[Biochemical characteristics of bovine retina nucleoside diphosphate kinase].
Nucleoside diphosphate kinase (NDP kinase; ATP: NDP phosphotransferase; EC 2.7.4.6) was purified from bovine retina. The molecular mass of the native enzyme was found to be 72 kDa, and those of its subunits were 17.5 and 18.5 kDa. Kinetic characteristics of the enzyme were determined. It was shown that NDP kinase exists in retina in both soluble and membrane-bound forms.
Karashchuk
G N
GN
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. karas@ibch.siobc.ras.ru
Kakuev
D L
DL
Popov
V I
VI
Gaĭdarov
I O
IO
Abdulaev
N G
NG
D000818:Animals; D002384:Catalysis; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D009701:Nucleoside-Diphosphate Kinase / Q000302:isolation & purification / Q000378:metabolism*; D012160:Retina / Q000201:enzymology*; D013379:Substrate Specificity
D009701:Nucleoside-Diphosphate Kinase