[The rapid diagnosis of toxigenic Vibrio cholerae strains in dot immunological analysis with monoclonal antibodies].
A method for identification of Vibrio cholerae toxigenic strains by dot immunoanalysis (DIA) with monoclonal antibodies to cholera toxin B-subunit is developed. It is based on analysis of lysates of isolated colonies of V. cholerae, grown in solid nutrient medium, broth cultures, and culture fluid. The analysis takes no more than 1-1.5 h, is highly sensitive, simple, and requires no special equipment and high qualification of specialists, which is of special importance in overall screening of humans and the environment for cholera.
Devdariani
Z L
ZL
Alenkina
T A
TA
Fedorova
V A
VA
D000818:Animals; D000911:Antibodies, Monoclonal; D002771:Cholera / Q000175:diagnosis*; D002772:Cholera Toxin / Q000032:analysis / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000276:immunology* / Q000472:pathogenicity
D000911:Antibodies, Monoclonal; D002772:Cholera Toxin