title: A lack of evidence for down-modulation of CD3 zeta expression in colorectal carcinoma and pregnancy using multiple detection methods.
abstract: Loss of the T cell receptor-associated CD3 zeta chain has been proposed as a possible mechanism of the acquired immunosuppression in both tumour-bearing hosts, and in symptomatic patients with HIV infection. However, other reports suggest that the zeta-chain loss may in part be caused by protease activity of contaminating phagocytes ex vivo. Using flow cytometry and Western blot analysis on highly purified T cells, and ensuring adequate addition of protease inhibitors, we have studied the expression of CD3zeta on peripheral blood T cells from patients with colorectal carcinoma, and compared these with normal controls, and pregnant donors, as a further example of an immunocompromised state. Immunohistochemistry was performed on tumour sections from patients with colorectal carcinoma to measure CD3zeta expression in tumour infiltrating T cells, and compared with normal mucosa and tonsil. Using these three approaches, our data provide no evidence for downregulation of CD3zeta chain expression either in colorectal carcinoma or pregnancy and suggest that this explanation is unlikely to fully account for the reduced T cell function associated with these conditions in all patients.
authors.affiliation: Immunopathology Unit, Glaxo Wellcome Medicines Research Centre, Stevenage, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Deakin
authors.forename: K
authors.initials: K
authors.lastname: Singh
authors.forename: J S
authors.initials: JS
authors.lastname: Crowe
authors.forename: J H
authors.initials: JH
authors.lastname: Ellis
authors.forename: A
authors.initials: A
authors.lastname: Dalgleish
authors.forename: R J
authors.initials: RJ
authors.lastname: Leicester
authors.forename: C J
authors.initials: CJ
authors.lastname: Finlayson
authors.forename: W F
authors.initials: WF
authors.lastname: Miles
authors.forename: P F
authors.initials: PF
authors.lastname: Life
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D017252:CD3 Complex; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology; D008875:Middle Aged; D011247:Pregnancy / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology
chemical_list: D017252:CD3 Complex; C115335:CD3E protein, human; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; C054979:antigen T cell receptor, zeta chain