Improved detection of five closely related ruminant alphaherpesviruses by specific amplification of viral genomic sequences.
The detection and discrimination of five closely related ruminant alphaherpesviruses, bovine herpesvirus 1 (BHV-1), bovine herpesvirus 5 (BHV-5), caprine herpesvirus 1 (CapHV-1), cervine herpesvirus 1 (CerHV-1), and rangiferine herpesvirus 1 (RanHV-1), were achieved by the development of specific PCR systems. The highly variable N-terminal of the glycoprotein C was chosen to select the diagnostic primers, except for the CerHV-1 primers, which targeted the glycoprotein D region. All the assays proved specific since no heterologous virus was amplified. BHV-1 and BHV-5 were detected by using the same PCR assay and the different sizes of the amplification products allowed their identification on agarose gels. The practical diagnostic applicability of the novel PCR assays, with special regard to the BHV-1 system, has been evaluated on clinical samples from experimentally infected animals.
Ros
C
C
Department of Virology, The National Veterinary Institute, Biomedical Center, Uppsala, Sweden. carlos.ros@bmc.uu.se
Riquelme
M E
ME
Forslund
K O
KO
Belák
S
S
D018138:Alphaherpesvirinae / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D003670:Deer / Q000821:virology; D005784:Gene Amplification; D016679:Genome, Viral; D006041:Goats / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012053:Reindeer / Q000821:virology; D012418:Ruminants / Q000821:virology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
D017931:DNA Primers; D004279:DNA, Viral