D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics.
Three short tandem repeat (STR) polymorphisms characterized by PCR product length < 175 bp were investigated. D18S535 and D1S1656 contained a 4 bp unit as basic repeat motif, D10S2325 a 5 bp unit. The heterozygosity rates were 0.76 (D18S535), 0.88 (D10S2325) and 0. 90 (D1S1656), leading to a combined discrimination power of 0.9999. In contrast to D10S2325 and D18S535, which showed a homogeneous repeat array without any variation in the repeat motifs, repeat length and sequence variation was found for D1S1656. Robust typing results could be observed for all three STRs using highly degraded DNA.
Wiegand
P
P
Institute of Legal Medicine, Martin-Luther-Universität Halle-Wittenberg, Franzosenweg 1, D-06112 Halle/Saale, Germany.
Lareu
M V
MV
Schürenkamp
M
M
Kleiber
M
M
Brinkmann
B
B
D000483:Alleles; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005554:Forensic Medicine; D005828:Genetics, Population; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences