Characterization of a subfamily of related winged helix genes, XFD-12/12'/12" (XFLIP), during Xenopus embryogenesis.
The fork head domain family of genes defines a growing group of proteins that serve important regulatory functions in pattern-forming events of both invertebrates and vertebrates. Here we add three closely related, novel members to this family in Xenopus laevis, termed XFD-12, XFD-12' and XFD-12". All three genes reveal indistinguishable expression patterns during Xenopus embryogenesis. During gastrulation, XFD-12 type transcripts are detected exclusively in the superficial layer of cells within the Spemann organizer territory. In the open neural plate, XFD-12 type expression defines a row of cells located along the dorsal midline and destined to become the floor plate of the neural tube. After closure of the neural tube, XFD-12 type encoding mRNAs are only detected in the tailtip and a small area located at the midbrain/hindbrain boundary. Within the Spemann organizer and in the floor plate area, expression of XFD-12 type genes is only partially overlapping with XFD-1 expression.
Sölter
M
M
Abteilung Biochemie, Universität Ulm, Albert Einstein Allee 11, D-89081, Ulm, Germany.
Köster
M
M
Hollemann
T
T
Brey
A
A
Pieler
T
T
Knöchel
W
W
D000595:Amino Acid Sequence; D000818:Animals; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D004475:Ectoderm; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000528:radiation effects; D051858:Forkhead Transcription Factors; D005775:Gastrula; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D018021:Lithium Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014466:Ultraviolet Rays; D014981:Xenopus / Q000196:embryology*; D029867:Xenopus Proteins
D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D051858:Forkhead Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D029867:Xenopus Proteins; C525812:bmp4 protein, Xenopus; D018021:Lithium Chloride