An exploration of the accuracy of an invasive method for measuring the volume of specimens of Amoeba proteus.
A method is described for measuring the volume of individual specimens of Amoeba proteus which utilizes an easily constructed compressor to flatten the specimen to a known thickness. The microscopic image of the flattened specimen is captured on tape, digitized and analysed with the NIH Image software. The results from one specimen are given to illustrate the sources and magnitude of errors affecting these volume measurements.
Whalen
T A
TA
Biology Department, Siena College, 515 Loudon Road, Loudonville, NY 12211, USA. Whalen@Siena.edu
Demarco
A J
AJ
D000656:Amoeba / Q000166:cytology*; D000818:Animals; D048429:Cell Size; D007091:Image Processing, Computer-Assisted; D009316:National Institutes of Health (U.S.); D012984:Software; D014481:United States