Localization of 5-oxo-L-prolinase mRNA in the murine choroid plexus by in situ hybridization.
The gamma-glutamyl cycle is thought to play a significant role in the transport of amino acids between the blood and the cerebrospinal fluid. In the present study we investigated the expression of 5-oxo-L-prolinase, an enzyme of this pathway, in mouse brain and kidney by in situ hybridization using digoxygenin-labeled RNA probes. Expression of 5-OPase mRNA was found in epithelial cells of the proximal tubules in kidney as well as in the epithelium and vascular endothelial cells of the choroid plexus but not in periventricular capillaries.
Jäger
M
M
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Wolf
S
S
D000581:Amidohydrolases / Q000235:genetics*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002196:Capillaries / Q000201:enzymology; D002831:Choroid Plexus / Q000098:blood supply / Q000166:cytology / Q000201:enzymology*; D015342:DNA Probes; D004076:Digoxigenin; D004730:Endothelium, Vascular / Q000201:enzymology; D004847:Epithelial Cells / Q000201:enzymology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D020547:Lateral Ventricles / Q000166:cytology / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000235:genetics
D000596:Amino Acids; D015342:DNA Probes; D012333:RNA, Messenger; D005723:gamma-Glutamyltransferase; D000581:Amidohydrolases; C118889:5-oxoprolinase (ATP-hydrolyzing); D004076:Digoxigenin