SCOP, a novel gene product expressed in a circadian manner in rat suprachiasmatic nucleus.
To elucidate the mechanism of the circadian rhythm, genes differentially expressed during subjective day and night in the rat suprachiasmatic nucleus (SCN), a circadian oscillator in mammals, were surveyed by a differential display method. We isolated a novel gene, scop (SCN circadian oscillatory protein), that was expressed in a circadian manner in the SCN. SCOP protein is predominantly expressed in the brain and has domains including a pleckstrin homology domain, leucine-rich repeats, a protein phosphatase 2C-like domain and a glutamine-rich region. The structural feature of SCOP protein suggests its role in the intracellular signaling in the SCN.
Shimizu
K
K
Division of Protein Metabolism, Institute for Protein Research, Osaka University, Suita, Japan. ashimkim@mail.ecc.u-tokyo.ac.jp
Okada
M
M
Takano
A
A
Nagai
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D010749:Phosphoprotein Phosphatases; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C400601:PHLPP1 protein, human; C553451:PHLPP1 protein, rat; D010749:Phosphoprotein Phosphatases