Recovery of Salmonella by using selenite brilliant green sulfa enrichment broth.
The efficacy and sensitivity of selenite brilliant green sulfa enrichment (SBG) broth for the isolation of Salmonella from fecal specimens were evaluated by using both clinical and artificially infected (artificial) fecal specimens. An examination of 1,588 clinical fecal specimens found Salmonella in 296 specimens, including 89 cases detected by the direct-plating xylose-lysine-desoxycholate method and an additional 207 cases detected after enrichment with SBG broth. Therefore, the recovery of Salmonella with SBG broth is increased 3.3-fold over that by the direct-plating method alone. Furthermore, the isolation rate of Salmonella is higher when using SBG broth than when using gram-negative (GN) broth or GN broth supplemented with sodium selenite. To determine the sensitivity for the recovery of Salmonella, artificial specimens containing various amounts of Salmonella were prepared and analyzed. The results indicated that the sensitivity is also higher with SBG broth than with GN broth. Moreover, the optimal incubation period for SBG broth can be extended to 24 h. In conclusion, the SBG enrichment method provides a higher recovery rate of Salmonella from fecal specimens.
Chang
C t
Ct
Clinical Laboratory, Kaohsiung 802, Department of Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.
Yuo
C Y
CY
Shen
H C
HC
Li
A M
AM
Chen
C y
Cy
Chou
J l
Jl
Huang
S p
Sp
D001431:Bacteriological Techniques; D004396:Coloring Agents; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D005767:Gastrointestinal Diseases / Q000382:microbiology; D006801:Humans; D000644:Quaternary Ammonium Compounds; D012475:Salmonella / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000382:microbiology*; D012680:Sensitivity and Specificity; D018038:Sodium Selenite; D013449:Sulfonamides
D004396:Coloring Agents; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D013449:Sulfonamides; C006798:brilliant green; D018038:Sodium Selenite