Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats.
In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.
Zhang
W R
WR
Department of Neurology, Okayama University Medical School, 2-5-1 Shikatacho, Okayama, Japan.
Kitagawa
H
H
Hayashi
T
T
Sasaki
C
C
Sakai
K
K
Warita
H
H
Shiro
Y
Y
Suenaga
H
H
Ohmae
H
H
Tsuji
S
S
Itoh
T
T
Nishimura
O
O
Nagasaki
H
H
Abe
K
K
D000287:Administration, Topical; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D002560:Cerebrovascular Circulation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D020768:Middle Cerebral Artery / Q000502:physiology*; D020933:Neurotrophin 3 / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D020933:Neurotrophin 3; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases