Analysis of vervain flavonoids by HPLC/Diode array detector method. Its application to quality control.
A reversed-phase HPLC procedure is proposed for the determination of seven flavonoids (luteolin, nepetin, hispidulin, jaceosidin, cirsimaritin, cirsilineol, and eupatorin) in vervain samples. A simple extractive technique was developed, involving only extraction with ethyl ether, which allowed the elimination of phenolic acids, yielding a sufficiently purified flavonoid fraction. All of the analyzed vervain samples showed a common flavonoid pattern, in which hispidulin and jaceosidin were the major compounds and nepetin was the minor compound.
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CEQUP/Laboratorio de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 4050 Porto, Portugal.
Andrade
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Seabra
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047309:Flavones; D005419:Flavonoids / Q000032:analysis*; D047311:Luteolin; D010936:Plant Extracts / Q000737:chemistry*
D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts; C103110:eupatorin; C057618:cirsilineol; C007072:cirsimaritin; D047311:Luteolin; C055957:hispidulin