title: A novel model to study the dorsolateral migration of melanoblasts.
abstract: Melanocytes derived from pluripotent neural crest cells migrate initially in the dorsolateral pathway between the ectoderm and dermomyotome. To understand the role of specific proteins involved in this cell migration, we looked for a cellular model that mimics the in vivo behavior of melanoblasts, and that allows functional studies of their migration. We report here that wild-type embryonic stem (ES) cells are able to follow the ventral and dorsolateral neural crest pathways after being grafted into chicken embryos. By contrast, a mutant ES cell line deficient for beta1 integrin subunits, proteins involved in cell-extracellular interactions, had a severely impaired migratory behavior. Interestingly, ES cells deficient for Kit, the tyrosine kinase receptor for the stem cell factor (SCF), behaved similarly to wild-type ES cells. Thus, grafting mouse ES cells into chicken embryos provides a new cellular system that allows both in vitro and in vivo studies of the molecular mechanisms controlling dorsolateral migration.
authors.affiliation: Developmental Genetics of Melanocytes, UMR 146 CNRS-Institut Curie, Bat. 110, 91405, Orsay, France.
authors.forename: A
authors.initials: A
authors.lastname: Beauvais-Jouneau
authors.forename: P
authors.initials: P
authors.lastname: Pla
authors.forename: F
authors.initials: F
authors.lastname: Bernex
authors.forename: S
authors.initials: S
authors.lastname: Dufour
authors.forename: J
authors.initials: J
authors.lastname: Salamero
authors.forename: R
authors.initials: R
authors.lastname: Fässler
authors.forename: J J
authors.initials: JJ
authors.lastname: Panthier
authors.forename: J P
authors.initials: JP
authors.lastname: Thiery
authors.forename: L
authors.initials: L
authors.lastname: Larue
mesh_terms: D000818:Animals; D001665:Binding Sites; D015415:Biomarkers; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics; D004627:Embryonic Induction; D019334:Endothelin-3 / Q000235:genetics; D005353:Fibronectins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics; D008544:Melanocytes / Q000502:physiology*; D008562:Membrane Glycoproteins; D051379:Mice; D008817:Mice, Mutant Strains; D051739:Microphthalmia-Associated Transcription Factor; D014442:Monophenol Monooxygenase / Q000235:genetics; D009154:Mutation; D009420:Nervous System / Q000166:cytology / Q000196:embryology; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000235:genetics; D000071250:Snail Family Transcription Factors; D033581:Stem Cell Transplantation; D014157:Transcription Factors / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004268:DNA-Binding Proteins; D019334:Endothelin-3; D005353:Fibronectins; D005456:Fluorescent Dyes; D019012:Integrin beta1; D008562:Membrane Glycoproteins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D011506:Proteins; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D010088:Oxidoreductases; C079621:Tyrp1 protein, mouse; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D019009:Proto-Oncogene Proteins c-kit; D001616:beta-Galactosidase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase