title: Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components.
abstract: OBJECTIVE
  To quantify the cellular isoform of prion protein (PrP(c)) in human blood using a new time-resolved dissociation-enhanced fluoroimmunoassay (DELFIA).
  
  
  METHODS
  The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP(c) were analysed.
  
  
  RESULTS
  26. 5% of blood PrP(c) was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations).
  
  
  CONCLUSIONS
  The majority of blood PrP(c) is found in the platelet and plasma compartments.
authors.affiliation: National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, UK. ian.macgregor@snbts.csa.scot.nhs.uk
authors.forename: I
authors.initials: I
authors.lastname: MacGregor
authors.forename: J
authors.initials: J
authors.lastname: Hope
authors.forename: G
authors.initials: G
authors.lastname: Barnard
authors.forename: L
authors.initials: L
authors.lastname: Kirby
authors.forename: O
authors.initials: O
authors.lastname: Drummond
authors.forename: D
authors.initials: D
authors.lastname: Pepper
authors.forename: V
authors.initials: V
authors.lastname: Hornsey
authors.forename: R
authors.initials: R
authors.lastname: Barclay
authors.forename: H
authors.initials: H
authors.lastname: Bessos
authors.forename: M
authors.initials: M
authors.lastname: Turner
authors.forename: C
authors.initials: C
authors.lastname: Prowse
mesh_terms: D000941:Antigens; D001792:Blood Platelets / Q000737:chemistry; D004355:Drug Stability; D004912:Erythrocytes / Q000737:chemistry; D015200:Fluoroimmunoassay / Q000379:methods; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D009504:Neutrophils / Q000737:chemistry; D010949:Plasma / Q000737:chemistry; D011328:Prions / Q000097:blood* / Q000737:chemistry / Q000276:immunology
chemical_list: D000941:Antigens; D011328:Prions