Glutamine synthetase activity in Solanaceous cell suspensions accumulating alkaloids or not. 13C NMR and enzymatic assay.
The metabolism of labelled pyruvate followed by 13C NMR and the measure of glutamine synthetase (GS) showed, according to previous results, a high activity of this enzyme in suspension cells of Nicotiana plumbaginifolia. This activity could derive glutamate from the alkaloid synthesizing pathways. However, a recent work showed that the rate of the GS gene transcription was inversely proportional to the Gln/Glu ratio. The measures of Gln and Glu concentrations in Nicotiana plumbaginifolia cells revealed that high GS activity correlates with the weak value of Gln/Glu ratio. Therefore, the hypothesis of GS dysfunctioning for the non-biosynthesis of alkaloids in N. plumbaginifolia suspension cells can be discarded. This conclusion is strengthened by the results obtained when using a GS inhibitor.
Mesnard
F
F
Laboratoire de biophysique, faculté de pharmacie, Amiens, France.
Marty
D
D
Gillet-Manceau
F
F
Fliniaux
M A
MA
Monti
J P
JP
D000470:Alkaloids / Q000096:biosynthesis*; D002478:Cells, Cultured; D013286:Datura stramonium / Q000235:genetics / Q000378:metabolism*; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018698:Glutamic Acid / Q000096:biosynthesis*; D005973:Glutamine / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D008717:Methionine Sulfoximine / Q000494:pharmacology; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D013045:Species Specificity; D013535:Suspensions; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
D000470:Alkaloids; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D013535:Suspensions; D005973:Glutamine; D008717:Methionine Sulfoximine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005974:Glutamate-Ammonia Ligase