The URNA genes of herpesvirus saimiri (strain C488) are dispensable for transformation of human T cells in vitro.
The herpesvirus saimiri strain C488 genome contains five genes for small nuclear RNAs, termed herpesvirus saimiri URNAs (or HSURs). Using a cosmid-based approach, all HSURs were precisely deleted from the genome. The mutant virus replicated at levels that were similar to those of wild-type viruses in OMK cells. Although the HSURs are expressed in wild-type virus-transformed human T-cell lines, the deletion does not affect viral transformation in cell culture.
Ensser
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Institut für Klinische und Molekulare Virologie der Universität Erlangen-Nürnberg, 91054 Erlangen, Germany. ensser@viro.med.uni0erlangen.de
Pfinder
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Müller-Fleckenstein
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Fleckenstein
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D000818:Animals; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012342:RNA, Small Nuclear / Q000235:genetics / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000821:virology*
D012342:RNA, Small Nuclear; D012367:RNA, Viral