title: Type 2 rice metallothionein-like gene has two introns.
abstract: A type 2 rice metallothionein-like gene was isolated from root by PCR and sequenced. The PCR fragment was designated as pcr1460, which overlaps with OsMT-2, a cDNA sequence previously characterized, with the presence of two additional segments, 583 and 613 bp in length. These segments are recognized as introns which divide the coding sequence into three exons, 65, 78 and 106 base pairs in length. The sequences flanking the introns conform with the GT/AG rule for splice junctions, and one exonic open reading frame can be identified in each of the introns. The observation that MT-like gene has two introns is the first of such a finding obtained from monocotyledonous plants.
authors.affiliation: Department of Life Science, National Tsing Hua University, Taiwan, ROC.
authors.forename: W M
authors.initials: WM
authors.lastname: Chen
authors.forename: H M
authors.initials: HM
authors.lastname: Hsieh
authors.forename: P C
authors.initials: PC
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D017343:Genes, Plant; D006801:Humans; D007438:Introns / Q000235:genetics*; D008668:Metallothionein / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: C103078:MT-2 protein, Oryza sativa; D010940:Plant Proteins; D008668:Metallothionein