title: Distribution of the cfiA gene among Bacteroides fragilis strains in Japan and relatedness of cfiA to imipenem resistance.
abstract: The cfiA gene, encoding an imipenem-hydrolyzing metallo-beta-lactamase produced by Bacteroides fragilis, and insertion-like elements were detected by PCR amplification with B. fragilis strains isolated in Japan. The cfiA gene was found in 1.9 and 4.1% of the imipenem-susceptible B. fragilis isolates collected from 1987 to 1988 and from 1992 to 1994, respectively. Insertion-like elements adjacent to the cfiA gene were found in all nine metallo-beta-lactamase-producing imipenem-resistant strains tested but not in nine cfiA-positive strains with no detectable metallo-beta-lactamase activity.
authors.affiliation: Institute of Anaerobic Bacteriology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamazoe
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Katagiri
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D001426:Bacterial Proteins; D001441:Bacteroides fragilis / Q000187:drug effects* / Q000235:genetics*; D004352:Drug Resistance, Microbial; D015378:Imipenem / Q000494:pharmacology*; D015345:Oligonucleotide Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase