Preparation, isolation, and characterization of cutin monomers and oligomers from tomato peels.
Cutin in tomato peels was depolymerized in methanolic base to yield cutin monomers or a mixture of cutin oligomers. These products were isolated by typical solvent extraction methods or by precipitation, and the isolates were characterized by chromatographic and spectroscopic analyses. It was determined that the compositions of the isolates from both isolation procedures were similar, although solvent extraction gave higher yields. However, the precipitation method, which is easy to carry out and avoids the use of undesirable organic solvents, may be preferable in commercial processes for recovering these compounds.
Osman
S F
SF
Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA.
Irwin
P
P
Fett
W F
WF
O'Connor
J V
JV
Parris
N
N
D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D018551:Solanum lycopersicum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol; D008970:Molecular Weight
D008563:Membrane Lipids; C000521:cutin; D000432:Methanol