title: Lens-specific regulation of the thioredoxin-1 gene, but not thioredoxin-2, upon in vivo photochemical oxidative stress in the Emory mouse.
abstract: Thioredoxin (TRX)-1 and TRX-2 redox-regulatory genes were analyzed in the lens and some other tissues of the Emory mouse, a model for age-related human cataract. The cDNA transcripts of mRNAs encoding TRX-1 and TRX-2 genes were isolated and cloned by RT-PCR from the lens, liver, kidney, and tail, and the cDNA sequences were similar to the reported sequences of murine TRX-1 and TRX-2 genes. In vivo photochemical oxidative stress to the Emory mice resulted in fivefold upregulation of the lens TRX-1 gene at 3 weeks and declined thereafter. Western blot analysis revealed a fourfold increase of TRX-1 protein in the lens at 3 weeks after oxidative stress. The TRX-2 gene in the lens was not changed up to 5 weeks and decreased by 50% thereafter. However, the expressions of these genes in the liver, kidney, and tail were not changed. Fluorescent light or riboflavin alone did not affect the expressions of TRX-1 and TRX-2 genes in the lens. Thus, we show the expressions of TRX-1 and TRX-2 genes in the lens, liver, kidney, and tail and lens-specific upregulation of the TRX-1 gene and protein expressions, possibly as a protective response to the altered redox state of the lens after in vivo oxidative stress to the Emory mouse.
authors.affiliation: Membrane Biochemistry Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, New York, 10032, USA.
authors.forename: P G
authors.initials: PG
authors.lastname: Reddy
authors.forename: D K
authors.initials: DK
authors.lastname: Bhuyan
authors.forename: K C
authors.initials: KC
authors.lastname: Bhuyan
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002386:Cataract / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010777:Photochemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D013879:Thioredoxins