Molecular evidence that Reticulomyxa filosa is a freshwater naked foraminifer.
Reticulomyxa filosa is a freshwater protist possessing fine granular, branching and anastomosing pseudopodia and therefore traditionally placed in the class Granuloreticulosea, order Athalamida, as a sister group to the order Foraminiferida. Recent studies have revealed remarkable similarities in pseudopodial motility and ultrastructure between R. filosa and foraminifera (e.g. Allogromia laticollaris), prompting us to conduct a molecular phylogenetic analysis of these seemingly disparate organisms. We sequenced the complete small-subunit of the ribosomal DNA of the cultured strain of R. filosa and compared it to the corresponding sequences of other protists including 12 species of foraminifera. We also sequenced and analyzed the actin coding genes from R. filosa and two species of foraminifera, Allogromia sp. and Ammonia sp. The analysis of both data sets clearly shows that R. filosa branches within the clade of foraminifera, suggesting that R. filosa is in fact a freshwater naked foraminiferan.
Pawlowski
J
J
Department of Zoology and Animal Biology, University of Geneva, Chêne-Bougeries, Switzerland. pawlow@sc2a.unige.ch
Bolivar
I
I
Fahrni
J F
JF
De Vargas
C
C
Bowser
S S
SS
D000199:Actins / Q000235:genetics; D000656:Amoeba / Q000145:classification / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D016054:DNA, Protozoan; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016053:RNA, Protozoan / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D014404:Tubulin / Q000235:genetics
D000199:Actins; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D014404:Tubulin