Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease.
We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.
Takahashi
H
H
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp
Iwata
T
T
Kitagawa
Y
Y
Takahashi
R H
RH
Sato
Y
Y
Wakabayashi
H
H
Takashima
M
M
Kido
H
H
Nagashima
K
K
Kenney
K
K
Gibbs
C J
CJ
Kurata
T
T
D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D007536:Isomerism; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D011506:Proteins; D014446:Tyrosine 3-Monooxygenase