title: Autolysis of Escherichia coli and Bacillus subtilis cells in low gravity.
abstract: The role of gravity in the autolysis of Bacillus subtilis and Escherichia coli was studied by growing cells on Earth and in microgravity on Space Station Mir. Autolysis analysis was completed by examining the death phase or exponential decay of cells for approximately 4 months following the stationary phase. Consistent with published findings, the stationary-phase cell population was 170% and 90% higher in flight B. subtilis and E. coli cultures, respectively, than in ground cultures. Although both flight autolysis curves began at higher cell densities than control curves, the rate of autolysis in flight cultures was identical to that of their respective ground control rates.
authors.affiliation: University of Colorado, Engineering Center, Boulder, CO 80309-0429, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Kacena
authors.forename: E E
authors.initials: EE
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Todd
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology*; D001433:Bacteriolysis; D002473:Cell Wall; D004926:Escherichia coli / Q000166:cytology*; D013026:Space Flight; D013045:Species Specificity; D014893:Weightlessness