Determination of the wax ester content in olive oils. Improvement in the method proposed by EEC regulation 183/93.
A simpler and faster procedure than the official one described in document IV of European Economic Community Regulation 183/93 is proposed. The wax ester fraction is isolated from triglycerides using a commercially available silica gel column and carbon tetrachloride as eluent. The recovered wax ester fraction, with the addition of a suitable internal standard solution, is analyzed by gas chromatography. A column with a 65% phenyl methyl silicone stationary phase allows a satisfying separation of wax ester fraction in comparison with both a steryl ester and a light fraction eluted before the internal standard. Furthermore, also the single components of the wax ester fraction are suitably separated.
Nota
G
G
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici, Napoli, Italy.
Naviglio
D
D
Romano
R
R
Sabia
V
V
Musso
S S
SS
Improta
C
C
D002849:Chromatography, Gas; D004952:Esters; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*
D004952:Esters; D000069463:Olive Oil; D010938:Plant Oils