The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo.
This report examines the use of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) to determine the site, duration and cell type responsible for antigen presentation in vivo. Evidence that CFSE-labelled T cells can be used to determine where various types of antigens are presented, including auto-antigens, oral antigens and cell-associated foreign antigens, is provided. Using this technique, the length of time antigen is presented after acquisition by APC was measured. Finally, CFSE labelling was used to identify the origin of the APC responsible for different forms of antigen presentation.
Mintern
J
J
Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria.
Li
M
M
Davey
G M
GM
Blanas
E
E
Kurts
C
C
Carbone
F R
FR
Heath
W R
WR
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007108:Immune Tolerance / Q000276:immunology; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D001324:Autoantigens; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides