Leukemia inhibitory factor, glial cell line-derived neurotrophic factor, and their receptor expressions following muscle crush injury.
Using in situ hybridization histochemistry, we characterized the spatiotemporal gene expression patterns of leukemia inhibitory factor (LIF) and glial cell line-derived neurotrophic factor (GDNF), and their receptor components (LIFR, GFR-alpha1, RET) induced in muscle cells, intramuscular nerves, and motoneurons in the regeneration processes of both muscle cells and nerves following muscle contusion. Muscle contusion induced upregulation of GDNF and GFR-alpha1 mRNAs in Schwann cell-like cells in the intramuscular nerves and of LIFR mRNA in damaged muscle cells. LIFR, GFR-alpha1, and RET mRNA expressions in motoneurons were upregulated following muscle contusion. Muscle contusion also induced more rapid, prominent transactivations of GFR-alpha1 and RET genes in motoneurons than did sciatic nerve axotomy. These findings suggest that rapid and prominent upregulation of the receptor components for LIF and GDNF in motoneurons is important for the regeneration of intramuscular motor nerves damaged by muscle contusion.
Kami
K
K
Department of Health Science, Osaka University of Health and Sports Sciences, Noda 1558-1, Kumatori-cho, Sennan-gun, Osaka 590-0496, Japan. kami@ouhs.ac.jp
Morikawa
Y
Y
Kawai
Y
Y
Senba
E
E
D000818:Animals; D019771:Axotomy; D003288:Contusions / Q000235:genetics / Q000503:physiopathology; D003444:Crush Syndrome / Q000235:genetics* / Q000503:physiopathology; D029721:Drosophila Proteins; D019922:GAP-43 Protein / Q000235:genetics; D015870:Gene Expression; D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D006131:Growth Inhibitors / Q000235:genetics*; D017403:In Situ Hybridization; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000235:genetics*; D009046:Motor Neurons / Q000737:chemistry / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000293:injuries* / Q000294:innervation; D009414:Nerve Growth Factors; D009416:Nerve Regeneration / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020800:Receptor, Nerve Growth Factor / Q000235:genetics; D018121:Receptors, Cytokine / Q000235:genetics*; D053606:Receptors, OSM-LIF; D012584:Sciatic Nerve / Q000601:surgery; D013116:Spinal Cord / Q000166:cytology
D029721:Drosophila Proteins; D019922:GAP-43 Protein; C495041:Gdnf protein, rat; C494989:Gfra1 protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507842:Lifr protein, rat; D008222:Lymphokines; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila; C495001:Ret protein, rat