title: Surfactant lipid uptake and metabolism by neonatal and adult type II pneumocytes.
abstract: Animal experiments suggest developmental changes in surfactant homeostasis. The uptake and metabolism of [(3)H]dipalmitoylphosphatidylcholine-labeled liposomes with a surfactant-like composition were evaluated in type II cells isolated from rats of different postnatal ages. The early part of the uptake process (0-60 min) was more rapid and reached higher levels in cells from 2-day-old rats than in those from 7-day-old, 14-day-old, or adult rats. Temperature independence of this initial phase, differences in response to trypsin-EDTA or neuraminidase treatment, and the dependency of increased neonatal uptake on the presence of phosphatidylglycerol in liposomes suggested binding as a major mechanism of cell-lipid interaction. Although a two to three times larger amount of lipid was associated with neonatal cells, the metabolism of phosphatidylcholine, indicated by a decrease in label in phosphatidylcholine and an accompanying increase in sphingomyelin, was significantly smaller in 2-day-old than in adult cells. These studies support the hypothesis that neonatal and adult cells may have differences in the interaction with alveolar phospholipids and in the metabolism of phosphatidylcholine.
authors.affiliation: Kinderpoliklinik, Ludwig-Maximilians-University, D-80336 Munich, Germany. griese@pk-i.med.uni-muenchen.de
authors.forename: M
authors.initials: M
authors.lastname: Griese
authors.forename: J
authors.initials: J
authors.lastname: Beck
authors.forename: F
authors.initials: F
authors.lastname: Feuerhake
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism / Q000493:pharmacokinetics; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001993:Bronchodilator Agents / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D008081:Liposomes / Q000378:metabolism; D008854:Microscopy, Electron; D010743:Phospholipids / Q000493:pharmacokinetics; D011650:Pulmonary Alveoli / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013726:Terbutaline / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014316:Tritium
chemical_list: D001993:Bronchodilator Agents; D002273:Carcinogens; D008081:Liposomes; D010743:Phospholipids; D011663:Pulmonary Surfactants; D014316:Tritium; D015060:1,2-Dipalmitoylphosphatidylcholine; D000255:Adenosine Triphosphate; D013726:Terbutaline; D013755:Tetradecanoylphorbol Acetate