Identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides) by PCR amplification of the 5S rDNA gene.
Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene, has been used for the identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides). Two species-specific primers were designed to amplify specific fragments of the 5S rDNA gene in each species. The remarkably different size of the amplicons obtained gives, by simple agarose gel electrophoresis, two distinguishable band patterns for both flatfish species. This genetic marker can be very useful for the accurate identification of S. solea and Greenland halibut, to enforce labeling regulations.
Céspedes
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Departamento de Nutrición y Bromatología III, Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. anaces@eucmax.sim.ucm.es
García
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T
Carrera
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E
González
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I
Fernández
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A
Hernández
P E
PE
Martín
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R
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D017747:Seafood / Q000145:classification*; D013045:Species Specificity
D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S