title: Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus.
abstract: The gene encoding factor C (facC), an extracellular signal protein involved in cellular differentiation, was cloned from Streptomyces griseus 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34,523 Da. The mature extracellular 286 amino acid protein (31,038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected facC in several other Streptomyces strains, but database searches failed to identify a protein with significant homology to factor C. Expression of facC from a low-copy-number vector in S. griseus 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.
authors.affiliation: Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Birkó
authors.affiliation: Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sümegi
authors.affiliation: Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vinnai
authors.affiliation: Department of Biochemistry, Leiden University, Gorlaeus Laboratory, PO Box 9502, 2300 RA Leiden, The Netherlands3.
authors.forename: Gilles
authors.initials: G
authors.lastname: van Wezel
authors.affiliation: Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Szeszák
authors.affiliation: Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
authors.forename: Sándor
authors.initials: S
authors.lastname: Vitális
authors.affiliation: Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary2.
authors.forename: Pál T
authors.initials: PT
authors.lastname: Szabó
authors.affiliation: Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary2.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Kele
authors.affiliation: Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary2.
authors.forename: Tamás
authors.initials: T
authors.lastname: Janáky
authors.affiliation: Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
authors.forename: Sándor
authors.initials: S
authors.lastname: Biró
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013305:Streptomyces griseus / Q000737:chemistry / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins; C402566:factor C, Streptomyces