title: An improved transposon for the halophilic archaeon Haloarcula hispanica.
abstract: An improved transposon (ThD73) for Haloarcula hispanica is described. Based on the halobacterial insertion sequence ISH28, it showed little target sequence specificity but was biased toward a lower G+C content. Twenty randomly selected ThD73 mutants were analyzed, and the DNA flanking their insertions revealed several recognizable sequences, including two (unrelated) ISH elements.
authors.affiliation: Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia.
authors.forename: W G
authors.initials: WG
authors.lastname: Woods
authors.forename: K
authors.initials: K
authors.lastname: Ngui
authors.forename: M L
authors.initials: ML
authors.lastname: Dyall-Smith
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D019613:Haloarcula / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements