Effects of dextranation on the pharmacokinetics of short peptides. A PET study on mEGF.
The effects of dextranation on the biodistribution of mouse epidermal growth factor (mEGF, 6 kDa) were assessed. By reductive amination, mEGF was coupled to 13 and 46 kDa dextran. The two dextranated conjugates and free mEGF were labeled with the positron-emitting nuclide (76)Br (T(1/2) = 16 h). After intravenous administration to Sprague Dawley rats, the radioactivity biodistribution was evaluated by positron emission tomography (PET) and by measurements of dissected tissues. The dextranation prolonged the retention time in blood, especially when the dextran chain was long. [(76)Br]mEGF-dextran conjugates were shown to have significantly, more than 5 times, lower kidney accumulation than the nonconjugated [(76)Br]mEGF. In conclusion, dextranation affects the biodistribution of mEGF in vivo giving a prolonged circulation time, a decreased uptake in kidney, and an increased spleen accumulation.
Zhao
Q
Q
Biomedical Radiation Sciences, Box 535, Uppsala University, S-751 21 Uppsala, Sweden.
Tolmachev
V
V
Carlsson
J
J
Lundqvist
H
H
Sundin
J
J
Janson
J C
JC
Sundin
A
A
D000818:Animals; D001967:Bromine Radioisotopes; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed
D001967:Bromine Radioisotopes; D003911:Dextrans; D004815:Epidermal Growth Factor