Phytomonas: analysis of polymorphism and genetic relatedness between isolates from plants and phytophagous insects from different geographic regions by RAPD fingerprints and synapomorphic markers.
The random amplification of polymorphic DNA was used for easy, quick and sensitive assessment of genetic polymorphism within Phytomonas to discriminate isolates and determine genetic relationships within the genus. We examined 48 Phytomonas spp., 31 isolates from plants and 17 from insects, from different geographic regions. Topology of the dendrogram based on randomly amplified polymorphic DNA fingerprints segregated the Phytomonas spp. into 5 main clusters, despite the high genetic variability within this genus. Similar clustering could also be obtained by both visual and cross-hybridization analysis of randomly amplified synapomorphic DNA fragments. There was some concordance between the genetic relationship of isolates and their plant tissue tropism. Moreover, Phytomonas spp. from plants and insects were grouped according to geographic origin, thus revealing a complex structure of this taxon comprising several clusters of very closely related organisms.
Serrano
M G
MG
Departamento de Parasitologia, Universidade de São Paulo, Brazil.
Camargo
E P
EP
Teixeira
M M
MM
D000818:Animals; D016172:DNA Fingerprinting; D007313:Insecta / Q000469:parasitology; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010944:Plants / Q000469:parasitology; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014351:Trypanosomatina / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification