Absence of L-arginine effect on coronary hypersensitivity to serotonin in cardiac transplant recipients.
Coronary hypersensitivity to serotonin promotes platelet aggregation and, therefore, the progression of the atherosclerotic process. This abnormality occurs in the early stages of coronary atherosclerosis when the responses to bradykinin are still preserved. To determine whether such changes also occur early after cardiac transplantation, intracoronary injections of bradykinin and serotonin were performed in 7 control patients, in 19 patients with dyslipidemia, and in 15 cardiac transplant recipients (1 year after operation). Coronary angiography was normal in the 3 groups. In the segments where serotonin effects were the most pronounced, the diameter changes were measured by quantitative angiography. Bradykinin (60, 200, and 600 ng) increased in the same way as the coronary diameters in the 3 groups; in contrast, serotonin elicited vasodilation only in the control group (7+/-3%, percentage of baseline) and vasoconstriction in the hyperlipidemic group (-9+/-2%) and in transplant recipients (-15+/-3%). After intracoronary infusion of L-arginine (40 mg/min for 14 minutes), serotonin-induced constriction was attenuated in the hyperlipidemic group but not in transplant recipients. Thus, the response to bradykinin is preserved in the early stages of graft vasculopathy. However, in contrast to patients with hyperlipidemia, the absence of an L-arginine effect on the responses to serotonin suggests the involvement of mechanisms other than a decrease in endothelium-derived nitric oxide availability. Immune processes promoting the release of endothelium-derived contracting factors such as endothelin and/or superoxide anion may play a role.
Berkenboom
G
G
Department of Cardiology, Erasme Hospital, Free University of Brussels, Belgium. gberkenb@ulb.ac.be
Crasset
V
V
Unger
P
P
Vachiery
J L
JL
LeClerc
J L
JL
D001120:Arginine / Q000502:physiology*; D017023:Coronary Angiography; D004730:Endothelium, Vascular / Q000000981:diagnostic imaging / Q000503:physiopathology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D016027:Heart Transplantation / Q000502:physiology*; D006439:Hemodynamics; D006801:Humans; D006949:Hyperlipidemias / Q000503:physiopathology; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
D016166:Free Radical Scavengers; D012701:Serotonin; D001120:Arginine; D019001:Nitric Oxide Synthase