title: Tdr2, a new zebrafish transposon of the Tc1 family.
abstract: We describe here Tdr2, a new class of Tc1-like transposons in zebrafish. Tdr2 was identified from the genomic sequence of a zebrafish PAC (P1 artificial chromosome) clone, and fragments of Tdr2 were found in several zebrafish EST (expressed sequence tag) sequences. Predicted translation of the Tdr2 transposase gene showed that it was most closely related to Caenorhabditis elegans Tc3A, suggesting an ancient origin of the Tdr2 transposon. Tdr2 spans 1. 1kb and is flanked by inverted repeats of approx. 100bp. The 5' repeat is itself composed of an inverted repeat, raising the possibility of the formation of a cruciform DNA structure. Tdr2 transposons may facilitate the development of novel transposon-based tools for the genetic analysis of zebrafish.
authors.affiliation: Department of Haematology, Cambridge University, MRC Centre, Hills Road, Cambridge, UK. bg200@cam.ac.uk
authors.forename: B
authors.initials: B
authors.lastname: Göttgens
authors.forename: L M
authors.initials: LM
authors.lastname: Barton
authors.forename: D
authors.initials: D
authors.lastname: Grafham
authors.forename: M
authors.initials: M
authors.lastname: Vaudin
authors.forename: A R
authors.initials: AR
authors.lastname: Green
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D019895:Transposases / Q000235:genetics; D015027:Zebrafish / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004247:DNA; D019895:Transposases