Prognostic value of DNA image cytometry in resected colorectal hepatic metastases.
OBJECTIVE
To determine the role of DNA image cytometry (DNA ICM) as a useful predictor of outcome following the resection of colorectal hepatic metastases.


METHODS
In 75 patients (56 R0 resections) with resected colorectal hepatic metastases, DNA ICM was performed on paraffin-embedded specimens. The DNA content of 250 tumor cells was determined in each specimen, and the 2c level was evaluated using 30 granulocytes from the same sample.


RESULTS
Common algorithms of DNA ICM, such as maximum DNA content, 5c exceeding rate, 9c exceeding rate, 2c deviation index and the DNA grade of malignancy, identified a group of patients with favorable survival following R0 resection. Clinical findings failed to serve as a prognostic factor. A multivariate analysis revealed the maximum DNA content to be an independent factor influencing postoperative survival.


CONCLUSIONS
DNA ICM is associated with the biologic aggressiveness of colorectal hepatic metastases and is useful as a prognostic marker in patients after resection.
Riesener
K P
KP
Department of Surgery, University Hospital, Rheinisch-Westfälische Technische Hochschule Aachen, Germany.
Füzesi
L
L
Biesterfeld
S
S
Götzen
A
A
Kasperk
R
R
Schumpelick
V
V
D000230:Adenocarcinoma / Q000235:genetics / Q000401:mortality / Q000556:secondary* / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D002467:Cell Nucleus / Q000235:genetics / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000401:mortality / Q000556:secondary* / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006498:Hepatectomy; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007620:Karyometry; D008113:Liver Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011003:Ploidies; D011379:Prognosis; D015996:Survival Rate; D016896:Treatment Outcome
D004273:DNA, Neoplasm