Selection of quantitative criteria for evaluating metastases of a highly metastatic salivary adenoid cystic carcinoma cell line to the lungs of nude mice.
OBJECTIVE
To select reliable quantitative criteria for evaluating metastases of a highly metastatic salivary adenoid cystic carcinoma cell line (ACC-M) cell to the lungs of nude mice.


METHODS
The within-group coefficients of correlation of 5 parameters were measured from lung slides using a video image digital analysis system (VIDAS), and the intergroup coefficients of correlation of 6 sets of parameters from all the lungs, which were taken from ACC-M lung metastases models, were calculated and statistically analyzed.


RESULTS
The within-group coefficients of correlation of 5 parameters are as follows: ratio of area of metastases, t = 0.9 (P < 0.001); area of metastases, t = 0.7 (P < 0.05), size of lung section, numbers of metastases, and size of the largest metastatic focus all t < 0.4 (P > 0.05). The intergroup coefficients of correlation of the parameters are as follows: ratio of area of metastases was assigned 1, weight of the lung was 0.9755, and area of metastases was 0.8628.


CONCLUSIONS
For evaluating lung metastases of ACC-M, the ratio of area of metastases and weight of the lung are precise; the area of metastases is also significant within the 6 quantitative parameters.
Gu
Z
Z
Department of Oral and Maxillofacial Surgery, Shanghai Ninth Hospital, Shanghai Second Medical University (SSMU), P. R. China.
Qiu
W
W
He
R
R
Zhang
Z
Z
Zhou
X
X
D000818:Animals; D001241:Aspirin / Q000627:therapeutic use; D003528:Carcinoma, Adenoid Cystic / Q000188:drug therapy / Q000473:pathology / Q000556:secondary*; D018450:Disease Progression; D005260:Female; D007091:Image Processing, Computer-Assisted; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014407:Tumor Cells, Cultured; D014741:Video Recording
D001241:Aspirin