Enhanced benzaldehyde formation by a monokaryotic strain of Pycnoporus cinnabarinus using a selective solid adsorbent in the culture medium.
A monokaryotic strain of the white-rot fungus Pycnoporus cinnabarinus was shown to produce, in a 2-L bioreactor culture, 100 mg.L-1 benzaldehyde (bitter almond aroma) from L-phenylalanine with a productivity of 33 mg.L-1.day-1. The addition of HP20 resin, a styrene divinylbenzene copolymer highly selective for benzaldehyde, enabled an eightfold increase in the production of benzaldehyde and a twofold increase in productivity. In the presence of HP20 resin, the production of 790 mg.L-1 benzaldehyde was concomitant with the synthesis of cinnamic acid derivatives of high organoleptic notes such as cinnamaldehyde, cinnamyl alcohol, and methyl cinnamate.
Lomascolo
A
A
Laboratoire de biotechnologie des champignons filamenteux, Institut national de la recherche agronomique (INRA), Ecole supérieure des ingénieurs de Luminy, Faculté des sciences de Luminy, Marseille, France.
Lesage-Meessen
L
L
Labat
M
M
Navarro
D
D
Delattre
M
M
Asther
M
M
D000327:Adsorption; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D003470:Culture Media; D010649:Phenylalanine / Q000378:metabolism*; D012116:Resins, Plant
D001547:Benzaldehydes; D003470:Culture Media; D012116:Resins, Plant; D010649:Phenylalanine; C032175:benzaldehyde