Structural elucidation of the O-antigenic polysaccharides from Escherichia coli O21 and the enteroaggregative Escherichia coli strain 105.
The structure of the O-antigen polysaccharide of the lipopolysaccharide from an enteroaggregative Escherichia coli (strain 105) has been elucidated, using primarily one-dimensional and two-dimensional NMR experiments. The sequence of residues was deduced with heteronuclear multiple-bond correlation and NOESY experiments. The structure of the repeating unit of the polysaccharide from the enteroaggregative E. coli is as follows:[sequence: see text] The structure of the O-antigen from enteroaggregative E. coli strain 105 was shown to be identical with that of E. coli O21 by sugar and methylation analyses as well as by 1H-NMR and 13C-NMR spectroscopy.
Staaf
M
M
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Urbina
F
F
Weintraub
A
A
Widmalm
G
G
D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D002449:Cell Aggregation; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D004196:Disease Outbreaks; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D014774:Virulence
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial