title: The maize mutant polymitotic affects cell cycle events during microspore development
abstract: The maize (Zea mays L.) male-sterile mutant polymitotic (po) was analyzed utilizing in-vitro cell culture and immunocytochemistry methods to better understand the relationship between the mutant phenotype and cell cycle events during microspore development. Using a live meiocyte culture system, initiation and progression through abnormal post-meiotic cell cycles at the end of meiosis II was documented in the po mutant with a CCD camera and a computer image analysis system. Our results showed that premature chromosomal condensation precedes abnormal post-meiotic cell cycle progression in the po mutant at the end of meiosis II. Temporal analysis of the po mutant in-vitro revealed that unsynchronized post-meiotic divisions occurred immediately following the end of meiosis II and did not require interaction with the surrounding somatic tissue. Furthermore, the altered distribution of p34(cdc2) protein kinase from a nuclear to a cytoplasmic location was identified in tetrads during onset of the unsynchronized divisions in the po mutant. In contrast, a predominantly nuclear location of p34(cdc2) was observed during interphase of the wild-type tetrads at the same stage.
authors.affiliation: Department of Biology, University of Minnesota Duluth, 10 University Drive, Duluth, MN 55812-2496, USA.
authors.initials: KW
authors.lastname: Wolfe
authors.initials: Q
authors.lastname: Liu