title: Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis.
abstract: The design, fabrication, and operation of a radial capillary array electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10-cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detection channels. Loading of 96 samples in parallel is achieved using a pressurized capillary array system. High-quality separations of 96 pBR322 restriction digest samples are achieved in < 120 s with the microplate system. The practical utility and multicolor detection capability is demonstrated by analyzing 96 methylenetetrahydrofolate reductase (MTHFR) alleles in parallel using a noncovalent 2-color staining method. This work establishes the feasibility of performing high-throughput genotyping separations with capillary array electrophoresis microplates.
authors.affiliation: Chemistry Department, University of California, Berkeley 94720, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: P C
authors.initials: PC
authors.lastname: Simpson
authors.forename: J R
authors.initials: JR
authors.lastname: Scherer
authors.forename: D
authors.initials: D
authors.lastname: Wexler
authors.forename: C
authors.initials: C
authors.lastname: Skibola
authors.forename: M T
authors.initials: MT
authors.lastname: Smith
authors.forename: R A
authors.initials: RA
authors.lastname: Mathies
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*
chemical_list: D009696:Nucleic Acids