Dynamin 2 is required for phagocytosis in macrophages.
Cells internalize soluble ligands through endocytosis and large particles through actin-based phagocytosis. The dynamin family of GTPases mediates the scission of endocytic vesicles from the plasma membrane. We report here that dynamin 2, a ubiquitously expressed dynamin isoform, has a role in phagocytosis in macrophages. Dynamin 2 is enriched on early phagosomes, and expression of a dominant-negative mutant of dynamin 2 significantly inhibits particle internalization at the stage of membrane extension around the particle. This arrest in phagocytosis resembles that seen with inhibitors of phosphoinositide 3-kinase (PI3K), and inhibition of PI3K prevents the recruitment of dynamin to the site of particle binding. Although expression of mutant dynamin in macrophages inhibited particle internalization, it had no effect on the production of inflammatory mediators elicited by particle binding.
Gold
E S
ES
Department of Immunology, University of Washington, Seattle, Washington 98195, USA.
Underhill
D M
DM
Morrissette
N S
NS
Guo
J
J
McNiven
M A
MA
Aderem
A
A
D000818:Animals; D034284:Dynamin I; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000502:physiology*; D007249:Inflammation; D017737:Macrophages, Peritoneal / Q000502:physiology*; D051379:Mice; D008870:Microtubules / Q000502:physiology; D010587:Phagocytosis / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology
D019869:Phosphatidylinositol 3-Kinases; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D034281:Dynamins