The Arabidopsis thaliana genome encodes at least four thioredoxins m and a new prokaryotic-like thioredoxin.
Screening of cDNA libraries at low stringency and complete sequencing of EST clones with homology to thioredoxins allowed us to characterize five new prokaryotic type Arabidopsis thaliana thioredoxins. All present N-terminal extensions with characteristics of transit peptides. Four are clustered in a phylogenetic tree with the chloroplastic thioredoxin m from red and green algae and higher plants, and their transit peptides have typical characteristics of chloroplastic transit peptides. One is clearly divergent and defines a new prokaryotic thioredoxin type that we have named thioredoxin x. Its transit peptide sequence presents characteristics of both chloroplastic and mitochondrial transit peptides. The five corresponding genes are expressed at different levels, but mostly in green tissues and in in-vitro cultivated cells.
Mestres-Ortega
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Laboratoire de Physiologie et Biologie moléculaire des plantes, Université, UMR CNRS 5545 Avenue de Villeneuve (F), 66860, Perpignan, France.
Meyer
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D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D011387:Prokaryotic Cells / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013879:Thioredoxins / Q000235:genetics*; D014018:Tissue Distribution
D018076:DNA, Complementary; D018744:DNA, Plant; D020033:Protein Isoforms; D018749:RNA, Plant; D013879:Thioredoxins