title: Detection of Borna disease virus RNA from peripheral blood cells in schizophrenic patients and mental health workers.
abstract: Accumulating evidence suggests that Borna disease virus (BDV), a neurotropic, negative-stranded RNA virus, might be associated with certain human mental disorders. Several research groups reported that psychiatric patients had a significantly higher prevalence of BDV serum antibodies than normal controls. In addition, a significantly higher presence of BDV RNA from peripheral blood cells was identified in mental patients than in controls. In our previous study, we first identified the presence of BDV serum antibodies in a cohort of Chinese schizophrenic patients from Taiwan, and we also demonstrated a significantly higher seroprevalence of BDV antibodies among schizophrenic patients than in non-psychiatric controls. Prompted by the positive seroepidemiological result, we set out to investigate the detection of BDV RNA from the peripheral blood cells of our schizophrenic patients. By using the reverse transcription-polymerase chain reaction (RT-PCR) method, 10 out of 74 Chinese schizophrenic patients from Taiwan were found to have BDV RNA in their blood cells, whereas only one out of 69 controls was positive. The BDV RNA detection rate among schizophrenic patients was significantly higher than that in controls (14% vs 1.4%, P < 0.01). Furthermore, we studied the BDV RNA detection rate among mental health workers, and seven out of 45 mental health workers were found to have positive results. The prevalence rate was significantly higher than that in normal controls (15% vs 1.4%, P < 0.001), which lends further support to our previous finding that mental health workers have a significantly higher presence of BDV serum antibodies. In summary, our data support the finding that BDV infection might be a contributory factor to the pathogenesis of schizophrenia in the Chinese population.
authors.affiliation: Department of Psychiatry, Tzu-Chi General Hospital, Hualien City, Taiwan. cchen@mail.tcu.edu.tw
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
authors.forename: Y L
authors.initials: YL
authors.lastname: Chiu
authors.forename: C K
authors.initials: CK
authors.lastname: Shaw
authors.forename: M T
authors.initials: MT
authors.lastname: Tsai
authors.forename: A L
authors.initials: AL
authors.lastname: Hwang
authors.forename: K J
authors.initials: KJ
authors.lastname: Hsiao
mesh_terms: D000328:Adult; D015139:Blotting, Southern; D001890:Borna Disease / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission; D001891:Borna disease virus / Q000235:genetics / Q000302:isolation & purification*; D003428:Cross Infection / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006282:Health Personnel; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012559:Schizophrenia / Q000453:epidemiology / Q000821:virology*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral