Inhibition of lipid peroxidation by Lactobacillus acidophilus and Bifidobacterium longum.
The inhibition of lipid peroxidation by Lactobacillus acidophilus and Bifidobacterium longum was investigated using two lipid model systems. All eight strains, including six strains of L. acidophilus and two strains of B. longum, demonstrated an inhibitory effect on linoleic acid peroxidation. The inhibitory rates on linoleic acid peroxidation ranged from 33 to 46% when 1 mL of intracellular cell-free extract was tested. In the second model system, the cell membrane of osteoblast was used as the source for biological lipid. The results indicated that all strains were able to protect biological lipids from oxidation. The inhibition rates on cell membrane lipid peroxidation ranged from 22 to 37%. The effect of L. acidophilus and B. longum on inhibition of fluorescent tissue pigment accumulation was also obtained for osteoblastic cells. The inhibition rates on fluorescent tissue pigment accumulation ranged from 20 to 39%. The antioxidative effect of each milliliter of intracellular cell-free extract of L. acidophilus and B. longum was equivalent to 104-172 ppm of butylated hydroxytoluene (BHT). These results indicated that all strains demonstrated high antioxidative activity. The scavenging ability of lipid peroxidation products, tert-butyl hydroperoxide and malondialdehyde, was also evaluated. The results showed that L. acidophilus and B. longum were not able to scavenge the tert-butyl hydroperoxide. Nevertheless, malondialdehyde was scavenged well by these strains.
Lin
M Y
MY
Department of Food Science, National Chung Hsing University, 250 Kuokang Road, Taichung 402, Taiwan.
Yen
C L
CL
D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D002478:Cells, Cultured; D016166:Free Radical Scavengers / Q000494:pharmacology; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D010006:Osteoblasts / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D017962:alpha-Linolenic Acid / Q000737:chemistry; D020122:tert-Butylhydroperoxide / Q000032:analysis
D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D017962:alpha-Linolenic Acid; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide