Synthesis of chitinase in Streptomyces thermoviolaceus is regulated by a two-component sensor-regulator system.
The chiS and chiR genes located upstream of the chitinase locus (chi40) on the chromosome of Streptomyces thermoviolaceus OPC-520 were cloned and sequenced. The deduced amino acid sequences revealed that ChiS (390 amino acids, 40.9 kDa) and ChiR (213 amino acids, 22 kDa) show significant sequence similarities to histidine kinases and response regulators, respectively, of typical prokaryotic two-component regulatory systems. The extracellular chitinase activity of Streptomyces lividans 66 (pTSR2 (bearing chiS, chiR and chi40)) was significantly enhanced by a high dosage of the chiS and chiR genes.
Tsujibo
H
H
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, Japan. tsujibo@oysun01.oups.ac.jp
Hatano
N
N
Okamoto
T
T
Endo
H
H
Miyamoto
K
K
Inamori
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002688:Chitinases / Q000096:biosynthesis*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011494:Protein Kinases; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014157:Transcription Factors
D001426:Bacterial Proteins; C403318:ChiR protein, Streptomyces; D014157:Transcription Factors; D011494:Protein Kinases; C403319:ChiS protein, Streptomyces thermoviolaceus; D002688:Chitinases