Glucose and amino acid metabolism in Caco-2 cells maintained in a serum- and hormone-free medium.
Glucose and amino acid metabolism in a human enterocyte-like cell line (Caco-2) cultured in a serum- and hormone-free medium was examined and effects of epidermal growth factor (EGF) on these cells determined. These cells took up glutamine and converted it to alanine and proline. EGF stimulated the metabolism significantly.
Suzuki
Y
Y
Nisshin Flour Milling Co., Ltd., Saitama, Japan.
Motoi
H
H
D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D018938:Caco-2 Cells; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006728:Hormones / Q000378:metabolism; D006801:Humans; D011392:Proline / Q000378:metabolism
D000596:Amino Acids; D016895:Culture Media, Serum-Free; D006728:Hormones; D005973:Glutamine; D004815:Epidermal Growth Factor; D011392:Proline; D005947:Glucose; D000409:Alanine