title: [Comparison of immunoassay kits for detection of staphylococcal enterotoxins produced by Staphylococcus aureus].
abstract: Four commercial kits, SET-RPLA, RIDASCREEN, SET-EIA, and TECRA, were compared for the efficiency of detecting staphylococcal enterotoxins (SE). There was no non-specific reaction for detection of SE produced by 21 Staphylococcus aureus strains from 5 outbreaks of food poisoning using SET-RPLA, SET-EIA and TECRA kits. The background of the results of RIDASCREEN kit was high and non-specific reactions were present in some strains.
  
  
  CONCLUSIONS
  (i) TECRA kit is suggested to be used for screening SE producing strains; (ii) SET-RPLA and RIDASCREEN kits are suitable for epidemiological investigation of SE types, but the lack of ability for detecting SEE, long time required for testing with SET-RPLA kit and high background when using RIDASCREEN kit must be overcome; and (iii) because of the complicated test procedures and the lack of ability for detecting SEE, the practicality of SET-EIA kit in screening and epidemiological research purposes is low.
authors.affiliation: National Institute of Preventive Medicine, Department of Health, Taipei, Taiwan, R.O.C.
authors.forename: T M
authors.initials: TM
authors.lastname: Pan
authors.forename: Y L
authors.initials: YL
authors.lastname: Yu
authors.forename: S I
authors.initials: SI
authors.lastname: Chiu
authors.forename: S S
authors.initials: SS
authors.lastname: Lin
mesh_terms: D004768:Enterotoxins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013202:Staphylococcal Food Poisoning / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000472:pathogenicity*
chemical_list: D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic