[Mutation analysis of p16 gene in non-small cell lung carcinomas].
OBJECTIVE
To investigate the role of p16 gene in the tumorigenesis of non-small cell lung carcinomas (NSCLC).


METHODS
Homozygous deletion and mutation of exon 2 of p16 genes in 40 NSCLC tissues were analyzed using PCR and dsDNA direct sequencing technique.


RESULTS
In 2 NSCLC tissues, homozygous deletions of p16 gene were detected; in 14 NSCLC tissues, 19 point mutations and a frameshift were detected.


CONCLUSIONS
Point mutations of p16 gene were frequent and might play a role in the initiation and progression of NSCLC. Nt380 in this gene was a hot spot of mutation.
Fu
S
S
Department of Medical Genetics, Harbin Medical University, Harbin, Henongjiang, 150086 P. R. China. fusb@ems.hrbmu.edu.cn
Wang
B
B
Huang
C
C
Liu
Q
Q
Zhang
G
G
Li
P
P
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D019942:Genes, p16; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation