title: Effect of protein intake on plasma and erythrocyte free amino acids and serum IGF-I and IGFBP-1 levels in rats.
abstract: Amino acid (AA) levels in plasma and erythrocytes (RBC) were determined in rats (n = 29) fed diets with 6, 21, and 35% protein, and their association with insulin-like growth factor I (IGF-I), insulin, or IGF-binding protein (IGFBP)-1 levels was studied. Free AA in plasma and RBC were determined by reversed-phase high-pressure liquid chromotography, and IGF-I, IGFBP-1, and insulin plasma levels were determined by RIA. Rats fed the low-protein (6%) diet were growth-retarded and had lower serum IGF-I levels and higher serum IGFBP-1 levels than the other two groups (P < 0.0001). In rats fed the low-protein diet, most of the nonessential AA (NEAA) in both plasma and RBC increased, whereas the essential AA (EAA), with the exception of threonine, decreased. When the groups were combined, both RBC and plasma EAA-to-NEAA ratios were positively correlated to IGF-I (r = 0.76 and 0.80, respectively; P < 0.0001) and inversely correlated to IGFBP-1 levels (r = -0.67, P < 0.001 and r = -0.78, P < 0.0001, respectively). A significant inverse correlation was found between RBC glutamate and IGF-I (r = -0.85, P < 0.0001, n = 25) and insulin (r = -0.72, P < 0.001, n = 21), and a positive correlation was found for IGFBP-1 (r = 0.78, P < 0.0001, n = 24). In multiple regression analysis, only IGF-I remained as an independent variable. Threonine was the only EAA with a significant inverse correlation to insulin (r = -0.66, P < 0.001). We hypothesize that AA metabolism is associated to changes in IGF-I, insulin, and IGFBP-1 levels in rats on different protein intakes.
authors.affiliation: Division of Renal Medicine, Department of Clinical Science, Karolinska Hospital, Karolinska Institutet, S-141 86 Stockholm, Sweden. jose.divino@klinvet.ki.se
authors.forename: J C
authors.initials: JC
authors.lastname: Filho
authors.forename: S J
authors.initials: SJ
authors.lastname: Hazel
authors.forename: B
authors.initials: B
authors.lastname: Anderstam
authors.forename: J
authors.initials: J
authors.lastname: Bergström
authors.forename: M
authors.initials: M
authors.lastname: Lewitt
authors.forename: K
authors.initials: K
authors.lastname: Hall
mesh_terms: D000375:Aging / Q000097:blood; D000596:Amino Acids / Q000097:blood*; D000818:Animals; D004044:Dietary Proteins / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D007328:Insulin / Q000097:blood; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D051381:Rats / Q000235:genetics; D017207:Rats, Sprague-Dawley; D014508:Urea / Q000097:blood
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; D007334:Insulin-Like Growth Factor I; D014508:Urea