Lectin-mediated transport of nanoparticles across Caco-2 and OK cells.
Recent experiments by a number of workers have suggested that it may be possible to use various targeting molecules, which bind to the intestinal epithelium, to promote the uptake and transport of nanoparticles from the intestine to the circulation. We have used commercial nanoparticles to examine the effect of size, density and inhibitors on uptake of lectin-coated nanoparticles by epithelial cells. The degree of uptake was most influenced by the density of lectin on the particle, with size and type of lectin being less important. Uptake could be inhibited by the presence of specific sugars or free lectin. These studies should provide a good basis for the design of targetable biodegradable drug-loadable particles suitable for oral delivery.
Russell-Jones
G J
GJ
Biotech Australia Pty Ltd., PO Box 20, Roseville, Australia. greg_russell_jones@mail.bioaust.com.au
Veitch
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H
Arthur
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D000117:Acetylglucosamine / Q000737:chemistry; D001427:Bacterial Toxins / Q000737:chemistry; D001692:Biological Transport; D018938:Caco-2 Cells; D003208:Concanavalin A / Q000737:chemistry; D004768:Enterotoxins / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005677:G(M1) Ganglioside / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D006801:Humans; D037102:Lectins; D007975:Leukotriene B4 / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size; D014909:Wheat Germ Agglutinins / Q000493:pharmacokinetics
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D007975:Leukotriene B4; D005677:G(M1) Ganglioside; C027538:heat-labile enterotoxin, E coli; D016650:Fluorescein-5-isothiocyanate; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose