title: Effect of laser welding with human serum albumin on the expression of P-selectin on platelets.
abstract: OBJECTIVE
  Artery repair by means of laser energy induces activation of platelets with a risk of thrombosis and local inflammatory reactions. The aim of this study was to investigate the effect of human serum albumin, the most common solder in laser surgery, on platelet activation.
  
  
  METHODS
  Platelet activation was evaluated in canine blood by using two-color flow cytometry with a phycoerythrin-labeled antibody to a common platelet marker, glycoprotein IIb/IIIa and fluorescein isothiocyanate-labeled antibody to a platelet activation molecule, P-selectin. Human serum albumin was applied in vitro and in vivo, as a solder during laser reconstruction of canine arteries.
  
  
  RESULTS
  In vitro, albumin significantly (P < 0.01) reduces the expression of P-selectin on platelets. This is most likely related to the blockage of P-selectin by albumin, which binds to the platelet surface, as confirmed by flow cytometry with fluorescein isothiocyanate-labeled albumin. In vivo, application of albumin solder tended to result in a lower percentage of P-selectin-expressing platelets in laser-repaired arteries compared to suture-repaired arteries.
  
  
  CONCLUSIONS
  Albumin decreases the percentage of P-selectin-expressing platelets in vitro. Further research may allow the platelet activation inhibiting properties of albumin to be further optimized in vivo.
authors.affiliation: The Center for Pediatric Urology and Laboratory for Minimally Invasive Urologic Surgery, Department of Urology, New York Presbyterian Hospital, Weill Medical College of Cornell University, New York, NY 10021, USA.
authors.forename: I
authors.initials: I
authors.lastname: Kirman
authors.forename: A
authors.initials: A
authors.lastname: Lauto
authors.forename: A
authors.initials: A
authors.lastname: Phillips
authors.forename: A
authors.initials: A
authors.lastname: Hamawy
authors.forename: E
authors.initials: E
authors.lastname: Heldman
authors.forename: B
authors.initials: B
authors.lastname: Cuomo
authors.forename: S J
authors.initials: SJ
authors.lastname: Shin
authors.forename: R
authors.initials: R
authors.lastname: Soslow
authors.forename: D
authors.initials: D
authors.lastname: Felsen
authors.forename: D P
authors.initials: DP
authors.lastname: Poppas
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002339:Carotid Arteries / Q000601:surgery*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D066298:In Vitro Techniques; D053685:Laser Therapy / Q000379:methods*; D019007:P-Selectin / Q000097:blood* / Q000187:drug effects; D015539:Platelet Activation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000187:drug effects / Q000378:metabolism; D012709:Serum Albumin / Q000494:pharmacology*; D014014:Tissue Adhesives / Q000494:pharmacology*
chemical_list: D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D012709:Serum Albumin; D014014:Tissue Adhesives