title: Lipolysis induced by alloxan in rat adipocytes is not inhibited by insulin.
abstract: Isolated rat adipocytes were incubated with adrenaline, adrenaline plus insulin, alloxan or alloxan plus insulin. Glycerol release was taken as a measure of lipolysis. It was observed that alloxan in the concentration of 3, 10 and 20 mmol/l intensifies lipolysis in adipocytes in the absence of adrenaline. Insulin (10(-6) mol/l) treatment of cells did not inhibit lipolysis caused by this compound, but significantly restricted lipolysis induced by adrenaline (10(-6) mol/l). It was also shown that alloxan in the concentration of 3 and 10 mmol/l intensified lipolysis stimulated by adrenaline (10(-6) mol/l). Addition of 20 mmol/l of alloxan strongly inhibited glycerol release in the presence of adrenaline. The results presented here clearly indicate that the action of alloxan concerns cells of the white adipose tissue.
authors.affiliation: Department of Animal Physiology and Biochemistry, University of Agriculture, Poznań, Poland. kakandu@jay.au.poznan.pl
authors.forename: K
authors.initials: K
authors.lastname: Kandulska
authors.forename: T
authors.initials: T
authors.lastname: Szkudelski
authors.forename: L
authors.initials: L
authors.lastname: Nogowski
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004347:Drug Interactions; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D000496:Alloxan