A phage antibody identifying an 80-kDa membrane glycoprotein exclusively expressed on a subpopulation of activated B cells and hairy cell leukemia B cells.
We have isolated a phage display library-derived monoclonal antibody, phab V-3, that identifies a membrane glycoprotein of approximately 80 kDa which is expressed on a subpopulation of activated B lymphocytes in secondary lymphoid organs. In agreement with their activated phenotype, phab V-3(+) B cells display a blast-like morphology, and are prone to spontaneous apoptosis in vitro, unless rescued by stimulation with CD40 ligand (CD40L). The expression of the phab V-3 molecule coincides with B cells that produce high levels of IgM, IgG and IgA in vitro upon stimulation with CD40L in combination with IL-2 and IL-10. Immunofluorescent analysis of B cell malignancies unveiled that the phab V-3 molecule was uniquely expressed on hairy cell leukemia (HCL) B cells. Similar to phab V-3(+) tonsils B cells, HCL B cells have been reported to express CD11c, CD95 and CD27, which might indicate that the phab V-3(+) B cells in HCL are the malignant counterpart of the phab V-3(+) B cell subpopulation.
van Der Vuurst De Vries
A R
AR
Department of Immunology University Medical Center Utrecht, Utrecht, The Netherlands.
Logtenberg
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D001435:Bacteriophages; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D019151:Peptide Library; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D008562:Membrane Glycoproteins; D019151:Peptide Library