title: Evidence against a role of physiological concentrations of estrogen in post-myocardial infarction remodeling.
abstract: OBJECTIVE
  The purpose of this study was to examine whether endogenous estrogen deficiency induced by ovariectomy affects chronic left ventricular dysfunction post-myocardial infarction (MI).
  
  
  BACKGROUND
  Epidemiologic findings suggest that mortality of postmenopausal women is increased after MI, but the underlying mechanisms are unknown.
  
  
  METHODS
  Rats were either not ovariectomized (non-OVX), ovariectomized (OVX) or ovariectomized and treated with subcutaneous 17-beta-estradiol (E2) pellets (OVX + E2). Two weeks later, animals were sham-operated (Sham) or left coronary artery ligated (MI). Eight weeks later, in vivo echocardiographic and hemodynamic measurements were performed. Thereafter, hearts were isolated and perfused isovolumically.
  
  
  RESULTS
  Mean infarct size was similar among the three MI groups. Ovariectomy decreased serum E2 levels (11 +/- 4 vs. 49 +/- 11 pg/ml in non-OVX, p < 0.01) and increased body weight. These changes were reversed by E2 replacement. The degree of cardiac hypertrophy was similar for all groups post-MI. Left ventricular diameters were increased post-MI (8.9 +/- 0.4 in non-OVX + MI vs. 6.7 +/- 0.2 mm in non-OVX + Sham hearts, p < 0.0001), but OVX or OVX + E2 replacement did not alter left ventricular diameters in post-MI and Sham hearts. Left ventricular fractional shortening was severely impaired post-MI (19 +/- 2% vs. 50 +/- 3 in non-OVX + Sham hearts, p < 0.0001) with no influence of hormonal status. Left ventricular end-diastolic pressure, measured in vivo, was increased in all MI groups without significant differences between groups. Pressure-volume curves, obtained in perfused hearts, demonstrated a right and downward shift with reduced maximum left ventricular developed pressure post-MI (75 +/- 6 vs. 108 +/- 3 mm Hg in non-OVX + Sham hearts, p < 0.001) and were also unaffected by either OVX or E2 replacement.
  
  
  CONCLUSIONS
  Chronic endogenous estrogen deficiency does not have major effects on the development of cardiac hypertrophy, dysfunction and dilation post-MI.
authors.affiliation: Medizinische Universitätsklinik, Universität Würzburg, Germany. huegel@mail.uni-wuerzburg.de
authors.forename: S
authors.initials: S
authors.lastname: Hügel
authors.forename: M
authors.initials: M
authors.lastname: Reincke
authors.forename: H
authors.initials: H
authors.lastname: Strömer
authors.forename: J
authors.initials: J
authors.lastname: Winning
authors.forename: M
authors.initials: M
authors.lastname: Horn
authors.forename: C
authors.initials: C
authors.lastname: Dienesch
authors.forename: P
authors.initials: P
authors.lastname: Mora
authors.forename: H H
authors.initials: HH
authors.lastname: Schmidt
authors.forename: B
authors.initials: B
authors.lastname: Allolio
authors.forename: S
authors.initials: S
authors.lastname: Neubauer
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015150:Echocardiography, Doppler; D004967:Estrogens / Q000502:physiology*; D005069:Evaluation Studies as Topic; D005260:Female; D006439:Hemodynamics; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: D004967:Estrogens