Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana.
Synthesis, degradation, and transport of proline (Pro) are thought to cooperatively control its endogenous levels in higher plants in response to environmental conditions. To evaluate the function of Pro degradation in the regulation of the levels of Pro and to elucidate roles of Pro in stress tolerance, we generated antisense transgenic Arabidopsis plants with an AtProDH cDNA encoding proline dehydrogenase (ProDH), which catalyzes Pro degradation. Several transgenic lines accumulated Pro at higher levels than wild-type plants, providing evidence for a key role of ProDH in Pro degradation in Arabidopsis. These antisense transgenics were more tolerant to freezing and high salinity than wild-type plants, showing a positive correlation between Pro accumulation and stress tolerance in plants.
Nanjo
T
T
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, Japan.
Kobayashi
M
M
Yoshiba
Y
Y
Kakubari
Y
Y
Yamaguchi-Shinozaki
K
K
Shinozaki
K
K
D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005615:Freezing; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified; D011392:Proline / Q000502:physiology*; D012965:Sodium Chloride / Q000633:toxicity
D018076:DNA, Complementary; D018744:DNA, Plant; D016376:Oligonucleotides, Antisense; D012965:Sodium Chloride; D011392:Proline