Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent.
The induction of T-cell apoptosis is regulated in part by monocytes (CD14+ cells). Human peripheral blood monocytes inhibited the spontaneous cell death of activated T cells in vitro. The inhibition of T-cell apoptosis did not require autologous monocytes. Inhibition required direct contact with monocytes and was not due to a soluble factor. Furthermore, treatment of monocytes with actinomycin D, cycloheximide and paraformaldehyde abrogated the anti-apoptotic activity of these cells. Blocking antibody to CD40 and CD154 (CD40 ligand) decreased the ability of monocytes to aid in T-cell survival, whereas, blocking LFA-1/I-CAM-1, Fas ligand and the CD4/major histocompatibility complex class II interaction did not affect the influence of monocytes on T-cell survival. This shows that monocytes rescue of activated T cells from apoptosis is dependent upon CD40/CD154 interaction.
Sakata
K
K
University of Texas Health Science Center, San Antonio, TX 78284, USA.
Sakata
A
A
Kong
L
L
Vela-Roch
N
N
Talal
N
N
Dang
H
H
D019138:Antibodies, Blocking / Q000494:pharmacology; D017209:Apoptosis / Q000276:immunology*; D019013:CD40 Antigens / Q000378:metabolism*; D023201:CD40 Ligand; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D011108:Polymers / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*
D019138:Antibodies, Blocking; D019013:CD40 Antigens; D008024:Ligands; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D011108:Polymers; D023201:CD40 Ligand; D003609:Dactinomycin; D005557:Formaldehyde; D003513:Cycloheximide; C003043:paraform