Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice.
The growth and spread of tumour cells depends on adequate vasculature. We have previously reported that the expression of interleukin-8 (IL-8) directly correlates with the vascularity of human gastric carcinomas. To provide evidence for a causal role of IL-8 in angiogenesis and tumorigenicity of human gastric cancer, we used the lipofectin method to stably transfect the human TMK-1 gastric carcinoma cells (low endogenous IL-8) with an IL-8 expression vector or control vector. Transfection with IL-8 did not affect the proliferation of cultured cells, yet the culture supernatants of the transfected (but not control) cells stimulated proliferation of human umbilical vein endothelial cells. The IL-8-transfected and control cells were injected into the gastric wall of nude mice. IL-8-transfected cells produced rapidly growing, highly vascular neoplasms as compared to control cells. These results provide direct evidence for the role of IL-8 in the angiogenesis and tumorigenicity of human gastric carcinomas.
Kitadai
Y
Y
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Takahashi
Y
Y
Haruma
K
K
Naka
K
K
Sumii
K
K
Yokozaki
H
H
Yasui
W
W
Mukaida
N
N
Ohmoto
Y
Y
Kajiyama
G
G
Fidler
I J
IJ
Tahara
E
E
D000818:Animals; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000209:etiology*; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000209:etiology / Q000473:pathology; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D016209:Interleukin-8