NADPH-diaphorase-containing neurons in cortex, subcortical white matter and neostriatum are selectively spared in Alzheimer's disease.
To investigate the involvement of NADPH-diaphorase (NADPH-d)-containing neurons in Alzheimer's disease (AD), NADPH-d enzyme histochemistry in vibratome sections was applied to the superior frontal and superior temporal cortex and the neostriatum in 5 AD and 6 aged control brains. Overall there was a neuronal loss and atrophy in the cortex of AD. Despite slight morphological neuronal changes in the cortex of AD, we found no significant difference in the number of NADPH-d-positive neurons in both cortex and neostriatum between control and AD cases. These results provide further evidence for a selective preservation of NADPH-d neurons in AD. In order to check whether nNOS-immunoreactive neurons are identical to NADPH-d-positive neurons in the human brain, we examined the frontal and temporal cortex and neostriatum of normal human brains in serial cryostat sections. We found that nNOS-containing neurons paralleled NADPH-d-positive neurons in these brain regions. Copyrightz1999S.KargerAG,Basel
Tao
Z
Z
Department of Neuropathology, University Hospital Gasthuisberg, Leuven, Belgium.
Van Gool
D
D
Lammens
M
M
Dom
R
R
D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology*; D001921:Brain / Q000201:enzymology* / Q000473:pathology*; D002452:Cell Count; D016764:Cell Polarity / Q000502:physiology; D002540:Cerebral Cortex / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D005625:Frontal Lobe / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007395:Interneurons / Q000201:enzymology / Q000473:pathology; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D017072:Neostriatum / Q000201:enzymology* / Q000473:pathology*; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D013702:Temporal Lobe / Q000201:enzymology / Q000473:pathology
C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D009252:NADPH Dehydrogenase