Birth of a healthy infant after conception with round spermatids isolated from cryopreserved testicular tissue.
OBJECTIVE
To report a case of nonobstructive azoospermia in which round spermatids recovered from thawed testicular tissue were used for injection.


METHODS
Case report.


METHODS
Reproductive Medicine Unit, S.I.S.ME.R.


METHODS
A 33-year-old azoospermic man.


METHODS
Intracytoplasmic sperm injection with frozen-thawed spermatids.


METHODS
Fertilization, embryo cleavage, pregnancy, and delivery.


RESULTS
Birth of a healthy, chromosomally normal girl.


CONCLUSIONS
Frozen-thawed testicular round spermatids from a patient with a history of incomplete spermatogenesis can maintain their viability and their capacity to fertilize and to lead to full-term pregnancy.
Gianaroli
L
L
Reproductive Medicine Unit, S.I.S.ME.R., Bologna, Italy. sismer@iol.it
Selman
H A
HA
Magli
M C
MC
Colpi
G
G
Fortini
D
D
Ferraretti
A P
AP
D000328:Adult; D001706:Biopsy; D002469:Cell Separation; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008845:Microinjections; D009845:Oligospermia / Q000473:pathology / Q000628:therapy*; D011247:Pregnancy; D013087:Spermatids / Q000502:physiology*; D013091:Spermatogenesis; D013737:Testis / Q000473:pathology*