title: HDL-subpopulation patterns in response to reductions in dietary total and saturated fat intakes in healthy subjects.
abstract: BACKGROUND
  Little information is available about HDL subpopulations during dietary changes.
  
  
  OBJECTIVE
  The objective was to investigate the effect of reductions in total and saturated fat intakes on HDL subpopulations.
  
  
  METHODS
  Multiracial, young and elderly men and women (n = 103) participating in the double-blind, randomized DELTA (Dietary Effects on Lipoproteins and Thrombogenic Activities) Study consumed 3 different diets, each for 8 wk: an average American diet (AAD: 34.3% total fat,15.0% saturated fat), the American Heart Association Step I diet (28.6% total fat, 9.0% saturated fat), and a diet low in saturated fat (25.3% total fat, 6.1% saturated fat).
  
  
  RESULTS
  HDL(2)-cholesterol concentrations, by differential precipitation, decreased (P < 0.001) in a stepwise fashion after the reduction of total and saturated fat: 0.58 +/- 0.21, 0.53 +/- 0.19, and 0.48 +/- 0.18 mmol/L with the AAD, Step I, and low-fat diets, respectively. HDL(3) cholesterol decreased (P < 0.01) less: 0.76 +/- 0.13, 0.73 +/- 0.12, and 0.72 +/- 0.11 mmol/L with the AAD, Step I, and low-fat diets, respectively. As measured by nondenaturing gradient gel electrophoresis, the larger-size HDL(2b) subpopulation decreased with the reduction in dietary fat, and a corresponding relative increase was seen for the smaller-sized HDL(3a, 3b), and (3c) subpopulations (P < 0.01). HDL(2)-cholesterol concentrations correlated negatively with serum triacylglycerol concentrations on all 3 diets: r = -0.46, -0.37, and -0.45 with the AAD, Step I, and low-fat diets, respectively (P < 0.0001). A similar negative correlation was seen for HDL(2b), whereas HDL(3a, 3b), and (3c) correlated positively with triacylglycerol concentrations. Diet-induced changes in serum triacylglycerol were negatively correlated with changes in HDL(2) and HDL(2b) cholesterol.
  
  
  CONCLUSIONS
  A reduction in dietary total and saturated fat decreased both large (HDL(2) and HDL(2b)) and small, dense HDL subpopulations, although decreases in HDL(2) and HDL(2b) were most pronounced.
authors.affiliation: Department of Medicine and Pediatrics, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA. berglun@cudept.cis.columbia.edu
authors.forename: L
authors.initials: L
authors.lastname: Berglund
authors.forename: E H
authors.initials: EH
authors.lastname: Oliver
authors.forename: N
authors.initials: N
authors.lastname: Fontanez
authors.forename: S
authors.initials: S
authors.lastname: Holleran
authors.forename: K
authors.initials: K
authors.lastname: Matthews
authors.forename: P S
authors.initials: PS
authors.lastname: Roheim
authors.forename: H N
authors.initials: HN
authors.lastname: Ginsberg
authors.forename: R
authors.initials: R
authors.lastname: Ramakrishnan
authors.forename: M
authors.initials: M
authors.lastname: Lefevre
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D044383:Black People; D002318:Cardiovascular Diseases / Q000517:prevention & control*; D008076:Cholesterol, HDL / Q000097:blood* / Q000145:classification / Q000187:drug effects; D018592:Cross-Over Studies; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood; D014481:United States; D044465:White People; D001741:Black or African American
chemical_list: D008076:Cholesterol, HDL; D004041:Dietary Fats; D014280:Triglycerides