title: Characterisation of lymphocyte subpopulations in the skin and circulation of horses with sweet itch (Culicoides hypersensitivity).
abstract: Circulating lymphocyte numbers are elevated in horses with the allergic skin disease sweet itch and skin lesions are typified by an infiltrate of eosinophils and mononuclear cells, the latter of which have not been fully characterised. The aim of the present study was to characterise the lymphocyte subpopulations in the circulation and skin of ponies with sweet itch by flow cytometry and a newly developed modified alkaline phosphatase immunohistochemical technique. Sweet itch ponies were found to have significantly greater numbers of circulating CD5+ and CD4+ T-lymphocytes than normal animals. Increased numbers of CD3+ T-lymphocytes, most of which were CD4+, and eosinophils were present in the skin of these animals following intradermal injection of a Culicoides antigen extract (97 +/- 21 vs. 449 +/- 49 CD3+ T-lymphocytes/mm2 in deep dermis of vehicle vs. antigen injected sites; 83 +/- 8% CD4+ T-lymphocytes at antigen injected site). T-lymphocytes, which are thought to be important in the pathogenesis of human allergic skin disease, may therefore contribute to the development of sweet itch lesions via the release of cytokines which can cause eosinophil accumulation and activation. An understanding of the pathology of this disease may lead to a more rational approach to therapy.
authors.affiliation: The Royal Veterinary College, Department of Veterinary Basic Sciences, North Mymms, Herts, UK.
authors.forename: J
authors.initials: J
authors.lastname: McKelvie
authors.forename: A P
authors.initials: AP
authors.lastname: Foster
authors.forename: F M
authors.initials: FM
authors.lastname: Cunningham
authors.forename: A S
authors.initials: AS
authors.lastname: Hamblin
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D018956:CD5 Antigens / Q000032:analysis; D002469:Cell Separation; D002519:Ceratopogonidae; D002908:Chronic Disease; D005434:Flow Cytometry; D006734:Horse Diseases / Q000209:etiology / Q000276:immunology*; D006736:Horses; D007124:Immunoenzyme Techniques; D007299:Insect Bites and Stings / Q000150:complications / Q000276:immunology*; D018655:Lymphocyte Count; D011537:Pruritus / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D012621:Seasons; D012882:Skin Tests; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D018956:CD5 Antigens; D000469:Alkaline Phosphatase