title: Thermodynamics of single mismatches in RNA duplexes.
abstract: The thermodynamic properties and structures of single mismatches in short RNA duplexes were studied in optical melting and imino proton NMR experiments. The free energy increments at 37 degrees C measured for non-GU single mismatches range from -2.6 to 1.7 kcal/mol. These increments depend on the identity of the mismatch, adjacent base pairs, and the position in the helix. UU and AA mismatches are more stable close to a helix end, but GG mismatch stability is essentially unaffected by the position in the helix. Approximations are suggested for predicting stabilities of single mismatches in short RNA duplexes.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Noskowskiego.
authors.forename: R
authors.initials: R
authors.lastname: Kierzek
authors.forename: M E
authors.initials: ME
authors.lastname: Burkard
authors.forename: D H
authors.initials: DH
authors.lastname: Turner
mesh_terms: D000225:Adenine / Q000737:chemistry; D020137:Base Pair Mismatch; D020029:Base Pairing; D001483:Base Sequence; D006147:Guanine / Q000737:chemistry; D006358:Hot Temperature; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000737:chemistry; D013816:Thermodynamics; D014498:Uracil / Q000737:chemistry
chemical_list: D009692:Nucleic Acid Heteroduplexes; D012335:RNA, Ribosomal; D014498:Uracil; D006147:Guanine; D012313:RNA; D000225:Adenine