Evaluation of radical products from beta-alanine/sugar mixtures by use of GC-MS with the galvinoxyl radical.
We have quantified the radical production of Maillard systems with beta-alanine and different sugars by a method that uses the free radical scavenger capacity of galvinoxyl (2,6-di-tert-butyl-alpha-(3, 5-di-tert-butyl-4-oxo-2,5-cyclohexadiene-1-ylidene)-p-tolyloxy) and a GC-MS separation and quantification. We have compared the results with the spectrophotometrical conclusions and highlight the importance of the radical production in the Maillard systems. The method is precise (CV < 5%), accurate (>97%), sensitive (limit of detection 10 ng/mL galvinoxyl), and may be used for the detection and quantification of radical activity in the chemical and biological systems.
Georgescu
L P
LP
Department of Chemistry, University of Galati, Romania.
Georgescu
M E
ME
Leonte
M
M
Florea
T
T
D001559:Benzhydryl Compounds; D004187:Disaccharides / Q000737:chemistry*; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015416:Maillard Reaction; D009005:Monosaccharides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D015091:beta-Alanine / Q000737:chemistry*
D001559:Benzhydryl Compounds; D004187:Disaccharides; D016166:Free Radical Scavengers; D005609:Free Radicals; D009005:Monosaccharides; D015091:beta-Alanine; C020338:galvinoxyl