Inhibition of carcinogen-induced activity of gamma-glutamyl transpeptidase by certain dietary constituents in mouse skin.
Cancer chemoprevention, a desirable and important facet of biomedical research, provides a practical approach to identify potentially useful inhibitors of cancer development, and offers an opportunity to study the mechanism of carcinogenesis. During the recent past a number of compounds have been tested for their anticarcinogenic potential specially constituents of human diet. The enzyme gamma-glutamyl transpeptidase (GGT), which catalyses the transfer of glutamyl groups of peptides to other peptides and amino acids, has been proposed as a marker of cell proliferation and neoplasia. It also serves as a tool to evaluate the carcinogenic and cocarcinogenic potential of environmental toxicants. In the present investigations, GGT activity induced by carcinogenic polycyclic aromatic hydrocarbons, viz. 7,12-dimethyl-benz(a)anthracene (DMBA) and benzo(a)pyrene (BaP) was significantly inhibited by diallylsulfide (DAS) and indole-3-carbinol (I3C) in mouse skin. DAS and I3C are constituents of garlic. A significant inhibition in GGT levels was also observed in a strong mitogen (12-o-tetradecanoyl phorbol-13-acetate) induced activity by pretreatment of DAS/I3C in mouse skin. Therefore these dietary constituents seem to be strong modifiers of chemically induced carcinogenesis.
Shukla
Y
Y
Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Lucknow, India.
Singh
A
A
Srivastava
B
B
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D004032:Diet; D005260:Female; D005737:Garlic / Q000737:chemistry; D006841:Hydrocarbons, Aromatic / Q000378:metabolism / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D010946:Plants, Medicinal; D013440:Sulfides / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000187:drug effects / Q000378:metabolism*
D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D002273:Carcinogens; D006841:Hydrocarbons, Aromatic; D007211:Indoles; D013440:Sulfides; C038491:allyl sulfide; C016517:indole-3-carbinol; D005723:gamma-Glutamyltransferase