Glucose regulation of the expression of the glucagon receptor gene.
The glucagon receptor gene is a member of a gene family, the expression of which is strongly upregulated by glucose. This review deals with the structure of both the glucagon receptor gene and its promoter. Attention is focused on the glucose regulatory element that we discovered in the promoter of this gene. Regulation by glucose of genes implicated in glucose homeostasis represents one mechanism contributing to the control of fuel utilization. Its deficiency or imbalance could potentially lead to or participate in pathological situations such as diabetes mellitus. On the other hand, the regulatory element of the glucagon receptor gene promoter could be used as a tool for the glucose-regulated expression of other genes. Indeed, an analysis of the glucagon receptor gene promoter demonstrated that only a short fragment of the genomic DNA, easy to subclone, contains all required elements for activation by glucose. Its potential use for gene therapy is also considered, therefore, in this report.
Svoboda
M
M
Laboratory of Biochemistry and Nutrition, Université Libre de Bruxelles, Brussels, B-1070, Belgium. msvobod@.ulb.ac.be
Portois
L
L
Malaisse
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018027:Receptors, Glucagon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018027:Receptors, Glucagon; D005947:Glucose