title: Deletogenic activity of 1,2:7,8-diepoxyoctane in the Salmonella typhimurium tester strain TA102.
abstract: 1,2:7,8-Diepoxyoctane (DEO), whose deletogenic activity was first demonstrated in ad-3 system of Neurospora crassa and then in different species, has been tested in Salmonella typhimurium tester strain TA102 (hisG428(Ochre)). It was confirmed that it is a direct acting mutagen and was found that its activity is stimulated with the S9 mix. Obtained His(+) revertants were screened on their response to the histidine analog, N-(2-thiazolyl)-DL-alanine (ThiAla). Thirty-two percent of spontaneous and 52% of DEO-induced revertants were resistant to the analog while no resistance was observed among those induced with 4-nitroquinoline-N-oxide (4NQO). Resistance to ThiAla was interpreted as due to small deletions surrounding the target TAA codon in hisG428(Ochre). Thus, at least two simple test-systems, ad-3 of N. crassa and hisG428(Ochre) of S. typhimurium, gave compatible results and might be useful in searching of deletogens.
authors.affiliation: Departamento de Biofsica, GENOTOX-Laboratório de Genotoxicidade/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Bloco IV, Prdio 43.421, Caixa Postal 15.005, Campus do Vale/UFRGS, CEP 91501-970, Porto Alegre, RS, Brazil.
authors.forename: J N
authors.initials: JN
authors.lastname: Picada
authors.forename: N N
authors.initials: NN
authors.lastname: Khromov-Borisov
authors.forename: J A
authors.initials: JA
authors.lastname: Henriques
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D004852:Epoxy Compounds / Q000633:toxicity*; D005798:Genes, Bacterial / Q000187:drug effects / Q000528:radiation effects; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics* / Q000528:radiation effects; D017384:Sequence Deletion
chemical_list: D000477:Alkylating Agents; D004269:DNA, Bacterial; D004852:Epoxy Compounds; D009153:Mutagens; C012896:1,2,7,8-diepoxyoctane