Expression of cell-homologous genes of human herpesvirus-8 in human immunodeficiency virus-negative lymphoproliferative diseases.
Human herpesvirus-8 (HHV-8) genome encodes for genes homologous to human cellular genes such as interleukin-6 (IL-6), Cyclin-D, BCL-2, and IL-8 receptor (G-protein-coupled receptor [GCR]). We used reverse transcriptase-polymerase chain reaction to study the expression of these viral genes in lymphoproliferative disorders associated with HHV-8 infection. None of these genes was expressed in 1 case of benign, localized Castleman's disease (CD), and only viral IL-6 and viral Cyclin-D were transcribed in 2 cases of benign lymphadenopathies with giant germinal center hyperplasia and increased vascularity. In contrast, all 4 genes were transcribed in 1 case of multicentric CD of plasma cell type with aggressive clinical course and in 1 primary effusion lymphoma cell line. Our study provides the evidence that various HHV-8 genes, homologous to cellular genes involved in control of proliferation and apoptosis, may be differently expressed in different lymphoid disorders in vivo.
Luppi
M
M
Department of Medical Sciences, Section of Hematology, Modena, Italy.
Barozzi
P
P
Maiorana
A
A
Trovato
R
R
Marasca
R
R
Morselli
M
M
Cagossi
K
K
Torelli
G
G
D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D017209:Apoptosis; D005871:Castleman Disease / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D002455:Cell Division; D056741:Cyclin D; D016213:Cyclins / Q000096:biosynthesis / Q000235:genetics; D019254:Genes, bcl-2; D006678:HIV / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008206:Lymphatic Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008221:Lymphoid Tissue / Q000378:metabolism* / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics; D023062:Receptors, Interleukin-8A; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D017735:Virus Latency
D015703:Antigens, CD; D056741:Cyclin D; D016213:Cyclins; D015850:Interleukin-6; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D014764:Viral Proteins