Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells.
The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra- protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.
Ichino
N
N
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
Ishiguro
H
H
Yamada
K
K
Nishii
K
K
Sawada
H
H
Nagatsu
T
T
D000310:Adrenal Gland Neoplasms; D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology*; D016762:Genes, fos; D016758:Genes, jun; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009538:Nicotine / Q000494:pharmacology*; D015345:Oligonucleotide Probes; D016716:PC12 Cells; D010673:Pheochromocytoma; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013375:Substance Withdrawal Syndrome; D014158:Transcription, Genetic / Q000187:drug effects
D016375:Antisense Elements (Genetics); D015345:Oligonucleotide Probes; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D009538:Nicotine