Nuclear F-actin is required for AcMNPV nucleocapsid morphogenesis.
During nucleocapsid assembly, filamentous actin (F-actin) colocalizes with the major capsid protein of Autographa californica M nucleopolyhedrovirus (AcMNPV) within nuclei of infected lepidopteran host cells. Cytochalasin D (CD) disrupts actin filaments and prevents assembly of progeny AcMNPV, suggesting that nuclear F-actin is essential for nucleocapsid morphogenesis. Direct proof for this hypothesis was provided by the demonstration that two AcMNPV recombinants engineered to express either wild-type- or CD-resistant actin at equivalent rates were differentially sensitive to CD. The AcMNPV requirement for nuclear F-actin is unique among intracellular pathogens and may constitute a significant host range factor.
Ohkawa
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Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102, USA.
Volkman
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LE
D000199:Actins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D015638:Cytochalasin D / Q000494:pharmacology; D007700:Kinetics; D007915:Lepidoptera / Q000821:virology; D009024:Morphogenesis; D019251:Nucleocapsid / Q000096:biosynthesis*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis
D000199:Actins; D011994:Recombinant Proteins; D015638:Cytochalasin D