Subcellular trafficking abnormalities of a prion protein with a disrupted disulfide loop.
The single disulfide loop (Cys178-Cys213) of the prion protein (PrP) may stabilize the conformation of this protein by bridging the C-terminal alpha-helices. The substitution mutant Cys178Ala fails to form the prion isoform PrPSc when expressed in scrapie-infected neuroblastoma ScN2a cells (Muramoto et al., Proc. Natl. Acad. Sci. USA 93, 15457-15462). To investigate the reasons for this failure, we introduced the C178A substitution in the full length mouse PrP gene as well as in its N-terminally truncated delta23-88 version. The resulting mutants (C178A and deltaC178A, respectively) were transiently expressed in N2a and CHO cells. Wild-type PrP, wild-type delta23-88 and the point mutant E199K served as controls in these experiments. Compared to the wild-type controls, the C178A mutants were markedly resistant to proteolysis and they were also vastly insoluble in sarcosyl. Studying the metabolic fate of the C178A mutants, we found that in contrast to control PrP molecules, these mutants (i) remained sensitive to the diagnostic endoglycosidase EndoH, (ii) failed to reach the cell surface and (iii) congregated in large juxtanuclear spots. We surmise that these severe trafficking abnormalities may contribute both to the spontaneous aggregation of the C178A mutants and to their reported inability to form PrP(Sc).
Yanai
A
A
Department of Molecular Biology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Meiner
Z
Z
Gahali
I
I
Gabizon
R
R
Taraboulos
A
A
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004220:Disulfides; D019286:Endopeptidase K / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006596:Hexosaminidases / Q000378:metabolism; D051379:Mice; D009154:Mutation; D009447:Neuroblastoma; D018621:PrPC Proteins / Q000235:genetics* / Q000378:metabolism; D018620:PrPSc Proteins / Q000235:genetics*; D011487:Protein Conformation; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D012608:Scrapie; D012995:Solubility; D014407:Tumor Cells, Cultured
D004220:Disulfides; D018621:PrPC Proteins; D018620:PrPSc Proteins; C025231:sarkosyl; D006596:Hexosaminidases; D019286:Endopeptidase K; D012521:Sarcosine