title: Comparison of Aspergillus niger phytase and Trichoderma reesei phytase and acid phosphatase on phytate phosphorus availability in pigs fed on maize-soybean meal or barley-soybean meal diets.
abstract: The efficacy of Aspergillus niger (APhy) phytase, Trichoderma reesei (TPhy) phytase and acid phosphatase (TAcPh) preparations in improving the utilization of phytin-phosphorus in the maize-soybean meal (SBM) or barley-SBM (800:200 g kg-1) diets was studied in two separate digestibility and balance trials with ten growing pigs using 5 x 5 Latin square designs. The positive control diet contained a total phosphorus (P) of 6.5 g kg-1, while the negative control as well as the APhy, TPhy and TAcPh supplemented diets which did not contain additional inorganic-P, had a total P of 4.1 g kg-1. The APhy and TPhy supplements provided phytase activity of 1000 PU g-1 together with AcPh of 8000 HFU g-1. TAcPh at a level of 8000 HFU g-1 was the only addition to one diet. The intrinsic phytase activity of barley was 355 PU g-1 while maize and soybean meal showed no phytase activity. Phytase supplements of the APhy and TPhy sources increased ash digestibility in both diets but had only a minor effect on nitrogen utilization. The addition of phytase improved absorption of P by 21%-units in barley-SBM diet and 29%-units in maize-SBM diet, without any difference between the two phytase sources. The retained P in diets with phytase was higher than in diets without phytase, 4.4 (APhy), 4.5 (TPhy) vs. 2.9 g d-1 in barley-SBM-diets and 3.7 (APhy), 4.0 (TPhy) vs. 1.8 g d-1 in maize-SBM-diets. No difference was found between the two sources of phytase. TAcPh without additional phytase did not show any effect on P absorption or retention. Ca absorption and retention were improved due to the phytase treatments. Supplementing pig diets with either APhy or TPhy sources seems to be equally effective in enhancing the availability of phytate-P. Consequently, these supplements can reduce the P-excretion of pigs by 32-40% as compared with the diet supplemented with inorganic-P.
authors.affiliation: Department of Animal Science, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Näsi
authors.forename: K
authors.initials: K
authors.lastname: Partanen
authors.forename: J
authors.initials: J
authors.lastname: Piironen
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D000135:Acid Phosphatase / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology*; D004063:Digestion / Q000502:physiology; D005503:Food Additives; D001467:Hordeum; D010833:Phytic Acid / Q000378:metabolism*; D013025:Glycine max; D013552:Swine / Q000502:physiology*; D014242:Trichoderma / Q000201:enzymology*; D003313:Zea mays
chemical_list: D005503:Food Additives; D010833:Phytic Acid; D000135:Acid Phosphatase; D010832:6-Phytase