title: Evidence for autoregulation of cystathionine gamma-synthase mRNA stability in Arabidopsis.
abstract: Control of messenger RNA (mRNA) stability serves as an important mechanism for regulating gene expression. Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Transfection experiments with wild-type and mutant forms of the CGS gene suggest that an amino acid sequence encoded by the first exon of CGS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.
authors.affiliation: Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Chiba
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: F
authors.initials: F
authors.lastname: Kijima
authors.forename: R H
authors.initials: RH
authors.lastname: Tyson
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: E
authors.initials: E
authors.lastname: Nambara
authors.forename: T
authors.initials: T
authors.lastname: Leustek
authors.forename: R M
authors.initials: RM
authors.lastname: Wallsgrove
authors.forename: S
authors.initials: S
authors.lastname: Naito
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D019757:Carbon-Oxygen Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D008715:Methionine; C022696:O-succinylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases