title: Biodistribution of 99Tcm-labelled 5-thio-D-glucose.
abstract: We studied the biodistribution and tumour localization of 99Tcm-labelled-5-thio-D-glucose (99Tcm-TG). 5-Thio-D-glucose was labelled with 99Tcm by direct stannous ion reduction. The biodistribution of 99Tcm-TG was investigated in normal rabbits and in mice bearing experimental tumours. In rabbits, the plasma and clearance of 99Tcm-TG was 14.5 +/- 2.0 and 11.3 +/- 3.0 ml.min-1 respectively. Urinary excretion at 1 h was 53 +/- 5%. 99Tcm-TG was injected intravenously in mice bearing MC26 colon carcinoma and tissue samples were analysed by gamma scintillation counting at various times. Uptake of 99Tcm-TG in tumour at 1 and 3 h was 1.6 +/- 0.3% and 1.2 +/- 0.3%; the tumour to muscle ratios were 2.7:1 and 4:1 respectively. The autoradiographic biodistribution of 99Tcm-TG in MX-1 human breast xenografted nude mice showed more persistent tumour uptake of 99Tcm-TG than 14C-2-deoxyglucose (14C-DG). 99Tcm-TG accumulated in the centre of the tumours; 14C-DG was decreased in this central region probably because of zones of infarction on necrosis. The discordance between the tumour uptake of 99Tcm-TG and 14C-DG indicates that 99Tcm-TG does not act like a glucose analog, suggesting 99Tcm-TG avidity for zones of infarction or necrosis. The further study of 99Tcm-TG in tumours and ischaemic injury is warranted.
authors.affiliation: Division of Nuclear Medicine, Medical College of Wisconsin, Milwaukee 53226, USA. ozker@mcw.edu
authors.forename: K
authors.initials: K
authors.lastname: Ozker
authors.forename: B D
authors.initials: BD
authors.lastname: Collier
authors.forename: D J
authors.initials: DJ
authors.lastname: Lindner
authors.forename: L
authors.initials: L
authors.lastname: Kabasakal
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: A Z
authors.initials: AZ
authors.lastname: Krasnow
authors.forename: R S
authors.initials: RS
authors.lastname: Hellman
authors.forename: S D
authors.initials: SD
authors.lastname: Edwards
authors.forename: C R
authors.initials: CR
authors.lastname: Bourque
authors.forename: P D
authors.initials: PD
authors.lastname: Crane
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001345:Autoradiography; D001943:Breast Neoplasms / Q000000981:diagnostic imaging; D003847:Deoxyglucose; D005947:Glucose / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000000981:diagnostic imaging; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
chemical_list: D000963:Antimetabolites; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C402965:Tc-99m-thio-D-glucose; D003847:Deoxyglucose; D005947:Glucose