Characterization by gas chromatography/mass spectrometry of sterols in saccharomyces cerevisiae during autolysis.
Yeast autolysis affects membrane stability and induces a release of vacuolar enzymes into the cell cytoplasm. Consecutively, it was important to study the evolution of sterol content in Saccharomycescerevisiae for a fourteen day period of accelerated autolysis. Unesterified and esterified sterols were analyzed both in the biomass and in the autolysis medium. Ten sterols were identified by gas chromatography/mass spectrometry. A second group of six sterols was separated and partially characterized. Among the first group of 10 sterols, a dehydroergosterol was identified as ergosta-5, 7,9(11),22-tetraen-3beta-ol, not yet charaterized in S. cerevisiae. Yeast autolysis induced a decrease of esterified sterol content, especially first intermediates in the sequence of the ergosterol biosynthesis, as zymosterol. In contrast, the yeast autolysis resulted in the release of a low quantity of sterols into the medium. At the end of the fourteenth day of autolysis, 0.015% of the total sterol content of the initial biomass was found in the medium.
Le Fur
Y
Y
Laboratoire de Biochimie Alimentaire, ENESAD, B.P. 1607, F-21036 Dijon Cedex, France. y.lefur@enesad.fr
Maume
G
G
Feuillat
M
M
Maume
B F
BF
D001329:Autolysis; D008401:Gas Chromatography-Mass Spectrometry; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013261:Sterols / Q000737:chemistry*
D013261:Sterols