Tandem repeat structure of rhamnose-binding lectin from catfish (Silurus asotus) eggs.
The primary structure of catfish (Silurus asotus) egg lectin (SAL) was determined. SAL cDNA contained 1448-bp nucleotides and 308 amino acid residues, deduced from open reading frame. The SAL mature protein composed of 285-amino acid residues was followed by a predicted signal sequence having 23 residues. The mRNA of SAL was found to be expressed in eggs, but not in liver. SAL is composed of three tandem repeat domain structures divided into exactly 95 amino acid residues each, and all cysteine positions of each domain were completely conserved. Sequence homologies between the three domains, termed D1 (1-95), D2 (96-190) and D3 (191-285), were as follows; D1-D2, 28%; D2-D3, 33%; D1-D3, 43%. Two conserved peptide motifs, -(AN)YGR(TD)S(T)XCS(TGR)P- and -DPCX(G)T(Y)KY(L)-, appear to exist at the N- and C-terminal regions of each domain, respectively. The kinetic parameters of SAL obtained by measuring surface plasmon resonance were as follows: K(a) (M(-1)) for neohesperidosyl-BSA, 7. 1 x 10(6); for melibiosyl-BSA, 4.9 x 10(6); and for lactosyl-BSA, 5. 2 x 10(5). These results show that RBLs including SAL comprise a family of alpha-galactosyl binding lectins having characteristic tandem repeat domain structures.
Hosono
M
M
Cancer Research Institute, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Japan.
Ishikawa
K
K
Mineki
R
R
Murayama
K
K
Numata
C
C
Ogawa
Y
Y
Takayanagi
Y
Y
Nitta
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000196:embryology / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D029941:Fish Proteins; D006023:Glycoproteins / Q000737:chemistry; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D020080:Tandem Repeat Sequences
D018076:DNA, Complementary; D029941:Fish Proteins; D006023:Glycoproteins; D037102:Lectins; D012333:RNA, Messenger; C402295:rhamnose-binding lectin, catfish