title: Enhanced monomer-monomer interactions can suppress the recombination deficiency of the recA142 allele.
abstract: The RecA142 protein, in which valine is substituted for isoleucine-225, is defective for genetic recombination in vivo and for DNA strand exchange activity in vitro under conventional growth and reaction conditions respectively. However, we show that mildly acidic conditions restore both the in vitro DNA strand exchange activity and the in vivo function of RecA142 protein, suggesting that recombination function can be restored by a slight change in protein structure elicited by protonation. Indeed, we identified an intragenic suppressor of the recombination deficiency of the recA142 allele. This suppressor mutation is a substitution of leucine for glutamine at position 124. Based on the three-dimensional structure, the Q-124L substitution is predicted to make a new monomer-monomer contact with residue phenylalanine-21 of the adjacent RecA monomer. The Q-124L mutation is not allele specific, because it also suppresses the recombination deficiency of a recA deletion (Delta9), lacking nine amino acids at the amino-terminus, presumably by reinforcing the monomer-monomer interactions that are attenuated by the Delta9 deletion. Expression of RecA(Q-124L) protein is toxic to Escherichia coli, presumably because of enhanced affinity for DNA. We speculate as to how enhanced monomer-monomer interactions and acidic pH conditions can restore the recombination activity of some defective recA alleles.
authors.affiliation: Division of Biological Sciences, Sections of Microbiology and of Molecular and Cell Biology, University of California, Davis, CA 95616-8665, USA.
authors.forename: E N
authors.initials: EN
authors.lastname: Zaitsev
authors.forename: S C
authors.initials: SC
authors.lastname: Kowalczykowski
mesh_terms: D000143:Acids; D000483:Alleles; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007930:Leucine; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D011938:Rec A Recombinases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D013489:Suppression, Genetic
chemical_list: D000143:Acids; D004247:DNA; D011938:Rec A Recombinases; D007930:Leucine