Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome.
The nucleotide sequence of the DNA genome of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV), a group II NPV, was determined and analysed. The genome contains 135611 bp and has a G+C content of 44 mol%. Computer-assisted analysis revealed 139 ORFs of 150 nucleotides or larger; 103 have homologues in Autographa californica MNPV (AcMNPV) and a further 16 have homologues in other baculoviruses. Twenty ORFs are unique to SeMNPV. Major differences in SeMNPV gene content and arrangement were found compared with the group I NPVs AcMNPV, Bombyx mori (Bm) NPV and Orgyia pseudotsugata (Op) MNPV and the group II NPV Lymantria dispar (Ld) MNPV. Eighty-five ORFs were conserved among all five baculoviruses and are considered as candidate core baculovirus genes. Two putative p26 and odv-e66 homologues were identified in SeMNPV, each of which appeared to have been acquired independently and not by gene duplication. The SeMNPV genome lacks homologues of the major budded virus glycoprotein gene gp64, the immediate-early transactivator ie-2 and bro (baculovirus repeat ORF) genes that are found in AcMNPV, BmNPV, OpMNPV and LdMNPV. Gene parity analysis of baculovirus genomes suggests that SeMNPV and LdMNPV have a recent common ancestor and that they are more distantly related to the group I baculoviruses AcMNPV, BmNPV and OpMNPV. The orientation of the SeMNPV genome is reversed compared with the genomes of AcMNPV, BmNPV, OpMNPV and LdMNPV. However, the gene order in the 'central' part of baculovirus genomes is highly conserved and appears to be a key feature in the alignment of baculovirus genomes.
IJkel
Wilfred F J
WFJ
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
van Strien
Elisabeth A
EA
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Heldens
Jacobus G M
JGM
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Broer
René
R
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Zuidema
Douwe
D
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Goldbach
Rob W
RW
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Vlak
Just M
JM
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002213:Capsid / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D018411:Spodoptera / Q000821:virology*; D015678:Viral Structural Proteins / Q000235:genetics
D015678:Viral Structural Proteins