Complement haemolytic activity (classical and alternative pathways), C3, C4 and factor B titres in healthy children.
Values of complement lytic activity of classical and alternative pathways, assessed by measuring the time required to lyse 50% of target red blood cells, and the concentration of complement components C3, C4 and factor B were estimated in the sera of 103 healthy children aged 3 to 14 y. Age-dependent variations were seen in the C3 and factor B concentrations, but not in C4, with the highest values found among 5-6-y-old children. Variations in classical and alternative lytic activity were not detected in this group of children, although the values are significantly different from our previously published data on adults, using the same kinetic assay (1). We also evaluated the relationship between the lytic activity of the classical (CPT) and alternative pathways (APT) and the levels of complement components. There were significant correlations between: APT and factor B, APT and C3, C3 and C4, C3 and factor B, and C4 and factor B concentrations. The normal ranges measured here can be used in the initial screening of Brazilian children presenting diseases involving the complement system. This study also contributes to a better understanding of the complement system ontogeny.
Ferriani
V P
VP
Department of Paediatrics, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil. vplferri@fmrp.usp.br
Barbosa
J E
JE
de Carvalho
I F
IF
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000704:Analysis of Variance; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D015932:Complement C3c / Q000032:analysis*; D003181:Complement C4 / Q000032:analysis*; D011415:Complement Factor B / Q000032:analysis*; D015941:Complement Hemolytic Activity Assay; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D003181:Complement C4; D015932:Complement C3c; D011415:Complement Factor B