title: Detection of specific immunoglobulin E during maternal, fetal, and congenital toxoplasmosis.
abstract: Toxoplasma immunoglobulin E (IgE) antibodies in 664 serum samples were evaluated by using an immunocapture method with a suspension of tachyzoites prepared in the laboratory in order to evaluate its usefulness in the diagnosis of acute Toxoplasma gondii infection during pregnancy, congenital infection, and progressive toxoplasmosis. IgE antibodies were never detected in sera from seronegative women, from patients with chronic toxoplasma infection, or from infants without congenital toxoplasmosis. In contrast, they were detected in 86.6% of patients with toxoplasmic seroconversion, and compared with IgA and IgM, the short kinetics of IgE was useful to date the infection precisely. For the diagnosis of congenital toxoplasmosis, specific IgE detected was less frequently than IgM or IgA (25 versus 67.3%), but its detection during follow-up of children may be interesting, reflecting an immunological rebound. Finally, IgE was detected early and persisted longer in progressive toxoplasmosis with cervical adenopathies, so it was also a good marker of the evolution of toxoplasma infection.
authors.affiliation: Laboratory of Parasitology-Mycology, Team 4 (INSERM U.314), EA 2070, IFR 53, Hôpital Maison Blanche, Reims, France. ivillena@chu-reims.fr
authors.forename: I
authors.initials: I
authors.lastname: Villena
authors.forename: D
authors.initials: D
authors.lastname: Aubert
authors.forename: V
authors.initials: V
authors.lastname: Brodard
authors.forename: C
authors.initials: C
authors.lastname: Quereux
authors.forename: B
authors.initials: B
authors.lastname: Leroux
authors.forename: D
authors.initials: D
authors.lastname: Dupouy
authors.forename: G
authors.initials: G
authors.lastname: Remy
authors.forename: F
authors.initials: F
authors.lastname: Foudrinier
authors.forename: C
authors.initials: C
authors.lastname: Chemla
authors.forename: J E
authors.initials: JE
authors.lastname: Gomez-Marin
authors.forename: J M
authors.initials: JM
authors.lastname: Pinon
mesh_terms: D000293:Adolescent; D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood*; D000918:Antibody Specificity; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002825:Chorioretinitis / Q000175:diagnosis / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008875:Middle Aged; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis / Q000276:immunology*; D013997:Time Factors; D014123:Toxoplasmosis / Q000150:complications* / Q000175:diagnosis / Q000276:immunology*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis / Q000276:immunology; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007073:Immunoglobulin E