Optimized production of recombinant bluetongue core-like particles produced by the baculovirus expression system.
The baculovirus-expression vector system (BEVS) has been widely used for the experimental production of many human and animal single- and multi-unit vaccines, heterologous proteins, and viral insecticides. In this work, the production of recombinant bluetongue virus core-like particles (CLPs), using Sf9 cells in shaker-suspension culture with the SF900 II medium (GIBCO, NY), has been studied. This system involved the simultaneous production of two proteins, VP7 and VP3, and was shown to achieve high volumetric productivities. The key parameters of the time of infection (TOI), and the multiplicity of infection (MOI) were studied. The results show that the peak-volumetric yields and cell-specific yields achieved using low MOIs at low-cell densities were the same as those obtained following infections with a high MOI at high-cell densities. This work establishes the feasibility of using low MOIs in the baculovirus system to produce complex multiprotein particles.
Zheng
Y Z
YZ
Department of Chemical Engineering, The University of Queensland, QLD, 4072, Australia. yuanZ@qimr.edu.au
Greenfield
P F
PF
Reid
S
S
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001709:Biotechnology; D001820:Bluetongue virus / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D018411:Spodoptera; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics
D011994:Recombinant Proteins; C074954:VP3 protein, Bluetongue virus; D014758:Viral Core Proteins; D014765:Viral Vaccines; C066862:VP7 protein, orbivirus