Hypertrophic stimuli augment expression of cMG1/ERF-1, a putative zinc-finger motif transcription factor, in rat cardiomyocytes.
We isolated the gene for cMG1/ERF-1, a known putative zinc-finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF-1 mRNA at 15 min, and levels peaked at 10-fold initial levels at 30 min. cMG1/ERF-1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin-1 also induced cMG1/ERF-1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF-1 expression. To our knowledge, this is the first report showing that the cMG1/ERF-1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.
Manabe
T
T
Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Fukuda
K
K
Pan
J
J
Nagasaki
K
K
Yamaguchi
K
K
Ogawa
S
S
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D051817:Butyrate Response Factor 1; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology; D017874:Immediate-Early Proteins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013314:Stress, Mechanical; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis; D051816:Tristetraprolin; D016335:Zinc Fingers
D051817:Butyrate Response Factor 1; D004268:DNA-Binding Proteins; D019332:Endothelin-1; D004791:Enzyme Inhibitors; D006131:Growth Inhibitors; D017874:Immediate-Early Proteins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051816:Tristetraprolin; C497554:Zfp36 protein, rat; C497555:Zfp36l1 protein, rat; D000804:Angiotensin II; D011505:Protein-Tyrosine Kinases