In vitro interactions of Caenorhabditis elegans dystrophin with dystrobrevin and syntrophin.
Dystrophin, the product of the gene mutated in Duchenne muscular dystrophy (DMD) is bound by its C-terminus to a protein complex including the related protein dystrobrevin. Both proteins contain a putative coiled-coil domain consisting of two alpha-helices. It has been reported that the two proteins bind to each other by the first one of the two alpha-helices. We have revisited this question using the Caenorhabditis elegans homologs of dystrophin and dystrobrevin. In vitro interaction occurs through the more conserved second helix. We propose a new model of dystrophin interactions with associated proteins.
Gieseler
K
K
IMPC, CNRS-UPR411, 660 route des lucioles, 06560, Sophia Antipolis, France.
Abdel-Dayem
M
M
Ségalat
L
L
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry*; D029742:Caenorhabditis elegans Proteins; D016189:Dystrophin / Q000737:chemistry / Q000378:metabolism*; D049029:Dystrophin-Associated Proteins; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; C490915:DTNA protein, human; C479075:Dyb-1 protein, C elegans; D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011993:Recombinant Fusion Proteins; C091824:syntrophin; D005982:Glutathione Transferase