Genetic diversity of Chinese native pigs inferred from protein electrophoresis.
We examined protein polymorphism of 20 native pig breeds in China and 3 introduced pig breeds. Thirty loci have been investigated, among which six loci were found to be polymorphic. Especially, the polymorphism of malate dehydrogenase (MDH), adenylate kinase (AK), and two new alleles of adenosine deaminase (ADA) had not been reported in domestic pigs and wild pigs. The percentage of polymorphic loci (P), the mean heterozygosity (H), and the mean number of alleles (A) are 0.200, 0.065, and 1.300, respectively. The degree of genetic variability of Chinese pigs as a whole was higher than that of goats, lower than that of cattle and horses, and similar to that of sheep. Using the gene frequencies of the 30 loci, Nei's genetic distance among the 20 native breeds in China and 3 introduced pig breeds was calculated by the formula of Nei. The program NEIGHBOR in PHYLIP 3.5c was chosen to construct an UPGMA tree and a NJ tree. Our results show that, of the total genetic variation found in the native pig breeds in China, 31% (0.31) is ascribable to genetic differences among breeds. About 69% of the total genetic variation is found within breeds. Most breeds are in linkage disequilibrium. The patterns of genetic similarities between the Chinese native pig breeds were not in agreement with the proposed pig type classification.
Wu
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Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, China.
Ding
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B
Zhang
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D000243:Adenosine Deaminase / Q000235:genetics; D000263:Adenylate Kinase / Q000235:genetics; D000626:Aminopeptidases / Q000235:genetics; D000818:Animals; D001947:Breeding; D002681:China; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D004586:Electrophoresis / Q000379:methods; D005787:Gene Frequency; D014644:Genetic Variation; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D006579:Heterozygote; D008291:Malate Dehydrogenase / Q000235:genetics; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D011506:Proteins; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D000263:Adenylate Kinase; D000626:Aminopeptidases; C026045:tripeptide aminopeptidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D000243:Adenosine Deaminase; D005956:Glucose-6-Phosphate Isomerase