title: Interleukin-10 is localized to and released by human lung mast cells.
abstract: BACKGROUND
  Mast cells control the local inflammation by producing many kinds of cytokines. Interleukin (IL)-10 is one of the important cytokine that upregulate or downregulate inflammation.
  
  
  OBJECTIVE
  The aim of this study is to ascertain whether IL-10 is produced from human lung mast cells by cross-linkage of high-affinity Fcepsilon receptors (FcepsilonRI).
  
  
  METHODS
  Mast cells were purified using affinity magnetic selection with mAb YB5.B8 (> 93% pure). Mast cells were precultured with human myeloma IgE (3 microg/mL) for 16 h and then washed, and stimulated with anti-IgE in the presence or absence of recombinant human stem cell factor (rhSCF). We have studied the production of IL-10 by using reverse transcription-PCR, enzyme-linked immunosorbent assay and immunocytochemistry.
  
  
  RESULTS
  We found that human lung mast cells were immunocytochemically stained with anti-IL-10 mAb after IgE-dependent stimulation. The activation of mast cells via FcepsilonRI enhanced the intensity of the IL-10 mRNA signal. Anti-IgE (1 microg/mL) induced a median IL-10 release of 301.7 (7.8-1532.4) pg/106 mast cells/24 h. In contrast, mast cells released only a small amount of IL-10 in the absence of anti-IgE. This difference was statistically significant (P = 0.02, n = 11).
  
  
  CONCLUSIONS
  Our findings indicate that human lung mast cells are capable of producing IL-10 in response to IgE-dependent stimulation.
authors.affiliation: The First Department of Internal Medicine, Gunma University, School of Medicine, Maebashi, Gunma, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Okayama
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000737:chemistry / Q000378:metabolism*; D008168:Lung / Q000166:cytology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism*; D019089:Stem Cell Factor / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D000888:Antibodies, Anti-Idiotypic; D019089:Stem Cell Factor; C026408:anti-IgE antibodies; D016753:Interleukin-10; D007073:Immunoglobulin E