title: [Determination of the in vitro antifungal susceptibility of clinically important yeasts using the Sensititre system].
abstract: Using Sensititre (AccuMed, USA) we studied the in vitro antifungal activity of amphotericin B, fluconazole, itraconazole, ketoconazole and 5-fluorocytosine against 250 clinical yeast isolates taken from different hospitals, including Candida (151 C. albicans, 15 C. krusei, 14 C. parapsilosis, 11 C. tropicalis, 10 C. glabrata, 4 C. guilliermondii, 3 C. rugosa, 2 C. viswanathii, 2 C. famata and 2 C. kefyr), Cryptococcus (32 C. neoformans and 1 C. laurentii), Trichosporon (2 isolates) and Rhodotorula rubra (1 isolate). All the strains were susceptible to amphotericin B and showed an MIC <1 mg/l. The susceptibility of C. albicans (MIC(90) <256 mg/l), C. krusei (MIC(90) <64 mg/l), C. glabrata (MIC(90) <64 mg/l) and C. neoformans (MIC(90) 32 mg/l) to fluconazole was lower (14% isolates being resistant and 16.8% susceptible depending on the dose). The largest number of strains resistant to itraconazole was observed in C. albicans and C. glabrata (17.2% resistant and 24% susceptible and susceptible depending on the dose, respectively). Ketoconazole and 5-fluorocytosine were not effective in vitro against 12.8% and 2%, respectively, of all the isolates studied. Nine C. krusei and seven C. neoformans (12.9%) showed dose-dependent susceptibility to 5-fluorocytosine.
authors.affiliation: Departamento de Microbiología, ACIA, c/Pintor Casas n(o) 11-15, 08031 Barcelona.
authors.forename: A J
authors.initials: AJ
authors.lastname: Carrillo-Muñoz
authors.forename: G
authors.initials: G
authors.lastname: Quindós
authors.forename: I
authors.initials: I
authors.lastname: Gasser
authors.forename: C
authors.initials: C
authors.lastname: Tur-Tur
authors.forename: M T
authors.initials: MT
authors.lastname: Ruesga
authors.forename: R
authors.initials: R
authors.lastname: Alonso-Vargas
authors.forename: P
authors.initials: P
authors.lastname: Arévalo
authors.forename: F J
authors.initials: FJ
authors.lastname: Bornay-Llinares
authors.forename: P
authors.initials: P
authors.lastname: Santos
authors.forename: O
authors.initials: O
authors.lastname: del Valle
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015003:Yeasts / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole; D007654:Ketoconazole