title: Simultaneous determination of chlordiazepoxide and clidinium bromide in pharmaceutical formulations by derivative spectrophotometry.
abstract: A direct and simple first derivative spectrophotometric method has been developed for the simultaneous determination of clidinium bromide and chlordiazepoxide in pharmaceutical formulations. Acetonitrile was used as solvent for extracting the drugs from the formulations and subsequently the samples were evaluated directly by derivative spectrophotometry. Simultaneous determination of the drugs can be carried out using the zero-crossing method for clidinium bromide at 220.8 nm and the graphical method for chlordiazepoxide at 283.6 nm. The calibration graphs were linear in the ranges from 0.983 to 21.62 mg/l of clidinium bromide and from 0. 740 to 12.0 mg/l of chlordiazepoxide. The ingredients commonly found in commercial pharmaceutical formulations do not interfere. The proposed method was applied to the determination of these drugs in tablets.
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Chile, P. O. Box 653, Santiago, Chile.
authors.forename: M I
authors.initials: MI
authors.lastname: Toral
authors.forename: P
authors.initials: P
authors.lastname: Richter
authors.forename: N
authors.initials: N
authors.lastname: Lara
authors.forename: P
authors.initials: P
authors.lastname: Jaque
authors.forename: C
authors.initials: C
authors.lastname: Soto
authors.forename: M
authors.initials: M
authors.lastname: Saavedra
mesh_terms: D000097:Acetonitriles; D002626:Chemistry, Pharmaceutical; D002707:Chlordiazepoxide / Q000032:analysis*; D006993:Hypnotics and Sedatives / Q000032:analysis*; D010276:Parasympatholytics / Q000032:analysis*; D011813:Quinuclidinyl Benzilate / Q000031:analogs & derivatives* / Q000032:analysis; D012680:Sensitivity and Specificity; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets / Q000032:analysis
chemical_list: D000097:Acetonitriles; D006993:Hypnotics and Sedatives; D010276:Parasympatholytics; D012997:Solvents; D013607:Tablets; D011813:Quinuclidinyl Benzilate; D002707:Chlordiazepoxide; C054940:clidinium