title: Role of multiple efflux pumps in Escherichia coli in indole expulsion.
abstract: Escherichia coli chromosome encodes several multidrug transporters. Despite their protective function against antibacterial agents, the specific physiological actions of these transporters are not fully understood. E. coli produces indole, a metabolite of tryptophan, under physiological conditions. Defined inactivation of the acrEF gene, the product of which is known as an energy-dependent multiple drug efflux pump, decreased indole excretion while reintroduction of the acrEF gene restored it. A DeltaacrEF mutant accumulated more intracellular indole than the parent. This mutant was more susceptible to the growth-inhibitory effect of indole than the parent. These results indicate that the AcrEF system plays a significant role in indole efflux.
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawamura-Sato
authors.forename: K
authors.initials: K
authors.lastname: Shibayama
authors.forename: T
authors.initials: T
authors.lastname: Horii
authors.forename: Y
authors.initials: Y
authors.lastname: Iimuma
authors.forename: Y
authors.initials: Y
authors.lastname: Arakawa
authors.forename: M
authors.initials: M
authors.lastname: Ohta
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D008826:Microbial Sensitivity Tests
chemical_list: D002352:Carrier Proteins; D007211:Indoles