Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252.
A strain of Acinetobacter baumannii cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase (ppk) gene from the strain. The promoter for the ppk gene was functional in the heterologous Escherichia coli host. Using RT-PCR, transcription of the ppk gene was found to be regulated by phosphate concentration.
Gavigan
J A
JA
Microbiology Department and National Food Biotechnology Centre, National University of Ireland Cork.
Marshall
L M
LM
Dobson
A D
AD
D020121:5' Untranslated Regions / Q000235:genetics; D000150:Acinetobacter / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010710:Phosphates; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase