Inhibition of Df-protease associated with allergic diseases by polyphenol.
It was reported that Df-protease from house dust mite (Dermatophagoides farinae) catalyzes the activation of the kallikrein-kinin system in human plasma and is closely associated with mite-induced allergy. Therefore, to prevent the release of kinin by Df-protease, the inhibitory activity of polyphenols including catechins and flavonols was tested in vitro and in vivo. Among them, myricetin and epigallocatechin gallate (EGCg) effectively inhibited the amidase activity of Df-protease with K(i) values of 1 x 10(-)(8) and 6 x 10(-)(4) M, respectively. The kinin release in human plasma was extensively inhibited by the addition of EGCg in comparison with myricetin. Enhancement of vascular permeability in guinea pigs caused by Df-protease was markedly suppressed by EGCg.
Noguchi
Y
Y
Toin Human Science and Technology Center, Department of Material Science and Technology, Toin University of Yokohama, Aoba-ku, Japan.
Fukuda
K
K
Matsushima
A
A
Haishi
D
D
Hiroto
M
M
Kodera
Y
Y
Nishimura
H
H
Inada
Y
Y
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D006967:Hypersensitivity / Q000517:prevention & control*; D007700:Kinetics; D007705:Kinins / Q000097:blood; D008925:Mites / Q000201:enzymology* / Q000276:immunology*; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012697:Serine Endopeptidases / Q000276:immunology* / Q000378:metabolism
D005419:Flavonoids; D007705:Kinins; D010636:Phenols; D011108:Polymers; D011480:Protease Inhibitors; D002392:Catechin; C045651:epigallocatechin gallate; C099397:Df protease; D012697:Serine Endopeptidases