title: Co-transfected SV40 origin of replication activates expression from SV40 promoterless constructs.
abstract: Co-transfection with expression plasmids is widely used to control DNA uptake efficiency in transient transfection experiments. However, a number of problems have been associated with their use. Here, we describe the activation of expression of constructs not containing the simian virus 40 (SV40) origin of replication (ori) by co-transfection in COS-7 cells with plasmids containing the SV40 ori. This effect has consequences for the use of such plasmids to control transfection efficiency.
authors.affiliation: University of Nottingham, Loughborough, Leics, England, UK.
authors.forename: A
authors.initials: A
authors.lastname: Flint
authors.forename: S
authors.initials: S
authors.lastname: Kaluz
authors.forename: M
authors.initials: M
authors.lastname: Kaluzova
authors.forename: L
authors.initials: L
authors.lastname: Sheldrick
authors.forename: P
authors.initials: P
authors.lastname: Fisher
authors.forename: K
authors.initials: K
authors.lastname: Derecka
mesh_terms: D000818:Animals; D019556:COS Cells; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011960:Receptors, Estrogen / Q000235:genetics; D018741:Replication Origin / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D011960:Receptors, Estrogen; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase; D001616:beta-Galactosidase