Translational regulation of oskar mRNA occurs independent of the cap and poly(A) tail in Drosophila ovarian extracts.
Translational regulation plays a prominent role in Drosophila body patterning. Progress in elucidating the underlying mechanisms has been limited by the lack of a homologous in vitro system that supports regulation. Here we show that extracts prepared from Drosophila tissues are competent for translation. Ovarian extracts, but not embryonic extracts, support the Bruno response element- and Bruno-dependent repression of oskar mRNA translation, which acts in vivo to prevent protein synthesis from transcripts not localized to the posterior pole of the oocyte. Consistent with suggestive evidence from in vivo experiments, regulation in vitro does not involve changes in poly(A) tail length. Moreover, inhibition studies strongly suggest that repression does not interfere with the process of 5' cap recognition. Translational regulation mediated through the Bruno response elements is thus likely to occur via a novel mechanism.
Lie
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Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.
Macdonald
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PM
D000818:Animals; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics*; D010053:Ovary / Q000502:physiology; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000502:physiology
D029721:Drosophila Proteins; D019476:Insect Proteins; D012315:RNA Caps; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C093437:bru1 protein, Drosophila; C091675:osk protein, Drosophila