Interferon-gamma increases CD62L expression on human eosinophils.
BACKGROUND
L-selectin (CD62L) and Mac-1 (CD11b/CD18) play a crucial role in the infiltration of eosinophils. However, changes in CD62L and CD11b expression on eosinophils after stimulation with cytokines were little studied.


METHODS
Eosinophils in peripheral blood of healthy volunteers were purified and cultured with interleukin-5 (IL-5), granulocyte/macrophage colony-stimulating factor (GM-CSF) or interferon-gamma (IFN-gamma). After up to 24 h incubation, CD62L and CD11b expression were examined using flow cytometry. The effects of dexamethasone (Dex), cycloheximide (CHX) or theophylline on CD62L expression on IFN-gamma-stimulated eosinophils were also studied.


RESULTS
IL-5 or GM-CSF downregulated CD62L and upregulated CD11b expression on eosinophils after 30 min stimulation. Conversely, IFN-gamma upregulated CD62L after 12 h stimulation in a time- and dose-dependent manner, and had no effect on CD11b expression. Dex, CHX or theophylline dose-dependently decreased CD62L expression on IFN-gamma-stimulated eosinophils.


CONCLUSIONS
IFN-gamma is a particular cytokine which can increase CD62L expression on circulating or infiltrated human eosinophils. It is suggested that protein synthesis and intracellular cAMP participate in the increase in L-selectin expression on IFN-gamma-stimulated eosinophils.
Momose
T
T
First Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Okubo
Y
Y
Horie
S
S
Takashi
S
S
Tsukadaira
A
A
Suzuki
J
J
Isobe
M
M
Sekiguchi
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology; D019041:L-Selectin / Q000096:biosynthesis*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D011994:Recombinant Proteins
D000970:Antineoplastic Agents; D015848:Interleukin-5; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins; D019041:L-Selectin; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor