title: Inhibitory effect of tea flavonoids on the ability of cells to oxidize low density lipoprotein.
abstract: Dietary flavonoid intake has been reported to be inversely related to mortality from coronary heart disease, and the anti-atherosclerotic effect of flavonoids is considered to be due probably to their antioxidant properties. Oxidation of low density lipoprotein (LDL) has been reported to be induced by the constituent cells of the arterial wall. Accordingly, we examined the effect of pretreatment with tea flavonoids, such as theaflavin digallate, on the ability of cells to oxidize LDL. Theaflavin digallate pretreatment of macrophages or endothelial cells reduced cell-mediated LDL oxidation in a concentration- (0-400 microM) and time- (0-4 hr) dependent manner. This inhibitory effect of flavonoids on cell-mediated LDL oxidation was in the order of theaflavin digallate > theaflavin > or = epigallocatechin gallate > epigallocatechin > gallic acid. Further, we investigated the mechanisms by which flavonoids inhibited cell-mediated LDL oxidation using macrophages and theaflavin digallate. Theaflavin digallate pretreatment decreased superoxide production of macrophages and chelated iron ions significantly. These results suggest that tea flavonoids attenuate the ability of the cell to oxidize LDL, probably by reducing superoxide production in cells and chelating iron ions.
authors.affiliation: First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Hosoai
authors.forename: M
authors.initials: M
authors.lastname: Suzukawa
authors.forename: M
authors.initials: M
authors.lastname: Ayaori
authors.forename: T
authors.initials: T
authors.lastname: Hisada
authors.forename: S
authors.initials: S
authors.lastname: Sawada
authors.forename: A
authors.initials: A
authors.lastname: Yonemura
authors.forename: K
authors.initials: K
authors.lastname: Higashi
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Tomiyasu
authors.forename: M
authors.initials: M
authors.lastname: Nishiwaki
authors.forename: F
authors.initials: F
authors.lastname: Ohsuzu
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D044946:Biflavonoids; D002392:Catechin; D002470:Cell Survival / Q000187:drug effects; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D005419:Flavonoids; D008077:Lipoproteins, LDL; D013662:Tea; D013481:Superoxides; C077197:theaflavin digallate; D005707:Gallic Acid; D002392:Catechin; D007501:Iron