HLA class I and II typing of the patients with Behçet's disease in Saudi Arabia.
Thirteen Saudi Arabian patients with Behçet's disease (BD) were typed for HLA-A and -B alleles by the conventional serologic typing and for HLA-DRB1, -DQB1 and -DPB1 alleles by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. As a result, the phenotype frequency of the B51 antigen was significantly increased in the patient group as compared to the ethnically matched control group (76.9% in patients vs. 22.20% in controls), but no significant difference was observed in HLA-A, -DRB1, -DQB1 or -DPB1 alleles between the patients and controls, as previously observed in Japanese BD. Further, by HLA-B51 allelic genotyping performed by the polymerase chain reaction-sequence specific primers (PCR-SSP) method, all of the B51-positive patients and controls were found to carry one particular allele, B*5101, except one patient with B*5108.
Yabuki
K
K
Department of Genetic Information, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Ohno
S
S
Mizuki
N
N
Ando
H
H
Tabbara
K F
KF
Goto
K
K
Nomura
E
E
Nakamura
S
S
Ito
N
N
Ota
M
M
Katsuyama
Y
Y
Inoko
H
H
D001528:Behcet Syndrome / Q000235:genetics*; D004247:DNA / Q000097:blood; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012529:Saudi Arabia
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D004247:DNA