title: Nitrite plasma levels after an oral fat meal in normolipemic subjects.
abstract: BACKGROUND
  The authors, investigating the effects of an oral triglyceride-rich fatty load upon the endothelial function as regards the production of nitric oxide, performed the determination of plasma nitrites, which are stable, specific and irreversible end-products of nitric oxide.
  
  
  METHODS
  The series consists of 13 metabolically normal female subjects (mean age 55 +/- 7 years); after an overnight fasting each subject undertook an oral fat load (butter 1 g/kg); a venous blood withdrawal was carried out before oral fat meal and after two and four hours. For each plasma sample total cholesterol, HDL-cholesterol and triglyceride plasma levels were determined by enzymatic methods; LDL-cholesterol was calculated by Friedwald's formula; the nitrite plasma levels were obtained by the Gutman and Hollywood colorimetric method.
  
  
  RESULTS
  Total cholesterol, HDL-cholesterol, LDL-cholesterol did not show significant changes after the oral fat load; triglycerides rose significantly (p < 0.05) after 4 hours as compared to the basal value (226 +/- 12 vs 175 +/- 12 mg/dl, +30%). The nitrite plasma levels were almost unchanged before oral fat load and after 2 and 4 hours.
  
  
  CONCLUSIONS
  The results suggest that the acute biochemical stress consisting of increased triglyceride-rich very low density lipoproteins was not able to stimulate the endothelial production of nitric oxide.
authors.affiliation: Chair of Semeiology and Medical Methodology, University of Catania, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Ferlito
authors.forename: M
authors.initials: M
authors.lastname: Gallina
mesh_terms: D002079:Butter; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D008875:Middle Aged; D009573:Nitrites / Q000097:blood*; D012016:Reference Values
chemical_list: D004041:Dietary Fats; D008055:Lipids; D009573:Nitrites; D002079:Butter