In vitro survival of Listeria monocytogenes in human amniotic fluid.
A Listeria monocytogenes strain (CA01/93), haemolytic and virulent in mice, was inoculated in 16 samples of sterile human amniotic fluid to study the growth curves and to provide more information about the pathophysiology of the microorganism in pregnancy. A control test for every analysis was performed with an Escherichia coli strain (CN 02/95). Regardless of the amniotic fluid samples were taken at 34th-39th week of gestation, when it expresses maximum ability to inhibit growth of E. coli, the growth curves of L. monocytogenes do not show any bactericidal (only bacteriostatic) activity of the amniotic fluids against the tested strain. This gives an interesting result about the pathogenesis of the infection in pregnancy and about the potential environmental contamination due to long surviving times of the microorganism in-amniotic fluid.
Altieri
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C
Alimentar Productions and Preparations Institute, Agricultural Faculty, Foggia, Italy. ippalim.fgagr@isnet.it
Maruotti
G
G
Natale
C
C
Massa
S
S
D000653:Amniotic Fluid / Q000382:microbiology*; D002585:Cesarean Section; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development / Q000472:pathogenicity; D005260:Female; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000472:pathogenicity; D008088:Listeriosis / Q000382:microbiology* / Q000517:prevention & control; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology* / Q000517:prevention & control; D011263:Pregnancy Trimester, Third