Symmetry of initial cell divisions among primitive hematopoietic progenitors is independent of ontogenic age and regulatory molecules.
We have developed a time-lapse camera system to follow the replication history and the fate of hematopoietic stem cells (HSC) at a single-cell level. Combined with single-cell culture, we correlated the early replication behavior with colony development after 14 days. The membrane dye PKH26 was used to monitor cell division. In addition to multiple, synchronous, and symmetric divisions, single-sorted CD34(+)/CD38(-) cells derived from fetal liver (FLV) also gave rise to a daughter cell that remained quiescent for up to 8 days, whereas the other daughter cell proliferated exponentially. Upon separation and replating as single cells onto medium containing a cytokine cocktail, 60.6% +/- 9.8% of the initially quiescent cells (PKH26 bright) gave rise again to colonies and 15.8% +/- 7.8% to blast colonies that could be replated. We have then determined the effects of various regulatory molecules on symmetry of initial cell divisions. After single-cell sorting, the CD34(+)/CD38(-) cells derived from FLV were exposed to flt3-ligand, thrombopoietin, stem cell factor (SCF), or medium containing a cytokine cocktail (with SCF, interleukin-3, interleukin-6, granulocyte-macrophage colony-stimulating factor, and erythropoietin). Whereas mitotic rate, colony efficiency, and asymmetric divisions could be altered using various regulatory molecules, the asymmetric division index, defined as the number of asymmetric divisions versus the number of dividing cells, was not altered significantly. This observation suggests that, although lineage commitment and cell proliferation can be skewed by extrinsic signaling, symmetry of early divisions is probably under the control of intrinsic factors.
Huang
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Departments of Medicine and Bioengineering, University of California, San Diego, CA, USA.
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Francis
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Palsson
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D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004396:Coloring Agents; D004357:Drug Synergism; D004921:Erythropoietin / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology; D005333:Fetus / Q000166:cytology; D005456:Fluorescent Dyes; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006413:Hematopoietic System / Q000196:embryology / Q000254:growth & development*; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D008565:Membrane Proteins / Q000494:pharmacology; D018715:Microscopy, Video; D008940:Mitotic Index; D009930:Organic Chemicals; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology; D013269:Stochastic Processes; D013926:Thrombopoietin / Q000494:pharmacology
D018952:Antigens, CD34; D004396:Coloring Agents; D005456:Fluorescent Dyes; D016298:Hematopoietic Cell Growth Factors; D007378:Interleukins; D008565:Membrane Proteins; D009930:Organic Chemicals; C070080:PKH 26; D011994:Recombinant Proteins; D019089:Stem Cell Factor; C084881:flt3 ligand protein; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin