Molecular cloning of vacuolar H(+)-pyrophosphatase and its expression during the development of pear fruit.
The cDNA of vacuolar proton-translocating inorganic pyrophosphatase was cloned from pear fruit. It contained an open reading frame of 2,301 nucleotides, coding for a polypeptide of 767 amino acids. The level of mRNA was very low in young fruit and increased with maturation, differing from the changes in the level of polypeptide.
Suzuki
Y
Y
Laboratory of Horticultural Science, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Maeshima
M
M
Yamaki
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005638:Fruit / Q000201:enzymology* / Q000235:genetics; D043564:Inorganic Pyrophosphatase; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000235:genetics*; D014617:Vacuoles / Q000201:enzymology*
D018076:DNA, Complementary; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase