Probability description of ligand-receptor interactions. Evaluation of reliability of events with small and supersmall doses. I. Kinetics of ligand-receptor interactions.
We have developed mathematical methods for describing ligand-receptor interactions (LRI) using Markov chains. Under some conditions, the mean value of ligand-receptor complexes obtained using Markov chains coincides with that obtained from the law of mass action. Using the calculated ratio of standard deviation to mean number of ligand-receptor complexes, we show that with small concentrations of ligand-receptor complexes LRI must be described using probability methods. Using data from the literature, we show that LRI description using the mass-action law under these conditions can cause significant errors in interpretation of experimental data.
Gurevich
K G
KG
Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. GKG@bio.chem.msu.ru
Varfolomeev
S D
SD
D001665:Binding Sites; D001792:Blood Platelets / Q000378:metabolism; D004088:Dihydroergotoxine / Q000097:blood; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008390:Markov Chains; D008962:Models, Theoretical; D011336:Probability; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D008024:Ligands; D011956:Receptors, Cell Surface; D004088:Dihydroergotoxine