Postnatal ontogeny of GTP binding protein in the human frontal cortex.
The postnatal development of G protein in membrane preparations from frontal cortex regions in postmortem brains of various ages was investigated by immunoblotting with polyclonal antibodies against several specific G protein subtypes (the short and long form of Galphas(:Gs), Galphai1.2(:Gi), Galphao(:Go) and Galphaq/11(:Gq)) and tubulinbeta, and functional photoaffinity GTP binding. The amounts of Go showed steep increases at about 2 years, and there were similar tendency about Gs, Gi1.2 and Gq/11. Moreover, tubulinbeta was constant with development. The guanine nucleotide binding of Gs, Gi and Go also transiently increased at about the age of 2 years but the ratio of Gs to Gi.o was unchanged. Our results might have relevance for developmental neuroplasticity in signal transduction.
Ozawa
H
H
Department of Neuropsychiatry, School of Medicine, Sapporo Medical University, Japan. ozawa@sapmed.ac.jp
Ukai
W
W
Kornhuber
J
J
Yamaguchi
T
T
Froelich
L
L
Ikeda
H
H
Saito
T
T
Riederer
P
P
D000293:Adolescent; D000328:Adult; D000345:Affinity Labels / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism / Q000502:physiology*; D001386:Azides / Q000378:metabolism; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D005625:Frontal Lobe / Q000254:growth & development* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007223:Infant; D008566:Membranes / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D010865:Pilot Projects; D014404:Tubulin / Q000378:metabolism / Q000502:physiology
D000345:Affinity Labels; D001386:Azides; D014404:Tubulin; C031907:GTP gamma-4-azidoanilide; D006160:Guanosine Triphosphate; D020962:Heterotrimeric GTP-Binding Proteins