title: Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A.
abstract: Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2-cyclin A activity.
authors.affiliation: Department of Molecular Biology, University of Geneva, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Meraldi
authors.forename: J
authors.initials: J
authors.lastname: Lukas
authors.forename: A M
authors.initials: AM
authors.lastname: Fry
authors.forename: J
authors.initials: J
authors.lastname: Bartek
authors.forename: E A
authors.initials: EA
authors.lastname: Nigg
mesh_terms: D000818:Animals; D042846:CDC2-CDC28 Kinases; D016466:CHO Cells; D002352:Carrier Proteins; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D018385:Centrosome / Q000502:physiology* / Q000648:ultrastructure*; D006224:Cricetinae; D019925:Cyclin A / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D004261:DNA Replication; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D008322:Mammals; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D056465:Retinoblastoma-Binding Protein 1; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D019925:Cyclin A; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D011994:Recombinant Proteins; D056465:Retinoblastoma-Binding Protein 1; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases