title: Gastroesophageal reflux after combined lower esophageal sphincter and diaphragmatic crural sling inactivation in the rat.
abstract: This study tests the hypothesis that either selective or combined destruction of the lower esophageal sphincter and the diaphragmatic crural sling should induce reflux in the rat. Pull-through perfusion manometry was performed before and after lower esophageal myectomy, crural myotomy, or both. pH monitoring was used to detect reflux. Unmanipulated rats served as controls. Paired t tests were used for comparison of pre- and postoperative pressure values and contingency tables with Fisher's tests for examining the association between the interventions and the appearance of reflux. Esophageal myectomy decreased only sphincteric pressure from 25.9+/-15.5 to 9+/-6 mm Hg (P < 0.01), whereas crural myotomy decreased only sling pressure from 26.2+/-13.3 to 7.3+/-3.9 mm Hg (P < 0.01). Simultaneous performance of both procedures decreased sphincteric and crural pressures from 20.4+/-7.5 to 7.6+/-4.3 mm Hg (P < 0.01) and from 45.9+/-20.6 to 18.2+/-7.4 mm Hg (P < 0.01), respectively. None of the control, myectomy, or myotomy animals showed reflux upon pH-metry but 5/8 rats in which both procedures were performed had prolonged acid exposure. No esophagitis was seen. In conclusion, normal rats do not have reflux. Selective destruction of either the sphincter or the crural sling does not induce reflux, despite causing flattening of their respective manometric profiles. Conversely, combined inactivation of both components is significantly associated with reflux.
authors.affiliation: Department of Pediatric Surgery, Hospital Infantil Universitario La Paz, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Montedonico
authors.forename: J A
authors.initials: JA
authors.lastname: Diez-Pardo
authors.forename: J A
authors.initials: JA
authors.lastname: Tovar
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000502:physiology / Q000601:surgery*; D004943:Esophagogastric Junction / Q000502:physiology / Q000601:surgery*; D005764:Gastroesophageal Reflux / Q000209:etiology* / Q000517:prevention & control; D006863:Hydrogen-Ion Concentration; D008297:Male; D008365:Manometry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar