Autoradiographic demonstration of quinuclidinyl benzilate binding sites in the vestibular organs of the gerbil.
Gerbil vestibular tissues were isolated by microdissection and incubated in vitro with 3H-quinuclidinyl benzilate (3H-QNB). Control tissues were incubated in medium containing unlabeled atropine to differentiate non-specific from specific binding. Autoradiographic grain densities were determined by morphometric techniques and evaluated by two-tailed t-test. The label densities of sensory epithelia from experimental preparations of ampulla, utricle and saccule were found to be significantly higher than those in the adjacent endolymphatic compartment and also higher than those of adjacent stromal tissue comprising connective tissue, nerve fibers and capillaries. In contrast, no tissue region in atropine controls showed label density significantly above that of the endolymphatic compartment. Label density of ampullar sensory epithelium incubated with 3H-QNB alone was significantly higher than that of sensory epithelium from utricle or saccule. Grain density was greater in the peripheral regions of the ampullar crista compared to the vertex. Appreciable label was also present in nerve bundles beneath the sensory epithelium of the ampulla. The current study demonstrates the existence of putative muscarinic neurotransmitter/neuromodulator receptor sites in mammalian vestibular sense organs at locations corresponding to efferent innervation, with particularly significant concentrations in the ampulla.
Drescher
D G
DG
Laboratory of Bio-otology, Department of Otolaryngology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA. ddresch@cmb.biosci.wayne.edu
Kerr
T P
TP
Drescher
M J
MJ
D000818:Animals; D001345:Autoradiography; D005849:Gerbillinae; D018069:Hair Cells, Vestibular / Q000737:chemistry* / Q000378:metabolism; D018727:Muscarinic Antagonists / Q000378:metabolism* / Q000494:pharmacology*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism* / Q000494:pharmacology*; D011869:Radioligand Assay; D012444:Saccule and Utricle / Q000166:cytology; D014316:Tritium; D014722:Vestibule, Labyrinth / Q000166:cytology
D018727:Muscarinic Antagonists; D014316:Tritium; D011813:Quinuclidinyl Benzilate