Chemotactic activities of avian lymphocytes.
Chicken lymphocytes, enriched chicken T and B lymphocytes, and a turkey B-lymphoblastoid cell line, the RP-9 cells, are used in the studies of chemotaxis in a Boyden-type chamber assay. The chemoattractants used are lipopolysaccharide, fMLP, interleukin-8, MIP1-beta, rabbit anti-chicken IgG and IgM. The results indicate that all these cells can migrate into the polycarbonate membranes in the absence of chemoattractants. When the chemoattractants are present, the numbers of migrating cells are greatly increased. It is, therefore, concluded that avian lymphocytes have the ability to migrate, and can respond to chemical signals which result in chemotaxis and accumulation of lymphocytes at the sites where the signals originate.
Lam
K M
KM
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis 95616, USA.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D002460:Cell Line; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000494:pharmacology; D002630:Chemotactic Factors / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D002645:Chickens; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011817:Rabbits; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
D054407:Chemokine CCL4; D018946:Chemokine CCL5; D002630:Chemotactic Factors; D016209:Interleukin-8; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine