title: Groalpha-mediated recruitment of neutrophils is required for elicitation of contact hypersensitivity.
abstract: The factors mediating recruitment of immune T cells to challenge sites during contact hypersensitivity (CHS) responses remain unclear. To investigate the role of chemokines during elicitation of CHS, the temporal expression of chemokine genes in hapten-challenged ears was tested. KC (the murine homologoue of Groalpha) was expressed 30 min following hapten challenge in naive and hapten-sensitized mice. A rabbit KC-specific antiserum inhibited elicitation of CHS when administered to sensitized mice prior to hapten challenge. Injecting either neutrophils or immune CD8(+) T cells into the ear tissue of immune animals before hapten challenge circumvented the KC antiserum-mediated inhibition of CHS. Neutrophil depletion also inhibited CHS and was circumvented by injecting either neutrophils or hapten-primed CD8(+) T cells into ears of sensitized mice followed by specific hapten challenge. These results indicate that KC-directed neutrophil infiltration of hapten challenge sites is required for elicitation of CHS and suggest that neutrophils mediate recruitment of the hapten-specific CD8(+) T cells that subsequently produce cytokines mediating the hypersensitivity response.
authors.affiliation: Department of Immunology, Cleveland Clinic Foundation, Cleveland, USA.
authors.forename: N A
authors.initials: NA
authors.lastname: Dilulio
authors.forename: T
authors.initials: T
authors.lastname: Engeman
authors.forename: D
authors.initials: D
authors.lastname: Armstrong
authors.forename: C
authors.initials: C
authors.lastname: Tannenbaum
authors.forename: T A
authors.initials: TA
authors.lastname: Hamilton
authors.forename: R L
authors.initials: RL
authors.lastname: Fairchild
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000276:immunology*; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D006133:Growth Substances / Q000096:biosynthesis / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*; D011817:Rabbits
chemical_list: D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C475895:Cxcl1 protein, mouse; D006133:Growth Substances; D006241:Haptens; D036341:Intercellular Signaling Peptides and Proteins