Polymerization of diaspirin cross-linked hemoglobin (DCLHb) with water-soluble, nonimmunogenic polyamide cross-linking agents.
Diaspirin cross-linked hemoglobin (DCLHb), a human hemoglobin that is intramolecularly cross-linked between the alpha chains (lysine 99(alpha)(1)-lysine 99(alpha)(2)), was polymerized with a number of water-soluble, nonimmunogenic polyamide cross-linking agents. The degree of polymerization and the oxygen-carrying capacity depended upon the polyamide reagent, the starting concentration of DCLHb, the molar ratio of the polyamide reagent to DCLHb used, the reaction pH, and whether oxy- or deoxy-DCLHb was used in the polymerization reaction.
Hai
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TT
Corporate Research and Technical Services, Baxter Healthcare Corporation, Round Lake, Illinois 60073-9799, USA. ton_hait@baxter.com
Pereira
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DE
Nelson
D J
DJ
Srnak
A
A
Catarello
J
J
D001241:Aspirin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D003432:Cross-Linking Reagents; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008970:Molecular Weight; D009757:Nylons / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012995:Solubility; D014867:Water
D003432:Cross-Linking Reagents; D006454:Hemoglobins; D007202:Indicators and Reagents; D009757:Nylons; D010108:Oxyhemoglobins; D011108:Polymers; D014867:Water; C100003:deoxyhemoglobin; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin; D010100:Oxygen