An alpha2,3 sialyltransferase (ST3Gal I) is elevated in primary breast carcinomas.
The MUC1 mucin is expressed on the luminal surface of most simple epithelial cells but in carcinomas, especially those of the breast and ovary, MUC1 is upregulated and aberrantly glycosylated. MUC1 contains a large amount of O-linked glycans which, in the mucin expressed by normal mammary epithelial cells, consist mainly of core 2 based structures carrying polylactosamine chains. However, the mucin expressed by breast carcinomas has shorter side-chains, often consisting of sialylated core 1 (Galbeta1-3GalNAc). in situ hybridization of primary breast tissue showed that a sialyltransferase (ST3Gal I), responsible for adding sialic acid to core 1 thereby terminating chain extension, is elevated in primary breast carcinomas when compared to normal or benign tissue. Furthermore, the level of mRNA expression encoding ST3Gal I is correlated to the intensity of staining seen with the antibody SM3, which specifically recognises underglycosylated, tumour associated MUC1. Thus, the aberrant glycosylation of MUC1 seen in breast carcinomas appears to be due, at least in part, to the elevation of ST3Gal I.
Burchell
J
J
Imperial Cancer Research Fund Breast Cancer Biology Group, Guy's Hospital, London, UK.
Poulsom
R
R
Hanby
A
A
Whitehouse
C
C
Cooper
L
L
Clausen
H
H
Miles
D
D
Taylor-Papadimitriou
J
J
D001943:Breast Neoplasms / Q000201:enzymology*; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D007124:Immunoenzyme Techniques; D018396:Mucin-1 / Q000032:analysis / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism*; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
D018396:Mucin-1; D012333:RNA, Messenger; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase; C000722087:ST3GAL1 protein, human