Gypsy group retrotransposon Tv1 from Drosophila virilis.
We have determined the nucleotide sequence of the 6868 bp full-size retrotransposon termed 'Tv1'. Tv1 was isolated from the DNA fraction of extracellular virus-like particles of Drosophila virilis culture cells. Tv1 has the typical structure for a gypsy-group retrotransposon. The Tv1 element was found to be flanked by 453 bp long terminal direct repeats identical to each other. The central part of the element contains three long open reading frames which resemble the gag, pol and env genes of retroviruses. ORF2 includes conservative motifs of protease, reverse transcriptase, RNase H and integrase in the order characteristic for the gypsy-group retrotransposons. Although most copies of Tv1 are located in pericentromeric heterochromatin, the amplification of this family demonstrated in the cell culture and site polymorphism observed in different Drosophila strains suggest functional activity of the Tv1 element.
Andrianov
B V
BV
N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow. shuppe@vigg.ru
Zakharyev
V M
VM
Reznik
N L
NL
Gorelova
T V
TV
Evgen'ev
M B
MB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004330:Drosophila / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017434:Protein Structure, Tertiary; D018626:Retroelements / Q000235:genetics*; D012469:Salivary Glands / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020079:Terminal Repeat Sequences
D018626:Retroelements; D004247:DNA