Fast isolation of recombinant baculovirus by antibody screening.
We describe a rapid and efficient scheme for the isolation and purification of recombinant baculoviruses. The method is based on the detection of foreign proteins in cellular lysates of baculovirus-infected insect cells by antibody screening. The recombinant virus is purified by repeated serial dilutions. The method allows the identification and purification of recombinant viruses within 2 to 3 wk. This procedure selects for recombinant baculoviruses that highly overproduce the desired protein product.
Grosse
F
F
Institut für Molekulare Biotechnologie, Abt. Biochemie, Jena, Germany.
Manns
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D016367:Baculoviridae / Q000302:isolation & purification*; D002460:Cell Line; D007313:Insecta / Q000821:virology; D008828:Microbiological Techniques; D016865:Reassortant Viruses / Q000302:isolation & purification; D011994:Recombinant Proteins
D000914:Antibodies, Viral; D011994:Recombinant Proteins