title: Intracellular distribution of hepatic glucokinase and glucokinase regulatory protein during the fasted to refed transition in rats.
abstract: We have studied the intracellular distribution in vivo of glucokinase (GK) and glucokinase regulatory protein (GKRP) in livers of fasted and refed rats, using specific antibodies against both proteins and laser confocal fluorescence microscopy. GK was found predominantly in the nucleus of hepatocytes from starved rats. GK was translocated to the cytoplasm in livers of 1- and 2-h refed animals, but returned to the nucleus after 4 h. GKRP concentrated in the hepatocyte nuclei and its distribution did not change upon refeeding. These results show that, in physiological conditions, GKRP is present predominantly in the nuclei of hepatocytes and that the translocation of hepatic GK from and to the nucleus is operative in vivo.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Martí i Franquès 1, Planta 7, E-08028, Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Novell
authors.forename: S
authors.initials: S
authors.lastname: Castel
authors.forename: D
authors.initials: D
authors.lastname: Bellido
authors.forename: J C
authors.initials: JC
authors.lastname: Ferrer
authors.forename: S
authors.initials: S
authors.lastname: Vilaró
authors.forename: J J
authors.initials: JJ
authors.lastname: Guinovart
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D005215:Fasting; D005455:Fluorescent Antibody Technique; D005502:Food; D005941:Glucokinase / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009752:Nutritional Status; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; C499484:Gckr protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C080906:glucokinase regulatory protein; D005941:Glucokinase