title: The structure of the core part of Proteus mirabilis O27 lipopolysaccharide with a new type of glycosidic linkage.
abstract: The structural assignment of the intact lipopolysaccharide core from Proteus mirabilis O27 has been completed based on a combination of chemical degradation studies, NMR spectroscopy and ES-MS spectroscopy. The overall core structure is as follows: [formula: see text] where all sugars are in the pyranose form except the N-acetylglycosamine residue, Hep refers to L-glycero-alpha-D-manno-heptopyranose and alpha-DDHep to D-glycero-alpha-D-manno-heptopyranose. Bold italics indicate non-stoichiometric substituents. A new type of glycosidic linkage has been discovered wherein a GalNAc residue is linked as an open form acetal to the 4- and 6-positions of a 2-amino-2-deoxygalactopyranose residue. This structural element is abbreviated GaloNAc-4,6-, where the 'o' indicates the open form of the sugar residue.
authors.affiliation: Carlsberg Laboratory, Department of Chemistry, Copenhagen, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Vinogradov
authors.forename: K
authors.initials: K
authors.lastname: Bock
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006027:Glycosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry*
chemical_list: D006027:Glycosides; D019081:O Antigens