title: Use of the Actinobacillus actinomycetemcomitans leukotoxin promoter to drive expression of the green fluorescent protein in an oral pathogen.
abstract: The gene for the green fluorescent protein, gfp, was cloned, under the control of the Actinobacillus actinomycetemcomitans leukotoxin (ltx) promoter, in the A. actinomycetemcomitans shuttle vector, pSU20. A actinomycetemcomitans containing the ltx-gfp construct emitted bright green fluorescence in the standard invasion assay using epifluorescence microscopy. These data demonstrate that the green fluorescent protein will be a useful tool for the live analysis of A. actinomycetemcomitans interactions with host cells, and that the ltx promoter can be used to drive the expression of non-A. actinomycetemcomitans genes.
authors.affiliation: Department of Microbiology and Molecular Genetics, College of Medicine, University of Vermont, Burlington 05405, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Lippmann
authors.forename: E H
authors.initials: EH
authors.lastname: Froeliger
authors.forename: P M
authors.initials: PM
authors.lastname: Fives-Taylor
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics* / Q000378:metabolism*; D001431:Bacteriological Techniques; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005098:Exotoxins / Q000235:genetics; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic
chemical_list: D005098:Exotoxins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; C018579:leukotoxin; D049452:Green Fluorescent Proteins