Phosphatidylserine-mediated phagocytosis of anticancer drug-treated cells by macrophages.
Apoptotic cells are rapidly phagocytosed and eliminated from the organism. Although cancer cells apoptose when treated with anticancer drugs, how those cells are recognized by phagocytic cells has remained unclear. The human leukemia cell line Jurkat was cultured with doxorubicin or bufalin and induced to undergo apoptosis accompanied by phosphatidylserine externalization. When apoptotic Jurkat cells were mixed with mouse peritoneal macrophages, efficient phagocytosis was observed. Apoptosis and phagocytosis of Jurkat cells were both inhibited by Z-VAD-FMK, and phagocytosis was significantly reduced in the presence of phosphatidylserine-containing liposomes. These results suggest that anticancer drugs induce apoptosis-dependent and phosphatidylserine-mediated phagocytosis in cancer cells.
Shiratsuchi
A
A
Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa, 920-0934, Japan.
Nakanishi
Y
Y
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D005260:Female; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008081:Liposomes / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D010718:Phosphatidylserines / Q000378:metabolism*
D000590:Amino Acid Chloromethyl Ketones; D000970:Antineoplastic Agents; D008081:Liposomes; D010718:Phosphatidylserines; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone