Transcription level of operon ftsYEX and activity of promoter P1 of rpoH during the cell cycle in Escherichia coli.
In Escherichia coli, the genes of the main heat-shock proteins are under the control of the product of gene rpoH, protein sigma 32. The distal promoter P1 of rpoH is located within the terminator of the division operon ftsYEX which could imply some coupling between ftsYEX transcription termination, P1 transcription and cell division. To study the possibility of this coupling, the level of transcription of ftsYEX and the activity of promoter P1 of rpoH were examined in synchronous cultures. Results indicate that transcription of ftsYEX and of rpoH from P1 is continuous, suggesting that ftsYEX transcription termination and P1 activity are not coupled to the cell cycle.
Gómez-Eichelmann
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MC
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, D.F., México.
Helmstetter
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CE
D002453:Cell Cycle; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D012808:Sigma Factor; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; D015719:Single-Strand Specific DNA and RNA Endonucleases