Determination of the antibiotic fungicide validamycin A in formulated products by micellar electrokinetic chromatography.
A micellar electrokinetic capillary chromatographic method (MEKC) was used to determine validamycin A content in commercial products. The results indicated that this method was capable of analyzing the validamycin A content in formulated products with an instrument detection limit of 0.94 microg/mL and a method detection limit of 1. 70 microg/mL. Relative standard deviation (RSD) values of MEKC determination of validamycin A in formulated products ranged from 0. 61 to 2.09%. Recoveries of validamycin A in formulated products were in the region of 99.5-105.1%. All commercial products collected from markets contained validamycin A. The high percentage of recovery, the low detection limit, and the low RSD values confirmed that the MEKC technique is a senstivie and selective method.
Hsiao
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Pesticide Chemistry Department, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, 11 Kuang Ming Road, Wufeng, Taichung Hsien, Taiwan, Republic of China.
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D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002626:Chemistry, Pharmaceutical; D002845:Chromatography / Q000379:methods; D004563:Electrochemistry; D007294:Inositol / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D008823:Micelles; D008969:Molecular Sequence Data
D000900:Anti-Bacterial Agents; D008823:Micelles; C030790:validamycin A; D007294:Inositol