Effects of green tea catechin on phospholipase A2 activity and antithrombus in streptozotocin diabetic rats.
The purpose of this study was to investigate the effects of dietary green tea catechin on phospholipase A2 (PLA2) activity and the antithrombotic reaction of platelets in streptozotocin (STZ)-diabetic rats. Sprague-Dawley male rats weighing 100 +/- 10 g were randomly divided into one normal and three STZ-diabetic groups, which were subdivided into catechin-free group (DM-0C), 0.5% catechin group (DM-0.5C) and 1% catechin group (DM-1C). The activity level of platelet phospholipase A2 was higher in the diabetic groups than in the normal group, while it was lower in DM-0.5C and DM-1C than in DM-0C. The activity of platelet cyclooxygenase in DM-0C was 1.1-fold as high as in the normal group, but was significantly reduced by catechin supplementation. The platelet thromboxane A2 (TXA2) formation became higher in DM-0C as compared to the normal group, but not in DM-0.5C and DM-1C. The synthesis of aortic prostacyclin (PGI2) was lower in DM-0C and DM-0.5C than in the normal group. The PGI2/TXA2 ratio was decreased to 55% in DM-0C, but was restored by catechin supplementation. These results indicate that STZ-diabetic rats are sensitive to platelet aggregation and thrombosis, and that the abnormality can be improved by dietary catechin.
Yang
J A
JA
Department of Food Science and Nutrition, Catholic University of Taegu-Hyosung, Kyungbuk, Korea.
Choi
J H
JH
Rhee
S J
SJ
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D002392:Catechin / Q000494:pharmacology* / Q000627:therapeutic use; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000150:complications; D011464:Epoprostenol / Q000096:biosynthesis; D008297:Male; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D010974:Platelet Aggregation; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control*; D013928:Thromboxane A2 / Q000097:blood; D013930:Thromboxane-A Synthase / Q000378:metabolism
D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D013928:Thromboxane A2; D002392:Catechin; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2; D013930:Thromboxane-A Synthase