title: Dot-ELISA for detection of Salmonella enterotoxin.
abstract: Nineteen strains of Salmonella enterica belonging to seven serovars and two strains of S. bongori were investigated for enterotoxin production by the Chinese hamster ovary (CHO) cell assay and Dot-ELISA. All the 19 strains were found to carry stn gene when tested by polymerase chain reaction (PCR) using specific primers for stn sequence. Thirteen strains (68.42%) were found to produce Salmonella enterotoxin (Stn) when tested by the CHO cell assay. Dot-ELISA could detect Stn in 16 strains (84.21%). Dot-ELISA may be preferred over other assays due to its low cost and simplicity in performance.
authors.affiliation: Robert Koch Institute, Wernigerode, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Rahman
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins; C076733:FlaD protein, Bacteria