Development of monoclonal ELISAs for azinphos-methyl. 1. Hapten synthesis and antibody production.
The development of monoclonal antibody-based enzyme-linked immunosorbent assays for azinphos-methyl is described. A panel of haptens was synthesized for immunoconjugate preparation, and a series of haptens for heterologous, coating or tracer, conjugates was also prepared. Hapten synthesis was based on a strategy in which only a fragment of the whole target molecule was present (fragmentary haptens). From immunized mice, a set of monoclonal antibodies was obtained and ELISA sensitivities were assayed in different formats. Affinities estimated as I(50) values in the low nanomolar range for azinphos-methyl and phosmet were observed for several monoclonal antibodies in the conjugate-coated format and in the antibody-coated format under nonoptimized assay conditions.
Mercader
J V
JV
Laboratori Integrat de Bioenginyeria, Universitat Politècnica de València, Camí de Vera s/n, E-46022 València, Spain.
Montoya
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001387:Azinphosmethyl / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens; D007306:Insecticides / Q000032:analysis; D051379:Mice; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006241:Haptens; D007306:Insecticides; D001387:Azinphosmethyl