title: Drosophila Lissencephaly-1 functions with Bic-D and dynein in oocyte determination and nuclear positioning.
abstract: Here we show that the Drosophila homologue of Lissencephaly-1, DLis-1, acts together with Bicaudal-D (Bic-D), Egalitarian (Egl), dynein and microtubules to determine oocyte identity. DLis-1 is further required for nurse-cell-to-oocyte transport during oocyte growth, and for the positioning of the nucleus in the oocyte. Immunostaining of DLis-1 protein reveals a cortical localization that is independent of microtubules. DLis-1 may function in this position as a cortical anchor for the other nuclear-localization factors. DLis-1 and Bic-D are further required for nuclear localization in the developing nervous system, indicating that homologues of Bic-D, dynein and Egl-like proteins may also be involved in vertebrate neural migration and that their absence may cause a Miller-Dieker-like lissencephaly.
authors.affiliation: Department of Biology, McGill University, 1205 Dr Penfield, Montreal, Quebec H3A 1B1, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Swan
authors.forename: T
authors.initials: T
authors.lastname: Nguyen
authors.forename: B
authors.initials: B
authors.lastname: Suter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D019476:Insect Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D017956:Photoreceptor Cells, Invertebrate / Q000502:physiology; D016415:Sequence Alignment
chemical_list: C061998:BicD protein, Drosophila; D029721:Drosophila Proteins; D019476:Insect Proteins; C513192:Lis-1 protein, Drosophila; D008869:Microtubule-Associated Proteins; C104613:egl protein, Drosophila; D004398:Dyneins