title: Differential responses of adult cardiac fibroblasts to in vitro biaxial strain patterns.
abstract: Different patterns of extracellular matrix (ECM) remodeling in the heart are thought to be dependent on altered mechanical and chemical conditions and can contribute to cardiac dysfunction. Cardiac fibroblasts are the primary regulators of the ECM and may respond to mechanical factors in vitro. We hypothesized that different types of in vitro strains, e.g. tensile or compressive, can stimulate different functional responses in cultured adult rat cardiac fibroblasts. In this study, we first showed that a single step in strain applied by a uniaxial stretch system stimulated collagen III and fibronectin mRNA levels and transforming growth factor-beta(1) (TGF-beta(1)) activity in the adult phenotype of rat cardiac fibroblasts. Two-dimensional deformations were measured by tracking fluorescent microspheres attached to the substrate and cultured cells. For 10% uniaxial strain, mean principal strains were 0. 104 +/- 0.018 in the direction of stretch and -0.042 +/- 0.013 in the perpendicular direction, verifying that the fibroblasts were simultaneously subjected to tensile (positive) and compressive (negative) strains. Furthermore, these cells were also subjected to area change and to shear. In order to examine the distinct effects of different types of deformation on cardiac fibroblasts, an equibiaxial stretch system was used to apply either pure tensile or compressive area strains, in the absence of shear. Magnitudes of equibiaxial strain were selected to apply local cell area changes identical to those applied in the uniaxial system. Results showed that pure tensile and compressive area strains induced divergent responses in ECM mRNA levels. TGF-beta(1) activity was dependent on the magnitude of applied area strain regardless of the mode of deformation. These findings demonstrate that adult cardiac fibroblasts may respond differently to varied types of mechanical loading, suggesting that ECM remodeling may be locally regulated by specific mechanical stimuli in the heart.
authors.affiliation: Department of Bioengineering, University of California, San Diego, CA 92093, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Delhaas
authors.forename: A D
authors.initials: AD
authors.lastname: McCulloch
authors.forename: F J
authors.initials: FJ
authors.lastname: Villarreal
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005353:Fibronectins / Q000235:genetics; D005786:Gene Expression Regulation; D006321:Heart / Q000502:physiology*; D008297:Male; D009206:Myocardium / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D003094:Collagen