title: Transglycosylation of cellobiose by partially purified Trichoderma viride cellulase.
abstract: A commercial cellulase from Trichoderma viride was fractionated into three fractions, F1, F2, and F3, in order to investigate transglycosylation activities. Among these fractions, F3, which demonstrated highly hydrolytic activity toward p-nitrophenyl beta-D-glucopyranoside and Avicel, most effectively catalyzed the transglycosylation of cellobiose and converted cellobiose into beta-Glc-(1-->6)-beta-glc-(1-->4)-Glc and beta-Glc-(1-->6)-beta-Glc-(1-->6)-beta-Glc(1-->4)-Glc. The F3 fraction contained the enzyme to catalyze beta-glucosyl transfer toward only the C-6 position of the sugar acceptor, and thus it is expected to be of use for syntheses of functional oligosaccharides.
authors.affiliation: Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan. kohno@dove-mc.eng.hokudai.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Kono
authors.forename: M R
authors.initials: MR
authors.lastname: Waelchli
authors.forename: M
authors.initials: M
authors.lastname: Fujiwara
authors.forename: T
authors.initials: T
authors.lastname: Erata
authors.forename: M
authors.initials: M
authors.lastname: Takai
mesh_terms: D002240:Carbohydrate Sequence; D002475:Cellobiose / Q000737:chemistry*; D002480:Cellulase / Q000302:isolation & purification*; D005591:Chemical Fractionation; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D009844:Oligosaccharides; D002475:Cellobiose; D002480:Cellulase