Identification of proteins that interact with NF-YA.
We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHXI) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.
Yamada
K
K
Department of Biochemistry, Fukui Medical University, Japan. kazuya@fmsrsa.fukui-med.ac.jp
Osawa
H
H
Granner
D K
DK
D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics; D010957:Plasmids; D011485:Protein Binding; D026362:Serum Response Factor; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D015003:Yeasts; D016335:Zinc Fingers
D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C492061:NFYA protein, human; C492060:Nfya protein, mouse; D009687:Nuclear Proteins; D026362:Serum Response Factor; D014157:Transcription Factors; C484404:ZHX1 protein, human