Allelotype of uterine leiomyomas.
Uterine leiomyomas are the most common benign tumor that arise from smooth muscle cells of the myometrium. Little is known about the etiology and pathogenesis of this tumor. To investigate the molecular pathogenesis of these tumors, we have conducted an allelotype of 102 leiomyomas from 12 patients, using 67 fluorescently-tagged oligonucleotide primers amplifying microsatellite loci covering all autosomes. No areas of the genome showed frequent loss of heterozygosity (LOH); however, the highest rate of LOH (9%) was observed on 7q, consistent with previous cytogenetic observations. Uterine leiomyomas are sometimes multiple. In general, multiplicity of other types of neoplasm is associated with genetic predisposition to the disease. Because multiple tumors were available from each of the 12 patients studied, we looked for evidence of allele-specific LOH, which might indicate the presence of an underlying predisposition gene. However, no evidence for allele-specific LOH was detected, indicating that if cases of multiple uterine leiomyoma are due to an underlying predisposition gene, it is unlikely to be a recessive oncogene.
Mao
X
X
Section of Cancer Genetics, Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, United Kingdom.
Barfoot
R
R
Hamoudi
R A
RA
Easton
D F
DF
Flanagan
A M
AM
Stratton
M R
MR
D000483:Alleles; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics*; D018895:Microsatellite Repeats; D014594:Uterine Neoplasms / Q000235:genetics*