title: Over-expression of cyclooxygenase-2 in human prostate adenocarcinoma.
abstract: Aberrant or increased expression of cyclooxygenase (COX)-2 has been implicated in the pathogenesis of many diseases including carcinogenesis. COX-2 has been shown to be over-expressed in some human cancers. Employing semi-quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry we assessed COX-2 expression in samples of pair-matched benign and cancer tissue obtained from the same prostate cancer patient. Mean levels of COX-2 mRNA were 3.4-fold higher in prostate cancer tissue (n = 12) compared with the paired benign tissue. The immunoblot analysis demonstrated that as compared to benign tissue COX-2 protein was over-expressed in 10 of 12 samples examined. Immunohistochemical analysis also verified COX-2 over-expression in cancer than in benign tissue. To our knowledge, this is the first in vivo study showing an over-expression of COX-2 in prostate cancer. These data suggest that COX-2 inhibitors may be useful for prevention or therapy of prostate cancer in humans.
authors.affiliation: Department of Dermatology, Case Western Reserve University Cleveland, Ohio 44106, USA.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: M
authors.initials: M
authors.lastname: Srivastava
authors.forename: N
authors.initials: N
authors.lastname: Ahmad
authors.forename: D G
authors.initials: DG
authors.lastname: Bostwick
authors.forename: H
authors.initials: H
authors.lastname: Mukhtar
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology*; D051546:Cyclooxygenase 2; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011467:Prostate / Q000201:enzymology; D011471:Prostatic Neoplasms / Q000201:enzymology*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases