Disruption of TRI101, the gene encoding trichothecene 3-O-acetyltransferase, from Fusarium sporotrichioides.
We screened a Fusarium sporotrichioides NRRL 3299 cDNA expression library in a toxin-sensitive Saccharomyces cerevisiae strain lacking a functional PDR5 gene. Fourteen yeast transformants were identified as resistant to the trichothecene 4,15-diacetoxyscirpenol, and each carried a cDNA encoding the trichothecene 3-O-acetyltransferase that is the F. sporotrichioides homolog of the Fusarium graminearum TRI101 gene. Mutants of F. sporotrichioides NRRL 3299 produced by disruption of TRI101 were altered in their abilities to synthesize T-2 toxin and accumulated isotrichodermol and small amounts of 3, 15-didecalonectrin and 3-decalonectrin, trichothecenes that are not observed in cultures of the parent strain. Our results indicate that TRI101 converts isotrichodermol to isotrichodermin and is required for the biosynthesis of T-2 toxin.
McCormick
S P
SP
Mycotoxin Research Unit, USDA/ARS National Center for Agricultural Utilization Research, Peoria, Illinois 61604, USA. mccormsp@mail.ncaur.usda.gov
Alexander
N J
NJ
Trapp
S E
SE
Hohn
T M
TM
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D002474:Cell-Free System; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D015723:Gene Library; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae; D014169:Transformation, Bacterial; D014255:Trichothecenes / Q000378:metabolism
D018076:DNA, Complementary; D011994:Recombinant Proteins; D014255:Trichothecenes; D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase