title: Effect of citric acid on the nucleation of hydroxyapatite in a simulated body fluid.
abstract: The role of citric acid in a simulated body fluid (SBF) was examined with the main focus on its induction ability of hydroxyapatite (HAp) nucleation on a bioinert collagen membrane. Collagen membranes were soaked in the SBF with citric acid concentrations in the range of 0-4 mM; then, carbonate-containing HAp crystals grew within a limited range, i.e., 0.3-2 mM. The results were explained in terms of chemical interaction among calcium ion, citric acid and collagen membrane, especially strong chelation ability of citric acid with the calcium ion.
authors.affiliation: Korea Research Institute of Chemical Technology, Taejon, South Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Rhee
authors.forename: J
authors.initials: J
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001826:Body Fluids / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D002417:Cattle; D019343:Citric Acid / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D008954:Models, Biological; D008970:Molecular Weight; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D019343:Citric Acid; D003094:Collagen; D017886:Durapatite; D002118:Calcium