A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis.
We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.
Nishiyama
Y
Y
Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto, Japan.
Hirota
T
T
Morisaki
T
T
Hara
T
T
Marumoto
T
T
Iida
S
S
Makino
K
K
Yamamoto
H
H
Hiraoka
T
T
Kitamura
N
N
Saya
H
H
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000378:metabolism*
D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D011494:Protein Kinases; C092745:LATS1 protein, human; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases