Localization of the neurokinin 1 receptor on a subset of substance P-positive and isolectin B4-negative dorsal root ganglion neurons of the rat.
Dorsal root ganglion (DRG) neurons that express the neurokinin 1 (NK1) receptor were further characterized immunocytochemically using double-labeling techniques. DRG neurons were isolated from adult rats and cultured for 1-4 days. NK1 receptors on the surface of the neurons were labeled with substance P-gold. Non-peptidergic neurons were labeled with the surface marker isolectin B4 from Griffonia simplicifolia (IB4). Substance P-immunoreactivity was determined using an antibody to substance P. NK1 receptors were not identified on non-peptidergic neurons. The vast majority of the neurons with NK1 receptors showed substance P-immunoreactivity suggesting that NK1 receptors are autoreceptors in many dorsal root ganglion neurons.
von Banchet
G S
GS
Physiologisches Institut, Universität Jena, Germany.
Schaible
H G
HG
D000818:Animals; D015415:Biomarkers; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology*; D037102:Lectins; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000032:analysis*; D013373:Substance P / Q000032:analysis*
D015415:Biomarkers; D037102:Lectins; D018040:Receptors, Neurokinin-1; D013373:Substance P