title: Tyrosine phosphorylation in type-1 diabetes by immunogold detection: an in vitro human aortic endothelial cell (HAEC) study in the presence of diabetic low density lipoproteins (LDL).
abstract: An immunomorphometric study of tyrosine phosphorylation was performed by the immunogold technique on cultured human aortic endothelial cells (HAEC) with a view to demonstrating their impaired signal transduction status, induced in vitro by incubation with low-density lipoproteins from the plasma of Type-1 diabetic patients. The results seem to sustain the hypothesis that extranuclear bioenergetic derangement induced by low-density lipoproteins from Type-1 diabetic patients may be associated with an up-regulation of the nuclear energetic machinery aimed at maintaining intracellular metabolic equilibrium. Our data demonstrate that phosphorylated tyrosine is a useful marker to monitor this metabolic condition.
authors.affiliation: Istituto di Morfologia Umana Normale, Università di Ancona, Torrette, Italia.
authors.forename: A
authors.initials: A
authors.lastname: Pugnaloni
authors.forename: M
authors.initials: M
authors.lastname: Tesei
authors.forename: S
authors.initials: S
authors.lastname: Amati
authors.forename: L
authors.initials: L
authors.lastname: Mazzanti
authors.forename: M
authors.initials: M
authors.lastname: D'Aurelio
authors.forename: R A
authors.initials: RA
authors.lastname: Rabini
authors.forename: G
authors.initials: G
authors.lastname: Sgarbi
authors.forename: G
authors.initials: G
authors.lastname: Biagini
mesh_terms: D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008054:Lipid Peroxides / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D009687:Nuclear Proteins; D014443:Tyrosine