Nrk: a murine X-linked NIK (Nck-interacting kinase)-related kinase gene expressed in skeletal muscle.
We report the cloning and expression pattern of a novel Ste20-type kinase gene, NIK-related kinase (Nrk), located on the mouse X chromosome. The full-length Nrk cDNA encodes a 1455-amino-acid polypeptide characterized by a N-terminal Ste20-type catalytic domain and a C-terminal regulatory domain characteristic of the group I GCK subfamily. The overall structure of the NRK protein is closely related to that of Nck-interacting kinase (Nik). In situ hybridization revealed that Nrk was predominantly expressed in skeletal muscle during mouse embryogenesis. Nrk gene expression was detected in the myotome at 10.5 dpc and, thereafter, was observed in developing skeletal musculature from 11.5 to 13.5 dpc. However, expression in skeletal muscle was not observed in adults.
Kanai-Azuma
M
M
Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, Japan.
Kanai
Y
Y
Okamoto
M
M
Hayashi
Y
Y
Yonekawa
H
H
Yazaki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D020134:Catalytic Domain; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D047908:Intracellular Signaling Peptides and Proteins; D018878:Limb Buds; D020930:MAP Kinase Kinase Kinases; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000196:embryology* / Q000201:enzymology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014960:X Chromosome
D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; C411848:Nik related kinase; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C078754:STE20 protein, S cerevisiae