[Measurement of antiganglioside autoantibodies by immunodot-blot assay: clinical importance in peripheral neuropathies].
We retrospectively evaluated measurement data and clinical relevance of autoantibodies to gangliosides in peripheral neuropathies (PN). The IgG and IgM antiganglioside autoantibodies were determined by our own immunodot-blot assay on membrane and by enzyme-linked immunosorbent assay (Elisa) in sera of 1,342 patients with peripheral neuropathies. Anti-GM1 and anti-GD1b autoantibodies formed a part of the normal autoantibody repertoire and were common place in 12% of normal subjects and in 14% of disease control groups. Polyclonal IgM antiganglioside autoantibodies were detected in chronic PN, polyclonal IgG antiganglioside autoantibodies were detected in acute PN. Polyclonal IgM anti-GM1 and anti-GD1b autoantibodies were detected in 35 patients out of 48 with treatable multifocal motor neuropathy with persistent conduction blocks. These autoantibodies well discriminated between suspected motor peripheral neuropathies and motor neuron diseases (sensitivity 73%, specificity 83%, positive predictive value 60%, negative predictive value 91%). Monoclonal IgM autoantibodies reacted strongly with gangliosides in 15 patients out of 77 with M-IgM neuropathy (19%). M-IgM autoantibodies differed in their fine specificities with different principal target antigens as demonstrated with cross-reactivity. Such findings provide further evidence for a relationship between neurological syndromes and antiganglioside antibody profiles and also suggest that different gangliosides could be principal target antigens such as GM1, GD1b, GT1b, GD1a or GM2. Polyclonal IgG anti-GM1 and anti-GD1b autoantibodies were detected in 21 patients out of 22 with acute motor axonal Guillain-Barré syndrome with antecedent of infection by Campylobacter jejuni, polyclonal IgG anti-GQ1b autoantibodies in 9 patients out of 10 with Miller-Fisher syndrome. Detection of antiganglioside autoantibodies by immunodot-blot assay which is simple and quick in testing a large panel of gangliosides has become very important in the diagnosis and in the choose of expensive therapeutic strategies in chronic or acute autoimmune neuropathies.
Caudie
C
C
Laboratoire d'immunologie et de neuro-immunologie, Hôpital neurologique et neurochirurgical Pierre-Wertheimer, 59, boulevard Pinel, 69003 Lyon.
Vial
C
C
Petiot
P
P
Bancel
J
J
Later
R
R
Gonnaud
P M
PM
D000208:Acute Disease; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000175:diagnosis*; D002169:Campylobacter Infections / Q000276:immunology; D016123:Campylobacter jejuni; D002908:Chronic Disease; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000097:blood / Q000276:immunology; D005678:G(M2) Ganglioside / Q000097:blood / Q000276:immunology; D005732:Gangliosides / Q000097:blood / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D019846:Miller Fisher Syndrome / Q000276:immunology; D016472:Motor Neuron Disease / Q000276:immunology; D009414:Nerve Growth Factors / Q000276:immunology; D009431:Neural Conduction / Q000276:immunology; D010523:Peripheral Nervous System Diseases / Q000175:diagnosis / Q000276:immunology*; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D005732:Gangliosides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009414:Nerve Growth Factors; C021298:ganglioside, GD1a; C033851:ganglioside, GD1b; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1; C039129:GQ1b ganglioside