Activation of channel activity of the NMDA receptor-PSD-95 complex by guanylate kinase-associated protein (GKAP).
The channel-associated protein PSD-95 functionally modulates NMDA receptor channels, interacting with the channels via PDZ domain of PSD-95. PSD-95 also interacts with guanylate kinase-associated protein (GKAP) through the guanylate kinase-like domain of PSD-95. Here we report that GKAP markedly potentiates the channel activity of the receptor-PSD-95 complex. However, GKAP had no effect on basic properties of the channels nor on PSD-95-induced changes in channel properties. Thus, GKAP affects the channel activity of the NMDA receptor via PSD-95 quantitatively, which may make signal transmission more efficient at postsynaptic sites.
Yamada
Y
Y
Department of Pharmacology, Yamaguchi University School of Medicine, Ube, Japan.
Chochi
Y
Y
Ko
J A
JA
Sobue
K
K
Inui
M
M
D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D015870:Gene Expression; D018698:Glutamic Acid / Q000494:pharmacology; D007473:Ion Channels / Q000235:genetics; D008845:Microinjections; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D000074407:SAP90-PSD95 Associated Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014982:Xenopus laevis
D002352:Carrier Proteins; D007473:Ion Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D000074407:SAP90-PSD95 Associated Proteins; C033652:postsynaptic density proteins; D018698:Glutamic Acid; D013755:Tetradecanoylphorbol Acetate