title: Endothelial dysfunction during acute methionine load in hyperhomocysteinaemic patients.
abstract: Hyperhomocysteinaemia has been associated with arterial and venous thrombosis possibly by causing damage to the endothelium. We hypothesised that an oral load in methionine, that increases plasma homocysteine, would also result in an increase in biological markers of endothelial or platelet dysfunction. Then we investigated two groups of patients with arterial or venous occlusive disease: 17 with hyperhomocysteinemia and 12 without hyperhomocysteinemia. We measured in both groups plasma soluble thrombomodulin, von Willebrand factor, P-selectin and tissue factor plasma inhibitor before and 6 hours after a load with 100 mg/kg oral methionine. Methionine load resulted in a significant increase in von Willebrand factor in both groups (P<0.02), suggesting that endothelial dysfunction occurs during the load.
authors.affiliation: Service de Médecine Interne et Pathologie Vasculaire, Hôpital Saint-André, 33075, Bordeaux, France. joel.constans@chu-aquitaine.fr
authors.forename: J
authors.initials: J
authors.lastname: Constans
authors.forename: A D
authors.initials: AD
authors.lastname: Blann
authors.forename: F
authors.initials: F
authors.lastname: Resplandy
authors.forename: F
authors.initials: F
authors.lastname: Parrot
authors.forename: M
authors.initials: M
authors.lastname: Seigneur
authors.forename: M
authors.initials: M
authors.lastname: Renard
authors.forename: J
authors.initials: J
authors.lastname: Amiral
authors.forename: V
authors.initials: V
authors.lastname: Guérin
authors.forename: M R
authors.initials: MR
authors.lastname: Boisseau
authors.forename: C
authors.initials: C
authors.lastname: Conri
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000368:Aged; D001161:Arteriosclerosis / Q000209:etiology* / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D020138:Hyperhomocysteinemia / Q000150:complications* / Q000175:diagnosis; D008297:Male; D008715:Methionine / Q000008:administration & dosage; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D018180:Thrombomodulin / Q000097:blood; D014841:von Willebrand Factor / Q000032:analysis / Q000187:drug effects
chemical_list: D015415:Biomarkers; D019007:P-Selectin; D018180:Thrombomodulin; D014841:von Willebrand Factor; D008715:Methionine