Cutting edge: Ig heavy chain 3' HS1-4 directs correct spatial position-independent expression of a linked transgene to B lineage cells.
The Ig H chain locus is regulated by a set of cis-acting elements. Hypersensitive sites (HS) located 3' of the IgH, HS1-4, has been suggested to act as a locus control region (LCR) in cell lines. To assess the proposed role of HS1-4 acting as an LCR, we generated transgenic mice harboring a VH promoter-beta-globin reporter gene linked to the Ig H chain HS1-4 3'regulatory sequences. Transgene expression is strictly confined to B lymphocytes, with no detectable expression outside the B cell lineage in all transgenic founder lines. Furthermore, reporter gene activity is integration independent but not copy number dependent. Thus, additional sequences are required to allow the HS1-4 regulatory region to act as a classical LCR in mice. Our data are discussed in the context of tissue-specific gene expression in B lineage cells.
Chauveau
C
C
Laboratoire d'Immunologie Génétique, Faculté de Médecine, Limoges, France.
Jansson
E A
EA
Müller
S
S
Cogné
M
M
Pettersson
S
S
D020413:3' Untranslated Regions / Q000502:physiology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D018628:Gene Dosage; D005786:Gene Expression Regulation / Q000276:immunology*; D017930:Genes, Reporter; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019901:Locus Control Region / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D019076:Transgenes / Q000276:immunology*
D020413:3' Untranslated Regions; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D005914:Globins