Antipeptide Antibodies as Analytical Tools To Discriminate among Bovine alpha(s1)-Casein Components.
Polyclonal antibodies raised against synthetic peptides reproducing sequence stretches of bovine alpha(s1)-casein were used as probes to discriminate within the alpha(s1)-casein fraction of bovine milk and cheese. A minor alpha(s1)-casein component, selectively recognized by an antisera directed against the bovine 139-149 alpha(s1)-casein sequence, was found to be a C-terminally truncated alpha(s1)-casein form. This component coeluted with the main alpha(s1)- and alpha(s2)-casein by anion-exchange chromatography of whole casein, whereas by RP-HPLC it eluted with alpha(s2)-casein only. Similarly to the main alpha(s1)-casein, the C-terminally truncated form was hydrolyzed in vitro by chymosin and early in the cheese-making.
Pizzano
R
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, Parco Gussone, I-80055 Portici, Italy, and Istituto Sperimentale Lattiero-Caseario, via A. Lombardo 11, I-20075 Lodi, Italy.
Nicolai
MA
Addeo
F