In situ RT-PCR allows the detection of ornithine decarboxylase mRNA in paraffin embedded archival human hyperplastic breast tissues.
Expression of ornithine decarboxylase (ODC) is induced by c-Myc oncoprotein and is required for cell proliferation and tumour growth. We have studied the expression of ODC mRNA by in situ hybridisation and in situ RT-PCR in archival human hyperplastic breast tissues. A very low signal was detected by in situ hybridisation, while the in situ RT-PCR on human breast archival tissues demonstrated an over-expression of ODC mRNA in epithelial cells characterised by some degree of hyperplasia, maintaining the morphology of the archival tissue intact despite the multiple steps of fixation, permeabilization and thermal cycling.
Clerico
L
L
Dipartimento di Oncologia Sperimentale, Università di Genova, Italy.
Mancuso
T
T
Da Prato
L
L
Marziliano
N
N
Garagna
S
S
Pecile
V
V
Demori
E
E
Morgutti
M
M
Città
A
A
Parodi
S
S
Amoroso
A
A
Crovella
S
S
D001940:Breast / Q000201:enzymology* / Q000473:pathology*; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D017403:In Situ Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D016612:Paraffin Embedding; D010539:Permeability; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016707:Tissue Fixation
D012333:RNA, Messenger; D009955:Ornithine Decarboxylase