Existence of IL-8 degrading protease and anti-IL-8 immunoglobulin G in gingival crevicular fluid.
OBJECTIVE
The aim of this study is to identify the nature of the IL-8 inhibitor(s) in gingival crevicular fluid (GCF).


METHODS
Part 1: Fourteen GCF samples were collected from 13 adult periodontitis (AP) patients, and 9 samples were taken from 8 healthy subjects. Each GCF sample was divided into two aliquots. A serine protease-specific inhibitor, PMSF, was added to one aliquot, and PBS was added to the other aliquot as control. ELISA was used to measure IL-8 level in the samples. Part 2: Forty-one GCF samples were collected from 15 AP patients and indirect ELISA was performed to detect the anti-IL-8 IgG antibody.


RESULTS
Part 1: IL-8 level in the PMSF samples was significantly greater than that in the control group (3.01 +/- 5.79 mg/L vs 0.05 +/- 0.15 mg/L, respectively P < .001). Part 2: The mean value of anti-IL-8 IgG in GCF was greater than that of negative control + 3 x SD.


CONCLUSIONS
A serine protease that can "cleave" IL-8 exists in GCF. Gingival crevicular fluid from AP sites contains an autoantibody against IL-8.
Xie
H
H
Department of Periodontology, College and Hospital of Stomatology, Hubei Medical University, P.R. China.
Cao
C F
CF
Ma
D L
DL
Meng
H X
HX
D000328:Adult; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000201:enzymology* / Q000276:immunology*; D011994:Recombinant Proteins; D012697:Serine Endopeptidases / Q000032:analysis* / Q000378:metabolism
D007074:Immunoglobulin G; D016209:Interleukin-8; D011994:Recombinant Proteins; D012697:Serine Endopeptidases