Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom.
Agkisacutacin is a new fibrinogenlytic protein from Agkistrodon acutus venom. It consists of two heterologous subunits linked by an intersubunit disulfide bond. The cDNAs encoding the two chains of Agkisacutacin were cloned from a lambdagt11 cDNA library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). It is structurally related to the family of IX/X-binding protein (IX/X-bp)-like proteins and shows high similarity (alpha-70%/beta-64%) to habu IX/X-bp from Trimeresurus flavoridis, but displays distinct biological activity with direct action on fibrinogen.
Cheng
X
X
School of Life Science, University of Science and Technology of China, Hefei, Anhui, 230027, People's Republic of China.
Qian
Y
Y
Liu
Q
Q
Li
B X
BX
Zhang
M
M
Liu
J
J
D017836:Agkistrodon / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000235:genetics* / Q000302:isolation & purification* / Q000633:toxicity; D018076:DNA, Complementary / Q000235:genetics*; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D017386:Sequence Homology, Amino Acid
D000925:Anticoagulants; D003435:Crotalid Venoms; D018076:DNA, Complementary; C401844:agkisacutacin; D005340:Fibrinogen