An integrated decoupler for capillary electrophoresis with electrochemical detection: application to analysis of brain microdialysate.
An approach to capillary electrophoresis with electrochemical detection (CE-EC) suitable for determination of dopamine in 1-min brain microdialysate samples is described. The CE-EC system includes an electrochemical detection cell that permits easy, precise, and permanent alignment of a carbon fiber microelectrode with a separation capillary (30-micron i.d., 75-cm length). Amperometric detection was performed at a constant applied potential of 600 mV with respect to a Ag/AgCl reference electrode. Decoupling of the electrophoretic current from the amperometric detector was accomplished with an integrated end-column decoupler prepared by etching the capillary outlet with HF. The decoupler produces baseline noise of 50 fA, or less, in the presence of 10-20-muA current in the separation capillary. The low baseline noise affords low mass (attomoles) and low concentration (nanomolar) detection limits for dopamine and 4-methylcatechol. A peak attributable to dopamine was identified in electropherograms of brain microdialysate samples obtained from anesthetized rats. Identification of the dopamine peak was confirmed by pharmacological methods. Dopamine was readily detected in 1-min brain microdialysate samples. The dopamine concentration in 1-min brain microdialysis samples was significantly altered by drug treatments and by brief electrical stimulation of dopaminergic axons.
Qian
J
J
Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA.
Wu
Y
Y
Yang
H
H
Michael
A C
AC
D000818:Animals; D001923:Brain Chemistry; D004298:Dopamine / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D017551:Microdialysis / Q000295:instrumentation*; D051381:Rats
D004298:Dopamine