title: The effect of a re-wetting agent on dentin bonding.
abstract: OBJECTIVE
  Recently, a new generation of simplified one-bottle dentin bonding systems, sensitive to variations in the degree of substrate moisture, was introduced. This in vitro project compared the dentin bond strengths and interfacial ultra-morphology formed by three one-bottle bonding systems [OptiBond SOLO (ethanol-based), Prime&Bond 2.1 (acetone-based), and Single Bond (ethanol- and water-based)]. The null hypothesis tested was that re-wetting a dried dentin surface with a HEMA aqueous solution would not result in bond strengths, and resin impregnation into demineralized dentin, comparable to those obtained for moist dentin.
  
  
  METHODS
  Dentin specimens were assigned to the following three etched surface conditions: moist dentin-control group; dentin dried for 5 s; and dentin dried for 5 s and re-moistened with a commercial 35% HEMA aqueous solution. Mean shear bond strengths were calculated and analyzed with one- and two-way ANOVA. Dentin discs treated with the same combination of surface condition/adhesive were processed and observed under both transmission and scanning electron microscopes.
  
  
  RESULTS
  For moist dentin, the morphology of the resin-dentin interfaces showed penetration of the dentin adhesives to the depth of the transition between demineralized and unaffected dentin. Drying dentin for 5 s resulted in a significant decrease in mean bond strengths and an incompletely infiltrated collagen structure with areas of unveiled collagen fibers, regardless of the solvent. Re-wetting dentin with the aqueous HEMA solution re-established the level of bond strengths obtained to moist dentin and resulted in a raise of the fiber network with simultaneous increase in interfibrillar space dimensions.
  
  
  CONCLUSIONS
  The results suggest that the use of an aqueous HEMA solution might compensate for the dryness induced on dentin surfaces by using air blasts from an air syringe, after rinsing off the etchant. As the behavior of the material that contained water was also affected by surface dryness, the percentage of water included in the composition of current ethanol- and water-based adhesives, such as Single Bond, may not be enough to compensate for the collapse of the collagen filigree upon drying.
authors.affiliation: Department of Operative Dentistry, School of Dentistry, University of North Carolina at Chapel Hill 27599-7450, USA. jorge_perdigao@dentistry.unc.edu
authors.forename: J
authors.initials: J
authors.lastname: Perdigão
authors.forename: B
authors.initials: B
authors.lastname: Van Meerbeek
authors.forename: M M
authors.initials: MM
authors.lastname: Lopes
authors.forename: W W
authors.initials: WW
authors.lastname: Ambrose
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002417:Cattle; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D008854:Microscopy, Electron; D017348:Microscopy, Electron, Scanning Transmission; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D014906:Wetting Agents
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C092987:Optibond; D011109:Polymethacrylic Acids; C103500:Prime & Bond; D019279:Resin Cements; D014906:Wetting Agents; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate