title: Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution.
abstract: Analytical and preparative high-speed counter-current chromatography was successfully used for the isolation and purification of hydroxyanthraquinones from Rheum officinale Baill (Dahuang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion and aloe-emodin were isolated each at over 98% purity.
authors.affiliation: Beijing Institute of New Technology Application, China.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Tian
authors.forename: H
authors.initials: H
authors.lastname: Cao
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000880:Anthraquinones / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal; D012250:Rheum / Q000737:chemistry*
chemical_list: D000880:Anthraquinones