Emotional stress induces immediate-early gene expression in rat heart via activation of alpha- and beta-adrenoceptors.
We have studied the adrenergic mechanisms of immediate-early gene (IEG) induction in the discrete types of cardiac cells with the use of in situ hybridization histochemistry in an immobilization-stress model in conscious rats. Expression of c-fos, fos B, c-jun, jun B, NGFI-A, and NGFI-B mRNA was rapidly upregulated in the endothelial, myocardial, and smooth muscle cells of coronary vessels by 15-45 min after the onset of immobilization. Simultaneous blockade of both alpha- and beta-adrenoceptors completely abolished expression of IEGs in these cardiac cells. Application of an alpha-agonist or beta-agonist alone to the perfused rat heart under constant pressure elicited the upregulation of IEGs in a fashion similar to that of emotional stress. These data suggest that activation of either alpha- or beta-adrenoceptor is sufficient to evoke expression of these genes and that there may be cross talk in signal transduction downstream from alpha- and beta-adrenoceptors in cardiac cells.
Ueyama
T
T
Department of Anatomy, Wakayama Medical College, Wakayama 641-8509, Japan. tueyama@wakayama-med.ac.jp
Yoshida
K
K
Senba
E
E
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D003331:Coronary Vessels / Q000166:cytology / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology; D015870:Gene Expression; D017781:Genes, Immediate-Early / Q000235:genetics*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D013315:Stress, Psychological / Q000235:genetics*; D015854:Up-Regulation
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D012333:RNA, Messenger; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta