CD4(+) T cells induced by virus-like particles expressing a major T cell epitope down-regulate IL-5 production in an ongoing immune response to Der p 1 independently of IFN-gamma production.
It has been previously demonstrated that hybrid Ty virus-like particles (VLP) prime effective CD8(+) and CD4(+) T cell responses. In this study, we investigated the effect of treating mice with Ty VLP carrying the immunodominant epitope of Der p 1 after sensitizing them to the group 1 allergen of the house dust mite Dermatophagoides pteronyssinus (Der p 1), under conditions that induce T(h)2 immunity. We show that i.p. treatment with the hybrid VLP abrogated allergen-specific IL-5 production and reduced allergen-specific cell proliferation. This suppression of the response was mediated by CD4(+) T cells and was not accompanied by an increase in IFN-gamma production.
Hirschberg
S
S
Department of Biology, Imperial College of Science, London SW7 2AZ, UK.
Layton
G T
GT
Harris
S J
SJ
Savage
N
N
Dallman
M J
MJ
Lamb
J R
JR
D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D002478:Cells, Cultured; D015536:Down-Regulation; D018984:Epitopes, T-Lymphocyte; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008925:Mites / Q000276:immunology; D010446:Peptide Fragments / Q000494:pharmacology
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018984:Epitopes, T-Lymphocyte; D006023:Glycoproteins; D016056:Immunodominant Epitopes; D015848:Interleukin-5; D010446:Peptide Fragments; D007371:Interferon-gamma