title: Tissue tropism of avian reoviruses is genetically determined.
abstract: Two genome segments, M2 and S1, were preferentially selected in reassortants isolated in Vero cells. Analysis with monoclonal antibodies (MAbs) against RAM-1 strain showed that the 39-kDa protein encoded by the genome segment S1 contained epitopes involved in neutralisation of virus infectivity for both Vero and chicken kidney (CK) cells. The 39-kDa protein appeared to have two major epitopes that are attachment sites for cell receptors, one interacting only with CK cell receptors and the other with both CK and Vero cell receptors but principally Vero cell receptors. These results suggest that the strain RAM-1 may have developed an epitope for Vero cell receptors owing to mutation in the S1 genome segment, but still retained the epitope responsible for infection of CK cells.
authors.affiliation: School of Veterinary Studies, Murdoch University, Australia. jayesh@burnet.edu.au
authors.forename: J
authors.initials: J
authors.lastname: Meanger
authors.forename: R
authors.initials: R
authors.lastname: Wickramasinghe
authors.forename: C E
authors.initials: CE
authors.lastname: Enriquez
authors.forename: G E
authors.initials: GE
authors.lastname: Wilcox
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D002645:Chickens; D002522:Chlorocebus aethiops; D016679:Genome, Viral; D007668:Kidney; D018165:Orthoreovirus / Q000235:genetics* / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000235:genetics; D011991:Receptors, Virus / Q000502:physiology*; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D012330:RNA, Double-Stranded; D011991:Receptors, Virus