Screening for abnormalities of chromosomes X, Y, and 18 and for diploidy in spermatozoa from infertile men participating in an in vitro fertilization-intracytoplasmic sperm injection program.
OBJECTIVE
To evaluate the frequency of disomy (for chromosomes X, Y, and 18) and of diploidy in the spermatozoa of infertile men undergoing intracytoplasmic sperm injection (ICSI).


METHODS
Prospective analysis of sperm nuclei by fluorescence in situ hybridization (FISH).


METHODS
University-affiliated IVF-ICSI program.


METHODS
Semen samples from 19 patients participating in an IVF-ICSI program.


METHODS
Semen samples were analyzed and prepared for FISH.


METHODS
Semen parameters were evaluated. The frequency of disomy for chromosomes X, Y, and 18 and the frequency of diploidy were analyzed by FISH.


RESULTS
A total of 9,373 spermatozoa from 19 infertile patients were analyzed and compared with spermatozoa from a control group of 5 healthy men. No differences in the frequency of disomy 18 were found, but statistically significant differences in the incidence of sex chromosome disomy and of diploidy were observed.


CONCLUSIONS
The study of sperm nuclei by FISH is useful to improve genetic counseling in infertile patients selected for ICSI.
Aran
B
B
Department of Obstetrics and Gynecology, Institut Universitari Dexeus, Barcelona, Spain.
Blanco
J
J
Vidal
F
F
Vendrell
J M
JM
Egozcue
S
S
Barri
P N
PN
Egozcue
J
J
Veiga
A
A
D000328:Adult; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D004171:Diploidy; D005307:Fertilization in Vitro; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012016:Reference Values; D020554:Sperm Injections, Intracytoplasmic; D014960:X Chromosome / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*