title: Localization and changes in superoxide dismutase immunoreactivity in rat pulp after tooth preparation.
abstract: OBJECTIVE
  To examine the distribution of superoxide in the uninflamed and inflamed dental pulp by characterizing the immunoreactivity of the detoxifying antioxidant enzymes, manganese and copper-zinc superoxide dismutases (MnSOD and CuZnSOD, respectively).
  
  
  METHODS
  In 12 rats, mesial cavity preparations were made on the maxillary right first molar; left molars were unoperated controls. After 5 days, the rats were killed, and histologic sections were processed by using MnSOD and CuZnSOD immunoreactivity, and the extent of inflammation was evaluated on alternate sections stained with hematoxylin and eosin.
  
  
  RESULTS
  In the hematoxylin and eosin-stained sections, inflammation was consistent with round-cells: macrophages, lymphocytes, and plasma cells that coalesced into a distinct leukocytic "lesion", which obliterated portions of the underlying pulp. Both MnSOD and CuZnSOD immunoreactivity increased dramatically in inflammatory cells within the leukocytic lesion and in the tissue surrounding the lesion.
  
  
  CONCLUSIONS
  The findings suggest that the protective role of SOD increases within pulp cells that are undergoing inflammatory stimulation. SOD immunoreactivity may be an early indicator of stress in pulp.
authors.affiliation: University of Michigan, Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, Ann Arbor 48109-1078, USA.
authors.forename: K R
authors.initials: KR
authors.lastname: Baumgardner
authors.forename: A S
authors.initials: AS
authors.lastname: Law
authors.forename: G F
authors.initials: GF
authors.lastname: Gebhart
mesh_terms: D000818:Animals; D003782:Dental Pulp / Q000201:enzymology* / Q000276:immunology; D007150:Immunohistochemistry; D011671:Pulpitis / Q000201:enzymology / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013482:Superoxide Dismutase / Q000276:immunology / Q000378:metabolism*; D019246:Tooth Preparation
chemical_list: D013482:Superoxide Dismutase