title: Cutting edge: Role of C-C chemokine receptor 5 in organ-specific and innate immunity to Cryptococcus neoformans.
abstract: After intratracheal inoculation of the AIDS-associated pathogen Cryptococcus neoformans, 12-wk survival was >90% for CCR5+/+ mice but <25% for CCR5-/- mice. There were no defects in lung leukocyte recruitment (wk 5), pulmonary clearance, or delayed-type hypersensitivity in CCR5-/- mice. However, CCR5-/- mice had defects in leukocyte recruitment into the brain and, strikingly, in elimination of cryptococcal polysaccharide from the brain. In nonimmune CCR5-/- mice, there was a significant defect in macrophage recruitment after challenge with shed cryptococcal products (C. neoformans filtrate Ag) but not other nonspecific stimuli. Thus, CCR5 plays specific roles in innate immunity and organ-specific leukocyte trafficking during host defense against C. neoformans.
authors.affiliation: Pulmonary Division, Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA. ghuff@umich.edu
authors.forename: G B
authors.initials: GB
authors.lastname: Huffnagle
authors.forename: L K
authors.initials: LK
authors.lastname: McNeil
authors.forename: R A
authors.initials: RA
authors.lastname: McDonald
authors.forename: J W
authors.initials: JW
authors.lastname: Murphy
authors.forename: G B
authors.initials: GB
authors.lastname: Toews
authors.forename: N
authors.initials: N
authors.lastname: Maeda
authors.forename: W A
authors.initials: WA
authors.lastname: Kuziel
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D003453:Cryptococcosis / Q000235:genetics / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000276:immunology*; D007113:Immunity, Innate; D008172:Lung Diseases, Fungal / Q000235:genetics / Q000276:immunology / Q000401:mortality / Q000473:pathology; D016919:Meningitis, Cryptococcal / Q000235:genetics / Q000276:immunology / Q000401:mortality / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D019713:Receptors, CCR5 / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D019713:Receptors, CCR5