Drug-antibody conjugates with anti-HIV activity.
Human immunodeficiency virus (HIV)-specific peptide antibody-brefeldin A conjugates and antibody-glaucarubolone conjugates directed to cell surface viral glycoprotein epitopes were prepared and tested for antiviral activity. A selective response was observed both on survival of cell lines permanently infected with lentiviruses and on HIV infectivity. With human peripheral blood mononuclear cells (PBMCs), the conjugate also was effective in reducing virus titers. The effectiveness of an HIV-specific peptide antibody-brefeldin A conjugate was enhanced by combination with 3'-azido-3'-deoxythymidine (AZT) and was effective against AZT-resistant isolates in combination with AZT. The conjugates reduced virus production in MOLT-4 cells and in HIV-1-infected PBMCs without affecting the viability of uninfected cells.
Paulik
M
M
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Grieco
P
P
Kim
C
C
Maxeiner
H G
HG
Grunert
H P
HP
Zeichhardt
H
H
Morré
D M
DM
Morré
D J
DJ
D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology*; D006023:Glycoproteins / Q000276:immunology; D006678:HIV / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D015483:HIV Antibodies / Q000494:pharmacology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D006801:Humans; D018796:Immunoconjugates / Q000494:pharmacology*; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology*
D019380:Anti-HIV Agents; D006023:Glycoproteins; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D018796:Immunoconjugates; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine