Characterization of a Mycobacterium smegmatis mutant lacking penicillin binding protein 1.
The ponA gene of Mycobacterium smegmatis encodes a 95-kDa penicillin binding protein, PBP1, that is similar to PBP1s of Mycobacterium tuberculosis and Mycobacterium leprae. Transposon disruption of ponA in M. smegmatis resulted in a PBP1-deficient mutant that was sensitive to beta-lactam antibiotics, was more permeable to glycine, and grew slowly in liquid culture.
Billman-Jacobe
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Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia. Helen.BJ@med.monash.edu.au
Haites
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Coppel
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D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics*; D002463:Cell Membrane Permeability / Q000235:genetics; D003470:Culture Media; D005998:Glycine / Q000378:metabolism; D006602:Hexosyltransferases / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D020102:Mycobacterium smegmatis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001618:beta-Lactamases; D005998:Glycine