Identification of oxidative stress-regulated genes in rat aortic smooth muscle cells by suppression subtractive hybridization.
A suppression subtractive hybridization technique was used to identify reactive oxygen species (ROS)-regulated genes in rat vascular smooth muscle cells. Three genes out of 89 clones, identified as fibronectin, p105 coactivator and ECA39, showed increased expression after treatment with H(2)O(2). The mRNA expressions of these three genes were induced in a time- and dose-dependent manner, independent of protein kinase C activation. Immunohistochemical staining showed that the p105 coactivator expression was markedly induced in the neointima of balloon-injured rat carotid arteries. These results suggest that ROS may play an important role in the development of atherosclerosis by regulating the gene expressions we identified in this study.
Sakamoto
K
K
Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan.
Yamasaki
Y
Y
Kaneto
H
H
Fujitani
Y
Y
Matsuoka
T
T
Yoshioka
R
R
Tagawa
T
T
Matsuhisa
M
M
Kajimoto
Y
Y
Hori
M
M
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D015152:Blotting, Northern; D002339:Carotid Arteries / Q000378:metabolism*; D002478:Cells, Cultured; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D018384:Oxidative Stress / Q000235:genetics*; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors; D000637:Transaminases
D005353:Fibronectins; D009124:Muscle Proteins; D011506:Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; C490589:Bcat1 protein, rat; D000637:Transaminases