Liver and kidney peroxisomes in lactating rats and their pups after treatment with ciprofibrate. Biochemical and morphometric analysis.
We administered the hypolipidemic drug ciprofibrate to lactating rats and examined the enzymatic content and ultrastructural features of liver and kidney peroxisomes, both in treated animals and in their pups. The peroxisomal morphometric parameters, in particular, were measured in specimens submitted to the cytochemical reaction for the marker enzyme catalase. In liver of treated rats, the activities of peroxisomal enzymes involved in the fatty acid catabolism were significantly increased, while D-amino acid oxidase activity was lower than in controls; increments were also found in relative volume and pleiomorphism degree of the peroxisomal compartment, where a catalase dilution was supposed to occur. In the kidney, the treatment induced generalized increases of all examined enzymes; values significantly higher than controls were found in peroxisomal relative volume and numerical density, while the peroxisomal mean diameter practically did not change. The two organs, moreover, were affected by the drug in an age-dependent way, the pups being more responsive than the adults. The organ- and age-specific responses to the drug are interpreted as possibly related to the tissue-specific distribution of the peroxisomal proliferator activated receptor isotypes.
Stefanini
S
S
Department of Cellular and Developmental Biology, University of Rome La Sapienza, Italy. stefanin@axcasp.caspur.it
Nardacci
R
R
Farioli-Vecchioli
S
S
Pajalunga
D
D
Sartori
C
C
D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D015415:Biomarkers; D002374:Catalase / Q000032:analysis; D002995:Clofibric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004798:Enzymes / Q000032:analysis; D005260:Female; D058607:Fibric Acids; D006651:Histocytochemistry; D007668:Kidney / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure*; D007774:Lactation / Q000502:physiology; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D009929:Organ Size; D020025:Peroxisome Proliferators / Q000494:pharmacology; D020675:Peroxisomes / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344
D015415:Biomarkers; D004798:Enzymes; D058607:Fibric Acids; D020025:Peroxisome Proliferators; D002995:Clofibric Acid; D002374:Catalase; C019304:ciprofibrate