Presenilin 1 protein directly interacts with Bcl-2.
Presenilin proteins are involved in familial Alzheimer's disease, a neurodegenerative disorder characterized by massive death of neurons. We describe a direct interaction between presenilin 1 (PS1) and Bcl-2, a key factor in the regulation of apoptosis, by yeast two-hybrid interaction system, by co-immunoprecipitation, and by cross-linking experiments. Our data show that PS1 and Bcl-2 assemble into a macromolecular complex, and that they are released from this complex in response to an apoptotic stimulus induced by staurosporine. The results support the idea of cross-talk between these two proteins during apoptosis.
Alberici
A
A
Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Centro S. Giovanni di Dio, Neurobiology Laboratory, Alzheimer's Disease Unit, Via Pilastroni 4, 25123 Brescia, Italy.
Moratto
D
D
Benussi
L
L
Gasparini
L
L
Ghidoni
R
R
Gatta
L B
LB
Finazzi
D
D
Frisoni
G B
GB
Trabucchi
M
M
Growdon
J H
JH
Nitsch
R M
RM
Binetti
G
G
D000544:Alzheimer Disease / Q000503:physiopathology; D002460:Cell Line; D003001:Cloning, Molecular; D006367:HeLa Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D008214:Lymphocytes; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D053764:Presenilin-1; D011485:Protein Binding; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019311:Staurosporine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D019311:Staurosporine