title: ATP- and ADP-dnaA protein, a molecular switch in gene regulation.
abstract: DnaA protein functions by binding to asymmetric 9mer DNA sites, the DnaA boxes. ATP-DnaA and ADP-DnaA bind to 9mer DnaA boxes with equal affinity, but only ATP-DnaA protein binds in addition to an as yet unknown 6mer site, the ATP-DnaA box AGATCT, or a close match to it. ATP-DnaA protein binding to ATP-DnaA boxes is restricted to sites located in close proximity to DnaA boxes, suggesting that protein-protein interaction is required for its stabilization. We show that ATP-DnaA represses dnaA transcription much more efficiently than ADP-DnaA. DnaA is thus a regulatory molecule that, depending on the adenosine nucleotide bound, can bind to different sequences and thereby fulfill distinct functions.
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin-Dahlem, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Speck
authors.forename: C
authors.initials: C
authors.lastname: Weigel
authors.forename: W
authors.initials: W
authors.lastname: Messer
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002453:Cell Cycle / Q000235:genetics; D004247:DNA / Q000378:metabolism*; D018983:DNA Footprinting; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D009838:Oligodeoxyribonucleotides; D012097:Repressor Proteins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004247:DNA