Effect of photodynamic therapy on normal fibroblasts and colon anastomotic healing in mice.
Photodynamic therapy as an adjuvant modality to surgical resection of colon cancer is feasible provided that it does not affect healing of the anastomosis. The aim of this study was to evaluate the effects of photodynamic therapy on the viability of normal fibroblasts and on the healing process of colonic anastomosis in mice. Both in vitro and in vivo methods were employed. For in vitro study, 2 x 10(to the fifth power); human fibroblasts were incubated in triplicate with 5-aminolevulinic acid (2.5 microg/well) for 48 hours. Cells then underwent photoradiation at light doses of 50, 100, and 200 joules/cm(2) using a nonlaser light source. Viability was assessed by methylene blue dye exclusion. For in vivo studies, 60 mice were randomized into study and control groups and underwent laparotomy involving colonic anastomosis. The anastomosis underwent photodynamic therapy using 5-aminolevulinic acid (60 mg/kg) as a photosensitizer and a nonlaser light (40 joules/cm(2)). On postoperative days 1, 4, 7, 14, and 21, six mice were killed and subjected to bursting pressure and histologic examinations. Results of in vitro study showed pretreatment cell viability to be 96% to 99% in both groups. Photodynamic therapy caused no significant change in fibroblast viability at all light doses. Results of in vivo studies showed that the mean bursting pressure of both groups dropped to a low peak on day 4. Subsequently there was a gradual increase in bursting pressure along the examined time points (P <0. 001). There was no difference in bursting pressure between the two groups for all time points examined. It was concluded that photodynamic therapy has no effect on viability of normal human fibroblasts and no adverse effects on healing of colonic anastomosis.
Haddad
R
R
Department of Surgery,Tel Aviv Medical Center, Tel Aviv, Israel.
Kaplan
O
O
Brazovski
E
E
Rabau
M
M
Schneebaum
S
S
Shnaper
A
A
Skornick
Y
Y
Kashtan
H
H
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000714:Anastomosis, Surgical; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003106:Colon / Q000187:drug effects / Q000502:physiology / Q000601:surgery*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013529:Surgical Wound Dehiscence / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid