Endothelin-1 effect on tyrosine phosphorylation and on tyrosine phosphatase (PTP-1C) translocation in rabbit platelets.
This study examined the temporal relationships of endothelin-1-stimulated rabbit platelets tyrosine phosphorylated proteins. The effect of endothelin-1 on tyrosine phosphorylation was dose- and time-dependent and caused a rapid tyrosine phosphorylation of three groups of proteins in the molecular mass range 70-100 kDa, 100-150 kDa and 150-200 kDa. Significant protein tyrosine phosphatase activity and amount were found to be associated with the cytoskeleton of endothelin-1-stimulated rabbit platelets. Under our experimental conditions, translocation from the cytosolic fraction to the cytoskeleton reached its highest levels within 10-20 sec of endothelin-1 stimulation. Endothelin-1-induced translocation of protein tyrosine phosphatase, associated with the increase in its activity was demonstrated by immunoblotting and immunoelectron microscopy.
Catalán
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RE
Departamento Biología Molecular/Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM) Universidad Autónoma de Madrid, Spain.
Gargiulo
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L
Martínez
A M
AM
Liras
A
A
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology*; D010766:Phosphorylation; D015539:Platelet Activation / Q000187:drug effects; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011817:Rabbits; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism
D019332:Endothelin-1; D014443:Tyrosine; D017027:Protein Tyrosine Phosphatases