Recent developments in DNA sequencing by capillary and microdevice electrophoresis.
The present review covers papers published in the years 1997 and 1998 on DNA sequencing by capillary and microdevice electrophoresis. The article does not include other electrophoretic DNA applications such as analysis of oligonucleotides, genotyping, and mutational analysis. Capillary gel electrophoresis (CGE) is starting to become a viable competitor to slab gel electrophoresis for DNA sequencing. Commercially available multicapillary array sequencers are now entering sequencing facilities which to date have totally relied on traditional slab gel technology. CGE research on DNA sequencing therefore becomes increasingly concerned with the critical task of fine-tuning the operational parameters to create robust sequencing systems. Electrophoretic microdevices are being considered the next technological step in DNA sequencing by electrophoresis.
Schmalzing
D
D
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. schmalzing@wi.mit.edu
Koutny
L
L
Salas-Solano
O
O
Adourian
A
A
Matsudaira
P
P
Ehrlich
D
D
D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods* / Q000639:trends
D004396:Coloring Agents; D004247:DNA