title: Accumulation of mature mRNA in the nuclear fraction of mammalian cells.
abstract: Little is known about the nuclear mRNA content of mammalian cells. In this study, we analyzed by Northern blotting with a panel of probes the nuclear and cytoplasmic fractions derived from several rodent cell lines. For most of the genes under study, mature mRNAs could easily be detected in the nuclear fraction and accumulated to higher levels than the corresponding precursors. In addition, significant differences in the nucleo-cytoplasmic partition of mature mRNAs were observed between genes as well as between cell types (NIH 3T3, CTLL-2, D3-ES, PC-12), indicating that this nuclear accumulation of mRNA is regulated. Thus, while it is usually considered that splicing is the limiting step of pre-mRNA processing, these results point towards transport or nuclear retention of mRNA as a key determinant of nuclear mRNA metabolism.
authors.affiliation: CNRS UPR 1983, Génétique Moléculaire et Intégration des Fonctions Cellulaires, Institut de Recherches sur le Cancer, Villejuif, France.
authors.forename: P
authors.initials: P
authors.lastname: Gondran
authors.forename: F
authors.initials: F
authors.lastname: Amiot
authors.forename: D
authors.initials: D
authors.lastname: Weil
authors.forename: F
authors.initials: F
authors.lastname: Dautry
mesh_terms: D000818:Animals; D001692:Biological Transport; D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry; D015342:DNA Probes; D007700:Kinetics; D051379:Mice; D015530:Nuclear Matrix / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger