Interactive computerized microscopy as a tool for quantifying vascular remodelling effects of diabetes and V1a receptor antagonist SR 49059 on rat mesenteric arterial bed.
A methodology using interactive computerized microscopy (ICM) was developed to quantify in the mesenteric arterial bed the morphometric changes associated with diabetes and the influence of treatment with SR 49059, an antagonist of vasopressin V1a receptors. Four groups of rats were studied: untreated normal (N) or streptozotocin- (60 mg/kg i.v.) induced diabetic (D), and treated (0.4 mg/g SR 49059 included in food) normal (NT) or diabetic (DT) animals. Treatment was initiated 4 days after diabetes induction and continued for 3 weeks. Nested (hierarchical) analysis of variance of ICM data was performed on raw diameter or after logarithmic normalization of area and nuclei values. Diabetes was associated with an increase in arterial diameters, and in total vessel, wall, media, adventitia, and lumen areas. The same parameters, with the exception of the lumen, were also increased in DT as compared to D. The number of nuclei in the media or adventitia was increased in D as compared to N, and in DT as compared to D. In summary, ICM is allowed to further characterize the vascular mesenteric changes and describe for the first time the enlargement of adventitia associated with diabetes. Our study also suggested that the blockade of Via receptors is unable to prevent diabetes-related vascular changes, although the slight increase in food intake associated with SR 49059 treatment may have had an indirect influence on angiopathy development.
Méchaly
I
I
Laboratoire de Pharmacologie et Physiopathologie Expérimentales, Faculté de Pharmacie, Montpellier, France.
Krosniak
M
M
Azay
J
J
Cassanas
G
G
Roque
C
C
Cahard
D
D
Serrano
J J
JJ
Cros
G
G
D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001786:Blood Glucose; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D004326:Drinking; D004435:Eating / Q000187:drug effects; D006727:Hormone Antagonists / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D008638:Mesenteric Arteries / Q000473:pathology / Q000502:physiology*; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000187:drug effects*
D065092:Antidiuretic Hormone Receptor Antagonists; D001786:Blood Glucose; D006727:Hormone Antagonists; D007211:Indoles; D011759:Pyrrolidines; C082134:relcovaptan