Epileptiform activity induced by 4-aminopyridine in entorhinal cortex hippocampal slices of rats with a genetically determined absence epilepsy (GAERS).
Patients with absence epilepsy frequently develop convulsions later in life. We were therefore interested whether tissue from rats with a genetic absence epilepsy is more prone to seizure generation than normal animals. We compared the epileptiform activities induced by 4-aminopyridine (4-AP) induced in hippocampal-entorhinal cortex slices from genetic absence epilepsy rats of Strasbourg (GAERS, age 6 months) in which absence seizures have been present for about 4 months and from control non epileptic rats (NE). 4-AP induced short recurrent discharges in area CA1 of rat hippocampus, seizure-like events and interictal discharges in the entorhinal cortex. The various epileptiform discharges did not differ between the two strains in amplitude, duration and frequency. However, the latency for induction of different epileptiform activities by 50 microM 4-AP was significantly shorter in GAERS (about 16 min) than in NE rats (about 25 min). We also analysed differences in evoked field potentials (fp) in hippocampal area CA1 before, during and after application of 4-AP. Before application of 4-AP, responses to stimulation of Schaffer collateral were smaller in GAERS than in NE rats. Paired pulse potentiation was significantly larger in GAERS than in NE rats. 4-AP in the bath augmented the size of the evoked field potentials and this increase was larger in GAERS than in NE rats. Our findings show a greater excitability of hippocampal area CA1 in GAERS rats and a greater ability to develop 4-AP-induced epileptiform activity in combined hippocampal-enthorhinal cortex slices in GAERS than in NE rats.
Armand
V
V
Department of Neurophysiology, Institute of Physiology of the Charité, Humboldt University Berlin, Germany. varmand@broca.inserm.fr
Hoffmann
P
P
Vergnes
M
M
Heinemann
U
U
D015761:4-Aminopyridine / Q000494:pharmacology*; D000818:Animals; D018728:Entorhinal Cortex / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D004832:Epilepsy, Absence / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology*; D005071:Evoked Potentials / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011922:Rats, Mutant Strains; D012016:Reference Values; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology
D015761:4-Aminopyridine