Toxic effects of mitomycin-C on cultured corneal keratocytes and endothelial cells.
Improper use of mitomycin-C in ocular medication may result in damage to corneal cells. In this study, the toxic effects of mitomycin-C on cultured porcine keratocytes and endothelial cells were estimated by MTT, 3H-thymidine uptake and cellular counting assay methods. It was found that mitomycin-C caused a dose-dependent toxic effect to keratocytes and endothelial cells. Both cells were treated with mitomycin-C at the concentration ranging from 100, 10, 1, 0.1 to 0.01 microg/ml for 3 min, 5 min or 100 min. The 50% inhibitory dose (ID50) of mitomycin-C to keratocytes and endothelial cells as measured by MTT assay was 0.40, 0.18, 0.16 mg/ml and 0.27, 0.15, 0.14 mg/ml, respectively, after 3, 5 and 100 minutes drug treatment. The ID50 for keratocytes and endothelial cells as measured by 3H-thymidine uptake immediately, 1 day and 7 days after 100 minutes mitomycin-C treatment was 0.3, 0.0002, 143.2 microg/ml and 45.1, 101.1, 450.2 microg/ml, respectively. The ID50 for keratocytes and endothelial cells as measured by cellular counting 1 day and 7 days after mitomycin-C treatment was 232.5, 109.7 microg/ml and 239.9, 367.5 microg/ml, respectively. It is concluded that mitomycin-C is more toxic to cellular proliferation in cultured corneal keratocytes than in endothelial cells.
Wu
K Y
KY
Department of Ophthalmology, Kaohsiung Medical College, Taiwan, Republic of China.
Hong
S J
SJ
Huang
H T
HT
Lin
C P
CP
Chen
C W
CW
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004396:Coloring Agents / Q000493:pharmacokinetics; D003315:Cornea / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004728:Endothelium, Corneal / Q000187:drug effects*; D066298:In Vitro Techniques; D007633:Keratins / Q000187:drug effects*; D016685:Mitomycin / Q000633:toxicity*; D013552:Swine; D013778:Tetrazolium Salts / Q000493:pharmacokinetics; D013844:Thiazoles / Q000493:pharmacokinetics; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D016685:Mitomycin; D007633:Keratins; C022616:thiazolyl blue; D013936:Thymidine