title: Carrier properties of a protein derived from outer membrane protein A of Klebsiella pneumoniae.
abstract: We have recently cloned a new protein, recombinant P40 (rP40). When tested in vivo after conjugation to a B-cell epitope, rP40 induces an important antibody response without the need for adjuvant. To characterize its potency, this carrier protein was coupled to a peptide derived from respiratory syncytial virus attachment G protein (G1'). After immunization of mice with the rP40-G1' conjugate, strong antipeptide antibodies were detected, whereas peptide alone was not immunogenic. To emphasize the carrier properties of rP40, a polysaccharide derived from Haemophilus influenzae type b (Hib) was coupled to it. Immunoglobulin G responses against the Hib polysaccharide were observed after coupling to rP40. Interestingly, an antipeptide antibody response was observed despite preexisting anti-rP40 antibodies generated by preimmunization with rP40. In addition, rP40 compares well with the reference carrier protein, tetanus toxoid (TT), since antibody responses of equal intensity were observed when a peptide or a polysaccharide was coupled to TT and rP40. Moreover, rP40 had advantages compared to TT; e.g., it induced a mixed Th1/Th2 response, whereas TT induced only a Th2 profile. Together, the results indicate that rP40 is a novel carrier protein with potential for use as an alternative carrier for human vaccination.
authors.affiliation: Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France.
authors.forename: I
authors.initials: I
authors.lastname: Rauly
authors.forename: L
authors.initials: L
authors.lastname: Goetsch
authors.forename: J F
authors.initials: JF
authors.lastname: Haeuw
authors.forename: C
authors.initials: C
authors.lastname: Tardieux
authors.forename: T
authors.initials: T
authors.lastname: Baussant
authors.forename: J Y
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: N
authors.initials: N
authors.lastname: Corvaia
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D016667:Bacterial Capsules; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D005260:Female; D015707:HN Protein; D018073:Haemophilus Vaccines / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013744:Tetanus Toxin / Q000276:immunology; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D015707:HN Protein; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins; D013744:Tetanus Toxin; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G; C078167:OMPA outer membrane proteins