Immunogenicity of alpha-toxin, capsular polysaccharide (CPS) and recombinant fibronectin-binding protein (r-FnBP) of Staphylococcus aureus in rabbit.
This study was conducted to evaluate the antibody levels of alpha-toxin, capsular polysaccharides (CPS) and fibronectin-binding protein (FnBP) in rabbits immunized with an experimental vaccine against Staphylococcus aureus and to develop the bovine mastitis subunit vaccine in the future. Enzyme immunoassay was used for detection of IgG antibodies against staphylococcal CPS, alpha-toxin and FnBP. The levels of specific antibodies against CPS, alpha-toxin and FnBP in immunized rabbits were significantly increased after first immunization compared with control animals (p<0.05). Of three antigen used in vaccine, immunogenicity of CPS was relatively lower, compared with those of alpha toxin and fibronectin binding protein. Numbers of S. aureus in blood of immunized groups were lower than those of control group after bacterial challenge. But the bacterial numbers among immunized groups were not significantly different. S. aureus counts in excised organs were significantly lower in all immunized rabbits than in PBS-control group (p<0.05). The present study showed that alpha-toxin, capsular polysaccharide and fibronectin binding protein included in a subunit vaccine were protective.
Park
H M
HM
Department of Internal Medicine and Infectious Disease, College of Veterinary Medicine, Seoul National University, Sinlim-dong, Korea.
Yoo
H S
HS
Oh
T H
TH
Kim
D
D
Han
H R
HR
D018829:Adhesins, Bacterial; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D016667:Bacterial Capsules / Q000097:blood / Q000276:immunology*; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D002352:Carrier Proteins / Q000097:blood / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D015169:Colony Count, Microbial / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007273:Injections, Intramuscular / Q000662:veterinary; D008099:Liver / Q000382:microbiology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity; D010738:Type C Phospholipases / Q000097:blood / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D011994:Recombinant Proteins; C054106:capsular polysaccharide, type 5, Staphylococcus aureus; C078635:fibronectin-binding proteins, bacterial; D010738:Type C Phospholipases