title: Synthesis of an artificial glycoconjugate polymer carrying Pk-antigenic trisaccharide and its potent neutralization activity against Shiga-like toxin.
abstract: Fluorescence-labeled glycoconjugate polymers carrying carbohydrate segments of a globotriaosyl ceramide (Gb3) were synthesized and subjected to biological assays using Escherichia coli O-157 strains and Shiga-like toxins (Stx-I and Stx-II). For the fluorescence labeling, a new polymerizable fluorescent monomer with a TBMB carbonyl chromophore (Ex. 325 nm, Em. 410 nm) was designed. A glycosyl monomer of the trisaccharide segment of Gb3 was prepared from p-nitrophenyl beta-lactoside and copolymerized with acrylamide and the fluorescent monomer to prepare a fluorescence-labeled glycoconjugate copolymer carrying [alpha-D-galactopyranosyl-(1-->4)-beta-D-galactopyranosyl]-(1-->4)-beta- D-glucopyranoside. The polymer showed potent neutralization activity against Stx-I and also binding activity onto E. coli O-157 strains.
authors.affiliation: Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Dohi
authors.forename: Y
authors.initials: Y
authors.lastname: Nishida
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: M
authors.initials: M
authors.lastname: Shinkai
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Uzawa
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D019453:Escherichia coli O157 / Q000276:immunology; D005453:Fluorescence; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology; D011108:Polymers; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D013057:Spectrum Analysis
chemical_list: D000941:Antigens; D001427:Bacterial Toxins; D006001:Glycoconjugates; D009844:Oligosaccharides; D011108:Polymers; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2