Ginkgo biloba inhibits hydrogen peroxide-induced activation of nuclear factor kappa B in vascular endothelial cells.
The present study determined the effects of Ginkgo biloba extract (GBE) on the activation of nuclear factor kappa B (NF-kappaB) and the level of hydrogen peroxide (H2O2) in bovine pulmonary artery endothelial cells (PAEC). H2O2 showed a concentration-dependent activation of NF-kappaB. GBE demonstrated a concentration-dependent suppression of NF-kappaB activated by H2O2. GBE directly scavenged H2O2 in a cell-free system; it also decreased H2O2 levels in PAEC. These results suggest that the inhibitory effect of GBE on H2O2-induced NF-kappaB activation may be caused by its scavenging and suppression of H2O2. Our experiments demonstrate that GBE can inhibit NF-kappaB activation induced by H2O2 and may thus be effective for the prevention or treatment of atherosclerosis and other disorders related to NF-kappaB activation.
Wei
Z
Z
Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, CA, USA.
Peng
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Q
Lau
B H
BH
Shah
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V
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology; D004789:Enzyme Activation; D020441:Ginkgo biloba / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D011651:Pulmonary Artery / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D016328:NF-kappa B; D010936:Plant Extracts; D006861:Hydrogen Peroxide