Quantification of the mycotoxin patulin by a stable isotope dilution assay.
Two stable isotope dilution assays for the quantification of patulin [4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one] in foods were developed using (13)C-labeled patulin as the internal standard. One method was performed by means of LC/MS in negative electrospray ionization mode without derivatization; the other used HRGC/HRMS after trimethylsilylation of the patulin isotopomers. In comparison with previously reported methods based on high-performance liquid chromatography with UV detection, HRGC/HRMS of the derivatized samples showed better repeatability, higher recovery rates (96% at a spike level of 200 ng/L), and a 100 times lower detection limit (12 ng/L). In contrast, LC/MS showed a much lower performance as compared to HPLC/UV or HRGC/HRMS. Using HRGC/HRMS, the mycotoxin was quantified in many different fruit products and in molded wheat bread.
Rychlik
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M
Institut für Lebensmittelchemie der Technischen Universität München, Lichtenbergstrasse 4, D-85748 Garching, Germany.
Schieberle
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P
D001628:Beverages / Q000032:analysis*; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D009183:Mycotoxins / Q000032:analysis; D010365:Patulin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D002247:Carbon Isotopes; D009183:Mycotoxins; D010365:Patulin