title: Enzymatic synthesis of 4-amino-3,5-diethylphenyl sulfate, a rodent metabolite of alachlor.
abstract: Rat liver tissue homogenates were utilized for in vitro enzymatic conversion of 2,6-diethylaniline (DEA) to the important alachlor metabolite 4-amino-3,5-diethylphenyl sulfate (ADEPS), which was also generated as a radiolabeled standard for use in metabolism studies. ADEPS formation in rodents is associated with the production of other reactive metabolites implicated in alachlor rodent carcinogenesis, making dependable access to an ADEPS standard highly desirable. (14)C-DEA was oxidized by rat liver microsomes to (14)C-4-amino-3,5-diethylphenol, which was further converted to ADEPS via addition of the phosphosulfate transferase cofactor adenosine-3'-phosphate-5'-phosphosulfate. Microprobe NMR was used in conjunction with high-resolution mass spectrometry to fully characterize the resulting (14)C-ADEPS and confirm its structure. Because microgram quantities sufficed for full characterization, the enzymatic transformation provides a viable alternative to radiosynthesis of (14)C-ADEPS.
authors.affiliation: Monsanto Life Sciences Company, St. Louis, Missouri 63198, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Logusch
authors.forename: P C
authors.initials: PC
authors.lastname: Feng
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: W C
authors.initials: WC
authors.lastname: Hutton
authors.forename: S J
authors.initials: SJ
authors.lastname: Wratten
mesh_terms: D000081:Acetamides / Q000493:pharmacokinetics*; D000473:Alkanes / Q000138:chemical synthesis / Q000378:metabolism*; D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001711:Biotransformation; D002250:Carbon Radioisotopes; D006540:Herbicides / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism*; D051381:Rats; D013431:Sulfates / Q000138:chemical synthesis / Q000378:metabolism*; D013463:Sulfuric Acid Esters
chemical_list: C412087:4-amino-3,5-diethylphenyl sulfate; D000081:Acetamides; D000473:Alkanes; D000814:Aniline Compounds; D002250:Carbon Radioisotopes; D006540:Herbicides; D013431:Sulfates; D013463:Sulfuric Acid Esters; C000188:alachlor; C085691:2,6-diethylaniline