A GC-rich sequence within the 5' untranslated region of human basonuclin mRNA inhibits its translation.
By the method of RNase protection, the 5' ends of the basonuclin mRNA were mapped to four sites distributed over 400 bases of the genomic sequence, a result implying four different basonuclin transcripts within the cell. Despite the heterogeneity at the 5' end, all four basonuclin mRNA shared the same translation initiation codon. However, only two transcripts contained, in their 5' untranslated region (UTR), a GC-rich sequence of approx. 180 bases. The ability of this GC-rich sequence to form a large and stable secondary structure was suggested by experimental results from primer extension, RNase resistance, and computer analysis of the sequence. In vitro study showed that translation of basonuclin RNA containing this putative structure could not be initiated efficiently from the first two AUGs, whereas those that lacked it could.
Tang
W
W
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Tseng
H
H
D020121:5' Untranslated Regions; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins; D020862:GC Rich Sequence; D006801:Humans; D015603:Keratinocytes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010750:Phosphoproteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors
D020121:5' Untranslated Regions; D017931:DNA Primers; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C077914:BNC1 protein, human; D012260:Ribonucleases