title: Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein.
abstract: The cytosolic ATPase N-ethylmaleimide-sensitive fusion protein (NSF) disassembles complexes of membrane-bound proteins known as SNAREs, an activity essential for vesicular trafficking. The amino-terminal domain of NSF (NSF-N) is required for the interaction of NSF with the SNARE complex through the adaptor protein alpha-SNAP. The crystal structure of NSF-N reveals two subdomains linked by a single stretch of polypeptide. A polar interface between the two subdomains indicates that they can move with respect to one another during the catalytic cycle of NSF. Structure-based sequence alignments indicate that in addition to NSF orthologues, the p97 family of ATPases contain an amino-terminal domain of similar structure.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: May
authors.forename: K M
authors.initials: KM
authors.lastname: Misura
authors.forename: S W
authors.initials: SW
authors.lastname: Whiteheart
authors.forename: W I
authors.initials: WI
authors.lastname: Weis
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D018360:Crystallography, X-Ray / Q000379:methods; D005033:Ethylmaleimide / Q000494:pharmacology*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D052066:N-Ethylmaleimide-Sensitive Proteins; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D050600:SNARE Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins; D000251:Adenosine Triphosphatases; D052066:N-Ethylmaleimide-Sensitive Proteins; D005033:Ethylmaleimide