title: Genotypic diversity among Brevibacillus laterosporus strains.
abstract: In comparison with other entomopathogenic Bacillus species, the genome of Brevibacillus laterosporus is poorly characterized. The aim of this study was to examine genetic variability in B. laterosporus by using a range of typing methodologies. Strains of B. laterosporus were examined for variation in 13 chromosomal genes encoding enzymes by multilocus enzyme electrophoresis. Optimal conditions of pulsed-field gel electrophoresis and randomly amplified polymorphic DNA were established that allowed analysis of the genome of B. laterosporus. None of these techniques allowed the identification of a convenient molecular marker for entomopathogenic strains, although one specific primer amplified only DNA from almost all mosquitocidal strains.
authors.affiliation: Laboratory of Systematic Biochemistry, Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute-FIOCRUZ, Rio de Janeiro, RJ, Brazil. vzahner@gene.dbbm.fiocruz.br
authors.forename: V
authors.initials: V
authors.lastname: Zahner
authors.forename: L
authors.initials: L
authors.lastname: Rabinovitch
authors.forename: P
authors.initials: P
authors.lastname: Suffys
authors.forename: H
authors.initials: H
authors.lastname: Momen
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004798:Enzymes / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004798:Enzymes; D005819:Genetic Markers