Thermodynamics of single mismatches in RNA duplexes.
The thermodynamic properties and structures of single mismatches in short RNA duplexes were studied in optical melting and imino proton NMR experiments. The free energy increments at 37 degrees C measured for non-GU single mismatches range from -2.6 to 1.7 kcal/mol. These increments depend on the identity of the mismatch, adjacent base pairs, and the position in the helix. UU and AA mismatches are more stable close to a helix end, but GG mismatch stability is essentially unaffected by the position in the helix. Approximations are suggested for predicting stabilities of single mismatches in short RNA duplexes.
Kierzek
R
R
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Noskowskiego.
Burkard
M E
ME
Turner
D H
DH
D000225:Adenine / Q000737:chemistry; D020137:Base Pair Mismatch; D020029:Base Pairing; D001483:Base Sequence; D006147:Guanine / Q000737:chemistry; D006358:Hot Temperature; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000737:chemistry; D013816:Thermodynamics; D014498:Uracil / Q000737:chemistry
D009692:Nucleic Acid Heteroduplexes; D012335:RNA, Ribosomal; D014498:Uracil; D006147:Guanine; D012313:RNA; D000225:Adenine