A series of protein phosphatase gene disruptants in Saccharomyces cerevisiae.
Thirty-two protein phosphatase (PPase) genes were identified in the genome nucleotide sequence of Saccharomyces cerevisiae. We constructed S. cerevisiae disruptants for each of the PPase genes and examined their growth under various conditions. The disruptants of six putative PPase genes, i.e. of YBR125c, YCR079w, YIL113w, YJR110w, YNR022c and YOR090c, were created for the first time in this study. The glc7, sit4 and cdc14 disruptants were lethal in our strain background. The remaining 29 PPase gene disruptants were viable at 30 degrees C and 37 degrees C, but only one disruptant, yvh1, showed intrinsic cold-sensitive growth at 13 degrees C. Transcription of the YVH1 gene was induced at 13 degrees C, consistent with an idea that Yvh1p has a specific role for growth at a low temperature. The viable disruptants grew normally on nutrient medium containing sucrose, galactose, maltose or glycerol as carbon sources. The ppz1 disruptant was tolerant to NaCl and LiCl, while the cmp2 disruptant was sensitive to these salts, as reported previously, and none of the other viable PPase disruptants exhibited the salt sensitivity. When the viable disruptants were tested for sensitivity to drugs, i.e. benomyl, caffeine and hydroxyurea, ppz1 and ycr079w disruptants exhibited sensitivity to caffeine.
Sakumoto
N
N
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Mukai
Y
Y
Uchida
K
K
Kouchi
T
T
Kuwajima
J
J
Nakagawa
Y
Y
Sugioka
S
S
Yamamoto
E
E
Furuyama
T
T
Mizubuchi
H
H
Ohsugi
N
N
Sakuno
T
T
Kikuchi
K
K
Matsuoka
I
I
Ogawa
N
N
Kaneko
Y
Y
Harashima
S
S
D001542:Benomyl / Q000494:pharmacology; D015152:Blotting, Northern; D002110:Caffeine / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry; D005659:Fungicides, Industrial / Q000494:pharmacology; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005990:Glycerol / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology; D018021:Lithium Chloride / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D016296:Mutagenesis; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D012965:Sodium Chloride / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D017931:DNA Primers; D004271:DNA, Fungal; D005659:Fungicides, Industrial; D010726:Phosphodiesterase Inhibitors; D002110:Caffeine; D012965:Sodium Chloride; D013395:Sucrose; D008320:Maltose; D010749:Phosphoprotein Phosphatases; D018021:Lithium Chloride; D005990:Glycerol; D001542:Benomyl; D005690:Galactose; D006918:Hydroxyurea