Structure and chromosomal location of the rat gene encoding the heart fatty acid-binding protein.
The gene coding for rat heart fatty acid-binding protein (FABP), along with 1.2 kb of its 5'-untranscribed region, was amplified by PCR, cloned and sequenced. As in other FABP genes, the coding sequence is interrupted by three introns of 3.4, 1.4 and 1.1 kb, respectively. Fluorescence in situ hybridization mapping revealed that the gene is located on chromosome 5q36. Using intron-specific primers flanking exon 2, unspliced primary transcript RNA of the FABP gene was detected in a preparation of total RNA isolated from rat heart, proving that the cloned gene is expressed in adult cardiac tissue.
Zhang
J
J
Department of Biological Sciences, Simon Fraser University, Burnaby, Canada.
Rickers-Haunerland
J
J
Dawe
I
I
Haunerland
N H
NH
D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005091:Exons; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020121:5' Untranslated Regions; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger