Determination of amoxicillin in trout by liquid chromatography with UV detection after derivatization.
A liquid chromatographic (LC) method based on solid-phase extraction was developed for determination of amoxicillin in muscle tissue of rainbow trout. The compound was extracted in an aqueous solution by precipitation of organic material with a mixture of sulfuric acid and sodium tungstate. The extract was processed by solid-phase extraction on an end-capped phenyl sorbent, and concentrated on a divinylbenzene-co-N-vinylpyrrolidone polymeric sorbent. The extract was derivatized and analyzed by reversed-phase gradient LC on a C18 column with UV detection at 323 nm. The method detection limit was 2.9 micrograms/kg. Mean recovery in muscle was 80.5% (range 10-200 micrograms/kg). The method was applied to fillets from trout offered feed containing amoxicillin in an aquaculture pilot plant. Amoxicillin was detected in muscle tissue shortly after administration but not 3 weeks later. The relative repeatability standard deviation for incurred residues in muscle tissue was 6.4% (range 11-143 micrograms/kg).
Sørensen
L K
LK
Steins Laboratorium, Brørup, Denmark.
Hansen
H
H
Snor
L
L
D000327:Adsorption; D000658:Amoxicillin / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis*; D011786:Quality Control; D012680:Sensitivity and Specificity; D014337:Trout
D010406:Penicillins; D000658:Amoxicillin