Analysis and regulation of the secY gene(1) from Streptomyces griseus N2-3-11 and interaction of the SecY protein with the SecA protein.
The chromosomal region encoding the secY gene of Streptomyces griseus N2-3-11 was cloned and analyzed. The secY gene encodes a polypeptide of 438 aa with a molecular mass of 47.5 kDa. The transcriptional start point of the secY gene was determined. Northern blot analysis revealed a growth phase-dependent secY expression supporting our previous findings for secA gene expression in S. griseus. Overproduction of the SecY protein was obtained when using Streptomyces lividans TK23 as host. The interaction of the SecY proteins of S. griseus, S. lividans, and Escherichia coli, respectively, with the purified SecA protein of S. griseus was demonstrated for the first time by using ligand affinity blot assays.
Pöhling
S
S
Bergische Universität GH Wuppertal, Chemische Mikrobiologie, D-42097, Wuppertal, Germany.
Piepersberg
W
W
Wehmeier
U F
UF
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D013305:Streptomyces griseus / Q000235:genetics*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins