title: Intracellular ATP depletion inhibits swelling-induced D-[3H]aspartate release from primary astrocyte cultures.
abstract: Volume expansion-sensing outward rectifier (VSOR) anion channel, also referred to as volume-sensitive organic osmolyte-anion channel (VSOAC), appears to be responsible for cell swelling-induced amino acid release in a variety of cells. One prominent feature of the VSOR/VSOAC is that non-hydrolyzed intracellular ATP binding to the channel or an accessory protein is required for its activation. In this study, the effect of intracellular ATP depletion on the swelling-induced release of D-[3H]aspartate from rat primary astrocyte cultures due to exposure to either high K(+) or hypotonic media was studied. When the cells were pretreated for 10 min with a combination of the metabolic inhibitors 2-deoxyglucose and rotenone, 100 mM K(+) media- or hypotonic media-induced D-[3H]aspartate release was completely suppressed. Added separately, each inhibitor showed only partial or no inhibition of D-[3H]aspartate release, which correlated with its relative effectiveness in decreasing intracellular ATP levels. These data are consistent with the view that during high [K(+)](o) or hypotonic media-induced swelling of primary astrocyte cultures an ATP-dependent swelling-activated VSOAC channel is responsible for D-[3H]aspartate release and close to normal ATP is required for full channel activation.
authors.affiliation: Department of Pharmacology and Neuroscience, Albany Medical College, Albany, NY 12208, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Rutledge
authors.forename: A A
authors.initials: AA
authors.lastname: Mongin
authors.forename: H K
authors.initials: HK
authors.lastname: Kimelberg
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001224:Aspartic Acid / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism* / Q000648:ultrastructure; D048429:Cell Size; D002478:Cells, Cultured; D007038:Hypotonic Solutions; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000963:Antimetabolites; D007038:Hypotonic Solutions; D001224:Aspartic Acid; D000255:Adenosine Triphosphate; D011188:Potassium