[The immunobiological properties of the lipopolysaccharides of bacteria in the genus Shigella with contrasting virulence and their chemically modified analogs].
The antibody formation and expression of delayed type hypersensitivity (DTH) of several antigens under the conditions of their injection together with bacterial lipopolysaccharide (LPS) in mice have been studied. Water-phenol LPS extract from S. sonnei was used. The LPS was treated with the redox system (LPS-R) or not (LPS-PW). Besides, LPS was obtained by immunoaffinity method from avirulent (LPS-A) or virulent (LPS-V) S. sonnei. It had been found that the mice reacted by more intensive increase antibody formation to LPS or sheep erythrocytes if LPS-R or LPS-V were injected alone or together with erythrocytes. More intensive increase of lymph nodes was shown as well. When specified LPS were injected in mice together with E. coli they induced the immunosuppression of DTH. The LPS-PW and LPS-A caused the most feeble reaction of immune system in mice. It has been suggested that the differences of immune reaction are the reflection of conformation processes in LPS molecules.
Borisova
E V
EV
Kyiv Scientific-Research Institute of Epidemiology and Infectious Diseases, Ministry of Public Health of Ukraine.
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000918:Antibody Specificity / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization / Q000379:methods; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D012764:Shigella sonnei / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence / Q000276:immunology
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides