Biological functions of recombinant bovine interleukin 6 expressed in a baculovirus system.
The cDNA encoding bovine interleukin 6 (IL-6) was obtained from messenger RNA extracted from lipopolysaccharide-stimulated bovine Kupffer cells by the reverse transcription polymerase chain reaction (RT/PCR), and cloned into the baculovirus vector pVL 1392. Insect cells (Sf21AE derived from Spodoptera frugiperda) infected with the recombinant baculovirus secreted a large amount of 23.7 kD protein into the culture medium. This protein was capable of causing increased haptoglobin production and decreased albumin production in primary cultured bovine hepatocytes. The swine and human IL-6s were also able to decrease albumin production in bovine hepatocytes. This recombinant IL-6 did not stimulate the proliferation of 7TD1 cells (a murine IL-6-dependent cell line), whereas the recombinant swine IL-6 which was expressed in the same baculovirus system, and recombinant human IL-6 derived from Escherichia coli were each capable of stimulating proliferation of 7TD1 cells, respectively. This suggests a species restriction between bovine IL-6 and murine IL-6 dependent cell lines.
Yoshioka
M
M
National Institute of Animal Health, Tsukuba, Japan. miyako@niah.affrc.go.jp
Mori
Y
Y
Miyazaki
S
S
Miyamoto
T
T
Yokomizo
Y
Y
Nakajima
Y
Y
D000209:Acute-Phase Proteins / Q000096:biosynthesis; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D015153:Blotting, Western; D002417:Cattle; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006242:Haptoglobins / Q000096:biosynthesis; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D012709:Serum Albumin / Q000096:biosynthesis; D018411:Spodoptera; D013552:Swine; D014162:Transfection
D000209:Acute-Phase Proteins; D017077:Culture Media, Conditioned; D018076:DNA, Complementary; D006242:Haptoglobins; D015850:Interleukin-6; D011994:Recombinant Proteins; D012709:Serum Albumin