Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components.
Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.
Takeuchi
O
O
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Japan.
Hoshino
K
K
Kawai
T
T
Sanjo
H
H
Takada
H
H
Ogawa
T
T
Takeda
K
K
Akira
S
S
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology*; D003353:Corynebacterium diphtheriae / Q000737:chemistry / Q000276:immunology; D029721:Drosophila Proteins; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology; D005260:Female; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000276:immunology; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000276:immunology; D053592:Interleukin-1 Receptor-Associated Kinases; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D009615:Nocardia / Q000737:chemistry / Q000276:immunology; D010457:Peptidoglycan / Q000276:immunology*; D020033:Protein Isoforms / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D012475:Salmonella / Q000737:chemistry / Q000276:immunology; D015398:Signal Transduction; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000737:chemistry / Q000276:immunology; D013682:Teichoic Acids / Q000276:immunology*; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors
D000942:Antigens, Bacterial; D029721:Drosophila Proteins; D008050:Lipid A; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D010457:Peptidoglycan; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D013682:Teichoic Acids; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; C009900:lipoteichoic acid; D011494:Protein Kinases; D053592:Interleukin-1 Receptor-Associated Kinases