title: Synthesis, characterization and the effect of ligand planarity of [Ru(bpy)2L]2+ on DNA binding affinity.
abstract: Two structurally related ligands (L) 4,5,9,18-tetraazaphenanthreno[9,10-b] triphenylene (taptp) and 2,3-diphenyl-1,4,8,9-tetraazatriphenylene (dptatp), and their related complexes of [Ru(bpy)2L]2+ have been synthesized and characterized by elemental analyses, 1H NMR and mass spectra. Their electrochemical properties were also examined. Both complexes emit intense luminescence in organic solvent but are quenched in water to different extents. The interactions of the complexes with calf thymus DNA have been investigated by viscosity, absorption, emission and circular dichroism spectra. The intrinsic binding constants of [Ru(bpy)2(taptp)]2+ and [Ru(bpy)2(dptatp)]2+ are 1.7 x 10(5) and 3.8 x 10(4) M-1, respectively. All data indicate that both complexes bind enantioselectively to double-stranded calf thymus DNA via the intercalative mode, with stronger affinity for the fully planar ligand complex of [Ru(bpy)2(taptp)]2+.
authors.affiliation: Department of Chemistry, Zhongshan University, Guangzhou, P.R. China.
authors.forename: Q X
authors.initials: QX
authors.lastname: Zhen
authors.forename: B H
authors.initials: BH
authors.lastname: Ye
authors.forename: Q L
authors.initials: QL
authors.lastname: Zhang
authors.forename: J G
authors.initials: JG
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: L N
authors.initials: LN
authors.lastname: Ji
authors.forename: L
authors.initials: L
authors.lastname: Wang
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D066298:In Vitro Techniques; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D015335:Molecular Probes; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry* / Q000378:metabolism*; D013053:Spectrophotometry; D014783:Viscosity
chemical_list: D007364:Intercalating Agents; D008024:Ligands; D015335:Molecular Probes; D009942:Organometallic Compounds; C103599:bis(bipyridyl)ruthenium(II); D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA