Identification of a common site of provirus integration in radiation leukemia virus-induced T-cell lymphomas in mice.
The BL/VL(3) Kaplan radiation leukemia virus (RadLV-VL(3)) is a nondefective retrovirus that induces T cell lymphomas in several strains of mice. By using DNA probes derived from RadLV/VL(3) provirus-flanking sequences cloned from the BL/VL(3) cell line, we identified a DNA region rearranged in 5 of 19 tumors analysed (25%). All proviruses were integrated in the same 5'-to-3' orientation in a small DNA region called Kis1 (Kaplan integration site 1). This region was localized on distal mouse chromosome 2 in a region not previously identified as important to lymphomagenesis. The cells rearranged at the Kis1 locus represent a clonal subpopulation of the clonal tumor masses examined, indicating a probable role of Kis1 in tumor progression.
Lambert
J
J
Département des Sciences Biologiques, Université du Québec à Montréal, Québec, H3C-3P8, Canada.
Bergeron
D
D
Kozak
C A
CA
Rassart
E
E
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009115:Muridae; D011533:Proviruses / Q000235:genetics*; D016721:Radiation Leukemia Virus / Q000235:genetics*; D015183:Restriction Mapping; D012192:Retroviridae Infections / Q000235:genetics / Q000821:virology; D013601:T-Lymphocytes / Q000821:virology; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000235:genetics / Q000821:virology; D016662:Virus Integration