Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane.
We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 microsecond and lifetime components as long as 3 microseconds in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2 x 10(-8) cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with microsecond lipid probes.
Li
L
L
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
Gryczynski
I
I
Lakowicz
J R
JR
D004735:Energy Transfer; D008024:Ligands; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D012211:Rhenium; D013054:Spectrophotometry, Atomic
D008024:Ligands; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D012211:Rhenium; C017251:1,2-oleoylphosphatidylcholine