Glucosaminyl N-deacetylase mRNA expression in diabetic rats.
Glucosaminyl N-deacetylase is a key enzyme in the synthesis of heparan sulphate. N-deacetylase activity is inhibited in experimental diabetes. The mechanism by which diabetes influences N-deacetylase activity is unknown. Using reverse transcription PCR we quantified hepatic N-deacetylase mRNA in normal and streptozotocin diabetic rats. A negative correlation was found between final blood glucose and N-deacetylase mRNA expression in diabetic rats, r = -0.44, p < 0.05. N-deacetylase mRNA expression was also correlated to N-deacetylase activity (r = 0.48, p < 0.05). N-deacetylase:cyclophilin mRNA ratio was not significantly affected by diabetes, 0.26 (0.2-0.35) vs 0.31 (0.16-0.5), p =0.48, in 10 weeks and 0.21 (0.15-0.37) vs 0.27 (0.19-0.32), p = 0.65, in 8 months rats, diabetic and normal rats respectively, median (interquartile range). The negative correlation between blood glucose and N-deacetylase mRNA in diabetic rats suggests however that altered gene expression may contribute to the altered N-deacetylase activity seen in experimental diabetes.
Oturai
P
P
Steno Diabetes Center, Gentofte, and Novo Nordisk, Health Care Discovery, Bagsvaerd, Denmark. p.oturai@dadlnet.dk
Deckert
M
M
Rolin
B
B
Jensen
T
T
Kofoed-Enevoldsen
A
A
D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008297:Male; D019696:Peptidylprolyl Isomerase / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001786:Blood Glucose; D012333:RNA, Messenger; D000581:Amidohydrolases; C023079:N-acetylglucosamine deacetylase; D019696:Peptidylprolyl Isomerase