title: Comparison of detection of extended-spectrum beta-lactamases by agar dilution method, E-test ESBL screen and double disk test.
abstract: The extended-spectrum beta-lactamases (ESBL) are derived from TEM-or SHV-enzymes. They mediate resistance to broad-spectrum beta-lactams and can cause infectious outbreaks in hospitals. Rapid recognition and diagnosis are important for the clinician to prescribe more effective treatment. In the present study, a group of 52 probable ESBL-producing Klebsiella pneumoniae and Escherichia coli having a suspected resistant antibiogram phenotype were included. The E-test ESBL screen and the double disk test were performed for these isolates for detection of ESBL-producing strains, as compared with the conventional agar dilution method. The agreement between the E-test ESBL screen or the double disk test and the conventional agar dilution method was good and the degree of agreement were 86.5% and 92.3% respectively. The results showed that both the E-test ESBL screen and the double disk test were useful and convenient for detection of ESBLs.
authors.affiliation: Section of Infectious Diseases, Veterans General Hospital, Taichung, Taiwan, ROC.
authors.forename: C Y
authors.initials: CY
authors.lastname: Tseng
authors.forename: P Y
authors.initials: PY
authors.lastname: Liu
authors.forename: W L
authors.initials: WL
authors.lastname: Wu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lau
authors.forename: B S
authors.initials: BS
authors.lastname: Hu
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Shi
authors.forename: Y H
authors.initials: YH
authors.lastname: Lin
mesh_terms: D000362:Agar; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000362:Agar; D001618:beta-Lactamases