Characterization of the Coxiella burnetti sucB gene encoding an immunogenic dihydrolipoamide succinyltransferase.
The Coxiella burnetii sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme was cloned by immunological screening of a lambda EMBL3 genomic library prepared from strain Nine Mile DNA and sequenced. The homology of the cloned gene product to the counterpart in Escherichia coli was 54.3%, but the homology of the N-terminal region was only 42%. The gene was expressed in E. coli as an independent unit from its own promoter, producing an immunoreactive protein of about 50 kDa on SDS-PAGE which reacted with antisera from laboratory animals and sera from human patients with acute Q fever. The study results suggest that the C. burnetii E2o enzyme may serve as a potential target antigen for diagnostic assays for Q fever.
Nguyen
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SV
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
To
H
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Yamaguchi
T
T
Fukushi
H
H
Hirai
K
K
D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D016997:Coxiella burnetii / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011778:Q Fever / Q000097:blood / Q000276:immunology; D011817:Rabbits; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D000217:Acyltransferases; C021515:dihydrolipoamide succinyltransferase