title: The cytoplasmic domain of the platelet glycoprotein Ibalpha is phosphorylated at serine 609.
abstract: The alpha chain of the platelet von Willebrand factor receptor, glycoprotein (GP) Ib, is not known to be phosphorylated. Here, we report that the cytoplasmic domain of GPIbalpha is phosphorylated at Ser(609); this was detected by immunoblotting with an anti-phosphopeptide antibody, anti-pS609, that specifically recognizes the GPIbalpha C-terminal sequence S(606)GHSL(610) only when Ser(609) is phosphorylated. Immunoabsorption with anti-pS609 removed almost all of the GPIbalpha from platelet lysates, indicating a high proportion of GPIbalpha phosphorylation. Anti-pS609 inhibited GPIb-IX binding to the intracellular signaling molecule, 14-3-3zeta. Dephosphorylation of GPIb-IX with potato acid phosphatase inhibited anti-pS609 binding and also 14-3-3zeta binding. A synthetic phosphopeptide corresponding to the GPIbalpha C-terminal sequence (SIRYSGHpSL), but not a nonphosphorylated identical peptide, abolished GPIb-IX binding to 14-3-3zeta. Thus, phosphorylation at Ser(609) of GPIbalpha is important for 14-3-3zeta binding to GPIb-IX. In certain regions of spreading platelets, particularly at the periphery, there was a reduction in GPIbalpha staining by anti-pS609 as observed under a confocal microscope, indicating that a subpopulation of GPIbalpha molecules in these regions is dephosphorylated. These data suggest that phosphorylation and dephosphorylation at Ser(609) of GPIbalpha regulates GPIb-IX interaction with 14-3-3 and may play important roles in the process of platelet adhesion and spreading.
authors.affiliation: Department of Pharmacology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Bodnar
authors.forename: M
authors.initials: M
authors.lastname: Gu
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: G D
authors.initials: GD
authors.lastname: Englund
authors.forename: X
authors.initials: X
authors.lastname: Du
mesh_terms: D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion; D003593:Cytoplasm / Q000378:metabolism*; D010766:Phosphorylation; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D012694:Serine / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D010980:Platelet Membrane Glycoproteins; D011506:Proteins; D012694:Serine; D014446:Tyrosine 3-Monooxygenase