title: Capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus. Structural study of an undecasaccharide obtained by lithium degradation.
abstract: The capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus has been subjected to a specific degradation with lithium in ethylenediamine. The released undecasaccharide attached to one unit of tetrahydroxycyclopentanecarboxylic acid has been characterized by a combination of 2D nuclear magnetic resonance spectroscopy, mass spectrometry, monosaccharidic composition and linkage analyses. From the overlap of the structure of this oligosaccharide with previously identified di-, tri- and pentasaccharides released by mild acid hydrolysis, the capsular polysaccharide was deduced to have a pentadecasaccharide repeating unit with the following structure:
authors.affiliation: Equipe de Blycobiologie Végétale, Institut de Biotechnologie, Université de Limoges, France. vgloaguen@unilim.fr
authors.forename: V
authors.initials: V
authors.lastname: Gloaguen
authors.forename: H
authors.initials: H
authors.lastname: Morvan
authors.forename: L
authors.initials: L
authors.lastname: Hoffmann
authors.forename: Y
authors.initials: Y
authors.lastname: Plancke
authors.forename: J M
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: G
authors.initials: G
authors.lastname: Lippens
authors.forename: G
authors.initials: G
authors.lastname: Strecker
mesh_terms: D002240:Carbohydrate Sequence; D000458:Cyanobacteria / Q000737:chemistry*; D005029:Ethylenediamines; D008094:Lithium; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005029:Ethylenediamines; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C031234:ethylenediamine; D008094:Lithium