Mapping of a gene causing mouse gutter-shaped tooth root to chromosome 5.
A study was conducted to identify the major candidate chromosome and to detect the region which included the candidate gene causing gutter-shaped root (GSR) in an inbred strain of mice. The candidate chromosomal analysis was performed on genetic crosses of C57L/J strain mice, which have GSR, and C57BL/6J strain mice, which have normal roots. Linkage analysis suggested that mouse chromosome 5 was one of the major candidates and therefore this chromosome was investigated in detail by individual genotyping of all backcross mice. The highest linkage was found at D5Mit161, and other high linkages were evident at D5Mit29, 321 and 427. Based on these findings, it is suggested that the gene causing GSR formation in mice maps close to these microsatellite loci.
Shimizu
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Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics; D005838:Genotype; D007178:Inbreeding; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D014071:Tooth Abnormalities / Q000235:genetics; D014092:Tooth Root / Q000002:abnormalities*