Autoradiographic evidence that intrastriatal administration of adenosine A(1) receptor antisense oligodeoxynucleotide decreases adenosine A(1) receptors in the rat striatum and cortex.
In this study, the effect of a phosphorothioated A(1) adenosine receptor antisense oligodeoxynucleotide on A(1) receptor density and mRNA in the striatum and cortex of rats was determined. Receptor autoradiography and in situ hybridization revealed a reduction in striatal and cortical A(1) receptor density and cortical A(1) receptor mRNA, respectively, in antisense-treated brains but not in those treated with a mismatch oligonucleotide. There was no change in A(2) receptor binding. These data imply that the corticostriatal pathway synthesizes A(1) receptors and transports them to its terminals.
Phan
T A
TA
Department of Pharmacology, School of Medicine, East Carolina University, Greenville, NC 27858, USA.
Gray
A M
AM
Nyce
J W
JW
McGinty
J F
JF
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D002540:Cerebral Cortex / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D003864:Depression, Chemical; D004058:Diffusion; D005786:Gene Expression Regulation / Q000187:drug effects*; D017403:In Situ Hybridization; D007267:Injections; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018047:Receptors, Purinergic P1 / Q000096:biosynthesis / Q000235:genetics*; D013873:Thionucleotides / Q000494:pharmacology*
D009419:Nerve Tissue Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D018047:Receptors, Purinergic P1; D013873:Thionucleotides; D000241:Adenosine