title: Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters.
abstract: Twenty-five non-identified fluorescent Pseudomonas strains isolated from natural mineral waters were previously clustered into three phenotypic subclusters, XIIIb, XVa and XVc. These strains were characterized genotypically in the present study. DNA-DNA hybridization results and DNA base composition analysis revealed that these strains were members of two new species, for which the names Pseudomonas gessardii sp. nov. (type strain CIP 105469T) and Pseudomonas migulae sp. nov. (type strain CIP 105470T) are proposed. P. gessardii included 13 strains from phenotypic subclusters XVa and XVc. P. migulae included 10 strains from phenotypic subcluster XIIIb. The levels of DNA-DNA relatedness ranged from 71 to 100% for P. gessardii and from 74 to 100% for P. migulae. The G + C content of the DNA of each type strain was 58 mol%. DNA similarity levels, measured with 67 reference strains of Pseudomonas species, were below 55%, with delta Tm values of 13 degrees C or more. The two new species presented basic morphological characteristics common to all pseudomonads. Various phenotypic features were found to differentiate them: P. gessardii strains utilized L-arabitol, myo-inositol, adonitol, xylitol and meso-erythritol as carbon sources, whereas P. migulae strains assimilated L-arabinose, D-xylose, D-saccharate, meso-tartrate, tricarballylate, D-glucuronate, D-galacturonate, phenylacetate and histamine. The complete 16S rRNA sequences of each type strain were determined and compared with those of the type strains of Pseudomonas species. Finally, a phylogenetic tree was inferred from sequence analysis and demonstrated that the two new species fell into the 'Pseudomonas fluorescens intrageneric cluster'. To date, their clinical significance is unknown.
authors.affiliation: Service de Bactériologie-Hygiène, Faculté de Médecine Henri Warembourg (pôle recherche), Verdun, Lille, France.
authors.forename: S
authors.initials: S
authors.lastname: Verhille
authors.forename: N
authors.initials: N
authors.lastname: Baïda
authors.forename: F
authors.initials: F
authors.lastname: Dabboussi
authors.forename: M
authors.initials: M
authors.lastname: Hamze
authors.forename: D
authors.initials: D
authors.lastname: Izard
authors.forename: H
authors.initials: H
authors.lastname: Leclerc
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008900:Mineral Waters / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008900:Mineral Waters; D012336:RNA, Ribosomal, 16S