Structure of a new acidic O-antigen of Proteus vulgaris O22 containing O-acetylated 3-acetamido-3,6-dideoxy-D-glucose.
The acidic O-specific polysaccharide of Proteus vulgaris O22 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected 1H, 13C heteronuclear multiple-quantum coherence (HMQC) experiments, and the following structure for the branched pentasaccharide repeating unit was established: [sequence: see text] where Qui3NAc is 3-acetamido-3,6-dideoxyglucose, O-acetylation of QuiNAc at position 4 is stoichiometric and at position 2 nonstoichiometric. Serological relationships of P. vulgaris O22 with some other Proteus strains were substantiated on the level of the O-antigen structures.
Toukach
F V
FV
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Bartodziejska
B
B
Senchenkova
S N
SN
Wykrota
M
M
Shashkov
A S
AS
Rozalski
A
A
Knirel
Y A
YA
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D006461:Hemolysis; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011511:Proteus / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry / Q000276:immunology*
D019081:O Antigens; D009844:Oligosaccharides; C033655:3-acetamido-3,6-dideoxyglucose; D000117:Acetylglucosamine