Computer-aided development of a high-performance liquid chromatographic method for the determination of hydroxyanthraquinone derivatives in Chinese herb medicine rhubarb.
With computer simulation predicting separation in reversed-phase gradient elution, a method to separate and determine five hydroxyanthraquinone derivatives having a wide range of polarity in extract of Chinese herbal medicine rhubarb has been developed. The software DryLab was used to model the retention behavior of the compounds as a function of gradient conditions, based on data from two scouting gradient runs. Under the optimized conditions, i.e. column, Zorbax RX-C18, 5 microm, 15x0.46 cm; solvent A, 36 mM triethylamine phosphate (TEAP), pH=2.5; solvent B, ACN; gradient, 36/36/80/80% B at 0/5.5/20.5/25.5 min; flow-rate, 1.00 ml/min; temperature, ambient, the method was successfully applied to monitor the quality of rhubarb from different sources. The effect of sample preparation procedures on extraction efficiency was also examined.
Liu
C L
CL
Department of Chemistry, Lanzhou University, China.
Zhu
P L
PL
Liu
M C
MC
D000880:Anthraquinones / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003201:Computers; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal; D012250:Rheum / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal