Molecular cloning of Arabidopsis photolyase gene (PHR1) and characterization of its promoter region.
Photolyase is an enzyme that repairs ultraviolet (UV)-damaged DNA by photoreactivation. In higher plants, accumulation of photolyase (PHR1) mRNA is induced by either UV or visible light. In order to know the molecular mechanism by which PHR1 gene expression is induced by light, we have determined the genomic structure and the 5'-flanking sequence of the Arabidopsis PHR1 gene. The PHR1 gene spans approximately 2.5 kb of genomic DNA and consists of 9 exons. In the promoter region of PHR1, there are two pairs of inverted repeats spanning more than sixty base pairs. The promoter also contains DNA motifs similar to the GT-1 box or G-box found in many light-inducible gene promoters. EMSA analysis showed that several proteins in Arabidopsis nuclear extract bound to the G-box-like motifs. These results raise the possibility that the Arabidopsis PHR1 gene is regulated by transcription factors which interact with these motifs.
Sakamoto
A
A
Research Group for Plant Genes, Advanced Science Research Center, Japan Atomic Energy Research Institute, Takasaki, Gunma. sakamoto@taka.jaeri.go.jp
Tanaka
A
A
Watanabe
H
H
Tano
S
S
D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000737:chemistry / Q000235:genetics*; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D005656:Fungal Proteins; D007438:Introns; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D020121:5' Untranslated Regions; D001051:Apoenzymes; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C091515:PHR1 protein, Candida albicans; D004255:Deoxyribodipyrimidine Photo-Lyase