Structure and expression of the mouse homologue of the XK gene.
The human Kx blood group antigen is carried by a 37,000 M(r) apparent molecular mass membrane polypeptide which is deficient in rare individuals with the McLeod syndrome. The X-linked human XK gene is transcribed in many tissues including adult skeletal muscle and brain, sieges of disorders observed in McLeod syndrome. We report here the cloning of the orthologous mouse XK mRNA. Comparison of XK from human and mouse revealed 80% sequence similarity at the amino acid level. The mouse XK gene is organized in two exons and is expressed in many tissues, but its expression pattern is slightly different from that of the human gene. The presence in mouse erythrocyte membrane of a 43,000 M(r) Kx-related protein was demonstrated by immunoblotting with a rabbit antiserum directed against the human protein. With non-reduced samples, a 140,000 M(r) species was detected instead of the 43,000 M(r) protein, suggesting that, as demonstrated in the Kx polypeptide might be complexed with another protein in mouse red cells, presumably the homologue of the human Kell protein of 93,000 M(r).
Collec
E
E
INSERM U76, Institut National de la Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France.
Colin
Y
Y
Carbonnet
F
F
Hattab
C
C
Bertrand
O
O
Cartron
J P
JP
Kim
C L
CL
D000595:Amino Acid Sequence; D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D001789:Blood Group Antigens / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry; D015723:Gene Library; D007626:Kell Blood-Group System; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal; D011485:Protein Binding; D014018:Tissue Distribution
D026921:Amino Acid Transport Systems, Neutral; D001789:Blood Group Antigens; D002352:Carrier Proteins; D007626:Kell Blood-Group System; D008565:Membrane Proteins; C498286:Xkh protein, mouse