Lack of dependence on p53 for DNA double strand break repair of episomal vectors in human lymphoblasts.
The p53 tumor suppressor gene has been shown to be involved in a variety of repair processes, and recent findings have suggested that p53 may be involved in DNA double strand break repair in irradiated cells. The role of p53 in DNA double strand break repair, however, has not been fully investigated. In this study, we have constructed a novel Epstein-Barr virus (EBV)-based shuttle vector, designated as pZEBNA, to explore the influence of p53 on DNA strand break repair in human lymphoblasts, since EBV-based vectors do not inactivate the p53 pathway. We have compared plasmid survival of irradiated, restriction enzyme linearized, and calf intestinal alkaline phosphatase (CIP)-treated pZEBNA with a Simian virus 40 (SV40)-based shuttle vector, pZ189, in TK6 (wild-type p53) and WTK1 (mutant p53) lymphoblasts and determined that p53 does not modulate DNA double strand break repair in these cell lines.
Kohli
M
M
Division of Radiation Research, Vincent T. Lombardi Cancer Center, Georgetown University Medical Center, 3970 Reservoir Road, NW, Washington, DC, 20007-2197, USA.
Jorgensen
T J
TJ
D002460:Cell Line; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D018014:Gene Transfer Techniques; D016147:Genes, Tumor Suppressor; D016158:Genes, p53; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes