title: [In vitro study on MT2 cell infected by hepatitis C virus].
abstract: OBJECTIVE
  To establish an MT-2 cell model infected by HCV in vitro.
  
  
  METHODS
  Human T-lymphocyte cell line MT-2 was infected with HCV-RNA positive serum and detected for HCV RNA by RT-PCR 1, 4, 7, 10, 14, 18, 21, 24, 28, 31, 35 and 39 days after the infection. PCR products were determined by southern blot. MT-2 cell infected with HCV-RNA negative serum was used as control.
  
  
  RESULTS
  HCV-RNA began to turn positive at 7 days postinoculate (p.i.) till at least 28 days(p.i.). Intracellular negative-stranded HCV-RNA was detected from 10 to 18 days (p.i.). No HCV RNA was detected in the control group.
  
  
  CONCLUSIONS
  HCV may replicate and express in MT-2 cell for a short period.
authors.affiliation: Institute of Infectious Diseases, First Affiliated Hospital, Medical Collage Zhejiang University, Hangzhou.
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Chen
mesh_terms: D000328:Adult; D002460:Cell Line; D005260:Female; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000821:virology*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral