title: Precision, accuracy and linearity of radiometer EML 105 whole blood metabolite biosensors.
abstract: The analytical performance of a new, whole blood glucose and lactate electrode system (EML 105 analyser. Radiometer Medical A/S. Copenhagen, Denmark) was evaluated. Between-day coefficients of variation were < or = 1.9% and < or = 3.1% for glucose and lactate, respectively. Recoveries of glucose were 100 +/- 10% using either aqueous or protein-based standards. Recoveries of lactate depended on the matrix, being underestimated in aqueous standards (approximately -10%) and 95-100% in standards containing 40 g/L albumin at lactate concentrations of 15 and 30 mmol/L. However, recoveries were high (up to 180%) at low lactate concentrations in protein-based standards. Carry-over, investigated according to National Clinical Chemistry Laboratory Standards EP10-T2, was negligible (alpha = 0.01). Glucose and lactate biosensors equipped with new membranes were linear up to 60 and 30 mmol/L, respectively. However, linearity fell upon daily use with increasing membrane lifetime. We conclude that the Radiometer metabolite biosensor results are reproducible and do not suffer from specimen-related carry-over. However, lactate recovery depends on the protein content and the lactate concentration.
authors.affiliation: Department of Clinical Chemistry, Sophia Children's Hospital, Rotterdam, The Netherlands. cobbaert@worldonline.nl
authors.forename: C
authors.initials: C
authors.lastname: Cobbaert
authors.forename: C
authors.initials: C
authors.lastname: Morales
authors.forename: M
authors.initials: M
authors.lastname: van Fessem
authors.forename: H
authors.initials: H
authors.lastname: Kemperman
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D001786:Blood Glucose / Q000032:analysis*; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D011874:Radiometry / Q000295:instrumentation; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D019344:Lactic Acid