title: Reduction in bronchodilation following a deep inhalation is poorly related to airway inflammation in asthma.
abstract: In patients with bronchial asthma, forced expiratory flows are differently sensitive to a previous volume history. A reduced ability of a deep inhalation (DI) to dilate obstructed airways has been hypothesized to be a physiological marker for the degree of airway responsiveness and to relate to the presence and magnitude of inflammation in the lung, even in mild stable asthma. However, there are at present doubts as to whether functional changes could be used as a substitute for airway inflammation studies. In order to investigate the interrelations among airway inflammation, bronchial hyperresponsiveness and effects of volume history, 58 consecutive asthmatics with mild to moderate asthma were studied. The effects of DI were assessed as the isovolumic ratio of flows from forced expiratory manoeuvres started from maximal (M) or partial (P) lung inflation. Airway inflammation was assessed by using induced sputum. Sputum was analysed for total and differential cell counts, and levels of eosinophil cationic protein (ECP) which reflects eosinophil activation. Airway responsiveness was assessed as the provocative concentration of histamine which caused a 20% fall in forced expiratory volume in one second (FEV1) from control (PC20). The M/P ratio was significantly related to ECP (r=-0.31, p<0.03) and eosinophils (r=-0.29, p<0.03), FEV1/vital capacity (VC) (r=0.32; p<0.01), clinical score (r=-0.33; p<0.03) and age (r=-0.41; p<0.0001). In a stepwise multiple regression analysis including age, score, baseline lung function, ECP, number of eosinophils and the response to beta2-agonist, age (p<0.037) predicted a small amount of the variance in M/P ratio (r2=0.12). It is concluded that volume history response is substantially independent of both sputum outcomes (inflammatory cell number and eosinophil cationic protein) and bronchial hyperresponsiveness; rather it seems to be associated with anthropometric characteristics. Functional aspects do not provide information on eosinophilic, probably central, airway inflammation.
authors.affiliation: Fondazione Don C. Gnocchi ONLUS, Pozzolatico, Firenze, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Pacini
authors.forename: M
authors.initials: M
authors.lastname: Filippelli
authors.forename: R
authors.initials: R
authors.lastname: Duranti
authors.forename: E
authors.initials: E
authors.lastname: Rosi
authors.forename: I
authors.initials: I
authors.lastname: Romagnoli
authors.forename: M
authors.initials: M
authors.lastname: Grazzini
authors.forename: L
authors.initials: L
authors.lastname: Stendardi
authors.forename: G
authors.initials: G
authors.lastname: Misuri
authors.forename: G
authors.initials: G
authors.lastname: Scano
mesh_terms: D000328:Adult; D001249:Asthma / Q000503:physiopathology*; D001798:Blood Proteins / Q000032:analysis; D001980:Bronchi / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D003430:Cross-Sectional Studies; D047091:Eosinophil Granule Proteins; D004804:Eosinophils; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D007958:Leukocyte Count; D008176:Lung Volume Measurements; D008297:Male; D012119:Respiration; D012260:Ribonucleases; D013147:Spirometry; D013183:Sputum / Q000737:chemistry / Q000166:cytology
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D012260:Ribonucleases