Nuclear proliferation in syncytia during avian reovirus replication.
Cultured chick embryonic fibroblasts formed syncytia after infection with avian reovirus (ARV) strain 58-132. Mitotic figures were occasionally observed within the syncytia. In addition, many nuclei in the syncytia incorporated 5-bromo-2'-deoxyuridine (BrdU), a DNA replication marker, indicating that they were in the S-phase of the cell cycle. These observations suggested that the nuclei within ARV-induced syncytia originated from nuclear endomitosis without cell division, as well as from cell fusion.
Tsukamoto
Y
Y
Department of Veterinary Anatomy, College of Agriculture, Osaka Prefecture University, Sakai, Japan. ytsuka@jyui.vet.osakafu-u.ac.jp
Kotani
T
T
Kohama
K
K
Sakuma
S
S
Sasaki
F
F
D000818:Animals; D001717:Birds / Q000821:virology; D002467:Cell Nucleus / Q000000981:diagnostic imaging; D002642:Chick Embryo; D005347:Fibroblasts / Q000000981:diagnostic imaging / Q000821:virology; D015726:Giant Cells / Q000821:virology*; D008938:Mitosis; D018165:Orthoreovirus / Q000502:physiology*; D014463:Ultrasonography; D014779:Virus Replication