The cloning, mapping and expression of a novel gene, BRL, related to the AF10 leukaemia gene.
The MLL gene is reciprocally translocated with one of a number of different partner genes in a proportion of human acute leukaemias. The precise mechanism of oncogenic transformation is unclear since most of the partner genes encode unrelated proteins. However, two partner genes, AF10 and AF17 are related through the presence of a cysteine rich region and a leucine zipper. The identification of other proteins with these structures will aid our understanding of their role in normal and leukaemic cells. We report the cloning of a novel human gene (BRL) which encodes a protein containing a cysteine rich region related to that of AF10 and AF17 and is overall most closely related to the previously known protein BR140. BRL maps to chromosome 22q13 and shows high levels of expression in testis and several cell lines. The deduced protein sequence also contains a bromodomain, four potential LXXLL motifs and four predicted nuclear localization signals. A monoclonal antibody raised to a BRL peptide sequence confirmed its widespread expression as a 120 Kd protein and demonstrated localization to the nucleus within spermatocytes.
McCullagh
P
P
Imperial Cancer Research Fund, Department of Medical Oncology, St Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London ECIM 6BQ, UK.
Chaplin
T
T
Meerabux
J
J
Grenzelias
D
D
Lillington
D
D
Poulsom
R
R
Gregorini
A
A
Saha
V
V
Young
B D
BD
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D051548:Histone Acetyltransferases; D056488:Histone Chaperones; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007938:Leukemia / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D017421:Sequence Analysis; D013347:Subcellular Fractions; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D000092468:Bromodomain Containing Proteins
D004268:DNA-Binding Proteins; D051548:Histone Acetyltransferases; D056488:Histone Chaperones; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C092513:MLLT10 protein, human; C096106:MLLT6 protein, human; C490477:Mllt10 protein, mouse; C486273:Mllt6 protein, mouse; C402649:BRD1 protein, human; D000092468:Bromodomain Containing Proteins