Disulfide cross-linking of subunits F(1)-gamma and F(0)I-PVP(b) results in asymmetric effects on proton translocation in the mitochondrial ATP synthase.
A study is presented on the effect of diamide-induced disulfide cross-linking of F(1)-gamma and F(0)I-PVP(b) subunits on proton translocation in the mitochondrial ATP synthase. The results show that, upon cross-linking of these subunits, whilst proton translocation from the A side to the B F(1) side is markedly accelerated with decoupling of oxidative phosphorylation, proton translocation in the reverse direction, driven by either ATP hydrolysis or a diffusion potential, is unaffected. These observations reveal further peculiarities of the mechanism of energy transfer in the ATP synthase of coupling membranes.
Gaballo
A
A
Department of Medical Biochemistry and Biology, University of Bari, Piazza G. Cesare-Policlinico, 70125, Bari, Italy.
Zanotti
F
F
Raho
G
G
Papa
S
S
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003958:Diamide / Q000494:pharmacology; D004220:Disulfides / Q000737:chemistry*; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D009840:Oligomycins / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism*; D011522:Protons; D013439:Sulfhydryl Reagents / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
D003432:Cross-Linking Reagents; D004220:Disulfides; D009840:Oligomycins; D011522:Protons; D013439:Sulfhydryl Reagents; D014475:Uncoupling Agents; D003958:Diamide; D006180:Proton-Translocating ATPases