title: Fluorescent Indicators for Imaging Nitric Oxide Production.
abstract: The membrane-permeating indicator DAF-FM DA is transformed by intracellular esterases into the highly water-soluble dye DAF-FM, which traps NO produced by NO synthase (NOS) to yield a highly fluorescent triazole compound in cells (see schematic diagram). Monitoring with a fluorescence microscope should allow direct identification of intracellular production and location of NO.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan).
authors.initials: H
authors.lastname: Kojima
authors.initials: Y
authors.lastname: Urano
authors.initials: K
authors.lastname: Kikuchi
authors.initials: T
authors.lastname: Higuchi
authors.initials: Y
authors.lastname: Hirata
authors.initials: T
authors.lastname: Nagano