Can the fatty acid selectivity of plant lipases be predicted from the composition of the seed triglyceride?
To address the question can the fatty acid selectivity of plant lipases be predicted from the composition of the seed triglyceride, we have characterised the selectivity of lipases from a wide range of oilseeds with diverse fatty acid compositions. For this study, a novel hydrolysis assay using a fully randomised oil, was developed. From some seed sources (e.g. Cinnamomum camphora), lipases show high preference for particular fatty acids, whilst from others (e.g. Brassica napus, Theobroma cacao80% saturated or 'unusual' fatty acids may contain lipases which exhibit selectivity. It therefore follows that since the majority of seeds are composed of unsaturated fatty acids, that highly selective lipases will be unusual in nature. However lipases from some species of the Cuphea genera show exceptionally high preference for particular fatty acids. For example, lipase from seeds of Cuphea procumbans has over 20-fold selectivity for C10:0.
Hellyer
S A
SA
Unilever Research, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK. amanda.hellyer@unilever.com
Chandler
I C
IC
Bosley
J A
JA
D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008049:Lipase / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D010944:Plants / Q000201:enzymology*; D012639:Seeds / Q000737:chemistry / Q000201:enzymology*; D013379:Substrate Specificity; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010936:Plant Extracts; D010938:Plant Oils; D014280:Triglycerides; D008049:Lipase