Detection of borreliacidal antibodies in Lyme borreliosis patient sera containing antimicrobial agents.
The borreliacidal-antibody test has been used for the serological detection and confirmation of Lyme borreliosis. However, the presence of antimicrobial agents in serum can confound the accurate detection of borreliacidal antibodies. In this study, we developed a Bacillus subtilis agar diffusion bioassay to detect small concentrations of antimicrobial agents in serum. We also used XAD-16, a nonionic polymeric resin, to adsorb and remove high concentrations of amoxicillin, cefotaxime, ceftriaxone, cefuroxime, doxycycline, and erythromycin without significantly affecting even small concentrations of immunoglobulin M (IgM) or IgG borreliacidal antibodies. High concentrations of penicillin could also be removed by adding 1 U of penicillinase without significantly influencing the levels of borreliacidal antibodies. These simple procedures greatly enhance the clinical utility of the borreliacidal-antibody test.
Jobe
D A
DA
Microbiology Research Laboratory, Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601, USA.
Rawal
N
N
Schell
R F
RF
Callister
S M
SM
D000658:Amoxicillin / Q000097:blood* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000097:blood / Q000494:pharmacology; D000907:Antibodies, Bacterial / Q000097:blood*; D015153:Blotting, Western; D002439:Cefotaxime / Q000097:blood / Q000494:pharmacology; D002443:Ceftriaxone / Q000097:blood / Q000494:pharmacology; D002444:Cefuroxime / Q000097:blood / Q000494:pharmacology; D002511:Cephalosporins / Q000097:blood / Q000494:pharmacology; D004318:Doxycycline / Q000097:blood / Q000494:pharmacology; D004917:Erythromycin / Q000097:blood / Q000494:pharmacology; D005189:False Positive Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D066298:In Vitro Techniques; D008193:Lyme Disease / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology*; D010406:Penicillins / Q000097:blood / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D002511:Cephalosporins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010406:Penicillins; D004917:Erythromycin; D002443:Ceftriaxone; D000658:Amoxicillin; D004318:Doxycycline; D002439:Cefotaxime; D002444:Cefuroxime