Heat stable lipase activity of thermotolerant bacteria from hot springs at Orissa, India.
Thermotolerant bacteria (35 in toto) isolated from three hot springs (Atri, Taptapani and Deuljhari, Orissa), were screened for lipase activities. Of these, nine strains of Bacillus spp. and three strains of Pseudomonas spp. showed heat stable lipase activity at 60 degrees C. The hydrolytic activity of these bacteria was tested using Tween-20 and Tween-80 as substrates at different temperatures using plate assay and titration techniques. The hydrolytic activity at different pH values and salt concentrations was investigated.
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Regional Medical Research Centre, ICMR, Chandra Sekharpur, Bhubaneswar, India.
D001407:Bacillus / Q000201:enzymology*; D004795:Enzyme Stability; D005618:Fresh Water; D006361:Heating; D007194:India; D008049:Lipase / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013210:Staphylococcus / Q000201:enzymology*; D014871:Water Microbiology
D008049:Lipase