A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors.
We have cloned a novel 100-kDa mammalian protein, which was recognized by an anti-peptide antibody against an epitope-containing nuclear localization signal of NF-kappaB p65 subunit. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells.
Nishikimi
A
A
Laboratory of Reproductive Physiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Mukai
J
J
Kioka
N
N
Yamada
M
M
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D005656:Fungal Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000276:immunology; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae; D012568:Schizosaccharomyces; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D051996:Transcription Factor RelA
D000906:Antibodies; D002352:Carrier Proteins; D018076:DNA, Complementary; D000939:Epitopes; D005656:Fungal Proteins; D016328:NF-kappa B; D009687:Nuclear Proteins; C109024:PRP1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D051996:Transcription Factor RelA