La protein is required for efficient translation driven by encephalomyocarditis virus internal ribosomal entry site.
Translation of internal ribosomal entry site (IRES)-dependent mRNAs is mediated by RNA-binding proteins as well as canonical translation factors. In order to elucidate the roles of RNA-binding proteins in IRES-dependent translation, the role of polypyrimidine tract-binding protein (PTB) and La protein in encephalomyocarditis virus (EMCV) IRES-dependent translation was investigated. PTB was required for efficient EMCV IRES-driven translation but, intriguingly, an excess of PTB suppressed it. Such a translational suppression by surplus PTB was relieved by addition of La protein. A possible role for La protein in IRES-dependent translation is discussed.
Kim
Yoon Ki
YK
Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, Korea1.
Jang
Sung Key
SK
Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, Korea1.
D001324:Autoantigens / Q000378:metabolism*; D001667:Binding, Competitive; D015153:Blotting, Western; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004686:Encephalomyocarditis virus / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D038941:Polypyrimidine Tract-Binding Protein; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014158:Transcription, Genetic; D000097585:SS-B Antigen
D001324:Autoantigens; D004268:DNA-Binding Proteins; D038941:Polypyrimidine Tract-Binding Protein; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen