Analysis of genomic damage in the mutagen-sensitive mus-201 mutant of Drosophila melanogaster by arbitrarily primed PCR (AP-PCR) fingerprinting.
DNA repair mechanisms are important to maintain the stability of the genome. In Drosophila melanogaster, the mus-201 gene is required in the excision repair process. To study the contribution of the mus-201 gene in the stability of the Drosophila genome, we have used the arbitrarily primed PCR fingerprinting method (AP-PCR). We have analysed the changes in the genomic DNA fingerprints from the progeny of wild-type males crossed with mus-201 repair-deficient or repair-proficient females. After induction of DNA damage with 2-acetylaminofluorene (2-AAF) in the wild-type parental males, quantitative and qualitative differences in the AP-PCR fingerprints were detected between the two crosses, and the estimate of the genomic damage detected by AP-PCR has clearly shown that the mus-201 repair deficiency is associated with an increase of genomic damage. The predominant type of alterations detected by AP-PCR under the mus-201 repair-deficient conditions agree with the results obtained in microsatellite PCR analysis, suggesting that the role of the mus-201 gene, necessary in excision repair, is not associated to the mismatch repair process. The work reported here demonstrates that the AP-PCR is a suitable technique to analyse genetic alterations in D. melanogaster and, consequently, can be used to compare the susceptibility to genomic damage of different DNA repair mutants.
López
A
A
Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Xamena
N
N
Cabré
O
O
Creus
A
A
Marcos
R
R
Velázquez
A
A
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000818:Animals; D001483:Base Sequence; D004249:DNA Damage; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000235:genetics*; D018895:Microsatellite Repeats; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D009153:Mutagens; D015073:2-Acetylaminofluorene