Glycolipid receptors for verotoxin and Helicobacter pylori: role in pathology.
Eukaryotic cell surface glycolipids can act as both the primary interface between bacteria and their host and secondly as a targeting mechanism for bacterial virulence factors. The former is characterized by redundancy in adhesin-receptor interactions and the latter by a higher affinity, more restrictive glycolipid binding specificity for targeting. Interactions of verotoxin with its glycolipid receptor globotriaosylceramide and Helicobacter pylori binding to a variety of different glycolipids, which can be environmentally regulated, provide examples of these differing modes of glycolipid receptor function. Verotoxins are involved in endothelial targeting in the microangiopathies of hemorrhagic colitis and hemolytic uremic syndrome (HUS). The highly restricted binding specificity and crystal structure of the verotoxin B subunit have allowed theoretical modeling of the Gb3 binding site of the verotoxin B subunit pentamer which provides an approach to intervention. Studies of the role of glycolipid function in verotoxin-induced disease have concentrated on the distribution of Gb3 and its ability to mediate the internalization of the toxin within the target cell. The distribution of Gb3 within the renal glomerulus plays a central role in defining the age-related etiology of HUS following gastrointestinal infection with VT producing Escherichia coli. H. pylori, on the other hand, instigates a less distinct but more complex disseminated gastric inflammation. Studies on the role of glycolipid receptors in H. pylori infection have been bogged down in establishing the importance of each binding specificity defined. In addition, the physiological condition of the organism within the various binding assays has not been extensively considered, such that spurious non-physiological interactions may have been elucidated. The identification and cloning of a Le(b) binding adhesin and the identification of cell surface hsp70 as a mediator of sulfoglycolipid binding under stress conditions may now allow a more molecular approach to define the role of glycolipid recognition in this infection.
Lingwood
C A
CA
Department of Laboratory Medicine, University of Toronto, and Research Institute, Hospital for Sick Children, ON, Canada. cling@sickkids.on.ca
D018829:Adhesins, Bacterial / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000633:toxicity*; D001665:Binding Sites; D004927:Escherichia coli Infections / Q000150:complications / Q000517:prevention & control; D005767:Gastrointestinal Diseases / Q000150:complications / Q000517:prevention & control; D016481:Helicobacter Infections / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006463:Hemolytic-Uremic Syndrome / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007678:Kidney Glomerulus / Q000382:microbiology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D022622:Shiga Toxin 1; D013270:Stomach / Q000382:microbiology; D013312:Stress, Physiological / Q000503:physiopathology; D014281:Trihexosylceramides / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001427:Bacterial Toxins; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; D014281:Trihexosylceramides; C033639:glycolipid receptor; C018549:globotriaosylceramide