Role of protein kinases A and C in the induction of mGluR-dependent long-term depression in the medial perforant path of the rat dentate gyrus in vitro.
The involvement of protein kinases A and C in the induction of low frequency stimulation-induced long-term depression (LTD) in the medial perforant path of the dentate gyrus in vitro has been studied using the selective PKA inhibitors H-89 and KT 5720 and PKC inhibitors Bisindolylmaleimide and Ro-31-8220. The PKC inhibitors Bisindolylmaleimide I and Ro-31-8220 and the PKA inhibitors H-89 and KT5720 all partially inhibited LTD induction. However, the presence of both a PKC and a PKA inhibitor was necessary to completely block LTD induction. The induction of long-term potentiation was not blocked by the inhibitors. It is suggested that the induction of LTD by LFS involves activation of PKC and PKA following activation of group I and group II metabotropic glutamate receptors (mGluR).
Huang
L Q
LQ
Department of Physiology, Trinity College, Dublin, Ireland.
Rowan
M J
MJ
Anwyl
R
R
D000818:Animals; D002227:Carbazoles; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018891:Dentate Gyrus / Q000737:chemistry / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D017774:Long-Term Potentiation / Q000502:physiology*; D008301:Maleimides / Q000494:pharmacology; D009433:Neural Inhibition / Q000502:physiology; D019580:Perforant Pathway / Q000737:chemistry / Q000201:enzymology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000502:physiology*; D013449:Sulfonamides
D002227:Carbazoles; D004791:Enzyme Inhibitors; D007211:Indoles; D007546:Isoquinolines; D008301:Maleimides; D011758:Pyrroles; D018094:Receptors, Metabotropic Glutamate; D013449:Sulfonamides; C057416:KT 5720; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C088060:bisindolylmaleimide; C064758:Ro 31-8220