Karyotypes of Saccharomyces sensu lato species.
An improved pulsed-field electrophoresis program was developed to study differently sized chromosomes within the genus Saccharomyces. The number of chromosomes in the type strains was shown to be nine in Saccharomyces castellii and Saccharomyces dairenensis, 12 in Saccharomyces servazzii and Saccharomyces unisporus, 16 in Saccharomyces exiguus and seven in Saccharomyces kluyveri. The sizes of individual chromosomes were resolved and the approximate genome sizes were determined by the addition of individual chromosomes of the karyotypes. Apparently, the genome of S. exiguus, which is the only Saccharomyces sensu lato yeast to contain small chromosomes, is larger than that of Saccharomyces cerevisiae. On the other hand, other species exhibited genome sizes that were 10-25% smaller than that of S. cerevisiae. Well-defined karyotypes represent the basis for future genome mapping and sequencing projects, as well as studies of the origin of the modern genomes.
Petersen
R F
RF
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
Nilsson-Tillgren
T
T
Piskur
J
J
D015825:Chromosomes, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D016681:Genome, Fungal; D007621:Karyotyping; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics*