title: Bovine blood components: fractionation, composition, and nutritive value.
abstract: The objective of this research work was to fractionate bovine blood, collected hygienically in a slaughterhouse, into blood plasma protein concentrate, red blood cell concentrate, globin isolate, and a carboxymethylcellulose-heme iron (CMC-heme) complex. All four fractions were studied for proximate composition and amino acid and mineral contents. The nutritive value of plasma protein concentrate and globin isolate was comparatively studied using rat bioassays. The amino acid content in plasma protein concentrate is well balanced and produced net protein utilization and net protein ratio equivalent to 95% those of casein. Globin isolate ( approximately 91% protein) is deficient in isoleucine and S-containing amino acids and was unable to support rat growth at 10% concentration in the diet. Red blood cell concentrate and the isolated CMC-heme complex were good sources of bioavailable iron. Iron availabilities for CMC-heme and whole blood cell concentrate, related to ferrous sulfate as 100%, were 64 and 70%, respectively.
authors.affiliation: Food Chemistry and Applied Nutrition Center, Institute of Food Technology (ITAL), Av. Brasil 2880, CEP 13073-001, Campinas, São Paulo, Brazil.
authors.forename: R T
authors.initials: RT
authors.lastname: Duarte
authors.forename: M C
authors.initials: MC
authors.lastname: Carvalho Simões
authors.forename: V C
authors.initials: VC
authors.lastname: Sgarbieri
mesh_terms: D000740:Anemia / Q000178:diet therapy; D000818:Animals; D001769:Blood; D001774:Blood Chemical Analysis; D002417:Cattle; D004912:Erythrocytes; D005914:Globins / Q000737:chemistry; D007501:Iron / Q000097:blood; D009753:Nutritive Value; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005914:Globins; D007501:Iron