title: Some sulfur-containing metabolites of tri-n-butyltin chloride in male rats.
abstract: In an attempt to elucidate metabolic destination of TBTO, sulfur-containing metabolites were investigated in the urine. Tri-n-butyltin chloride (TBTC), tri-n-butyltin oxide (TBTO), and their in vitro metabolites in rat liver microsomal enzyme systems, di-n-butyl(3-hydroxybutyl)tin chloride (T3OH), di-n-butyl(3-oxobutyl)tin chloride (T3CO), dibutyltin dichloride (DBTC), and monobutyltin trichloride (MBTC), were intraperitoneally administered to rats. In particular, administration of T3OH and T3CO gave higher amounts of mercapturic acid derivatives, such as N-acetyl-S-(3-oxobutyl)-L-cysteine (3CO-MA) and N-acetyl-S-(3-hydroxybutyl)-L-cysteine (3OH-MA), than TBTC or TBTO. On the other hand, DBTC and MBTC did not yield measurable amounts of 3CO-MA and/or 3OH-MA. The appearance of organotin metabolites in urine indicates that T3OH, T3CO, and hypothesized secondary metabolites, such as n-butyl(3-hydroxybutyl)(3-oxobutyl)tin chloride, n-butyl(3-hydroxybutyl)(4-hydroxybutyl)tin chloride, etc., are subject to the action of glutathione S-transferase to give mercapturic acid derivatives. These sulfur-containing metabolites (3CO-MA and 3OH-MA) were also found in control rat urine.
authors.affiliation: Division of Foods, National Institute of Health Sciences, 18-1 Kamiyoga 1-chome, Setagaya-ku, Tokyo 158-8501, Japan. tsuzuki@nihs.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: M
authors.initials: M
authors.lastname: Uchiyama
authors.forename: M
authors.initials: M
authors.lastname: Murayama
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008954:Models, Biological; D051381:Rats; D013455:Sulfur / Q000652:urine*; D014220:Trialkyltin Compounds / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: D014220:Trialkyltin Compounds; C005961:bis(tri-n-butyltin)oxide; D013455:Sulfur