Does storage affect epifluorescence microscopic counts of total bacteria in freshwater samples?
Preservation with formalin and storage at 4 degrees C of freshwater samples, associated with sonication before counting, proved to be efficient for a few days of storage (no differences with the initial cell count within 7 d of storage); after 8 months, 95% of the initial count was preserved. This procedure always gave higher counts than those obtained from the freezing technique of filter storage at -18 degrees C after sample filtration.
Garabétian
F
F
Centre d'écologie des systèmes aquatiques continentaux, UMR CNRS C5576-université Paul-Sabatier, Toulouse, France. garabet@mail.cict.fr
Petit
M
M
Lavandier
P
P
D003080:Cold Temperature; D004356:Drug Storage; D005374:Filtration; D005404:Fixatives / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005615:Freezing; D005618:Fresh Water / Q000382:microbiology*; D008856:Microscopy, Fluorescence; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D014871:Water Microbiology
D005404:Fixatives; D005557:Formaldehyde