Alpha-synuclein immunoreactive Lewy bodies and Lewy neurites in Parkinson's disease are detectable by an advanced silver-staining technique.
Immunostaining with anti-alpha-synuclein is used to detect Lewy bodies and Lewy neurites in cases of Parkinson's disease and related disorders. To prove that the result of a modern silver method is equivalent to that achieved with immunoreactions for alpha-synuclein, individual sections were successively processed using both methods. The silver-stained sections showed all of the immunoreactive Lewy bodies, and thin Lewy neurites were detected equally well by both techniques. The present study, therefore, points to the capabilities of a modern silver-staining method which is less time consuming and less expensive than immunocytochemical techniques.
Sandmann-Keil
D
D
Department of Anatomy, J. W. Goethe University, Theodor Stern Kai 7, D-60590 Frankfurt/Main, Germany.
Braak
H
H
Okochi
M
M
Haass
C
C
Braak
E
E
D006801:Humans; D016631:Lewy Bodies / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010300:Parkinson Disease / Q000473:pathology*; D016622:Silver Staining / Q000379:methods*; D051843:Synucleins; D051844:alpha-Synuclein
D009419:Nerve Tissue Proteins; C497604:SNCA protein, human; D051843:Synucleins; D051844:alpha-Synuclein