Laser desorption of DNA oligomers larger than one kilobase from cooled 4-nitrophenol.
A unique matrix system consisting mostly of 4-nitrophenol has shown to be very effective for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of large DNA oligomers when a cooled sample stage was used to prevent the sublimation of this matrix under vacuum. Using this 4-nitrophenol matrix with UV laser desorption, detection of picomole quantities of DNA oligomers containing up to approximately 800 nucleotides was routinely achieved. The effectiveness of this matrix was further demonstrated by the observation of a double-stranded DNA oligomer larger than 1000 base pairs, seen as a denatured single-stranded species, with a molecular ion mass exceeding 300 000 Da. The potential applications of 4-nitrophenol as a matrix for DNA sizing are discussed.
Lin
H
H
GeneTrace Systems Inc., 1401 Harbor Bay Parkway, Alameda, CA 94502, USA.
Hunter
J M
JM
Becker
C H
CH
D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D007202:Indicators and Reagents; D008970:Molecular Weight; D009596:Nitrophenols; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009596:Nitrophenols; D009838:Oligodeoxyribonucleotides; D004247:DNA; C024836:4-nitrophenol