title: Helicobacter pylori and neutrophils: sialic acid-dependent binding to various isolated glycoconjugates.
abstract: Helicobacter pylori has been shown to agglutinate erythrocytes in a sialic acid-dependent manner. However, very few studies have examined relevant target cells in the human stomach. Neutrophils are required for the onset of gastritis, and the inflammatory reaction may be induced on contact between bacteria and neutrophils. In the present work, glycolipids and glycoproteins were isolated from neutrophils and were studied for binding by overlay with radiolabeled bacteria on thin-layer chromatograms and on membrane blots. There was a complex pattern of binding bands. The only practical binding activity found was sialic acid dependent, since treatment of glycoconjugates with neuraminidase or mild periodate eliminated binding. As shown before for binding to erythrocytes and other glycoconjugates, bacterial cells grown on agar bound to many glycoconjugates, while growth in broth resulted in bacteria that would bind only to polyglycosylceramides, which are highly heterogeneous and branched poly-N-acetyllactosamine-containing glycolipids. Approximately seven positive bands were found for glycoproteins, and the traditional ganglioside fraction showed a complex, slow-moving interval with very strong sialic-acid-dependent binding, probably explained by Fuc substitutions on GlcNAc.
authors.affiliation: Institute of Medical Biochemistry, Göteborg University, SE 405 30 Göteborg, Sweden. Halina.Miller-Podraza@medkem.gu.se
authors.forename: H
authors.initials: H
authors.lastname: Miller-Podraza
authors.forename: J
authors.initials: J
authors.lastname: Bergström
authors.forename: S
authors.initials: S
authors.lastname: Teneberg
authors.forename: M A
authors.initials: MA
authors.lastname: Milh
authors.forename: M
authors.initials: M
authors.lastname: Longard
authors.forename: B M
authors.initials: BM
authors.lastname: Olsson
authors.forename: L
authors.initials: L
authors.lastname: Uggla
authors.forename: K A
authors.initials: KA
authors.lastname: Karlsson
mesh_terms: D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D005732:Gangliosides / Q000378:metabolism; D006001:Glycoconjugates / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D016480:Helicobacter pylori / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005732:Gangliosides; D006001:Glycoconjugates; D006023:Glycoproteins; D019158:N-Acetylneuraminic Acid