title: Comparison of antigen binding capabilities of various membrane filters and filter papers in dot immunoassay.
abstract: Antigen binding activities of 25 kinds of filter papers, including nitrocellulose (NC), nylon or polyvinylidine difluoride (PVDF), in the binding of 5 viruses, 3 bacteria, 2 mycoplasmas and 1 chicken serum protein antigens in dot immunoassay were compared. Immobilon affinity membrane type D (IAM-D) was found best in binding viral antigens, followed by Ultrabind SV-450 (SV-450). SV-450 was found best in binding bacterial antigen, followed by Ultrabind US-450 (US-450), IAM-D and NC 0.45 micron. IAM-D was the best in binding mycoplasma antigen, followed by Ultrabind HP, US-450, NC 0.2 micron. Overall, IAM-D had the best capability in the binding of the antigens.
authors.affiliation: Department of Veterinary Medicine, National Pingtung Polytechnic Institute, Neipu, Taiwan, Republic of China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Lin
authors.forename: M C
authors.initials: MC
authors.lastname: Tung
authors.forename: H T
authors.initials: HT
authors.lastname: Sung
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000276:immunology*; D002645:Chickens; D005374:Filtration; D007118:Immunoassay
chemical_list: D000906:Antibodies; D000941:Antigens