title: Production of extensive chickpea (Cicer arietinum L.) protein hydrolysates with reduced antigenic activity.
abstract: Chickpea protein isolate was used as starting material for the production of hypoallergenic protein hydrolysates. Western blotting of the protein isolate showed that IgE in sensitized patient sera strongly bound to the basic polypeptidic chains and recognized the acidic ones of 11S globulin. During the hydrolysis process by the individual and/or sequential action of endo- and exoproteases, a high reduction of antigenic activity was observed. Results suggest that the presence of intact or partially hydrolyzed basic polypeptide chains of 11S globulin are responsible for the formation of IgE complexes in protein hydrolysates obtained by exoprotease treatment; however, the digestion of these polypeptide chains by individual action of endoprotease caused a high loss of antigenic activity. The most effective reduction of antigenicity, >90%, was observed in extensive hydrolyzed chickpea proteins obtained by sequential treatment with endo- and exopeptidases. This chickpea protein hydrolysate could be useful for the elaboration of specialized hypoallergenic food products.
authors.affiliation: Departamento de Fisiología y Tecnología de Productos Vegetales, Instituto de la Grasa (CSIC), Avenida Padre García Tejero 4, 41012 Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Clemente
authors.forename: J
authors.initials: J
authors.lastname: Vioque
authors.forename: R
authors.initials: R
authors.lastname: Sanchez-Vioque
authors.forename: J
authors.initials: J
authors.lastname: Pedroche
authors.forename: F
authors.initials: F
authors.lastname: Millán
mesh_terms: D015153:Blotting, Western; D010450:Endopeptidases; D007887:Fabaceae; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E; D010940:Plant Proteins / Q000276:immunology*; D010946:Plants, Medicinal; D011492:Protein Hydrolysates / Q000276:immunology*
chemical_list: D010940:Plant Proteins; D011492:Protein Hydrolysates; D007073:Immunoglobulin E; D010450:Endopeptidases