title: Determination of famotidine in human plasma and urine by high-performance liquid chromatography.
abstract: An improved, rapid and specific high-performance liquid chromatographic assay was developed for the determination of famotidine in human plasma and urine. Plasma samples were alkalinized and the analyte and internal standard (cimetidine) extracted with water-saturated ethyl acetate. The extracts were reconstituted in mobile phase, and injected onto a C18 reversed-phase column; UV detection was set at 267 nm. Urine samples were diluted with nine volumes of a mobile phase-internal standard mixture prior to injection. The lower limits of quantification in plasma and urine were 75 ng/ml and 1.0 microg/ml, respectively; intra- and inter-day coefficients of variation were < or =10.5%. This method is currently being used to support renal function studies assessing the use of intravenously administered famotidine to characterize cationic tubular secretion in man.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, PA 15261, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Dowling
authors.forename: R F
authors.initials: RF
authors.lastname: Frye
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015738:Famotidine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006634:Histamine H1 Antagonists / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D011786:Quality Control
chemical_list: D006634:Histamine H1 Antagonists; D015738:Famotidine