title: Effect of pancreaticojejunostomy on fibrosis, pancreatic blood flow, and interstitial pH in chronic pancreatitis: a feline model.
abstract: OBJECTIVE
  To study the relation between fibrosis, pancreatic blood flow (PMBF), interstitial pH (pHi), and the effects of pancreaticojejunostomy (PJ) in chronic pancreatitis.
  
  
  BACKGROUND
  Chronic pancreatitis is associated with low PMBF and pHi, suggesting the existence of underlying ischemia.
  
  
  METHODS
  In cats, the main pancreatic duct was partially obstructed and the animals were studied 2, 4, 6, and 8 weeks later. PJ was performed after 2 and 4 weeks of ductal obstruction and studied 4 weeks later. PMBF and pH were measured before and after stimulation with secretin and cholecystokinin. pHi was measured with microelectrodes, PMBF by hydrogen gas clearance. Histologic analysis of the pancreas with Sirius red (collagen stain) and fast green FCF (noncollagen protein) stains allowed semiquantitative analysis of the ratio between collagen and total protein (C/TP).
  
  
  RESULTS
  With the evolution of chronic pancreatitis, there is a progressive increase in the collagen content and C/TP ratio, a reduction in basal PMBF and pHi, and loss of the normal response to stimulation. Early PJ restores collagen content, C/TP ratio, and basal and stimulated PMBF and pHi to normal. PJ performed in established CP returns the C/TP ratio to normal, improves basal PMBF, and restores the normal hyperemic response to secretion. Basal pHi is improved and the "acid tide" associated with secretin returns, but there is still no response to cholecystokinin.
  
  
  CONCLUSIONS
  Pancreaticojejunostomy restores the elevated collagen and C/TP ratio to normal and reverses the ischemia present in CP. The authors speculate that restoration of PMBF and its normal response to stimulation allows "regeneration" and restoration of secretory function.
authors.affiliation: Department of Surgery, Sepulveda VA Medical Center, Los Angeles, California, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Patel
authors.forename: P U
authors.initials: PU
authors.lastname: Reber
authors.forename: M T
authors.initials: MT
authors.lastname: Toyama
authors.forename: S W
authors.initials: SW
authors.lastname: Ashley
authors.forename: H A
authors.initials: HA
authors.lastname: Reber
mesh_terms: D000818:Animals; D002415:Cats; D002908:Chronic Disease; D018450:Disease Progression; D005260:Female; D005355:Fibrosis; D006863:Hydrogen-Ion Concentration; D008297:Male; D010179:Pancreas / Q000098:blood supply* / Q000473:pathology*; D010193:Pancreaticojejunostomy; D010195:Pancreatitis / Q000378:metabolism* / Q000503:physiopathology*; D012039:Regional Blood Flow; D013997:Time Factors