title: Schistosoma mansoni cercarial glycolipids are dominated by Lewis X and pseudo-Lewis Y structures.
abstract: The oligosaccharide structures of glycolipids from cercariae of the human blood fluke, Schistosoma mansoni, were analyzed in the form of their corresponding, pyridylaminated oligosaccharides by methylation analysis, partial hydrolysis, exoglycosidase treatment, on-target exoglyco-sidase cleavage and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The six, dominant chemical structures present have been determined as: GalNAc(beta1-4)Glc1-ceramide; GlcNAc(beta1-3)Gal-NAc(beta1-4)Glc1-ceramide; Gal(beta1-4)GlcNAc(beta1-3)Gal-NAc(beta1-4)Glc1-ceramide; Gal(beta1-4)[Fuc(alpha1-3)]Glc-NAc(beta1-3)Gal-NAc(beta1-4)Glc1-++ +ceram ide (Lewis X pentasaccharide structure); Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-3)Glc-NAc(beta1-3)GalNAc(beta 1-4)Glc1-ceramide (Lewis X hexa-saccharide structure); and, Fuc(alpha1-3)Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-3)GalNAc(beta1-4 )Glc1-ceramide (pseudo-Lewis Y hexasaccharide structure). These structures belong to the characterized schisto-series of protostomial glycosphingolipids. The Lewis X and pseudo-Lewis Y glyco-lipids are stage-specifically expressed by the cercarial life-cycle stage, and not by the adult or egg.
authors.affiliation: Institute of Biochemistry, University of Giessen, D-35392 Giessen, Germany, and.
authors.forename: M
authors.initials: M
authors.lastname: Wuhrer
authors.forename: R D
authors.initials: RD
authors.lastname: Dennis
authors.forename: M J
authors.initials: MJ
authors.lastname: Doenhoff
authors.forename: G
authors.initials: G
authors.lastname: Lochnit
authors.forename: R
authors.initials: R
authors.lastname: Geyer
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism*; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006017:Glycolipids; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen