[Comparison of immunoassay kits for detection of staphylococcal enterotoxins produced by Staphylococcus aureus].
Four commercial kits, SET-RPLA, RIDASCREEN, SET-EIA, and TECRA, were compared for the efficiency of detecting staphylococcal enterotoxins (SE). There was no non-specific reaction for detection of SE produced by 21 Staphylococcus aureus strains from 5 outbreaks of food poisoning using SET-RPLA, SET-EIA and TECRA kits. The background of the results of RIDASCREEN kit was high and non-specific reactions were present in some strains.


CONCLUSIONS
(i) TECRA kit is suggested to be used for screening SE producing strains; (ii) SET-RPLA and RIDASCREEN kits are suitable for epidemiological investigation of SE types, but the lack of ability for detecting SEE, long time required for testing with SET-RPLA kit and high background when using RIDASCREEN kit must be overcome; and (iii) because of the complicated test procedures and the lack of ability for detecting SEE, the practicality of SET-EIA kit in screening and epidemiological research purposes is low.
Pan
T M
TM
National Institute of Preventive Medicine, Department of Health, Taipei, Taiwan, R.O.C.
Yu
Y L
YL
Chiu
S I
SI
Lin
S S
SS
D004768:Enterotoxins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013202:Staphylococcal Food Poisoning / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000472:pathogenicity*
D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic