title: T-cell recognition of Mycobacterium tuberculosis culture filtrate fractions in tuberculosis patients and their household contacts.
abstract: We examined the immune responses of patients with active pulmonary tuberculosis (TB) and their healthy household contacts to short-term culture filtrate (ST-CF) of Mycobacterium tuberculosis or molecular mass fractions derived from it. Our goal was to identify fractions strongly recognized by donors and differences among the donor groups of possible relevance for vaccine development. The study population consisted of 65 human immunodeficiency virus-negative donors from the Hossana Regional Hospital, Hossana, Ethiopia. Peripheral blood leukocytes from the donors were stimulated with different antigens and immune responses were determined. Household contacts produced significantly higher levels of gamma interferon (IFN-gamma) than the TB patients in response to antigens present in ST-CF and the 10 narrow-molecular-mass fractions. A similar difference in leukocyte proliferative responses to the antigens between the two groups was also found. In general, while all fractions stimulated immune responses, the highest activity was seen with the low-molecular-mass fractions, which include well-defined TB antigens such as ESAT-6. Leukocytes from contacts of TB patients with severe disease produced higher levels of antigen-specific IFN-gamma than those from contacts of patients with minimal disease. Both groups of contacts exhibited higher cell-mediated responses than the patients themselves. The enhanced immune response of healthy contacts, especially those of patients with severe disease, to secreted mycobacterial antigens is suggestive of an early stage of infection by M. tuberculosis, which could in time result in overt disease or containment of the infection. This possibility is currently being investigated by follow-up studies of the household contacts.
authors.affiliation: Armauer Hansen Research Institute, Addis Ababa, Ministry of Health, Hossana, Ethiopia.
authors.forename: A
authors.initials: A
authors.lastname: Demissie
authors.forename: P
authors.initials: P
authors.lastname: Ravn
authors.forename: J
authors.initials: J
authors.lastname: Olobo
authors.forename: T M
authors.initials: TM
authors.lastname: Doherty
authors.forename: T
authors.initials: T
authors.lastname: Eguale
authors.forename: M
authors.initials: M
authors.lastname: Geletu
authors.forename: W
authors.initials: W
authors.lastname: Hailu
authors.forename: P
authors.initials: P
authors.lastname: Andersen
authors.forename: S
authors.initials: S
authors.lastname: Britton
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D003470:Culture Media; D005374:Filtration; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D003470:Culture Media; D007371:Interferon-gamma