Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus.
Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment-length polymorphism analysis using four restriction enzymes.
Kurosawa
K
K
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Ikeda
Y
Y
Miyazawa
T
T
Izumiya
Y
Y
Nishimura
Y
Y
Nakamura
K
K
Sato
E
E
Mikami
T
T
Kai
C
C
Takahashi
E
E
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral; D015683:Gene Products, gag / Q000235:genetics*; D016089:Immunodeficiency Virus, Feline / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D015683:Gene Products, gag