title: Identification of three new alternate human kallikrein 2 transcripts: evidence of long transcript and alternative splicing.
abstract: In a search for prostate-specific genes in the human expressed sequence tag (EST) database, we identified a seemingly unique EST cluster C81. Experimental data linked C81 to the human hKLK2 gene that encodes a prostate specific serine protease-human glandular kallikrein (hK2). We uncovered a full-length hKLK2 cDNA corresponding to a 3.0 kb hKLK2 mRNA by PCR and sequence analysis. The 3.0 kb transcript accounts for about 25% of the hKLK2 transcripts as compared to the previously known 1.5 kb transcript. We also identified a third spliced form of the hKLK2 gene produced by alternative splicing between intron III and exon 4. This spliced form was detected in normal prostate, prostate cancer and the prostate adenocarcinoma cell line LNCaP. The identification of long hKLK2 transcript and an alternative spliced form of the hKLK2 gene indicates that regulation of the gene is complex.
authors.affiliation: Laboratory of Molecular Biology, Division of Basic Sciences, National Institutes of Health, Building 37, Room 4E16, Bethesda, Maryland, 20892, USA.
authors.forename: X F
authors.initials: XF
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Essand
authors.forename: G
authors.initials: G
authors.lastname: Vasmatzis
authors.forename: B
authors.initials: B
authors.lastname: Lee
authors.forename: I
authors.initials: I
authors.lastname: Pastan
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D020840:Tissue Kallikreins / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D020840:Tissue Kallikreins