title: The rough deal protein is a new kinetochore component required for accurate chromosome segregation in Drosophila.
abstract: Mutations in the rough deal (rod) gene of Drosophila greatly increase the missegregation of sister chromatids during mitosis, suggesting a role for this gene product in spindle or kinetochore function. The activity provided by rod also appears to be necessary for the recruitment of two known kinetochore components, Zw10 and cytoplasmic dynein. In this paper we describe the cloning of rough deal and an initial cytological characterization of its product. The Rod protein shares no identifiable structural motif with other known proteins, although apparent homologs exist in the genomes of nematode and man. By immunocytochemistry we show that Rod displays a dynamic intracellular staining pattern, localizing first to kinetochores in prometaphase, but moving to kinetochore microtubules at metaphase. Early in anaphase the protein is once again restricted to the kinetochores, where it persists until the end of telophase. This behavior is in all respects similar to that described for Zw10, and suggests that the proteins function together.
authors.affiliation: C.N.R.S., Centre de Génétique Moléculaire, Avenue de la Terrasse, France.
authors.forename: F
authors.initials: F
authors.lastname: Scaërou
authors.forename: I
authors.initials: I
authors.lastname: Aguilera
authors.forename: R
authors.initials: R
authors.lastname: Saunders
authors.forename: N
authors.initials: N
authors.lastname: Kane
authors.forename: L
authors.initials: L
authors.lastname: Blottière
authors.forename: R
authors.initials: R
authors.lastname: Karess
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects; D018797:Cell Cycle Proteins; D020090:Chromosome Segregation / Q000502:physiology*; D003078:Colchicine / Q000494:pharmacology; D003360:Cosmids / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D015151:Immunoblotting; D007150:Immunohistochemistry; D018386:Kinetochores / Q000737:chemistry*; D007814:Larva / Q000235:genetics*; D008297:Male; D008540:Meiosis / Q000502:physiology; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology; D013090:Spermatocytes / Q000737:chemistry; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; C064993:ROD protein, Drosophila; D014157:Transcription Factors; D017239:Paclitaxel; D003078:Colchicine