title: Orientation of the transcription preinitiation complex in archaea.
abstract: The basal transcription machinery of Archaea corresponds to the minimal subset of factors required for RNA polymerase II transcription in eukaryotes. Using just two factors, Archaea recruit the RNA polymerase to promoters and define the direction of transcription. Notably, the principal determinant for the orientation of transcription is not the recognition of the TATA box by the TATA-box-binding protein. Instead, transcriptional polarity is governed by the interaction of the archaeal TFIIB homologue with a conserved motif immediately upstream of the TATA box. This interaction yields an archaeal preinitiation complex with the same orientation as the analogous eukaryal complex.
authors.affiliation: The Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom.
authors.forename: S D
authors.initials: SD
authors.lastname: Bell
authors.forename: P L
authors.initials: PL
authors.lastname: Kosa
authors.forename: P B
authors.initials: PB
authors.lastname: Sigler
authors.forename: S P
authors.initials: SP
authors.lastname: Jackson
mesh_terms: D001105:Archaea / Q000235:genetics*; D019843:Archaeal Proteins; D019848:Gene Expression Regulation, Archaeal; D009687:Nuclear Proteins / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D019843:Archaeal Proteins; D009687:Nuclear Proteins; D020648:Peptide Elongation Factor 1; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; C106466:transcription factor B, Archaea