Trichinella spp.: differential expression of two genes in the muscle larva of encapsulating and nonencapsulating species.
Kuratli, S., Lindh, J. G., Gottstein, B., Smith, D. F., and Connolly, B. 1999. Trichinella spp.: Differential expression of two genes in the muscle larva of encapsulating and nonencapsulating species. Experimental Parasitology 93, 153-159. The expression of the two genes tsmyd-1 and tsJ5 was studied in the muscle stage larva of three different species of Trichinella. T. spiralis and T. britovi are both encapsulating species, while T. pseudospiralis is a nonencapsulating species. Expression of tsJ5 is developmentally regulated in T. spiralis and has been shown in this study to be down-regulated in the T. pseudospiralis muscle larva compared with the other two species. Immunoblot analysis has also revealed that the relative abundance of the protein product of this gene, TSJ5, is lower in T. pseudospiralis muscle larvae. It has previously been shown that expression of tsmyd-1 is not developmentally regulated in T. spiralis (Connolly et al. 1996). In contrast, expression of this gene is slightly increased in the muscle larvae of T. pseudospiralis. Southern analysis of genomic DNA from the three Trichinella species shows that both genes are highly conserved.
Kuratli
S
S
Institute of Parasitology, University of Bern, Länggass Strasse 122, Bern, CH-3012, Switzerland.
Lindh
J G
JG
Gottstein
B
B
Smith
D F
DF
Connolly
B
B
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017124:Conserved Sequence; D018509:DNA, Helminth; D015536:Down-Regulation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth / Q000235:genetics*; D007814:Larva / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000473:pathology; D017570:MyoD Protein / Q000235:genetics; D018510:RNA, Helminth / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014234:Trichinella / Q000235:genetics*
D018509:DNA, Helminth; D017570:MyoD Protein; D018510:RNA, Helminth