title: Opposite modulation of capsaicin-evoked substance P release by glutamate receptors.
abstract: Substance P and glutamate are present in primary afferent C-fibers and play important roles in persistent inflammatory and neuropathic pain. In the present study, we have examined whether activation of different glutamate receptor subtypes modulates the release of substance P evoked by the C-fiber selective stimulant capsaicin (1 microM) from rat trigeminal nucleus slices. The selective NMDA glutamate receptor agonist L-CCG-IV (1-10 microM) enhanced capsaicin-evoked substance P release about 100%. This facilitatory effect was blocked by 0.3 microM MK-801, a selective NMDA receptor antagonist. The metabotropic glutamate receptor agonists L-AP4 (group III) and DHPG (group I) (30-100 microM) inhibited capsaicin-evoked substance P release by approximately 60%. These inhibitory effects were blocked by the selective metabotropic glutamate receptor antagonist (+/-)-MCPG (5 microM). On the other hand, AMPA and kainate (0.1-10 microM), did not significantly affect capsaicin-evoked substance P release. Thus, substance P release from non-myelinated primary afferents, and possibly nociception, may be under the functional antagonistic control of some metabotropic and ionotropic glutamate receptor subtypes.
authors.affiliation: Section of Pharmacology, Instituto de Investigaciones Clinicas, University of Zulia, Maracaibo, Venezuela.
authors.forename: M C
authors.initials: MC
authors.lastname: Cuesta
authors.forename: J L
authors.initials: JL
authors.lastname: Arcaya
authors.forename: G
authors.initials: G
authors.lastname: Cano
authors.forename: L
authors.initials: L
authors.lastname: Sanchez
authors.forename: W
authors.initials: W
authors.lastname: Maixner
authors.forename: H
authors.initials: H
authors.lastname: Suarez-Roca
mesh_terms: D000344:Afferent Pathways / Q000187:drug effects; D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D009412:Nerve Fibers / Q000187:drug effects* / Q000378:metabolism; D009619:Nociceptors / Q000502:physiology; D011422:Propionates / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000187:drug effects*; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000037:antagonists & inhibitors; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000037:antagonists & inhibitors; D013373:Substance P / Q000378:metabolism*; D014278:Trigeminal Nuclei / Q000187:drug effects* / Q000378:metabolism; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
chemical_list: C114785:2-amino-4-phosphono-propinate; D001565:Benzoates; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D011422:Propionates; D017470:Receptors, Glutamate; D018094:Receptors, Metabotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; C079694:alpha-methyl-4-carboxyphenylglycine; D013373:Substance P; D008734:Methoxyhydroxyphenylglycol; D016291:Dizocilpine Maleate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D002211:Capsaicin; D007608:Kainic Acid; D005998:Glycine; C010117:3,4-dihydroxyphenylglycol