The basal-level expression of the cytochrome P450(BM-1) gene is negatively affected by the bm1P1 gene of Bacillus megaterium.
Evidence against the Bm1P1 protein as a positive transcription factor for barbiturate-mediated induction of cytochrome P450(BM-1) in Bacillus megaterium has been provided in our previous report. In the present study, we show that the basal-level expression of the P450(BM-1) promoter-xylE transcriptional fusion is significantly reduced when the bm1P1 gene is present on the same plasmid. Moreover, disruption of the chromosomal bm1P1 gene results in enhanced expression of the P450(BM-1) promoter-xylE fusion on a multicopy plasmid. By using a binary vector system, we found that expression of the P450(BM-1) promoter-xylE fusion from a high-copy plasmid is substantially repressed by expression of bm1P1 from a low-copy plasmid. Taken together, these results suggest that the basal-level expression of P450(BM-1) is negatively affected by bm1P1. Possible mechanisms for this control are discussed.
Shaw
G C
GC
Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China.
Hsueh
Y H
YH
Kao
H S
HS
D000818:Animals; D001410:Bacillus megaterium / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D049308:Dioxygenases; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
C094974:BM1P1 protein, Bacillus megaterium; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C073493:Bm3R1 protein, Bacillus megaterium; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase