title: Do mature pulmonary lobes grow after transplantation into an immature recipient?
abstract: BACKGROUND
  The use of reduced-size adult lung transplants could help solve the profound pediatric donor lung shortage. However, adequate long-term function of the mature grafts requires growth in proportion to the recipient's development.
  
  
  METHODS
  Mature left lower lobes from adult mini-pigs (age: 7 months; mean body weight: 30 kg) were transplanted into 14-week-old piglets (mean body weight: 15 kg). By the end of the 14-week holding period, lungs of the recipients (n = 4) were harvested. After volumetric measurements, the lung morphology was studied using light microscopy, scanning, and transmission electron microscopy. Changes of alveolar airspace volume were determined using a computer aided image analysis system. Comparisons were made to age- and weight-matched controls.
  
  
  RESULTS
  Volumetric studies showed no significant differences (p = 0.49) between the specific volume (mL/kg body weight) of lobar grafts and left lower lobes of adult controls. Morphologic studies showed marked structural differences between the grafts and the right native lungs of the recipients, with increased average alveolar diameter of the grafts. On light microscopy and scanning electron microscopy, alveoli appeared dilated and rounded compared to the normal polygonal shape in the controls. The computer generated semi-quantitative data of relative alveolar airspace volume tended to be higher in transplanted lobes.
  
  
  CONCLUSIONS
  The mature pulmonary lobar grafts have filled the growing left hemithorax of the developing recipient. Emphysema-like alterations of the grafts were observed without evidence of alveolar growth in the mature lobar transplants. Thus, it can be questioned whether mature pulmonary grafts can guarantee sufficient long-term gas exchange in growing recipients.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Hannover Medical School, Germany.
authors.forename: L F
authors.initials: LF
authors.lastname: Duebener
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: S A
authors.initials: SA
authors.lastname: Tschanz
authors.forename: P H
authors.initials: PH
authors.lastname: Burri
authors.forename: H J
authors.initials: HJ
authors.lastname: Schäfers
mesh_terms: D000367:Age Factors; D000818:Animals; D015824:Blood-Air Barrier / Q000502:physiology; D005260:Female; D008168:Lung / Q000254:growth & development* / Q000473:pathology; D016040:Lung Transplantation / Q000473:pathology / Q000502:physiology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011013:Pneumonectomy; D011650:Pulmonary Alveoli / Q000473:pathology; D013552:Swine; D013556:Swine, Miniature