title: Compartive study of volatile components and fatty acids of plants and in vitro cultures of parsley (Petroselinum crispum (Mill) nym ex hill).
abstract: Volatile compounds from plants, callus tissue cultures, and cell suspensions of parsley (Petroselinum crispum) were captured during the growth cycle using a dynamic headspace extraction and were identified by gas chromatography-mass spectrometry. Parsley plants were found to produce mainly monoterpenes, and the compound of major abundance was p-1,3,8-menthatriene, followed by beta-phellandrene and apiole. Callus cultures and cell suspensions produced aldehydes (nonanal and decanal) that were also detected in parsley plant. The former also produced limonene, acetophenone, and benzotiazol; these were not observed in the plants. The production of volatiles in plants, callus tissue, and cell suspensions was found to be time-dependent. Free and bound fatty acids were also monitored by an in situ method. Palmitic (C16:0) and stearic (C18:0) acids were the most abundant fatty acids in all materials; however, higher levels were found in plants. On the other hand, the unsaturated C16:1 and C16:3 were not detected in the in vitro cultures.
authors.affiliation: Unidad de Biotecnología e Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-Unidad Irapuato), Irapuato, Gto., Mexico. mlopez@irapuato.ira.cinvestav.mx
authors.forename: M G
authors.initials: MG
authors.lastname: López
authors.forename: I R
authors.initials: IR
authors.lastname: Sánchez-Mendoza
authors.forename: N
authors.initials: N
authors.lastname: Ochoa-Alejo
mesh_terms: D000447:Aldehydes / Q000032:analysis; D019661:Apiaceae / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009924:Organ Culture Techniques; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D013729:Terpenes / Q000032:analysis*; D014835:Volatilization
chemical_list: D000447:Aldehydes; D005227:Fatty Acids; D013729:Terpenes