title: Mutation spectrum induced by singlet oxygen in Escherichia coli deficient in exonuclease III.
abstract: The repair of singlet oxygen (1O2)-induced DNA lesions requires several enzymes of the nucleotide and base excision repair pathways, including exonuclease III and endonuclease IV that are known apurinic/apyrimidinic-endonucleases in Escherichia coli. In order to better understand the relevance of exonuclease III on the repair of these lesions, we investigated the mutagenic events that result from the replication of a 1O2-damaged plasmid in an exonuclease-deficient host (xth). The mutation spectrum in the tRNA supF gene target indicated that the absence of exonuclease III does not change the types of mutations induced by 1O2 (mostly of G:C-->T:A and G:C-->C:G transversions). However, the spectrum shows that the mutations are scattered in the supF gene, which is significatively different from the one obtained in wild-type bacteria. Thus, exonuclease III may act on the repair of 1O2-induced lesions altering the DNA repair sequence specificity.
authors.affiliation: Departamento de Microbiologia, Universidade de São Paulo, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Agnez-Lima
authors.forename: P
authors.initials: P
authors.lastname: Di Mascio
authors.forename: R L
authors.initials: RL
authors.lastname: Napolitano
authors.forename: R P
authors.initials: RP
authors.lastname: Fuchs
authors.forename: C F
authors.initials: CF
authors.lastname: Menck
mesh_terms: D001483:Base Sequence; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010100:Oxygen / Q000633:toxicity*; D018462:Photobiology; D010957:Plasmids / Q000235:genetics; D026082:Singlet Oxygen
chemical_list: D004269:DNA, Bacterial; D026082:Singlet Oxygen; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D010100:Oxygen