title: Assay method for the perfluorooctyl bromide (perflubron) in rat blood by gas chromatography-mass spectrometry.
abstract: This paper describes a GC-MS method (SIM mode) for the analysis of perfluorooctyl bromide (perflubron, I) in rat blood. The chromatographic separation was performed by injection in the split mode using a CP-select 624 CB capillary column. Following destruction of the emulsion by addition of ethanol, the analytical procedure involves a liquid-liquid extraction with 1,1,2-trichlorotrifluoroethane. The bis(F-butyl)ethene (II) was used as internal standard. Observed retention times were 3.22 min for I and 2.32 min for II. Two calibration curves were used; linear detection responses were obtained for concentrations ranging from 0.009 to 0.9 mg/ml and from 0.9 to 13.5 mg/ml. The extraction efficiency averaged 50% for I and 93% for II. Precision ranged from 0.7 to 14%, and accuracy was between 91 and 109%. The limit of quantification was 9 microg/ml. The method validation results indicate that the performance characteristics of the method fulfilled the requirements for assay method for use in pharmacokinetic studies.
authors.affiliation: Département de Biophysique, Faculté de Pharmacie, Université Montpellier 1, France.
authors.forename: M
authors.initials: M
authors.lastname: Audran
authors.forename: M P
authors.initials: MP
authors.lastname: Krafft
authors.forename: J
authors.initials: J
authors.lastname: De Ceaurriz
authors.forename: J C
authors.initials: JC
authors.lastname: Mathurin
authors.forename: M T
authors.initials: MT
authors.lastname: Sicart
authors.forename: B
authors.initials: B
authors.lastname: Marion
authors.forename: F
authors.initials: F
authors.lastname: Fabre
authors.forename: F
authors.initials: F
authors.lastname: Bressolle
mesh_terms: D000818:Animals; D005466:Fluorocarbons / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006207:Half-Life; D006842:Hydrocarbons, Brominated; D007700:Kinetics; D008297:Male; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
chemical_list: D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C003072:perflubron