title: Cytochrome c-553 from the alkalophilic bacterium Bacillus pasteurii has the primary structure characteristics of a lipoprotein.
abstract: The complete sequence of Bacillus pasteurii cytochrome c-553 was determined by standard methods of Edman degradation of overlapping peptides combined with mass spectrometry. The protein contains 92 residues and a single heme-binding site. It is most similar to Bacillus licheniformis, Bacillus PS3, and Bacillus subtilis cytochromes c-551, which are lipoproteins that are partially solubilized through proteolytic cleavage of the N-terminal diacyl-glyceryl-cysteine membrane anchor. The high yield of the B. pasteurii cytochrome c-553, together with evidence that shorter forms of the cytochrome occur in the mixture of otherwise pure protein, suggests that the membrane anchor is very susceptible to proteolysis and that the soluble form of the cytochrome is therefore released from the membrane upon cell breakage. A sequence-based calculation of the protein secondary structure suggests the presence of a typical cytochrome helical fold with a random-coil N-terminus tail.
authors.affiliation: Laboratorium voor Eiwitbiochemie en Eiwitengineering, University of Gent, Ledeganckstraat 35, Gent, 9000, Belgium.
authors.forename: I H
authors.initials: IH
authors.lastname: Vandenberghe
authors.forename: Y
authors.initials: Y
authors.lastname: Guisez
authors.forename: S
authors.initials: S
authors.lastname: Ciurli
authors.forename: S
authors.initials: S
authors.lastname: Benini
authors.forename: J J
authors.initials: JJ
authors.lastname: Van Beeumen
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003574:Cytochrome c Group / Q000737:chemistry* / Q000302:isolation & purification; D008074:Lipoproteins / Q000737:chemistry*; D013058:Mass Spectrometry; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D012995:Solubility
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008074:Lipoproteins; D010446:Peptide Fragments; C083951:cytochrome c553; C022302:cytochrome C-552; D008666:Metalloendopeptidases; C039830:peptidyl-Lys metalloendopeptidase; C058921:endoproteinase Asp-N