title: Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori.
abstract: A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.
authors.affiliation: Helicobacter Reference Unit, Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
authors.forename: J R
authors.initials: JR
authors.lastname: Gibson
authors.forename: N A
authors.initials: NA
authors.lastname: Saunders
authors.forename: B
authors.initials: B
authors.lastname: Burke
authors.forename: R J
authors.initials: RJ
authors.lastname: Owen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D017291:Clarithromycin / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000188:drug therapy / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D017931:DNA Primers; D017291:Clarithromycin