Selective hydrolysis of nucleotides to nucleosides and free bases.
The kinetics of the hydrolysis of 2'-deoxyadenosine-5'-monophosphoric acid (dAMP), 2'-deoxycytidine-5'-monophosphoric acid (dCMP), 2'-deoxyguanosine-5'-monophosphoric acid (dGMP) and tymidine-5'-monophosphoric acid (dTMP) was studied in the presence of Xanthomonas maltophilia [1]. The reaction products are nucleosides: 2'-deoxyadenosine (dA), 2'-deoxycytidine (dC), 2'-deoxyguanosine (dG) and tymidine (dT), respectively, or the respective free bases. Hydrolysis of dTMP and dGMP proceeded stepwise according to the sequence: nucleotide-->nucleoside-->free base, whereas no accumulation of the free base was observed during the hydrolysis of dAMP and dCMP.
Chmielowiec
U
U
Pharmaceutical Research Institute, Department of Chemistry, Warsaw, Poland. hanka@stratus.waw.pl
Kruszewska
H
H
Cybulski
J
J
D006868:Hydrolysis; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D014974:Xanthomonas / Q000378:metabolism
D009705:Nucleosides; D009711:Nucleotides