Purification and identification of components of gamma-oryzanol in rice bran Oil.
High-purity gamma-oryzanol was obtained from crude rice bran oil using a normal-phase preparative scale HPLC. A reverse-phase HPLC method was used for separating the individual components of gamma-oryzanol present in rice bran oil. Ten fractions were isolated and collected using the reverse-phase HPLC method, and their structures were identified. Identification was accomplished using GC/MS with an electron impact mass spectrum after components were transformed into trimethylsilyl ether derivatives. The 10 components of gamma-oryzanol were identified as Delta(7)-stigmastenyl ferulate, stigmasteryl ferulate, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, Delta(7)-campestenyl ferulate, campesteryl ferulate, Delta(7)-sitostenyl ferulate, sitosteryl ferulate, compestanyl ferulate, and sitostanyl ferulate. Three of these, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate, were major components of gamma-oryzanol.
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Department of Food Science, Louisiana Agricultural Experimental Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA.
Godber
J S
JS
D002851:Chromatography, High Pressure Liquid; D010666:Phenylpropionates / Q000032:analysis / Q000302:isolation & purification*; D010938:Plant Oils / Q000737:chemistry*; D000073879:Rice Bran Oil
D010666:Phenylpropionates; D010938:Plant Oils; D000073879:Rice Bran Oil; C013172:gamma-oryzanol