Determinaton of two major metabolites of the novel anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid in hepatic microsomal incubations by high-performance liquid chromatography with fluorescence detection.
High-performance liquid chromatographic methods have been developed and validated for the glucuronidated and oxidative metabolites of the novel anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA), produced in human liver microsomal incubations. Calibration curves for DMXAA acyl glucuronide (DMXAA-Glu) and 6-hydroxymethyl-5-methylxanthenone-4-acetic acid (6-OH-MXAA) were constructed over the concentration ranges of 0.25 to 20 and 0.5 to 40 microM, respectively. Assay performance was determined by intra- and inter-day accuracy and precision of quality control (QC) samples. The difference between the theoretical and measured concentration, and the coefficient of variation, were less than 15% at low QC concentrations, and less than 10% at medium and high QC concentrations for both analytes. The methods presented good accuracy, precision and sensitivity for use in kinetic studies of the glucuronidated and oxidative metabolites of DMXAA in human liver microsomes.
Zhou
S
S
Department of Pharmacology and Clincial Pharmacology, The University of Auckland, New Zealand.
Paxton
J W
JW
Tingle
M D
MD
McCall
J
J
Kestell
P
P
D000970:Antineoplastic Agents / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D011786:Quality Control; D012680:Sensitivity and Specificity; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014966:Xanthenes / Q000378:metabolism*; D044004:Xanthones
D000970:Antineoplastic Agents; D006878:Hydroxides; D010756:Phosphoric Acids; D017680:Potassium Compounds; D014966:Xanthenes; D044004:Xanthones; C066668:vadimezan; D014535:Uridine Diphosphate Glucuronic Acid; D009249:NADP; C030242:phosphoric acid; C029943:potassium hydroxide