title: Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis.
abstract: The human 15-lipoxygenase (15-LO) gene was transfected into rat kidneys in vivo via intra-renal arterial injection. Three days later, acute (passive) or accelerated forms of antiglomerular basement membrane antibody-mediated glomerulonephritis were induced in transfected and nontransfected or sham-transfected controls. Studies of glomerular functions (filtration and protein excretion) and ex vivo glomerular leukotriene B(4) biosynthesis at 3 hr, and up to 4 days, after induction of nephritis revealed preservation or normalization of these parameters in transfected kidneys that expressed human 15-LO mRNA and mature protein, but not in contralateral control kidneys or sham-transfected animals. The results provide in vivo-derived data supporting a direct anti-inflammatory role for 15-LO during immune-mediated tissue injury.
authors.affiliation: Center for Glomerulonephritis, Renal Division, Emory University and Veterans Administration Medical Center, Atlanta, GA 30033, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Munger
authors.forename: A
authors.initials: A
authors.lastname: Montero
authors.forename: M
authors.initials: M
authors.lastname: Fukunaga
authors.forename: S
authors.initials: S
authors.lastname: Uda
authors.forename: T
authors.initials: T
authors.lastname: Yura
authors.forename: E
authors.initials: E
authors.lastname: Imai
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneda
authors.forename: J M
authors.initials: JM
authors.lastname: Valdivielso
authors.forename: K F
authors.initials: KF
authors.lastname: Badr
mesh_terms: D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics*; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis; D015316:Genetic Therapy; D005919:Glomerular Filtration Rate; D005921:Glomerulonephritis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007975:Leukotriene B4 / Q000031:analogs & derivatives / Q000096:biosynthesis; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D007975:Leukotriene B4; C040212:leukotriene B5; D015118:Eicosapentaenoic Acid; D001093:Arachidonate 15-Lipoxygenase