The tissue distribution of the human beta3-adrenoceptor studied using a monoclonal antibody: direct evidence of the beta3-adrenoceptor in human adipose tissue, atrium and skeletal muscle.
OBJECTIVE
To develop a monoclonal antibody that recognises an epitope of the native beta3-adrenoceptor expressed on the extracellular surface of human cells and tissues.


METHODS
A high affinity monoclonal antibody, Mab72c, was raised against the human beta3-adrenoceptor expressed on a transfected mammalian cell line.


RESULTS
In CHO (Chinese hamster ovary) cells transfected with beta3-adrenoceptor cDNA, antibody labelling was found to be proportional to receptor density measured by the binding of the radiolabelled beta-adrenoceptor antagonist, [125I]-iodocyanopindolol. The use of Mab 72c has demonstrated the expression of the beta3-adrenoceptor in a variety of human tissues, including gall bladder, prostate and colon, where a mRNA signal had been detected previously. This study also provides the first direct demonstration of the expression of beta3-adrenoceptors in human skeletal muscle, atrium and adipose tissue.


CONCLUSIONS
The development of this antibody represents an important addition to the armentarium of reagents that are available to study the localisation of beta3-adrenoceptors in human tissues.
Chamberlain
P D
PD
Biotechnology Department, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
Jennings
K H
KH
Paul
F
F
Cordell
J
J
Berry
A
A
Holmes
S D
SD
Park
J
J
Chambers
J
J
Sennitt
M V
MV
Stock
M J
MJ
Cawthorne
M A
MA
Young
P W
PW
Murphy
G J
GJ
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal; D016466:CHO Cells; D006224:Cricetinae; D005434:Flow Cytometry; D006325:Heart Atria / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol / Q000378:metabolism; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000737:chemistry*; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000235:genetics; D022702:Receptors, Adrenergic, beta-3; D014018:Tissue Distribution; D014162:Transfection
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D020120:Iodocyanopindolol