title: In vivo testing of the protective efficacy of gloves against allergen-containing products using an open chamber system.
abstract: In vitro degradation and permeation testing of glove materials is important in the assessment of the protective efficacy against chemicals. In vivo factors, such as skin-glove contact, skin temperature and humidity may, however, influence the protective effect of the glove. These factors must thus be considered in the overall assessment of a protective glove. An in vivo glove test should as far as possible imitate the practical use of the glove, as well as the exposure to the product/chemical against which the glove should protect. An open chamber system for human in vivo glove testing is presented. This system enables simultaneous testing of 6-8 gloves with 3 provocation times for each glove. Positive controls (no glove) can be included. As a control of the subject's present reactivity to the chemical of interest, conventional patch testing with a dilution series can be performed in parallel. The method is easy to use and convenient for the patient. It promises to be a useful clinical tool in individual preventive measures against contact allergy. The method can be used in glove testing against many hazardous chemicals, both contact allergens and toxic/irritant compounds in workplaces such as the plastics industry and the chemical industry.
authors.affiliation: Department of Biomedicine and Surgery, University Hospital, Linköping, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Andersson
authors.forename: M
authors.initials: M
authors.lastname: Bruze
mesh_terms: D000485:Allergens; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis / Q000517:prevention & control*; D009783:Dermatitis, Occupational / Q000175:diagnosis / Q000517:prevention & control*; D017575:Gloves, Protective; D006801:Humans
chemical_list: D000485:Allergens