Involvement of interleukin-10 (IL-10) and viral IL-6 in the spontaneous growth of Kaposi's sarcoma herpesvirus-associated infected primary effusion lymphoma cells.
Primary effusion lymphoma (PEL) is a distinct type of lymphoma associated with Kaposi's sarcoma-associated herpesvirus (KSHV) infection. To determine the factors responsible for the unrestrained proliferation of PEL, we have studied the growth factor requirements of the PEL-derived BCBL-1 and BC-1 cell lines. Both cell lines were found to be autocrine growth factor dependent and to release human interleukin-6 (IL-6), viral IL-6 (vIL-6), and human IL-10 in the culture supernatant. To establish whether these cytokines contribute to autocrine growth, neutralizing antibodies against human IL-6, vIL-6, human IL-10, and soluble IL-10 receptor were used. These experiments showed that human IL-10 and, to a lesser degree, vIL-6 serve as autocrine growth factors for BCBL-1 and BC-1 cells. Thus, human IL-10 and vIL-6 are growth factors released and used by PEL cells for autonomous proliferation and may be critical to the development and progression of PEL.
Jones
K D
KD
Division of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
Aoki
Y
Y
Chang
Y
Y
Moore
P S
PS
Yarchoan
R
R
Tosato
G
G
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D019898:Autocrine Communication / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division; D017077:Culture Media, Conditioned / Q000494:pharmacology; D019288:Herpesvirus 8, Human / Q000502:physiology*; D006801:Humans; D007106:Immune Sera; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D016393:Lymphoma, B-Cell / Q000473:pathology* / Q000821:virology; D018123:Receptors, Interleukin / Q000037:antagonists & inhibitors / Q000276:immunology; D053701:Receptors, Interleukin-10; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D000093242:Interleukin-6 Inhibitors
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D007106:Immune Sera; D016753:Interleukin-10; D015850:Interleukin-6; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D014764:Viral Proteins; D000093242:Interleukin-6 Inhibitors