title: Cloning and characterization of a cDNA encoding catalase in wheat.
abstract: We determined the nucleotide sequence of a cDNA encoding the catalase (CAT) isolated from wheat (Triticum aestivum L.). The deduced amino acid sequence suggests that this wheat catalase isozyme shared higher amino acid homology with group I CATs of barley CAT-1, rice CAT B and maize CAT-1 and CAT-2 but lower homology with group II CATs of barley CAT-2, rice CAT A and maize CAT-3. Both group I and II specific sequences of Ser-Arg-Leu and Ser-Ser-Ser, respectively considered as peroxisomal targeting signals were found first in monocot plant of wheat. Functionally important amino acids at active center and heme-binding sites detected in all other plant catalase were conserved.
authors.affiliation: Hokkaido Green-Bio Institute, Naganuma, Japan. 68563924@people.or.jp
authors.forename: H
authors.initials: H
authors.lastname: Saruyama
authors.forename: T
authors.initials: T
authors.lastname: Matsumura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D002374:Catalase