title: Naive, effector, and memory CD8 T cells in protection against pulmonary influenza virus infection: homing properties rather than initial frequencies are crucial.
abstract: The goal of adoptive immunotherapy is to target a high number of persisting effector cells to the site of a virus infection or tumor. In this study, we compared the protective value of hemagglutinin peptide-specific CD8 T cells generated from the clone-4 TCR-transgenic mice, defined by different stages of their differentiation, against lethal pulmonary influenza infection. We show that the adoptive transfer of high numbers of Ag-specific unprimed, naive CD8 T cells failed to clear the pulmonary virus titer and to promote host survival. The same numbers of in vitro generated primary Ag-specific Tc1 effector cells, producing high amounts of IFN-gamma, or resting Tc1 memory cells, generated from these effectors, were protective. Highly activated CD62Llow Tc1 effectors accumulated in the lung with rapid kinetics and most efficiently reduced the pulmonary viral titer early during infection. The resting CD62Lhigh naive and memory populations first increased in cell numbers in the draining lymph nodes. Subsequently, memory cells accumulated more rapidly and to a greater extent in the lung lavage as compared with naive cells. Thus, effector cells are most effective against a localized virus infection, which correlates with their ability to rapidly distribute at the infected tissue site. The finding that similar numbers of naive Ag-specific CD8 T cells are not protective supports the view that qualitative differences between the two resting populations, the naive and the memory population, may play a major role in their protective value against disease.
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA. cerwenka@dnax.org
authors.forename: A
authors.initials: A
authors.lastname: Cerwenka
authors.forename: T M
authors.initials: TM
authors.lastname: Morgan
authors.forename: R W
authors.initials: RW
authors.lastname: Dutton
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D007156:Immunologic Memory; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007399:Interphase / Q000276:immunology; D007700:Kinetics; D019041:L-Selectin / Q000096:biosynthesis; D008168:Lung / Q000821:virology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control* / Q000821:virology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000821:virology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D018984:Epitopes, T-Lymphocyte; D018960:Hyaluronan Receptors; D019041:L-Selectin; D007371:Interferon-gamma