title: [Tetraprotomeric hypothesis of Na/K-ATPase].
abstract: Since the discovery of Na/K-ATPase by Skou, the mechanism of Na/K-dependent ATP hydrolysis and Na and K transport has been extensively studied. The hydrolysis appears to occur sequentially via the Na-Enzyme-ATP complex (NaE1ATP), ADP-sensitive phosphoenzyme (NaE1P), the K-sensitive phosphoenzyme (E2P) and the K-occluded enzyme (KE2), known the Post-Albers mechanism, in a protomer or diprotomer that consists of alpha- and beta-chains. The tetrameric nature of the enzyme such as a quarter, half, third to fourth and full site reactivity and the visualization by electron microscopy show direct biochemical evidence for the presence of a tetraprotomer structure of Na/K-ATPase during ATP hydrolysis. ATP binding is followed by two parallel paths, which occur at each of the two half sites for phosphorylation-dephosphorylation, and direct ATP hydrolysis via (NaE1P : E.ATP)2, (E2P : E.ATP : E2P : E.ADP/Pi) and (KE2 : E.ADP/Pi)2, respectively. The sequential formation of E2P from NaE1P and KE2 from E2P is accompanied by, respectively, hydrolysis of half of the TCA-labile bound ATP to ADP/Pi and of another half of the bound ATP to ADP/Pi. All reaction intermediates detectable in the Post-Albers scheme bind ATP and/or ADP/Pi.
authors.affiliation: Division of Chemistry, Graduate School of Science Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: S
authors.initials: S
authors.lastname: Kaya
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: K
authors.initials: K
authors.lastname: Abe
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D006868:Hydrolysis; D010042:Ouabain / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase
chemical_list: D010042:Ouabain; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012964:Sodium; D006005:Phosphorylases; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium