Voltage-dependent interaction of the peptaibol antibiotic zervamicin II with phospholipid vesicles.
The effect of a transmembrane potential on ion channel formation by zervamicin II (ZER-II) was studied in a vesicular model system. The dissipation of diffusion potential caused by addition of ZER-II to small phosphatidylcholine vesicles was monitored using fluorescent (Safranine T) and optical (Oxonol YI) probes. Cis-positive potentials facilitated channel formation, while at cis-negative potentials, ion fluxes were inhibited. A potential-independent behavior of ZER-II was observed at high peptide concentrations, most likely due to its membrane modifying property.
Kropacheva
T N
TN
Chemistry Department, Udmurt State University, Izhevsk, Russia.
Raap
J
J
D000408:Alamethicin / Q000494:pharmacology; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D054848:Peptaibols; D010455:Peptides; D010743:Phospholipids / Q000502:physiology*; D014634:Valinomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D007473:Ion Channels; D054848:Peptaibols; D010455:Peptides; D010743:Phospholipids; C116937:peptaibolin; D014634:Valinomycin; D000408:Alamethicin; C077077:zervamicin IIB; C051145:zervamicin IIA