Characterization of a biosurfactant, mannosylerythritol lipid produced from Candida sp. SY16.
One yeast strain, SY16, was selected as a potential producer of a biosurfactant, and identified as a Candida species. A biosurfactant produced from Candida sp. SY16 was purified and confirmed to be a glycolipid. This glycolipid-type biosurfactant lowered the surface tension of water to 29 dyne/cm at critical micelle concentration of 10 mg/l (1.5 x 10(-5) M), and the minimum interfacial tension was 0.1 dyne/cm against kerosene. Thin-layer and high-pressure liquid chromatography studies demonstrated that the glycolipid contained mannosylerythritol as a hydrophilic moiety. The hydrophilic sugar moiety of the biosurfactant was determined to be beta-D-mannopyranosyl-(1-->4)-O-meso-erythritol by nuclear magnetic resonance (NMR) and fast atom bombardment mass-spectroscopy analyses. The hydrophobic moiety, fatty acids, of the biosurfactant was determined to be hexanoic, dodecanoic, tetradecanoic, and tetradecenoic acid by gas chromatography-mass spectroscopy. The structure of the native biosurfactant was determined to be 6-O-acetyl-2,3-di-O-alkanoyl-beta-D-mannopyranosyl-(1-->4)-O-meso- erythritol by NMR analyses. We newly determined that an acetyl group was linked to the C-6 position of the D-mannose unit in the hydrophilic sugar moiety.
Kim
H S
HS
Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
Yoon
B D
BD
Choung
D H
DH
Oh
H M
HM
Katsuragi
T
T
Tani
Y
Y
D002175:Candida / Q000302:isolation & purification / Q000378:metabolism*; D002208:Caproates / Q000032:analysis; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004896:Erythritol / Q000031:analogs & derivatives* / Q000032:analysis; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D007850:Lauric Acids / Q000032:analysis; D008358:Mannose / Q000032:analysis; D013058:Mass Spectrometry; D019814:Myristic Acid / Q000032:analysis; D012988:Soil Microbiology; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism
D002208:Caproates; D006017:Glycolipids; D007850:Lauric Acids; D013501:Surface-Active Agents; D019814:Myristic Acid; C030358:lauric acid; C037652:hexanoic acid; D008358:Mannose; D004896:Erythritol