Complete nucleotide sequence and molecular characterization of hemolysin II gene from Bacillus cereus.
Hemolysin II gene from Bacillus cereus VKM-B771 has been sequenced. The deduced primary translation product consists of 412 amino acid residues which corresponds to the protein with an M(r) of 45.6 kDa. The predicted mature Hly-II protein (residues 32 to 412) is of 42.3 kDa, which is in close agreement with the mini-cell electrophoresis analysis. Hly-II deletion variant lacking 96 C-terminal residues still has hemolytic activity. The protein primary structure analysis revealed no homology with any known Bacillus cytolysins. Significant general homology (31-28% identity) was found between the hemolysin II and Staphylococcus aureus alpha-toxin, gamma-hemolysin (HlgB), and leukocidins (LukF, LukF-R, LukF-PV). The data suggest that hemolysin II belongs to the group of beta-channel forming cytolysins.
Baida
G
G
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow region, 142292, Pushchino, Russia. gbaida@uic.edu
Budarina
Z I
ZI
Kuzmin
N P
NP
Solonin
A S
AS
D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D007956:Leukocidins / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics; D010738:Type C Phospholipases / Q000235:genetics
C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D007956:Leukocidins; D010738:Type C Phospholipases