Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution.
Analytical and preparative high-speed counter-current chromatography was successfully used for the isolation and purification of hydroxyanthraquinones from Rheum officinale Baill (Dahuang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion and aloe-emodin were isolated each at over 98% purity.
Yang
F
F
Beijing Institute of New Technology Application, China.
Zhang
T
T
Tian
G
G
Cao
H
H
Liu
Q
Q
Ito
Y
Y
D000880:Anthraquinones / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal; D012250:Rheum / Q000737:chemistry*
D000880:Anthraquinones