title: Assembly of the epstein-barr virus BBLF4, BSLF1 and BBLF2/3 proteins and their interactive properties.
abstract: Epstein-Barr virus (EBV) encodes putative helicase-primase proteins BBLF4, BSLF1 and BBLF2/3, which are essential for the lytic phase of viral DNA replication. The BSLF1, BBLF4 and BBLF2/3 proteins were expressed in B95-8 cells after induction of a virus productive cycle, possessing apparent molecular masses of 89 kDa, 90 kDa and 80 kDa, respectively. The anti-BSLF1 or anti-BBLF2/3 protein-specific antibody, which recognizes its target protein in both Western blotting and immunoprecipitation analyses, immunoprecipitated all of the BSLF1, BBLF4 and BBLF2/3 proteins from the extract of the cells with a virus productive cycle, indicating that these viral proteins are assembled together in vivo. To characterize their protein-protein interactions in detail, recombinant baculoviruses capable of expressing each of these viral gene products in insect cells were constructed. The assembly of the three virus replication proteins was reproduced in insect cells co- infected with the three recombinant baculoviruses, indicating that complex formation does not require other EBV replication proteins. Furthermore, experiments performed by using the extracts from insect cells co-infected with each pair of the recombinant viruses demonstrated that the BSLF1 protein could interact separately with both the BBLF4 and BBLF2/3 proteins and that the BBLF2/3 protein also interacted with the BBLF4 protein. These observations strongly suggest that within the BBLF4-BSLF1-BBLF2/3 complex each component interacts directly with the other two, resulting in helicase-primase enzyme activity.
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Ken
authors.initials: K
authors.lastname: Fujii
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Mineo
authors.initials: M
authors.lastname: Hirata
authors.affiliation: Research and Development Department, Medical and Biological Laboratories, Ohara, Terasawaoka, Ina 396-0002, Japan2.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Tamai
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kiyono
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kuzushima
authors.affiliation: Laboratory of Virology, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan3.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishiyama
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujita
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan1.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tsurumi
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D004265:DNA Helicases / Q000502:physiology*; D019915:DNA Primase / Q000502:physiology*; D004261:DNA Replication; D004854:Herpesvirus 4, Human / Q000502:physiology*; D011817:Rabbits; D018411:Spodoptera; D014764:Viral Proteins / Q000502:physiology*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D019915:DNA Primase; D004265:DNA Helicases