Restriction enzyme analysis of PCR amplified rDNA as a taxonomic tool in yeast identification.
A method has been developed to simplify the identification of yeast strains. We used the restriction fragment patterns of PCR-amplified 18S rRNA-coding DNA with the neighbouring ITS1 region for differentiation and identification of 169 yeast strains representing 128 species associated mainly with food, wine, beer, and soft drinks. The amplicons were digested with four different four-base-cutting restriction enzymes. To construct a database of restriction fragment patterns, the gels have been scanned and analyzed using the Molecular Analyst Fingerprint 2.0 software. The use of four enzymes proved to be sufficient for strain identification.
Dlauchy
D
D
University of Horticulture and Food Science, Budapest, Hungary. dlauchy@hoya.kee.hu
Tornai-Lehoczki
J
J
Péter
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G
D001515:Beer / Q000382:microbiology; D001628:Beverages / Q000382:microbiology; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005516:Food Microbiology; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000032:analysis; D014920:Wine / Q000382:microbiology; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S