title: Unique sequence of a high molecular weight myosin light chain kinase is involved in interaction with actin cytoskeleton.
abstract: Myosin light chain kinase (MLCK) is the key regulator of cell motility and smooth muscle contraction in higher vertebrates. We searched for the features of the high molecular weight MLCK (MLCK-210) associated with its unique N-terminal sequence not found in a more ubiquitous lower molecular weight MLCK (MLCK-108). MLCK-210 demonstrates stronger association with the Triton-insoluble cytoskeletons than MLCK-108, suggesting the role for this sequence in subcellular targeting. Indeed, the expressed unique domain of MLCK-210 binds and bundles F-actin in vitro and colocalises with the microfilaments in transfected cells reproducing endogenous MLCK-210 distribution. Thus, MLCK-210 features an extensive actin binding interface and, perhaps, acts as an actin cytoskeleton stabiliser.
authors.affiliation: Laboratory of Cell Motility, Institute of Experimental Cardiology, Russian Cardiology Research Center, 3rd Cherepkovskaya st., 15a, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Kudryashov
authors.forename: M V
authors.initials: MV
authors.lastname: Chibalina
authors.forename: K G
authors.initials: KG
authors.lastname: Birukov
authors.forename: T J
authors.initials: TJ
authors.lastname: Lukas
authors.forename: J R
authors.initials: JR
authors.lastname: Sellers
authors.forename: L J
authors.initials: LJ
authors.lastname: Van Eldik
authors.forename: D M
authors.initials: DM
authors.lastname: Watterson
authors.forename: V P
authors.initials: VP
authors.lastname: Shirinsky
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D002645:Chickens; D003599:Cytoskeleton / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry* / Q000378:metabolism*; D020033:Protein Isoforms; D011817:Rabbits; D014422:Turkeys
chemical_list: D000199:Actins; D008164:Luminescent Proteins; D020033:Protein Isoforms; D049452:Green Fluorescent Proteins; D009219:Myosin-Light-Chain Kinase