Expression of 5-HT2A and 5-HT1B receptor mRNA in blood vessels.
Expression of two subtypes of 5-hydroxytryptamine (5-HT) receptors, 5-HT1B and 5-HT2A, was investigated in normal rat arteries (abdominal aorta) and veins (inferior vena cava) by the quantitative reverse transcription-polymerase chain reaction (RT-PCR). The arterial 5-HT2A receptor mRNA level in artery was 25-fold (p < 0.01) higher than the venous level, and the arterial 5-HT1B receptor mRNA level was at least 7000-fold (p < 0.005) higher the venous level.
Kato
S
S
The First Department of Internal Medicine, Dokkyo University School of Medicine, Tochigi Prefecture Japan.
Kumamoto
H
H
Hirano
M
M
Akiyama
H
H
Kaneko
N
N
D000818:Animals; D001158:Arteries / Q000502:physiology; D001808:Blood Vessels / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008297:Male; D008970:Molecular Weight; D010446:Peptide Fragments / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044302:Receptor, Serotonin, 5-HT1B; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods; D014680:Veins / Q000502:physiology
D005456:Fluorescent Dyes; D010446:Peptide Fragments; D012333:RNA, Messenger; D044302:Receptor, Serotonin, 5-HT1B; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases