title: Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model.
abstract: OBJECTIVE
  To study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women.
  
  
  METHODS
  Controlled study of peritoneal macrophage function.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Patients with a normal peritoneal cavity (n = 15) and with pelvic endometriosis (n = 20) undergoing laparoscopy.
  
  
  METHODS
  Peritoneal macrophages were cocultured with endometrial epithelial and stromal cells; endometrial cell cultures without macrophage coculture acted as controls.
  
  
  METHODS
  Endometrial cellular proliferation measured by 3H-thymidine incorporation.
  
  
  RESULTS
  Endometrial epithelial cells cocultured with peritoneal macrophages from women with endometriosis showed significantly increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.56 versus 1.03 times, respectively, over control) and at 72 hours (1.55 versus 1.10 times over control). Endometrial stromal cells cocultured with peritoneal macrophages from women with endometriosis similarly exhibited increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.65 versus 1.17 times over control) and at 72 hours (1.65 versus 1.21 times over control).
  
  
  CONCLUSIONS
  Peritoneal macrophages of patients with endometriosis stimulate cellular proliferation of endometrial epithelial and stromal cells in vitro.
authors.affiliation: Department of Obstetrics and Gynaecology, National University of Singapore, Singapore. obglohfh@nus.edu.sg
authors.forename: F H
authors.initials: FH
authors.lastname: Loh
authors.forename: A
authors.initials: A
authors.lastname: Bongso
authors.forename: C Y
authors.initials: CY
authors.lastname: Fong
authors.forename: D R
authors.initials: DR
authors.lastname: Koh
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: H Q
authors.initials: HQ
authors.lastname: Zhao
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000473:pathology / Q000503:physiopathology; D002455:Cell Division; D018920:Coculture Techniques; D004715:Endometriosis / Q000473:pathology*; D004717:Endometrium / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D017737:Macrophages, Peritoneal / Q000502:physiology*; D017154:Stromal Cells / Q000473:pathology