[The role of the extent of hydration of reversed micelles of surfactant s in the regulation of the peroxidase activity of ferritin and immunocomplexes of cortisol-peroxidase conjugates].
The effect of the degree of hydration (W0) of reversed micelles of Aerosol OT (AOT) and its mixture with Triton X-45 in heptane on the peroxidase activity of horse spleen ferritin in the oxidation of various substrates by hydrogen peroxide and organic hydroperoxides and on the activity of solubilized or immobilized immunocomplexes of horseradish peroxidase-cortisol conjugates (HP-COR) was studied. The peroxidase activity versus W0 plot has maxima at W0 8-14 and 19-22, which cannot be attributed to dissociation of immunocomplexes into its components or of ferritin into its subunits. The possibility of the stabilization of the conformers of oligomeric proteins by reversed micelles and the effect of the self-association of micelles on the peroxidase activity of the HP-COR immunocomplexes and ferritin were discussed. A procedure for the isolation of the iron-containing cluster from the ferritin molecule without reduction of the Fe3+ ions was suggested.
Eremin
A N
AN
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk. lando@ns.iboch.ac.by
Arapova
G S
GS
Metelitsa
D I
DI
D000818:Animals; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D005293:Ferritins / Q000737:chemistry* / Q000378:metabolism; D006736:Horses; D006854:Hydrocortisone / Q000737:chemistry* / Q000378:metabolism; D017830:Octoxynol; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry* / Q000378:metabolism; D013154:Spleen; D013379:Substrate Specificity
D004143:Dioctyl Sulfosuccinic Acid; D017830:Octoxynol; D005293:Ferritins; D010544:Peroxidases; D006854:Hydrocortisone