Isolation and characterization of human DNA from mosquitoes (Culicidae).
Human DNA was prepared from mosquitoes (Culicidae) which were collected in a room shared by four human individuals. Several insects did not contain human blood and DNA preparation from them was not successful. However, high molecular weight human genomic DNA could be isolated from four insects. HLA-DQalpha and D1S80 analysis showed that the blood from one insect was a mixture from two persons, whereas the others contained blood from single individuals. Human DNA isolated 26 h after ingestion was still suitable for typing. These results showed that DNA isolated from mosquitoes is qualitatively and quantitatively sufficient for DNA typing and could be helpful to identify individuals involved in certain cases of body violence or captivity.
Kreike
J
J
Institut für Gerichtliche Medizin, Universität Wien, Sensengasse 2, A-1090 Wien, Austria.
Kampfer
S
S
D000483:Alleles; D000818:Animals; D001801:Blood Stains; D009033:Culicidae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005787:Gene Frequency; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction
D006683:HLA-DQ Antigens; D004247:DNA