title: Deletion of chromosome 1p and loss of expression of alkaline phosphatase indicate progression of meningiomas.
abstract: Meningiomas are cytogenetically characterized by loss of one chromosome 22 as a typical primary aberration and progression-associated secondary chromosome changes, of which monosomy 1p is the most common. The aim of this study was to evaluate the significance of monosomy 1p and enzyme activity loss of tissue nonspecific alkaline phosphatase (ALPL), whose gene maps to chromosome 1p36.1-p34, as parameters for the diagnosis of progression-prone meningiomas. We analyzed smear preparations of 56 meningiomas and additional paraffin sections of 17 of the cases by two-color fluorescence in situ hybridization (FISH) using the D1Z1 and D1Z2 probes and by a metaphase cytogenetic analysis of 30 of these tumors. The results were compared to clinical and morphological parameters and the expression of ALPL. Smear preparations showed deletion of 1p36 in 27% of common-type, 70% of atypical (intermediate-type), and 100% of anaplastic meningiomas. Monosomy 1p, as detected by FISH or the karyotype, was strongly associated with complete loss of ALPL activity. Intermediate-type and anaplastic meningiomas of younger patients displayed an increasing rate of cells with trisomy 1q and relative loss of 1p. The highly significant correlation of FISH results and ALPL histochemistry with clinical parameters gives evidence of their strong prognostic relevance. The complete activity loss of ALPL and the immunologically detected loss of ALPL protein in areas of meningiomas with monosomy 1p indicate a cytogenetically undetectable inactivation of the homologous Alpl allele. The apparently homozygous loss of expression of ALPL supports the notion that Alpl is a candidate tumor suppressor gene in meningiomas.
authors.affiliation: Department of Human Genetics, Medical School of the University of the Saarland, Homburg/Saar, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Müller
authors.forename: W
authors.initials: W
authors.lastname: Henn
authors.forename: I
authors.initials: I
authors.lastname: Niedermayer
authors.forename: R
authors.initials: R
authors.lastname: Ketter
authors.forename: W
authors.initials: W
authors.lastname: Feiden
authors.forename: W I
authors.initials: WI
authors.lastname: Steudel
authors.forename: K D
authors.initials: KD
authors.lastname: Zang
authors.forename: H
authors.initials: H
authors.lastname: Steilen-Gimbel
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics*; D001706:Biopsy; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D018450:Disease Progression; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008577:Meningeal Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D009006:Monosomy; D014314:Trisomy
chemical_list: D000469:Alkaline Phosphatase