Phagocytic activity of ethyl alcohol fraction of deer antler in murine peritoneal macrophage.
The mechanism of phagocytic activity of the ethyl alcohol fraction of Cervus nippon (CN-E) was investigated in vivo. The administration of CN-E (100 mg/kg, p.o.) enhanced lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles in murine peritoneal macrophages. Phagocytic activity was suppressed by the treatment of S-nitrosoglutathione (GSNO) which is an exogenous nitric oxide donor depending on the concentration of dose. CN-E suppressed the production of nitric oxide and enhanced the concentration in [Ca2+]i. The enhancement in [Ca2+]i was diminished by the treatment of EGTA. These results indicate that CN-E enhances the phagocytic activity of murine peritoneal macrophage via a suppression of nitric oxide production and an increase in [Ca2+]i.
Suh
J S
JS
Department of Chemistry, Center for Cell Signaling Research, Ewha Womans University, Seoul, Korea.
Eun
J S
JS
So
J N
JN
Seo
J T
JT
Jhon
G J
GJ
D000818:Animals; D000999:Antlers / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D003670:Deer; D000431:Ethanol / Q000737:chemistry; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts; D009569:Nitric Oxide; D000431:Ethanol; D002118:Calcium