title: Occurrence of gamma-aminobutyric acid-transaminase activity in nerve fibers of human thymus.
abstract: The specific localization of gamma-aminobutyric acid-transaminase (GABA-t) in the thymus of young and elderly men was studied. Our results show a specific vascular localization of GABA-t in the human thymus, and deal with the amount and distribution of GABA-t and its changes with age. Samples of human thymus were harvested throughout of 12 autopsies in infants (n = 3), as well as young (n = 3), adult (n = 3) and elderly (n = 3) men. Histologic staining of the human thymus was performed with eosin-orange, while histologic staining of nerve fibers was performed with the Bodian method. Histochemical and biochemical demonstration of GABA-t, including protein dosage, was performed by the methods of Van Gelder and Jung, respectively. Finally, quantitative analysis of images was performed. Staining with eosin-orange reveals the micro-anatomical details of the thymic micro-environment. The Bodian method shows the nerve fibers and neurofibrils. Histochemical staining for GABA-t shows an increase of this enzyme with age and a marked localization in the nerve fibers of the thymus in infant, young, adult, and elderly men, as well as specific vascular localization of this enzyme. These biochemical data are in accordance with the histoenzymatic results and confirm all of our previous observations. Finally, quantitative analysis of images performed on slices let us confirm all the morphological changes induced by age. We can conclude that GABA is an inhibitory neurotransmitter of the human thymus, while GABA-t plays an important role in GABA metabolism.
authors.affiliation: Neurologic Clinic, Faculty of Pharmacy, University La Sapienza, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Cavallotti
authors.forename: M
authors.initials: M
authors.lastname: Artico
authors.forename: S
authors.initials: S
authors.lastname: De Santis
authors.forename: C
authors.initials: C
authors.lastname: Cavallotti
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology* / Q000294:innervation*; D014018:Tissue Distribution
chemical_list: D000612:4-Aminobutyrate Transaminase