Solid-phase extraction method for patulin in apple juice and unfiltered apple juice.
Patulin, a mold metabolite, is commonly found in rotting apples. Some countries regulate patulin at levels ranging from 30 to 50 micrograms/L. Most analytical methods for patulin in apple juice include liquid-liquid partitions. A solid-phase extraction method has been developed for apple juice and unfiltered apple juice in the United States. A portion of the test sample (5 mL) was passed through a macroporous copolymer cartridge and was washed with 1 mL 1% sodium bicarbonate and then with 1 mL 1% acetic acid. Patulin was eluted with 3 mL 2% acetonitrile in anhydrous ethyl ether and was determined by reversed-phase liquid chromatography with UV detection at 276 nm. Recoveries ranged from 93 to 104% in test samples spiked at 20-100 micrograms/L.
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U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Washington, DC 20204, USA.
Tang
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D002853:Chromatography, Liquid; D005374:Filtration; D005506:Food Contamination; D010365:Patulin / Q000032:analysis*; D019685:Rosales / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014481:United States
D010365:Patulin