Reversed-phase high-performance liquid chromatographic measurement of the P-aminobenzoic acid synthesized by Streptococcus sanguis.
OBJECTIVE
This study investigated the biosynthesis of P-aminobenzoic acid by Streptococcus sanguis and the role of P-aminobenzoic acid in the interaction between Streptococcus sanguis and Streptococcus mutans.


METHODS
A reversed-phase high-performance liquid chromatographic technique was used to analyze the P-aminobenzoic acid synthesized by Streptococcus sanguis in an anaerobic atmosphere.


RESULTS
The results showed, that (1) the chromatographic method established in this study had good linear relation and the average recovery of P-aminobenzoic acid was 80%, and (2) Streptococcus sanguis did synthesize P-aminobenzoic acid, and the mean concentration of P-aminobenzoic acid was 1.23 micrograms/mL.


CONCLUSIONS
The results of this study will help further studies of the factors that may have effects on P-aminobenzoic acid synthesis by Streptococcus sanguis and on the role of P-aminobenzoic acid in the microbial homeostasis of dental plaque.
Zhou
X D
XD
College of Stomatology, West China University of Medical Sciences, Chengdu, P. R. China.
Hu
T
T
Zhang
P
P
Tan
H
H
D010129:4-Aminobenzoic Acid / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003773:Dental Plaque / Q000382:microbiology*; D017753:Ecosystem; D013295:Streptococcus mutans / Q000378:metabolism; D013298:Streptococcus sanguis / Q000378:metabolism*
D010129:4-Aminobenzoic Acid