Large scale determination of glucosinolates in brussels sprouts samples after degradation of endogenous glucose.
A method was developed for the determination of the glucosinolate content in glucose-rich samples of Brassica vegetables such as Brussels sprouts. Glucose in the samples was enzymatically degraded by the enzyme glucose oxidase (GOD). The resulting hydrogen peroxide and the enzyme GOD were thereafter respectively dissociated and inactivated by a heat treatment at 100 degrees C. After the degradation of endogenous glucose the glucosinolates were converted into glucose and related metabolites with the enzyme thioglucosidase originating from Brussels sprouts seeds. Glucose released was determined enzymatically with a glucose oxidase/peroxidase assay as a measure for the glucosinolate content of samples. The method was used to study the influence of harvest time, crop production location, and the choice of parental lines on the glucosinolate content of Brussels sprouts F1-hybrids. The sum of sinigrin and progoitrin of F1-hybrids was found to be significantly correlated to the glucosinolate content.
van Doorn
H E
HE
Novartis Seeds BV, Department of Biotechnology, P.O. Box 2, 1600 AA Enkhuizen, The Netherlands. hansvan.doorn@seeds.novartis.com
van der Kruk
G C
GC
van Holst
G J
GJ
D001937:Brassica / Q000737:chemistry* / Q000201:enzymology; D005947:Glucose; D005949:Glucose Oxidase; D005961:Glucosinolates / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D007202:Indicators and Reagents; D012639:Seeds / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods
D005961:Glucosinolates; D007202:Indicators and Reagents; D005949:Glucose Oxidase; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D005947:Glucose