title: Molecular taxonomy of Trichophyton mentagrophytes and T. tonsurans.
abstract: Most members of the anamorph genus Trichophyton are anthropophilic and have evolved with the human host. Classical parameters for the identification of dermatophytes include clinical features, cultural characteristics, conidial morphology and physiological test results. Phenotypic variability and pleomorphism due to culturing on artificial media is common among this group of organisms and has led to the description of numerous species. The validity of taxa around T. mentagrophytes and T. tonsurans was verified. Morphological and physiological features were compared to results of three different molecular techniques (sequencing of the internal transcribed spacer (ITS) region of the ribosomal operon, PCR fingerprinting and amplified fragment length polymorphism (AFLP) analysis). Twenty-four species or varieties investigated could be reduced to five taxa and were reclassified or synonymized as Trichophyton tonsurans, T. interdigitale, T. mentagrophytes, T. simii and T. erinacei.
authors.affiliation: Institute of Microbiology and Hygiene, Department of Parasitology (Charité), Humboldt University, Berlin, Germany. yvonne.graeser@charite.de
authors.forename: Y
authors.initials: Y
authors.lastname: Gräser
authors.forename: A F
authors.initials: AF
authors.lastname: Kuijpers
authors.forename: W
authors.initials: W
authors.lastname: Presber
authors.forename: G S
authors.initials: GS
authors.lastname: De Hoog
mesh_terms: D000818:Animals; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D006801:Humans; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014005:Tinea / Q000382:microbiology*; D014249:Trichophyton / Q000145:classification* / Q000235:genetics*
chemical_list: D004271:DNA, Fungal