Multidrug resistance and retroviral transduction potential in human small cell lung cancer cell lines.
Multidrug resistance (MDR) remains a major problem in the successful treatment of small cell lung cancer (SCLC). New treatment strategies are needed, such as gene therapy specifically targeting the MDR cells in the tumor. Retroviral LacZ gene-containing vectors that were either pseudotyped for the gibbon ape leukemia virus (GALV-1) receptor or had specificity for the amphotropic murine leukemia virus (MLV-A) receptor were used for transduction of five SCLC cell lines differing by a range of MDR mechanisms. Transduction efficiencies in these cell lines were compared by calculating the percentage of blue colonies after X-Gal staining of the cells grown in soft agar. All examined SCLC cell lines were transducible with either vector. Transduction efficiencies varied from 5.7% to 33.5% independent of the presence of MDR. These results indicate that MDR does not severely impair transduction of SCLC cells, and that MLV-A as well as GALV-1 retroviral vectors are suitable for further development of gene therapy in SCLC.
Theilade
M D
MD
Laboratory for Infectious Diseases, Department 144, Hvidovre Hospital, Denmark.
Gram
G J
GJ
Jensen
P B
PB
Cianfriglia
M
M
Rørth
M
M
Hansen
J E
JE
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis; D018288:Carcinoma, Small Cell / Q000628:therapy*; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000378:metabolism; D018432:Drug Resistance, Multiple; D005434:Flow Cytometry; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000628:therapy*; D008856:Microscopy, Fluorescence; D011991:Receptors, Virus / Q000235:genetics; D012190:Retroviridae; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D011991:Receptors, Virus; C067981:leukemia virus receptor, gibbon ape