Transcription of some PHO genes in Saccharomyces cerevisiae is regulated by spt7p.
Spt7p is a new global transcription factor in Saccharomyces cerevisiae(Gansheroff et al., 1995). We report here that the activities of high affinity phosphate transport and acid phosphatase in particular were decreased in a spt7 null mutant. Northern blot experiments revealed that transcription of the PHO84 and PHO5 genes was impaired in this mutant; expression of the PHO regulatory genes, PHO4 and PHO2, was normal. Spt7p is thus linked with expression of several structural genes of the PHO regulon in yeast.
Nishimura
K
K
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Yasumura
K
K
Igarashi
K
K
Harashima
S
S
Kakinuma
Y
Y
D000135:Acid Phosphatase / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D010710:Phosphates / Q000378:metabolism; D028081:Proton-Phosphate Symporters; D015347:RNA Probes / Q000737:chemistry; D012331:RNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012588:Scintillation Counting; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D002352:Carrier Proteins; D005656:Fungal Proteins; C105495:PHO84 protein, S cerevisiae; D010710:Phosphates; D028081:Proton-Phosphate Symporters; D015347:RNA Probes; D012331:RNA, Fungal; C093107:SPT7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000135:Acid Phosphatase