Denaturing gradient gel electrophoresis-based analysis of loss of heterozygosity distinguishes nonobvious, deleterious BRCA1 variants from nonpathogenic polymorphisms.
de La Hoya
M
M
Laboratory of Molecular Oncology, San Carlos University Hospital, 28040 Madrid, Spain.
Díaz-Rubio
E
E
Caldés
T
T
D019313:BRCA1 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006579:Heterozygote; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D019313:BRCA1 Protein; D004273:DNA, Neoplasm