title: Reversal in microfilarial density and T cell responses in human lymphatic filariasis.
abstract: This study reports reversals in microfilarial density and the accompanying changes in cellular immune responses to filarial antigens of 39 individuals (11 microfilaria-positives, 22 microfilaria-negatives and six converters) living in an area endemic for brugian filariasis. Microfilarial counts decreased from April, the end of the rainy season to July, middle of the dry season (g.m. 88 mf/ml and 38 mf/ml, respectively; P = 0.001) and subsequently increased in November, the beginning of the rainy season (P = 0.088). Whereas the proliferative responses remained low throughout the study period in microfilaraemic individuals, in amicrofilaraemics these responses changed in the opposite direction to that of microfilarial densities. In three converters, proliferation changed in the opposite direction to the presence or absence of microfilariae. Cytokine analysis in the converters revealed that interferon-gamma was most affected by the shifts in microfilarial densities. In contrast, interleukin-4 responses showed little correlation with changes in parasite densities.
authors.affiliation: Department of Parasitology, Leiden University, Wassenaarseweg 62, Postbus 9605, 2300 RC Leiden, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Sartono
authors.forename: C
authors.initials: C
authors.lastname: Lopriore
authors.forename: Y C
authors.initials: YC
authors.lastname: Kruize
authors.forename: A
authors.initials: A
authors.lastname: Kurniawan-Atmadja
authors.forename: R M
authors.initials: RM
authors.lastname: Maizels
authors.forename: M
authors.initials: M
authors.lastname: Yazdanbakhsh
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D017178:Brugia malayi / Q000254:growth & development*; D016207:Cytokines / Q000378:metabolism; D004605:Elephantiasis, Filarial / Q000276:immunology* / Q000469:parasitology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008842:Microfilariae / Q000254:growth & development* / Q000302:isolation & purification; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016207:Cytokines; D015847:Interleukin-4; D007371:Interferon-gamma