Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A.
Variable regulatory subunits of protein phosphatase 2A (PP2A) modulate activity, substrate selectivity and subcellular targeting of the enzyme. We have cloned a novel member of the B type regulatory subunit family, B delta, which is most highly related to B alpha. B delta shares with B alpha epitopes previously used to generate subunit-specific antibodies. Like B alpha, but unlike B beta and B gamma which are highly brain-enriched, B delta mRNA and protein expression in tissues is widespread. B delta is a cytosolic subunit of PP2A with a subcellular localization different from B alpha and may therefore target a pool of PP2A holoenzymes to specific substrates.
Strack
S
S
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Chang
D
D
Zaucha
J A
JA
Colbran
R J
RJ
Wadzinski
B E
BE
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D019556:COS Cells; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D054648:Protein Phosphatase 2; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D015398:Signal Transduction
D010446:Peptide Fragments; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2