title: Previously reported nerve growth factor levels are underestimated due to an incomplete release from receptors and interaction with standard curve media.
abstract: The 1996 research report by Hoener et al. [M.C. Hoener, E. Hewitt, J. M. Conner, J.W. Costello, S. Varon, Nerve growth factor (NGF) content in adult rat brain tissue is several-fold higher than generally reported and is largely associated with sedimentable fractions, Brain Res. 728 (1996) 47-56.] compares levels of nerve growth factor (NGF) found in rat brain by assaying both supernatant and pellet to previously reported data. However, Hoener et al. miscalculated when converting values previously reported in the literature to units of picogram per milliliter. Regardless of this mistake, the method of tissue extraction does affect the extent of release of NGF, which must be maximized in order to accurately determine NGF levels in the central nervous system. We now report that accurate measurement of NGF levels is not only affected by the incomplete release of NGF from receptors, but also the medium in which the standard curve is run. It is the combination of these two variables that has led to the underestimation of NGF levels in previous research.
authors.affiliation: Department of Neurology, The Alzheimer's Treatment and Research Center, HealthPartners, Regions Hospital, 640 Jackson Street, St. Paul, MN 55101-2595, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Fawcett
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: Y E
authors.initials: YE
authors.lastname: Rahman
authors.forename: W H
authors.initials: WH
authors.lastname: Frey
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001923:Brain Chemistry; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D009414:Nerve Growth Factors / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D009414:Nerve Growth Factors; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins