Photosensitive liposomes as 'cages' for laser-triggered solute delivery: the effect of bilayer cholesterol on kinetics of solute release.
Liposomes containing acyl chains incorporating azobenzene chromophores have been investigated as potential 'caging' agents for fast solute release. On photolysis, trapped marker dye can be released from gel-phase liposomes within milliseconds. Solute release is markedly sensitive to the presence of cholesterol in the bilayer. Phospholipids bearing one saturated acyl chain and an azobenzene-substituted chain are ineffective as sensitisers unless cholesterol is present, while doubly substituted phospholipids sensitise release in its absence. Cholesterol markedly affects the temperature profile of solute release depending on the host phospholipid chain length. Solute release is not seen for lipid hosts with unsaturated acyl chains.
Bisby
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Biosciences Division, School of Environmental and Life Sciences, University of Salford, Peel Building, Salford, UK.
Mead
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Morgan
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D001391:Azo Compounds / Q000493:pharmacokinetics; D002784:Cholesterol / Q000378:metabolism*; D005452:Fluoresceins / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism* / Q000528:radiation effects; D008081:Liposomes / Q000378:metabolism*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000493:pharmacokinetics; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D001391:Azo Compounds; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D017319:Photosensitizing Agents; D002784:Cholesterol; C009850:azobenzene; C007740:fluorexon