Rapid and simple micro-determination of carvedilol in rat plasma by high-performance liquid chromatography.
We studied the use of high-performance liquid chromatography (HPLC) with spectrofluorometric detection, using a solid-phase extraction for a simple, rapid and sensitive determination of plasma carvedilol levels in rats. Extracted aliquots were analyzed by HPLC, using a reversed-phase octadecyl silica column. The analytical mean recovery of carvedilol added to the blank plasma was 94.2%. The detection limit was 3.6 ng/ml in the plasma. The reproducibilities (C.V.) were 2.7-7.5% for the within-day assay, and 2.6-7.4% for the between-day assay, indicating that the method was effective for the determination of carvedilol plasma levels.
Hokama
N
N
Department of Hospital Pharmacy, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Hobara
N
N
Kameya
H
H
Ohshiro
S
S
Sakanashi
M
M
D000818:Animals; D002138:Calibration; D002227:Carbazoles / Q000032:analysis / Q000097:blood*; D000077261:Carvedilol; D002584:Cervix Uteri / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008297:Male; D011412:Propanolamines / Q000032:analysis / Q000097:blood*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D013623:Tail / Q000378:metabolism; D013997:Time Factors; D014665:Vasodilator Agents / Q000032:analysis / Q000097:blood*
D002227:Carbazoles; D011412:Propanolamines; D014665:Vasodilator Agents; D000077261:Carvedilol