Ascorbate biosynthesis in Arabidopsis cell suspension culture.
The biosynthesis of L-ascorbic acid (L-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of L-AA. Our results support the recently published biosynthetic pathway of L-AA from L-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365-369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading to L-AA. The possible physiological significance of these alternate routes is discussed.
Davey
M W
MW
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. madav@gengenp.rug.ac.be
Gilot
C
C
Persiau
G
G
Ostergaard
J
J
Han
Y
Y
Bauw
G C
GC
Van Montagu
M C
MC
D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D001205:Ascorbic Acid / Q000096:biosynthesis*; D002478:Cells, Cultured; D005690:Galactose / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical
D001205:Ascorbic Acid; D005690:Galactose