Evaluation of eukaryotic promoters for the construction of DNA vaccines for aquaculture.
We evaluated fish promoters as an alternative to viral promoters in the construction of DNA vaccines for aquaculture. A carp beta-actin promoter drove expression of the luciferase gene in live fish tissue to levels comparable to the CMVtk promoter.
Gómez-Chiarri
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Department of Fisheries, Animal and Veterinary Science, University of Rhode Island, Kingston 02881, USA. gomezchi@uri.edu
Chiaverini
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LA
D000199:Actins / Q000235:genetics; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002347:Carps / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D005393:Fish Diseases / Q000517:prevention & control; D005398:Fisheries / Q000379:methods; D017930:Genes, Reporter; D005814:Genes, Viral; D007114:Immunization / Q000662:veterinary*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D020317:Salmo salar / Q000235:genetics / Q000378:metabolism*; D013937:Thymidine Kinase / Q000235:genetics; D019444:Vaccines, DNA; D014714:Vertebrates / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D000199:Actins; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D008156:Luciferases; D013937:Thymidine Kinase