The first triple gene block protein of peanut clump virus localizes to the plasmodesmata during virus infection.
The subcellular localization of the first triple gene block protein (TGBp1) of peanut clump pecluvirus (PCV) was studied by subcellular fractionation and immunogold cytochemistry using TGBp1-specific antibodies raised against a fusion protein expressed in and purified from bacteria. In the inoculated and apical leaves of virus-infected Nicotiana benthamiana, TGBp1 localized to the cell wall and P30 fractions. Electron microscopy of immunogold-decorated ultrathin sections of the infected leaf tissue revealed TGBp1-specific labeling of the plasmodesmata joining mesophyll cells. In longitudinal sections of the plasmodesmata, the TGBp1-specific labeling was most commonly associated with the plasmodesmal collar region. In transgenic N. benthamiana, which constitutively expressed TGBp1, no TGBp1-specific immunogold labeling of plasmodesmata was observed, but plasmodesmata were gold decorated when the transgenic plants were infected with a TGBp1-defective PCV mutant, indicating that factors induced by the virus infection target and/or anchor the transgene TGBp1 to the plasmodesmata.
Erhardt
M
M
Institut de Biologie Moléculaire des Plantes, CNRS, et de, l'Université Louis Pasteur, 12 rue du Général Zimmer, Strasbourg Cedex, 67084, France.
Stussi-Garaud
C
C
Guilley
H
H
Richards
K E
KE
Jonard
G
G
Bouzoubaa
S
S
D010367:Arachis / Q000821:virology*; D001483:Base Sequence; D017931:DNA Primers; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D013347:Subcellular Fractions / Q000821:virology; D014026:Nicotiana / Q000821:virology
D017931:DNA Primers; D011994:Recombinant Proteins