title: Characterization of the Coxiella burnetti sucB gene encoding an immunogenic dihydrolipoamide succinyltransferase.
abstract: The Coxiella burnetii sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme was cloned by immunological screening of a lambda EMBL3 genomic library prepared from strain Nine Mile DNA and sequenced. The homology of the cloned gene product to the counterpart in Escherichia coli was 54.3%, but the homology of the N-terminal region was only 42%. The gene was expressed in E. coli as an independent unit from its own promoter, producing an immunoreactive protein of about 50 kDa on SDS-PAGE which reacted with antisera from laboratory animals and sera from human patients with acute Q fever. The study results suggest that the C. burnetii E2o enzyme may serve as a potential target antigen for diagnostic assays for Q fever.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
authors.forename: S V
authors.initials: SV
authors.lastname: Nguyen
authors.forename: H
authors.initials: H
authors.lastname: To
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D016997:Coxiella burnetii / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011778:Q Fever / Q000097:blood / Q000276:immunology; D011817:Rabbits; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D000217:Acyltransferases; C021515:dihydrolipoamide succinyltransferase