Isolation of Rickettsia prowazekii from blood by shell vial cell culture.
A blood sample from a patient who returned from Algeria with a fever inoculated on human embryonic lung fibroblasts by the shell vial cell culture technique led to the recovery of Rickettsia prowazekii. The last clinical strain was isolated 30 years ago. Shell vial cell culture is a versatile method that could replace the classic animal and/or embryonated egg inoculation.
Birg
M L
ML
Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 13385 Marseille Cedex 05, France.
La Scola
B
B
Roux
V
V
Brouqui
P
P
Raoult
D
D
D000368:Aged; D000462:Algeria; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002642:Chick Embryo; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012283:Rickettsia prowazekii / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014195:Travel; D014438:Typhus, Epidemic Louse-Borne / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial