Reentrant tachyarrhythmias in right atria of cardiomyopathic versus healthy Syrian hamster.
We studied the role of acetylcholine (ACh) and calcium overload in the induction of atrial flutter or atrial fibrillation (AF) in right atria from 34 normal male Syrian hamsters (F1B) and 33 cardiomyopathic Syrian hamsters (BIO 14.6) associated with focal myocardial necrosis. Action potential (AP) was recorded with conventional microelectrode techniques and twitch force by a transducer. ACh (0.1, 1 and 10 microM) induced high-frequency AF (around 33 Hz) along with tension oscillations and contracture in 7 of 12 normal hamster atria. These effects of ACh were abolished by tetrodotoxin or quinidine as well as by atropine. In contrast, ACh induced AF only in 1 of 12 myopathic atria. In both normal and myopathic atria, ACh induced similar changes in AP duration, spontaneous rate and force. The effects of calcium overload were tested by means of a high [Ca(2+)](o) (8.1 mM) low [K(+)](o) (1 mM) solution in another series of experiments. This solution also induced incidence of AF higher in normal (10/12) than in myopathic atria (4/12). The calcium load was also increased by high-frequency pacing (32 Hz for 3 or 30 s): AF occurred in normal atria (5/8), but not in myopathic atria (0/8). Measurement of the refractory period revealed a longer refractory period in myopathic than in control atria. We concluded that the lower incidence of AF in myopathic atria was probably due to their longer refractory period and the associated focal myocardial necrosis which then hindered the establishment of such a reentrant rhythm.
Lin
C I
CI
Institute of Pharmacology, National Defense Medical Center, Taipei, Taiwan, ROC. bme03@ndmcl.ndmctsgh.edu.tw
Yiu
M Y
MY
Hwang
H R
HR
Lin
C L
CL
Chen
K Y
KY
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D016275:Atrial Function; D002118:Calcium / Q000378:metabolism; D009202:Cardiomyopathies / Q000503:physiopathology*; D006224:Cricetinae; D004558:Electric Stimulation; D006325:Heart Atria / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D011188:Potassium / Q000378:metabolism; D013610:Tachycardia / Q000503:physiopathology*
D000109:Acetylcholine; D011188:Potassium; D002118:Calcium