Application of the high-performance liquid chromatographic method for separation, purification and characterisation of p-bromophenylacetylurea and its metabolites.
This study presents a HPLC method for the separation and purification of p-bromophenylacetylurea (BPAU) and its metabolites. The method effectively separated and purified BPAU and its metabolites. Three metabolites of BPAU, M1, M2 and M3 were characterised by mass spectroscopy and nuclear magnetic resonance. They are named as N'-hydroxy-p-bromophenylacetylurea, 4-(4-bromophenyl)-3-oxapyrrolidine-2,5-dione and N'-methyl-p-bromophenylacetylurea, respectively. The major metabolic pathways of BPAU were proposed. The establishment of the HPLC method and characterisation of BPAU metabolites make it possible for further pharmacokinetic studies to explore the mechanism of BPAU-induced delayed neuropathy.
Xu
J
J
Department of Biological and Biomedical Sciences, Faculty of Applied Sciences, University of the West of England, Bristol, UK. Jinsheng.Xu@uwe.ac.uk
Lamb
J H
JH
Jukes
R
R
Purcell
W M
WM
Ray
D E
DE
D000818:Animals; D000927:Anticonvulsants / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D014508:Urea / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism
D000927:Anticonvulsants; C004506:4-bromophenylacetylurea; D014508:Urea