title: Overexpression of laminin gamma2 chain monomer in invading gastric carcinoma cells.
abstract: Laminin (LN)-5, a heterotrimer of alpha3, beta3, and gamma2 chains, has been suggested to be involved in tumor cell invasion. The present immunohistochemical study investigated the distribution of the LN gamma2 chain in 48 different human gastric adenocarcinomas. The immunohistochemical analysis showed two distinct patterns of LN gamma2 chain expression: (a) extracellular deposition; and (b) cytoplasmic accumulation. The extracellular deposition of the LN gamma2 chain was typically observed at neoplastic basement membranes of well-differentiated adenocarcinomas. The immunoreactivity was continuous along tumor basement membranes in these tumors but was irregular and diffuse in poorly differentiated carcinomas. These tumor cells coexpressed the LN alpha3 and beta3 chains, suggesting that the LN gamma2 chain was deposited as the LN-5 complex. In contrast, tumor cells at the invading fronts showed strong cytoplasmic staining for the LN gamma2 chain without any detectable signal for the LN alpha3 or beta3 chain in both well- and poorly differentiated carcinomas. On the other hand, in vitro analysis by two-dimensional SDS-PAGE demonstrated that human gastric carcinoma cells secrete a high level of LN gamma2 chain monomer in addition to the LN-5 complex into culture medium. These results indicate that the LN gamma2 chain can be secreted as a single subunit and might be involved in tumor cell invasion.
authors.affiliation: Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Koshikawa
authors.forename: K
authors.initials: K
authors.lastname: Moriyama
authors.forename: H
authors.initials: H
authors.lastname: Takamura
authors.forename: H
authors.initials: H
authors.lastname: Mizushima
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: S
authors.initials: S
authors.lastname: Yanoma
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D000097794:Kalinin
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007797:Laminin; D000097794:Kalinin; C400567:LAMC2 protein, human; C095567:laminin alpha 3