title: Determination of benomyl, diphenyl, o-phenylphenol, thiabendazole, chlorpyrifos, methidathion, and methyl parathion in oranges by solid-phase extraction, liquid chromatography, and gas chromatography.
abstract: A simple and rapid method was developed for determination of benomyl, diphenyl (DP), o-phenylphenol (OPP), thiabendazole (TBZ), chlorpyrifos, methidathion, and methyl parathion in whole oranges. These compounds were extracted from a mixture of samples and anhydrous sodium acetate with ethyl acetate. The ethyl acetate extract was concentrated and cleaned up by passing through tandem solid-phase extraction columns consisting of anion-exchange and primary/secondary amine bonded silica. The eluate was concentrated and volume was adjusted with methanol for subsequent liquid chromatography (LC) and gas chromatography (GC). Benomyl (as methyl-2-benzimidazole carbamate, MBC), DP, OPP, and TBZ residues were determined by LC with fluorescence detection. Recoveries at 3 fortified levels (0.1, 1, and 10 micrograms/g) ranged from 63.9 to 97.4%, with coefficients of variation (CVs) of 1.6 to 15.5%. Limits of detection (LODs) were 0.01 microgram/g for DP, OPP, TBZ and 0.05 microgram/g for benomyl. Chlorpyrifos, methidathion, and methyl parathion residues were determined by GC with flame photometric detection. Recoveries ranged from 90.4 to 97.0%, with CVs of 2.1 to 5.9%. LODs were 0.005 microgram/g for chlorpyrifos and methyl parathion, and 0.01 microgram/g for methidathion.
authors.affiliation: Institute of Food Hygiene, Japan Food Hygiene Association, Yaizu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: T
authors.initials: T
authors.lastname: Ninomiya
mesh_terms: D001542:Benomyl / Q000032:analysis; D001713:Biphenyl Compounds / Q000032:analysis; D004390:Chlorpyrifos / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D008743:Methyl Parathion / Q000032:analysis; D009946:Organothiophosphorus Compounds / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D013827:Thiabendazole / Q000032:analysis
chemical_list: D001542:Benomyl; D001713:Biphenyl Compounds; D004390:Chlorpyrifos; D005659:Fungicides, Industrial; D008743:Methyl Parathion; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues; D013827:Thiabendazole; C010574:biphenyl; C004369:2-phenylphenol; C005828:methidathion