Mutant mice lacking Crk-II caused by the gene trap insertional mutagenesis: Crk-II is not essential for embryonic development.
Crk family adapter proteins including Crk-II, Crk-I, and Crk-L consist mostly of SH2 and SH3 domains. Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline-rich motifs, they are involved in a variety of signaling cascades. Despite their essential roles in the signal transductions, knock-out mice of these molecules have not been reported yet. We performed the gene trap insertional mutagenesis with a trap vector, pU-Hachi, and generated a mutant mice line, Ayu 8104, in which the trap vector was inserted into the c-crk gene. Homozygous Ayu 8104 mice lacked Crk-II and Crk-I transcripts but expressed the truncated Crk proteins retaining one SH2 and one SH3 domain. Since the structure of the truncated proteins was similar to that of Crk-I, the insertion was considered to cause Crk-II-specific disruption. Homozygous mutant mice, however, did not exhibit any obvious abnormalities, suggesting that Crk-family adapters, Crk-II, Crk-I, and Crk-L would redundantly function in the signaling cascades and Crk-II was not apparently essential for embryonic development.
Imaizumi
T
T
Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kuhonji 4-24-1, Kumamoto, 862-0976, Japan.
Araki
K
K
Miura
K
K
Araki
M
M
Suzuki
M
M
Terasaki
H
H
Yamamura
K
K
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D005314:Embryonic and Fetal Development / Q000235:genetics*; D007668:Kidney / Q000737:chemistry; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics; D018909:src Homology Domains / Q000235:genetics
D011518:Proto-Oncogene Proteins; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger