Flicker image comparison of 2-D gel images for putative protein identification using the 2DWG meta-database.
With the availability of two-dimensional (2-D) gel electrophoresis databases that have many characterized proteins, it may be possible to compare a researcher's gel images with those in relevant databases. This may lead to the putative identification of unknown protein spots in a researcher's gel with those characterized in a given database, saving the researcher time and money by suggesting monoclonal antibodies to try in confirming these identifications. We have developed two tools to help with this comparison: (1) Flicker, http:/(/)www.lecb.ncifcrf.gov/flicker/, a Java applet program running in the researcher's Web browser, to visually compare their gels against gels on the Internet; and (2) the 2DWG meta-database, http:/(/)www.lecb.ncifcrf.gov/2dwgDB /, a searchable database of locations of 2-D electrophoretic gel images found on the Internet. Recent additions to Flicker allow users to click on a protein spot in a gel that is linked to a federated 2D gel database, such as SWISS-2DPAGE, and have it retrieve a report from that Web database for that protein.
Lemkin
P F
PF
IPS/LECB NCI/FCRDC, Frederick, MD 21702, USA. lemkin@ncifcrf.gov
Thornwall
G
G
D003626:Data Display; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D016247:Information Storage and Retrieval; D020407:Internet; D011506:Proteins / Q000032:analysis*; D012984:Software; D014584:User-Computer Interface
D011506:Proteins