title: The possible role of isoforms of cytochrome c oxidase subunit VIa in mammalian thermogenesis.
abstract: A single cDNA of cytochrome c oxidase subunit VIa was characterised from liver, heart and the thermogenic organ of the partially endotherm tuna fish. The amino acid sequence revealed high identity with subunit VIa from carp and trout, but low identity to subunits VIaL (liver type) and VIaH (heart type) of mammalian cytochrome c oxidase. In reconstituted cytochrome c oxidase from bovine heart, the H+/e- stoichiometry is decreased from 1.0 to 0.5 at high intraliposomal ATP/ADP ratios via exchange of bound ADP by ATP at the matrix domain of the transmembraneous subunit VIaH. Reconstituted cytochrome c oxidase from bovine liver and kidney, containing subunit VIaL, revealed H+/e- ratios below 0.5, independent of the ATP/ADP ratio. The results suggest the evolution of three types of subunit VIa. Subunits VIaH and VIaL are postulated to participate in mammalian thermogenesis.
authors.affiliation: Fachbereich Chemie, Philipps-Universität, Marburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hüttemann
authors.forename: V
authors.initials: V
authors.lastname: Frank
authors.forename: B
authors.initials: B
authors.lastname: Kadenbach
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001833:Body Temperature Regulation / Q000502:physiology*; D002347:Carps; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D008099:Liver / Q000201:enzymology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010802:Phylogeny; D011487:Protein Conformation; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014337:Trout; D014413:Tuna
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D003576:Electron Transport Complex IV