Learning induces a CDC2-related protein kinase, KKIAMRE.
To elucidate molecular mechanisms in learning and memory, we analyzed expression of mRNAs in brains of rabbits undergoing eyeblink conditioning. Infusion of the transcription inhibitor actinomycin D into the cerebellar interpositus nucleus reversibly blocked learning but not performance of the conditioned response. Differential display PCR analysis of cerebellar interpositus RNAs from trained and pseudotrained rabbits identified a 207 bp band that was induced with learning. The fragment was used to isolate a cDNA from a lambdagt11 rabbit brain library containing a 1698 bp open reading frame. The deduced amino acid sequence contains the KKIAMRE motif, which is conserved among cell division cycle 2 (cdc2)-related kinases. These results suggest that there is a new category of cdc2-related kinases in the brain whose function may be important in learning and memory.
Gomi
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Neuroscience Program, University of Southern California, Los Angeles, California 90089-2520, USA.
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Finch
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Itohara
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Yoshimi
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Thompson
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D000161:Acoustic Stimulation; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001767:Blinking / Q000187:drug effects; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002529:Cerebellar Nuclei / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D003214:Conditioning, Classical / Q000187:drug effects; D018844:Cyclin-Dependent Kinases; D003609:Dactinomycin / Q000008:administration & dosage / Q000494:pharmacology; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007263:Infusions, Parenteral; D007858:Learning / Q000502:physiology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012016:Reference Values; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*
D012333:RNA, Messenger; D003609:Dactinomycin; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C103570:CDKL2 protein, human; C521371:Cdkl2 protein, mouse; D018844:Cyclin-Dependent Kinases