Lipid-dependent activation of protein kinase C-alpha by normal alcohols.
Significant stimulation of protein kinase C-alpha (PKCalpha) by n-alcohols was observed in characterized lipid systems composed of phosphatidylcholine/phosphatidylserine/dioleoylglycerol (PC/PS/DO). The logarithm of the alcohol concentrations to achieve half-maximal PKC stimulation (ED(50)) and of the maximal PKC stimulation by alcohols were both linear functions of alcohol chain length, consistent with the Meyer-Overton effect. Binding of phorbol esters to PKC was not significantly affected by octanol. Octanol increased, up to 4-fold, the affinity of PKC binding to the lipid bilayers in both the absence and presence of DO. However, octanol increased PKC activity much more significantly than it enhanced binding of the enzyme to the lipid bilayers, suggesting that the stimulation of PKC is not merely a reflection of the increase in PKC bilayer binding affinity. (31)P NMR experiments did not reveal formation of non-lamellar phases with octanol. Differential scanning calorimetry suggested that alcohols, like diacylglycerol, induce formation of compositionally distinct domains and the maximal enzyme activity with alcohol resided roughly in the putative domain-coexistence region. These results suggest that alcohols are mimicking diacylglycerol in activating PKC, not by binding to the high affinity phorbol ester binding site, but by altering lipid structure and by enhancing PKC-bilayer binding.
Shen
Y M
YM
Department of Pharmacology, The University of Virginia Health Sciences Center, Charlottesville, Virginia 22903, USA.
Chertihin
O I
OI
Biltonen
R L
RL
Sando
J J
JJ
D000438:Alcohols / Q000737:chemistry / Q000494:pharmacology*; D001667:Binding, Competitive / Q000187:drug effects; D004075:Diglycerides / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D008560:Membrane Fluidity; D000442:Octanols / Q000494:pharmacology; D000439:Pentanols / Q000494:pharmacology; D010703:Phorbol Esters / Q000378:metabolism; D010718:Phosphatidylserines / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D013696:Temperature
D000438:Alcohols; D004075:Diglycerides; D007527:Isoenzymes; D008051:Lipid Bilayers; D008055:Lipids; D000442:Octanols; D000439:Pentanols; D010703:Phorbol Esters; D010718:Phosphatidylserines; C042859:dimyristoylphosphatidylserine; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D004134:Dimyristoylphosphatidylcholine