title: Induction of apoptosis in AK-5 cells by rotenone involves participation of caspases.
abstract: AK-5 tumour cells undergo apoptosis after treatment with rotenone an electron transport inhibitor and oligomycin which inhibits mitochondrial ATPases. Apoptotic process involves the induction of caspases 2 and 3, whereas caspase 1 does not seem to be participating in rotenone/oligomycin induced apoptosis. DEVD which is a specific inhibitor of caspase 3, inhibited apoptosis in AK-5 cells. We have also observed a significant lowering of intracellular pH in AK-5 cells which are induced into the apoptotic process by rotenone. These results suggest an important role for mitochondrial electron transport in the induction of apoptosis in AK-5 tumour cells.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India. khar@ccmb.ap.nic.in
authors.forename: A
authors.initials: A
authors.lastname: Khar
authors.forename: A M
authors.initials: AM
authors.lastname: Ali
authors.forename: Z
authors.initials: Z
authors.lastname: Begum
authors.forename: B V
authors.initials: BV
authors.lastname: Pardhasaradhi
authors.forename: C
authors.initials: C
authors.lastname: Varalakshmi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012402:Rotenone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D012402:Rotenone; D020169:Caspases