Combined enhancement of microtubule assembly and glucose metabolism in neuronal systems in vitro: decreased sensitivity to copper toxicity.
Brain cell-free extract greatly stimulates the polymerization rate of purified tubulin with a reduction of the nucleation period and without a significant alteration of the final assembly state. This effect is mimicked by neuroblastoma extract at 10-fold lower extract concentration, but not by excess muscle extract. Copper inhibits microtubule assembly in vitro but in the presence of brain extract the copper effect is suspended. Electron microscopic images showed that intact microtubules are formed and decorated by cytosolic proteins in the absence and presence of copper, while the copper alone induces the formation of S-shaped sheets and oligomeric threads. The flux of triosephosphate formation from glucose is enhanced by microtubules in brain extract, but not in muscle extract. Copper inhibits the glycolytic flux; however, the presence of microtubules not only suspends the inhibition by copper but the activation of glycolysis by microtubules is also preserved. We conclude that the organization of neuronal proteins modifies both the rates of microtubule assembly and glycolysis, and reduces their sensitivities against the inhibition caused by copper.
Liliom
K
K
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, H-1518, Hungary.
Wágner
G
G
Kovács
J
J
Comin
B
B
Cascante
M
M
Orosz
F
F
Ovádi
J
J
D000818:Animals; D001921:Brain / Q000187:drug effects; D002417:Cattle; D003300:Copper / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D008868:Microtubule Proteins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D014020:Tissue Extracts / Q000494:pharmacology; D014404:Tubulin / Q000737:chemistry
D008868:Microtubule Proteins; D014020:Tissue Extracts; D014404:Tubulin; D003300:Copper; D005947:Glucose; C029892:cupric chloride