N(6)-Furfuryladenine, kinetin, protects against Fenton reaction-mediated oxidative damage to DNA.
N(6)-Furfuryladenine (kinetin) has been shown to have anti-ageing effects on several different systems including plants, human cells in culture, and fruitflies. Since most of the experimental data point toward kinetin acting as an antioxidant both in vitro and in vivo, and since much evidence supporting a causal role of oxidative damage in ageing is accumulating, we tested the antioxidant properties of kinetin directly. Using 8-oxo-2'deoxyguanosine (8-oxo-dG) in calf thymus DNA as a marker for oxidative damage, we demonstrate that kinetin significantly (P < 0.005) protects the DNA against oxidative damage mediated by the Fenton reaction. Kinetin inhibited 8-oxo-dG formation in a dose-dependent manner with a maximum of 50% protection observed at 100 microM kinetin.
Olsen
A
A
Laboratory of Cellular Ageing, Department of Molecular and Structural Biology, University of Aarhus, Gustav Wieds Vej 10-C, Aarhus, DK-8000, Denmark.
Siboska
G E
GE
Clark
B F
BF
Rattan
S I
SI
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D004247:DNA / Q000187:drug effects* / Q000378:metabolism*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D066298:In Vitro Techniques; D007701:Kinetin; D008954:Models, Biological; D010084:Oxidation-Reduction
D000975:Antioxidants; D016166:Free Radical Scavengers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine; D000225:Adenine; D007701:Kinetin