Compartive study of volatile components and fatty acids of plants and in vitro cultures of parsley (Petroselinum crispum (Mill) nym ex hill).
Volatile compounds from plants, callus tissue cultures, and cell suspensions of parsley (Petroselinum crispum) were captured during the growth cycle using a dynamic headspace extraction and were identified by gas chromatography-mass spectrometry. Parsley plants were found to produce mainly monoterpenes, and the compound of major abundance was p-1,3,8-menthatriene, followed by beta-phellandrene and apiole. Callus cultures and cell suspensions produced aldehydes (nonanal and decanal) that were also detected in parsley plant. The former also produced limonene, acetophenone, and benzotiazol; these were not observed in the plants. The production of volatiles in plants, callus tissue, and cell suspensions was found to be time-dependent. Free and bound fatty acids were also monitored by an in situ method. Palmitic (C16:0) and stearic (C18:0) acids were the most abundant fatty acids in all materials; however, higher levels were found in plants. On the other hand, the unsaturated C16:1 and C16:3 were not detected in the in vitro cultures.
López
M G
MG
Unidad de Biotecnología e Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-Unidad Irapuato), Irapuato, Gto., Mexico. mlopez@irapuato.ira.cinvestav.mx
Sánchez-Mendoza
I R
IR
Ochoa-Alejo
N
N
D000447:Aldehydes / Q000032:analysis; D019661:Apiaceae / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009924:Organ Culture Techniques; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D013729:Terpenes / Q000032:analysis*; D014835:Volatilization
D000447:Aldehydes; D005227:Fatty Acids; D013729:Terpenes