Lack of negative influence on the cellular transcription factors NF-kappaB and AP-1 by the nef protein of human immunodeficiency virus type 1.
In order to investigate the molecular mechanism of the reported negative effect of the Nef protein of human immunodeficiency virus type 1 (HIV-1) on the cellular transcription factors NF-kappaB and AP-1, human T cell lines (both populations and subclones) expressing the nef gene from HIV-1 clone pNL432 were constructed. Functional expression of the nef gene was confirmed by downregulation of CD4 and MHC class I proteins on the cell surface as measured by fluorescence-activated cell sorter analysis. However, contrary to previous reports, no significant difference was found in the induced level of NF-kappaB and AP-1 activity between nef(+) and nef(-) cell lines upon stimulation by phorbol 12-myristate 13-acetate and phytohaemagglutinin, as measured by transient transfection and electromobility shift assays. These data indicate that the Nef protein does not have a negative effect on the induction of NF-kappaB and AP-1.
Yoon
Keejung
K
Institute of Molecular Biology and Genetics, Building 105, Seoul National University, Kwan-Ak-Gu, Seoul 151-742, Korea1.
Kim
Sunyoung
S
Institute of Molecular Biology and Genetics, Building 105, Seoul National University, Kwan-Ak-Gu, Seoul 151-742, Korea1.
D015704:CD4 Antigens / Q000032:analysis; D002460:Cell Line; D015693:Gene Products, nef / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D016328:NF-kappa B / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000502:physiology*; D054311:nef Gene Products, Human Immunodeficiency Virus
D015704:CD4 Antigens; D015693:Gene Products, nef; D015395:Histocompatibility Antigens Class I; D016328:NF-kappa B; D010835:Phytohemagglutinins; D018808:Transcription Factor AP-1; D054311:nef Gene Products, Human Immunodeficiency Virus; D013755:Tetradecanoylphorbol Acetate