DNA vaccination of mice with plasmid expressing human papillomavirus 6 major capsid protein L1 elicits type-specific antibodies neutralizing pseudovirions constructed in vitro.
Human papillomavirus 6 (HPV 6) causes benign condylomata. As a model for HPV vaccine development, we tested a HPV 6 DNA vaccine candidate, constructed by subcloning the major capsid protein (L1) gene into an expression plasmid having the cytomegalovirus promoter, for its immunogenicity in BALB/c mice. Three intracutaneous inoculations of the plasmid with a gene gun at 2-week intervals elicited anti-L1 serum antibodies. The antibodies were found to recognize highly type-specific, conformation-dependent epitopes, including those to neutralize pseudovirions capable of inducing beta-galactosidase in infected monkey COS-1 cells. The data support the idea that immunization with DNA capable of expressing HPV L1 can be used as an HPV vaccine strategy for humans.
Matsumoto
K
K
Division of Molecular Genetics, National Institute of Infectious Diseases, Tokyo, Japan.
Kawana
K
K
Yoshikawa
H
H
Taketani
Y
Y
Yoshiike
K
K
Kanda
T
T
D000818:Animals; D000917:Antibody Formation; D019470:Biolistics; D002213:Capsid / Q000276:immunology / Q000378:metabolism*; D036022:Capsid Proteins; D000939:Epitopes; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000378:metabolism*; D027383:Papillomaviridae / Q000276:immunology*; D030361:Papillomavirus Infections / Q000276:immunology* / Q000517:prevention & control; D014412:Tumor Virus Infections / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins; D001616:beta-Galactosidase / Q000235:genetics
D036022:Capsid Proteins; D000939:Epitopes; D009856:Oncogene Proteins, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; C091560:L1 protein, Human papillomavirus type 6; D001616:beta-Galactosidase