[Study of disorders of the GH-IGF-I axis by monitoring levels of IGF-I binding protein].
Measurement of IGF I (Insulin Like Growth Factor I) and its binding proteins (IGFBP) in serum has become a principal diagnostic tool at the determination of growth hormone (GH) status. These data could, at the correct interpretation, fully replace the stimulation tests necessary for the GH determination. A lot of firms were interested in the analysis of IGFBPs, most frequently based on the immunological principle between IGFBP and its antibody. The assessment of IGFBP-3 is the most spread among all IGFBPs in the studies of GH-IGF I axis. It is used both at the GH deficiency, and at the GH access, it has become a valuable marker at the therapy of children suffering from GH insufficiency. IGFBPs analysis based on electrophoresis and Western blotting with the following detection of ligan-IGFBP by radioisotop or by its immunological reaction with antibody plays an important role at the clinical application research. The changes in IGFBP profile or in their fragments can be an important markers (even specific one) at different disease. The contemporary research in IGFBPs domain is fully concentrated to the IGFBPs action in cells. It has been found f.e., that IGFBPs inhibit the growth of carcinomic cells.
Justová
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Laborator pro endokrinologii a metabolismus, lékarské fakulty Univerzity Karlovy, Praha, Czech Republic.
Lacinová
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Z
D000328:Adult; D000368:Aged; D013006:Growth Hormone / Q000097:blood*; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D008875:Middle Aged
D018970:Insulin-Like Growth Factor Binding Protein 1; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone