title: Birth of a healthy infant after conception with round spermatids isolated from cryopreserved testicular tissue.
abstract: OBJECTIVE
  To report a case of nonobstructive azoospermia in which round spermatids recovered from thawed testicular tissue were used for injection.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Reproductive Medicine Unit, S.I.S.ME.R.
  
  
  METHODS
  A 33-year-old azoospermic man.
  
  
  METHODS
  Intracytoplasmic sperm injection with frozen-thawed spermatids.
  
  
  METHODS
  Fertilization, embryo cleavage, pregnancy, and delivery.
  
  
  RESULTS
  Birth of a healthy, chromosomally normal girl.
  
  
  CONCLUSIONS
  Frozen-thawed testicular round spermatids from a patient with a history of incomplete spermatogenesis can maintain their viability and their capacity to fertilize and to lead to full-term pregnancy.
authors.affiliation: Reproductive Medicine Unit, S.I.S.ME.R., Bologna, Italy. sismer@iol.it
authors.forename: L
authors.initials: L
authors.lastname: Gianaroli
authors.forename: H A
authors.initials: HA
authors.lastname: Selman
authors.forename: M C
authors.initials: MC
authors.lastname: Magli
authors.forename: G
authors.initials: G
authors.lastname: Colpi
authors.forename: D
authors.initials: D
authors.lastname: Fortini
authors.forename: A P
authors.initials: AP
authors.lastname: Ferraretti
mesh_terms: D000328:Adult; D001706:Biopsy; D002469:Cell Separation; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008845:Microinjections; D009845:Oligospermia / Q000473:pathology / Q000628:therapy*; D011247:Pregnancy; D013087:Spermatids / Q000502:physiology*; D013091:Spermatogenesis; D013737:Testis / Q000473:pathology*