Evidence for subnucleus interpolaris in craniofacial muscle pain mechanisms demonstrated by intramuscular injections with hypertonic saline.
The subnucleus interpolaris (Vi) has been identified as a major recipient for trigeminal ganglionic input from jaw muscles, and contains neurons with nociceptive properties similar to those in the subnucleus caudalis (Vc). Therefore, Vi may be another important site for processing craniofacial muscle nociception. The aims of present study were to define functional properties of Vi neurons that receive input from masseter muscle afferents by characterizing their responses to electrical, mechanical, and to chemical stimulation of the muscle. Ninety cells were identified as masseter muscle units in 11 adult cats. Most of these units (79%) received additional inputs from orofacial skin. Following the intramuscular injection of 5% hypertonic saline, 49% of the cells showed a significant modulation of either the resting discharge and/or responses to innocuous mechanical stimulation on their cutaneous receptive fields (RFs). The most common response to saline injection was an induction or facilitation of resting discharge which declined as an exponential decay function, returning to pre-injection level within 3-4 min. Forty-five percent of the muscle units that were tested with mechanical stimulation (13/29) showed a prolonged inhibition of mechanically-evoked responses. In most cases (8/13), the inhibitory response was accompanied by initial facilitation. The observations that Vi contained a population of neurons that receive small diameter muscle afferent inputs, responded to noxious mechanical stimulation on the muscle and to a chemical irritant that is known to produce pain in humans provide compelling evidence for the involvement of Vi in craniofacial muscle pain mechanisms.
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Department of Oral and Craniofacial Biological Sciences, University of Maryland at Baltimore School of Dentistry, 666 W. Baltimore Street, Baltimore, MD 21201, USA.
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D000818:Animals; D002415:Cats; D004558:Electric Stimulation; D004594:Electrophysiology; D007273:Injections, Intramuscular; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D009209:Myofascial Pain Syndromes / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D009619:Nociceptors / Q000502:physiology; D010812:Physical Stimulation; D020671:Posterior Horn Cells / Q000473:pathology / Q000502:physiology; D012462:Saline Solution, Hypertonic; D012867:Skin / Q000187:drug effects / Q000294:innervation; D013238:Stereotaxic Techniques; D013268:Stimulation, Chemical; D014278:Trigeminal Nuclei / Q000473:pathology / Q000503:physiopathology*
D012462:Saline Solution, Hypertonic