Immunolocalisation of NHE3-like immunoreactivity in the gills of the rainbow trout (Oncorhynchus mykiss) and the blue-throated wrasse (Pseudolabrus tetrious).
Na+/H+ exchange has been implicated in models of ion transport across the branchial epithelium of marine and freshwater fishes. In this preliminary study, we present immunohistochemical data using a polyclonal antibody raised against NHE3 which show NHE3-like immunoreactivity (IR) in the gills from a freshwater and a marine teleost species. In both species, branchial epithelial cells demonstrating NHE3-like IR were localised predominantly to the junction between the filament and the secondary lamellae. However, there was a marked difference in the morphology of the NHE3-like immunoreactive epithelial cells between the species. This morphological difference between the species suggests functional differences in the exchanger, which may be related to marine versus freshwater environments.
Edwards
S L
SL
School of Biological & Chemical Sciences, Deakin University, Geelong, Victoria, Australia.
Tse
C M
CM
Toop
T
T
D000818:Animals; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000737:chemistry*; D015151:Immunoblotting; D007150:Immunohistochemistry; D017686:Oncorhynchus mykiss; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis*
D017923:Sodium-Hydrogen Exchangers