Oxygen radical production by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils.
Oxidative metabolic burst activity by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils was indirectly evaluated by measuring the oxidation of nonfluorescent intracellular 2',7'-dichlorofluorescein (DCFH) to fluorescent 2',7'-dichlorofluorescein (DCF) with flow cytometry. The results were recorded as mean channel fluorescence intensity. Phorbol myristate acetate at 50 ng/ml was used to activate the cells. Elephant heterophils and bovine neutrophils exhibited similar abilities to oxidize intracellular DCFH to DCF, a fluorescent product. A wide range of mean channel fluorescence intensity (1,803-7,393) was observed among individual elephants, and the range of intensities was similar to that observed in bovine neutrophils, supporting the concept of functional heterogeneity of heterophils among elephants.
Tell
L
L
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.
Kabbur
M B
MB
Smith
W L
WL
Gage
L
L
Cullor
J S
JS
D000818:Animals; D002417:Cattle / Q000097:blood* / Q000378:metabolism; D004606:Elephants / Q000097:blood* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000502:physiology*
D005452:Fluoresceins; D005609:Free Radicals; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; C037631:2',7'-dichlorofluorescein