title: The effect of Lazaroid U-74389G on extended liver resection with ischemia in dogs.
abstract: In liver surgery, total clamping of the portal triad (Pringle's procedure) is commonly used, and it sometimes causes liver failure. This study was designed to evaluate the effect of Lazaroid U-74389G (LAZ-G), which inhibits iron-dependent lipid peroxidation, on ischemia-reperfusion injury during liver resection in dogs.
  
  
  
  The experiment animals were divided into 2 groups. The control group was subjected to 60 minutes of warm ischemia by partial inflow occlusion. The LAZ-G-treated group received LAZ-G before ischemia and then underwent liver ischemia. After reperfusion, the nonischemic lobes were resected, and the remnant liver function was evaluated.
  
  
  
  The LAZ-G-treated group showed a significantly improved animal survival rate. Biochemical analysis and morphologic evaluation by electron microscopy suggest that LAZ-G pretreatment protects both hepatic parenchymal cells and sinusoidal endothelial cells from ischemia-reperfusion injury. Expression of IL-1 beta messenger RNA in hepatic venous blood was measured by a reverse transcriptase-polymerase chain reaction; it was shown to be inhibited in the LAZ-G-treated group after reperfusion. This suggests that LAZ-G decreases the activation of proinflammatory cytokine expression.
  
  
  
  Lazaroid U-74389G ameliorates ischemia-reperfusion injury caused by Pringle's procedure during extensive liver resection. This agent may therefore be clinically applicable for extended liver surgery involving vascular isolation.
authors.affiliation: Second Department of Surgery, Gunma University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Iwanami
authors.forename: I
authors.initials: I
authors.lastname: Takeyoshi
authors.forename: S
authors.initials: S
authors.lastname: Ohwada
authors.forename: J
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawashima
authors.forename: M
authors.initials: M
authors.lastname: Aiba
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Morishita
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods; D006503:Hepatic Veins; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007375:Interleukin-1 / Q000235:genetics; D007511:Ischemia / Q000517:prevention & control*; D008099:Liver / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D017093:Liver Failure / Q000517:prevention & control; D008297:Male; D011281:Pregnatrienes / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000097:blood; D015427:Reperfusion Injury / Q000517:prevention & control
chemical_list: D007166:Immunosuppressive Agents; D007375:Interleukin-1; D011281:Pregnatrienes; D012333:RNA, Messenger; C074965:U 74389F