Induction of cytotoxic T-cell responses following oral immunization with synthetic peptides encapsulated in PLG microparticles.
CTL responses play a critical role in clearing viral infections. We have investigated the potential of poly(lactide-co-glycolide) (PLG) microparticles as an oral delivery system for peptides representing CTL epitopes from measles virus nucleoprotein. Oral administration of CTL epitopes encapsulated in 50:50 PLG microparticles, resulted in vivo priming of splenic peptide-specific CTL responses. However, the observed CTL lysis was low and cofeeding of encapsulated peptide with cholera toxin as a mucosal adjuvant did not result in any significant enhancement of the observed CTL responses. The pronounced immunostimulatory effect of microparticles, combined with their excellent tissue compatibility and biodegradability makes them a valuable delivery system for synthetic peptide immunogens. However, further work is needed to improve their efficiency via the oral route.
Partidos
C D
CD
Department of Pathology and Infectious Diseases, The Royal Veterinary College, Royal College Street, London, UK.
Vohra
P
P
Jones
D H
DH
Farrar
G
G
Steward
M W
MW
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D002214:Capsules; D002772:Cholera Toxin / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D000939:Epitopes; D005260:Female; D007114:Immunization / Q000379:methods*; D008459:Measles virus / Q000737:chemistry; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000493:pharmacokinetics*; D010455:Peptides / Q000138:chemical synthesis; D011098:Polyglactin 910 / Q000737:chemistry*; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D002214:Capsules; D000939:Epitopes; D009698:Nucleoproteins; D010455:Peptides; D011098:Polyglactin 910; D002772:Cholera Toxin