PDGF-AB release during and after haemodialysis procedure.
BACKGROUND
During haemodialysis blood membrane contact causes the release of the content of platelet alpha-granules, which contain platelet-derived growth factor (PDGF). In view of its possible role in accelerated atherosclerotic processes, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during haemodialysis sessions performed using a cellulosic membrane.


METHODS
Using the ELISA method, PDGF-AB, platelet factor-4 (PF4) and beta-thromboglobulin (beta-TG) levels were determined in peripheral blood, as well as in arterial and venous haemodialyser lines, in 10 patients each of whom underwent five consecutive dialysis sessions with a CU membrane. Blood samples were taken at 0, 15, 30, 60, 120, 180 and 240 min during dialysis and at 1, 4 and 20 h after the end of the session. In the same group of patients the levels of the same molecules were also determined after a heparin bolus injection of 4500 IU, blood samples were taken at 0, 15 and 30 min after injection of the bolus.


RESULTS
PDGF-AB serum levels increased, remained consistently high during the haemodialysis session (in particular +134+/-20% after 30 min, P<0.001, and +140+/-5% after 240 min, P<0.001) and returned to basal values only after 20 h following the end of the session. PF4 and beta-TG showed a similar trend to PDGF. The heparin bolus injection caused only a small increase (+15+/-5% at 30 min) in PDGF-AB serum levels.


CONCLUSIONS
PDGF-AB is released during dialysis mainly as consequence of the blood-membrane contact and it returns only slowly to basal values.
Cianciolo
G
G
Institute of Nephrology, St. Orsola University Hospital, Bologna, Italy.
Stefoni
S
S
Zanchelli
F
F
Iannelli
S
S
Colì
L
L
Borgnino
L C
LC
De Sanctis
L B
LB
Stefoni
V
V
De Pascalis
A
A
Isola
E
E
La Hanna
G
G
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D006435:Renal Dialysis; D001620:beta-Thromboglobulin / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D001620:beta-Thromboglobulin; C095477:platelet-derived growth factor AB; D010978:Platelet Factor 4