Introduction of chromosome 7 suppresses telomerase with shortening of telomeres in a human mesothelial cell line.
Introduction of human chromosome 7 by microcell-mediated chromosome transfer induced senescence in a telomerase-positive human mesothelial cell line, MeT5A. In microcell hybrids which underwent senescence, telomerase activity was decreased before entering senescence and telomeric sequences were shortened as cell division proceeded. Concomitantly, expression of the gene encoding telomerase catalytic subunit was abolished, whereas the genes encoding the RNA component of telomerase and its associated protein TEP1 were not affected. In revertants which arose from such microcell hybrids, telomerase activity was restored and the telomeric sequences were elongated. In microcell hybrids which showed no growth arrest, telomerase activity was unaltered. These results suggest that a putative mortality gene on chromosome 7 negatively regulates the telomere maintenance mechanism in MeT5A.
Nakabayashi
K
K
Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, Totsuka-ku, Maioka-cho 641-12, Yokohama, 244-0813, Japan.
Ogino
H
H
Michishita
E
E
Satoh
N
N
Ayusawa
D
D
D002897:Chromosomes, Human, Pair 7; D004848:Epithelium; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006801:Humans; D019098:Telomerase / Q000235:genetics*; D016615:Telomere / Q000235:genetics* / Q000648:ultrastructure
D019098:Telomerase