Initial assessment of gene diversity for the oomycete pathogen Phytophthora infestans based on expressed sequences.
A total of 1000 expressed sequence tags (ESTs) corresponding to 760 unique sequence sets were identified using random sequencing of clones from a cDNA library constructed from mycelial RNA of Phytophthora infestans. A number of software programs, represented by a relational database and an analysis pipeline, were developed for the automated analysis and storage of the EST sequence data. A set of 419 nonredundant sequences, which correspond to a total of 632 ESTs (63.2%), were identified as showing significant matches to sequences deposited in public databases. A putative cellular identity and role was assigned to all 419 sequences. All major functional categories were represented by at least several ESTs. Four novel cDNAs containing sequences related to elicitins, a family of structurally related proteins that induce the hypersensitive response and condition avirulence of P. infestans on Nicotiana plants, were among the most notable genes identified. Two of these elicitin-like cDNAs were among the most abundant cDNAs examined. The set also contained several ESTs with high sequence similarity to unique plant genes.
Kamoun
S
S
Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691, USA.
Hraber
P
P
Sobral
B
B
Nuss
D
D
Govers
F
F
D000199:Actins / Q000235:genetics / Q000378:metabolism; D020418:Algal Proteins / Q000235:genetics; D000465:Algorithms; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D014644:Genetic Variation; D010802:Phylogeny; D010838:Phytophthora / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010947:Plants, Toxic; D011506:Proteins; D017422:Sequence Analysis, DNA; D012984:Software; D014026:Nicotiana / Q000382:microbiology
D000199:Actins; D020418:Algal Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins; D011506:Proteins; C085101:elicitin, Phytophthora