Visualizing "greengold" clusters in the STEM.
"Greengold" is a large metallic cluster thought to contain 75 gold atoms in a compact 1.4-nm-diameter core surrounded by an organic shell. Scanning transmission electron microscope imaging shows uniform mass and size distributions with an apparent mass of 24 kDa, unaffected by radiation damage. The signal-to-noise ratio is adequate for visualization at low dose and in the presence of a relatively thick biological matrix. Under some conditions these clusters have a slight tendency to form linear chains and 2-D hexagonal arrays with a spacing of 2.6 nm. The parameters presented permit estimation of the feasibility of proposed labeling experiments.
Wall
J S
JS
Biology Department, Brookhaven National Laboratory, Building 463, Upton, New York 11973, USA.
D003116:Color; D004307:Dose-Response Relationship, Radiation; D017612:Gold Compounds / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000528:radiation effects*; D012815:Signal Processing, Computer-Assisted; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000528:radiation effects
D017612:Gold Compounds; D009942:Organometallic Compounds