title: Reduction of adhesion formation with cross-linked hyaluronic acid after peritoneal surgery in rats.
abstract: OBJECTIVE
  To examine the effectiveness of a cross-linked hyaluronan solution (auto-cross-linked polysaccharide [ACP] gel) for the prevention of postsurgical adhesions.
  
  
  METHODS
  A randomized blinded study using a rat model of laparotomy.
  
  
  METHODS
  Surgical Research Laboratory in a university medical school.
  
  
  METHODS
  Sixty-seven sexually mature rats.
  
  
  METHODS
  Standardized surgical trauma was induced in the rat uterine horn to induce adhesion formation. After trauma, group-1 animals (n = 23) received no treatment, group 2 (n = 21) received noncross-linked hyaluronic acid (HA), and group 3 (n = 23) received cross-linked HA applied on the lesion.
  
  
  METHODS
  Six weeks after laparotomy, repeat laparotomy was performed and the adhesions were scored according to Blauer's scoring system.
  
  
  RESULTS
  Overall, 84% of the untreated animals and 65% of the animals treated with noncross-linked HA presented with severe adhesions. The mean (+/-SEM) increase in the adhesion score was 2.46+/-0.23 in the untreated group, 2.23+/-0.29 in the group receiving noncross-linked HA, and 1.27+/-0.12 in the ACP gel group.
  
  
  CONCLUSIONS
  ACP gel holds promise as a novel resorbable biomaterial for the reduction of postoperative adhesions after laparotomy.
authors.affiliation: Department of Obstetrics and Gynecology, Ondokuz Mayis University School of Medicine, Samsun, Turkey. kocak@iname.com
authors.forename: I
authors.initials: I
authors.lastname: Koçak
authors.forename: C
authors.initials: C
authors.lastname: Unlü
authors.forename: Y
authors.initials: Y
authors.lastname: Akçan
authors.forename: K
authors.initials: K
authors.lastname: Yakin
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents; D005260:Female; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D007813:Laparotomy / Q000009:adverse effects*; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D000267:Tissue Adhesions / Q000517:prevention & control*
chemical_list: D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid