title: Oxidative stress resistance of Escherichia coli strains deficient in glutathione biosynthesis.
abstract: Sensitivity to various oxidants was determined for Escherichia coli strains JTG10 and 821 deficient in biosynthesis of glutathione (gsh-) and their common parental strain AB1157 (gsh+). The three strains showed identical sensitivity to H2O2. E. coli 821 was more resistant than AB1157 and JTG10 to menadione, cumene hydroperoxide, and N-ethylmaleimide. This resistance was not related to the gsh mutation because the other gsh- mutant and the parental strain showed similar sensitivity to these oxidants. The measured activities of NADPH:menadione diaphorase and glucose-6-phosphate dehydrogenase and the extracellular level of menadione suggested that the enhanced resistance of E. coli 821 to menadione might be due to decreased diaphorase activity, but not to a lowered rate of menadione uptake.
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Perm, 614081, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Smirnova
authors.forename: N G
authors.initials: NG
authors.lastname: Muzyka
authors.forename: M N
authors.initials: MN
authors.lastname: Glukhovchenko
authors.forename: T A
authors.initials: TA
authors.lastname: Krasnykh
authors.forename: O N
authors.initials: ON
authors.lastname: Oktyabrsky
mesh_terms: D002374:Catalase / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D018384:Oxidative Stress; D014812:Vitamin K / Q000494:pharmacology
chemical_list: D014812:Vitamin K; D006861:Hydrogen Peroxide; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D016660:NAD(P)H Dehydrogenase (Quinone); D005978:Glutathione