title: [Use of a rapid detection assay for influenza virus, on nasal aspirate specimens].
abstract: We investigated the usefulness of a rapid antigen detection kit using optical immunoassay for influenza virus (FLU OIA, BioStar, USA). Nasal aspirates were taken from 92 influenza suspected outpatients between March to April of 1999. Compared with virus isolation and PCR, the sensitivity of FLU OIA was 88.5% and 81.6%, and the specificity was 65.2% and 72.2%. All isolated viruses were influenza type B virus. It was difficult to differentiate the weak-positive and negative cases, leading to the rather low specificity, although the assay procedure was easy and quick. FLU OIA may be a useful rapid diagnosis kit for influenza in pediatric outpatient clinics and wards, because it can detect both influenza type A and type B viruses.
authors.affiliation: Department of Pediatrics, Isehara Kyodo Hospital.
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: O
authors.initials: O
authors.lastname: Komiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Mishiku
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Sugaya
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: N
authors.initials: N
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Maezawa
authors.forename: M
authors.initials: M
authors.lastname: Imai
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D009981:Influenza B virus / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis*; D009093:Mucus / Q000821:virology*; D009297:Nasal Mucosa / Q000821:virology*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic