[A study on expression of autotaxin mRNA in hepatocellular carcinoma].
OBJECTIVE
To know if ATX is expressed in hepatocellular carcinoma.


METHODS
7721 hepatoma cell lines in culture, 5 normal liver tissues, and 32 histologically verified HCC specimens were obtained. Semiquantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX.


RESULTS
The cDNA sequence of RT-PCR product of 7721 hepatoma cell lines was measured, it showed that nucleotides measured were 332 bp. ATX cDNA nucleotide sequence in HCC was found to share 99.7% homology with that in Melanoma. ATX was expressed in all of the 32 HCC and 5 normal liver tissues. The mean expression level of ATX gene in HCC samples was higher than that in normal liver tissues (0.68 +/- 0.1 vs 0.3 +/- 0.08, P < 0.01).


CONCLUSIONS
ATX gene was found to be in existence in HCC and normal liver tissues. The homology of ATX cDNA sequence between HCC and human Melanoma was 99.7%, and ATX may play an important role in HCC metastatic mechanism.
Zhao
Z
Z
Department of Gastroenterology, First Affiliated Hospital, Nanjing Medical University.
Xu
S
S
Zhang
G
G
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured; D000099189:Lysophospholipase D
D018076:DNA, Complementary; D005956:Glucose-6-Phosphate Isomerase; D006023:Glycoproteins; D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D012333:RNA, Messenger; D000099189:Lysophospholipase D