Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum.
The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.
Uparanukraw
P
P
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand. puparanu@sd01.med.cmu.ac.th
Harnnoi
T
T
Dantrakool
A
A
Morakote
N
N
Chaicumpa
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis*; D004524:Eels / Q000469:parasitology; D006031:Glycosylation; D006039:Gnathostoma / Q000276:immunology*; D015801:Helminth Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007814:Larva / Q000276:immunology; D015531:Radioimmunoprecipitation Assay; D017205:Spirurida Infections / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins