title: Muscle-specific up-regulation of rat UCP3 mRNA expression by long-term hindlimb unloading.
abstract: The effect of long-term hindlimb unloading (2 or 5 week) on the expression of uncoupling protein-3 (UCP3) gene was investigated in rat skeletal muscles. The interaction of hindlimb unloading and thyroid status was also investigated at 2 weeks. Whatever the duration, mechanical unloading induced a similar increase in UCP3 mRNA relative abundance in the slow-twitch soleus (SOL) muscle (+80%, P < 0.05), whereas no effect was observed in the fast-twitch extensor digitorum longus (EDL) muscle. Hypothyroidism down-regulated while hyperthyroidism up-regulated UCP3 mRNA relative abundance in both SOL and EDL muscles, but thyroid status did not prevent the up-regulation of UCP3 induced by 2 weeks of suspension. These data therefore indicate for the first time that long-term hindlimb unloading up-regulates muscle UCP3 gene expression in a muscle-specific manner which is independent of thyroid status.
authors.affiliation: Cellulaires et Moléculaires, UMR 5578 CNRS-Université Cl. Bernard Lyon I, 43 Boulevard du 11 Novembre 1918, Villeurbanne, F-69622, France.
authors.forename: F
authors.initials: F
authors.lastname: Denjean
authors.forename: D
authors.initials: D
authors.lastname: Desplanches
authors.forename: J
authors.initials: J
authors.lastname: Lachuer
authors.forename: F
authors.initials: F
authors.lastname: Cohen-Adad
authors.forename: M H
authors.initials: MH
authors.lastname: Mayet
authors.forename: C
authors.initials: C
authors.lastname: Duchamp
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D006614:Hindlimb; D006980:Hyperthyroidism / Q000235:genetics; D007037:Hypothyroidism / Q000235:genetics; D007473:Ion Channels; D024101:Mitochondrial Proteins; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000378:metabolism; D000071247:Uncoupling Protein 3
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604394:Ucp3 protein, rat; D000071247:Uncoupling Protein 3