cDNA cloning and chromosomal mapping of mouse BH-protocadherin.
We isolated and determined the sequence of cDNA encoding mouse BH-protocadherin (BH-Pcdh) from heart. It encodes a 1069 amino acids (aa) polypeptide exhibiting an overall 97% identity with human BH-Pcdh-a and 83% identity with Xenopus NF-protocadherin. We also determined the alternatively spliced cytoplasmic tail sequence. The cytoplasmic tail of mouse BH-Pcdh-b is short (2 aa) compared with that of human BH-Pcdh-b (14 aa). The cytoplasmic tail of mouse BH-Pcdh-c showed 100% aa identity with that of human BH-Pcdh-c except for a 8-amino-acid insertion. Northern blot analysis revealed two major transcripts were expressed in brain and heart. Mouse BH-Pcdh-a mRNA was detected as a single band of approximately 5.5-kb. The mRNA level of mouse BH-Pcdh-a and -c were persistently detected by RT-PCR in developmental process of brain and heart, but that of mouse BH-Pcdh-b was elevated only in fetus and neonatal stage of brain. The chromosomal location of the mouse BH-Pcdh gene was determined as 5C3-D using fluorescence in situ hybridization.
Yoshida
K
K
Department of Virology, Institute of Medical Science, University of Tokyo, Japan.
Hida
M
M
Watanabe
M
M
Yamaguchi
R
R
Tateyama
S
S
Sugano
S
S
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000502:physiology; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000502:physiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D000091363:Protocadherins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D015820:Cadherins; C113391:PCDH7 protein, human; D020033:Protein Isoforms; D000091363:Protocadherins