[The antioxidant prevention of disorders in calcium ion metabolism under the action of glutamate on the synaptosomes of the rat cerebral cortex].
An increase of intracellular calcium ion concentration and of the 45Ca2+ entry, a decrease in Na+,K(+)-ATPase activity, and activation of Na+/Ca2+ exchange were shown to be initiated by glutamate in the rat brain cortex synaptosomes. These effects could be prevented with antagonists and blocking agents of the NMDA receptors. Pre-incubation of the synaptosomes with alpha-tocopherol, superoxide dismutase, and ganglioside GM1 was shown to normalise [45Ca2+], the rate of 45Ca2+ entry, and the activity of Na+,K(+)-ATPase in the synaptosomes. The data obtained suggest that calcium ions entering the brain cortex neurones via the NMDA receptors in presence of excessive glutamate, trigger activation of free radical reactions damaging the neurones in ischemia, cerebral lesions, and other pathological conditions.
Avrova
N F
NF
I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Acad. Sci., St. Petersburg, Russia.
Shestak
K I
KI
Zakharova
I O
IO
Sokolova
T V
TV
Tiurina
Iu Iu
IuIu
Tiurin
V A
VA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002128:Calcium Metabolism Disorders / Q000517:prevention & control*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D005732:Gangliosides / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D005732:Gangliosides; D014810:Vitamin E; D018698:Glutamic Acid; D013482:Superoxide Dismutase; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium