Growth and cytotoxic activity by Mycobacterium ulcerans in protein-free media.
The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein-free growth conditions.
Mve-Obiang
A
A
Laboratoire de Biochimie et Biologie Cellulaire, Facultés Universitaires Notre Dame de la Paix, Rue de Bruxelles 61, B-5000, Namur, Belgium.
Remacle
J
J
Palomino
J C
JC
Houbion
A
A
Portaels
F
F
D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology; D019911:Mycobacterium ulcerans / Q000254:growth & development* / Q000378:metabolism*
D001427:Bacterial Toxins; D003470:Culture Media