title: Analysis of exogenous dehydroepiandrosterone excretion in urine by gas chromatography/combustion/isotope ratio mass spectrometry.
abstract: A detailed procedure for the analysis of exogenous dehydroepiandrosterone (DHEA) in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) has been established for detecting doping with DHEA. The average delta-value (parts per thousand difference of (13)C/(12)C ratio from the isotope ratio standard) of 26 synthetic steroids commercially available was -30.1 +/- 2.6, and was significantly lower than that of human endogenous DHEA in urine of the world class athletes who had participated in the XVIIth Olympic Winter Games (-20.3 +/- 2.1, n = 446). Although large inter-individual variations of urinary DHEA excretion were observed following a single oral administration of 50 mg of DHEA, no significant inter-individual difference was found when the excretion of exogenous DHEA was monitored in terms of delta-values using GC/C/IRMS; the minimum delta-values were observed around 6-8 h after the administration, and the values returned to the base level at over 72 h after the dosing. Thus, the deviations in delta-values of DHEA and its diol metabolites are considered to be conclusive evidence for detecting doping with DHEA. Some successful cases of detection of doping with DHEA from athletes are also reported.
authors.affiliation: Doping Control Laboratory, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., 3-30-1 Shimura, Itabashi-ku, Tokyo 174-8555, Japan. wd3m-uek@asahi-net.or.jp
authors.forename: M
authors.initials: M
authors.lastname: Ueki
authors.forename: M
authors.initials: M
authors.lastname: Okano
mesh_terms: D000284:Administration, Oral; D003687:Dehydroepiandrosterone / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000652:urine*; D013196:Dihydrotestosterone / Q000652:urine; D004300:Doping in Sports; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006728:Hormones / Q000652:urine; D006801:Humans; D007564:Japan; D007700:Kinetics; D008297:Male; D012016:Reference Values; D013177:Sports; D015813:Substance Abuse Detection / Q000379:methods; D013739:Testosterone / Q000652:urine
chemical_list: D006728:Hormones; D013196:Dihydrotestosterone; D013739:Testosterone; D003687:Dehydroepiandrosterone