Nucleosome positioning is independent of histone H1 in vivo.
Nucleosome positioning in the somatic macronuclear genome of the ciliated protozoan Tetrahymena thermophila was analyzed by indirect end labeling. Nucleosomes were positioned nonrandomly in three different regions of the Tetrahymena genome. Nucleosome repeat length varied between adjacent nucleosomes. Nucleosome positioning in a histone H1 knockout strain was indistinguishable from that in a strain with wild type histone H1.
Karrer
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KM
Department of Biology, Marquette University, Milwaukee, Wisconsin 53201-1881, USA. kathleen,karrer@marquette.edu
VanNuland
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TA
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics; D002843:Chromatin / Q000378:metabolism; D018390:Gene Targeting; D006657:Histones / Q000172:deficiency / Q000235:genetics*; D009707:Nucleosomes / Q000378:metabolism*; D015183:Restriction Mapping; D016808:Tetrahymena thermophila / Q000235:genetics*
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes