title: [Mutation analysis of p16 gene in non-small cell lung carcinomas].
abstract: OBJECTIVE
  To investigate the role of p16 gene in the tumorigenesis of non-small cell lung carcinomas (NSCLC).
  
  
  METHODS
  Homozygous deletion and mutation of exon 2 of p16 genes in 40 NSCLC tissues were analyzed using PCR and dsDNA direct sequencing technique.
  
  
  RESULTS
  In 2 NSCLC tissues, homozygous deletions of p16 gene were detected; in 14 NSCLC tissues, 19 point mutations and a frameshift were detected.
  
  
  CONCLUSIONS
  Point mutations of p16 gene were frequent and might play a role in the initiation and progression of NSCLC. Nt380 in this gene was a hot spot of mutation.
authors.affiliation: Department of Medical Genetics, Harbin Medical University, Harbin, Henongjiang, 150086 P. R. China. fusb@ems.hrbmu.edu.cn
authors.forename: S
authors.initials: S
authors.lastname: Fu
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Li
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D019942:Genes, p16; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation