title: Extracellular mannose-6-phosphatase of Phanerochaete chrysosporium: a lignin peroxidase-modifying enzyme.
abstract: The lignin peroxidase (LIP) isozyme profile of the white-rot fungus Phanerochaete chrysosporium changes markedly with culture age. This change occurs extracellularly and results from enzymatic dephosphorylation of LIP isozymes. In this study, a novel mannose 6-phosphatase (M6Pase) from extracellular culture fluid filtrate of P. chrysosporium, shown to be responsible for the extracellular postranslational modification of LIP, was purified and characterized. In vitro incubation of the purified M6Pase with purified LIP isozyme H2 resulted in its conversion to isozyme H1, with an equimolar release of orthophosphate. Using different sugar phosphates as substrate, the enzyme exhibited narrow specificity, showing activity mostly for mannose 6-phosphate (K(m) = 0.483 mM). The enzyme displayed a molecular mass of 82 kDa, as determined by gel filtration, and 40.4 and 39.1 kDa, on SDS-PAGE, suggesting that the native form is a dimer. The N-terminal sequence of the enzyme has no homology with that of other reported phosphatases. M6Pase is a metalloprotein with manganese and cobalt as the preferred metal ions. It is N-glycosylated proteins with an isoelectric point of 4. 7-4.8 and a pH optimum of 5. Based on its characteristics, M6Pase from P. chrysosporium seems to be a unique phosphatase responsible for posttranslation modification of LIP isozymes.
authors.affiliation: MIGAL-Galilee Technology Center, Kiryat Shmona, South Industrial Zone, 10200, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Rothschild
authors.forename: A
authors.initials: A
authors.lastname: Levkowitz
authors.forename: Y
authors.initials: Y
authors.lastname: Hadar
authors.forename: C
authors.initials: C
authors.lastname: Dosoretz
mesh_terms: D000595:Amino Acid Sequence; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D008670:Metals; D010544:Peroxidases; C042858:lignin peroxidase; C023553:mannose-6-phosphatase; D010744:Phosphoric Monoester Hydrolases