Peptide carriers: A helicoid-type sequential oligopeptide carrier (SOC(n)) for multiple anchoring of antigenic/immunogenic peptides.
A new peptide carrier with three-dimensional predetermined structural motif has been constructed by the repetitive Lys-Aib-Gly moiety. The sequential oligopeptide carrier (SOC(n)), (Lys-Aib-Gly)(n), adopts a distorted 3(10)-helical conformation and the Lys-N(epsilon)H(2) anchoring groups exhibit defined spatial orientations. Conformational analysis of the SOC(n) conjugates showed that the coupled peptides retain their initial "active" structure, while prevalence of one conformer was also observed. It is concluded that the beneficial structural elements of SOC(n) induce a favorable arrangement of the conjugated peptides, so that potent antigens and immunogens are generated.
Sakarellos-Daitsiotis
M
M
Section of Organic Chemistry and Biochemistry, University of Ioannina, Ioannina, G-45110, Greece.
Tsikaris
V
V
Vlachoyiannopoulos
P G
PG
Tzioufas
A G
AG
Moutsopoulos
H M
HM
Sakarellos
C
C
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D007114:Immunization; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011817:Rabbits
D000941:Antigens; D004337:Drug Carriers; D009842:Oligopeptides; D010455:Peptides