Time-resolved fluoroimmunoassay for delta(9)-tetrahydrocannabinol as applied to early discrimination of Cannabis sativa plants.
In the cultivations of Cannabis, it is important to be able to distinguish fiber-type plants from drug-type plants by an easy observation of their phenotype. This study required the screening of many samples for their cannabinoid content. A simple and highly sensitive time-resolved fluoroimmunological method was developed for the determination of Delta(9)-tetrahydrocannabinol in the leaf extracts. The useful range of the calibration curve was between 10 pg and 25 ng of standard. Matrix effects were minimized by a high dilution of samples.
Bacigalupo
M A
MA
Istituto di Biocatalisi e Riconoscimento Molecolare, CNR, Via Mario Bianco 9, Milano 20131, Italy. bacigalupo@ico.mi.cnr.it
Ius
A
A
Meroni
G
G
Grassi
G
G
Moschella
A
A
D000818:Animals; D002188:Cannabis / Q000145:classification*; D002849:Chromatography, Gas; D013759:Dronabinol / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C
D013759:Dronabinol