Recombinant forms of the neurotrophic factor pigment epithelium-derived factor activate cellular metabolism and inhibit proliferation of the RAW macrophage cell line.
Recombinant forms of the neurotrophic factor pigment epithelium-derived factor (PEDF) activate metabolism of RAW macrophage cells while simultaneously inhibiting their proliferation. The recombinant forms (rPEDF) acted with EC(50)s of 0.1-1 nM while full-length native bovine PEDF was inactive. Urea, which is the buffer used to extract recombinant PEDF, stimulated RAW cell proliferation, the first report of an effect of urea on non-kidney cells. PEDF acted within 12 h and its effects persisted up to 72 h with continuous exposure. Although rPEDF had no direct action on glioma cell lines, it increased the amount of a soluble factor released by RAW cells which was capable of blocking glioma cell division. Thus PEDF may function as a neuroimmune modulator, affecting both neural and immune system cells.
Cohen
J
J
Department of Pharmacology, Howard University College of Medicine, Washington, DC, USA.
Sugita
Y
Y
Chader
G J
GJ
Schwartz
J P
JP
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D001932:Brain Neoplasms; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005136:Eye Proteins; D005909:Glioblastoma; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D015843:Serpins / Q000235:genetics / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism; D014508:Urea / Q000494:pharmacology; D000099174:Pigment Epithelium-Derived Factor
D001798:Blood Proteins; D016895:Culture Media, Serum-Free; D005136:Eye Proteins; D009414:Nerve Growth Factors; D011506:Proteins; D011994:Recombinant Proteins; D015843:Serpins; D014508:Urea; D000099174:Pigment Epithelium-Derived Factor