Extinction coefficients and midpoint potentials of cytochrome c(6) from the cyanobacteria Arthrospira maxima, Microcystis aeruginosa, and Synechocystis 6803.
Cytochrome c(6) is a soluble heme protein that serves as a photosynthetic electron transport component in cyanobacteria and algae, carrying electrons from the cytochrome bf complex to photosystem I. The rapid accumulation of cytochrome c(6) sequence data from a wide range of species, combined with significant advances in determining high resolution three-dimensional structures, provides a powerful database for investigating the relationship between structure and function. The fact that the gene encoding cytochrome c(6) can be readily modified in a number of species adds to the usefulness of cytochrome c(6) as a tool for comparative analysis. Efforts to relate cytochrome c(6) sequence information to structure, and structural information to function depend on knowledge of the physical and thermodynamic properties of the cytochrome from different species. To this end we have determined the optical extinction coefficient, the oxidation/reduction midpoint potential, and the pH dependence of the midpoint potential of cytochrome c(6) isolated from three cyanobacteria, Arthrospira maxima, Microcystis aeruginosa, and Synechocystis 6803.
Cho
Y S
YS
Department of Plant Biology, University of Illinois, Urbana, IL 61801, USA.
Wang
Q J
QJ
Krogmann
D
D
Whitmarsh
J
J
D000458:Cyanobacteria / Q000201:enzymology*; D003580:Cytochromes / Q000737:chemistry* / Q000302:isolation & purification; D045348:Cytochromes f; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D046931:Microcystis; D010084:Oxidation-Reduction; D011199:Potentiometry; D013053:Spectrophotometry
D003580:Cytochromes; D045348:Cytochromes f