title: Echovirus 9 strain barty non-structural protein 2C has NTPase activity.
abstract: Non-structural protein 2C is known to play a fundamental role in the replication of picornaviruses. Sequence analyses revealed that 2C belongs to a rapidly expanding group of proteins containing a consensus sequence for nucleotide binding (NTB). We report that echovirus 9 polypeptide 2C displays NTPase activity in vitro. In our experiments, several P2 genes were expressed in Escherichia coli as fusion proteins linked to glutathione S-transferase (GST) prior to purification close to homogeneity. In contrast to GST-2B, both GST-2C and GST-2BC showed ATPase as well as GTPase activity indicating that the site for NTB binding and splitting is located in 2C.
authors.affiliation: Institut für Virologie der Universität zu Köln, Fürst-Pückler-Str. 56, 50935, Cologne, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Klein
authors.forename: H J
authors.initials: HJ
authors.lastname: Eggers
authors.forename: B
authors.initials: B
authors.lastname: Nelsen-Salz
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism; D004459:Echovirus 9 / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D043583:Nucleoside-Triphosphatase; D020365:RNA Helicases / Q000096:biosynthesis / Q000378:metabolism*; D014162:Transfection / Q000235:genetics; D014764:Viral Proteins
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins; C116598:2C helicase; D017766:Acid Anhydride Hydrolases; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases; D043583:Nucleoside-Triphosphatase; D020365:RNA Helicases