Identification and genetic homogeneity of Trichophyton tonsurans isolated from several regions by random amplified polymorphic DNA.
Trichophyton tonsurans is an anthropophilic dermatophyte mostly causing tinea capitis and tinea corporis. This study was carried out to identify T. tonsurans and to clarify whether it has any genetic differences depending on the phenotype or region of isolation by random amplified polymorphic DNA (RAPD) analysis with three random primers. The assay was performed in 11 Korean, 2 Japanese, 2 Taiwanese, 5 Brazilian and 1 American isolates of T. tonsurans together with the other 10 anamorphic species of dermatophytes and 3 Arthroderma spp. All tested species of dermatophytes showed distinct bands and T. tonsurans was differentiated from other dermatophytes. It was most clearly differentiated from T. mentagrophytes by using primer 5'-GAAGGCTCCC-3' (OPAO-15). No difference was found in RAPD band patterns in all strains of T. tonsurans with these random primers. It was considered that T. tonsurans is a genetically homogeneous species regardless of its isolation regions, morphologic or physiologic characteristics.
Kim
J A
JA
Department of Dermatology, Seoul National University, Korea. jakim@snu.ac.kr
Takizawa
K
K
Fukushima
K
K
Nishimura
K
K
Miyaji
M
M
D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique; D013831:Thiamine / Q000378:metabolism; D014005:Tinea / Q000453:epidemiology / Q000382:microbiology*; D014006:Tinea Capitis / Q000453:epidemiology / Q000382:microbiology; D014249:Trichophyton / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D004271:DNA, Fungal; D013831:Thiamine