Conditional site-specific integration into human chromosome 19 by using a ligand-dependent chimeric adeno-associated virus/Rep protein.
It is of great interest for gene therapy to develop vectors that drive the insertion of a therapeutic gene into a chosen specific site on the cellular genome. Adeno-associated virus (AAV) is unique among mammalian viruses in that it integrates into a distinct region of human chromosome 19 (integration site AAVS1). The inverted terminal repeats (ITRs) flanking the AAV genome and the AAV-encoded nonstructural proteins Rep78 and/or Rep68 are the only viral elements necessary and sufficient for site-specific integration. However, it is also known that unrestrained Rep activity may cause nonspecific genomic rearrangements at AAVS1 and/or have detrimental effects on cell physiology. In this paper we describe the generation of a ligand-dependent form of Rep, obtained by fusing a C-terminally deleted Rep68 with a truncated form of the hormone binding domain of the human progesterone receptor, which does not bind progesterone but binds only its synthetic antagonist RU486. The activity of this chimeric protein, named Rep1-491/P, is highly dependent on RU486 in various assays: in particular, it triggers site-specific integration at AAVS1 of an ITR-flanked cassette in a ligand-dependent manner, as efficiently as wild-type Rep68 but without generating unwanted genomic rearrangement at AAVS1.
Rinaudo
D
D
Department of Genetics, Istituto di Ricerche di Biologia Molecolare, I.R.B.M.-Piero Angeletti, 00040 Pomezia (Rome), Italy.
Lamartina
S
S
Roscilli
G
G
Ciliberto
G
G
Toniatti
C
C
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002888:Chromosomes, Human, Pair 19; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D015735:Mifepristone / Q000494:pharmacology; D011980:Receptors, Progesterone / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D016662:Virus Integration
D004268:DNA-Binding Proteins; D008024:Ligands; D011980:Receptors, Progesterone; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D015735:Mifepristone; D004265:DNA Helicases