title: Volume-dependent collection of peripheral blood progenitor cells during large-volume leukapheresis for patients with solid tumours and haematological malignancies.
abstract: We investigated the efficacy of peripheral blood progenitor cell (PBPC) collection during large-volume leukapheresis (LVL) in patients with solid tumours and haematological malignancies (n = 18). The time- and volume-dependent harvest of leucocytes (WBC), mononuclear cells (MNC), CD34+ cells and colony-forming cells (CFU-GM) during LVL was analysed in six sequentially filled collection bags processing four times the patient's blood volumes. The amounts of leucocytes (WBC) and the purity of mononuclear cells (MNC%) did not show any significant changes during LVL. The percentage of CD34+ cells remained constant for the first three bags but consecutively decreased from initially 1.71% CD34+ cells in the beginning of LVL to finally 1.34% CD34+ cells (P = 0.02). The mean numbers of colony-forming cells (CFU-GM) decreased from 74 microL-1 to 59 microL-1 during LVL (P = 0.16). Furthermore, the comparison of volume-dependent PBPC collection for patients with high, medium and low total yields of CD34+ cells showed similar kinetics on different levels for the three groups. We concluded that - relative to the initial total amount of PBPC harvested - comparable numbers of progenitor cells can be collected during all stages of LVL with a slight decreasing trend processing four times the patient's blood volumes.
authors.affiliation: Department of Transfusion Medicine, University Hospital Muenster, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Cassens
authors.forename: P
authors.initials: P
authors.lastname: Ostkamp-Ostermann
authors.forename: N
authors.initials: N
authors.lastname: van der Werf
authors.forename: H
authors.initials: H
authors.lastname: Garritsen
authors.forename: H
authors.initials: H
authors.lastname: Ostermann
authors.forename: W
authors.initials: W
authors.lastname: Sibrowski
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000097:blood; D001810:Blood Volume; D002648:Child; D002675:Child, Preschool; D019337:Hematologic Neoplasms / Q000628:therapy*; D006412:Hematopoietic Stem Cells; D006801:Humans; D007700:Kinetics; D007937:Leukapheresis / Q000379:methods*; D007958:Leukocyte Count; D008875:Middle Aged
chemical_list: D018952:Antigens, CD34