title: Effect of trilostane on PGE, PGF2alpha, estradiol-17beta, and progesterone secretion and pregnancy of 90-day ovariectomized pregnant ewes.
abstract: Ninety-day pregnant sheep were ovariectomized and received vehicle or trilostane every 12 h through 132 h, starting at 72 h postovariectomy. All trilostane-treated ewes aborted (P < or = 0.05) between 36 and 50 h after initiation of treatment. Profiles of progesterone in jugular venous blood differed (P < or = 0.05) and was lower (P < or = 0.05) in trilostane-treated ewes. Profiles of estradiol-17beta in jugular venous plasma of trilostane-treated ewes differed (P < or = 0.05) from controls. Estradiol-17beta increased after the first two treatments, followed by a return 2 h later to pretreatment levels (P > or = 0.05), which was followed by a sustained increase (P < or = 0.05) in estradiol-17beta. Profiles of PGF2alpha in inferior vena cava plasma of trilostane-treated ewes differed and were greater (P < or = 0.05) and occurred with the sustained increase in estradiol-17beta and the onset of most of the abortions. Profiles of PGE in inferior vena cava plasma between control and trilostane-treated 90-day pregnant ewes did not differ (P > or = 0.05). It is concluded that abortions occur at midpregnancy in sheep when the estradiol-17beta : progesterone ratio changes sufficiently to cause a sustained increase in estradiol-17beta and PGF2alpha but without changing placental secretion of PGE.
authors.affiliation: Dept. of Animal Science, University of Hawaii, Honolulu 96822, USA.
authors.forename: Y S
authors.initials: YS
authors.lastname: Weems
authors.forename: P J
authors.initials: PJ
authors.lastname: Bridges
authors.forename: B R
authors.initials: BR
authors.lastname: LeaMaster
authors.forename: D L
authors.initials: DL
authors.lastname: Vincent
authors.forename: C W
authors.initials: CW
authors.lastname: Weems
mesh_terms: D000021:Abortifacient Agents, Steroidal / Q000494:pharmacology*; D000034:Abortion, Veterinary; D000818:Animals; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D015237:Dinoprost / Q000097:blood*; D005260:Female; D010052:Ovariectomy; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D011374:Progesterone / Q000097:blood*; D011458:Prostaglandins E / Q000097:blood*; D012756:Sheep / Q000502:physiology*; D013997:Time Factors
chemical_list: D000021:Abortifacient Agents, Steroidal; D011458:Prostaglandins E; D013196:Dihydrotestosterone; D011374:Progesterone; D015237:Dinoprost; C009954:trilostane