Cloning of the Dam methyltransferase gene from Haemophilus influenzae bacteriophage HP1.
The putative product of orf13 from the genome of Haemophilus influenzae HP1 bacteriophage shows homology only to bacteriophage T1 Dam methyltransferase, and a weak similarity to the conserved amino acids sequence motifs characteristic of m6A-methyltransferases. Especially interesting is lack of characteristic motif I responsible for binding of S-adenosylmethionine. Despite this fact, a DNA sequence of HP1 bacteriophage of Haemophilus influenzae encoding methyltransferase activity was cloned and expressed in Escherichia coli using pMPMT4 omega expression vector. The cloned methyltransferase recognizes the sequence 5'-GATC-3' and methylates an adenine residue. The enzyme methylates both double- and single-stranded DNA substrates.
Piekarowicz
A
A
Institute of Microbiology, Warsaw University, Poland.
Bujnicki
J
J
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D005814:Genes, Viral; D005822:Genetic Vectors; D006193:Haemophilus influenzae / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012436:S-Adenosylmethionine / Q000378:metabolism; D016415:Sequence Alignment; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics*
D012436:S-Adenosylmethionine; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)