title: Haplotype analysis of the apolipoprotein E and apolipoprotein C1 loci in Portugal and São Tomé e Príncipe (Gulf of Guinea): linkage disequilibrium evidence that APOE*4 is the ancestral APOE allele.
abstract: The joint distributions of phenotypes from the apolipoprotein E gene (APOE) and from a closely linked restriction site polymorphism at the apolipoprotein C1 locus (APOC1) were studied in population samples from Portugal and São Tomé e Príncipe (Gulf of Guinea), a former Portuguese colony that was originally populated by slaves imported from the African mainland. The frequencies of the APOE alleles (*2, *3, and *4) in Portugal and São Tomé fitted the ranges of variation generally observed in European and African populations, respectively. Haplotype analysis showed that in both populations the strength of linkage disequilibrium was highest for the APOE*2 allele and lowest for the APOE*4 allele, suggesting that the origin of the APOE alleles followed a 4-->3-->2 pathway and thus providing independent confirmation of the results from sequence homology studies with nonhuman primates. In accordance with global trends in the distribution of human genetic variation, the European sample from Portugal presented more intense linkage disequilibrium between APOE and APOC1 than the African sample from São Tomé where, despite the short 4-kb distance that separates the 2 loci, the level of association between the APOC1 alleles and APOE*4 was nonsignificant.
authors.affiliation: Instituto de Patologia e Imunologia Molecular, Universidade do Porto, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Seixas
authors.forename: M J
authors.initials: MJ
authors.lastname: Trovoada
authors.forename: J
authors.initials: J
authors.lastname: Rocha
mesh_terms: D053302:Apolipoprotein C-I; D053327:Apolipoprotein E4; D001056:Apolipoproteins C / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D018595:Colonialism; D004641:Emigration and Immigration; D005787:Gene Frequency / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D011174:Portugal / Q000208:ethnology; D016415:Sequence Alignment; D012940:Social Problems; D014767:United States Virgin Islands
chemical_list: D053302:Apolipoprotein C-I; D053327:Apolipoprotein E4; D001056:Apolipoproteins C; D001057:Apolipoproteins E