title: Determination of the wax ester content in olive oils. Improvement in the method proposed by EEC regulation 183/93.
abstract: A simpler and faster procedure than the official one described in document IV of European Economic Community Regulation 183/93 is proposed. The wax ester fraction is isolated from triglycerides using a commercially available silica gel column and carbon tetrachloride as eluent. The recovered wax ester fraction, with the addition of a suitable internal standard solution, is analyzed by gas chromatography. A column with a 65% phenyl methyl silicone stationary phase allows a satisfying separation of wax ester fraction in comparison with both a steryl ester and a light fraction eluted before the internal standard. Furthermore, also the single components of the wax ester fraction are suitably separated.
authors.affiliation: Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici, Napoli, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Nota
authors.forename: D
authors.initials: D
authors.lastname: Naviglio
authors.forename: R
authors.initials: R
authors.lastname: Romano
authors.forename: V
authors.initials: V
authors.lastname: Sabia
authors.forename: S S
authors.initials: SS
authors.lastname: Musso
authors.forename: C
authors.initials: C
authors.lastname: Improta
mesh_terms: D002849:Chromatography, Gas; D004952:Esters; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D004952:Esters; D000069463:Olive Oil; D010938:Plant Oils