Optical imaging reveals elevated intracellular chloride in hippocampal pyramidal neurons after oxidative stress.
The accumulation of reactive oxygen species (ROS) in the brain is associated with several neurodegenerative conditions. ROS can affect ionic homeostasis leading to impaired neurotransmission. Here, we determined the ability of H(2)O(2), a membrane permeant ROS, to alter intraneuronal Cl(-), an important regulator of neuronal excitability. Real-time alterations in intracellular chloride, [Cl(-)]i, were measured with UV laser scanning confocal microscopy in hippocampal slices loaded with the cell-permeant form of 6-methoxy-N-ethylquinolium iodide (MEQ), a Cl(-)-sensitive fluorescent probe. In slices superfused with H(2)O(2) for 10 min, there was a significant decrease in MEQ fluorescence (elevation in [Cl(-)]i) in area CA1 pyramidal cell soma but not in interneurons located in stratum radiatum. Alterations in [Cl(-)]i induced by H(2)O(2) were prevented by the iron chelator deferoxamine and the vitamin E analog Trolox, suggesting the involvement of free radicals. The influx of Cl(-) probably occurred through the GABA-gated Cl(-) channel because the effects of H(2)O(2) were blocked by picrotoxin. In addition, HPLC analysis of the superfusates indicated that GABA and glutamate accumulated extracellularly after H(2)O(2) exposure. Excitatory amino acid receptor antagonists 2-amino-5-phoshopentanoic acid and 1,2,3,4-tetrahydro-6-nitro-2, 3-dioxo-benzo[f]quinoxaline-7-sulfonamide also attenuated the effect of H(2)O(2) on MEQ fluorescence. The changes in [Cl(-)]i induced by H(2)O(2) were Ca(2+)-dependent and Na(+)-independent. After exposure of slices to H(2)O(2), the ability of the GABA agonist muscimol to increase [Cl(-)]i was attenuated. Thus, ROS, like H(2)O(2), may impair transmembrane Cl(-) gradients and reduce inhibitory neurotransmission, further promoting neuronal damage in oxidative stress-related disease and in aging.
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Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Schwartz-Bloom
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D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002463:Cell Membrane Permeability; D002712:Chlorides / Q000378:metabolism*; D002839:Chromans / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005456:Fluorescent Dyes; D018756:GABA Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D018613:Microscopy, Confocal / Q000379:methods; D009118:Muscimol / Q000494:pharmacology; D009557:Nipecotic Acids / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D017966:Pyramidal Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011806:Quinolinium Compounds; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000502:physiology; D000078308:Tiagabine; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000975:Antioxidants; D002712:Chlorides; D002839:Chromans; D005456:Fluorescent Dyes; D018756:GABA Antagonists; D009557:Nipecotic Acids; D011806:Quinolinium Compounds; D011810:Quinoxalines; D017382:Reactive Oxygen Species; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; C070311:6-methoxy-N-ethylquinolinium; D009118:Muscimol; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D015763:2-Amino-5-phosphonovalerate; D006861:Hydrogen Peroxide; D003676:Deferoxamine; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D000078308:Tiagabine