Regulation of high-affinity glutamate transport by amino acid deprivation and hyperosmotic stress.
High-affinity glutamate transport activity is induced by stress in NBL-1 cells. Exposure of cells to hyperosmotic medium led to an induction of the EAAC1 glutamate transporter, preceded by a large increase in EAAC1 mRNA levels. Culture of cells in amino acid-free medium also caused a protein synthesis-dependent increase in glutamate transport activity, but this was not accompanied by an increase of either EAAC1 mRNA or protein. Indirect evidence suggests that the increase in EAAC1 activity in the latter case may be due to the synthesis of an activator protein in response to decreased intracellular glutamate concentrations.
McGivan
J D
JD
Department of Biochemistry, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom. j.mcgivan@bristol.ac.uk
Nicholson
B
B
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG; D000596:Amino Acids / Q000172:deficiency*; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009994:Osmolar Concentration; D013312:Stress, Physiological / Q000139:chemically induced / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D000596:Amino Acids; D003470:Culture Media