title: A translation repressor element resides in the 3' untranslated region of human p53 mRNA.
abstract: The 3' untranslated region of human p53 mRNA represses translation both in vitro and in vivo. Here, we identify a cis-acting 66-nucleotide U-rich sequence in the human p53 mRNA 3' untranslated region that mediates translational repression. Using UV cross-linking, we detect a 40 kDa protein that interacts specifically with the p53 3'UTR containing the repressor element. Enhanced translation of p53 mRNA contributes to the accumulation of p53 protein in cells exposed to gamma-radiation and could be a consequence of relieving the inhibition mediated by the repressor element.
authors.affiliation: Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Fu
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: S
authors.initials: S
authors.lastname: Benchimol
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D017931:DNA Primers; D005786:Gene Expression Regulation; D006801:Humans; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53