Localized adhesion molecule expression and circulating LFA-3 levels in adult and early onset forms of periodontitis.
Because of their importance in mediating cellular interactions in chronic inflammatory diseases, this study has examined the expression of a number of adhesion molecules in adult (n=11), generalized early onset (n=5) and localized early onset (n=2) forms of periodontitis. In comparison with immunostaining profiles of cryostat sections of healthy gingival tissue (n=7), the beta 1 integrins VLA-1, VLA-2 and VLA-4 were found to be up-regulated in periodontitis, with VLA-6 being markedly elevated. Although only small differences were observed in ICAM-1 and LFA-3 expression in the gingival epithelium, there was particularly notable up-regulation of these adhesion molecules within the inflammatory infiltrates of the diseased tissues. However, there were no statistically significant differences between the serum levels of a soluble form of LFA-3 in periodontitis patients (n=47) compared with healthy control subjects (n=40), although the generalized early onset and adult periodontitis groups exhibited wider ranges of circulating LFA-3. These findings show that there is localized modulation of adhesion molecule expression in the chronic inflammatory periodontal diseases studied, but that the levels of LFA-3 in the circulation nevertheless remain unaffected.
Kirby
A C
AC
Department of Oral Medicine, Eastman Dental Institute for Oral Health Care Sciences, University of London, UK.
Griffiths
G
G
Gokbuget
A
A
Newman
H N
HN
Porter
S R
SR
Olsen
I
I
D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism; D018968:CD58 Antigens / Q000097:blood*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*
D018968:CD58 Antigens; D015815:Cell Adhesion Molecules