title: 4-Hydroxybenzoate uptake in an isolated soil Acinetobacter sp.
abstract: The isolated soil bacteria Acinetobacter strain BEM2 is able to utilize some xenobiotic aromatic compounds as a carbon source. In this study the metabolism of 4-hydroxybenzoate (4-HBA) by strain BEM2 was characterized. Degradation involved a meta-cleavage pathway yielding 3,4-dihydroxybenzoate (3,4-DHBA) as an intermediate and CO(2) as the principal product from the C atoms in the aromatic ring. 4-HBA uptake was studied, and the kinetic parameters were determined. The uptake was shown to be directly coupled to ATP hydrolysis and its synthesis, according to the Mitchell chemiosmotic hypothesis.
authors.affiliation: Departamento de Física Aplicada, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Allende
authors.forename: A
authors.initials: A
authors.lastname: Gibello
authors.forename: A
authors.initials: A
authors.lastname: Fortún
authors.forename: G
authors.initials: G
authors.lastname: Mengs
authors.forename: E
authors.initials: E
authors.lastname: Ferrer
authors.forename: M
authors.initials: M
authors.lastname: Martín
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000332:Aerobiosis; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D001693:Biological Transport, Active; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D010226:Parabens / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D004791:Enzyme Inhibitors; D010226:Parabens; D000255:Adenosine Triphosphate; C038193:4-hydroxybenzoic acid