Inhibition of glucose uptake and suppression of glucose transporter 1 mRNA expression in L929 cells by tumour necrosis factor-alpha.
Recombinant human tumour necrosis factor-alpha (rhTNF-alpha) arrested the growth and suppressed glucose uptake of mouse fibrosarcoma L929 cells in vitro. When the cells were treated with rhTNF-alpha for 24 hours, the mRNA level of glucose transporter 1 (GLUT 1), which is the only GLUT found to be present in L929 cells in our study, was suppressed in a dose-dependent manner. Since the growth of tumour cells depends mainly on glucose catabolism, our findings may indicate that rhTNF-alpha inhibits L929 cells growth by lowering the glucose transport through suppression of GLUT 1 mRNA expression in the cells.
Liong
E
E
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT.
Kong
S K
SK
Au
K K
KK
Li
J Y
JY
Xu
G Y
GY
Lee
Y L
YL
Kwok
T T
TT
Choy
Y M
YM
Lee
C Y
CY
Fung
K P
KP
D000818:Animals; D015152:Blotting, Northern; D002470:Cell Survival / Q000187:drug effects; D003847:Deoxyglucose / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D005354:Fibrosarcoma / Q000378:metabolism* / Q000473:pathology; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics*; D051272:Glucose Transporter Type 1; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D051272:Glucose Transporter Type 1; D006131:Growth Inhibitors; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C495677:SLC2A1 protein, human; C495678:Slc2a1 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D003847:Deoxyglucose; D005947:Glucose