Molecular cloning and expression of the rat monocyte chemotactic protein-3 gene: a possible role in stroke.
Using the suppression subtractive hybridization (SSH) strategy for differential gene cloning, we identified the induced expression of a rat homologue to murine and human monocyte chemotactic protein-3 (MCP-3) in ischemic brain. The 2.4-kilobase rat MCP-3 gene features high homology in gene structure and sequence to murine MCP-3. The temporal expression of MCP-3 mRNA was examined in brain tissue rendered ischemia by permanent or temporary occlusion of the middle cerebral artery (MCAO). A marked increase in MCP-3 mRNA was observed 12 h post-ischemia, with 49-fold and 17-fold increase (n=4, p<0.01) over control in the permanent or temporary MCAO, respectively. Significant induction of MCP-3 in the ischemic cortex was sustained up to 5 days after ischemic injury. The profile of MCP-3 mRNA induction paralleled leukocyte infiltration and accumulation that occur after focal stroke, suggesting a role for MCP-3 in recruiting these inflammatory cells into the ischemic tissue. Molecular cloning of rat MCP-3 should provide a valuable tool, as demonstrated in the present work, for the investigation of MCP-3 expression and function in rat disease models.
Wang
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X
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA. xinkang.wang@dupontpharma.com
Li
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X
Yaish-Ohad
S
S
Sarau
H M
HM
Barone
F C
FC
Feuerstein
G Z
GZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D054410:Chemokine CCL7; D003001:Cloning, Molecular; D016207:Cytokines; D004247:DNA / Q000737:chemistry; D006801:Humans; D020244:Infarction, Middle Cerebral Artery / Q000209:etiology*; D051379:Mice; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment
C515450:CCL7 protein, human; C515451:Ccl7 protein, mouse; C515452:Ccl7 protein, rat; D054410:Chemokine CCL7; D016207:Cytokines; D018945:Monocyte Chemoattractant Proteins; D004247:DNA