title: Alpha-synuclein immunoreactive Lewy bodies and Lewy neurites in Parkinson's disease are detectable by an advanced silver-staining technique.
abstract: Immunostaining with anti-alpha-synuclein is used to detect Lewy bodies and Lewy neurites in cases of Parkinson's disease and related disorders. To prove that the result of a modern silver method is equivalent to that achieved with immunoreactions for alpha-synuclein, individual sections were successively processed using both methods. The silver-stained sections showed all of the immunoreactive Lewy bodies, and thin Lewy neurites were detected equally well by both techniques. The present study, therefore, points to the capabilities of a modern silver-staining method which is less time consuming and less expensive than immunocytochemical techniques.
authors.affiliation: Department of Anatomy, J. W. Goethe University, Theodor Stern Kai 7, D-60590 Frankfurt/Main, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Sandmann-Keil
authors.forename: H
authors.initials: H
authors.lastname: Braak
authors.forename: M
authors.initials: M
authors.lastname: Okochi
authors.forename: C
authors.initials: C
authors.lastname: Haass
authors.forename: E
authors.initials: E
authors.lastname: Braak
mesh_terms: D006801:Humans; D016631:Lewy Bodies / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010300:Parkinson Disease / Q000473:pathology*; D016622:Silver Staining / Q000379:methods*; D051843:Synucleins; D051844:alpha-Synuclein
chemical_list: D009419:Nerve Tissue Proteins; C497604:SNCA protein, human; D051843:Synucleins; D051844:alpha-Synuclein