Identification of a good c-myc antisense oligodeoxynucleotide target site and the inactivity at this site of novel NCH triplet--targeting ribozymes.
A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate--phosphodiester antisense effectors. Novel inosine--containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA.
Giles
R V
RV
School of Biological Sciences, University of Liverpool.
Spiller
D G
DG
Clark
R E
RE
Tidd
D M
DM
D001426:Bacterial Proteins; D003201:Computers; D016373:DNA, Antisense / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D016337:RNA, Catalytic / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016914:Ribonuclease H / Q000378:metabolism; D013301:Streptolysins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D001426:Bacterial Proteins; D016373:DNA, Antisense; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc; D016337:RNA, Catalytic; D012333:RNA, Messenger; D013301:Streptolysins; C025964:streptolysin O; D016914:Ribonuclease H