The dnaA gene region of Mycobacterium avium and the autonomous replication activities of its 5' and 3' flanking regions.
A 3.9 kb DNA fragment containing the dnaA gene region of Mycobacterium avium was cloned and its nucleotide sequence was determined. Nucleotide sequence analyses indicated that this region encodes three genes in the order rpmH (ribosomal protein L34), dnaA (the putative initiator protein) and dnaN (the beta subunit of DNA polymerase III). The intergenic regions between the rpmH-dnaA and dnaA-dnaN genes were found to contain several putative DnaA boxes, 9 nt long DnaA protein recognition sequences. A DNA fragment containing the 3' but not the 5' flanking region of the M. avium dnaA gene when cloned in Escherichia coli plasmids, which are otherwise non-replicative in mycobacteria, exhibited autonomous replication activity in M. avium but not in Mycobacterium bovis BCG and Mycobacterium smegmatis. The 5' flanking region of dnaA, on the other hand, exhibited autonomous replication activity in M. bovis BCG but not in M. avium and M. smegmatis. The implications of these results for the understanding of the M. avium oriC replication initiation process are discussed.
Madiraju
M V
MV
Department of Biochemistry, The University of Texas Health Center at Tyler, 75708-3154, USA. murty@uthct.edu
Qin
M H
MH
Yamamoto
K
K
Atkinson
M A
MA
Rajagopalan
M
M
D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D008969:Molecular Sequence Data; D015269:Mycobacterium avium Complex / Q000235:genetics*; D009163:Mycobacterium bovis / Q000235:genetics; D020102:Mycobacterium smegmatis / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D018741:Replication Origin / Q000235:genetics / Q000502:physiology; D012269:Ribosomal Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C041672:ribosomal protein L34; D004259:DNA-Directed DNA Polymerase; C083980:dnaN protein, Bacteria