Recombinant expression of late genes agno-2a and agno-2b of avian polyomavirus BFDV.
Budgerigar fledgling disease virus (BFDV) genome contains two times two (two pairs) open reading frames (agnogenes) at the 5' end of the late coding region. Recombinant influenza A viruses were constructed to express the second pair of BFDV agnoproteins, agno-2a and agno-2b, with a fusion of a histidine-tag at their carboxy-termini, respectively. Specific proteins were detected in Western blot analysis using anti histidine-tag monoclonal antibody. By indirect immunofluorescence experiments agno-2a and agno-2b were shown to be located on the surface and in the perinuclear and cytoplasmic areas of infected cells. Comparisons of the expression patterns of BFDV agno-2a and agno-2b with that of simian virus 40 agnoprotein reveal high similarity, suggesting that they might have the same function(s) in polyomavirus infectious cycle.
Liu
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Institute of Microbiology and Molecular Biology, University of Giessen, Germany. liuq@sask.uasak.ca
Hobom
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D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds / Q000821:virology*; D015153:Blotting, Western; D019084:Fluorescent Antibody Technique, Indirect; D006639:Histidine / Q000235:genetics; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013539:Simian virus 40 / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins; D000086082:Viroporin Proteins
D006639:Histidine; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins