Biochemical analysis of mouse FKBP60, a novel member of the FKPB family.
We have identified mouse and human FKBP60, a new member of the FKBP gene family. FKBP60 shares strongest homology with FKBP65 and SMAP. FKBP60 contains a hydrophobic signal peptide at the N-terminus, 4 peptidyl-prolyl cis/trans isomerase (PPIase) domains and an endoplasmic reticulum retention motif (HDEL) at the C-terminus. Immunodetection of HA-tagged FKBP60 in NIH-3T3 cells suggests that FKBP60 is segregated to the endoplasmic reticulum. Northern blot analysis shows that FKBP60 is predominantly expressed in heart, skeletal muscle, lung, liver and kidney. With N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as a substrate, recombinant GST-FKBP60 is shown to accelerate effectively the isomerization of the peptidyl-prolyl bond. This isomerization activity is inhibited by FK506. mFKBP60 binds Ca2+ in vitro, presumably by its C-terminal EF-hand Ca2+ binding motif, and is phosphorylated in vivo. hFKBP60 has been mapped to 7p12 and/or 7p14 by fluorescence in situ hybridization (FISH).
Shadidy
M
M
Department of Molecular Genetics, University of Tromsø, Norway.
Caubit
X
X
Olsen
R
R
Seternes
O M
OM
Moens
U
U
Krauss
S
S
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D015870:Gene Expression; D006801:Humans; D020104:Immunophilins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase / Q000037:antagonists & inhibitors / Q000737:chemistry; D010766:Phosphorylation; D021382:Protein Sorting Signals / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D016559:Tacrolimus / Q000494:pharmacology; D022021:Tacrolimus Binding Proteins
D018076:DNA, Complementary; C122078:FKBP9 protein, human; C485685:Fkbp9 protein, mouse; D021382:Protein Sorting Signals; D022021:Tacrolimus Binding Proteins; D020104:Immunophilins; D019696:Peptidylprolyl Isomerase; D002118:Calcium; D016559:Tacrolimus