Incorporation of adeno-associated virus in a calcium phosphate coprecipitate improves gene transfer to airway epithelia in vitro and in vivo.
Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaP(i) coprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.
Walters
R W
RW
Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
Duan
D
D
Engelhardt
J F
JF
Welsh
M J
MJ
D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry*; D011232:Chemical Precipitation; D000229:Dependovirus / Q000737:chemistry / Q000235:genetics*; D004848:Epithelium / Q000378:metabolism; D018014:Gene Transfer Techniques / Q000592:standards*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic; D014132:Trachea / Q000378:metabolism*
D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous