A microfabricated fluorescence-activated cell sorter.
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (microFACS) for sorting various biological entities. Compared with conventional FACS machines, the microFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used microFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
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Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.
Spence
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Scherer
A
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Arnold
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FH
Quake
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SR
D004926:Escherichia coli / Q000166:cytology; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D012828:Silicones
D008164:Luminescent Proteins; D012828:Silicones; D049452:Green Fluorescent Proteins