title: [Comparison of the newly developed MB redox system with mycobacteria growth indicator tube (MGIT) and 2% Ogawa egg media for recovery of mycobacteria in clinical specimens].
abstract: The rate of recovery and the mean time to detection of mycobacteria in clinical specimens were determined in a newly-developed MB Redox system based on liquid medium, and the results were compared with those of MGIT and 2% Ogawa egg media. From 587 sputum specimens processed, totally 203 mycobacterial isolates were detected, of which 177 (87.2%) with MB Redox, 185 (91.1%) with MGIT and 133 (65.6%) with 2% Ogawa medium. The difference in the percentages of positive cultures between either of the two liquid media and 2% Ogawa medium was significant (p < 0.0001). The mean time to detection of the Mycobacterium tuberculosis complex was 17.5 days with MB Redox, 18.7 days with MGIT, and 26.2 days with 2% Ogawa medium. The contamination rates were 1.5, 1.7, and 4.1% for MB Redox, MGIT, and 2% Ogawa medium, respectively. In conclusion, both MB Redox and MGIT systems, based on liquid medium, are more efficient than 2% Ogawa medium for the recovery of mycobacteria in clinical specimens.
authors.affiliation: Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo Japan.
authors.forename: C
authors.initials: C
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: E
authors.initials: E
authors.lastname: Tsubura
authors.forename: M
authors.initials: M
authors.lastname: Yamanaka
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
authors.forename: M
authors.initials: M
authors.lastname: Osumi
authors.forename: M
authors.initials: M
authors.lastname: Takeda
authors.forename: A
authors.initials: A
authors.lastname: Kurashima
authors.forename: A
authors.initials: A
authors.lastname: Yoneyama
authors.forename: K
authors.initials: K
authors.lastname: Okuzumi
mesh_terms: D001431:Bacteriological Techniques / Q000592:standards*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology; D013997:Time Factors
chemical_list: D003470:Culture Media