CD8+TCR+ and CD8+TCR- cells in whole bone marrow facilitate the engraftment of hematopoietic stem cells across allogeneic barriers.
Although purified hematopoietic stem cells (HSC) are sufficient to engraft irradiated allogeneic recipients, bone marrow (BM) contains other cells that facilitate engraftment. Here, several candidate facilitators were tested by cotransplantation with HSC. Both TCR+ and TCR- CD8alpha+ BM subpopulations have facilitative potential. CD8+TCR+ cells are typical T lymphocytes. CD8+TCR- facilitators are CD3 , not CD3+, have a granular morphology, and are CD8beta- and CD11c+; they share phenotypic characteristics with CD8(alpha)alpha lymphoid dendritic cells and veto cells. We also demonstrate that lytic function is nqt necessary for facilitation and that the CD8alpha molecule is either important for facilitation or in the development of facilitators.
Gandy
K L
KL
Department of Pathology and Developmental Biology, Stanford University Medical Center, Stanford University, California 94305, USA. gandy001@mc.duke.edu
Domen
J
J
Aguila
H
H
Weissman
I L
IL
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology* / Q000637:transplantation; D003602:Cytotoxicity, Immunologic; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D006183:H-2 Antigens / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000502:physiology*; D014184:Transplantation, Homologous / Q000276:immunology*
D006183:H-2 Antigens; D011948:Receptors, Antigen, T-Cell