title: Molecular cloning and characterization of the Helicobacter pylori fliD gene, an essential factor in flagellar structure and motility.
abstract: Helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. Flagellar motility is regarded as a major factor in the colonizing ability of H. pylori. The functional roles of flagellar structural proteins other than FlaA, FlaB, and FlgE are not well understood. The fliD operon of H. pylori consists of flaG, fliD, and fliS genes, in the order stated, under the control of a sigma(28)-dependent promoter. In an effort to elucidate the function of the FliD protein, a hook-associated protein 2 homologue, in flagellar morphogenesis and motility, the fliD gene (2,058 bp) was cloned and isogenic mutants were constructed by disruption of the fliD gene with a kanamycin resistance cassette and electroporation-mediated allelic-exchange mutagenesis. In the fliD mutant, morphologically abnormal flagellar appendages in which very little filament elongation was apparent were observed. The fliD mutant strain was completely nonmotile, indicating that these abnormal flagella were functionally defective. Furthermore, the isogenic fliD mutant of H. pylori SS1, a mouse-adapted strain, was not able to colonize the gastric mucosae of host mice. These results suggest that H. pylori FliD is an essential element in the assembly of the functional flagella that are required for colonization of the gastric mucosa.
authors.affiliation: Mogam Biotechnology Research Institute, Koosung-myon, Yongin-city, Kyonggi-do 449-910, Korea. jsyum@kgcc.co.kr
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Chang
authors.forename: S I
authors.initials: SI
authors.lastname: Chung
authors.forename: J S
authors.initials: JS
authors.lastname: Yum
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004381:Duodenal Ulcer / Q000382:microbiology; D018274:Electroporation; D005407:Flagella / Q000737:chemistry* / Q000502:physiology; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin; C076733:FlaD protein, Bacteria