title: Heterogeneity in expression of cytokine mRNA in freshly isolated peripheral blood eosinophils of patients with cutaneous-disease-associated eosinophilia.
abstract: BACKGROUND
  It is thought that there are subpopulations of eosinophils concerning cytokine production: some of them predominantly produce Th1 cytokines, while others produce Th2.
  
  
  METHODS
  By semiquantitative RT-PCR, the in vivo levels of the cytokine mRNA were measured in freshly isolated eosinophils and peripheral blood mononuclear cells (PBMCs) of 19 patients with eosinophilia, including 6 with atopic dermatitis, 4 with drug eruption, 1 with chronic dermatitis, 2 with erythroderma, 3 with bullous pemphigoid, 1 each with urticaria, adult T cell lymphoma, and hypereosinophilic syndrome, and 7 healthy individuals. Eosinophils and PBMCs were isolated by centrifugation over Ficoll-Paque and magnetic cell separation using anti-CD16 antibody.
  
  
  RESULTS
  The IL-4/IFN-gamma ratio in eosinophils as well as PBMCs was increased and correlated well with IgE levels in atopic dermatitis. In other cytokines, such as IL-8, TNF-alpha and TGF-beta1, the levels of mRNA expression were variable among each sample.
  
  
  CONCLUSIONS
  Not only helper T lymphocytes but also eosinophils may take part in the pathogenesis of cutaneous diseases by virtue of heterogeneity of Th1 and Th2 cytokine production.
authors.affiliation: Department of Dermatology, Oita Medical University, Oita, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hatano
authors.forename: K
authors.initials: K
authors.lastname: Katagiri
authors.forename: S
authors.initials: S
authors.lastname: Takayasu
mesh_terms: D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D004802:Eosinophilia / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012871:Skin Diseases / Q000150:complications / Q000276:immunology*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger