Reduced pH causes structural changes in the potent mitogenic toxin of Pasteurella multocida.
Pasteurella multocida toxin is a potent mitogen that is believed to act intracellularly. On transverse urea gradient gels at pH 8.0 the toxin displayed one major unfolding transition at 4 M urea. However, at pH 6.1 the unfolding transition took place at 3.5 M urea. Circular dichroism spectra also indicated that a structural change took place at acidic pH. In addition it was found that the toxin that had been denatured in 8 M urea refolded in solution with a high recovery of biological activity. These findings are discussed in terms of the likely domain structure of the P. multocida toxin.
Smyth
M G
MG
Institute of Food Research, Whiteknights Road, Reading, Berkshire, UK.
Sumner
I G
IG
Lax
A J
AJ
D016475:3T3 Cells; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry*; D002942:Circular Dichroism; D016521:Electrophoresis, Gel, Pulsed-Field; D051379:Mice; D011489:Protein Denaturation; D017510:Protein Folding; D014508:Urea
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin; D014508:Urea