Interaction of chickpea (Cicer arietinum L.) legumin with oxidized linoleic acid.
Chickpea legumin has been purified and incubated under oxidizing conditions with linoleic acid to investigate the influence of this acid on the structure and nutritional quality of the protein. At the end of the incubation time, >30% of the linoleic acid was oxidized. The oxidized linoleic acid was highly detrimental to legumin, and the electrophoretic pattern of the protein was completely changed after the incubation period. Nevertheless, neither polymerization nor cleavage of the protein was observed as deduced from gel filtration chromatography, suggesting that the changes observed in native electrophoresis were probably due to oxidation of legumin. The incubation of legumin with linoleic acid also produced a diminution of the contents of methionine and histidine, by 81.3 and 24.3%, respectively. Finally, in vitro protein digestibility of chickpea legumin was also seriously affected by the incubation with linoleic acid, decreasing from 84.1 to 69.2%.
Sánchez-Vioque
R
R
Departamento de Fisiología y Tecnología de Productos Vegetales, Instituto de la Grasa (CSIC), Avenida Padre García Tejero 4, 41012 Sevilla, Spain.
Vioque
J
J
Clemente
A
A
Pedroche
J
J
Bautista
J
J
Millán
F
F
D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D007887:Fabaceae; D019787:Linoleic Acid / Q000737:chemistry*; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry; D000097793:Legumins
D000596:Amino Acids; D019787:Linoleic Acid; D010940:Plant Proteins; D000097793:Legumins