title: The detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, by gas chromatography/mass spectrometry.
abstract: A sensitive gas chromatography/mass spectrometry method for the detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, is described. The method is based upon solvent extraction followed by derivatisation with methyl-bis-trifluoroacetamide (MBTFA), to give a trifluoroacetyl derivative. A profile of 7-aminoflunitrazepam urinary concentrations following ingestion of 0.5 to 4 mg oral doses is reported. The method has been found to be reproducible and can be used for the confirmation of flunitrazepam administration.
authors.affiliation: Department of Pharmacology and Therapeutics, Trinity College, Dublin.
authors.forename: J
authors.initials: J
authors.lastname: Feely
authors.forename: P V
authors.initials: PV
authors.lastname: Kavanagh
authors.forename: S M
authors.initials: SM
authors.lastname: McNamara
mesh_terms: D002849:Chromatography, Gas; D005445:Flunitrazepam / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry
chemical_list: D005445:Flunitrazepam; C036343:7-aminoflunitrazepam