title: Secretion of cryparin, a fungal hydrophobin.
abstract: Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are not glycosylated. This glycosylation occurred in the proprotein region, which is cleaved during maturation by a Kex2-like serine protease, leaving a mature form of cryparin that could be isolated from both the cell wall and culture medium. Pulse-chase labeling experiments showed that cryparin was secreted through the cell wall, without being bound, into the culture medium. The secreted protein then binds to the cell walls of C. parasitica, where it remains. Binding of cryparin to the cell wall occurred in submerged culture, presumably because of the lectin-like properties unique to this hydrophobin. Thus, the binding of this hydrophobin to the cell wall is different from that of other hydrophobins which are reported to require a hydrophobic-hydrophilic interface for assembly.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: McCabe
authors.forename: N K
authors.initials: NK
authors.lastname: Van Alfen
mesh_terms: D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008970:Molecular Weight; D013462:Sulfur Radioisotopes; D013997:Time Factors
chemical_list: C074967:CRP protein, Cryphonectria parasitica; D005656:Fungal Proteins; D008565:Membrane Proteins; D013462:Sulfur Radioisotopes; D003545:Cysteine