title: Does analysis of the antigenic specificities of anti-neutrophil cytoplasmic antibodies contribute to their clinical significance in the inflammatory bowel diseases?
abstract: BACKGROUND
  The clinical relevance of anti-neutrophil cytoplasmic antibodies (ANCA) in inflammatory bowel diseases is unclear. Definition of their antigenic specificities may improve their diagnostic significance.
  
  
  METHODS
  We studied the target antigens of ANCA in 96 patients with ulcerative colitis (UC) and 112 patients with Crohn disease (CD) by indirect immunofluorescence, antigen-specific enzyme-linked immunosorbent assays, and immunodetection on Western blot. We related the presence of antibodies of defined specificity to clinical symptoms.
  
  
  RESULTS
  By indirect immunofluorescence, ANCA were present in 58% of UC patients and in 21% of CD patients. Major antigens were catalase, alpha-enolase, and lactoferrin. In UC, ANCA titers correlated with disease activity. In CD, both ANCA, by indirect immunofluorescence, and antibodies to lactoferrin were associated with colonic localization of the disease. Neither ANCA, by indirect immunofluorescence, nor antibodies of defined specificity were associated with duration of disease, use of medication, or a history of bowel resection.
  
  
  CONCLUSIONS
  ANCA are useful as markers for UC and colonic localization in CD. Definition of the antigenic specificities of ANCA in inflammatory bowel disease does not significantly contribute to their clinical significance.
authors.affiliation: Dept. of Clinical Immunology, University Hospital, Groningen, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Roozendaal
authors.forename: K
authors.initials: K
authors.lastname: Pogány
authors.forename: G
authors.initials: G
authors.lastname: Horst
authors.forename: T G
authors.initials: TG
authors.lastname: Jagt
authors.forename: J H
authors.initials: JH
authors.lastname: Kleibeuker
authors.forename: G F
authors.initials: GF
authors.lastname: Nelis
authors.forename: P C
authors.initials: PC
authors.lastname: Limburg
authors.forename: C G
authors.initials: CG
authors.lastname: Kallenberg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000032:analysis / Q000276:immunology*; D001324:Autoantigens / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001324:Autoantigens