Tn5: A molecular window on transposition.
DNA transposition is an underlying process involved in the remodeling of genomes in all types of organisms. We analyze the multiple steps in cut-and-paste transposition using the bacterial transposon Tn5 as a model. This system is particularly illuminating because of the existence of structural, genetic, and biochemical information regarding the two participating specific macromolecules: the transposase and the 19-bp sequences that define the ends of the transposon. However, most of the insights should be of general interest because of similarities to other transposition-like systems such as HIV-1 DNA integration into the host genome.
Reznikoff
W S
WS
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin, 53706, USA. reznikoff@biochem.wisc.edu
Bhasin
A
A
Davies
D R
DR
Goryshin
I Y
IY
Mahnke
L A
LA
Naumann
T
T
Rayment
I
I
Steiniger-White
M
M
Twining
S S
SS
D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D011485:Protein Binding; D019895:Transposases / Q000737:chemistry / Q000378:metabolism
D004251:DNA Transposable Elements; D004247:DNA; D019895:Transposases