A series of bioactivity-variant neurotoxins from scorpion Buthus martensii Karsch: X-ray crystal structure and functional implications.
Three bioactivity-variant neurotoxins, BmK M1, M4, and M8, have been purified from venom of the Chinese scorpion Buthus martensii Karsch. They possess distinct toxic activity on mice in vivo with different electrostatic properties. The relative toxicities of BmK M1, M4, and M8 are 13.3:2.5:1, which interestingly correspond to their respective pI values, ranging from basic to acidic, of 9.01, 7.53, and 5.30, respectively. In addition, the X-ray crystal structure of BmK M8, which is acidic and 1100 times less active than the most potent and basic alpha-toxin AaH II, have been determined at 1.85 A resolution and analyzed in detail. In combination with information from chemical modifications and site-directed mutagenesis, the structural comparisons and sequence alignments suggest a multisite binding mode for toxin-receptor interactions, and three "toxic regions" with relevance to the binding process, including Face A, Face B, and Site C. Face A is featured in the presence of several aromatic residues and may be more essential for the binding; Face B may contribute to the high efficacy of the binding process, on which substitution by acidic residues could weaken the toxic activity; Site C is probably involved in binding site specificity. The main residues involved in these regions will be discussed.
Wang
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Department of Protein Engineering, Chinese Academy of Sciences, Beijing, China.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019476:Insect Proteins; D007928:Lethal Dose 50; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009498:Neurotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity
C403888:BmK M1 neurotoxin; C403889:BmK M4 neurotoxin; C403890:BmK M8 neurotoxin; D019476:Insect Proteins; D009498:Neurotoxins; D012604:Scorpion Venoms