Molecular polymorphism of Kaposi's sarcoma-associated herpesvirus (Human herpesvirus 8) latent nuclear antigen: evidence for a large repertoire of viral genotypes and dual infection with different viral genotypes.
Molecular polymorphism was found in Kaposi's sarcoma-associated herpesvirus (KSHV) latent nuclear antigen (LNA), mapped to the internal repeat domain of the encoding orf73 gene, and used to develop a novel genotyping technique, KSHV LNA genotyping (KVNAtyping). KVNAtype was stable during latent and lytic viral replication in cell culture and in humans. Diverse KVNAtypes were identified in 43 specimens: 6 KSHV cell lines and 6 Kaposi's sarcoma (KS) and 4 primary effusion lymphoma (PEL) tumor samples from the United States, 15 KS tumor samples from Italy, and 12 KS tumor samples from Zambia. A single KVNAtype was detected in each of 41 specimens, and 2 KVNAtypes were detected in each of 2 KS specimens. Multifocal KS from 3 patients showed the same single KVNAtype at all sites in each patient. These results demonstrate a large repertoire of KSHV genotypes and suggest that the development of most KSs and PELs is associated with a single viral genotype.
Gao
S J
SJ
Division of Infectious Diseases, Dept. of Pediatrics, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284-7811, USA. gaos@uthscsa.edu
Zhang
Y J
YJ
Deng
J H
JH
Rabkin
C S
CS
Flore
O
O
Jenson
H B
HB
D000956:Antigens, Viral / Q000235:genetics*; D002460:Cell Line; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005838:Genotype; D019288:Herpesvirus 8, Human / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012514:Sarcoma, Kaposi / Q000821:virology*
D000956:Antigens, Viral; D004279:DNA, Viral; D009687:Nuclear Proteins; C109540:latency-associated nuclear antigen