Open reading frame 1 of the Norwalk-like virus Camberwell: completion of sequence and expression in mammalian cells.
The ORF1 sequence was determined for Camberwell virus, a genogroup 2 Norwalk-like virus, completing the full genome of 7,555 nucleotides. ORF1 cDNA was cloned into a simian virus 40-based expression vector, and the viral proteins synthesized following transfection into COS cells were analyzed. By using antisera directed against the helicase, protease, or polymerase regions, eight polypeptides ranging in size from 19 to 117 kDa were detected by radioimmunoprecipitation. The cleavage sites determining the amino and carboxy termini of the 3C-like protease were identified at E(1008)/A and E(1189)/G, respectively.
Seah
E L
EL
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
Marshall
J A
JA
Wright
P J
PJ
D000086042:3C Viral Proteases; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003546:Cysteine Endopeptidases / Q000235:genetics; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000235:genetics*; D016366:Open Reading Frames; D017354:Point Mutation; D020365:RNA Helicases / Q000235:genetics; D014764:Viral Proteins
D004279:DNA, Viral; D014764:Viral Proteins; C116598:2C helicase; D012321:DNA-Directed RNA Polymerases; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases; D020365:RNA Helicases