Activity-dependent neurotrophic factor peptide (ADNF9) protects neurons against oxidative stress-induced death.
Activity-dependent neurotrophic factor (ADNF) and a 14-amino acid fragment of this peptide (sequence VLGGGSALLRSIPA) protect neurons from death associated with an array of toxic conditions, including amyloid beta-peptide, N-methyl-D-aspartate, tetrodotoxin, and the neurotoxic HIV envelope coat protein gp120. We report that an even smaller, nine-amino acid fragment (ADNF9) with the sequence SALLRSIPA potently protects cultured embryonic day 18 rat hippocampal neurons from oxidative injury and neuronal apoptosis induced by FeSO4 and trophic factor withdrawal. Among the characteristics of this protection are maintenance of mitochondrial function and a reduction in accumulation of intracellular reactive oxygen species.
Glazner
G W
GW
Sanders-Brown Research Center on Aging and Development of Anatomy and Neurobiology, University of Kentucky, Lexington 40536-0230, USA.
Boland
A
A
Dresse
A E
AE
Brenneman
D E
DE
Gozes
I
I
Mattson
M P
MP
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D008928:Mitochondria / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species
D016895:Culture Media, Serum-Free; D005296:Ferrous Compounds; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; C020748:ferrous sulfate