title: A mouse model utilising human transferrin to study protection against Neisseria meningitidis serogroup B induced by outer membrane vesicle vaccination.
abstract: We have previously developed a mouse model based on transient bacteraemia in normal B10.M mice to evaluate the protective efficacy of outer membrane vesicle vaccines against serogroup B meningococci. To obtain a course of infection similar to that observed in man, we have in this work modified the mouse model by administration of human holo-transferrin upon bacterial challenge. Co-challenge with holo-transferrin induced increasing bacteraemia and subsequent death in normal non-immune mice, but not in vaccinated animals. The model system is dependent on challenge with meningococci expressing the transferrin receptor which is obtained by culturing the bacteria under iron restriction. The modified model system for protection against meningococcal infection presented here makes it possible to measure outer membrane vesicle vaccine induced protection by using bacteraemia as well as survival as parameters.
authors.affiliation: Department of Vaccinology, The National Institute of Public Health, P.O. Box 4404 Torshov, N-0403, Oslo, Norway. fredrik.oftung@folkehelsa.no
authors.forename: F
authors.initials: F
authors.lastname: Oftung
authors.forename: M
authors.initials: M
authors.lastname: Lovik
authors.forename: S R
authors.initials: SR
authors.lastname: Andersen
authors.forename: L O
authors.initials: LO
authors.lastname: Froholm
authors.forename: G
authors.initials: G
authors.lastname: Bjune
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005688:Galactosamine / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000494:pharmacology; D008585:Meningitis, Meningococcal / Q000097:blood / Q000401:mortality / Q000517:prevention & control*; D051379:Mice; D020297:Mice, Congenic; D009345:Neisseria meningitidis / Q000276:immunology*; D015996:Survival Rate; D014168:Transferrin / Q000494:pharmacology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D014168:Transferrin; D005688:Galactosamine; D007501:Iron