Requirement of functional ethylene-insensitive 2 gene for efficient resistance of Arabidopsis to infection by Botrytis cinerea.
Inoculation of wild-type Arabidopsis plants with the fungus Alternaria brassicicola results in systemic induction of genes encoding a plant defensin (PDF1.2), a basic chitinase (PR-3), and an acidic hevein-like protein (PR-4). Pathogen-induced induction of these three genes is almost completely abolished in the ethylene-insensitive Arabidopsis mutant ein2-1. This indicates that a functional ethylene signal transduction component (EIN2) is required in this response. The ein2-1 mutants were found to be markedly more susceptible than wild-type plants to infection by two different strains of the gray mold fungus Botrytis cinerea. In contrast, no increased fungal colonization of ein2-1 mutants was observed after challenge with avirulent strains of either Peronospora parasitica or A. brassicicola. Our data support the conclusion that ethylene-controlled responses play a role in resistance of Arabidopsis to some but not all types of pathogens.
Thomma
B P
BP
F.A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, K. Mercierlaan 92, B-3001 Heverlee-Leuven, Belgium.
Eggermont
K
K
Tierens
K F
KF
Broekaert
W F
WF
D000085:Acetates / Q000494:pharmacology; D000528:Alternaria / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology*; D029681:Arabidopsis Proteins; D020171:Botrytis / Q000472:pathogenicity*; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000502:physiology; D015398:Signal Transduction
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C119963:EIN2 protein, Arabidopsis; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C072239:methyl jasmonate; C036216:ethylene