Nucleotide sequence of exon 2 to 4 of the R-ras gene in the hermaphroditic fish Rivulus marmoratus.
We have cloned the ras homologue from the mangrove rivulus (Rivulus marmoratus) after low-stringency plaque hybridization of the mangrove rivulus genomic DNA library. This mangrove rivulus ras homologue showed a 76% amino acid homology to the human R(related)-ras gene with identical exon/intron boundary and was named the mangrove rivulus R-ras gene. The mangrove rivulus R-ras gene spanned 1.8 kb and consisted of at least 5 exons. The exon/intron boundaries coincided with the rule of GT/AG of consensus splice acceptor and donor sequences. To our knowledge this is the first report that fish also have the R-ras gene with identical exon/intron boundaries and extensive homology with human.
Lee
J S
JS
Department of Virology/Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA. jae.lee@stjude.org
Park
E H
EH
Choe
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics*; D011905:Genes, ras; D057850:Hermaphroditic Organisms; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019849:Sex Determination Processes; D018631:ras Proteins / Q000737:chemistry / Q000235:genetics*
D020558:GTP Phosphohydrolases; C085929:RRAS protein, human; D018631:ras Proteins