Towards obtaining ES cells in the marine fish species Sparus aurata; multipassage maintenance, characterization and transfection.
Animal Embryonic-Stem (ES) cells represents a unique tool in animal genetic manipulation. Though putative ES cells from several species have been reported, only those from mice proved successful. In this work, a long-term embryonic cell culture, derived from the commercial fish (Sparus aurata), is reported. These cells have been in vitro characterized for totipotency and transfected with a GFP plasmid.
Béjar
J
J
Departamento de Genética, Facultad de Ciencias, Universidad de Málaga, Spain.
Hong
Y
Y
Alvarez
M C
MC
D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005398:Fisheries / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D010473:Perciformes / Q000196:embryology* / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection; D019076:Transgenes