Regulation of norepinephrine transporter and tyrosine hydroxylase mRNAs after kainic acid-induced seizures.
Noradrenergic locus coeruleus (LC) efferents to the forebrain suppress seizures in several models of epilepsy. Using in situ hybridization, we demonstrate that tyrosine hydroxylase (TH) and norepinephrine transporter (NET) but not vesicular monoamine transporter 2 (VMAT2) mRNA levels are transiently elevated in LC neurons following kainic acid-induced status epilepticus. These increases of TH and NET mRNAs and presumably of the proteins themselves might enhance synthesis and reuptake of NE postictally.
Bengzon
J
J
Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, S-221 85, Lund, Sweden.
Hansson
S R
SR
Hoffman
B J
BJ
Lindvall
O
O
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D004830:Epilepsy, Tonic-Clonic / Q000139:chemically induced; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology*; D008125:Locus Coeruleus / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D026901:Membrane Transport Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents / Q000096:biosynthesis; D009638:Norepinephrine / Q000378:metabolism*; D050484:Norepinephrine Plasma Membrane Transport Proteins; D016548:Prosencephalon / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism; D027981:Symporters; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis*; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
D002352:Carrier Proteins; D018690:Excitatory Amino Acid Agonists; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D050484:Norepinephrine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; C493454:Slc18a2 protein, rat; C493226:Slc6a2 protein, rat; D027981:Symporters; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins; D014446:Tyrosine 3-Monooxygenase; D007608:Kainic Acid; D009638:Norepinephrine