title: Enhancement of both reaction yield and rate of synthesis of structured triacylglycerol containing eicosapentaenoic acid under vacuum with water activity control.
abstract: Production of structured triacylglycerols (sTAG) containing eicosapentaenoic acid (EPA) at the sn-1 (or 3) position using Lipozyme in a solvent-free system was studied. Optimal water activity (a(w)) for the synthesis of the sTAG was investigated. Vacuum was applied to shift reaction equilibrium toward the synthesis reaction by removing by-products. During vacuum application, the water level of the reaction system was controlled at the optimal level by addition of a suitable amount of water at a predetermined interval. Intermittent periodic addition of a suitable amount of water into the reaction mixture made the reaction rate faster than that without adding water. A molar yield of 89.7% of the targeted sTAG was obtained after 16 h reaction with a(w) control during the vacuum application as compared with the yield of 87.0% after 24 h of reaction without a(w) control during the vacuum application.
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bio- and Agro-Sciences, Nagoya University, Japan.
authors.forename: J J
authors.initials: JJ
authors.lastname: Han
authors.forename: T
authors.initials: T
authors.lastname: Yamane
mesh_terms: D000327:Adsorption; D002210:Caprylates / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D015118:Eicosapentaenoic Acid / Q000032:analysis*; D004800:Enzymes, Immobilized; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D055585:Physical Phenomena; D010825:Physics; D014280:Triglycerides / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D014618:Vacuum; D014867:Water / Q000737:chemistry*
chemical_list: D002210:Caprylates; D004800:Enzymes, Immobilized; D014280:Triglycerides; D014867:Water; C003637:tricaprylin; D015118:Eicosapentaenoic Acid; C093666:Lipozyme; D008049:Lipase