title: Expression of secretory leukocyte protease inhibitor in women with endometriosis.
abstract: OBJECTIVE
  To explore endometriosis-related molecules in patients with use of differential display analysis.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Nagoya City University Medical School, Nagoya, Japan.
  
  
  METHODS
  Women with endometriosis (n = 27) and without endometriosis (n = 21).
  
  
  METHODS
  Surgery was scheduled in the proliferative or secretory phase of the menstrual cycle.
  
  
  METHODS
  Differentially expressed products of endometrioma samples were sequenced at nucleotides. One of the candidate genes, secretory leukocyte protease inhibitor (SLPI) gene, was analyzed with use of in situ hybridization and Northern blot analyses. Distribution of SLPI was determined by immunohistochemistry, and the amount of SLPI in the peritoneal fluid and serum was measured by ELISA.
  
  
  RESULTS
  Distinct expression of SLPI messenger RNA could be detected in the endometrial-type epithelium of extrauterine endometriotic tissues and in the eutopic endometrium of women with endometriosis. SLPI was localized in the endometrial-type epithelium of endometriomas immunohistochemically. The amount of SLPI in the peritoneal fluid was markedly elevated in the endometriosis group (91.6+/-6.6 ng/mL compared with 68.4+/-5.3 ng/mL in the controls).
  
  
  CONCLUSIONS
  Secretory leukocyte protease inhibitor may be involved in the pathogenesis of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya City University Medical School, Nagoya, Japan. og.n.suz.@med.nagoya-cu.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Suzumori
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Yoneda
authors.forename: Y
authors.initials: Y
authors.lastname: Ozaki
authors.forename: H
authors.initials: H
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Suzumori
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism; D015152:Blotting, Northern; D016022:Case-Control Studies; D004715:Endometriosis / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008875:Middle Aged; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors / Q000235:genetics*
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors