title: Comet assay application in environmental monitoring: DNA damage in human leukocytes and plant cells in comparison with bacterial and yeast tests.
abstract: Urban airborne particulate is a complex mixture of air pollutants, many of which have not been identified. However, short-term mutagenesis tests together with chemicophysical parameter analysis are able to better assess air quality and genotoxic load. The findings of continuous monitoring (January 1991-August 1998) of urban air genotoxicity of a Po Valley town (Italy) on Salmonella typhimurium and Saccharomyces cerevisiae are reported. During this period, various measures (catalytic devices, unleaded fuels, annual vehicle overhaul, etc.) to improve air-dispersed pollutant control were enforced. However, a continuous presence of genotoxic compounds is shown and more qualitative than quantitative changes are evident. We also demonstrate the ability of the Comet assay to detect DNA-damaging agents in airborne particulate samples. We applied the test to human leukocytes and, with major improvements, to plant cells (Allium cepa roots and epigean tissues of Impatiens balsamina). The first findings on human leukocytes confirm the sensitivity of this assay, its peculiarity and its applicability in assessing genotoxicity in environmental samples. The capability of plants to show the response of multicellular organisms to environmental pollutants largely counterbalances a probable lowering in sensitivity. Moreover, application of the Comet test to epigean tissues could be useful in estimating the bioavailability of and genotoxic damage by air pollutants, including volatile compounds (ozone, benzene, nitrogen oxides, etc.) to higher plants.
authors.affiliation: Istituto di Genetica, Università degli Studi di Parma, Parco Area delle Scienze 11/a, 43100 Parma and ARPA Emilia Romagna, Sezione di Parma, Parma, Italy. mutgen@unipr.it
authors.forename: P
authors.initials: P
authors.lastname: Poli
authors.forename: A
authors.initials: A
authors.lastname: Buschini
authors.forename: F M
authors.initials: FM
authors.lastname: Restivo
authors.forename: A
authors.initials: A
authors.lastname: Ficarelli
authors.forename: F
authors.initials: F
authors.lastname: Cassoni
authors.forename: I
authors.initials: I
authors.lastname: Ferrero
authors.forename: C
authors.initials: C
authors.lastname: Rossi
mesh_terms: D002478:Cells, Cultured; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000187:drug effects*; D019697:Onions / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D018744:DNA, Plant; D004247:DNA