Inhibitory effect of pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters.
Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4. The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus. Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid polypeptide promoter through a sequence element located downstream of -138. The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349.
Campbell
S C
SC
Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, UK.
Cragg
H
H
Elrick
L J
LJ
Macfarlane
W M
WM
Shennan
K I
KI
Docherty
K
K
D000682:Amyloid / Q000037:antagonists & inhibitors* / Q000235:genetics; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D007329:Insulin Antagonists; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000378:metabolism; D051379:Mice; D051761:Paired Box Transcription Factors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D020033:Protein Isoforms; D051381:Rats; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*; D014162:Transfection
D000682:Amyloid; D018076:DNA, Complementary; D018398:Homeodomain Proteins; D007329:Insulin Antagonists; D058228:Islet Amyloid Polypeptide; C491709:PAX4 protein, human; D051761:Paired Box Transcription Factors; C110218:Pax4 protein, mouse; C491708:Pax4 protein, rat; D020033:Protein Isoforms; D014157:Transcription Factors