title: Serial analysis of gene expression in a microglial cell line.
abstract: We used the serial analysis of gene expression (SAGE) method to systematically analyze transcripts present in a microglial cell line. Over 10,000 SAGE tags were sequenced, and shown to represent 6,013 unique transcripts. Among the diverse transcripts that had not been previously detected in microglia were those for cytokines such as endothelial monocyte-activating polypeptide I (EMAP I), and for cell surface antigens, including adhesion molecules such as CD9, CD53, CD107a, CD147, CD162 and mast cell high affinity IgE receptor. In addition, we detected transcripts that were characteristic of hematopoietic cells or mesodermal structures, such as E3 protein, A1, EN-7, B94, and ufo. Furthermore, the profile contained a transcript, Hn1, that is important in hematopoietic cells and neurological development (Tang et al. Mamm Genome 8:695-696, 1997), suggesting the probable neural differentiation of microglia from the hematopoietic system in development. Messenger RNA expression of these genes was confirmed by RT-PCR in primary cultures of microglia. Significantly, this is the first systematic profiling of the genes expressed in a microglial cell line. The identification and further characterization of the genes described here should provide potential new targets for the study of microglial biology.
authors.affiliation: Division of Demyelinating Disease and Aging, National Institute of Neuroscience, Kodaira, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Sawada
authors.forename: A
authors.initials: A
authors.lastname: Ryo
authors.forename: H
authors.initials: H
authors.lastname: Tanahashi
authors.forename: T
authors.initials: T
authors.lastname: Wakatsuki
authors.forename: A
authors.initials: A
authors.lastname: Hada
authors.forename: N
authors.initials: N
authors.lastname: Kondoh
authors.forename: K
authors.initials: K
authors.lastname: Nakagaki
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Suzumura
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Tabira
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D018797:Cell Cycle Proteins; D002461:Cell Line, Transformed; D016207:Cytokines / Q000235:genetics; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000502:physiology*; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017455:Receptors, IgE / Q000235:genetics
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; D016207:Cytokines; C108200:Jpt1 protein, mouse; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; D017455:Receptors, IgE