Identification of discriminator base atomic groups that modulate the alanine aminoacylation reaction.
Specific aminoacylation of tRNAs involves activation of an amino acid with ATP followed by amino acid transfer to the tRNA. Previous work showed that the transfer of alanine from Escherichia coli alanyl-tRNA synthetase to a cognate RNA minihelix involves a transition state sensitive to changes in the tRNA acceptor stem. Specifically, the "discriminator" base at position 73 of minihelix(Ala) is a critical determinant of the transfer step of aminoacylation. This single-stranded nucleotide has previously been shown by solution NMR to be stacked predominantly onto G(1) of the first base pair of the alanine acceptor stem helix. In this work, RNA duplex(Ala) variants were prepared to investigate the role of specific discriminator base atomic groups in aminoacylation catalytic efficiency. Results indicate that the purine structure appears to be important for stabilization of the transition state and that major groove elements are more critical than those located in the minor groove. This result is in accordance with the predicted orientation of a class II synthetase at the end of the acceptor helix. In particular, substitution of the exocyclic amino group of A(73) with a keto-oxygen resulted in negative discrimination at this site. Taken together, these new results are consistent with the involvement of major groove atomic groups of the discriminator base in the formation of the transition state for the amino acid transfer step.
Fischer
A E
AE
Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Beuning
P J
PJ
Musier-Forsyth
K
K
D000215:Acylation; D000409:Alanine / Q000378:metabolism*; D000412:Alanine-tRNA Ligase / Q000378:metabolism*; D012344:RNA, Transfer, Ala / Q000737:chemistry*; D013329:Structure-Activity Relationship
D012344:RNA, Transfer, Ala; D000412:Alanine-tRNA Ligase; D000409:Alanine