An overall perspective of X-ray damage to a DNA oligomer mediated by reactive oxygen species.
The products produced by X irradiation of an oxygenated aqueous solution containing d(CpApTpG) were analyzed by NMR spectroscopy and mass spectrometry. Thirteen different base modifications were detected, including a novel product formed by the addition of oxygen to guanine. Seven different strand break products were identified, including strands having 5'-phosphoryl groups, 3'-phosphoryl groups and groups having 3'-phosphoglycolates as termini. The products produced in largest yield contained base modifications: Pyrimidine bases degraded to a formamido moiety, the 8-oxo-7,8-dihydroguanine (8-oxoguanine) lesion, and double base lesions in which both the 8-oxo-7,8-dihydroguanine lesion and a formamido remnant are present.
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Biophysics Department, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Dawidzik
J B
JB
Wallace
J C
JC
Hauer
C R
CR
Stack
R F
RF
Rajecki
M J
MJ
Budzinski
E E
EE
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species; D012996:Solutions; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014965:X-Rays
D009838:Oligodeoxyribonucleotides; D017382:Reactive Oxygen Species; D012996:Solutions; D004247:DNA