[The effect of oxidative stress on brain nitric oxide synthase activity in vivo and in vitro].
Within 8 days of a 10-min cardiac arrest, accumulation of material reacting with 2-thiobarbituric acid was revealed in the hippocampus (74%) and cerebellum (47%) of male Wistar rats. Oxidative stress was accompanied by a twofold decrease of the nitric oxide synthase activity in the brain tissue. In vitro experiments showed a dose-dependent decrease of nitric oxide synthase activity in the brain homogenates as a result of the oxidative stress induced by hydrogen peroxide or sodium hypochlorite. The findings suggest that the oxidative stress may decrease nitric oxide synthase activity as a result of direct effects of the active oxygen on the enzyme.
Onufriev
M V
MV
Institute of Higher Nervous Activity and Neurophysiology, Russian Acad. Sci., Moscow, Russia.
Stepanichev
M Iu
MIu
Mitrokhina
O S
OS
Moiseeva
Iu V
IuV
Lazareva
N A
NA
Guliaeva
N V
NV
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002545:Brain Ischemia / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric
D005609:Free Radicals; D016877:Oxidants; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D019001:Nitric Oxide Synthase