Induction of adhesion molecules on human schwann cells by proinflammatory cytokines, an immunofluorescence study.
The presence of cytokines in the peripheral nerve was positively correlated to the induction and progression of inflammation during experimental allergic neuritis (EAN) and Guillain Barré syndrome (GBS). We investigated the induction of adhesion molecules such as L-selectin, E-selectin, ICAM-1, VCAM-1 and Mac-1 on Schwann cells by proinflammatory cytokines. Cultured human Schwann cells from normal adult, fetal and diabetic nerves were studied by immunofluorescence at basal condition and after stimulation with cytokines for 6, 24, 48 and 96 h. Incubation of human Schwann cells with TNFalpha, IFNgamma and IL-1beta induces the expression of ICAM-1 starting at 6 h and reaching a peak at 24 h on more than 90% of cells. VCAM-1 expression was induced after 6 h of treatment with TNFalpha and IL-1beta on almost 100% of Schwann cells. Surprisingly, stimulation with TNFalpha, IFNgamma and IL-1beta also induced the expression of L-selectin on fetal and diabetic Schwann cells, but not on normal adult cells. E-selectin, an adhesion molecule classically upregulated during inflammation, as well as Mac-1, a ligand for ICAM-1, were not expressed on human Schwann cells at basal condition or after treatment with cytokines. No ICAM-1, VCAM-1 and L-selectin expression was found on unstimulated Schwann cells. Our results suggest that upregulation of adhesion molecules on Schwann cells may have a role in the pathogenesis of inflammation in the peripheral nerve.
Constantin
G
G
Department of Neurology, 'Dino Ferrari' Center, University of Milan, IRCCS Ospedale Maggiore Policlinico, Via F. Sforza 35, 20122, Milan, Italy.
Piccio
L
L
Bussini
S
S
Pizzuti
A
A
Scarpini
E
E
Baron
P
P
Conti
G
G
Pizzul
S
S
Scarlato
G
G
D000328:Adult; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D003920:Diabetes Mellitus; D019040:E-Selectin / Q000096:biosynthesis / Q000187:drug effects; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D019041:L-Selectin / Q000096:biosynthesis / Q000187:drug effects; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000187:drug effects; D012583:Schwann Cells / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000187:drug effects
D015815:Cell Adhesion Molecules; D016207:Cytokines; D019040:E-Selectin; D018836:Inflammation Mediators; D007375:Interleukin-1; D016209:Interleukin-8; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D007371:Interferon-gamma