Effect of hyperbaric oxygen on microvascular anastomosis healing and patency in the rat.
Recent evidence has shown that changes in blood viscosity and marked increases in both platelet count and fibrinogen concentration occur after exposure to hyperbaric oxygen (HBO). The purpose of the present study was to address whether repeated HBO therapy would increase either hematocrit or platelet count, and to determine if these changes would influence the patency of the microvascular anastomosis, as well as anastomotic healing. Eighty femoral arteries from 40 rats were divided into four groups (n = 10), and arterial anastomoses were performed on normal arteries in the first two groups, and on crushed arteries in the second two groups. One of the normal artery groups and one of the crushed artery groups received repeated HBO treatments for 5 days. Anastomotic patency rates, platelet count, hematocrit, and breaking strength were recorded. Among the results of this study were that: (1) there was no difference in patency rate following HBO treatment in both the normal and crushed artery groups; (2) platelet count was significantly higher following HBO treatment in the groups with the undamaged arteries; (3) breaking strength was significantly increased following HBO treatment in the groups with the crushed arteries. The authors concluded that HBO therapy may be useful in improving the healing of microvascular anastomoses in vessels that have undergone crush injury. Although HBO treatment results in an increased platelet count, this does not influence anastomotic patency.
Shi
D Y
DY
Microsurgical Replantation Transplantation Service, California Pacific Medical Center, San Francisco, USA.
Zhang
F
F
Kryger
Z
Z
Komorowska-Timek
E
E
Lineaweaver
W C
WC
Buncke
H J
HJ
D000714:Anastomosis, Surgical; D000818:Animals; D016009:Chi-Square Distribution; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000473:pathology / Q000601:surgery*; D006400:Hematocrit; D006931:Hyperbaric Oxygenation; D008297:Male; D008833:Microcirculation / Q000502:physiology; D010976:Platelet Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D014654:Vascular Patency; D014656:Vascular Surgical Procedures / Q000379:methods*; D014945:Wound Healing