Allelic imbalance within the E-cadherin gene is an infrequent event in prostate carcinogenesis.
By exploiting two single nucleotide polymorphisms (SNPs) located within the E-cadherin gene, at 16q22, we have determined the frequency of allelic imbalance at this proposed tumor suppressor locus in a series of human prostatic carcinoma DNA samples. Whereas results with seven highly polymorphic microsatellite markers flanking the E-cadherin locus confirmed the existence of three separate loci on chromosome 16, at which allelic imbalance increased with increasing loss of tumor cell differentiation, no allelic imbalance within the E-cadherin gene was detected either by single-strand conformational polymorphism analysis or by direct sequencing. We conclude that the loss of E-cadherin function observed in prostate cancer is not a result of allelic deletion. Genes Chromosomes Cancer 27:104-109, 2000.
Murant
S J
SJ
YCR Cancer Research Unit, Department of Biology, University of York, York, United Kingdom.
Rolley
N
N
Phillips
S M
SM
Stower
M
M
Maitland
N J
NJ
D000483:Alleles; D015820:Cadherins / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017384:Sequence Deletion
D015820:Cadherins