Expression of gamma-glutamylcysteine synthetase mRNA in regenerating rat liver.
Changes in the level of GSH and gamma-Glutamylcysteine Synthetase (gamma-GCS) activity with time were determined during regeneration of rat liver after partial hepatectomy. The GSH level and gamma-GCS activity increased in regenerating rat liver, reaching a maximum (2.1- and 1.5-fold that in sham-operated controls) on day 2, respectively. Furthermore, gamma-GCS mRNA expression increased within 24 h and reached maximum on day 2. These results suggest that the increased GSH level in regenerating rat liver after partial hepatectomy is associated with the transcriptional activation of gamma-GCS gene and the sequence elevation in gamma-GCS activity.
Tsuboi
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
D000818:Animals; D005721:Glutamate-Cysteine Ligase / Q000096:biosynthesis* / Q000235:genetics; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology* / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D015533:Transcriptional Activation
D012333:RNA, Messenger; D005721:Glutamate-Cysteine Ligase