PCR-RFLP analysis of mitochondrial DNA: a reliable method for species identification.
A method for identification of game species has been developed on the basis of the amplification of a specific part of the mitochondrial genome (tRNA(Glu)/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several game species, the obtained 464-bp-long PCR products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphism (RFLP). Even closely related deer species could be distinguished by application of one or two RE. Natural polymorphisms of the target sequence within one species were examined for red deer (Cervus elaphus), and base pair substitutions were identified affecting the RFLP pattern.
Wolf
C
C
Laboratory of Food Chemistry and Department of Chemistry and Biochemistry, University of Berne, Switzerland. christian.wolf@ibc.unibe.ch
Rentsch
J
J
Hübner
P
P
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016384:Consensus Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003670:Deer / Q000145:classification / Q000235:genetics*; D006041:Goats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012352:RNA, Transfer, Glu / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D013552:Swine
D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D012352:RNA, Transfer, Glu