Separation of ovomucin subunits by gel filtration: enhanced resolution of subunits by using a dual-column system.
An improved procedure involving a dual-column gel-filtration system for the separation of ovomucin subunits is described. Ovomucin, prepared from thick egg albumin by precipitation, was separated in the presence of sodium dodecyl sulfate (SDS) and beta-mercaptoethanol by using two Sephacryl S-400 HR gel-filtration columns connected in series. The separation resulted in the isolation of eight peaks. The amino acid compositions and SDS-polyacrylamide gradient gel electrophoretic pattern of each peak were determined.
Hiidenhovi
J
J
Agricultural Research Centre of Finland, Food Research, Food Chemistry and Technology, FIN-31600 Jokioinen, Finland. jaakko.hiidenhovi@mtt.fi
Aro
H S
HS
Kankare
V
V
D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel / Q000379:methods; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D010047:Ovalbumin / Q000302:isolation & purification; D010059:Ovomucin / Q000737:chemistry* / Q000302:isolation & purification
D000596:Amino Acids; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D010059:Ovomucin; D010047:Ovalbumin