Rescue of influenza A virus from recombinant DNA.
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.
Fodor
E
E
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Devenish
L
L
Engelhardt
O G
OG
Palese
P
P
Brownlee
G G
GG
García-Sastre
A
A
D000818:Animals; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics*; D005821:Genetic Techniques; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism
D004274:DNA, Recombinant; D012367:RNA, Viral; D014764:Viral Proteins