title: C5a receptor expression by TGW neuroblastoma cells.
abstract: We recently reported that not only lymphoid cells, but cells of neuronal origin may harbor C5a receptors (C5aR) as suggested by results of RT-PCR testing and that an apoptotic pathway is associated with the C5aR. To determine whether C5aR is expressed as an integral membrane protein, we generated mono- and polyclonal anti-C5aR antibodies. Flow cytometry showed a low-level expression of C5aR in TGW neuroblastoma cells. Epitope mapping suggested that a conformation change in C5aR occurs when exposed to C5a. Although an aphysiologically high concentration of C5a is necessary for inducing a transient increase in the intracellular Ca2+ level, TGW cells do employ the signal transduction pathway associated with C5aR, suggesting that these cells may serve as putative model for C5aR-expressing neurons.
authors.affiliation: Department of Molecular Biology, Nagoya City University School of Medicine, Nagoya, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Farkas
authors.forename: L
authors.initials: L
authors.lastname: Baranyi
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Z
authors.initials: Z
authors.lastname: Liposits
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Okada
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis*; D001932:Brain Neoplasms / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D015936:Complement C5a / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics; D017403:In Situ Hybridization; D009447:Neuroblastoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D007075:Immunoglobulin M; D012333:RNA, Messenger; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D014409:Tumor Necrosis Factor-alpha; D015936:Complement C5a; D002118:Calcium