Artefactual pyruvate and 2-oxobutyrate produced by trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of oxygen.
Trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of headspace atmospheric oxygen is shown to produce the trimethylsilyl derivatives of pyruvic and 2-oxobutyric acids, along with 2-hydroxy-2-methylmalonic and 2-hydroxy-2-ethylmalonic acids, respectively. This may lead to overestimation of these keto acids, if they were not oximated in the original sample, and the mistaken reporting of the 2-hydroxymalonates.
Mamer
O A
OA
Biomedical Mass Spectrometry Unit, McGill University, Montréal, Québec, Canada.
Choiniere
L
L
Boismenu
D
D
Lepine
F
F
D016477:Artifacts; D002087:Butyrates / Q000378:metabolism*; D008314:Malonates / Q000378:metabolism*; D008764:Methylmalonic Acid / Q000378:metabolism*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D014297:Trimethylsilyl Compounds / Q000378:metabolism
D002087:Butyrates; D008314:Malonates; D011773:Pyruvates; D014297:Trimethylsilyl Compounds; C038080:ethylmalonic acid; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; C005087:alpha-ketobutyric acid; D008764:Methylmalonic Acid; D010100:Oxygen