title: Modification of bovine beta-lactoglobulin by glycation in a powdered state or in an aqueous solution: immunochemical characterization.
abstract: Bovine beta-LG was modified by glycation with lactose in a powdered state or in an aqueous solution. An immunological characterization was performed using monoclonal antibodies with defined epitopes. The results showed that the structural changes were confined to the AB loop region of the molecules when glycation was conducted in a restricted water environment and had little consequences on the association state of glycated beta-LG. The protein conformation was much more extensively modified when glycation was performed in an aqueous solution at 60 degrees C, despite a lower glycation extent. These structural changes were located at the dimer interface (AB loop, GH loop, beta-strand I, and alpha-helix). These results allowed us to establish a relationship between the conformational changes and the modification of the association state of the glycated protein (formation of disulfide bridges between the free thiol groups of two monomers), previously described.
authors.affiliation: INRA-LRTL, Biochemistry Unit, 65 rue de Saint Brieuc, F-35042 Rennes Cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Morgan
authors.forename: A
authors.initials: A
authors.lastname: Vénien
authors.forename: S
authors.initials: S
authors.lastname: Bouhallab
authors.forename: D
authors.initials: D
authors.lastname: Mollé
authors.forename: J
authors.initials: J
authors.lastname: Léonil
authors.forename: G
authors.initials: G
authors.lastname: Peltre
authors.forename: D
authors.initials: D
authors.lastname: Levieux
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D018360:Crystallography, X-Ray; D006031:Glycosylation; D007782:Lactoglobulins / Q000378:metabolism*; D008892:Milk; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012996:Solutions
chemical_list: D000911:Antibodies, Monoclonal; D007782:Lactoglobulins; D012996:Solutions