title: CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms.
abstract: The transcription factor CCAAT/enhancer binding protein beta, C/EBPbeta, plays a significant role in the regulation of hepatocyte growth and differentiation. A single mRNA coding for C/EBPbeta produces several protein isoforms. Two pathways for generation of low molecular weight C/EBPbeta isoforms have been described: specific proteolytic cleavage and initiation of translation from different AUG codons of C/EBPbeta mRNA. A truncated C/EBPbeta isoform, LIP, is induced in rat livers in response to partial hepatectomy (PH) via the alternative translation mechanism. Here we present evidence that CUG repeat binding protein, CUGBP1, interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms. Two binding sites for CUGBP1 are located side by side between the first and second AUG codons of C/EBPbeta mRNA. One binding site is observed in an out of frame short open reading frame (sORF) that has been previously shown to regulate initiation of translation from different AUG codons of C/EBPbeta mRNA. Analysis of cytoplasmic and polysomal proteins from rat liver after PH showed that CUGBP1 is associated with polysomes that translate low molecular weight isoforms of C/EBPbeta. The binding activity of CUGBP1 to the 5' region of C/EBPbeta mRNA shows increased association with these polysomal fractions after PH. Addition of CUGBP1 into a cell-free translation system leads to increased translation of low molecular weight isoforms of C/EBPbeta. Our data demonstrate that CUGBP1 protein is an important component for the regulation of initiation from different AUG codons of C/EBPbeta mRNA.
authors.affiliation: Huffington Center on Aging, Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA. nikolait@bcm.tmc.edu
authors.forename: N A
authors.initials: NA
authors.lastname: Timchenko
authors.forename: A L
authors.initials: AL
authors.lastname: Welm
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: L T
authors.initials: LT
authors.lastname: Timchenko
mesh_terms: D020121:5' Untranslated Regions / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D022782:CCAAT-Enhancer-Binding Protein-beta; D022762:CCAAT-Enhancer-Binding Proteins; D000067879:CELF1 Protein; D002474:Cell-Free System; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008115:Liver Regeneration; D009687:Nuclear Proteins / Q000235:genetics*; D016366:Open Reading Frames; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics; D012261:Ribonucleoproteins / Q000378:metabolism*; D018911:Trinucleotide Repeats / Q000502:physiology
chemical_list: D020121:5' Untranslated Regions; D022782:CCAAT-Enhancer-Binding Protein-beta; D022762:CCAAT-Enhancer-Binding Proteins; D000067879:CELF1 Protein; C102843:CELF1 protein, human; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins