On the Reversible Conjugation of
After administering [17-D(2)]GA(20) to Phaseolus coccineus L. cv. Preisgewinner seedlings, [17-D(2)]GA(20)-O-glucoside was identified by liquid chromatography (LC)/ESI-tandem mass spectrometry (MS). Likewise, by LC/ESI-tandem MS the metabolic formation of [17-D(2)]GA(20) glucosyl ester was established. The application of both [17-D(2)]-labeled GA(20) 13-O-glucoside and GA(20) glucosyl ester to Phaseolus coccineus L. seedlings resulted in free [17-D(2)]GA(20) by gas chromatography/MS. The results demonstrate that conjugation of GA(20) and the reconversion of the glucosyl conjugates are concomitant processes in plants.
Schneider
G
Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/S., Germany
Koch
M
Fuchs
P