Bioconjugation of laminin-related peptide YIGSR with polyvinyl pyrrolidone increases its antimetastatic effect due to a longer plasma half-life.
Polyvinyl pyrrolidone (PVP) which can be radically synthesized and have a long blood residency was used to modify the laminin-related peptide YIGSR, and its inhibitory effect on experimental lung metastasis of B16-BL6 melanoma cells was examined. The antimetastatic effect of PVP-conjugated YIGSR (PVP-YIGSR) was more than 100-fold greater than that of native YIGSR. When injected intravenously, PVP-YIGSR showed more than a 15-fold longer plasma half-life relative to native YIGSR. In addition, the stability of YIGSR in plasma was increased by conjugation with PVP. These findings suggest that PVP is a useful polymeric modifier for increasing the antimetastatic activity of YIGSR.
Mu
Y
Y
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Osaka, Suita, 565-0871, Japan.
Kamada
H
H
Kodaira
H
H
Sato
K
K
Tsutsumi
Y
Y
Maeda
M
M
Kawasaki
K
K
Nomizu
M
M
Yamada
Y
Y
Mayumi
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006207:Half-Life; D007797:Laminin; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000556:secondary*; D051379:Mice; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D010592:Pharmaceutic Aids / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D011205:Povidone / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004337:Drug Carriers; D007797:Laminin; D009842:Oligopeptides; D010592:Pharmaceutic Aids; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; D011205:Povidone