Detection of the fluorescence of GI vessels in rats using a CCD camera or a near-infrared video endoscope.
BACKGROUND
Choroidal near-infrared fluorescent angiography can detect vessels in the eye with high resolution. Observation of fluorescent gastrointestinal (GI) vessels by endoscopy may be useful in portal hypertension and bleeding ulcer. We here describe a technique for the detection of fluorescent GI vessels with a CCD camera or a near-infrared video endoscope.


METHODS
Laparotomy was performed on rats. A tissue target was excited by means of a laser diode. We took pictures of tissue under both white and near-infrared light, both before and after intravenous injection of indocyanine green. Fluorescent light was selected by means of filters placed in front of the lens of a CCD camera or a near-infrared video endoscope.


RESULTS
Under near-infrared light and after dye injection, we observed fluorescent vessels in real time and distinguished arterial from venous fluorescence.


CONCLUSIONS
This device permits visualization of GI vessels, which could be useful for diagnosis of vascular abnormalities during endoscopy and surgery.
Borotto
E
E
Inserm 451, Loa-Ensta, Ecole Polytechnique, Palaiseau, Le Kremlin-Bicêtre, France.
Englender
J
J
Pourny
J C
JC
Naveau
S
S
Chaput
J C
JC
Lecarpentier
Y
Y
D000818:Animals; D001158:Arteries; D004396:Coloring Agents / Q000528:radiation effects; D004064:Digestive System / Q000098:blood supply*; D020776:Endoscopes, Gastrointestinal; D016099:Endoscopy, Gastrointestinal / Q000379:methods; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D007208:Indocyanine Green / Q000528:radiation effects; D007259:Infrared Rays; D010781:Photography / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014680:Veins; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods
D004396:Coloring Agents; D007208:Indocyanine Green