Flow cytometrical determination of interleukin 1beta, interleukin 6 and tumour necrosis factor alpha in monocytes of rheumatoid arthritis patients; relation with parameters of osteoporosis.
Experimental data suggest that pro-inflammatory cytokines such as interleukin 1beta (IL-1beta), interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-alpha) are important in the pathogenesis of osteoporosis in rheumatoid arthritis. Therefore we compared the production of these cytokines by monocytes in 10 rheumatoid arthritis patients and 10 controls. Cytokine levels in rheumatoid arthritis patients were related to disease activity parameters, bone mineral density (BMD) corrected for age and sex (Z scores) and osteocalcin as a laboratory parameter of bone remodelling. Cytokines were determined by a flow cytometrical technique. There was a tendency for higher IL-1beta levels in patients compared with controls. A positive correlation between erythrocyte sedimentation rate and spontaneous production of monocytic cytokines was found. Z scores of the lumbar spine showed a negative correlation with spontaneous production of IL-1beta and IL-6. Plasma osteocalcin levels were positively correlated with spontaneous production of IL-1beta, IL-6 and TNF-alpha. In conclusion, the correlation of the levels of these cytokines with parameters of bone metabolism and osteoporosis suggest that especially IL-1beta and IL-6 are associated with more pronounced osteoporosis in active rheumatoid arthritis.
Verbruggen
A
A
Department of Immunology, Allergology, Rheumatology, University of Antwerp (UIA), Antwerp, Belgium.
De Clerck
L S
LS
Bridts
C H
CH
Van Offel
J F
JF
Stevens
W J
WJ
D015502:Absorptiometry, Photon; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000150:complications / Q000378:metabolism*; D001799:Blood Sedimentation; D015519:Bone Density; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008161:Lumbosacral Region; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D015675:Osteocalcin / Q000097:blood; D010024:Osteoporosis / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism*; D012720:Severity of Illness Index; D013131:Spine / Q000000981:diagnostic imaging; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin