Inhibition of human small cell lung cancer cell growth by methylprednisolone.
Methylprednisolone (MP) inhibited cancer cell growth at concentrations between 0 and 2.0 mM. IC50 was 0.96 mM in H82, 0.44 mM in H345, 0.86 mM in H510A, 0.54 mM in N592 and 0.52 mM in H2081. The growth inhibition was not disturbed by addition of 1 microM RU38486. DNA fragmentation were observed in H510A and N592 at MP concentration of 0.2 mM. Glucocorticoid (GC) receptor mRNA expression was detectable only in H510A. We concluded that MP has potency as an anti-cancer agent at a high concentration. The effect was probably not through GC receptor binding, and growth inhibition was independent of apoptosis induction.
Yamaguchi
S
S
First Department of Medicine, Asahikawa Medical College, Asahikawa 078-8510, Japan.
Ohsaki
Y
Y
Nishigaki
Y
Y
Toyoshima
E
E
Kikuchi
K
K
D001667:Binding, Competitive / Q000187:drug effects; D018288:Carcinoma, Small Cell / Q000188:drug therapy* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008775:Methylprednisolone / Q000494:pharmacology*; D015735:Mifepristone / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D014316:Tritium; D015735:Mifepristone; D003907:Dexamethasone; D008775:Methylprednisolone