title: Brain-derived neurotropic factor prevents superoxide anion-induced death of PC12h cells stably expressing TrkB receptor via modulation of reactive oxygen species.
abstract: In our previous report (Satoh et al., 1999. Regulation of reactive oxygen species by nerve growth factor but not by Bcl-2 as a novel mechanism of protection of PC12 cells from superoxide anion-induced death. J. Biochem. 125, 952-959), we reported that nerve growth factor (NGF) protected PC12 cells from superoxide anion (O2-)-induced cell death through a novel regulation of reactive oxygen species (ROS) which increased O2- and decreased hydrogen peroxide (H2O2), indicating that decreasing conversion from O2- to H2O2 is a critical process for the protection by NGF. In the present study, we performed a comparative study on protective mechanisms between NGF and brain-derived neurotrophic factor (BDNF) using TrkB-expressing PC12h cells. When compared with NGF, BDNF induced a weaker but significant protective effect on the cells from O2- induced death. BDNF did not seem to change the total amount of ROS in the cells treated with xanthine and xanthine oxidase. On the other hand, BDNF increased O2- and decreased H2O2- levels in the same cells, although not so strongly as NGF. These results suggest that decreasing conversion from O2- to H2O2 is also critical for the protection by BDNF, which is considered to play a central role in survival and differentiation of CNS neurons.
authors.affiliation: Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Suita, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamagata
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: A
authors.initials: A
authors.lastname: Nakatani
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Ikeuchi
authors.forename: H
authors.initials: H
authors.lastname: Hatanaka
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009414:Nerve Growth Factors / Q000494:pharmacology / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000502:physiology*; D020813:Receptor, trkB / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014162:Transfection; D019820:Xanthine / Q000633:toxicity; D014969:Xanthine Oxidase / Q000633:toxicity
chemical_list: C081722:2.5S nerve growth factor; D019208:Brain-Derived Neurotrophic Factor; D005456:Fluorescent Dyes; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D013481:Superoxides; D019820:Xanthine; D014969:Xanthine Oxidase; D020813:Receptor, trkB