Differential expression of allograft inflammatory factor 1 and of glutathione peroxidase during auto- and allograft response in marine sponges.
Very recently, Porifera (sponges) have been proven to be suitable model systems to study auto- and allograft recognition at the molecular level. Several potential immune molecules have been isolated from the marine sponges Suberites domuncula and Geodia cydonium, among them those which comprise Ig-like domains in their extracellular part. Here we report on the isolation of two cDNAs from S. domuncula that code for molecules involved in mammals in cytokine-mediated graft response; a putative allograft inflammatory factor 1 (AIF-1) and a non-selenium glutathione peroxidase (GPX). Both polypeptides share high similarity with the corresponding mammalian proteins. The expression of the two genes during auto- and allograft recognition in S. domuncula and G. cydonium was determined. It is shown that the expression of the AIF-1-related gene is upregulated only in allografts, while the GPX-related gene is expressed in the fusion zones formed between auto- as well as allografts. Taken together, these findings suggest that besides cell-mediated defense reactions a cytokine-dependent immune response is also elicited during graft recognition in sponges.
Kruse
M
M
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany.
Steffen
R
R
Batel
R
R
Müller
I M
IM
Müller
W E
WE
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002347:Carps; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000235:genetics*; D006084:Graft Rejection; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D011161:Porifera / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D051381:Rats; D016415:Sequence Alignment; D014182:Transplantation, Autologous / Q000502:physiology*; D014184:Transplantation, Homologous / Q000502:physiology*
C112986:AIF1 protein, human; C491049:Aif1 protein, mouse; C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008840:Microfilament Proteins; D005979:Glutathione Peroxidase