Liquid chromatographic determination of fenbendazole residues in pig tissues after treatment with medicated feed.
Fenbendazole (FBZ) is an anthelmintic widely used in farm animals to treat parasitic infestations. In pigs, it is administered in the food. The aim of this study was to validate an analytical method for the determination of FBZ and its metabolites in pig tissues. This method is based on oxidation of FBZ and its sulfoxide metabolite to the sulfone metabolite (FBZSO2). The limit of quantitation for this method is 20 ng FBZSO2/g for all tissues. The maximum residue limits (MRLs) established for FBZ and its metabolites in pig tissues are 50 ng/g for muscle, fat, and kidney and 500 ng/g for liver. This method is based on a liquid-liquid extraction followed by an oxidation with peracetic acid and a cleanup procedure based on 2 liquid-liquid extractions. Determination is achieved by high-performance liquid chromatography with ultraviolet detection. The present method is adjusted to the MRL established for FBZ and its metabolite residues. The analysis of the residues shows that after 72 h posttreatment, no FBZSO2 was detected in muscle, fat, and kidney and that liver levels were below the MRL.
Capece
B P
BP
Unitat de Farmacologia i de Terapèutica, Facultat de Veterinària, UAB, Bellaterra, Spain.
Pérez
B
B
Castells
E
E
Arboix
M
M
Cristòfol
C
C
D000273:Adipose Tissue / Q000737:chemistry; D000821:Animal Feed; D000818:Animals; D000969:Antinematodal Agents / Q000032:analysis*; D002138:Calibration; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D005273:Fenbendazole / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D016014:Linear Models; D008099:Liver / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
D000969:Antinematodal Agents; D005273:Fenbendazole