title: Negative regulation of the gene for Fe-containing superoxide dismutase by an Ni-responsive factor in Streptomyces coelicolor.
abstract: In Streptomyces coelicolor, transcription of the sodF genes, encoding Fe-containing superoxide dismutases, is negatively regulated by nickel. Gel mobility shift assays with sodF1 promoter fragments and cell extracts from the A3(2) strain indicate the presence of a nickel-responsive DNA-binding protein, most likely a transcriptional repressor. The boundary for the Ni-responsive cis-acting region was identified both in vitro and vivo. Ni does not regulate the level of the putative repressor but only the binding competence of this protein.
authors.affiliation: Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Chung
authors.forename: J H
authors.initials: JH
authors.lastname: Choi
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.forename: Y H
authors.initials: YH
authors.lastname: Cho
authors.forename: J H
authors.initials: JH
authors.lastname: Roe
mesh_terms: D019677:Artificial Gene Fusion; D003035:Cobalt / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D007501:Iron / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism; D009532:Nickel / Q000378:metabolism* / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics*; D015032:Zinc / Q000378:metabolism
chemical_list: D003035:Cobalt; D008345:Manganese; D003300:Copper; D009532:Nickel; D007501:Iron; D013482:Superoxide Dismutase; D015032:Zinc