Mucin core protein expression in extrahepatic bile duct carcinoma is associated with metastases to the liver and poor prognosis.
BACKGROUND
Curative resection does not guarantee long term survival for the patient with extrahepatic bile duct carcinoma because of the possibility of metastases to the liver (LM). Expression of mucin core protein-1 (MUC1), sialyl-Le(x), and sialosyl-Tn in bile duct carcinoma was determined and compared with LM and prognosis.


METHODS
Immunohistochemical expression of MUC1, sialyl-Le(x), and sialosyl-Tn in 73 extrahepatic bile duct tumors was analyzed using the DF3, FH6, and TKH2 monoclonal antibodies, respectively. Scoring was based on the percentage of immunoreactive cells: negative, low expression (</=25% immunoreactive cells), and high expression (>25%).


RESULTS
High expression of MUC1, sialyl-Le(x), and sialosyl-Tn was observed in 68.5%, 34.2%, and 54.8%, respectively. of 73 cases. Patients with tumors showing high expression of MUC1 had a higher rate of LM (48.9%) and a significantly shorter survival period (median survival time, 17.8 months) compared with patients with tumors showing low (incidence of LM, 9.1%; median survival time, >100 months) or negative (incidence of LM, 11.1%; median survival time, 52.9 months) expression of MUC1 (P < 0.01). However, the survival period of patients with tumors showing high, low, or negative expression of sialyl-Le(x) or of sialosyl-Tn did not differ significantly. High MUC1 expression correlated with LM by logistic regression analysis and emerged as an independent prognostic factor in stepwise multivariate analysis.


CONCLUSIONS
The results of the current study demonstrate that high expression of MUC1 correlates with LM and poor outcome in patients with extrahepatic bile duct carcinoma.
Takao
S
S
First Department of Surgery, Kagoshima University School of Medicine, Kagoshima, Japan.
Uchikura
K
K
Yonezawa
S
S
Shinchi
H
H
Aikou
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis; D001650:Bile Duct Neoplasms / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D016015:Logistic Models; D008875:Middle Aged; D018396:Mucin-1 / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009844:Oligosaccharides / Q000096:biosynthesis; D010446:Peptide Fragments / Q000096:biosynthesis*; D011379:Prognosis; D012189:Retrospective Studies; D000080506:Sialyl Lewis X Antigen
D015295:Antigens, Tumor-Associated, Carbohydrate; D006023:Glycoproteins; C116055:MUC1 tandem repeat peptide; D018396:Mucin-1; D009363:Neoplasm Proteins; D009844:Oligosaccharides; D010446:Peptide Fragments; D000080506:Sialyl Lewis X Antigen; C055491:sialosyl-Tn antigen