title: Development and evaluation of a rapid dipstick assay for serodiagnosis of acute human brucellosis.
abstract: A dipstick assay for the detection of brucella-specific immunoglobulin M antibodies was evaluated with 707 sera from 247 laboratory-confirmed brucellosis patients and 342 control sera from brucellosis-free individuals. These sera were collected from six different countries. The assay was found to be highly sensitive and specific. In addition, the test is easy to use and does not require specialized training or equipment, and the components are stable without a requirement for refrigeration. All of these factors make the test ideal for developing countries and rural settings.
authors.affiliation: Department of Biomedical Research, Royal Tropical Institute, The Netherlands. H.Smits@kit.nl
authors.forename: H L
authors.initials: HL
authors.lastname: Smits
authors.forename: M A
authors.initials: MA
authors.lastname: Basahi
authors.forename: R
authors.initials: R
authors.lastname: Díaz
authors.forename: T
authors.initials: T
authors.lastname: Marrodan
authors.forename: J T
authors.initials: JT
authors.lastname: Douglas
authors.forename: A
authors.initials: A
authors.lastname: Rocha
authors.forename: J
authors.initials: J
authors.lastname: Veerman
authors.forename: M M
authors.initials: MM
authors.lastname: Zheludkov
authors.forename: O W
authors.initials: OW
authors.lastname: Witte
authors.forename: J
authors.initials: J
authors.lastname: de Jong
authors.forename: G C
authors.initials: GC
authors.lastname: Gussenhoven
authors.forename: M G
authors.initials: MG
authors.lastname: Goris
authors.forename: M A
authors.initials: MA
authors.lastname: van Der Hoorn
mesh_terms: D000208:Acute Disease; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology; D005069:Evaluation Studies as Topic; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M; D011934:Reagent Strips