Increased baculovirus susceptibility of armyworm larvae feeding on transgenic rice plants expressing an entomopoxvirus gene.
We have introduced an entomopoxvirus gene encoding a virus enhancing factor (EF) into rice, which resulted in high-level accumulation of the EF in the transgenic plants. The introduced gene was stably inherited in the progeny of the primary transformants, as shown by analysis of their genomic DNA. Bioassays for insect susceptibility to baculovirus infection showed that armyworm larvae feeding on the transgenic rice had increased susceptibility to a Nucleopolyhedrovirus. Thus, introduction of the EF gene into plants can be used as a strategy to increase the effectiveness of baculoviruses in insect pest management.
Hukuhara
T
T
College of Bioresource Sciences, Nihon University, Kameino, Fujisawa 252-8510, Japan. tosi@brs.nihon-u.ac.jp
Hayakawa
T
T
Wijonarko
A
A
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D017996:Entomopoxvirinae / Q000235:genetics*; D005822:Genetic Vectors; D007313:Insecta / Q000502:physiology / Q000821:virology*; D007814:Larva / Q000502:physiology / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D012275:Oryza / Q000235:genetics*; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000235:genetics; D019076:Transgenes; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins