Effect of delivery system on the pharmacokinetic and phototherapeutic properties of bis(methyloxyethyleneoxy) silicon-phthalocyanine in tumor-bearing mice.
A Si(IV)-phthalocyanine bearing two methoxyethyleneglycol axial ligands bound to the central metal ion (SiPc) has been prepared by chemical synthesis and analyzed for its phototherapeutic activity after administration in a Cremophor or liposome formulation to C57B1/6 mice bearing a subcutaneously transplanted Lewis lung carcinoma (LLC). The maximum drug accumulation in the tumor is found at 24 h after intraperitoneal injection, independent of the delivery system. However, the tumor concentration of SiPc in the Cremophor formulation is about two-fold higher, while the drug concentration in liver and skin shows similar trends with the two delivery systems. The drug accumulation and retention in the brain is much larger when using Cremophor emulsion. Photodynamic therapy (672 nm, 370 mW m-2, 360 J cm-2) at 24 h after the injection of Cremophor emulsion- or DPPC liposome-formulated SiPc causes a very efficient and similar response for the LLC (approximately 8 versus 22 mm mean tumor diameter for the control groups at 21 days after phototreatment). These very promising effects, obtained both at higher and lower tumor drug concentrations, clearly demonstrate the potential phototherapeutical activity of the newly synthesized SiPc.
Wöhrle
D
D
Institute of Organic and Macromolecular Chemistry, University of Bremen, Germany.
Muller
S
S
Shopova
M
M
Mantareva
V
V
Spassova
G
G
Vietri
F
F
Ricchelli
F
F
Jori
G
G
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D001921:Brain / Q000378:metabolism; D004655:Emulsions; D005260:Female; D007211:Indoles / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D008081:Liposomes; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D017646:Organosilicon Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D014677:Pharmaceutical Vehicles; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011092:Polyethylene Glycols; D012867:Skin / Q000378:metabolism; D014018:Tissue Distribution
D004655:Emulsions; D007211:Indoles; D008081:Liposomes; D017646:Organosilicon Compounds; D014677:Pharmaceutical Vehicles; D017319:Photosensitizing Agents; C122090:bis(methyloxyethyleneoxy)silicon phthalocyanine; D015060:1,2-Dipalmitoylphosphatidylcholine; C022131:cremophor; D011092:Polyethylene Glycols