Location and catalytic characteristics of a multipotent bacterial polyphenol oxidase.
The melanogenic marine bacterium Marinomonas mediterranea contains a multipotent polyphenol oxidase (PPO) able to oxidize substrates characteristic for tyrosinase and laccase. Thus, this enzyme shows tyrosine hydroxylase activity and it catalyzes the oxidation of a wide variety of o-diphenol as well as o-methoxy-activated phenols. The study of its sensitivity to different inhibitors also revealed intermediate features between laccase and tyrosinase. It is similar to tyrosinases in its sensitivity to tropolone, but it resembles laccases in its resistance to cinnamic acid and phenylthiourea, and in its sensitivity to fluoride anion. This enzyme is mostly membrane-bound and can be solubilized either by non-ionic detergent or lipase treatments of the membrane. The expression of this enzymatic activity is growth-phase regulated, reaching a maximum in the stationary phase of bacterial growth, but L-tyrosine, Cu(II) ions, or 2,5-xylidine do not induce it. This enzyme can be separated from a second PPO form by gel permeation chromatography. The second PPO is located in the soluble fraction and shows a sodium dodecyl sulfate (SDS)-activated action on the characteristic substrates for tyrosinase, L-tyrosine, and L-dopa, but it does not show activity towards laccase-specific substrates. The involvement of the multipotent PPO in melanogenesis and its relationship with the SDS-activated form and with the alternative functions proposed for multicopper oxidases in other microorganisms are discussed.
Fernández
E
E
Department of Biochemistry and Molecular Biology B, University of Murcia, Spain.
Sanchez-Amat
A
A
Solano
F
F
D002384:Catalysis; D004156:Catechol Oxidase / Q000737:chemistry / Q000378:metabolism*; D002845:Chromatography; D003300:Copper / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D042845:Laccase; D008543:Melanins / Q000378:metabolism*; D008544:Melanocytes / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D013379:Substrate Specificity; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D008543:Melanins; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D003300:Copper; D010088:Oxidoreductases; D004156:Catechol Oxidase; D042845:Laccase; D014446:Tyrosine 3-Monooxygenase; D014442:Monophenol Monooxygenase