title: Plasmodium falciparum field isolates commonly use erythrocyte invasion pathways that are independent of sialic acid residues of glycophorin A.
abstract: Erythrocyte invasion by malaria parasites is mediated by specific molecular interactions. Sialic acid residues of glycophorin A are used as invasion receptors by Plasmodium falciparum. In vitro invasion studies have demonstrated that some cloned P. falciparum lines can use alternate receptors independent of sialic acid residues of glycophorin A. It is not known if invasion by alternate pathways occurs commonly in the field. In this study, we used in vitro growth assays and erythrocyte invasion assays to determine the invasion phenotypes of 15 P. falciparum field isolates. Of the 15 field isolates tested, 5 multiply in both neuraminidase and trypsin-treated erythrocytes, 3 multiply in neuraminidase-treated but not trypsin-treated erythrocytes, and 4 multiply in trypsin-treated but not neuraminidase-treated erythrocytes; 12 of the 15 field isolates tested use alternate invasion pathways that are not dependent on sialic acid residues of glycophorin A. Alternate invasion pathways are thus commonly used by P. falciparum field isolates. Typing based on two polymorphic markers, MSP-1 and MSP-2, and two microsatellite markers suggests that only 1 of the 15 field isolates tested contains multiple parasite genotypes. Individual P. falciparum lines can thus use multiple invasion pathways in the field. These observations have important implications for malaria vaccine development efforts based on EBA-175, the P. falciparum protein that binds sialic acid residues of glycophorin A during invasion. It may be necessary to target parasite ligands responsible for the alternate invasion pathways in addition to EBA-175 to effectively block erythrocyte invasion by P. falciparum.
authors.affiliation: Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: J N
authors.initials: JN
authors.lastname: Okoyeh
authors.forename: C R
authors.initials: CR
authors.lastname: Pillai
authors.forename: C E
authors.initials: CE
authors.lastname: Chitnis
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000469:parasitology*; D005838:Genotype; D006021:Glycophorins / Q000502:physiology*; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000502:physiology*; D009439:Neuraminidase / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000502:physiology*; D016133:Polymerase Chain Reaction; D014357:Trypsin / Q000494:pharmacology
chemical_list: D006021:Glycophorins; D009439:Neuraminidase; D014357:Trypsin; D019158:N-Acetylneuraminic Acid