PCNA/AgNOR and Ki-67/AgNOR double staining in oral squamous cell carcinoma.
This study was performed on oral squamous cell carcinomas (OSCC) in order to investigate the relation between the number of interphase silver-stained nucleolar organizer regions (AgNORs) and the immunolabeling of proliferation-associated markers, using antibodies to Ki-67 and proliferating cell nuclear antigen (PCNA). Fifteen consecutive cases of oral squamous cell carcinoma were used and a double staining technique was performed in order to quantify the number of NORs in PCNA-positive and -negative cells as well as in Ki-67-positive and -negative cells. Our results showed a higher mean number of AgNORs in PCNA- and Ki-67-positive cells than in PCNA- and Ki-67-negative cells. We concluded that there is an association between cell proliferation and AgNOR score in OSCC.
Costa
A de L
Ade L
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Norte, Natal, Brazil.
de Araújo
N S
NS
Pinto
D dos S
Ddos S
de Araújo
V C
VC
D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009060:Mouth Floor; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism*; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D016622:Silver Staining / Q000379:methods* / Q000706:statistics & numerical data
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen