New class of polymers for the delivery of macromolecular therapeutics.
Cationic polymers show promise for the in vitro and in vivo delivery of macromolecular therapeutics. Known cationic polymers, e.g., poly(L)lysine (PLL) and polyethylenimine (PEI), have been employed in native and modified forms for the delivery of plasmid DNA (pDNA) and reveal varying levels of toxicity. Here, we report the preparation of a new class of cationic polymers that are specifically designed to deliver macromolecular therapeutics. Linear, cationic, beta-cyclodextrin (beta-CD)-containing polymers (CD-polymers) are synthesized by copolymerizing difunctionalized beta-CD monomers (AA) with other difunctionalized comonomers (BB) such that an AABBAABB product is formed. The beta-CD polymers are able to bind approximately 5 kbp pDNA above polymer to DNA (+/-) charge ratios of 1.5, compact the bound pDNA into particles of approximately 100-150 nm in size at charge ratios above 5+/-, and transfect cultured cells at charge ratios above 10+/-. In vitro transfections with the new beta-CD-polymers are comparable to the best results obtained in our hands with PEI and Lipofectamine. Some cell line-dependent toxicities are observed for serum-free transfections; however, no toxicity is revealed at charge ratios as high as 70+/- in transfections conducted in 10% serum. Single IV and IP doses as high as 200 mg/kg in mice showed no mortalities.
Gonzalez
H
H
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Hwang
S J
SJ
Davis
M E
ME
D000818:Animals; D016466:CHO Cells; D002412:Cations; D016923:Cell Death; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006224:Cricetinae; D003505:Cyclodextrins / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers; D007668:Kidney; D008027:Light; D046911:Macromolecular Substances; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D014162:Transfection; D047392:beta-Cyclodextrins
D002412:Cations; D003505:Cyclodextrins; D004337:Drug Carriers; D046911:Macromolecular Substances; D011108:Polymers; D047392:beta-Cyclodextrins; D004247:DNA; C031215:betadex