[Reference ranges of antioxidant parameters in whole blood (erythrocytes) in a Thüringen region].
BACKGROUND
The oxidant stress is characterized by measurement of the activities of glutathione peroxidase, superoxiddismutase and also by concentrations of glutathione and selenium in erythrocytes. A standardization of the methods of determination is very important.


METHODS
In erythrocytes of blood donors (n = 101) the parameters glutathione peroxidase, glutathione, superoxiddismutase and selenium were determined.


RESULTS
The following results of the antioxidant parameters in erythrocytes of blood donors were found: Selenium 67.1 +/- 20.1 nmol/mmol Hb, glutathione peroxidase 842 +/- 290 U/mmol Hb, glutathione 108 +/- 48 mumol/mmol Hb, superoxiddismutase 15.8 +/- 6.4 U/mumol Hb.


CONCLUSIONS
Selenium, glutathione peroxidase, glutathione and superoxiddismutase in erythrocytes of blood donors are normally distributed. There are no significant differences between men and women. The use of "own reference values" is necessary because no standardization of the methods of determination exists.
Winnefeld
K
K
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Friedrich-Schiller-Universität Jena. winnefeld@bach.med.uni-jena.de
Streck
S
S
Treff
E
E
Jütte
H
H
Kroll
E
E
Marx
H
H
D000328:Adult; D001782:Blood Donors; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005858:Germany; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D008297:Male; D012016:Reference Values; D012643:Selenium / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood*
D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium