title: Activation of NF-kappaB in human osteoblasts by stimulators of bone resorption.
abstract: Several bone resorptive stimuli affect osteoclasts indirectly by modulating the production and release of osteoblastic factors. Using electrophoretic mobility shift assays, we found that not only tumour necrosis factor-alpha (TNF-alpha) but also interleukin-1beta and parathyroid hormone (PTH) caused dose and time-related increases in nuclear factor kappaB (NF-kappaB)-DNA binding in Saos-2 human osteoblastic (hOB) cells. Activation of NF-kappaB by TNF-alpha was reproduced in primary hOBs. In contrast, consistent with their previously reported lack of response to steroid hormones, Saos-2 cells did not respond to 1,25-dihydroxyvitamin D(3). We suggest that NF-kappaB activation in osteoblastic cells constitutes an important pathway in osteoblast-mediated resorptive signalling.
authors.affiliation: Bone and Joint Research Unit, St. Bartholomew's and the Royal London School of Medicine and Dentistry, 25-29 Ashfield Street, London, UK. nadire@ahmet17.freeserve.co.uk
authors.forename: N N
authors.initials: NN
authors.lastname: Ali
authors.forename: V
authors.initials: V
authors.lastname: Gilston
authors.forename: P G
authors.initials: PG
authors.lastname: Winyard
mesh_terms: D000328:Adult; D001862:Bone Resorption; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism* / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014807:Vitamin D / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D016328:NF-kappa B; D009687:Nuclear Proteins; D010281:Parathyroid Hormone; D014409:Tumor Necrosis Factor-alpha; D014807:Vitamin D; C097949:1,25-dihydroxyvitamin D