Kinetics of activation of latent mushroom (Agaricus bisporus) tyrosinase by benzyl alcohol.
A latent isoform of Agaricus bisporus tyrosinase has been isolated and activated by benzyl alcohol, one of the major volatile compounds in mushrooms of this genus. The progress curve that describes the activation process reached the steady-state rate (V(ss)) after a lag period (tau). The rate of active tyrosinase formation was calculated by coupling the oxidation of o-diphenols to the activation process. V(ss) depended on benzyl alcohol, o-diphenol, and latent tyrosinase concentrations. The lag period depended on benzyl alcohol concentrations but not on o-diphenol and enzyme concentrations. The size of the latent mushroom tyrosinase was 67 kDa, determined by SDS-PAGE and Western blotting assays. This size was not modified after activation by benzyl alcohol. The presence of a lag period and the lack of change of the molecular mass of the protein after activation could indicate a slow conformational change of the protein to render the final active form. The values of the kinetic constants V(max) and K(m) on the o-diphenols 4-tert-butylcatechol, L-DOPA, and dopamine were different between the latent tyrosinase activated by benzyl alcohol and the commercial tyrosinase. They might indicate that a different final active tyrosinase, depending on the activator used, could arise.
Espín
J C
JC
Agrotechnological Research Institute (ATO-DLO), Bornsesteeg 59, P.O. Box 17, 6700 AA Wageningen, The Netherlands. J.C.ESPIN@ato.dlo.nl
Wichers
H J
HJ
D000363:Agaricales / Q000201:enzymology*; D000364:Agaricus / Q000201:enzymology*; D019905:Benzyl Alcohol / Q000494:pharmacology*; D004789:Enzyme Activation; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000378:metabolism*
D014442:Monophenol Monooxygenase; D019905:Benzyl Alcohol