title: Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus.
abstract: Studies were conducted to determine effects of intrauterine administration of recombinant ovine interferon tau (IFNtau), placental lactogen (PL), and growth hormone (GH) on endometrial function. In the first study, administration of IFNtau to cyclic ewes for one period (Days 11-15) resulted in an interestrous interval (IEI) of approximately 30 days, whereas administration for two periods (Days 11-15 and Days 21-25) extended the IEI to greater than 50 days. Administration of IFNtau from Days 11 to 15 and of PL or GH from Days 21 to 25 failed to extend the IEI more than for IFNtau alone. In the second study, effects of IFNtau, PL, and GH on endometrial differentiation and function were determined in ovariectomized ewes receiving ovarian steroid replacement therapy. Endometrial expression of mRNAs for estrogen receptor (ER), progesterone receptor (PR), and oxytocin receptor (OTR) were not affected by PL or GH treatment; however, uterine milk protein mRNA levels and stratum spongiosum gland density were increased by both PL and GH treatments. Collectively, results indicated that 1) PL and GH do not regulate endometrial PR, ER, and OTR expression or affect corpus luteum life span; 2) down-regulation of epithelial PR expression is requisite for progesterone induction of secretory gene expression in uterine glandular epithelium; 3) effects of PL and GH on endometrial function require IFNtau; and 4) PL and GH regulate endometrial gland proliferation and perhaps differentiated function.
authors.affiliation: Center for Animal Biotechnology and Genomics, Texas A&M University System Health Science Center, and Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
authors.forename: T E
authors.initials: TE
authors.lastname: Spencer
authors.forename: A
authors.initials: A
authors.lastname: Gray
authors.forename: G A
authors.initials: GA
authors.lastname: Johnson
authors.forename: K M
authors.initials: KM
authors.lastname: Taylor
authors.forename: A
authors.initials: A
authors.lastname: Gertler
authors.forename: E
authors.initials: E
authors.lastname: Gootwine
authors.forename: T L
authors.initials: TL
authors.lastname: Ott
authors.forename: F W
authors.initials: FW
authors.lastname: Bazer
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000502:physiology; D004717:Endometrium / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics; D013006:Growth Hormone / Q000494:pharmacology*; D007370:Interferon Type I / Q000494:pharmacology*; D019394:Ki-67 Antigen / Q000032:analysis; D010052:Ovariectomy; D010928:Placental Lactogen / Q000494:pharmacology*; D011257:Pregnancy Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011960:Receptors, Estrogen / Q000235:genetics; D018045:Receptors, Oxytocin / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D015843:Serpins; D012756:Sheep
chemical_list: D006023:Glycoproteins; D007370:Interferon Type I; D019394:Ki-67 Antigen; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D018045:Receptors, Oxytocin; D011980:Receptors, Progesterone; D011994:Recombinant Proteins; D015843:Serpins; C020947:interferon tau; C029708:uterine luminal fluid proteins; D013006:Growth Hormone; D010928:Placental Lactogen