Translocation of a calcium-permeable cation channel induced by insulin-like growth factor-I.
Calcium plays a critical part in the regulation of cell growth, and growth factors stimulate calcium entry into cells through calcium-permeable channels. However, the molecular nature and regulation of calcium-permeable channels are still unclear at present. Here we report the molecular characterization of a calcium-permeable cation channel that is regulated by insulin-like growth factor-I (IGF-I). This channel, which we name growth-factor-regulated channel (GRC), belongs to the TRP-channel family and localizes mainly to intracellular pools under basal conditions. Upon stimulation of cells by IGF-I, GRC translocates to the plasma membrane. Thus, IGF-I augments calcium entry through GRC by regulating trafficking of the channel.
Kanzaki
M
M
Department of Cell Biology, Gunma University, Maebashi, Japan.
Zhang
Y Q
YQ
Mashima
H
H
Li
L
L
Shibata
H
H
Kojima
I
I
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology*; D002867:Chromones / Q000494:pharmacology; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010802:Phylogeny; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D050916:TRPV Cation Channels; D014162:Transfection; D000077191:Wortmannin
D000730:Androstadienes; D015220:Calcium Channels; D002867:Chromones; D009025:Morpholines; D011993:Recombinant Fusion Proteins; D050916:TRPV Cation Channels; C400769:TRPV2 protein, human; C485717:Trpv2 protein, mouse; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D007334:Insulin-Like Growth Factor I; D019869:Phosphatidylinositol 3-Kinases; D002118:Calcium; D000077191:Wortmannin