The increase in the steady-state level of major histocompatibility complex mRNA in the host peripheral T lymphocytes due to ischaemia-reperfusion injury.
In our previous study, using a swine model of single lung transplantation, a relationship between the level of major histocompatibility complex (MHC II) expression on host T lymphocytes and the extent of the ex vivo preservation time was observed. Furthermore, a model of ischaemia by simple cross-clamping proved MHC II up-regulation to be independent of tissue incompatibility. The mechanism through which ischaemia-reperfusion injury (IRI) induces MHC up-regulation in host peripheral T cells has not been reported. The objective of this study was to determine whether IRI induces MHC II up-regulation in T cells by altering the intracellular steady-state level of MHC II mRNA. Group A (seven donors, seven recipients) was an allotransplantation model of 15 h of cold storage (4 degrees C) while in group B (n = 6) animals underwent 2 h of warm ischaemia. Group C (n = 6) underwent sham operation. For quantification of mRNA extracted from peripheral T lymphocytes isolated before and after surgery, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to determine the time at which mRNA levels reached its peak. The mRNA at pre-reperfusion and the time, at which mRNA peaked, was used for competitive RT-PCR. The results of RT-PCR analyses demonstrated that IRI induced an increase in the steady-state level of MHC II mRNA (p < 0.02) within 2 h post-reperfusion, irrespective of type of ischaemia and tissue incompatibility. In conclusion, this study suggested that IRI up-regulates the MHC II expression in peripheral T cells by altering the intracellular steady-state level of MHC II-DR-beta.
Nikbakht-Sangari
M
M
Department of Surgery and Medicine, University of British Columbia, Vancouver General Hospital, Canada.
Qayumi
A K
AK
Duronio
V
V
D000818:Animals; D015925:Cryopreservation; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D008168:Lung / Q000098:blood supply*; D016040:Lung Transplantation / Q000276:immunology; D009926:Organ Preservation / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D015427:Reperfusion Injury / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D006684:HLA-DR Antigens; D012333:RNA, Messenger