title: gammadelta T cells regulate mucosally induced tolerance in a dose-dependent fashion.
abstract: We used gammadelta TCR-deficient (TCRdelta(-/-)) mice to examine the role of gammadelta T cells for induction of mucosal responses and systemic tolerance to high versus low doses of oral antigen. When either TCRdelta(-/-) or TCRdelta(+/+) mice were immunized orally with a high dose of ovalbumin (OVA) prior to parenteral challenge, systemic IgG and IgE antibody responses were markedly reduced in both types of mice, while mucosal IgA responses were reduced only in the TCRdelta(-/-) mice. Reduced T cell proliferative responses and delayed-type hypersensitivity were seen in TCRdelta(-/-) and TCRdelta(+/+) mice given the high dose of OVA. Antigen-induced T(h)1 and T(h)2 cytokine production by splenic CD4(+) T cells was severely inhibited in orally tolerized TCRdelta(-/-) and TCRdelta(+/+) mice. In contrast, while oral tolerance associated with increased levels of IL-10 synthesis was induced by a low dose of OVA in TCRdelta(+/+) mice, the TCRdelta(-/-) mice were not tolerized and failed to produce IL-10. Our findings indicate that gammadelta T cells play a significant immunoregulatory role in IL-10-mediated, low-dose oral tolerance induction, but are not essential participants in the induction of systemic tolerance to orally introduced antigens given in larger doses.
authors.affiliation: Department of Oral Biology, Immunobiology Vaccine Center, University of Alabama at Birmingham, Medical Center, Birmingham, AL 35294, USA.
authors.forename: K
authors.initials: K
authors.lastname: Fujihashi
authors.forename: T
authors.initials: T
authors.lastname: Dohi
authors.forename: M N
authors.initials: MN
authors.lastname: Kweon
authors.forename: J R
authors.initials: JR
authors.lastname: McGhee
authors.forename: T
authors.initials: T
authors.lastname: Koga
authors.forename: M D
authors.initials: MD
authors.lastname: Cooper
authors.forename: S
authors.initials: S
authors.lastname: Tonegawa
authors.forename: H
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D006968:Hypersensitivity, Delayed / Q000517:prevention & control; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D007114:Immunization; D007070:Immunoglobulin A / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016207:Cytokines; D007070:Immunoglobulin A; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016753:Interleukin-10; D010047:Ovalbumin