[Functional interactions of components of the adenylate cyclase system in the rat liver after prenatal exposure to gamma irradiation].
The effects of long-term prenatal gamma-radiation (0.5 Gy) on the glucagon signalling through adenylyl cyclase in adult rat liver have been investigated. The coupling of receptor/Gs-protein/adenylyl cyclase was tested using kinetic constants for stimulation of adenylyl cyclase by GTP, Gp(NH)pp, AlF4- and glucagon. The estimated data suggests that prenatal chronic gamma-radiation prompted (i) a decrease in rate of GTP hydrolysis on Gs-protein; (ii) a reduction of glucagon potency to accelerate the exchange GDP for GTP on Gs-protein.
Chubanov
V S
VS
Department of Biochemistry, Faculty of Biology, Belarus State University, Minsk. biochem@bio.bsu.unibel.by
Rogov
Iu I
IuI
Konoplia
E F
EF
Sholukh
M V
MV
D000262:Adenylyl Cyclases / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D002588:Cesium Radioisotopes; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D005720:Gamma Rays; D005934:Glucagon / Q000378:metabolism; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism* / Q000528:radiation effects*; D018811:Maternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D011829:Radiation Dosage; D011863:Radioimmunoassay; D051381:Rats; D018027:Receptors, Glucagon / Q000378:metabolism
D002588:Cesium Radioisotopes; D018027:Receptors, Glucagon; D005934:Glucagon; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases