Different morphological changes of two colorectal adenocarcinoma cell lines after sodium butyrate treatment.
The principal aim of this study was to determine whether the sodium butyrate, cell differentiation-inducing compound, induces identical morphological changes in two colorectal adenocarcinoma cell lines which exhibit the different changes in the alkaline phosphatase activity after treatment with this agent. Ultrastructural analysis showed that these two cell lines possessed different sensitivity to the presence of sodium butyrate. Particularly different changes were observed in the chromatin structure of the cell lines tested. Our study demonstrates that sodium butyrate initiates cell differentiation, modifies the cell components, but the characteristics and extent of this modification depends on the cell line used.
Stokrová
J
J
Department of Biopolymer Micromorphology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 166 37 Prague 6, Czech Republic.
Sovová
V
V
Sloncová
E
E
Korb
J
J
D000107:Acetylation / Q000187:drug effects; D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology*; D000469:Alkaline Phosphatase / Q000032:analysis; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000473:pathology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002087:Butyrates; D002843:Chromatin; D006657:Histones; D009363:Neoplasm Proteins; D000469:Alkaline Phosphatase