[Capillary electrophoresis: principles and practice in clinical laboratory].
Capillary electrophoresis represents a relatively new analytical technique. This methodology has diversified and given rise to various modes such as capillary zone electrophoresis, capillary gel electrophoresis, micellar electrokinetic capillary chromatography, capillary isoelectric focusing and capillary isotachophoresis. If capillary electrophoresis was first introduced in research laboratories, this technique is now making an entrance to the clinical laboratory. This is due to its rapid and high-efficiency separation power, its potential applications and its possible automation. Thus, capillary electrophoresis represents an attractive alternative to some time-consuming techniques. Thanks to its versatility, the use of capillary electrophoresis has been proposed for the separation and quantification of a wide spectrum of biological components ranging from macromolecules (proteins, lipoproteins, nucleic acids) to small analytes (amino acids, organic acids or drugs). This paper illustrates the potential of capillary electrophoresis which should rapidly become a major technology for a modern clinical laboratory.
Blessum
C
C
Service d'immunologie, Centre hospitalier Lyon-Sud, 69495 Pierre-Bénite, France.
Jeppsson
J O
JO
Aguzzi
F
F
Bernon
H
H
Bienvenu
J
J
D000435:Alcoholic Intoxication / Q000175:diagnosis; D000596:Amino Acids / Q000032:analysis; D001536:Bence Jones Protein / Q000652:urine; D001798:Blood Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005227:Fatty Acids / Q000032:analysis; D006453:Hemoglobinopathies / Q000175:diagnosis; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007525:Isoelectric Focusing / Q000379:methods; D008074:Lipoproteins / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis; D011041:Poisoning / Q000175:diagnosis; D011507:Proteinuria / Q000175:diagnosis; D014168:Transferrin / Q000032:analysis
D000596:Amino Acids; D001798:Blood Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D006454:Hemoglobins; D007136:Immunoglobulins; D008074:Lipoproteins; D009696:Nucleic Acids; D004364:Pharmaceutical Preparations; D014168:Transferrin; D001536:Bence Jones Protein