Use of ribonuclease VI from Artemia for the determination of cytidine 2'-phosphate.
An enzymatic method has been developed for the quantitative measurement of cytidine 2'-phosphate. Concentrations in the micromolar range can be measured even in the presence of at least five times greater concentrations of a variety of related nucleotides. The method is also suitable for detection of the 2',3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37) and its discrimination from the 2',3'-cyclic nucleotide 2'-phosphodiesterase (EC 3.1.4.16).
Díaz
A R
AR
Instituto de Investigaciones Biomédicas Alberto Sols, C.S.I.C., Facultad de Medicina U.A.M., Madrid, Spain.
Heredia
C F
CF
D000818:Animals; D001156:Artemia / Q000201:enzymology*; D003568:Cytidine Monophosphate / Q000032:analysis* / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D012260:Ribonucleases; D003568:Cytidine Monophosphate