The T3(b) gene promoter directs intestinal epithelial cell-specific expression in transgenic mice.
Although a few promoters that direct intestinal epithelial cell-specific expression in transgenic animals have been reported, they are not necessarily appropriate for transgenic studies in terms of activity and tissue specificity. Here, we examined the tissue specificity of transgene expression directed by the 2.8-kb promoter region of the T3(b) gene, which encodes one of the non-classical major histocompatibility complex class I molecules. The transgene was expressed exclusively in the epithelial cells of the small and large intestines at high levels. The results indicate that the T3(b) promoter is useful for directing transgene expression specifically in intestinal epithelial cells.
Aihara
H
H
Department of Nutrition and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, Japan.
Hiwatashi
N
N
Kumagai
S
S
Obata
Y
Y
Shimosegawa
T
T
Toyota
T
T
Miyazaki
J
J
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000378:metabolism; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D014018:Tissue Distribution
D010446:Peptide Fragments; D018664:Interleukin-12