title: Effect of tyrosinase inhibitors on Tuber borchii mycelium growth in vitro.
abstract: This paper reports the effect of the tyrosinase (monophenol o-diphenol:oxygen oxidoreductase; EC 1.14.18.1) inhibitors diethyldithiocarbamate (DETC), L-tropolone, kojic acid, phenylthiourea (PTU) and L-mimosine on the in vitro growth of Tuber borchii (a white truffle) mycelium. A significant inhibitory effect on mycelium growth was observed for DETC, PTU and L-tropolone (0% growth compared to control at 100 microg ml(-1) DETC, PTU and L-tropolone and at 10 microg ml(-1) DETC and L-tropolone). As a comparison the action of the same inhibitors was also tested on the growth and pigmentation of the mould Cladosporium sphaerospermum. In the presence of CuSO(4) 10(-6) M T. borchii mycelium acquired pigmentation (as rounded aggregates compared to control revealed by SEM microscopy). Tyrosinase activity in the extract from T. borchii mycelium (18-day culture) was detected spectrophotometrically.
authors.affiliation: Department of Basic and Applied Biology, Faculty of Sciences, University of L'Aquila, Via Vetoio, Loc. Coppito, I-67100, L'Aquila, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Poma
authors.forename: G
authors.initials: G
authors.lastname: Pacioni
authors.forename: S
authors.initials: S
authors.lastname: Colafarina
authors.forename: M
authors.initials: M
authors.lastname: Miranda
mesh_terms: D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development; D002963:Cladosporium / Q000254:growth & development; D019327:Copper Sulfate; D003470:Culture Media / Q000737:chemistry; D004050:Ditiocarb / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D008898:Mimosine / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009183:Mycotoxins / Q000494:pharmacology; D010670:Phenylthiourea / Q000494:pharmacology; D010860:Pigments, Biological; D011753:Pyrones / Q000494:pharmacology; D014334:Tropolone / Q000494:pharmacology
chemical_list: D003470:Culture Media; D004791:Enzyme Inhibitors; D009183:Mycotoxins; D010860:Pigments, Biological; D011753:Pyrones; D008898:Mimosine; D010670:Phenylthiourea; C011890:kojic acid; D014334:Tropolone; D004050:Ditiocarb; D014442:Monophenol Monooxygenase; D019327:Copper Sulfate