title: The influence of hydroxyapatite granules on the healing of a segmental defect filled with autologous bone marrow.
abstract: Hydroxyapatite(HA) ceramics are frequently used as a bone graft substitutes for the filling of bony defects. The addition of autologous bone marrow to HA ceramics does improve defect healing. There is conflicting evidence in the literature whether autologous bone marrow transplantation alone is as effective as the combination of HA ceramics and bone marrow combined. It was the purpose of this study to identify the role of additional HA ceramic granules on the healing of a sheep tibia segmental defect filled with autologous bone marrow. After permission of the local animal rights committee was obtained, a 3 cm segmental defect in the midshaft of 31 adult sheep was stabilized with an unreamed tibia nail. The animals were divided into 4 groups according to the mode of defect filling: HA plus autologous bone marrow (HA + MAR) (n = 8), autologous bone marrow (MAR) (n = 9), empty defect (DEF) (n = 6), cancellous bone graft (CAN) (n = 8). After three months follow up animals were sacrificed and analysed for the key parameters of union and maximum torque at failure. One nonunion was present in each of the HA + MAR, MAR, and CAN groups. Four of the six animals in the DEF group developed a nonunion. Maximum torque at failure was reported as percentage of the intact contralateral tibia: HA + MAR 39% +/- 24%, MAR 26% +/- 17%, DEF 22% +/- 13%, CAN 41% +/- 20%. The difference between the groups was statistically significant, but appeared to be relevant. We conclude from our data, that HA ceramics do improve healing of a segmental defect in the sheep tibia filled with autologous bone marrow. The results of this combination are comparable to cancellous autograft.
authors.affiliation: Department of Trauma Surgery, Hannover Medical School, Germany. bwippermann@hotmail.com
authors.forename: B
authors.initials: B
authors.lastname: Wippermann
authors.forename: C
authors.initials: C
authors.lastname: Donow
authors.forename: H E
authors.initials: HE
authors.lastname: Schratt
authors.forename: F C
authors.initials: FC
authors.lastname: den Boer
authors.forename: T
authors.initials: T
authors.lastname: Blokhuis
authors.forename: P
authors.initials: P
authors.lastname: Patka
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000033:anatomy & histology / Q000000981:diagnostic imaging; D002516:Ceramics; D017886:Durapatite / Q000627:therapeutic use*; D008852:Microradiography; D012756:Sheep; D014182:Transplantation, Autologous
chemical_list: D017886:Durapatite