title: Persistent CCR5 utilization and enhanced macrophage tropism by primary blood human immunodeficiency virus type 1 isolates from advanced stages of disease and comparison to tissue-derived isolates.
abstract: Viral phenotype, tropism, coreceptor usage, and envelope gene diversity were examined in blood isolates collected from 27 individuals at different stages of human immunodeficiency virus type 1 (HIV-1) disease and tissue derived isolates from 10 individuals with AIDS. The majority (89%) of blood and all tissue HIV-1 isolates from all stages of infection were non-syncytium inducing and macrophage (M) tropic. Tropism and productive infection by HIV isolates in both monocytes and monocyte-derived macrophages (MDM) increased in advanced disease (HIV tropism for monocytes, 1 of 6 from categories I and II versus 11 of 21 [P = 0.05] from category IV and II [CD4 < 250]; and high-level replication in MDM, 1 of 6 from categories I and II versus 16 of 21 from categories IV and II [P = 0. 015]). There was a high level of replication of blood and tissue isolates in T lymphocytes without restriction at any stage. Overall, the level of replication in MDM was 5- to 10-fold greater than in monocytes, with restriction in the latter occurring mainly at entry and later stages of replication. Only three blood isolates were identified as syncytium inducing, and all had a dualtropic phenotype. There was a significant increase of HIV envelope gene diversity, as shown by a heteroduplex mobility assay, in advanced disease; this may partly underlie the increase of HIV replication in MDM. Unlike blood isolates (even those from patients with advanced disease), tissue isolates displayed greater similarities (90%) in productive infection between MDM and monocytes. The majority (87%) of all isolates, including those from patients with advanced disease, used CCR5, and only 5 of 37 isolates showed expanded coreceptor usage. These results indicate that in the late stage of disease with increasing viral load and diversity, CCR5 utilization and M-tropism persist in blood and tissue and the replicative ability in macrophages increases. This suggests that these characteristics are advantageous to HIV and are important to disease progression.
authors.affiliation: Centre for Virus Research, Westmead Millennium Institute, National Centre for HIV Virology Research, Westmead, NSW 2145, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Juarez
authors.forename: M
authors.initials: M
authors.lastname: Alali
authors.forename: D
authors.initials: D
authors.lastname: Dwyer
authors.forename: R
authors.initials: R
authors.lastname: Collman
authors.forename: A
authors.initials: A
authors.lastname: Cunningham
authors.forename: H M
authors.initials: HM
authors.lastname: Naif
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D000595:Amino Acid Sequence; D018450:Disease Progression; D005838:Genotype; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism; D015658:HIV Infections / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000821:virology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000821:virology; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D019713:Receptors, CCR5 / Q000378:metabolism*; D015728:Receptors, HIV; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D018523:Tropism; D014779:Virus Replication
chemical_list: D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D019713:Receptors, CCR5; D015728:Receptors, HIV