Acyl-coenzyme A binding protein (ACBP).
Acyl-coenzyme A binding proteins are known from a large group of eukaryote species and to bind a long chain length acyl-CoA ester with very high affinity. Detailed biochemical mapping of ligand binding properties has been obtained as well as in-depth structural studies on the bovine apo-protein and of the complex with palmitoyl-CoA using NMR spectroscopy. In the four alpha-helix bundle structure, a set of 21 highly conserved residues present in more that 90% of all known sequences of acyl-coenzyme A binding proteins constitutes three separate mini-cores. These residues are predominantly located at the helix-helix interfaces. From studies of a large set of mutant proteins the role of the conserved residues has been related to structure, function, folding and stability.
Kragelund
B B
BB
Carlsberg Laboratory, Department of Chemistry, Gl. Carlsberg Vej 10, DK-2500, Valby, Denmark.
Knudsen
J
J
Poulsen
F M
FM
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D026261:Diazepam Binding Inhibitor; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016296:Mutagenesis; D016415:Sequence Alignment
D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor