title: Mutational analysis of avidity and fine specificity of anti-levan antibodies.
abstract: Using the polyfructose, bacterial levan, as a model polysaccharide, we analyzed how V regions affect binding in anti-polysaccharide mAbs. Previously, panels of mAb were constructed from bacterial levan-immunized BALB/c and CBA/Ca mice. The BALB/c mAb were mostly germline VHJ606:Vkappa11, and a subset contained presumed somatic mutations in the complementarity-determining regions (CDRs) that correlated with increases in avidity for the beta(2-->1) inulin linkage of levan. The CBA/Ca mAb were more heterogeneous in V gene usage, but a subset of inulin-nonreactive mAb were VHJ606:Vlambda and had VH sequence differences in the CDRs from the VHJ606 regions of the BALB/c mAb. In this report, VHJ606 Abs containing various combinations of specifically mutated H and L chains were produced by engineered transfectants and tested for inulin avidity and levan binding. Two presumed somatic mutations seen in CDRs of the BALB/c hybridomas were shown to directly cause marked increases in avidity for inulin (VH N53H, 9-fold; VL N53I, 20-fold; together, 46-fold) but not for beta(2-->6) levan. Exchange of either positions 50 or 53 in VH or the H3 loop between the BALB/c and CBA/Ca mAb resulted in either fine specificity shift or total loss of bacterial levan binding. Three-dimensional models of the V regions suggested that residues that affect binding to inulin alone are near the edge of the CDR surface, while residues involved with binding both forms of levan and affecting fine specificity are in the VH:VL junctional area.
authors.affiliation: Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA. Brorson@cber.fda.gov
authors.forename: K
authors.initials: K
authors.lastname: Brorson
authors.forename: C
authors.initials: C
authors.lastname: Thompson
authors.forename: G
authors.initials: G
authors.lastname: Wei
authors.forename: M
authors.initials: M
authors.lastname: Krasnokutsky
authors.forename: K E
authors.initials: KE
authors.lastname: Stein
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000915:Antibody Affinity / Q000235:genetics*; D000918:Antibody Specificity / Q000235:genetics*; D004252:DNA Mutational Analysis; D005630:Fructans / Q000276:immunology* / Q000378:metabolism; D006825:Hybridomas; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007146:Immunoglobulin lambda-Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007444:Inulin / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005630:Fructans; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D011135:Polysaccharides, Bacterial; D007444:Inulin; C072599:levan