[Flow cytometry and pathology AFC-089. Fluorescence recovery after photobleaching: principle and applications. An example: research of gap junctions between immature sensory cells of the cochlea].
In image cytometry, fluorescence recovery after photobleaching (FRAP) is wildly used to study molecular displacements at tissular, cellular or infracellular levels. The principle and the main applications of FRAP are presented. An example is given: the investigation of functional communications between cochlea's immature sensory cells.
Vago
P
P
Université Montpellier I, Faculté de Médecine de Montpellier-Nîmes, France. vago@infobiogen.fr
Ripoll
C
C
Lenoir
M
M
D003051:Cochlea / Q000294:innervation*; D005434:Flow Cytometry; D005453:Fluorescence; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D009475:Neurons, Afferent / Q000502:physiology*; D010777:Photochemistry