title: Developmental expression of GLUT2 in the rat retina.
abstract: We previously demonstrated that GLUT2, a facilitated-diffusion glucose transporter isoform known to play critical roles in the regulation of systemic blood glucose level, is present at the apical ends of Müller cells in the rat retina. As a means of elucidating the ontogeny and possible role(s) of GLUT2 in the developing retina, this study examined its expression at various stages of retinal development by immunofluorescence staining using GLUT2-specific antibody. Evidence of GLUT2 expression first appeared at embryonic day 14 (E14) as linear staining along the boundary between the inner and outer layers of the optic cup, with this staining pattern being present throughout subsequent embryonic and neonatal stages. After the development of photoreceptor cell inner and outer segments (i.e., photoreceptor layer), GLUT2 immunoreactivity was localized along the boundary between the outer nuclear layer and photoreceptor layer. Localization of GLUT2 expression and the timing of its appearance, which coincided with the formation of choriocapillaries, together suggest that GLUT2 is involved in the anterior transport of glucose supplied by choroidal circulation from the early stages of retinal development.
authors.affiliation: Department of Clinical Pathology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan. ur9t-wtnb@asahi-net.or.jp
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Nagamatsu
authors.forename: S
authors.initials: S
authors.lastname: Matsushima
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: H
authors.initials: H
authors.lastname: Motobu
authors.forename: K
authors.initials: K
authors.lastname: Hirosawa
authors.forename: K
authors.initials: K
authors.lastname: Mabuchi
authors.forename: T
authors.initials: T
authors.lastname: Kirino
authors.forename: H
authors.initials: H
authors.lastname: Uchimura
mesh_terms: D000818:Animals; D002196:Capillaries / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D005455:Fluorescent Antibody Technique; D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins / Q000032:analysis*; D010857:Pigment Epithelium of Eye / Q000196:embryology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry* / Q000196:embryology* / Q000648:ultrastructure; D012171:Retinal Vessels / Q000166:cytology
chemical_list: D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins