Structural studies utilizing 13C-enrichment of the O-antigen polysaccharide from the enterotoxigenic Escherichia coli O159 cross-reacting with Shigella dysenteriae type 4.
The structure of the O-antigen polysaccharide from Escherichia coli O159 has been determined using primarily NMR spectroscopy of the 13C-enriched polysaccharide. The sequence of the sugar residues could be determined by heteronuclear multiple bond connectivity NMR experiments. The polysaccharide is composed of a pentasaccharide repeating unit with the following structure: [sequence: see text] Matrix assisted laser desorption ionization mass spectrometry was performed on intact lipopolysaccharide and from the resulting molecular mass the O-antigen part was estimated to contain approximately 23 repeating units. Cross-reactivity of this O-antigen to that of Shigella dysenteriae type 4 was confirmed using enzyme-linked immunoabsorbant assay.
Linnerborg
M
M
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Weintraub
A
A
Widmalm
G
G
D000328:Adult; D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D002449:Cell Aggregation; D002648:Child; D003429:Cross Reactions; D003967:Diarrhea / Q000382:microbiology*; D003968:Diarrhea, Infantile / Q000382:microbiology; D004196:Disease Outbreaks; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012762:Shigella dysenteriae / Q000145:classification / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014774:Virulence
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial