[Detection of human papillomavirus DNA by molecular hybridization in tube: interest in cervical neoplasia].
The presence of human papillomavirus (HPV) DNA in 79 cervical specimens obtained from 70 patients was studied by using a molecular hybridization technique performed in tube. The results were compared to those of the cytological and histological studies. The molecular hybridization technique in tube (Hybrid Capture I) detects two groups of HPV types. One group is highly associated with the development of cancer (types 16, 18, 31, 33, 35, 45, 51, 52, 56) whereas the second group (types 6, 11, 42, 43, 44) is not. Among 42 patients with cervical lesions before any treatment, high risk DNA of HPV was found in 50% of those with low grade cytology and 90% with high grade cytology. In total, 32 out of the 42 patients (76%) who presented histological lesions, were actually infected by HPV. Samples were obtained before and after treatment from 9 patients. Seven out of 9 presented high grade cervical intraepithelial neoplasia (CIN) and 2 other patients had low grade CIN. HPV DNA was not detected in any of the patients after treatment. Detection of HPV DNA by molecular hybridization in tube is simple, sensitive, standardized, inexpensive and is well adapted to screening programs. It can be used in complement of the cytological diagnosis, in the surveillance of equivocal cytological abnormalities, and in the follow-up of treated patients.
Bellagra
N
N
Laboratoire de virologie, Institut Gernez-Rieux, CHU, 59037 Lille Cedex.
Hober
D
D
Idrissi
Y
Y
Dewilde
A
A
Laussel Reira
A C
AC
Boman
F
F
Leroy
J L
JL
Wattré
P
P
D000230:Adenocarcinoma / Q000473:pathology / Q000628:therapy / Q000821:virology; D000368:Aged; D003218:Condylomata Acuminata / Q000473:pathology / Q000628:therapy / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D005831:Genital Diseases, Female / Q000473:pathology / Q000628:therapy / Q000821:virology; D006801:Humans; D008403:Mass Screening; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000473:pathology; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000628:therapy / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000628:therapy / Q000821:virology
D004279:DNA, Viral