Molecular cloning and expression of human neurochondrin-1 and -2.
Human neurochondrins have been cloned from a brain cDNA library. The human neurochondrin-1 and -2 predict leucine-rich (15.8 and 15.9%) proteins of 729 and 712 amino acid residues, with molecular weights of 78.9 and 77.2 kDa, respectively. The deduced amino acid sequence indicates 98% identity among human, mouse and rat species. Northern analysis indicates that about 4 kb human neurochondrin mRNAs are abundant in the fetal and the adult brain.
Mochizuki
R
R
Sumitomo Pharmaceuticals Research Center, Sumitomo Pharmaceuticals, Osaka, Japan.
Ishizuka
Y
Y
Yanai
K
K
Koga
Y
Y
Fukamizu
A
A
Murakami
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment
D018076:DNA, Complementary; C122108:NCDN protein, human; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C122109:neurochondrin-2