The nuclear pore complex protein Nup88 is overexpressed in tumor cells.
It has been previously shown (J. Schneider et al., Br. J. Cancer, 77: 1015-1020, 1998) that an antibody directed against Candida albicans proteins (C6) recognizes specifically a protein in human ovarian tumors. We have now performed an immunoscreening of a human cDNA library using this antibody and identified the antigen as Nup88, a protein localized preferentially at the nuclear membrane and probably implicated in nucleocytoplasmic transport. Our results show that Nup88 is strongly expressed in a series of human tumor cell lines compared with nontransformed cell lines at the RNA and the protein levels. Furthermore, we observed that in 76% of 21 ovarian tumors analyzed, the protein is also overexpressed in malignant tissue when compared with healthy adjacent tissue. Nup88 may, therefore, be considered as a putative marker for tumor growth and is probably related to increased cell cycling.
Martínez
N
N
Deutsches Krebsforschungszentrum, 69120-Heidelberg, Germany.
Alonso
A
A
Moragues
M D
MD
Pontón
J
J
Schneider
J
J
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000235:genetics*; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D008565:Membrane Proteins; C104835:NUP88 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger