Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils.
Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.
Liu
Q
Q
Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. liuqiang@cc.hirosaki-u.ac.jp
Suzuki
K
K
Kudo
S
S
Yamada
M
M
Kowatari
K
K
Umeda
T
T
Nakaji
S
S
Sugawara
K
K
D005503:Food Additives / Q000494:pharmacology; D005989:Glycerides; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
D005503:Food Additives; D005989:Glycerides; D017382:Reactive Oxygen Species; C403602:decaglycerol monooleate; D005990:Glycerol