Mouse monoclonal antibodies against Phytolacca americana antiviral protein PAP I.
Four hybridoma lines are constructed producing monoclonal antibodies against the pokeweed (Phytolacca americana) antiviral protein PAP I. Two of the antibodies, 4E8 and 5D3, are characterized in more detail. They recognize amino acid sequences rather than conformational changes and their epitopes are 65% distinct. One of these antibodies (5D3) is used to study localization of recombinant PAP I in Escherichia coli cells by immuno-gold electron microscopy.
Kaloyanova
D
D
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Kyurkchiev
S
S
Xu
J
J
Abouhaidar
M
M
Ivanov
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D000998:Antiviral Agents / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology*; D054789:Ribosome Inactivating Proteins, Type 1; D014169:Transformation, Bacterial
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein