title: The activation domain of herpesvirus saimiri R protein interacts with the TATA-binding protein.
abstract: The herpesvirus saimiri open reading frame (ORF) 50 produces two transcripts. The first is spliced, contains a single intron, and is detected at early times during the productive cycle, whereas the second is expressed later and is produced from a promoter within the second exon. Analysis of their gene products has shown that they function as sequence specific transactivators. In this report, we demonstrate that the carboxy terminus of ORF 50b contains an activation domain which is essential for transactivation. This domain contains positionally conserved hydrophobic residues found in a number of activation domains, including the herpes simplex virus VP16 and the Epstein-Barr virus R proteins. Mutational analysis of this domain demonstrates that these conserved hydrophobic residues are essential for ORF 50 transactivation capability. Furthermore, this domain is required for the interaction between the ORF 50 proteins and the basal transcription factor TATA-binding protein.
authors.affiliation: Molecular Medicine Unit, University of Leeds, St. James's University Hospital, Leeds LS9 7TF, United Kingdom.
authors.forename: K T
authors.initials: KT
authors.lastname: Hall
authors.forename: A J
authors.initials: AJ
authors.lastname: Stevenson
authors.forename: D J
authors.initials: DJ
authors.lastname: Goodwin
authors.forename: P C
authors.initials: PC
authors.lastname: Gibson
authors.forename: A F
authors.initials: AF
authors.lastname: Markham
authors.forename: A
authors.initials: A
authors.lastname: Whitehouse
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae; D001665:Binding Sites; D002460:Cell Line; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011817:Rabbits; D020218:Response Elements; D035181:TATA-Box Binding Protein; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation; D014764:Viral Proteins
chemical_list: C068382:BRLF1 protein, Human herpesvirus 4; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins