The effect of enoxaparin on bleomycin-induced lung injury in mice.
We have evaluated the effect of enoxaparin, a potent antithrombotic drug, on bleomycin (Bleo)-induced pulmonary inflammation in mice. Pulmonary injury was induced by a single intratracheal (i.t.) instillation of Bleo. Four groups of female C57BL/6 mice, each received one of four treatments: (1) i.t. Bleo and daily intraperitoneal (i.p.) injections of enoxaparin (EN) starting one day before i.t. instillation of Bleo (Bleo-EN); (2) i.t. Bleo and i.p. injections of saline (Bleo-Sal); (3) i.t. saline and i.p. enoxaparin (Sal-EN); (4) i.t. saline and i.p. saline (Sal-Sal). Animals were sacrificed 14 days after i.t. treatment. Lung injury was evaluated by analysis of bronchoalveolar lavage fluid and histologically by an overall semiquantitative index of lung injury and a quantitative image analysis assessing alveolar wall area fraction and fibrosis fraction. Treatment of mice with enoxaparin did not ameliorate Bleo-induced lung injury. Our study does not establish a critical role of procoagulant activity in the evolution of Bleo-induced lung injury and does not support the use of antithrombotic therapy for the prevention of pulmonary fibrosis.
Laxer
U
U
Lung Cellular and Molecular Biology Laboratory, Hadassah University Hospital, Jerusalem, Israel.
Lossos
I S
IS
Gillis
S
S
Or
R
R
Christensen
T G
TG
Goldstein
R H
RH
Breuer
R
R
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001761:Bleomycin / Q000633:toxicity; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count / Q000187:drug effects; D017984:Enoxaparin / Q000494:pharmacology*; D015951:Factor Xa / Q000032:analysis; D005260:Female; D007091:Image Processing, Computer-Assisted; D007274:Injections, Intraperitoneal; D008168:Lung / Q000187:drug effects*; D017563:Lung Diseases, Interstitial / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL
D000925:Anticoagulants; D017984:Enoxaparin; D001761:Bleomycin; D015951:Factor Xa