High density lipoprotein catabolism in primary cultured hepatocytes from daunomycin-induced nephrotic rats.
We investigated into HDL (high density lipoprotein) catabolism with primary cultured hepatocytes to elucidate the causes of increased HDL apolipoproteins in the plasma of daunomycin-induced nephrotic rats (D rats). The phospholipid, triglyceride, cholesterol, cholesteryl ester and apolipoprotein contents in HDL increased in D rats compared with control rats (C rats). The uptake (binding plus internalization) of (125)I-HDL from D rats to two groups of hepatocytes was significantly greater than that of (125)I-HDL from C rats. Uptake of (125)I-HDL from D rats to D rats' hepatocytes was significantly greater than that of (125)I-HDL from C rats to C rats' hepatocytes. The degradation of (125)I-HDL from D rats was greater than that of (125)I-HDL from C rats using two groups of hepatocytes. These results demonstrated that the uptake and degradation of HDL to D rats' hepatocytes were greater than those of HDL to C rats' hepatocytes. The increased HDL apolipoprotein content in the plasma of D rats may not be due to decreased uptake and degradation of HDL in hepatocytes compared with C rats.
Gomikawa
S
S
Faculty of Health Science, Kobe University School of Medicine, Kobe, Japan.
Suzuki
K
K
Takamitu
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003630:Daunorubicin / Q000633:toxicity*; D004195:Disease Models, Animal; D004705:Endocytosis; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009404:Nephrotic Syndrome / Q000097:blood* / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D008075:Lipoproteins, HDL; D003630:Daunorubicin