title: Alpha-trifluoromethylated acyloins induce apoptosis in human oral tumor cell lines.
abstract: Cytotoxic activity of newly synthesized trifluoromethyl ketones and related compounds was studied using two human oral tumor cell lines (HSG and HSC-2). Among them, alpha-trifluoromethylacyloins (1 and 2) were found to induce apoptotic cell death, as judged by the terminal deoxynucleotidyl transferase (TdT) dUTP nick end-labeling (TUNEL) method which detects DNA nick or fragments. Furthermore, the cytoplasm of 1 or 2 treated HSG cells was stained by M30 monoclonal antibody, which detects the product resulting from the cleavage of cytokeratin 18 by activated caspase.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawase
authors.forename: H
authors.initials: H
authors.lastname: Sakagami
authors.forename: K
authors.initials: K
authors.lastname: Kusama
authors.forename: N
authors.initials: N
authors.lastname: Motohashi
authors.forename: S
authors.initials: S
authors.lastname: Saito
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology; D017611:Fluorine Compounds / Q000494:pharmacology; D006801:Humans; D020287:In Situ Nick-End Labeling; D007659:Ketones / Q000138:chemical synthesis* / Q000494:pharmacology; D009062:Mouth Neoplasms; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D005233:Fatty Alcohols; D017611:Fluorine Compounds; D007659:Ketones; C011159:acyloin; D020169:Caspases