Complement activation in coronary artery bypass grafting patients without cardiopulmonary bypass: the role of tissue injury by surgical incision.
OBJECTIVE
Complement activation is a trigger in inducing inflammation in patients who undergo coronary artery bypass grafting (CABG) and is usually thought to be induced by the use of cardiopulmonary bypass (CPB). In this study, we examined whether tissue injury caused by chest surgical incision per se contributes to complement activation in CABG patients.


METHODS
Prospective study.


METHODS
Thorax center in university hospital.


METHODS
Twenty-two patients undergoing CABG without CPB were prospectively divided into two groups: a small chest incision via an anterolateral thoracotomy representing a minimized tissue injury (lateral group, n = 8), and a conventional median sternotomy representing a large tissue injury (median group, n = 14). Biochemical markers indicating complement activation as well as systemic inflammatory response were determined before, during, and after the operation.


RESULTS
Plasma concentrations of complement 3a increased in both the lateral and median groups right after chest incision (p < 0.01 and p < 0.05, respectively) and by the end of operation increased only in the median group (p < 0.01). The terminal complement complex 5b-9 did not increase in the lateral group, but it did increase in the median group both after incision and by the end of the operation (p < 0.05 and p < 0.05, respectively). During surgery, complement 4a did not increase, suggesting that it is the alternative rather than the classic pathway that is involved in complement activation by tissue injury. Postoperatively, interleukin-6 production was greater in the median group (p < 0.01) than the lateral group (p < 0.05), suggesting a more pronounced inflammatory response to a larger chest incision.


CONCLUSIONS
Tissue injury caused by surgical incision contributes to complement activation in CABG patients who are operated on without CPB. A small anterolateral thoracotomy is associated with reduced complement activation in comparison with a median sternotomy.
Gu
Y J
YJ
Department of Cardiothoracic Surgery, Thorax Center, University Hospital Groningen, The Netherlands.
Mariani
M A
MA
Boonstra
P W
PW
Grandjean
J G
JG
van Oeveren
W
W
D000328:Adult; D000368:Aged; D002315:Cardiopulmonary Bypass; D003167:Complement Activation / Q000276:immunology*; D015926:Complement C3a / Q000378:metabolism; D015934:Complement C4a / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D001026:Coronary Artery Bypass; D005260:Female; D006785:Hospitals, University; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007432:Intraoperative Period; D008297:Male; D008875:Middle Aged; D009426:Netherlands; D011183:Postoperative Complications / Q000276:immunology; D011446:Prospective Studies; D018746:Systemic Inflammatory Response Syndrome / Q000276:immunology*; D013908:Thoracotomy
D015938:Complement Membrane Attack Complex; D015850:Interleukin-6; D015926:Complement C3a; D015934:Complement C4a