title: The effect of orally administered viable probiotic and dairy lactobacilli on mouse lymphocyte proliferation.
abstract: Four common Lactobacillus strains were screened for their effects on proliferation of mouse splenic lymphocytes. Mice received perorally 10(9) viable bacteria kg(-1) body weight for 7 days. Lactobacillus acidophilus treatment enhanced ex vivo basal proliferation (by 43%) and B-cell response at suboptimal and optimal concentrations of lipopolysaccharide (LPS) (by 27-28%). Conversely, Lactobacillus casei, Lactobacillus gasseri and Lactobacillus rhamnosus inhibited both basal proliferation (by 14-51%) and mitogen-stimulated lymphoproliferation, particularly at supra-optimal concentrations of concanavalin A (by 43-68%) and LPS (by 23-62%). Therefore, these Lactobacillus strains demonstrate strain-specific effects on B- and T-cells and may also alter the splenocyte sensitivity to the cytotoxic effects of mitogens.
authors.affiliation: Key Centre for Applied and Nutritional Toxicology, RMIT-University, Melbourne, Vic., Australia. pirkka.kirjavainen@utu.fi
authors.forename: P V
authors.initials: PV
authors.lastname: Kirjavainen
authors.forename: H S
authors.initials: HS
authors.lastname: El-Nezami
authors.forename: S J
authors.initials: SJ
authors.lastname: Salminen
authors.forename: J T
authors.initials: JT
authors.lastname: Ahokas
authors.forename: P F
authors.initials: PF
authors.lastname: Wright
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D007778:Lactobacillus; D007779:Lactobacillus acidophilus; D007780:Lacticaseibacillus casei; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology*
chemical_list: D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A