High-throughput plasmid DNA purification for 3 cents per sample.
To accommodate the increasingly rapid rates of DNA sequencing we have developed and implemented an inexpensive, expeditious method for the purification of double-stranded plasmid DNA clones. The robust nature, high throughput, low degree of technical difficulty and extremely low cost have made it the plasmid DNA preparation method of choice in both our expressed sequence tag (EST) and genome sequencing projects. Here we report the details of the method and describe its application in the generation of more than 700 000 ESTs at a rate exceeding 16 000 per week.
Marra
M A
MA
Washington University Genome Sequencing Center, 4444 Forest Park Boulevard, St Louis, MI 63108, USA. mmarra@alu.wustl.edu
Kucaba
T A
TA
Hillier
L W
LW
Waterston
R H
RH
D004269:DNA, Bacterial / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D005821:Genetic Techniques; D008872:Microwaves; D010957:Plasmids / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA / Q000379:methods
D004269:DNA, Bacterial; D004274:DNA, Recombinant