The complete cDNA sequence encoding dog gastric lipase.
Oligodeoxyribonucleotide ligation to single-stranded cDNA (SLIC) and polymerase chain reaction (PCR) techniques were used to clone an entire dog gastric lipase (DGL) cDNA. The size of the cDNA is confirmed by Northern blot analysis. The DGL is synthesized as a 379-amino acid mature polypeptide with a molecular mass of 43176 Da which is preceded by a 19-amino acid signal sequence located at the NH2-terminus. Comparison of the signal sequences reveals a high degree of similitude between the DGL, the human gastric lipase (HGL), the rabbit gastric lipase (RGL) and the rat lingual lipase (RLL).
Vaganay
S
S
Ecole Normale Supérieure de Cachan, Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Cachan, France.
Joliff
G
G
Bertaux
O
O
Toselli
E
E
Devignes
M D
MD
Bénicourt
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D004285:Dogs / Q000235:genetics*; D005753:Gastric Mucosa / Q000201:enzymology*; D006801:Humans; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D008049:Lipase