Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens britton.
Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS-PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO(4) oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC(50) = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants.
Asada
M
M
Cell Biology Laboratory, Biosignalling Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan.
Fukumori
Y
Y
Inoue
M
M
Nakagomi
K
K
Sugie
M
M
Fujita
Y
Y
Tomizuka
N
N
Yamazaki
Y
Y
Oka
S
S
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D019686:Lamiaceae / Q000737:chemistry*; D008407:Mast Cells / Q000187:drug effects / Q000648:ultrastructure; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
D004791:Enzyme Inhibitors; D006023:Glycoproteins; D010936:Plant Extracts; D011493:Protein Kinase C; D006821:Hyaluronoglucosaminidase