title: ISRm10: a new insertion sequence of Sinorhizobium meliloti: nucleotide sequence and geographic distribution.
abstract: In this study the description of a new insertion sequence of Sinorhizobium meliloti, ISRm10, is reported. ISRm10 was found in the intergenic region between nodJ and nodQ of a natural isolated strain. ISRm10 was 1047 bp long and showed the typical features of the ISs belonging to the IS630-Tc1/IS3 superfamily. In particular the ISRm10 nucleotide sequence showed the highest homology (62%) with a Sphingomonas aromaticivorans IS. ISRm10 was present in 32% of the analyzed S. meliloti strains while it was not found in the reference strains of other Rhizobium species.
authors.affiliation: Dipartimento di Biologia Animale e Genetica, Università di Firenze, Via Romana 19, I-50125, Florence, Italy.
authors.forename: E G
authors.initials: EG
authors.lastname: Biondi
authors.forename: S
authors.initials: S
authors.lastname: Fancelli
authors.forename: M
authors.initials: M
authors.lastname: Bazzicalupo
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D000455:Medicago sativa / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial