Analysis of bovine whey proteins in soybean dairy-like products by capillary electrophoresis.
The simultaneous separation of bovine whey proteins [alpha-lactalbumin and beta-lactoglobulin (A+B)] and soybean proteins was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV detection at 280 nm, 15 kV applied voltage, and 30 degrees C temperature. Quantitation of bovine whey proteins in a commercial powdered soybean milk manufactured by adding bovine whey to its formulation was performed using the calibration method of the external standard. Direct injection of a solution of the powdered soybean milk only enabled quantitation of alpha-lactalbumin in the commercial sample. Detection of beta-lactoglobulin (A+B) required acid precipitation of the solution of the sample in order to concentrate bovine whey proteins in the supernatant prior to the analysis of this protein in the whey obtained. Since alpha-lactalbumin could also be quantitated from the injection of the whey, the simultaneous determination of alpha-lactalbumin and beta-lactoglobulin (A+B) was possible upon acid precipitation of the powdered soybean milk solution. Detection limits obtained were 14 microg/g sol. for alpha-lactalbumin and 52 microg/g sol. for beta-lactoglobulin (A+B) which represent protein concentrations about 60 microg/100 g sample for alpha-lactalbumin and 100 microg/100 g sample for beta-lactoglobulin (A+B).
García-Ruiz
C
C
Centro de Tecnología de los Alimentos y Servicios Biosanitarios, Universidad de Alcalá, Alcalá de Henares (Madrid), Spain.
Torre
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M
Marina
M L
ML
D000818:Animals; D002417:Cattle; D003611:Dairy Products / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007768:Lactalbumin / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis*; D030262:Soybean Proteins / Q000032:analysis*
D007782:Lactoglobulins; D030262:Soybean Proteins; D007768:Lactalbumin