AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis.
Dlk, also known as ZIP kinase, is a serine/threonine kinase that is tightly associated with nuclear structures. Under certain conditions, which require cytoplasmic localization, Dlk can induce apoptosis. In search for interaction partners that might serve as regulators or targets of this kinase we identified apoptosis antagonizing transcription factor (AATF), a nuclear phosphoprotein of 523 amino acids. The 1.8 kb mRNA seems to be ubiquitously expressed. AATF contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. Indeed, a Gal4-BD-AATF fusion protein exhibited strong transactivation activity. Interestingly, AATF interfered with Dlk-induced apoptosis.
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Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117, Bonn, Germany.
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Kögel
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Scheidtmann
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D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000032:analysis; D064550:Death-Associated Protein Kinases; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
C402637:AATF protein, human; C523104:Aatf protein, rat; D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D064550:Death-Associated Protein Kinases; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020930:MAP Kinase Kinase Kinases; C090757:mitogen-activated protein kinase kinase kinase 12