Characterization of Bacillus thuringiensis ser. balearica (Serotype H48) and ser. navarrensis (serotype H50): two novel serovars isolated in Spain.
The novel strains of Bacillus thuringiensis PM9 and NA69, isolated from soil samples in Spain, were classified and characterized in terms of their crystal proteins, plasmid profile, cry genes content, and their toxicological properties against several species of Lepidoptera, Coleoptera, and Diptera. Both strains share morphological and biochemical characteristics with previously described B. thuringiensis strains, although their unique H antigens identify them as two new serotypes. Two new serovar names, B. thuringiensis serovar balearica (H serotype 48) and B. thuringiensis serovar navarrensis (H serotype 50) are proposed for the type strains PM9 and NA69, respectively.
Iriarte
J
J
Laboratorio de Entomología Agrícola y Patología de Insectos, Departamento de Producción Agraria, Universidad Pública de Navarra, Campus Arrosadía, 31006 Pamplona, Spain.
Dumanoir
V C
VC
Bel
Y
Y
Porcar
M
M
Ferrandis
M D
MD
Lecadet
M
M
Ferré
J
J
Caballero
P
P
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001427:Bacterial Toxins; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006460:Hemolysin Proteins; D015151:Immunoblotting; D002479:Inclusion Bodies; D007313:Insecta / Q000502:physiology*; D010572:Pest Control, Biological; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012703:Serotyping; D012988:Soil Microbiology
D000942:Antigens, Bacterial; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; C022863:H antigen; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis