Normalization of array hybridization experiments in differential gene expression analysis.
For detecting and confirming differentially expressed genes it is necessary to have a trustworthy reference. So called 'housekeeping genes' are frequently used for this purpose as internal standard. However, if the influence of new experimental conditions is to be analyzed it is not safe to assume a priori that the expression of these genes is not affected. Therefore two synthetic poly(A)-RNAs were generated by PCR and in vitro transcription. They were used as external standards for normalization of northern blots and cDNA arrays where non-regulated genes as internal reference were not available.
Eickhoff
B
B
Division of Experimental Immunopharmacology, Research Center Borstel, Parkallee 22, D-23845 Borstel, Germany. beickhof@fz-borstel.de
Korn
B
B
Schick
M
M
Poustka
A
A
van der Bosch
J
J
D000668:Ampicillin Resistance / Q000235:genetics; D015152:Blotting, Northern / Q000379:methods*; D018076:DNA, Complementary; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D006801:Humans; D007613:Kanamycin Resistance / Q000235:genetics; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012313:RNA / Q000138:chemical synthesis* / Q000378:metabolism; D012015:Reference Standards; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D012313:RNA