Eggplant tissue homogenate-based bioselective membrane electrode for determination of catechol.
A novel eggplant tissue homogenate-based membrane electrode with high selective response to catechol (5 x 10(-6)-2.5 x 10(-5) M concentration) has been constructed by immobilizing tissue of eggplant (Solanum melangena L.) at dissolved oxygen probe. In order to optimize the stability of the electrode, general immobilization techniques are used to secure the eggplant tissue section physically in a gelatin-glutaraldehyde cross-linking matrix. The electrode response was maximum when 50 mM phosphate buffer was used at pH 7.0 and 35 degrees C. The sensor is stable for more than 3 months.
Dinçkaya
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Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Turkey. erbchem@fenfak.ege.edu.tr
Akyilmaz
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Telefoncu
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Akgöl
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D015374:Biosensing Techniques; D002021:Buffers; D002396:Catechols / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D019657:Solanaceae; D013696:Temperature
D002021:Buffers; D002396:Catechols; C034221:catechol