Saccharomyces cerevisiae cultured under aerobic and anaerobic conditions: air-level oxygen stress and protection against stress.
Cells of Saccharomyces cerevisiae were grown aerobically and anaerobically, and levels of the protective compounds, cysteine and glutathione, and activities of defensive enzymes, catalase and superoxide dismutase, against an oxygen stress were determined and compared in both cells. Aerobiosis increased both the compounds and enzyme activities. The elevated synthesis of glutathione could be associated with the increased levels of cysteine which in its turn was found to be controlled by the oxygen-dependent activation of cystathionine beta-synthase.
Ohmori
S
S
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka-1, Okayama, Japan.
Nawata
Y
Y
Kiyono
K
K
Murata
H
H
Tsuboi
S
S
Ikeda
M
M
Akagi
R
R
Morohashi
K I
KI
Ono
B
B
D000332:Aerobiosis; D000388:Air; D000693:Anaerobiosis; D015152:Blotting, Northern; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D003541:Cystathionine beta-Synthase / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004789:Enzyme Activation; D005978:Glutathione / Q000378:metabolism; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
D002374:Catalase; D013482:Superoxide Dismutase; D003541:Cystathionine beta-Synthase; D005978:Glutathione; D003545:Cysteine