title: Relationship of cellular transmigration and airway response after allergen challenge.
abstract: We examined the relationship between eosinophil migration into the bronchoalveolar space and change in FEV(1) after endobronchial allergen challenge (EBAC) in atopic asthmatic (AA) and atopic nonasthmatic (ANA) subjects. The purpose of this study was to obtain continuous, intrasubject controlled assessment of the relationship between cell migration in control and allergen-challenged segments in the same individuals over 96 h. In AA subjects, the eosinophil (Eos) count in the bronchoalveolar lavage fluid (BALF) increased from a baseline of 7,896 +/- 3,865 to 416,476 +/- 231,012 Eos/ml by 72 h (p = 0.001) in the challenged segment post-EBAC. For ANA subjects, the postsegmental challenge count was 29,874 +/- 474 Eos/ml (p = 0.03 versus baseline and p < 0.05 AA peak versus ANA peak). In both groups, there was a comparable decrease in peripheral blood eosinophil count beginning 5 h after challenge, which resolved at 24 h. In AA subjects, 416,476 +/- 231,012 Eos/ml was obtained from the allergen-challenged segment and 23,522 +/- 8,298 Eos/ml was obtained from the sham-challenged segment (p < 0.001) at 72 h. In contrast, there was no difference in the Eos count obtained from the BALF between the antigen- and sham-challenged segments of ANA subjects. We also found that increased airway neutrophils were present in equal numbers in allergen-challenged and sham-challenged segments in both AA and ANA subjects. We conclude that augmented eosinophil migration after EBAC is a characteristic of atopic asthma and is not present in atopic subjects who do not have asthma. We find that BAL eosinophilia in ANA patients as well as neutrophilia in both ANA and AA subjects are nonspecific consequences of bronchoscopy. Finally, we find no relationship between specific airway eosinophil migration into the BALF and FEV(1) < 72 h after challenge; however, at 96 h, there is a substantial decrease in FEV(1) that accompanies BALF eosinophilia.
authors.affiliation: Section of Pulmonary and Critical Care Medicine, Committees on Cellular Physiology, Division of the Biological Sciences, The University of Chicago, Chicago, Illinois, USA. mndukwu@medicine.bsd.uchicago.edu
authors.forename: I M
authors.initials: IM
authors.lastname: Ndukwu
authors.forename: E T
authors.initials: ET
authors.lastname: Naureckas
authors.forename: C
authors.initials: C
authors.lastname: Maxwell
authors.forename: M
authors.initials: M
authors.lastname: Waldman
authors.forename: A R
authors.initials: AR
authors.lastname: Leff
mesh_terms: D000485:Allergens; D001249:Asthma / Q000150:complications / Q000276:immunology / Q000473:pathology*; D001980:Bronchi / Q000473:pathology; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D001999:Bronchoscopy; D002452:Cell Count; D002465:Cell Movement; D004804:Eosinophils / Q000473:pathology*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000150:complications / Q000276:immunology / Q000473:pathology; D007958:Leukocyte Count; D008297:Male; D016210:Methacholine Chloride; D009504:Neutrophils / Q000473:pathology; D011650:Pulmonary Alveoli / Q000473:pathology; D012882:Skin Tests
chemical_list: D000485:Allergens; D016210:Methacholine Chloride