Cloning and sequencing of the rat cDNAs encoding class I beta-tubulin.
Two kinds of rat cDNA clones encoding class I beta-tubulin were isolated from the neonatal rat brain, and sequenced. They corresponded to mRNA species formed by the alternative usage of polyadenylation signals. The determined nucleotide sequence showed high (84.6%) identity to rat class II beta-tubulin, the only rat beta-tubulin sequence reported in the nucleotide database, in the coding region, but relatively low (47.3-50.7%) in the noncoding region. The Northern blot analysis using the 3'-noncoding fragment as a probe showed that both mRNA species of the class I beta-tubulin were down-regulated during brain development.
Usui
H
H
Department of Molecular Neuropathology, Brain Research Institute, Niigata University, Asahimachi, Japan. usui@bri.niigata-u.ac.jp
Miyazaki
Y
Y
Xin
D
D
Ichikawa
T
T
Kumanishi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014404:Tubulin / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D014404:Tubulin