Induction of apoptosis by Coprinus disseminatus mycelial culture broth extract in human cervical carcinoma cells.
Extract of Coprinus disseminatus (pers. Fr.) (C. disseminatus) culture broth (EDCB) inhibits proliferation and induces apoptosis in the human cervical carcinoma cells at 5 microg/ml. To determine whether the cell death induced by the EDCB recruits caspases or not, one of the exclusive pathways in cell death, we examined caspase-3 activity in this cell death process. The activity of caspase-3 was remarkably increased when the cell was treated with EDCB, and this activity was nullified by Z-VAD-FMK, a well known caspase-3 inhibitor. From these results, we would expect the EDCB to contain substances with the ability to induce apoptosis in the human cervical carcinoma cells. The extent of the EDCB induced apoptosis is cell line-dependent.
Han
B
B
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Japan. d6b272@edc.cc.gunma-u.ac.jp
Toyomasu
T
T
Shinozawa
T
T
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D017209:Apoptosis; D006690:Bisbenzimidazole / Q000494:pharmacology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D003302:Coprinus / Q000378:metabolism*; D003470:Culture Media; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005456:Fluorescent Dyes / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D000590:Amino Acid Chloromethyl Ketones; D003470:Culture Media; D015853:Cysteine Proteinase Inhibitors; D005456:Fluorescent Dyes; D010936:Plant Extracts; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D006690:Bisbenzimidazole