An improved method to distinguish Entamoeba histolytica and Entamoeba dispar.
A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. The amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. The method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.
Gomes
M A
MA
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. magomes@mono.icb.ufmg.br
Pesquero
J B
JB
Furst
C
C
Valle
P R
PR
Pesquero
J L
JL
Silva
E F
EF
D000818:Animals; D004747:Entamoeba / Q000145:classification* / Q000235:genetics; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA