A controlled release system for proteins based on poly(ether ester) block-copolymers: polymer network characterization.
The properties of a series of multiblock copolymers, based on hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(butylene terephthalate) (PBT) blocks were investigated with respect to their application as a matrix for controlled release of proteins. The degree of swelling, Q, of the copolymers increased with increasing PEG content and with increasing molecular weight of the PEG segment. Within the composition range tested, Q varied from 1.26 for polymers with PEG segments of 600 g/mol and a PBT content of 60 weight.% up to 3.64 for polymers with PEG segments of 4000 g/mol and a PEG/PBT weight ratio of 80:20. Equilibrium stress (compression)-strain measurements were performed in order to estimate mesh sizes. The mesh size of the copolymers ranged from 38 to 93 A, which was experimentally confirmed by diffusion of vitamin B(12) (hydrodynamic diameter d(h)=16.6 A), lysozyme (d(h)=41 A) and bovine serum albumin (d(h)=72 A). The in vitro degradation of PEG/PBT copolymers with a PEG block length of 1000 g/mol and PEG/PBT weight ratios of 70:30, 60:40 and 40:60 was studied. Matrices with increasing PEG contents exhibited a faster weight loss in phosphate-buffered saline (pH 7.4) at 37 degrees C. Over a degradation period of 54 days, M(n) decreased by about 35-45%, while the composition of the matrices, determined by NMR, remained almost constant.
Bezemer
J M
JM
Institute for Biomedical Technology (BMTI), Polymer Chemistry and Biomaterials, Faculty of Chemical Engineering, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.
Grijpma
D W
DW
Dijkstra
P J
PJ
van Blitterswijk
C A
CA
Feijen
J
J
D000818:Animals; D002417:Cattle; D003692:Delayed-Action Preparations / Q000493:pharmacokinetics*; D003993:Dibutyl Phthalate / Q000737:chemistry*; D004355:Drug Stability; D019855:Ethylene Glycol / Q000737:chemistry*; D066298:In Vitro Techniques; D010539:Permeability; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000493:pharmacokinetics*; D012709:Serum Albumin / Q000493:pharmacokinetics; D014805:Vitamin B 12 / Q000493:pharmacokinetics
D003692:Delayed-Action Preparations; D011108:Polymers; D011506:Proteins; D012709:Serum Albumin; D003993:Dibutyl Phthalate; D019855:Ethylene Glycol; D014805:Vitamin B 12