Pyrolysis-gas-liquid chromatography with atomic emission detection for the identification of Corynebacterium species.
We report here the application of pyrolysis-gas chromatography followed by atomic emission detection (AED) for the characterisation of microorganisms. AED measured the quantity of carbon, sulfur and nitrogen in the molecules separated chromatographically. Twenty-three strains, representing eight Corynebacterium species, were tested in this preliminary study. Co-ordinate principal analysis grouped 11 strains in their respective species group. Most of the other strains appear randomly distributed, perhaps because these strains require additional nutrients. These preliminary results show that the method could be used as a tool for the taxonomic and perhaps the epidemiologic characterisation of bacteria.
Voisin
S
S
Département de Pharmacie Clinique, de Pharmacocinétique et d'Evaluation du Médicament, Institut des Sciences Pharmaceutiques et Biologiques, Université Claude Bernard Lyon 1, France. sebvoisin@hotmail.com
Renaud
F N
FN
Freney
J
J
de Montclos
M
M
Boulieu
R
R
Deruaz
D
D
D002244:Carbon / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification*; D006358:Hot Temperature; D009584:Nitrogen / Q000032:analysis; D013455:Sulfur / Q000032:analysis
D013455:Sulfur; D002244:Carbon; D009584:Nitrogen