Cellular internalization of PCL(20)-b-PEO(44) block copolymer micelles.
The cellular internalization of polycaprolactone-b-poly(ethylene oxide) (PCL(20)-b-PEO(44)) copolymer micelles were investigated in PC12 cells cultures. The micelles were found to be internalized into PC12 cells when followed over the 4-h incubation period. Also, the internalization process was found to fulfill the basic criteria for endocytotic uptake in that it was time, temperature, pH and energy dependent. In addition, the use of other pharmacological manipulations (hypertonic treatment, Brefeldin A) provide further evidence that the mode of cellular internalization is in fact endocytotic.
Allen
C
C
Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, Que., Canada.
Yu
Y
Y
Eisenberg
A
A
Maysinger
D
D
D000818:Animals; D004337:Drug Carriers / Q000737:chemistry; D004705:Endocytosis; D005027:Ethylene Oxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000737:chemistry*; D008823:Micelles; D008854:Microscopy, Electron; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013696:Temperature
D004337:Drug Carriers; D007783:Lactones; D008823:Micelles; C400421:PLC(20)-b-PEO(44); D011108:Polymers; D005027:Ethylene Oxide