Mannosylphosphodolichol synthase activity is associated with a 32 kDa phosphoprotein.
Failure of actinomycin D to block the activation of Dol-P-Man synthase in isoproterenol-treated capillary endothelial cells, supported that isoproterenol effect was not mediated by active transcription of the Dol-P-Man synthase gene during a short-term beta-adrenoreceptor stimulation. Instead, it was a net effect of protein phosphorylation by cAMP-dependent protein kinase. Using antibody as a probe we have now demonstrated that Dol-P-Man synthase activity is associated with a 32 kDa ER phosphoprotein.
Banerjee
D K
DK
Department of Biochemistry, School of Medicine, University of Puerto Rico, San Juan 00936-5067, USA. d.banerjee@remaca.upr.ciu.edu
DaSilva
J J
JJ
Bigio
B
B
D000313:Adrenal Medulla / Q000166:cytology / Q000201:enzymology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D007545:Isoproterenol / Q000494:pharmacology; D008364:Mannosyltransferases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation
D000318:Adrenergic beta-Agonists; D007106:Immune Sera; D010750:Phosphoproteins; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase; D007545:Isoproterenol