1-Naphthol beta-D-glucuronide formed intraluminally in rat small intestine mucosa and absorbed into the colon.
UDP-glucuronosyltransferase expressed in the rat intestinal epithelial cells is important as the first barrier against chemicals. The distribution of 1-naphthol and its glucuronide formed in rat intestine was estimated by using everted intestine. Roughly 60% of the 1-naphthol added to the mucosal fluid was absorbed into the mucosa of the small intestine and colon within 30 min. Approximately 66% of the 1-naphthol absorbed in the proximal intestine was secreted intraluminally as a glucuronide, and a minimal 9% was transported into the serosal fluid as a glucuronide. In the distal intestine, approximately 34% was secreted intraluminally and 30% was transported into the serosal fluid as a glucuronide. The greatest amount of the glucuronide (37% of the absorbed 1-naphthol) was transported into the serosal fluid, whereas a minimal 7% was secreted intraluminally in the colon. In marked contrast, the colon was found to transport 1-naphthol-glucuronide from the mucosal fluid into the serosal fluid at an approximately 8-fold higher rate than that of the small intestine. These results suggest that, in the small intestine, phenolic xenobiotics are mostly glucuronidated and secreted intraluminally and that the resulting glucuronide is absorbed and transported into the serosal side of the colon.
Inoue
H
H
Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Yokota
H
H
Taniyama
H
H
Kuwahara
A
A
Ogawa
H
H
Kato
S
S
Yuasa
A
A
D000818:Animals; D001692:Biological Transport; D003106:Colon / Q000201:enzymology / Q000378:metabolism*; D004847:Epithelial Cells / Q000201:enzymology; D005965:Glucuronates; D020719:Glucuronides / Q000096:biosynthesis* / Q000378:metabolism / Q000493:pharmacokinetics; D014453:Glucuronosyltransferase / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007583:Jejunum / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D005965:Glucuronates; D020719:Glucuronides; D007527:Isoenzymes; C029091:naphthyl glucuronide; C110655:UDP-glucuronosyltransferase, UGT1A6; D014453:Glucuronosyltransferase; C107611:UDP-glucuronosyltransferase, UGT1A7