Identification of false-positive CBFbeta/MYH11 RT-PCR results.
Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBFbeta/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBFbeta3 primer to intronic MYH11 sequence.
Hackwell
S M
SM
Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK.
Robinson
D O
DO
Harvey
J F
JF
Ross
F M
FM
D000208:Acute Disease; D016022:Case-Control Studies; D007446:Chromosome Inversion; D020732:Cytogenetic Analysis; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D011237:Predictive Value of Tests; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction
C095514:CBFbeta-MYH11 fusion protein; D015514:Oncogene Proteins, Fusion