Characterization of Mg(2+) efflux from rat erythrocytes non-loaded with Mg(2+).
Non-Mg(2+)-loaded rat erythrocytes with a physiological level of Mg(2+)(i) exhibited Mg(2+) efflux when incubated in nominally Mg(2+)-free media. Two types of Mg(2+) efflux were shown: (1) An Na(+)-dependent Mg(2+) efflux in NaCl and Na gluconate medium, which was inhibited by amiloride and quinidine, as was Na(2+)/Mg(2+) antiport in Mg(2+)-loaded rat erythrocytes; and (2) an Na(+)-independent Mg(2+) efflux in sucrose medium and choline Cl medium, which may be differentiated into SITS-sensitive Mg(2+) efflux at low Cl(-)(o) (in sucrose) and into SITS-insensitive Mg(2+) efflux at high Cl(-)(o) (in 150 mmol/l choline Cl).
Ebel
H
H
Institut für Klinische Physiologie, Klinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200, Berlin, Germany. ebel@ukbf.fu-berlin.de
Günther
T
T
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D017920:Antiporters / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002413:Cations, Divalent / Q000378:metabolism; D002478:Cells, Cultured; D002794:Choline; D004912:Erythrocytes / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D005942:Gluconates; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride; D013395:Sucrose
D017920:Antiporters; D002413:Cations, Divalent; D005942:Gluconates; C046250:sodium-magnesium antiporter; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D012965:Sodium Chloride; D013395:Sucrose; D003994:Bucladesine; D000584:Amiloride; D005665:Furosemide; D008274:Magnesium; D011802:Quinidine; D002794:Choline; C030691:gluconic acid