title: The Candida albicans phospholipomannan is a family of glycolipids presenting phosphoinositolmannosides with long linear chains of beta-1,2-linked mannose residues.
abstract: In a series of studies, we have shown that Candida albicans synthesizes a glycolipid, phospholipomannan (PLM), which reacted with antibodies specific for beta-1,2-oligomannosides and was biosynthetically labeled by [(3)H]mannose, [(3)H]palmitic acid, and [(32)P]phosphorus. PLM has also been shown to be released from the C. albicans cell wall and to bind to and stimulate macrophage cells. In this study, we show by thin layer chromatography scanning of metabolically radiolabeled extracts that the C. albicans PLM corresponds to a family of mannose and inositol co-labeled glycolipids. We describe the purification process of the molecule and the release of its glycan fraction through alkaline hydrolysis. Analysis of this glycan fraction by radiolabeling and methylation-methanolysis confirmed the presence of inositol and of 1, 2-linked mannose units. NMR studies evidenced linear chains of beta-1,2-oligomannose as the major PLM components. Mass spectrometry analysis revealed that these chains were present in phosphoinositolmannosides with degrees of polymerization varying from 8 to 18 sugar residues. The PLM appears as a new type of eukaryotic inositol-tagged glycolipid in relationship to both the absence of glucosamine and the organization of its glycan chains. This first structural evidence for the presence of beta-1, 2-oligomannosides in a glycoconjugate other than the C. albicans phosphopeptidomannan may have some pathophysiological relevance to the adhesive, protective epitope, and signaling properties thus far established for these residues.
authors.affiliation: Equipe Mixte de l'INSERM 99-15, Laboratoire de Mycologie Fondamentale et Appliquée, Faculté de Médecine, Centre Hospitalier Universitaire, Place de Verdun, 59045 Lille Cedex, France.
authors.forename: P A
authors.initials: PA
authors.lastname: Trinel
authors.forename: Y
authors.initials: Y
authors.lastname: Plancke
authors.forename: P
authors.initials: P
authors.lastname: Gerold
authors.forename: T
authors.initials: T
authors.lastname: Jouault
authors.forename: F
authors.initials: F
authors.lastname: Delplace
authors.forename: R T
authors.initials: RT
authors.lastname: Schwarz
authors.forename: G
authors.initials: G
authors.lastname: Strecker
authors.forename: D
authors.initials: D
authors.lastname: Poulain
mesh_terms: D002176:Candida albicans / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents; D007294:Inositol / Q000032:analysis; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011865:Radioisotope Dilution Technique; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014316:Tritium
chemical_list: D006017:Glycolipids; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; C400188:phospholipomannan; D014316:Tritium; D010758:Phosphorus; D019308:Palmitic Acid; D007294:Inositol; D008358:Mannose