A model of cochlear function assessment during reversible ischemia in the Mongolian gerbil.
An in vivo model in the Mongolian gerbil is used to assess the auditory changes during reversible cochlear ischemia. This model allows the recordings of cochlear potentials (microphonics, summating potential and compound action potential) and otoacoustic emissions (cubic difference tones, CDTs), together with laser Doppler cochlear blood flow (CBF) measurements, over reversible cochlear ischemia. Ischemia is achieved by compression of the eighth nerve complex at the porus of the internal acoustical meatus, whereas the compression release allows the reperfusion to occur. While CBF monitoring permits to objectively determine the degree of ischemia and reperfusion, the combined analysis of cochlear potentials and CDTs makes it possible to point out the preferential site of main functional damage within the cochlea, i.e., outer hair cells (OHCs), inner hair cells, or ganglia cells. At least three ischemia-reperfusion sequences can be used on one side, and sometimes both ears can serve for experiments. This model could be applicable for testing drugs with alleged protective effects against cochlear ischemia and/or reperfusion, in treated vs. non-treated animals.
Mom
T
T
Service d'Otorhinolaryngologie et Laboratoire de Biophysique Sensorielle, Centre Hospitalier Universitaire, Université D'Auvergne, BP 69, 63003, Clermont-Ferrand, France. tmom@chuclermontferrand.fr
Avan
P
P
Bonfils
P
P
Gilain
L
L
D000818:Animals; D001306:Auditory Pathways / Q000098:blood supply / Q000502:physiology; D002530:Cerebellopontine Angle / Q000601:surgery; D003051:Cochlea / Q000098:blood supply* / Q000502:physiology* / Q000601:surgery; D004195:Disease Models, Animal; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry; D008564:Membrane Potentials / Q000502:physiology; D015427:Reperfusion Injury / Q000503:physiopathology