Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2.
Nucleotide sequence analysis is increasingly being used to identify bacteria. In this work, a PCR assay based on degenerate primers was used to obtain the partial sequence of infB, the gene encoding translation initiation factor 2 (IF2), in 39 clinical isolates of different Enterobacteriaceae. The partial sequence encodes the GTP-binding domain of IF2. Together with sequences from the literature, a total of 15 species, each represented by one to seven strains, was investigated. Phylogenetic analysis yielded an evolutionary tree which had a topology similar to a tree constructed using available 16S rRNA sequences. It is concluded that the inter-species variation of the infB gene fragment is sufficient for its use in the characterization of strains that have aberrant phenotypic reactions.
Hedegaard
J
J
Department of Molecular and Structural Biology, Aarhus University, Denmark.
Steffensen
S A
SA
Nørskov-Lauritsen
N
N
Mortensen
K K
KK
Sperling-Petersen
H U
HU
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D039881:Prokaryotic Initiation Factor-2; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D010448:Peptide Initiation Factors; D039881:Prokaryotic Initiation Factor-2