title: Evaluation of three commercial serological tests with different methodologies to assess Helicobacter pylori infection.
abstract: The sera of 142 Helicobacter pylori-positive and 32 H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.
authors.affiliation: Department of Medical Microbiology, Amsterdam, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: van Der Ende
authors.forename: R W
authors.initials: RW
authors.lastname: van Der Hulst
authors.forename: P
authors.initials: P
authors.lastname: Roorda
authors.forename: G N
authors.initials: GN
authors.lastname: Tytgat
authors.forename: J
authors.initials: J
authors.lastname: Dankert
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D004724:Endoscopy; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D010437:Peptic Ulcer / Q000382:microbiology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial