Noninvasive measurement of brain temperature after stroke.
OBJECTIVE
Brain temperature may be an important factor governing the extent of neuronal injury associated with stroke. The goal of this study was to develop a noninvasive method for measuring brain temperature, both to characterize the extent to which temperature changes after stroke and to test protocols designed to reduce brain temperature. We used an animal model to test the ability of 1H MR spectroscopy to measure temperature from infarcted brain tissue at 24 hours after insult.


METHODS
Unilateral permanent focal ischemia in the middle cerebral artery territory was induced in adult dogs by intravascular delivery of microfibrillar collagen. MR imaging performed at 24 hours after insult was used to guide the implantation of temperature probes into the basal ganglia infarct and into the same anatomic location on the contralateral side. Serial non-water-suppressed 1H MR spectra were obtained from 1.3-cm3 voxels using an echo time of 136 and 272 ms, alternately, from the infarcted and contralateral non-infarcted tissue during a period when brain temperature was raised and lowered by whole-body heating and cooling.


RESULTS
The chemical shift difference between the 1H MR spectroscopy signal of water and N-acetylaspartate or water and trimethylamines was plotted against brain temperature for two voxel locations. The slope and intercept of the plots obtained for infarcted and non-infarcted brain were not significantly different (P < .05, t test), and there was no difference between the slope and intercept of plots made from data collected with an echo time of 136 or 272 ms.


CONCLUSIONS
The results of this study indicate that brain temperature can be measured from regions of brain containing infarcted tissue, at least up to 24 hours after ischemia. It should be possible to apply the 1H MR spectroscopy method used in the present study to measure brain temperature after stroke.
Corbett
R J
RJ
Department of Radiology, University of Texas Southwestern Medical Center at Dallas, 75235-9085, USA.
Purdy
P D
PD
Laptook
A R
AR
Chaney
C
C
Garcia
D
D
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001479:Basal Ganglia / Q000098:blood supply; D001812:Blood-Brain Barrier / Q000502:physiology; D001833:Body Temperature Regulation / Q000502:physiology*; D004285:Dogs; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis* / Q000503:physiopathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008744:Methylamines / Q000378:metabolism; D020768:Middle Cerebral Artery / Q000503:physiopathology
D008744:Methylamines; D001224:Aspartic Acid; C000179:N-acetylaspartate; C023336:trimethylamine