Protein kinase C-alpha is essential for Ramos-BL B cell survival.
The Ramos-Burkitt lymphoma (BL) B cell line is driven into growth arrest and apoptosis by cross-linking surface immunoglobulin. We demonstrate that protein kinase C (PKC) activity is required for Ramos B cell proliferation and survival. A variety of PKC inhibitors trigger a significant decrease in [(3)H]thymidine incorporation with a concomitant increase in cell death. Antisense depletion of expression of the PKC-alpha isoform is sufficient to trigger cell death in the absence of any other signal, demonstrating a requirement for this isoform for survival of Ramos-BL B cells. Cross-linking surface immunoglobulin also leads to depletion of PKC-alpha levels, suggesting that this may be one mechanism by which this signals for cell death in Ramos-BL B cells.
Keenan
C
C
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom.
Thompson
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S
Knox
K
K
Pears
C
C
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D017209:Apoptosis; D001402:B-Lymphocytes / Q000201:enzymology* / Q000473:pathology; D001426:Bacterial Proteins / Q000494:pharmacology; D002051:Burkitt Lymphoma / Q000473:pathology*; D002455:Cell Division; D002470:Cell Survival; D002675:Child, Preschool; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007152:Immunologic Capping; D007211:Indoles / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D008297:Male; D008301:Maleimides / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000201:enzymology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D051571:Protein Kinase C-alpha; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D007211:Indoles; D007527:Isoenzymes; D008301:Maleimides; D009281:Naphthalenes; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms; D011947:Receptors, Antigen, B-Cell; C044203:anti-IgM; C061465:calphostin complex; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; C070515:bisindolylmaleimide I