title: Study of immunohistochemical expression of insulin-like growth factor I and proliferating cell nuclear antigen in thyroid gland papillary carcinoma and its metastasis.
abstract: BACKGROUND
  Several tumor factors are associated with papillary thyroid cancer. Most studies do not compare the expressions of these factors in the primary tumors and in their associated cervical metastasis.
  
  
  METHODS
  Paraffin sections of 20 patients with papillary carcinoma of the thyroid gland with lymph node metastasis were studied. The presence and distribution of insulin-like growth factor I (IGF-I) and proliferating cell nuclear antigen (PCNA) was analyzed, through immunohistochemical technique, in both primaries and lymph node metastasis. The results were correlated with clinical-pathologic data (sex, age, size of primary, multicentricity, thyroid capsule invasion, lymphatic and blood vessels invasion, development of distant metastasis, and associated thyroid diseases).
  
  
  RESULTS
  The qualitative analysis showed the reaction for IGF-I was present in more than 90% of the neoplastic cells in both primaries and lymph node metastasis. No correlation with the clinical-pathological features was observed. Regarding the PCNA, the mean percentage of nuclei stained showed no statistical difference between primaries and metastasis (p = 0.598). Except for age, clinicopathologic data had no influence on the mean percentage of nuclei stained. A correlation was verified between the percentage of cells stained by PCNA in primary tumors and the patients' age (p < 0. 01).
  
  
  CONCLUSIONS
  The expressions of these tumor factors are equally intense for both primary and metastatic tissue in papillary thyroid cancer. Despite the small size of the sample, the expressions of IGF-I and PCNA could not be associated to clinical-pathologic features, except for the age. As patients over 40 years old had higher expression of PCNA, this marker may have prognostic significance for patients with papillary thyroid cancer.
authors.affiliation: Department of Surgery, University of São Paulo, São Paulo, Brazil.
authors.forename: G B
authors.initials: GB
authors.lastname: Silva Filho
authors.forename: R M
authors.initials: RM
authors.lastname: Maciel
authors.forename: M H
authors.initials: MH
authors.lastname: Takahashi
authors.forename: V N
authors.initials: VN
authors.lastname: Alberti
authors.forename: I V
authors.initials: IV
authors.lastname: Castro
authors.forename: P H
authors.initials: PH
authors.lastname: Saldiva
authors.forename: M D
authors.initials: MD
authors.lastname: Durazzo
authors.forename: A R
authors.initials: AR
authors.lastname: Ferraz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000737:chemistry* / Q000556:secondary; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D013964:Thyroid Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D007334:Insulin-Like Growth Factor I