Enhanced expression of neutrophil NADPH oxidase components in intestine of rats after burn injury.
We have evaluated the accumulation of neutrophils in the gut and their infiltration into the intestinal extravascular spaces in rats subjected to a 25% total body surface area scald burn. The accumulation of neutrophils was assessed via measurements of myeloperoxidase (MPO) activity in the intestinal homogenates, and the immunohistochemical localization of neutrophil NADPH oxidase component proteins (p47phox and p67phox) within the intestinal extravascular spaces determined neutrophil tissue infiltration. MPO measurements demonstrated a 12- and 21-fold increase above the control value in the intestinal tissue at day 1 and day 3 post-burn, respectively, suggesting that a substantial total tissue accumulation of neutrophils occurs in the gut after burn injury. The immunohistochemical staining procedures showed both a definitive presence of the neutrophil in the intestinal extravascular spaces and an enhanced immunoreactivity in neutrophils accumulating in intestine after burn injury. There was no evidence of either the presence of neutrophils in the extravascular regions or any significant neutrophil immunoreactivity to NADPH oxidase component proteins in the intestines of sham control rats. These findings indicate that burn injury causes an enhanced migration of circulating neutrophils into the intestinal interstitial spaces and an upregulation of NADPH oxidase activity in the infiltrating neutrophils.
Fazal
N
N
Burn & Shock Trauma Institute, Loyola University Chicago, Maywood, Illinois 60153, USA.
Knaus
U G
UG
Sabeh
F
F
Gamelli
R L
RL
McNulty
J A
JA
Sayeed
M M
MM
D000818:Animals; D002056:Burns / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D002634:Chemotaxis, Leukocyte; D004790:Enzyme Induction; D007082:Ileum / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D007583:Jejunum / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D008297:Male; D019255:NADPH Oxidases / Q000096:biosynthesis* / Q000235:genetics; D009504:Neutrophils / Q000201:enzymology*; D018384:Oxidative Stress; D009195:Peroxidase / Q000032:analysis; D010750:Phosphoproteins / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D010750:Phosphoproteins; C079789:neutrophil cytosol factor 67K; D009195:Peroxidase; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1