title: Partitioning of selected antioxidants in mayonnaise.
abstract: This study examined partitioning of alpha-, beta-, and gamma-tocopherol and six polar antioxidants (Trolox, ferulic acid, caffeic acid, propyl gallate, gallic acid, and catechin) in mayonnaise. Partitioning of antioxidants between different phases was determined after separation of mayonnaise by either (a) centrifugation + ultracentrifugation or (b) centrifugation + dialysis. Antioxidants partitioned in accordance with their chemical structure and polarity: Tocopherols were concentrated in the oil phase (93-96%), while the proportion of polar antioxidants in the oil phase ranged from 0% (gallic acid and catechin) to 83% (Trolox). Accordingly, proportions of 6% (Trolox) to 80% (gallic acid and catechin) were found in the aqueous phase. Similar trends were observed after dialysis. After ultracentrifugation, large proportions of polar antioxidants were found in the "emulsion phase" and the "precipitate" (7-34% and 2-7%, respectively). This indicated entrapment of antioxidants at the oil-water interface in mayonnaise. The results signify that antioxidants partitioning into different phases of real food emulsions may vary widely.
authors.affiliation: Danish Institute for Fisheries Research, Department of Seafood Research, Building 221, Technical University of Denmark, DK-2800 Lyngby, Denmark. cja@dfu.min.dk
authors.forename: C
authors.initials: C
authors.lastname: Jacobsen
authors.forename: K
authors.initials: K
authors.lastname: Schwarz
authors.forename: H
authors.initials: H
authors.lastname: Stöckmann
authors.forename: A S
authors.initials: AS
authors.lastname: Meyer
authors.forename: J
authors.initials: J
authors.lastname: Adler-Nissen
mesh_terms: D019342:Acetic Acid; D000975:Antioxidants / Q000032:analysis*; D002109:Caffeic Acids / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002839:Chromans / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002957:Citrus; D003296:Cooking; D003373:Coumaric Acids; D004530:Egg Yolk; D005229:Fatty Acids, Monounsaturated; D005395:Fish Oils; D005504:Food Analysis; D005707:Gallic Acid / Q000032:analysis; D010938:Plant Oils; D011435:Propyl Gallate / Q000032:analysis; D000074262:Rapeseed Oil; D014810:Vitamin E / Q000032:analysis*
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D002839:Chromans; D003373:Coumaric Acids; D005229:Fatty Acids, Monounsaturated; D005395:Fish Oils; D010938:Plant Oils; D000074262:Rapeseed Oil; D014810:Vitamin E; D005707:Gallic Acid; D011435:Propyl Gallate; D002392:Catechin; C004999:ferulic acid; D019342:Acetic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; C040048:caffeic acid