title: Separation by thin-layer chromatography of the most common androgen-derived C19 steroids formed by mammalian cells.
abstract: Several methods have been developed in the past for the separation and identification of closely related steroid hormones. Although these methods are effective, most of them use HPLC-derived systems and are expensive, laborious, or time-consuming. In the course of our studies of the metabolism of dehydroepiandrosterone and androstenedione in tissues, we have modified a previously published technique in such a way that in one TLC step we can separate most of the androgen C19 steroid derivatives produced by mammalian cells. We have used this modified technique for the past 2 years with considerable success and reproducible results, and we find it to be rapid and relatively inexpensive.
authors.affiliation: Laboratories of Ontogeny/Reproduction Unit-CRBR, Laval University Medical Research Center, CHUQ, Québec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Godin
authors.forename: P R
authors.initials: PR
authors.lastname: Provost
authors.forename: D
authors.initials: D
authors.lastname: Poirier
authors.forename: C H
authors.initials: CH
authors.lastname: Blomquist
authors.forename: Y
authors.initials: Y
authors.lastname: Tremblay
mesh_terms: D000728:Androgens / Q000096:biosynthesis / Q000302:isolation & purification*; D002460:Cell Line; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans
chemical_list: D000728:Androgens