Oxygen depletion during in vitro photodynamic therapy: structure-activity relationships of sulfonated aluminum phthalocyanines.
Photodynamically induced oxygen depletion has been measured in an Ehrlich ascites mouse tumor cell line using a Clark-type electrode. Cells are loaded with aluminum phthalocyanines, sulfonated to different degrees (A1PcS(n), n = 0,2,3,4) and consisting of various isomeric species. Different cell lines and incubation procedures are used in order to investigate the cellular uptake mechanism. Uptake (in units of molecules/cell), post-irradiation redistribution and A1PcS(n) photodegradation are measured using spectroscopic techniques. For a given sensitizer, the oxygen depletion rate per cell increases sublinearly with uptake and superlinearly with cell density. In order to compare oxygen depletion rates of different compounds, we have defined the biological quantum yield (BQY) as the number of oxygen molecules that disappear per absorbed photon. The BQY is independent of uptake and cell density; therefore, it denotes the intrinsic photoactivity of a sensitizer. Sensitizers with high BQY show efficient post-irradiation intracellular redistribution. Photodegradation during irradiation is similar for all sensitizers (20-30%).
Edrei
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Department of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
Kimel
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D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D007211:Indoles / Q000494:pharmacology*; D007834:Lasers; D008027:Light; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology*; D010100:Oxygen / Q000032:analysis; D010778:Photochemotherapy; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D007211:Indoles; D009942:Organometallic Compounds; C060570:aluminum phthalocyanine; D000535:Aluminum; D010100:Oxygen