Effect of dental materials on gluconeogenesis in rat kidney tubules.
The effect of dental composite components triethyleneglycoldimethacrylate (TEGDMA) and hydroxyethylmethacrylate (HEMA) as well as mercuric chloride (HgCl(2)) and methylmercury chloride (MeHgCl) on gluconeogenesis was investigated in isolated rat kidney tubules. From starved rats kidney tubules were prepared and isolated by digestion with collagenase. Every 10 min up to 60 min 1-ml samples were drawn from the cell suspension for quantitating the glucose content. Glucose formation in controls was 3.3 +/- 0.2 nmol/mg. per min (mean +/- SEM, n=21). Relative rates of glucose formation were obtained by expressing individual rates as a percentage of the corresponding control. X-Y concentration curves (effective concentration, EC) of the substances were calculated by fitting a four-parametric sigmoid function to the relative rates of glucose formation at various test concentrations. At the end of the incubation period cell viability was assessed by trypan blue exclusion. Cell viability decreased within the 60 min interval from 90 to approx. 80% (controls), <25 (HEMA), <20 (TEGDMA), <10 (MeHgCl), and <10% (HgCl(2)). Values of 50% effective concentration (EC(50)) were calculated from fitted curves. EC(50) values were (mmol; mean +/- SEM; n=4): HEMA, 17.7 +/- 2.9; TEGDMA, 1.8 +/- 0.2; MeHgCl, 0.018 +/- 0.0005; and HgCl(2), 0. 0016 +/- 0.0005. The toxic effect of HgCl(2) was approximately 1000 or 10 000 higher than that of the dental composite components TEGDMA or HEMA, respectively.
Reichl
F X
FX
Walther-Straub-Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University of Munich, Nussbaumstrasse 26, D-80336 Munich, Germany. F.X.Reichl@lrz.uni-muenchen.de
Durner
J
J
Mückter
H
H
Elsenhans
B
B
Forth
W
W
Kunzelmann
K H
KH
Hickel
R
R
Spahl
W
W
Hume
W R
WR
Moes
G W
GW
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003188:Composite Resins / Q000633:toxicity*; D005943:Gluconeogenesis / Q000187:drug effects*; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity*; D008767:Methylmercury Compounds / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003188:Composite Resins; D008689:Methacrylates; D008767:Methylmercury Compounds; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D008627:Mercuric Chloride; C005044:hydroxyethyl methacrylate; C004925:methylmercuric chloride