title: Nitric oxide and macrophage antiviral extrinsic activity.
abstract: In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. The extrinsic antiviral activity was observed in cultures of Vero cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-l-arginine acetate (l-NMA) impaired the antiviral activity of HSV-elicited macrophages. The effect was dose dependent and correlated with a reduction of nitrite in the culture media. The effect of l-NMA was reversed by the addition of l-arginine. These data indicate that NO could be responsible for the described activity. Furthermore, l-NMA treatment resulted in the aggravation of HSV-1-induced keratitis in the mouse model, supporting a defensive role of NO in the pathogenesis of HSV-1 corneal infection.
authors.affiliation: Department of Biological Chemistry, Faculty of Sciences, University of Buenos Aires, Ciudad Universitaria, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Benencia
authors.forename: M C
authors.initials: MC
authors.lastname: Courreges
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D005260:Female; D018259:Herpesvirus 1, Human; D018258:Herpesvirus 2, Human / Q000276:immunology*; D016849:Keratitis, Herpetic / Q000276:immunology* / Q000378:metabolism; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D014709:Vero Cells; D014779:Virus Replication / Q000276:immunology*; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D009573:Nitrites; D019323:omega-N-Methylarginine; D009569:Nitric Oxide