title: Characteristics of sheep-rumen isolates of Pseudomonas aeruginosa inhibitory to the growth of Escherichia coli O157.
abstract: Screening facultative sheep-rumen bacteria which inhibit growth of Escherichia coli produced 11 strains of Pseudomonas aeruginosa. The isolates showed three different pulsed-field gel electrophoresis patterns and strains from different sheep produced pyocins that varied in strain specificity. Representative strains were resistant to ampicillin, methicillin, erythromycin, fusidic acid and augmentin, but not to tetracycline or nalidixic acid. Tested strains attached in large numbers to cultured rumen epithelial cells, potentially providing a means of survival in this ecosystem.
authors.affiliation: Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK. shd@rri.sari.ac.uk
authors.forename: S H
authors.initials: SH
authors.lastname: Duncan
authors.forename: C J
authors.initials: CJ
authors.lastname: Doherty
authors.forename: J R
authors.initials: JR
authors.lastname: Govan
authors.forename: S
authors.initials: S
authors.lastname: Neogrady
authors.forename: P
authors.initials: P
authors.lastname: Galfi
authors.forename: C S
authors.initials: CS
authors.lastname: Stewart
mesh_terms: D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000254:growth & development*; D005227:Fatty Acids / Q000032:analysis; D010860:Pigments, Biological / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D011709:Pyocins / Q000096:biosynthesis / Q000145:classification; D012417:Rumen / Q000382:microbiology*; D012756:Sheep / Q000382:microbiology*
chemical_list: D005227:Fatty Acids; D010860:Pigments, Biological; D011709:Pyocins