Effect of short-term ethanol exposure on the suprachiasmatic nucleus of hypothalamus: immunohystochemical study in mice.
Morphological changes of the suprachiasmatic nucleus (SCN) of the hypothalamus were investigated in mice exhibiting intoxication signs of stages 2 or 3 after a short application term of 6% ethanol. Alterations in glial cells and neurons were examined using anti-glial fibrillary acidic protein (GFAP) and anti-calbindin D28k monoclonal antibody, respectively. The results revealed that short-term ethanol exposure led to strong expression of GFAP-immunoreactivity (GFAP-IR) in the dorsomedial part of the SCN. Furthermore, GFAP-IR astrocytes showed an increase in number and hypertrophy with longer processes. However, calbindin D28k-IR neurons were apparently little changed in the SCN. It is concluded that neuroadaptive response of astrocytes could occur before the neurotoxic effects emerge on neurons on the SCN.
Satriotomo
I
I
Department of Anatomy, Faculty of Medicine, Kagawa Medical University, 1750-1 Miki-cho, Kita-gun, Ikenobe, Kagawa 761-0793, Japan. irawan@kms.ac.jp
Miki
T
T
Itoh
M
M
Xie
Q
Q
Ameno
K
K
Takeuchi
Y
Y
D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000473:pathology; D064092:Calbindin 1; D064026:Calbindins; D000431:Ethanol / Q000009:adverse effects*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000737:chemistry / Q000473:pathology; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D013493:Suprachiasmatic Nucleus / Q000187:drug effects* / Q000473:pathology*; D013997:Time Factors
C576499:Calb1 protein, mouse; D064092:Calbindin 1; D064026:Calbindins; D005904:Glial Fibrillary Acidic Protein; D064030:S100 Calcium Binding Protein G; D000431:Ethanol