A physical map of the chromosome of Xanthomonas campestris pv. phaseoli var. fuscans BXPF65.
A physical map of the Xanthomonas campestris pv. phaseoli var. fuscans BXPF65 chromosome was constructed by PFGE and Southern hybridization. The chromosome was 3938 kb and was composed of 39 XbaI restriction fragments. The size is close to that estimated for X. campestris pv. campestris based on DNA renaturation kinetics. Macrorestriction fragments containing genes for a 16S rRNA, pectate lyase, two-component regulatory system and hrp cluster were located on the physical map. Each of these probes hybridized to single macrorestriction fragments except the 16S rRNA probe, which hybridized to five fragments located in different areas on the chromosome map.
Chan
J W
JW
Department of Environmental Biology, University of Guelph, Guelph, Ont., Canada.
Goodwin
P H
PH
D015139:Blotting, Southern; D002876:Chromosomes, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D020161:Physical Chromosome Mapping; D012336:RNA, Ribosomal, 16S; D016959:Xanthomonas campestris / Q000235:genetics*
D012336:RNA, Ribosomal, 16S