000737:chemistry; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D019281:Dimerization; D004926:Escherichia coli; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011092:Polyethylene Glycols; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D009838:Oligodeoxyribonucleotides; D011092:Polyethylene Glycols; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases