Integrin alphaVbeta5 is not involved in adeno-associated virus type 2 (AAV2) infection.
alphaVbeta5 integrin was recently proposed as a coreceptor for adeno-associated virus type 2 (AAV2) infection (Summerford et al., 1999, Nat. Med. 5, 78-82), based mainly on the direct binding of AAV2 to denatured beta5 by virus overlay assay. In studies using purified natural or recombinant human integrin alphaVbeta5 we were unable to demonstrate AAV2 binding, either by virus overlay or by liquid binding assay. Furthermore, neither purified integrin alphaVbeta5, nor RGD peptides, nor functional blocking monoclonal antibody blocked rAAV2 transduction. These data strongly suggest that integrin alphaVbeta5 is not involved in AAV2 infection.
Qiu
J
J
Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland, 20892, USA. QuiJ@nih.gov
Brown
K E
KE
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D000229:Dependovirus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D019030:Receptors, Vitronectin; D000097796:Nucleolin
D016023:Integrins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D019030:Receptors, Vitronectin; D000097796:Nucleolin; C064660:integrin alphaVbeta5