title: Biocatalytic combinatorial synthesis.
abstract: Combinatorial biocatalysis, based on a principle of the combinatorial use of biosynthetic steps rather than the combinatorial use of reagents, offers a complementary approach to combinatorial chemistry, which, used individually or in connection with synthetic organic transformations, provides access to analogues not readily accessible by chemical synthetic means alone. The issues and strategies particular to this approach are discussed. Examples are given demonstrating these principles as well as the unique advantages of achieving chemo-, regio- and stereoselectivity under mild reaction conditions that biocatalytic methods offer.
authors.affiliation: EnzyMed, Inc., Iowa City, IA 52242, USA. jkrstenansky@enzymed.com
authors.forename: J L
authors.initials: JL
authors.lastname: Krstenansky
authors.forename: Y
authors.initials: Y
authors.lastname: Khmelnitsky
mesh_terms: D000215:Acylation; D001578:Benzopyrans / Q000737:chemistry / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D020860:Subtilisin / Q000737:chemistry / Q000378:metabolism; D014230:Triazoles / Q000737:chemistry / Q000378:metabolism
chemical_list: D001578:Benzopyrans; D004798:Enzymes; D014230:Triazoles; C011852:1-hydroxybenzotriazole; D010088:Oxidoreductases; D042845:Laccase; D008049:Lipase; D020860:Subtilisin; C006741:bergenin