title: Induction of a specific antibody response to Bordetella pertussis antigens in cultures of human peripheral blood mononuclear cells.
abstract: The role of specific antibodies in protective immunity to Bordetella pertussis has not yet been clearly defined. In the present work, the induction of a specific antibody response to B. pertussis in cultures of human peripheral blood mononuclear cells (PBMC) was investigated, on the assumption that the capacity of circulating lymphocytes to mount a specific response in vitro may provide a useful parameter for the evaluation of protective immunity. When PBMC from normal adult donors were cultured with a heat-inactivated B. pertussis whole-cell suspension, cells secreting antibodies to pertussis toxin, pertactin and filamentous haemagglutinin were generated consistently. The antibody response peaked between days 7 and 11 of culture and the antibodies produced were exclusively of the IgM class.
authors.forename: E
authors.initials: E
authors.lastname: Giacomini
authors.affiliation: Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Urbani
authors.affiliation: Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: C M
authors.initials: CM
authors.lastname: Ausiello
authors.forename: A L
authors.initials: AL
authors.lastname: Luzzati
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001782:Blood Donors; D001886:Bordetella pertussis / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D007376:Interleukin-2 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000382:microbiology; D008875:Middle Aged; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D007376:Interleukin-2