CD72-deficient mice reveal nonredundant roles of CD72 in B cell development and activation.
CD72, a B cell surface protein of the C-type lectin superfamily, recruits the tyrosine phosphatase SHP-1 through its ITIM motif(s). Using CD72-deficient (CD72-/-) mice, we demonstrate that CD72 is a nonredundant regulator of B cell development. In the bone marrow of CD72-/- mice, there was a reduction in the number of mature recirculating B cells and an accumulation of pre-B cells. In the periphery of CD72-/- mice, there were fewer mature B-2 cells and more B-1 cells. In addition, CD72 is a negative regulator of B cell activation, as CD72-/- B cells were hyperproliferative in response to various stimuli and showed enhanced kinetics in their intracellular Ca2+ response following IgM cross-linking.
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Department of Medicine, Stanford University School of Medicine, California 94305, USA.
Baumgarth
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Parnes
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D000818:Animals; D000917:Antibody Formation; D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000502:physiology*; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D001773:Blood Cells; D001853:Bone Marrow / Q000473:pathology; D020013:Calcium Signaling; D002454:Cell Differentiation; D005260:Female; D007075:Immunoglobulin M / Q000276:immunology; D007152:Immunologic Capping; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C064228:CD72 protein, human; D007075:Immunoglobulin M