title: Effect of mutations within the cys-rich region of potyvirus helper component-proteinase on self-interaction.
abstract: The first approximately 60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment.
authors.affiliation: Institut Jacques Monod, 2 Place Jussieu - Tour 43, 75251 Paris Cedex 05, France1.
authors.forename: Silvio
authors.initials: S
authors.lastname: Urcuqui-Inchima
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, UNICAMP, CP 6109, Campinas - SP, Brazil2.
authors.forename: Ivan G
authors.initials: IG
authors.lastname: Maia
authors.affiliation: Institut Jacques Monod, 2 Place Jussieu - Tour 43, 75251 Paris Cedex 05, France1.
authors.forename: Gabrièle
authors.initials: G
authors.lastname: Drugeon
authors.affiliation: Institut Jacques Monod, 2 Place Jussieu - Tour 43, 75251 Paris Cedex 05, France1.
authors.forename: Anne-Lise
authors.initials: AL
authors.lastname: Haenni
authors.affiliation: Institut Jacques Monod, 2 Place Jussieu - Tour 43, 75251 Paris Cedex 05, France1.
authors.forename: Françoise
authors.initials: F
authors.lastname: Bernardi
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine; D019281:Dimerization; D010450:Endopeptidases / Q000737:chemistry*; D008969:Molecular Sequence Data; D009154:Mutation; D017800:Potyvirus / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D014764:Viral Proteins; D010450:Endopeptidases; D003545:Cysteine