Integrated approach to the multidimensional analysis of complex biological samples by microseparation techniques. Analysis of glycoprotein factor associated with cancer cachexia.
Microanalytical separation techniques including capillary liquid chromatography, capillary electrophoresis and capillary electrochromatography are suitable for detection of diagnostically important changes in the metabolic profiles of biological fluids. A prototype instrument was employed to serve as an integrated platform for the analysis of urine sample from patients suffering from cancer cachexia. The instrument provides for convenient, rapid and efficient multidimensional approach towards method development which would facilitate simultaneous analysis of complex biological mixtures by the above techniques.
Choudhary
G
G
Biomeasurements Group, Hewlett-Packard Laboratories, Palo Alto, CA 94304, USA.
Hancock
W
W
Witt
K
K
Rozing
G
G
Torres-Duarte
A
A
Wainer
I
I
D002100:Cachexia / Q000209:etiology / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006023:Glycoproteins / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D009369:Neoplasms / Q000150:complications*
D006023:Glycoproteins