title: Ultrasound-guided tissue-core biopsy of liver, spleen and kidney in normal dogs.
abstract: Six normal dogs were subjected to ultrasound-guided biopsy of the liver, spleen and kidney to examine the accuracy of the technique (i.e. the presence of targeted tissue) and the histologic quality of the biopsies. Five consecutive tissue-core biopsies of each organ were taken on one or more occasions. The accuracy of the technique was 77% for hepatic, 90% for splenic, 53.5% for left kidney and 40% for right kidney biopsies. The histologic quality of the liver and kidney samples was sufficient, although for some samples the diagnostic value was limited by their size and in renal samples either cortical or medullary tissue was sometimes lacking. In contrast, the quality of the splenic sections was not good. The effect of reused and resterilized needles on the quality of the specimens was evaluated by histologic inspection of the samples and by the amount of biopsies lacking tissue. All tissue samples, including those taken with reused or resterilized needles had sharp-cut edges. Twenty-two of the total number of 120 biopsies (18%) contained no tissue. Absence of tissue in the samples was observed in biopsies taken with all needle types. The animals were observed for possible complications of the repeated needle biopsy. Apart from one case of hematuria, no complications were encountered.
authors.affiliation: Department of Medical Imaging, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
authors.forename: L M
authors.initials: LM
authors.lastname: de Rycke
authors.forename: H J
authors.initials: HJ
authors.lastname: van Bree
authors.forename: P J
authors.initials: PJ
authors.lastname: Simoens
mesh_terms: D000818:Animals; D001707:Biopsy, Needle / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000033:anatomy & histology*; D007668:Kidney / Q000166:cytology*; D008099:Liver / Q000166:cytology*; D009339:Needles; D013154:Spleen / Q000166:cytology*; D018084:Ultrasonography, Interventional / Q000662:veterinary*