title: Effect of thermal injury with Pseudomonas aeruginosa infection on pulmonary and systemic bacterial clearance.
abstract: BACKGROUND
  Despite improvements in burn wound care, infections, particularly pneumonia, remain a major hurdle to recovery from thermal injury. After burns, a variety of systemic immune and inflammatory changes contribute to the risk of infection. Clinically, infection coupled with burn injury seems to adversely affect susceptibility to subsequent infection.
  
  
  METHODS
  Using a mouse model of 10% total body surface area, full-thickness, third-degree burns with quantitative bacterial cultures of multiple tissues, the effect of graded intratracheal and intraperitoneal infections with Pseudomonas aeruginosa on the development of infection was assessed.
  
  
  RESULTS
  P. aeruginosa infection of blood and lung were demonstrated in burned mice 4 hours after they received 1 to 7.2x10(5) P. aeruginosa intratracheally but not in unburned control mice. Disseminated infection from endogenous bacterial species (Proteus, Enterococcus, Streptococcus) involving the lungs, liver, blood, and subeschar space was observed in mice that received both burns and infection with P. aeruginosa (intraperitoneally and intratracheally) but not with infection or burn alone (p<0.01). After burns, pulmonary bacterial clearance was delayed in association with both pulmonary infection (7.2x10(5) P. aeruginosa intratracheally) and intraperitoneal infection (10(7) P. aeruginosa intraperitoneally). Histologically, diffuse pneumonitis was observed in mice that received burns and infection but not in mice with either infection or burns alone.
  
  
  CONCLUSIONS
  Small thermal injuries coupled with transient infection of the lungs or peritoneum delay the clearance of bacteria from the lungs and contribute to infection of the lungs, liver, burn site, and blood by endogenous organisms. These studies support the synergy of relatively small thermal injuries with infectious exposures in the pathogenesis of pneumonia and systemic infections after burns.
authors.affiliation: Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Wilkinson
authors.forename: J A
authors.initials: JA
authors.lastname: Fishman
mesh_terms: D000818:Animals; D002056:Burns / Q000276:immunology*; D003428:Cross Infection / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000276:immunology; D018410:Pneumonia, Bacterial / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012307:Risk Factors; D018805:Sepsis / Q000276:immunology*; D014946:Wound Infection / Q000276:immunology*