A focusing image probe for assessing neural activity in vivo.
We describe a compact, focusing image probe to record rapid optical changes from neural tissue. A gradient index (GRIN) lens served as a relay lens from tissue to a microscope objective which projected an image onto a CCD camera. The microscope objective and camera assembly was adjusted independently from the GRIN lens, allowing focus changes without disturbing the probe/tissue interface; firm contact minimized movement and specular reflectance. Fiber optics around the probe perimeter provided diffuse illumination from a 780 nm laser, or 660 and 560 nm light emitting diodes. To characterize depth-of-field, we imaged a black suture through increasing tissue thicknesses. Light modulation by the suture remained detectable down to 900 microm using 780 nm illumination. We acquired images from cardiorespiratory areas of the rat dorsal medulla, at different depths and illumination wavelengths. Images illuminated at 560 nm were dominated by vasculature flow patterns, while 660 nm illumination revealed different spatial patterns which preceded vascular flow by 40 ms and may represent cardiac-related neural activity. Using 780 nm light, image sequences triggered by the cardiac R-wave showed vascular perfusion changes with delayed and broader responses at deeper levels. Electrical stimulation within the vagal bundle caused fast optical changes which track the electrical response, with a different spatial distribution from hemodynamic signals.
Rector
D M
DM
Biophysics Group, Los Alamos National Laboratory, NM 87545, USA.
Rogers
R F
RF
George
J S
JS
D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D004558:Electric Stimulation; D006321:Heart / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D008027:Light; D018715:Microscopy, Video; D009474:Neurons / Q000502:physiology*; D051381:Rats; D012542:Scattering, Radiation; D014630:Vagus Nerve / Q000502:physiology