title: Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana.
abstract: A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2'-deacetoxydecinnamoyltaxinine J and 2'-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV-Vis as well as photodiode array detection.
authors.affiliation: Analytical Instrumentation, Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Bala
authors.forename: G C
authors.initials: GC
authors.lastname: Uniyal
authors.forename: S K
authors.initials: SK
authors.lastname: Chattopadhyay
authors.forename: V
authors.initials: V
authors.lastname: Tripathi
authors.forename: K V
authors.initials: KV
authors.lastname: Sashidhara
authors.forename: M
authors.initials: M
authors.lastname: Kulshrestha
authors.forename: R P
authors.initials: RP
authors.lastname: Sharma
authors.forename: S P
authors.initials: SP
authors.lastname: Jain
authors.forename: A K
authors.initials: AK
authors.lastname: Kukreja
authors.forename: S
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017239:Paclitaxel / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014197:Trees / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel