title: Preparation of HeLa cell nuclear and cytosolic S100 extracts for in vitro splicing.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida, USA.
authors.forename: A
authors.initials: A
authors.lastname: Mayeda
authors.forename: A R
authors.initials: AR
authors.lastname: Krainer
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002457:Cell Extracts / Q000302:isolation & purification*; D002467:Cell Nucleus / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000302:isolation & purification; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics*
chemical_list: D002457:Cell Extracts; D009687:Nuclear Proteins; D012322:RNA Precursors