title: Borna disease virus infection in domestic cats: evaluation by RNA and antibody detection.
abstract: Borna disease virus (BDV) infection has been suggested to cause spontaneous neurological disease in cats referred to as staggering disease. However the evaluation of BDV infection in neurologically asymptomatic cats remained unclear. In the present study, BDV infected, asymptomatic cats in Tokyo were surveyed both by the presence of plasma antibodies against BDV-p24 and -p40 and by RNA detection in peripheral blood mononuclear cells. Seven of 32 domestic cats (21.9%) were serologically or genetically judged to be BDV-infected. Six cats were positive for anti-BDV antibody and two cats were positive for BDV RNA. Within the 2 RNA-positive cats, only one was positive for anti-BDV antibodies. Furthermore, the findings of anti-BDV-p40 and anti-BDV-p24 antibody-positive cats did not completely overlap. These results suggest that there are neurologically asymptomatic domestic cats infected with BDV present in the Tokyo area.
authors.affiliation: Department of Nutrition, School of Veterinary Medicine, Azabu University, Sagamihara, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nishino
authors.forename: M
authors.initials: M
authors.lastname: Funaba
authors.forename: R
authors.initials: R
authors.lastname: Fukushima
authors.forename: T
authors.initials: T
authors.lastname: Mizutani
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: R
authors.initials: R
authors.lastname: Iizuka
authors.forename: H
authors.initials: H
authors.lastname: Hirami
authors.forename: M
authors.initials: M
authors.lastname: Hara
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015139:Blotting, Southern / Q000662:veterinary; D015153:Blotting, Western / Q000662:veterinary; D001890:Borna Disease / Q000276:immunology*; D001891:Borna disease virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002371:Cat Diseases / Q000276:immunology / Q000821:virology*; D002415:Cats; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005260:Female; D008297:Male; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D012367:RNA, Viral