A unique bFGF-responsive transcriptional element.
The mitogen-regulated protein/proliferin (mrp/plf) genes encode closely related proteins that stimulate cell proliferation and angiogenesis. Basic fibroblast growth factor (bFGF) increases mrp/plf mRNA and protein production by 3T3 cells. Although the three cloned mrp/plf gene promoters are over 97% identical, only mrp3 is transcriptionally activated by bFGF. A series of truncated mrp3 promoter sequences were tested to determine the minimal promoter sequence necessary for bFGF-responsive transcription. Within the minimal bFGF-responsive mrp3 promoter fragment, a putative FGF-regulatory element (FRE) was identified. Nuclear factors that bind the FRE are present in 3T3 cells. When present upstream of a thymidine kinase basal promoter, the FRE exhibits high transcriptional activity and responds to bFGF. Thus, the FRE is a strong transcriptional element that is regulated by bFGF and that may participate in regulating the mrp3 gene and perhaps other FGF-regulated genes.
Mohideen
M A
MA
Department of Biochemistry and Biophysics and the Molecular, Cellular, Iowa State University, Ames 50011, USA.
Hruska-Hageman
A
A
Nilsen-Hamilton
M
M
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D001483:Base Sequence; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011388:Prolactin; D011401:Promoter Regions, Genetic; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex; D012045:Regulatory Sequences, Nucleic Acid; D020218:Response Elements / Q000187:drug effects / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D005656:Fungal Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011768:Pyruvate Dehydrogenase Complex; D012269:Ribosomal Proteins; D016222:Fibroblast Growth Factor 2; C067358:MRP-3 protein, Neurospora crassa; D011388:Prolactin; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D013937:Thymidine Kinase; D018093:Gelatinases; D019278:Matrix Metalloproteinase 3; D013755:Tetradecanoylphorbol Acetate