title: Molecular characterization of human group C rotavirus genes 6, 7 and 9.
abstract: Genes 6, 7 and 9 of human group C rotavirus 'Bristol' strain, encoding non-structural proteins (NSP) 3, 1 and 2, respectively, were cloned and sequenced. Human group C rotavirus genome segment 6 is 1350 bp and contains a single ORF of 1231 nucleotides (encoding 402 amino acids). Genome segment 7 is 1270 bp and encodes a protein of 394 amino acids and genome segment 9 is 1037 bp and encodes a 312 amino acid protein. The human group C rotavirus genes 6, 7 and 9 showed 78, 67 and 88% sequence identity, respectively, to the corresponding porcine group C rotavirus genes. The derived protein sequences were compared with those of the porcine 'Cowden' group C and mammalian group A rotavirus strains. The human group C rotavirus NSP1 protein sequence is one amino acid longer than the porcine group C equivalent. In common with group A and porcine group C rotaviruses, the human group C rotavirus NSP1 protein has a zinc finger motif. Human group C rotavirus NSP2 has two hydrophobic heptad repeat regions, a basic, RNA-binding domain and a basic, proline-rich region. Human group C rotavirus NSP3 has both single- and double-stranded RNA-binding domains and several hydrophobic heptad repeat regions, one of which forms a leucine zipper. This work completes the molecular characterization of the non-structural proteins of a human group C rotavirus. Phylogenetic analysis of all the non-structural genes of group A, B and C rotaviruses suggests that these viruses have diverged at a constant rate from a common ancestor.
authors.affiliation: Public Health Laboratory1 and Department of Molecular Microbiology, University Medical School2, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK.
authors.forename: Vivienne L A
authors.initials: VLA
authors.lastname: James
authors.affiliation: Public Health Laboratory1 and Department of Molecular Microbiology, University Medical School2, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Lambden
authors.affiliation: Public Health Laboratory1 and Department of Molecular Microbiology, University Medical School2, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK.
authors.forename: Yu
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Regional Virus Laboratory, Public Health Laboratory, Myrtle Road, Bristol BS2 8EL, UK3.
authors.forename: E Owen
authors.initials: EO
authors.lastname: Caul
authors.affiliation: Public Health Laboratory1 and Department of Molecular Microbiology, University Medical School2, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK.
authors.forename: Ian N
authors.initials: IN
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D012401:Rotavirus / Q000235:genetics* / Q000378:metabolism; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; C082740:NSP3 protein, Rotavirus; D016601:RNA-Binding Proteins; D017361:Viral Nonstructural Proteins; C104282:nsp1 protein, Rotavirus; C079234:NS35 protein, rotavirus