Conservation of the pyrrolnitrin biosynthetic gene cluster among six pyrrolnitrin-producing strains.
The prnABCD gene cluster from Pseudomonas fluorescens encodes the biosynthetic pathway for pyrrolnitrin, a secondary metabolite derived from tryptophan which has strong anti-fungal activity. We used the prn genes from P. fluorescens strain BL915 as a probe to clone and sequence homologous genes from three other Pseudomonas strains, Burkholderia cepacia and Myxococcus fulvus. With the exception of the prnA gene from M. fulvus59% similar among the strains, indicating that the biochemical pathway for pyrrolnitrin biosynthesis is highly conserved. The prnA gene from M. fulvus is about 45% similar to prnA from the other strains and contains regions which are highly conserved among all six strains.
Hammer
P E
PE
Novartis Agribusiness Biotechnology Research, P.O. Box 12257, Research Triangle Park, NC 27709, USA.
Burd
W
W
Hill
D S
DS
Ligon
J M
JM
van Pée
K
K
D000935:Antifungal Agents / Q000096:biosynthesis; D001483:Base Sequence; D015139:Blotting, Southern; D016956:Burkholderia cepacia / Q000235:genetics; D017124:Conserved Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D016940:Myxococcus / Q000235:genetics; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism; D011764:Pyrrolnitrin / Q000096:biosynthesis*; D016415:Sequence Alignment
D000935:Antifungal Agents; D011764:Pyrrolnitrin