title: Purification and some properties of an Aspergillus niger beta-apiosidase from an enzyme preparation hydrolyzing aroma precursors.
abstract: A beta-apiosidase was isolated and purified to electrophoretic homogeneity from an enzyme preparation, Klerzyme 200, through ammonium sulfate precipitation, gel filtration chromatography, ion-exchange chromatography, and HPLC on ion-exchange and size exclusion columns. The purification of the enzyme was aided by the synthesis of 4-methylumbelliferyl beta-D-apiofuranoside for the specific detection of activity on electrophoresis gels. The molecular mass estimated by SDS-PAGE was 120 kDa. The optimum activity of the beta-apiosidase was found at pH 5 and 40 degrees C. The K(m) and V(max) for p-nitrophenyl beta-D-apiofuranoside were 4.2 mM and 2460 nkat/mg of protein, respectively. The enzyme was not inhibited by glucose and ethanol. This enzyme hydrolyzed the intersugar linkages of apiofuranosylglucosides, aroma precursors from grape.
authors.affiliation: Laboratoire des Biopolymères et Arômes and Laboratoire de Microbiologie et Technologie des Fermentations, INRA, IPV, 2 place Viala, 34060 Montpellier, France.
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: J M
authors.initials: JM
authors.lastname: Salmon
authors.forename: R
authors.initials: R
authors.lastname: Baumes
authors.forename: C
authors.initials: C
authors.lastname: Tapiero
authors.forename: Z
authors.initials: Z
authors.lastname: Günata
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005959:Glucosidases / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D009812:Odorants
chemical_list: D005959:Glucosidases; C412565:beta-apiosidase