The male determinant of self-incompatibility in Brassica.
In the S locus-controlled self-incompatibility system of Brassica, recognition of self-related pollen at the surface of stigma epidermal cells leads to inhibition of pollen tube development. The female (stigmatic) determinant of this recognition reaction is a polymorphic transmembrane receptor protein kinase encoded at the S locus. Another highly polymorphic, anther-expressed gene, SCR, also encoded at the S locus, fulfills the requirements for the hypothesized pollen determinant. Loss-of-function and gain-of-function studies prove that the SCR gene product is necessary and sufficient for determining pollen self-incompatibility specificity, possibly by acting as a ligand for the stigmatic receptor.
Schopfer
C R
CR
Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA.
Nasrallah
M E
ME
Nasrallah
J B
JB
D000483:Alleles; D000595:Amino Acid Sequence; D001937:Brassica / Q000235:genetics / Q000502:physiology*; D003545:Cysteine / Q000737:chemistry; D017343:Genes, Plant; D018525:Germination; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006239:Haplotypes; D008024:Ligands; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D018514:Plant Structures / Q000235:genetics / Q000502:physiology; D011058:Pollen / Q000235:genetics / Q000502:physiology*; D011110:Polymorphism, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014170:Transformation, Genetic
D006023:Glycoproteins; D008024:Ligands; D010940:Plant Proteins; C401308:SCR protein, Brassica oleracea; C064156:self-incompatibility locus specific glycoprotein, Brassica; D011494:Protein Kinases; C079832:S-receptor kinase; D003545:Cysteine