title: Cloning and comparative protein modeling of two purple acid phosphatase isozymes from sweet potatoes (Ipomoea batatas).
abstract: The sequence of cDNA fragments of two isozymes of the purple acid phosphatase from sweet potato (spPAP1 and spPAP2) has been determined by 5' and 3' rapid amplification of cDNA ends protocols using oligonucleotide primers based on amino acid information. The encoded amino acid sequences of these two isozymes show an equidistance of 72-77% not only to each other, but also to the primary structure of the purple acid phosphatase from red kidney bean (kbPAP). A three-dimensional model of the active site has been constructed for spPAP2 on the basis of the kbPAP crystallographic structure that helps to explain the reported differences in the visible and EPR spectra of spPAP2 and kbPAP.
authors.affiliation: Anorganisch-Chemisches Institut, Universität Münster, Wilhelm-Klemm-Strasse 8, 48149, Münster, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Durmus
authors.forename: C
authors.initials: C
authors.lastname: Eicken
authors.forename: F
authors.initials: F
authors.lastname: Spener
authors.forename: B
authors.initials: B
authors.lastname: Krebs
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D007527:Isoenzymes; D010940:Plant Proteins; D012313:RNA; C091137:purple acid phosphatase; D000135:Acid Phosphatase