Exploration of the association between apoptosis and cleft palate.
OBJECTIVE
To explore the significance and effect of apoptosis in the development of cleft palate induced by dexamethasone (DEX).


METHODS
Various doses of DEX (10-50 mg/kg) were injected intraperitoneally into Kunming mice on gestation day (GD) 12. The cranio-maxillary sections of samples were labeled by HRP-avidin-biotin-dUTP in situ end-labeling (ISEL) and were observed morphologically and quantitatively under a light microscope and electron microscope.


RESULTS
ISEL positive cells were found in treatment groups. The means of sums and cleft palate incidence have a positive correlation with the doses of DEX (r = 0.9911/0.9978).


CONCLUSIONS
A relationship exists between an increased level of apoptosis and the development of cleft palate induced by DEX.
Chen
Y
Y
Department of Oral and Maxillofacial Surgery, College of Stomatology, Sun Yat-sen University of Medical Sciences (SUMS), Guangzhou, P. R. China.
Huang
H
H
D000818:Animals; D017209:Apoptosis; D002972:Cleft Palate / Q000139:chemically induced / Q000196:embryology* / Q000473:pathology; D003907:Dexamethasone; D005260:Female; D020287:In Situ Nick-End Labeling; D051379:Mice
D003907:Dexamethasone