Expression of E-selectin, P-selectin, and intercellular adhesion molecule-1 during experimental murine listeriosis.
The expression of adhesion molecules E-selectin, P-selectin, and intercellular adhesion molecule-1 (ICAM-1) was immunohistochemically investigated during the course of experimental murine listeriosis. Infection was monitored by microbiological count of blood, liver, and spleen. After an early generalized expression of P-selectin and ICAM-1, a later regulation occurred specifically to areas of inflammation. Expression of E-selectin was faint and inconstantly detected in all of the studied organs. In the liver, typical lesions of murine listeriosis were related to the expression of ICAM-1 on sinusoidal endothelial cells and the biliary system and to the de novo expression of P-selectin in hepatic portal vessels. Inflammation in the spleen was related to the expression of ICAM-1 on red pulp sinusoidal cells, especially in the marginal sinus. High endothelial venules of inflamed lymph nodes also expressed P-selectin and ICAM-1. Lesions in the central nervous system appeared on day 3 after infection as a pyogranulomatous leptomeningitis associated with an intense expression of P-selectin and ICAM-1 in meningeal vessels, especially those in the hippocampal sulcus, suggesting a way through which inflammation initially reach the central nervous system during experimental murine listeriosis. Leptomeningitis was followed by the presence of ventriculitis, which was related to the up-regulation of ICAM-1 on choroid plexus epithelial cells, periventricular vessels and ependymal cells. Up-regulation of P-selectin and ICAM-1 during experimental murine listeriosis could play an important role in the recruitment of leukocytes, especially to the liver, lymphoid organs, and central nervous system.
López
S
S
Department of Pathology, Veterinary School, Bellaterra, Barcelona, Spain. santiago.lopez@cc.uab.es
Prats
N
N
Marco
A J
AJ
D000818:Animals; D016470:Bacteremia / Q000150:complications; D002490:Central Nervous System / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D019040:E-Selectin / Q000096:biosynthesis*; D005260:Female; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008584:Meningitis, Listeria / Q000150:complications / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D019007:P-Selectin / Q000096:biosynthesis*; D013154:Spleen / Q000382:microbiology / Q000473:pathology
D019040:E-Selectin; D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1