Overproduction of L-cysteine and L-cystine by expression of genes for feedback inhibition-insensitive serine acetyltransferase from Arabidopsis thaliana in Escherichia coli.
Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucose. The amount of these amino acids produced per cell was 30 to 60% higher than that of an E. coli strain carrying mutant serine acetyltransferase less sensitive to feedback inhibition.
Takagi
H
H
Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka-cho, Fukui, Japan. hiro@fpu.ac.jp
Awano
N
N
Kobayashi
S
S
Noji
M
M
Saito
K
K
Nakamori
S
S
D000123:Acetyltransferases / Q000037:antagonists & inhibitors*; D017360:Arabidopsis / Q000201:enzymology*; D003545:Cysteine / Q000096:biosynthesis*; D003553:Cystine / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005246:Feedback; D051050:Serine O-Acetyltransferase
D003553:Cystine; D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D003545:Cysteine