Beneficial effects of sevoflurane and desflurane against myocardial reperfusion injury after cardioplegic arrest.
OBJECTIVE
To determine whether sevoflurane or desflurane offer additional protective effects against myocardial reperfusion injury after protecting the heart against the ischemic injury by cardioplegic arrest.


METHODS
Isolated rat hearts in a Langendorff-preparation (n = 9) were arrested by infusion of HTK cardioplegic solution and subjected to 30 min global ischemia followed by 60 min reperfusion (controls). An additional 18 hearts were subjected to the same protocol, and sevoflurane (n = 9) or desflurane (n = 9) was added to the perfusion medium during the first 30 min of reperfusion in a concentration corresponding to 1.5 MAC in rats. Left ventricular (LV) developed pressure and creatine kinase (CK) release were determined as indices of myocardial performance and cellular injury, respectively.


RESULTS
The LV developed pressure recovered to 46+/-7% of baseline in controls. Functional recovery during reperfusion was improved by inhalational anesthetics to 67+/-3% (sevoflurane, P<0.05) and 61+/-5% of baseline (desflurane, P<0.05), respectively. Peak CK release during early reperfusion was reduced from 52+/-11 U x min(-1) x g(-1) in controls to 34+/-7 and 26+/-7 U x min(-1) x g(-1) in sevoflurane and desflurane treated hearts, respectively. The CK release during the first 30 min of reperfusion was reduced from 312+/-41 U x g(-1) in control hearts to 195+/-40 and 206+/-37 U x g(-1) in sevoflurane and desflurane treated hearts.


CONCLUSIONS
After ischemic protection by cardioplegia, sevoflurane and desflurane given during the early reperfusion period offer additional protection against myocardial reperfusion injury.
Preckel
B
B
Institut für Klinische Anaesthesiologie and Physiologisches Institut I, Düsseldorf, Germany.
Thämer
V
V
Schlack
W
W
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D000077335:Desflurane; D003971:Diastole / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006324:Heart Arrest, Induced; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008738:Methyl Ethers / Q000494:pharmacology*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane; D016277:Ventricular Function, Left / Q000187:drug effects
D018685:Anesthetics, Inhalation; C039984:Bretschneider cardioplegic solution; D008738:Methyl Ethers; D000077149:Sevoflurane; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D000077335:Desflurane; D007530:Isoflurane; D003402:Creatine Kinase; D005947:Glucose