Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus.
The gene encoding factor C (facC), an extracellular signal protein involved in cellular differentiation, was cloned from Streptomyces griseus 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34,523 Da. The mature extracellular 286 amino acid protein (31,038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected facC in several other Streptomyces strains, but database searches failed to identify a protein with significant homology to factor C. Expression of facC from a low-copy-number vector in S. griseus 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.
Birkó
Zsuzsa
Z
Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
Sümegi
Andrea
A
Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
Vinnai
Andrea
A
Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
van Wezel
Gilles
G
Department of Biochemistry, Leiden University, Gorlaeus Laboratory, PO Box 9502, 2300 RA Leiden, The Netherlands3.
Szeszák
Ferenc
F
Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
Vitális
Sándor
S
Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
Szabó
Pál T
PT
Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary2.
Kele
Zoltán
Z
Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary2.
Janáky
Tamás
T
Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary2.
Biró
Sándor
S
Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary1.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013305:Streptomyces griseus / Q000737:chemistry / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*
D001426:Bacterial Proteins; C402566:factor C, Streptomyces