Distribution of the cfiA gene among Bacteroides fragilis strains in Japan and relatedness of cfiA to imipenem resistance.
The cfiA gene, encoding an imipenem-hydrolyzing metallo-beta-lactamase produced by Bacteroides fragilis, and insertion-like elements were detected by PCR amplification with B. fragilis strains isolated in Japan. The cfiA gene was found in 1.9 and 4.1% of the imipenem-susceptible B. fragilis isolates collected from 1987 to 1988 and from 1992 to 1994, respectively. Insertion-like elements adjacent to the cfiA gene were found in all nine metallo-beta-lactamase-producing imipenem-resistant strains tested but not in nine cfiA-positive strains with no detectable metallo-beta-lactamase activity.
Yamazoe
K
K
Institute of Anaerobic Bacteriology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan.
Kato
N
N
Kato
H
H
Tanaka
K
K
Katagiri
Y
Y
Watanabe
K
K
D001426:Bacterial Proteins; D001441:Bacteroides fragilis / Q000187:drug effects* / Q000235:genetics*; D004352:Drug Resistance, Microbial; D015378:Imipenem / Q000494:pharmacology*; D015345:Oligonucleotide Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase