title: Concanavalin A-binding cholesterol crystallization inhibiting and promoting activity in bile from patients with Crohn's disease compared to patients with ulcerative colitis.
abstract: OBJECTIVE
  Crohn's disease is a risk factor for gallstone formation. In contrast, patients with ulcerative colitis have an incidence of gallstone formation comparable to the general population. The reason for this difference is not known. The aim of this study was to elucidate the factors controlling cholesterol crystallization in gallbladder bile of Crohn's disease and ulcerative colitis patients.
  
  
  METHODS
  Gallbladder bile was obtained by aspiration during bowel resections (26 Crohn's disease patients, 20 ulcerative colitis patients). Biliary lipid composition, crystal detection time and the effect of extraction of the concanavalin A-binding fraction on crystal formation were determined.
  
  
  RESULTS
  Cholesterol crystals were present in seven of the 26 bile samples of Crohn's disease-patients and one of the 20 ulcerative colitis patients. Four of the bile samples of Crohn's disease patients were fast nucleating. None of the 20 ulcerative colitis patients had fast nucleating bile. Lipid composition, total lipid concentration and CSI were not significantly different between the two groups. In Crohn's disease patients extraction of concanavalin A-binding fraction decreased crystallization in 10 bile samples but accelerated crystallization in one bile sample. In eight bile samples from ulcerative colitis patients crystallization increased after concanavalin A-binding fraction extraction.
  
  
  CONCLUSIONS
  Compared to ulcerative colitis patients, gallbladder bile of Crohn's disease patients showed increased cholesterol crystallization despite comparable lipid composition and cholesterol saturation index. This difference is caused by increased cholesterol crystallization-promoting activity. Bile from ulcerative colitis patients contains a Con A-binding factor which inhibits cholesterol crystallization.
authors.affiliation: Department of Surgery, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Y C
authors.initials: YC
authors.lastname: Keulemans
authors.forename: K S
authors.initials: KS
authors.lastname: Mok
authors.forename: J F
authors.initials: JF
authors.lastname: Slors
authors.forename: M A
authors.initials: MA
authors.lastname: Brink
authors.forename: D J
authors.initials: DJ
authors.lastname: Gouma
authors.forename: G N
authors.initials: GN
authors.lastname: Tytgat
authors.forename: A K
authors.initials: AK
authors.lastname: Groen
mesh_terms: D000328:Adult; D001646:Bile / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000378:metabolism*; D003208:Concanavalin A / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism*; D003460:Crystallization; D005260:Female; D005704:Gallbladder / Q000378:metabolism; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D012635:Secretory Component / Q000378:metabolism
chemical_list: D007070:Immunoglobulin A; D012635:Secretory Component; D003208:Concanavalin A; D002784:Cholesterol