title: In vitro inducible nitric oxide synthesis inhibitors from Alismatis Rhizoma.
abstract: Bioassay-guided fractionation of an aqueous extract of Alismatis Rhizoma has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, alismol (1) and alisol B monoacetate (2), together with an inactive triterpene, alisol C monoacetate (3). Compounds 1 and 2 inhibited nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibitory effects of 1 and 2 on NO synthesis were partly due to suppression of iNOS mRNA expression as determined by Northern blotting.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Chonbuk, Korea.
authors.forename: N Y
authors.initials: NY
authors.lastname: Kim
authors.forename: T H
authors.initials: TH
authors.lastname: Kang
authors.forename: H O
authors.initials: HO
authors.lastname: Pae
authors.forename: B M
authors.initials: BM
authors.lastname: Choi
authors.forename: H T
authors.initials: HT
authors.lastname: Chung
authors.forename: S W
authors.initials: SW
authors.lastname: Myung
authors.forename: Y S
authors.initials: YS
authors.lastname: Song
authors.forename: D H
authors.initials: DH
authors.lastname: Sohn
authors.forename: Y C
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002783:Cholestenones / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000378:metabolism; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D002783:Cholestenones; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D012717:Sesquiterpenes; C401295:alisol B monoacetate; C053720:alismol; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse