The absence of interleukin 1 receptor-related T1/ST2 does not affect T helper cell type 2 development and its effector function.
T1/ST2, an orphan receptor with homology with the interleukin (IL)-1 receptor family, is expressed constitutively and stably on the surface of T helper type 2 (Th2) cells, but not on Th1 cells. T1/ST2 is also expressed on mast cells, which are critical for Th2-mediated effector responses. To evaluate whether T1/ST2 is required for Th2 responses and mast cell function, we have generated T1/ST2-deficient (T1/ST2(-/-)) mice and examined the roles of T1/ST2. Naive CD4(+) T cells isolated from T1/ST2(-/-) mice developed to Th2 cells in response to IL-4 in vitro. T1/ST2(-/-) mice showed normal Th2 responses after infection with the helminthic parasite Nippostrongylus brasiliensis as well as in the mouse model of allergen-induced airway inflammation. In addition, differentiation and function of bone marrow-derived cultured mast cells were unaffected. These findings demonstrate that T1/ST2 does not play an essential role in development and function of Th2 cells and mast cells.
Hoshino
K
K
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Kashiwamura
S
S
Kuribayashi
K
K
Kodama
T
T
Tsujimura
T
T
Nakanishi
K
K
Matsuyama
T
T
Takeda
K
K
Akira
S
S
D000818:Animals; D002478:Cells, Cultured; D007249:Inflammation / Q000209:etiology; D007371:Interferon-gamma / Q000502:physiology; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008407:Mast Cells / Q000502:physiology; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009559:Nippostrongylus / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D011506:Proteins / Q000502:physiology*; D018123:Receptors, Interleukin; D017472:Receptors, Interleukin-1 / Q000502:physiology*; D017206:Strongylida Infections / Q000276:immunology; D018418:Th2 Cells / Q000502:physiology*
C486189:Il1rl1 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D011506:Proteins; D018123:Receptors, Interleukin; D017472:Receptors, Interleukin-1; D007371:Interferon-gamma; D010047:Ovalbumin