title: Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells is predictive of vascular infection.
abstract: Abdominal aortic aneurysm tissue and peripheral blood mononuclear cells (PBMC) of 41 consecutive subjects undergoing abdominal aortic aneurysm surgery were analyzed by polymerase chain reaction (PCR) for the presence of Chlamydia pneumoniae, Mycoplasma pneumoniae, and Helicobacter pylori DNA. Twenty patients (49%) were positive for C. pneumoniae DNA-16 (39%) in both PBMC and aneurysm tissue, 3 (7.3%) in PBMC only, and 1 (2.4%) in the artery specimen only. Previous exposure to C. pneumoniae was confirmed in 19 (95%) of the 20 PCR positive subjects by C. pneumoniae-specific serology, using the microimmunofluorescence test. None was positive for H. pylori or M. pneumoniae DNA, either in the PBMC or in the artery specimens. In conclusion, carriage of C. pneumoniae DNA is common both in PBMC and in abdominal aortic tissue from patients undergoing abdominal aneurysm surgery. Blood PCR may be a useful tool for identifying subjects carrying C. pneumoniae in the vascular wall.
authors.affiliation: Istituto di Tisiologia e Malattie dell'Apparato Respiratorio, Università degli Studi di Milano, Pad. Litta, IRCCS Ospedale Maggiore di Milano. via F. Sforza, 35 I-20122 Milano, Italy. francesco.blasi@unimi.it
authors.forename: F
authors.initials: F
authors.lastname: Blasi
authors.forename: J
authors.initials: J
authors.lastname: Boman
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: G
authors.initials: G
authors.lastname: Melissano
authors.forename: R
authors.initials: R
authors.lastname: Chiesa
authors.forename: R
authors.initials: R
authors.lastname: Cosentini
authors.forename: P
authors.initials: P
authors.lastname: Tarsia
authors.forename: Y
authors.initials: Y
authors.lastname: Tshomba
authors.forename: M
authors.initials: M
authors.lastname: Betti
authors.forename: M
authors.initials: M
authors.lastname: Alessi
authors.forename: N
authors.initials: N
authors.lastname: Morelli
authors.forename: L
authors.initials: L
authors.lastname: Allegra
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001012:Aorta, Abdominal / Q000382:microbiology*; D017544:Aortic Aneurysm, Abdominal / Q000382:microbiology* / Q000601:surgery; D001018:Aortic Diseases / Q000175:diagnosis* / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests
chemical_list: D004269:DNA, Bacterial