title: Indomethacin-induced G1/S phase arrest of the plant cell cycle.
abstract: In animal systems, indomethacin inhibits cAMP production via a prostaglandin-adenylyl cyclase pathway. To examine the possibility that a similar mechanism occurs in plants, the effect of indomethacin on the cell cycle of a tobacco bright yellow 2 (TBY-2) cell suspension was studied. Application of indomethacin during mitosis did not interfere with the M/G1 progression in synchronized BY-2 cells but it inhibited cAMP production at the beginning of the G1 phase and arrested the cell cycle progression at G1/S. These observations are discussed in relation to the putative involvement of cAMP biosynthesis in the cell cycle progression in TBY-2 cells.
authors.affiliation: Department of Biology, Universiteit Antwerpen (UIA), Universiteitsplein 1, Antwerp, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Ehsan
authors.forename: L
authors.initials: L
authors.lastname: Roef
authors.forename: E
authors.initials: E
authors.lastname: Witters
authors.forename: J P
authors.initials: JP
authors.lastname: Reichheld
authors.forename: D
authors.initials: D
authors.lastname: Van Bockstaele
authors.forename: D
authors.initials: D
authors.lastname: Inzé
authors.forename: H
authors.initials: H
authors.lastname: Van Onckelen
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology; D001549:Benzamides / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D019925:Cyclin A / Q000235:genetics; D004136:Dinitrobenzenes / Q000494:pharmacology; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D006540:Herbicides / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology*; D008940:Mitotic Index / Q000187:drug effects; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000378:metabolism; D016196:S Phase / Q000187:drug effects; D013424:Sulfanilamides; D014026:Nicotiana
chemical_list: D001549:Benzamides; D019925:Cyclin A; D004136:Dinitrobenzenes; D006540:Herbicides; D012333:RNA, Messenger; D013424:Sulfanilamides; C001059:pronamide; D016590:Aphidicolin; C012465:oryzalin; D000242:Cyclic AMP; D007213:Indomethacin