Matrix protein of Chandipura virus inhibits transcription from an RNA polymerase II promoter.
Chandipura virus (CHPV) is a Vesiculovirus, related to, but phylogenetically distinct from, vesicular stomatitis virus (VSV). The matrix protein of VSV, as well as its role in virus assembly, inhibits the transcription from promoters for host RNA polymerases I and II. Cloning and expression of the matrix protein of CHPV in human cells showed that this protein is also functional in its inhibitory effect on transcription of a reporter gene from the cytomegalovirus immediate-early promoter, despite sharing only 28% amino acid sequence identity with the matrix protein of VSV.
Taylor
A
A
Department of Biological Sciences, University of Warwick, Coventry, UK.
Easton
A J
AJ
Marriott
A C
AC
D000595:Amino Acid Sequence; D002460:Cell Line; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014162:Transfection; D018116:Vesiculovirus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism
D014763:Viral Matrix Proteins; D012319:RNA Polymerase II