title: The rat pkd2 protein assumes distinct subcellular distributions in different organs.
abstract: Mutations in the PKD2 gene account for approximately 15% of all cases of autosomal-dominant polycystic kidney disease. In the present study the cellular distribution of the Pkd2 protein was investigated by immunohistochemistry in different rat organs. Although the Pkd2 protein showed a widespread expression, a strikingly different distribution of the protein was observed between individual organs. Whereas in renal distal tubules and in striated ducts of salivary glands a basal-to-basolateral distribution of Pkd2 was found, a punctate cytoplasmic location was detected in the adrenal gland, ovary, cornea, and smooth muscle cells of blood vessels. Interestingly, in the adrenal gland and ovary, the rat Pkd2 protein was more heavily N-glycosylated than in the kidney and salivary gland. These results suggest that Pkd2 accomplishes its functions by interacting with proteins located in different cellular compartments. The extrarenal expression pattern of the Pkd2 protein hints at other candidate sites of disease manifestations in patients carrying PKD2 mutations.
authors.affiliation: Medical Research Center, Klinikum Mannheim, University of Heidelberg, 68167 Mannheim, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Obermüller
authors.forename: A R
authors.initials: AR
authors.lastname: Gallagher
authors.forename: Y
authors.initials: Y
authors.lastname: Cai
authors.forename: N
authors.initials: N
authors.lastname: Gassler
authors.forename: N
authors.initials: N
authors.lastname: Gretz
authors.forename: S
authors.initials: S
authors.lastname: Somlo
authors.forename: R
authors.initials: R
authors.lastname: Witzgall
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D007672:Kidney Cortex / Q000166:cytology / Q000378:metabolism; D007679:Kidney Medulla / Q000166:cytology / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D009399:Nephrons / Q000166:cytology / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism; D050396:TRPP Cation Channels
chemical_list: D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D050396:TRPP Cation Channels