title: Detection of early developmental stages of Myxobolus cerebralis in fish and tubificid oligochaete hosts by in situ hybridization.
abstract: The myxosporean and actinosporean spores of Myxobolus cerebralis develop through many stages in their respective hosts, salmonid fishes and a tubificid oligochaete. Using a modified, non-radioactive in situ hybridization protocol, the parasite, which exhibits radically different structural forms during its development in each host, could be specifically detected in paraffin-embedded tissues of both fish and oligochaetes. Our study aims to demonstrate the application of the technique for detection of early stages of M. cerebralis in both hosts.
authors.affiliation: School of Veterinary Medicine, Department of Medicine and Epidemiology, University of California, Davis 95616, USA.
authors.forename: D B
authors.initials: DB
authors.lastname: Antonio
authors.forename: M
authors.initials: M
authors.lastname: El-Matbouli
authors.forename: R P
authors.initials: RP
authors.lastname: Hedrick
mesh_terms: D000818:Animals; D056890:Eukaryota / Q000254:growth & development* / Q000302:isolation & purification; D005393:Fish Diseases / Q000469:parasitology*; D006790:Host-Parasite Interactions; D017403:In Situ Hybridization; D009835:Oligochaeta / Q000469:parasitology*; D017686:Oncorhynchus mykiss / Q000469:parasitology*; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms