Genomic organization and genetic mapping of the bovine PREF-1 gene.
As a potential regulator of nutrient partitioning in beef cattle, we have cloned and genetically mapped the bovine PREF-1 gene. A full-length PREF-1 cDNA was isolated by iterative purification from a mixed-tissue cDNA library to which adipose contributed mRNA. Analysis of partial cDNAs from this library revealed that the 3'-terminal exon of the bovine PREF-1 mRNA is spliced in a manner analogous to its murine ortholog. However, we have also detected a PREF-1 splice form apparently unique to cattle. Aside from this alternative selection of a splice donor in the bovine fifth exon, the exon/intron junctions of the bovine PREF-1 gene recapitulate those observed for mice. The sequences proximal to the bovine PREF-1 transcription start site are homologous to the mouse PREF-1 promoter. Importantly, the sequence experimentally identified as critical to PREF-1 "suppression in adipocyte differentiation" is conserved in the bovine gene. The bovine PREF-1 gene was mapped to the telomeric end of BTA 21 by virtue of a physically linked microsatellite with seven alleles and 285 informative meiosis.
Fahrenkrug
S C
SC
U.S. Department of Agriculture, Agriculture Research Service, U.S. Meat Animal Research Center, Clay Center, Nebraska, 68933-0166, USA. fahrenkrug@email.marc.usda.gov
Freking
B A
BA
Smith
T P
TP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002417:Cattle; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D016678:Genome; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins