Characterization of an alkali-extracted peptidoglycan from Korean Ganoderma lucidum.
The biologically active peptidoglycan was purified from the alkali fraction of the fruiting bodies of Ganoderma lucidum and the composition of the peptidoglycan was investigated by conventional analyses. The alkali-extracted peptidoglycan showed differences in chemical compositions from the water-extracted. The alkali-extracted peptidoglycan contained 6.9% protein and 75.9% carbohydrates composed mainly of beta-glucose, mannose, and alpha-glucose. The molecular weight range of the peptidoglycan was determined as 2,000 kDa-17 kDa. The peptidoglycan is considered to be a hybrid molecule of polysaccharide chains covalently bound as a side chain to the polypeptide core.
Cheong
J
J
IlYang Central Research Institute, Yongin, Kyungki, Korea.
Jung
W
W
Park
W
W
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D005947:Glucose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D020940:Reishi / Q000737:chemistry*; D012972:Sodium Hydroxide; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
D000596:Amino Acids; D002241:Carbohydrates; D009005:Monosaccharides; D010457:Peptidoglycan; D010936:Plant Extracts; D011506:Proteins; D012972:Sodium Hydroxide; D005947:Glucose; D008358:Mannose