Determination of betaine in Lycium chinense fruits by liquid chromatography-electrospray ionization mass spectrometry.
A rapid and sensitive high-performance liquid chromatography-electrospray mass spectrometric method has been developed for the determination of betaine in Lycium chinense fruits. Betaine was analyzed on a system consisting of a NH2 stationary phase and a mobile phase of water-acetonitrile (25:75) by isocratic elution for 40 min. Betaine was identified and quantitated by electrospray ionization mass spectrometry with selected ion monitoring of the protonated ion [Betaine+H]+ and clustered ions [nBetaines+H]+. The limit of detection for betaine by this method was ca. 0.2 ng/ml and the relative standard deviations of the assay (intra- and inter-day) were less than 8.1%.
Shin
Y G
YG
College of Pharmacy, Seoul National University, South Korea.
Cho
K H
KH
Kim
J M
JM
Park
M K
MK
Park
J H
JH
D001622:Betaine / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*
D001622:Betaine