Technical manual for manufacturing autologous fibrin tissue adhesive.
OBJECTIVE
The aim of this article is to provide a concise and simple technical manual for manufacturing autologous fibrin tissue adhesive derived from the precipitation of fibrinogen using a combination of ethanol and freezing for surgery.


METHODS
All materials and equipment needed to manufacture ethanol-based autologous fibrin tissue adhesive are listed. In addition, step-by-step instructions are provided to allow for easy and rapid fibrin adhesive production.


RESULTS
Ethanol-based autologous fibrin tissue adhesive can be manufactured in under 60 minutes. Furthermore, at our institution the startup cost for manufacturing ethanol-based autologous fibrin tissue adhesive was under $2,500.00.


CONCLUSIONS
Ethanol-based autologous fibrin tissue adhesive is a safe, reliable, and easily manufactured autologous fibrin tissue adhesive that can be made by a trained technician in any blood bank, pharmacy, or surgical laboratory.
Park
J J
JJ
Department of Surgery, The University of Illinois Hospital and Clinics, Chicago, USA.
Cintron
J R
JR
Siedentop
K H
KH
Orsay
C P
CP
Pearl
R K
RK
Nelson
R L
RL
Abcarian
H
H
D000431:Ethanol; D015718:Fibrin Tissue Adhesive / Q000138:chemical synthesis; D005340:Fibrinogen; D005615:Freezing; D006801:Humans; D014014:Tissue Adhesives / Q000138:chemical synthesis
D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D000431:Ethanol; D005340:Fibrinogen