title: A mutant of gluconobacter oxydans deficient in gluconic acid dehydrogenase
abstract: Gluconobacter oxydans ATCC 9937 was subjected to transposon mutagenesis using Tn5. A non-pigmented mutant was shown to be defective in gluconic acid dehydrogenase and to produce gluconic acid from glucose, whereas the parent strain produced 2, 5-diketogluconic acid. Cloning and sequencing of the region containing the Tn5 insertion showed that the insertion point occurred in an open reading frame homologous (42% amino acid identity) to the ribF genes of Pseudomonas fluorescens and Escherichia coli. The resulting lack of a riboflavin cofactor would explain the loss of enzyme activity.
authors.affiliation: Division of Biotechnology, Regional Research Laboratory, Canal Road, Jammu Tawi, India.
authors.initials: A
authors.lastname: Gupta
authors.initials: M
authors.lastname: Felder
authors.initials: V
authors.lastname: Verma
authors.initials: J
authors.lastname: Cullum
authors.initials: GN
authors.lastname: Qazi