Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region.
Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. We molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the aorta-gonad-mesonephros (AGM) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+ CD45 cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1 +CD45-cells were injected into neonatal liver of busulfan-treated mice. Thus, PCLP1 can be used to separate hemangioblasts that give rise to LTR-HSCs.
Hara
T
T
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan. thara@rinshoken.or.jp
Nakano
Y
Y
Tanaka
M
M
Tamura
K
K
Sekiguchi
T
T
Minehata
K
K
Copeland
N G
NG
Jenkins
N A
NA
Okabe
M
M
Kogo
H
H
Mukouyama
Y
Y
Miyajima
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000196:embryology*; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D002874:Chromosome Mapping; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology*; D005326:Fetal Proteins / Q000032:analysis*; D005865:Gestational Age; D006066:Gonads / Q000737:chemistry / Q000196:embryology*; D006133:Growth Substances / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D017493:Leukocyte Common Antigens / Q000032:analysis; D008077:Lipoproteins, LDL / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D008650:Mesonephros / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D053683:Oncostatin M; D010455:Peptides / Q000494:pharmacology / Q000502:physiology*; D013234:Stem Cells / Q000737:chemistry* / Q000145:classification / Q000187:drug effects
D015415:Biomarkers; D005326:Fetal Proteins; D006133:Growth Substances; D008077:Lipoproteins, LDL; D008562:Membrane Glycoproteins; C508352:Osm protein, mouse; D010455:Peptides; C032629:acetyl-LDL; C097007:podocalyxin-like protein 1, Oryctolagus cuniculus; D053683:Oncostatin M; D017493:Leukocyte Common Antigens