title: Cytochrome p450nor, a novel class of mitochondrial cytochrome P450 involved in nitrate respiration in the fungus Fusarium oxysporum.
abstract: Fusarium oxysporum, an imperfect filamentous fungus performs nitrate respiration under limited oxygen. In the respiratory system, Cytochrome P450nor (P450nor) is thought to catalyze the last step; reduction of nitric oxide to nitrous oxide. We examined its intracellular localization using enzymatic, spectroscopic, and immunological analyses to show that P450nor is found in both the mitochondria and the cytosol. Translational fusions between the putative mitochondrial targeting signal on the amino terminus of P450nor and Escherichia coli beta-galactosidase resulted in significant beta-galactosidase activity in the mitochondrial fraction of nitrate-respiring cells, suggesting that one of the isoforms of P450nor (P450norA) is in anaerobic mitochondrion of F. oxysporum and acts as nitric oxide reductase. Furthermore, these findings suggest the involvement of P450nor in nitrate respiration in mitochondria.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takaya
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Kuwazaki
authors.forename: H
authors.initials: H
authors.lastname: Shoun
authors.forename: F
authors.initials: F
authors.lastname: Maruo
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Takeo
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001692:Biological Transport; D019069:Cell Respiration; D018387:Codon, Initiator / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D005670:Fusarium / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism* / Q000648:ultrastructure; D007527:Isoenzymes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D009566:Nitrates / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D021382:Protein Sorting Signals / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D018387:Codon, Initiator; D007527:Isoenzymes; D009566:Nitrates; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D009569:Nitric Oxide; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; C080610:nitric-oxide reductase (P450); C034747:nitric-oxide reductase; D009609:Nitrous Oxide; D010100:Oxygen