title: Frequent expression of the B-cell-specific activator protein in Reed-Sternberg cells of classical Hodgkin's disease provides further evidence for its B-cell origin.
abstract: The neoplastic cells of classical Hodgkin's disease (cHD), ie, Hodgkin and Reed-Sternberg cells (HRS cells), contain clonally rearranged Ig genes, but are dissimilar to normal B cells in that they mostly do not display B-cell antigens such as CD20 or CD19. The transcription factor B-cell-specific activator protein (BSAP) influences numerous B-cell functions such as B-cell antigen expression, Ig expression, and class switch. We analyzed the expression of BSAP in cHD and control tissues by isotopic in situ hybridization and immunohistochemistry to determine whether BSAP is expressed in HRS cells and, if so, whether it may be involved in the genesis of the abnormal phenotype of these cells. Both in normal lymphoid tissue and non-Hodgkin lymphomas, BSAP transcripts and protein were almost exclusively found in B cells and B-cell lymphomas (40 cases), but were absent from the tumor cells of T-cell neoplasms (41 cases), including 19 cases of anaplastic large cell lymphoma of T- and null-cell type. Among cHD, variable numbers of HRS cells exhibited BSAP transcripts (22 of 25 cases) and protein (28 of 31 cases). Our findings show that BSAP is sufficiently specific to serve as B-cell marker. BSAP expression in HRS cells provides further strong evidence for a frequent B-cell origin of cHD and helps distinguish this disease from anaplastic large cell lymphoma of T- and null-cell type. Because BSAP is much more frequently expressed in HRS cells than the conventional B-cell antigens, the abnormal immunophenotype of HRS cells with frequent absence of B-cell antigens does not appear to be due to absent BSAP expression.
authors.affiliation: Konsultations-und Referenzzentrum für Lymphknoten und Hämatopathologie, the Institute of Pathology, Klinikum Benjamin Franklin, Free University of Berlin, Berlin, Germany.
authors.forename: H D
authors.initials: HD
authors.lastname: Foss
authors.forename: R
authors.initials: R
authors.lastname: Reusch
authors.forename: G
authors.initials: G
authors.lastname: Demel
authors.forename: G
authors.initials: G
authors.lastname: Lenz
authors.forename: I
authors.initials: I
authors.lastname: Anagnostopoulos
authors.forename: M
authors.initials: M
authors.lastname: Hummel
authors.forename: H
authors.initials: H
authors.lastname: Stein
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D006689:Hodgkin Disease / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009687:Nuclear Proteins / Q000096:biosynthesis*; D051757:PAX5 Transcription Factor; D016539:Reed-Sternberg Cells / Q000378:metabolism / Q000473:pathology*; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D014157:Transcription Factors