title: Calcium- and magnesium-dependent aggregation of legume seed storage proteins.
abstract: The solubility characteristics and sedimentation behavior of total or individual globulins from legume seeds [Lupinus albus L., Pisum sativum L., and Glycine max (L.) Merr.] were investigated. The typical insolubility of globulins detected during their extraction seems to be due to the presence of a low molecular weight factor(s) in the seed extract. The solubility of the purified globulins decreases with increasing concentrations of calcium and/or magnesium, but not of other cations, showing minimum values at concentrations that vary with the particular globulin considered. Ultracentrifugation analyses revealed that the Ca(2+)- and/or Mg(2+)-induced insolubilization of the globulins involves the formation of high-order aggregates of molecules of the same or of different globulins. These macromolecular structures are dissociated under conditions of high ionic strength, suggesting the involvement of electrostatic interactions in the aggregation process. The degree of association relies heavily on the amount of Ca(2+) and/or Mg(2+) available, on the presence of chelating agents for these divalent cations, and on the ionic strength of the surrounding medium. The possible physiological significance of the findings is discussed.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal. ferreira@itqb.unl.pt
authors.forename: R B
authors.initials: RB
authors.lastname: Ferreira
authors.forename: E
authors.initials: E
authors.lastname: Franco
authors.forename: A R
authors.initials: AR
authors.lastname: Teixeira
mesh_terms: D002122:Calcium Chloride; D007887:Fabaceae / Q000737:chemistry*; D015636:Magnesium Chloride; D018532:Pisum sativum / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry*; D012995:Solubility; D013025:Glycine max / Q000737:chemistry
chemical_list: D010940:Plant Proteins; D015636:Magnesium Chloride; D002122:Calcium Chloride