A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption.
A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp, designated CaFET3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70.5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55%. CaFET3 was localized on chromosome 6. A null mutant (fet3delta/fet3delta) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wild-type strain the fet3delta mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3delta mutant in iron-limited medium was compensated by transformation with CaFET3. The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.
Eck
Raimund
R
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1.
Hundt
Susanne
S
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1.
Härtl
Albert
A
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1.
Roemer
Ernst
E
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1.
Künkel
Waldemar
W
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1.
D000595:Amino Acid Sequence; D000818:Animals; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D002177:Candidiasis / Q000382:microbiology*; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism*; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006801:Humans; D007501:Iron / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
D003300:Copper; D007501:Iron; D010088:Oxidoreductases