On-line immunoaffinity extraction and HPLC analysis of flunitrazepam and its main metabolites in serum.
A sensitive, simple, and rapid method for the determination of flunitrazepam and its major metabolites (7-aminoflunitrazepam, 7-acetamidoflunitrazepam, and norflunitrazepam) in serum and plasma is presented. The on-line procedure uses an immobilized, highly reusable antibody against benzodiazepines for selective extraction from serum followed by analysis by high-performance liquid chromatography with ultraviolet detection. This reliable method provides a limit of detection of 1 ng/mL serum, and results are obtained in less than 40 min.
Deinl
I
I
Institute of Forensic Medicine, Erlangen, Germany.
Franzelius
C
C
Angermaier
L
L
Mahr
G
G
Machbert
G
G
D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005445:Flunitrazepam / Q000031:analogs & derivatives / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005445:Flunitrazepam