title: Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoes.
abstract: Rapid evolution of bunyaviruses may occur by RNA segment reassortment between closely related viruses. Reassortment between viruses occurs in dually infected mosquitoes when two different viruses are simultaneously ingested or when the second virus is ingested within 2 days of the first virus. By 3 days after oral infection, interference to superinfection occurs, thus limiting the potential for evolution. Aedes triseriatus mosquitoes can also be transovarially infected (TI+) with LaCrosse (LAC) virus. In these studies, the potential for oral superinfection of TI+ mosquitoes was assessed. Approximately 20% of mosquitoes TI+ with either a temperature-sensitive LAC virus or wild-type (wt) LAC virus became superinfected by ingesting blood meals containing wt LAC or snowshoe hare (SSH) viruses. LAC virus TI+ mosquitoes superinfected with SSH virus were detected by blot hybridization or RT-PCR. Viruses from these mosquitoes were plaque purified and genotyped using RT-PCR. Reassortant genomes were detected in 2.3% of the viruses genotyped, and 4.0% of the genomes tested were diploid for one genome segment.
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Monica K
authors.initials: MK
authors.lastname: Borucki
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Chandler
authors.affiliation: Department of Entomology, North Carolina State University, Raleigh, NC 27965, USA2.
authors.forename: Beulah M
authors.initials: BM
authors.lastname: Parker
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Carol D
authors.initials: CD
authors.lastname: Blair
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Beaty
mesh_terms: D000330:Aedes / Q000254:growth & development / Q000821:virology*; D000818:Animals; D002141:Encephalitis Virus, California / Q000235:genetics* / Q000502:physiology; D005260:Female; D018062:La Crosse virus / Q000235:genetics* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010053:Ovary / Q000821:virology; D016865:Reassortant Viruses / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction