title: Purification, crystallization, and preliminary X-ray characterization of a 36 kDa amaranth globulin.
abstract: The purpose of this study was to purify, crystallize, and characterize by X-ray diffraction an amaranth globulin for its subsequent structure elucidation. A 36-kDa amaranth globulin was extracted by sequential precipitation and purified by gel filtration and cationic exchange columns. It was crystallized at 18 degrees C from 4 M sodium formate. Suitable crystals for X-ray analysis were found to belong to the tetragonal crystal system with cell dimensions of a = b = 195.5 A and c = 164.14 A. Two possible tetragonal space groups P4(1)2(1)2 or P4(3)2(1)2 were determined. The crystals diffracted up to 2.5 A.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Zacatenco, Avenida Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360 Mexico, D.F., Departamento de.
authors.forename: N L
authors.initials: NL
authors.lastname: Vasco-Méndez
authors.forename: M
authors.initials: M
authors.lastname: Soriano-García
authors.forename: A
authors.initials: A
authors.lastname: Moreno
authors.forename: R
authors.initials: R
authors.lastname: Castellanos-Molina
authors.forename: O
authors.initials: O
authors.lastname: Paredes-López
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D018360:Crystallography, X-Ray; D005916:Globulins / Q000737:chemistry* / Q000302:isolation & purification; D010945:Plants, Edible / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*
chemical_list: D005916:Globulins