title: Dietary salt increases endothelial nitric oxide synthase and TGF-beta1 in rat aortic endothelium.
abstract: The amount of NaCl in the diet plays an important role in modulating nitric oxide (NO) synthesis in vivo. In the glomerulus, dietary NaCl also regulates transforming growth factor-beta1 (TGF-beta1) production. We hypothesized that dietary NaCl intake regulated expression of the endothelial isoform of nitric oxide synthase (NOS3) and TGF-beta1 in the aorta. Administration of 8.0% NaCl diet to rats for 7 days did not affect blood pressure but increased steady-state mRNA and protein levels of NOS3 in the arterial wall compared with animals on 0.3% NaCl diet. Northern analysis demonstrated increased steady-state amounts of mRNA of TGF-beta1 in aortas of rats on 8.0% NaCl diet. By ELISA, both total and active TGF-beta1 were increased in these vessel segments. Endothelial denudation of aortic rings reduced active TGF-beta1 secretion to undetectable levels. Addition of a neutralizing antibody to TGF-beta to aortic ring segments attenuated NO production but not to that observed in animals on the 0.3% NaCl diet. The data showed that dietary NaCl intake modulated NOS3 and TGF-beta1 expression in the arterial wall; NOS3 expression was at least partially regulated by endothelial cell production of TGF-beta1.
authors.affiliation: Nephrology Research and Training Center, Comprehensive Cancer Center, Division of Nephrology, Department of Medicine, Birmingham, Alabama 35233, USA.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Ying
authors.forename: P W
authors.initials: PW
authors.lastname: Sanders
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D004357:Drug Synergism; D004730:Endothelium, Vascular / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage* / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000502:physiology
chemical_list: D016212:Transforming Growth Factor beta; D009569:Nitric Oxide; D012965:Sodium Chloride; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat