Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR.
A primer pair (p289/290) based on the RNA polymerase sequence of 25 prototype and currently circulating strains of human caliciviruses (HuCVs) was designed for the detection of both Norwalk-like caliciviruses (NLVs) and Sapporo-like caliciviruses (SLVs) by reverse transcription-polymerase chain reaction (RT-PCR). This primer pair produces RT-PCR products of 319 bp for NLVs and 331 bp for SLVs. The usefulness of this primer pair was shown by its detection of prototype NLVs (Norwalk, Snow Mountain, Hawaii and Mexico viruses) and SLVs (Sapporo/82, Hou/86, Hou/90 and Lon/92) and currently circulating strains of NLVs and SLVs in children and adults. This primer pair also detected more viruses in either NLV or SLV genera than previously designed primers. This primer pair is useful for broad detection of HuCVs for clinical and epidemiologic studies as well as for environmental monitoring.
Jiang
X
X
Center for Pediatric Research, Children's Hospital of The King's Daughters, Eastern Virginia Medical School, Norfolk 23510-1001, USA. jjiang@chkd.com
Huang
P W
PW
Zhong
W M
WM
Farkas
T
T
Cubitt
D W
DW
Matson
D O
DO
D000328:Adult; D001483:Base Sequence; D002139:Caliciviridae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D002648:Child; D017931:DNA Primers / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004196:Disease Outbreaks; D005069:Evaluation Studies as Topic; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D006801:Humans; D017720:Molecular Epidemiology; D009663:Norwalk virus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods*
D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases