title: Listeria monocytogenes possesses adhesins for fibronectin.
abstract: Listeria monocytogenes is a gram-positive, nonsporulating, food-borne pathogen of humans and animals that is able to invade many eukaryotic cells. Several listerial surface components have been reported to interact with eukaryotic cell receptors, but the complete mechanism by which the bacteria interact with all of these cell types remains largely unknown. In this work, we found that L. monocytogenes binds to human fibronectin, a 450,000-Da dimeric glycoprotein found in body fluids, on the surface of cells and in an insoluble component of the extracellular matrix. The binding of fibronectin to L. monocytogenes was found to be saturable and dependent on proteinaceous receptors. Five fibronectin-binding proteins of 55.3, 48.6, 46.7, 42.4, and 26.8 kDa were identified. The 55.3-kDa protein was proved to be present at the bacterial cell surface. The binding of L. monocytogenes to fibronectin adds to the number of molecules to which the bacterium is able to adhere and emphasizes the complexity of host-pathogen interactions.
authors.affiliation: Department of Virology, Pasteur Institute, Brussels, Belgium. pgilot@ben.vub.ac.be
authors.forename: P
authors.initials: P
authors.lastname: Gilot
authors.forename: P
authors.initials: P
authors.lastname: André
authors.forename: J
authors.initials: J
authors.lastname: Content
mesh_terms: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D008089:Listeria monocytogenes / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; C078635:fibronectin-binding proteins, bacterial