title: ATP-gated ion channel expression in primary auditory neurones.
abstract: Extracellular ATP acts via ionotropic P2X receptors to mediate fast neurotransmission in the central and autonomic nervous systems. Recent data, including identification of P2X2 receptor mRNA expression by spiral ganglion neurones, suggests that purinergic signalling may influence auditory neurotransmission via ATP-gated ion channels assembled from these subunits. Expression of the P2X2 receptor was localized to the region of the spiral ganglion neurone synapses with the inner hair cells using a P2X2 receptor specific antiserum. Whole-cell patch clamping of neurones cultured from post-natal day 3-5 spiral ganglia demonstrated a heterogeneity of ATP-activated conductances, consistent with the functional expression of P2X2 receptor subunit isoforms along with possible co-expression of additional P2X receptor subunits. These data provide substantive support for a purinergic transmission element at the peripheral auditory synapse.
authors.affiliation: Department of Physiology, University of Auckland, New Zealand.
authors.forename: S G
authors.initials: SG
authors.lastname: Salih
authors.forename: G D
authors.initials: GD
authors.lastname: Housley
authors.forename: N P
authors.initials: NP
authors.lastname: Raybould
authors.forename: P R
authors.initials: PR
authors.lastname: Thorne
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D007150:Immunohistochemistry; D015640:Ion Channel Gating; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000502:physiology; D013136:Spiral Ganglion / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate