[Comparative study of two methods for salvaging red blood cells: centrifugation techniques (cell-saver) versus filtration through a 100,000-dalton filter].
OBJECTIVE
Some loss of blood occurs during blood salvage. We hypothesized that plasmapheresis filtering would damage blood much less than centrifugation techniques do, thereby allowing more red blood cells to be transfused.


METHODS
Laboratory study in which 16 units of whole donor blood were distributed randomly in two groups and processed either by a conventional "cell-saver" method or by hemofiltration using recirculation through a 100,000 dalton filter. We analysed hemoglobin, hematocrit, free hemoglobin, extracellular potassium, platelets, leukocytes, protein and albumin in whole blood before and after processing, and in the waste bag in each group.


RESULTS
The recovery of hemoglobin and red blood cell volume was about 80% with both methods. More free plasma hemoglobin was found in the waste bag with the filtration technique. Hemolysis in processed blood was low, less than 0.1% in both groups. Platelet recovery with conventional centrifugation and filtration was 11 and 49%, respectively. Albumin, total protein and extra-cellular potassium were recovered at a rate of about 20% with the filtration technique, whereas recovery of these elements was minimal with the cell saver method.


CONCLUSIONS
Both methods of autotransfusion caused moderate loss of red blood cells and low plasma levels of free hemoglobin in processed blood. Recovery of platelets, albumin, total protein and potassium was better with filtration than with the "cell-saver" method.
Catalá Bauset
J C
JC
Servicio de Anestesia-Reanimación y Terapia del Dolor, Hospital General Universitario de Valencia.
de Andrés Ibáñez
J
J
Grau Real
F
F
Bengtsson
A
A
Bengtson
J P
JP
D001782:Blood Donors; D001804:Blood Transfusion, Autologous / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes; D006440:Hemofiltration / Q000295:instrumentation / Q000379:methods; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D011897:Random Allocation; D014461:Ultracentrifugation / Q000295:instrumentation / Q000379:methods
D006454:Hemoglobins