Serial analysis of gene expression in HIV-1-infected T cell lines.
The gene expression profile of the HIV-1 infection state was analyzed in the human T cell line MOLT-4. Using the serial analysis of gene expression (SAGE) method, a total of 142¿ omitted¿603 SAGE tags were sequenced and identified, representing 43¿ omitted¿581 unique mRNA species. Comparison of expression patterns revealed that 53 cellular genes were differentially expressed upon HIV-1 infection. Northern blot and RT-PCR analyses confirmed the altered expression of the genes in both MOLT-4 and MT-4 cells. Up-regulated genes were mainly composed of transcription factors and genes related to T cell activation, whereas down-regulated genes were comprised of mitochondrial proteins, actin-related factors and translational factors. These findings indicate that persistent T cell activation, which may accelerate HIV-1 replication, and the disruption of cellular housekeeping genes including those involved in anti-apoptotic systems, may play an important role in HIV-1-induced pathogenesis.
Ryo
A
A
Departments of Microbiology and Molecular Virology, School of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, Japan.
Suzuki
Y
Y
Ichiyama
K
K
Wakatsuki
T
T
Kondoh
N
N
Hada
A
A
Yamamoto
M
M
Yamamoto
N
N
D017209:Apoptosis / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015658:HIV Infections; D015497:HIV-1; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008213:Lymphocyte Activation; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D051034:Inhibitor of Apoptosis Proteins; D011506:Proteins; D012333:RNA, Messenger