Electrospray mass spectrometry and supercritical fluid chromatography of methylated beta-cyclodextrins.
Five commercial dimethylated beta-cyclodextrin (DM-beta-CD) samples were analysed by electrospray (or ionspray) mass spectrometry (ESI-MS) and supercritical fluid chromatography (SFC) with evaporative light scattering detection. A silica and a nitro-bonded silica were selected using CO2-methanol-acetonitrile-water and CO2-methanol as mobile phase, respectively. An extensive optimisation scheme was performed for mobile phase selection. Both SFC systems were used for analyses of complex DM-beta-CD samples. Peak identifications were made using off-line ESI-MS. Commercial DM-beta-CDs are impure mixtures of homologues and isomers and analysis reveals that every manufacturer produces a different mixture.
Salvador
A
A
Institut de Chimie Organique et Analytique (ICOA), UPRES A, Université d'Orléans, France.
Herbreteau
B
B
Dreux
M
M
D003505:Cyclodextrins / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex