title: Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis.
abstract: The random amplified polymorphic DNA (RAPD) technique was employed in the haplo-diploid dioecious species Gracilaria gracilis to identify sex-linked PCR markers. Sixty-nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid females. One of these primers (OPD13) generated a 430-bp fragment specific to males and a 620-bp fragment specific to females. The diploid individuals (tetrasporophytes) showed the co-occurrence of these two fragments. In order to verify the linkage between the sexual phenotypes and these markers, a progeny array of 59 haploid individuals (male and female) born on a diploid individual was analysed, in all of which the two markers produced by the OPD13 primer segregated perfectly with sex.
authors.affiliation: Laboratorie de Génetique et Evolution des Populations Végétales, UPRESA CNRS 8016, GDR 1002, FR 1818, Villeneuve dAscq Cedex, France.
authors.forename: E A
authors.initials: EA
authors.lastname: Martinez
authors.forename: C
authors.initials: C
authors.lastname: Destombe
authors.forename: M C
authors.initials: MC
authors.lastname: Quillet
authors.forename: M
authors.initials: M
authors.lastname: Valero
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004171:Diploidy; D005819:Genetic Markers; D006238:Haploidy; D008969:Molecular Sequence Data; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique; D000461:Rhodophyta / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers