2,4-Nonadienal and benzaldehyde bioantimutagens in Fushimi sweet pepper (Fushimi-togarashi).
Fushimi sweet pepper, "Fushimi-togarashi", is one of the "Kyo-yasai", traditional vegetables, in Kyoto, Japan. The chloroform fraction of Fushimi sweet pepper showed bioantimutagenicity on UV induced mutation in Escherichia coli B/r WP2. The bioantimutagen was purified with silica gel chromatography and identified as 2, 4-nonadienal (ID(50) = 20 microg/plate) on the basis of GC retention time and EI-MS spectrum of authentic 2,4-nonadienal. The sweet pepper also contained a known bioantimutagen, benzaldehyde (ID(50) = 2 mg/plate). Additive bioantimutagenicity was also observed by 2, 4-nonadienal with benzaldehyde. 2,4-Nonadienal did not show bioantimutagenicity in an UV excision repair deficient strain, E. coli B/r WP2s uvrA(-)(). Furthermore no delay of the first cell division after UV irradiation was observed in E. coli B/r WP2. These results indicate that the bioantimutagenic activity of 2, 4-nonadienal on UV mutagenesis might depend on the excision repair system in E. coli B/r WP2.
Nakamura
Y
Y
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Japan.
Suganuma
E
E
Matsuo
T
T
Okamoto
S
S
Sato
K
K
Ohtsuki
K
K
D000447:Aldehydes / Q000302:isolation & purification / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001547:Benzaldehydes / Q000302:isolation & purification / Q000494:pharmacology*; D002212:Capsicum / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D008401:Gas Chromatography-Mass Spectrometry; D010946:Plants, Medicinal; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D000447:Aldehydes; D016587:Antimutagenic Agents; D001547:Benzaldehydes; C412886:2,4-nonadienal