title: Chlamydia pneumoniae in abdominal aortic aneurysms: abundance of membrane components in the absence of heat shock protein 60 and DNA.
abstract: In this article, we describe the results of a comparative study for the detection of Chlamydia pneumoniae in abdominal aortic aneurysm specimens of 19 patients through the use of immunocytochemistry (ICC), in situ hybridization (ISH), and polymerase chain reaction (PCR), along with the detection of cytomegalovirus (CMV) and herpes simplex virus (HSV) by ICC and PCR. C pneumoniae-specific membrane protein was detected in specimens of all 19 (100%; 95% confidence interval [CI] 82% to 100%) and of 15 (79%; 95% CI 54% to 94%) patients with monoclonal antibodies RR-402 and TT-401, respectively. Chlamydial lipopolysaccharide was detected in specimens of 15 (79%; 95% CI 54% to 94%) patients when the results of 4 different monoclonal antibodies were combined. Surprisingly, chlamydial heat shock protein 60 was not detected in any of the specimens by ICC. Furthermore, C pneumoniae DNA was not detected by ISH when a C pneumoniae major outer membrane protein gene fragment was used as probe, nor was it reproducibly detected by PCR on extracted DNA. These results may be explained either by different kinetics of degradation of the different components of C pneumoniae after infection of the vessel wall or by the involvement of other Chlamydia-like microorganisms. Coexistence of C pneumoniae antigens and HSV antigens but not CMV antigens was observed in specimens from 10 of 18 (56%; 95% CI 31% to 78%) patients by ICC. CMV and HSV DNAs were not detected by PCR. In conclusion, we have demonstrated the presence of antigens of C pneumoniae in the absence of specific DNA in abdominal aortic aneurysms, suggesting persistence of the antigens rather than a persistent infection.
authors.affiliation: Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. Adam.Meijer@rivm.nl
authors.forename: A
authors.initials: A
authors.lastname: Meijer
authors.forename: J A
authors.initials: JA
authors.lastname: van Der Vliet
authors.forename: P J
authors.initials: PJ
authors.lastname: Roholl
authors.forename: S K
authors.initials: SK
authors.lastname: Gielis-Proper
authors.forename: A
authors.initials: A
authors.lastname: de Vries
authors.forename: J M
authors.initials: JM
authors.lastname: Ossewaarde
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal; D001012:Aorta, Abdominal / Q000737:chemistry / Q000382:microbiology / Q000821:virology; D017544:Aortic Aneurysm, Abdominal / Q000382:microbiology* / Q000821:virology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D018834:Chaperonin 60 / Q000032:analysis*; D002690:Chlamydia Infections / Q000175:diagnosis*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004279:DNA, Viral; D007074:Immunoglobulin G; D014764:Viral Proteins