Use of herpesvirus saimiri-immortalized macaque CD4(+) T cell clones as stimulators and targets for assessment of CTL responses in macaque/AIDS models.
Herpesvirus saimiri (HVS), a nonhuman primate gamma herpes virus, was used to immortalize pig-tailed macaque CD4(+) T lymphocytes. The HVS-immortalized T cell lines were used to develop CD4(+) T cell clones from two animals. Three CD4(+) T cell clones were further characterized for the expression of cell surface markers. All expressed CD2, CD4, CD58, CD69 and CD80 and therefore resembled activated T cells. These clones required exogenous IL-2 for efficient growth and were found to be highly susceptible to infection by the challenge virus, Chimeric simian/human immunodeficiency virus (SHIV(KU-1)). They could also be productively infected not only by the quasispecies of the challenge virus (SHIV(KU-1/PDJ) and SHIV(KU-1/PNA), isolated from macaque PDj and PNa, respectively) but also by a different chimeric simian/human immunodeficiency virus (SHIV(89.6P)) and simian immunodeficiency virus (SIV(MAC239)). The virus-infected CD4(+) T cell clones were also used as stimulators for generation of CTL effectors. These effectors exhibited excellent virus-specific lysis in chromium-release assays when syngenic SHIV(KU-1) infected autologous CD4(+) T cell clones were used as targets. The target cell lysis was virus specific, as uninfected control cells showed no or minimal lysis.
Kumar
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Marion Merrell Dow Laboratory of Viral Pathogenesis and Department of Microbiology, Molecular Genetics and Immunology, University Kansas Medical Center, Kansas City, KS 66160, USA. akumar@kumc.edu
Stipp
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Sheffer
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Narayan
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D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000956:Antigens, Viral; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D002678:Chimera / Q000276:immunology; D002999:Clone Cells; D003601:Cytotoxicity Tests, Immunologic; D004195:Disease Models, Animal; D006678:HIV / Q000276:immunology / Q000472:pathogenicity; D015488:HIV Antigens; D006567:Herpesvirus 2, Saimiriine / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008254:Macaca nemestrina; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000472:pathogenicity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015703:Antigens, CD; D000956:Antigens, Viral; D015488:HIV Antigens; D007376:Interleukin-2