Detection of large molecular weight cytokeratin 8 as carrier protein of CA19-9 in non-small-cell lung cancer cell lines.
It has been reported that cytokeratin 8 (CK8) is expressed in all non-small-cell lung cancers (NSCLC). We hypothesized that antigenic changes of CK8 may occur in some NSCLC cell lines. To prove this, Western immunoblot analysis using anti-human CK8 monoclonal antibodies as well as immunohistological staining of CK8 were performed in NSCLC cell lines. As a result, CK8 which had a higher molecular weight than recombinant CK8 was demonstrated in two of eight NSCLC cell lines. In addition, this CK8 contained antigenic epitopes of CA19-9. This CK8 with higher molecular weight, may have played a role in the process of invasion or metastasis of NSCLC.
Fujita
J
J
First Department of Internal Medicine, Kagawa Medical University, Kita-gun, Japan.
Dobashi
N
N
Ohtsuki
Y
Y
Ueda
Y
Y
Bandoh
S
S
Yamadori
I
I
Takahara
J
J
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D018395:CA-19-9 Antigen / Q000276:immunology / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000276:immunology / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D014407:Tumor Cells, Cultured
D018395:CA-19-9 Antigen; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D007633:Keratins