[Discovery of internal sources of the neural retinal regeneration after its detachment in Pleurodeles. II. The radioautography study].
We continued to study internal sources of neural retina regeneration in adult lower vertebrates. A radioautographic study carried out was aimed at the visualization of proliferating cells in different areas of the retina and at different times after its detachment in the newts Pleurodeles waltl. Analysis of serial standards and semithin sections has shown several types of cells capable of incorporating a labeled DNA synthesis precursor. The marker of proliferating cells was incorporated in the cells of Mullerian glia, pigment epithelium, and growth zone and in small neural cells of the vitreal part of the inner nuclear layer. A source of formation of neuroepithelial "insertions" responsible for restoration of the complete cell composition of the retina damaged as a result of detachment should be searched among these cell types. Neither bipolar cells with Landoldt's club in the inner nuclear layer, nor bipolar-like cells in the outer nuclear layer, which are a source of formation of additional photoreceptors, were found among the 3H-thymidine- labeled cells. A conclusion was drawn that the bipolar-like cells of P. waltl are postmitotic cells, that did not reach terminal differentiation.
Grigorian
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Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Poplinskaia
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VA
D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation; D010999:Pleurodeles / Q000502:physiology*; D012038:Regeneration; D012160:Retina / Q000473:pathology / Q000502:physiology*; D012163:Retinal Detachment / Q000473:pathology*
Obnaruzhenie vnutrennikh istochnikov regeneratsii neĭtral'noĭ setchatki posle ee otsloĭki u tritonov. II. Radioavtograficheskoe issledovanie.