Suppression of macrophage responses to bacterial lipopolysaccharide by a non-secretory form of secretory leukocyte protease inhibitor.
Expression of secretory leukocyte protease inhibitor (SLPI) suppresses the ability of macrophages to respond to bacterial lipopolysaccharide (LPS). Here, addition of recombinant or native SLPI to the extracellular medium was non-suppressive, while transfection with a non-secretory form of SLPI was fully suppressive, an effect overcome by treatment with interferon-gamma. A portion of the SLPI produced by untransfected macrophages was localized in the cytosol. Thus, SLPI can act intracellularly to block macrophage activation by LPS.
Zhu
J
J
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, USA.
Nathan
C
C
Ding
A
A
D000818:Animals; D002460:Cell Line; D017077:Culture Media, Conditioned; D003600:Cytosol / Q000378:metabolism; D015536:Down-Regulation; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009573:Nitrites / Q000378:metabolism; D010957:Plasmids; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D009573:Nitrites; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D012333:RNA, Messenger; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors; C506815:Slpi protein, mouse; D014409:Tumor Necrosis Factor-alpha