Cloning and characterization of iron-containing superoxide dismutase from the human malaria species Plasmodium ovale, P. malariae and P. vivax.
The iron-containing superoxide dismutase (FeSOD) gene from three human malaria species, namely Plasmodium ovale, P. malariae and P. vivax, was amplified by polymerase chain reaction, cloned and then sequenced. Comparisons of their deduced amino acid sequences with that of the FeSOD from P. falciparum revealed a very low polymorphism at the FeSOD locus in human malaria species. One P. ovale and the P. vivax FeSOD genes presented the same nucleotide sequence as that of the P. falciparum strain HB3 whereas the second P. ovale and the P. malariae genes exhibited two punctual mutations. These mutations did not affect the function and structure of the enzyme. The FeSOD polymorphism was so low that no phylogenetic relationship among human malaria species could be proposed, but this conservative structure strengthened the potentiality of this enzyme as a possible target for antimalarial drugs.
Baert
C B
CB
Centre International de Recherches Médicales de Franceville, Gabon.
Deloron
P
P
Viscogliosi
E
E
Delgado-Viscogliosi
P
P
Camus
D
D
Dive
D
D
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017125:Genes, Protozoan; D006801:Humans; D007501:Iron / Q000032:analysis; D008288:Malaria / Q000469:parasitology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010961:Plasmodium / Q000201:enzymology* / Q000235:genetics; D010965:Plasmodium malariae / Q000201:enzymology / Q000235:genetics; D010966:Plasmodium vivax / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D007501:Iron; D013482:Superoxide Dismutase