Antigenic properties of LPS extracted from Bacteroides fragilis enterotoxin producing strains.
Antigenic properties of enterotoxigenic Bacteroides fragilis (ETBF) strains isolated in Poland were compared with reference strains. The agglutination and passive hemagglutination, SDS-PAGE analysis and immunoblotting tests as well as analyses of sugars and fatty acids were performed with lipopolysaccharide (LPS) preparations obtained from water-phase of phenol-water extracts. Some differences in serological reactivity between ETBF antigens were observed. The antigen of the NTBF (nonenterotoxigenic) reference strain IPL E-323 expressed weak cross-reactivity with sera against whole cells of ETBF strains in serological tests. There were some differences observed between ETBF and NTBF strains in fatty acids and sugar composition. The LPS preparations probably possess a common core structure and the O-specific polysaccharides of variable chain length.
Meisel-Mikołajczyk
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F
Department of Medical Microbiology, Medical Academy, Warsaw, Poland. fmikolaj@ib.amwaw.edu.pl
Kot
K
K
Kłos
W
W
Rouyan
G S
GS
Mieszała
M
M
Gamian
A
A
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D001441:Bacteroides fragilis / Q000737:chemistry / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D019073:HT29 Cells; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D013552:Swine
D000942:Antigens, Bacterial; D002241:Carbohydrates; D004768:Enterotoxins; D005227:Fatty Acids; D008070:Lipopolysaccharides