Cytochrome c redistribution in apoptosis of guinea pig vestibular hair cells.
We examined the cellular location of cytochrome c in healthy and in streptomycin-damaged vestibular hair cells by immunohistochemistry. Staining for cytochrome c revealed an immunostain pattern that was consistent with a mitochondrial distribution of this enzyme in healthy hair cells, while in hair cells affected by aminoglycosides, a diffuse distribution in the cytoplasm was found together with apoptotic nuclear morphology. These findings suggest that redistribution of cytochrome c is involved in the pathway of apoptosis in vestibular hair cells induced by aminoglycosides.
Nakagawa
T
T
Department of Otorhinolaryngology, Yodogawa Christian Hospital, 2-9-26 Awaji, Higashiyodogawaku, 533-0032, Osaka, Japan. a195112@ych.or.jp
Yamane
H
H
D000818:Animals; D000900:Anti-Bacterial Agents; D017209:Apoptosis / Q000502:physiology*; D003574:Cytochrome c Group / Q000378:metabolism*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects / Q000378:metabolism*; D013307:Streptomycin
D000900:Anti-Bacterial Agents; D003574:Cytochrome c Group; D013307:Streptomycin