title: Structure-based conformational preferences of amino acids.
abstract: Proteins can be very tolerant to amino acid substitution, even within their core. Understanding the factors responsible for this behavior is of critical importance for protein engineering and design. Mutations in proteins have been quantified in terms of the changes in stability they induce. For example, guest residues in specific secondary structures have been used as probes of conformational preferences of amino acids, yielding propensity scales. Predicting these amino acid propensities would be a good test of any new potential energy functions used to mimic protein stability. We have recently developed a protein design procedure that optimizes whole sequences for a given target conformation based on the knowledge of the template backbone and on a semiempirical potential energy function. This energy function is purely physical, including steric interactions based on a Lennard-Jones potential, electrostatics based on a Coulomb potential, and hydrophobicity in the form of an environment free energy based on accessible surface area and interatomic contact areas. Sequences designed by this procedure for 10 different proteins were analyzed to extract conformational preferences for amino acids. The resulting structure-based propensity scales show significant agreements with experimental propensity scale values, both for alpha-helices and beta-sheets. These results indicate that amino acid conformational preferences are a natural consequence of the potential energy we use. This confirms the accuracy of our potential and indicates that such preferences should not be added as a design criterion.
authors.affiliation: Department of Structural Biology, Fairchild Building, Stanford University, Stanford, CA 94305, USA. koehl@hyper.stanford.edu
authors.forename: P
authors.initials: P
authors.lastname: Koehl
authors.forename: M
authors.initials: M
authors.lastname: Levitt
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D000596:Amino Acids