Comparison of the Quantiplex version 3.0 assay and a sensitized Amplicor monitor assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma samples.
This study evaluated correlation and agreement between version 3 of the Quantiplex human immunodeficiency virus type 1 (HIV-1) RNA assay (v3 branched DNA [bDNA]) and a sensitized Amplicor HIV-1 Monitor assay (reverse transcription [RT]-PCR) for the measurement of HIV RNA. Three hundred eighteen samples from 59 randomly selected, HIV-1-seropositive persons on various drug protocols from the National Institute of Allergy and Infectious Diseases HIV outpatient clinic were studied. The results indicate that v3 bDNA and RT-PCR are highly correlated (r = 0.98) and are in good agreement (mean difference in log(10) copies/ml +/- 2 standard deviations = 0.072 +/- 0.371). The relationship between values obtained by both assays is given by the following equation: log(10)v3 bDNA = -0.0915 + 1. 0052. log(10)RT-PCR. This represents a 1.026-fold difference between log(10)RT-PCR values and log(10)v3 bDNA values.
Highbarger
H C
HC
Virus Isolation Laboratory, Clinical Services Program, Science Applications International Corporation-Frederick, Frederick, Maryland 21702, USA.
Alvord
W G
WG
Jiang
M K
MK
Shah
A S
AS
Metcalf
J A
JA
Lane
H C
HC
Dewar
R L
RL
D019380:Anti-HIV Agents / Q000627:therapeutic use; D005069:Evaluation Studies as Topic; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D014766:Viremia / Q000188:drug therapy / Q000821:virology; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
D019380:Anti-HIV Agents; D012367:RNA, Viral