Cell wall-affecting antibiotics induce expression of a novel gene, drp35, in Staphylococcus aureus.
A novel gene, drp35, of Staphylococcus aureus, which was inducible especially with cell wall-affecting antibiotics, has been cloned. Analysis of differential hybridization with mRNAs enhanced in the presence of beta-lactams resulted in two positive clones that harbored a new gene encoding a 35,845-Da protein (Drp35) and the penicillin-binding protein 2 (PBP2). Immunoblot analysis revealed that the Drp35 protein band was evidently enhanced after 30 min in the presence of beta-lactams. The Drp35 expression was also enhanced with not only beta-lactams, but also vancomycin, bacitracin, and fosfomycin. Homology search revealed that Drp35 was a new protein. Our results revealed that it was specific in S. aureus and respondent to these agents in both methicillin-resistant and -sensitive strains of S. aureus.
Murakami
H
H
College of Medical Technology and Nursing, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan.
Matsumaru
H
H
Kanamori
M
M
Hayashi
H
H
Ohta
T
T
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002473:Cell Wall / Q000187:drug effects; D003001:Cloning, Molecular; D005578:Fosfomycin / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000235:genetics; D006867:Hydrolases; D016106:Methicillin Resistance; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D002267:Muramoylpentapeptide Carboxypeptidase; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology; D010458:Peptidyl Transferases / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C400364:drp35 protein, Staphylococcus aureus; D001414:Bacitracin; D005578:Fosfomycin; D014640:Vancomycin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D006867:Hydrolases; D002267:Muramoylpentapeptide Carboxypeptidase; D010068:Oxacillin