Cloning of Schizosaccharomyces pombe bio2 by heterologous complementation of a Saccharomyces cerevisiae mutant.
A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterization of a novel bio gene: bio2, encoding biotin synthase from Schizosaccharomyces pombe.
Phalip
V
V
Laboratoire de Biotechnologie, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brandt, 67400 Illkirch-Graffenstaden, France.
Lemoine
Y
Y
Jeltsch
J M
JM
D001710:Biotin / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*; D013466:Sulfurtransferases / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D001710:Biotin; D013466:Sulfurtransferases; C057985:biotin synthetase