The murine gammaherpesvirus-68 M11 protein inhibits Fas- and TNF-induced apoptosis.
The murine gammaherpesvirus-68 (MHV-68) M11 gene encodes a protein predicted to have limited homology to the bcl-2 family of proteins. Unlike most of the other viral bcl-2 homologues, which have both BH1 and BH2 domains conserved with respect to bcl-2, the M11 protein has a BH1 domain, but apparently lacks a BH2 domain. Transfection of HeLa cells with an epitope-tagged MHV-68 M11 construct showed that the protein is predominantly located in the cytoplasm of cells. In HeLa cells, M11 inhibited apoptosis induced by anti-Fas antibody and by TNF-alpha. Thus, despite its limited conservation with respect to other bcl-2 family members, the MHV-68 M11 protein is a potent inhibitor of apoptosis.
Wang
Guang-Hua
GH
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA1.
Garvey
Tara L
TL
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA1.
Cohen
Jeffrey I
JI
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA1.
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D018142:Gammaherpesvirinae; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D051020:bcl-X Protein; D019014:fas Receptor / Q000378:metabolism*
C494811:BCL2L1 protein, human; C494812:Bcl2l1 protein, mouse; C118862:M3 protein, Murine gammaherpesvirus; D019253:Proto-Oncogene Proteins c-bcl-2; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; D051020:bcl-X Protein; D019014:fas Receptor