Natural genetic transformation in the rumen bacterium Streptococcus bovis JB1.
Natural transformation of Streptococcus bovis JB1 was demonstrated after development of competence in normal culture medium. Transformation efficiencies were not significantly increased when heat-inactivated horse serum was added to the medium before growth. This is the first time that a resident rumen bacterial species has been shown to be naturally transformable. Transformation allowed the acquisition of plasmids or integration of sequences into the chromosome. No transformation was observed in the presence of undiluted autoclaved or filter-sterilised ovine rumen fluid or filter-sterilised ovine saliva, suggesting that transformation in the ruminant digestive tract is a rare event, although transformation was observed in the presence of 1% and 0.5% filter-sterilised rumen fluid. The use of natural transformation of S. bovis should facilitate further molecular biological studies on this species.
Mercer
D K
DK
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, UK. d.mercer@rri.sari.ac.uk
Melville
C M
CM
Scott
K P
KP
Flint
H J
HJ
D000818:Animals; D012417:Rumen / Q000382:microbiology*; D012463:Saliva / Q000502:physiology; D012756:Sheep; D016985:Streptococcus bovis / Q000235:genetics*; D014169:Transformation, Bacterial