Excretion of (14)C-fumonisin B(1), (14)C-hydrolyzed fumonisin B(1), and (14)C-fumonisin B(1)-fructose in rats.
14C-Fumonisin B(1) (FB(1)) was produced by Fusarium proliferatum M-5991 in modified Myro liquid medium and purified to >95% purity with a specific activity of 1.7 mCi/mmol. Nine male and nine female F344/N rats were each dosed by gavage with 0.69 micromol of (14)C-FB(1), (14)C-hydrolyzed FB(1), or (14)C-FB(1)-fructose/kg body weight. Urinary excretion of (14)C-FB(1) and (14)C-FB(1)-fructose was 0.5% and 4.4% of the total dose, respectively, and was similar between male and female rats. Urinary excretion of (14)C-hydrolyzed HFB(1) was significantly greater (P > 0.05) in female rats as compared with male rats (17.3% vs 12.8% of the total dose, respectively). There were no significant (P > 0.05) differences in biliary excretion of the three fumonisin compounds with a mean of 1. 4% of the dose excreted at 4 h after dosing. Lesser amounts continued to be excreted up to 9.25 h after dosing. Although biliary excretion of the (14)C-FB(1), (14)C-hydrolyzed FB(1), and (14)C-FB(1)-fructose was similar, increased urinary excretion of the (14)C-hydrolyzed FB(1) as compared to (14)C-FB(1) and (14)C-FB(1)-fructose indicated a greater absorption of the hydrolyzed form.
Dantzer
W R
WR
Department of Food Science, Iowa State University, 2312 Food Sciences Building, Ames, Iowa 50011, USA.
Hopper
J
J
Mullin
K
K
Hendrich
S
S
Murphy
P A
PA
D000818:Animals; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000493:pharmacokinetics* / Q000652:urine; D002274:Carcinogens, Environmental / Q000493:pharmacokinetics*; D005260:Female; D005519:Food Preservation; D005632:Fructose / Q000031:analogs & derivatives / Q000493:pharmacokinetics* / Q000652:urine; D037341:Fumonisins; D006801:Humans; D008297:Male; D009183:Mycotoxins / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D012737:Sex Factors
D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; D005632:Fructose; C056933:fumonisin B1