The expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia-reperfusion injury.
We evaluated the proinflammatory cytokines, TNF-alpha and IL-1beta, mRNA expression in the rat sciatic and tibial nerves following ischemia-reperfusion (IR) injury, using competitive RT-PCR, to explore the role of cytokines in IR injury. The expressions of both TNF-alpha and IL-1beta mRNA were related to severity of ischemia and occurred with reperfusion rather than ischemia alone. TNF-alpha gene expression peaked at 24 h of reperfusion, while that of IL-1beta peaked at 12 h. These data support the notion that the proinflammatory cytokines TNF-alpha and IL-1beta are involved in the inflammatory response of IR injury to the peripheral nervous system and may be involved in the pathophysiology of ischemic fiber degeneration.
Mitsui
Y
Y
Department of Neurology, Mayo Clinic, 811 Guggenheim Bldg., 200 First Street SW, Rochester, MN 55905, USA.
Okamoto
K
K
Martin
D P
DP
Schmelzer
J D
JD
Low
P A
PA
D000818:Animals; D018076:DNA, Complementary; D015870:Gene Expression / Q000502:physiology; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*; D013979:Tibial Nerve / Q000098:blood supply* / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D018076:DNA, Complementary; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha