Statistics aided optimization for high-performance liquid chromatographic analysis of organic acids in tobacco.
HPLC analysis for organic acids in tobacco was optimized with the aid of statistical experimental design, a central composite face-centered design. In the design, only thirteen HPLC analyses were needed for identifying two optimal separation parameters. A Bio-Rad Aminex HPX-87H column was used for the analyses. An optimal separation for seven acids in tobacco was found at a temperature of 57 degrees C and a mobile phase of 0.032 N sulfuric acid solution, or at a temperature of 70 degrees C and a mobile phase of 0.024 N sulfuric acid solution, with a flow rate of 0.6 ml min(-1).
Qiu
J
J
United States Tobacco Manufacturing Limited Partnership, Nashville, TN 37203, USA. qiujinshu@yahoo.com
D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data*; D019343:Citric Acid / Q000032:analysis*; D008293:Malates / Q000032:analysis*; D010756:Phosphoric Acids / Q000032:analysis*; D010947:Plants, Toxic; D011761:Pyrrolidonecarboxylic Acid / Q000032:analysis*; D012997:Solvents; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*
D008293:Malates; D010756:Phosphoric Acids; D012997:Solvents; D019343:Citric Acid; C030298:malic acid; D011761:Pyrrolidonecarboxylic Acid