title: The effect of increased intakes of polyunsaturated fatty acids and vitamin E on DNA damage in human lymphocytes.
abstract: The effect of increasing dietary intakes of polyunsaturated fatty acids (PUFAs) and vitamin E on indices of oxidative DNA damage was investigated. Twenty-one healthy male, nonsmokers aged 28.9 +/- 1.3 years participated in a free-living, split plot/change over trial in which half the volunteers consumed diets containing 5% PUFA as food energy for 4 wk and, after a 10 wk washout period, consumed a 15% PUFA diet for another 4 wk. The other volunteers followed an identical protocol, except that they consumed the 15% PUFA diet first. The diets were provided to volunteers either with or without an additional 80 mg dalpha-tocopherol acetate/day; otherwise total fat, carbohydrates, protein, and basal vitamin E contents remained unchanged. DNA damage induced by 200 microM H(2)O(2) in lymphocytes from volunteers as well as endogenous DNA damage in the form of oxidized pyrimidines, measured by alkaline single-cell gel electrophoresis (the comet assay), significantly decreased after consumption of the 5% PUFA diet (P<0.001 and P=0.01, respectively), but significantly increased after consumption of the 15% PUFA diet when alpha-tocopherol levels were in the range of 5-7 mg/day (P=0. 008 and P=0.03, respectively). These changes were abolished by an additional 80 mg dalpha-tocopherol/day. This study indicates that increasing dietary levels of PUFA to 15% may adversely affect some indices of DNA stability. However, increasing the dietary intake of vitamin E by 80 mg/day ameliorates the damaging effects of PUFA. -Jenkinson, A. McE., Collins, A. R., Duthie, S. J., Wahle, K. W. J., Duthie, G. G. The effect of increased intakes of polyunsaturated fatty acids and vitamin E on DNA damage in human lymphocytes.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK. aj@rri.sari.ac.uk
authors.forename: A M
authors.initials: AM
authors.lastname: Jenkinson
authors.forename: A R
authors.initials: AR
authors.lastname: Collins
authors.forename: S J
authors.initials: SJ
authors.lastname: Duthie
authors.forename: K W
authors.initials: KW
authors.lastname: Wahle
authors.forename: G G
authors.initials: GG
authors.lastname: Duthie
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage; D004042:Dietary Fats, Unsaturated / Q000009:adverse effects*; D004347:Drug Interactions; D005231:Fatty Acids, Unsaturated / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D014810:Vitamin E