Central nervous system chemokine expression during Theiler's virus-induced demyelinating disease.
Theiler's murine encephalomyelitis virus is an endemic murine pathogen that induces a demyelinating disease of the central nervous system in susceptible mouse strains. The disease is characterized by central nervous system mononuclear cell infiltration and presents as chronic, progressive paralysis. The expression of CC and C-x-C chemokines in the central nervous system of Theiler's murine encephalomyelitis virus-infected mice was examined throughout the disease course by ELISA and RT - PCR analysis. Central nervous system expression of MCP-1 and MIP-1alpha protein was evident by day 11 post Theiler's murine encephalomyelitis virus infection of SJL mice and continued throughout disease progression. MIP-1alpha, RANTES, MCP-1, C10, IP-10, and MIP-1beta mRNA was specifically expressed in the central nervous system and not the periphery following Theiler's murine encephalomyelitis virus infection. This was associated with development of clinical disease. These data suggest that the expression of multiple chemokines at particular times following viral infection is associated with demyelinating disease.
Hoffman
L M
LM
Department of Pathology, Northwestern University Medical School, Chicago, Illinois, IL 60611, USA.
Fife
B T
BT
Begolka
W S
WS
Miller
S D
SD
Karpus
W J
WJ
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D018932:Chemokine CCL2 / Q000378:metabolism; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000378:metabolism; D054357:Chemokine CXCL10; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D019742:Chemokines, CC; D019743:Chemokines, CXC / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D051379:Mice; D011051:Poliomyelitis / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity; D017945:Theilovirus / Q000276:immunology*; D013997:Time Factors
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines; D019742:Chemokines, CC; D019743:Chemokines, CXC; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; C070959:Ccl6 protein, mouse