title: Membrane proteins of Aedes aegypti larvae bind toxins Cry4B and Cry11A of Bacillus thuringiensis ssp. israelensis.
abstract: Proteins of molecular weight 65 and 62 kD and having affinity for toxins Cry4B and Cry11A produced by Bacillus thuringiensis ssp. israelensis have been isolated from brush border membranes of Aedes aegypti larvae using affinity chromatography. Using a ligand blotting technique, we show that the binding of these proteins to the biotinylated toxins is reversible and that the two toxins compete for binding to the two proteins. These proteins are likely to be Cry4B and Cry11A toxin receptors in gut epithelial cells of Aedes aegypti larvae.
authors.affiliation: Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545, Russia. lab11@vnigen.msk.su
authors.forename: I V
authors.initials: IV
authors.lastname: Krieger
authors.forename: L P
authors.initials: LP
authors.lastname: Revina
authors.forename: L I
authors.initials: LI
authors.lastname: Kostina
authors.forename: A A
authors.initials: AA
authors.lastname: Buzdin
authors.forename: I A
authors.initials: IA
authors.lastname: Zalunin
authors.forename: G G
authors.initials: GG
authors.lastname: Chestukhina
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000330:Aedes / Q000254:growth & development*; D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins; D007814:Larva / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008565:Membrane Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis