Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1.
Growth of Streptomyces clavuligerus NP1 in the presence of methanol or ethanol resulted in a marked increase in production of cephalosporin(s) from penicillin G by resting cells. The mycelium produced in alcohol-supplemented medium was fragmented and dispersed as compared with growth in control medium. HPLC analysis showed that at least two products were present in the biotransformation supernatant fluid after 1 h incubation. One of them has been identified as deacetoxycephalosporin G (DAOG).
Fernández
M J
MJ
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Adrio
J L
JL
Piret
J M
JM
Wolfe
S
S
Ro
S
S
Demain
A L
AL
D000438:Alcohols / Q000494:pharmacology*; D001711:Biotransformation / Q000187:drug effects; D002511:Cephalosporins / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D010400:Penicillin G / Q000378:metabolism*; D010406:Penicillins / Q000378:metabolism*; D013302:Streptomyces / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
D000438:Alcohols; D002511:Cephalosporins; D003470:Culture Media; D010406:Penicillins; D000431:Ethanol; D010400:Penicillin G; D000432:Methanol