title: Synthesis of polyacrylamides N-substituted with PNA-like oligonucleotide mimics for molecular diagnostic applications.
abstract: Two types of oligonucleotide mimics relative to peptide nucleic acids (PNAs) were tested as probes in nucleic acid hybridisation assays based on polyacrylamide technology. One type of mimic oligomers represented a chimera constructed of PNA and phosphono-PNA (pPNA) monomers, and the other one contained pPNA residues alternating with PNA-like monomers on the base of trans -4-hydroxy-L-proline (HypNA). A chemistry providing efficient and specific covalent attachment of these DNA mimics to acrylamide polymers using a convenient approach based on the co-polymerisation of acrylamide and some reactive acrylic acid derivatives with oligomers bearing 5'- or 3'-terminal acrylamide groups has been developed. A comparative study of polyacrylamide conjugates with oligonucleotides and mimic oligomers demonstrated the suitability and high potential of PNA-pPNA and HypNA-pPNA chimeras as sequence-specific probes in capture and detection of target nucleic acid fragments to serve current forms of DNA arrays.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya 16/10, Moscow 117871, Russia. eva@ibch.siobc.ras.ru
authors.forename: V A
authors.initials: VA
authors.lastname: Efimov
authors.forename: A A
authors.initials: AA
authors.lastname: Buryakova
authors.forename: O G
authors.initials: OG
authors.lastname: Chakhmakhcheva
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D019937:Diagnostic Techniques and Procedures; D018716:Molecular Mimicry; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D020106:Acrylamide; D000180:Acrylic Resins; D004247:DNA; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D000099166:Polyacrylamides