title: Phosphate induced developmental arrest of hamster two-cell embryos is associated with disrupted ionic homeostasis.
abstract: Culture of hamster embryos with 0.35 mM inorganic phosphate results in developmental arrest at the 2-cell stage. These arrested 2-cell embryos were found to have significantly elevated levels of both intracellular pH and intracellular free calcium. Culture of 2-cell embryos with both glucose and phosphate did not further alter intracellular ionic homeostasis. Developmental arrest of 2-cell embryos was dependent on the concentration of phosphate used. Culture with 1.25 microM phosphate did not alter development, while concentrations of 2.5 microM and 5.0 microM resulted in a percentage of embryos arresting development at the 2-cell stage. Analysis of intracellular levels of pH and calcium after culture with different phosphate concentrations revealed a significant negative correlation between intracellular calcium levels and development beyond the 2-cell stage. There was no correlation between the increase in intracellular pH and embryo development in the presence of phosphate. The increase in intracellular calcium levels after culture with phosphate appears to be derived from intracellular pools, as preventing the influx of extracellular calcium did not alter development beyond the 2-cell stage. Therefore, it is apparent that a disruption in ionic homeostasis is associated with developmental arrest of hamster embryos cultured with phosphate.
authors.affiliation: Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin.
authors.forename: M
authors.initials: M
authors.lastname: Lane
authors.forename: T E
authors.initials: TE
authors.lastname: Ludwig
authors.forename: B D
authors.initials: BD
authors.lastname: Bavister
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000187:drug effects*; D005947:Glucose / Q000493:pharmacokinetics / Q000494:pharmacology; D006706:Homeostasis / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D010084:Oxidation-Reduction / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology*
chemical_list: D007477:Ions; D010710:Phosphates; D005947:Glucose; D002118:Calcium