A quantitative immunocytochemical approach to the analysis of type I cells in the cat carotid body.
Digital image analysis of immunostained semithin plastic sections indicates that experimentally induced changes in levels of transmitter-related reaction product in single cells fails to support the concept of clearly defined subsets of type I cells in the carotid body. This objective approach to the quantitation of staining product on a cell-by-cell basis appears to indicate that the observed changes are related to global shifts in the expression of a given neuronal marker throughout a single population of highly labile chemoreceptor elements.
Major
J
J
Department of Physiology, University of Utah School of Medicine, Salt Lake City, UT 84108,USA.
Dinger
B
B
Stensaas
L J
LJ
Wang
Z Z
ZZ
D000818:Animals; D002344:Carotid Body / Q000166:cytology* / Q000378:metabolism*; D002415:Cats; D003714:Denervation; D005260:Female; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D013194:Staining and Labeling; D013373:Substance P / Q000378:metabolism; D013562:Sympathectomy; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D013373:Substance P; D014446:Tyrosine 3-Monooxygenase