title: Differential expression of neutrophil adhesion molecules during coronary artery surgery with cardiopulmonary bypass.
abstract: BACKGROUND
  Activation of neutrophil adhesion molecules and subsequent neutrophil adhesion to vascular endothelium are key events initiating inflammatory organ dysfunction after cardiopulmonary bypass and ischemic reperfusion.
  
  
  OBJECTIVE
  We sought to characterize neutrophil integrin CD11b and L-selectin activation associated with coronary artery bypass graft surgery and to determine whether neutrophil activation contributes to their sequestration on postbypass reperfusion.
  
  
  METHODS
  Twenty patients undergoing routine coronary artery bypass were studied. Heparinized whole blood was simultaneously sampled from a central venous line, aorta, coronary sinus, and right and left atrium before, during, and up to 20 minutes after cardiopulmonary bypass. Neutrophil counts were obtained, and neutrophil CD11b and L-selectin expression was determined by flow cytometric analysis in whole blood.
  
  
  RESULTS
  CD11b expression on circulating neutrophils increased during cardiopulmonary bypass, peaking at 145% of baseline level after release of the aortic clamp and then declined by 20 minutes after bypass (analysis of variance, P =.003). No change in neutrophil L-selectin expression was observed during cardiopulmonary bypass. Neutrophils responded to ex vivo stimulation by C5a and leukotriene B(4) during cardiopulmonary bypass but not at 24 hours after the operation. After reperfusion, neutrophil loss, but not local activation, was demonstrated in the coronary and pulmonary circulations.
  
  
  CONCLUSIONS
  Upregulated CD11b expression on neutrophils is likely to contribute to neutrophil sequestration in the heart and lungs after bypass, but neutrophil activation may be limited by their reduced responsiveness to agonist stimulation. CD11b represents a potential therapeutic target for diminishing inflammation after cardiac operations.
authors.affiliation: Department of Medicine, University of Western Australia, Sir Charles Gairdner Hospital, Perth, Nedlands, Australia.
authors.forename: M K
authors.initials: MK
authors.lastname: Ilton
authors.forename: P E
authors.initials: PE
authors.lastname: Langton
authors.forename: M L
authors.initials: ML
authors.lastname: Taylor
authors.forename: N L
authors.initials: NL
authors.lastname: Misso
authors.forename: M
authors.initials: M
authors.lastname: Newman
authors.forename: P J
authors.initials: PJ
authors.lastname: Thompson
authors.forename: J
authors.initials: J
authors.lastname: Hung
mesh_terms: D018845:CD11 Antigens / Q000096:biosynthesis*; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019041:L-Selectin / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000517:prevention & control; D018375:Neutrophil Activation / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D018845:CD11 Antigens; D019041:L-Selectin