title: Mode of action of novel 2-(benzylamino)-4-methyl-6-(trifluoro- methyl)-1,3,5-triazine herbicides: inhibition of photosynthetic electron transport and binding studies.
abstract: Novel 2-(benzylamino)-4-methyl-6-(trifluoromethyl)-1,3,5-triazines have the same 1,3,5-triazine skeleton as atrazine, although some of them, for example, 2-(3-chlorobenzylamino)-4-methyl-6-(trifluoromethyl)-1,3,5-tria zin e [pI(50)(spinach) = 7.21], show a >3 times stronger photosynthetic electron transport inhibitory activity than atrazine [pI(50)(spinach) = 6.72]. The new triazines have only one amino group at the triazine ring, and their molecular shapes are different from atrazine. The replacement of the bound [(14)C]atrazine by 1,3,5-triazines was tested to determine whether the novel 1,3,5-triazine analogues exhibit the same binding pattern at the D1-protein as atrazine. It was found that [(14)C]atrazine bound to the D1-protein was replaced by the triazine tested by a clearly competitive interaction. Obviously, the novel 1,3,5-triazines are attached to the same binding niche as atrazine.
authors.affiliation: Faculty of Agriculture, Tamagawa University, Machida-shi, Tokyo 194-8610, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ohki
authors.forename: N
authors.initials: N
authors.lastname: Kuboyama
authors.forename: K
authors.initials: K
authors.lastname: Koizumi
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Kohno
authors.forename: P
authors.initials: P
authors.lastname: Böger
authors.forename: K
authors.initials: K
authors.lastname: Wakabayashi
mesh_terms: D004579:Electron Transport / Q000187:drug effects; D006540:Herbicides / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects*; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D018724:Spinacia oleracea; D014227:Triazines
chemical_list: D006540:Herbicides; D010940:Plant Proteins; D014227:Triazines