title: Transcriptional analysis of the rubrerythrin and superoxide dismutase genes of Clostridium perfringens.
abstract: We cloned and sequenced a 2.7-kb fragment of chromosomal DNA from Clostridium perfringens containing the superoxide dismutase-encoding gene, sod. Previously, rubrerythrin from C. perfringens had been isolated and its gene (rbr) had been cloned (Y. Lehmann, L. Meile, and M. Teuber, J. Bacteriol. 178:7152-7158, 1996). Northern blot experiments revealed a length of approximately 800 bases for each transcript of rbr and sod of C. perfringens. Thus, rbr and sod each represent a monocistronic operon. Their transcription start points were located by primer extension analyses. sod transcription was shown to depend on the growth phase, and it reached a maximum during the transition from log phase to stationary phase. Neither sod nor rbr transcription was influenced by oxidative stress.
authors.affiliation: Laboratorium für Lebensmittel-Mikrobiologie, Institut für Lebensmittelwissenschaft, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland.
authors.forename: T A
authors.initials: TA
authors.lastname: Geissmann
authors.forename: M
authors.initials: M
authors.lastname: Teuber
authors.forename: L
authors.initials: L
authors.lastname: Meile
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005288:Ferredoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D006422:Hemerythrin; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012416:Rubredoxins; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D005288:Ferredoxins; D006422:Hemerythrin; D012416:Rubredoxins; C055857:rubrerythrins; D013482:Superoxide Dismutase