title: Molecular cloning of vacuolar H(+)-pyrophosphatase and its expression during the development of pear fruit.
abstract: The cDNA of vacuolar proton-translocating inorganic pyrophosphatase was cloned from pear fruit. It contained an open reading frame of 2,301 nucleotides, coding for a polypeptide of 767 amino acids. The level of mRNA was very low in young fruit and increased with maturation, differing from the changes in the level of polypeptide.
authors.affiliation: Laboratory of Horticultural Science, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Maeshima
authors.forename: S
authors.initials: S
authors.lastname: Yamaki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005638:Fruit / Q000201:enzymology* / Q000235:genetics; D043564:Inorganic Pyrophosphatase; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000235:genetics*; D014617:Vacuoles / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase