title: Molecular characterization and in situ localization of a full-length cyclic nucleotide-gated channel in rat brain.
abstract: Ion channels gated directly by cyclic nucleotides are required for the transduction of sensory signals in photoreceptor cells and olfactory cells. Cyclic nucleotide-gated (CNG) channels may also be expressed in the central nervous system because partial transcripts that share homology with CNG channels have been found therein. We have now isolated and cloned a full-length CNG channel cDNA from adult rat brain. The sequence is identical to that of the alpha-subunit originally found in the olfactory epithelium (CNCalpha3). In situ hybridization, using probes specific for the CNCalpha3 mRNA, suggest that this channel is expressed widely in the rat brain, albeit mostly at relatively low levels. Certain neuronal populations, however, such as deep cerebellar nuclei, olfactory bulb mitral cells and cerebellar Purkinje neurons, appeared specially enriched. The study demonstrates for the first time that a full-length CNG channel mRNA is expressed in the brain, supporting the possibility that CNG channels are involved in central neural communication and plasticity. The sequence reported in this paper has been deposited in the GenBank data base (accession no. AF126808).
authors.affiliation: The Wolfson Institute for Biomedical Research, University College London, UK. paul-j-strijbos@sbphrd.com
authors.forename: P J
authors.initials: PJ
authors.lastname: Strijbos
authors.forename: G D
authors.initials: GD
authors.lastname: Pratt
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: I G
authors.initials: IG
authors.lastname: Charles
authors.forename: J
authors.initials: J
authors.lastname: Garthwaite
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D054815:Cyclic Nucleotide-Gated Cation Channels; D018076:DNA, Complementary / Q000235:genetics; D017403:In Situ Hybridization; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism; D017966:Pyramidal Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D014018:Tissue Distribution
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; D018076:DNA, Complementary; D007473:Ion Channels; D009712:Nucleotides, Cyclic; D012333:RNA, Messenger