Cysteine-containing histone H1-like (PL-I) proteins of sperm.
We have determined the presence of cysteine in the protein PL-I from the sperm of the surf clam Spisula solidissima. The existence of cysteine in this histone H1-related protein is responsible for its previously described aggregation behavior. The location of this residue, within the trypsin-resistant domain of the protein, has been established. We have also shown that cysteine is ubiquitously present in the PL-I proteins from the sperm of other bivalve mollusks but is absent from other PL of smaller molecular mass (PL-II, PL-III, PL-IV). We have also found cysteine to be present in the PL-I from a tunicate (Chelysoma productum) but absent in a PL-I from a fish (Mullus barbatus). The possible significance of the unusual occurrence of cysteine in these histone-H1-related proteins is discussed.
Zhang
F
F
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.
Lewis
J D
JD
Ausió
J
J
D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D001974:Bromosuccinimide / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D008297:Male; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017386:Sequence Homology, Amino Acid; D013094:Spermatozoa / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D004268:DNA-Binding Proteins; D006657:Histones; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D013438:Sulfhydryl Compounds; C069634:HOXA10 protein, human; D010450:Endopeptidases; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N; D003545:Cysteine; D001974:Bromosuccinimide