Preparation of highly purified momordin II without ribonuclease activity.
Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.
Valbonesi
P
P
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Barbieri
L
L
Bolognesi
A
A
Bonora
E
E
Polito
L
L
Stirpe
F
F
D000225:Adenine / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D002847:Chromatography, Agarose; D004275:DNA, Ribosomal / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D012260:Ribonucleases / Q000302:isolation & purification*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012503:Saponins / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*
D004275:DNA, Ribosomal; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012503:Saponins; C076925:momordin II (protein); D012260:Ribonucleases; D000225:Adenine