title: Successful immunotherapy of an intraocular tumor in mice.
abstract: Immune privilege in the eye is considered essential in the protection against local sight-threatening inflammatory responses. However, the deviant immune responses in the eye may also provide an ideal opportunity to uncontrolled growth of viruses or tumors by inhibiting intraocular immunological attack. To establish to what extent immune privilege interferes with T cell-mediated antitumor immunotherapy, we established a new ocular tumor model in the mouse and tested whether well-defined tumor-specific CTLs can eradicate an immunogenic intraocularly growing tumor. Tumor cells, transformed by human adenovirus type 5 early region 1 (Ad5E1), injected s.c. in a dose of 10(7) cells, did not induce s.c. tumor growth in C57BL/6 mice. However, an injection of 0.3 x 10(6) of these cells into the anterior chamber of the eye led to intraocular tumor growth in 95% of mice (n = 20). Tumor growth in the eye did not induce systemic tumor-specific tolerance, because 70% of the mice were able to eradicate the tumor spontaneously after 5 weeks. Mice vaccinated s.c. with irradiated tumor cells were protected against intraocular tumor challenge, indicating that preactivated memory T cells are able to protect against intraocular tumor growth. Moreover, an i.v. injection of an Ad5E1-specific CTL clone was able to eradicate established intraocular Ad5E1-transformed tumors, whereas the anatomy of the eye remained intact. These results demonstrate that tumor-specific, CTL-mediated immunity can be used successfully for the prevention and eradication of tumors growing in the immune-privileged anterior chamber of the eye, without detectable destruction of the eye.
authors.affiliation: Department of Ophthalmology, Leiden University Medical Center, The Netherlands.
authors.forename: L R
authors.initials: LR
authors.lastname: Schurmans
authors.forename: A T
authors.initials: AT
authors.lastname: den Boer
authors.forename: L
authors.initials: L
authors.lastname: Diehl
authors.forename: E I
authors.initials: EI
authors.lastname: van der Voort
authors.forename: W M
authors.initials: WM
authors.lastname: Kast
authors.forename: C J
authors.initials: CJ
authors.lastname: Melief
authors.forename: R E
authors.initials: RE
authors.lastname: Toes
authors.forename: M J
authors.initials: MJ
authors.lastname: Jager
mesh_terms: D000260:Adenoviruses, Human / Q000276:immunology; D000818:Animals; D000867:Anterior Chamber / Q000276:immunology; D005134:Eye Neoplasms / Q000276:immunology / Q000628:therapy*; D007114:Immunization; D016219:Immunotherapy, Adoptive; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*