The temporal requirement for endothelin receptor-B signalling during neural crest development.
Endothelin receptor B (EDNRB) is a G-protein-coupled receptor with seven transmembrane domains which is required for the development of melanocytes and enteric neurons. Mice that are homozygous for a null mutation in the Ednrb gene are almost completely white and die as juveniles from megacolon. To determine when EDNRB signalling is required during embryogenesis, we have exploited the tetracycline-inducible system to generate strains of mice in which the endogenous Ednrb locus is under the control of the tetracycline-dependant transactivators tTa or rtTA. By using this system to express Ednrb at different stages of embryogenesis, we have determined that EDNRB is required during a restricted period of neural crest development between embryonic days 10 and 12.5. Moreover, our results imply that EDNRB is required for the migration of both melanoblasts and enteric neuroblasts.
Shin
M K
MK
Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
Levorse
J M
JM
Ingram
R S
RS
Tilghman
S M
SM
D000818:Animals; D002432:Cecum / Q000166:cytology / Q000196:embryology; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement / Q000502:physiology; D003106:Colon / Q000166:cytology / Q000196:embryology; D004318:Doxycycline / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008544:Melanocytes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009432:Neural Crest / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction; D013752:Tetracycline / Q000494:pharmacology; D015534:Trans-Activators / Q000378:metabolism
D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D015534:Trans-Activators; D013752:Tetracycline; D004318:Doxycycline