Structural analysis of 13 neutral oligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Rana temporaria.
Amphibian eggs are always surrounded by an extracellular matrix, named the jelly coat. This is mainly composed of a highly O-glycosylated, mucin-type glycoprotein. This work has consisted of isolating O-linked neutral oligosaccharides from oviducal mucin of Rana temporaria, with a view to determining their primary structure. Hence, these carbohydrate chains have been released by alkaline borohydride treatment leading to stable glycans. The oligosaccharide-alditols have been purified by ion-exchange chromatography and separated by HPLC. The primary structure of 13 of these carbohydrate chains have been obtained by 1D/2D 1H-NMR spectroscopy and methylation analyses, in combination with MALDI-TOF mass spectroscopy. The results confirm what has been observed for six other amphibians about the species-specificity of the carbohydrate moieties and their likely involvement in the species-specific gamete recognition.
Coppin
A
A
Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de Recherche Scientifique no. 8576, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Maes
E
E
Morelle
W
W
Strecker
G
G
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009865:Oocytes / Q000737:chemistry*; D010057:Oviducts / Q000378:metabolism*; D011896:Rana temporaria / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013402:Sugar Alcohols / Q000737:chemistry* / Q000302:isolation & purification
D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols