title: A multistate nosocomial outbreak of Ralstonia pickettii colonization associated with an intrinsically contaminated respiratory care solution.
abstract: From 1 February through 30 April 1998, 4 hospitals reported a total of 34 patients colonized with Ralstonia pickettii. All but 1 had been exposed to 0.9% saline solution manufactured by 1 company (Modudose; Kendall, Mainsfield, MA), which was used during endotracheal suctioning. Culture of saline solution from previously unopened vials yielded R. pickettii. All available product and patient isolates were genotypically related by pulsed-field gel electrophoresis (PFGE) analysis. The contaminated saline solution was manufactured at the same plant that had been associated with a similar outbreak in 1983. The 1983 and 1998 R. pickettii isolates were unrelated, as determined by PFGE. In both 1983 and 1998, a 0. 2-microm cartridge filter was used for terminal sterilization. The detection of R. pickettii should alert hospital personnel to the possibility of product contamination. In this outbreak, prompt notification of public health agencies resulted in rapid notification of other health care providers, which likely prevented additional outbreaks.
authors.affiliation: Clinical Microbiology Laboratory, Department of Pathology and Laboratory Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Labarca
authors.forename: W E
authors.initials: WE
authors.lastname: Trick
authors.forename: C L
authors.initials: CL
authors.lastname: Peterson
authors.forename: L A
authors.initials: LA
authors.lastname: Carson
authors.forename: S C
authors.initials: SC
authors.lastname: Holt
authors.forename: M J
authors.initials: MJ
authors.lastname: Arduino
authors.forename: M
authors.initials: M
authors.lastname: Meylan
authors.forename: L
authors.initials: L
authors.lastname: Mascola
authors.forename: W R
authors.initials: WR
authors.lastname: Jarvis
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003428:Cross Infection / Q000209:etiology*; D004196:Disease Outbreaks; D004340:Drug Contamination; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D016946:Gram-Negative Aerobic Rods and Cocci / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride