Cloning of a Leishmania major gene encoding for an antigen with extensive homology to ribosomal protein S3a.
Following purification by affinity chromatography, a Leishmania major S-hexylglutathione- binding protein of molecular mass 66kDa was isolated. The immune serum against the parasite 66kDa polypeptide when used to screen a L. major cDNA library could identify clones encoding for the human v-fos transformation effector homologue, namely ribosomal protein S3a, and thus was named LmS3a-related protein (LmS3arp). A 1027bp cDNA fragment was found to contain the entire parasite gene encoding for a highly basic protein of 30kDa calculated molecular mass sharing homology to various ribosomal S3a proteins from different species. Using computer methods for a multiple alignment and sequence motif search, we found that LmS3arp shares a sequence homology to class theta glutathione S-transferase mainly in a segment containing critical residues involved in glutathione binding. These new findings are discussed in the light of recent published data showing multiple function(s) of the ribosomal proteins S3a.
Zemzoumi
K
K
CJF-INSERM n degrees 96-04, Centre IRD, 911 Av. Agropolis, BP 5045, 34032, Montpellier, France.
Guilvard
E
E
Sereno
D
D
Preto
A
A
Benlemlih
M
M
Da Silva
A C
AC
Lemesre
J L
JL
Ouaissi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015723:Gene Library; D017125:Genes, Protozoan / Q000235:genetics*; D005978:Glutathione / Q000378:metabolism; D018320:Leishmania major / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011485:Protein Binding; D015800:Protozoan Proteins; D012269:Ribosomal Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013462:Sulfur Radioisotopes
D000906:Antibodies; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D018076:DNA, Complementary; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C488930:Rsp3a1 protein, mouse; C402626:S3a-related protein, Leishmania major; D013462:Sulfur Radioisotopes; D005978:Glutathione