title: Effect of small bowel bacterial overgrowth on the immunogenicity of single-dose live oral cholera vaccine CVD 103-HgR.
abstract: Several live oral vaccines (polio, bovine rotavirus, CVD 103-HgR cholera) are less immunogenic in developing than in industrialized countries. It was hypothesized that proximal small bowel bacterial overgrowth (common in children in less developed countries but rare in industrialized settings) diminishes the vibriocidal antibody response to CVD 103-HgR. In total, 202 fasting Santiago schoolchildren aged 5-9 years had lactulose breath H2 tests to detect proximal small bowel bacteria 1 day before ingesting CVD 103-HgR. Florid small bowel overgrowth was observed in 10 (5.6%) of 178 analyzable children. In children with florid overgrowth, vibriocidal seroconversion differed little from other children (60% vs. 67%), but the geometric mean titer was lower (160 vs. 368; P=.25). By logistic regression, increased peak breath H2 at small bowel time points was associated with diminished seroconversion (P=.04), as was the interaction of H2 value and weight (children >25 kg had lower seroconversion rates among subjects with heaviest overgrowth).
authors.affiliation: Centro para Vacunas en Desarrollo-Chile; Roberto del Rio Children's Hospital, University of Chile, Campus Oriente, Santiago, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Lagos
authors.forename: A
authors.initials: A
authors.lastname: Fasano
authors.forename: S S
authors.initials: SS
authors.lastname: Wasserman
authors.forename: V
authors.initials: V
authors.lastname: Prado
authors.forename: O
authors.initials: O
authors.lastname: San Martin
authors.forename: P
authors.initials: P
authors.lastname: Abrego
authors.forename: G A
authors.initials: GA
authors.lastname: Losonsky
authors.forename: S
authors.initials: S
authors.lastname: Alegria
authors.forename: M M
authors.initials: MM
authors.lastname: Levine
mesh_terms: D000284:Administration, Oral; D000907:Antibodies, Bacterial / Q000097:blood*; D001944:Breath Tests; D002648:Child; D002675:Child, Preschool; D022121:Cholera Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D005260:Female; D006801:Humans; D006859:Hydrogen / Q000032:analysis; D007421:Intestine, Small / Q000382:microbiology*; D007792:Lactulose / Q000378:metabolism; D008297:Male; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D022121:Cholera Vaccines; D007792:Lactulose; D006859:Hydrogen