SOS induction of selected naturally occurring substances in Escherichia coli (SOS chromotest).
Naturally occurring substances were tested for genotoxicity using a modified laboratory protocol of the Escherichia coli PQ37 genotoxicity assay (SOS chromotest) in the presence and in the absence of an exogenous metabolizing system from rat liver S9-mix. Aristolochic acid I, II, the plant extract aristolochic acid and psoralene were genotoxic; cycasine, emodine, monocrotaline and retrorsine were classified as marginal genotoxic in the SOS chromotest in the absence of S9-mix. In the presence of an exogenous metabolizing system from rat liver S9-mix aristolochic acid I, the plant extract, beta-asarone, cycasin, monocrotaline, psoralen and retrorsine showed genotoxic effects; aristolochic acid II marginal genotoxic effects. Arecoline, benzyl acetate, coumarin, isatidine dihydrate, reserpine, safrole, sanguinarine chloride, senecionine, senkirkine, tannin and thiourea revealed no genotoxicity in the SOS chromotest either in the presence or in the absence of an exogenous metabolizing system from rat liver S9-mix. For 17 of 20 compounds, the results obtained in the SOS chromotest could be compared to those obtained in the Ames test. It was found that 12 (70.6%) of these compounds give similar responses in both tests (6 positive and 6 negative responses). The present investigation and those reported earlier, the SOS chromotest, using E. coli PQ37, was able to detect correctly most of the Salmonella mutagens and non-mutagens.
Kevekordes
S
S
Medical Institute of General Hygiene and Environmental Health, University of Göttingen, Windausweg 2, 37073, Göttingen, Germany. skeveko@gwdg.de
Mersch-Sundermann
V
V
Burghaus
C M
CM
Spielberger
J
J
Schmeiser
H H
HH
Arlt
V M
VM
Dunkelberg
H
H
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D001426:Bacterial Proteins / Q000187:drug effects / Q000378:metabolism; D002457:Cell Extracts / Q000494:pharmacology; D004249:DNA Damage; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000737:chemistry; D009152:Mutagenicity Tests; D009930:Organic Chemicals / Q000633:toxicity*; D013014:SOS Response, Genetics / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D001426:Bacterial Proteins; D002457:Cell Extracts; D009930:Organic Chemicals; D000469:Alkaline Phosphatase