Isolation of furocoumarins from bergamot fruits as HL-60 differentiation-inducing compounds.
The HL-60 differentiation-inducing compounds in bergamot fruits were isolated with column chromatography and identified as bergamottin, bergapten, and citropten by (1)H and (13)C NMR. Their HL-60 differentiation-inducing activity was measured by examining nitro blue tetrazolium (NBT) reducing, nonspecific acid esterase (NSE), specific esterase (SE), and phagocytic activities, and bergamottin showed the strongest activity among the coumarins isolated from bergamot fruits. The structure-activity relationship obtained from HL-60 differentiation assay suggests that hydrophobicity of furocoumarins is correlated with their activity.
Kawaii
S
S
National Institute of Fruit Tree Science, Okitsu, Shimizu, Shizuoka 424-0204, Japan.
Tomono
Y
Y
Katase
E
E
Ogawa
K
K
Yano
M
M
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D011564:Furocoumarins