title: Identification of factor C protein from Streptomyces griseus by microelectrospray mass spectrometry.
abstract: Factor C, an extracellular signal protein of cellular differentiation, was studied and significant homology was found to several zinc finger-type regulatory proteins. The complete amino acid sequence, deduced from the gene, that encodes the protein, did not support the hypothesis that this protein might be a zinc finger-type regulatory protein. However, a theoretical single nucleotide insertion in the gene can result in another similarly sized protein containing about 20 His residues, which would be responsible for the high zinc affinity of factor C. The protein sample was reduced, alkylated and then in-gel digested with trypsin. The peptide fragments were then separated by capillary chromatography and identified by microelectrospray mass spectrometry. Peaks of higher intensity were sequenced by tandem mass spectrometry. The identified peptide fragments and the measured molecular mass of factor C protein also confirmed the original sequence of protein, as there was no shift in the open reading frame.
authors.affiliation: Department of Medical Chemistry, Albert Szent-Györgyi Medical University, Dóm tér 8, H-6720 Szeged, Hungary. szabop@ovmass.mdche.u-szeged.hu
authors.forename: P T
authors.initials: PT
authors.lastname: Szabó
authors.forename: Z
authors.initials: Z
authors.lastname: Kele
authors.forename: Z
authors.initials: Z
authors.lastname: Birkó
authors.forename: F
authors.initials: F
authors.lastname: Szeszák
authors.forename: S
authors.initials: S
authors.lastname: Bíró
authors.forename: T
authors.initials: T
authors.lastname: Janáky
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D013305:Streptomyces griseus / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D010446:Peptide Fragments; C402566:factor C, Streptomyces