title: Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens britton.
abstract: Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS-PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO(4) oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC(50) = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants.
authors.affiliation: Cell Biology Laboratory, Biosignalling Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Asada
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumori
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Nakagomi
authors.forename: M
authors.initials: M
authors.lastname: Sugie
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: N
authors.initials: N
authors.lastname: Tomizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: S
authors.initials: S
authors.lastname: Oka
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D019686:Lamiaceae / Q000737:chemistry*; D008407:Mast Cells / Q000187:drug effects / Q000648:ultrastructure; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D006023:Glycoproteins; D010936:Plant Extracts; D011493:Protein Kinase C; D006821:Hyaluronoglucosaminidase