title: Physiological changes of Salmonella typhimurium cells under osmotic and starvation conditions by image analysis.
abstract: The effects of starvation and salinity on the physiology of Salmonella typhimurium were investigated in a microcosm study. The physiological changes were monitored by using fluorochromes dyes such as DAPI (4',6-diamidino-2-phenylindole) for evaluation of the genomic content, CTC (5-cyano-2,3-ditolyl tetrazolium chloride) for respiratory activity and syto 9 and propidium iodide for cytoplasmic membrane damages. The metabolic activity of the cellular population was assessed with the method of Kogure (direct viable count), to enumerate the substrate-responsive cells. These different staining procedures were objectively analysed by an image analysis system. This paper describes the progressive alteration of Salmonella typhimurium physiology under salinity and starvation conditions.
authors.affiliation: Laboratoire Hydrobiologie Marine et Continentale, UMR CNRS-Université Montpellier II No. 5556, Université de Montpellier II, F-34095, Montpellier, France.
authors.forename: A
authors.initials: A
authors.lastname: Caro
authors.forename: P
authors.initials: P
authors.lastname: Got
authors.forename: B
authors.initials: B
authors.lastname: Baleux
mesh_terms: D008856:Microscopy, Fluorescence; D009997:Osmotic Pressure; D012486:Salmonella typhimurium / Q000502:physiology*