title: Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-sigma (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas.
abstract: In order to study possible mechanisms leading to chemoresistance in pancreatic adenocarcinoma we examined the global protein expression of pancreatic cancer cells in vitro. We used a cell culture model derived from the adenocarcinoma of the pancreas (EPP85-181P). A classical multidrug-resistant subline, EPP85-181RDB, selected in presence of daunorubicin, and an atypical multidrug-resistant cell variant, EPP85-181RNOV, selected in presence of mitoxantrone, were analyzed using two-dimensional electrophoresis. After staining and image analysis, spots of interest were isolated using preparative two-dimensional electrophoresis and subjected to mass spectrometry and microsequencing. Three proteins, E-FABP, cofilin, and 14-3-3-sigma (stratifin), were overexpressed in chemoresistant cell lines. Cofilin was present in both multidrug in chemoresistant cell lines. Cofilin was present in both multidrug-resistant cell lines. E-FABP and 14-3-3-sigma (stratifin) was found to be overexpressed only in the mitoxantrone-selected atypical multidrug-resistant cell line. The possible significance of these findings is discussed.
authors.affiliation: Institut für Laboratoriums-medizin und Pathobiochemie, Universitätsklinikum Charité, Medizinsche, Fakultät der Humboldt Universität zu Berlin, Germany. pranav.sinha@charite.de
authors.forename: P
authors.initials: P
authors.lastname: Sinha
authors.forename: G
authors.initials: G
authors.lastname: Hütter
authors.forename: E
authors.initials: E
authors.lastname: Köttgen
authors.forename: M
authors.initials: M
authors.lastname: Dietel
authors.forename: D
authors.initials: D
authors.lastname: Schadendorf
authors.forename: H
authors.initials: H
authors.lastname: Lage
mesh_terms: D048948:14-3-3 Proteins; D051339:Actin Depolymerizing Factors; D000230:Adenocarcinoma / Q000378:metabolism*; D014408:Biomarkers, Tumor; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D005092:Exonucleases; D005095:Exoribonucleases; D006801:Humans; D013058:Mass Spectrometry; D008840:Microfilament Proteins / Q000096:biosynthesis*; D009363:Neoplasm Proteins; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D048948:14-3-3 Proteins; D051339:Actin Depolymerizing Factors; D014408:Biomarkers, Tumor; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human