title: Effects of hemolysis on plasma electrolyte and chemistry values in the common green iguana (Iguana iguana).
abstract: Hemolysis of serum and plasma samples is a common problem in veterinary diagnostic laboratories. We measured the effects of hemolysis on nine plasma analytes in 10 clinically normal common green iguanas (Iguana iguana). Blood samples with moderate and marked hemolysis were produced from each iguana by freezing, centrifuging, and decanting plasma from a portion of each blood sample, and combining the nonhemolyzed plasma with different amounts of hemolyzed plasma from the same individual. Moderate hemolysis significantly increased plasma phosphorus levels. Marked hemolysis significantly increased plasma values of potassium, phosphorus, total protein, and aspartate aminotransferase. The severity of hemolysis must be considered when interpreting values for these analytes in iguana plasma.
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
authors.forename: K G
authors.initials: KG
authors.lastname: Benson
authors.forename: J
authors.initials: J
authors.lastname: Paul-Murphy
authors.forename: P
authors.initials: P
authors.lastname: MacWilliams
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001774:Blood Chemical Analysis / Q000662:veterinary; D001798:Blood Proteins / Q000032:analysis; D002118:Calcium / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004573:Electrolytes / Q000097:blood*; D006461:Hemolysis / Q000502:physiology*; D007076:Iguanas / Q000097:blood*; D010758:Phosphorus / Q000097:blood; D011188:Potassium / Q000097:blood; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis; D012964:Sodium / Q000097:blood; D014527:Uric Acid / Q000097:blood
chemical_list: D001798:Blood Proteins; D004573:Electrolytes; D012709:Serum Albumin; D014527:Uric Acid; D010758:Phosphorus; D012964:Sodium; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D011188:Potassium; D002118:Calcium