Changes of beta-1,4-galactosyltransferase with the development of endometrial cancer.
To elucidate the mechanism of the type 2 carbohydrate chain accompanying the development of endometrial cancer, we studied the expression of beta-1,4-galactosyltransferase (beta-1,4GT) in normal endometrial and endometrial cancer tissues. An immunohistochemical study revealed that beta-1,4GT was diffusely positive in the cytoplasm of endometrial cancer cells, and that the level of its expression was increased compared with that in normal endometrium. Also, beta-1,4GT mRNA corresponding to 4.7 kb was very low in normal endometrium, while an intense signal was detected in endometrial cancer. Our results suggest that the increase of beta-1,4GT contributes to the expression of the type 2 carbohydrate chain in endometrial cancer.
Kubushiro
K
K
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Ma
J
J
Fukuchi
T
T
Banno
K
K
Muramatsu
Y
Y
Tsukazaki
K
K
Nozawa
S
S
D016889:Endometrial Neoplasms / Q000201:enzymology*; D004717:Endometrium / Q000201:enzymology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D009237:N-Acetyllactosamine Synthase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D009237:N-Acetyllactosamine Synthase