title: Pyrococcus glycovorans sp. nov., a hyperthermophilic archaeon isolated from the East Pacific Rise.
abstract: A hyperthermophilic archaeon, strain AL585T, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at latitude 13 degrees N and a depth of 2650 m. The isolate was a strictly anaerobic coccus with a mean cell diameter of 1 micron. The optimum temperature, pH and concentration of sea salt for growth were 95 degrees C, 7.5 and 30 g l-1. Under these conditions, the doubling time and cell yield were 0.5 h and 5 x 10(8) cells ml-1. Strain AL585T grew preferentially in media containing complex proteinaceous carbon sources, glucose and elemental sulfur. The G + C content of the DNA was 47 mol%. Sequencing of the 16S rDNA gene showed that strain AL585T belonged to the genus Pyrococcus and was probably a new species. This was confirmed by total DNA hybridization. Consequently, this strain is described as a new species, Pyrococcus glycovorans sp. nov.
authors.affiliation: Laboratoire de Caractérisation des Microorganismes Marins, IFREMER, Centre de Brest, Plouzané, France. gbarbier@ifremer.fr
authors.forename: G
authors.initials: G
authors.lastname: Barbier
authors.forename: A
authors.initials: A
authors.lastname: Godfroy
authors.forename: J R
authors.initials: JR
authors.lastname: Meunier
authors.forename: J
authors.initials: J
authors.lastname: Quérellou
authors.forename: M A
authors.initials: MA
authors.lastname: Cambon
authors.forename: F
authors.initials: F
authors.lastname: Lesongeur
authors.forename: P A
authors.initials: PA
authors.lastname: Grimont
authors.forename: G
authors.initials: G
authors.lastname: Raguénès
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D019711:Pyrococcus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S