As Chlamydomonas reinhardtii acclimates to low-CO2 conditions there is an increase in cyclophilin expression.
When exposed to low CO2 levels, Chlamydomonas reinhardtii acquires the ability to accumulate CO2 to increase photosynthetic carbon fixation. A cDNA library has been constructed and screened to facilitate the identification of the different genes and proteins involved in this acclimation to low-CO2 conditions. The differential cDNA library screening led to the identification of several cDNAs up-regulated under low-CO2 conditions. One such cDNA shows homology to cyclophilins, a class of immunophilins with a peptidyl-prolyl cis-trans isomerase activity. This is the first report of an algal cyclophilin. In this report we study the changes in the C. reinhardtii cyclophilin transcript and protein levels during low-CO2 adaptation. Possible reasons for the increased cyclophilin expression in response to the drop in CO2 concentration are discussed.
Somanchi
A
A
Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, USA.
Moroney
J V
JV
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002245:Carbon Dioxide / Q000494:pharmacology*; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase / Q000235:genetics*; D012313:RNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D018076:DNA, Complementary; D002245:Carbon Dioxide; D012313:RNA; D019696:Peptidylprolyl Isomerase