Effects of interleukin-6 (IL-6) on cytotrophoblastic cells.
Tumour invasion and trophoblastic invasion share the same biochemical mediators: the matrix metalloproteinases (MMP) and their inhibitors. In contrast to tumour invasion of a host tissue, trophoblastic invasion during implantation and placentation is stringently controlled both in tissue localization and developmental stage. The factors responsible for these important regulatory processes are unknown, but in-vitro studies point to endometrial cytokines and growth factors as possible candidates. Here we examined the possibility that interleukin-6 (IL-6), a trophoblastic and endometrial cytokine, represents such a regulatory factor. Purified first trimester cytotrophoblastic cells (CTB) were cultured for 4 days in presence or absence of increasing concentrations of IL-6. MMP-2 and MMP-9 bioactivity (zymography) and immunoactivity were measured in the culture supernatants together with total human chorionic gonadotrophin (HCG), fetal fibronectin (FFN) and leptin. IL-6 did not change the cytotrophoblastic secretion of FFN or total HCG. In contrast, this cytokine induced a dose-dependent stimulation of the leptin secretion and increased the activity, but not the immunoreactivity, of MMP-9 and MMP-2. These results indicate that IL-6 could be considered as an endometrio-trophoblastic regulator of cytotrophoblastic gelatinases.
Meisser
A
A
Department of Obstetrics and Gynaecology, Geneva University Hospital and WHO Collaborating Centre in Human Reproduction, University of Geneva, Switzerland.
Cameo
P
P
Islami
D
D
Campana
A
A
Bischof
P
P
D000028:Abortion, Induced; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000494:pharmacology*; D020738:Leptin / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D011247:Pregnancy / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
D007375:Interleukin-1; D015850:Interleukin-6; D020738:Leptin; D020780:Matrix Metalloproteinase 9