Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing.
The conserved CDC5 family of Myb-related proteins performs an essential function in cell cycle control at G(2)/M. Although c-Myb and many Myb-related proteins act as transcription factors, herein, we implicate CDC5 proteins in pre-mRNA splicing. Mammalian CDC5 colocalizes with pre-mRNA splicing factors in the nuclei of mammalian cells, associates with core components of the splicing machinery in nuclear extracts, and interacts with the spliceosome throughout the splicing reaction in vitro. Furthermore, genetic depletion of the homolog of CDC5 in Saccharomyces cerevisiae, CEF1, blocks the first step of pre-mRNA processing in vivo. These data provide evidence that eukaryotic cells require CDC5 proteins for pre-mRNA splicing.
Burns
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Department of Cell Biology, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA. geoff.burns@mcmail.vanderbilt.edu
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Krainer
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Gould
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D016475:3T3 Cells; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012322:RNA Precursors; D012326:RNA Splicing; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces; D029702:Schizosaccharomyces pombe Proteins; D017381:Spliceosomes; D013347:Subcellular Fractions; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D000097869:Polo-Like Kinase 1; C513239:CEF1 protein, S cerevisiae; C085875:cdc5 protein, S pombe