title: The cancer chemopreventive agent resveratrol is incorporated into model membranes and inhibits protein kinase C alpha activity.
abstract: Resveratrol is a phytoalexin found in grapes and other foods that cancer chemopreventive and other biological activities have been attributed recently. We report that resveratrol is able to incorporate itself into model membranes in a location that is inaccessible to the fluorescence quencher, acrylamide. Differential scanning calorimetry revealed that resveratrol considerably affected the gel to liquid-crystalline phase transition of multilamellar vesicles made of phosphatidylcholine/phosphatidylserine and increased the temperature at which the fluid lamellar to H(II) inverted hexagonal transition took place in multilamellar vesicles made of 1,2-dielaidoyl-sn-phosphatidylethanolamine. Such a transition totally disappeared at 2.5 mM of resveratrol (resveratrol/lipid molar ratio of 2:1). This effect on 1, 2-dielaidoyl-sn-phosphatidylethanolamine polymorphism was confirmed through (31)P-NMR, which showed that an isotropic peak appeared at high temperature instead of the H(II)-characteristic peak of 42 mM of resveratrol (resveratrol/lipid molar ratio of 1.5:1). Finally, resveratrol inhibited PKCalpha when activated by phosphatidylcholine/phosphatidylserine vesicles with an IC(50) of 30 microM, whereas when the enzyme was activated by Triton X-100 micelles the IC(50) was 300 microM. These results indicate that the inhibition of PKCalpha by resveratrol can be mediated, at least partially, by membrane effects exerted near the lipid-water interface.
authors.affiliation: Edificio de Veterinaria, Universidad de Murcia, Apartado Postal 4021, Murcia, E-30080, Spain.
authors.forename: J
authors.initials: J
authors.lastname: García-García
authors.forename: V
authors.initials: V
authors.lastname: Micol
authors.forename: A
authors.initials: A
authors.lastname: de Godos
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Fernández
mesh_terms: D020106:Acrylamide / Q000378:metabolism; D000818:Animals; D016588:Anticarcinogenic Agents / Q000378:metabolism* / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D004058:Diffusion / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005453:Fluorescence; D020128:Inhibitory Concentration 50; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity / Q000187:drug effects; D008567:Membranes, Artificial; D008823:Micelles; D017830:Octoxynol / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051571:Protein Kinase C-alpha; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism* / Q000494:pharmacology*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D016588:Anticarcinogenic Agents; D007527:Isoenzymes; D008081:Liposomes; D008567:Membranes, Artificial; D008823:Micelles; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D013267:Stilbenes; D020106:Acrylamide; C020888:1,2-dielaidoylphosphatidylethanolamine; D017830:Octoxynol; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D000077185:Resveratrol