[Study of leukocyte phagocytic activity using flow cytometry and hydrophilic fluorochrome-labeled microspheric particles].
The phagocytic activity was determined in human polymorphonuclear leucocytes using the flow cytometry technique with microspheric hydrophilic particles labelled with fluorescein isothiocyanate. Non-specific adherence of particles is discussed and normal values of phagocytic activity are presented. The advantages of this method include simplified sample preparation using whole blood, rapidity of the test, precise phagocytosis quantification and possibility of archiving all findings in a computer.
Malícková
K
K
Ustav klinické imunologie a alergologie, Vseobecná fakultní nemocnice a 1. lékarská fakulta Univerzity Karlovy, Praha.
Sedláková
Z
Z
Rerichová
M
M
Marecková
H
H
Fucíková
T
T
D000328:Adult; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis
D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate