[Experimental study on direct toxicity of combined cisplatin and methotrexate for normal and neoplastic cell lines].
The combination effect of cisplatin (CDDP) and methotrexate (MTX) on the cytotoxicity against normal fibroblast and malignant cell line were investigated utilizing MTT assay. In case of normal fibroblast, the optical density (OD) value in CDDP + MTX was significantly higher and remained at a consistently higher value in various mixture rates than that in CDDP alone. Such low cytotoxicity may be due to MTX because the addition of Leucovorin resulted in an apparent decrease in the OD value. The combination of the two drugs was thought to alleviate the toxicity of CDDP on fibroblast, in contrast to its increase on cancer cells.
Beppu
T
T
First Dept. of Surgery, Kochi Medical School.
Araki
K
K
Okamoto
K
K
Ohmi
K
K
Takano
A
A
Kobayashi
M
M
Matsuura
K
K
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D002945:Cisplatin / Q000009:adverse effects / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D006801:Humans; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002945:Cisplatin; D008727:Methotrexate