Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level.
In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin-2, G3.IL-2. A maximum concentration of G3.IL-2 (11.25 g l(-1)) was achieved in the induced recombinant culture growing at the rate of 0.056 h(-1). A steep decrease in the expression level of G3.IL-2 was observed at the post-induction specific growth rates higher than its optimal value (0.056 h(-1)). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post-induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture.
Saraswat
V
V
Biochemical Process Engineering R.U., Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon, South Korea.
Kim
D Y
DY
Lee
J
J
Park
Y
Y
D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D015870:Gene Expression; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*
D011993:Recombinant Fusion Proteins