Growth factors stimulate the activity of key glycolytic enzymes in isolated digestive gland cells from mussels (Mytilus galloprovincialis Lam.) through tyrosine kinase mediated signal transduction.
Digestive gland cells isolated from mussels (Mytilus) have previously been demonstrated to respond to mammalian EGF with a cytosolic Ca(2+) transient and stimulated DNA synthesis; both responses were mediated by activation of tyrosine kinase receptors. The present study examines the mechanisms involved in further signal progression and possible targets of phosphorylation/dephosphorylation processes. The effects of EGF, IGF-I, and insulin on the activity of two key glycolytic enzymes PFK (phosphofructokinase) and PK (pyruvate kinase) were evaluated. All the peptides tested induced a transient and dose-dependent stimulation of the activity of both PFK and PK, which involved activation of MAPKs. Quantitative immunoelectron microscopy, utilizing monoclonal anti-phosphotyrosine antibodies, revealed that EGF induced a transient increase in tyrosine phosphorylation. The results demonstrate that, in marine invertebrate cells, activation of tyrosine kinase membrane receptors by growth factors triggers signal transduction pathways involving a phosphorylative cascade similar to that of mammalian cells. Moreover, these data suggest that, in mussel cells, growth factors may play a physiological role in the in vivo regulation of glucose metabolism by modulating, through reversible phosphorylation, the activity of key glycolytic enzymes.
Canesi
L
L
Istituto di Scienze Fisiologiche, Universit¿a di Urbino, Loc. Crocicchia.
Ciacci
C
C
Betti
M
M
Malatesta
M
M
Gazzanelli
G
G
Gallo
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D049872:Bivalvia / Q000201:enzymology*; D004064:Digestive System / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D016253:Microscopy, Immunoelectron; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000276:immunology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D007328:Insulin; D019000:Phosphotyrosine; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D011505:Protein-Tyrosine Kinases