title: Dynamics and mobility of nuclear envelope proteins in interphase and mitotic cells revealed by green fluorescent protein chimeras.
abstract: Understanding how membrane proteins are targeted to and retained within the nuclear envelope (NE) and the fate of these proteins during NE disassembly/reassembly in mitosis is central for insight into the function of the NE in nuclear organization and dynamics. To address these issues we have attached green fluorescent protein (GFP) to a well-characterized protein of the inner nuclear membrane, lamin B receptor, believed to be one of the major chromatin docking protein in the NE. We have used this construct in a variety of applications, including dual-color GFP time-lapse imaging, to investigate the mechanisms underlying protein targeting to the NE and NE breakdown and reassembly during mitosis. In this review, we present a summary of the results from such studies and discuss the photobleaching and imaging methodology on which they were derived.
authors.affiliation: Gene Expression and Cell Biology/Biophysics Programmes, EMBL, Menehofstrasse 1, Heidelberg, D-69117, Germany. Jan.Ellenberg@EMBL-Heidelberg.de
authors.forename: J
authors.initials: J
authors.lastname: Ellenberg
authors.forename: J
authors.initials: J
authors.lastname: Lippincott-Schwartz
mesh_terms: D000818:Animals; D019556:COS Cells; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000493:pharmacokinetics*; D007399:Interphase / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000493:pharmacokinetics*; D008938:Mitosis / Q000502:physiology; D008967:Molecular Biology / Q000379:methods*; D009685:Nuclear Envelope / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000493:pharmacokinetics
chemical_list: D007202:Indicators and Reagents; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins