Colony-promoting activity in mice kidneys with phenylhydrazine hemolytic anemia.
Aqueous anemic mice kidney extracts (MKE) were assessed colony-promoting activity (CPA) of hematopoietic progenitor cells in serum-free cultures stimulated by interleukin-3 and erythropoietin (Epo). Mice with hemolytic anemia followed by phenylhydorazine (PHZ) injection for 3 days showed a decrease in the hematocrit (25.4%) and an increase in serum Epo by 14-fold of the control on day 3 after the treatment. At 3 days, the total number of hematopoietic progenitor cells in the bone marrow of PHZ mice decreased by 67% of the control, while these cells in the spleen increased to 22-fold of the control on day 3 and 55-fold on day 6. A significant increase in CPA was observed in MKE prepared from PHZ mice kidneys. Additionally, bone marrow suppressive anemia induced by 5-fluorouracil resulted in enhanced CPA the same as for PHZ mice, but in contrast, anemia with suppression of Epo-production due to nephrotoxicity induced by cisplatin caused a decrease in CPA. These results suggest that CPA in MKE correlates with hematopoietic conditions, and may have a definite role in hematopoiesis through the function of the kidney.
Bamba
T
T
Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.
Kashiwakura
I
I
Murakami
M
M
Honda
M
M
Hayase
Y
Y
Takagi
Y
Y
D000743:Anemia, Hemolytic / Q000473:pathology*; D000818:Animals; D004921:Erythropoietin / Q000097:blood; D006400:Hematocrit; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007668:Kidney / Q000166:cytology; D008297:Male; D051379:Mice; D010659:Phenylhydrazines; D013154:Spleen / Q000166:cytology
D010659:Phenylhydrazines; C030299:phenylhydrazine; D004921:Erythropoietin