Postprandial triacylglycerols from dietary virgin olive oil are selectively cleared in humans.
The aims of the present study were to evaluate the effect of a meal rich in virgin olive oil on triacylglycerol composition of human postprandial triacylglycerol-rich lipoproteins (fraction Sf > 400), and to assess the role of the triacylglycerol molecular species concentration and polarity on lipoprotein clearance. Fasting (0 h) and postprandial blood samples were collected hourly for 7 h from eight healthy normolipidemic subjects after the ingestion of the meal. Plasma and lipoprotein triacylglycerol concentrations increased quickly over fasting values and peaked twice at 2 and 6 h during the 7-h postprandial period. The triacylglycerols in the lipoprotein fraction at 2 h generally reflected the composition of the olive oil, however, the proportions of the individualmolecular species were altered by the processes leading to their formation. Among the major triacylglycerols, the proportion of triolein (OOO; 43.6%) decreased (P < 0.05), palmitoyl-dioleoyl-glycerol (POO; 31. 1%) and stearoyl-dioleoyl-glycerol (SOO; 2.1%) were maintained and linoleoyl-dioleoyl-glycerol (LOO; 11.4%) and palmitoyl-oleoyl-linoleoyl-glycerol (POL; 4.6%) significantly increased (P < 0.05) compared with the composition of the triacylglycerols in the olive oil. Smaller amounts of endogenous triacylglycerol (0.8%), mainly constituted of the saturated myristic (14:0)and palmitic (16:0) fatty acids, were also identified. Analysis of total fatty acids suggested the presence of molecular species composed of long-chain polyunsaturated fatty acids of the (n-3) family, docosapentaenoic acid, [22:5(n-3)] and docosahexaenoic acid (DHA), [22:6(n-3)] and of the (n-6) family [arachidonic acid, [20:4(n-6)]. The fastest conversion of lipoproteins to remnants occurred from 2 to 4 h and was directly related to the concentration of the triacylglycerols in the lipoprotein particle (r = 0.9969, P < 0.05) and not with its polarity (r = 0.1769, P > 0.05). The rates of clearance were significantly different among the major triacylglycerols (OOO, POO, OOL and POL) (P < 0.05) and among the latter ones and PLL (palmitoyl-dilinoleoyl-glycerol, POS (palmitoyl-oleoyl-stearoyl-glycerol) and OLL (oleoyl-dilinoleoyl-glycerol) (P < 0.01). OOO was removed faster and was followed by POO, OOL, POL, PPO (dipalmitoyl-oleoyl-glycerol), SOO, PLL, POS and OLL.
Abia
R
R
Instituto de la Grasa, Consejo Superior de Investigaciones Cientificas, 41012 Sevilla, Spain and Hospital Universitario Virgen del Rocío, 41013 Sevilla, Spain.
Perona
J S
JS
Pacheco
Y M
YM
Montero
E
E
Muriana
F J
FJ
Ruiz-Gutiérrez
V
V
D000328:Adult; D001055:Apolipoproteins B / Q000032:analysis; D004032:Diet; D004435:Eating / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry; D008297:Male; D000069463:Olive Oil; D009994:Osmolar Concentration; D010938:Plant Oils / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*
D001055:Apolipoproteins B; D005227:Fatty Acids; D008074:Lipoproteins; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides