Repair of osteochondral defects with allogeneic tissue engineered cartilage implants.
The objective of this study was to evaluate the effect of allogeneic tissue engineered cartilage implants on healing of osteochondral defects. Rabbit chondrocytes were cultured in monolayer, then seeded onto biodegradable, three-dimensional polyglycolic acid meshes. Cartilage constructs were cultured hydrodynamically to yield tissue with relatively more (mature) or less (immature) hyalinelike cartilage, as compared with adult rabbit articular cartilage. Osteochondral defects in the patellar grooves of both stifle joints either were left untreated or implanted with allogeneic tissue engineered cartilage. Histologic samples from in and around the defect sites were examined 3, 6, 9, and 12, and 24 months after surgery. By 9 months after surgery, defects sites treated with cartilage implants contained significantly greater amounts of hyalinelike cartilage with high levels of proteoglycan, and had a smooth, nonfibrillated articular surface as compared to untreated defects. In contrast, the repair tissue formed in untreated defects had fibrillated articular surfaces, significant amounts of fibrocartilage, and negligible proteoglycan. These differences between treated and untreated defects persisted through 24 months after surgery. The results of this study suggest that the treatment of osteochondral lesions with allogenic tissue engineered cartilage implants may lead to superior repair tissue than that found in untreated osteochondral lesions.
Schreiber
R E
RE
Advanced Tissue Sciences, Inc., La Jolla, CA 92037-1005, USA.
Ilten-Kirby
B M
BM
Dunkelman
N S
NS
Symons
K T
KT
Rekettye
L M
LM
Willoughby
J
J
Ratcliffe
A
A
D000818:Animals; D001672:Biocompatible Materials; D001709:Biotechnology; D002358:Cartilage, Articular / Q000166:cytology / Q000601:surgery*; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D008297:Male; D011100:Polyglycolic Acid; D011817:Rabbits; D012038:Regeneration; D013264:Stifle / Q000601:surgery; D014184:Transplantation, Homologous
D001672:Biocompatible Materials; D011100:Polyglycolic Acid