title: Prothoracicotropic activity of SBRPs, the insulin-like peptides of the saturniid silkworm Samia cynthia ricini.
abstract: Synthesis and secretion of the insect molting hormone ecdysteroid in the prothoracic glands (PGs) are stimulated by the prothoracicotropic hormone (PTTH) secreted by the brain. Bombyxins, insulin-like peptides of the silkworm Bombyx mori, show prothoracicotropic activity when administered to the saturniid silkworm Samia cynthia ricini, but they are inactive to B. mori itself. Recently, the genes for the bombyxin homologs of S. cynthia ricini (referred to as Samia bombyxin-related peptides, SBRPs) were cloned. To examine the prothoracicotropic activity of SBRPs on S. cynthia ricini, we synthesized two representative molecules, SBRP-A1 and -B1. They promoted pupa-to-adult development with ED(50) of 50 and 10 ng/pupa (EC(50) of 5 and 1 nM), respectively.
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nagata
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: K
authors.initials: K
authors.lastname: Kojima
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: A
authors.initials: A
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Ishizaki
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000196:embryology; D002942:Circular Dichroism; D007301:Insect Hormones / Q000737:chemistry* / Q000494:pharmacology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000494:pharmacology; D011679:Pupa / Q000187:drug effects / Q000254:growth & development; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; D011993:Recombinant Fusion Proteins; C075028:bombyxins; C009089:prothoracicotropic hormone