title: Isolation and Characterization of a Type 1 Ribosome-Inactivating Protein from Fruiting Bodies of the Edible Mushroom (Volvariella volvacea).
abstract: A novel single-chained ribosome-inactivating protein (RIP) with a molecular weight of approximately 29 000 was purified from fruiting bodies of the edible mushroom Volvariellavolvacea with a procedure involving ammonium sulfate precipitation, ion-exchange chromatography on DEAE-cellulose, and gel filtration on Superdex 75. The mushroom RIP, designated volvarin, exhibited a potent inhibitory action on protein synthesis in the rabbit reticulocyte lysate system with an IC(50) value of 0.5 nM. Like most plant RIPs, volvarin acted as an N-glycosidase that depurinated rRNA from rabbit reticulocyte lysate, releasing a characteristic RNA fragment after treatment with aniline. It also exerted a deoxyribonuclease activity on supercoiled SV-40 DNA and demonstrated a strong abortifacient effect in mice.
authors.affiliation: Department of Biology, Graduate School, Academia Sinica, Beijing, China, and Departments of Biology and Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors.initials: QZ
authors.lastname: Yao
authors.initials: MM
authors.lastname: Yu
authors.initials: LS
authors.lastname: Ooi
authors.initials: TB
authors.lastname: Ng
authors.initials: ST
authors.lastname: Chang
authors.initials: SS
authors.lastname: Sun
authors.initials: VE
authors.lastname: Ooi