Effects of recombinant bovine granulocyte-macrophage colony-stimulating factor on bovine peripheral blood neutrophil functions in vitro and in vivo.
Effects of recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF) on bactericidal activity of bovine peripheral blood neutrophils in vitro and in vivo were studied. In in vitro experiment, bovine blood neutrophils were cultured for 9 hr in media containing 0.005, 0.05 or 0.5 microg/ml of rboGM-CSF. Neutrophils treated with rboGM-CSF showed significantly higher luminol-dependent chemiluminescence (LDCL) than control cells. In in vivo experiment, neutrophils isolated from cows injected 5.0 microg/kg of rboGM-CSF showed significantly higher Nitrobluetetrazolium (NBT) reduction value than that from control cows 24 hr post injection. Total leukocyte counts of cows injected rboGM-CSF sharply decreased 6 hr post injection and recovered to normal level 2 days post injection. Body temperature of these cows rose 6 hr post injection and back to normal level at 24 hr post injection. It was suggested that rboGM-CSF enhanced bactericidal activity of bovine neutrophils both in vitro and in vivo.
Hirai
T
T
Hokkaido Prefectural Shintoku Animal Husbandry Experiment Station, Japan.
Oikawa
M
M
Inumaru
S
S
Yokomizo
Y
Y
Kusakari
N
N
Mori
K
K
D000818:Animals; D001419:Bacteria / Q000276:immunology; D002417:Cattle / Q000097:blood / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D007202:Indicators and Reagents / Q000737:chemistry; D007958:Leukocyte Count / Q000662:veterinary; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D010587:Phagocytosis / Q000187:drug effects; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology
D007202:Indicators and Reagents; D011994:Recombinant Proteins; D009580:Nitroblue Tetrazolium; D008165:Luminol; D016178:Granulocyte-Macrophage Colony-Stimulating Factor