title: Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease.
abstract: We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Iwata
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: R H
authors.initials: RH
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Wakabayashi
authors.forename: M
authors.initials: M
authors.lastname: Takashima
authors.forename: H
authors.initials: H
authors.lastname: Kido
authors.forename: K
authors.initials: K
authors.lastname: Nagashima
authors.forename: K
authors.initials: K
authors.lastname: Kenney
authors.forename: C J
authors.initials: CJ
authors.lastname: Gibbs
authors.forename: T
authors.initials: T
authors.lastname: Kurata
mesh_terms: D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D007536:Isomerism; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D011506:Proteins; D014446:Tyrosine 3-Monooxygenase