title: Antioxidative phenolic compounds from Japanese barnyard millet (Echinochloa utilis) grains.
abstract: Three antioxidative phenolic compounds, one serotonin derivative and two flavonoids, were isolated from an ethanol extract of Japanese barnyard millet (cv. Kurohie) grains by Sephadex LH-20 chromatography and preparative high-performance liquid chromatography. Their structures were established to be N-(p-coumaroyl)serotonin, luteolin, and tricin on the basis of spectrometric data from (1)H and (13)C and two-dimensional nuclear magnetic resonance techniques, fast atom bombardment mass spectrometry, and Fourier transform infrared spectrophotometry. N-(p-Coumaroyl)serotonin exhibited a strong antioxidant activity almost equivalent to that of butylated hydroxyanisole at the same concentration (w/v). Although the antioxidant activity of luteolin was lower than that of N-(p-coumaroyl)serotonin, it was nearly equal to that of quercetin, whereas the activity of tricin was lower than that of luteolin. All of them were newly isolated from Japanese barnyard millet grains.
authors.affiliation: Tohoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Akahira, Shimokuriyagawa, Morioka, Iwate 020-0198, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D007564:Japan; D009682:Magnetic Resonance Spectroscopy; D008897:Panicum / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D010636:Phenols