title: Chromatographic properties of reversed-phase stationary phases under pressure- and electro-driven conditions.
abstract: Seven different reversed-phase (RP) stationary phases were examined under high-performance liquid chromatographic (pressure-driven, HPLC), and capillary electrochromatographic (electro-driven, CEC) conditions. Characterization of the stationary phases was performed following well-established test procedures providing a number of distinct column descriptors: hydrophobicity, hydrophobic selectivity and silanol activity. These parameters were used to describe the behavior of the RP-columns under both HPLC and CEC conditions. It is shown that chromatographic characteristics of porous RP-phases greatly depend on the mode of operation. By contrast, column descriptors of a non-porous viz. solid RP-phase material hardly differed for HPLC and CEC conditions.
authors.affiliation: Eindhoven University of Technology, Department of Instrumental Analysis, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Jiskra
authors.forename: C A
authors.initials: CA
authors.lastname: Cramers
authors.forename: M
authors.initials: M
authors.lastname: Byelik
authors.forename: H A
authors.initials: HA
authors.lastname: Claessens
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*