title: Expression and intracellular processing of chimeric and mutant CFTR molecules.
abstract: The cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic AMP-activated chloride channel comprising two membrane-spanning domains (MSDs), two nucleotide-binding domains (NBDs) and a unique regulatory (R) domain. The most frequent cystic fibrosis (CF) mutation, a deletion of Phe508 in NBD1, results in the retention of the DeltaF508 CFTR in the endoplasmic reticulum, as do many other natural or constructed mutations located within the first NBD. In order to further define the role of NBD1 in CFTR folding and to determine whether the higher frequency of mutations in NBD1 with respect to NBD2 results from its position in the molecule or is related to its primary sequence, we constructed and expressed chimeric CFTRs wherein NBD domains were either exchanged or deleted. Synthesis, maturation and activity of the chimeras were assessed by Western blotting and iodide efflux assay after transient or stable expression in COS-1 or CHO cells respectively. The data showed that deletion of NBD1 prevented transport of CFTR to the cytoplasmic membrane whereas deletion of NBD2 did not impair this process but resulted in an inactive chloride channel. On the other hand, substituting or inverting NBDs in the CFTR molecule impaired its processing. In addition, while the NBD1 R555K mutation is known to partially correct the processing of CFTR DeltaF508 and to increase activity of both wild-type and DeltaF508 individual channels, it showed no positive effect when introduced into the double NBD1 chimera. Taken together, these observations suggest that the proper folding process of CFTR results from complex interactions between NBDs and their surrounding domains (MSDs and/or R domain).
authors.affiliation: Applied Genetics, Free University of Brussels, rue des Professeurs Jeener et Brachet 12, B-6041, Gosselies, Belgium. jpollet@sga.ulb.ac.be
authors.forename: J F
authors.initials: JF
authors.lastname: Pollet
authors.forename: J
authors.initials: J
authors.lastname: Van Geffel
authors.forename: E
authors.initials: E
authors.lastname: Van Stevens
authors.forename: R
authors.initials: R
authors.lastname: Van Geffel
authors.forename: R
authors.initials: R
authors.lastname: Beauwens
authors.forename: A
authors.initials: A
authors.lastname: Bollen
authors.forename: P
authors.initials: P
authors.lastname: Jacobs
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis* / Q000235:genetics*; D006031:Glycosylation; D007454:Iodides / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D016297:Mutagenesis, Site-Directed; D017510:Protein Folding; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013329:Structure-Activity Relationship; D014162:Transfection
chemical_list: D007454:Iodides; D011993:Recombinant Fusion Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000242:Cyclic AMP