A procedure for the preparation of GM3 ganglioside from GM1-lactone.
A simple procedure is described for preparing GM3 ganglioside, from a few milligrams to grams, from GM1-lactone (Sonnino et al., (1985) Glycoconjugate J 2: 343-54) [1]. The synthesis was carried out under the following optimal conditions: 30 mM GM1-lactone in 0.25 M H2SO4 in DMSO, 30 min, 70 degrees C, nitrogen atmosphere, strong stirring. The yield of GM3 was 55%. The procedure applied to milligram amounts of GD1b-dilactone gave GD3 ganglioside.
Mauri
L
L
Study Center for the Functional Biochemistry of Brain Lipids, Department of Medical Chemistry and Biochemistry - LITA-Segrate, The Medical School, University of Milan, Milano, Italy.
Casellato
R
R
Kirschner
G
G
Sonnino
S
S
D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000737:chemistry; D005679:G(M3) Ganglioside / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy
D005679:G(M3) Ganglioside; C060696:ganglioside M1 lactone; D005677:G(M1) Ganglioside