title: Analysis of NotI linking clones isolated from human chromosome 3 specific libraries.
abstract: We have partially sequenced more than 1000 NotI linking clones isolated from human chromosome 3-specific libraries. Of these clones, 152 were unique chromosome 3-specific clones. The clones were precisely mapped using a combination of fluorescence in situ hybridization (FISH) and hybridization to somatic cell or radiation hybrids. Two- and three-color FISH was used to order the clones that mapped to the same chromosomal region, and in some cases, chromosome jumping was used to resolve ambiguous mapping. When this NotI restriction map was compared with the yeast artificial chromosome (YAC) based chromosome 3 map, significant differences in several chromosome 3 regions were observed. A search of the EMBL nucleotide database with these sequences revealed homologies (90-100%) to more than 100 different genes or expressed sequence tags (ESTs). Many of these homologies were used to map new genes to chromosome 3. These results suggest that sequencing NotI linking clones, and sequencing CpG islands in general, may complement the EST project and aid in the discovery of all human genes by sequencing random cDNAs. This method may also yield information that cannot be obtained by the EST project alone; namely, the identification of the 5' ends of genes, including potential promoter/enhancer regions and other regulatory sequences
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. vlakas@ki.se
authors.forename: V I
authors.initials: VI
authors.lastname: Kashuba
authors.forename: R Z
authors.initials: RZ
authors.lastname: Gizatullin
authors.forename: A I
authors.initials: AI
authors.lastname: Protopopov
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: N V
authors.initials: NV
authors.lastname: Vorobieva
authors.forename: L
authors.initials: L
authors.lastname: Fedorova
authors.forename: V I
authors.initials: VI
authors.lastname: Zabarovska
authors.forename: O V
authors.initials: OV
authors.lastname: Muravenko
authors.forename: M
authors.initials: M
authors.lastname: Kost-Alimova
authors.forename: D A
authors.initials: DA
authors.lastname: Domninsky
authors.forename: C
authors.initials: C
authors.lastname: Kiss
authors.forename: R
authors.initials: R
authors.lastname: Allikmets
authors.forename: V M
authors.initials: VM
authors.lastname: Zakharyev
authors.forename: E A
authors.initials: EA
authors.lastname: Braga
authors.forename: J
authors.initials: J
authors.lastname: Sumegi
authors.forename: M
authors.initials: M
authors.lastname: Lerman
authors.forename: C
authors.initials: C
authors.lastname: Wahlestedt
authors.forename: A V
authors.initials: AV
authors.lastname: Zelenin
authors.forename: D
authors.initials: D
authors.lastname: Sheer
authors.forename: G
authors.initials: G
authors.lastname: Winberg
authors.forename: A
authors.initials: A
authors.lastname: Grafodatsky
authors.forename: L L
authors.initials: LL
authors.lastname: Kisselev
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: E R
authors.initials: ER
authors.lastname: Zabarovsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016208:Databases, Factual; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D020224:Expressed Sequence Tags; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases