Isolation of Bacteroides fragilis mutants with in vivo growth defects by using Tn4400', a modified Tn4400 transposition system, and a new screening method.
A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich medium anaerobically.
Tang
Y P
YP
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Malamy
M H
MH
D000818:Animals; D001441:Bacteroides fragilis / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D002460:Cell Line; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D014774:Virulence / Q000235:genetics
D004251:DNA Transposable Elements