title: Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal alpha 2-->3 and alpha 2-->6 sialogalactosylated linkages at subpicomole levels.
abstract: Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAc alpha 2-->3 and alpha 2-->6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405, USA.
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: U
authors.initials: U
authors.lastname: Werner-Zwanziger
authors.forename: D
authors.initials: D
authors.lastname: Wiesler
authors.forename: M
authors.initials: M
authors.lastname: Pagel
authors.forename: M V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D001565:Benzoates / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007093:Imidazoles / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014312:Trisaccharides / Q000737:chemistry
chemical_list: C402101:5-N-acetylneuraminic acid-2-6-lactose alditol; D001565:Benzoates; D007093:Imidazoles; D009844:Oligosaccharides; D011134:Polysaccharides; D012794:Sialic Acids; D014312:Trisaccharides; C102988:benzoic anhydride; C018100:1-methylimidazole