Assay method for the perfluorooctyl bromide (perflubron) in rat blood by gas chromatography-mass spectrometry.
This paper describes a GC-MS method (SIM mode) for the analysis of perfluorooctyl bromide (perflubron, I) in rat blood. The chromatographic separation was performed by injection in the split mode using a CP-select 624 CB capillary column. Following destruction of the emulsion by addition of ethanol, the analytical procedure involves a liquid-liquid extraction with 1,1,2-trichlorotrifluoroethane. The bis(F-butyl)ethene (II) was used as internal standard. Observed retention times were 3.22 min for I and 2.32 min for II. Two calibration curves were used; linear detection responses were obtained for concentrations ranging from 0.009 to 0.9 mg/ml and from 0.9 to 13.5 mg/ml. The extraction efficiency averaged 50% for I and 93% for II. Precision ranged from 0.7 to 14%, and accuracy was between 91 and 109%. The limit of quantification was 9 microg/ml. The method validation results indicate that the performance characteristics of the method fulfilled the requirements for assay method for use in pharmacokinetic studies.
Audran
M
M
Département de Biophysique, Faculté de Pharmacie, Université Montpellier 1, France.
Krafft
M P
MP
De Ceaurriz
J
J
Mathurin
J C
JC
Sicart
M T
MT
Marion
B
B
Fabre
F
F
Bressolle
F
F
D000818:Animals; D005466:Fluorocarbons / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006207:Half-Life; D006842:Hydrocarbons, Brominated; D007700:Kinetics; D008297:Male; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C003072:perflubron