Dependence of echovirus 9 on the enterovirus RNA replication inhibitor 2-(alpha-Hydroxybenzyl)-benzimidazole maps to nonstructural protein 2C.
HBB [2-(alpha-hydroxybenzyl)-benzimidazole] selectively inhibits RNA synthesis of most enteroviruses. However, isolation of HBB-dependent variants is possible. Sequence analysis and characterization of recombinant viruses revealed that HBB dependence maps to the nonstructural protein 2C. A single point mutation at position C(4782)U is sufficient to establish the HBB-dependent phenotype in our echovirus 9 model.
Hadaschik
D
D
Institut für Virologie der Universität zu Köln, 50935 Cologne, Germany.
Klein
M
M
Zimmermann
H
H
Eggers
H J
HJ
Nelsen-Salz
B
B
D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D003001:Cloning, Molecular; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D006801:Humans; D017354:Point Mutation; D020365:RNA Helicases / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins
D000998:Antiviral Agents; D001562:Benzimidazoles; D012367:RNA, Viral; D014764:Viral Proteins; C021033:HBBPC; C116598:2C helicase; D020365:RNA Helicases