Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue.
A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.
Manabe
Y C
YC
Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Chen
J M
JM
Ko
C G
CG
Chen
P
P
Bishai
W R
WR
D000581:Amidohydrolases / Q000235:genetics*; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D004790:Enzyme Induction / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D012808:Sigma Factor / Q000096:biosynthesis* / Q000235:genetics*; D015854:Up-Regulation
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C064971:FliA protein, Bacteria; D012808:Sigma Factor; C072681:Mycobacterium tuberculosis antigens; D000581:Amidohydrolases; C021445:acetamidase