Salivary immunoglobulin A production against a synthetic oligopeptide antigen of Actinobacillus actinomycetemcomitans fimbriae.
Actinobacillus actinomycetemcomitans is an important pathogen in human periodontal diseases. We attempted to produce effective antibodies against A. actinomycetemcomitans fimbrial antigen, which was shown to be an attachment factor. An oligopeptide based on the amino acid sequence of A. actinomycetemcomitans fimbriae was synthesized and conjugated with branched lysine polymer resin beads. Mice were immunized with the synthetic antigen together with one or more of Freund's incomplete adjuvant, the Ribi adjuvant system, liposomes, mouse interleukin 4 expression plasmids, and cholera toxin. After immunization, salivary immunoglobulin A (IgA) and serum IgG levels against the synthetic oligopeptide antigen were determined by enzyme-linked immunosorbent assay. The serum IgG responses against the fimbrial antigen were significantly higher in animals immunized intramuscularly with the Ribi adjuvant system. A high salivary IgA response to the fimbriae was induced by intranasal mucosal immunization with cholera toxin and mouse interleukin 4 expression plasmids.
Honma
K
K
Oral Health Science Center, Tokyo Dental College, Japan.
Kato
T
T
Okuda
K
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D017358:Cell Wall Skeleton / Q000494:pharmacology; D003311:Cord Factors / Q000494:pharmacology; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D008050:Lipid A / Q000031:analogs & derivatives / Q000494:pharmacology; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000138:chemical synthesis; D012463:Saliva / Q000276:immunology; D014614:Vaccines, Synthetic
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D017358:Cell Wall Skeleton; D003311:Cord Factors; D007071:Immunoglobulin A, Secretory; D008050:Lipid A; D008081:Liposomes; D009842:Oligopeptides; C075897:Ribi adjuvant; D014614:Vaccines, Synthetic