title: Phylogenetic analysis of Leptospira strains of pathogenic serovars using 23S rDNA gene sequences.
abstract: The 23S ribosomal DNAs were amplified from 11 strains of Leptospira interrogans sensu lato by polymerase chain reaction (PCR) and sequenced. The PCR products of about 290-bp DNA fragments indicated more than 97% sequence similarity to each other. The phylogenetic tree based on the 23S ribosomal DNAs obtained in this study revealed that 11 strains of L. interrogans examined composed a cluster distinct to that of L. weilii and L. borgpetersenii, confirming that these strains were similar to strain Moulton of L. interrogans serovar canicola in 23S rDNA sequence.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine.
authors.forename: F
authors.initials: F
authors.lastname: Sasaki
authors.forename: R
authors.initials: R
authors.lastname: Kano
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry*; D007919:Leptospira / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S