Partitioning and concentrating biomaterials in aqueous phase systems.
Aqueous phase separation is a general phenomenon which occurs when structurally distinct water-soluble macromolecules are dissolved, above certain concentrations, in water. The number of aqueous phases obtained depends on the number of such distinct macromolecular species used. Aqueous two-phase systems, primarily those containing poly(ethylene glycol) and dextran, have been widely used for the separation of biomaterials (macromolecules, membranes, organelles, cells) by partitioning. The polymer and salt compositions and concentrations chosen greatly affect the physical properties of the phases. These, in turn, interact with the physical properties of biomaterials included in the phases and affect their partitioning. Specific extractions of biomaterials can be effected by including affinity ligands in the systems. The phase systems can also be used to obtain information on the surface properties of materials partitioned in them; to study interactions between biomaterials; and to concentrate such materials.
Johansson
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G
Department of Biochemistry, University of Lund, Sweden.
Walter
H
H
D000818:Animals; D001672:Biocompatible Materials / Q000302:isolation & purification*; D002458:Cell Fractionation; D002469:Cell Separation; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances; D009696:Nucleic Acids / Q000302:isolation & purification; D011108:Polymers; D011506:Proteins / Q000302:isolation & purification; D014867:Water
D001672:Biocompatible Materials; D008024:Ligands; D046911:Macromolecular Substances; D009696:Nucleic Acids; D011108:Polymers; D011506:Proteins; D014867:Water