[Vector construction for embryonic stem cell gene targeting of site specific point mutation mouse coagulation factor IX gene].
OBJECTIVE
To construct the recombinant vectors for embryonic stem(ES) cell gene targeting which contain the mouse coagulation factor IX (F IX) gene modified by PCR site-directed mutagenesis.


METHODS
Three site specific point mutations were introduced into exon 8 of mouse F IX gene respectively. The replacement targeting vectors were constructed and transfected into ES cells. The drug-resistant cell clones were picked after drug selection.


RESULTS
The construction of targeting vectors was successful and several drug-resistant ES cell clones were gained.


CONCLUSIONS
The site specific point mutation system can modify human gene in vitro more accurately. It is useful in the setting up of animal models.
Li
J
J
Department of Medical Genetics, The Second Military Medical University, Shanghai 200433 P.R.China. LiJian-1996@Yahoo.com
Dai
X
X
Yang
H
H
Fu
J
J
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology*; D005164:Factor IX / Q000235:genetics*; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D017354:Point Mutation; D013234:Stem Cells / Q000378:metabolism*
D005164:Factor IX