Analysis of repair of abasic sites in early onset breast cancer patients.
Defective DNA repair has been suggested as a possible predisposing factor for breast cancer. We have investigated the repair of the frequent endogenous lesions abasic sites in sporadic early onset breast cancer patients and matched control individuals. No significant difference was observed between the abasic site repair capacities of peripheral blood lymphocytes from cases and controls. Repair of abasic sites was also studied in tumor and surrounding normal tissues of the patients. The 2 tissues showed marked differences in histology and protein composition with a fibro-collagenous component varying from sample to sample but invariably higher in normal tissues as compared with the adjacent tumor. These differences involved the need to calculate the repair activities of tissues on the basis of cellular DNA content for comparison purposes. After doing so, tumor and normal tissues exhibited similar abasic site repair capacities, whereas lymphocytes showed a repair capacity significantly lower than tissues. We conclude that early onset sporadic breast cancer patients show no evident defect in repair of abasic sites at the level of both lymphocytes and tumor.
Rossi
O
O
DNA Repair Unit, Mutagenesis Laboratory, Istituto Nazionale Ricerca Cancro, Genoa, Italy.
Carrozzino
F
F
Cappelli
E
E
Carli
F
F
Frosina
G
G
D000328:Adult; D017668:Age of Onset; D001665:Binding Sites / Q000235:genetics; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D019757:Carbon-Oxygen Lyases / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004260:DNA Repair / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics; D004273:DNA, Neoplasm / Q000378:metabolism*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D005260:Female; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D008875:Middle Aged; D010957:Plasmids / Q000378:metabolism; D014020:Tissue Extracts / Q000378:metabolism
D004273:DNA, Neoplasm; D014020:Tissue Extracts; D043223:Deoxyribonuclease IV (Phage T4-Induced); D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase