Enhanced metastasis after local therapy in a murine model using C-1300 neuroblastoma.
OBJECTIVE
In the treatment of advanced neuroblastoma, the role of surgery has been controversial. This study was carried out to determine the effect of surgery, irradiation, and chemotherapy in inhibiting or promoting distant metastases.


METHODS
Transplanted C-1300 neuroblastomas in hind legs of syngeneic mice were treated by surgery, radiation, and chemotherapy. The liver was evaluated 18 days after each treatment modality for metastases.


RESULTS
Mice developed no liver metastasis when leg tumors received no treatment or chemotherapy. In the mice who had the tumor resected, liver metastases were found in 8 of 16 mice that had 7-mm tumors. One hundred percent of the mice that had 9- or 12-mm tumors presented with metastases to the liver. In mice who received radiation therapy, 100% had liver metastases.


CONCLUSIONS
Local control by surgery and single-dose radiation induced liver metastasis in a murine model. Surgery to remove tumors should be used in conjunction with chemotherapy to prevent secondary liver metastases.
Iwakawa
M
M
Department of Pediatric Surgery, Institute of Clinical Medicine, University of Tsukuba, Ibaraki-ken, Japan.
Kaneko
M
M
Ikebulkuro
K
K
D000671:Amputation, Surgical / Q000009:adverse effects; D000818:Animals; D001859:Bone Neoplasms / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D003520:Cyclophosphamide / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D015994:Incidence; D008113:Liver Neoplasms / Q000453:epidemiology / Q000209:etiology / Q000556:secondary*; D051379:Mice; D008805:Mice, Inbred A; D009447:Neuroblastoma / Q000401:mortality / Q000556:secondary* / Q000628:therapy*; D011878:Radiotherapy / Q000009:adverse effects; D018570:Risk Assessment; D012720:Severity of Illness Index; D016019:Survival Analysis
D003520:Cyclophosphamide