Membrane integrity, mitochondrial activity, ATP content, and motility of the European catfish (Silurus glanis) testicular spermatozoa after freezing with different cryoprotectants.
The extent of cellular damage was investigated after freeze-thawing of the European catfish testicular sperm with various cryoprotectants. The best protection was given by dimethylacetamide (10 and 15%) in a sucrose solution. Under these conditions, the percentage of cells with an intact membrane was high (90%), and the protection of the activity of the mitochondria was medium (47%). It was shown that the addition of dimethylacetamide largely increased the ATP content of the spermatozoa. It is suggested that this phenomenon is a decisive factor for the freezing resistance of European catfish testicular spermatozoa in the presence of dimethylacetamide (60% motility after thawing versus 90% before freezing).
Ogier de Baulny
B
B
Biodiversité et Environnement, Campus de Beaulieu, Rennes Cedex, 35042, France.
Labbé
C
C
Maisse
G
G
D000081:Acetamides / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002397:Catfishes; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D019946:Propylene Glycol / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D000081:Acetamides; D003451:Cryoprotective Agents; D019946:Propylene Glycol; D000255:Adenosine Triphosphate; C013959:dimethylacetamide; D005990:Glycerol; D004121:Dimethyl Sulfoxide