Localization of the histamine H(2) receptor, a target for antiulcer drugs, in gastric parietal cells.
OBJECTIVE
Histamine H(2) receptor antagonists are widely used for the treatment of peptic ulcer disorders. However, whether the H(2) receptor is present in parietal or immune cells in the lamina propria remains controversial. This study is designed to determine the H(2) receptor localization immunohistochemically using an antibody against the newly cloned mouse histamine H(2) receptor.


METHODS
We cloned the mouse histamine H(2) receptor gene and generated a specific antipeptide antibody against the C terminus. Immunohistochemical studies were performed with this antibody and with a monoclonal antibody against H(+)/K(+) adenosine triphosphatase (ATPase).


RESULTS
Histamine H(2) receptors were localized on the plasma membrane and on the cytoplasm just beneath the plasma membrane on the basolateral sides of gastric cells. Confocal microscopy of double-stained sections using the monoclonal antibody against H(+)/K(+) ATPase, a specific parietal cell marker, showed that histamine H(2) receptors colocalized with H(+)/K(+) ATPase. No specific histamine H(2) receptor immunoreactivities were observed in the submucosal regions.


CONCLUSIONS
The H(2) receptor is localized in the gastric parietal cell.
Fukushima
Y
Y
3rd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan. fukushima-tky@umin.ac.jp
Ohmachi
Y
Y
Asano
T
T
Nawano
M
M
Funaki
M
M
Anai
M
M
Ogihara
T
T
Inukai
K
K
Onishi
Y
Y
Sakoda
H
H
Saitoh
T
T
Matsuhashi
N
N
Yazaki
Y
Y
Sugano
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000378:metabolism; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D011970:Receptors, Histamine H2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D000897:Anti-Ulcer Agents; D000911:Antibodies, Monoclonal; D017931:DNA Primers; D006635:Histamine H2 Antagonists; D011970:Receptors, Histamine H2; D017506:H(+)-K(+)-Exchanging ATPase