title: High-density small-volume gel loading directly from capillary tubes.
abstract: A technique has been developed for high lane density loading of small-volume DNA samples in a horizontal agarose gel. This technique has been investigated with a simple hand-held tool that is made to couple to sample output from a new capillary-based sample automation system. The approach consists of piercing the gel with pressurized sample capillaries and relieving the pressure shortly before withdrawal. The pressurization prevents the capillary from aspirating the gel buffer and keeps the sample at the tip of the capillary, so that it may be sucked into the gel during withdrawal. This method is shown to be adequate for a wide range of DNA ladders and PCR-based screening. In addition to allowing smaller lanes and a higher lane density than is achievable with traditional well-forming techniques, it relaxes the need for well formation and the alignment of the sample loader with those wells, providing an easy, efficient means of loading agarose gels.
authors.affiliation: University of Washington, Seattle 98195-2500, USA.
authors.forename: H T
authors.initials: HT
authors.lastname: Evensen
authors.forename: D R
authors.initials: DR
authors.lastname: Meldrum
authors.forename: C
authors.initials: C
authors.lastname: Saenphimmachak
authors.forename: E E
authors.initials: EE
authors.lastname: Dixon
mesh_terms: D000990:Antithrombin III / Q000235:genetics; D001331:Automation; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D000990:Antithrombin III; D004247:DNA