Study on the bioavailability of baicalin-phospholipid complex by using HPLC.
A complex of baicalin with soy phospholipid was prepared to improve the bioabsorption of baicalin. A simple and sensitive HPLC method was developed for baicalin determination in rat plasma. It was shown that its plasma concentration reached a peak of 0.42 microgram/mL 5.3 h after oral administration, 600 mg/kg. However, after intake of its phospholipid complex, a peak of 0.90-microgram/mL occurred at a later time, 6.1 h. The elimination of the complex tended to be slower than that of the free drug. There was a significant difference in the mean area under the concentration-time curves (AUC) between the free drug and the complex (p < 0.01).
Wu
J
J
Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110015, People's Republic of China.
Chen
D
D
Zhang
R
R
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000493:pharmacokinetics*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004337:Drug Carriers; D005419:Flavonoids / Q000493:pharmacokinetics*; D008297:Male; D000432:Methanol / Q000737:chemistry; D009579:Nitrobenzoates / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results
D000893:Anti-Inflammatory Agents; D004337:Drug Carriers; D005419:Flavonoids; D009579:Nitrobenzoates; D010743:Phospholipids; C038044:baicalin; C026017:3,5-dinitrobenzoic acid; D000432:Methanol