Eosinophil activation and in situ interleukin-5 production by mononuclear cells in skin lesions of patients with drug hypersensitivity.
In order to determine the inflammatory mechanisms of skin lesions in patients with drug hypersentivity, we examined eosinophil activation and interleukin-5 (IL-5) production in infiltrating lymphocytes. First, we showed that the number of peripheral eosinophils and the level of serum IL-5 at the eruption-active stage were both significantly higher than those in healed skin eruptions. Histological and immunohistological examination revealed that CD4+ T cells and eosinophils significantly more densely infiltrated drug eruptions than control skin lesions. The infiltrating eosinophils were also shown to be activated by immunostaining using anti-secreted formed eosinophilic cationic protein monoclonal antibody. The expression of mRNA for IL-5 in the infiltrating mononuclear cells at drug eruptions was shown by in situ hybridization. These results suggest that infiltrating CD4+ T cells might regulate both peripheral and tissue eosinophils and facilitate allergic inflammation at drug eruptions by means of IL-5 production.
Mikami
C
C
Department of Dermatology, Toho University School of Medicine, Sakura Hospital, Japan.
Ochiai
K
K
Umemiya
K
K
Matsumura
R
R
Kagami
M
M
Tomioka
H
H
Sato
Y
Y
Tanabe
E
E
D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D016516:CD4-CD8 Ratio; D003875:Drug Eruptions / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D004802:Eosinophilia / Q000139:chemically induced* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000473:pathology
D015848:Interleukin-5