title: Heterogeneity of NK1.1+ T cells in the bone marrow: divergence from the thymus.
abstract: NK1.1+ T cells in the mouse thymus and bone marrow were compared because some marrow NK1.1+ T cells have been reported to be extrathymically derived. Almost all NK1.1+ T cells in the thymus were depleted in the CD1-/-, beta2m-/-, and Jalpha281-/- mice as compared with wild-type mice. CD8+NK1.1+ T cells were not clearly detected, even in the wild-type mice. In bone marrow from the wild-type mice, CD8+NK1.1+ T cells were easily detected, about twice as numerous as CD4+NK1.1+ T cells, and were similar in number to CD4-CD8-NK1.1+ T cells. All three marrow NK1.1+ T cell subsets were reduced about 4-fold in CD1-/- mice. No reduction was observed in CD8+NK1.1+ T cells in the bone marrow of Jalpha281-/- mice, but marrow CD8+NK1.1+ T cells were markedly depleted in beta2m-/- mice. All NK1.1+ T cell subsets in the marrow of wild-type mice produced high levels of IFN-gamma, IL-4, and IL-10. Although the numbers of marrow CD4-CD8-NK1.1+ T cells in beta2m-/- and Jalpha281-/- mice were similar to those in wild-type mice, these cells had a Th1-like pattern (high IFN-gamma, and low IL-4 and IL-10). In conclusion, the large majority of NK1.1+ T cells in the bone marrow are CD1 dependent. Marrow NK1.1+ T cells include CD8+, Valpha14-Jalpha281-, and beta2m-independent subsets that are not clearly detected in the thymus.
authors.affiliation: Division of Immunology and Rheumatology, Department of Medicine, Stanford Medical School, CA 94305, USA.
authors.forename: D
authors.initials: D
authors.lastname: Zeng
authors.forename: G
authors.initials: G
authors.lastname: Gazit
authors.forename: S
authors.initials: S
authors.lastname: Dejbakhsh-Jones
authors.forename: S P
authors.initials: SP
authors.lastname: Balk
authors.forename: S
authors.initials: S
authors.lastname: Snapper
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: S
authors.initials: S
authors.lastname: Strober
mesh_terms: D000818:Animals; D000941:Antigens / Q000096:biosynthesis*; D018949:Antigens, CD1 / Q000235:genetics; D000950:Antigens, Ly; D000954:Antigens, Surface; D001854:Bone Marrow Cells / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D019673:Genes, T-Cell Receptor alpha; D037181:Lectins, C-Type; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008822:Mice, Transgenic; D055651:NK Cell Lectin-Like Receptor Subfamily B; D014176:Protein Biosynthesis; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000172:deficiency / Q000235:genetics
chemical_list: D000941:Antigens; D018949:Antigens, CD1; D000950:Antigens, Ly; D000954:Antigens, Surface; D016207:Cytokines; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D001613:beta 2-Microglobulin