Monoclonal antibodies against the Androctonus australis hector scorpion neurotoxin I: characterisation and use for venom neutralisation.
A series of monoclonal antibodies (mAbs) specific for the alpha-neurotoxin I (Aah I) from the venom of the dangerous Androctonus australis hector scorpion were obtained using carrier protein-coupled toxin. Competitive RIA, receptor assays and mouse toxicity tests were performed to characterise mAbs in terms of affinity and neutralisation. Cross-reactivity studies and two-site ELISA results allowed some classification of mAbs into three groups. One mAb, 9C2, was particularly interesting since it recognised the parent toxin I with a K(D) of 0.15 nM and was also reactive with toxins of the same immunological group. Its ability to neutralise the toxic effect of the parent toxin and the venom fraction has been investigated. This anti-Aah I mAb 9C2, associated with anti-Aah II mAb 4C1, provides a valuable tool to neutralise the toxicity of the venom.
Clot-Faybesse
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CNRS UMR 6560, Ingénierie des Protéines, IFR Jean Roche, Université Méditerranée, Faculté de Médecine-Nord, BD P. Dramard, Marseille, France.
Juin
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Rochat
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Devaux
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D000992:Antitoxins / Q000276:immunology / Q000627:therapeutic use; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology; D018604:Epitope Mapping; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000276:immunology* / Q000633:toxicity; D009500:Neutralization Tests; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D030162:Reptilian Proteins; D012604:Scorpion Venoms / Q000276:immunology* / Q000633:toxicity; D015222:Sodium Channels / Q000276:immunology / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000992:Antitoxins; D009498:Neurotoxins; D030162:Reptilian Proteins; D012604:Scorpion Venoms; D015222:Sodium Channels; C065314:scorpion toxin I, Androctonus; C053969:scorpion toxin II, Androctonus