Organization and sequences of p-hydroxybenzaldehyde dehydrogenase and other plasmid-encoded genes for early enzymes of the p-cresol degradative pathway in Pseudomonas putida NCIMB 9866 and 9869.
The gene (designated pchA) encoding the aldehyde dehydrogenase that is required to metabolise the p-hydroxybenzaldehyde produced by the degradation of p-cresol in Pseudomonas putida NCIMB 9866 and 9869 has been identified on plasmids pRA4000 and pRA500, respectively. The gene lies immediately upstream of the pchC and pchF genes encoding the subunits of p-cresol methylhydroxylase (PCMH), the preceeding enzyme in the p-cresol degradative pathway. In pRA500 the latter genes are followed by the genes encoding the alpha (pcaG) and beta (pcaH) subunits of protocatechuate-3,4-dioxygenase, whereas in pRA4000 the genes encoding PCMH are followed by an open reading frame encoding a protein that is similar to the maturase-related protein of P. alcaligenes. A gene, designated pchX, that encodes a protein of unknown function was identified between the pchC and pchF genes in both plasmids.
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Molecular Biology Division, Department of Veterans Affairs Medical Center, San Francisco, CA 94121, USA.
Kim
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Fuller
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Zhang
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McIntire
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D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003408:Cresols / Q000378:metabolism*; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D019751:Intramolecular Transferases; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D003408:Cresols; D004798:Enzymes; C103415:PchC protein, Pseudomonas aeruginosa; C402805:PchX protein, Pseudomonas putida; D011994:Recombinant Proteins; D009249:NADP; D000445:Aldehyde Oxidoreductases; C068611:4-hydroxybenzaldehyde dehydrogenase; D019751:Intramolecular Transferases; C097120:PchA protein, Pseudomonas aeruginosa; C077977:cresol