Structural features and hypoglycemic activity of a polysaccharide (CS-F10) from the cultured mycelium of Cordyceps sinensis.
A polysaccharide (CS-F10) purified from a hot water extract of the cultured mycelium of Cordyceps sinensis was composed of galactose, glucose and mannose in a molar ratio of 43:33:24; its molecular weight was estimated to be about 15000. The results of chemical and spectroscopic investigations suggest that CS-F10 has a comb-type structure, and has alpha-D-glucopyranosyl residues on the terminal of the side-chains and characteristic sugar residues of C. sinensis i.e., 1,5-linked beta-D-galactofuranosyl residues. CS-F10 significantly lowered the plasma glucose level in normal, streptozotocin (STZ)-induced diabetic and epinephrine-induced hyperglycemic mice after intraperitoneal administration (50 mg/kg). Administration of CS-F10 to STZ-induced diabetic mice significantly increased the activity of hepatic glucokinase. A significant reduction in the hepatic glucose output was observed following the infusion of CS-F10 using the perfused rat liver. CS-F10 also significantly decreased protein content of facilitative glucose transporter isoform 2 (GLUT2) from rat liver following i.p. administration. These effects presumably contribute to the hypoglycemic activity.
Kiho
T
T
Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
Ookubo
K
K
Usui
S
S
Ukai
S
S
Hirano
K
K
D000818:Animals; D003470:Culture Media / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D005941:Glucokinase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2; D006999:Hypocreales / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D003470:Culture Media; D051273:Glucose Transporter Type 2; D007004:Hypoglycemic Agents; D009004:Monosaccharide Transport Proteins; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013311:Streptozocin; D005941:Glucokinase; D005947:Glucose