title: Intra and inter-specific microsatellite variation in the Leishmania subgenus Viannia.
abstract: Leishmania species of the subgenus Viannia are responsible for a large proportion of New World leishmaniasis. Here we report the development of a set of microsatellite markers which are able to discriminate between all species within the subgenus Viannia, including the closely related species pairs: Leishmania (V.) braziliensis and Leishmania (V.) peruviana; Leishmania (V.) panamensis and Leishmania (V.) guyanensis. Potential species hybrids were uncovered in the analysis. These markers are sufficiently polymorphic such that within-species epidemiological, population and genetic studies are theoretically possible for all species analyzed.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Russell
authors.forename: M P
authors.initials: MP
authors.lastname: Iribar
authors.forename: B
authors.initials: B
authors.lastname: Lambson
authors.forename: S
authors.initials: S
authors.lastname: Brewster
authors.forename: J M
authors.initials: JM
authors.lastname: Blackwell
authors.forename: C
authors.initials: C
authors.lastname: Dye
authors.forename: J W
authors.initials: JW
authors.lastname: Ajioka
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007891:Leishmania / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan