The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins.
The RGD1 gene, identified during sequencing of the Saccharomyces cerevisiae genome, encodes a protein with a Rho-GTPase activating protein (GAP) domain at the carboxy-terminal end. The Rgd1 protein showed two-hybrid interactions with the activated forms of Rho2p, Rho3p and Rho4p. Using in vitro assays, we demonstrated that Rgd1p stimulated the GTPase activity of both Rho3p and Rho4p; no stimulation was observed on Rho2p. In addition, the rho3Deltargd1Delta double mutant exhibited a dramatic growth defect compared to the single mutants, suggesting that Rgd1p has a GAP activity in vivo. The present study allowed the identification of the first GAP of Rho3p and Rho4p.
Doignon
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F
Laboratoire de Biologie Moléculaire et de Séquençage, UPR CNRS 9026, BP Box 64, 146 rue Léo Saignat, 33076, Bordeaux, France.
Weinachter
C
C
Roumanie
O
O
Crouzet
M
M
D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; D029701:Saccharomyces cerevisiae Proteins; C085379:rho GTPase-activating protein; D019204:GTP-Binding Proteins; C074647:RHO3 protein, S cerevisiae; C074648:RHO4 protein, S cerevisiae; D020741:rho GTP-Binding Proteins