CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s).
We have recently cloned a putative chemoattractant receptor, named CRTH2, which is preferentially expressed on human T-helper- (Th) 2 but not Th1 cells. In this study, we demonstrated that CRTH2 is also highly expressed on peripheral blood basophils and eosinophils. Our search for a CRTH2 ligand identified mast cells as the possible producers of a ligand. When stimulated with an anti-FcepsilonR1 antibody, cord blood-derived mast cells secreted factor(s) that induced Ca(2+) mobilization in CRTH2-expressing K562 cells but not in mock transfected cells. These findings implied the involvement of CRTH2 in mast cell-mediated immune responses such as allergic reactions.
Nagata
K
K
Research and Development Center, BioMedical Laboratories, Inc., 1361-1 Matoba, Kawagoe, Saitama, Japan. nagata@alk.co.jp
Hirai
H
H
Tanaka
K
K
Ogawa
K
K
Aso
T
T
Sugamura
K
K
Nakamura
M
M
Takano
S
S
D001491:Basophils / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D006967:Hypersensitivity / Q000378:metabolism; D020014:K562 Cells; D008024:Ligands; D008407:Mast Cells / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D011982:Receptors, Prostaglandin; D019089:Stem Cell Factor / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*
D008024:Ligands; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; D019089:Stem Cell Factor; D002118:Calcium; C036293:prostaglandin D2 receptor