Re-evaluation of plant sulpholipid labelling from UDP-[14C]glucose in pea chloroplasts.
The sulphoquinovosyldiacylglycerol (sulpholipid) zone obtained by TLC of pea chloroplast lipids yields, on hydrolysis, not only sulphoquinovose but also galactose and glucose. Following incorporation from UDP-[(14)C]glucose, the percentages of the total radioactivity in these three sugars were typically 1, 5 and 85%, respectively. The occurrence of the glucolipid causes difficulties in elucidating further the biosynthetic pathway for sulphoquinovose.
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School of Biosciences, Cardiff University, Cardiff CF1 3US, U.K.
Harwood
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JL
D002250:Carbon Radioisotopes; D002736:Chloroplasts / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis / Q000378:metabolism*; D018532:Pisum sativum / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006017:Glycolipids; C013694:sulfoquinovosyl diglyceride; D005947:Glucose; D014532:Uridine Diphosphate Glucose; D005690:Galactose