title: Suppression of serotonin hyperinnervation does not alter the dysregulatory influences of dopamine depletion on striatal neuropeptide gene expression in rodent neonates.
abstract: Sixty days following neonatal dopamine depletion (>98%) with 6-hydroxydopamine, preprotachykinin and preprodynorphin mRNA levels were significantly reduced (67 and 78% of vehicle controls, respectively) in the anterior striatum as determined by in situ hybridization while preproenkephalin mRNA expression was elevated (133% of vehicle controls). Suppression of the serotonin hyperinnervation phenomenon in the dopamine-depleted rat with 5,7-dihydroxytryptamine yielded no significant alterations in reduced striatal preprotachykinin (66%) or preprodynorphin (64%) mRNA levels, while preproenkephalin mRNA expression remained significantly elevated (140%). These data suggest that striatal serotonin hyperinnervation does not contribute to the development of dysregulated striatal neuropeptide transmission in either direct or indirect striatal output pathways following neonatal dopamine depletion.
authors.affiliation: Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Ml 48201, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Basura
authors.forename: P D
authors.initials: PD
authors.lastname: Walker
mesh_terms: D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003714:Denervation; D004298:Dopamine / Q000378:metabolism*; D004399:Dynorphins / Q000032:analysis; D004745:Enkephalins / Q000032:analysis; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016627:Oxidopamine / Q000494:pharmacology; D011498:Protein Precursors / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D015320:Tachykinins / Q000032:analysis
chemical_list: D004745:Enkephalins; D009479:Neuropeptides; D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C036448:pre-prodynorphin; C044206:preprotachykinin; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D004399:Dynorphins; D016627:Oxidopamine; C033660:preproenkephalin; D004298:Dopamine