c-mos and cdc2 cooperate in the translational activation of fibroblast growth factor receptor-1 during Xenopus oocyte maturation.
During oocyte maturation in Xenopus, previously quiescent maternal mRNAs are translationally activated at specific times. We hypothesized that the translational recruitment of individual messages is triggered by particular cellular events and investigated the potential for known effectors of the meiotic cell cycle to activate the translation of the FGF receptor-1 (XFGFR) maternal mRNA. We found that both c-mos and cdc2 activate the translation of XFGFR. However, although oocytes matured by injection of recombinant cdc2/cyclin B translate normal levels of XFGFR protein, c-mos depletion reduces the level of XFGFR protein induced by cdc2/cyclin B injection. In oocytes blocked for cdc2 activity, injection of mos RNA induced low levels of XFGFR protein, independent of MAPK activity. Through the use of injected reporter RNAs, we show that the XFGFR 3' untranslated region inhibitory element is completely derepressed by cdc2 alone. In addition, we identified a new inhibitory element through which both mos and cdc2 activate translation. We found that cdc2 derepresses translation in the absence of polyadenylation, whereas mos requires poly(A) extension to activate XFGFR translation. Our results demonstrate that mos and cdc2, in addition to functioning as key regulators of the meiotic cell cycle, cooperate in the translational activation of a specific maternal mRNA during oocyte maturation.
Culp
P A
PA
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California School of Medicine, San Francisco, California 94143-0556, USA.
Musci
T J
TJ
D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D008540:Meiosis; D008845:Microinjections; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009865:Oocytes; D010766:Phosphorylation; D011061:Poly A / Q000235:genetics; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D017231:Proto-Oncogene Proteins c-mos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014981:Xenopus
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D011061:Poly A; D011494:Protein Kinases; C102333:histone H1 kinase; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017231:Proto-Oncogene Proteins c-mos; D016203:CDC2 Protein Kinase; D020928:Mitogen-Activated Protein Kinases