Reciprocal changes in the expression of neurotrophin mRNAs in target tissues and peripheral nerves of aged rats.
trk receptors are downregulated in both dorsal root ganglion (DRG) and spinal motoneurons of aged rats with behavioral sensorimotor deficits. Here we provide evidence, using reverse transcription-polymerase chain reaction (RT-PCR), of decreased levels of neurotrophin (nerve growth factor, NGF; brain-derived neurotrophic factor, BDNF; neurotrophin-3, NT-3; and neurotrophin-4, NT-4) mRNAs in target muscles. Moreover, the degree of neurotrophin mRNA decrease in target muscles seems to co-vary with the extent of sensorimotor disturbances. In contrast, the peripheral nerve of aged rats showed a reciprocal regulation of neurotrophins, with increased levels of NGF, BDNF, and NT-4 mRNAs. Taken together, evidence suggest an aging-related attenuation of neurotrophin signaling between target tissues, on one hand, and DRG and motoneurons, on the other, and, furthermore, that target-derived neurotrophins regulate the expression levels of trk mRNAs in both DRG and motoneurons.
Ming
Y
Y
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Bergman
E
E
Edström
E
E
Ulfhake
B
B
D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D010525:Peripheral Nerves / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism
D009414:Nerve Growth Factors; D012333:RNA, Messenger