Irradiation-mediated rescue of T cell-specific V(D)J recombination and thymocyte differentiation in severe combined immunodeficient mice by bone marrow cells.
In SCID (severe combined immunodeficient) mice, proper assembly of immunoglobulin and T cell receptor (TCR) genes is blocked by defective V(D)J recombination so that B and T lymphocyte differentiation is arrested at an early precursor stage. Treating the mice with gamma irradiation rescues V(D)J rearrangement at multiple TCR loci, promotes limited thymocyte differentiation, and induces thymic lymphomas. These effects are not observed in the B cell lineage. Current models postulate that irradiation affects intrathymic T cell precursors. Surprisingly, we found that transfer of irradiated SCID bone marrow cells to unirradiated host animals rescues both TCR rearrangements and thymocyte differentiation. These data indicate that irradiation affects precursor cells at an earlier stage of differentiation than was previously thought and suggest new models for the mechanism of irradiation rescue.
Wang
C
C
Department of Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA.
Bogue
M A
MA
Levitt
J M
JM
Roth
D B
DB
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000528:radiation effects*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation / Q000528:radiation effects; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D005434:Flow Cytometry; D005720:Gamma Rays; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology / Q000528:radiation effects*; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000528:radiation effects*; D011995:Recombination, Genetic / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology / Q000528:radiation effects
D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; D011948:Receptors, Antigen, T-Cell; C064655:V(D)J recombination activating protein 2