title: Dependence of echovirus 9 on the enterovirus RNA replication inhibitor 2-(alpha-Hydroxybenzyl)-benzimidazole maps to nonstructural protein 2C.
abstract: HBB [2-(alpha-hydroxybenzyl)-benzimidazole] selectively inhibits RNA synthesis of most enteroviruses. However, isolation of HBB-dependent variants is possible. Sequence analysis and characterization of recombinant viruses revealed that HBB dependence maps to the nonstructural protein 2C. A single point mutation at position C(4782)U is sufficient to establish the HBB-dependent phenotype in our echovirus 9 model.
authors.affiliation: Institut für Virologie der Universität zu Köln, 50935 Cologne, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Hadaschik
authors.forename: M
authors.initials: M
authors.lastname: Klein
authors.forename: H
authors.initials: H
authors.lastname: Zimmermann
authors.forename: H J
authors.initials: HJ
authors.lastname: Eggers
authors.forename: B
authors.initials: B
authors.lastname: Nelsen-Salz
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D003001:Cloning, Molecular; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D006801:Humans; D017354:Point Mutation; D020365:RNA Helicases / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins
chemical_list: D000998:Antiviral Agents; D001562:Benzimidazoles; D012367:RNA, Viral; D014764:Viral Proteins; C021033:HBBPC; C116598:2C helicase; D020365:RNA Helicases