title: The length of the polyoxyethylene chain in the Triton X detergents modulates the apparent activation of neurosteroid sulfatase in bovine brain.
abstract: The effect of the Triton X series on the solubilization and enzyme activity of neurosteroid sulfatase (NSS) in the bovine midbrain was investigated. Triton X-100 and X165 stimulated NSS activity in the bovine midbrain, while Triton X-305 did not. This apparent activation was attributed to the action of the detergents, and not to the latency of the enzyme or the removal of some inhibitory substance from the microsomes. The maximum stimulation was obtained when the length of the polyoxyethylene chain of the detergent was 16.
authors.affiliation: Department of Biotechnology, College of Engineering, Ajou University, Suwon, South Korea.
authors.forename: I H
authors.initials: IH
authors.lastname: Park
authors.forename: B K
authors.initials: BK
authors.lastname: Han
authors.forename: S J
authors.initials: SJ
authors.lastname: Cho
authors.forename: D H
authors.initials: DH
authors.lastname: Jo
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D003902:Detergents / Q000737:chemistry*; D004789:Enzyme Activation; D051379:Mice; D008861:Microsomes / Q000201:enzymology; D008956:Models, Chemical; D017830:Octoxynol / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012995:Solubility; D013429:Sulfatases / Q000378:metabolism*
chemical_list: D003902:Detergents; D011092:Polyethylene Glycols; D017830:Octoxynol; D013429:Sulfatases