Is Dupuytren's disease caused by an imbalance between proliferation and cell death?
Dupuytren's contracture shares certain properties with malignant tumours, characterized by proliferation and lack of apoptosis, which may be induced by the c-myc oncogene. Because of these similarities, the relationship between the c-myc oncogene expression, bcl-2 oncogene (anti-apoptotic gene) and proliferation was investigated in Dupuytren's disease. Proliferation was assessed by immunohistochemical staining of the mib-1 antibody. Results were compared with those from fibrosarcoma specimens, representing a related malignant tumour. Non-diseased fascia from Dupuytren patients and flexor retinaculum from patients undergoing carpal tunnel release without Dupuytren's disease were used as controls. Expression of c-myc was elevated in primary Dupuytren's disease and fibrosarcoma specimens, whilst recurrent Dupuytren's disease, non-diseased Dupuytren fascia and flexor retinaculum exhibited significantly lower levels. Neither bcl-2 nor mib-1 were detected in Dupuytren's disease, non-diseased fascia or flexor retinaculum, in contrast to fibrosarcoma. The imbalance between proliferation and apoptosis, producing malignant growth was thus confirmed for fibrosarcoma, but not for Dupuytren's disease.
Jemec
B
B
Department of Plastic Surgery, Mount Vernon Hospital, Northwood, Middlesex, UK. bjemec@krota.demon.co.uk
Grobbelaar
A O
AO
Wilson
G D
GD
Smith
P J
PJ
Sanders
R
R
McGrouther
D A
DA
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D016923:Cell Death / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D003593:Cytoplasm / Q000737:chemistry; D003937:Diagnosis, Differential; D004387:Dupuytren Contracture / Q000235:genetics* / Q000473:pathology*; D005205:Fascia / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005354:Fibrosarcoma / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D019254:Genes, bcl-2 / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012008:Recurrence; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric