Conopeptides from Conus striatus and Conus textile by cDNA cloning.
Conopeptide content in Conus textile and Conus striatus venoms were examined by polymerase chain reaction amplification of alpha-conopeptide cDNA and rapid amplification of 3' cDNA ends of O-superfamily conopeptide cDNA. Two new alpha-conopeptide sequences and six new O-superfamily conopeptide sequences from C. textile, four new O-superfamily conopeptide sequences, and four previously biochemically characterized conopeptide sequences from C. striatus were identified. The results suggest that this cDNA method is rapid and requires less material for the study of conopeptides.
Lu
B S
BS
Institute of Biotechnology, Beijing, China. lbszy@public.east.cn.net
Yu
F
F
Zhao
D
D
Huang
P T
PT
Huang
C F
CF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020916:Conotoxins / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012908:Snails / Q000235:genetics; D020960:omega-Conotoxins / Q000235:genetics*
D020916:Conotoxins; D018076:DNA, Complementary; D010455:Peptides; C534777:SO3 conotoxin, Conus striatus; D020960:omega-Conotoxins