Bone growth on and resorption of calcium phosphate coatings obtained by pulsed laser deposition.
Three different calcium phosphate coatings of crystalline hydroxyapatite (HA), alpha- and beta-tricalcium phosphate (alpha+beta-TCP), or amorphous calcium phosphate (ACP) obtained by pulsed laser deposition on Ti-6Al-4V were incubated in a potentially osteogenic primary cell culture (rat bone marrow) in order to evaluate the amount and mode of mineralized bone matrix formation after 2 weeks with special emphasis on the type of interfacial structure that was created. Evaluation techniques included fluorescence labeling and scanning electron microscopy. The resistance to cellular resorption by osteoclasts was also studied. Bone matrix delaminated from the ACP coatings, while it remained on the HA and the alpha+beta-TCP coatings even after fracturing. A cementlike line was seen as the immediate contiguous interface with the nondegrading dense HA surface and with the surface of the remaining porous beta-TCP coating. Highly dense and crystalline HA coatings do not dissolve but are capable of establishing a strong bond with the bone matrix grown on top. Chemical and mechanical bonding were considered in this case. Cellular resorption was practically not observed on the HA coatings, but it was observed on the alpha+beta-TCP coatings. Resorption took place as dissolution that was due to the acidic microenvironment.
Clèries
L
L
Universitat de Barcelona, Departament de Física Aplicada i Optica, Avinguda Diagonal 647, E-08028 Barcelona, Spain. lcleries@fao.ub.es
Fernández-Pradas
J M
JM
Morenza
J L
JL
D000497:Alloys; D000818:Animals; D001846:Bone Development; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism; D001862:Bone Resorption / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002469:Cell Separation; D002686:Chitin / Q000031:analogs & derivatives; D048271:Chitosan; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D013754:Tetracyclines / Q000378:metabolism; D014025:Titanium; D014961:X-Ray Diffraction
D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; D013754:Tetracyclines; C485828:alpha-tricalcium phosphate; C519480:amorphous calcium phosphate; C485830:tetracalcium phosphate; C031462:titanium alloy (TiAl6V4); D002686:Chitin; C485838:calcium phosphate, monobasic, anhydrous; D048271:Chitosan; C020243:calcium phosphate; D014025:Titanium; C485829:calcium phosphate, dibasic, anhydrous