Gas chromatographic-mass spectrometric analysis of veterinary tranquillizers in urine: evaluation of method performance.
A method for analysis of veterinary tranquillizers in urine using gas chromatography-mass spectrometry (GC-MS) is described. Detection limits are 5 microg/l for ketamine, azaperone and the phenothiazines (chlor-, aceto- and propionylpromazine), 10 microg/l for haloperidol, 20 microg/l for xylazine and 50 microg/l for azaperol, recoveries for all analytes were higher than 70%. Method performance in terms of within-batch, between-days and between-analysts reproducibility was studied and found to be acceptable. Compliance with European Union criteria for confirmation of GC-MS "positive" results is evaluated and discussed.
Olmos-Carmona
M L
ML
Laboratorio de Salud Pública, Consejería de Sanidad y Servicios Sociales, Comunidad Autónoma de Madrid, Spain.
Hernández-Carrasquilla
M
M
D000818:Animals; D001376:Azaperone / Q000652:urine; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006220:Haloperidol / Q000652:urine; D007649:Ketamine / Q000652:urine; D010640:Phenothiazines / Q000652:urine; D010879:Piperazines / Q000652:urine; D011725:Pyridines / Q000652:urine; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep / Q000652:urine; D013552:Swine / Q000652:urine; D014149:Tranquilizing Agents / Q000652:urine*; D014730:Veterinary Medicine; D014991:Xylazine / Q000652:urine
D010640:Phenothiazines; D010879:Piperazines; D011725:Pyridines; D014149:Tranquilizing Agents; D001376:Azaperone; C084681:azaperol; D014991:Xylazine; D007649:Ketamine; D006220:Haloperidol