The effects of an alpha hydroxy acid (glycolic acid) on hairless guinea pig skin permeability.
The barrier integrity of hairless guinea pig skin after treatment with an alpha hydroxy acid was assessed through in vivo topical application of an oil-in-water emulsion containing 5 or 10% glycolic acid at pH 3.0. The control was a commercial moisturizing lotion, pH 7.8. A dosing regimen for the glycolic acid formulations that was tolerated by the hairless guinea pigs and significantly decreased stratum corneum turnover time was determined using the dansyl chloride staining technique. Once-daily dosing of hairless guinea pig skin for 3 weeks with the glycolic acid formulations resulted in approximately a 36-39% decrease in stratum corneum turnover time compared with the control lotion. After this treatment, hairless guinea pigs were sacrificed for the in vitro measurement of the percutaneous absorption of [14C]hydroquinone and [14C]musk xylol. No significant differences in the 24-hour absorption of either test compound were found for skin treated with the control lotion or the glycolic acid formulations. There were also no significant differences found in the absorption of [3H]water through skin from the different treatment groups. Although no increase in skin penetration occurred after treatment with the glycolic acid formulations, histology revealed approximately a twofold increase in epidermal thickness. Also the number of nucleated cell layers nearly doubled in skin treated with 5% and 10% glycolic acid compared with the control lotion and untreated skin. These studies demonstrate that substantial changes in the structure of hairless guinea pig epidermis can occur without significant effect on skin permeability of two model compounds.
Hood
H L
HL
Office of Cosmetics & Colors, Food and Drug Administration, Laurel, MD 20708, USA.
Kraeling
M E
ME
Robl
M G
MG
Bronaugh
R L
RL
D000287:Administration, Topical; D000818:Animals; D003619:Dansyl Compounds / Q000493:pharmacokinetics; D006016:Glycolates / Q000633:toxicity*; D006168:Guinea Pigs; D006873:Hydroquinones / Q000493:pharmacokinetics; D007641:Keratolytic Agents / Q000633:toxicity*; D009824:Ointments; D010539:Permeability; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D012869:Skin Absorption / Q000187:drug effects*; D014992:Xylenes / Q000493:pharmacokinetics
D003619:Dansyl Compounds; D006016:Glycolates; D006873:Hydroquinones; D007641:Keratolytic Agents; D009824:Ointments; D014992:Xylenes; C031149:glycolic acid; C031447:musk xylene; C029038:dansyl chloride; C031927:hydroquinone