Analysis of sister chromatid exchange and micronuclei in peripheral blood lymphocytes of nurses handling cytostatic drugs.
The genotoxic effect of occupational exposure of 20 nurses who handled cytostatic drugs in medical oncology and haematology units was evaluated by micronucleus and sister chromatid exchange test. The duration of employment in the units and of exposure to cytostatics ranged from 1 to 31 years. The exposed nurses manifested an increase in cells with micronuclei as compared to the control group (P < 0.05). Nurses exposed to cytostatic drugs for 20-31 years showed a higher frequency of micronuclei (P < 0.05), whereas there was no difference in frequencies between the control group and the group exposed for 1-14 years (P > 0.05). The influence of the exposure period proved to be a significant parameter for the micronucleus test. No statistically significant differences were observed in sister chromatid exchange (P > 0.05).
Kasuba
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V
Laboratory for Mutagenesis, Institute for Medical Research and Occupational Health, St. 2 Ksaverska, PO Box 291, 10001 Zagreb, Croatia.
Rozgaj
R
R
Garaj-Vrhovac
V
V
D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure*; D015162:Micronucleus Tests; D008875:Middle Aged; D009729:Nursing; D016273:Occupational Exposure / Q000009:adverse effects*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000970:Antineoplastic Agents