title: The first triple gene block protein of peanut clump virus localizes to the plasmodesmata during virus infection.
abstract: The subcellular localization of the first triple gene block protein (TGBp1) of peanut clump pecluvirus (PCV) was studied by subcellular fractionation and immunogold cytochemistry using TGBp1-specific antibodies raised against a fusion protein expressed in and purified from bacteria. In the inoculated and apical leaves of virus-infected Nicotiana benthamiana, TGBp1 localized to the cell wall and P30 fractions. Electron microscopy of immunogold-decorated ultrathin sections of the infected leaf tissue revealed TGBp1-specific labeling of the plasmodesmata joining mesophyll cells. In longitudinal sections of the plasmodesmata, the TGBp1-specific labeling was most commonly associated with the plasmodesmal collar region. In transgenic N. benthamiana, which constitutively expressed TGBp1, no TGBp1-specific immunogold labeling of plasmodesmata was observed, but plasmodesmata were gold decorated when the transgenic plants were infected with a TGBp1-defective PCV mutant, indicating that factors induced by the virus infection target and/or anchor the transgene TGBp1 to the plasmodesmata.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, et de, l'Université Louis Pasteur, 12 rue du Général Zimmer, Strasbourg Cedex, 67084, France.
authors.forename: M
authors.initials: M
authors.lastname: Erhardt
authors.forename: C
authors.initials: C
authors.lastname: Stussi-Garaud
authors.forename: H
authors.initials: H
authors.lastname: Guilley
authors.forename: K E
authors.initials: KE
authors.lastname: Richards
authors.forename: G
authors.initials: G
authors.lastname: Jonard
authors.forename: S
authors.initials: S
authors.lastname: Bouzoubaa
mesh_terms: D010367:Arachis / Q000821:virology*; D001483:Base Sequence; D017931:DNA Primers; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D013347:Subcellular Fractions / Q000821:virology; D014026:Nicotiana / Q000821:virology
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins