title: Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice.
abstract: The growth and spread of tumour cells depends on adequate vasculature. We have previously reported that the expression of interleukin-8 (IL-8) directly correlates with the vascularity of human gastric carcinomas. To provide evidence for a causal role of IL-8 in angiogenesis and tumorigenicity of human gastric cancer, we used the lipofectin method to stably transfect the human TMK-1 gastric carcinoma cells (low endogenous IL-8) with an IL-8 expression vector or control vector. Transfection with IL-8 did not affect the proliferation of cultured cells, yet the culture supernatants of the transfected (but not control) cells stimulated proliferation of human umbilical vein endothelial cells. The IL-8-transfected and control cells were injected into the gastric wall of nude mice. IL-8-transfected cells produced rapidly growing, highly vascular neoplasms as compared to control cells. These results provide direct evidence for the role of IL-8 in the angiogenesis and tumorigenicity of human gastric carcinomas.
authors.affiliation: First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kitadai
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Haruma
authors.forename: K
authors.initials: K
authors.lastname: Naka
authors.forename: K
authors.initials: K
authors.lastname: Sumii
authors.forename: H
authors.initials: H
authors.lastname: Yokozaki
authors.forename: W
authors.initials: W
authors.lastname: Yasui
authors.forename: N
authors.initials: N
authors.lastname: Mukaida
authors.forename: Y
authors.initials: Y
authors.lastname: Ohmoto
authors.forename: G
authors.initials: G
authors.lastname: Kajiyama
authors.forename: I J
authors.initials: IJ
authors.lastname: Fidler
authors.forename: E
authors.initials: E
authors.lastname: Tahara
mesh_terms: D000818:Animals; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000209:etiology*; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000209:etiology / Q000473:pathology; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D016209:Interleukin-8