title: Changes in endotoxin-binding proteins during major elective surgery: important role for soluble CD14 in regulation of biological activity of systemic endotoxin.
abstract: Assessment of circulating endotoxin during the perioperative period, which is only demonstrated by the Limulus amebocyte lysate (LAL) test, may be modulated by several endotoxin-binding proteins. Endotoxin-neutralizing capacity (ENC) and the plasma levels of soluble CD14 (sCD14), lipopolysaccharide-binding protein, and bactericidal/permeability-increasing protein (BPI) were determined in 40 patients 6 h prior to skin incision for major abdominal surgery. The bioactivity of plasma endotoxin was tested by the polymyxin B-inhibited stimulatory activity of the plasma samples on healthy monocytes as measured by the release of tumor necrosis factor alpha. Plasma endotoxin levels in almost all patients increased from 0.05 +/- 0.01 to 0.23 +/- 0.03 experimental units (EU) per ml (P < 0.001); more specifically, 17 of 40 samples showed endotoxin levels of greater than 0.2 EU per ml and corresponding reductions in ENC. Soluble CD14 plasma levels were decreased from 5. 6 +/- 0.3 to 4.6 +/- 0.3 microg per ml (P < 0.05). ENC was strongly correlated with the sCD14 plasma concentration throughout the period of observation. The addition of sCD14-neutralizing monoclonal anti-sCD14 antibodies reduced ENC both pre- and postoperatively. No correlation could be established between ENC and the plasma levels of BPI, high-density lipoproteins, or low-density lipoproteins determined by measuring the concentrations of apoprotein A and apoprotein B. Biologically active endotoxin was found in only 6 of 17 samples with endotoxin levels greater than 0.2 EU per ml in the LAL test. These samples could be characterized by their perioperative loss of at least 35% of their sCD14. No change in sCD14 was detected in the remaining 11 samples. The perioperative loss of ENC is partly caused by the loss of sCD14 resulting from its consumption by endotoxin reaching the bloodstream. This study demonstrated the role of sCD14 on the bioactivity of circulating endotoxin in a human model of endotoxemia after major abdominal surgery.
authors.affiliation: Department of Surgery, The University of Tokyo, Tokyo, Japan. naki@bd.mbn.or.jp
authors.forename: N
authors.initials: N
authors.lastname: Hiki
authors.forename: D
authors.initials: D
authors.lastname: Berger
authors.forename: M A
authors.initials: MA
authors.lastname: Dentener
authors.forename: Y
authors.initials: Y
authors.lastname: Mimura
authors.forename: W A
authors.initials: WA
authors.lastname: Buurman
authors.forename: C
authors.initials: C
authors.lastname: Prigl
authors.forename: M
authors.initials: M
authors.lastname: Seidelmann
authors.forename: E
authors.initials: E
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Kaminishi
authors.forename: H G
authors.initials: HG
authors.lastname: Beger
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000209:Acute-Phase Proteins; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001054:Apolipoproteins A / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood*; D017558:Elective Surgical Procedures; D005260:Female; D006801:Humans; D007700:Kinetics; D008033:Limulus Test; D018950:Lipopolysaccharide Receptors / Q000097:blood* / Q000276:immunology*; D008070:Lipopolysaccharides / Q000032:analysis / Q000097:blood / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry / Q000276:immunology; D011112:Polymyxin B; D011184:Postoperative Period; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D001798:Blood Proteins; D002352:Carrier Proteins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011112:Polymyxin B; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D000099231:Lipopolysaccharide-Binding Protein; C021623:bactericidal permeability increasing protein