Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, berberine, coptisine, palmatine, jateorrhizine and glycyrrhizin in Kampo medicines by ion-pair high-performance liquid chromatography.
An ion-pair high-performance liquid chromatographic method for the simultaneous determination of four flavonoids, namely baicalin, wogonoside, baicalein and wogonin, and four berberine-type alkaloids, namely berberine, coptisine, palmatine and jateorrhizine, and glycyrrhizin in Kampo medicines is described. The analysis can be accomplished within 30 min with a Wakosil-II 5C18 HG column by linear gradient elution using a mobile phase containing aqueous phosphoric acid, sodium dodecyl sulfate and acetonitrile at a flow-rate of 1.0 ml x min(-1), a thermostatic oven at 45 degrees C, and detection at 265 nm. The method was applied to quantifying these components in three Kampo decoctions: Oren-gedoku-to, San'o-shashin-to and Hange-shashin-to. The decoctions were diluted with 65% methanol at the final stage because a large quantity of precipitate, mainly from baicalin and berberine, was formed. The within-day relative standard deviations were less than 2.02% (n=10). The recoveries of these compounds were 90.3-102%. The detection limits of these compounds were 0.02-1.96 microM per injection (5 microl).
Okamura
N
N
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
Miki
H
H
Ishida
S
S
Ono
H
H
Yano
A
A
Tanaka
T
T
Ono
Y
Y
Yagi
A
A
D000470:Alkaloids / Q000032:analysis*; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis*; D019695:Glycyrrhizic Acid / Q000032:analysis; D020835:Medicine, Kampo
D000470:Alkaloids; D001600:Berberine Alkaloids; D044950:Flavanones; D005419:Flavonoids; C034384:coptisine; D001599:Berberine; C038044:baicalin; C006680:baicalein; D019695:Glycyrrhizic Acid; C005413:palmatine; C085514:wogonin