title: Detection of porcine circovirus from lesions of a pig with wasting disease in Japan.
abstract: A wasting disease characterized by progressive weight loss and dyspnea has been observed in weaning pigs on a farm in Yamagata Prefecture in 1998. Histopathologic findings in an affected pig were bronchointerstitial pneumonia and intracytoplasmic clusters of basophilic inclusions in macrophages of lymph nodes, which were similar to those in pigs with postweaning multisystemic wasting syndrome (PMWS) recently reported in North America and Europe. Porcine circovirus (PCV)-like particles were observed in bronchial lymph node of the pig by electron microscopy, and PCV antigens were detected in the lesions by immunohistochemical staining. PCV DNA was also detected in the lung and tonsil by PCR, and restriction fragment length polymorphism analysis of the PCR products with HinfI showed the same type of the PCV associated with PMWS (pmws PCV). Homology of nucleotide sequences between the PCR product and corresponding regions of published pmws PCV genomes was very high. These results indicated that virus detected in this study was pmws PCV. To our knowledge, this is the first report on the presence of pmws PCV in Japan.
authors.affiliation: Yamagata Central Livestock Hygiene Service Center, Yamagata, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Onuki
authors.forename: K
authors.initials: K
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Togashi
authors.forename: K
authors.initials: K
authors.lastname: Kawashima
authors.forename: A
authors.initials: A
authors.lastname: Taneichi
authors.forename: H
authors.initials: H
authors.lastname: Tsunemitsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018173:Circoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D007150:Immunohistochemistry; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D008198:Lymph Nodes / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000662:veterinary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000821:virology*; D019282:Wasting Syndrome / Q000473:pathology / Q000662:veterinary* / Q000821:virology
chemical_list: D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases