Characterization of subclonal cell lines showing high motility derived from an undifferentiated human endometrial cancer cell line.
To examine the relationship between the morphology and the motility of cancer cells, we studied the difference between human endometrial undifferentiated cancer cell lines with different motilities, using light and electron microscopy, immunocytochemistry and RT-PCR. In a transplanted tumor of a cell line with high motility, many connective tissues and microvessels were observed and there were few intercellular spaces, which were ultrastructurally full of extracellular matrix. In the high-motility cell line only cytokeratin 19 was observed, and the level of cytokeratin 19 mRNA was elevated. Thus, motility is suggested to be related to the induction of connective tissues and cytoskeleton.
Koizumi
M
M
Department of Gynecology and Obstetrics, Sapporo Medical University, School of Medicine, Sapporo, Japan. koizumim@sapmed.ac.jp
Ito
E
E
Yamashita
S
S
Baba
T
T
Yamanaka
I
I
Suzuki
T
T
Kudo
R
R
D000818:Animals; D002465:Cell Movement; D016889:Endometrial Neoplasms / Q000737:chemistry / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D007633:Keratins