title: Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout.
abstract: A method was developed for determining benzocaine and N-acetylbenzocaine concentrations in fillet tissue of rainbow trout. The method involves extracting the analytes with acetonitrile, removing lipids or hydrophobic compounds from the extract with hexane, and providing additional clean-up with solid-phase extraction techniques. Analyte concentrations are determined using reversed-phase high-performance liquid chromatographic techniques with an isocratic mobile phase and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., < 14%), and sensitivity (method quantitation limit, < 24 ng/g) for each analyte indicate the usefulness of this method for studies characterizing the depletion of benzocaine residues from fish exposed to benzocaine.
authors.affiliation: US Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, WI 54603, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Meinertz
authors.forename: G R
authors.initials: GR
authors.lastname: Stehly
authors.forename: T D
authors.initials: TD
authors.lastname: Hubert
authors.forename: J A
authors.initials: JA
authors.lastname: Bernardy
mesh_terms: D000818:Animals; D001566:Benzocaine / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D005504:Food Analysis; D017686:Oncorhynchus mykiss; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001566:Benzocaine