[The cDNA clone and sequence analysis of the coding region of human augmenter of liver regeneration (hALR) gene].
OBJECTIVE
To obtaining cDNA clone of the coding region of human augmenter of liver regeneration(hALR) as a basis for further study.


METHODS
Total RNA of human fetal liver was extracted as a template. First strand of cDNA was synthesised by reverse transcription with olig(dT)-primer and then amplified by PCR with the primers designed by ourself. The size of PCR product was about 380 bp. it was subcloned into pUC19 and was sequenced by auto-sequencing instrument. The sequence of hALR was analysed with PCGENE program.


RESULTS
The cDNA of integrity coding region of hALR was obtained. Its size was 378 bp. The sequence homology of our hALR compared with rat ALR and human ALR recently reported were 86.5% and 99.2%, respectively.


CONCLUSIONS
It was also suggested that hALR takes part in growth regulation of fetal liver during human fetus late development.
Liu
Q
Q
Institute of Viral Hepatitis, Chongqing University of Medical Sciences.
Wang
Z
Z
Luo
Y
Y
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008115:Liver Regeneration / Q000187:drug effects*; D008969:Molecular Sequence Data; D051381:Rats
D018076:DNA, Complementary; D006133:Growth Substances