Fractionation of apple procyanidins by size-exclusion chromatography.
Oligomeric constituents of apple procyanidins were fractionated by size-exclusion chromatography using a TSKgel Toyopearl HW-40F column. The best separation was obtained using a mobile phase of acetone-8 M urea (6:4; adjusted to pH 2) at a flow-rate of 1.0 ml/min. In this chromatographic system, the use of 8 M urea in the mobile phase resulted in a molecular sieve effect without any surface affinity interaction between the gel beads and the procyanidin molecules. Each fraction obtained was examined by reversed-phase high-performance liquid chromatography and time-of-flight mass spectrometry. The order of elution of the procyanidins from the column was coincident with their degree of polymerization.
Yanagida
A
A
Institute for Production Research and Development, Nikka Whisky Distilling Co. Ltd., Chiba, Japan.
Kanda
T
T
Shoji
T
T
OhnishiKameyama
M
M
Nagata
T
T
D044946:Biflavonoids; D002392:Catechin / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D044945:Proanthocyanidins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
D044946:Biflavonoids; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin