Localization of huntingtin-interacting protein-2 (Hip-2) mRNA in the developing mouse brain.
Huntingtin-interacting protein-2 (Hip-2) was identified as a human protein specifically associated with huntingtin in vitro, a gene product affected in patients with Huntington disease (HD). It is a ubiquitin-conjugating enzyme identical to the previously characterized bovine E2-25k. We identified the mouse Hip-2 homologue (mHip-2) and examined its distribution patterns in the developing mouse brain in order to gain an insight into the functional significance of the Hip-2 protein in the normal brain as well as in the pathogenesis of HD. As reported with huntingtin, the mHip-2 mRNA expression developed in parallel with neuronal maturation and became distributed widely in the postnatal mouse brain. This spatiotemporal pattern of mHip-2 mRNA expression resembled that of huntingtin. We further demonstrated that mHip-2 mRNA was colocalized with huntingtin-like immunoreactivity in a single neuron. These findings suggested that the Hip-2 interacted with huntingtin in vivo and played an important role in HD pathogenesis.
Tanno
Y
Y
Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima City, Japan. tanno@cc.fmu.ac.jp
Mori
T
T
Yokoya
S
S
Kanazawa
K
K
Honma
Y
Y
Nikaido
T
T
Takeda
J
J
Tojo
M
M
Yamamoto
T
T
Wanaka
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis; D000071058:Huntingtin Protein; D017403:In Situ Hybridization; D008025:Ligases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes
D018076:DNA, Complementary; C506812:Htt protein, mouse; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C100879:UBE2K protein, human; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases