title: Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus.
abstract: The complete nucleotide sequence (6043 nt) of RNA 1 from Potato mop-top virus (PMTV-Sw), the type member of the genus Pomovirus, was determined. The first (5'-terminal) open reading frame (ORF 1) encodes a predicted protein of 148 kDa. ORF 2 extends through the opal stop codon of ORF 1 producing a predicted readthrough protein of 206 kDa which resembles the RNA-dependent RNA polymerases (RdRp) of other fungal-transmitted viruses. It includes a methyltransferase, a helicase and a GDD RdRp motif, respectively. Phylogenetic analyses of RdRps indicated that PMTV is most closely related to Beet soil-borne virus (genus Pomovirus), Broad bean necrosis virus (genus Pomovirus) and Soil-borne wheat mosaic virus (genus Furovirus), and is more distantly related to the other viruses of the former furovirus group. The 5' and 3' termini of RNA 1 in PMTV contained untranslated regions (UTR) of 114 nt and 489 nt, respectively. The 3'-UTR of RNA 1 contained a tRNA-like structure, which has previously been reported in the 3'-UTR of RNA 2 but not RNA 3. However, in this study, the tRNA-like structure was also found in the 3'-UTR of RNA 3, which confirms its presence in the 3'-UTRs of all three RNAs of PMTV.
authors.affiliation: Department of Plant Biology, Genetic Centre, SLU, PO Box 7080, S- 750 07 Uppsala, Sweden1.
authors.forename: Eugene I
authors.initials: EI
authors.lastname: Savenkov
authors.affiliation: Department of Plant Biology, Genetic Centre, SLU, PO Box 7080, S- 750 07 Uppsala, Sweden1.
authors.forename: Maria
authors.initials: M
authors.lastname: Sandgren
authors.affiliation: Department of Plant Biology, Genetic Centre, SLU, PO Box 7080, S- 750 07 Uppsala, Sweden1.
authors.forename: Jari P T
authors.initials: JPT
authors.lastname: Valkonen
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001483:Base Sequence; D004279:DNA, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics*; D012343:RNA, Transfer / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000821:virology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D004279:DNA, Viral; D012367:RNA, Viral; D012343:RNA, Transfer