title: Gap junction intercellular communication mediates the competitive cell proliferation disadvantage of irradiated mouse preimplantation embryos in aggregation chimeras.
abstract: Gap junction intercellular communication (GJIC) is thought to play a role in the growth modulation that occurs within cell populations. An example of heterologous growth inhibition (competitive cell proliferation disadvantage) occurs within mouse aggregation chimeras comprised of irradiated and nonirradiated cleavage-stage embryos. The goal of this investigation was to test the hypothesis that GJIC participates in the competitive cell proliferation disadvantage that is expressed by the irradiated embryo in aggregation chimeras. Specifically, we tested the capacity of the GJIC inhibitor 18 alpha-glycyrrhetinic acid (AGA) to inhibit competitive cell proliferation disadvantage in heterologous aggregation chimeras that were comprised of one embryo that was irradiated with 1.0 Gy of (137)Cs gamma rays and then paired with one nonirradiated embryo. We found that AGA successfully inhibited fluorescent dye transfer between irradiated and nonirradiated embryos in heterologous chimeras. Chronic exposure to AGA prevented competitive cell proliferation disadvantage in these radiation chimeras, while exposure to AGA for the first 15 h of culture (prior to gap junction development) did not prevent competitive cell proliferation disadvantage. An unexpected observation was the apparent lack of any effect of inhibiting GJIC by exposure to AGA on blastocyst formation and cell number allocation in the two principal stem cell lineages of the preimplantation mammalian embryo, trophectoderm and inner cell mass.
authors.affiliation: Institute of Toxicology and Environmental Health, Department of Obstetrics and Gynecology, University of California, Davis, California 95616-8615, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Vance
authors.forename: L M
authors.initials: LM
authors.lastname: Wiley
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000528:radiation effects*; D002455:Cell Division / Q000187:drug effects; D002678:Chimera / Q000528:radiation effects*; D004622:Embryo, Mammalian / Q000166:cytology / Q000528:radiation effects*; D047108:Embryonic Development; D005260:Female; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000528:radiation effects*; D006034:Glycyrrhetinic Acid / Q000494:pharmacology; D051379:Mice; D011247:Pregnancy
chemical_list: D005456:Fluorescent Dyes; D006034:Glycyrrhetinic Acid