title: Indirect enzyme-linked immunosorbent assay for the identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
abstract: Polyclonal antibodies produced against soluble muscle protein extracts from sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific identification of fillets from these flatfish species. The assay was performed in two different formats: microtiter plates and immunostick tubes. Immunorecognition of antibodies adsorbed to their specific fish samples was made with goat antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of all flatfish species studied.
authors.affiliation: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. anaces@eucmax.sim.ucm.es
authors.forename: A
authors.initials: A
authors.lastname: Céspedes
authors.forename: T
authors.initials: T
authors.lastname: García
authors.forename: E
authors.initials: E
authors.lastname: Carrera
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: L
authors.initials: L
authors.lastname: Asensio
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005412:Flatfishes / Q000145:classification*; D005432:Flounder / Q000145:classification; D005511:Food Handling / Q000379:methods*; D009124:Muscle Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D009124:Muscle Proteins