title: The Legionella pneumophila prp locus; required during infection of macrophages and amoebae.
abstract: Transposon mutagenesis was performed using mTn 10phoA to identify Legionella pneumophila genes that are expressed under certain in vitro conditions, and are required for intracellular replication. Of the 1653 PhoA fusions examined, 19 PhoA(+)fusion mutants were isolated and screened for differential expression of fusion proteins after growth at 30 or 37 degrees C, in the presence of low iron, or increased magnesium concentrations. The mutants were examined for their cytopathogenicity and intracellular replication within U937 macrophage-like cells and the protozoan Hartmannella vermiformis. One of the mutants generated, BS10, was defective in its multiplication within U937 macrophage-like cells and H. vermiformis. The defect in BS10 was complemented with a cosmid clone containing the wild type locus. The open reading frame interrupted by the insertion was homologous to prpD of Salmonella typhimurium and mmgE of Bacillus subtilis.
authors.affiliation: Department of Microbiology and Immunology, University of Kentucky Chandler Medical Center, Lexington, Kentucky, 40536-0084, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Stone
authors.forename: A
authors.initials: A
authors.lastname: Brier
authors.forename: Y A
authors.initials: YA
authors.lastname: Kwaik
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D002460:Cell Line; D015169:Colony Count, Microbial; D003470:Culture Media; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D005816:Genetic Complementation Test; D006249:Hartmannella / Q000382:microbiology*; D006801:Humans; D007501:Iron / Q000378:metabolism; D016952:Legionella pneumophila / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D008264:Macrophages / Q000382:microbiology*; D008274:Magnesium / Q000378:metabolism; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D013696:Temperature; D014774:Virulence / Q000235:genetics
chemical_list: D003470:Culture Media; D004251:DNA Transposable Elements; D007501:Iron; D018844:Cyclin-Dependent Kinases; D000469:Alkaline Phosphatase; D008274:Magnesium