Tissue-inherent fate of GPI revealed by GPI-anchored GFP transgenesis.
To clarify the fate of glycosylphosphatidylinositol (GPI) in mammals, we developed GPI-anchored enhanced green fluorescent protein (EGFP-GPI) and transgenic mice carrying this fusion construct. When it was introduced to culture cells, the EGFP-GPI protein was correctly sorted to plasma membranes and microsomes depending on GPI biosynthesis. Transgenic mice carrying EGFP-GPI were found to show a broad transgene expression. Histologically, a prominent polarized localization of EGFP-GPI protein was observed in various epithelia, the nervous system and liver and secreted from some exocrine glands, as well as non-polarized presence in non-epithelial tissues, demonstrating a tissue-inherent manner of GPI sorting.
Kondoh
G
G
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, Suita, Japan. kondohg@mr-envi.med.osaka-u.ac.jp
Gao
X H
XH
Nakano
Y
Y
Koike
H
H
Yamada
S
S
Okabe
M
M
Takeda
J
J
D000176:Acrosin / Q000737:chemistry / Q000235:genetics; D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D017261:Glycosylphosphatidylinositols / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006651:Histocytochemistry; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000235:genetics; D018800:Thy-1 Antigens / Q000737:chemistry / Q000235:genetics; D014162:Transfection
D017261:Glycosylphosphatidylinositols; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D018800:Thy-1 Antigens; D049452:Green Fluorescent Proteins; D000176:Acrosin