Rainbow trout (Oncorhynchus mykiss) possess two somatostatin mRNAs that are differentially expressed.
Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]-somatostatin (SS)-14 (now designated PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]-SS-14 (PPSS-II''). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II' and PPSS-II'' mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II" mRNA was present in brain. PPSS-II'' mRNA was more abundant than PPSS-II' mRNA in pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II" mRNA in stomach. Fasting increased pancreatic PPSS-II'' mRNA levels but had no effect on the levels of PPSS-II' mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.
Moore
C A
CA
Department of Zoology and Regulatory Biosciences Center, North Dakota State University, Fargo, North Dakota 58105, USA.
Kittilson
J D
JD
Ehrman
M M
MM
Sheridan
M A
MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009752:Nutritional Status; D017686:Oncorhynchus mykiss / Q000235:genetics*; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013004:Somatostatin / Q000235:genetics*; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D013004:Somatostatin