Transposon-induced mutations in two loci of Listeria monocytogenes serotype 1/2a result in phage resistance and lack of N-acetylglucosamine in the teichoic acid of the cell wall.
Teichoic acid-associated N-acetylglucosamine and rhamnose have been shown to serve as phage receptors in Listeria monocytogenes serotype 1/2a. We generated and characterized two single-copy Tn916DeltaE mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified ptsHI locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was clpC, involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in N-acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected.
Tran
H L
HL
Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, USA.
Fiedler
F
F
Hodgson
D A
DA
Kathariou
S
S
D000117:Acetylglucosamine / Q000032:analysis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000145:classification / Q000472:pathogenicity*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D007113:Immunity, Innate; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics* / Q000821:virology*; D016254:Mutagenesis, Insertional; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000235:genetics; D016133:Polymerase Chain Reaction; D012703:Serotyping; D014161:Transduction, Genetic
D001426:Bacterial Proteins; C087481:ClpC protein, Bacteria; D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase; D000117:Acetylglucosamine