title: MboII endonuclease heat inactivation before agarose gel electrophoresis to prevent artifactual bands in restriction patterns.
authors.affiliation: Unité des Entérobactéries, Institut Pasteur, Paris, France.
authors.forename: G M
authors.initials: GM
authors.lastname: Giammanco
authors.forename: F
authors.initials: F
authors.lastname: Grimont
authors.forename: P A
authors.initials: PA
authors.lastname: Grimont
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D012150:Polymorphism, Restriction Fragment Length; D011489:Protein Denaturation
chemical_list: D004269:DNA, Bacterial; C024413:endodeoxyribonuclease MboII; D015252:Deoxyribonucleases, Type II Site-Specific