Detection of endometrial cancer by flow cytometry using two monoclonal antibodies.
BACKGROUND
To develop a supplementary diagnostic method for endometrial cancer by measuring the reactivity of various endometrial lesions with two monoclonal antibodies.


METHODS
We investigated the reactivity of various endometrial lesions with two monoclonal antibodies (MSN-1 and MSN-3) by flow cytometry (one-color and two-color methods).


RESULTS
The two-color method appeared to be suitable for use in place of simultaneous performance of the one-color methods with MSN-1 and MSN-3. The positivity rate for normal endometrium was 16.0% with the two-color method, which was lower than the rate of 30.0% obtained with concomitant used of the one-color methods. The positivity rate for endometrial cancer was high, 84.0%, with the two-color method. The positivity rate was 85.7% for well-differentiated endometrial cancer, 71.4% for moderately differentiated cancer, and 100.0% for poorly differentiated cancer; thus, the rate was high irrespective of the cellular differentiation.


CONCLUSIONS
The two-color method is more useful than the one-color method as a supplementary diagnostic procedure for endometrial cancer.
Kobiki
K
K
Department of Obstetrics and Gynecology, Kawasaki Municipal Hospital, Kawasaki City, Japan. drkoby@kt.rim.or.jp
Tsukasaki
K
K
Kubushiro
K
K
Kobayashi
Y
Y
Hasegawa
K
K
Fukuchi
T
T
Banno
K
K
Nozawa
S
S
D000912:Antibodies, Neoplasm / Q000378:metabolism; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D004717:Endometrium / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor