Quantitative evaluation of ehrlichial burden in horses after experimental transmission of human granulocytic Ehrlichia agent by intravenous inoculation with infected leukocytes and by infected ticks.
This paper describes the kinetics of the human granulocytic ehrlichiosis agent in the blood of horses experimentally infected by intravenous inoculation with infected leukocytes and by infected ticks as evaluated by using a real-time quantitative PCR assay. The data obtained indicated differences in the period of incubation, duration of rickettsemia, and initial and maximal ehrlichial loads between the two routes of infection.
Pusterla
N
N
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA. npusterla@ucdavis.edu
Leutenegger
C M
CM
Chae
J S
JS
Lutz
H
H
Kimsey
R B
RB
Dumler
J S
JS
Madigan
J E
JE
D000818:Animals; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000097:blood; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016873:Ehrlichiosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D006734:Horse Diseases / Q000382:microbiology* / Q000473:pathology / Q000635:transmission; D006736:Horses; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D007962:Leukocytes / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods
D004269:DNA, Bacterial