Regulation of sulphotransferase expression in the endometrium during the menstrual cycle, by oral contraceptives and during early pregnancy.
The endometrium plays a key role in reproduction, and this function is tightly regulated by endogenous and xenobiotic steroids. Sulphation, catalysed by members of the sulphotransferase (SULT) enzyme family, is a major deactivating mechanism for steroid hormones and we have investigated the expression and regulation in vivo of SULT in the human endometrium. In the normal cycling endometrium, expression of the phenol sulphotransferases SULT1A1 and SULT1A3 and the oestrogen sulphotransferase SULT1E1 were observed, with SULT1A1 and SULT1E1 expression being higher in the luteal phase than in the follicular phase. No expression of the hydroxysteroid sulphotransferase SULT2A1 was detected at any time in the endometrium. In endometrium from women taking the combined oral contraceptive pill (OCP), SULT1E1 expression was virtually absent, and SULT1A1 expression was substantially reduced. Similarly, in early pregnancy (i.e. first trimester) endometrium, SULT1E1 expression was absent, although SULT1A1 and SULT1A3 expression were unaffected. Our results with normal endometrium support in-vitro data showing that SULT1E1 expression is regulated by progesterone. However, the data obtained from OCP and early pregnancy endometrium suggest that factors other than the concentration of circulating progesterone are involved in the regulation of the expression of this important enzyme in the endometrium.
Rubin
G L
GL
Department of Molecular & Cellular Pathology, University of Dundee, Dundee DD1 9SY, UK.
Harrold
A J
AJ
Mills
J A
JA
Falany
C N
CN
Coughtrie
M W
MW
D000328:Adult; D003276:Contraceptives, Oral / Q000494:pharmacology*; D004347:Drug Interactions; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008597:Menstrual Cycle / Q000235:genetics*; D008875:Middle Aged; D011247:Pregnancy; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics*
D003276:Contraceptives, Oral; D015238:Sulfotransferases