In vitro inducible nitric oxide synthesis inhibitors from Alismatis Rhizoma.
Bioassay-guided fractionation of an aqueous extract of Alismatis Rhizoma has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, alismol (1) and alisol B monoacetate (2), together with an inactive triterpene, alisol C monoacetate (3). Compounds 1 and 2 inhibited nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibitory effects of 1 and 2 on NO synthesis were partly due to suppression of iNOS mRNA expression as determined by Northern blotting.
Kim
N Y
NY
College of Pharmacy, Wonkwang University, Iksan, Chonbuk, Korea.
Kang
T H
TH
Pae
H O
HO
Choi
B M
BM
Chung
H T
HT
Myung
S W
SW
Song
Y S
YS
Sohn
D H
DH
Kim
Y C
YC
D000818:Animals; D002478:Cells, Cultured; D002783:Cholestenones / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000378:metabolism; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D002783:Cholestenones; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D012717:Sesquiterpenes; C401295:alisol B monoacetate; C053720:alismol; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse