title: Four genes encoding different type I signal peptidases are organized in a cluster in Streptomyces lividans TK21.
abstract: Four adjacent genes (sipW, sipX, sipY and sipZ) encoding different type I signal peptidases, were isolated on a 7860 bp DNA fragment from Streptomyces lividans TK21. Three of the sip genes constitute an operon and the fourth is the first gene of another operon encompassing three additional, unrelated genes. A DNA fragment containing the four sip genes complemented an Escherichia coli type I signal peptidase mutant when cloned in a multicopy plasmid. Clustering of four different type I signal peptidase genes seems, so far, to be a unique feature of Streptomyces.
authors.affiliation: Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
authors.forename: Vı Ctor
authors.initials: VC
authors.lastname: Parro
authors.affiliation: Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium2.
authors.forename: Sabine
authors.initials: S
authors.lastname: Schacht
authors.affiliation: Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium2.
authors.forename: Jozef
authors.initials: J
authors.lastname: Anné
authors.affiliation: Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
authors.forename: Rafael P
authors.initials: RP
authors.lastname: Mellado
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012697:Serine Endopeptidases; C026515:type I signal peptidase