title: A new yeast genus, Tetrapisispora gen. nov.: Tetrapisispora iriomotensis sp. nov., Tetrapisispora nanseiensis sp. nov. and Tetrapisispora arboricola sp. nov., from the Nansei Islands, and reclassification of Kluyveromyces phaffii (van der Walt) van der Walt as Tetrapisispora phaffii comb. nov.
abstract: Seven strains of three new yeast species were isolated from soil, flowers and leaves in the Nansei Islands, Japan. These isolates most closely resembled Kluyveromyces phaffii in physiological characteristics and nuclear DNA base composition (30-32 mol% G + C), but on the basis of DNA-DNA hybridization and electrophoretic karyotyping they were categorized into three new species different from K. phaffii. Phylogenetic analysis using 18S rRNA gene sequences showed that the three new species and K. phaffii were highly related to one another and phylogenetically separate from the members of other species. On the basis of phylogeny and physiological characters, it is proposed that the three new species represent novel taxa and should be designated Tetrapisispora iriomotensis gen. nov., sp. nov. (type strain IFO 10929T), Tetrapisispora nanseiensis gen. nov., sp. nov. (type strain IFO 10899T) and Tetrapisispora arboricola gen. nov., sp. nov. (type strain IFO 10925T), while Kluyveromyces phaffii becomes Tetrapisispora phaffii comb. nov.
authors.affiliation: Institute for Fermentation, Osaka, Japan. kumiueda@mb.infoweb.ne.jp
authors.forename: K
authors.initials: K
authors.lastname: Ueda-Nishimura
authors.forename: K
authors.initials: K
authors.lastname: Mikata
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D007716:Kluyveromyces / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S