Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice.
Quantitative trait locus (QTL) mapping was used to identify chromosomal regions contributing to airway hyperresponsiveness in mice. Airway responsiveness to methacholine was measured in A/J and C3H/HeJ parental strains as well as in progeny derived from crosses between these strains. QTL mapping of backcross [(A/J x C3H/HeJ) x C3H/HeJ] progeny (n = 137-227 informative mice for markers tested) revealed two significant linkages to loci on chromosomes 6 and 7. The QTL on chromosome 6 confirms the previous report by others of a linkage in this region in the same genetic backgrounds; the second QTL, on chromosome 7, represents a novel locus. In addition, we obtained suggestive evidence for linkage (logarithm of odds ratio = 1.7) on chromosome 17, which lies in the same region previously identified in a cross between A/J and C57BL/6J mice. Airway responsiveness in a cross between A/J and C3H/HeJ mice is under the control of at least two major genetic loci, with evidence for a third locus that has been previously implicated in an A/J and C57BL/6J cross; this indicates that multiple genetic factors control the expression of this phenotype.
De Sanctis
G T
GT
Combined Program in Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston 02115, USA.
Singer
J B
JB
Jiao
A
A
Yandava
C N
CN
Lee
Y H
YH
Haynes
T C
TC
Lander
E S
ES
Beier
D R
DR
Drazen
J M
JM
D000818:Animals; D001249:Asthma / Q000235:genetics*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000235:genetics*; D001985:Bronchial Provocation Tests; D016085:Bronchoconstrictor Agents; D002874:Chromosome Mapping; D002875:Chromosomes; D008040:Genetic Linkage; D005838:Genotype; D008297:Male; D016210:Methacholine Chloride; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D019655:Quantitative Trait, Heritable
D016085:Bronchoconstrictor Agents; D016210:Methacholine Chloride