title: Analysis of cyclophosphamide and five metabolites from human plasma using liquid chromatography-mass spectrometry and gas chromatography-nitrogen-phosphorus detection.
abstract: An assay method for the quantification of cyclophosphamide (CY) and five metabolites from human plasma is presented. The procedure is adapted to the chemical properties of the compounds of interest: non-polar compounds are extracted into methylene chloride, concentrated and analyzed by GC-NPD after derivatization, and the remaining aqueous fraction is deproteinated with acetonitrile-methanol prior to separation via reversed-phase HPLC and detection using atmospheric pressure ionization (API)-MS. Standard curves are linear over the required range and reproducible over five months. Plasma concentration-time profiles of CY and metabolites from a patient receiving CY by intravenous infusion (60 mg/kg, once a day for two days) are presented.
authors.affiliation: Department of Pharmaceutics, University of Washington, Seattle 98195, USA.
authors.forename: T F
authors.initials: TF
authors.lastname: Kalhorn
authors.forename: S
authors.initials: S
authors.lastname: Ren
authors.forename: W N
authors.initials: WN
authors.lastname: Howald
authors.forename: R F
authors.initials: RF
authors.lastname: Lawrence
authors.forename: J T
authors.initials: JT
authors.lastname: Slattery
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003520:Cyclophosphamide / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis
chemical_list: D018906:Antineoplastic Agents, Alkylating; D010758:Phosphorus; D003520:Cyclophosphamide; D009584:Nitrogen