title: Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells.
abstract: The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14C-L1 at 37 degrees C. Following incubation with 0.5 mM 14C-L1 for 4 h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 +/- 2% of 14C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol.
authors.affiliation: Department of Medicine, Hadassah University Hospital, Jerusalem, Israel. odeds@cc.huji.ac.il
authors.forename: O
authors.initials: O
authors.lastname: Shalev
authors.forename: D
authors.initials: D
authors.lastname: Hileti
authors.forename: P
authors.initials: P
authors.lastname: Nortey
authors.forename: R P
authors.initials: RP
authors.lastname: Hebbel
authors.forename: V A
authors.initials: VA
authors.lastname: Hoffbrand
mesh_terms: D000328:Adult; D000755:Anemia, Sickle Cell / Q000378:metabolism*; D001692:Biological Transport; D000077543:Deferiprone; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007502:Iron Chelating Agents / Q000493:pharmacokinetics*; D011728:Pyridones / Q000493:pharmacokinetics*; D013789:Thalassemia / Q000378:metabolism*
chemical_list: D007502:Iron Chelating Agents; D011728:Pyridones; D000077543:Deferiprone