Chicken embryonic stem cells and transgenic strategies.
The production of transgenic birds is an important goal for both fundamental and applied biology. Different methods have been employed to generate transgenic chickens, including microinjection, use of retroviruses and transfection of primordial germ or embryonic germ cells. In this review we will briefly describe these techniques and our efforts to obtain genetically modified avian embryonic stem (ES) cells using liposomes. This latter technique should allow us to modify chicken ES cells with a high efficiency, permitting the rapid generation of transgenic bird lines.
Pain
B
B
Laboratoire de Biologie, CNRS UMR 49-LA INRA 913, Ecole Normale Supérieure de Lyon, France. bertrandpain@vivalis.com
Chenevier
P
P
Samarut
J
J
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002642:Chick Embryo; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*