title: Medicinal yeast extracts.
abstract: Alcoholic extracts of bakers' yeast (Saccharomyces cerevisiae) have been used for over 60 years in over-the-counter medications for the treatment of hemorrhoids, burns, and wounds. Although previous studies suggested that small peptides were responsible for the medical observations, the peptides were never resolved into separate fractions and identified. In the present report, a protein fraction was prepared by RPC18 chromatography of the extract which enhances wound closure in both diabetic and non-diabetic littermates. The peptides are active in nanomolar amounts and are 600 times more active than the initial extract. SDS-PAGE and N-terminal amino acid sequencing identified 4 polypeptides in the extract. Three of the proteins were small molecular weight stress-associated proteins: copper, zinc superoxide-dismutase, ubiquitin, and glucose lipid regulated protein (HSP 12). The fourth protein, acyl-CoA binding protein II, has not been previously associated with stress proteins.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Ohio 45221-0006, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Schlemm
authors.forename: M J
authors.initials: MJ
authors.lastname: Crowe
authors.forename: R B
authors.initials: RB
authors.lastname: McNeill
authors.forename: A E
authors.initials: AE
authors.lastname: Stanley
authors.forename: S J
authors.initials: SJ
authors.lastname: Keller
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D026261:Diazepam Binding Inhibitor; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D014452:Ubiquitins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D014947:Wounds and Injuries / Q000188:drug therapy
chemical_list: D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D016166:Free Radical Scavengers; D005656:Fungal Proteins; C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D014452:Ubiquitins; D013482:Superoxide Dismutase