title: Lectin activity of Bacillus thuringiensis parasporal inclusion proteins.
abstract: Parasporal inclusion proteins from a total of 151 Bacillus thuringiensis strains, consisting of 139 Japanese isolates and the type strains of 12 H serovars, were screened for haemagglutination (HA) activity against sheep erythrocytes. Of 58 B. thuringiensis strains with HA activity, nine strains exhibited high activity and the remaining 49 strains were moderately active. The strains with high HA activity were derived from phylloplanes and soils of five geographically different localities, and belonged to H serovars kurstaki and other undefined serotype(s). The HA activities in the four selected strains were generated only when alkali-solubilised parasporal inclusion proteins were proteolytically processed. Furthermore, the lectin activity of the four strains was strongly inhibited by preincubation with N-acetylgalactosamine. The lectin-producing B. thuringiensis strains were heterogeneous in other biological activities of parasporal inclusions: insecticidal activity and cytocidal action on human leukaemia T cells.
authors.affiliation: Biotechnology and Food Research Institute, Fukuoka Industrial Technology Centre, 1465-5 Aikawa, Kurume, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akao
authors.forename: E
authors.initials: E
authors.lastname: Mizuki
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: H
authors.initials: H
authors.lastname: Saitoh
authors.forename: M
authors.initials: M
authors.lastname: Ohba
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis* / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007306:Insecticides / Q000494:pharmacology*; D037102:Lectins / Q000032:analysis* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D007306:Insecticides; D037102:Lectins