Localization of L-glutamate and glutamate-like receptors at the squid giant synapse.
HPLC analysis of the amino acid contents of the second- and third-order giant fibres at the giant synapse in the stellate ganglion of the squid Loligo vulgaris shows that there are significantly higher amounts of L-glutamate and L-aspartate in the second-order (presynaptic) fibre than in the third-order (postsynaptic) fibre. Immunocytochemical staining of sections of the ganglion with an antibody raised against L-glutamate produces specific positive staining in the synaptic region of the second-order fibre. In contrast, staining with antibodies raised against glutamate-receptors (mammalian GluR1 with GluR2/3) produces positive staining in the third-order fibre at the postsynaptic region. These data provide further evidence for the hypothesis that L-glutamate is an excitatory transmitter at the giant synapse.
Di Cosmo
A
A
Dipartimento di Zoologia, Università degli Studi Federico II, Naples, Italy.
Nardi
G
G
Di Cristo
C
C
De Santis
A
A
Messenger
J B
JB
D000409:Alanine / Q000032:analysis / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D001224:Aspartic Acid / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D049832:Decapodiformes / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D017952:Ganglia, Invertebrate / Q000737:chemistry; D018698:Glutamic Acid / Q000032:analysis* / Q000276:immunology; D005998:Glycine / Q000032:analysis / Q000276:immunology; D017470:Receptors, Glutamate / Q000032:analysis* / Q000276:immunology; D012694:Serine / Q000032:analysis / Q000276:immunology; D013569:Synapses / Q000737:chemistry*; D013654:Taurine / Q000032:analysis / Q000276:immunology; D005680:gamma-Aminobutyric Acid / Q000032:analysis / Q000276:immunology
D017470:Receptors, Glutamate; D013654:Taurine; D001224:Aspartic Acid; D018698:Glutamic Acid; D012694:Serine; D005680:gamma-Aminobutyric Acid; D000409:Alanine; D005998:Glycine