Expression of an mNSC1 in mammalian cells.
We have cloned a cDNA inducing a cation-permeable current (mNSC1) from pancreatic beta-cells, which shows niflumate-sensitive current in Xenopus oocytes. To elucidate the expression in mammalian cells, mNSC1 was expressed in CHO cells. The reversal potential by mNSC1 was shifted toward positive which was significantly reversed by flufenamic acid. Single-channel analysis showed a characteristic of a Ca-activated nonselective cation channel. Therefore, we may conclude that mNSC1 expresses a fenamates-sensitive cation channel, inducing membrane depolarization in a mammalian cell.
Miyoshi
T
T
Department of Pharmacology, Jichi Medical School, Tochigi.
Ooki
G
G
Murata
M
M
Hayakawa
H
H
Tomita
K
K
Imai
M
M
Suzuki
M
M
D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D005439:Flufenamic Acid / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D008322:Mammals; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014982:Xenopus laevis
D018076:DNA, Complementary; D007473:Ion Channels; D011994:Recombinant Proteins; D005439:Flufenamic Acid