Effect of clofibrate on malic enzyme and leptin mRNAs level in rat brown and white adipose tissue.
Two previous studies have reported contradictory results regarding the effect of fibrates treatment on obese (ob) gene expression in rodents. The purpose of the present study was to reinvestigate this issue. We examined the effect of clofibrate (fibrate derivative) administration for 14 days to rats on malic enzyme (as an adequate control of fibrates action) and leptin mRNAs level in the white and brown adipose tissues (WAT and BAT, respectively). The malic enzyme activity and malic enzyme mRNA level in white adipose tissue increased significantly after clofibrate feeding. In brown adipose tissue, the drug treatment resulted in depression of malic enzyme activity and malic enzyme mRNA level. Under the same conditions, leptin mRNA level did not change in these tissues. The results presented in this paper provide further evidence that the clofibrate (activator of peroxisome proliferator activated receptor alpha), feeding is without effect on ob gene expression in rat white and brown adipose tissue. Furthermore, the present study demonstrates that clofibrate causes opposite effects on malic enzyme gene expression in WAT (up-regulation) and BAT (down-regulation).
Kochan
Z
Z
Department of Biochemistry, Medical University of Gdansk, Poland.
Karbowska
J
J
Swierczynski
J
J
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002994:Clofibrate / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D020738:Leptin / Q000235:genetics*; D008291:Malate Dehydrogenase / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000187:drug effects*
D020738:Leptin; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D008291:Malate Dehydrogenase; D002994:Clofibrate