title: Detection of annexin I and IV and haptoglobin in bronchoalveolar lavage fluid from calves experimentally inoculated with Pasteurella haemolytica.
abstract: OBJECTIVE
  To determine whether annexins or haptoglobin could be detected in bronchoalveolar lavage (BAL) fluid specimens obtained from calves experimentally inoculated with Pasteurella haemolytica.
  
  
  METHODS
  Twelve 2- to 3-month-old male Holstein calves.
  
  
  METHODS
  Pasteurella haemolytica was inoculated into the right lung lobes of each of 6 calves. Six other calves received vehicle alone and were used as control calves. Specimens of BAL fluid were obtained from 3 control and 3 inoculated calves 1 day after inoculation and from the other calves 2 days after inoculation. The amount of annexins I, II, IV, and VI, and haptoglobin in BAL fluid specimens was examined by use of immunoblot analysis.
  
  
  RESULTS
  Annexins I and IV were detected in BAL fluid specimens obtained from the right lung lobes of each of the inoculated calves, but annexins II and VI were not. Annexin I also was found in BAL fluid specimens obtained from the left lung lobes of each inoculated calf and from left and right lung lobes of the control calves. By comparison, detection of annexin IV was essentially limited to the right lung lobes of inoculated calves. Haptoglobin was detected in some, but not all, BAL fluid specimens from the right lung lobes of inoculated calves, and its detection in BAL fluid was associated with serum proteins such as albumin.
  
  
  CONCLUSIONS
  Annexin IV was detected most specifically in response to inoculation of P haemolytica. This protein could be used as a marker for inflammatory pulmonary disease caused by P haemolytica.
authors.affiliation: Department of Systematic Diagnosis, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Katoh
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000032:analysis*; D017314:Annexin A4 / Q000032:analysis*; D000855:Anorexia / Q000209:etiology / Q000662:veterinary; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002417:Cattle; D002418:Cattle Diseases / Q000503:physiopathology*; D006242:Haptoglobins / Q000032:analysis*; D008297:Male; D016978:Mannheimia haemolytica; D010326:Pasteurella Infections / Q000503:physiopathology / Q000662:veterinary*; D012120:Respiration Disorders / Q000209:etiology / Q000662:veterinary; D013997:Time Factors
chemical_list: D017305:Annexin A1; D017314:Annexin A4; D006242:Haptoglobins