Characterization of an alpha-tubulin gene of Cryptosporidium parvum.
A gene encoding an alpha-tubulin of Cryptosporidium parvum was isolated and characterized. It had no introns, and encoded a 441-amino acid protein whose predicted ORF represented a typical alpha-tubulin protein with a MW of 50.5 kDa. This tubulin had an amino acid sequence similarity with Apicomplexa Plasmodium falciparum and Toxoplasma gondii higher than 88% and shared a number of conserved motifs.
Bonafonte
M T
MT
VA Medical Center, Decatur, Georgia 30033, USA.
Garmon
D
D
Mead
J R
JR
D000818:Animals; D015153:Blotting, Western; D016785:Cryptosporidium parvum / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016053:RNA, Protozoan / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism*
D016054:DNA, Protozoan; D016053:RNA, Protozoan; D014404:Tubulin