An improved method for STR analysis of bloodstained denim.
Indigo dye is used to dye denim and other fabrics. It is now accepted that if this is co-extracted with the DNA, it may inhibit PCR amplification. A simple, improved method is described for the extraction of DNA from bloodstained denim for PCR amplification and short tandem repeat (STR) analysis. The DNA was extracted by constructing a blotting system using capillary action to draw a saline solution through the denim. The transferred material was collected onto nylon membranes and these were processed by chelex extraction. A variety of coloured denim substrates and other heavily dyed fabrics, including case work samples were used. In all cases the DNA was extracted, amplified and typed correctly.
Larkin
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A
Institute of Environmental Science and Research Ltd., ESR-Forensic, Mount Albert Science Centre, Private Bag 92-021, Auckland, New Zealand.
Harbison
S
S
D000483:Alleles; D001801:Blood Stains; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008163:Luminescent Measurements; D016133:Polymerase Chain Reaction / Q000379:methods; D020080:Tandem Repeat Sequences
D004247:DNA