The multigene immunophilin family of Dictyostelium discoideum. Characterization of microsomal and mitochondrial cyclophilin isoforms.
The sequences of two cyclophilin (Cyp) isoforms from Dictyostelium discoideum have been determined. cyp2 is expressed as a 197 amino acid protein, which contains a 22 amino acid-long signal sequence, characteristic of endoplasmic reticulum localization signals, and that is cleaved in the mature protein. Mature Cyp2 has a molecular mass of 18 986 Da. The cyp3 gene encodes a 174 amino acid protein with a predicted molecular mass of 19 016 Da. Its sequence reveals no targeting sequence. From the MS analysis of affinity-purified cyclophilins from different subcellular compartments, we localized the previously described Cyp1 (Barisic K. et al., Dev. Genet. 12 (1991) 50-53) in cytosol, Cyp2 in microsomes and Cyp3 in mitochondria, respectively. The expression of cyp1 mRNA is constant during differentiation, whereas the mRNA level of both cyp2 and cyp3 is regulated and decreases steadily during development.
Tapparo
A
A
Laboratoire de Biochimie et Biophysique des Systèmes Intégrés (UMR 314 CEA-CNRS), Département de Biologie Moléculaire et Structurale, CEA-Grenoble, 17, rue des Martyrs, 38054 Grenoble, cedex 09 France.
Kieffer
S
S
Cretin
F
F
Satre
M
M
Klein
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics*; D017125:Genes, Protozoan; D020104:Immunophilins / Q000235:genetics*; D008861:Microsomes / Q000737:chemistry; D008928:Mitochondria / Q000737:chemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D020033:Protein Isoforms / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D020033:Protein Isoforms; D015800:Protozoan Proteins; D020104:Immunophilins