title: Ivermectin in reindeer feces: determination by HPLC.
abstract: An assay method for the determination of ivermectin in reindeer feces was developed. Ivermectin was quantified by high-performance liquid chromatography and fluorescence detection after extraction (acetone, isooctane), cleanup (C-18 solid-phase extraction column), and derivatization to a fluorescent derivative using N-methylimidazole and trifluoroacetic anhydride. Concentration calculations were based on calibration lines found from analyses of standards prepared in feces. Abamectin was used as internal standard. Ivermectin was determined over a wide concentration range in a single sample preparation and HPLC run for each sample. The recovery from fortified samples was greater than 95% over the concentration range 5-2000 ng/g wet weight feces.
authors.affiliation: Department of Arctic Veterinary Medicine, The Norwegian School of Veterinary Science, Stakkevollveien 23B, N-9005 Tromso, Norway. Kjetil.Aasbakk@veths.no
authors.forename: K
authors.initials: K
authors.lastname: Asbakk
authors.forename: H R
authors.initials: HR
authors.lastname: Bendiksen
authors.forename: A
authors.initials: A
authors.lastname: Oksanen
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000737:chemistry*; D007559:Ivermectin / Q000032:analysis*; D008297:Male; D012053:Reindeer; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D007559:Ivermectin