title: Atlantic salmon (Salmo salar L.) skin contains a novel kininogen and another cysteine proteinase inhibitor.
abstract: We describe the purification and characterization of two novel cysteine proteinase inhibitors found in Atlantic salmon skin. One of these, salmon kininogen, has a molecular mass of 52 kDa as determined by matrix-assisted laser desorption/ionization time-of-flight MS, is multiply charged with pI values of 4.0, 4.2 and 4.6 and shows homology to kininogens including the bradykinin motif. The other, salarin, has a molecular weight of 43 kDa, a pI of 5.1 and shows weak homology to cysteine proteinases. Both proteins are N- and O-glycosylated and inhibit papain and ficin but not trypsin.
authors.affiliation: Institute of Biotechnology, Protein Chemistry Laboratory, University ofHelsinki, Finland. aylonen@operoni.helsinki.fi
authors.forename: A
authors.initials: A
authors.lastname: Ylönen
authors.forename: A
authors.initials: A
authors.lastname: Rinne
authors.forename: J
authors.initials: J
authors.lastname: Herttuainen
authors.forename: J
authors.initials: J
authors.lastname: Bogwald
authors.forename: M
authors.initials: M
authors.lastname: Järvinen
authors.forename: N
authors.initials: N
authors.lastname: Kalkkinen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D029941:Fish Proteins; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006031:Glycosylation; D007526:Isoelectric Point; D007704:Kininogens / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020449:Repetitive Sequences, Amino Acid; D020317:Salmo salar / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015853:Cysteine Proteinase Inhibitors; D029941:Fish Proteins; D006023:Glycoproteins; D007704:Kininogens; C403188:salarin protein, Salmo salar