title: TGF-beta1 and IGF-1 expression are differently regulated by serum in metastatic and non-metastatic human breast cancer cells.
abstract: Transforming growth factor-beta (TGF-beta) exerts an inhibitory effect on epithelial cell proliferation while insulin-like growth factor-1 (IGF-1) is a positive regulator of proliferation and together they may participate in driving neoplastic progression. The regulation of TGF-beta1 and IGF-1 gene expression was analyzed in an in vitro model of an estrogen receptor positive (ER+), non-metastatic (MCF-7) and an (ER-), metastatic (MDA-MB-435) breast cancer cell line, respectively. Our results indicate a loss of the regulation of TGF-beta1 and the gain of the expression and upregulation of IGF-1 pathways during malignant progression. These data demonstrate that two factors, convergent on cell growth, can have divergent roles in the regulation of the expression of TGF-beta1.
authors.affiliation: Centro di Studio sui Mitocondri e Metabolismo Energetico, C.N.R., Bari, I-70126 Bari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Perlino
authors.forename: S
authors.initials: S
authors.lastname: Tommasi
authors.forename: L
authors.initials: L
authors.lastname: Moro
authors.forename: A
authors.initials: A
authors.lastname: Bellizzi
authors.forename: E
authors.initials: E
authors.lastname: Marra
authors.forename: V
authors.initials: V
authors.lastname: Casavola
authors.forename: S J
authors.initials: SJ
authors.lastname: Reshkin
mesh_terms: D001769:Blood; D001943:Breast Neoplasms; D002455:Cell Division / Q000502:physiology; D018450:Disease Progression; D004958:Estradiol / Q000502:physiology; D005246:Feedback; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics*; D009362:Neoplasm Metastasis; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D016212:Transforming Growth Factor beta; D004958:Estradiol; D007334:Insulin-Like Growth Factor I