Application of a marker of ciliated epithelial cells to gynaecological pathology.
BACKGROUND
The assessment of neoplastic disease in gynaecological histopathology can be complicated by the high incidence of metaplasia seen in tissues of the female genital tract. There is a need to identify specific tissue markers which can be applied in routine histopathological practice.


OBJECTIVE
To examine the clinical potential of a monoclonal antibody, LhS28, which reacts with basal bodies of ciliated epithelial cells.


METHODS
A panel of normal and pathological gynaecological tissues was processed and labelled with LhS28.


RESULTS
LhS28 immunoreactivity was found in the normal Fallopian tube where it was confined to ciliated rather than secretory epithelial cells. In the remaining specimens, LhS28 was associated exclusively with ciliated cells in tubal metaplasias of the cervix and endometrium and in benign serous lined inclusion cysts.


CONCLUSIONS
LhS28 may be a valuable marker for identifying metaplasia of tubal type and may find application in distinguishing tubal metaplasia from low grade cervical glandular intraepithelial neoplasia.
Comer
M T
MT
Imperial Cancer Research Fund Cancer Medicine Research Unit, St James's University Hospital, Leeds, UK.
Andrew
A C
AC
Leese
H J
HJ
Trejdosiewicz
L K
LK
Southgate
J
J
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005833:Genital Neoplasms, Female / Q000378:metabolism*; D005836:Genitalia, Female / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008679:Metaplasia / Q000378:metabolism; D011230:Precancerous Conditions / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor