Differential responses of respiratory nuclei to anoxia in rhythmic brain stem slices of mice.
The response of the neonatal respiratory system to hypoxia is characterized by an initial increase in ventilation, which is followed within a few minutes by a depression of ventilation below baseline levels. We used the transverse medullary slice of newborn mice as a model system for central respiratory control to investigate the effects of short-lasting periods of anoxia. Extracellular population activity was simultaneously recorded from the ventral respiratory group (VRG) and the hypoglossus (XII) nucleus (a respiration-related motor output nucleus). During anoxia, respiratory frequency was modulated in a biphasic manner and phase-locked in both the VRG and the XII. The amplitude of phasic respiratory bursts was increased only in the XII and not in the VRG. This increase in XII burst amplitude commenced approximately 1 min after the anoxic onset concomitant with a transient increase in tonic activity. The burst amplitude remained elevated throughout the entire 5 min of anoxia. Inspiratory burst amplitude in the VRG, in contrary, remained constant or even decreased during anoxia. These findings represent the first simultaneous extracellular cell population recordings of two respiratory nuclei. They provide important data indicating that rhythm generation is altered in the VRG without a concomitant alteration in the VRG burst amplitude, whereas the burst amplitude is modulated only in the XII nucleus. This has important implications because it suggests that rhythm generation and motor pattern generation are regulated separately within the respiratory network.
Telgkamp
P
P
Department of Organismal Biology and Anatomy, The University of Chicago, Chicago, Illinois 60637, USA.
Ramirez
J M
JM
D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000502:physiology* / Q000503:physiopathology; D005260:Female; D007002:Hypoglossal Nerve / Q000502:physiology / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D001239:Inhalation / Q000502:physiology; D008297:Male; D051379:Mice; D009474:Neurons / Q000502:physiology*; D015656:Respiratory Mechanics / Q000502:physiology*