Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana.
A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2'-deacetoxydecinnamoyltaxinine J and 2'-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV-Vis as well as photodiode array detection.
Bala
S
S
Analytical Instrumentation, Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Uniyal
G C
GC
Chattopadhyay
S K
SK
Tripathi
V
V
Sashidhara
K V
KV
Kulshrestha
M
M
Sharma
R P
RP
Jain
S P
SP
Kukreja
A K
AK
Kumar
S
S
D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017239:Paclitaxel / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014197:Trees / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel