title: [Prophylactic effectiveness of propolis for immunostimulation: a clinical pilot study].
abstract: OBJECTIVE
  The aim of this pilot investigation was to show the evidence of the prophylactic immunostimulating effectiveness caused by propolis. The immune response was determined by the measurement of the cytokine level in vivo and ex vivo (TNF-alpha, IL-6, IL-8).
  
  
  METHODS
  In an open prospective monocentric study, test persons received Propolis XNP.
  
  
  METHODS
  10 healthy test persons aged between 18 and 45 years, with normal weight and of either sex.
  
  
  METHODS
  Probands received over 13 days 500 mg Propolis XNP (2 capsules) for peroral application in the morning.
  
  
  METHODS
  Significant changes of the investigated cytokine secretion capacity during and after the treatment of propolis compared to the situation without medication.
  
  
  RESULTS
  Although the cytokine plasma levels did not significantly change during the study, propolis led to a significant increase of both the spontaneous (TNF-alpha, p < 0. 05; IL-6, p < 0.01; IL-8, p < 0.02; IL-1beta, not detectable) and LPS(lipopolysaccaride)-induced (TNF-alpha, p < 0.001; IL-6, p < 0. 02; IL-8, p < 0.0005; IL-1beta, p < 0.05) cytokine secretion capacity following short-term ex vivo culture of peripheral blood leukocytes. Whereas the IL-6 secretion capacity further increased during the 13-day application, the IL-8 and TNF-alpha secretion reached a plateau after day 4 and the TNF-alpha secretion even decreased, but the level at day 13 was still significantly higher than at day 0.
  
  
  CONCLUSIONS
  As the cytokine secretion capacity but not the cytokine plasma levels increased significantly during therapy, the prophylactic application of propolis led to a time dependent enhanced immune reactivity without undesired side effects.
authors.affiliation: Börner GmbH Berlin. cbraetter@yahoo.de
authors.forename: C
authors.initials: C
authors.lastname: Brätter
authors.forename: M
authors.initials: M
authors.lastname: Tregel
authors.forename: C
authors.initials: C
authors.lastname: Liebenthal
authors.forename: H D
authors.initials: HD
authors.lastname: Volk
mesh_terms: D000276:Adjuvants, Immunologic; D000328:Adult; D016588:Anticarcinogenic Agents; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011429:Propolis / Q000627:therapeutic use*; D011446:Prospective Studies
chemical_list: D000276:Adjuvants, Immunologic; D016588:Anticarcinogenic Agents; D016207:Cytokines; D011429:Propolis
vernacular_title: Prophylaktische Wirkungen von Propolis zur Immunstimulation: Eine klinische Pilotstudie.