[Homozygous deletion of CDKN2/p16 gene in lung cancers].
OBJECTIVE
To study the relationship between CDKN2/p16 gene homozygous deletion and lung cancer progression.


METHODS
Improved multiplex PCR technique was applied to detect deletion of CDKN2/p16 gene exon1 and exon2 in 89 cases of lung cancers.


RESULTS
Gene deletion rate was increased by the improved PCR technique. Exon 1 and exon 2 deletion rates were 19.1% (17/89) and 22.5% (20/89) respectively, with total rate of exon1 and/or exon2 deletion 25.8% (23/89). Deletion of CDKN2/p16 gene occurred in non-small cell lung carcinoma (NSCLC) and was related to metastasis and progressive stage.


CONCLUSIONS
Abnormality of CDKN2/p16 gene is a genetic factor for NSCLC susceptibility, and may play a role to some extent in NSCLC malignant progression.
Su
C
C
Molecular Biological Laboratory of Nanjing 81 Hospital, Nanjing, Jiangsu, 210002 P.R.China. sucq@jlonline.com
Ye
Y
Y
Cao
X
X
Shan
X
X
D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction