title: Surfactant protein A binds to the fusion glycoprotein of respiratory syncytial virus and neutralizes virion infectivity.
abstract: Collectins are a family of calcium-dependent collagenous lectins that appear to be important in innate host defense. We investigated the ability of three human collectins, namely, lung surfactant proteins A (SP-A) and D (SP-D) and the serum mannose-binding protein (MBP), to bind to the surface glycoproteins of respiratory syncytial virus (RSV). SP-A was shown to bind to the F (fusion) glycoprotein but not to the viral G (attachment) glycoprotein, and binding was completely abrogated in the presence of EDTA. Neither SP-D nor MBP bound to either glycoprotein. SP-A also neutralized RSV in a calcium dependent fashion. These results support a role for SP-A in the defense of infants against infection with RSV and indicate a possible mechanism for its protective activity.
authors.affiliation: Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Ghildyal
authors.forename: C
authors.initials: C
authors.lastname: Hartley
authors.forename: A
authors.initials: A
authors.lastname: Varrasso
authors.forename: J
authors.initials: J
authors.lastname: Meanger
authors.forename: D R
authors.initials: DR
authors.lastname: Voelker
authors.forename: E M
authors.initials: EM
authors.lastname: Anders
authors.forename: J
authors.initials: J
authors.lastname: Mills
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D037222:Collectins; D006023:Glycoproteins / Q000378:metabolism; D015707:HN Protein; D006801:Humans; D009500:Neutralization Tests; D011510:Proteolipids / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits; D012136:Respiratory Syncytial Viruses / Q000378:metabolism* / Q000502:physiology; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D037222:Collectins; D006023:Glycoproteins; D015707:HN Protein; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G