Determination of opiates and cocaine and its metabolites in biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry.
A rapid, sensitive, and specific method for the determination of opiates and cocaine and metabolites in urine, plasma, and blood was established. A one-step extraction followed by liquid chromatography-electrospray ionization tandem mass spectrometry operating in multiple reaction monitoring mode was used. Two chromatographic runs were performed, each in less than 6 min. The lower limit for accurate quantitative determination was 5 microg/L for cocaine and metabolites and 10 microg/L for opiates. Linearity was obtained from 10 to 1000 microg/L. Intraday (n = 6) and interday (n = 6) precisions and recoveries (n = 6) were determined at 10 or 25, 100, and 1000 microg/L concentrations. Precisions with a coefficient of variation less than 15% were obtained. Recoveries between 85 and 115% were determined.
Cailleux
A
A
Laboratoire de Pharmacologie-Toxicologie, CHU, Angers, France.
Le Bouil
A
A
Auger
B
B
Bonsergent
G
G
Turcant
A
A
Allain
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D009294:Narcotics / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods
D009294:Narcotics; D003042:Cocaine