title: The suppression of testis-brain RNA binding protein and kinesin heavy chain disrupts mRNA sorting in dendrites.
abstract: Ribonucleoprotein particles (RNPs) are thought to be key players in somato-dendritic sorting of mRNAs in CNS neurons and are implicated in activity-directed neuronal remodeling. Here, we use reporter constructs and gel mobility shift assays to show that the testis brain RNA-binding protein (TB-RBP) associates with mRNPs in a sequence (Y element) dependent manner. Using antisense oligonucleotides (anti-ODN), we demonstrate that blocking the TB-RBP Y element binding site disrupts and mis-localizes mRNPs containing (alpha)-calmodulin dependent kinase II (alpha)-CAMKII) and ligatin mRNAs. In addition, we show that suppression of kinesin heavy chain motor protein alters only the localization of (alpha)-CAMKII mRNA. Thus, differential sorting of mRNAs involves multiple mRNPs and selective motor proteins permitting localized mRNAs to utilize common mechanisms for shared steps.
authors.affiliation: Department of Physiology, Medical College of Virginia/Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Severt
authors.forename: T U
authors.initials: TU
authors.lastname: Biber
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: N B
authors.initials: NB
authors.lastname: Hecht
authors.forename: R J
authors.initials: RJ
authors.lastname: DeLorenzo
authors.forename: E R
authors.initials: ER
authors.lastname: Jakoi
mesh_terms: D000818:Animals; D016375:Antisense Elements (Genetics) / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis; D003712:Dendrites / Q000378:metabolism* / Q000502:physiology; D017930:Genes, Reporter / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D016547:Kinesins / Q000235:genetics*; D008565:Membrane Proteins / Q000032:analysis; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009924:Organ Culture Techniques; D017966:Pyramidal Cells / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics*; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D016375:Antisense Elements (Genetics); D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C490552:Strbp protein, rat; C013090:ligatin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D001616:beta-Galactosidase; D016547:Kinesins