title: Relationship between thigmotropism and Candida biofilm formation in vitro.
abstract: The biofilm formation of the oral fungal pathogen Candida on denture acrylic strips coated with saliva or serum was examined in relation to the ability to induce hyphae by thigmotropic reaction, using C. albicans (4 isolates), C. glabrata (3 isolates) and C. tropicalis (3 isolates). Both the degree of biofilm formation and the amount of hyphae exhibiting thigmotropism varied depending upon both the species and strains of Candida. Although there was no significant correlation between the amount of hyphae induced by thigmotropic reaction of fungal isolates and biofilm formation on uncoated control specimens (r = 0.577; p < 0.05), the ability of hyphae induced by thigmotropic reaction significantly correlated with the amount of both saliva- and serum-admixed biofilms (r = 0.734; p < 0.05 and r = 0.793; p < 0.01, respectively). Taken together our in vitro data suggested that the hyphal induction by thigmotropic reaction is of importance in candidal biofilm formation on saliva- or serum-coated acrylic surfaces.
authors.affiliation: Department of Prosthetic Dentistry, Hiroshima University School of Dentistry, Japan. hirocky@ipc.hiroshima-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Nikawa
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Hamada
authors.forename: S
authors.initials: S
authors.lastname: Makihira
authors.forename: L P
authors.initials: LP
authors.lastname: Samaranayake
mesh_terms: D000180:Acrylic Resins; D018441:Biofilms / Q000254:growth & development*; D001769:Blood / Q000382:microbiology; D002175:Candida / Q000472:pathogenicity / Q000502:physiology*; D002176:Candida albicans / Q000472:pathogenicity / Q000502:physiology; D002180:Candidiasis, Oral / Q000209:etiology; D002448:Cell Adhesion; D003764:Dental Materials; D003778:Dentures / Q000009:adverse effects; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D012463:Saliva / Q000382:microbiology; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D003764:Dental Materials