Inducible nitric oxide synthase protection against coxsackievirus pancreatitis.
Coxsackievirus infection causes myocarditis and pancreatitis in humans. In certain strains of mice, Coxsackievirus causes a severe pancreatitis. We explored the role of NO in the host immune response to viral pancreatitis. Coxsackievirus replicates to higher titers in mice lacking NO synthase 2 (NOS2) than in wild-type mice, with particularly high viral titers and viral RNA levels in the pancreas. Mice lacking NOS have a severe, necrotizing pancreatitis, with elevated pancreatic enzymes in the blood and necrotic acinar cells. Lack of NOS2 leads to a rapid increase in the mortality of infected mice. Thus, NOS2 is a critical component in the immune response to Coxsackievirus infection.
Zaragoza
C
C
Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Ocampo
C J
CJ
Saura
M
M
Bao
C
C
Leppo
M
M
Lafond-Walker
A
A
Thiemann
D R
DR
Hruban
R
R
Lowenstein
C J
CJ
D000208:Acute Disease; D000818:Animals; D003384:Coxsackievirus Infections / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000517:prevention & control*; D018792:Encephalitis, Viral / Q000209:etiology; D029822:Enterovirus B, Human; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009205:Myocarditis / Q000209:etiology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D052247:Nitric Oxide Synthase Type II; D010179:Pancreas / Q000201:enzymology / Q000821:virology; D010195:Pancreatitis / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000517:prevention & control*
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse