title: Empirical midpoint dissociation temperature (Td) determination for oligonucleotide probes using a PCR thermal cycler.
authors.affiliation: Harvard University, Cambridge, MA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Gulledge
authors.forename: C M
authors.initials: CM
authors.lastname: Cavanaugh
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013696:Temperature; D013816:Thermodynamics
chemical_list: D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S