Extracellular lipid peroxidation of selective white-rot fungus, Ceriporiopsis subvermispora.
Ceriporiopsis subvermispora is capable of decomposing lignin without penetration of enzymes into wood cell walls. To elucidate the mechanism of lignolysis at a site far from enzymes, peroxidation of low molecular mass compounds produced by this fungus was analyzed. C. subvermispora produced free 9,12-octadecadienoic, 9-octadecenoic, 11-octadecenoic, hexadecanoic and octadecanoic acids, predominantly at an early stage of cultivation on wood meal cultures. In prolonged cultivation period after 2 weeks, the amount of intact fatty acids decreased with increasing organic hydroperoxide and TBARS production. These results suggest that lignin degradation by C. subvermispora is related to extracellular lipid peroxidation.
Enoki
M
M
Laboratory of Biomass Conversion, Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto, Japan.
Watanabe
T
T
Nakagame
S
S
Koller
K
K
Messner
K
K
Honda
Y
Y
Kuwahara
M
M
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D005227:Fatty Acids / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D015227:Lipid Peroxidation; D013058:Mass Spectrometry; D010544:Peroxidases / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances
D005227:Fatty Acids; D017392:Thiobarbituric Acid Reactive Substances; D008031:Lignin; D006861:Hydrogen Peroxide; D010544:Peroxidases; C051129:manganese peroxidase