Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings.
A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the 3'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promoters were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.
Anai
T
T
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kono
N
N
Kosemura
S
S
Yamamura
S
S
Hasegawa
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics*; D018517:Plant Roots / Q000737:chemistry; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000737:chemistry / Q000235:genetics*
D007210:Indoleacetic Acids; D010940:Plant Proteins; C401721:SAUR protein, Raphanus sativus