title: Preparation and characterization of cationic microspheres for gene delivery.
abstract: The production and characterization of cationic microparticles based on Eudragit RS and cationic agents (i.e. a cationic acrylic polymer and three different cationic surfactants) for the delivery of nucleic acids is here described. It was found that morphological and dimensional characteristics of microparticles were influenced by the type and concentration of cationic agent employed and by some experimental parameters such as stirring speed, emulsifying agent and type of rotor. The desoxiribonucleotide Defibrotide (DFT) was associated with positively charged microparticles and its in vitro release kinetics from microparticles were determined. A study of the in vitro toxicity of cationic microparticles on cultured human cell line K562 was also performed, demonstrating that DDAB(18) microparticles display very low cytotoxicity.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Ferrara, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Esposito
authors.forename: S
authors.initials: S
authors.lastname: Sebben
authors.forename: R
authors.initials: R
authors.lastname: Cortesi
authors.forename: E
authors.initials: E
authors.lastname: Menegatti
authors.forename: C
authors.initials: C
authors.lastname: Nastruzzi
mesh_terms: D000180:Acrylic Resins; D002412:Cations / Q000009:adverse effects*; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D005343:Fibrinolytic Agents / Q000008:administration & dosage; D015316:Genetic Therapy; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D008863:Microspheres; D010316:Particle Size; D011089:Polydeoxyribonucleotides / Q000008:administration & dosage
chemical_list: D000180:Acrylic Resins; D002412:Cations; D005343:Fibrinolytic Agents; D011089:Polydeoxyribonucleotides; C050528:Eudragit RS; C036901:defibrotide