title: Temporal mapping of transcripts in human herpesvirus-7.
abstract: Transcription of human herpesvirus-7 (HHV-7) in cultures of productively infected T-cells was studied. Transcription of HHV-7 was regulated by the typical herpesvirus cascade in which alpha, beta and gamma genes are sequentially transcribed. Transcripts of U10, U14, U18, U31, U39, U41, U42, U53, U73 and U89/90 were detected 3 h after infection and were not inhibited by the absence of protein synthesis and therefore were alpha functions. U19 and U18/20 were beta genes; their transcription was inhibited by cycloheximide but not by phosphonoacetate, an inhibitor of DNA synthesis. U60/66 and U98/100 were gamma genes since their spliced transcripts were not detected in cells treated with phosphonoacetate. HHV-7 transcription was regulated by complex mechanisms, which involve the temporal coordinated activation of specific viral promoters and post-transcriptional processing. Splice mechanisms were also temporally regulated. Transcription of U89/90 pre-mRNA and splice took place simultaneously in the immediate-early phase. On the other hand, U16/17 pre-mRNA was synthesized with typical alpha kinetics, but the spliced product was regulated as a beta function. Likewise, the primary transcripts of U60/66 and U98/100 were alpha and beta, respectively, but both spliced products were synthesized in the late phase of virus replication. Finally, HHV-7 supported a bona fide latent infection in the adult population, since viral transcripts were not detected in peripheral blood mononuclear cells of healthy donors infected with HHV-7.
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Paola
authors.initials: P
authors.lastname: Menegazzi
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Monica
authors.initials: M
authors.lastname: Galvan
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Antonella
authors.initials: A
authors.lastname: Rotola
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Tullia
authors.initials: T
authors.lastname: Ravaioli
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Arianna
authors.initials: A
authors.lastname: Gonelli
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Enzo
authors.initials: E
authors.lastname: Cassai
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy1.
authors.forename: Dario
authors.initials: D
authors.lastname: Di Luca
mesh_terms: D002874:Chromosome Mapping; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005814:Genes, Viral; D016199:Herpesvirus 7, Human / Q000235:genetics*; D006801:Humans; D012326:RNA Splicing; D012367:RNA, Viral; D013601:T-Lymphocytes / Q000821:virology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D017735:Virus Latency
chemical_list: D012367:RNA, Viral