title: Inverted ratio of m-fas/s-fas expression in early onset pauciarticular juvenile chronic arthritis.
abstract: The expression of m-fas/apo1 (CD95) and its soluble form (s-fas) was studied in patients with early onset pauciarticular (n = 23) and systemic juvenile chronic arthritis (n = 7). RNA was prepared from peripheral blood of patients and 22 healthy controls and from 23 samples derived from the synovial fluid of JCA patients. The ratio of m-fas/s-fas transcripts was calculated by densitometry of fas-specific RT-PCR products. An inverted ratio of m-fas/s-fas transcripts was found in PBMC and mononuclear cells from the joints of early onset pauciarticular JCA patients. The m-fas/s-fas ratios were similar in PBMC and mononuclear cells from synovial fluid. PBMC from patients with a systemic JCA showed the same m-fas/s-fas ratio as healthy controls, with an inverted ratio of both transcripts in cells from the synovial fluid. The data indicate a role of Fas/Apo1 regulated apoptosis in EOPA-JCA which is not limited to the affected joints.
authors.affiliation: Children's Hospital, Friedrich Alexander University, Erlangen Nuremberg, Germany.
authors.forename: J P
authors.initials: JP
authors.lastname: Haas
authors.forename: C
authors.initials: C
authors.lastname: Frank
authors.forename: R
authors.initials: R
authors.lastname: Haefner
authors.forename: M
authors.initials: M
authors.lastname: Herrmann
authors.forename: H
authors.initials: H
authors.lastname: Späth
authors.forename: H
authors.initials: H
authors.lastname: Ruder
mesh_terms: D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005786:Gene Expression Regulation; D006801:Humans; D009504:Neutrophils / Q000276:immunology; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics; D013582:Synovial Fluid / Q000276:immunology; D019014:fas Receptor / Q000235:genetics* / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D019014:fas Receptor