Isolation of a new ribonuclease from fresh fruiting bodies of the straw mushroom.
Although ribonucleases have been isolated from numerous organisms in the animal and plant kingdoms, only a few have been reported from mushrooms. In this investigation, a novel ribonuclease, with an N-terminal amino acid sequence distinctly different from those of the previously published mushroom ribonucleases, was isolated from the fruiting bodies of the straw mushroom. It exhibited a molecular weight of 42.5 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis which was larger than those of ribonucleases from other organisms. The isolation procedure entailed extraction with aqueous buffer, (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-Sepharose CL6B, and gel filtration on Superdex 75 using fast protein liquid chromatography. Straw mushroom ribonuclease was relatively specific for polyU and hydrolyzed yeast tRNA with a pH optimum between 6.5 and 7.5.
Wang
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Department of Microbiology, China Agricultural University, Beijing, China.
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D000595:Amino Acid Sequence; D002845:Chromatography; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D005656:Fungal Proteins; D012260:Ribonucleases