title: Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay.
abstract: Escherichia coli is the most common causative agent of urinary tract infection (UTI), and diagnosing this infection usually relies on bacteriologic methods. Nevertheless, screening methods can be useful for a rapid presumptive diagnosis even though some of these screening methods have low sensitivity or are expensive. To investigate a possible new alternative approach, an antigen-based immunoassay--enzyme-linked immunoelectrodiffusion assay (ELIEDA)--was standardized for screening for this bacterial infection. Combining counter-immunoelectrophoresis with an immunoenzymatic assay, the ELIEDA requires concentrated urine specimens, a cellulose acetate membrane, polyclonal antibodies to E. coli raised in rabbits, and peroxidase-labeled sheep antibodies to rabbit immunoglobulin G (IgG). This ELIEDA technique was evaluated using 244 urine specimens, 76 of them with E. coli, 47 with heterologous bacteria, and 121 without bacteria. In comparison to bacteriologic methods, the sensitivity, specificity, and positive and negative predictive values for the ELIEDA were 93.4%, 98.2%, 95.9%, and 97.1%, respectively. The data obtained suggest that this assay is useful for routine diagnostic screening for UTI caused by E. coli. In addition, since the ELIEDA stained membranes can be stored, this assay makes retrospective studies possible.
authors.affiliation: University of São Paulo, Department of Clinical and Toxicological Analysis, Brazil.
authors.forename: M T
authors.initials: MT
authors.lastname: Campanhã
authors.forename: S
authors.initials: S
authors.lastname: Hoshino-Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Baquerizo Martinez
mesh_terms: D000818:Animals; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000201:enzymology / Q000382:microbiology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000201:enzymology / Q000382:microbiology; D014556:Urine / Q000382:microbiology