title: Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in Listeria monocytogenes.
abstract: lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol concentrations, and a significant reduction in virulence.
authors.affiliation: Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: P D
authors.initials: PD
authors.lastname: Cotter
authors.forename: N
authors.initials: N
authors.lastname: Emerson
authors.forename: C G
authors.initials: CG
authors.lastname: Gahan
authors.forename: C
authors.initials: C
authors.lastname: Hill
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015169:Colony Count, Microbial; D017353:Gene Deletion; D005798:Genes, Bacterial; D018869:Heat-Shock Response; D000071677:Histidine Kinase; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase