title: Simultaneous determination of metronidazole benzoate, methylparaben, and propylparaben by high-performance liquid chromatography.
abstract: The simultaneous determination of metronidazole benzoate (MB), methylparaben (MP), and propylparaben (PP) in an oral suspension formulation was developed using high-performance liquid chromatography (HPLC). The method was developed using a Novapak C18 (3.9 x 150 mm, 4 microns) column, methanol-water (50:50, v/v) as the mobile phase and an ultraviolet (UV) detector at 254 nm. The peak area response versus concentration was linear in a concentration range from 40 to 400 micrograms/ml of MB, 0.8 to 8.0 micrograms/ml of MP, and 0.2 to 2.0 micrograms of PP. The correlation coefficients were 0.9997 for MB, 0.9987 for MP, and 0.9983 for PP, with relative standard errors of 1.12%, 1.28%, and 1.67%, respectively.
authors.affiliation: Tabuk Pharmaceutical Manufacturing Company, Kingdom of Saudi Arabia.
authors.forename: M S
authors.initials: MS
authors.lastname: Ali
authors.forename: R S
authors.initials: RS
authors.lastname: Chaudhary
authors.forename: M A
authors.initials: MA
authors.lastname: Takieddin
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D001565:Benzoates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004355:Drug Stability; D008795:Metronidazole / Q000032:analysis*; D010226:Parabens / Q000032:analysis*; D012680:Sensitivity and Specificity; D013535:Suspensions
chemical_list: D000890:Anti-Infective Agents; D001565:Benzoates; D004338:Drug Combinations; D010226:Parabens; D013535:Suspensions; D008795:Metronidazole; C015358:methylparaben; C006068:propylparaben