title: Applications of capillary electrophoresis in biotechnology.
abstract: Capillary electrophoresis (CE)-related techniques are increasingly being used as a matter of routine practice in the biotechnology discipline. Since recombinant DNA-derived proteins and the antisense oligonucleotides constitute a large portion of the applications of these techniques, they have been emphasized in this review. Analyses by CE of Escherichia coli-derived proteins and glycosylated proteins derived from mammalian cell cultures are summarized, as well as those of the carbohydrate chains that have been enzymatically removed from the protein. Applications of CE in the analysis of the antisense oligonucleotides for the determination of purity and the analytical studies on the metabolism of these modified oligonucleotides, by CE are reviewed. The literature mainly covers the period from 1996.
authors.affiliation: Lilly Research Laboratories, A Division of Eli Lilly and Co., Lilly Corporate Center, Indianapolis, IN 46285, USA. allagu@lilly.com
authors.forename: A L
authors.initials: AL
authors.lastname: Lagu
mesh_terms: D016375:Antisense Elements (Genetics) / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D001709:Biotechnology / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis; D006031:Glycosylation; D009844:Oligosaccharides / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D016375:Antisense Elements (Genetics); D006023:Glycoproteins; D009844:Oligosaccharides; D011994:Recombinant Proteins