A genetically engineered cell line resistant to subgroup J avian leukosis virus infection (C/J).
A cell line (DF-1¿J) expressing the envelope protein isolated from the ADOL-Hc1 strain of the avian leukosis virus subgroup J (ALV-J) was used to analyze receptor interference to six different isolates of ALV-J as well as ALV subgroups A-D. The traditional gag-specific enzyme-linked immunosorbent assay (ELISA) as well as flow cytometry was used to evaluate viral infection. The parental cell line (DF-1) was susceptible to all ALV subgroups tested while the DF-1¿J cell line was selectively resistant to the subgroup J isolates. The DF-1¿J cell line was resistant to infection by all six ALV-J isolates as determined using the gag-specific ELISA. There was no interference with the other ALV subgroups (A-D) induced by the expression of the ADOL-Hcl envelope. The ALV-J isolates used in this analysis are serologically distinct when analyzed by flow cytometry. Convalescent sera to ADOL-Hcl cross-reacts with all of the ALV-J isolates tested; however, sera to HPRS-103 did not bind to four of the six isolates. Based on the intensity and differential binding of these antisera using flow cytometry, the six ALV-J isolates used can be grouped into four categories. Thus the DF-1¿J cell line is resistant to infection by a serologically and genetically diverse group of ALV-J isolates and should be useful as a diagnostic tool.
Hunt
H D
HD
Avian Disease and Oncology Laboratory, Agricultural Research Service, United States Department of Agriculture, 3606 E. Mt. Hope Road, East Lansing, Michigan 48823, USA. hunthd@pilot.msu.edu
Lee
L F
LF
Foster
D
D
Silva
R F
RF
Fadly
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D001354:Avian Leukosis Virus / Q000472:pathogenicity* / Q000502:physiology*; D002460:Cell Line; D002642:Chick Embryo; D003429:Cross Reactions; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D005434:Flow Cytometry; D015750:Genes, gag; D005818:Genetic Engineering; D007106:Immune Sera; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011991:Receptors, Virus / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D007106:Immune Sera; D021382:Protein Sorting Signals; D011991:Receptors, Virus; D014759:Viral Envelope Proteins