NK binding capacity and lytic activity depend on the expression of ICAM-1 on target bone tumours.
Osteosarcoma cell lines are differently lysed by natural killer (NK) lymphocytes. A critical step in the lytic process is the recognition and attachment of effector to target cells. To determine binding capacity and lytic activity of NK cells, we investigated the distribution and role of ICAM-1, 2 and 3 on two osteosarcoma cell lines (HOS and Saos-2) in basal conditions and after TNFalpha treatment. Modulation of ICAM-1 after TNFalpha treatment modified the binding capacity of NK cells to osteosarcoma target cells. This modulation process appears to play a critical role in determining the susceptibility of these cells to NK-mediated lysis.
Meneghetti
A
A
Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti-IOR, 40136 Bologna, Italy.
Mariani
E
E
Santi
S
S
Riccio
M
M
Cattini
L
L
Paoletti
S
S
Facchini
A
A
D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000943:Antigens, Differentiation; D001859:Bone Neoplasms / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000187:drug effects / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D012516:Osteosarcoma / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; C065289:ICAM2 protein, human; C483616:ICAM3 protein, human; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1