Chemical structure of a polysaccharide from Campylobacter jejuni 176.83 (serotype O:41) containing only furanose sugars.
A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained beta-L-arabinose, 6-deoxy-beta-D-altro-heptose (beta-D-6d-altHep) and 6-deoxy-beta-L-altrose (beta-L-6d-Alt), whereas in the minor trisaccharide the beta-L-6d-Alt is replaced by its C-5 epimer alpha-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established: [formula: see text]
Hanniffy
O M
OM
Department of Chemistry, National University of Ireland, Galway.
Shashkov
A S
AS
Moran
A P
AP
Prendergast
M M
MM
Senchenkova
S N
SN
Knirel
Y A
YA
Savage
A V
AV
D016123:Campylobacter jejuni / Q000737:chemistry*; D002648:Child; D003967:Diarrhea / Q000378:metabolism / Q000382:microbiology; D004751:Enteritis / Q000378:metabolism / Q000382:microbiology; D005663:Furans / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D012703:Serotyping
D005663:Furans; D019081:O Antigens; D009844:Oligosaccharides