title: Isolation, cloning, and expression of a new murine zinc finger encoding gene.
abstract: With the aim of identifying genes involved in cartilage differentiation, we have used a subtractive hybridization strategy with cDNAs from a chondrocytic cell line (MC615) and mRNAs from a mesenchymal precursor cell line (10T1/2). We have isolated a cDNA clone representing a novel mouse gene. The predicted 368-amino acid protein, designated ZF-12, contains four C(2)H(2)-type zinc finger motifs and one region homologous to the LeR domain, a finger-associated structural domain. ZF-12 mRNAs are expressed during embryonic development and in different organs in adult, including rib cartilage. These data suggest that ZF-12 might play an important role not only in cartilage differentiation, but also in basic cellular processes.
authors.affiliation: Institut de Biologie et Chimie des Protéines, CNRS UPR 412, 7 passage du Vercors 69367, Lyon, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Prost
authors.forename: D
authors.initials: D
authors.lastname: Nègre
authors.forename: F
authors.initials: F
authors.lastname: Cornet-Javaux
authors.forename: J C
authors.initials: JC
authors.lastname: Cortay
authors.forename: A J
authors.initials: AJ
authors.lastname: Cozzone
authors.forename: D
authors.initials: D
authors.lastname: Herbage
authors.forename: F
authors.initials: F
authors.lastname: Mallein-Gerin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D016335:Zinc Fingers / Q000235:genetics*