Arginyl-tRNA-protein transferase activities in crude supernatants of rat tissues.
A fluorescent HPLC method for the assay of arginyl-tRNA-protein transferase (R-Transferase) activity was applied to obtain quantitative data of the enzyme activity in rat tissues for the first time. In this assay, the major problem was a significant hydrolysis of the substrate, N-aspartyl-N'-dansylamido-1,4-butanediamine, and the product, N-arginylaspartyl-N'-dansylamido-1,4-butanediamine (ArgAsp(4)DNS) by aminopeptidases in crude samples such as 105000g supernatants (105S) of tissue homogenates. As bestatin inhibited the hydrolysis of ArgAsp(4)DNS, a standard-addition method in the presence of bestatin, using a partially purified R-Transferase preparation from hog kidney as a standard, made it possible to measure directly R-Transferase activities in 105S with a short incubation time and sufficient reliability. It was found by the established method that of 14 tissues examined, stomach was rich in the R-Transferase activity with the highest specific activity, suggesting a target tissue for the future studies on R-Transferase to elucidate its physiological significance.
Takao
K
K
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
Samejima
K
K
D019881:Aminoacyltransferases / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007668:Kidney / Q000201:enzymology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods; D013552:Swine; D014018:Tissue Distribution; D014020:Tissue Extracts / Q000378:metabolism
D014020:Tissue Extracts; D019881:Aminoacyltransferases; C019727:arginyltransferase