title: Kinetic analysis of enzyme reactions with slow-binding inhibition.
abstract: In this paper we present a general kinetic study of slow-binding inhibition processes, i.e. enzyme reactions that do not respond instantly to the presence of a competitive inhibitor. The analysis that we present is based on the equation that describes the formation of products with time in each case on the experimental progress curve. It is carried out under the condition of limiting enzyme concentration and allows the discrimination between the different cases of slow-binding inhibition. The mechanism in which the formation of complex enzyme-inhibitor is a single or two slow steps or follow a rapid equilibrium, has been considered. The corresponding explicit equations of each case have been obtained and checked by numerical integration. A kinetic data analysis to evaluate the corresponding kinetic parameters is suggested. We illustrate the method, numerically by computer simulation, of the reaction and present some numerical examples that demonstrate the applicability of our procedure.
authors.affiliation: Departamento de Química-Física, Escuela Universitaria Politécnica, Universidad de Castilla-La Mancha, Albacete, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Garrido-del Solo
authors.forename: F
authors.initials: F
authors.lastname: García-Cánovas
authors.forename: B H
authors.initials: BH
authors.lastname: Havesteen
authors.forename: R V
authors.initials: RV
authors.lastname: Castellanos
mesh_terms: D003198:Computer Simulation; D004791:Enzyme Inhibitors; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes