A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents.
We have developed a rapid and sensitive radioimmunoassay for the measurement of paclitaxel and related taxanes in biological specimens using commercially available reagents. The presence of paclitaxel competitively inhibited the binding of radioactive paclitaxel to polyclonal anti-taxane antibody or anti-taxane monoclonal antibody. When using polyclonal antibody, this method detected paclitaxel in concentrations as low as 0.87 nM and was also effective in 20% methanol. This radioimmunoassay is useful for the measurement of paclitaxel and taxanes in biological specimens like culture medium of paclitaxel-producing microorganisms and may be useful for the measurement of paclitaxel and related compounds in other potential natural sources.
Guillemard
V
V
Department of Medical Biology, Faculty of Medicine, Laval University, Sainte-Foy, Quebec G1K 7P4, Canada.
Landry
N
N
Pagé
M
M
D000818:Animals; D000906:Antibodies; D001952:Bridged-Ring Compounds / Q000032:analysis* / Q000276:immunology; D006801:Humans; D017239:Paclitaxel / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D043823:Taxoids
D000906:Antibodies; D001952:Bridged-Ring Compounds; D043823:Taxoids; C080625:taxane; D017239:Paclitaxel