Cloning and expression profile of human inorganic pyrophosphatase.
We have cloned a 1.23 kb cDNA from a human heart library which encodes a 32 kDa protein that is 94% identical to bovine inorganic pyrophosphatase. The protein contains an aspartate-rich signature sequence that was previously identified in yeast and prokaryotic pyrophosphatases. Our clone detects a single band on Northern blots and is expressed at modest levels in all tissues examined. The cDNA shows linkage to markers on the long arm of chromosome 10.
Fairchild
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Evanston Northwestern Healthcare Research Institute, 2650 Ridge Avenue, Evanston, IL 60201, USA.
Patejunas
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000235:genetics*
D018076:DNA, Complementary; D011755:Pyrophosphatases