title: Transglycosylation of naringin by Bacillus stearothermophilusMaltogenic amylase to give glycosylated naringin.
abstract: Naringin, a bitter compound in citrus fruits, was transglycosylated by Bacillus stearothermophilus maltogenic amylase reaction with maltotriose to give a series of mono-, di-, and triglycosylnaringins. Glycosylation products of naringin were observed by TLC and HPLC. The major glycosylation product was purified by using a Sephadex LH-20 column. The sturcture was determined by using MALDI-TOF MS, methylation analysis, and (1)H and (13)C NMR. The major transglycosylation product was maltosylnaringin, in which the maltose unit was attached by an alpha-1-->6 glycosidic linkage to the D-glucose moiety of naringin. This product was 250 times more soluble in water and 10 times less bitter than naringin.
authors.affiliation: Department of Food Science and Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University, Suwon 441-744, Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: J C
authors.initials: JC
authors.lastname: Kim
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Kitaoka
authors.forename: C S
authors.initials: CS
authors.lastname: Park
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: M J
authors.initials: MJ
authors.lastname: Ra
authors.forename: T W
authors.initials: TW
authors.lastname: Moon
authors.forename: J F
authors.initials: JF
authors.lastname: Robyt
authors.forename: K H
authors.initials: KH
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002957:Citrus; D044950:Flavanones; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006031:Glycosylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; C120909:glucan 1,4-alpha-maltohydrolase; C005274:naringin