The ABC-exporter genes involved in the lipase secretion are clustered with the genes for lipase, alkaline protease, and serine protease homologues in Pseudomonas fluorescens no. 33.
In Pseudomonas fluorescens no. 33, the lipase gene is clustered with the genes for alkaline protease, AprDEF exporter, and two homologue proteins of Serratia serine proteases (pspA and pspB). Secretion of the lipase and alkaline protease through AprDEF was shown in the Escherichia coli cells. Interestingly, the E. coli cells carrying the pspA gene secreted PspA to the media AprDEF-independently.
Kawai
E
E
Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Idei
A
A
Kumura
H
H
Shimazaki
K
K
Akatsuka
H
H
Omori
K
K
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D010957:Plasmids; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C069816:phage shock protein, Bacteria; D008049:Lipase; C038495:Pseudomonas serine proteinase; D012697:Serine Endopeptidases