title: The Rhizobium etli trpB gene is essential for an effective symbiotic interaction with Phaseolus vulgaris.
abstract: A mutant strain (CTNUX4) of Rhizobium etli carrying Tn5 unable to grow with ammonium as the sole nitrogen source was isolated and characterized. Sequence analysis showed that Tn5 is inserted into a trpB (tryptophan synthase)-homologous gene. When tested on the roots of Phaseolus vulgaris, strain CTNUX4 was able to induce only small, slightly pink, ineffective (Fix-) nodules. However, under free-living conditions, strain CTNUX4 was unable to produce flavonoid-inducible lipo-chitin oligosaccharides (Nod factors) unless tryptophan was added to the growth medium. These data and histological observations indicate that the lack of tryptophan biosynthesis affects the symbiotic behavior of R. etli.
authors.affiliation: International Institute of Genetics and Biophysics, CNR, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Taté
authors.forename: A
authors.initials: A
authors.lastname: Riccio
authors.forename: E
authors.initials: E
authors.lastname: Caputo
authors.forename: M
authors.initials: M
authors.lastname: Cermola
authors.forename: R
authors.initials: R
authors.lastname: Favre
authors.forename: E J
authors.initials: EJ
authors.lastname: Patriarca
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D007887:Fabaceae / Q000235:genetics*; D020043:Genes, Essential; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009876:Operon; D018517:Plant Roots / Q000382:microbiology; D010946:Plants, Medicinal; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics / Q000502:physiology*; D013559:Symbiosis; D014367:Tryptophan Synthase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D014367:Tryptophan Synthase