The vaccinia virus 39-kDa protein forms a stable complex with the p4a/4a major core protein early in morphogenesis.
The vaccinia virus (VV) 39-kDa protein, the product of the A4L gene, is a highly antigenic protein of the viral core. Pulse-chase and immunoprecipitation experiments have shown that the 39-kDa protein interacts with p4a (encoded by the A10L gene), the precursor of the most abundant virion protein. This interaction is maintained with the processed 4a form that arises during virion maturation. The controlled disruption of mature viral particles showed that the 39-kDa and 4a proteins are tightly bound within the virion. Immunoelectron microscopy showed that both proteins first localize within the cytoplasm and later accumulate inside the viral factories, reaching these locations via a mechanism apparently unrelated to cellular membranes. Double labeling experiments showed a colocalization of both proteins in all virus-induced structures.
Risco
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Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Madrid, 28049, Spain.
Rodríguez
J R
JR
Demkowicz
W
W
Heljasvaara
R
R
Carrascosa
J L
JL
Esteban
M
M
Rodríguez
D
D
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D010450:Endopeptidases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011498:Protein Precursors / Q000378:metabolism; D013997:Time Factors; D014616:Vaccinia virus / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion; D014779:Virus Replication
C069852:P4a protein, Vaccinia virus; D011498:Protein Precursors; C088682:VP39 protein, Vaccinia virus; D014758:Viral Core Proteins; D014764:Viral Proteins; D010450:Endopeptidases