Simultaneous determination of cis- and trans-resveratrol in wines by capillary zone electrophoresis.
A capillary zone electrophoresis method for determining cis- and trans-resveratrol isomers is proposed. Optimal conditions for the quantitative separation were investigated. A background electrolyte solution consisting of 40 mM borate buffer adjusted to pH 9.5, hydrodynamic injection and 5 kV of separation voltage were used. Good linearity and precision were obtained for the two isomers. Detection limits of 0.06 mg L-1 for trans-resveratrol and 0.08 mg L-1 for cis-reveratrol were obtained. The developed method is rapid and sensitive and it has been applied to determine cis- and trans-resveratrol in several red wines. The samples were purified and enriched by passing them through a preconditioned C18 cartridge and eluting the isomers with acetonitrile-water (3 + 7).
Berzas Nevado
J J
JJ
Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, Ciudad Real, Spain.
Contento Salcedo
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Castañeda Peñalvo
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D016588:Anticarcinogenic Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D016588:Anticarcinogenic Agents; D013267:Stilbenes; D000077185:Resveratrol