[The notochord proteins of true sturgeons carrying the At1 epitope].
Previously we reported the production of monoclonal antibodies against chordin, an acidic glycoprotein from true sturgeon notochord, carrying glycans terminating with 3-sulfoglucuronic acid. In addition, monoclonal antibodies At1, not reacting with chordin, were produced. Here we describe At1 epitope expression in sturgeon tissues and target proteins for At1 antibodies, and test interaction of these proteins with chordin and other molecules carrying glycan with 3-sulfoglucuronic acid. The expression of the antigens carrying At1 epitope during sturgeon development has also been studied.
Preobrazhenskiĭ
A A
AA
Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Barabanov
V M
VM
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D000939:Epitopes / Q000276:immunology*; D005399:Fishes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D009672:Notochord / Q000276:immunology*; D011506:Proteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D011506:Proteins; C054234:chordin