title: Differential effect of estradiol on antibody secretion of murine hybridomas.
abstract: The need for increased antibody production by hybridomas has been approached by the addition to cell cultures of different growth factors; in vitro addition of estradiol-17beta (E2) to human blood lymphocytes increases the accumulation of plasma-blasts and Ig-secreting cells. Four different murine-murine hybridomas secreting different monoclonal antibodies (MAbs) were treated with E2. Specific antibody concentration was measured by enzyme-linked immunoadsorbent assay (ELISA) in culture supernatants whereas expression of E2-receptor in the hybridoma cells was determined by polymerase chain reaction (PCR). When E2 was added as a growth supplement to alpha-estrogen receptor positive murine-murine hybridomas it enhanced MAb secretion by as much as 255%, in a dose-dependant manner. This effect lasted for as long as the alpha-estrogen receptor was detected in the hybridoma cells, was inhibited by tamoxifen and was not observed in alpha-estrogen receptor negative hybridomas. The synthetic estrogen analogue diethylstilbestrol had no effect. Estradiol-17beta should be added to the list of hybridoma-inducing growth factors.
authors.affiliation: Department de Biología Celular, Instituto Nacional de Cardiología Ignacio Chávez, México, DF Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Molina
authors.forename: F
authors.initials: F
authors.lastname: Massó
authors.forename: A
authors.initials: A
authors.lastname: Paez
authors.forename: C
authors.initials: C
authors.lastname: Mendez
authors.forename: E
authors.initials: E
authors.lastname: Rodríguez
authors.forename: J J
authors.initials: JJ
authors.lastname: Mandoki
authors.forename: L
authors.initials: L
authors.lastname: Díaz de Léon
authors.forename: L F
authors.initials: LF
authors.lastname: Montaño
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000921:Antibody-Producing Cells / Q000737:chemistry; D001402:B-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D004054:Diethylstilbestrol / Q000276:immunology / Q000494:pharmacology; D004958:Estradiol / Q000276:immunology* / Q000494:pharmacology*; D005260:Female; D006825:Hybridomas / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology*; D007074:Immunoglobulin G / Q000187:drug effects; D008297:Male; D051379:Mice; D011960:Receptors, Estrogen / Q000032:analysis / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D011960:Receptors, Estrogen; D004958:Estradiol; D004054:Diethylstilbestrol