title: Accumulation of aberrant Y chromosomes in gamma-ray-induced thymic lymphomas lacking p53.
abstract: Male F(1) hybrids between MSM mice carrying a deficient p53 allele and BALB/c mice were irradiated with gamma-rays, and 80 thymic lymphomas were obtained, 46 of which developed in mice carrying the deficient p53 allele. Because the Y chromosome contributes little to cellular function, the stability of the Y chromosome in the tumors was assessed by polymerase chain reaction by examining three genes: Smcy and Sry on the short arm and Sts in the pseudoautosomal region of the long arm of the Y chromosome. Twenty-one lymphomas had lost one or two genes, probably as a result of mitotic recombination or interstitial deletion, whereas no lymphomas had lost all three genes. The p53 status of the lymphomas was determined by genotyping and allelic loss analysis; 34 had retained two wild-type p53 alleles, suggesting normal function; 34 had lost both alleles, indicating loss of function; and the other 12 had at least one wild-type p53 allele, so their p53 status was unclear. Compilation of these data revealed that changes in the Y chromosome were detected in only two of the 34 lymphomas retaining functional p53 but in 18 of the 34 lymphomas lacking p53 function, suggesting that p53 deficiency leads to an increase in the accumulation of radiation-induced aberrant chromosomes. This is consistent with our previous result from analysis of the inactive X chromosome. In contrast, a decrease in the fidelity of mitotic transmission in p53-deficient lymphomas was not noted for the Y chromosome.
authors.affiliation: Department of Biochemistry, Niigata University School of Medicine, Niigata, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Chou
authors.forename: J
authors.initials: J
authors.lastname: Matsuki
authors.forename: Y
authors.initials: Y
authors.lastname: Saitou
authors.forename: S I
authors.initials: SI
authors.lastname: Kosugi
authors.forename: T
authors.initials: T
authors.lastname: Shinbo
authors.forename: F
authors.initials: F
authors.lastname: Gejyo
authors.forename: O
authors.initials: O
authors.lastname: Niwa
authors.forename: R
authors.initials: R
authors.lastname: Kominami
mesh_terms: D000016:Abnormalities, Radiation-Induced / Q000235:genetics*; D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics*; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D013953:Thymus Neoplasms / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53