HemT-3, an alternative transcript of mouse gene HemT specific to male germ cells.
A number of genes are known to be expressed primarily in hematopoietic cells and testis and are thought to function in the control of both blood cell and male germ cell differentiation. We have recently identified a mouse gene, HemT, that encodes two alternatively spliced transcripts specific to hematopoietic cells (HemT-1 and HemT-2) and kidney (HemT-2). We have now isolated a third HemT transcript, HemT-3, that is found only in testis by Northern blot analysis and RT-PCR. HemT-3 is alternatively spliced and may be initiated differently from HemT-1 and HemT-2. RNA in-situ hybridization of testis from wild-type and germ-cell-deficient adult mice, as well as from mice at different developmental stages, indicates that HemT-3 is expressed only in early spermatocytes. HemT-3 cDNA has a major open reading frame related to a human glycosylphosphatidylinositol (GPI)-anchored protein, GML. Using an antibody generated against a peptide derived from the HemT-3 open reading frame, we have detected a testis-specific 22kDa protein by Western blot analysis.
Xue
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Department of Genetics, Columbia University College of Physicians and Surgeons, New York, NY, USA.
O'Neill
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D
Wang
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Wolgemuth
D J
DJ
Bank
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A
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D020033:Protein Isoforms / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000378:metabolism*; D013737:Testis / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic
D001798:Blood Proteins; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C102519:GML protein, human; D058851:GPI-Linked Proteins; C119617:HemT protein, mouse; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D012313:RNA