Telomeric repeat organization--a comparative in situ study between man and rodent.
Human, hamster, and mouse chromosomes show both similarities and differences in telomeric organization, detectable with a novel version of the PRINS technique. The differences allowed us to investigate the fate of the telomeres on a chromosome from one species when this chromosome was introduced into the cells of another species. For this purpose, we tested telomeres in cell lines of somatic cell hybrids containing human chromosomes on a rodent background, finding that the telomeres on human chromosomes could not be discriminated from the telomeres on rodent chromosomes. All telomeres in the cell lines were much shorter than the telomeres in normal cells. In the mouse-derived cell lines, half of the mouse chromosomes were fused to other mouse chromosomes at the ends of their short arms. At the points of fusion we were generally unable to detect telomeric signals. In these cell lines, we also found a fraction of chromosomes ends with only one telomeric signal. In chromosomes where both ends showed only one signal, the relative orientation of the signals appeared to be nonrandom with respect to sister chromatids.
Serakinci
N
N
Department of Cytogenetics, The Danish Cancer Society, Aarhus, Denmark.
Krejcí
K
K
Koch
J
J
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D006224:Cricetinae; D006801:Humans; D007621:Karyotyping; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D016615:Telomere / Q000235:genetics* / Q000648:ultrastructure