Identification and confirmation of 3-hydroxy metabolite of ketoprofen in camels by gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy.
The metabolites of ketoprofen were investigated in five camels following intravenous administration of a dose of 2.0 mg/kg body weight. Two metabolites were identified. The first one was purified with thin-layer chromatography. It was identified by gas chromatography-mass spectrometry (GC-MS) in comparison with authenticated reference standard and was found to be hydroxyketoprofen due to reduction of the ketone group of ketoprofen. The second metabolite was purified by high-performance liquid chromatography. It was identified with GC-MS and nuclear magnetic resonance spectroscopy as 3-hydroxybenzolketoprofen resulting from oxidation of the aromatic ring.
Alkatheeri
N A
NA
Camelracing Forensic Laboratory, Forensic Science Laboratory, Abu Dhabi, United Arab Emirates.
Wasfi
I A
IA
Kvanagh
P
P
Lambert
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000378:metabolism*; D002162:Camelus; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007660:Ketoprofen / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007660:Ketoprofen