title: Identification of a common site of provirus integration in radiation leukemia virus-induced T-cell lymphomas in mice.
abstract: The BL/VL(3) Kaplan radiation leukemia virus (RadLV-VL(3)) is a nondefective retrovirus that induces T cell lymphomas in several strains of mice. By using DNA probes derived from RadLV/VL(3) provirus-flanking sequences cloned from the BL/VL(3) cell line, we identified a DNA region rearranged in 5 of 19 tumors analysed (25%). All proviruses were integrated in the same 5'-to-3' orientation in a small DNA region called Kis1 (Kaplan integration site 1). This region was localized on distal mouse chromosome 2 in a region not previously identified as important to lymphomagenesis. The cells rearranged at the Kis1 locus represent a clonal subpopulation of the clonal tumor masses examined, indicating a probable role of Kis1 in tumor progression.
authors.affiliation: Département des Sciences Biologiques, Université du Québec à Montréal, Québec, H3C-3P8, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Lambert
authors.forename: D
authors.initials: D
authors.lastname: Bergeron
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: E
authors.initials: E
authors.lastname: Rassart
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009115:Muridae; D011533:Proviruses / Q000235:genetics*; D016721:Radiation Leukemia Virus / Q000235:genetics*; D015183:Restriction Mapping; D012192:Retroviridae Infections / Q000235:genetics / Q000821:virology; D013601:T-Lymphocytes / Q000821:virology; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000235:genetics / Q000821:virology; D016662:Virus Integration