Preparation and preliminary study of crystals of the recombinant calcium-regulated photoprotein obelin from the bioluminescent hydroid Obelia longissima.
Crystals of recombinant obelin, the Ca(2+)-regulated photoprotein from the marine hydroid Obelia longissima, have been grown from sodium citrate solutions. Crystals grow as hexagonal light-yellow rods (0.1 x 0.1 x 1.0 mm) which diffract to beyond 1.8 A with synchrotron radiation of 1.0 A wavelength. The crystals have a primitive hexagonal lattice with unit-cell parameters a = 81.55, c = 86.95 A. The asymmetric unit contains two molecules. This represents the successful preparation of single crystals of a photoprotein obelin which have promising diffraction properties.
Vysotski
E S
ES
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russia.
Liu
Z J
ZJ
Rose
J
J
Wang
B C
BC
Lee
J
J
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003460:Crystallization; D018360:Crystallography, X-Ray; D006829:Hydra; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
D008164:Luminescent Proteins; D011994:Recombinant Proteins; C010497:obelin; D002118:Calcium