Influence of inorganic phosphate and energy state on force in skinned cardiac muscle from freshwater turtle and rainbow trout.
Inorganic phosphate, which increases in the hypoxic cardiac cell, depresses force development. The cardiac muscle of freshwater turtle maintains a remarkably high contractility during hypoxia; this may involve a low sensitivity to phosphate. Therefore, freshwater turtle and rainbow trout were compared with regard to Ca(2+)-activated force in skinned atrial trabeculae in a bath containing 3 mM ATP buffered by 15 mM creatine phosphate in the presence of creatine kinase. For turtle, an increase in phosphate from 0 mM to either 6 mM or 12 mM reduced maximal force by 50% and 80% respectively, whereas the Ca2+ activity eliciting half maximal force (Ca0.5) was increased by 70% in 6 mM and could not be reliably recorded in 12 mM. For trout, the effects of phosphate were less pronounced. An increase from 0 mM to 12 mM did not affect maximal force significantly, but elevated Ca0.5 by 70%. Hypoxia increases ADP as creatine phosphate is shifted to creatine, therefore, creatine phosphate was changed from 15 mM to 3 mM and creatine from 0 mM to 12 mM. After these changes, the elevation of phosphate from 0 mM to 12 mM had no significant effects for either turtle or trout. In conclusion, the high performance of turtle cardiac muscle during hypoxia does not involve a low sensitivity of the contractile system to phosphate. In addition, the effect of increased phosphate seems to be offset by a concomitant increase in ADP.
Jensen
M A
MA
Department of Zoophysiology, University of Aarhus, Denmark.
Gesser
H
H
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D003401:Creatine / Q000378:metabolism; D004734:Energy Metabolism; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D010725:Phosphocreatine / Q000378:metabolism; D013045:Species Specificity; D014426:Turtles / Q000502:physiology*
D010710:Phosphates; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D003401:Creatine