title: A copper-sensing transcription factor regulates iron uptake genes in Schizosaccharomyces pombe.
abstract: Copper and iron serve essential functions as catalytic co-factors in a wide variety of critical cellular enzymes. Studies in yeast have demonstrated an absolute dependence upon copper acquisition for proper assembly and function of the iron transport machinery. We have cloned genes for a high affinity copper transporter (Ctr4) and copper-sensing transcription factor (Cuf1) from Schizosaccharomyces pombe. Interestingly, the primary structure of Ctr4 and a putative human high affinity copper transport protein, hCtr1, suggests that they are derived from a fusion of the functionally redundant but structurally distinct Ctr1 and Ctr3 copper transporters from Saccharomyces cerevisiae. Furthermore, although Cuf1 activates ctr4(+) gene expression under copper starvation conditions, under these same conditions Cuf1 directly represses expression of genes encoding components of the iron transport machinery. These studies have identified an evolutionary step in which copper transport modules have been fused, and describe a mechanism by which a copper-sensing factor directly represses expression of the iron uptake genes under conditions in which the essential copper co-factor is scarce.
authors.affiliation: Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0606, USA.
authors.forename: S
authors.initials: S
authors.lastname: Labbé
authors.forename: M M
authors.initials: MM
authors.lastname: Peña
authors.forename: A R
authors.initials: AR
authors.lastname: Fernandes
authors.forename: D J
authors.initials: DJ
authors.lastname: Thiele
mesh_terms: D017920:Antiporters / Q000378:metabolism; D027682:Cation Transport Proteins; D003300:Copper / Q000378:metabolism*; D000079923:Copper Transporter 1; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D000080083:SLC31 Proteins; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D017920:Antiporters; C490078:CTR1 protein, S cerevisiae; C100982:CTR3 protein, S cerevisiae; D027682:Cation Transport Proteins; D000079923:Copper Transporter 1; D005656:Fungal Proteins; D008565:Membrane Proteins; D000080083:SLC31 Proteins; C490079:SLC31A1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003300:Copper; D007501:Iron