In situ hybridization-theory and practice.
In situ hybridization is a technique to determine and localize target nucleic acids in morphologically preserved tissue sections. Recent advances in methods have greatly increased the sensitivity of the technique, and it is currently possible to detect extremely few copies of any given target sequence with nonisotopic methods. In this teaching review, we integrate theoretical background, technical considerations, and guidelines for usage for this important component of molecular diagnosis.
Kadkol
S S
SS
Department of Pathology, Division of Molecular Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Gage
W R
WR
Pasternack
G R
GR
D000818:Animals; D001345:Autoradiography; D019921:Biotinylation; D002021:Buffers; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004076:Digoxigenin; D005404:Fixatives / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005557:Formaldehyde / Q000494:pharmacology; D006735:Horseradish Peroxidase; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007553:Isotope Labeling; D008867:Microtomy; D015345:Oligonucleotide Probes; D012313:RNA / Q000032:analysis; D015347:RNA Probes; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013194:Staining and Labeling / Q000379:methods; D019809:Streptavidin; D016707:Tissue Fixation / Q000379:methods
D002021:Buffers; D015342:DNA Probes; D005404:Fixatives; D015345:Oligonucleotide Probes; D015347:RNA Probes; D005557:Formaldehyde; D012313:RNA; D004247:DNA; D019809:Streptavidin; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate; D004076:Digoxigenin