Structural studies of cytoskeletal protein arrays formed on lipid monolayers.
Lipid monolayers have been widely used for the production of 2-D crystalline arrays of water-soluble proteins for structural analysis. Less well known is the utility of lipid layers for the assembly of multicomponent structures in two dimensions. This report summarizes current efforts and limitations to utilize a monolayer system composed of the quaternary ammonium surfactant didodecyldimethyammonium and dilaurylphosphatidylcholine to assemble 2-D complexes between actin and cytoskeletal proteins.
Taylor
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Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306-4380, USA. taylor@bio.fsu.edu
Taylor
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D000185:Actinin / Q000648:ultrastructure; D000199:Actins / Q000648:ultrastructure; D000818:Animals; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000648:ultrastructure*; D008055:Lipids / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods; D018482:Muscle, Skeletal; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D011817:Rabbits
D000199:Actins; D003598:Cytoskeletal Proteins; D008055:Lipids; D000644:Quaternary Ammonium Compounds; D000185:Actinin; C046112:didodecyldimethylammonium