Enzymatic degradation of neuropeptide FF and SQA-neuropeptide FF in the mouse brain.
Degradation of neuropeptide FF (NPFF) and SQA-neuropeptide FF (SQA-NPFF) by mouse brain sections was investigated by using capillary electrophoresis with UV detection for the separation and the identification of the degradation products. The half disappearance time of SQA-NPFF was 2-fold greater than that of NPFF. NPFF was cleaved preferentially into an inactive metabolite, Gln-Arg-Phe-NH2, in the cerebrum slices. SQA-NPFF was hydrolyzed by an unidentified degrading activity to generate NPFF, and NPFF accounted for a larger part of SQA-NPFF degradation in the hindbrain and cervical spinal cord than in the cerebrum slices. These findings suggest that, depending on the brain regions, NPFF produced from SQA-NPFF could prolong the biologic effects of SQA-NPFF.
Sol
J C
JC
Institut de Pharmacologie et de Biologie Structurale, CNRS, Toulouse, France.
Roussin
A
A
Proto
S
S
Mazarguil
H
H
Zajac
J M
JM
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002268:Carboxypeptidases / Q000378:metabolism; D019075:Electrophoresis, Capillary; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000378:metabolism*
D009842:Oligopeptides; C115685:seryl-glutaminyl-alanyl-phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide; D002268:Carboxypeptidases; D007930:Leucine; C012211:ubenimex