The BM2 protein of influenza B virus is synthesized in the late phase of infection and incorporated into virions as a subviral component.
The influenza B virus genome RNA segment 7 encodes the M1 and BM2 proteins. The BM2 protein is synthesized by a coupled translational termination-reinitiation mechanism at the overlapping stop-start pentanucleotide in a bicistronic mRNA transcribed from RNA segment 7. However, features and functions of this protein remain unclear. In this study the BM2 protein was characterized by using an antiserum raised to the BM2 protein of influenza virus strain B/Yamagata/1/73. In cells infected with B/Yamagata virus the alphaBM2 antibody specifically detected the BM2 protein with a molecular mass of 12 kDa and also a polypeptide with a molecular mass of 17 kDa. When infected cells were labelled with 32Pi and immunoprecipitated with the alphaBM2 antibody, the 32P-labelled 17 kDa polypeptide was specifically precipitated. In the presence of casein kinase inhibitor CKI-7 the synthesis of the 17 kDa and BM2 proteins was completely suppressed, although other viral proteins, except for the polymerase protein, were synthesized normally. These results suggest that the 17 kDa species is a phosphorylated form of the BM2 protein. These species were substantially synthesized in the late phase of infection and localized in the cytoplasm throughout infection. Moreover, they were transported to the plasma membrane and thereafter were incorporated into virions. These results therefore suggest that the BM2 and the 17 kDa proteins are necessary for the life-cycle of influenza B virus.
Odagiri
Takato
T
Department of Microbiology, Kanazawa Medical University, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan1.
Hong
Jin
J
Department of Microbiology, Kanazawa Medical University, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan1.
Ohara
Yoshiro
Y
Department of Microbiology, Kanazawa Medical University, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan1.
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D004279:DNA, Viral; D004285:Dogs; D006801:Humans; D009981:Influenza B virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D014771:Virion / Q000378:metabolism; D019065:Virus Assembly
C064964:BM2 polypeptide, influenza B virus; D004279:DNA, Viral; D010455:Peptides; D014764:Viral Proteins