Antisense methods for discrimination of phenotypic properties of closely related gene products: Jun kinase family.
Methods for the selection and characterization of antisense oligonucleotides for specifically eliminating closely related gene family members are available. High-throughput semiautomated methods using 96-well plate formats and array technology and improved assays are under active development that will streamline many steps and will likely merge. Second-generation 20-mer antisense phosphorothioate oligonucleotides containing 2'-methoxyethyl groups at the first and last 6 nucleotides with improved nuclease resistance and RNA affinity are becoming available.
Bost
F
F
Sidney Kimmel Cancer Center, San Diego, California 92121, USA.
McKay
R
R
Dean
N M
NM
Potapova
O
O
Mercola
D
D
D000818:Animals; D002471:Cell Transformation, Neoplastic; D015870:Gene Expression / Q000187:drug effects; D006207:Half-Life; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases; D008081:Liposomes; D051379:Mice; D018345:Mice, Knockout; D048057:Mitogen-Activated Protein Kinase 10; D048056:Mitogen-Activated Protein Kinase 9; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010641:Phenotype; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology; D014162:Transfection; D014183:Transplantation, Heterologous
D007527:Isoenzymes; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D011494:Protein Kinases; D048057:Mitogen-Activated Protein Kinase 10; D048056:Mitogen-Activated Protein Kinase 9; D011505:Protein-Tyrosine Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases