Disruption of sblA in Streptomyces lividans permits expression of a heterologous alpha-amylase gene in the presence of glucose.
In a transposition mutant of Streptomyces lividans TK24, the usually glucose-repressible expression of a heterologous alpha-amylase gene (aml) became resistant to glucose repression. The transposon had inserted into an ORF called sblA which encodes a 274 aa product sharing significant sequence similarities with various phosphatases that act on small phosphorylated substrates. sblA was transcribed as a monocistronic mRNA and its transcription was enhanced at the transition phase. Because its transcriptional and putative translational start points coincide, sblA is likely to be translated in the absence of a conventional RBS. The sblA-disrupted mutant is characterized by early growth arrest in glucose-grown cultures and by partial relief of glucose repression of aml expression.
Gagnat
Josette
J
Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, CNRS UMR8621 Bâtiment 400, Université Paris-Sud, F-91405 Orsay Cedex, France1.
Chouayekh
Hichem
H
Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, CNRS UMR8621 Bâtiment 400, Université Paris-Sud, F-91405 Orsay Cedex, France1.
Gerbaud
Claude
C
Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, CNRS UMR8621 Bâtiment 400, Université Paris-Sud, F-91405 Orsay Cedex, France1.
Francou
François
F
Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, CNRS UMR8621 Bâtiment 400, Université Paris-Sud, F-91405 Orsay Cedex, France1.
Virolle
Marie-Joelle
MJ
Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, CNRS UMR8621 Bâtiment 400, Université Paris-Sud, F-91405 Orsay Cedex, France1.
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003470:Culture Media; D004251:DNA Transposable Elements; D017353:Gene Deletion; D015870:Gene Expression; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D004251:DNA Transposable Elements; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase; D000516:alpha-Amylases; D005947:Glucose