The Krüppel-like core promoter binding protein gene is primarily expressed in placenta during mouse development.
The human core promoter binding protein (hCPBP) has been identified as a DNA-binding protein involved in the regulation of TATA box-less genes like those encoding the pregnancy-specific glycoproteins. Structurally, hCPBP contains three zinc fingers in the C-terminal domain, which is highly conserved in a number of proteins that constitute the Krüppel-like family of transcription factors. In the present work, we report the molecular cloning of the mouse CPBP (mCPBP) and its expression pattern during development as well as in adult tissues. The mouse cDNA encodes a protein of 283 amino acids that share 94.4% of identity with the hCPBP. The highest level of mCPBP transcript was detected in placenta, and its expression was lower in total embryos and in adult tissues. We also show by in situ hybridization that during embryonic development the mCPBP gene is mainly expressed in extra-embryonic structures throughout gestation; essentially no specific expression was detected in embryonic tissues. Our data demonstrate that CPBP transcript is enriched in the trophoblastic tissue and strongly suggest that its encoded polypeptide regulates target genes involved in placental development and pregnancy maintenance.
Slavin
D
D
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
Sapin
V
V
López-Diaz
F
F
Jacquemin
P
P
Koritschoner
N
N
Dastugue
B
B
Davidson
I
I
Chatton
B
B
Bocco
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000032:analysis; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D016335:Zinc Fingers
C105465:KLF6 protein, human; C491358:Klf6 protein, mouse; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D015534:Trans-Activators