Effect of glutathione L-cystein and L-djenkolic acid in the synthesis and mutagenicity of azide metabolite in Bacillus subtilis ATCC 6633 strain.
The Bacillus subtilis ATCC 6633 strain synthesizes a mutagenic metabolite from sodium azide and O-acetylserine. Mutagenicity of azide was decreased in growth media containing 10(-4) M glutathione, L-cysteine or L-djenkolic acid whereas dithiothritol (DTT) added at the same concentration did not reduce the mutagenicity of azide. Likewise, glutathione, L-cysteine, L-djenkolic acid, and DTT were found to have no effect in reducing the mutagenicity of the in vitro produced metabolite using bacterial cell-free extract. These results suggest that O-acetyl-serine sulfhydrylase catalyzes the reaction of azide and O-acetylserine to form a mutagenic metabolite, which is ninhydrin positive and migrates in TLC to an Rf value similar to that of azidoalanine in both acidic and basic solvent systems.
Elbetieha
A
A
Department of Biological Sciences, Faculty of Science, Jordan University of Science and Technology, Irbid, Jordan.
Owais
W M
WM
Saadoun
I
I
Hussein
E
E
D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology*; D009153:Mutagens / Q000378:metabolism*; D012694:Serine / Q000031:analogs & derivatives / Q000378:metabolism; D019810:Sodium Azide / Q000737:chemistry / Q000378:metabolism*
D009153:Mutagens; C004851:djenkolic acid; D012694:Serine; D019810:Sodium Azide; C043943:O-acetylserine; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol