title: IS1626, a new IS900-related Mycobacterium avium insertion sequence.
abstract: An insertion sequence designated IS1626 was isolated and characterized from a Mycobacterium avium clinical strain. IS1626 was detected by high-stringency hybridization with the pMB22/S12 probe from IS900 of Mycobacterium paratuberculosis. IS1626 is 1418 bp in size and has a G+C content of 65 mol%. It has neither terminal inverted repeats nor flanking direct repeats. Analysis of three IS1626 insertion sites in the M. avium strain and the corresponding potential insertion sites in two IS1626-free M. avium strains indicated a consensus sequence of CATGCN(4-5)TCCTN(2)G for IS1626 insertion. In the three clones examined, IS1626 has the same orientation with respect to this target site. IS1626 has two major ORFs. ORF1179 encodes a predicted protein of 393 amino acids. ORF930, on the complementary strand of ORF1179, encodes a protein of 310 amino acids. The Shine-Dalgarno sequence for ORF930 is partially located in the flanking region, similar to other IS900-related elements. Analysis of the comparable features of insertion sequences and their variable occurrence in related organisms is useful for studying the evolution of these elements and their hosts.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Puyang
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
authors.forename: Karen
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
authors.forename: Corey
authors.initials: C
authors.lastname: Pawlichuk
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
authors.forename: Dennis Y
authors.initials: DY
authors.lastname: Kunimoto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D016384:Consensus Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000235:genetics*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial