title: A new monoclonal antibody detects a developmentally regulated mouse ecto-ADP-ribosyltransferase on T cells: subset distribution, inbred strain variation, and modulation upon T cell activation.
abstract: ADP-ribosylation of membrane proteins on mouse T cells by ecto-ADP-ribosyltransferase(s) (ARTs) can down-regulate proliferation and function. The lack of mAbs against mouse ARTs has heretofore prevented analysis of ART expression on T cell subsets. Using gene gun technology, we immunized a Wistar rat with an Art2b expression vector and produced a novel mAb, Nika102, specific for ART2.2, the Art2b gene product. We show that ART2.2 is expressed as a GPI-anchored protein on the surface of mature T cells. Inbred strain-dependent differences in ART2.2 expression levels were observed. C57BL/6J and C57BLKS/J express the Ag at high level, with up to 70% of CD4+ and up to 95% of CD8+ peripheral T cells expressing ART2.2. CBA/J and DBA/2J represent strains with lowest expression levels. T cell-deficient mice and NZW/LacJ mice with a defective structural gene for this enzyme were ART2.2 negative. In the thymus, ART2.2 expression is restricted to subpopulations of mature cells. During postnatal ontogeny, increasing percentages of T cells express ART2.2, reaching a peak at 6-8 wk of age. Interestingly, ART2.2 and CD25 are reciprocally expressed: activation-induced up-regulation of CD25 is accompanied by loss of ART2.2 from the cell surface. Nika102 thus defines a new differentiation/activation marker of thymic and postthymic T cells in the mouse and should be useful for further elucidating the function of the ART2.2 cell surface enzyme.
authors.affiliation: Institute for Immunology, University Hospital, Hamburg, Germany; The Jackson Laboratory, Bar Harbor, ME 04609, USA. nolte@uke.uni-hamburg.de
authors.forename: F
authors.initials: F
authors.lastname: Koch-Nolte
authors.forename: T
authors.initials: T
authors.lastname: Duffy
authors.forename: M
authors.initials: M
authors.lastname: Nissen
authors.forename: S
authors.initials: S
authors.lastname: Kahl
authors.forename: N
authors.initials: N
authors.lastname: Killeen
authors.forename: V
authors.initials: V
authors.lastname: Ablamunits
authors.forename: F
authors.initials: F
authors.lastname: Haag
authors.forename: E H
authors.initials: EH
authors.lastname: Leiter
mesh_terms: D036002:ADP Ribose Transferases; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D015536:Down-Regulation; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology*; D009842:Oligopeptides; D010455:Peptides; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000276:immunology*; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000201:enzymology*; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D006649:Histocompatibility Antigens; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009842:Oligopeptides; D010455:Peptides; D011994:Recombinant Proteins; C085706:FLAG peptide; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; C449322:Art2a protein, mouse; C090163:Art2b protein, rat; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase