Induction of cyclooxygenase-2 in the rat brain after a mild episode of focal ischemia without tissue inflammation or neural cell damage.
Cyclooxygenase-2, a key enzyme in prostanoid synthesis, is induced by inflammatory stimuli and it is associated with cell death after cerebral ischemia. Here we evaluated whether cyclooxygenase-2 was induced after a short (10-min) episode of focal ischemia, mild enough not to cause inflammation or cell death. One-hour ischemia leading to brain infarct was studied for comparative purposes. Induction of cyclooxygenase-2 mRNA and protein was detected after both 10-min and 1-h ischemia. However, signs of edema were only apparent after 1-h, but not 10-min ischemia, and only rats subjected to 1-h ischemia had developed brain infarct at 4 days. Therefore, cyclooxygenase-2, not linked with neural cell death or inflammation, is induced after focal ischemia.
Planas
A M
AM
Departament de Farmacologia i Toxicologia, IIBB, CSIC-IDIBAPS, Barcelona, Spain. ampfat@cid.csic.es
Soriano
M A
MA
Justicia
C
C
Rodríguez-Farré
E
E
D000818:Animals; D001157:Arterial Occlusive Diseases; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000201:enzymology* / Q000473:pathology; D002544:Cerebral Infarction / Q000201:enzymology / Q000209:etiology / Q000473:pathology; D051546:Cyclooxygenase 2; D004790:Enzyme Induction; D007249:Inflammation / Q000473:pathology; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000473:pathology; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases