Inhibitory effect of Chinese green tea on endothelial cell-induced LDL oxidation.
Green tea has been shown to inhibit Cu(2+)-induced LDL oxidation and suppress lipoxygenase activity. Since LDL oxidation is a characteristic feature of atherogenesis and lipoxygenase is involved in the disease process, the effect of Lung Chen Tea, a non-fermented Chinese green tea, on LDL oxidation induced by human umbilical cord vascular endothelial cell was investigated in the present study. Lung Chen Tea was extracted with methanol and the dried powder was redissolved in water before extraction with chloroform and then ethyl acetate. Lung Chen Tea, chloroform and ethyl acetate fractions dose-dependently reduced LDL oxidation and decreased its relative electrophoretic mobility (P<0.001) when compared to the oxidized LDL. The lipid peroxidation products, thiobarbituric acid reactive substances, and cellular cholesterol were also significantly lowered by 5 and 10 microg/ml Lung Chen Tea (P<0.001) in a dose-dependent manner. The remaining aqueous layer, which was devoid of catechins after chloroform and ethyl acetate extractions, did not prevent LDL oxidation. The results of this study demonstrated that Lung Chen Tea and catechin-rich fractions significantly prevented endothelial cell induced LDL oxidation. The consumption of Lung Chen Tea may therefore lower the risk of coronary heart diseases.
Yang
T T
TT
Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, 1/F Li Shu Fan Building, 5 Sassoon Road, Hong Kong.
Koo
M W
MW
D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004586:Electrophoresis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D013662:Tea / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D003470:Culture Media; D008077:Lipoproteins, LDL; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; C099252:oxidized low density lipoprotein; D002784:Cholesterol