Role of nitric oxide in 2-deoxy-D-glucose-induced hypothermia in rats.
The present study was designed to test the hypothesis that nitric oxide (NO) plays a role in 2-deoxy-D-glucose (2-DG)-induced hypothermia. The body temperature of awake, unrestrained rats was measured before and after the administration of 2-DG, or N(G)-nitro-L-arginine methyl ester (L-NAME; a non-selective NOS inhibitor) or both treatments together. We observed a significant reduction in body temperature after 2-DG injection. L-NAME alone caused no significant change in body temperature. When the two treatments were combined, a reduction in the magnitude of 2-DG-induced hypothermia was observed. The neuronal NOS inhibitor 7-nitroindazole also inhibited 2-DG-induced hypothermia. The data indicate that NO, probably produced by neuronal NOS, plays a role in 2-DG-induced hypothermia.
Carnio
E C
EC
Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, SP, Brasil.
Almeida
M C
MC
Fabris
G
G
Branco
L G
LG
D000818:Animals; D000963:Antimetabolites; D001831:Body Temperature / Q000187:drug effects; D003847:Deoxyglucose; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007035:Hypothermia / Q000139:chemically induced* / Q000503:physiopathology; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D017208:Rats, Wistar
D000963:Antimetabolites; D004791:Enzyme Inhibitors; D007191:Indazoles; D009569:Nitric Oxide; D003847:Deoxyglucose; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; C080122:7-nitroindazole; D019331:NG-Nitroarginine Methyl Ester