Tissue distribution of cadmium in rats given minimum amounts of cadmium-polluted rice or cadmium chloride for 8 months.
To investigate the relationship between cadmium (Cd) toxicity, intestinal absorption, and its distribution to various tissues in rats treated orally with minimum amounts of Cd, 14 female rats per dose group per time point were given diets consisting of 28% purified diet and 72% ordinary rice containing Cd-polluted rice (0. 02, 0.04, 0.12, or 1.01 ppm of Cd) or CdCl(2) (5.08, 19.8, or 40.0 ppm of Cd) for up to 8 months. At 1, 4, and 8 months after the commencement of Cd treatment, seven rats per group were euthanized for pathological examinations to determine the Cd concentrations in the liver and kidneys and metallothionein (MT) in the liver, kidneys, intestinal mucosa, serum, and urine. One week before each period of 1, 4, and 8 months, the remaining seven rats in each group were administered a single dosage of (109)Cd, a tracer, to match the amounts of the designated Cd doses (about 1.2 to 2400 microg/kg body wt). They were euthanized 5 days later to determine the distribution of Cd to various tissues. No Cd-related toxic changes were observed. The concentrations of Cd in the liver and kidneys at any time point and MT in the liver, kidney, serum, and urine at 4 and 8 months increased dose-dependently, whereas MT in the intestinal mucosa did not alter markedly at any time point. The distribution rates of Cd to the liver increased dose-dependently (40% at lower doses to 60% at higher doses), whereas those to the kidney decreased dose-dependently (20% at lower doses to 10% at higher doses). The Cd retention rates 5 days after (109)Cd administration (amounts of Cd in various tissues/amounts of Cd administered) ranged from 0.2 to 1. 0% at any time point. These results suggest that the distribution of Cd to the liver and kidneys after the oral administration vary depending on the dosage levels of Cd. The difference of the distribution pattern of Cd to the liver and kidney is probably due to the difference in the form of the absorbed Cd, i.e., free ion or Cd-MT complex, although not closely related to the MT in the intestinal mucosa.
Hiratsuka
H
H
Mitsubishi Chemical Safety Institute Ltd., 14 Sunayama, Hasaki, Kashima, Ibaraki, 314-0255, Japan.
Satoh
S i
Si
Satoh
M
M
Nishijima
M
M
Katsuki
Y
Y
Suzuki
J
J
Nakagawa
J i
Ji
Sumiyoshi
M
M
Shibutani
M
M
Mitsumori
K
K
Tanaka-Kagawa
T
T
Ando
M
M
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight; D002104:Cadmium / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity; D019256:Cadmium Chloride / Q000493:pharmacokinetics; D002106:Cadmium Radioisotopes; D004032:Diet; D005260:Female; D005506:Food Contamination; D008668:Metallothionein / Q000097:blood / Q000378:metabolism / Q000652:urine; D012275:Oryza; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014018:Tissue Distribution
D002106:Cadmium Radioisotopes; D002104:Cadmium; D008668:Metallothionein; D019256:Cadmium Chloride