Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the Dazl orthologue Boule.
Boule, a Drosophila orthologue of the vertebrate Dazl fertility factors, is a testis-specific regulator of meiotic entry and germline differentiation. Mutations inactivating either Boule, which is an RNA-binding protein, or Twine, which is a Cdc25-type phosphatase, block meiotic entry in males. Here we show that twine and boule interact genetically. We also find that protein expression from twine messenger RNA correlates with cytoplasmic accumulation of Boule and is markedly reduced by boule mutations. Remarkably, heterologous expression of Twine rescues the boule meiotic-entry defect, indicating that the essential function of Boule at the transition from G2 to M phase during meiosis is in the control of Twine translation.
Maines
J Z
JZ
Center for Molecular Genetics, University of California at San Diego, La Jolla 92093-0634, USA.
Wasserman
S A
SA
D000818:Animals; D002453:Cell Cycle; D018385:Centrosome / Q000502:physiology / Q000648:ultrastructure; D000075364:Deleted in Azoospermia 1 Protein; D004330:Drosophila / Q000502:physiology; D029721:Drosophila Proteins; D019476:Insect Proteins / Q000378:metabolism*; D008297:Male; D008540:Meiosis; D009845:Oligospermia; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology*; D013737:Testis / Q000502:physiology; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
C095668:DAZ1 protein, human; C101358:DAZL protein, human; D000075364:Deleted in Azoospermia 1 Protein; D029721:Drosophila Proteins; D019476:Insect Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; C099859:bol protein, Drosophila; C075186:twe protein, Drosophila; D017027:Protein Tyrosine Phosphatases; D001616:beta-Galactosidase