Effects of leptin on spinally projecting neurons in the PVN of the hypothalamus.
The study examined whether intravenous (i.v.) leptin increased Fos-production in spinally projecting neurons in the hypothalamic paraventricular nucleus (PVN). We combined (i) rhodamine tagged microspheres injected into the upper thoracic spinal cord and (ii) Fos (marker of neuronal activation) immunohistochemistry. Effects of recombinant murine leptin were compared to vehicle (containing lipopolysaccharide a contaminant present in the leptin solution). Following leptin, 10% of the spinally projecting neurons contained Fos. However, vehicle elicited similar effects and there was no significant difference between the groups.
Badoer
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Department of Medicine, Monash Medical Centre, Monash University, 246 Clayton Rd., Clayton 3168, Melbourne, Australia. emilio9.badoer@med.monash.edu.au
Ryan
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D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D007275:Injections, Intravenous; D020738:Leptin / Q000494:pharmacology*; D009434:Neural Pathways; D009474:Neurons / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D013116:Spinal Cord / Q000166:cytology*
D020738:Leptin; D016760:Proto-Oncogene Proteins c-fos