Effects of garlic on 7,12-Dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.
The inhibitory effect of garlic (Allium sativum Linn) on 7, 12-dimethylbenz[a]anthracene (DMBA)-induced buccal pouch carcinogenesis was investigated in male Syrian hamsters. Measurement of lipid peroxidation, reduced glutathione (GSH), glutathione peroxidase (GPx), and glutathione S-transferase (GST) was used to monitor the chemopreventive potential of garlic. All hamsters painted on their buccal pouches with DMBA alone for 14 weeks developed well-differentiated squamous cell carcinomas. Diminished lipid peroxidation in the oral tumor tissue was accompanied by a significant increase in the levels of GSH, GPx, and GST. Administration of 250 mg/kg body weight aqueous garlic extract three times a week for 14 weeks effectively suppressed DMBA-induced oral carcinogenesis as revealed by the reduced incidence of neoplasms. The results of the present study suggest that garlic may exert its chemopreventive effects by modulating lipid peroxidation and enhancing the levels of GSH, GPx, and GST.
Balasenthil
S
S
Department of Biochemistry, Annamalai University, Tamil Nadu, India.
Arivazhagan
S
S
Ramachandran
C R
CR
Nagini
S
S
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D002610:Cheek; D006224:Cricetinae; D005737:Garlic; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D016896:Treatment Outcome
D002273:Carcinogens; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D015127:9,10-Dimethyl-1,2-benzanthracene; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione