DNA probe analysis for the carriage of enterotoxigenic Clostridium perfringens in feces of a Mexican subpopulation.
Clostridium perfringens has been implicated as a causative agent of foodborne poisoning, infectious diarrhea (not associated with foods), gas gangrene, and several veterinary diseases. Fecal carriage of enterotoxigenic strains of this bacterium appears to be important in the development of infectious diarrhea and as a source of C. perfringens contamination of foods. In this work, carriage of this bacterium in feces of a Mexican population was analyzed. C. perfringens was found in 126 of the 200 fecal samples obtained from healthy individuals from northern Mexico. The samples had an average of 7.4 x 10(3) spores per gram, with the elderly population showing the highest levels. Dot blot analyses using a dig-labeled probe specific for the enterotoxin gene showed that 7% of the samples had isolates with toxigenic potential.
Vela
M
M
Departamento de Microbiología e Inmunología, Universidad A. de Nuevo León, San Nicolás, México.
Heredia
N L
NL
Feng
P
P
Santos García-Alvarado
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D002353:Carrier State / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D003016:Clostridium perfringens / Q000302:isolation & purification*; D015342:DNA Probes; D004768:Enterotoxins / Q000235:genetics*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged
D015342:DNA Probes; D004768:Enterotoxins; C035598:enterotoxin, Clostridium