title: Frequent detection of HIV-1 in the gastric aspirates of neonates born to HIV-infected mothers.
abstract: OBJECTIVE
  To evaluate the frequency and correlates of oral route exposure of infants born to HIV-1-infected women.
  
  
  METHODS
  A multicenter study was performed within the prospective French Perinatal Cohort Study of mother-to-child HIV transmission. Oropharyngeal and gastric aspirates from 122 neonates were studied by reverse transcriptase (RT) polymerase chain reaction (PCR) for the presence of HIV-1, as well as for standard microbiology (Gram staining and culture).
  
  
  RESULTS
  Aspirates from 101 neonates were analyzed by RT-PCR; 28% of these were positive for HIV RNA. Another 21 aspirates could not be tested because of PCR inhibition. The median concentration of HIV RNA in the positive aspirates was 126 copies/ml (range: 8-1270). Detection of HIV-1 in the aspirate was significantly related to high maternal plasma-viral load, presence of blood in the aspirate, positive Gram stain or culture, episiotomy or perineal lesions, and sexually transmitted infections during the pregnancy. Most of the mothers received zidovudine prophylaxis during pregnancy and delivery. Among the six infants who were infected with HIV, three had positive aspirates. Of the three assumed to have acquired the infection intrapartum, only one had an HIV RNA-positive aspirate.
  
  
  CONCLUSIONS
  Exposure of the fetus to HIV via the oral route occurs frequently, even in the presence of zidovudine prophylaxis, and is likely to be one of the mechanisms of intrapartum transmission, but not the only one.
authors.affiliation: Service de Gynécologie-Obstétrique I, Centre Hospitalier Universitaire Cochin-Port Royal, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Mandelbrot
authors.forename: M
authors.initials: M
authors.lastname: Burgard
authors.forename: J P
authors.initials: JP
authors.lastname: Teglas
authors.forename: J L
authors.initials: JL
authors.lastname: Bénifla
authors.forename: C
authors.initials: C
authors.lastname: Khan
authors.forename: P
authors.initials: P
authors.lastname: Blot
authors.forename: E
authors.initials: E
authors.lastname: Vilmer
authors.forename: S
authors.initials: S
authors.lastname: Matheron
authors.forename: G
authors.initials: G
authors.lastname: Firtion
authors.forename: S
authors.initials: S
authors.lastname: Blanche
authors.forename: M J
authors.initials: MJ
authors.lastname: Mayaux
authors.forename: C
authors.initials: C
authors.lastname: Rouzioux
mesh_terms: D000328:Adult; D005260:Female; D005750:Gastric Juice / Q000821:virology*; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D009960:Oropharynx / Q000821:virology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000503:physiopathology* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral