An automated method for the detection of staphylococcal heat stable deoxyribonuclease in dairy products.
An automated sandwich immunoassay with specific polyclonal antibodies for the detection of Staphylococcus aureus thermostable nuclease (DNase) is described. To evaluate this assay, different quantities of purified S. aureus nuclease were added to dairy products. Additionally, staphylococcal counts and nuclease activity of milk samples inoculated with S. aureus were determined. Different extraction procedures were performed and compared. The results indicated that the automated test was a reliable method for detecting DNase activity in milk products. The procedure was completed in 2 h and detected 1 ng of DNase ml-1. Detection of the DNase was especially useful in cheeses and could be used to confirm positive enterotoxin results.
Meyrand
A
A
Unité de Microbiologie Alimentaire et Prévisionnelle, Ecole Nationale Vétérinaire de Lyon, France. hidaoa@vet-lyon.fr
Atrache
V
V
Bavai
C
C
Montet
M P
MP
Vernozy-Rozand
C
C
D000818:Animals; D015169:Colony Count, Microbial; D003611:Dairy Products / Q000382:microbiology*; D004768:Enterotoxins / Q000276:immunology / Q000378:metabolism; D004795:Enzyme Stability; D007118:Immunoassay / Q000379:methods*; D008836:Micrococcal Nuclease / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000378:metabolism
D004768:Enterotoxins; D008836:Micrococcal Nuclease