Plant tissue culture as a model for study of diversity in formaldehyde binding.
The effect of endogenous formaldehyde (HCHO) deprivation with dimedone - as abiotic stress - was investigated on the methylation-demethylation reactions in Datura innoxia Mill. callus cultures. The matrix assisted laser desorption/ionization mass spectrometric (MALDI MS) investigation of the culture extracts revealed characteristic differences between the tissues cultivated in light and dark. MALDI MS data show the presence of a precursor molecule and its mono- and tri-hydroxymethyl derivative, only in the cultures maintained in light. The relative amount of the different derivatives changed considerably, as a consequence of dimedone application in the culture medium and during the extraction.
László
I
I
Institute of Pharmacognosy, Semmelweis University of Medicine, Budapest, Hungary. laszim@drog.sote.hu
Szöke
E
E
Németh
Z
Z
Albert
L
L
D046508:Culture Techniques; D005557:Formaldehyde / Q000378:metabolism*; D008954:Models, Biological; D010944:Plants / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005557:Formaldehyde