title: Cytotoxic evaluation of root-end filling materials in cultures of human osteoblast-like cells and periodontal ligament cells.
abstract: The cytotoxicity of three root-end filling materials (amalgam, IRM, and Super-EBA) was evaluated in cultures of human periodontal ligament cells and human osteoblast-like cells. Ten-millimeter-long plastic test tubes were filled with 3 mm of freshly mixed root-end filling materials at one end (1.5 mm diameter). The opposite end was sealed and attached by heat to a 35-mm cell culture dish. Human periodontal ligament cells and human osteoblast-like cells were seeded in the dishes. The size of cell-free zones around the root-end filling materials and the total cell number per dish were calculated after 3 and 7 days. Empty test tubes used as controls did not influence the growth and distribution of the cultured cells. Cell density increased in all groups in the test period. Amalgam had a larger cell-free zone, compared with IRM and Super-EBA and showed a reduction in total cell number per dish for both tested cell types. IRM and Super-EBA also had a cell-free inhibition zone for both cell types, but no significant reduction in total cell number per dish. This study showed that amalgam had a higher cell toxicity to human periodontal ligament cells and human osteoblast-like cells than IRM and Super-EBA.
authors.affiliation: Department of Restorative Dentistry and Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030-1715, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: K E
authors.initials: KE
authors.lastname: Safavi
authors.forename: L S
authors.initials: LS
authors.lastname: Spangberg
mesh_terms: D000704:Analysis of Variance; D002478:Cells, Cultured; D003723:Dental Amalgam / Q000633:toxicity; D017220:Dentin-Bonding Agents / Q000633:toxicity; D006801:Humans; D008768:Methylmethacrylates / Q000633:toxicity; D010006:Osteoblasts / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000633:toxicity*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D017220:Dentin-Bonding Agents; D008768:Methylmethacrylates; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C031667:IRM cement; D003723:Dental Amalgam; C084074:Super EBA