title: Production of growth factors by in vitro cultured human endothelial cells after contact with carbon coated polyethylene terephthalate.
abstract: The release of basic fibroblast growth factor (bFGF) and platelet derived growth factor AB (PDGF-AB) by in vitro cultured human umbilical endothelial cells in contact with carbon and collagen coated polyethylene terephthalate (PET + PC) was assessed by enzyme immunoassay. The same cells cultured on polystyrene without biomaterials were tested as negative control. PET + PC induced a significant increase in the release of bFGF after 72 h and a significative reduction in the release of PDGF-AB after 48 and 72 h, compared to the negative control.
authors.affiliation: Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: A
authors.initials: A
authors.lastname: Corradini
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
authors.forename: L
authors.initials: L
authors.lastname: Montanaro
mesh_terms: D001672:Biocompatible Materials; D002244:Carbon; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000378:metabolism; D011093:Polyethylene Terephthalates; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D010982:Platelet-Derived Growth Factor; D011093:Polyethylene Terephthalates; D016222:Fibroblast Growth Factor 2; D002244:Carbon; D003094:Collagen