In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces.
Hydroxylated and methylated surfaces were prepared by the self-assembled monolayer technique (SAM) of alkane thiols on gold. The surfaces were used to evaluate the influence of implant surface chemistry on protein deposition and inflammatory cell response. Implants were inserted subcutaneously in the rat for 3 and 24 h. The surface chemical properties influenced the in vitro rat plasma protein adsorption (ellipsometry/antibody) with few exceptions (albumin not found and fibrinogen always found). The number of recruited cells and their distribution (DNA from implant versus from exudate) was influenced by the different chemistries at 24 h, but not at 3 h. HIS48+, ED1+, ED2+ and small numbers of CD5+ cells were present in the exudate at both time periods (flow cytometry). The cellular oxidative metabolism was low, although cells on -OH surfaces responded with the highest phorbol ester-stimulated chemiluminescence (CL)/DNA. The levels of cytokines IL-1alpha, IL-1beta and TNFalpha (ELISA) were not influenced by material surface chemistry. Sham operated sites had a higher cytokine concentration/DNA compared with exudates from an implant milieu. The results of this study show that surface chemical functionalization modifies specific events in the inflammatory response around implants in soft tissues.
Källtorp
M
M
Institute of Anatomy and Cell Biology, Biomaterials Research Group, Göteborg University, Sweden. kalltorp@anatcell.gu.se
Oblogina
S
S
Jacobsson
S
S
Karlsson
A
A
Tengvall
P
P
Thomsen
P
P
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000378:metabolism; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005122:Exudates and Transudates / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry; D006900:Hydroxylation; D007375:Interleukin-1 / Q000737:chemistry; D007378:Interleukins / Q000378:metabolism; D008163:Luminescent Measurements; D008422:Materials Testing; D008745:Methylation; D020556:Neutrophil Infiltration / Q000276:immunology*; D010084:Oxidation-Reduction; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000378:metabolism
D001672:Biocompatible Materials; D001798:Blood Proteins; D016207:Cytokines; D007375:Interleukin-1; D007378:Interleukins; D013438:Sulfhydryl Compounds; D014409:Tumor Necrosis Factor-alpha; D006046:Gold