title: Estrogen stimulates neuronal nitric oxide synthase protein expression in human neutrophils.
abstract: Recent studies have postulated the contribution of nitric oxide (NO) released by the endothelium to the beneficial effects of estrogen. Despite a neuronal-type NO synthase (nNOS) described in neutrophils, less is known about the effect of estrogen in these cells. The aim of the present study was to analyze the expression of nNOS protein in human neutrophils under different estrogenic conditions. We first analyzed nNOS expression in neutrophils obtained from premenopausal women. During the first 2 days of the follicular phase (low circulating estrogen concentrations), nNOS expression in neutrophils was reduced with respect to that found in neutrophils obtained from the same donors during the ovulatory phase (high circulating estrogen concentrations). Moreover, the expression of nNOS protein in neutrophils obtained from postmenopausal women after transdermal estrogen therapy was markedly enhanced with respect to that observed before the treatment. In vitro incubation of neutrophils derived from men for 6 hours with 17beta-estradiol (10(-10) to 10(-8) mol/L) upregulated the expression of nNOS protein. The 17beta-estradiol receptor antagonists, tamoxifen (10(-8) mol/L) and ICI 182780 (10(-8) mol/L), inhibited the upregulation of nNOS protein induced by 17beta-estradiol. The putative functional implication was denoted by a reduced expression of the CD18 antigen on the surface of 17beta-estradiol-incubated neutrophils, which was accompanied by a decreased adhesive capacity. Both effects were prevented by an NO antagonist. In conclusion, the in vivo levels of circulating estrogen concentrations seem to be associated with the level of nNOS protein expression in neutrophils from women. Moreover, low doses of 17beta-estradiol upregulate nNOS protein expression in neutrophils from men. The increased ability of 17beta-estradiol-incubated neutrophils derived from men to produce NO reduced their adhesive properties.
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Durán
authors.forename: T
authors.initials: T
authors.lastname: de Frutos
authors.forename: J
authors.initials: J
authors.lastname: Díaz-Recasens
authors.forename: G
authors.initials: G
authors.lastname: García-Gálvez
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
authors.forename: M
authors.initials: M
authors.lastname: Montón
authors.forename: J
authors.initials: J
authors.lastname: Farré
authors.forename: L
authors.initials: L
authors.lastname: Sánchez de Miguel
authors.forename: F
authors.initials: F
authors.lastname: González-Fernández
authors.forename: M D
authors.initials: MD
authors.lastname: Arriero
authors.forename: L
authors.initials: L
authors.lastname: Rico
authors.forename: R
authors.initials: R
authors.lastname: García
authors.forename: S
authors.initials: S
authors.lastname: Casado
authors.forename: A
authors.initials: A
authors.lastname: López-Farré
mesh_terms: D000328:Adult; D000368:Aged; D018821:CD18 Antigens / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000378:metabolism / Q000517:prevention & control; D002448:Cell Adhesion / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D000077267:Fulvestrant; D020249:Hormone Replacement Therapy; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology*; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052248:Nitric Oxide Synthase Type I; D017698:Postmenopause; D017697:Premenopause; D011960:Receptors, Estrogen / Q000187:drug effects; D012727:Sex Characteristics; D013629:Tamoxifen / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D018821:CD18 Antigens; D004965:Estrogen Antagonists; D011960:Receptors, Estrogen; D013629:Tamoxifen; D000077267:Fulvestrant; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D004958:Estradiol; D007986:Luteinizing Hormone; C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I