Synthesis of heparin partial structures and their binding activities to platelets.
A synthetic pentasaccharide corresponding to the antithrombin III-binding region in heparin was also found to bind to human platelets. To identify the platelet-binding site in the pentasaccharide which is expected to be a novel sequence in heparin responsible for its platelet-binding, five partial structures of this particular pentasaccharide were synthesized. In a competitive assay using [3H]-heparin, a trisaccharide, O-(2-deoxy-2-sulfamido-3,6-di-O-sulfo-alpha-D-glucopyranosyl)-1--> 4)-O-(2-O-sulfo-alpha-L-idopyranosyluronic acid)-(1-->4)-2-deoxy-2-sulfamido-6-O-sulfo-alpha-D-glucopyranose, was concluded to be a high-affinity site for heparin's binding to platelets.
Koshida
S
S
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Japan.
Suda
Y
Y
Sobel
M
M
Ormsby
J
J
Kusumoto
S
S
D000990:Antithrombin III / Q000737:chemistry; D001665:Binding Sites; D001667:Binding, Competitive; D001792:Blood Platelets / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000494:pharmacology; D011485:Protein Binding
D009844:Oligosaccharides; D000990:Antithrombin III; D006493:Heparin