[Preparative isolation of gonadotropin and urokinase on carboxylic cation exchangers].
Low-pressure ion-exchange chromatography was used for sequential isolation of chorionic gonadotropin and urokinase (EC 3.4.99.26) from urine of pregnant women. Theoretical analysis of electrostatic interactions of protein macromolecules with the sorptive surface of a carboxylic cation exchanger was performed. This analysis allowed us to optimize the pH values for selective sorption and reversible desorption of the target component on a cation exchanger with a selected acidity of functional groups. The use of carboxylic cation exchangers KM-2p and Biokarb-GM allowed us to obtain the preparations with purity not inferior to commercially available preparations.
Demin
A A
AA
Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia.
Chernova
I A
IA
Potapenko
B E
BE
Toporkova
E B
EB
Shataeva
L K
LK
D000818:Animals; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange; D005260:Female; D006062:Gonadotropins / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D011247:Pregnancy; D051381:Rats; D014568:Urokinase-Type Plasminogen Activator / Q000302:isolation & purification* / Q000652:urine
D002411:Cation Exchange Resins; D006062:Gonadotropins; D014568:Urokinase-Type Plasminogen Activator