The zymogen prothrombin stimulates cell locomotion and calcium influx in murine osteosarcoma cells by different mechanism from thrombin.
The coagulation system plays a role in tumor invasion and metastasis. Prothrombin was previously found to stimulate the motility of melanoma cells. We compared the effect of prothrombin and thrombin on cell motility, calcium influx and actin stress filament contraction in highly metastatic osteosarcoma LM8 cells. Prothrombin and thrombin stimulated chemotaxis and chemokinesis of LM8 cells in a dose-dependent manner. Calcium influx was significantly increased after stimulation with thrombin and prothrombin. Thrombin and prothrombin markedly stimulated actin contraction in LM8 cells. Hirudin inhibited the effects of thrombin but not those of prothrombin. Prothrombin appears to stimulate cell locomotion, actin contraction, calcium influx in LM8 cells by different mechanism from thrombin.
Sano
T
T
Department of Molecular Pathobiology, Mie University School of Medicine, Tsu-city, Mie, Japan.
Gabazza
E C
EC
Zhou
H
H
Takeya
H
H
Hayashi
T
T
Ido
M
M
Adachi
Y
Y
Uchida
A
A
Suzuki
K
K
D000199:Actins / Q000502:physiology; D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects*; D002633:Chemotaxis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000494:pharmacology*; D006801:Humans; D008856:Microscopy, Fluorescence; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D010446:Peptide Fragments / Q000494:pharmacology; D011498:Protein Precursors / Q000494:pharmacology; D011516:Prothrombin / Q000378:metabolism / Q000494:pharmacology*; D011869:Radioligand Assay; D013917:Thrombin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000199:Actins; D004792:Enzyme Precursors; D010446:Peptide Fragments; D011498:Protein Precursors; C029733:prothrombin fragment 1; D011516:Prothrombin; D013917:Thrombin; D002118:Calcium