[Transformation of common wheat (Triticum aestivum L.) with herbicide-resistant EPSPs gene].
The herbicide-resistant EPSPs (5-enolpyruvylshikimate-3-phosphate synthase) gene was transformed into about 1,000 young spikes and 800 young embryos of wheat variety, Jinghua 1, with gene gun. Thirty-eight and four regenerated plants were obtained respectively screened with glyphosate. All regenerated plants were analysed by PCR and/or Southern blotting. The results indicated that EPSPs gene was integrated stably into the genome of Jinghua 1, and some of the transformants showed fertile. So herbicide-resistant EPSPs gene could be used as selective marker in the transformation of monocotyledon cereal crops, such as wheat.
Chen
L H
LH
Dept. of Plant Genetics and Breeding China Agricultural University Beijing.
Wang
X W
XW
Zhang
W J
WJ
Zhang
X D
XD
Hu
D F
DF
Liu
G T
GT
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D015139:Blotting, Southern; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics*
D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase