title: Caveolin-1 modulates the activity of the volume-regulated chloride channel.
abstract: 1. Caveolae are small invaginations of the plasma membrane that have recently been implicated in signal transduction. In the present study, we have investigated whether caveolins, the principal protein of caveolae, also modulate volume-regulated anion channels (VRACs). 2. ICl,swell, the cell swelling-induced chloride current through VRACs, was studied in three caveolin-1-deficient cell lines: Caco-2, MCF-7 and T47D. 3. Electrophysiological measurements showed that ICl, swell was very small in these cells and that transient expression of caveolin-1 restored ICl,swell. The caveolin-1 effect was isoform specific: caveolin-1beta but not caveolin-1alpha upregulated VRACs. This correlated with a different subcellular distribution of caveolin-1alpha (perinuclear location) from caveolin-1beta (perinuclear and peripheral). 4. To explain the modulation of ICl, swell by caveolin-1 we propose that caveolin increases the availability of VRACs in the plasma membrane or, alternatively, that it plays a crucial role in the signal transduction cascade of VRACs.
authors.affiliation: Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Trouet
authors.forename: B
authors.initials: B
authors.lastname: Nilius
authors.forename: A
authors.initials: A
authors.lastname: Jacobs
authors.forename: C
authors.initials: C
authors.lastname: Remacle
authors.forename: G
authors.initials: G
authors.lastname: Droogmans
authors.forename: J
authors.initials: J
authors.lastname: Eggermont
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018938:Caco-2 Cells; D002417:Cattle; D051242:Caveolin 1; D022461:Caveolins; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D048429:Cell Size / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology*; D004594:Electrophysiology; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D007536:Isomerism; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D018613:Microscopy, Confocal; D018408:Patch-Clamp Techniques; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection / Q000502:physiology
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D018118:Chloride Channels; D008565:Membrane Proteins