title: Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period.
abstract: RANTES may be one of the chemoattractants involved in stimulating eosinophils and macrophages to migrate selectively into bovine dominant follicles and into developing corpora lutea. We sequenced a 736 bp fragment of the bovine RANTES mRNA encoding the complete protein and defined the ovarian source of RANTES mRNA. As demonstrated by competitive RT-PCR, follicle-derived macrophages showed a 100-1000 times higher RANTES mRNA level compared to unpurified granulosa cells or follicle-derived fibroblasts. By means of in situ hybridization, RANTES mRNA positive macrophages were located in the former thecal layer of the developing corpora lutea.
authors.affiliation: Institute of Anatomy, University of Leipzig, Liebigstrasse 13, Leipzig, Germany. ausg@medizin.uni-leipzig.de
authors.forename: G
authors.initials: G
authors.lastname: Aust
authors.forename: E
authors.initials: E
authors.lastname: Brylla
authors.forename: I
authors.initials: I
authors.lastname: Lehmann
authors.forename: S
authors.initials: S
authors.lastname: Kiessling
authors.forename: K
authors.initials: K
authors.lastname: Spanel-Borowski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000096:biosynthesis* / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D017403:In Situ Hybridization; D008264:Macrophages / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism*; D010060:Ovulation / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D018946:Chemokine CCL5; D018076:DNA, Complementary; D012333:RNA, Messenger