title: Clinical application of intracytoplasmic sperm injection using in vitro cultured testicular spermatozoa obtained the day before egg retrieval.
abstract: OBJECTIVE
  To study the effect of in vitro culture on the quality of human testicular sperm and the efficiency of intracytoplasmic sperm injection with in vitro cultured testicular sperm.
  
  
  METHODS
  Clinical study.
  
  
  METHODS
  A private IVF center.
  
  
  METHODS
  Twenty consecutively seen IVF patients undergoing testicular biopsies for ICSI.
  
  
  METHODS
  The testicular specimens were cultured in vitro for 24 hours and the isolated spermatozoa were microinjected.
  
  
  METHODS
  Preincubation and postculture sperm motility, and fertilization, implantation, and pregnancy rates after intracytoplasmic sperm injection.
  
  
  RESULTS
  Motility increased from initial nonmotile or twitching sperm to free motile sperm in 18 of 20 cases. The injection of in vitro cultured testicular sperm resulted in a fertilization rate of 58%, an implantation rate of 20%, and a pregnancy rate of 45%.
  
  
  CONCLUSIONS
  A testicular biopsy procedure can be performed the day before egg retrieval. Despite the low initial sperm quality, a high percentage of the prepared testicular sperm showed increased motility after 24 hours of culture. The injection of in vitro cultured testicular sperm into matured oocytes resulted in fertilization, implantation, and pregnancy rates comparable to those obtained with ejaculated sperm.
authors.affiliation: Northwest Center for Infertility and Reproductive Endocrinology, Margate, Florida 33063, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: W S
authors.initials: WS
authors.lastname: Maxson
authors.forename: D I
authors.initials: DI
authors.lastname: Hoffman
authors.forename: S J
authors.initials: SJ
authors.lastname: Ory
authors.forename: M R
authors.initials: MR
authors.lastname: Licht
authors.forename: S
authors.initials: S
authors.lastname: Eager
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D010064:Embryo Implantation; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology; D013737:Testis; D013997:Time Factors; D020858:Tissue and Organ Harvesting