title: Determination of hexamethylene diisocyanate-based isocyanates in spray-painting operations. Part 2. Comparison of high performance liquid chromatography with capillary zone electrophoresis.
abstract: A capillary zone electrophoresis (CZE) approach was developed for the determination of hexamethylene diisocyanate (HDI) monomer and HDI-based oligomers. A comparison of CZE with high performance liquid chromatography (HPLC) indicates that the CZE separation completely isolates isocyanates from excess solvent, derivatizing reagent and pigment while offering a fivefold increase in sensitivity. The CZE approach allows for the quantification of HDI monomer and oligomer within a 1 min time window under the run conditions selected. For the determination of HDI-based oligomer, provided that the relative response with respect to HDI monomer is calculated, there is no significant difference (p < 0.05, n = 10) in the isocyanate air concentration when using either HPLC or CZE. The results are significant because they indicate that CZE has advantages for the determination of both HDI-based oligomer and HDI monomer generated during spray-painting operations.
authors.affiliation: Department of Chemistry, Southwest Texas State University, San Marcos 78666, USA.
authors.forename: W E
authors.initials: WE
authors.lastname: Rudzinski
authors.forename: J
authors.initials: J
authors.lastname: Yin
authors.forename: E
authors.initials: E
authors.lastname: England
authors.forename: G
authors.initials: G
authors.lastname: Carlton
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003485:Cyanates / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D017953:Isocyanates; D010150:Paint / Q000032:analysis*
chemical_list: D000395:Air Pollutants, Occupational; D003485:Cyanates; D017953:Isocyanates; C015262:1,6-hexamethylene diisocyanate