Syntheses and immunologic properties of Escherichia coli O157 O-specific polysaccharide and Shiga toxin 1 B subunit conjugates in mice.
Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. Mice injected with these bivalent conjugates elicited both bactericidal antibodies to E. coli O157 and neutralization antibodies to Stx1.
Konadu
E
E
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2720, USA.
Donohue-Rolfe
A
A
Calderwood
S B
SB
Pozsgay
V
V
Shiloach
J
J
Robbins
J B
JB
Szu
S C
SC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D019453:Escherichia coli O157 / Q000276:immunology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D019081:O Antigens / Q000276:immunology*; D022603:Shiga Toxins; D018074:Vaccines, Conjugate / Q000276:immunology
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D019081:O Antigens; D022603:Shiga Toxins; D018074:Vaccines, Conjugate