Oxidative modification of serum albumin in an experimental glycation model of diabetes mellitus in vitro: effect of the pyridoindole antioxidant stobadine.
Under conditions of an experimental in vitro glycation model, the pyridoindole antioxidant stobadine significantly inhibited glycation-related fluorescence changes of bovine serum albumin as well as the yield of 2,4-dinitrophenylhydrazine-reactive carbonyls with an efficacy comparable to that of the reference antioxidants Trolox C and 2-keto-4-methiolbutyric acid, and more efficiently than did aminoguanidine. Since stobadine did not affect the covalent binding of glucose, the protective effect may be explained by the ability of the drug to eliminate free radical intermediates of glyco-oxidation reactions, operative after the preceding glycation steps.
Stefek
M
M
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Krizanova
L
L
Trnkova
Z
Z
D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D002243:Carbolines / Q000737:chemistry*; D005453:Fluorescence; D006031:Glycosylation; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000975:Antioxidants; D002243:Carbolines; D012710:Serum Albumin, Bovine; C100106:dicarbine