Simultaneous core 2 beta1-->6N-acetylglucosaminyltransferase up-regulation and sialyl-Le(X) expression during activation of human tonsillar B lymphocytes.
We have investigated the regulation mechanism of the surface sialyl-Le(X) (sLe(X)) expression level in tonsillar B cells during activation. sLe(X) antigen became strongly positive after activation, while resting B cells were weakly positive. sLe(X) structures were mainly located on O-linked oligosaccharide chains of glycoprotein. Transcripts of FucT-VII and core 2 GlcNAc transferase (C2GnT) were up-regulated after activation, while those of ST3GalIV and beta1-->4GalT-I were expressed constitutively. However, the up-regulation of C2GnT was more dramatic than that of FucT-VII. These results suggest that sLe(X) expression level is regulated by C2GnT during tonsillar B cell activation.
Nakamura
M
M
Division of Molecular Hemopoiesis, Center for Molecular Medicine, Jichi Medical School, Minamikawachi, Tochigi, Japan. owlmnaka@jichi.ac.jp
Ishida
T
T
Kikuchi
J
J
Furukawa
Y
Y
Matsuda
M
M
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis* / Q000235:genetics; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis* / Q000235:genetics; D014066:Palatine Tonsil / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D016256:Lewis X Antigen; D008934:Mitogens; D005647:Fucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; C031692:galactoside 3-fucosyltransferase