Effect of guanethidine-induced adrenergic blockade on the different proteolytic systems in rat skeletal muscle.
Overall proteolysis and the activity of skeletal muscle proteolytic systems were investigated in rats submitted to guanethidine-induced adrenergic blockade for 4 days. In soleus, overall proteolysis increased by 15-20% during the first 2 days of guanethidine treatment but decreased to levels below control values after 4 days. Extensor digitorum longus (EDL) did not show the initial increase in total proteolysis, which was already reduced after 2 days of guanethidine treatment. The initial rise in the rate of protein degradation in soleus was accompanied by an increased activity of the Ca(2+)-dependent proteolytic pathway. In both soleus and EDL, the reduction in overall proteolysis was paralleled by decreased activities of the Ca(2+)-dependent and ATP-dependent proteolytic processes. No change was observed in the activity of the lysosomal proteolytic system. Overall proteolysis in soleus and EDL from nontreated rats was partially inhibited by isoproterenol, in vitro. The data suggest an acute inhibitory control of skeletal muscle proteolysis by the adrenergic system, well evident in the oxidative muscle, with an important participation of the Ca(2+)-dependent pathway.
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Department of Physiology, School of Medicine, University of São Paulo, 14049-900 Ribeirão Preto, São Paulo, Brazil.
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Migliorini
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Kettelhut
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D000255:Adenosine Triphosphate / Q000378:metabolism; D000313:Adrenal Medulla / Q000737:chemistry; D018663:Adrenergic Agents / Q000494:pharmacology*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D004298:Dopamine / Q000032:analysis / Q000097:blood; D010450:Endopeptidases / Q000378:metabolism; D004837:Epinephrine / Q000032:analysis / Q000097:blood; D006145:Guanethidine / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007545:Isoproterenol / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology*; D009638:Norepinephrine / Q000032:analysis / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood
D018663:Adrenergic Agents; D000318:Adrenergic beta-Agonists; D007328:Insulin; D013739:Testosterone; D000255:Adenosine Triphosphate; D010450:Endopeptidases; D007545:Isoproterenol; D002118:Calcium; D004298:Dopamine; D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine; D006145:Guanethidine