The genomic and sequence analysis of rat histone H2B genes.
In addition to previously characterized testis-specific and somatic H2B histone genes, two somatic histone H2B genes, hereafter called sH2B-2 and sH2B-3, were isolated from a rat genomic library, genomic organization was determined, and their promoter was sequenced. Like many other H2B genes, sH2B-2 gene was closely linked to H2A gene whereas H3 gene was located upstream of sH2B-3 gene. Deletion and mutation analysis of 5' sequence fused to CAT reporter gene revealed that the interaction between CCAAT and octamer binding factors is important for S-phase-specific activation of sH2B-3 gene.
Choi
Y C
YC
Jeil Premium 1209, Pohang, Gyeongbuk, Korea.
Chae
C B
CB
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004252:DNA Mutational Analysis; D015698:Genomic Library; D006657:Histones / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D051381:Rats; D017422:Sequence Analysis, DNA; D014162:Transfection
D006657:Histones; D015500:Chloramphenicol O-Acetyltransferase