In vitro immune function after vaccination with an inactivated, gp120-depleted HIV-1 antigen with immunostimulatory oligodeoxynucleotides.
We examined the effect of a synthetic oligodeoxynucleotide containing unmethylated CpG immunostimulatory sequences (ISS) as an adjuvant for an HIV-1 immunogen (inactivated, gp120-depleted HIV-1 virus particles). The addition of the ISS to HIV-1 in incomplete Freund's adjuvant (IFA) was the optimal combination for the production of HIV-1-specific immune responses as measured by IFN-gamma (p=0.002) and IgG antibody production. Furthermore, the group that was immunized with HIV/IFA/ISS, as well as the group that received HIV-1 in complete Freund's adjuvant (CFA), stimulated significantly more RANTES (a beta-chemokine) (p=0.002) production from lymph node cells. These results suggest that the addition of CpG immunostimulatory sequences to HIV antigens in IFA may optimize HIV-1-specific immune responses and provides further rationale for the testing of ISS in combination with gp120-depleted whole-killed HIV-1 in IFA as a potential preventive or therapeutic HIV-1 vaccine.
Moss
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The Immune Response Corporation, 5935 Darwin Court, Carlsbad, CA 92008, USA. shotdoc@imnr.com
Diveley
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J
Jensen
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Carlo
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D016915:AIDS Vaccines / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D018946:Chemokine CCL5 / Q000096:biosynthesis; D015226:Dinucleoside Phosphates / Q000494:pharmacology*; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000276:immunology*; D016655:HIV Core Protein p24 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008198:Lymph Nodes / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000276:immunology
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D018946:Chemokine CCL5; D015226:Dinucleoside Phosphates; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007371:Interferon-gamma; D009838:Oligodeoxyribonucleotides; D015164:Vaccines, Inactivated; C015772:CpG dinucleotide