Microstructure and release characteristics of the minipellet, a collagen-based drug delivery system for controlled release of protein drugs.
We have developed the minipellet, a matrix-type system for the sustained delivery of protein drugs using collagen as a biodegradable drug carrier. In this study, we analyzed the microstructure and release profile of the minipellet containing human serum albumin (HSA) as a model drug.The findings suggest that the minipellet has a structure in which collagen fibers are strongly oriented in the direction of extrusion from the nozzle in the molding process of the minipellet, and that HSA exists as fine particulate clusters which are homogeneously distributed among the collagen fibers in the minipellet. During release, the HSA clusters dissolve and HSA is retained within the collagen matrix as a solution.The results of release experiments indicate that HSA release from the minipellet is mainly controlled by diffusion in the collagen matrix, and that sustained release is achieved by the dense structure of the collagen matrix which is formed in the manufacture process. In addition, more detailed study suggests that the minipellet has unique directional release behavior caused by its microstructure.
Maeda
M
M
Formulation Research Laboratories, Sumitomo Pharmaceuticals Co., Ltd., Kurakakiuchi 1-chome, Ibaraki, Osaka, Japan.
Tani
S
S
Sano
A
A
Fujioka
K
K
D003094:Collagen / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000493:pharmacokinetics; D004058:Diffusion; D016503:Drug Delivery Systems / Q000379:methods*; D004343:Drug Implants; D006801:Humans; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D012709:Serum Albumin / Q000493:pharmacokinetics*; D012995:Solubility; D014961:X-Ray Diffraction
D003692:Delayed-Action Preparations; D004343:Drug Implants; D012709:Serum Albumin; D003094:Collagen