title: Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy.
abstract: BACKGROUND
  Na(+)/H(+) exchangers (NHE) are transporters involved in the absorption of NaCl along the gastrointestinal tract. The aim of this study was to determine the expression pattern of the intestinal brush border NHE isoforms 2 and 3 following massive small bowel resection (SBR). Additionally, the effect of epidermal growth factor (EGF) and salivarectomy (removal of the primary source of EGF) on the expression pattern was studied.
  
  
  METHODS
  ICR mice underwent a proximal SBR or sham surgery and then received either orogastric saline or EGF (50 microg/kg/day). In separate experiments mice underwent salivarectomy followed by SBR or sham. Postoperatively the remaining ileum was isolated and levels of NHE-2 and NHE-3 mRNA and protein were resolved.
  
  
  RESULTS
  Following SBR, the expression of both mRNA and protein for NHE-3 increased by approximately 2.5-fold. Treatment with EGF enhanced NHE-3 mRNA in sham animals with further elevation following SBR. The expression of NHE-2 mRNA demonstrated minimal change while protein marginally increased (40%) following SBR. EGF did not affect the expression of NHE-2 mRNA. Salivarectomy did not influence NHE-2 protein expression and inhibited the increased NHE-3 protein expression following SBR.
  
  
  CONCLUSIONS
  Following SBR, the expression pattern for brush border NHE isoforms is distinctive. Increased expression of NHE-3 secondary to SBR and/or EGF treatment with loss of this increase following salivarectomy implies a common mechanism to enhance enterocyte proliferation and luminal absorption of NaCl and water. These results suggest that NHE-3 is an important ileal exchanger following SBR.
authors.affiliation: Department of Surgery, Children's Hospital Medical Center, Cincinnati, Ohio, 45229, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Falcone
authors.forename: C E
authors.initials: CE
authors.lastname: Shin
authors.forename: L E
authors.initials: LE
authors.lastname: Stern
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: C R
authors.initials: CR
authors.lastname: Erwin
authors.forename: M
authors.initials: M
authors.lastname: Soleimani
authors.forename: B W
authors.initials: BW
authors.lastname: Warner
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects / Q000378:metabolism* / Q000601:surgery*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011184:Postoperative Period; D012333:RNA, Messenger / Q000378:metabolism; D012469:Salivary Glands / Q000502:physiology / Q000601:surgery; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C513111:Slc9a2 protein, mouse; C000613386:Slc9a3 protein, mouse; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D004815:Epidermal Growth Factor