Microarray analysis of Drosophila development during metamorphosis.
Metamorphosis is an integrated set of developmental processes controlled by a transcriptional hierarchy that coordinates the action of hundreds of genes. In order to identify and analyze the expression of these genes, high-density DNA microarrays containing several thousand Drosophila melanogaster gene sequences were constructed. Many differentially expressed genes can be assigned to developmental pathways known to be active during metamorphosis, whereas others can be assigned to pathways not previously associated with metamorphosis. Additionally, many genes of unknown function were identified that may be involved in the control and execution of metamorphosis. The utility of this genome-based approach is demonstrated for studying a set of complex biological processes in a multicellular organism.
White
K P
KP
Department of Developmental Biology, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
Rifkin
S A
SA
Hurban
P
P
Hogness
D S
DS
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002490:Central Nervous System / Q000166:cytology / Q000254:growth & development; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004440:Ecdysone; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017344:Genes, Insect; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008675:Metamorphosis, Biological / Q000235:genetics*; D024510:Muscle Development; D009132:Muscles / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis
D004440:Ecdysone