title: Unilateral blunt testicular trauma in pre-pubertal rats.
abstract: Injury to the testis breaching the tunica albuginea is known to affect fertility. Blunt testicular trauma with an intact tunica albuginea has been reported to have no effect on contralateral testicular histology and Johnsen testicular maturation score. However, sensitive techniques like DNA flowcytometry have not been utilized so far to evaluate contralateral testicular germ-cell changes. Sixty-four male Wistar rats aged 20 days were randomized into groups I (control), II (unilateral blunt testicular trauma, UBTT), III (UBTT and excision of ipsilateral testis at 6 h), and IV (UBTT and cyclosporine for 30 days). Fertility, DNA flow cytometry of contralateral testicular tissue, and anti-sperm antibodies (ASA) were evaluated. Fertility and haploid-cell percentage of the contralateral testis were significantly decreased compared to controls in early adulthood (100 days). Around 150 days of age, as ASA decreased significantly, fertility and contralateral testicular haploid-cell population recovered and were comparable to the controls. Excision of the traumatized testicle around 6 h after injury or administration of cyclosporine for 30 days protected fertility and the contralateral testis. In contrast to group II rats, ELISA for ASA was negative in group III and IV rats. UBTT affects the contralateral testis and fertility. ASA mediate this damage. Orchidectomy performed around 6 h after trauma or short-term cyclosporine therapy prevents the damage.
authors.affiliation: Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Srinivas
authors.forename: S
authors.initials: S
authors.lastname: Hashim
authors.forename: D K
authors.initials: DK
authors.lastname: Mitra
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D004247:DNA / Q000032:analysis; D005069:Evaluation Studies as Topic; D005298:Fertility; D005434:Flow Cytometry; D006238:Haploidy; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013094:Spermatozoa / Q000276:immunology; D013737:Testis / Q000166:cytology* / Q000293:injuries*; D014949:Wounds, Nonpenetrating
chemical_list: D000906:Antibodies; D004247:DNA