Potassium current suppression in patients with peripheral nerve hyperexcitability.
Acquired neuromyotonia (Isaac's syndrome) is considered to be an autoimmune disease, and the pathomechanism of nerve hyperexcitability in this syndrome is correlated with anti-voltage-gated K(+) channel (VGKC) antibodies. The patch-clamp technique was used to investigate the effects of immunoglobulins from acquired neuromyotonia patients on VGKCs and voltage-gated Na(+) channels in a human neuroblastoma cell line (NB-1). K(+) currents were suppressed in cells that had been co-cultured with acquired neuromyotonia patients' immunoglobulin for 3 days but not for 1 day. The activation and inactivation kinetics of the outward K(+) currents were not altered by these immunoglobulins, nor did the immunoglobulins significantly affect the Na(+) currents. Myokymia or myokymic discharges, with peripheral nerve hyperexcitability, also occur in various neurological disorders such as Guillain-Barré syndrome and idiopathic generalized myokymia without pseudomyotonia. Immuno-globulins from patients with these diseases suppressed K(+) but not Na(+) currents. In addition, in hKv 1.1- and 1.6-transfected CHO (Chinese hamster ovary)-K1 cells, the expressed VGKCs were suppressed by sera from acquired neuromyotonia patients without a change in gating kinetics. Our findings indicate that nerve hyperexcitability is mainly associated with the suppression of voltage-gated K(+) currents with no change in gating kinetics, and that this suppression occurs not only in acquired neuromyotonia but also in Guillain-Barré syndrome and idiopathic generalized myokymia without pseudomyotonia.
Nagado
T
T
Third Department of Internal Medicine, Kagoshima University School of Medicine, Kagoshima, Japan.
Arimura
K
K
Sonoda
Y
Y
Kurono
A
A
Horikiri
Y
Y
Kameyama
A
A
Kameyama
M
M
Pongs
O
O
Osame
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D051668:Delayed Rectifier Potassium Channels; D005260:Female; D020275:Guillain-Barre Syndrome / Q000276:immunology / Q000503:physiopathology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology / Q000502:physiology; D015640:Ion Channel Gating; D007700:Kinetics; D051662:Kv1.1 Potassium Channel; D008297:Male; D008875:Middle Aged; D020385:Myokymia / Q000276:immunology / Q000503:physiopathology; D009222:Myotonia / Q000276:immunology / Q000503:physiopathology; D018408:Patch-Clamp Techniques; D010523:Peripheral Nervous System Diseases / Q000276:immunology / Q000503:physiopathology*; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated; D015222:Sodium Channels / Q000378:metabolism / Q000502:physiology; D014407:Tumor Cells, Cultured
D051668:Delayed Rectifier Potassium Channels; D007136:Immunoglobulins; C496946:KCNA1 protein, human; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D015222:Sodium Channels; D051662:Kv1.1 Potassium Channel