[The correlation between integration of HBV X, S, Pre-S, C gene and the expression of oncogenes/tumor suppressor genes in primary hepatocellular carcinoma].
OBJECTIVE
To study the relation between the integration of HBV X, S, Pre-S and C genes and the expression of oncogenes/tumor suppressor genes in hepatocellular carcinoma(HCC).


METHODS
To prepare the Dig-HBV probes with random priming method, to detect the existing status of HBV DNA and the integration rates of HBV X, S, Pre-S and C genes by Southern blot and dot blot hybridization, and to examine the expression of oncogenes and tumor suppressor genes by immunohistochemistry in HCC.


RESULTS
The integration rates of HBV X, S, Pre-S and C genes in HCC were 87.5%(28/32), 62.5% (20/32), 62.5%(20/32) and 25.0%(8/32), respectively. The expression rates of p62myc, p21ras, p53 and p16 proteins were 81.2%(26/32), 50.0%(16/32), 43.8%(14/32), and 31.3(10/32), respectively. Statistical analysis showed that the expression of p62myc, p21ras and variant p53 proteins was obviously higher in HCC group with X gene than in free-X gene HCC group and that the expression of p62myc protein was obviously higher in HCC group with Pre-S gene than in free-Pre-S HCC group.


CONCLUSIONS
The integration of HBV X or Pre-S gene with cellular chromosome can activate myc, ras oncogenes and inactivate the p53 tumor suppressor gene.
Zhang
L
L
Shandong Medical University, Jinan.
Cao
Y
Y
Song
J
J
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005814:Genes, Viral; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D009857:Oncogenes; D015534:Trans-Activators / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein