Mapping of capillary flow, cellular redox state, and resting membrane potential in hypoperfused rat myocardium.
The influence on myocyte viability of ischemia-induced changes in capillary perfusion was studied in the hearts of anesthetized rats subjected to partial occlusion of the left coronary artery for 45 min. Timed plasma labeling was applied to determine perfusion patterns. Changes in the fluorescence of preloaded potential-sensitive dyes [tetramethylrhodamine methyl ester (TMRM) and bis-oxonol], of trypan blue, and of endogeneous NADH were utilized in characterizing myocyte viability in histological sections of the heart. Within the hypoperfused zone, localized areas appeared vascularly nonlabeled for periods of at least 10 min. Within these areas a reduction in TMRM fluorescence occurred in 82. 5% of the tissue, signaling a reduced resting membrane potential. In the same areas 37.7% of the myocytes revealed an NADH fluorescence lower than that regularly found in anoxic tissues. This correlated with an especially low level of TMRM, with increased fluorescence bis-oxonol and with an accumulation of trypan blue. In conclusion, in localized hypoperfusion-induced zones lacking capillary flow, an inhomogeneous pattern of reductions in myocyte viability develops, which appears to be relevant in ischemia-induced arrhythmias.
Brasch
F
F
Zentrum Pharmakologie und Toxikologie, Forschung of the University, D-37075 Göttingen, Germany.
Neckel
M
M
Volkmann
R
R
Schmidt
G
G
Hellige
G
G
Vetterlein
F
F
D000818:Animals; D002196:Capillaries / Q000503:physiopathology; D002470:Cell Survival; D004396:Coloring Agents; D003326:Coronary Circulation; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D008297:Male; D008564:Membrane Potentials; D017202:Myocardial Ischemia / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D009243:NAD / Q000493:pharmacokinetics; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D012235:Rhodamines / Q000493:pharmacokinetics; D013858:Thiobarbiturates / Q000493:pharmacokinetics; D013997:Time Factors; D014018:Tissue Distribution; D014343:Trypan Blue
D004396:Coloring Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; D013858:Thiobarbiturates; C401833:tetramethylrhodamine methyl ester; D009243:NAD; C025284:bis(1,3-diethylthiobarbiturate)trimethineoxonol; D014343:Trypan Blue