The developmental fate of green fluorescent mouse embryonic germ cells in chimeric embryos.
Primordial germ cells (PGCs), as precursors of mammalian germ lineage, have been gaining more attention as a new resource of pluripotent stem cells, which bring a great possibility to study developmental events of germ cell in vitro and at animal level. EG4 cells derived from 10.5 days post coitum (dpc) PGCs of 129/svJ strain mouse were established and maintained in an undifferentiated state. With an attempt to study the differentiation capability of EG4 cells with a reporter protein: green fluorescence protein, and the possible application of EG4 cells in the research of germ cell development, we have generated several EG4-GFP cell lines expressing enhanced green fluorescence protein (EGFP) and still maintaining typical characteristics of pluripotent stem cells. Then, the differentiation of EG4-GFP cells in vitro as well as their developmental fate in chimeric embryos which were produced by aggregating EG4-GFP cells to 8-cell stage embryos were studied. The results showed that EG4 cells carrying green fluorescence have a potential use in the research of germ cell development and other related studies.
Xin
X
X
Shanghai Institute of Cell Biology, Chinese Academy of Science.
Yu
Y S
YS
Tsung
H C
HC
Sugano
S
S
Yan
Y C
YC
D000818:Animals; D019070:Cell Lineage; D002678:Chimera; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005854:Germ Cells / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D007422:Intestines / Q000196:embryology; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D010957:Plasmids / Q000378:metabolism; D014162:Transfection
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins