Production, purification and characterization of thermophilic lipase from Bacillus sp. THL027.
A low-cost medium, MGRS, has been developed for growth and lipase production from Bacillus THL027 at 65 degrees C and pH 7.0. MGRS was composed of 2% (v/v) buffer solution (7.3% (w/v) Na(2)HPO(4), 3.2% (w/v) KH(2)PO(4), pH 7.2), 40 microg ml(-1) FeSO(4) and 40 microg ml(-1) MgSO(4), 0.1% (w/v) (NH(4))(2)SO(4) supplemented with 3% NaCl, 0.1% glucose, 1.0% rice bran oil and 0.5% (w/v) rice bran. The lipase was purified 2.6-fold to apparent homogeneity by ultrafiltration and gel filtration chromatography. Its molecular mass was 69 kDa. The purified enzyme was characterized for its general physical properties.
Dharmsthiti
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S
Center for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Bhuddhamonthon 4 Rd, Salaya, Nakornprathom, Thailand.stsds@mahidol.ac.th
Luchai
S
S
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media; D008049:Lipase