Determination of polyphenols by high-performance liquid chromatography with inhibited chemiluminescence detection.
A chemiluminescence reaction detector was developed for the detection of polyphenols separated by HPLC based on the inhibition of chemiluminescence from the luminol-potassium hexacyanoferrate(III) reaction by polyphenols. The separation was carried out on a RP-C18 column at 37 degrees C by using stepwise gradient elutions. The detection limits are in the range of 6.8 x 10(-7)-2.0 x 10(-9) g/ml for catechol, protocatechuic acid, chlorogenic acid, rutin, resorcinol, hydroquinone and p-tert.butylpyrocatechol. The method is sensitive, selective, fast and simple. It has been successfully applied to the determination of chlorogenic acid and rutin in real tobacco samples.
Cui
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H
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, P.R. China. ccsm@ustc.edu.cn
He
C
C
Zhao
G
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids; D008163:Luminescent Measurements; D010636:Phenols / Q000032:analysis*; D011108:Polymers / Q000032:analysis*; D059808:Polyphenols; D012680:Sensitivity and Specificity
D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols