The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes.
The domestic dog (Canis familiaris) is widely used as a model in the study of human disease. However, many of the 78 chromosomes comprising the canine karyotype are extremely difficult to identify reliably by classical cytogenetics. This has been a major hindrance to molecular cytogenetic studies of this species. The Animal Health Trust and the Sanger Centre have developed a set of canine whole chromosome-specific fluorescence in situ hybridisation (FISH) probes (chromosome paints). We have used these chromosome paints to identify unequivocally each chromosome in a metaphase spread. An increasing number of laboratories are making use of cooled CCD cameras and sophisticated software for FISH mapping. Consequently, there is a major trend towards the use of DAPI banding for concurrent chromosome identification during FISH analyses in a range of species. Here we present, for the first time, a complete DAPI banded karyotype of the dog in which each chromosome has been accurately placed, together with a 460-band DAPI ideogram. These data will facilitate the accurate assignment of FISH-mapped loci to all chromosomes comprising the karyotype and form the basis for an agreed standard of the dog karyotype.
Breen
M
M
Animal Health Trust, Kentford, Suffolk, UK. mtbreen@hgmp.mrc.ac.uk
Bullerdiek
J
J
Langford
C F
CF
D000818:Animals; D002871:Chromosome Banding; D020223:Chromosome Painting; D002875:Chromosomes; D004285:Dogs / Q000235:genetics*; D005456:Fluorescent Dyes; D007211:Indoles; D007621:Karyotyping; D008214:Lymphocytes
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI