A lithium chloride-extracted, broad-spectrum-adhesive 42-kilodalton protein of Staphylococcus epidermidis is ornithine carbamoyltransferase.
To identify novel putative staphylococcal adhesins, lithium chloride extraction (an established method for selective surface molecule solubilization) was employed. N-terminal sequencing and functional assays identified a 42-kDa fibronectin-binding protein from Staphylococcus epidermidis as ornithine carbamoyltransferase (OCTase). However, OCTase was not recognizable extracellularly, and this fact together with the fact that LiCl induced DNA release and a decrease in viability suggests that LiCl extraction may not be the method of choice for selective surface molecule extraction from staphylococci.
Hussain
M
M
Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.
Peters
G
G
Chhatwal
G S
GS
Herrmann
M
M
D018829:Adhesins, Bacterial / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000302:isolation & purification; D001431:Bacteriological Techniques; D015153:Blotting, Western; D002352:Carrier Proteins / Q000302:isolation & purification; D002408:Catheters, Indwelling / Q000382:microbiology; D003030:Coagulase / Q000378:metabolism; D006801:Humans; D018021:Lithium Chloride; D008969:Molecular Sequence Data; D009954:Ornithine Carbamoyltransferase / Q000737:chemistry* / Q000302:isolation & purification*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000201:enzymology* / Q000302:isolation & purification
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; C078635:fibronectin-binding proteins, bacterial; D009954:Ornithine Carbamoyltransferase; D018021:Lithium Chloride