title: An automated interpretation of MALDI/TOF postsource decay spectra of oligosaccharides. 1. Automated peak assignment.
abstract: A computer program has been developed that helps the interpretation of MALDI/TOF postsource decay (PSD) spectra of N-linked oligosaccharides of a protein. The program includes routines for automated peak assignment and generation of a simulated PSD spectrum. From a raw spectrum, peaks are assigned automatically; i.e., numbers of saccharide residues removed from the parent ion are calculated. If the structure of the oligosaccharide is known, a simulated PSD spectrum of the oligosaccharide will be generated. The simulated PSD spectrum helps interpretation of the observed spectrum. While, in a case where several candidate structures are given, one can narrow the field of plausible structures for the unknown oligosaccharide by comparing the observed spectrum with the simulated PSD spectra. Using a Pentium 233-MHz microprocessor, it takes only a few seconds to interpret a spectrum.
authors.affiliation: Toray Research Center, Inc., Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Sasagawa
authors.forename: N
authors.initials: N
authors.lastname: Dohmae
authors.forename: K
authors.initials: K
authors.lastname: Takio
mesh_terms: D000465:Algorithms; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009844:Oligosaccharides