title: The complete nucleotide sequence of RNA2 of blackcurrant reversion nepovirus.
abstract: The complete nucleotide sequence of blackcurrant reversion nepovirus (BRV) RNA2 was determined from cDNA clones. RNA2 was 6400 nucleotides (nt) in length excluding the 3' poly(A)-tail. It contained a single open reading frame of 4878 nts encoding a polypeptide of 1626 amino acids with a calculated M(r) of 178¿ omitted¿860. The genome organization of BRV RNA2 was similar to that of other nepoviruses, especially those with a large RNA2. The coat protein (CP) was located in the C-terminal region of the large polyprotein and contained amino acid motifs conserved among nepovirus CPs. Sequence comparisons revealed a proline (P) residue surrounded by hydrophobic amino acid residues located upstream of the CP. This P motif is conserved among the putative movement proteins of nepo-, como-, caulimo- and capilloviruses. An N-terminal domain of 350 amino acids of RNA2-encoded polyprotein shared 34 and 35% sequence identity with the N-terminal domains of tomato ringspot nepovirus RNA1- and RNA2-encoded polyproteins, respectively. Sequence identities between the N-terminal domains of BRV RNA2 and other nepoviral RNA2s were less than 20%; no common N-terminal motif was found.
authors.affiliation: Laboratory of Plant Physiology and Molecular Biology, University of Turku, Turku, FIN-20014, Finland. satu.latvala @utu.fi
authors.forename: S
authors.initials: S
authors.lastname: Latvala-Kilby
authors.forename: K
authors.initials: K
authors.lastname: Lehto
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral; D019684:Magnoliopsida / Q000821:virology*; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral