title: [Cytophotometric investigations in recurrent gliomas: correlation of DNA-parameters with WHO-grade, proliferation index and recurrence-free interval].
abstract: DNA-cytophotometry is one of the methods that may contribute to a more precise evaluation of the biological behaviour of tumours in addition to the WHO-classification. In this study 121 tumour specimens of 50 patients suffering from gliomas with one or up to three recurrencies were investigated. In all cases the histological type and WHO-grade and the Ki-67/MIB1 labeling index were determined. DNA cytophotometry was performed after single cell preparation on Feulgen-stained preparations, and the following parameters were calculated: stemline ploidy, 5c-exceeding rate, and 2c-deviation index. Statistical evaluation revealed a highly significant correlation between recurrence-free interval and WHO-grade only. The DNA parameters, however, furnished additional information about increasing genetic instability in the majority of the recurrencies independently of changes in the WHO-grade. They thus seem to be useful as additional parameters for the determination of glioma progression.
authors.affiliation: Abteilung für Neuropathologie, Institut für Pathologie, Universität Leipzig.
authors.forename: W
authors.initials: W
authors.lastname: Schneider
authors.forename: S
authors.initials: S
authors.lastname: Kellermann
authors.forename: H
authors.initials: H
authors.lastname: Nenning
authors.forename: D R
authors.initials: DR
authors.lastname: Thal
authors.forename: R
authors.initials: R
authors.lastname: Schober
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002455:Cell Division / Q000235:genetics*; D003592:Cytophotometry; D004273:DNA, Neoplasm / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005910:Glioma / Q000145:classification / Q000235:genetics / Q000473:pathology*; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000145:classification / Q000235:genetics / Q000473:pathology*; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm; D019394:Ki-67 Antigen
vernacular_title: Zytophotometrische Untersuchungen an Rezidivgliomen. Korrelation der DNA-Parameter mit dem WHO-Grad, dem Proliferationsindex und der Länge der rezidivfreien Intervalle.