Genomic analysis of male germ cell-specific actin capping protein alpha.
The Gsg3 gene which expresses specifically in haploid germ cells is a mouse testicular homolog of somatic cell type actin capping protein alpha (ACP alpha). We have obtained a mouse Gsg3 genomic clone using cDNA as a probe. Sequencing data showed that the Gsg3 gene was not interrupted by introns. The transcription initiation site of the gene was preceded not by a TATA box or GC rich promoter motifs, but by two consensus cAMP-response element (CRE) motifs at the putative position. Southern blotting analysis showed that Gsg3 is a single copy gene in the mouse, and conserved in mammals. Phylogenetic analysis showed that Gsg3 is a novel ACP alpha specific for haploid germ cells.
Yoshimura
Y
Y
Department of Science for Laboratory Animal Experimentation, Osaka University, Japan.
Tanaka
H
H
Nozaki
M
M
Yomogida
K
K
Shimamura
K
K
Yasunaga
T
T
Nishimune
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D051347:CapZ Actin Capping Protein; D003001:Cloning, Molecular; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013094:Spermatozoa / Q000502:physiology*; D013735:Testicular Hormones / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
C090518:CAPZA3 protein, mouse; D051347:CapZ Actin Capping Protein; D013735:Testicular Hormones