title: Proliferation and differentiation of rat dorsal prostatic epithelial cells in collagen gel matrix culture, focusing upon effects of adipocytes.
abstract: BACKGROUND
  Prostatic epithelial cells organize functional acinus structures under epithelial extracellular matrix and epithelial-stromal cell interactions. Recently, the adipose tissue, which surrounds and even exists within the prostate, has been suggested to affect the differentiation and proliferation of some cell types. Therefore, tissue fragments, which consist mainly of epithelial and fibromuscular stromal cells, were cultured in three-dimensional collagen gel matrix culture with adipocytes.
  
  
  METHODS
  Tissue fragments of rat dorsal prostate, including both epithelial and fibromuscular stromal components, were cultured in collagen gel with or without adipocytes. Epithelial cell differentiation was evaluated with the reconstruction of acinus-like structures and with immunohistochemistry of rat dorsal prostate-specific proteins, dorsal protein-1 and probasin. The proliferation was examined by uridine uptake.
  
  
  RESULTS
  Under coculture of the fragments and adipocytes, epithelial cells reconstructed more differentiated acinus-like structures surrounded by fibromuscular stromal cells than tissue fragment culture without adipocytes. Dorsal protein-1 and probasin expressions of epithelial cells in this coculture system were the same as in rat prostate in vivo. In the coculture, epithelial cells had a higher proliferation activity.
  
  
  CONCLUSIONS
  These results indicate that adipocytes promote proliferation and differentiation of prostatic epithelial cells. Our new culture model with adipocytes suggests the importance of cell-cell interactions, including those of epithelial cells and adipocytes.
authors.affiliation: Department of Urology, Saga Medical School, Nabeshima, Saga City, Japan. g9512@smsnet.saga-med.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Tokuda
authors.forename: S
authors.initials: S
authors.lastname: Toda
authors.forename: Z
authors.initials: Z
authors.lastname: Masaki
authors.forename: H
authors.initials: H
authors.lastname: Sugihara
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D003094:Collagen; D016207:Cytokines; D004847:Epithelial Cells / Q000166:cytology* / Q000000981:diagnostic imaging; D008297:Male; D008854:Microscopy, Electron; D011467:Prostate / Q000166:cytology* / Q000000981:diagnostic imaging; D051381:Rats; D017208:Rats, Wistar; D014463:Ultrasonography
chemical_list: D016207:Cytokines; D003094:Collagen