title: Artefactual pyruvate and 2-oxobutyrate produced by trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of oxygen.
abstract: Trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of headspace atmospheric oxygen is shown to produce the trimethylsilyl derivatives of pyruvic and 2-oxobutyric acids, along with 2-hydroxy-2-methylmalonic and 2-hydroxy-2-ethylmalonic acids, respectively. This may lead to overestimation of these keto acids, if they were not oximated in the original sample, and the mistaken reporting of the 2-hydroxymalonates.
authors.affiliation: Biomedical Mass Spectrometry Unit, McGill University, Montréal, Québec, Canada.
authors.forename: O A
authors.initials: OA
authors.lastname: Mamer
authors.forename: L
authors.initials: L
authors.lastname: Choiniere
authors.forename: D
authors.initials: D
authors.lastname: Boismenu
authors.forename: F
authors.initials: F
authors.lastname: Lepine
mesh_terms: D016477:Artifacts; D002087:Butyrates / Q000378:metabolism*; D008314:Malonates / Q000378:metabolism*; D008764:Methylmalonic Acid / Q000378:metabolism*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D014297:Trimethylsilyl Compounds / Q000378:metabolism
chemical_list: D002087:Butyrates; D008314:Malonates; D011773:Pyruvates; D014297:Trimethylsilyl Compounds; C038080:ethylmalonic acid; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; C005087:alpha-ketobutyric acid; D008764:Methylmalonic Acid; D010100:Oxygen