title: [DNA methyltransferases for detection of the level of methylation of cytosine in the DNA CCWGG sequence].
abstract: An assay for the cytosine methylation level in the eukaryotic DNA CCWGG sequence is proposed. The method is based on the ability of DNA methylase BstNI to methylate DNA containing in a CCWGG site a nonmodified or 5-methylated cytosine to yield N4-methyl- or N4,5'-dimethylcytosine, respectively.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Moscow, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Shevchuk
authors.forename: Ia I
authors.initials: IaI
authors.lastname: Bur'ianov
mesh_terms: D044503:5-Methylcytosine; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D019175:DNA Methylation; D004926:Escherichia coli / Q000378:metabolism
chemical_list: D044503:5-Methylcytosine; D003596:Cytosine; D004248:DNA (Cytosine-5-)-Methyltransferases
vernacular_title: DNK-metiltransferazy dlia opredeleniia urovnia metilirovaniia tsitozina v posledovatel'nosti CCWGG DNK.