title: The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105).
abstract: Mesenchymal stem cells are multipotent cells resident in the bone marrow throughout adulthood which have the capacity to differentiate into cartilage, bone, fat, muscle, and tendon. A number of monoclonal antibodies raised against human MSCs have been shown to react with surface antigens on these cells in vitro. A protein of molecular mass 92 kDa was immunoprecipitated using the SH-2 monoclonal antibody. This was purified and identified by peptide sequencing analysis and mass spectrometry as endoglin (CD105), the TGF-beta receptor III present on endothelial cells, syncytiotrophoblasts, macrophages, and connective tissue stromal cells. Endoglin on MSCs potentially plays a role in TGF-beta signalling in the control of chondrogenic differentiation of MSCs and also in mediating interactions between MSCs and haematopoietic cells in the bone marrow microenvironment.
authors.affiliation: Osiris Therapeutics Inc., 2001 Aliceanna Street, Baltimore, Maryland, 21231, USA. fbarry@osiristx.com
authors.forename: F P
authors.initials: FP
authors.lastname: Barry
authors.forename: R E
authors.initials: RE
authors.lastname: Boynton
authors.forename: S
authors.initials: S
authors.lastname: Haynesworth
authors.forename: J M
authors.initials: JM
authors.lastname: Murphy
authors.forename: J
authors.initials: J
authors.lastname: Zaia
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D000071063:Endoglin; D005333:Fetus; D006801:Humans; D046911:Macromolecular Substances; D008648:Mesoderm / Q000166:cytology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D011509:Proteoglycans / Q000032:analysis* / Q000737:chemistry; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013234:Stem Cells / Q000166:cytology*; D014357:Trypsin; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis* / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011509:Proteoglycans; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D019010:Vascular Cell Adhesion Molecule-1; C071736:betaglycan; D014357:Trypsin