title: Migration of neutrophils is dependent on mast cells in nonspecific pleurisy in rats.
abstract: To determine the role of mast cells in the recruitment of neutrophils and eosinophils, acute nonspecific pleurisy was induced by injecting isologous serum into normal +/+ and mast cell-deficient Ws/Ws rats. In +/+ rats, neutrophil infiltration peaked 4 h after serum administration, followed by influx of eosinophils after 24-48 h. The levels of neutrophil influx after 4 h as well as the activity of myeloperoxidase (MPO) in pleural lavage-cell extract were significantly lower in Ws/Ws rats than in +/+ rats. In contrast, numbers of eosinophils as well as activity of eosinophil peroxidase (EPO) did not differ significantly between Ws/Ws and +/+ rats. For local reconstitution of mast cells, +/+ rat peritoneal mast cells (PMC) or mesenteric lymph node cells (MLNC) as a control were transferred into the Ws/ Ws pleural cavity. Serum injection into animals with PMC transfer 7 days previously triggered augmented neutrophil influx by approximately 4.7-fold as compared to that in MLNC-transferred animals. Mast cells recovered from the pleural cavity of PMC-transferred rats showed histamine contents equivalent to 20% of that of freshly isolated PMC and retained the reactivity to compound 48/80. These results indicated that dependency of neutrophil recruitment on resident mast cells is greater than that of eosinophils in isologous serum-induced pleurisy.
authors.affiliation: Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: R
authors.initials: R
authors.lastname: Uchikawa
authors.forename: T
authors.initials: T
authors.lastname: Tegoshi
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Hyoh
authors.forename: N
authors.initials: N
authors.lastname: Arizono
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D017690:Cell Transplantation; D002634:Chemotaxis, Leukocyte; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000201:enzymology / Q000276:immunology; D005260:Female; D006636:Histamine Release; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000502:physiology* / Q000637:transplantation; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D010994:Pleura; D010998:Pleurisy / Q000276:immunology*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains
chemical_list: D047209:Eosinophil Peroxidase; D010544:Peroxidases; D009195:Peroxidase; D019009:Proto-Oncogene Proteins c-kit