title: Oxygen radical production by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils.
abstract: Oxidative metabolic burst activity by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils was indirectly evaluated by measuring the oxidation of nonfluorescent intracellular 2',7'-dichlorofluorescein (DCFH) to fluorescent 2',7'-dichlorofluorescein (DCF) with flow cytometry. The results were recorded as mean channel fluorescence intensity. Phorbol myristate acetate at 50 ng/ml was used to activate the cells. Elephant heterophils and bovine neutrophils exhibited similar abilities to oxidize intracellular DCFH to DCF, a fluorescent product. A wide range of mean channel fluorescence intensity (1,803-7,393) was observed among individual elephants, and the range of intensities was similar to that observed in bovine neutrophils, supporting the concept of functional heterogeneity of heterophils among elephants.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.
authors.forename: L
authors.initials: L
authors.lastname: Tell
authors.forename: M B
authors.initials: MB
authors.lastname: Kabbur
authors.forename: W L
authors.initials: WL
authors.lastname: Smith
authors.forename: L
authors.initials: L
authors.lastname: Gage
authors.forename: J S
authors.initials: JS
authors.lastname: Cullor
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood* / Q000378:metabolism; D004606:Elephants / Q000097:blood* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000502:physiology*
chemical_list: D005452:Fluoresceins; D005609:Free Radicals; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; C037631:2',7'-dichlorofluorescein