[Biochemical changes in apoptosis and methods for their determination (review)].
Apoptosis or programmed cell death is a physiological process which occurs at different biological states as well as at disease process. Morphologically it is characterized by the chromatine condensation and other changes with preserved integrity of plasmatic membrane. The major and most frequently studied biochemical characteristic of apoptosis is a DNA fragmentation. In our paper attention is directed to the early biochemical changes in cell membranes, i.g., the externalization of phosphatidylserine, hydrolysis of sphingomyeline on the ceramide and activation of phospholipases especially phospholipase A2. In one part we described the changes of cysteine proteases (caspases), which play a key role in the execution of apoptosis. These biochemical changes are associated with ceramide signalization of apoptosis. Briefly are presented also some dates about apoptosis induction with reactive oxygen radicals and the role of the arachidonic acid metabolites in this process. We consider the investigation and determination of these changes as important parameters of apoptosis at some diseases, e.g., cancer or degenerative diseases, and of their treatment.
Sedláková
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Ustav farmakológie Lekárskej fakulty Univerzity P. J. Safárika, Kosice.
Kohút
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Kalina
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D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism
D010718:Phosphatidylserines; D013109:Sphingomyelins; D010740:Phospholipases; D020169:Caspases