title: Fine physical and transcript mapping of a 1.8 Mb region spanning the locus for childhood acute lymphoblastic leukemia on chromosome 12p12. 3.
abstract: Rearrangements of the short arm of chromosome 12 are frequently observed in hematological disorders. Previous studies of loss of heterozygosity identified a small genetic interval on chromosome 12p12.3 that is frequently deleted in childhood acute lymphoblastic leukemia (ALL). Two genes, ETV6/TEL and p27/KIP1, are located in this interval. Evidence has accumulated that an as-yet unidentified tumor suppressor gene is closely linked to these. To facilitate the identification of candidate genes, a long-range high-resolution restriction map of the ALL locus was constructed using a contig of YAC clones. Several marker loci, including 11 STS, three newly developed YAC end-based STS, six EST, and seven genes were unambiguously positioned in the new map. The map covers 1.8Mb and extends from the distal salivary proline-rich protein gene cluster to the proximal p27/KIP1 gene. The data confirmed the order tel-D12S358-p27/KIP1-cen and excluded p27/KIP1 as a candidate tumor suppressor gene. The critical region delimited by D12S89 and D12S358 is a 750kb CpG-island rich region that includes the 240kb TEL/ETV6 gene as well as CLAPS3 (clathrin-adaptor small chain 3). The new map provides a molecular framework for the identification of novel genes and transcriptional units in the ALL interval.
authors.affiliation: Division of Hematology-Oncology, Charles-Bruneau Cancer Center, Research Center, Sainte-Justine Hospital, 3175 Côte Ste-Catherine, Montreal, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Aïssani
authors.forename: D
authors.initials: D
authors.lastname: Sinnett
mesh_terms: D033963:Adaptor Protein Complex 3; D034002:Adaptor Protein Complex sigma Subunits; D000818:Animals; D018797:Cell Cycle Proteins; D002648:Child; D018244:Chromosomes, Artificial, Yeast; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D020451:Contig Mapping; D006224:Cricetinae; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D020224:Expressed Sequence Tags; D006801:Humans; D006822:Hybrid Cells; D018895:Microsatellite Repeats; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D010455:Peptides / Q000235:genetics; D020161:Physical Chromosome Mapping; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D055232:Proline-Rich Protein Domains; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D016324:Sequence Tagged Sites; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D033963:Adaptor Protein Complex 3; D034002:Adaptor Protein Complex sigma Subunits; D018797:Cell Cycle Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004247:DNA; D004268:DNA-Binding Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D000097764:ETS Translocation Variant 6 Protein; C115637:AP3S1 protein, human