Modulation by Anapsos (Polypodium leucotomos extract) of the antibody responses against the nematode parasite Trichinella spiralis.
The immunomodulant effects of Anapsos, an extract of the naturally occurring fern Polypodium leucotomos was assessed in Balb/c mice immunized with a crude soluble extract (CSE) of Trichinella spiralis L1 larvae. Treatment from day 10 to 1 prior to immunization caused a significant reduction (p < 0.05) in total antibody levels (IgG + M) that was evident from week 2 onwards. Suppression of the IgG1 response was transient, as serum levels were significantly (p < 0. 01) decreased in treated animals during weeks 2 and 3 post-immunization, afterwards increasing to values similar to those in the control group. An opposite pattern was observed in the IgG2a and IgG 2b profiles where, following a brief increase in weeks 3 and 1, respectively, the values fell below those of the control and remained for the whole observation. Anapsos potentiates the IgG3 response against T. spiralis CSE. Deglycosylation of the CSE used in the ELISA assay significantly reduces the IgG3 recognition capacity in both control and treated mice. This treatment did not affect the time-course of an intestinal infection by T. spiralis.
Dea-Ayuela
M
M
Department of Parasitology, Faculty of Pharmacy, Complutense University, 28040-Madrid, Spain.
Rodero
M
M
Rodriguez-Bueno
R
R
Bolás-Fernández
F
F
Martínez-Fernández
A R
AR
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006027:Glycosides / Q000494:pharmacology*; D006031:Glycosylation; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D017160:Trichinella spiralis / Q000187:drug effects* / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D006027:Glycosides; D007074:Immunoglobulin G; D010936:Plant Extracts; C006870:anapsos