Nucleotide sequence of a marsupial interleukin-10 cDNA from the Australian brushtail possum (Trichosurus vulpecula).
A cDNA encoding a marsupial interleukin-10 (IL-10), was isolated from Australian brushtail possum alveolar macrophages. The cDNA of 1604 bp had an open reading frame of 522 bp coding for a protein of 174 amino acids. Its deduced amino acid sequence had an identity of 60% with cat, 58% with pig, 56% with human and cow, 52% with mouse and 53% with rat IL-10. The expression of IL-10 was up-regulated in both LPS-stimulated and Mycobacterium bovis-infected possum alveolar macrophages.
Wedlock
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DN
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. wedlockn@agresearch.cri.nz
Aldwell
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Buddle
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BM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009893:Opossums / Q000235:genetics*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013552:Swine
D017931:DNA Primers; D018076:DNA, Complementary; D016753:Interleukin-10