Requirement of yeast SGS1 and SRS2 genes for replication and transcription.
The SGS1 gene of the yeast Saccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion of SGS1 and SRS2 is lethal in yeast. Here, using a conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase I transcription are drastically inhibited in the srs2Delta sgs1-ts strain at the restrictive temperature. Thus, SGS1 and SRS2 function in DNA replication and RNA polymerase I transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.
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Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061, USA.
Johnson
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Yu
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Prakash
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Prakash
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D001816:Bloom Syndrome / Q000235:genetics; D003062:Codon; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D005800:Genes, Fungal; D006801:Humans; D009154:Mutation; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012345:RNA, Transfer, Amino Acid-Specific / Q000096:biosynthesis; D053484:RecQ Helicases; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic; D014898:Werner Syndrome / Q000235:genetics
D003062:Codon; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012345:RNA, Transfer, Amino Acid-Specific; D029701:Saccharomyces cerevisiae Proteins; C061376:SRS2 protein, S cerevisiae; D012319:RNA Polymerase II; D012318:RNA Polymerase I; D012320:RNA Polymerase III; C090472:SGS1 protein, S cerevisiae; D004265:DNA Helicases; D053484:RecQ Helicases