Molecular characterization and distribution of a 145-bp tandem repeat family in the genus Populus.
This report aims to describe the identification and molecular characterization of a 145-bp tandem repeat family that accounts for nearly 1.5% of the Populus genome. Three members of this repeat family were cloned and sequenced from Populus deltoides and P. ciliata. The dimers of the repeat were sequenced in order to confirm the head-to-tail organization of the repeat. Hybridization-based analysis using the 145-bp tandem repeat as a probe on genomic DNA gave rise to ladder patterns which were identified to be a result of methylation and (or) sequence heterogeneity. Analysis of the methylation pattern of the repeat family using methylation-sensitive isoschizomers revealed variable methylation of the C residues and lack of methylation of the A residues. Sequence comparisons between the monomers revealed a high degree of sequence divergence that ranged between 6% and 11% in P. deltoides and between 4.2% and 8.3% in P. ciliata. This indicated the presence of sub-families within the 145-bp tandem family of repeats. Divergence was mainly due to the accumulation of point mutations and was concentrated in the central region of the repeat. The 145-bp tandem repeat family did not show significant homology to known tandem repeats from plants. A short stretch of 36 bp was found to show homology of 66.7% to a centromeric repeat from Chironomus plumosus. Dot-blot analysis and Southern hybridization data revealed the presence of the repeat family in 13 of the 14 Populus species examined. The absence of the 145-bp repeat from P. euphratica suggested that this species is relatively distant from other members of the genus, which correlates with taxonomic classifications. The widespread occurrence of the tandem family in the genus indicated that this family may be of ancient origin.
Rajagopal
J
J
Biotechnology Division, TERI, New Delhi, India.
Das
S
S
Khurana
D K
DK
Srivastava
P S
PS
Lakshmikumaran
M
M
D001483:Base Sequence; D015139:Blotting, Southern; D019175:DNA Methylation; D018744:DNA, Plant; D004276:DNA, Satellite / Q000032:analysis; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D020080:Tandem Repeat Sequences; D014197:Trees / Q000235:genetics*
D018744:DNA, Plant; D004276:DNA, Satellite