Differential effect of an Ig mu transgene on development of pre-B cells in fetal and adult SCID mice.
Progression of pro-B lymphocytes to the pre-B stage depends on the expression of a pre-B cell receptor (pre-BCR), consisting of an Ig mu H chain, Ig surrogate light chain, and associated signal transducing chains. Mice that are unable to express a pre-BCR show an arrest of B cell development at the pro-B stage. Such is the case for severe combined immune deficient (SCID) mice in which mu chains are not made because of a defect in V(D)J recombination. When mu chains are made, as in SCID mice bearing a functional mu transgene, then B cell differentiation can proceed to the pre-B stage. However, as reported here, a mu transgene (M54) that promotes development of SCID pre-B cells in adult bone marrow fails to do so in fetal liver. We suggest that a pre-BCR containing the M54 mu chain cannot signal progression of pro-B cells to the pre-B stage in the fetal liver microenvironment.
Bosma
G C
GC
Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA.
Chang
Y
Y
Karasuyama
H
H
Bosma
M J
MJ
D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015536:Down-Regulation; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D005803:Genes, Immunoglobulin; D016654:Genes, RAG-1 / Q000235:genetics; D005838:Genotype; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D008099:Liver / Q000196:embryology / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D008822:Mice, Transgenic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000276:immunology*
D007148:Immunoglobulin mu-Chains