Distribution and subcellular localization of acylpeptide hydrolase and acylase I along the hog gastro-intestinal tract.
The distribution of acylase I and acylpeptide hydrolase along the hog small intestine was investigated. No significant changes in their respective specific activity was found when the intestine was cut off and divided into eight segments (taken every 200 cm) so as to specifically study the duodenum, jejunum and ileum. Upon performing subcellular fractionation of hog enterocytes, it was observed that acylpeptide hydrolase is a soluble enzyme, while acylase I is essentially a soluble protein accounting for only 5% of the activity associated with the whole membrane fraction. The membrane-bound acylase I was neither solubilized by phosphatidylinositol-specific phospholipase C from Bacillus cereus nor by detergents which are commonly used to solubilize alkaline phosphatase, a glycosylphosphatidylinositol-anchored protein. When a phase separation was carried out in Triton X-114, all the anchored-membrane proteins of the intestinal membranes were located in the detergent-rich phase, while acylase I was present in the detergent-poor phase. Finally, the immunolabeling of intestinal cells with specific antibodies definitively established the cytoplasmic localization of acylase I. Acylpeptide hydrolase and acylase I therefore both are located in the enterocyte cytoplasm.
Giardina
T
T
Laboratoire de Biochimie et Biologie de la Nutrition, CNRS-ESA 6033, Faculté des Sciences et Techniques Saint-Jérôme, 13397 Marseille, cedex 20 France.
Biagini
A
A
Massey-Harroche
D
D
Puigserver
A
A
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D004064:Digestive System / Q000201:enzymology*; D004386:Duodenum / Q000201:enzymology; D020689:Exopeptidases / Q000378:metabolism*; D007082:Ileum / Q000201:enzymology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D010447:Peptide Hydrolases; D013347:Subcellular Fractions / Q000201:enzymology; D013552:Swine; D014018:Tissue Distribution
D020689:Exopeptidases; D010447:Peptide Hydrolases; C038220:acylaminoacyl-peptidase; D000581:Amidohydrolases; C019672:aminoacylase I