title: Tetraspanins expressed in the embryonic chick nervous system.
abstract: Proteins of the tetraspanin superfamily participate in the formation of plasma membrane signaling complexes; recent evidence implicates neuronal tetraspanins in axon growth and target recognition. We used a degenerate PCR screen to identify cDNAs encoding tetraspanins expressed in the embryonic spinal cord. Two cDNAs identified apparently represent chick homologues of NAG-2 (cnag) and CD9 (chCD9). A third clone encodes a novel tetraspanin (neurospanin). All three mRNAs are widely expressed but exhibit developmentally distinct patterns of expression in the nervous system. Both neurospanin and cnag exhibit high relative expression in nervous tissue, including brain, spinal cord and dorsal root ganglia (DRG).
authors.affiliation: Department of Molecular and Cellular Pharmacology, R-189, University of Miami School of Medicine, Miami, FL 33136, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Perron
authors.forename: J L
authors.initials: JL
authors.lastname: Bixby
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology*; D002642:Chick Embryo; D003001:Cloning, Molecular; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000196:embryology*; D060245:Tetraspanin 29; D059470:Tetraspanins; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D015703:Antigens, CD; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C109139:TSPAN4 protein, human; D060245:Tetraspanin 29; D059470:Tetraspanins; C401301:neurospanin