Measurement of protein synthesis in human tissues by the flooding method.
The flooding procedure for measuring protein synthesis in individual tissues with amino acids labelled with stable isotopes is explained, and its advantages for studies in humans are described. The application of this method for investigating the effects of nutrition in healthy volunteers and sick patients is illustrated with examples of studies on skeletal muscle, liver (including albumin) and lymphocytes.
Garlick
P J
PJ
Department of Surgery, State University of New York, Stony Brook 11794-8191, USA. garlick@surg.som.sunysb.edu
McNurlan
M A
MA
D015658:HIV Infections / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D011865:Radioisotope Dilution Technique; D014018:Tissue Distribution
D011506:Proteins