title: A resource of mapped human bacterial artificial chromosome clones.
abstract: To date, despite the increasing number of genomic tools, there is no repository of ordered human BAC clones that covers entire chromosomes. This project presents a resource of mapped large DNA fragments that span eight human chromosomes at approximately 1-Mb resolution. These DNA fragments are bacterial artificial chromosome (BAC) clones anchored to sequence tagged site (STS) markers. This clone collection, which currently contains 759 mapped clones, is useful in a wide range of applications from microarray-based gene mapping to identification of chromosomal mutations. In addition to the clones themselves, we describe a database, GenMapDB (http://genomics.med.upenn.edu/genmapdb), that contains information about each clone in our collection.
authors.affiliation: Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA. vcheung@mail.med.upenn.edu
authors.forename: V G
authors.initials: VG
authors.lastname: Cheung
authors.forename: H L
authors.initials: HL
authors.lastname: Dalrymple
authors.forename: S
authors.initials: S
authors.lastname: Narasimhan
authors.forename: J
authors.initials: J
authors.lastname: Watts
authors.forename: G
authors.initials: G
authors.lastname: Schuler
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
authors.forename: M
authors.initials: M
authors.lastname: Morley
authors.forename: A
authors.initials: A
authors.lastname: Bruzel
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D016208:Databases, Factual; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020407:Internet; D009693:Nucleic Acid Hybridization; D020161:Physical Chromosome Mapping / Q000379:methods*; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites