19F-MRI in vivo determination of the partial oxygen pressure in perfluorocarbon-loaded alginate capsules implanted into the peritoneal cavity and different tissues.
Semipermeable hydrogels formed with a biocompatible alginate solution and Ba(2+) ions protect encapsulated cells and tissues from a foreign immune system. For the viability and metabolic activity of the encapsulated materials, a sufficient oxygen supply inside the capsules is necessary. Quantitative (19)F-MRI was performed on perfluorocarbon-loaded alginate capsules implanted into the peritoneal cavity, the musculus quadriceps femoris, and beneath the kidney capsule of rats, in order to determine in vivo the partial oxygen pressure (pO(2)) inside the capsules at these implantation sites. The temporal behavior of the pO(2) values was observed for at least 3 months. The most stable values over time were observed in the kidney, where inter-rat pO(2) differences were considerable. In the muscle, the values were very high directly after implantation and decreased to nearly zero after 2 weeks. In the peritoneal cavity, values changed randomly over a wide range between different rats and over time. Magn Reson Med 42:1039-1047, 1999.
Nöth
U
U
Physikalisches Institut, Lehrstuhl für Biophysik, Am Hubland, Würzburg, Germany.
Gröhn
P
P
Jork
A
A
Zimmermann
U
U
Haase
A
A
Lutz
J
J
D000464:Alginates; D000818:Animals; D001672:Biocompatible Materials; D002214:Capsules; D004337:Drug Carriers; D005260:Female; D005461:Fluorine; D005466:Fluorocarbons; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007668:Kidney; D008279:Magnetic Resonance Imaging; D018482:Muscle, Skeletal; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D010529:Peritoneal Cavity; D051381:Rats; D017208:Rats, Wistar
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D004337:Drug Carriers; D005466:Fluorocarbons; D006603:Hexuronic Acids; D005461:Fluorine; D020723:Glucuronic Acid; D010100:Oxygen