Sphingosine-1-phosphate modulates growth and adhesion of ovarian cancer cells.
Sphingosine-1-phosphate (S1P) is a bioactive lipid molecule. It stimulates the growth of some cells, but inhibits the growth of others. In this study, we describe the detection of sub-microM to microM concentrations of S1P in the ascitic fluids of patients with ovarian cancer. In ovarian cancer cells cultured in vitro, S1P exhibited a dual effect on growth and/or survival. S1P (10 microM) induced cell death when cells were in suspension but stimulated cell growth when cells were attached. The calcium-dependent induction of cell death by S1P is apparently associated with its inhibitory effect on cell attachment and cell adhesion. S1P (10-30 microM) also induced calcium-dependent cell-cell aggregation.
Hong
G
G
Department of Cancer Biology, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195, USA.
Baudhuin
L M
LM
Xu
Y
Y
D001201:Ascites / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D008246:Lysophospholipids; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000502:physiology; D014407:Tumor Cells, Cultured
D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D013110:Sphingosine