title: A role for the novel cytokine RANTES in pregnancy and parturition.
abstract: OBJECTIVE
  RANTES (regulated on activation, normal T cell expressed and secreted), a potent and versatile chemokine, is capable of attracting monocytes, lymphocytes, basophils, and eosinophils. This cytokine has been implicated in the regulation of the inflammatory response and in the recruitment of macrophages to the implantation site in early pregnancy. RANTES messenger ribonucleic acid and protein have been detected in fetal tissue and first-trimester trophoblast in response to bacterial endotoxin. The purpose of this study was to determine whether intrauterine infection, parturition (preterm and term), and gestational age affect the amniotic fluid concentrations of RANTES in human pregnancy.
  
  
  METHODS
  A cross-sectional study was designed to examine the relationship between labor, microbial invasion of the amniotic cavity, gestational age, and RANTES expression in amniotic fluid. Amniotic fluid was obtained from 214 women in the following groups: (1) midtrimester (n = 22), (2) preterm labor with intact membranes in the presence (n = 20) or absence (n = 74) of microbial invasion of the amniotic cavity, (3) term, not in labor (n = 44) and term, in labor in the presence (n = 27) and absence (n = 27) of microbial invasion of the amniotic cavity. Microbial invasion of the amniotic cavity was defined as a positive amniotic fluid culture for microorganisms. RANTES concentrations were determined by use of a sensitive and specific immunoassay.
  
  
  RESULTS
  (1) Amniotic fluid RANTES concentrations decrease with advancing gestational age (r = 0. 43; P <.01). (2) Labor at term was associated with an increase in median concentrations of RANTES (labor-median, 8.4 pg/mL; range, <1.3-94.4 vs no labor-median, <1.3 pg/mL; range, <1.3-230.3; P <.01). (3) Women with preterm labor who delivered preterm (no microbial invasion of the amniotic cavity) had a higher median concentration of amniotic fluid RANTES than those who delivered at term (median, 12.7 pg/mL; range, <1.3-928 vs median, <1.3 pg/mL; range, <1.3-127. 5; P <.001). (4) Microbial invasion of the amniotic cavity was associated with a significant increase in median amniotic fluid RANTES in both preterm and term labor (preterm labor with microbial invasion of the amniotic cavity-median, 51.6 pg/mL; range, <1.3-2290 vs preterm labor without microbial invasion of the amniotic cavity-median, 12.7 pg/mL; range, <1.3-928 and vs preterm labor with delivery at term-median, <1.3 pg/mL; range, <1.3-127.5; P <.001 for each; term labor with microbial invasion of the amniotic cavity-median, 16.8 pg/mL; range, <1.3-171.4 vs term labor without microbial invasion of the amniotic cavity-median, 8.4 pg/mL; range, <1.3-94.4; P <.05 and vs no labor and no microbial invasion of the amniotic cavity-median, 1.4 pg/mL; range, <1.3-230.3; P <.001 and P <.05, respectively).
  
  
  CONCLUSIONS
  These results support a role for RANTES in the mechanisms of human parturition and in the regulation of the host response to intrauterine infection.
authors.affiliation: Perinatology Research Branch, National Institute of Child Health and Human Development, Bethesda, MD, USA.
authors.forename: N
authors.initials: N
authors.lastname: Athayde
authors.forename: R
authors.initials: R
authors.lastname: Romero
authors.forename: E
authors.initials: E
authors.lastname: Maymon
authors.forename: R
authors.initials: R
authors.lastname: Gomez
authors.forename: P
authors.initials: P
authors.lastname: Pacora
authors.forename: H
authors.initials: H
authors.lastname: Araneda
authors.forename: B H
authors.initials: BH
authors.lastname: Yoon
mesh_terms: D000650:Amnion / Q000382:microbiology; D000653:Amniotic Fluid / Q000378:metabolism; D001419:Bacteria / Q000302:isolation & purification; D018946:Chemokine CCL5 / Q000032:analysis / Q000502:physiology*; D003430:Cross-Sectional Studies; D005260:Female; D005865:Gestational Age; D006801:Humans; D007118:Immunoassay; D007743:Labor, Obstetric / Q000502:physiology*; D007752:Obstetric Labor, Premature / Q000378:metabolism; D011247:Pregnancy / Q000502:physiology*
chemical_list: D018946:Chemokine CCL5