Neutrophils from patients with advanced human immunodeficiency virus infection have impaired complement receptor function and preserved Fcgamma receptor function.
Interleukin (IL)-8 production by human polymorphonuclear leukocytes (PMNL) to Cryptococcus neoformans is related to complement activation. Generation of the bioactive fragments C3a and C5a is responsible for IL-8 release. IL-8 production was analyzed in response to C. neoformans by PMNL from persons with early- and late-stage (>400 and <200 CD4 cells/mm3, respectively) human immunodeficiency virus (HIV) infection who were at high risk for cryptococcosis. IL-8 release by PMNL from persons with early-stage infection and from healthy donors was similar; however, PMNL from persons with late-stage HIV infection had significantly impaired IL-8 production, which correlated with reduced IL-8 response to C3a and C5a proteins and decreased CD88 expression. Addition of murine monoclonal antibody (MAb) 18B7 promoted phagocytosis and restored IL-8 release consistent with integrity of FcgammaRIII. These results provide evidence for a selective defect in CD88 expression on PMNL from persons with late-stage HIV infection. However, Fcgamma receptor expression in PMNL appears to be intact and allows MAb to glucuronoxylomannan to positively influence PMNL function.
Monari
C
C
Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy.
Casadevall
A
A
Pietrella
D
D
Bistoni
F
F
Vecchiarelli
A
A
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D015926:Complement C3a / Q000276:immunology; D015936:Complement C5a / Q000276:immunology; D003455:Cryptococcus neoformans / Q000276:immunology*; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D009504:Neutrophils / Q000276:immunology*; D011951:Receptors, Complement / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D011951:Receptors, Complement; D017452:Receptors, IgG; D015926:Complement C3a; D015936:Complement C5a