title: Sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams.
abstract: The sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams was studied under dry conditions. This plasmid contains the supF gene that can be used in mutagenesis analysis. The survival curve of the plasmid had both a shoulder and exponential parts, and showed a D10 value of about 17 kGy for both Escherichia coli wild-type and DNA repair-deficient mutant (UV-sensitive) strains. Therefore, it was assumed that the excision repair system was not effective for repairing DNA lesions induced by irradiation with carbon ion beams.
authors.affiliation: Health Research Foundation, Kyoto-shi, Kyotot, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Ando
authors.forename: S
authors.initials: S
authors.lastname: Sawada
authors.forename: H
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Nagaoka
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: C
authors.initials: C
authors.lastname: Mukai
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D002244:Carbon / Q000009:adverse effects*; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000378:metabolism / Q000528:radiation effects; D005822:Genetic Vectors / Q000378:metabolism / Q000528:radiation effects*; D020450:Heavy Ions / Q000009:adverse effects*; D007477:Ions; D009154:Mutation; D010957:Plasmids / Q000235:genetics* / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects; D014965:X-Rays / Q000009:adverse effects
chemical_list: D007477:Ions; D002244:Carbon