A new HPLC method for the direct analysis of triglycerides of dicarboxylic acids in biological samples.
Dicarboxylic acids (DA) are alternate lipid substrates recently proposed in parenteral nutrition. Two new derivatives of DA, a triglyceride of sebacic (TGC10) and one of dodecanedioic (TGC12) acid have been synthesised in order to reduce the amount of sodium given with the unesterified forms. The present paper describes a rapid and direct high-performance liquid chromatographic method (HPLC) for the analysis of these substances in both plasma and urine. Thirty-six male Wistar rats were rapidly injected with 64 mg of TGC10 or 53 mg of TGC12. The triglycerides and their products of hydrolysis were measured in plasma samples taken at different times. For the dose of 500 ng the intra-assay variations ranged from 6. 80+/-0.35% for TGC10 to 18.6+/-3.20% for TGC12 and the inter-assay variations were from 4.44+/-2.21% for TGC10 to 15.0+/-6.72% for TGC12. The detection limit for both triglycerides was 5 ng. This rapid and direct HPLC method could have practical implications in monitoring the concentration of both triglycerides and free forms of DA in biological samples of patients who might benefit from the administration of these substances during parenteral nutrition regimens.
Capristo
E
E
Istituto di Medicina Interna e Geriatria, Università Cattolica S. Cuore, Largo A. Gemelli, 8-00168, Rome, Italy. ecapristo@pelagus.it
Mingrone
G
G
De Gaetano
A
A
Addolorato
G
G
Greco
A V
AV
Gasbarrini
G
G
D000818:Animals; D002138:Calibration; D002210:Caprylates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003652:Decanoic Acids / Q000032:analysis; D003998:Dicarboxylic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000032:analysis* / Q000097:blood / Q000652:urine
D002210:Caprylates; D003652:Decanoic Acids; D003998:Dicarboxylic Acids; D014280:Triglycerides; C005738:suberic acid; C036836:dodecanedioic acid; C011107:sebacic acid; C010038:azelaic acid