Determination of age-related changes in human soluble interleukin 2 receptor in body fluids of normal subjects as a control value against disease states.
A highly sensitive enzyme-linked immunosorbent assay (ELISA) system was developed for human soluble interleukin-2 receptor (sIL-2R) with an ELISA-amplification system (ELAST((R))). The sensitivity of the new method was 20-fold higher than that without the amplification. Thus very low concentrations of sIL-2R in urine can be detected. With this method, serum and urine concentrations of sIL-2R were analyzed for healthy Japanese subjects with age 1-67 years. Mean sIL-2R concentrations in both serum and urine of children were significantly higher than those of adults. However, the concentrations of children showed a progressive decline to those of adults by the age of 15 years. There was no difference in the values between males and females. The results provide a control value of sIL-2R against those in disease states such as nephrotic syndrome. Since the trends in serum and urine were found to be similar, urinary sIL-2R measurement may provide sufficient information, without measuring the blood concentration.
Gotoh
Y
Y
Department of Clinical Pharmacy, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Japan.
Okamoto
Y
Y
Uemura
O
O
Mori
N
N
Tanaka
S
S
Ando
T
T
Nishida
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D001826:Body Fluids / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000097:blood; D012016:Reference Values; D012680:Sensitivity and Specificity; D014556:Urine / Q000737:chemistry
D015375:Receptors, Interleukin-2