Induced heteroduplex genotyping of TNF-alpha, IL-1beta, IL-6 and IL-10 polymorphisms associated with transcriptional regulation.
We describe the construction and use of 7 induced heteroduplex generators, reagents for the rapid and unequivocal genotyping of nucleotide sequence polymorphism in TNF-alpha, IL-1beta, IL-6 and IL-10. Polymorphisms detected are those previously associated with regulation of gene transcription: TNF-alpha positions -308 and -238; IL-1beta position +3953; IL-6 position -174; and IL-10 positions -1082, -819 and -592. The reagents were used for analysis of allele and haplotype frequencies in a population of healthy Caucasian volunteer blood donors.
Morse
H R
HR
Division of Transplantation Sciences, University of Bristol, Bristol, UK.
Olomolaiye
O O
OO
Wood
N A
NA
Keen
L J
LJ
Bidwell
J L
JL
D020137:Base Pair Mismatch / Q000235:genetics; D001483:Base Sequence; D005786:Gene Expression Regulation; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D044465:White People / Q000235:genetics
D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D009692:Nucleic Acid Heteroduplexes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10