title: Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A.
abstract: Variable regulatory subunits of protein phosphatase 2A (PP2A) modulate activity, substrate selectivity and subcellular targeting of the enzyme. We have cloned a novel member of the B type regulatory subunit family, B delta, which is most highly related to B alpha. B delta shares with B alpha epitopes previously used to generate subunit-specific antibodies. Like B alpha, but unlike B beta and B gamma which are highly brain-enriched, B delta mRNA and protein expression in tissues is widespread. B delta is a cytosolic subunit of PP2A with a subcellular localization different from B alpha and may therefore target a pool of PP2A holoenzymes to specific substrates.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: S
authors.initials: S
authors.lastname: Strack
authors.forename: D
authors.initials: D
authors.lastname: Chang
authors.forename: J A
authors.initials: JA
authors.lastname: Zaucha
authors.forename: R J
authors.initials: RJ
authors.lastname: Colbran
authors.forename: B E
authors.initials: BE
authors.lastname: Wadzinski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D019556:COS Cells; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D054648:Protein Phosphatase 2; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D015398:Signal Transduction
chemical_list: D010446:Peptide Fragments; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2