Use of the Actinobacillus actinomycetemcomitans leukotoxin promoter to drive expression of the green fluorescent protein in an oral pathogen.
The gene for the green fluorescent protein, gfp, was cloned, under the control of the Actinobacillus actinomycetemcomitans leukotoxin (ltx) promoter, in the A. actinomycetemcomitans shuttle vector, pSU20. A actinomycetemcomitans containing the ltx-gfp construct emitted bright green fluorescence in the standard invasion assay using epifluorescence microscopy. These data demonstrate that the green fluorescent protein will be a useful tool for the live analysis of A. actinomycetemcomitans interactions with host cells, and that the ltx promoter can be used to drive the expression of non-A. actinomycetemcomitans genes.
Lippmann
J E
JE
Department of Microbiology and Molecular Genetics, College of Medicine, University of Vermont, Burlington 05405, USA.
Froeliger
E H
EH
Fives-Taylor
P M
PM
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics* / Q000378:metabolism*; D001431:Bacteriological Techniques; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005098:Exotoxins / Q000235:genetics; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic
D005098:Exotoxins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; C018579:leukotoxin; D049452:Green Fluorescent Proteins