title: Conservation of the pyrrolnitrin biosynthetic gene cluster among six pyrrolnitrin-producing strains.
abstract: The prnABCD gene cluster from Pseudomonas fluorescens encodes the biosynthetic pathway for pyrrolnitrin, a secondary metabolite derived from tryptophan which has strong anti-fungal activity. We used the prn genes from P. fluorescens strain BL915 as a probe to clone and sequence homologous genes from three other Pseudomonas strains, Burkholderia cepacia and Myxococcus fulvus. With the exception of the prnA gene from M. fulvus59% similar among the strains, indicating that the biochemical pathway for pyrrolnitrin biosynthesis is highly conserved. The prnA gene from M. fulvus is about 45% similar to prnA from the other strains and contains regions which are highly conserved among all six strains.
authors.affiliation: Novartis Agribusiness Biotechnology Research, P.O. Box 12257, Research Triangle Park, NC 27709, USA.
authors.forename: P E
authors.initials: PE
authors.lastname: Hammer
authors.forename: W
authors.initials: W
authors.lastname: Burd
authors.forename: D S
authors.initials: DS
authors.lastname: Hill
authors.forename: J M
authors.initials: JM
authors.lastname: Ligon
authors.forename: K
authors.initials: K
authors.lastname: van Pée
mesh_terms: D000935:Antifungal Agents / Q000096:biosynthesis; D001483:Base Sequence; D015139:Blotting, Southern; D016956:Burkholderia cepacia / Q000235:genetics; D017124:Conserved Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D016940:Myxococcus / Q000235:genetics; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism; D011764:Pyrrolnitrin / Q000096:biosynthesis*; D016415:Sequence Alignment
chemical_list: D000935:Antifungal Agents; D011764:Pyrrolnitrin