Evidence that expression of the Vibrio vulnificus hemolysin gene is dependent on cyclic AMP and cyclic AMP receptor protein.
Glucose repressed hemolysin production in Vibrio vulnificus. Promoter activity of the hemolysin gene, vvh, assessed with a vvh-luxCDABE transcriptional fusion, required cyclic AMP (cAMP) and cAMP receptor protein (CRP) in Escherichia coli. Hemolysin production in V. vulnificus increased after the addition of cAMP and was undetectable in a putative crp mutant, suggesting that vvh is also regulated by cAMP-CRP in V. vulnificus.
Bang
Y B
YB
Department of Food Science and Technology, Institute of Biotechnology, Chonnam National University, Kwang-Ju, 500-757.
Lee
S E
SE
Rhee
J H
JH
Choi
S H
SH
D000818:Animals; D000242:Cyclic AMP / Q000502:physiology*; D002373:Cyclic AMP Receptor Protein / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic; D012756:Sheep; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism
D002373:Cyclic AMP Receptor Protein; D006460:Hemolysin Proteins; D000242:Cyclic AMP; D005947:Glucose