Cerebellar granule cell-specific and inducible expression of Cre recombinase in the mouse.
To develop a cell type-specific and temporal regulation system of gene targeting in the cerebellum, we used the NMDA-type glutamate receptor GluRepsilon3 subunit gene and Cre recombinase-progesterone receptor fusion (CrePR) gene in combination. Injection of the CrePR gene placed under the control of the 10 kb 5' region of the GluRepsilon3 gene into C57BL/6 eggs yielded the ECP25 line that strongly expressed the CrePR mRNA selectively in the granule cells of the cerebellum. Using a transgenic mouse carrying a reporter gene for Cre-mediated recombination, we showed that antiprogestins could induce the recombinase activity of CrePR protein in the cerebellar granule cells of the ECP25 line. Thus, the established mouse line will provide a valuable tool to investigate the mechanism of cerebellar function by manipulating molecules in the temporally regulated and granule cell-specific manner.
Tsujita
M
M
Department of Molecular Neurobiology, School of Medicine, University of Tokyo, Tokyo 113-0033, Japan.
Mori
H
H
Watanabe
M
M
Suzuki
M
M
Miyazaki
J
J
Mishina
M
M
D016475:3T3 Cells; D000818:Animals; D019677:Artificial Gene Fusion; D002460:Cell Line; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology*; D004790:Enzyme Induction; D018390:Gene Targeting; D006727:Hormone Antagonists / Q000494:pharmacology; D017403:In Situ Hybridization; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000201:enzymology*; D011372:Progestins / Q000037:antagonists & inhibitors; D017470:Receptors, Glutamate / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics; D014764:Viral Proteins
D006727:Hormone Antagonists; D011372:Progestins; D017470:Receptors, Glutamate; D011980:Receptors, Progesterone; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases