title: Oral contraceptives that contain ethinyl estradiol (0.035 mg) and cyproterone acetate (2 mg) inhibit leukocyte transmigration through endothelial cell monolayers.
abstract: OBJECTIVE
  To investigate the influence of ethinyl estradiol and cyproterone acetate in oral contraceptives on leukocyte migration through endothelial cell monolayers.
  
  
  METHODS
  Experimental in vitro prospective study.
  
  
  METHODS
  An academic research laboratory.
  
  
  METHODS
  Endothelial cells were cultured on microporous membranes to produce monolayers. Polymorphonuclear leukocytes were used in a previously described migration assay (n = 7). The amount of untreated polymorphonuclear leukocytes that migrated through untreated endothelial cell monolayers was used as a control and set at 100%. In addition, a leukocyte adhesion assay was used.
  
  
  METHODS
  Leukocyte adhesion to and transmigration through endothelial cell monolayers.
  
  
  RESULTS
  Ethinyl estradiol and cyproterone acetate inhibited the migration of polymorphonuclear leukocytes through endothelial cell monolayers significantly (67% +/- 6.4%) when both cell types were treated to simulate in vivo conditions. The adhesion assay produced similar results.
  
  
  CONCLUSIONS
  Ethinyl estradiol and cyproterone acetate were identified as potent inhibitors of leukocyte migration through endothelial cell monolayers.
authors.affiliation: Department of Medical and Chemical Laboratory Diagnostics, University of Vienna, Wilhelminenspital, Austria. roland.hofbauer@akh-wien.ac.at
authors.forename: R
authors.initials: R
authors.lastname: Hofbauer
authors.forename: M
authors.initials: M
authors.lastname: Frass
authors.forename: B
authors.initials: B
authors.lastname: Gmeiner
authors.forename: H
authors.initials: H
authors.lastname: Salfinger
authors.forename: H
authors.initials: H
authors.lastname: Salzer
authors.forename: T
authors.initials: T
authors.lastname: Kos
authors.forename: O
authors.initials: O
authors.lastname: Wagner
authors.forename: S
authors.initials: S
authors.lastname: Kapiotis
authors.forename: A D
authors.initials: AD
authors.lastname: Kaye
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003276:Contraceptives, Oral / Q000494:pharmacology*; D017373:Cyproterone Acetate / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology*; D045166:Estradiol Congeners / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D006801:Humans; D007231:Infant, Newborn; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology
chemical_list: D003276:Contraceptives, Oral; D045166:Estradiol Congeners; D004997:Ethinyl Estradiol; D017373:Cyproterone Acetate