A cell-free assay system for beta-catenin signaling that recapitulates direct inductive events in the early xenopus laevis embryo.
In vertebrate embryos, signaling via the beta-catenin protein is known to play an essential role in the induction of the dorsal axis. In its signaling capacity, beta-catenin acts directly to affect target gene transcription, in concert with transcription factors of the TCF/LEF family. We have developed a cell-free in vitro assay for beta-catenin signaling activity that utilizes transcriptionally active nuclei and cytoplasm from cleavage-blocked Xenopus laevis embryos. Under these assay conditions, we demonstrate that either addition of beta-catenin protein or upstream activation of the beta-catenin signaling pathway can induce the expression of developmentally relevant target genes. Addition of exogenous beta-catenin protein induced expression of Siamois, XTwin, Xnr3, and Cerberus mRNAs in a protein synthesis independent manner, whereas a panel of other Spemann organizer-specific genes did not respond to beta-catenin. Lithium induction of the beta-catenin signaling pathway, which is thought to cause beta-catenin accumulation by inhibiting its proteasome-dependent degradation, caused increased expression of Siamois in a protein synthesis independent fashion. This result suggests that beta-catenin derived from a preexisting pool can be activated to signal, and that accumulation of this activated form does not require ongoing synthesis. Furthermore, activation of the signaling pathway with lithium did not detectably alter cytoplasmic beta-catenin levels and was insensitive to inhibition of the proteasome- dependent degradation pathway. Taken together, these results suggest that activation of beta-catenin signaling by lithium in this system may occur through a distinct activation mechanism that does not require modulation of levels through regulation of proteasomal degradation.
Nelson
R W
RW
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Gumbiner
B M
BM
D000818:Animals; D001681:Biological Assay; D002474:Cell-Free System; D003598:Cytoskeletal Proteins / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology*; D015398:Signal Transduction; D015534:Trans-Activators; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000502:physiology; D051176:beta Catenin
C495266:CTNNB1 protein, Xenopus; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D029867:Xenopus Proteins; D051176:beta Catenin