title: Ion-pair partition of quarternary ammonium drugs: the influence of counter ions of different lipophilicity, size, and flexibility.
abstract: OBJECTIVE
  The ion-pair partition of quaternary ammonium (QA) pharmacons with organic counter ions of different lipophilicity, size, shape and flexibility was studied to elucidate relationships between ion-pair formation and chemical structure.
  
  
  METHODS
  The apparent partition coefficient (P') of 4 QAs was measured in octanol/pH 7.4 phosphate buffer system by the shake-flask method as a function of molar excess of ten counter ions (Y), namely: mesylate (MES), acetate (AC), pyruvate (PYRU), nicotinate (NIC), hydrogenfumarate (HFUM), hydrogenmaleate (HMAL), p-toluenesulfonate (PTS), caproate (CPR), deoxycholate (DOC) and prostaglandin E1 anion (PGE1).
  
  
  RESULTS
  Based on 118 of highly precise logP' values (SD< 0.05), the intrinsic lipophilicity (without external counter ions) and the ion-pair partition of QAs (with different counter ions) were characterized. Linear correlation was found between the logP' of ion-pairs and the size of the counter ions described by the solvent accessible surface area (SASA). The lipophilicity increasing effect of the counter ions were quantified and the following order was established: DOC approximate to PGE1 >> CPR approximate to PTS >> NIC approximate to HMAL >> PYRU approximate to AC approximate to MES approximate to HFUM. Analyzing the lipophilicity/molar ratio (QA:Y) profile, the differences in the ion-pair formation were shown and attributed to the differences in the flexibility/rigidity and size both of QA and Y.
  
  
  CONCLUSIONS
  Since the largest (in average, 300 X) lipophilicity enhancement was found by the influence of DOC and PGE1 and considerable (on average 40 X) increase was observed by CPR and PTS, it was concluded that bile acids and prostaglandin anions may play a significant role in the ion-pair transport of quaternary ammonium drugs and caproic acid and p-toluenesulfonic acid may be useful salt forming agents to improve the pharmacokinetics of hydrophilic drugs.
authors.affiliation: Institute of Pharmaceutical Chemistry, Semmelweiss University of Medicine, Budapest, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Takács-Novák
authors.forename: G
authors.initials: G
authors.lastname: Szász
mesh_terms: D000042:Absorption; D000465:Algorithms; D000527:Alprostadil / Q000737:chemistry; D002208:Caproates / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003840:Deoxycholic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008970:Molecular Weight; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012997:Solvents
chemical_list: D002208:Caproates; D008055:Lipids; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D003840:Deoxycholic Acid; D000527:Alprostadil