title: Histological effects of iron accumulation on mice liver and spleen after administration of a metallic solution.
abstract: Special attention has been focused on the toxicity of some metallic species released from implanted materials, which accumulate in vital organs over long periods of time. A set of experiments with mice was designed to investigate the individual effects caused by iron in the liver and spleen. Histological features of these organs were evaluated and slight morphological changes were observed during the treatment time suggesting a negative correlation with the duration of the iron treatment. In addition, to associate the histological changes in the organs with iron accumulation an electrochemical method, adsorptive stripping voltammetry, was chosen to quantify the iron levels in these mentioned organs. The accuracy of the proposed method was checked by atomic absorption spectrometry. Both organs showed elevated concentrations of iron, nearly twofold, 7 days after iron administration compared to control organs.
authors.affiliation: Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Portugal. mcsp@fe.up.pt
authors.forename: M C
authors.initials: MC
authors.lastname: Pereira
authors.forename: M L
authors.initials: ML
authors.lastname: Pereira
authors.forename: J P
authors.initials: JP
authors.lastname: Sousa
mesh_terms: D000818:Animals; D007279:Injections, Subcutaneous; D007501:Iron / Q000008:administration & dosage / Q000032:analysis* / Q000633:toxicity*; D007700:Kinetics; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D012996:Solutions; D013154:Spleen / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D014018:Tissue Distribution
chemical_list: D012996:Solutions; D007501:Iron