Detection of casein fragments in an invertebrate and in a vertebrate using in situ hybridization.
Using a non-radioactive in situ hybridization procedure it has been demonstrated that both invertebrates such as the mollusc Mytilus galloprovincialis and lower vertebrates such as the fish Cyprinus carpio express bovine alphaS1- and kappa-casein homologous mRNAs. In particular, positive results were found in molluscan immunocytes, and in cells located in different fish tissues: intestine, endocrine pancreas and kidney. These findings suggest that the casein genes are highly conserved throughout evolution.
Ottaviani
E
E
Department of Animal Biology, Modena, Italy. ottaviani.enzo@unimo.it
Franchini
A
A
Prinzenberg
E M
EM
Erhardt
G
G
Jollès
P
P
D000818:Animals; D049872:Bivalvia; D002347:Carps; D002364:Caseins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D002417:Cattle; D017124:Conserved Sequence; D019143:Evolution, Molecular; D015870:Gene Expression; D017403:In Situ Hybridization; D009928:Organ Specificity; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D002364:Caseins; D010446:Peptide Fragments; D012333:RNA, Messenger