title: A multiple-capillary electrophoresis system for small-scale DNA sequencing and analysis.
abstract: A five-capillary system has been developed for DNA sequencing and analysis. The post-column fluorescence detector is based on a sheath-flow cuvette. The instrument provides uniform and continuous illumination of the samples. The cuvette virtually eliminates cross-talk in the fluorescence signal between capillaries. Discrete single-photon counting avalanche photodiodes provide high efficiency light detection. The instrument has detection limits (3sigma) of 130 +/- 30 fluorescein molecules injected onto each capillary. Over 650 bases of sequence at 98.8% accuracy were generated in 100 min at 50 degrees C from M13mp18. Separation and detection of short tandem repeats proved efficient and accurate with the use of internal standards for direct comparison of migration times between capillaries.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: K O
authors.initials: KO
authors.lastname: Voss
authors.forename: D F
authors.initials: DF
authors.lastname: Shaw
authors.forename: K P
authors.initials: KP
authors.lastname: Roos
authors.forename: D F
authors.initials: DF
authors.lastname: Lewis
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: R
authors.initials: R
authors.lastname: Jiang
authors.forename: H
authors.initials: H
authors.lastname: Ren
authors.forename: J Y
authors.initials: JY
authors.lastname: Hou
authors.forename: Y
authors.initials: Y
authors.lastname: Fang
authors.forename: X
authors.initials: X
authors.lastname: Puyang
authors.forename: H
authors.initials: H
authors.lastname: Ahmadzadeh
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D000328:Adult; D002648:Child; D002897:Chromosomes, Human, Pair 7; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005260:Female; D005336:Fiber Optic Technology; D005453:Fluorescence; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008853:Microscopy; D055095:Optics and Photonics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods; D020080:Tandem Repeat Sequences; D013696:Temperature