Dehydroepiandrosterone (DHEA) facilitates liver regeneration after partial hepatectomy in rats.
In this study we investigated whether or not liver regeneration is facilitated by dehydroepiandrosterone (DHEA) after partial (70%) hepatectomy in rats. Treatment with DHEA (300 mg/kg body weight) did not cause any significant increase in the expression ratio of proliferating cell nuclear antigen (PCNA) in sham-operated controls; however, in partially hepatectomized rats it caused a significant increase in the ratio in hepatocytes 24 and 36 hr after hepatectomy. In partially hepatectomized rats, DHEA treatment significantly accelerated the restoration of liver 48, 60, and 72 hr after partial hepatectomy. The restoration rate in DHEA-treated hepatectomized rats at 72 hr was 1.3-fold greater than in partially hepatectomized controls. Treatment with androstenedione (300 mg/kg body weight), the first metabolite of DHEA, did not cause any significant increase in the expression of PCNA in either sham-operated controls or partially hepatectomized rats. These results indicate that DHEA itself promotes the liver regenerative process after partial hepatectomy in rats.
Kitamura
T
T
Third Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan.
Tanaka
K
K
Morita
K
K
Saito
S
S
Kiba
T
T
Numata
K
K
Sekihara
H
H
D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004435:Eating / Q000502:physiology; D006498:Hepatectomy; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000502:physiology; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D018809:Proliferating Cell Nuclear Antigen; D000735:Androstenedione; D003687:Dehydroepiandrosterone