Spectral properties of the oxyferrous complex of the heme domain of cytochrome P450 BM-3 (CYP102).
Here we describe for the first time the formation of a complex of reduced CYP102 (cytochrome P450 BM-3) heme domain with molecular oxygen. To stabilize the oxycomplex, the experiments had to be done under argon atmosphere at cryogenic temperatures (-25 degrees C) in the presence of 50% glycerol. The spectral properties of this species were different from those of another P450-type autosuffisant enzyme, i.e., the neuronal nitric oxide synthase. On the contrary, the oxyferrous complex of CYP102 possesses spectral properties similar to those of complexes of microsomal cytochromes P450, e.g., CYP2B4.
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INSERM Unité 128, IFR 24, 1919 Route de Mende, Montpellier Cedex 5, F-34293, France.
Anzenbacher
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Anzenbacherová
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Gorren
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Munro
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Lange
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D000818:Animals; D001128:Argon / Q000378:metabolism; D001189:Aryl Hydrocarbon Hydroxylases; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D006418:Heme / Q000378:metabolism*; D020035:Holoenzymes / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase; D019001:Nitric Oxide Synthase / Q000737:chemistry / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011817:Rabbits; D013057:Spectrum Analysis; D013250:Steroid Hydroxylases / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D001426:Bacterial Proteins; D005296:Ferrous Compounds; D020035:Holoenzymes; D006418:Heme; D001128:Argon; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D013250:Steroid Hydroxylases; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D001189:Aryl Hydrocarbon Hydroxylases; C050356:steroid 15-alpha-hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases; D005990:Glycerol; D010100:Oxygen