title: Cathepsin S controls the trafficking and maturation of MHC class II molecules in dendritic cells.
abstract: Before a class II molecule can be loaded with antigenic material and reach the surface to engage CD4+ T cells, its chaperone, the class II-associated invariant chain (Ii), is degraded in a stepwise fashion by proteases in endocytic compartments. We have dissected the role of cathepsin S (CatS) in the trafficking and maturation of class II molecules by combining the use of dendritic cells (DC) from CatS(-/-) mice with a new active site-directed probe for direct visualization of active CatS. Our data demonstrate that CatS is active along the entire endocytic route, and that cleavage of the lysosomal sorting signal of Ii by CatS can occur there in mature DC. Genetic disruption of CatS dramatically reduces the flow of class II molecules to the cell surface. In CatS(-/-) DC, the bulk of major histocompatibility complex (MHC) class II molecules is retained in late endocytic compartments, although paradoxically, surface expression of class II is largely unaffected. The greatly diminished but continuous flow of class II molecules to the cell surface, in conjunction with their long half-life, can account for the latter observation. We conclude that in DC, CatS is a major determinant in the regulation of intracellular trafficking of MHC class II molecules.
authors.affiliation: Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: C
authors.initials: C
authors.lastname: Driessen
authors.forename: R A
authors.initials: RA
authors.lastname: Bryant
authors.forename: A M
authors.initials: AM
authors.lastname: Lennon-Duménil
authors.forename: J A
authors.initials: JA
authors.lastname: Villadangos
authors.forename: P W
authors.initials: PW
authors.lastname: Bryant
authors.forename: G P
authors.initials: GP
authors.lastname: Shi
authors.forename: H A
authors.initials: HA
authors.lastname: Chapman
authors.forename: H L
authors.initials: HL
authors.lastname: Ploegh
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology* / Q000648:ultrastructure; D004705:Endocytosis; D005434:Flow Cytometry; D005805:Genes, MHC Class I; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D008024:Ligands; D008565:Membrane Proteins / Q000494:pharmacology / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology; D013347:Subcellular Fractions / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; D008024:Ligands; D008565:Membrane Proteins; C084881:flt3 ligand protein; D002403:Cathepsins; C028217:cathepsin S