title: Isolation and identification by sequence homology of a second putative C5-DNA-methyltransferase gene from Ascobolus immersus.
abstract: I report the cloning of a new Ascobolus gene (masc 2) that potentially encodes a C5-DNA-methyltransferase. The putative protein exhibits the two domains characteristic of eukaryotic maintenance DNA-methyltransferase: a large N-terminal domain and a C-terminal domain containing all ten catalytic motifs arranged in the canonical order. A new type of eukaryotic DNA-methylase gene (masc 1) has been recently found in Ascobolus. Masc1 is essential for the de novo methylation and dispensable for methylation maintenance. The masc2 gene could encode the methylase involved in this maintenance.
authors.affiliation: Institut de Génétique et Microbiologie, CNRS/Université Paris-Sud, Orsay, France.
authors.forename: C
authors.initials: C
authors.lastname: Goyon
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; C110879:Masc2 protein, Ascobolus immersus; D004248:DNA (Cytosine-5-)-Methyltransferases