title: Technical manual for manufacturing autologous fibrin tissue adhesive.
abstract: OBJECTIVE
  The aim of this article is to provide a concise and simple technical manual for manufacturing autologous fibrin tissue adhesive derived from the precipitation of fibrinogen using a combination of ethanol and freezing for surgery.
  
  
  METHODS
  All materials and equipment needed to manufacture ethanol-based autologous fibrin tissue adhesive are listed. In addition, step-by-step instructions are provided to allow for easy and rapid fibrin adhesive production.
  
  
  RESULTS
  Ethanol-based autologous fibrin tissue adhesive can be manufactured in under 60 minutes. Furthermore, at our institution the startup cost for manufacturing ethanol-based autologous fibrin tissue adhesive was under $2,500.00.
  
  
  CONCLUSIONS
  Ethanol-based autologous fibrin tissue adhesive is a safe, reliable, and easily manufactured autologous fibrin tissue adhesive that can be made by a trained technician in any blood bank, pharmacy, or surgical laboratory.
authors.affiliation: Department of Surgery, The University of Illinois Hospital and Clinics, Chicago, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Park
authors.forename: J R
authors.initials: JR
authors.lastname: Cintron
authors.forename: K H
authors.initials: KH
authors.lastname: Siedentop
authors.forename: C P
authors.initials: CP
authors.lastname: Orsay
authors.forename: R K
authors.initials: RK
authors.lastname: Pearl
authors.forename: R L
authors.initials: RL
authors.lastname: Nelson
authors.forename: H
authors.initials: H
authors.lastname: Abcarian
mesh_terms: D000431:Ethanol; D015718:Fibrin Tissue Adhesive / Q000138:chemical synthesis; D005340:Fibrinogen; D005615:Freezing; D006801:Humans; D014014:Tissue Adhesives / Q000138:chemical synthesis
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D000431:Ethanol; D005340:Fibrinogen