title: An antibody-lectin sandwich assay for quantifying protein glycoforms.
abstract: We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein's glycoforms by simply substituting the antibody and lectin with specific alternatives.
authors.affiliation: School of Biology and Biochemistry, Queen's University, Belfast, UK-Northern Ireland.
authors.forename: F T
authors.initials: FT
authors.lastname: Lundy
authors.forename: G B
authors.initials: GB
authors.lastname: Wisdom
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D002621:Chemistry / Q000379:methods; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D000906:Antibodies; D006023:Glycoproteins; D007074:Immunoglobulin G; D037102:Lectins; D002918:Chymotrypsin