title: Interaction between calcium-activated chloride channels and the cystic fibrosis transmembrane conductance regulator.
abstract: We investigated interactions between cystic fibrosis conductance regulator (CFTR) and endogenous Ca2+-activated Cl- channels (CaCC) in bovine pulmonary artery endothelium (CPAE). CPAE cells, which do not express CFTR, were transiently transfected with wild-type (WT) CFTR and the deletion mutant deltaF508 CFTR. Currents through CaCC were significantly reduced after expression of WT CFTR. This inhibition was increased by stimulation (isobutylmethylxanthine, forskolin) of CFTR in cells expressing WT CFTR. There were no such effects when deltaF508 mutant CFTR, which is retained in the endoplasmic reticulum, was expressed. It is concluded that CFTR and CaCC are functionally coupled probably through a direct channel-channel interaction.
authors.affiliation: Laboratorium voor Fysiologie, KU Leuven, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Wei
authors.forename: A
authors.initials: A
authors.lastname: Vankeerberghen
authors.forename: H
authors.initials: H
authors.lastname: Cuppens
authors.forename: J
authors.initials: J
authors.lastname: Eggermont
authors.forename: J J
authors.initials: JJ
authors.lastname: Cassiman
authors.forename: G
authors.initials: G
authors.lastname: Droogmans
authors.forename: B
authors.initials: B
authors.lastname: Nilius
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000502:physiology*; D004553:Electric Conductivity; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D009154:Mutation; D011651:Pulmonary Artery / Q000378:metabolism; D014162:Transfection
chemical_list: D018118:Chloride Channels; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine