A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines.
In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1alpha (IL1alpha) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler.
Spagnuolo-Weaver
M
M
Department of Virology, Veterinary Sciences Division, Stormont, Belfast, UK. m.spagnuolo@qub.a.cuk
Fuerst
R
R
Campbell
S T
ST
Meehan
B M
BM
McNeilly
F
F
Adair
B
B
Allan
G
G
D000199:Actins / Q000235:genetics; D000818:Animals; D016207:Cytokines / Q000032:analysis* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005069:Evaluation Studies as Topic; D005470:Fluorometry / Q000379:methods*; D015870:Gene Expression; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics; D007376:Interleukin-2 / Q000032:analysis / Q000235:genetics; D007962:Leukocytes / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013552:Swine
D000199:Actins; D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary; D007375:Interleukin-1; D007376:Interleukin-2; D012333:RNA, Messenger