Long-term survival of shiga toxin-producing Escherichia coli O26, O111, and O157 in bovine feces.
Cattle are an important reservoir of Shiga toxin-producing Escherichia coli (STEC) O26, O111, and O157. The fate of these pathogens in bovine feces at 5, 15, and 25 degrees C was examined. The feces of a cow naturally infected with STEC O26:H11 and two STEC-free cows were studied. STEC O26, O111, and O157 were inoculated into bovine feces at 10(1), 10(3), and 10(5) CFU/g. All three pathogens survived at 5 and 25 degrees C for 1 to 4 weeks and at 15 degrees C for 1 to 8 weeks when inoculated at the low concentration. On samples inoculated with the middle and high concentrations, O26, O111, and O157 survived at 25 degrees C for 3 to 12 weeks, at 15 degrees C for 1 to 18 weeks, and at 5 degrees C for 2 to 14 weeks, respectively. Therefore, these pathogens can survive in feces for a long time, especially at 15 degrees C. The surprising long-term survival of STEC O26, O111, and O157 in bovine feces shows that such feces are a potential vehicle for transmitting not only O157 but also O26 and O111 to cattle, food, and the environment. Appropriate handling of bovine feces is emphasized.
Fukushima
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Public Health Institute of Shimane Prefecture, Nishihamasada, Matsue, Shimane 690-0122, Japan. hiroshi@joho-shimane.or.jp
Hoshina
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Gomyoda
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D000332:Aerobiosis; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle; D003603:Cytotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000302:isolation & purification / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000502:physiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D022603:Shiga Toxins; D013045:Species Specificity; D013997:Time Factors
D001427:Bacterial Toxins; D003603:Cytotoxins; D022603:Shiga Toxins