[In vitro study on MT2 cell infected by hepatitis C virus].
OBJECTIVE
To establish an MT-2 cell model infected by HCV in vitro.


METHODS
Human T-lymphocyte cell line MT-2 was infected with HCV-RNA positive serum and detected for HCV RNA by RT-PCR 1, 4, 7, 10, 14, 18, 21, 24, 28, 31, 35 and 39 days after the infection. PCR products were determined by southern blot. MT-2 cell infected with HCV-RNA negative serum was used as control.


RESULTS
HCV-RNA began to turn positive at 7 days postinoculate (p.i.) till at least 28 days(p.i.). Intracellular negative-stranded HCV-RNA was detected from 10 to 18 days (p.i.). No HCV RNA was detected in the control group.


CONCLUSIONS
HCV may replicate and express in MT-2 cell for a short period.
Chen
Z
Z
Institute of Infectious Diseases, First Affiliated Hospital, Medical Collage Zhejiang University, Hangzhou.
Liu
Y
Y
Chen
M
M
D000328:Adult; D002460:Cell Line; D005260:Female; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000821:virology*; D014779:Virus Replication
D012367:RNA, Viral