Physical mapping and promoter structure of the murine cAMP-specific phosphodiesterase pde4a gene.
The Pde4a gene is a mammalian homolog of the dunce learning and memory gene of Drosophila melanogaster and encodes cAMP-specific phosphodiesterases, targets for drugs with antidepressant and anti-inflammatory actions in humans. We have analyzed the intron/exon and promoter structure of the murine Pde4a gene. Pde4a encodes at least two different transcripts, each generated by alternative mRNA splicing and the use of alternative promoters. The majority of Pde4a exons are tightly clustered at the 3' end of the gene. The 5' region of the gene contains at least one widely separated exon, which encodes the 5' end of a distinct mRNA transcript and contains a separate promoter and transcriptional start site. Analysis of YAC clones determined that the Pde4a gene maps to the 4-cM region of Chromosome (Chr) 9, close to Ldlr and Epor, in a region syntenic to human PDE4A.
Olsen
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AE
Medical Service, Department of Veterans Affairs Medical Center, and Department of Medicine (Division of Oncology), University of Utah Health Sciences Center, 500 Foothill Blvd, Salt Lake City, Utah 84148, USA.
Bolger
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GB
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018244:Chromosomes, Artificial, Yeast / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry; D005091:Exons / Q000235:genetics; D015723:Gene Library; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D015105:3',5'-Cyclic-AMP Phosphodiesterases