Characterization of outer arm dynein in sea anemone, Anthopleura midori.
Outer arm dynein was purified from sperm flagella of a sea anemone, Anthopleura midori, and its biochemical and biophysical properties were characterized. The dynein, obtained at a 20S ATPase peak by sucrose density gradient centrifugation, consisted of two heavy chains, three intermediate chains, and seven light chains. The specific ATPase activity of dynein was 1.3 micromol Pi/mg/min. Four polypeptides (296, 296, 225, and 206 kDa) were formed by UV cleavage at 365 nm of dynein in the presence of vanadate and ATP. In addition, negatively stained images of dynein molecules and the hook-shaped image of the outer arm of the flagella indicated that sea anemone outer arm dynein is two-headed. In contrast to protist dyneins, which are three-headed, outer arm dyneins of flagella and cilia in multicellular animals are two-headed molecules corresponding to the two heavy chains. Phylogenetic considerations were made concerning the diversity of outer arm dyneins.
Mohri
H
H
National Institute for Basic Biology, Okazaki, Aichi, Japan. mohri@nibb.ac.jp
Inaba
K
K
Kubo-Irie
M
M
Takai
H
H
Yano-Toyoshima
Y
Y
D000818:Animals; D004398:Dyneins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D010802:Phylogeny; D012615:Sea Anemones / Q000145:classification / Q000201:enzymology*; D013082:Sperm Tail / Q000648:ultrastructure
D004398:Dyneins