Production and characterization of monoclonal antibodies to ARVCF.
We have generated the first monoclonal antibodies (MAbs) to Armadillo repeat gene deleted in velo-cardiofacial syndrome (ARVCF), a recently identified Armadillo repeat-containing protein closely related to the catenin p120ctn. Six ARVCF-specific MAbs were characterized for isotype, species cross-reactivity, and utility in assays including immunofluorescence, immunoprecipitation, and Western blotting. All six antibodies were isotyped as IgG1 and several cross-reacted with ARVCF from a variety of species including human, rat, dog, and monkey, but not mouse. Importantly, none of the ARVCF MAbs cross-reacted with p120ctn, despite the high homology between these proteins. MAbs 3B2 and 4B1 were consistently the best in all applications and will provide valuable tools for further study of the role of ARVCF in cells.
Mariner
D J
DJ
Department of Cell Biology, Vanderbilt University, Nashville, TN 37232, USA.
Sirotkin
H
H
Daniel
J M
JM
Lindman
B R
BR
Mernaugh
R L
RL
Patten
A K
AK
Thoreson
M A
MA
Reynolds
A B
AB
D000015:Abnormalities, Multiple / Q000235:genetics* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000917:Antibody Formation; D001135:Armadillos / Q000235:genetics* / Q000276:immunology*; D001665:Binding Sites; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D002460:Cell Line / Q000737:chemistry; D019465:Craniofacial Abnormalities / Q000235:genetics / Q000276:immunology; D004062:DiGeorge Syndrome / Q000235:genetics / Q000276:immunology; D004285:Dogs; D005455:Fluorescent Antibody Technique; D017353:Gene Deletion; D000882:Haplorhini; D006330:Heart Defects, Congenital / Q000235:genetics / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007365:Intercellular Junctions / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000276:immunology* / Q000378:metabolism*; D011233:Precipitin Tests; D051381:Rats; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014681:Velopharyngeal Insufficiency / Q000235:genetics / Q000276:immunology; D000095002:Delta Catenin
D000911:Antibodies, Monoclonal; D015820:Cadherins; D051177:Catenins; D015815:Cell Adhesion Molecules; D010750:Phosphoproteins; D000095002:Delta Catenin