title: Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion.
abstract: To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17beta-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta were incubated in duplicates in the presence or absence of cytokines or 17beta-oestradiol. Medium was harvested on day 2 and the culture stopped on day 4. Results were corrected for protein content of each individual well and expressed as percent of controls per day (mean +/- SEM). Basal CTB leptin production was 25.2 +/- 2.6 (ng/mg prot). In comparison with controls, leptin production was stimulated to 320 +/- 16% (P < 0.0001) and 195 +/- 3.2% (P < 0.0004) by 3 and 10 ng/ml of interleukin-1alpha respectively. 17beta-oestradiol 10(-6) to 10(-9) mol/l increased basal leptin production 5-9-fold, while 10(-5) mol/l had no such effect. Basal CTB HCG secretion was 5722 +/- 1055 (mIU/mg prot). There was a dose-dependent leptin-induced increase in HCG secretion (P = 0.0039) reaching a 5-fold increase with a leptin concentration of 1 microg/ml (P < 0.006). Gonadotrophin-releasing hormone (GnRH) 8.5 x 10(-8) mol/l significantly increased HCG secretion to 140 +/- 21% of controls (P = 0.031). Cetrorelix (0.1 microg/ml) inhibited leptin-induced HCG secretion (P = 0.0028).
authors.affiliation: Department of Obstetrics and Gynecology, Clinic of Infertility and Gynecological Endocrinology - WHO Collaborating Center in Human Reproduction, University Hospital of Geneva, 32 Bvd de la Cluse, 1211 Geneva, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Chardonnens
authors.forename: P
authors.initials: P
authors.lastname: Cameo
authors.forename: M L
authors.initials: ML
authors.lastname: Aubert
authors.forename: F P
authors.initials: FP
authors.lastname: Pralong
authors.forename: D
authors.initials: D
authors.lastname: Islami
authors.forename: A
authors.initials: A
authors.lastname: Campana
authors.forename: R C
authors.initials: RC
authors.lastname: Gaillard
authors.forename: P
authors.initials: P
authors.lastname: Bischof
mesh_terms: D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D007375:Interleukin-1; D020738:Leptin; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol