Ratio of remnant-like particle-cholesterol to serum total triglycerides is an effective alternative to ultracentrifugal and electrophoretic methods in the diagnosis of familial type III hyperlipoproteinemia.
BACKGROUND
Familial type III hyperlipoproteinemia (HLP) is characterized by the presence of beta-migrating VLDL (beta-VLDL) and increased risk of cardiovascular disease. Assessment of plasma beta-VLDL is achieved by measuring the ratio of VLDL-cholesterol (VLDL-C) to total plasma triglycerides (TGs) or by detecting beta-VLDL in total VLDL. The objective of this study was to compare the clinical utility of the ratio of remnant-like particle-cholesterol (RLP-C) to total TGs with that of the current methods for diagnosing type III HLP.


METHODS
Detection of beta-VLDL by electrophoresis of VLDL was used to define type III HLP. Twenty-eight patients with type III HLP and 43 subjects lacking beta-VLDL were investigated. Fasting TG concentrations were >2.26 mmol/L in all subjects. Subjects were separated into three groups: group 1, serum total cholesterol </=5.18 mmol/L (n = 11); group 2, total cholesterol >5.18 mmol/L and TGs between 2.26 and 9.04 mmol/L (n = 51); and group 3, TGs >9.04 mmol/L (n = 9).


RESULTS
In group 2, a RLP-C-to-total TG molar ratio >/=0.23 (>/=0.10 when using mg/dL) and a VLDL-C-to-total TG molar ratio >/=0.69 (>/=0.30 when using mg/dL) correctly classified 94% and 90% of the subjects, respectively. The utility of the RLP-C-to-total TG ratio in diagnosing type III HLP decreased in patients in the other two groups.


CONCLUSIONS
When used in an appropriate target population, the RLP-C-to-total TG ratio is a convenient and effective alternative to ultracentrifugal and electrophoretic methods for diagnosing type III HLP.
Wang
T
T
Otsuka America Pharmaceutical, Inc., 2440 Research Blvd., Rockville, MD 20850, USA. TAOW@MOCR.OAPI.com
Nakajima
K
K
Leary
E T
ET
Warnick
G R
GR
Cohn
J S
JS
Hopkins
P N
PN
Wu
L L
LL
Cilla
D D
DD
Zhong
J
J
Havel
R J
RJ
D001053:Apolipoproteins / Q000097:blood*; D002784:Cholesterol; D015243:Cholesterol, VLDL / Q000097:blood; D004586:Electrophoresis; D005260:Female; D006801:Humans; D006952:Hyperlipoproteinemia Type III / Q000097:blood / Q000175:diagnosis*; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D014280:Triglycerides / Q000097:blood*; D014461:Ultracentrifugation
D001053:Apolipoproteins; D015243:Cholesterol, VLDL; D008074:Lipoproteins; D014280:Triglycerides; C112017:remnant-like particle cholesterol; D002784:Cholesterol