Macrocurrents of voltage gated Na+ and K+ channels from the crayfish stretch receptor neuronal soma.
Currents from the slowly adapting stretch receptor neuron of the crayfish (Pacifastacus leniusculus) were studied in a cell attached configuration using patch pipettes with an opening diameter of 2-10 microm. The neuronal membrane was enzymatically freed from the glial layer. The voltage gated Na+ and K+ channels seemed to be more concentrated in the lower part of soma close to the axon hillock. The Na+ and K+ currents could be analysed by fitting the currents to a fourth-order exponential function for Na+ current and a second-order exponential function for the K+ current. The macropatch recordings of enzymatically treated neurons are superior to two electrode voltage clamp recordings when analyzing voltage gated Na+ and K+ currents.
Lin
J H
JH
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Sand
P
P
Rydqvist
B
B
D000818:Animals; D003400:Astacoidea / Q000502:physiology; D015640:Ion Channel Gating; D008465:Mechanoreceptors / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D015222:Sodium Channels / Q000502:physiology*
D015221:Potassium Channels; D015222:Sodium Channels