Association of pS2 (TFF1) release with breast tumour proliferative rate: in vitro and in vivo studies.
Although cytosolic expression of the protein pS2 (TFF1) is considered to be a marker of oestrogen receptor (OR) function, there exists some clinical data to suggest an inverse relationship of cytosolic pS2 to tumour proliferation. Although secreted from breast cancer cells, the relationship of pS2 secretion to tumour natural history has been little studied. The mechanisms and kinetics of pS2 release and its relation to tumour cell proliferation were studied in a human breast cancer cell line MCF-7 and verified in a preliminary clinical study. Stimulation by stripped serum or oestradiol resulted in parallel increases of proliferation and pS2 release in both time course and dose-response experiments. Direct pharmacological alterations of proliferation were followed by identical changes in pS2 release. The relationship between serum pS2 levels and tumour proliferative activity when analysed as a function of steroid status showed a slope of 0.56 in OR+ vs. 0.19 in OR- tumours. It is concluded that pS2 release from breast cancer cells is associated with their proliferation and measurement of serum pS2 levels might be a good predictor of tumour proliferative state and could permit noninvasive monitoring of this tumour parameter.
Reshkin
S J
SJ
Department of General and Environmental Physiology, University of Bari, Italy. reshkin@biologia.uniba.it
Tedone
T
T
Correale
M
M
Mangia
A
A
Casavola
V
V
Paradiso
A
A
D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000097:blood / Q000473:pathology* / Q000601:surgery; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free; D003839:Deoxyadenosines / Q000494:pharmacology; D005260:Female; D006133:Growth Substances / Q000097:blood / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D012044:Regression Analysis; D000071163:Trefoil Factor-1; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D000970:Antineoplastic Agents; D016895:Culture Media, Serum-Free; D003839:Deoxyadenosines; D006133:Growth Substances; D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins; D000584:Amiloride; C058120:cordycepin; C039614:ethylisopropylamiloride