Characterization of microtubule-associated proteins in teleosts.
Although microtubules are known to play an important role in many cellular processes, they have been virtually neglected in fish. In this report, microtubule-associated proteins (MAPs) in fish (teleost) were characterized using antibodies (Abs) directed against the mammalian MAPs tau, MAP1A and B, and MAP 2. Two different populations of tau-like proteins (TLPs) were found in fish brain using the anti-tau Abs Tau-1, Tau-2, tau5', and tau3'. The TLPs that were recognized by Tau-1, Tau-2, and tau5' were (1) heat-stable; (2) the same molecular weight as mammalian TLPs: 59-62 kDa; (3) not enriched in microtubules prepared from catfish brain; and (4) localized to the cell body of neurons in fish brains. While the TLPs recognized by tau3' Abs were (1) heat-stable; (2) lower molecular weight than mammalian TLPs: 32-55 vs. 50-65 kDa; (3) enriched in microtubule fractions prepared from catfish brain, and (4) localized to the axons of neurons. These results are consistent with two different populations of TLPs being present in fish brains. While MAP2 was found to be approximately the same molecular weight, 250 kDa, in zebrafish and goldfish as in mammals and to be distributed to dendrites in the fish brain, both MAP1A and MAP1B were found to be about 25% the mass of their mammalian homologs. These results suggest that MAPS in fish have some characteristics similar to their mammalian counterparts, but also possess some unique properties that require further study to elucidate their function.
Tomasiewicz
H G
HG
Department of Cell Biology, Emory University, Atlanta 30322, USA.
Wood
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000473:pathology; D002397:Catfishes / Q000378:metabolism; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D005260:Female; D005399:Fishes; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D008870:Microtubules / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D015027:Zebrafish; D016875:tau Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; C490546:Map1a protein, rat; D008869:Microtubule-Associated Proteins; C085284:microtubule-associated protein 1B; D016875:tau Proteins