title: New segmental long bone defect model in sheep: quantitative analysis of healing with dual energy x-ray absorptiometry.
abstract: An appropriate animal model is required for the study of treatments that enhance bone healing. A new segmental long bone defect model was developed for this purpose, and dual energy x-ray absorptiometry was used to quantify healing of this bone defect. In 15 sheep, a 3-cm segmental defect was created in the left tibia and fixed with an interlocking intramedullary nail. In seven animals, the defect was left empty for the assessment of the spontaneous healing response. In eight animals serving as a positive control, autologous bone grafting was performed. After 12 weeks, healing was evaluated with radiographs, a torsional test to failure, and dual energy x-ray absorptiometry. The mechanical test results were used for the assessment of unions and nonunions. Radiographic determination of nonunion was not reliably accomplished in this model. By means of dual energy x-ray absorptiometry, bone mineral density and content were measured in the middle of the defect. Bone mineral density was 91+/-7% (mean +/- SEM) and 72+/-6% that of the contralateral intact tibia in, respectively, the autologous bone-grafting and empty defect groups (p = 0.04). For bone mineral content, the values were, respectively, 117+/-18 and 82+/-9% (p = 0.07). Torsional strength and stiffness were also higher, although not significantly, in the group with autologous bone grafting than in that with the empty defect. Bone mineral density and content were closely related to the torsional properties (r2 ranged from 0.76 to 0.85, p < or = 0.0001). Because interlocking intramedullary nailing is a very common fixation method in patients, the newly developed segmental defect model has clinical relevance. The interlocking intramedullary nail provided adequate stability without implant failure. This model may be useful for the study of treatments that affect bone healing, and dual energy x-ray absorptiometry may be somewhat helpful in the analysis of healing of this bone defect.
authors.affiliation: Department of Traumatology, Free University Hospital, Amsterdam, The Netherlands. fcdenboer@hotmail.com
authors.forename: F C
authors.initials: FC
authors.lastname: den Boer
authors.forename: P
authors.initials: P
authors.lastname: Patka
authors.forename: F C
authors.initials: FC
authors.lastname: Bakker
authors.forename: B W
authors.initials: BW
authors.lastname: Wippermann
authors.forename: A
authors.initials: A
authors.lastname: van Lingen
authors.forename: G Q
authors.initials: GQ
authors.lastname: Vink
authors.forename: K
authors.initials: K
authors.lastname: Boshuizen
authors.forename: H J
authors.initials: HJ
authors.lastname: Haarman
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D004195:Disease Models, Animal; D005260:Female; D012044:Regression Analysis; D012756:Sheep; D013525:Surgical Instruments; D013978:Tibial Fractures / Q000000981:diagnostic imaging / Q000503:physiopathology*; D014102:Torsion Abnormality; D014945:Wound Healing / Q000502:physiology*