Distribution of thyrotropin releasing hormone receptor type 2 in rats: an immunohistochemical study.
OBJECTIVE
To investigate the organ distribution of thyrotropin releasing hormone receptor (TRHR) type 2 in rats by immunohistochemical method.


METHODS
TRHR type 2 was identified immunohistochemically in the rat tissues using specific anti-TRHR antiserum raised in New Zealand white rabbits immunized with a conjugate of synthetic TRHR type 2 (5-23) with bovine serum albumin. Immunohistochemical analysis was performed by avidin-biotin complex method.


RESULTS
TRHR type 2 immunoreactivity was visualized in the central nervous system, anterior pituitary, gastric mucosa, Auerbach's and Meissner's nervous branch of the stomach, small intestine and colon, retina amd testis. Significant stain was detected in neural perikarya, axons and dendrites. When using antiserum preincubated with synthetic TRHR type 2(5-23) or anterior pituitary homogenates, no significant stain of anterior pituitary was detected.


CONCLUSIONS
These findings suggest that TRHR type 2 is widely distributed and that the method used is valuable in studying the distribution of TRHR type 2 in rats.
Mitsuma
T
T
Fourth Department of Internal Medicine,Aichi Medical University, Nagakute, Aichi, 480-1195 Japan.
Rhue
N
N
Kayama
M
M
Mori
Y
Y
Yokoi
Y
Y
Adachi
K
K
Ikai
R
R
Nakamura
A
A
Nakayashiki
A
A
Nogimori
T
T
Sakai
J
J
Hirooka
Y
Y
D000818:Animals; D001360:Avidin; D001369:Axons / Q000737:chemistry; D001710:Biotin; D001923:Brain Chemistry; D003106:Colon / Q000294:innervation; D003712:Dendrites / Q000737:chemistry; D005753:Gastric Mucosa / Q000737:chemistry; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000294:innervation; D008297:Male; D009928:Organ Specificity; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000032:analysis*; D012160:Retina / Q000737:chemistry; D013116:Spinal Cord / Q000737:chemistry; D013270:Stomach / Q000294:innervation; D013737:Testis / Q000294:innervation
D018025:Receptors, Thyrotropin-Releasing Hormone; D001360:Avidin; D001710:Biotin