[The isolation and characteristics of mutant Streptomyces globisporus 1912 defective for landomycin E biosynthesis].
Mutants defective in the synthesis of antitumor angucycline antibiotic landomycin E were obtained from the asporogenic and initial sporulating strains of Streptomyces globisporus 1912 by means of nitrosoguanidine treatment and UV-irradiation of the mycelium fragments and protoplasts. The frequency of induction of LanE mutants in the protoplasts of strain 3-1 was 3 times higher (0.55%) as compared to mycelium fragments (0.17%). After mutagenic treatment of spores the yield of such mutants was lower (0.044-0.071%). It was shown, that izoflavon daidzein was an effective and necessary regulator of the landomycin E synthesis. The collection of 53 landomycin defective mutants was divided into three groups on the basis of their ability to produce antibiotic and regulator: 1) mutants LanE-Dai+ (45 strains), which did not synthesize landomycin E, but produced daidzein; 2) mutants LanE+Dai- (4 strains), which could restore landomycin E synthesis after adding exogenic daidzein to the medium and 3) mutants LanE- Dai- which did not synthesize landomycin E in the presence of daidzein.
Matseliukh
B P
BP
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Lavrinchuk
V Ia
VIa
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004996:Ethidium / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000502:physiology*; D010641:Phenotype; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013171:Spores, Bacterial / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013302:Streptomyces / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D009153:Mutagens; C113593:landomycin E; D008769:Methylnitronitrosoguanidine; D004996:Ethidium