title: Dietary heme iron does not prevent postgastrectomy anemia but fructooligosaccharides improve bioavailability of heme iron in rats.
abstract: Gastrectomized rats exhibit iron deficiency anemia. We observed the effects of dietary heme-iron and short chain frucooligosaccharides (Sc-FOS) in relation to prevention of postgastrectomy anemia in rats. Twelve laparotomized (sham-operated) rats were fed iron-citrate (control) as iron source diet without or with Sc-FOS (75 g/kg of diet) and twenty four totally gastrectomized (Bilroth II) rats, were fed a iron-citrate (control) or heme-iron (heme) as iron source diet without or with Sc-FOS (75 g/kg of diet) for 4 weeks. All rats received an intramuscular injection of vitamin B-12 every two weeks. Tail blood was collected every other week for determination of hematocrit and hemoglobin concentration. At the end of the experiment, the rats were killed and whole blood was collected. The total gastrectomy induced the postgastrectomy anemia. Dietary Sc-FOS increase iron absorption and thereby prevented completely this anemia in gastrectomized rats fed the control diet but this effect of Sc-FOS in rats fed heme diet was not complete. Dietary heme iron could not prevent postgastrectomy anemia itself, but fructooligosaccharides improve bioavailability of not only non-heme iron such as iron-citrate, but also heme-iron in rats.
authors.affiliation: Nutritional Science Center, Meiji Seka Kaisha, Ltd., Saitama, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Takasaki
authors.forename: M
authors.initials: M
authors.lastname: Uehara
authors.forename: T
authors.initials: T
authors.lastname: Tokunaga
authors.forename: T
authors.initials: T
authors.lastname: Adachi
mesh_terms: D000740:Anemia / Q000517:prevention & control*; D000818:Animals; D001682:Biological Availability; D001835:Body Weight / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005632:Fructose; D005743:Gastrectomy / Q000009:adverse effects*; D006400:Hematocrit; D006418:Heme / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis; D007501:Iron / Q000493:pharmacokinetics*; D019266:Iron, Dietary / Q000627:therapeutic use*; D008099:Liver / Q000737:chemistry; D008297:Male; D009844:Oligosaccharides / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D019266:Iron, Dietary; D009844:Oligosaccharides; D005632:Fructose; D006418:Heme; D007501:Iron