title: Preparation of highly purified momordin II without ribonuclease activity.
abstract: Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.
authors.affiliation: Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Valbonesi
authors.forename: L
authors.initials: L
authors.lastname: Barbieri
authors.forename: A
authors.initials: A
authors.lastname: Bolognesi
authors.forename: E
authors.initials: E
authors.lastname: Bonora
authors.forename: L
authors.initials: L
authors.lastname: Polito
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
mesh_terms: D000225:Adenine / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D002847:Chromatography, Agarose; D004275:DNA, Ribosomal / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D012260:Ribonucleases / Q000302:isolation & purification*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012503:Saponins / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*
chemical_list: D004275:DNA, Ribosomal; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012503:Saponins; C076925:momordin II (protein); D012260:Ribonucleases; D000225:Adenine