D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose.
Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Transcription of the two allose operons, measured as beta-galactosidase activity specified by alsI-lacZ(+) or alsE-lacZ(+) operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.
Poulsen
T S
TS
Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark.
Chang
Y Y
YY
Hove-Jensen
B
B
D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism*; D007535:Isomerases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D019998:Racemases and Epimerases / Q000235:genetics; D018085:Regulon; D012266:Ribose / Q000378:metabolism*; D014158:Transcription, Genetic
C002055:allose; D012266:Ribose; D007535:Isomerases; D019998:Racemases and Epimerases; D005947:Glucose