Apolipoprotein CIII from guinea pig (Cavia porcellus) is shorter and less homologous than apolipoprotein CIII from other mammals.
Apolipoprotein (apo) CIII plays an important role in metabolism of triglyceride-rich lipoproteins as a regulator of lipolysis and/or lipoprotein-receptor interaction. With the method of RT-PCR, the cDNA of guinea pig apo CIII was cloned and sequenced. The deduced amino acid sequence of 91 amino acids residues consists of a highly conserved signal peptide of 20 residues and a mature protein of 71 residues. Compared to mouse, rat, dog, bovine and human apo CIII, guinea pig apo CIII has a deletion of eight or nine amino acids at its C-terminus and it shows the lowest degree of homology to the presently known apo CIII sequences. Interestingly, the most conserved areas of guinea pig apo CIII are found in two regions, residues 16-33 and residues 50-69. Corresponding regions in human and dog apo CIII were previously predicted to form amphipathic helices, which are assumed to play important roles in the inhibition of lipoprotein lipase (LPL) and binding to lipid. Our present study could be helpful for the future elucidation of the structure-function relationships and evolution of apo CIII.
Yin
Y
Y
Department of Medical Biochemistry and Biophysics, Umea University, Sweden. yiny@gwgate.nhlbi.nih.gov
Olivecrona
G
G
D000595:Amino Acid Sequence; D000818:Animals; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood* / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D017124:Conserved Sequence; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000737:chemistry; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D021382:Protein Sorting Signals; D011994:Recombinant Proteins