An efficient approach for screening minimal PKS genes from Streptomyces.
Degenerated oligonucleotide primers were designed to amplify fragments of ketosynthase genes from polyketide antibiotics producing Streptomyces spp. and bacterial strains enriched from soil samples. Cell lysates were used as templates in amplification, so time-consuming DNA purification was avoided. A phylogenetic tree constructed from the amino acid sequences of the amplified fragments shows a distribution of spore pigments and antibiotics in separate classes. In addition, several different subgroups form within the antibiotics group. Anthracyclines were divided into separate branches according to the starter unit used in biosynthesis.
Metsä-Ketelä
M
M
University of Turku, Biochemistry, FIN-20014, Turku, Finland.
Salo
V
V
Halo
L
L
Hautala
A
A
Hakala
J
J
Mäntsälä
P
P
Ylihonko
K
K
D000595:Amino Acid Sequence / Q000235:genetics; D000903:Antibiotics, Antineoplastic / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D000903:Antibiotics, Antineoplastic; D017931:DNA Primers; D009097:Multienzyme Complexes