title: Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa.
abstract: Cystic fibrosis (CF) patients develop chronic airway infections with Pseudomonas aeruginosa (PA). Pseudomonas aeruginosa synthesized lipopolysaccharide (LPS) with a variety of penta- and hexa-acylated lipid A structures under different environmental conditions. CF patient PA synthesized LPS with specific lipid A structures indicating unique recognition of the CF airway environment. CF-specific lipid A forms containing palmitate and aminoarabinose were associated with resistance to cationic antimicrobial peptides and increased inflammatory responses, indicating that they are likely to be involved in airway disease.
authors.affiliation: Department of Medicine, University of Washington, Seattle, WA 98195, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Ernst
authors.forename: E C
authors.initials: EC
authors.lastname: Yi
authors.forename: L
authors.initials: L
authors.lastname: Guo
authors.forename: K B
authors.initials: KB
authors.lastname: Lim
authors.forename: J L
authors.initials: JL
authors.lastname: Burns
authors.forename: M
authors.initials: M
authors.lastname: Hackett
authors.forename: S I
authors.initials: SI
authors.lastname: Miller
mesh_terms: D000215:Acylation; D023181:Antimicrobial Cationic Peptides; D001089:Arabinose / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D006801:Humans; D007223:Infant; D016209:Interleukin-8 / Q000096:biosynthesis; D008050:Lipid A / Q000096:biosynthesis* / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology; D008274:Magnesium / Q000494:pharmacology; D009154:Mutation; D010168:Palmitates / Q000032:analysis / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011113:Polymyxins / Q000494:pharmacology; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D012137:Respiratory System / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014774:Virulence
chemical_list: D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; C081140:C18G peptide; D016209:Interleukin-8; D008050:Lipid A; D008070:Lipopolysaccharides; D010168:Palmitates; D010455:Peptides; C079792:PhoQ protein, Bacteria; D011113:Polymyxins; C016982:aminoarabinose; C061064:PhoP protein, Bacteria; D001089:Arabinose; D008274:Magnesium