title: Modulation of B-lymphocyte and NK cell activities by glycoinositolphospholipid purified from Trypanosoma cruzi.
abstract: Glycoinositolphospholipids (GIPLs) are some of the major glycolipids of the Trypanosoma cruzi surface that were previously shown to activate B cells. In the present study, we investigated whether (i) T. cruzi GIPLs could induce immunoglobulin secretion from B cells in the absence of T cells and NK cells and whether (ii) NK cells are also stimulated by the GIPLs. B cells purified from mice deficient in both T and NK cells (CD3epsilon transgenic mice) secreted immunoglobulin in response to the GIPL. This response was increased by coculture with a murine NK cell line. The T. cruzi GIPL also increased the NK cell (interleukin-2 induced) proliferative response. Our data indicate that the T. cruzi GIPL has a direct stimulatory effect on NK cells and induces immunoglobulin secretion in the absence of T lymphocytes and NK cells. These findings suggest that this T. cruzi-derived molecule may be one of the stimulators that lead to NK cell activation during T. cruzi infection.
authors.affiliation: Department of Immunology, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: L B
authors.initials: LB
authors.lastname: De Arruda Hinds
authors.forename: L M
authors.initials: LM
authors.lastname: Previato
authors.forename: J O
authors.initials: JO
authors.lastname: Previato
authors.forename: Q
authors.initials: Q
authors.lastname: Vos
authors.forename: J J
authors.initials: JJ
authors.lastname: Mond
authors.forename: L M
authors.initials: LM
authors.lastname: Peçanha
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D006001:Glycoconjugates / Q000494:pharmacology*; D007075:Immunoglobulin M / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D010716:Phosphatidylinositols / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D006001:Glycoconjugates; D007075:Immunoglobulin M; D007376:Interleukin-2; D010716:Phosphatidylinositols