Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer.
We report in this study with a pre-embedding immunogold method, the clustering of the group III metabotropic glutamate receptor 4a (mGluR4a) along the presynaptic membrane of parallel fiber synaptic terminals in the cerebellar molecular layer. The mGluR4a clusters were homogeneously distributed and interspaced by about 60 nm. These results suggest a particular arrangement of mGluR4a which might help to a rapid and effective activation of this receptor by glutamate.
Mateos
J M
JM
Department of Neurosciences, Faculty of Medicine and Dentistry, Basque Country University, Bilbao, Spain.
Elezgarai
I
I
Benítez
R
R
Osorio
A
A
Bilbao
A
A
Azkue
J J
JJ
Kuhn
R
R
Knöpfel
T
T
Grandes
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002460:Cell Line; D002531:Cerebellum / Q000648:ultrastructure*; D006801:Humans; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009474:Neurons / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D017729:Presynaptic Terminals / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis* / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis; D013570:Synaptic Membranes / Q000648:ultrastructure; D014162:Transfection
C088229:2-amino-4-phosphonobutyrate receptor; D000906:Antibodies; D010446:Peptide Fragments; D018094:Receptors, Metabotropic Glutamate; D011994:Recombinant Proteins