title: Properties of endothelium and smooth muscle cells in canine femoral arteries after lumbar sympathectomy.
abstract: OBJECTIVE
  To find out whether the lumbar sympathectomy modulated the endothelial function (as measured by nitric oxide (NO) and prostacyclin (PGI2), and blood flow in the canine femoral artery.
  
  
  METHODS
  Laboratory experiments.
  
  
  METHODS
  Teaching hospital, Japan.
  
  
  METHODS
  16 mongrel dogs.
  
  
  METHODS
  Unilateral sympathectomy from L3 to L6.
  
  
  METHODS
  Five weeks later, the changes in blood flow, the endothelium-dependent responses and the PGI2 production in the canine femoral arteries were measured.
  
  
  RESULTS
  The median (range) blood flow of left (denervated) and right (innervated) femoral arteries was 162 ml/min (122-330) and 65 ml/min (40-92), respectively. There was a significant difference between the two groups (p < 0.01). The endothelium-dependent relaxations to acetylcholine, adenosine diphosphate (ADP) and A23187 were comparable. The amounts of PGI2 produced in the two groups were similar. Direct relaxation in response to sodium nitroprusside was also similar in the two groups.
  
  
  CONCLUSIONS
  Lumbar sympathectomy did not alter the endothelial function, although the median blood flow in the denervated femoral arteries was significantly higher than in the innervated ones. The continuous vasodilatation after sympathectomy may be a more potent factor in the regulation of vascular tonus than the physiological regulation of NO and PGI2.
authors.affiliation: Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Komori
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Ohta
authors.forename: S
authors.initials: S
authors.lastname: Funahashi
authors.forename: M
authors.initials: M
authors.lastname: Ishida
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Onohara
authors.forename: M
authors.initials: M
authors.lastname: Kume
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000818:Animals; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D011464:Epoprostenol / Q000378:metabolism; D005263:Femoral Artery / Q000166:cytology* / Q000502:physiology; D008160:Lumbosacral Plexus / Q000601:surgery*; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology*; D009569:Nitric Oxide / Q000378:metabolism; D012039:Regional Blood Flow; D013562:Sympathectomy; D014664:Vasodilation / Q000502:physiology
chemical_list: D009569:Nitric Oxide; D011464:Epoprostenol