Oncogenic potential of EAG K(+) channels.
We have investigated the possible implication of the cell cycle-regulated K(+) channel ether à go-go (EAG) in cell proliferation and transformation. We show that transfection of EAG into mammalian cells confers a transformed phenotype. In addition, human EAG mRNA is detected in several somatic cancer cell lines, despite being preferentially expressed in brain among normal tissues. Inhibition of EAG expression in several of these cancer cell lines causes a significant reduction of cell proliferation. Moreover, the expression of EAG favours tumour progression when transfected cells are injected into immune-depressed mice. These data provide evidence for the oncogenic potential of EAG.
Pardo
L A
LA
Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Strasse 3, D-37075 Göttingen, Germany. pardo@mail.mpiem.gwdg.de
del Camino
D
D
Sánchez
A
A
Alves
F
F
Brüggemann
A
A
Beckh
S
S
Stühmer
W
W
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D051638:Ether-A-Go-Go Potassium Channels; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D010641:Phenotype; D015221:Potassium Channels / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D051638:Ether-A-Go-Go Potassium Channels; C496884:KCNH1 protein, human; C496885:Kcnh1 protein, mouse; D020319:Oligodeoxyribonucleotides, Antisense; D015221:Potassium Channels; D012333:RNA, Messenger