ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex.
The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit phosphatidylinositol-4-OH kinase-beta and a still unidentified phosphatidylinositol-4-phosphate-5-OH kinase to the Golgi complex, resulting in a potent stimulation of synthesis of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate; this ability is independent of its activities on coat proteins and PLD. Phosphatidylinositol-4-OH kinase-beta is required for the structural integrity of the Golgi complex: transfection of a dominant-negative mutant of the kinase markedly alters the organization of the organelle.
Godi
A
A
Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Santa Maria Imbaro, Italy.
Pertile
P
P
Meyers
R
R
Marra
P
P
Di Tullio
G
G
Iurisci
C
C
Luini
A
A
Corda
D
D
De Matteis
M A
MA
D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism*; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008563:Membrane Lipids / Q000096:biosynthesis*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000096:biosynthesis*; D010739:Phospholipase D / Q000378:metabolism; D051381:Rats
D008563:Membrane Lipids; D019269:Phosphatidylinositol 4,5-Diphosphate; D019870:1-Phosphatidylinositol 4-Kinase; D010739:Phospholipase D; D020727:ADP-Ribosylation Factors