A general high-performance liquid chromatography-based assay for the hydrolysis of N-acyl glutamates.
A rapid and simple HPLC assay has been developed to separate and quantify N-acyl glutamates and the corresponding carboxylic acids of the acyl moiety. This method was specifically developed to assay hydrolytic activity for glutamate carboxypeptidases. Although established assays for specific substrates of such enzymes exist, they may not be amenable for examining the hydrolytic activity of new substrate probes. This assay was developed to accommodate such probes.
Myers
R S
RS
Department of Chemistry and Biochemistry, San Francisco State University, 1600 Holloway Avenue, San Francisco, California 94132, USA.
Paton
P T
PT
Dastgah
A
A
Martell
J R
JR
Berkman
C E
CE
D002268:Carboxypeptidases / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005971:Glutamates / Q000737:chemistry*; D006868:Hydrolysis
D005971:Glutamates; D002268:Carboxypeptidases; C026134:glutamate carboxypeptidase