title: Localisation of the cell wall-associated phosphodiesterase PhoD of Bacillus subtilis.
abstract: The localisation of phosphate-starvation-induced phosphodiesterase PhoD from Bacillus subtilis was studied by analysing processing, release and immunogold labelling of the sections. Although the processing of the pre-protein was extremely slow, the major fraction of PhoD could be detected at the surface of the cell wall. The results indicate that inefficient processing of the translocated pre-protein keeps PhoD in a cell wall-associated location. The uncleaved signal peptide might function as a membrane anchor.
authors.affiliation: Institute of Molecular Biology, Jena University, Winzerlaer Str. 10, D-07745, Jena, Germany. jmueller@imb-jena.de
authors.forename: J P
authors.initials: JP
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Wagner
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D015153:Blotting, Western; D002473:Cell Wall / Q000201:enzymology / Q000648:ultrastructure; D003470:Culture Media; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010710:Phosphates / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000032:analysis* / Q000378:metabolism; D011233:Precipitin Tests
chemical_list: D000907:Antibodies, Bacterial; D003470:Culture Media; D010710:Phosphates; D010727:Phosphoric Diester Hydrolases