[Incidence of extended spectrum beta lactamase in Escherichia coli in a university hospital from 1994-1996].
BACKGROUND
The aim of this study was to evaluate the incidence of TEM- and SHV-type extended spectrum betalactamases (ESBLs) in Escherichia coli in a 700-bed teaching hospital between 1994 and 1996.


METHODS
Strains that presented reduced diameters to third-generation cephalosporins, as identified by disc diffusion techniques, were studied. The betalactamases involved were characterized by determination of the isoelectric point, hydrolysis profile, gene detection by polymerase chain reaction (PCR), and sequencing of the amplified products.


RESULTS
96 strains (1.4%) out of 7,054 strains of E. coli isolated between 1994 and 1996 showed decreased susceptibility to third-generation cephalosporins and only 4 strains (0.06%) produced ESBLs. Two strains produced SHV-2 and two produced TEM-12.


CONCLUSIONS
The contribution of ESBL production to resistance to third-generation cephalosporins was low: 0.06% of all the E. coli strains isolated between 1994 and 1996.
Sabaté
M
M
Servicio de Microbiología, Hospital de la Santa Creu i Sant Pau, Barcelona.
Vergés
C
C
Miró
E
E
Mirelis
B
B
Navarro
F
F
del Río
E
E
Prats
G
G
D004926:Escherichia coli / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000453:epidemiology*; D006785:Hospitals, University; D006801:Humans; D007526:Isoelectric Point; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000032:analysis*
D001618:beta-Lactamases