Nonisotopic detection of Loma salmonae (microspora) in rainbow trout (Oncorhynchus mykiss) gills by in situ hybridization.
Loma salmonae, a microsporidian parasite of salmonids of the genus Oncorhynchus, is a significant cause of economic loss in pen-reared chinook salmon (O. tschawytscha). Final stages of L. salmonae infections are easily recognized by the xenomas that form in the gills during sporogony. However, early prexenoma stages of infection (3 weeks or less after infection) are difficult to detect on histologic slides. An L. salmonae-specific single-stranded DNA probe labeled with digoxigenin was used to detect these prexenoma stages of L salmonae by in situ hybridization in experimentally infected rainbow trout. This method allows detection of the parasite in the gills only 2 weeks after infection, providing a sensitive and specific way of detecting L. salmonae during the early stages of infection.
Sanchez
J G
JG
Speare
D J
DJ
Markham
R J
RJ
D000818:Animals; D015342:DNA Probes / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004076:Digoxigenin / Q000737:chemistry; D005393:Fish Diseases / Q000469:parasitology* / Q000473:pathology; D005880:Gills / Q000469:parasitology* / Q000473:pathology; D017403:In Situ Hybridization / Q000662:veterinary; D016816:Microsporida / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D017686:Oncorhynchus mykiss / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity
D015342:DNA Probes; D016054:DNA, Protozoan; D004277:DNA, Single-Stranded; D004076:Digoxigenin