title: Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems.
abstract: The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both beta-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.
authors.affiliation: Department of Biology, University of York, UK.
authors.forename: N
authors.initials: N
authors.lastname: Boulter
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: G
authors.initials: G
authors.lastname: Wilkie
authors.forename: S
authors.initials: S
authors.lastname: Williamson
authors.forename: E
authors.initials: E
authors.lastname: Kirvar
authors.forename: P
authors.initials: P
authors.lastname: Knight
authors.forename: E
authors.initials: E
authors.lastname: Glass
authors.forename: J
authors.initials: J
authors.lastname: Campbell
authors.forename: S
authors.initials: S
authors.lastname: Morzaria
authors.forename: V
authors.initials: V
authors.lastname: Nene
authors.forename: A
authors.initials: A
authors.lastname: Musoke
authors.forename: C
authors.initials: C
authors.lastname: d'Oliveira
authors.forename: M J
authors.initials: MJ
authors.lastname: Gubbels
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
authors.forename: R
authors.initials: R
authors.lastname: Hall
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000494:pharmacology; D015800:Protozoan Proteins / Q000008:administration & dosage* / Q000494:pharmacology*; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D016796:Theileria annulata / Q000276:immunology*; D013801:Theileriasis / Q000517:prevention & control*
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; C091729:SPAG-1 antigen, Theileria annulata; D005620:Freund's Adjuvant