Molecular characterization of Streptococcus equi subspecies equi isolated from an Ethiopian camel by ribotyping and PCR-ribotyping.
The aim of this study was to characterize a S. equi subspecies equi strain isolated from an Ethiopian camel by different molecular techniques (Ribotyping and PCR-Ribotyping). We compared the results obtained with those generated from two strains of the Pasteur Collection. The ribotyping showed the highest power of differentiation, distinguishing between the strains analyzed, whereas PCR-Ribotyping was able only to differentiate the camel isolate but not the strains from the Pasteur Collection. The application of this technique will be very useful to establish a clonal relationship among equine and camelids strains and help the prevention and cure of the equine and camel pathology.
Sechi
L A
LA
Dipartimento di Scienze Biomediche, Università degli studi di Sassari, Italy.
Roger
F
F
Diallo
A
A
Yigezu
L M
LM
Zanetti
S
S
Fadda
G
G
D000818:Animals; D015373:Bacterial Typing Techniques; D002162:Camelus / Q000382:microbiology*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005002:Ethiopia; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D018502:Streptococcus equi / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012407:rRNA Operon
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D004262:DNA Restriction Enzymes