Sequence and expression of the monkey homologue of the ER-golgi intermediate compartment lectin, ERGIC-53.
We obtained the cDNA sequence of the monkey homologue of the intermediate compartment protein ERGIC-53 by both cDNA library screening and RT-PCR amplification. The final sequence of 2422 nts of the monkey ERGIC-53 cDNA is 96.2% identical to the human ERGIC-53 cDNA and 87% and 67% identical to the rat and amphibian cDNA, respectively. The translated CV1 ERGIC-53 protein is 96.47% identical to the human ERGIC-53, 87% identical to the rat p58 and 66. 98% to the Xenopus laevis protein. Southern blot analysis of multiple genomic DNAs shows the presence of sequences similar to ERGIC-53 in different species. ERGIC-53 is expressed as a major transcript of about 5.5 kb in either monkey CV1 or in human CaCo2. A shorter transcript of 2.3 kb was detected in both cell lines and in mRNAs derived from human pancreas and placenta.
Sarnataro
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Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli 'Federico II', via S. Pansini 5, 80131, Naples, Italy.
Caporaso
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Bonatti
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Remondelli
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000882:Haplorhini; D006801:Humans; D037102:Lectins; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D051381:Rats; D017385:Sequence Homology
C084004:LMAN1 protein, human; D037102:Lectins; C501458:Lman1 protein, rat; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins