title: Immobilization of cholesterol oxidase on Formvar using organic solvents.
abstract: The present study describes a new immobilization support for the preparation of enzyme membrane in the presence of organic solvents. The support was composed of Formvar solubilization in organic solvents with enzyme. More than 90% of the enzyme was immobilized on the membrane. The membrane was prepared by mixing 4% Formvar in organic solvents and 1% cholesterol oxidase was immobilized by entrapment technique. Practically no leaching of the entrapped enzyme was observed. The resultant immobilized enzyme membrane was stored at 4 degrees C for 10 days without losing its activity. The pH and temperature stabilities were greater than those of the native enzyme. The immobilized enzyme membrane has a long life due to its hydrophobic nature, as compared with other membranes.
authors.affiliation: Centre for Biochemical Technology, Mall Road, Delhi-110007, India.
authors.forename: H
authors.initials: H
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: P
authors.initials: P
authors.lastname: Kumari
authors.forename: S
authors.initials: S
authors.lastname: Jyotirmai
authors.forename: N B
authors.initials: NB
authors.lastname: Tulsani
mesh_terms: D002789:Cholesterol Oxidase / Q000737:chemistry* / Q000378:metabolism; D003124:Colorimetry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D009930:Organic Chemicals; D011145:Polyvinyls; D012997:Solvents; C013215:polyvinyl acetate; D002789:Cholesterol Oxidase