title: The effect of water, ascorbic acid, and cranberry derived supplementation on human urine and uropathogen adhesion to silicone rubber.
abstract: In this study, urine was collected from groups of volunteers following the consumption of water, ascorbic acid, or cranberry supplements. Only ascorbic acid intake consistently produced acidic urine. Photospectroscopy data indicated that increased water consumption produced urine with lower protein content. Surface tension measurements of the collected urine showed that both water and cranberry supplementation consistently produced urine with surface tensions higher than the control or urine collected following ascorbic acid intake. These urine samples were also employed to study uropathogen adhesion to silicone rubber in a parallel plate flow chamber. Urine obtained after ascorbic acid or cranberry supplementation reduced the initial deposition rates and numbers of adherent Escherichia coli and Enterococcus faecalis, but not Pseudomonas aeruginosa, Staphylococcus epidermidis, or Candida albicans. Conversely, urine obtained from subjects with increased water intake vastly increased the initial deposition rates and numbers of adherent E. coli and E. faecalis (P < 0.05).
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario Health Sciences Centre, London, Canada.
authors.forename: M B
authors.initials: MB
authors.lastname: Habash
authors.forename: H C
authors.initials: HC
authors.lastname: Van der Mei
authors.forename: H J
authors.initials: HJ
authors.lastname: Busscher
authors.forename: G
authors.initials: G
authors.lastname: Reid
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000494:pharmacology; D001422:Bacterial Adhesion; D001628:Beverages; D002176:Candida albicans / Q000502:physiology; D004326:Drinking; D013293:Enterococcus faecalis / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology; D005638:Fruit; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011550:Pseudomonas aeruginosa / Q000502:physiology; D012826:Silicone Elastomers; D013212:Staphylococcus epidermidis / Q000502:physiology; D014556:Urine
chemical_list: D012826:Silicone Elastomers; D001205:Ascorbic Acid