Nitric oxide is required for the maintenance but not initiation of ganglionic long-term potentiation.
The role of nitric oxide in long-term potentiation of the nicotinic pathway of synaptic transmission in the isolated superior cervical ganglia of rat was studied. Long-term potentiation was induced by a brief tetanizing pulse (tetanus, 20 Hz/20 s) to the preganglionic nerve. The amplitude of the extracellularly recorded postganglionic compound action potential was used as an index of synaptic transmission. Pretreatment with the nitric oxide synthase inhibitor N(G)-nitro-L-arginine methyl ester (10 microM) or L-N(G)-nitro-arginine (10 microM) 30 min before tetanus, inhibited long-term potentiation. The inactive enantiomer of the nitric oxide synthase inhibitor, N(G)-nitro-D-arginine methyl ester (10 microM), failed to inhibit the long-term potentiation when given 30 min before the tetanus. Washout of L-N(G)-nitro-arginine, but not N(G)-nitro-L-arginine methyl ester, resulted in complete recovery of long-term potentiation. The nitric oxide synthase inhibitor had no significant effect on the basal ganglionic neurotransmission or post-tetanic potentiation. Furthermore, established long-term potentiation was blocked by superfusion of ganglia with N(G)-nitro-L-arginine methyl ester 1 h after a tetanus. Pretreatment of ganglia with the nitric oxide donor, sodium nitroprusside (100 microM), or the nitric oxide synthase substrate, L-arginine (1 mM), completely prevented the inhibitory effects of N(G)-nitro-L-arginine methyl ester on the tetanus-induced long-term potentiation. These findings present evidence for a requirement of nitric oxide for the maintenance but not induction of long-term potentiation in rat isolated superior cervical ganglia.
Altememi
G F
GF
Department of Pharmacology, College of Pharmacy, University of Houston, TX 77204-5515, USA.
Alkadhi
K A
KA
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004558:Electric Stimulation; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology*; D019335:Nitroarginine / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D009599:Nitroprusside; D019335:Nitroarginine; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester