Genotypic diversity among Brevibacillus laterosporus strains.
In comparison with other entomopathogenic Bacillus species, the genome of Brevibacillus laterosporus is poorly characterized. The aim of this study was to examine genetic variability in B. laterosporus by using a range of typing methodologies. Strains of B. laterosporus were examined for variation in 13 chromosomal genes encoding enzymes by multilocus enzyme electrophoresis. Optimal conditions of pulsed-field gel electrophoresis and randomly amplified polymorphic DNA were established that allowed analysis of the genome of B. laterosporus. None of these techniques allowed the identification of a convenient molecular marker for entomopathogenic strains, although one specific primer amplified only DNA from almost all mosquitocidal strains.
Zahner
V
V
Laboratory of Systematic Biochemistry, Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute-FIOCRUZ, Rio de Janeiro, RJ, Brazil. vzahner@gene.dbbm.fiocruz.br
Rabinovitch
L
L
Suffys
P
P
Momen
H
H
D001407:Bacillus / Q000145:classification* / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004798:Enzymes / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
D004798:Enzymes; D005819:Genetic Markers