Protective effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase, against laryngeal injury in rats.
The effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase activity, was evaluated in a rat model of laryngeal injury induced by endotracheal intubation for 1 h. At 1 h after extubation, the laryngeal damage was characterized by areas of mucosal necrosis, submucosal edema, swelling of subglottic glands, and submucosal infiltration of inflammatory cells. Activity of myeloperoxidase, a marker of neutrophil infiltration, was also markedly increased into the damaged tissue. Immunohistochemistry for nitrotyrosine, an index of nitrosative stress, showed an intense staining in the inflamed larynx. Treatment with 3-aminobenzamide (10 mg/kg intraperitoneally) significantly reduced the appearance of mucosal damage and was associated with a significant reduction of tissue myeloperoxidase activity and nitrotyrosine immunoreactivity in the larynx. The results of this study suggest that poly (ADP-ribose) synthetase may play a role in the inflammatory process after laryngeal intubation and extubation, and administration of 3-aminobenzamide may be a beneficial therapeutic approach.
Stern
Y
Y
Department of Otolaryngology, Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Salzman
A
A
Cotton
R T
RT
Zingarelli
B
B
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007150:Immunohistochemistry; D007442:Intubation, Intratracheal / Q000009:adverse effects*; D007820:Laryngeal Mucosa / Q000737:chemistry / Q000473:pathology; D007830:Larynx / Q000293:injuries* / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D009195:Peroxidase / Q000032:analysis; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051381:Rats; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis; D014947:Wounds and Injuries / Q000209:etiology / Q000473:pathology
D001549:Benzamides; D004791:Enzyme Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C002744:3-nitrotyrosine; D014443:Tyrosine; C025160:3-aminobenzamide; D009195:Peroxidase