A 22 kDa polyanion inhibits carnitine-dependent fatty acid oxidation in rat liver mitochondria.
The transport of activated fatty acids across the mitochondrial outer membrane has not been fully addressed. A polyanion (M(n)=22 kDa) inhibited the ADP-stimulated carnitine-dependent oxidation of both palmitoyl-CoA and palmitate plus CoA as well as mitochondrial hexokinase binding. In contrast, the oxidation of palmitoylcarnitine plus malate, as well as glutamate oxidation, was essentially unaffected. Mitochondrial carnitine palmitoyltransferase-1 was not inhibited by the polyanion. The data suggest an additional component in carnitine-dependent mitochondrial fatty acid oxidation, possibly porin.
Turkaly
P
P
Department of Veterans Affairs Medical Center, Departments of Nutrition, Medicine and Pharmacology, Medical Research Service (151W), 10701 East Blvd., Case Western Reserve University, Cleveland, OH, USA.
Kerner
J
J
Hoppel
C
C
D000818:Animals; D002331:Carnitine / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D008689:Methacrylates / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D000071228:Polyelectrolytes; D011108:Polymers / Q000138:chemical synthesis / Q000378:metabolism*; D011137:Polystyrenes / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002331:Carnitine; D002334:Carnitine O-Palmitoyltransferase; D005227:Fatty Acids; D006593:Hexokinase; D007473:Ion Channels; D008689:Methacrylates; D000071228:Polyelectrolytes; D011108:Polymers; D011137:Polystyrenes; C400544:styrene-co-maleic acid-co-methacrylate polymer