title: Racial differences in prostate cancer related to loss of heterozygosity on chromosome 8p12-23.
abstract: OBJECTIVE
  To determine if there is a racial difference in prostate cancer related to loss of heterozygosity (LOH) on chromosome 8p12-23, the region most frequently altered in prostate cancer.
  
  
  METHODS
  A total of 51 prostate cancer patients, consisting of 23 African Americans and 28 Caucasians, were included in this study. All patients underwent radical prostatectomy, and patients in the two racial subgroups were matched for median serum PSA, Gleason score, and pathological stage of cancer. Paired normal prostate and cancer tissue DNA was isolated and amplified with 13 polymorphic markers mapped to 8p12-23 by radiolabeled polymerase chain reaction. The amplified products were resolved by polyacrylamide gel electrophoresis, autoradiographed, and analyzed for allelic losses.
  
  
  RESULTS
  The overall incidence of LOH at 8p12-23 was 53%, and 16% showed homozygous deletions. The incidence of LOH in Caucasians was 68% compared to 35% in African Americans. On univariate (p = 0.02) and multivariate logistic regression analysis (p = 0.02), only Caucasian race was a significant predictor for LOH. The other clinicopathologic parameters did not have any significant effect on incidence of LOH.
  
  
  CONCLUSIONS
  These results highlight the independent influence of Caucasian race on incidence of LOH at 8p12-23, and suggest that genetic differences at specific tumor suppressor loci may be a factor responsible for racial variations observed in prostate cancer.
authors.affiliation: Department of Radiation Oncology, Northwestern Memorial Hospital, Chicago, IL, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Kalapurakal
authors.forename: A N
authors.initials: AN
authors.lastname: Jacob
authors.forename: P Y
authors.initials: PY
authors.lastname: Kim
authors.forename: D D
authors.initials: DD
authors.lastname: Najjar
authors.forename: Y C
authors.initials: YC
authors.lastname: Hsieh
authors.forename: P
authors.initials: P
authors.lastname: Ginsberg
authors.forename: I
authors.initials: I
authors.lastname: Daskal
authors.forename: S O
authors.initials: SO
authors.lastname: Asbell
authors.forename: R P
authors.initials: RP
authors.lastname: Kandpal
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D044383:Black People / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics*; D044465:White People / Q000235:genetics*; D001741:Black or African American
chemical_list: D005819:Genetic Markers; D017430:Prostate-Specific Antigen