title: Hedgehog movement is regulated through tout velu-dependent synthesis of a heparan sulfate proteoglycan.
abstract: Hedgehog (Hh) molecules play critical roles during development as a morphogen, and therefore their distribution must be regulated. Hh proteins undergo several modifications that tether them to the membrane. We have previously identified tout velu (ttv), a homolog of the mammalian EXT tumor suppressor gene family, as a gene required for movement of Hh. In this paper, we present in vivo evidence that ttv is involved in heparan sulfate proteoglycan (HSPG) biosynthesis, suggesting that HSPGs control Hh distribution. In contrast to mutants in other HSPG biosynthesis genes, the activity of the HSPG-dependent FGF and Wingless signaling pathways are not affected in ttv mutants. This demonstrates an unexpected level of specificity in the regulation of the distribution of extracellular signals by HSPGs.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: I
authors.initials: I
authors.lastname: The
authors.forename: Y
authors.initials: Y
authors.lastname: Bellaiche
authors.forename: N
authors.initials: N
authors.lastname: Perrimon
mesh_terms: D000818:Animals; D015415:Biomarkers; D004330:Drosophila / Q000196:embryology; D029721:Drosophila Proteins; D005346:Fibroblast Growth Factors / Q000378:metabolism; D005786:Gene Expression Regulation; D053823:Hedgehog Proteins; D019812:Heparan Sulfate Proteoglycans / Q000096:biosynthesis* / Q000378:metabolism; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D015398:Signal Transduction; D051155:Wnt1 Protein
chemical_list: D015415:Biomarkers; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D019812:Heparan Sulfate Proteoglycans; D019476:Insect Proteins; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; C113073:TTV protein, Drosophila; D051155:Wnt1 Protein; C074622:wg protein, Drosophila; C077245:hh protein, Drosophila; D005346:Fibroblast Growth Factors