Monoclonal antibody detects oligodendroglial cell surface protein exhibiting temporal regulation during development.
As tools to study stage-specific surface molecules expressed during the development of oligodendrocytes, we have generated monoclonal antibodies against peanut agglutinin (PNA)-binding glycoproteins isolated by affinity chromatography from the oligodendroglial precursor cell line Oli-neu. In this paper we report the characterization of the monoclonal antibody 7D10. The 7D10 antibody recognizes a 145-kD cell surface glycoprotein expressed by postmitotic multibranched cells of the oligodendroglial lineage. The antigen stains subpopulations of myelin-associated glycoprotein (MAG) and O4-positive cells and is subsequently down-regulated during further differentiation in vitro. The 7D10 antigen is also expressed by a subpopulation of astroglial cells but not by neurons. A truncated form of the protein is released by antigen-expressing cells into the culture supernatant.
Grzenkowski
M
M
Department of Neurobiology, University of Heidelberg, Heidelberg, Germany.
Niehaus
A
A
Trotter
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D009836:Oligodendroglia / Q000276:immunology* / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000494:pharmacology; D011233:Precipitin Tests; D013997:Time Factors
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D019887:Peanut Agglutinin