SPARC regulates the expression of collagen type I and transforming growth factor-beta1 in mesangial cells.
The matricellular protein SPARC is expressed at high levels in cells that participate in tissue remodeling and is thought to regulate mesangial cell proliferation and extracellular matrix production in the kidney glomerulus in a rat model of glomerulonephritis (Pichler, R. H., Bassuk, J. A., Hugo, C., Reed, M. J., Eng, E., Gordon, K. L., Pippin, J., Alpers, C. E., Couser, W. G., Sage, E. H., and Johnson, R. J. (1997) Am. J. Pathol. 148, 1153-1167). A potential mechanism by which SPARC controls both cell cycle and matrix production has been attributed to its regulation of a pleiotropic growth factor. In this study we used primary mesangial cell cultures from wild-type mice and from mice with a targeted disruption of the SPARC gene. SPARC-null cells displayed diminished expression of collagen type I mRNA and protein, relative to wild-type cells, by the criteria of immunocytochemistry, immunoblotting, and the reverse transcription-polymerase chain reaction. The SPARC-null cells also showed significantly decreased steady-state levels of transforming growth factor-beta1 (TGF-beta1) mRNA and secreted TGF-beta1 protein. Addition of recombinant SPARC to SPARC-null cells restored the expression of collagen type I mRNA to 70% and TGF-beta1 mRNA to 100% of wild-type levels. We conclude that SPARC regulates the expression of collagen type I and TGF-beta1 in kidney mesangial cells. Since increased mitosis and matrix deposition by mesangial cells are characteristics of glomerulopathies, we propose that SPARC is one of the factors that maintains the balance between cell proliferation and matrix production in the glomerulus.
Francki
A
A
Department of Biological Structure, University of Washington, Seattle, Washington 98195-7420, USA.
Bradshaw
A D
AD
Bassuk
J A
JA
Howe
C C
CC
Couser
W G
WG
Sage
E H
EH
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics*; D005246:Feedback; D005786:Gene Expression Regulation; D005920:Glomerular Mesangium / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015676:Osteonectin / Q000502:physiology*; D016133:Polymerase Chain Reaction; D051381:Rats; D016212:Transforming Growth Factor beta / Q000235:genetics*
D015676:Osteonectin; D016212:Transforming Growth Factor beta; D003094:Collagen