title: Comparison between transient isotachophoretic capillary zone electrophoresis and reversed-phase liquid chromatography for the determination of peptides in plasma.
abstract: Low levels of peptide drugs in human plasma can be determined employing off-line solid-phase extraction, followed by capillary zone electrophoresis with UV detection. A bioanalytical procedure is presented, using gonadorelin and angiotensin II in human plasma as model compounds. The solid-phase extraction method, based on a weak cation exchange mechanism, is able to remove interfering endogenous components from the plasma sample, extract the model peptides quantitatively, and give a possibility of concentrating the sample at the same time. Transient isotachophoretic conditions were kept to increase the sample loadability by about two orders of magnitude. Up to about 70% of the capillary was filled with the reconstituted extract, whereafter the peptides were selectively concentrated during the first 15 min. Subsequently, the concentrated sample zones were separated under capillary zone electrophoresis conditions, showing the technique's high resolution. For the model cationic peptides (gonadorelin, angiotensin II) good linearity and reproducibility was observed in the 20-100 ng/mL concentration range. A more extensive washing procedure permits quantitation of gonadorelin at the 5 ng/mL level. In comparison with a liquid chromatography analysis, superior mass sensitivity and separation are obtained with the transient isotachophoretic capillary zone electrophoresis method. Moreover, in this case equivalent sensitivity is achieved when it is directly compared with a liquid chromatography method with UV detection, keeping in mind that 60 times more sample is needed for the latter method. A further gain in sensitivity can be obtained when the analysis is combined with native fluorescence detection, as is demonstrated by combining liquid chromatography separation with fluorescence detection.
authors.affiliation: Universiteit Utrecht, Faculty of Pharmacy, Department of Pharmaceutical Analysis, The Netherlands. j.c.m.waterval@pharm.uu.nl
authors.forename: J C
authors.initials: JC
authors.lastname: Waterval
authors.forename: H
authors.initials: H
authors.lastname: Krabbe
authors.forename: J
authors.initials: J
authors.lastname: Teeuwsen
authors.forename: A
authors.initials: A
authors.lastname: Bult
authors.forename: H
authors.initials: H
authors.lastname: Lingeman
authors.forename: W J
authors.initials: WJ
authors.lastname: Underberg
mesh_terms: D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D006801:Humans; D010455:Peptides / Q000097:blood*
chemical_list: D010455:Peptides