Proliferation of hepatic peroxisomes in rats following the intake of green or black tea.
Rats maintained on green, black or decaffeinated black tea (2.5%, w/v) as their sole drinking fluid displayed higher hepatic CN- insensitive palmitoyl CoA oxidase activity than controls; the extent of increase was similar with the three types of tea. Morphological examination of the liver using electron microscopy revealed an increase in the number of peroxisomes in the tea-treated animals. The same treatment of the animals with green and black tea resulted in a similar rise in hepatic microsomal lauric acid hydroxylation. Analysis by HPLC of the aqueous tea extracts employed in the current study showed that the total flavanol content of the green variety was much higher than the black varieties, and confirmed the absence of caffeine in the decaffeinated black tea. It may be concluded from the present studies that neither caffeine nor flavanoids are likely to be responsible for the proliferation of peroxisomes observed in rats treated with tea.
Bu-Abbas
A
A
School of Biological Sciences, University of Surrey, Guildford, UK.
Dobrota
M
M
Copeland
E
E
Clifford
M N
MN
Walker
R
R
Ioannides
C
C
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007850:Lauric Acids / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D020025:Peroxisome Proliferators / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000009:adverse effects
D007850:Lauric Acids; D020025:Peroxisome Proliferators; D013662:Tea; D010171:Palmitoyl Coenzyme A