The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast.
The chloroplastic outer envelope protein OEP24 from pea forms a high-conductance low specificity solute channel as shown by in vitro studies. In order to establish its function also in an in vivo-like system, the gene encoding OEP24 was transformed into a yeast strain which lacks the general mitochondria solute channel porin, also known as voltage-dependent anion channel (VDAC). Transformation of the yeast VDAC(-) strain with the OEP24 gene resulted in the recovery of a phenotype indistinguishable from the wild-type. The OEP24 polypeptide is targeted to the mitochondrial outer membrane in this heterologous system. We conclude that OEP24 forms a solute channel in pea chloroplasts in planta.
Röhl
T
T
Botanisches Institut, Universität Kiel, Am Botanischen Garten 1-9, D-24118, Kiel, Germany.
Motzkus
M
M
Soll
J
J
D002736:Chloroplasts / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D007425:Intracellular Membranes / Q000502:physiology; D007473:Ion Channels / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008928:Mitochondria / Q000502:physiology*; D018532:Pisum sativum / Q000502:physiology*; D010539:Permeability; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018272:Porins / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D050994:Voltage-Dependent Anion Channels
D005656:Fungal Proteins; D007473:Ion Channels; C113819:OEP24 protein, Pisum sativum; D010940:Plant Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels