Induction of stress proteins in human endothelial cells by heavy metal ions and heat shock.
In the present study, we compared the induction of heat shock proteins (HSPs) by heat and heavy metal ions in three different endothelial cell types, namely, human umbilical vein endothelial cells, human pulmonary microvascular endothelial cells, and the cell line EA.hy 926. Our results show that especially Zn(2+) and Cd(2+) are inducers of 70-kDa (HSP70), 60-kDa (HSP60), 32-kDa (HSP32), and 27-kDa (HSP27) HSPs. The strength of inducibility is specific for each HSP. Ni(2+) and Co(2+) only show an inducible effect at very high concentrations, that is, in the clearly cytotoxic range. Furthermore, we investigated the time course of HSP expression and the involvement of heat shock factor-1. Our study demonstrates that the three endothelial cell types that were under investigation show comparable stress protein expression when treated with heavy metal ions or heat shock. The expression of stress proteins may be used as an early marker for the toxic damage of cells. This damage can be an inducer of acute respiratory distress syndrome in which microvascular endothelial lesions occur early. Our study provides evidence that human umbilical vein endothelial cells or EA.hy 926 cells, which are much more easily isolated and/or cultivated than pulmonary microvascular endothelial cells, could be used as alternative cell culture systems for studies on cellular dysfunction in the lung caused by toxic substances, certainly with respect to the expression of HSPs.
Wagner
M
M
Institute of Pathology, University of Mainz, D-55101 Mainz, Germany.
Hermanns
I
I
Bittinger
F
F
Kirkpatrick
C J
CJ
D015153:Blotting, Western; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000032:analysis / Q000235:genetics; D004730:Endothelium, Vascular / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D018869:Heat-Shock Response / Q000502:physiology*; D006801:Humans; D019216:Metals, Heavy / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D014471:Umbilical Veins / Q000166:cytology
D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D019216:Metals, Heavy; D012333:RNA, Messenger