Enhancement of the UVA induction of haem oxygenase-1 expression by beta-carotene in human skin fibroblasts.
beta-Carotene has often been discussed as a means to reduce the risk of skin photodamage. We studied the antioxidative potential of beta-carotene in human skin fibroblasts exposed to ultraviolet A light. Surprisingly, we found a pro-oxidative effect of beta-carotene. Using the induction of haem oxygenase-1 as a marker for oxidative stress, we found a strong enhancement of gene expression by beta-carotene in ultraviolet A-irradiated cells. This effect was clearly suppressed by concomitant addition of vitamin E but only moderately by vitamin C. The results show that beta-carotene has pro-oxidative properties in human skin fibroblasts exposed to ultraviolet-A light.
Obermüller-Jevic
U C
UC
Department of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, D-70593, Stuttgart, Germany.
Francz
P I
PI
Frank
J
J
Flaccus
A
A
Biesalski
H K
HK
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D006419:Heme Oxygenase (Decyclizing) / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D051547:Heme Oxygenase-1; D006801:Humans; D008565:Membrane Proteins; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000187:drug effects / Q000528:radiation effects; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology; D019207:beta Carotene / Q000494:pharmacology*
D000975:Antioxidants; D018076:DNA, Complementary; D008565:Membrane Proteins; D016877:Oxidants; D012333:RNA, Messenger; D019207:beta Carotene; D014810:Vitamin E; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D001205:Ascorbic Acid