title: Understanding of peak deterioration in hyphenated speciation systems due to gas-liquid separation in the hydride generation interface.
abstract: In hyphenated speciation systems with a hydride generation interface one of the processes influencing peak deterioration is gas-liquid separation. A mathematical model was developed to calculate attenuation, signal tailing and resolution loss of HPLC peaks due to gas-liquid separation. It was shown experimentally--using an HPLC-hydride generation-atomic fluorescence spectrometry system for arsenic speciation--that the mathematical model predicts peak deterioration well. This allowed us to study the parameters influencing the deterioration, viz. gas-liquid separation parameters (gas-liquid separator head space volume and purge gas volume flow-rate) and HPLC peak parameters [width (ratio) and resolution] theoretically, simulating HPLC peaks with gaussian functions.
authors.affiliation: Department of Radiochemistry, Delft University of Technology, The Netherlands. elteren@iri.tudelft.nl
authors.forename: J T
authors.initials: JT
authors.lastname: van Elteren
authors.forename: Z
authors.initials: Z
authors.lastname: Slejkovec
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005740:Gases / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D008956:Models, Chemical; D013050:Spectrometry, Fluorescence
chemical_list: D005740:Gases; D006859:Hydrogen