title: Three-dimensional structures of the TAFII-containing complexes TFIID and TFTC.
abstract: TBP (TATA-binding protein)-associated factors (TAF(II)s) are components of large multiprotein complexes such as TFIID, TFTC, STAGA, PCAF/GCN5, and SAGA, which play a key role in the regulation of gene expression by RNA polymerase II. The structures of TFIID and TFTC have been determined at 3.5-nanometer resolution by electron microscopy and digital image analysis of single particles. Human TFIID resembles a macromolecular clamp that contains four globular domains organized around a solvent-accessible groove of a size suitable to bind DNA. TFTC is larger and contains five domains, four of which are similar to TFIID.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/Université Louis Pasteur, Boite Postale 163, F-67404 Illkirch cedex, Communauté Urbaine de Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Brand
authors.forename: C
authors.initials: C
authors.lastname: Leurent
authors.forename: V
authors.initials: V
authors.lastname: Mallouh
authors.forename: L
authors.initials: L
authors.lastname: Tora
authors.forename: P
authors.initials: P
authors.lastname: Schultz
mesh_terms: D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry*; D019473:Transcription Factors, TFII / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; C089768:TAF10 protein, human; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII