Man-made mineral fibers induce apoptosis of human peripheral blood mononuclear cells similarly to chrysotile B.
The aim of this study was to investigate whether man-made mineral fibers (MMMF) induce apoptosis of human peripheral blood mononuclear cells (PBMC), as we recently demonstrated for chrysotile B. In vitro cultivation of PBMC with various MMMF as well as chrysotile B clearly produced apoptotic cells. The alteration of the expression for apoptosis related genes at the mRNA level during in vitro cultures of PBMC with various MMMF revealed upregulation of Flice and Apaf-1 genes and down regulation of TNF receptor 1 and Bid genes. These results indicate that MMMF induce apoptosis of PBMC in a similar manner to chrysotile B. However, the process may be mediated not only by the Fas-related apoptotic pathway but also a mitochondrial pathway. Thus, one should be aware that respiratory and immunological abnormalities may occur in workers who are exposed to MMMFs.
Ma
Z
Z
Department of Hygiene, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan.
Otsuki
T
T
Tomokuni
A
A
Aikoh
T
T
Matsuki
T
T
Sakaguchi
H
H
Isozaki
Y
Y
Hyodoh
F
F
Uehira
K
K
Isoda
K
K
Ueki
A
A
D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D017632:Asbestos, Serpentine / Q000494:pharmacology*; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D019016:Mineral Fibers / Q000633:toxicity*; D012333:RNA, Messenger / Q000096:biosynthesis; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics; D047888:Receptors, Tumor Necrosis Factor, Type I; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014452:Ubiquitins / Q000096:biosynthesis / Q000235:genetics
D015703:Antigens, CD; D017632:Asbestos, Serpentine; D018076:DNA, Complementary; D019016:Mineral Fibers; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D014452:Ubiquitins; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases