Organization and chromosomal localization of the human endothelial protein C receptor gene.
Endothelial protein C receptor (EPCR), present on endothelial cells of relatively large veins and arteries, plays a role in the enhancement of protein C activation by the thrombin-thrombomodulin complex. In the present study, we determined the organization and the complete nucleotide sequence of the human EPCR gene using polymerase chain reaction-direct sequencing method. The transcription initiation site of the EPCR gene was also determined by the cap site hunting method, using a cap site cDNA prepared from human placenta. The human EPCR gene spanned approx. 6 kb and was composed of four exons and three introns. All exon-intron boundaries agreed with the GT-AG rule. The 5'-flanking region (300 bp) of the EPCR gene contained a putative AP1-binding site, two Sp1-binding sites and two AP2-binding sites, but not definite TATAA or CCAAT sequences. Fluorescence in situ hybridization analysis showed that the EPCR gene is located in chromosome 20q11.2.
Hayashi
T
T
Department of Molecular Pathobiology, Mie University School of Medicine, Tsu-city, Japan.
Nakamura
H
H
Okada
A
A
Takebayashi
S
S
Wakita
T
T
Yuasa
H
H
Okumura
K
K
Suzuki
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001779:Blood Coagulation Factors; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20; D004247:DNA; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D018180:Thrombomodulin / Q000235:genetics
D001779:Blood Coagulation Factors; D011956:Receptors, Cell Surface; D018180:Thrombomodulin; C091963:activated protein C receptor; D004247:DNA