Cloning and characterization of a rabbit ortholog of human Galpha16 and mouse G(alpha)15.
A cDNA was cloned from a rabbit spleen cDNA library which encoded a G-protein alpha subunit peptide of 374 amino acids, that at the peptide level exhibited 86% and 79% identity with human Galpha16 and mouse G(alpha)15, respectively. The rabbit G(alpha)subunit cDNA was subcloned into a mammalian expression vector and transiently co-transfected into HEK-293 cells along with cDNAs encoding the human C3a, C5a, or nociceptin/orphanin FQ receptors. In all three cases the rabbit G alpha subunit behaved similarly to G(alpha)15 or G(alpha)16 and effectively coupled the transfected receptors to intracellular calcium mobilization pathways. By nucleotide sequence homology and functional activity the rabbit G(alpha) subunit appears to be the ortholog of human G(alpha)16 and mouse G(alpha)15.
Feild
J A
JA
Department of Molecular Biology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, USA.
Foley
J J
JJ
Testa
T T
TT
Nuthulaganti
P
P
Ellis
C
C
Sarau
H M
HM
Ames
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D015723:Gene Library; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000235:genetics; D011957:Receptors, Opioid / Q000235:genetics; D016415:Sequence Alignment; D013154:Spleen / Q000378:metabolism; D014162:Transfection; D000094942:Nociceptin Receptor
D015703:Antigens, CD; D008565:Membrane Proteins; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D011957:Receptors, Opioid; C085567:complement C3a receptor; C112631:G protein alpha 16; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium; D000094942:Nociceptin Receptor