title: Bordetella bronchiseptica-mediated cytotoxicity to macrophages is dependent on bvg-regulated factors, including pertactin.
abstract: The effect of Bordetella bronchiseptica infection on the viability of murine macrophage-like cells and on primary porcine alveolar macrophages was investigated. The bacterium was shown to be cytotoxic for both cell types, particularly where tight cell-to-cell contacts were established. In addition, bvg mutants were poorly cytotoxic for the eukaryotic cells, while a prn mutant was significantly less toxic than wild-type bacteria. B. bronchiseptica-mediated cytotoxicity was inhibited in the presence of cytochalasin D or cycloheximide, an inhibitor of microfilament-dependent phagocytosis or de novo eukaryotic protein synthesis, respectively. The mechanism of eukaryotic cell death was examined, and cell death was found to occur primarily through a necrotic pathway, although a small proportion of the population underwent apoptosis.
authors.affiliation: Department of Veterinary Pathology, University of Glasgow Veterinary School, Garscube Estate, Glasgow G61 1QH, Scotland.
authors.forename: C B
authors.initials: CB
authors.lastname: Forde
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Roberts
mesh_terms: D018829:Adhesins, Bacterial / Q000502:physiology; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D016950:Bordetella bronchiseptica / Q000472:pathogenicity*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D006388:Hemagglutinins / Q000502:physiology; D008264:Macrophages / Q000473:pathology*; D009876:Operon / Q000502:physiology*; D010566:Virulence Factors, Bordetella
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D015638:Cytochalasin D; C068929:pertactin; D003513:Cycloheximide