Synthesis and fluorescence properties of Oregon Green 514 labeled peptides.
Oregon Green 514, with high photostability and a pKa of 4.8, is suitable for imaging applications. Labeling of peptide-bound resin with the carboxylic acid form was optimized. Peptide conjugation resulted in altered fluorescence properties of the dye including a quenching of the intensity compared with that in aqueous buffers with a slight red-shift of the emission maximum.
Delmotte
C
C
Centre de Biophysique Moléculaire, UPR 4301 - CNRS affiliée à I'université, Orléans, France.
Delmas
A
A
D002021:Buffers; D004563:Electrochemistry; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D017830:Octoxynol; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002021:Buffers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008823:Micelles; D010455:Peptides; C401939:oregon green 514; D017830:Octoxynol