A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase.
The Bacillus subtilis ResD-ResE two-component signal transduction system is essential for aerobic and anaerobic respiration. A spontaneous suppressor mutant that expresses ResD-controlled genes and grows anaerobically in the absence of the ResE histidine kinase was isolated. In addition, aerobic expression of ResD-controlled genes in the suppressed strain was constitutive and occurred at a much higher level than that observed in the wild-type strain. The suppressing mutation, which mapped to pgk, the gene encoding 3-phosphoglycerate kinase, failed to suppress a resD mutation, suggesting that the suppressing mutation creates a pathway for phosphorylation of the response regulator, ResD, which is independent of the cognate sensor kinase, ResE. The pgk-1 mutant exhibited very low but measurable 3-phosphoglycerate kinase activity compared to the wild-type strain. The results suggest that accumulation of a glycolytic intermediate, probably 1, 3-diphosphoglycerate, is responsible for the observed effect of the pgk-1 mutation on anaerobiosis of resE mutant cells.
Nakano
M M
MM
Department of Biochemistry, Louisiana State University Medical Center, Shreveport, Louisiana 71130, USA. mnakano@bmb.ogi.edu
Zhu
Y
Y
Haga
K
K
Yoshikawa
H
H
Sonenshein
A L
AL
Zuber
P
P
D000332:Aerobiosis; D000693:Anaerobiosis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D006019:Glycolysis / Q000235:genetics; D000071677:Histidine Kinase; D015151:Immunoblotting; D009154:Mutation; D010735:Phosphoglycerate Kinase / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014157:Transcription Factors
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C100386:ResD protein, Bacillus subtilis; D014157:Transcription Factors; D011494:Protein Kinases; D000071677:Histidine Kinase; D010735:Phosphoglycerate Kinase