Training ribozymes to switch.
Ribozymes that are sensitive to cyclic nucleotides have been selected in vitro. Remarkably, the cGMP-dependent ribozymes are specifically activated by a factor of 5,000 - the largest allosteric ribozyme activation by a small molecule seen to date.
Marshall
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Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.
Ellington
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D000494:Allosteric Regulation / Q000187:drug effects; D002384:Catalysis / Q000187:drug effects; D006152:Cyclic GMP / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005818:Genetic Engineering / Q000379:methods*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D016337:RNA, Catalytic; D006152:Cyclic GMP