Reversed phase ion-pair liquid chromatographic determination of nicotine in commercial tobacco products. 1. Moist snuff.
The official methods for the determination of nicotine in commercial tobacco products (AOAC, CORESTA) are based on approaches that are not selective for nicotine (colorimetric measurement, steam distillation, perchloric acid titration), and the availability of published methods based on state-of-the-art chromatographic methods is limited. Reversed phase ion-pair liquid chromatography has been established as a viable alternative for the analysis of basic analytes. A reversed phase ion-pair liquid chromatographic method for the determination of nicotine in commercial tobacco products was developed and optimized in separate experiments (Ciolino, L. A.; Turner, J. A.; McCauley, H. A.; Smallwood, A. W.; Yi, T. Y. J. Chromatogr. 1999a, 852 (2), 451-463). An extensive within-laboratory performance study of the optimized method was subsequently conducted, and results are presented here for the determination of nicotine in commercial moist snuff. Results for the determination of nicotine in commercial cigarettes are presented in a subsequent paper.
Ciolino
L A
LA
Forensic Chemistry Center, Food and Drug Administration, 1141 Central Parkway, Cincinnati, Ohio 45202, USA. lciolino@ora.fda.gov
McCauley
H A
HA
Fraser
D B
DB
Barnett
D Y
DY
Yi
T Y
TY
Turner
J A
JA
D002853:Chromatography, Liquid / Q000379:methods; D007202:Indicators and Reagents; D009538:Nicotine / Q000032:analysis*; D010947:Plants, Toxic; D014030:Tobacco, Smokeless / Q000737:chemistry*
D007202:Indicators and Reagents; D009538:Nicotine