Dendritic Ca2+ transient increase evoked by wind stimulus in the cricket giant interneuron.
In vivo Ca2+ imaging was applied to the cricket median giant interneuron (MGI), to visualize the dendritic processing of mechanosensory signals. Wind stimulation (air-puff) to the cerci induced a transient increase in the cytosolic Ca2+ concentration ([Ca2+]i) in the MGI with a latency of a few seconds, suggesting the release of Ca2+ from the intracellular store site in the MGI. The amplitude of the transient increase in [Ca2+]i in the dendrites depended on the direction of the air-puff, and the increase in [Ca2+]i evoked by the air-puff was suppressed by the hyperpolarizing current injection which blocked the generation of action potentials. These results indicate that the action potential is necessary to the direction-sensitive increase in [Ca2+]i induced by wind stimulation.
Ogawa
H
H
Kawachi Millibioflight Project, ERATO, Meguro, Tokyo, Japan. hogawa@saitama-med.ac.jp
Baba
Y
Y
Oka
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D017952:Ganglia, Invertebrate / Q000166:cytology; D006135:Gryllidae; D066298:In Vitro Techniques; D007395:Interneurons / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008564:Membrane Potentials; D008839:Microelectrodes; D010812:Physical Stimulation; D014919:Wind
D002118:Calcium