title: Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS NMR using lanthanide ions.
abstract: A simple but efficient (13)C MAS NMR method is presented for the determination of the location of embedded molecules such as peptides relative to biological membrane surfaces by exploiting the interaction with paramagnetic lanthanide ions. Using various aqueous Dy(3+) concentrations a distance-dependent differential paramagnetic quenching of NMR lipid resonance intensities for specific carbon sites was observed, with residues at the bilayer surface quenched effectively and hydrophobic sites unaffected by Dy(3+). Tested on the membrane-embedded 50 residue long M13 coat protein, (13)C labeled at its Val-29 and Val-31 residues, no paramagnetic quenching was observed for the peptide resonances by Dy(3+), suggesting that Val-29 and Val-31 are not in close proximity to the bilayer interface, but buried deeply inside the hydrophobic region of the lipid bilayer.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom. gerhard@bioch.ox.ac.uk
authors.forename: G
authors.initials: G
authors.lastname: Gröbner
authors.forename: C
authors.initials: C
authors.lastname: Glaubitz
authors.forename: A
authors.initials: A
authors.lastname: Watts
mesh_terms: D002247:Carbon Isotopes; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000737:chemistry*; D008674:Metals, Rare Earth; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008674:Metals, Rare Earth; D010455:Peptides; D004134:Dimyristoylphosphatidylcholine