title: Analysis of Epstein-Barr virus (EBV) nuclear antigen 1 subtypes in EBV-associated lymphomas from Brazil and the United Kingdom.
abstract: EBNA-1 is the only viral protein consistently expressed in all cells latently infected by Epstein-Barr virus (EBV). There is a high frequency of sequence variation within functionally important domains of EBNA-1, with five subtypes identified. Individuals may be infected with multiple EBV strains (classified according to EBNA-1 subtype), but Burkitt's lymphoma (BL) tumours carry a single subtype and exhibit some subtype preference. Subtype variation has also been related to geographical location. In the present study EBNA-1 polymorphisms were examined in a series of haematological malignancies from two distinct geographical regions, Brazil and the United Kingdom. Nucleotide sequence analysis of the carboxy-terminal region of EBNA-1 in 34 cases revealed six distinct sequences, some of which are novel. A new subtype, named V-Ala, was identified. EBNA-1 subtype in tumours differed markedly according to geographical location. In contrast to previous studies, we found evidence of EBNA-1 sequence variation within individual BL tumour samples.
authors.affiliation: LRF Virus Centre, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK1.
authors.forename: Jane
authors.initials: J
authors.lastname: MacKenzie
authors.affiliation: LRF Virus Centre, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK1.
authors.forename: Diane
authors.initials: D
authors.lastname: Gray
authors.affiliation: Department of Pathology, Santa Casa de São Paulo, São Paulo, CEP 01277, Brazil2.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pinto-Paes
authors.affiliation: Department of Pathology, Santa Casa de São Paulo, São Paulo, CEP 01277, Brazil2.
authors.forename: Luis F M
authors.initials: LFM
authors.lastname: Barrezueta
authors.affiliation: LRF Virus Centre, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK1.
authors.forename: Alison A
authors.initials: AA
authors.lastname: Armstrong
authors.affiliation: Department of Public Health Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh EH8 9AG, UK 3.
authors.forename: Freda A
authors.initials: FA
authors.lastname: Alexander
authors.affiliation: MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, UK4.
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: McGeoch
authors.affiliation: LRF Virus Centre, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK1.
authors.forename: Ruth F
authors.initials: RF
authors.lastname: Jarrett
mesh_terms: D001483:Base Sequence; D001938:Brazil; D002051:Burkitt Lymphoma / Q000821:virology*; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D006113:United Kingdom
chemical_list: D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens