Hypertension in unilaterally nephrectomized rats induced by single-kidney transfection with angiotensinogen cDNA.
Plasmid expression vectors containing angiotensinogen (ATG) cDNA were complexed to cationic liposomes and injected into the renal artery of unilaterally nephrectomized rats to evaluate the effect of intrarenal ATG cDNA on arterial blood pressure and the renin-angiotensin system. Systolic blood pressures measured by tail cuff on days 12, 16, and 18 after transfection were significantly higher in rats that received ATG cDNA than in control rats that received the lac Z reporter gene. Plasma renin activity and plasma ATG concentration were unchanged. These results provide direct evidence that the availability of intrarenal ATG may be instrumental in the development of systemic hypertension.
Marley
W S
WS
Department of Physiology, Colorado State University, Fort Collins, CO 80523-1672, USA.
Bowen
R
R
Gotshall
R W
RW
Wilke
L
L
D000808:Angiotensinogen / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D006339:Heart Rate / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology; D006977:Hypertension, Renal / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D007668:Kidney / Q000473:pathology; D008081:Liposomes; D008297:Male; D009392:Nephrectomy; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000378:metabolism; D014162:Transfection / Q000502:physiology*; D019076:Transgenes / Q000235:genetics
D018076:DNA, Complementary; D008081:Liposomes; D000808:Angiotensinogen; D012083:Renin