title: The N-terminally acetylated form of mammalian histone H1(o), but not that of avian histone H5, increases with age.
abstract: We report here on the HPCE separation of two chicken H5 histones, which do not show the heterogeneity (Gln/Arg) at residue 15 first found by Greenaway and Murray [Greenaway and Murray (1971) Nat. New Biol. 229, 233-238]. The two subfractions obtained were identified using reversed-phase HPLC, hydrophilic interaction HPLC, Edman degradation, and MALDI-MS analysis. We found that the two H5 subcomponents differ only by an acetylated (designated H5a) and an unacetylated N-terminus (H5b). In contrast to the N-terminally acetylated form of rat kidney histone H1(o), which increased by about 40% with aging of the animal, the corresponding form of chicken H5 did not: the ratio N-terminally acetylated: unacetylated remained constant (30:70) when histone H5 was extracted from erythrocytes of newly hatched chickens and from adult chickens, respectively. The HPCE technique used in this investigation represents a quick and convenient method for analyzing N-terminally acetylated proteins in the presence of unacetylated forms.
authors.affiliation: Institute of Medical Chemistry, University of Innsbruck, Fritz Preglstrasse 3, Innsbruck, A-6020, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Sarg
authors.forename: W
authors.initials: W
authors.lastname: Helliger
authors.forename: B
authors.initials: B
authors.lastname: Hoertnagl
authors.forename: B
authors.initials: B
authors.lastname: Puschendorf
authors.forename: H
authors.initials: H
authors.lastname: Lindner
mesh_terms: D000107:Acetylation; D000375:Aging / Q000502:physiology*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism; D019075:Electrophoresis, Capillary; D004912:Erythrocytes / Q000737:chemistry; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry / Q000166:cytology; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006657:Histones; D002918:Chymotrypsin