title: Syntheses and immunologic properties of Escherichia coli O157 O-specific polysaccharide and Shiga toxin 1 B subunit conjugates in mice.
abstract: Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. Mice injected with these bivalent conjugates elicited both bactericidal antibodies to E. coli O157 and neutralization antibodies to Stx1.
authors.affiliation: National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2720, USA.
authors.forename: E
authors.initials: E
authors.lastname: Konadu
authors.forename: A
authors.initials: A
authors.lastname: Donohue-Rolfe
authors.forename: S B
authors.initials: SB
authors.lastname: Calderwood
authors.forename: V
authors.initials: V
authors.lastname: Pozsgay
authors.forename: J
authors.initials: J
authors.lastname: Shiloach
authors.forename: J B
authors.initials: JB
authors.lastname: Robbins
authors.forename: S C
authors.initials: SC
authors.lastname: Szu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D019453:Escherichia coli O157 / Q000276:immunology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D019081:O Antigens / Q000276:immunology*; D022603:Shiga Toxins; D018074:Vaccines, Conjugate / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D019081:O Antigens; D022603:Shiga Toxins; D018074:Vaccines, Conjugate