Inhibition of DNA synthesis in isolated human endometrial cells by a levonorgestrel-releasing intrauterine device.
OBJECTIVE
To estimate the effect of an intrauterine device (IUD) releasing 20 micrograms levonorgestrel (LNG) per 24 hours on DNA synthesis in human endometrial cells before and after 12 months of use.


METHODS
Endometrial specimens were collected from the anterior or posterior wall of the miduterus from 6 females on cycle day 10-12 before insertion of the IUD and after 12 months of use.


RESULTS
Previous results from our group did not reveal any influence on endometrial DNA cell content when a levonorgestrel IUD releasing 2 micrograms/24 h was used for 12 months in a group of fertile females. In this study, the IUD release rate, 20 micrograms LNG/24 h, was statistically significantly different from the results in the previous studies. The effect of the levonorgestrel IUD on endometrial proliferation was dose dependent, and a significant correlation could be found between continuous exposure to LNG and inhibition of DNA synthesis in endometrial cells.


CONCLUSIONS
Inhibition of proliferative activity in endometrial cells seems to be reflected by a decrease in DNA synthesis per cell nucleus and contributes to the clinical performance of the LNG-releasing IUD.
Luo
H
H
National Research Institute for Family Planning, Beijing, China.
Zhu
P
P
Xu
R
R
Cheng
J
J
Zhao
X
X
Wang
J D
JD
D000328:Adult; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D003271:Contraceptive Agents, Female / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D004171:Diploidy; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D019044:Image Cytometry; D007436:Intrauterine Devices, Medicated; D016912:Levonorgestrel / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects
D003271:Contraceptive Agents, Female; D016912:Levonorgestrel; D004247:DNA