Identification and characterization of a transcriptional silencer upstream of the human BRCA2 gene.
Expression of the breast cancer susceptibility tumor-suppressor protein BRCA2, a protein potentially involved in DNA recombination repair, is tightly regulated throughout development. We have identified a transcriptional silencer at the distal end of the human BRCA2 gene promoter. This silencer is involved in the negative regulation of the expression of this gene in breast cell lines tested but not in HeLa or HepG2 cells. The 221-base-pair silencer region is characterized by a full-length Alu-repeat. Presence of specific BRCA2 silencer-binding proteins in the breast cell extracts indicates the potential regulation of BRCA2 gene expression by these proteins.
Sharan
C
C
Division of Biomedical Sciences, Department of Microbiology, Meharry Medical College, Nashville, Tennessee, 37208, USA.
Hamilton
N M
NM
Parl
A K
AK
Singh
P K
PK
Chaudhuri
G
G
D020087:Alu Elements; D000595:Amino Acid Sequence; D024682:BRCA2 Protein; D001483:Base Sequence; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D013094:Spermatozoa; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D024682:BRCA2 Protein; D017931:DNA Primers; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D014157:Transcription Factors; C094973:sperm Alu-binding protein, human; D004247:DNA