KIAA0369, doublecortin-like kinase, is expressed during brain development.
During embryonic development, the cerebral cortex attains its characteristic adult laminated structure. The finding that X-linked lissencephaly patients harbor mutations in the doublecortin gene implicated this gene product in the process of corticogenesis. An autosomal human gene, KIAA0369, with a high level of similarity to doublecortin, has been cloned from human adult brain. This gene product contains a kinase domain in addition to a doublecortin-like domain. In order to evaluate whether this doublecortin-like kinase also plays a role during brain development, we cloned and studied the expression pattern of the mouse homolog. Three cDNA products of this gene were cloned: one, doublecortin-like kinase, the second containing only the doublecortin-like region, and the third containing only the kinase domain, a homolog of the previously cloned rat CPG16 gene. We studied doublecortin-like kinase expression in mouse using Northern blot analysis, in situ hybridization, and Western blot analysis, and conclude that doublecortin-like kinase is expressed in multiple regions of embryonic brain including the developing cerebral cortex.
Burgess
H A
HA
Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot, Israel.
Martinez
S
S
Reiner
O
O
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis* / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D000089662:Doublecortin Protein; D000089742:Doublecortin-Like Kinases; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D015347:RNA Probes; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D015347:RNA Probes; C117347:DCLK1 protein, human; D000089742:Doublecortin-Like Kinases; C485525:Dclk1 protein, mouse; C485526:Dclk1 protein, rat; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases