The effect of mitomycin C after long-term storage on human Tenon's fibroblast proliferation.
OBJECTIVE
To investigate the effect of mitomycin C (MMC) after long-term storage on proliferation of human Tenon's fibroblasts in vitro.


METHODS
Human Tenon's fibroblasts in tissue culture were exposed for 5 minutes to MMC (0.4 mg/mL) that was either freshly prepared or had been stored for as long as 18 months at either 4 degrees C or -20 degrees C. The MTT colorimetric assay was used to determine the inhibition of proliferation as measured indirectly by mitochondrial activity.


RESULTS
The inhibition rate was 88% using fresh MMC, and declined to a mean of 73% when using MMC that had been stored for as long as 18 months at 4 degrees C; this decrease was not statistically significant. The mean inhibition for MMC stored at -20 degrees C was 68%, and this was significantly less than inhibition with fresh MMC. Inhibition did not vary significantly with MMC after different storage times.


CONCLUSIONS
Mitomycin C continues to have strong in vitro antiproliferative effects when stored for as long as 18 months at 4 degrees C or -20 degrees C. A significant decline in potency compared with fresh MMC occurs when MMC is stored at -20 degrees C.
Hu
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Department of Ophthalmology, University of Washington School of Medicine, Seattle 98195-6485, USA.
Chen
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PP
Oda
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D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003080:Cold Temperature; D004356:Drug Storage; D005123:Eye / Q000166:cytology* / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D013778:Tetrazolium Salts; D013844:Thiazoles; D016685:Mitomycin; C022616:thiazolyl blue