title: Eosinophil activation and in situ interleukin-5 production by mononuclear cells in skin lesions of patients with drug hypersensitivity.
abstract: In order to determine the inflammatory mechanisms of skin lesions in patients with drug hypersentivity, we examined eosinophil activation and interleukin-5 (IL-5) production in infiltrating lymphocytes. First, we showed that the number of peripheral eosinophils and the level of serum IL-5 at the eruption-active stage were both significantly higher than those in healed skin eruptions. Histological and immunohistological examination revealed that CD4+ T cells and eosinophils significantly more densely infiltrated drug eruptions than control skin lesions. The infiltrating eosinophils were also shown to be activated by immunostaining using anti-secreted formed eosinophilic cationic protein monoclonal antibody. The expression of mRNA for IL-5 in the infiltrating mononuclear cells at drug eruptions was shown by in situ hybridization. These results suggest that infiltrating CD4+ T cells might regulate both peripheral and tissue eosinophils and facilitate allergic inflammation at drug eruptions by means of IL-5 production.
authors.affiliation: Department of Dermatology, Toho University School of Medicine, Sakura Hospital, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Mikami
authors.forename: K
authors.initials: K
authors.lastname: Ochiai
authors.forename: K
authors.initials: K
authors.lastname: Umemiya
authors.forename: R
authors.initials: R
authors.lastname: Matsumura
authors.forename: M
authors.initials: M
authors.lastname: Kagami
authors.forename: H
authors.initials: H
authors.lastname: Tomioka
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: E
authors.initials: E
authors.lastname: Tanabe
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D016516:CD4-CD8 Ratio; D003875:Drug Eruptions / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D004802:Eosinophilia / Q000139:chemically induced* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D015848:Interleukin-5