The role of polymorphonuclear leukocytes and oxygen-derived free radicals in experimental acute pancreatitis: mediators of local destruction and activators of inflammation.
Using a retrograde infusion sodium taurocholate pancreatitis model in the rat treatment with oxygen radical scavengers or monoclonal anti-ICAM-1 antibody decreased tissue damage and polymorphonuclear leukocytes (PMN) infiltration. Scavengers or anti-ICAM-1 treatment attenuated the activating capacity of blood PMNs following zymosan stimulation. The local production of oxygen free radicals in the pancreas by systemic infusion of hypoxanthine and regional infusion of xanthine oxidase did not induce acute pancreatitis, although an increase of infiltrating PMNs was observed. Our data suggest that oxygen free radicals and infiltrating PMNs aggravate acute pancreatitis and that both are important mediators of local destruction and systemic activation of PMNs.
Poch
B
B
Department of General Surgery, University of Ulm, Steinhövelstr. 9, 89075, Ulm, Germany.
Gansauge
F
F
Rau
B
B
Wittel
U
U
Gansauge
S
S
Nüssler
A K
AK
Schoenberg
M
M
Beger
H G
HG
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D002374:Catalase / Q000494:pharmacology / Q000627:therapeutic use; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D005609:Free Radicals; D019271:Hypoxanthine / Q000633:toxicity; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D010195:Pancreatitis / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000494:pharmacology / Q000627:therapeutic use; D014969:Xanthine Oxidase / Q000633:toxicity; D015054:Zymosan / Q000633:toxicity
D000911:Antibodies, Monoclonal; D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1; D019271:Hypoxanthine; D015054:Zymosan; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase