title: Application of sulfobutylether-beta-cyclodextrin with specific degrees of substitution for the enantioseparation of pharmaceutical mixtures by capillary electrophoresis.
abstract: In this research the separation of the enantiomers of the basic drug bidisomide (SC-40230) from five closely related known process impurities was investigated using several neutral and anionic sulfobutylether beta-cyclodextrins (SBE-beta-CDs) as isomer selectors. Several novel sulfobutylether derivative mixtures and purified charge types having a specific degree of substitution were used to study the effect of selector charge on the efficiency and selectivity of both chiral and achiral separations. The effects of run buffer pH, selector type, and selector concentration on the chiral separation of bidisomide and the achiral separation of the related process impurities was also investigated. The related process impurity, SC-47500, displayed significant peak tailing with SBE-beta-CD mixtures which contained mono- to deca-substituted cyclodextrins. This problem was explored using isolated SBE-beta-CD charge types having degrees of substitution from one to seven. Peak tailing increased as the charge on the selector increased, suggesting that the distortion was due to electrodispersion and the large countercurrent mobility of the negatively charged complexes. Pure charge types having a lower degree of substitution provided adequate chiral and achiral selectivity, while eliminating the severe peak distortion caused by electrodispersion. The complete analysis of the bidisomide enantiomers and the related impurities was achieved with a pH 2.5 running buffer containing 5-10 mM of the isolated sulfobutylether charge types SBE[2]ds(1)sr-beta-CD or SBE[3]ds(1)sr-beta-CD. These conditions gave baseline resolution of bidisomide enantiomers and all five impurities, thus allowing both chiral and achiral purity to be determined in a single run.
authors.affiliation: Department of Pharmaceutical Chemistry, Center for Bioanalytical Research, The University of Kansas, Lawrence 66047, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Skanchy
authors.forename: G H
authors.initials: GH
authors.lastname: Xie
authors.forename: R J
authors.initials: RJ
authors.lastname: Tait
authors.forename: E
authors.initials: E
authors.lastname: Luna
authors.forename: C
authors.initials: C
authors.lastname: Demarest
authors.forename: J F
authors.initials: JF
authors.lastname: Stobaugh
mesh_terms: D000889:Anti-Arrhythmia Agents / Q000302:isolation & purification*; D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004987:Ethers / Q000737:chemistry*; D009994:Osmolar Concentration; D010880:Piperidines / Q000302:isolation & purification*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
chemical_list: D000889:Anti-Arrhythmia Agents; D003505:Cyclodextrins; D004987:Ethers; D010880:Piperidines; D047392:beta-Cyclodextrins; C093196:SBE4-beta-cyclodextrin; C061991:bidisomide