title: Ca2+ sensitivity of stunned myocardium after skinning using retrograde infusion of detergent.
abstract: Myofilament Ca2+ desensitization contributes to the contractile dysfunction of ischemic/reperfused ("stunned") myocardium. We examined the presence of reduced Ca2+ sensitivity of isometric force in chemically skinned fibers obtained from stunned myocardium using different modes of applying the detergent Triton X-100. Langendorff-perfused rat hearts underwent 20 min ischemia/20 min reperfusion, which caused a 35 +/- 3% decrease in left ventricular developed pressure, compared to continuously perfused control hearts. Stunned and control hearts were randomly allocated to two different permeabilization protocols: In group A, trabeculae were dissected and immersed in skinning solution containing 1% Triton X-100 for 20 min. Group B hearts remained fixed to the aortic cannula and skinning solution was infused retrogradely for 6 min prior to dissection of trabeculae. Extraction of cytosolic marker proteins was more complete in group-B than in group-A preparations. Group-A preparations from stunned hearts exhibited significant Ca2+ desensitization (pCa50 = 5.07 and 5.15 in stunned and control myocardium, respectively). In group B no such difference was observed, all preparations showing higher Ca2+ sensitivity and maximum force than group-A preparations (pCa50 = 5.32 in stunned versus 5.33 in control hearts). Prolonging group-A skinning to 150 min also abolished the difference in Ca2+ sensitivity between stunned and control myocardium. In conclusion, compared to a conventional protocol, skinning by perfusion results in more complete permeabilization and better preservation of myocardial contractile function. Ischemia/reperfusion at this moderate degree of contractile dysfunction induces Ca2+ desensitization at least partially by factors that can be extracted by thorough skinning.
authors.affiliation: Institute of Physiology II, University of Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Geyer
authors.forename: J D
authors.initials: JD
authors.lastname: Strauss
authors.forename: J C
authors.initials: JC
authors.lastname: Rüegg
authors.forename: H
authors.initials: H
authors.lastname: Kögler
mesh_terms: D000818:Animals; D015415:Biomarkers; D002118:Calcium / Q000502:physiology*; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology; D003600:Cytosol / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology*; D005260:Female; D006439:Hemodynamics; D006652:Histological Techniques; D066298:In Vitro Techniques; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009200:Myocardial Contraction / Q000502:physiology*; D017202:Myocardial Ischemia / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology; D017682:Myocardial Stunning; D017830:Octoxynol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019210:Troponin I / Q000378:metabolism
chemical_list: D015415:Biomarkers; D003902:Detergents; D008565:Membrane Proteins; D019210:Troponin I; D017830:Octoxynol; D002118:Calcium