Antibodies against pex14p block ATP-independent binding of matrix proteins to peroxisomes in vitro.
The membrane protein Pex14p is a key component of the protein import machinery of peroxisomes. Antibodies raised against human Pex14p recognise a 66 kDa protein in sunflower glyoxysomes (HaPex14p) and immunoprecipitate in vitro-translated Arabidopsis Pex14p (AtPex14p). These antibodies inhibit the ATP-independent binding to sunflower peroxisome membranes of peroxisome targeting signal type (PTS) 1- and PTS2-targeted matrix proteins, but not an integral membrane protein. These results suggest that Pex14p functions before the ATP-dependent step of peroxisome assembly.
Lopez-Huertas
E
E
Centre for Plant Sciences, Leeds Institute of Plant Biotechnology and Agriculture, University of Leeds, Leeds, UK.
Oh
J
J
Baker
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology*; D001692:Biological Transport; D002254:Carbonates / Q000378:metabolism; D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000276:immunology* / Q000378:metabolism; D020676:Glyoxysomes / Q000276:immunology / Q000378:metabolism; D006368:Helianthus; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D008970:Molecular Weight; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000276:immunology / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012097:Repressor Proteins
D000906:Antibodies; D002254:Carbonates; D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; C455539:PEX14 protein, human; C489785:PXMP2 protein, human; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D012097:Repressor Proteins; D000255:Adenosine Triphosphate