Lectin histochemistry of the esophagus in several mammalian species.
The mucosa of the esophagus consists of stratified squamous epithelium that has a considerable resistance to injury. Intercellular glycoconjugates appear to constitute a major permeability barrier in the superficial portion of the esophageal mucosa. In the present study, we used a panel of lectins to investigate the differences in glycoconjugate production among different mammalian species. A battery of 12 lectins was used to study binding in sections from the esophagus of 6 mammalian species, including man. In general, the strongest staining was obtained in the stratum superficiale and the weakest staining in the stratum germinativum. In rabbit esophagus, exposure to pepsin/HCl produced a superficial damage to the epithelium, a considerable decrease in electrical resistance and a decreased staining of the esophageal epithelium with selected lectins. Pretreatment of the esophageal mucosa with sucrose octasulfate, a compound with protective properties, prevented, to some extent, the decrease in resistance and lectin staining.
Poorkhalkali
N
N
Preclinical Research and Development, Astra Hässle AB, S-43183 Mölndal, Sweden.
Jacobson
I
I
Helander
H F
HF
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D002415:Cats; D004285:Dogs; D004947:Esophagus / Q000737:chemistry* / Q000166:cytology* / Q000502:physiology; D005289:Ferrets; D005455:Fluorescent Antibody Technique; D006001:Glycoconjugates / Q000032:analysis* / Q000502:physiology*; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D037102:Lectins / Q000032:analysis*; D010434:Pepsin A / Q000494:pharmacology; D011817:Rabbits; D013395:Sucrose / Q000031:analogs & derivatives / Q000494:pharmacology; D013552:Swine
D000897:Anti-Ulcer Agents; D006001:Glycoconjugates; D037102:Lectins; D013395:Sucrose; D010434:Pepsin A; C053081:sucrose octasulfate; D006851:Hydrochloric Acid