Effect of experimental diabetes and growth hormone administration on the strength of colonic anastomoses in rats.
The influence of growth hormone (GH; 2 mg/kg/day) administration on the mechanical breaking strength of colonic anastomoses in diabetic rats has been investigated on the day of operation (suture binding capacity) and after 4 and 7 days of healing. In diabetic rats, the suture binding capacity was decreased by 26% in both ultimate load and relative failure energy. After 4 days of healing, no difference was observed between control and diabetic animals. After 7 days, relative failure energy in the diabetic animals was reduced by 33%. GH administration to diabetic animals did not alter strength during the first week of healing. We found an increased circumference (33%) and defatted dry weight (22%) of the colon in diabetic rats. In conclusion, diabetes impairs the suture-binding capacity of the colon in rats, while there is only little influence on healing in the following week. GH administration could not influence the strength of colonic anastomoses in diabetic animals.
Seyer-Hansen
M
M
Department of Connective Tissue Biology, Institute of Anatomy, University of Aarhus, Denmark. msh@ana.au.dk
Andreassen
T T
TT
Christensen
H
H
Oxlund
H
H
D000714:Anastomosis, Surgical; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000503:physiopathology* / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013529:Surgical Wound Dehiscence / Q000517:prevention & control
D001786:Blood Glucose; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D006909:Hydroxyproline