Anoxia/induced membrane changes in human red blood cells.
The cation-osmotic hemolysis was studied in human red blood cells incubated under anoxic conditions. In relation to the time course of anoxia, two phases of hemolysis were distinguished. A significant decrease of hemolysis was found between 3 and 24 h of incubation. On the other hand, hemolysis was significantly increased after prolonged incubation (48-72 h). Using the method of cation-osmotic hemolysis, the properties of two membrane constituents, spectrine membrane skeleton and membrane bilayer, were studied. The relation between cation-osmotic hemolysis and erythrocyte deformability is being discussed.
Nicák
A
A
Department of Pharmacology, Medical Faculty, Safárik University, Slovak Republic. mojzis@central.medic.upjs.sk
Mojzis
J
J
Jandoseková
M
M
Mirossay
L
L
D004910:Erythrocyte Membrane / Q000473:pathology*; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology; D006461:Hemolysis; D006801:Humans; D000860:Hypoxia / Q000097:blood / Q000473:pathology*