A breakdown of Brassica self-incompatibility in ARC1 antisense transgenic plants.
Self-incompatibility, the rejection of self pollen, is the most widespread mechanism by which flowering plants prevent inbreeding. In Brassica, the S receptor kinase (SRK) has been implicated in the self-incompatibility response, but the molecular mechanisms involving SRK are unknown. One putative downstream effector for SRK is ARC1, a protein that binds to the SRK kinase domain. Here it is shown that suppression of ARC1 messenger RNA levels in the self-incompatible Brassica napus W1 line is correlated with a partial breakdown of self-incompatibility, resulting in seed production. This provides strong evidence that ARC1 is a positive effector of the Brassica self-incompatibility response.
Stone
S L
SL
Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
Arnoldo
M
M
Goring
D R
DR
D001937:Brassica / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology*; D016373:DNA, Antisense; D018525:Germination; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D018514:Plant Structures / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000502:physiology; D019076:Transgenes; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D016373:DNA, Antisense; D006023:Glycoproteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C064156:self-incompatibility locus specific glycoprotein, Brassica; D044767:Ubiquitin-Protein Ligases; D011494:Protein Kinases; C079832:S-receptor kinase