Evidence of actin in the cytoskeleton of microsporidia.
Using transmission electron microscopy, immuno-electron microscopy, and biochemical techniques such as 2-D electrophoresis and immunoblotting, actin was found in all biological stages of the microsporidia Encephalitozoon hellem and Encephalitozoon cuniculi.
Bigliardi
E
E
Department of Evolutionary Biology, University, Via Mattioli, Sienna, Italy. bigliardi@unisi.it
Riparbelli
M G
MG
Selmi
M G
MG
Bini
L
L
Liberatori
S
S
Pallini
V
V
Bernuzzi
A
A
Gatti
S
S
Scaglia
M
M
Sacchi
L
L
D000199:Actins / Q000032:analysis*; D000818:Animals; D003599:Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D016819:Encephalitozoon / Q000737:chemistry / Q000254:growth & development / Q000648:ultrastructure*; D009670:Encephalitozoon cuniculi / Q000737:chemistry / Q000254:growth & development / Q000648:ultrastructure*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron
D000199:Actins