title: Differential location of peptide hormones in the secretory pathway of insect adipokinetic cells.
abstract: Immunoreactivity of granules containing secretory material in the adipokinetic cells of the insect Locusta migratoria was studied using antisera specific for the adipokinetic hormone-associated peptides (AAP) I, II and III. Immunocytochemical detection of these associated peptides represents a new strategy for studying the intracellular location of the adipokinetic hormones and their prohormones. Fixation with 2% glutaraldehyde and 2% formaldehyde with low-temperature embedding in Lowicryl HM20 allowed highly selective immunogold labelling of both secretory and intracisternal granules. All three associated peptides were co-localized in secretory granules. This indicates that also all three adipokinetic hormones can be co-localized in these granules, which was confirmed by experiments in which, after secretory stimulation, adipokinetic hormone III was released from the adipokinetic cells together with adipokinetic hormones I and II. The immunopositivity of the intracisternal granules was similar to that of the secretory granules, although with the exception that the intracisternal granules did not show any specific reaction with anti-AAP III. The presence of AAP I and AAP II in intracisternal granules indicates that these granules only function as stores of adipokinetic prohormones I and II and not of adipokinetic prohormone III. The observed differences in storage in intracisternal granules among the three adipokinetic prohormones suggest differences in physiological significance of the three adipokinetic hormones in L. migratoria.
authors.affiliation: Biochemical Physiology Research Group and Institute of Biomembranes, Utrecht University, The Netherlands. l.f.harthoorn@bio.uu.nl
authors.forename: L F
authors.initials: LF
authors.lastname: Harthoorn
authors.forename: J H
authors.initials: JH
authors.lastname: Diederen
authors.forename: R C
authors.initials: RC
authors.lastname: Oudejans
authors.forename: D J
authors.initials: DJ
authors.lastname: Van der Horst
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006110:Grasshoppers / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data
chemical_list: D007301:Insect Hormones; C014956:adipokinetic hormone (locust); D000242:Cyclic AMP