[The DNA of recombinant plasmids acquires mutagenic activity in a Bacillus subtilis culture after the insertion of the human genome Alu repeat].
In our previous work the mutagenic activity of recombinant plasmids (pBR322 carrying the human Alu repeat alone or in combination with preproinsulin or apo AI gene) in competent Bacillus subtilis culture was demonstrated. In present work it was shown that among seven tested plasmids only three revealed mutagenic activity (pBR322 and two Alu repeat-containing constructions). It seems that mutagenic activity is not inherent to any recombinant molecule in applied test system but depends on its structure. For example, Alu repeat of human genome may attach mutagenic properties to plasmids which either had no such properties, or lost them after gene engineering manipulations.
Karpova
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Pidpala
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Lukash
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D020087:Alu Elements / Q000187:drug effects* / Q000235:genetics; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000494:pharmacology*; D004274:DNA, Recombinant / Q000235:genetics / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D016254:Mutagenesis, Insertional / Q000187:drug effects* / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013329:Structure-Activity Relationship
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins