title: Regions in vertebrate photoreceptor guanylyl cyclase ROS-GC1 involved in Ca(2+)-dependent regulation by guanylyl cyclase-activating protein GCAP-1.
abstract: The membrane bound guanylyl cyclase (GC) photoreceptor membrane GC1 (ROS-GCI) of photoreceptor cells synthesizes cGMP, the intracellular transmitter of vertebrate phototransduction. The activity of ROS-GCI is controlled by small Ca(2+)-binding proteins, named GC-activating proteins (GCAPs). We identified and characterized two short regulatory regions (M445-L456 and L503-1522) in the juxtamembrane domain (JMD) of ROS-GC1 by peptide competition and mutagenesis studies. Both regions are critical for the activation of ROS-GCI by GCAP-1.
authors.affiliation: Institut für Biologische Informationsverarbeitung-1, Forschungszentrum Jülich, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Lange
authors.forename: T
authors.initials: T
authors.lastname: Duda
authors.forename: M
authors.initials: M
authors.lastname: Beyermann
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.forename: K W
authors.initials: KW
authors.lastname: Koch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D051604:Guanylate Cyclase-Activating Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism*; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins
chemical_list: D002135:Calcium-Binding Proteins; D051604:Guanylate Cyclase-Activating Proteins; D019151:Peptide Library; D010455:Peptides; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C110715:guanylate cyclase 1; D006162:Guanylate Cyclase; D002118:Calcium