Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle.
Two enzyme-linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of 0.1 ng homidium per mL serum. Homidium levels were measurable in serum of cattle for over 2 months following administration of a single intramuscular (i.m.) dose at 1 mg/kg bodyweight. The level of sensitivity afforded by these assays makes them potentially useful tools in the pharmacokinetic evaluation of homidium and for investigating drug resistance or causes of drug failure. Assay 2 was chosen as being most suitable for further studies.
Murilla
G A
GA
Radioisotope Laboratory, Kenya Trypanosomiasis Research Institute (KETRI), Kikuyu, Kenya.
Eisler
M C
MC
Peregrine
A S
AS
Ndung'u
J M
JM
Holmes
P H
PH
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004996:Ethidium / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D007273:Injections, Intramuscular; D008297:Male; D011786:Quality Control; D012756:Sheep; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D014352:Trypanosomiasis / Q000097:blood / Q000517:prevention & control / Q000662:veterinary*
D014344:Trypanocidal Agents; D004996:Ethidium