Absence of cecal secondary bile acids in gnotobiotic mice associated with two human intestinal bacteria with the ability to dehydroxylate bile acids in vitro.
Germ-free mice were orally inoculated with human intestinal 7alpha-dehydroxylating bacterial strains to evaluate their ability to transform bile acids in vivo. Three weeks after inoculation of the bacteria, cecal bile acids were examined. Among free-form bile acids, only beta-muricholic acid was detected in the cecal contents of gnotobiotic mice associated with Bacteroides distasonis strain K-5. No secondary bile acid was observed in the cecal contents of any of the gnotobiotic mice associated with 7alpha-dehydroxylating bacteria, Clostridium species strain TO-931 or Eubacterium species strain 36S.
Narushima
S
S
Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Science, The University of Tokyo, Japan.
Itoh
K
K
Takamine
F
F
Uchida
K
K
D000818:Animals; D001439:Bacteroides / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000737:chemistry / Q000378:metabolism*; D002432:Cecum / Q000382:microbiology*; D002793:Cholic Acids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003013:Clostridium / Q000378:metabolism*; D005051:Eubacterium / Q000378:metabolism*; D005260:Female; D005856:Germ-Free Life; D006801:Humans; D006900:Hydroxylation; D051379:Mice; D008807:Mice, Inbred BALB C
D001647:Bile Acids and Salts; D002793:Cholic Acids; C004821:muricholic acid