A defect in beta-oxidation causes abnormal inflorescence development in Arabidopsis.
The abnormal inflorescence meristem1 (aim1) mutation affects inflorescence and floral development in Arabidopsis. After the transition to reproductive growth, the aim1 inflorescence meristem becomes disorganized, producing abnormal floral meristems and resulting in plants with severely reduced fertility. The derived amino acid sequence of AIM1 shows extensive similarity to the cucumber multifunctional protein involved in beta-oxidation of fatty acids, which possesses l-3-hydroxyacyl-CoA hydrolyase, l-3-hydroxyacyl-dehydrogenase, d-3-hydroxyacyl-CoA epimerase, and Delta(3), Delta(2)-enoyl-CoA isomerase activities. A defect in beta-oxidation has been confirmed by demonstrating the resistance of the aim1 mutant to 2,4-diphenoxybutyric acid, which is converted to the herbicide 2,4-D by the beta-oxidation pathway. In addition, the loss of AIM1 alters the fatty acid composition of the mature adult plant.
Richmond
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TA
Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Bleecker
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D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D018525:Germination; D006540:Herbicides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid
C401414:AIM1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D005227:Fatty Acids; D006540:Herbicides; D009097:Multienzyme Complexes; C036483:T-DNA; D004746:Enoyl-CoA Hydratase