[Renal cell carcinoma. Immunohistological study to the expression of the inactive form of the pyruvate kinase].
OBJECTIVE
The inactive form of pyruvatekinase could be established in different tumours. Purpose of this study was to demonstrate the presence or absence of the inactive form of pyruvatekinase in renal cell carcinoma, metastases and benign renal tissue by immunohistology.


METHODS
After deparaffinization of formaline-fixed tissue (5 original tumours, 2 metastases and 5 benign renal tissues) cells were stained (APAAP-method) with Clon DF4 (ScheBo Tech).


RESULTS
All malign tissue showed a positive reaction, inside benign tissue we saw a positive reaction of endothelial cells however we saw no reaction with benign renal cells.


CONCLUSIONS
Renal cell carcinoma and metastatic cells show a strong immunohistological reaction against the inactive form of pyruvatekinase, no reaction of benign renal cells. It should be possible to develop a serological tumour marker for renal cell carcinoma.
Wechsel
H W
HW
Urologische Abteilung der Eberhard-Karls-Universität Tübingen.
Petri
E
E
Feil
G
G
Nelde
H
H
Bichler
K
K
D014408:Biomarkers, Tumor / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000473:pathology* / Q000556:secondary; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000473:pathology*; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D009367:Neoplasm Staging; D011770:Pyruvate Kinase / Q000032:analysis*; D012016:Reference Values
D014408:Biomarkers, Tumor; D011770:Pyruvate Kinase
Nierenzellkarzinom. Immunhistologische Untersuchungen zur Expression der inaktiven Form der Pyruvatkinass.