title: Proliferation of bone marrow pro-B cells is dependent on stimulation by the pituitary/thyroid axis.
abstract: The frequency and absolute number of pro-B, pre-B, and B cells in the bone marrow of the hypothyroid strain of mice are significantly reduced compared with those of their normal littermates. To investigate why this is the case, various B cell developmental processes were examined in the thyroid hormone-deficient mice. These studies revealed that the frequency of pro-B cells in the S-G2/M phase of the cell cycle was significantly reduced in hypothyroid mice. That thyroid hormone deficiency was responsible for this proliferation defect was established by demonstrating that treatment of hypothyroid mice with thyroxine resulted in a specific increase in the frequency and total number of cycling pro-B cells. The latter effect was paralleled by increases in the frequency and number of bone marrow B lineage cells. Additional in vitro experiments revealed that at least some thyroid hormone effects were directly mediated on the bone marrow. Taken together, these data demonstrate that thyroid hormones are required for normal B cell production in the bone marrow through regulation of pro-B cell proliferation and establish a role for the pituitary/thyroid axis in B cell development.
authors.affiliation: Department of Pathology and Laboratory Medicine and Jonsson Comprehensive Cancer Center, School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Foster
authors.forename: E
authors.initials: E
authors.lastname: Montecino-Rodriguez
authors.forename: K
authors.initials: K
authors.lastname: Dorshkind
mesh_terms: D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D002455:Cell Division; D019070:Cell Lineage; D002478:Cells, Cultured; D005472:Fluorouracil / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D007037:Hypothyroidism; D007075:Immunoglobulin M / Q000302:isolation & purification; D017493:Leukocyte Common Antigens; D051379:Mice; D008817:Mice, Mutant Strains; D010902:Pituitary Gland / Q000502:physiology*; D011947:Receptors, Antigen, B-Cell / Q000302:isolation & purification; D013961:Thyroid Gland / Q000502:physiology*; D013963:Thyroid Hormones / Q000172:deficiency / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D013963:Thyroid Hormones; D014284:Triiodothyronine; D017493:Leukocyte Common Antigens; D005472:Fluorouracil