title: Release of cytochrome c from mitochondria to cytosol in gerbil hippocampal CA1 neurons after transient forebrain ischemia.
abstract: We examined cytosolic cytochrome c in gerbil hippocampal CA1 and CA3 regions after induction of 5-min ischemia by immunoblotting. In the CA1 region, cytochrome c was detected in the cytosolic fraction from 1 to 6 h after ischemia by Western blotting, while it was not detected in the CA3 region. Following intraventricular administration of cyclosporin A (CsA), detectable cytosolic cytochrome c was dramatically decreased, and about 80% of CA1 neurons survived after ischemia. The present studies demonstrate that cytochrome c is translocated from mitochondria to the cytosol in the early stage of delayed neuronal cell death, and suggest the involvement of the mitochondrial permeability transition.
authors.affiliation: Department of Neurological Surgery, Ehime University School of Medicine, Shigenobu-cho, Onsen-gun, Ehime, 791-0295, Japan. hinakats@m.ehime-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Nakatsuka
authors.forename: S
authors.initials: S
authors.lastname: Ohta
authors.forename: J
authors.initials: J
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Toku
authors.forename: Y
authors.initials: Y
authors.lastname: Kumon
authors.forename: N
authors.initials: N
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Sakanaka
authors.forename: S
authors.initials: S
authors.lastname: Sakaki
mesh_terms: D000818:Animals; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007276:Injections, Intraventricular; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*
chemical_list: D016572:Cyclosporine; D003576:Electron Transport Complex IV