Goalpha and diacylglycerol kinase negatively regulate the Gqalpha pathway in C. elegans.
We investigated the EGL-30 (Gqalpha) pathway in C. elegans by using genetic screens to identify genes that confer phenotypes similar to egl-30 mutants. One such gene, egl-8, encodes a phospholipase Cbeta that is present throughout the nervous system and near intestinal cell junctions. EGL-30 and EGL-8 appear to positively regulate synaptic transmission because reducing their function results in strong aldicarb resistance and slow locomotion rates. In contrast, GOA-1 (Goalpha) and DGK-1 (diacylglycerol kinase) appear to negatively regulate synaptic transmission, because reducing their function results in strong aldicarb hypersensitivity and hyperactive locomotion. A genetic analysis suggests that GOA-1 negatively regulates the EGL-30 pathway and that DGK-1 antagonizes the EGL-30 pathway.
Miller
K G
KG
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Emerson
M D
MD
Rand
J B
JB
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D019852:Diacylglycerol Kinase / Q000502:physiology*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D015801:Helminth Proteins / Q000235:genetics / Q000502:physiology; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D009420:Nervous System / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D054799:Phospholipase C beta; D009435:Synaptic Transmission / Q000502:physiology; D010738:Type C Phospholipases / Q000235:genetics
D015801:Helminth Proteins; D007527:Isoenzymes; D019852:Diacylglycerol Kinase; D010738:Type C Phospholipases; D054799:Phospholipase C beta; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D020962:Heterotrimeric GTP-Binding Proteins