Preparation and in vitro characterization of HSA-mPEG nanoparticles.
Surface modified human serum albumin (HSA) nanoparticles with a size of approximately 150 nm in diameter were prepared from a PEG-HSA conjugate, methoxy-polyethylene glycol modified human serum albumin (HSA-mPEG) using a coacervation method and crosslinked with glutaraldehyde. The zeta-potential of the surface modified nanoparticles was significantly lower than that of unmodified HSA nanoparticles. The existence of a hydrated steric barrier surrounding the nanoparticles was confirmed by electrolyte and pH induced flocculation tests. The surface modified nanoparticles showed a reduced plasma protein adsorption on the particle surface compared with unmodified particles.
Lin
W
W
Department of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham, UK.
Garnett
M C
MC
Schacht
E
E
Davis
S S
SS
Illum
L
L
D000818:Animals; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D004553:Electric Conductivity; D005427:Flocculation; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D012709:Serum Albumin / Q000737:chemistry*; D012995:Solubility; D013431:Sulfates / Q000494:pharmacology; D013997:Time Factors
D012709:Serum Albumin; D013431:Sulfates; C012036:sodium sulfate; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D005976:Glutaral