title: [Ultrastructural identification of Ehrlichia sp in an experimentally infected dog in Venezuela].
abstract: This study is the first report made in Venezuela concerning the ultrastructural characteristics of Ehrlichia sp in mononuclear blood cells from an experimentally infected dog. The animal developed clinical manifestations characteristic of the infection, and typical intracitoplasmic inclusion bodies were clearly seen in blood smears stained with modified Giemsa examined by light microscopy. Microorganisms were visualized by transmission electron microscopy. The cytoplasmic inclusions, consisted of membrane-lined vacuole-containing elementary bodies. The organisms were extremely pleomorphic. Elementary bodies were surrounded by two distinct membranes and each was constituted by electro-dense granules. These findings corresponded to the described electron microscopy morphology which characterizes the Ehrlichia genus.
authors.affiliation: Centro de Investigaciones Biomédicas (BIOMED), Facultad de Ciencias de la Salud, Universidad de Carabobo, Núcleo Aragua.
authors.forename: N
authors.initials: N
authors.lastname: Gutiérrez
authors.forename: M
authors.initials: M
authors.lastname: Martínez
authors.forename: C
authors.initials: C
authors.lastname: Arraga-Alvarado
authors.forename: A
authors.initials: A
authors.lastname: Bretaña
authors.forename: I
authors.initials: I
authors.lastname: Pacheco
authors.forename: G
authors.initials: G
authors.lastname: Comach
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004285:Dogs; D004536:Ehrlichia / Q000302:isolation & purification / Q000648:ultrastructure*; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D006400:Hematocrit; D002479:Inclusion Bodies / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D014617:Vacuoles / Q000648:ultrastructure; D014687:Venezuela