PCR amplification of the gene acmA differentiates Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris.
The occurrence of the acmA gene, encoding the lactococcal N-acetylmuramidase in new lactococcal isolates from raw milk cheeses, has been determined. Isolates were genotypically identified to the subspecies level with a PCR technique. On the basis of PCR amplification of the acmA gene, the presence or absence of an additional amplicon of approximately 700 bp correlated with Lactococcus lactis subspecies. L. lactis subsp. lactis exhibits both the expected 1,131-bp product and the additional amplicon, whereas L. lactis subsp. cremoris exhibits a single 1,131-bp fragment.
Garde
S
S
Departamento de Tecnología de Alimentos, INIA, 28040 Madrid, Spain.
Babin
M
M
Gaya
P
P
Nuñez
M
M
Medina
M
M
D000818:Animals; D002611:Cheese / Q000382:microbiology; D017353:Gene Deletion; D013294:Lactococcus lactis / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D008892:Milk / Q000382:microbiology; D009113:Muramidase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
C092497:AcmA protein, Lactococcus lactis; D009113:Muramidase