Characterization of the molecular species of phosphatidylethanolamine from kidney of the fresh water snail Lymneae stagnalis by mass spectrometry.
The structural analysis of sixteen molecular species of diacyl glycerophosphoethanolamine from fresh water snail Lymneae stagnalis kidney using chromatography and mass spectrometry is described in this paper. 1-eicosadienoyl-2-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine (PE 20:2-20:4), 1-eicosapentaenoyl-2-eicosadienoyl-sn-glycero-3-phosphoethanolamine (PE 20:5-20:2) and 1-eicosatrienoyl-2-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine (PE 20:3-20:4) as well as 1-octadecanoyl-2-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine (PE 18:0-20:4), 1-ocetadecenoyl-2-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine (PE 18:1-20:4) and 1-octadecanoyl-2-eicosapentaneoyl-sn-glycero-3-phosphoethanolamine (PE 18:0-20:5) were found as the major molecular species, and the first three were tentatively identified as the novel species present in this biological material. The presence of a relatively high content of 1,2-dieicosenoyl-sn-glycero-3-phosphoethanolamine species (approximately 27% of total species) as well as the absence of 22-carbon fatty acid containing and plasmalogen PE molecular species are remarkable in healthy Lymneae stagnalis kidney. Copyright 1999 John Wiley & Sons, Ltd.
Chen
S
Department of Pharmacology, Rush Medical College, Chicago, IL 60612, USA.
Carvey
PM
Li
K