title: The effects of targeting the vaccinia virus B5R protein to the endoplasmic reticulum on virus morphogenesis and dissemination.
abstract: The consequence of redirecting the vaccinia virus (VV) B5R protein to the endoplasmic reticulum (ER) has been investigated by the addition of an ER retrieval signal KKSL (K(2)X(2)) to the B5R C-terminus. This mutant B5R gene and a version of the gene with the inactive ER retrieval sequence KKSLAL (K(2)X(4)) were inserted into the thymidine kinase locus of a VV mutant lacking the B5R gene, vDeltaB5R. Similar levels of B5R protein were made by each virus, but the B5R-K(2)X(2) protein remained sensitive to endoglycosidase H and colocalised with protein disulphide isomerase in the ER. In contrast, the B5R-K(2)X(4) protein colocalised with 1, 4-galactosyltransferase in the trans-Golgi network. Electron microscopy revealed that even when the B5R protein was redirected to the ER, intracellular mature virus particles were wrapped by cellular membranes to form intracellular enveloped virus particles, although more incompletely wrapped particles were evident compared with wild type. These intracellular enveloped virus particles were, however, unable to efficiently induce the polymerisation of actin and the plaque size formed by vB5R-K(2)X(2) was small. Nevertheless, the amount and specific infectivity of EEV produced by vB5R-K(2)X(2) were similar to those of wild type, despite the dramatic reduction in the amount of B5R protein present in vB5R-K(2)X(2) EEV.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom.
authors.forename: E C
authors.initials: EC
authors.lastname: Mathew
authors.forename: C M
authors.initials: CM
authors.lastname: Sanderson
authors.forename: R
authors.initials: R
authors.lastname: Hollinshead
authors.forename: M
authors.initials: M
authors.lastname: Hollinshead
authors.forename: R
authors.initials: R
authors.lastname: Grimley
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
mesh_terms: D000199:Actins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology*; D008854:Microscopy, Electron; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000254:growth & development* / Q000472:pathogenicity; D014759:Viral Envelope Proteins / Q000502:physiology*
chemical_list: D000199:Actins; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C074512:42kDa protein, Vaccinia virus; D006596:Hexosaminidases; D019704:Protein Disulfide-Isomerases