title: DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.
abstract: We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold.
authors.affiliation: Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK.
authors.forename: M D
authors.initials: MD
authors.lastname: Keppler
authors.forename: C M
authors.initials: CM
authors.lastname: McKeen
authors.forename: O
authors.initials: O
authors.lastname: Zegrocka
authors.forename: L
authors.initials: L
authors.lastname: Strekowski
authors.forename: T
authors.initials: T
authors.lastname: Brown
authors.forename: K R
authors.initials: KR
authors.lastname: Fox
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I; D008024:Ligands; D009690:Nucleic Acid Conformation; D011804:Quinolines / Q000737:chemistry*
chemical_list: D008024:Ligands; D011804:Quinolines; C102232:naphthylquinoline triple-helix-binding ligand; D004247:DNA; D003850:Deoxyribonuclease I