A novel calcitonin receptor gene in human osteoclasts from normal bone marrow.
The calcitonin receptor (CTR) gene in human osteoclasts formed in a human bone marrow cell culture system was examined by reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR results indicated that the 5'-untranslated region (5'UTR) was different between CTR mRNAs in human osteoclasts and in a mammary tumor cell line, MCF-7 cells. We isolated the 5'UTR of the CTR gene from human osteoclasts, whose sequence had only 28.6% identity with that of other CTR genes reported until now. In a radioligand binding assay, COS-1 cells transfected with the osteoclast CTR gene bound to [125I]human CT (hCT). These results provided evidence that the CTR gene cloned from human osteoclasts was expressed functionally and its coding protein was identical to MCF-7 cell CTR.
Nishikawa
T
T
Department of Orthopaedic Surgery, Tokyo Metropolitan Geriatric Hospital, Japan.
Ishikawa
H
H
Yamamoto
S
S
Koshihara
Y
Y
D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism*; D001943:Breast Neoplasms; D019556:COS Cells; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010010:Osteoclasts / Q000378:metabolism*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018003:Receptors, Calcitonin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014162:Transfection; D014407:Tumor Cells, Cultured
D020121:5' Untranslated Regions; D012333:RNA, Messenger; D018003:Receptors, Calcitonin