title: Crystallization and preliminary x-ray structure determination of Lupinus luteus PR10 protein.
abstract: The pathogenesis-related protein of the PR10 class from Lupinus luteus (yellow lupin), LlPR10.1A, is constitutively expressed in roots. It is also accumulated in leaves treated with a suspension of pathogenic bacteria as a response to stress. Recombinant yellow-lupin LlPR10.1A protein has been overexpressed in Escherichia coli as a fusion product with maltose-binding protein. LlPR10.1A crystallizes in the orthorhombic P2(1)2(1)2(1) space group and the crystals diffract to 2.45 A resolution. The structure has been solved by molecular replacement, using the structure of a birch-pollen allergen protein as a model.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61--704 Poznań, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Biesiadka
authors.forename: M M
authors.initials: MM
authors.lastname: Sikorski
authors.forename: G
authors.initials: G
authors.lastname: Bujacz
authors.forename: M
authors.initials: M
authors.lastname: Jaskólski
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000485:Allergens; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D007887:Fabaceae; D058619:Maltose-Binding Proteins; D008958:Models, Molecular; D009004:Monosaccharide Transport Proteins; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D010946:Plants, Medicinal; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D000485:Allergens; D052179:Antigens, Plant; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C461599:maltose transport system, E coli; C053376:pathogenesis-related proteins, plant; C061312:Bet v 1 allergen, Betula