Potassium channel-mediated vasorelaxation is impaired in experimental renal failure.
Chronic renal failure is associated with increased cardiovascular morbidity and abnormal arterial tone, but the underlying pathophysiological mechanisms are poorly understood. Therefore, we studied the responses of isolated mesenteric arterial rings from Wistar-Kyoto rats in standard organ chambers 6 wk after subtotal (5/6) nephrectomy or sham operation. Subtotal nephrectomy resulted in a 1.7-fold elevation of plasma urea nitrogen, whereas blood pressure was not significantly affected. Endothelium-mediated relaxations of norepinephrine-precontracted rings to ACh were impaired in renal failure rats. The nitric oxide (NO) synthase inhibitor N(G)-nitro-L-arginine methyl ester inhibited relaxations to ACh more effectively in the renal failure group, whereas the cyclooxygenase inhibitor diclofenac did not significantly affect the response in either group. Inhibition of Ca(2+)-activated K(+) channels by charybdotoxin and apamin attenuated NO synthase- and cyclooxygenase-resistant relaxations to ACh in control but not renal failure rats and abolished the difference between these groups. Endothelium-independent relaxations to isoproterenol and cromakalim, vasodilators acting via beta-adrenoceptors and ATP-sensitive K(+) channels, respectively, were impaired in the renal failure group, whereas relaxations to the NO donor nitroprusside were similar in both groups. In conclusion, endothelium-mediated relaxation in renal failure rats was impaired in the absence and presence of NO synthase and cyclooxygenase inhibition but not with prevented smooth muscle hyperpolarization. Endothelium-independent relaxations to isoproterenol and cromakalim were also attenuated after 5/6 nephrectomy. These results suggest that impaired vasodilatation in experimental renal failure could be attributed to reduced relaxation via arterial K(+) channels.
Kalliovalkama
J
J
Department of Pharmacological Sciences, University of Tampere, Tampere University Hospital, FIN-33101 Tampere, Finland. jarkko.kalliovalkama@uta.fi
Jolma
P
P
Tolvanen
J P
JP
Kähönen
M
M
Hutri-Kähönen
N
N
Saha
H
H
Tuorila
S
S
Moilanen
E
E
Pörsti
I
I
D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D004326:Drinking; D007676:Kidney Failure, Chronic / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D008297:Male; D008638:Mesenteric Arteries / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D009929:Organ Size; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D011921:Rats, Inbred WKY; D014664:Vasodilation
D015221:Potassium Channels