[Factors for the onset of and the exacerbation of tuberculosis. 1. Activities in cytolysis and in apoptotic induction of mycobacteria on human THP-1 derived macrophage].
Cytolysis resulted in cell death was detected with propidium iodide, and apoptosis with Annexin V by using flow cytometry. The more vilurent strain in known murine infections showed the higher cytolytic activity. However in those mycobacterial strains that had the higher cytolytic activity on THP-1 cell, the lower level of apoptosis induction was observed. Addition of IFN-gamma or TNF-alpha to THP-1 derived macrophage did not effect on cytolytic activities nor apoptosis induction. Since mycobacteria with the higher cytolytic activity showed the lower apoptosis induction, it was suggested that apoptosis of human macrophage in mycobacterial infection is a beneficial phenomenon to the host.
Miyazaki
Y
Y
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Ohno
H
H
Izumikawa
K
K
Tomono
K
K
Kohno
S
S
D017209:Apoptosis; D002478:Cells, Cultured; D018450:Disease Progression; D006801:Humans; D016676:Macrophages, Alveolar / Q000382:microbiology* / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D014376:Tuberculosis / Q000382:microbiology / Q000473:pathology; D014774:Virulence