title: Synergy testing of vancomycin-resistant Enterococcus faecium against quinupristin-dalfopristin in combination with other antimicrobial agents.
abstract: Using checkerboard and time-kill assays, we evaluated the in vitro activity of quinupristin-dalfopristin (RP 59500) alone and in combination with five other antimicrobial agents against 12 clinical strains of vancomycin-resistant Enterococcus faecium (VREF). In time-kill studies, six VREF strains exhibited synergism with the combination of quinupristin-dalfopristin and doxycycline and three exhibited synergism with quinupristin-dalfopristin plus ampicillin-sulbactam. Combinations of quinupristin-dalfopristin with these and other agents warrant further clinical evaluation for the treatment of serious VREF infections.
authors.affiliation: Department of Microbiology, SD Laboratory Services, Sunnybrook and Women's College Health Sciences Centre, North York, Canada.
authors.forename: S O
authors.initials: SO
authors.lastname: Matsumura
authors.forename: L
authors.initials: L
authors.lastname: Louie
authors.forename: M
authors.initials: M
authors.lastname: Louie
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D004357:Drug Synergism; D016984:Enterococcus faecium / Q000187:drug effects*; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D013997:Time Factors; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance; D014769:Virginiamycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D014769:Virginiamycin; C062940:quinupristin-dalfopristin; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases