[Internalization and replication of Mycobacterium tuberculosis and M. avium complex within type II alveolar epithelial cell line].
Profiles of internalization and replication of Mycobacterium tuberculosis (MTB) or M. avium complex (MAC) within A-549 human type II alveolar epithelial cell line (A-549 cells) were studied and the results were compared with the mode of internalizing and proliferative behaviors of the organisms within murine peritoneal macrophages (M phi s) and various M phi-like cell lines (murine J774A. 1, human THP-1, and human MONO-MAC-6). First, MTB and MAC internalized not only in peritoneal M phi s and M phi-like cell lines but also in A-549 cells. Secondly, MTB and MAC replicated within A-549 cells and these organisms displayed much more vigorous intracellular multiplication in A-549 cells than in murine peritoneal M phi s and J774A. 1 M phi-like cell line. Human M phi-like cell lines (THP-1, MONO-MAC-6) allowed the growth of MTB and MAC equally or occasionally more rapidly, as compared to the case of A-549 cells.
Sato
K
K
Department of Microbiology and Immunology, Shimane Medical University, Japan.
Ogasawara
K
K
Akaki
T
T
Tomioka
H
H
D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D015269:Mycobacterium avium Complex / Q000254:growth & development*; D009169:Mycobacterium tuberculosis / Q000254:growth & development*; D011650:Pulmonary Alveoli / Q000382:microbiology*