title: Negative control of flagellum synthesis in Pseudomonas aeruginosa is modulated by the alternative sigma factor AlgT (AlgU).
abstract: Many respiratory isolates of Pseudomonas aeruginosa from cystic fibrosis patients are mucoid (alginate producing) yet lack flagella. It was hypothesized that an alginate regulator inhibits flagellar gene expression. Mutations in algB, algR, and algT resulted in nonmucoid derivatives, yet algT mutants expressed flagella. AlgT-dependent control of flagellum synthesis occurred through inhibition of fliC but not rpoN transcription.
authors.affiliation: Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Garrett
authors.forename: D
authors.initials: D
authors.lastname: Perlegas
authors.forename: D J
authors.initials: DJ
authors.lastname: Wozniak
mesh_terms: D000464:Alginates / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002237:Carbohydrate Dehydrogenases / Q000235:genetics; D050557:Catechol 2,3-Dioxygenase; D004268:DNA-Binding Proteins; D049308:Dioxygenases; D005407:Flagella / Q000235:genetics / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008854:Microscopy, Electron; D009068:Movement; D009154:Mutation; D010105:Oxygenases / Q000235:genetics; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012808:Sigma Factor / Q000378:metabolism*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: C110681:AlgR protein, Pseudomonas aeruginosa; C079619:AlgU protein, Pseudomonas aeruginosa; D000464:Alginates; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D015534:Trans-Activators; D014157:Transcription Factors; D005408:Flagellin; C072862:AlgB protein, Pseudomonas; D002237:Carbohydrate Dehydrogenases; C020562:GDPmannose dehydrogenase; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase