Molecular cloning of cDNA encoding a cyclin-selective ubiquitin carrier protein (E2-C) from Carassius auratus (goldfish) and expression analysis of the cloned gene.
Destruction of cyclin B is required for exit from mitosis and meiosis. A cyclin-specific ubiquitinating system, including cyclin-selective ubiquitin carrier protein (E2-C), is thought to be responsible for cyclin B destruction. Here we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, E2-C which encodes the cyclin-selective ubiquitin carrier protein from goldfish ovary. The cloned cDNA is 677 bp long and encodes 172 amino acids. The deduced amino acid sequence is highly homologous to E2-C from other species. Recombinant goldfish E2-C possesses ubiquitinating activity against cyclin B. The expression of mRNA for E2-C was similar to that of mRNA for cyclin B, occurring at very high level in the ovary. The similarity of the expression pattern of E2-C and cyclin B suggests that E2-C mediates a cyclin-specific ubiquitination.
Tokumoto
M
M
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
Nagahama
Y
Y
Tokumoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli; D005786:Gene Expression Regulation; D006054:Goldfish / Q000235:genetics*; D008025:Ligases; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D044763:Ubiquitin-Conjugating Enzymes
D002352:Carrier Proteins; D019926:Cyclin B; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; C046288:ubiquitin carrier proteins; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases