title: Characterization of a new variant DNA (cytosine-5)-methyltransferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus.
abstract: The enzyme S-adenosylmethionine-DNA (cytosine-5)-methyltransferase has been identified, first time for invertebrates, in embryos of the marine polychaete annelid worm Chaetopterus variopedatus. The molecule has been isolated from embryos at 15 h of development. It is a single peptide of about 200 kDa molecular weight, cross-reacting with antibodies against sea urchin DNA methyltransferase. The enzymatic properties of the molecule are similar to those of Dnmt1 methyltransferases isolated from other organisms, but with the peculiarity to be unable to make 'de novo' methylation on double stranded DNA.
authors.affiliation: Department of Genetics, General and Molecular Biology, University of Naples Federico II, Via Mezzocannone, 8, 80134, Napoli, Italy.
authors.forename: R
authors.initials: R
authors.lastname: del Gaudio
authors.forename: R
authors.initials: R
authors.lastname: Di Giaimo
authors.forename: N
authors.initials: N
authors.lastname: Potenza
authors.forename: M
authors.initials: M
authors.lastname: Branno
authors.forename: F
authors.initials: F
authors.lastname: Aniello
authors.forename: G
authors.initials: G
authors.lastname: Geraci
mesh_terms: D000818:Animals; D000845:Annelida / Q000201:enzymology*; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry*; D019175:DNA Methylation; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D007700:Kinetics; D016982:Micrococcus luteus / Q000235:genetics; D013997:Time Factors
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases