Sequence analysis of ARI-1, a novel OXA beta-lactamase, responsible for imipenem resistance in Acinetobacter baumannii 6B92.
The sequence of the bla(ARI-1) gene from imipenem-resistant Acinetobacter baumannii 6B92 has been determined. The structural gene encodes a 273-amino-acid protein which is most related to the OXA class D beta-lactamases. The conserved S-T-F-K and K-T-G motifs were identified in the ARI-1 protein sequence, also named OXA-23, but significantly, a point mutation (Y-->F) was identified in the Y-G-N conserved motif, also known to function in the active site.
Donald
H M
HM
Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, United Kingdom.
Scaife
W
W
Amyes
S G
SG
Young
H K
HK
D000150:Acinetobacter / Q000145:classification / Q000187:drug effects* / Q000201:enzymology; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D015378:Imipenem / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013845:Thienamycins / Q000494:pharmacology*; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics*
D001426:Bacterial Proteins; D013845:Thienamycins; D015378:Imipenem; D001618:beta-Lactamases; C000599728:beta-lactamase OXA-23, Acinetobacter baumannii