title: Rapid identification of Burkholderia pseudomallei in blood cultures by latex agglutination using lipopolysaccharide-specific monoclonal antibody.
abstract: Melioidosis, an infection caused by Burkholderia pseudomallei, is endemic in Southeast Asia. The septicemic form of melioidosis is the leading cause of death due to community-acquired bacteremia in the northeastern part of Thailand. The delay in isolation and identification of the causative organism is a major contributing factor to the high mortality. The present study describes the evaluation of a latex agglutination test for rapid identification of the bacteria directly from blood cultures. The Bps-L1 monoclonal antibody recognized the lipopolysaccharide antigen of 96.8% of B. pseudomallei clinical isolates and was highly specific for B. pseudomallei. The diagnostic value of the latex agglutination test based on Bps-L1 monoclonal antibody was prospectively evaluated in an area endemic for melioidosis. The agglutination test kit was evaluated in 88 blood cultures with gram-negative bacteria identified with Gram staining. The sensitivity and specificity of the test kit were both 100%. These results indicated that the detection of B. pseudomallei lipopolysaccharide by specific monoclonal antibody in a latex agglutination format is clinically useful for the rapid identification of the bacteria in blood cultures in areas endemic for melioidosis.
authors.affiliation: Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Dharakul
authors.forename: S
authors.initials: S
authors.lastname: Songsivilai
authors.forename: S
authors.initials: S
authors.lastname: Smithikarn
authors.forename: C
authors.initials: C
authors.lastname: Thepthai
authors.forename: A
authors.initials: A
authors.lastname: Leelaporn
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000097:blood*; D016470:Bacteremia / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D015153:Blotting, Western; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D008070:Lipopolysaccharides / Q000276:immunology; D008554:Melioidosis / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D016622:Silver Staining; D013785:Thailand
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides