The autoregulatory HspR repressor protein governs chaperone gene transcription in Helicobacter pylori.
In the present study, we provide evidence that the groESL, hrcA-grpE-dnaK and cbpA-hspR-orf operons encoding the major chaperones of the human gastric pathogen Helicobacter pylori are transcribed by the vegetative sigma factor sigma80 and are regulated negatively by the transcriptional repressor HspR. In vitro studies with purified recombinant HspR protein established that the protein represses transcription by binding to large DNA regions centred around the transcription initiation site in the case of the Pcbp promoter, and around -85 and -120 in the case of the Pgro and Phrc promoters respectively. All three binding sites contain DNA motifs with some similarity to the HAIR sequence identified as a consensus for the HspR protein of Streptomyces. In contrast to the situation in Streptomyces, in which transcription of HspR-regulated genes is induced in response to heat shock, transcription of the HspR-dependent genes in H. pylori is not inducible by thermal stimuli. Transcription of the groESL and cbpA-hspR-orf operons is induced by osmotic shock, while transcription of the hrcA-grpE-dnaK operon, although HspR dependent, is not affected by salt treatment. The possibility that HspR could constitute a global transcriptional regulator for diverse cellular functions with implications for pathogenesis is discussed.
Spohn
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G
Department of Molecular Biology, IRIS, Chiron SpA, Via Fiorentina 1, 53100 Siena, Italy.
Scarlato
V
V
D001426:Bacterial Proteins; D001483:Base Sequence; D018834:Chaperonin 60 / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000502:physiology*; D016480:Helicobacter pylori / Q000235:genetics*; D018832:Molecular Chaperones / Q000235:genetics*; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000502:physiology; D014158:Transcription, Genetic; D014510:Urease / Q000378:metabolism
D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; C108444:HspR protein, bacteria; D018832:Molecular Chaperones; D012097:Repressor Proteins; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D014510:Urease