title: Immunolocalization of Bone Morphogenetic Protein-2 and -3 and Osteogenic Protein-1 during murine tooth root morphogenesis and in other craniofacial structures.
abstract: The distribution of Bone Morphogenetic Protein-2, and -3 (BMP-2 and BMP-3) and Osteogenic Protein-1 (OP-1, also known as BMP-7) during root morphogenesis and in other craniofacial structures was examined in sections of 12- to 18-d-old mouse heads using polyclonal and monoclonal antibodies. BMP-3 and OP-1 were localized in alveolar bone, cementum, and periodontal ligament, whereas BMP-2 was only localized in the alveolar bone of periodontium. All three BMPs were localized in predentine, dentine, odontoblasts, osteoblasts, osteocytes, osteoid, cartilage, chondrocytes and spiral limbus. BMP-2 and OP-1 were also localized in spiral ligament and interdentate cells of the cochlea, whilst BMP-3 was restricted to the spiral ganglion. BMP-3 was also localized in ducts of submandibular and sublingual salivary glands, acini of the lacrimal gland, Purkinje cells in the cerebellum, nerve fibres of the cerebellum and brain, afferent cells of the dorsal root ganglia, inferior alveolar nerve, and peripheral processes of the vestibulocochlear nerve. OP-1 was also localized in hair and whisker follicles, sclera of the eye and in ameloblasts. The demonstration of BMP-3 in the nervous system suggests that this protein may be neurotrophic during development and maintenance of the nervous system. The composite expression of BMPs/OPs during periodontal tissue morphogenesis suggests that optimal therapeutic regeneration may entail the combined use of different BMPs/OPs.
authors.affiliation: Bone Research Laboratory, Medical Research Council/University of the Witwatersrand, Medical School, Johannesburg, South Africa.
authors.forename: G
authors.initials: G
authors.lastname: Thomadakis
authors.forename: L N
authors.initials: LN
authors.lastname: Ramoshebi
authors.forename: J
authors.initials: J
authors.lastname: Crooks
authors.forename: D C
authors.initials: DC
authors.lastname: Rueger
authors.forename: U
authors.initials: U
authors.lastname: Ripamonti
mesh_terms: D000344:Afferent Pathways / Q000033:anatomy & histology; D000539:Alveolar Process / Q000033:anatomy & histology; D000818:Animals; D001857:Bone Matrix / Q000033:anatomy & histology; D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000032:analysis*; D002356:Cartilage / Q000033:anatomy & histology; D019902:Chondrocytes / Q000166:cytology; D003053:Cochlear Duct / Q000033:anatomy & histology; D003739:Dental Cementum / Q000033:anatomy & histology; D003804:Dentin / Q000033:anatomy & histology; D006133:Growth Substances / Q000032:analysis*; D008340:Mandibular Nerve / Q000033:anatomy & histology; D051379:Mice; D008815:Mice, Inbred Strains; D009412:Nerve Fibers / Q000648:ultrastructure; D009804:Odontoblasts / Q000166:cytology; D009805:Odontogenesis / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology; D010011:Osteocytes / Q000166:cytology; D010513:Periodontal Ligament / Q000033:anatomy & histology; D012469:Salivary Glands / Q000033:anatomy & histology; D013136:Spiral Ganglion / Q000033:anatomy & histology; D014092:Tooth Root / Q000033:anatomy & histology* / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000032:analysis*; D014722:Vestibule, Labyrinth / Q000033:anatomy & histology
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D016212:Transforming Growth Factor beta