title: Migration of CD18-deficient neutrophils in vitro: evidence for a CD18-independent pathway induced by IL-8.
abstract: Neutrophils isolated from a child with severe leukocyte adhesion deficiency 1 (LAD1) had a complete absence of expression of the CD11/CD18 beta2 integrin family of adhesion molecules, and were shown to be deficient in the in vitro adhesion and migration properties. However, we found that interleukin-8 (IL8), a potent chemoattractant for neutrophils, and sputum sol phase induced these LAD1 neutrophils to migrate through an endothelial cell layer in vitro, and confirmed that this migration was CD18-independent. These findings add to evidence of CD18-independent mechanisms of neutrophil recruitment, in particular neutrophil infiltration into the lungs, where IL8 may be an important recruitment factor.
authors.affiliation: Department of Medicine, Queen Elizabeth Hospital, Edgbaston, Birmingham, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Morland
authors.forename: B J
authors.initials: BJ
authors.lastname: Morland
authors.forename: P J
authors.initials: PJ
authors.lastname: Darbyshire
authors.forename: R A
authors.initials: RA
authors.lastname: Stockley
mesh_terms: D000906:Antibodies / Q000378:metabolism; D018845:CD11 Antigens / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology*; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D015194:Diffusion Chambers, Culture; D004730:Endothelium, Vascular / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D016167:Integrin alphaXbeta2; D016209:Interleukin-8 / Q000494:pharmacology*; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000276:immunology* / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D011233:Precipitin Tests
chemical_list: D000906:Antibodies; D018845:CD11 Antigens; D018821:CD18 Antigens; D016167:Integrin alphaXbeta2; D016209:Interleukin-8; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins