The induction of cyclooxygenase-2 (COX-2) in cultured endothelial cells treated with serum from preeclampsia is mediated by interleukin-6.
COX-2 protein, but not COX-1 protein, was induced in HUVEC from women with a normal pregnancy (nHUVEC) treated with serum from patients with preeclampsia (pSerum), but not with serum from women with a normal pregnancy (nSerum). COX activity in pSerum treated nHUVEC was less than in nSerum treated nHUVEC. Interestingly, the induction of COX-2 protein in nHUVEC treated with pSerum was inhibited by antiIL-6 antibody. The decreased COX activity in nHUVEC treated with pSerum plus antiIL-6 antibody was also reversed in a dose dependent manner. Thus, the induction of COX-2 in pSerum treated nHUVEC was mediated by IL-6. Therefore, the development of selective inhibitors of COX-2 or of IL-6 antagonists may have a potential role in the prevention and treatment of preeclampsia.
Akarasereenont
P
P
Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Techatrisak
K
K
Chotewuttakorn
S
S
Thaworn
A
A
D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D004727:Endothelium / Q000166:cytology*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D007527:Isoenzymes / Q000097:blood*; D008565:Membrane Proteins; D010544:Peroxidases / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood* / Q000503:physiopathology*; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood*
D015850:Interleukin-6; D007527:Isoenzymes; D008565:Membrane Proteins; D010544:Peroxidases; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases