The adjuvant effect of a single dose of interleukin-12 on murine immune responses to live or killed Brucella abortus strain RB51.
This study was designed to determine if a single 0.5 microg administration of recombinant murine interleukin-12 (IL-12) would influence immune responses of mice vaccinated with live or killed Brucella abortus strain RB51 (SRB51). Mice were vaccinated intraperitoneally with 5 x 10(8) cfu of live or gamma-irradiated SRB51 bacteria alone, or in combination with 0.5 microg of IL-12. Control mice received saline or 0.5 microg of IL-12. Serologic responses and spleen weights after vaccination were greater in mice vaccinated with live SRB51 when compared to mice receiving killed SRB51 or control treatments. Administration of a single dose of IL-12 as a vaccine adjuvant did not influence immune responses, clearance of live SRB51, or resistance against B. abortus strain 2308 (S2308) challenge. The results of this study suggest that a single administration of 0.5 microg of IL-12 at the time of vaccination does not have significant adjuvant effects on vaccine-induced immune responses against live or killed Brucella.
Lee
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IK
National Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA.
Olsen
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SC
Kehrli
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Bolin
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CA
D000276:Adjuvants, Immunologic; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002003:Brucella abortus / Q000472:pathogenicity*; D002007:Brucellosis, Bovine / Q000517:prevention & control*; D002417:Cattle; D004334:Drug Administration Schedule; D018664:Interleukin-12 / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D015164:Vaccines, Inactivated
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D015164:Vaccines, Inactivated; D018664:Interleukin-12