Dot-ELISA for detection of Salmonella enterotoxin.
Nineteen strains of Salmonella enterica belonging to seven serovars and two strains of S. bongori were investigated for enterotoxin production by the Chinese hamster ovary (CHO) cell assay and Dot-ELISA. All the 19 strains were found to carry stn gene when tested by polymerase chain reaction (PCR) using specific primers for stn sequence. Thirteen strains (68.42%) were found to produce Salmonella enterotoxin (Stn) when tested by the CHO cell assay. Dot-ELISA could detect Stn in 16 strains (84.21%). Dot-ELISA may be preferred over other assays due to its low cost and simplicity in performance.
Rahman
H
H
Robert Koch Institute, Wernigerode, Germany.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D012703:Serotyping
D001426:Bacterial Proteins; C076733:FlaD protein, Bacteria