Inverted ratio of m-fas/s-fas expression in early onset pauciarticular juvenile chronic arthritis.
The expression of m-fas/apo1 (CD95) and its soluble form (s-fas) was studied in patients with early onset pauciarticular (n = 23) and systemic juvenile chronic arthritis (n = 7). RNA was prepared from peripheral blood of patients and 22 healthy controls and from 23 samples derived from the synovial fluid of JCA patients. The ratio of m-fas/s-fas transcripts was calculated by densitometry of fas-specific RT-PCR products. An inverted ratio of m-fas/s-fas transcripts was found in PBMC and mononuclear cells from the joints of early onset pauciarticular JCA patients. The m-fas/s-fas ratios were similar in PBMC and mononuclear cells from synovial fluid. PBMC from patients with a systemic JCA showed the same m-fas/s-fas ratio as healthy controls, with an inverted ratio of both transcripts in cells from the synovial fluid. The data indicate a role of Fas/Apo1 regulated apoptosis in EOPA-JCA which is not limited to the affected joints.
Haas
J P
JP
Children's Hospital, Friedrich Alexander University, Erlangen Nuremberg, Germany.
Frank
C
C
Haefner
R
R
Herrmann
M
M
Späth
H
H
Ruder
H
H
D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005786:Gene Expression Regulation; D006801:Humans; D009504:Neutrophils / Q000276:immunology; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics; D013582:Synovial Fluid / Q000276:immunology; D019014:fas Receptor / Q000235:genetics* / Q000378:metabolism
D020033:Protein Isoforms; D019014:fas Receptor