Release of cytochrome c from mitochondria to cytosol in gerbil hippocampal CA1 neurons after transient forebrain ischemia.
We examined cytosolic cytochrome c in gerbil hippocampal CA1 and CA3 regions after induction of 5-min ischemia by immunoblotting. In the CA1 region, cytochrome c was detected in the cytosolic fraction from 1 to 6 h after ischemia by Western blotting, while it was not detected in the CA3 region. Following intraventricular administration of cyclosporin A (CsA), detectable cytosolic cytochrome c was dramatically decreased, and about 80% of CA1 neurons survived after ischemia. The present studies demonstrate that cytochrome c is translocated from mitochondria to the cytosol in the early stage of delayed neuronal cell death, and suggest the involvement of the mitochondrial permeability transition.
Nakatsuka
H
H
Department of Neurological Surgery, Ehime University School of Medicine, Shigenobu-cho, Onsen-gun, Ehime, 791-0295, Japan. hinakats@m.ehime-u.ac.jp
Ohta
S
S
Tanaka
J
J
Toku
K
K
Kumon
Y
Y
Maeda
N
N
Sakanaka
M
M
Sakaki
S
S
D000818:Animals; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007276:Injections, Intraventricular; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*
D016572:Cyclosporine; D003576:Electron Transport Complex IV