title: CRM1 mediates nuclear export of nonstructural protein 2 from parvovirus minute virus of mice.
abstract: The nonstructural protein 2 (NS2) from parvovirus minute virus of mice (MVMp) is a 25-kDa polypeptide which localizes preferentially to the cytoplasm and associates with cellular proteins in cytoplasm. These lines of evidence suggest that NS2 is positively exported from the nucleus to cytoplasm and functions in cytoplasm. We report here that nuclear export of NS2 is inhibited by leptomycin B (LMB), a drug that specifically blocks nuclear export signal (NES)-chromosomal region maintenance 1 (CRM1) interactions. CRM1 binds specifically to the 81- to 106-amino-acid (aa) region of NS2, and the region of NS2 actually functions as a NES. Interestingly, this region appears to be distinct from a typical NES sequence, which consists of leucine-rich sequences. These results indicate that NS2 protein is continuously exported from the nucleus by a CRM1-dependent mechanism and suggest that CRM1 also exports to distinct type of NESs.
authors.affiliation: Institute of Basic Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohshima
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Oishi
authors.forename: N
authors.initials: N
authors.lastname: Imamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Yoneda
authors.forename: A
authors.initials: A
authors.lastname: Fukamizu
authors.forename: K i
authors.initials: Ki
authors.lastname: Yagami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism* / Q000821:virology; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D028884:Karyopherins; D051379:Mice; D008915:Minute Virus of Mice / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D018160:Receptors, Cytoplasmic and Nuclear; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D000097606:Exportin 1 Protein
chemical_list: D002352:Carrier Proteins; D005231:Fatty Acids, Unsaturated; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D017361:Viral Nonstructural Proteins; D000097606:Exportin 1 Protein; C038753:leptomycin B