Immunoregulatory roles of interleukin-13 in cattle.
Interleukin-13 (IL-13) is produced predominantly by helper T lymphocytes of the Th2 phenotype and mediates its effects on several immune cells, including B lymphocytes and macrophages, stimulating their proliferation, differentiation, and effector functions. IL-13 activates human B cells but has no detectable activity on murine B lymphocytes, suggesting that the activity of IL-13 varies among species. Our studies show that IL-13 enhances proliferation and differentiation of bovine B cells and upregulates cell surface major histocompatibility complex (MHC) class II expression. We examined mRNA expression of the putative signaling component of the bovine IL-13Ralpha1 homolog in several peripheral blood populations. After stimulation with calcium ionophore and phorbol ester, IL-13Ralpha1 mRNA levels appeared to be downmodulated in T cells, upregulated in macrophages and B cells, and unchanged in neutrophils. Together, these studies begin to provide insight into the relative importance of IL-13 in immunoregulation in cattle.
Trigona
W L
WL
University of Missouri-Columbia, Department of Veterinary Pathobiology, 65211, USA.
Hirano
A
A
Brown
W C
WC
Estes
D M
DM
D000276:Adjuvants, Immunologic / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000502:physiology; D001483:Base Sequence; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D018793:Interleukin-13 / Q000502:physiology*; D053661:Interleukin-13 Receptor alpha1 Subunit; D008969:Molecular Sequence Data; D018123:Receptors, Interleukin / Q000235:genetics; D053660:Receptors, Interleukin-13; D012689:Sequence Homology, Nucleic Acid
D000276:Adjuvants, Immunologic; D000949:Histocompatibility Antigens Class II; C508181:IL13RA1 protein, human; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13