Formation of L-malic acid by yeasts of the genus Dipodascus.
The yeast strains of the genus Dipodascus were used for the bioconversion of fumaric acid to L-malic acid. Under nongrowth conditions, the fumarase activity in the intact cells or in the cell-free extract of Dipodascus was 10 times higher than that of Saccharomyces cerevisiae cells. Pretreatment of the Dipodascus with malonate was not necessary because succinate was not detected as a by-product. The fumarase activity in Dipodascus magnusii CCM 8235 was increased approximately 100% when Triton X-305 (0.1%) was added to the reaction mixture.
Rosenberg
M
M
Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Technical University, Bratislava, Slovakia. miko@chelin.chtf.stuba.sk
Miková
H
H
Kristofíková
L
L
D018533:Biomass; D003470:Culture Media; D003902:Detergents / Q000494:pharmacology; D005649:Fumarate Hydratase / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D004718:Saccharomycetales / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D003902:Detergents; D008293:Malates; C030298:malic acid; D005649:Fumarate Hydratase