Serum albumin-alginate coated beads: mechanical properties and stability.
According to a previously described method, alginate beads were prepared from a Na-alginate solution containing propylene glycol alginate (PGA) and human serum albumin (HSA). The solution was added dropwise to a CaCl2 solution. The beads were treated with NaOH, which started the formation of amide bonds between HSA and PGA at the periphery, giving a membrane. Batches of beads with increasingly thick membranes were prepared using growing concentrations of NaOH, and studied with a texture analyser. When raising NaOH concentration, the rupture strength progressively increased, and the resistance strength to a deformation of 50% of total height also increased before slightly decreasing for the highest NaOH concentration. Variations of bead elasticity were also observed. When the beads were prepared with saline reducing gelation time from 10 to 5 min, and reaction time from 15 to 5 min, mechanical properties varied more progressively with the NaOH concentration, while the results became more reproducible. A series of assays conducted with 0.01 M NaOH confirmed the importance of using a short gelation time, and saline rather than water. Stability assays were also performed. The results were compared to those of alginate-polylysine coated beads and showed the interest of the transacylation method.
Edwards-Lévy
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Laboratoire de Pharmacotechnie, UPRES-A CNRS 6013, Faculté de Pharmacie, Université de Reims, France.
Lévy
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MC
D000464:Alginates / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D019245:Compressive Strength; D004355:Drug Stability; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D008863:Microspheres; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D013314:Stress, Mechanical; D013997:Time Factors
D000464:Alginates; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D012709:Serum Albumin; C051546:alginate-polylysine-alginate; D011107:Polylysine; D012965:Sodium Chloride; D012972:Sodium Hydroxide; D020723:Glucuronic Acid