A noninvasive method for quantifying and distinguishing inflammatory skin reactions.
BACKGROUND
The ribonuclease protection assay (RPA) represents a technology that allows detection of small amounts of intact RNA. Recent progress in understanding cytokine networks in the skin suggests that measurements of cytokine mRNA levels could provide a method to distinguish various reactions such as irritant contact dermatitis and allergic contact dermatitis that can occur in the skin.


OBJECTIVE
We attempted to differentiate and quantitate irritant and immunologic skin reactions by measuring mRNA levels.


METHODS
We have used the technique of tape stripping human skin to remove superficial cell layers and have extracted RNA from these skin samples. This RNA was used for RPA analysis.


RESULTS
By means of RPA analysis, we have demonstrated distinct cytokine profiles that appear to discriminate, for example, irritant from immunologic skin reactions.


CONCLUSIONS
We have shown that multiple cytokine mRNA levels can be defined in these RNA samples obtained from the skin. This approach assesses not only the cytokine gene profiles, but at the same time may quantify the severity of common irritant versus allergic skin reactions.
Morhenn
V B
VB
California Skin Research Institute, San Diego 92128, USA.
Chang
E Y
EY
Rheins
L A
LA
D016207:Cytokines / Q000235:genetics; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis* / Q000276:immunology; D017453:Dermatitis, Irritant / Q000175:diagnosis* / Q000276:immunology; D003937:Diagnosis, Differential; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis
D016207:Cytokines; D012333:RNA, Messenger