title: A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay.
abstract: A cytogenetic study was performed in 215 nuclear power plant workers occupationally exposed to radiation using the micronucleus-centromere assay for peripheral blood lymphocytes. As control population served administrative staff with yearly doses below 1 mSv. The increase of the micronucleus frequency with age, observed in the non-smoking control population, is mainly due to an enhanced number of centromere-positive micronuclei, pointing to an increased chromosome loss. No differences in the number of micronuclei, centromere-positive and centromere-negative micronuclei between smokers and non-smokers are observed. An analysis of the micronucleus data vs. the dose accumulated over the 10 years preceding the venepuncture shows no significant clastogenic or aneuploidogenic effects of the exposure in the studied population which is representative for workers in the nuclear industry at present. According to the linear fits to our data an increase of the micronucleus frequency pro rata 0.5 per 1000 binucleated cells per year, related to the centromere-negative micronuclei, may be expected for workers with the maximal tolerable dose of 20 mSv/year.
authors.affiliation: Department of Biomedical Physics and Radiation Protection, University of Ghent, Proeftuinstraat 86, B-9000, Ghent, Belgium. hubert.thierens@rug.ac.be
authors.forename: H
authors.initials: H
authors.lastname: Thierens
authors.forename: A
authors.initials: A
authors.lastname: Vral
authors.forename: M
authors.initials: M
authors.lastname: Barbé
authors.forename: B
authors.initials: B
authors.lastname: Aousalah
authors.forename: L
authors.initials: L
authors.lastname: De Ridder
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D002503:Centromere / Q000528:radiation effects*; D020732:Cytogenetic Analysis; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D011829:Radiation Dosage