MPS1: a small, evolutionarily conserved zinc finger protein from the protozoan Toxoplasma gondii.
Within the expressed sequence tag (EST) dataset of Toxoplasma gondii we have identified several ESTs encoding a protein similar to the small zinc finger protein MPS1. In human it is suggested that MPS1 plays a role as a transcriptional mediator in response to various growth factors and it is used as a tumour marker in sera from cancer patients. However, in rat a cDNA sequence homologous to MPS1 encodes ribosomal protein S27. To further characterise MPS1 in T. gondii we transformed tachyzoites with a c-Myc-tagged version of the Toxoplasma MPS1 cDNA, flanked by SAG1 sequences. Western blot analysis showed that the Myc-MPS1 was only poorly expressed in the stable transformants. In contrast, Northern blot analysis demonstrated that the Myc-MPS1 mRNA was abundantly transcribed and that the endogenous level of MPS1 mRNA was not affected.
Mattsson
J G
JG
ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D-69120, Heidelberg, Germany. jens.mattsson@sva.se
Soldati
D
D
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics; D016415:Sequence Alignment; D014122:Toxoplasma / Q000235:genetics* / Q000254:growth & development; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D008667:Metalloproteins; D009687:Nuclear Proteins; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C489993:Rps27 protein, rat