Hybridization-based karyotyping of mouse chromosomes: hybridization-bands.
We have developed a method, which we have named hybridization-banding, to identify simultaneously all chromosomes in a mouse metaphase spread. The method uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on each chromosome. The method is superior to Giemsa- or fluorescence-based banding methods for chromosome identification because the hybridization patterns are simpler and easier to identify, and unique patterns can be designed at will for each chromosome. Analysis can be performed with a standard fluorescence microscope, and images can be recorded on film with an ordinary 35-mm camera, making the method useful to many investigators. The method can also be applied to any species for which chromosomes and probes can be prepared.
Liechty
M C
MC
Applied Genetics Laboratories, Inc., Melbourne, FL ,USA. Liechty@appliedgenetics.com
Carpio
C M
CM
Aytay
S
S
Clase
A C
AC
Puschus
K L
KL
Sims
K R
KR
Davis
L M
LM
Hozier
J C
JC
D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D015342:DNA Probes; D015723:Gene Library; D016678:Genome; D017403:In Situ Hybridization / Q000379:methods*; D007211:Indoles; D007621:Karyotyping / Q000379:methods*; D008677:Metaphase; D051379:Mice; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity
D015342:DNA Probes; D007211:Indoles; C007293:DAPI