Pyruvate flux distribution in NADH-oxidase-overproducing Lactococcus lactis strain as a function of culture conditions.
The influence of growth conditions on product formation from glucose by Lactococcus lactis strain NZ9800 engineered for NADH-oxidase overproduction was examined. In aerobic batch cultures, a large production of acetoin and diacetyl was found at acidic pH under pH-unregulated conditions. However, pyruvate flux was mainly driven towards lactate production when these cells were grown under strictly pH-controlled conditions. A decreased NADH-oxidase overproduction accompanied the homolactic fermentation, suggesting that the cellular energy was used with preference to maintain cellular homeostasis rather than for NADH-oxidase overproduction. The end product formation and NADH-oxidase activity were also studied in cells grown in aerobic continuous cultures under acidic conditions. A homoacetic type of fermentation as well as a low NADH-oxidase overproduction were observed at low dilution rates. NADH-oxidase was efficiently overproduced as the dilution rate was increased and consequently metabolic flux through lactate dehydrogenase drastically decreased. Under these conditions the flux limitation via pyruvate dehydrogenase was relieved and this enzymatic complex accommodated most of the pyruvate flux. Pyruvate was also significantly converted to acetoin and diacetyl via alpha-acetolactate synthase. At higher dilution rates, acetate production declined and the cultures turned to mixed-acid fermentation. These results suggest that the need to maintain the cellular homeostasis influenced NADH-oxidase overproduction and consequently the end product formation from glucose in these engineered strains.
Lopez de Felipe
F
F
Wageningen Center for Food Sciences, NIZO Food Research, P.O. Box 20, 6710 BA, Ede, The Netherlands. fxlopez@if.csic.es
Hugenholtz
J
J
D000332:Aerobiosis; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000096:biosynthesis*; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D009561:Nisin / Q000494:pharmacology; D019289:Pyruvic Acid / Q000378:metabolism*
D009097:Multienzyme Complexes; D009561:Nisin; D019289:Pyruvic Acid; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases