Effect of six steroidal saponins isolated from anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood.
Six steroidal saponins were isolated from Anemarrhena asphodeloides Bunge (Liliaceae), a traditional chinese medicine, and named anemarrhenasaponin I (An-I), anemarrhenasaponin Ia (An-Ia), timosaponin B-I (TB-I), timosaponin B-II (TB-II), timosaponin B-III (TB-III), and timosaponin A-III (TA-III). The effects of these six compounds on platelet aggregation and hemolysis in human blood were studied. All these compounds provoked remarkable inhibiting effect on platelet aggregation, and activated partial thromboplastin times (APTT) are sensitive to the presence of these six compounds. Using an in vitro system, APTT was delayed with the increment of the concentrations of these six compounds. In these six compounds, only timosaponin A-III appeared a strong effect on hemolysis, and anemarrhenasaponin Ia had a slight effect on hemolysis, other had no effect on hemolysis. These results suggested that these steroidal saponins isolated from Anemarrhena asphodeloides Bunge (Liliaceae) might be used as a novel antithrombotic therapeutic agents in post-myocardial infarction.
Zhang
J
J
Division of Oral Biology, School of Dental Medicine, The University of Pittsburgh, Room 589, Salk Hall, 3501 Terrace Street, Pittsburg, PA 15261, USA.
Meng
Z
Z
Zhang
M
M
Ma
D
D
Xu
S
S
Kodama
H
H
D000991:Antithrombins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure; D010314:Partial Thromboplastin Time; D010946:Plants, Medicinal / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry
D000991:Antithrombins; D010975:Platelet Aggregation Inhibitors; D012503:Saponins; D013256:Steroids