Effect of nitric oxide upon gas transfer and structural integrity of a polypropylene membrane oxygenator.
Gaseous nitric oxide (NO) may act as a membrane passivator during cardiopulmonary bypass by inhibition of platelet and leukocyte adhesion, activation, and aggregation. However, NO and its by-product nitrogen dioxide (NO2) are potently reactive and may be capable of degradation of membrane oxygenator constituents in an oxygen-rich environment. To test these concepts, nine polypropylene hollow fiber membrane oxygenators received 224 +/- 10 ppm NO and 6.7 +/- 1.7 ppm NO2 in 73% oxygen (O2), and six oxygenators received 73% O2, while being perfused with heparinized thrombocytopenic bovine blood for 6 hours. Oxygenators were used for measurement of O2 and carbon dioxide (CO2) transfer rates, structural integrity by pulsing with 22 psi water at 0.5 Hz for 6 hours, and scanning electron microscopic (SEM) examination of structural integrity. Transfer rates between groups at 0, 1, 3, and 6 hours revealed no differences in O2 or CO2. No oxygenator failed hydraulic tests of structural integrity or exhibited "wet-out" during bypass. No evidence of material degradation was shown in the SEM appearance of oxygenators. There were no differences in hematologic values. These data support the safety of gaseous NO in polypropylene membrane oxygenators for limited-term cardiopulmonary bypass.
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Department of Anesthesia, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
FitzGerald
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Voorhees
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Hansen
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Crowley
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Voorhees
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Shernan
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D000818:Animals; D001784:Blood Gas Analysis; D001798:Blood Proteins / Q000378:metabolism; D002245:Carbon Dioxide / Q000493:pharmacokinetics; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D001026:Coronary Artery Bypass / Q000295:instrumentation; D015199:Extracorporeal Membrane Oxygenation / Q000379:methods*; D007962:Leukocytes / Q000166:cytology; D008422:Materials Testing; D008567:Membranes, Artificial; D009569:Nitric Oxide / Q000494:pharmacology*; D010100:Oxygen / Q000493:pharmacokinetics; D010973:Platelet Adhesiveness / Q000187:drug effects; D011126:Polypropylenes; D011485:Protein Binding / Q000187:drug effects; D013552:Swine
D001798:Blood Proteins; D008567:Membranes, Artificial; D011126:Polypropylenes; D002245:Carbon Dioxide; D009569:Nitric Oxide; D010100:Oxygen