BH-protocadherin-c, a member of the cadherin superfamily, interacts with protein phosphatase 1 alpha through its intracellular domain.
Using a yeast two-hybrid system, we isolated eight cDNA clones which interacted with BH-protocadherin-c (BH-Pcdh-c) from the human brain cDNA library. One clone encoded protein phosphatase type I isoform alpha (PP1alpha) and another two PP1alpha2. PP1alpha was co-immunoprecipitated from the extract of a gastric adenocarcinoma cell line MKN-28 with anti-BH-Pcdh-c antibody. PP1alpha activity towards glycogen phosphorylase was inhibited by the intracellular domain of BH-Pcdh-c. Inhibition of the phosphatase required more than the minimal domain of BH-Pcdh-c which could associate with PP1alpha. In situ hybridization revealed that BH-Pcdh-c mRNA was predominantly expressed in cerebral cortex neurons in the adult mouse brain.
Yoshida
K
K
Department of Virology, Institute of Medical Science, The University of Tokyo, Japan.
Watanabe
M
M
Kato
H
H
Dutta
A
A
Sugano
S
S
D000818:Animals; D001921:Brain / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003001:Cloning, Molecular; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D054645:Protein Phosphatase 1; D000091363:Protocadherins; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015003:Yeasts
D015820:Cadherins; D016376:Oligonucleotides, Antisense; C113391:PCDH7 protein, human; D000091363:Protocadherins; D012333:RNA, Messenger; D006005:Phosphorylases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1