The major acidic glycolipids from the kidney of the Pacific salmon (Oncorhynchus keta): characterization of a novel ganglioside, fucosyl-N-acetylgalactosaminyl-GM1.
Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (X1) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methylation analysis, chemical and enzymatic degradation, reaction with monoclonal antibodies, LSIMS, and (1)H-NMR spectroscopy, X1 was determined to be a monosialosyl ganglioside belonging to the ganglio-series with a unique Fucalpha1-3GalNAc linkage at the nonreducing terminal: Fucalpha1-3GalNAcbeta1-3Galbeta1-3GalNAcbeta1-4[ NeuAcalpha2-3]Galbeta 1-4Glcbeta1-1Cer. Analysis of the lipophilic moiety indicated predominance of 24:1 fatty acid in combination with sphingenine. X2 was found to have a glycon structure identical to X1. The ceramide of X2 consisted predominantly of saturated fatty acids (18:0 and 16:0). The tissue concentrations of X1 and X2 in kidney were 3.7 and 2.8 nmol/g, respectively.
Niimura
Y
Y
Radioisotope Research Center, Teikyo University School of Medicine, Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Tomori
M
M
Tadano-Aritomi
K
K
Toida
T
T
Ishizuka
I
I
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D005732:Gangliosides / Q000737:chemistry / Q000302:isolation & purification; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification; D007668:Kidney / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017685:Oncorhynchus keta / Q000378:metabolism*
D005732:Gangliosides; D006017:Glycolipids; C403721:fucosyl-N-acetylgalactosaminyl-GM1; D005677:G(M1) Ganglioside