A new selective medium for Bifidobacterium spp.
A new selective antibiotic-free medium for Bifidobacterium spp. is defined. This medium has lactulose as the main carbon source and includes methylene blue, propionic acid, and lithium chloride as inhibitors of some related bacterial species. The low pH of the medium contributes to the inhibition of the growth of Enterobacteriaceae. This new selective medium has a simple composition, and the level of recovery it yields is similar to those yielded by nonselective media for Bifidobacterium strains. It could thus be used for routine analysis in environmental or food microbiology.
Nebra
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Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, E-08028 Barcelona, Spain. ynebra@porthos.bio.ub.es
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D001431:Bacteriological Techniques; D001644:Bifidobacterium / Q000145:classification / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media; D004755:Enterobacteriaceae / Q000187:drug effects / Q000254:growth & development; D004784:Environmental Monitoring; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D007792:Lactulose / Q000378:metabolism; D018021:Lithium Chloride / Q000494:pharmacology; D008751:Methylene Blue / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology
D003470:Culture Media; D011422:Propionates; D007792:Lactulose; D018021:Lithium Chloride; C029658:propionic acid; D008751:Methylene Blue