In vitro inducible nitric oxide synthesis inhibitory active constituents from Fraxinus rhynchophylla.
Bioassay-guided fractionation of an H2O extract of the barks of Fraxinus rhynchophylla has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, ferulaldehyde (1) and scopoletin (3) together with a coumarin, fraxidin (2). Compounds 1 and 3 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner by murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was reflected in the decreased amount of iNOS protein, as determined by Western blotting.
Kim
N Y
NY
Pae
H O
HO
Ko
Y S
YS
Yoo
J C
JC
Choi
B M
BM
Jun
C D
CD
Chung
H T
HT
Inagaki
M
M
Higuchi
R
R
Kim
Y C
YC
D000171:Acrolein / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D010946:Plants, Medicinal / Q000737:chemistry*; D012603:Scopoletin / Q000302:isolation & purification / Q000494:pharmacology*
D003374:Coumarins; D004791:Enzyme Inhibitors; C401933:fraxiden; C075384:coniferaldehyde; D009569:Nitric Oxide; D000171:Acrolein; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D012603:Scopoletin