title: Chiral separations in capillary high-performance liquid chromatography and nonaqueous capillary electrochromatography using helically chiral poly(diphenyl-2-pyridylmethyl methacrylate) as chiral stationary phase.
abstract: Enantioseparations in nonaqueous capillary electrochromatography (CEC) are reported in this study for the first time, using wide-pore aminopropyl silica gel coated with helically chiral poly(diphenyl-2-pyridylmethyl methacrylate) (PDPM) as chiral stationary phase (CSP). The anodic electroosmotic flow (EOF) in a methanolic solution of ammonium acetate was used for the migration of neutral analytes through the packed bed in the capillaries. Four different techniques, high-performance liquid chromatography (HPLC) in common-size columns, capillary HPLC, pressure-assisted CEC and CEC were compared from the viewpoint of separation parameters. The latter three were performed with the same experimental setup, varying the relative contribution of the pressure-driven and the electrically driven flow to the overall mobility of the analyte. Capillary HPLC offers clear advantages compared to enantioseparations in common-size columns. However, for a given particle size of the packing material, CEC was not obviously advantageous compared to pressure-driven separations.
authors.affiliation: Institute of Pharmaceutical Chemistry, University of Münster, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Krause
authors.forename: B
authors.initials: B
authors.lastname: Chankvetadze
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: G
authors.initials: G
authors.lastname: Blaschke
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry*; D011312:Pressure; D011725:Pyridines / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D008689:Methacrylates; D011725:Pyridines; C400644:poly(diphenyl-2-pyridylmethyl methacrylate)