Carotenoid hydroxylase from Haematococcus pluvialis: cDNA sequence, regulation and functional complementation.
A cDNA homologous to beta-carotene hydroxylase from Arabidopsis thaliana was isolated from the green alga Haematococcus pluvialis. The predicted amino acid sequence for this enzyme shows homology to the three known plant beta-carotene hydroxylases from Arabidopsis thaliana and from Capsicum annuum (38% identity) and to prokaryote carotenoid hydroxylases (32-34% identities). Heterologous complementation using E. coli strains which were genetically engineered to produce carotenoids indicated that the H. pluvialis beta-carotene hydroxylase was able to catalyse not only the conversion of beta-carotene to zeaxanthin but also the conversion of canthaxanthin to astaxanthin. Furthermore, Northern blot analysis revealed increased beta-carotene hydroxylase mRNA steady state levels after induction of astaxanthin biosynthesis. In accordance with the latter results, it is proposed that the carotenoid hydroxylase characterized in the present publication is involved in the biosynthesis of astaxanthin during cyst cell formation of H. pluvialis.
Linden
H
H
Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Germany. hartmut.linden@uni-konstanz.de
D000595:Amino Acid Sequence; D001483:Base Sequence; D016644:Canthaxanthin / Q000378:metabolism; D000460:Chlorophyta / Q000201:enzymology* / Q000235:genetics; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000302:isolation & purification; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives / Q000096:biosynthesis
D018076:DNA, Complementary; D012333:RNA, Messenger; D024341:Xanthophylls; D019207:beta Carotene; D016644:Canthaxanthin; C005948:astaxanthine; D006899:Mixed Function Oxygenases; C079232:beta-carotene hydroxylase