Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses.
A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.
Williams
K
K
Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, Maine 04469, USA.
Blake
S
S
Sweeney
A
A
Singer
J T
JT
Nicholson
B L
BL
D000818:Animals; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology; D005399:Fishes; D018061:Infectious pancreatic necrosis virus / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012209:Rhabdoviridae / Q000235:genetics / Q000302:isolation & purification; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D014776:Virus Cultivation