Direct HPLC analysis of ketoprofen in horse plasma applying an ADS-restricted access-phase.
Making up part of the unique family of restricted access materials (RAM) the Lichrospher ADS (alkyl-diol silica) sorbents have been developed as special packing materials for precolumns used for LC-integrated sample processing of biofluids. The advantage of such phases consists of direct injection of untreated biological fluids without sample clean-up and elimination of the protein matrix together with an on-column enrichment. The plasma samples, with internal standard phenacetin added (not essential), were brought onto the precolumn (C-18 ADS, 25 micron, 25 x 4 mm i.d.) using a phosphate buffer, 0.1 M, pH 7.0. After washing with the buffer, the ADS column was backflushed with the mobile phase phosphate buffer 0. 05 M pH 7.0: acetonitrile (80:20), thus transporting the analytes onto a reversed-phase column Ecocart 125-3 HPLC cartridge with a LiChrocart 4-4 guard column, both packed with LiChrospher 5 micron 100 RP-18; after separation detection was performed in UV at 260 nm. Essential features of the method include the novel precolumn packing, the absence of sample pretreatment, a quantitave recovery, good precision and accuracy, as well as a considerable reduction of analysis time compared to conventional manual methods applied in bioavailability studies.
Baeyens
W R
WR
Laboratory of Drug Quality Control, Faculty of Pharmaceutical Sciences, University of Ghent, Harelbekestraat 72, B-9000 Ghent, Belgium.
Van der Weken
G
G
Haustraete
J
J
Aboul-Enein
H Y
HY
Corveleyn
S
S
Remon
J P
JP
García-Campaña
A M
AM
Deprez
P
P
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006736:Horses; D007660:Ketoprofen / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D012822:Silicon Dioxide; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; C095072:alkyl-diol silica; D012822:Silicon Dioxide; D007660:Ketoprofen