The effect of alginate and hyaluronate on the viability and function of immunoisolated neonatal rat islets.
Recent observations suggest that the extracellular matrix plays a role as regards the viability and morphological integrity in long-term culture of Langerhans islets. For the present study we encapsulated neonatal rat islets without extracellular matrix (WEM), with alginate solution (AL) and with hyaluronate solution (HY) into cuprophane hollow fibers. Function was tested after week 1 and 5. The insulin release of WEM encapsulated islets decreased significantly during the culture period. In contrast to this, AL and HY embedded islets had stable secretion values throughout the whole cultivation. Histological examination confirmed that viability of HY and of AL embedded islets differed significantly from that of WEM encapsulated islets. Furthermore, HY seems to be a more advantageous environment to immunoisolated islets than AL. Both the insulin secretion values and the viability of HY embedded islets were higher than of AL embedded islets. We conclude that an extracellular matrix is important for immunoisolated islets, to maintain their function and morphological integrity and that HY is especially suitable for this application.
Velten
F
F
Federal Research Centre, Institute of Physiology and Biochemistry of Nutrition, Kiel, Germany. velten@bafm.de
Laue
C
C
Schrezenmeir
J
J
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D007328:Insulin; D012996:Solutions; D020723:Glucuronic Acid; D006820:Hyaluronic Acid