Biological properties of opioid peptides replacing Tyr at position 1 by 2,6-dimethyl-Tyr.
To understand the effect of the replacement of Tyr residue at position 1 in opioid peptides by 2,6-dimethyl-Tyr (Dmt) on the biological property, chiral (D or L) Dmt1 analogs of Leu-enkephalin (Enk) and Tyr-D-Arg-Phe-beta Ala-NH2 (YRFB) were synthesized and their enzymatic stabilities, in vitro bioactivities and receptor binding affinities compared with those of parent peptides. [L-Dmt1]Enk (1) exhibited 4-fold higher stability against aminopeptidase-M and possessed dramatically increased activities in guinea pig ilium (GPI) (187-fold) and mouse vas deferens (MVD) (131-fold) assays, and in rat brain receptor binding assays (356-fold at mu receptor and 46-fold at delta receptor) as compared to Enk. [L-Dmt1]YRFB (3) also exhibited increased activities in GPI (46-fold) and MVD (177-fold) assays, and in the binding assays (69-fold at mu receptro and 341-fold at delta receptor) as compared to the parent peptide. [D-Dmt1]Enk (2) and [D-Dmt1]YRFB (4) exhibited activities with diminished or lesser potency than the parent peptide in all assays. These results indicate that there is a tendency for mu affinity to be enhanced more than delta affinity with introduction of L-Dmt into delta ligand peptide (Enk), and for delta affinity to be enhanced more than mu affinity in case of mu ligand peptide (YRFB), resulting in reduced receptor selectivities at the receptors.
Sasaki
Y
Y
Tohoku Pharmaceutical University, Sendai, Japan.
Suto
T
T
Ambo
A
A
Ouchi
H
H
Yamamoto
Y
Y
D000818:Animals; D004743:Enkephalin, Leucine / Q000737:chemistry; D006168:Guinea Pigs; D007536:Isomerism; D051379:Mice; D018847:Opioid Peptides / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism*
D018847:Opioid Peptides; D011957:Receptors, Opioid; C076121:2',6'-dimethyltyrosine; D014443:Tyrosine; D004743:Enkephalin, Leucine