UVC-induced cell death of IL-2-dependent human lymphocytes.
We compared the in vitro propensity of human IL-2-dependent lymphocytes (young proliferating and senescent non-proliferating), and resting peripheral blood lymphocytes (PBLs) to undergo UVC-induced apoptosis. The activities of AP-1 (activator protein-1), CRE (cAMP response element) and OCT-1 (octamer-1) transcription factors in all lymphocytes were also assessed. At 24 h after UVC treatment, half of young proliferating T lymphocytes and about a quarter of PBLs and senescent non-proliferating cells were apoptotic, as shown by flow cytometry. However, only in young lymphocytes were both typical DNA 'ladder' and Bcl-2 downregulation evident. The AP-1 transcription factor was activated by UVC in IL-2-dependent young and senescent, but not resting lymphocytes. Taken together, the data show different propensities of resting, proliferating and senescent human lymphocytes to undergo UVC-induced apoptosis and AP-1 activation.
Radziszewska
E
E
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Piwocka
K
K
Skierski
J
J
Sikora
E
E
D000328:Adult; D017209:Apoptosis / Q000528:radiation effects*; D002455:Cell Division; D016922:Cellular Senescence; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D053938:DNA Fragmentation / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism; D051863:Host Cell Factor C1; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D050811:Octamer Transcription Factor-1; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014466:Ultraviolet Rays; D051028:bcl-2-Associated X Protein
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D007376:Interleukin-2; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D051028:bcl-2-Associated X Protein