Analysis of Salmonella enterica serotype Typhimurium by phage typing, antimicrobial susceptibility and pulsed-field gel electrophoresis.
Three typing methods commonly used for bacteria--phage typing, antimicrobial susceptibility and pulsed-field gel electrophoresis (PFGE)- were used to characterise 64 Salmonella enterica serotype Typhimurium isolates from individual adult patients from Nairobi, Kenya. The isolates encompassed 11 definitive phage types (DTs), which fell into eight PFGE clusters; 31.3% of isolates were either untypable or reacted nonspecifically with the phages used for typing and 26.6% were of DT 56. Plasmids of c. 100 kb were responsible for self-transferable multiresistance among the isolates. Analysis by PFGE and phage type demonstrated that multiresistant Typhimurium strains causing diarrhoea and invasive disease were multiclonal.
Kariuki
S
S
Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 54840, Nairobi, Kenya.|School of Tropical Medicine, University of Liverpool, Liverpool, L69 3GA.|Department of Medical Microbiology and Genitourinary Medicine.
Gilks
C
C
School of Tropical Medicine, University of Liverpool, Liverpool, L69 3GA.
Kimari
J
J
Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 54840, Nairobi, Kenya.
Muyodi
J
J
Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 54840, Nairobi, Kenya.
Waiyaki
P
P
Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 54840, Nairobi, Kenya.
Hart
C A
CA
Department of Medical Microbiology and Genitourinary Medicine.
D000328:Adult; D001434:Bacteriophage Typing; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D011815:R Factors; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification* / Q000187:drug effects / Q000235:genetics
D004269:DNA, Bacterial