Effect of oral rotavirus/iscom vaccines on immune responses in gnotobiotic lambs.
A comparison of the effect on the immune responses in gnotobiotic lambs was made between an iscom vaccine prepared from recombinant rotavirus VP6 protein, an inactivated rotavirus/iscom-matrix vaccine and a vaccine comprising inactivated rotavirus alone. All three vaccines induced immunological priming and some degree of protection was observed after a single oral dose. However, different immune responses were induced in response to a virulent infection. The group vaccinated with the rotavirus/iscom-matrix vaccine showed a Th2-like response characterised by rotavirus-specific antibodies and a down-regulation of IFNgamma in jejunal Peyer's patches. Both Th1-like and Th2-like immune responses were induced in the group receiving the VP6 vaccine as seen by significantly increased expressions of IFNgamma and IL-6 in the jejunal Peyer's patch together with an increased percentage of CD8+ T cells in the intestine and rotavirus-specific antibodies at mucosal surfaces. Iscom vaccines given orally have the ability to induce both Th1-like and Th2-like immune responses in a ruminant model.
van Pinxteren
L A
LA
Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik, Midlothian, UK. lpi@ssi.dk
Bruce
M G
MG
Campbell
I
I
Wood
A
A
Clarke
C J
CJ
Bellman
A
A
Morein
B
B
Snodgrass
D R
DR
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation; D000956:Antigens, Viral; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D005856:Germ-Free Life / Q000276:immunology*; D016882:ISCOMs / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D007583:Jejunum / Q000276:immunology; D010581:Peyer's Patches / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000517:prevention & control; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000008:administration & dosage; D014765:Viral Vaccines / Q000008:administration & dosage*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D016882:ISCOMs; C061332:VP6 protein, Rotavirus; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D007371:Interferon-gamma