Microtubule disassembly by ATP-dependent oligomerization of the AAA enzyme katanin.
Katanin, a member of the AAA adenosine triphosphatase (ATPase) superfamily, uses nucleotide hydrolysis energy to sever and disassemble microtubules. Many AAA enzymes disassemble stable protein-protein complexes, but their mechanisms are not well understood. A fluorescence resonance energy transfer assay demonstrated that the p60 subunit of katanin oligomerized in an adenosine triphosphate (ATP)- and microtubule-dependent manner. Oligomerization increased the affinity of katanin for microtubules and stimulated its ATPase activity. After hydrolysis of ATP, microtubule-bound katanin oligomers disassembled microtubules and then dissociated into free katanin monomers. Coupling a nucleotide-dependent oligomerization cycle to the disassembly of a target protein complex may be a general feature of ATP-hydrolyzing AAA domains.
Hartman
J J
JJ
The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA.
Vale
R D
RD
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism*; D000595:Amino Acid Sequence; D002499:Centrifugation, Density Gradient; D005453:Fluorescence; D006868:Hydrolysis; D000074201:Katanin; D008164:Luminescent Proteins; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D011108:Polymers; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D008164:Luminescent Proteins; D011108:Polymers; D011993:Recombinant Fusion Proteins; D014404:Tubulin; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D000074201:Katanin