Characterization of a Sarcocystis neurona isolate (SN6) from a naturally infected horse from Oregon.
An isolate of Sarcocystis neurona (SN6) was obtained from the spinal cord of a horse from Oregon with neurologic signs. The parasite was isolated in cultures of bovine monocytes and equine spleen cells. The parasite divided by endopolygeny and completed at least one asexual cycle in cell cultures in three days. Two gamma interferon knockout mice inoculated with cell culture-derived merozoites became ill 35 d later and S. neurona schizonts and merozoites were found in encephalitic lesions. The parasite in tissue sections of mice reacted with S. neurona-specific antibodies and S. neurona was reisolated from the brain of knockout mice.
Dubey
J P
JP
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Maryland 20705-2350, USA. Jdubey@lpsi.barc.usda.gov
Mattson
D E
DE
Speer
C A
CA
Baker
R J
RJ
Mulrooney
D M
DM
Tornquist
S J
SJ
Hamir
A N
AN
Gerros
T C
TC
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004679:Encephalomyelitis / Q000469:parasitology / Q000662:veterinary*; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses / Q000469:parasitology; D015151:Immunoblotting; D016867:Immunocompromised Host; D008297:Male; D051379:Mice; D009922:Oregon; D011817:Rabbits; D012522:Sarcocystis / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D012523:Sarcocystosis / Q000469:parasitology / Q000662:veterinary*; D013116:Spinal Cord / Q000469:parasitology*