title: Role of the C-terminal helix 9 in the stability and ligandin function of class alpha glutathione transferase A1-1.
abstract: Helix 9 at the C-terminus of class alpha glutathione transferase (GST) polypeptides is a unique structural feature in the GST superfamily. It plays an important structural role in the catalytic cycle. Its contribution toward protein stability/folding as well as the binding of nonsubstrate ligands was investigated by protein engineering, conformational stability, enzyme activity, and ligand-binding methods. The helix9 sequence displays an unfavorable propensity toward helix formation, but tertiary interactions between the amphipathic helix and the GST seem to contribute sufficient stability to populate the helix on the surface of the protein. The helix's stability is enhanced further by the binding of ligands at the active site. The order of ligand-induced stabilization increases from H-site occupation, to G-site occupation, to the simultaneous occupation of H- and G-sites. Ligand-induced stabilization of helix9 reduces solvent accessible hydrophobic surface by facilitating firmer packing at the hydrophobic interface between helix and GST. This stabilized form exhibits enhanced affinity for the binding of nonsubstrate ligands to ligandin sites (i.e., noncatalytic binding sites). Although helix9 contributes very little toward the global stability of hGSTA1-1, its conformational dynamics have significant implications for the protein's equilibrium unfolding/refolding pathway and unfolding kinetics. Considering the high concentration of reduced glutathione in human cells (about 10 mM), the physiological form of hGSTA1-1 is most likely the thiol-complexed protein with a stabilized helix9. The C-terminus region (including helix9) of the class alpha polypeptide appears not to have been optimized for stability but rather for catalytic and ligandin function.
authors.affiliation: Protein Structure-Function Research Program, Department of Biochemistry, University of the Witwatersrand, Johannesburg 2050, South Africa. 089dirr@cosmos.wits.ac.za
authors.forename: H W
authors.initials: HW
authors.lastname: Dirr
authors.forename: L A
authors.initials: LA
authors.lastname: Wallace
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D004795:Enzyme Stability / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010649:Phenylalanine / Q000235:genetics; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D013050:Spectrometry, Fluorescence; D013448:Sulfobromophthalein / Q000378:metabolism; D014364:Tryptophan / Q000235:genetics
chemical_list: D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D007527:Isoenzymes; D008024:Ligands; D010446:Peptide Fragments; D013448:Sulfobromophthalein; D020106:Acrylamide; D010649:Phenylalanine; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha