title: Effect of liquorice and glycyrrhizin on rat liver carcinogen metabolizing enzymes.
abstract: We investigated the effect of single or repeated intake of conspicuous amounts of licorice root extract (LE, 3138 or 6276 mg/kg body weight (bw) per os) or its natural constituent glycyrrhizin (G, 240 or 480 mg/kg bw per os) on Sprague-Dawley rat liver monooxygenases. Whereas a single LE or G dose was unable to affect CYP superfamily, four daily doses induced CYP3A, CYP1A2 and to varying extents CYP2B1-linked monooxygenases. A boosting effect on testosterone 6beta- (CYP3A1/2, CYP1A1/2), 7alpha- (CYP1A1/2, CYP2A1), 16alpha- (CYP2B1, CYP2C11), 2alpha- (CYP2C11) and 2beta- (CYP3A1, CYP1A1) -dependent oxidases as well as on androst-4-ene-3,17-dione- (CYP3A1/2) -supported monooxygenases were also achieved. Harmful outcomes associated to CYP changes (e.g. cotoxicity, cocarcinogenicity and promotion) may be of concern.
authors.affiliation: Department of Pharmacology, Biochemical Toxicology Unit, University of Bologna, Italy. paolini@biocfarm.unibo.it
authors.forename: M
authors.initials: M
authors.lastname: Paolini
authors.forename: J
authors.initials: J
authors.lastname: Barillari
authors.forename: M
authors.initials: M
authors.lastname: Broccoli
authors.forename: L
authors.initials: L
authors.lastname: Pozzetti
authors.forename: P
authors.initials: P
authors.lastname: Perocco
authors.forename: G
authors.initials: G
authors.lastname: Cantelli-Forti
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D002273:Carcinogens / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D005260:Female; D006035:Glycyrrhiza; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D006900:Hydroxylation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; D019695:Glycyrrhizic Acid; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C465138:Cyp3a23-3a1 protein, rat; D051544:Cytochrome P-450 CYP3A