Luminal acidification of diverse organelles by V-ATPase in animal cells.
Eukaryotic cells contain organelles bounded by a single membrane in the cytoplasm. These organelles have differentiated to carry out various functions in the pathways of endocytosis and exocytosis. Their lumina are acidic, with pH ranging from 4.5 to 6.5. This article describes recent studies on these animal cell organelles focusing on (1) the primary proton pump (vacuolar-type H(+)-ATPase) and (2) the functions of the organelle luminal acidity. We also discuss similarities and differences between vacuolar-type H(+)-ATPase and F-type ATPase. Our own studies and interests are emphasized.
Futai
M
M
Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan.
Oka
T
T
Sun-Wada
G
G
Moriyama
Y
Y
Kanazawa
H
H
Wada
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D005057:Eukaryotic Cells / Q000201:enzymology / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D015388:Organelles / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases
D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases