Ly-49CB6 NK inhibitory receptor recognizes peptide-receptive H-2Kb.
NK-mediated cytotoxicity involves two families of receptors: activating receptors that trigger lysis of the target cells being recognized and inhibitory receptors specific primarily for MHC I on the target cell surface that can override the activating signal. MHC I molecules on the cell surface can be classified into molecules made stable by the binding of peptide with high affinity or unstable molecules potentially capable of binding high affinity peptide (hence, peptide receptive) and being converted into stable molecules. It has been previously shown that the Ly-49A inhibitory receptor recognizes stable Dd molecules. We show in this study that the inhibitory receptor Ly-49CB6 recognizes peptide-receptive Kb molecules, but does not recognize Kb molecules once they have bound high affinity peptide.
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Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Canada.
Kung
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Silver
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Lemieux
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Kane
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Miller
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D000818:Animals; D000918:Antibody Specificity; D000950:Antigens, Ly; D001666:Binding Sites, Antibody; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003433:Crosses, Genetic; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000096:biosynthesis / Q000378:metabolism*; D007113:Immunity, Innate; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D008822:Mice, Transgenic; D010455:Peptides / Q000276:immunology* / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D055630:Receptors, NK Cell Lectin-Like
D000950:Antigens, Ly; D002352:Carrier Proteins; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; D011971:Receptors, Immunologic; D055630:Receptors, NK Cell Lectin-Like; D003208:Concanavalin A