title: Ovarian dysplasia in prophylactic oophorectomy specimens: cytogenetic and morphometric correlations.
abstract: BACKGROUND
  Ovarian dysplasia, a potential precursor to ovarian carcinoma, has been described in ovarian tissue obtained by prophylactic oophorectomy and also adjacent to ovarian carcinoma. Women with a family history of ovarian carcinoma, especially those of Jewish Ashkenazi descent, often test positive for BRCA mutant genes. Prophylactically removed ovaries, generally described as normal on macroscopic examination, can exhibit a "preneoplastic phenotype" and unsuspected neoplasm.
  
  
  METHODS
  Histologic slides of ovarian tissue from 54 Ashkenazi Jewish women were reviewed. All had a family history of ovarian carcinoma and all were tested for BRCA mutations. Forty-four women tested positive. Thirty-one women underwent prophylactic oophorectomy and 23 underwent oophorectomy for ovarian carcinoma. Normal, dysplastic, and ovarian carcinoma epithelial cells were analyzed morphometrically combining nuclear area measurements with chromatin texture assessment using a novel method based on the computation of autocorrelation coefficients and a derived parameter (Beta). Discriminant analysis between classificatory algorithms was used to obtain results.
  
  
  RESULTS
  Ovarian dysplasia was identified in 77.6% of the prophylactic oophorectomy specimens. An unsuspected ovarian carcinoma was diagnosed in one prophylactic oophorectomy specimen. Of 10 women who underwent prophylactic oophorectomy and were negative for BRCA mutations, three had ovarian dysplasia. The average nuclear measurements of the dysplastic cells were similar to those published previously. The new autocorrelation-based method evaluating nuclear texture, as revealed by tridimensional surface plots, demonstrated high discriminatory potential. Discriminant analysis based on nuclear area and nuclear texture information resulted in the correct classification of nearly all the cases in the three diagnostic categories.
  
  
  CONCLUSIONS
  Ovaries removed by prophylactic oophorectomy examined in their entirety often reveal ovarian dysplasia and occasionally ovarian carcinoma. The new morphometric method used was highly discriminatory in the evaluation of nuclear texture. Ovarian dysplasia in women with risk factors for ovarian carcinoma is significant in early ovarian carcinogenesis.
authors.affiliation: Department of Pathology, The Mount Sinai-NYU Medical Center, New York, New York 10029, USA.
authors.forename: L
authors.initials: L
authors.lastname: Deligdisch
authors.forename: J
authors.initials: J
authors.lastname: Gil
authors.forename: H
authors.initials: H
authors.lastname: Kerner
authors.forename: H S
authors.initials: HS
authors.lastname: Wu
authors.forename: D
authors.initials: D
authors.lastname: Beck
authors.forename: R
authors.initials: R
authors.lastname: Gershoni-Baruch
mesh_terms: D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000235:genetics / Q000473:pathology; D002843:Chromatin / Q000473:pathology; D003205:Computing Methodologies; D020732:Cytogenetic Analysis; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005260:Female; D019398:Genes, BRCA1 / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008875:Middle Aged; D010049:Ovarian Diseases / Q000235:genetics / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology; D010052:Ovariectomy; D010053:Ovary / Q000378:metabolism / Q000473:pathology*
chemical_list: D002843:Chromatin