Regeneration of transected spinal cord in young adult rats using freeze-dried alginate gel.
We have recently reported that freeze-dried alginate gel, which was developed in our laboratory, enhanced peripheral nerve regeneration. The purpose of this study was to examine whether alginate gel is capable of promoting nerve regeneration in the severed spinal cord of adult mammals. Using Wistar rats at 30 days of age (P30), the T9-T10 spinal cord was totally resected and alginate gel was implanted across the gap. Forty-five days after surgery myelinated and unmyelinated axons regenerated throughout the gap with remaining alginate gel. The elongated axons established electrophysiologically functional projections across the gap. In conclusion, freeze-dried alginate gel could be a promising material as an artificial nerve guide for repair of injured central nervous system.
Suzuki
K
K
Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Japan.
Suzuki
Y
Y
Ohnishi
K
K
Endo
K
K
Tanihara
M
M
Nishimura
Y
Y
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000502:physiology; D002818:Cordotomy; D004594:Electrophysiology; D019054:Evoked Potentials, Motor / Q000502:physiology; D005612:Freeze Drying; D005782:Gels; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*
D000464:Alginates; D005782:Gels