Application of complementary DNA microarray technology to carcinogen identification, toxicology, and drug safety evaluation.
One major challenge facing today's cancer researchers and toxicologists is the development of new approaches for the identification of carcinogens and other environmental hazards. Here, we describe the potential impact of emerging technologies for measuring gene expression profiles on carcinogen identification and on the general field of toxicology. An example of one of these technologies is the use of cDNA microarray chips. We provide an overview to the key questions that are confronting investigators charged with determining the relative safety of natural or synthetic chemicals to which humans are exposed, followed by a discussion of how cDNA microarray technology may be applied to these questions. Gene chip technology is still a relatively new technology, and only a handful of studies have demonstrated its utility. However, as the technical hurdles to development are passed, the use of this methodology in addressing the questions raised here will be critical to increase the sensitivity of detection of the potential toxic effects of environmental chemicals and to understand their risks to humans.
Afshari
C A
CA
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
Nuwaysir
E F
EF
Barrett
J C
JC
D002273:Carcinogens / Q000145:classification / Q000633:toxicity*; D003257:Consumer Product Safety; D018076:DNA, Complementary; D015723:Gene Library; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010601:Pharmacology, Clinical / Q000379:methods; D014116:Toxicology / Q000379:methods
D002273:Carcinogens; D018076:DNA, Complementary