Gene transfer in the gastrointestinal tract.
The maximum in vivo transfer rate of plasmid pAMbeta1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not detected in liquid culture matings. Our findings demonstrate that in vitro methods, such as forced filter mating and liquid mating, underestimate the in vivo rates of gene transfer.
Netherwood
T
T
Department of Biological and Nutritional Sciences, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE1 7RU, Great Britain. trudy.netherwood@newcastle.ac.uk
Bowden
R
R
Harrison
P
P
O'Donnell
A G
AG
Parker
D S
DS
Gilbert
H J
HJ
D000818:Animals; D001406:Bacillaceae / Q000235:genetics; D002480:Cellulase / Q000235:genetics; D002645:Chickens; D003227:Conjugation, Genetic; D004064:Digestive System / Q000187:drug effects / Q000382:microbiology*; D016984:Enterococcus faecium / Q000235:genetics*; D018014:Gene Transfer Techniques; D010957:Plasmids; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology
D002480:Cellulase