Cytopathic effect inhibition assay for determining the in-vitro susceptibility of herpes simplex virus to antiviral agents.
We compare a rapid dilution method for the determination of antiviral susceptibility of herpes simplex virus (HSV) with the plaque reduction assay. A total of 84 HSV clinical isolates were studied by both methods to detect in-vitro resistance to acyclovir and foscarnet. The rapid method showed for the detection of HSV isolates resistant to acyclovir and foscarnet, a sensitivity of 96. 8% and 100% and specificity of 100% and 100%, respectively. This method provides an easy and accurate screening procedure for the susceptibility testing of HSV to antiviral agents.
Cotarelo
M
M
Servicio de Microbiología Clínica y Enfermedades Infecciosas-VIH, Hospital General Universitario 'Gregorio Marañón', Madrid, Spain.
Catalán
P
P
Sánchez-Carrillo
C
C
Menasalvas
A
A
Cercenado
E
E
Tenorio
A
A
Bouza
E
E
D000212:Acyclovir / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D017245:Foscarnet / Q000494:pharmacology*; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity; D010948:Viral Plaque Assay / Q000379:methods*
D000998:Antiviral Agents; D017245:Foscarnet; D000212:Acyclovir