title: A macaque model for studying mechanisms controlling oocyte development and maturation in human and non-human primates.
abstract: A model to study mechanisms controlling nuclear and cytoplasmic maturation of primate oocytes is being developed in our laboratory. The high incidence of pregnancy failure in women following in-vitro fertilization (IVF) may be partly attributed to inadequate cytoplasmic maturation of oocytes. Advancement of knowledge of mechanisms controlling primate oocyte maturation would have important implications for treatment of human infertility, and would potentially increase numbers of viable non-human primate embryos for biomedical research. Use of a non-human primate model to study oocyte and embryo biology avoids legal, ethical and experimental limitations encountered in a clinical situation. Using this model, the meiotic and developmental capacity of oocytes from three sources have been compared: (i) in-vivo matured oocytes from monkeys stimulated with follicle-stimulating hormone (FSH) and human chorionic gonadotrophin, (ii) in-vitro matured oocytes from monkeys primed with FSH, and (iii) in-vitro matured oocytes from non-stimulated monkeys. This work demonstrates that oocyte developmental competence is likely acquired both during follicle development, before meiotic resumption, and during meiotic progression, concurrent with nuclear maturation. Potential causes of developmental failure of in-vitro matured oocytes, implications for human infertility, and future strategies to study the regulation of primate oocyte maturation are discussed.
authors.affiliation: Wisconsin Regional Primate Research Center, 1223 Capitol Court, Madison, WI 53715, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Schramm
authors.forename: B D
authors.initials: BD
authors.lastname: Bavister
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000627:therapeutic use; D006801:Humans; D008253:Macaca mulatta; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D013268:Stimulation, Chemical; D016896:Treatment Outcome
chemical_list: D005640:Follicle Stimulating Hormone