title: Separation and purification of isoflavones from Pueraria lobata by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) was applied to the semipreparative separation and purification of puerarin and related isoflavones from a crude extract of Pueraria lobata. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). Using the above solvent system the preparative HSCCC was successfully performed yielding six relatively pure isoflavones including puerarin from 80 mg of the crude extract in one-step separation.
authors.affiliation: Beijing Institute of New Technology Application, China.
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal