title: Two highly related homeodomain proteins, Nkx5-1 and Nkx5-2, display different DNA binding specificities.
abstract: The mouse Nkx5-1 and Nkx5-2 genes are related to NK genes in Drosophila and encode proteins with very similar homeodomains. In higher vertebrates Nkx5 genes are specifically expressed in the inner ear. Inactivation of the mouse Nkx5-1 gene by homologous recombination revealed a critical role for the formation of vestibular inner ear structures. Here, we investigated biochemical properties of the proteins encoded by the Nkx5 genes. A similar consensus binding sequence was isolated for both Nkx5 proteins using binding site selection. This sequence is related to target sequences previously identified for other Nkx proteins and contains the conserved homeodomain binding core TAAT. An additional, novel and unrelated high affinity binding sequence could be identified for the Nkx5-2 protein.
authors.affiliation: Technical University Braunschweig, Department of Cell and Molecular Biology, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Mennerich
authors.forename: S
authors.initials: S
authors.lastname: Hoffmann
authors.forename: T
authors.initials: T
authors.lastname: Hadrys
authors.forename: H H
authors.initials: HH
authors.lastname: Arnold
authors.forename: E
authors.initials: E
authors.lastname: Bober
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: C086188:Hmx2 protein, mouse; C086187:Hmx3 protein, mouse; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D005982:Glutathione Transferase