title: Conserved characteristics of heterochromatin-forming DNA at the 15q11-q13 imprinting center.
abstract: Nuclear matrix binding assays (NMBAs) define certain DNA sequences as matrix attachment regions (MARs), which often have cis-acting epigenetic regulatory functions. We used NMBAs to analyze the functionally important 15q11-q13 imprinting center (IC). We find that the IC is composed of an unusually high density of MARs, located in close proximity to the germ line elements that are proposed to direct imprint switching in this region. Moreover, we find that the organization of MARs is the same at the homologous mouse locus, despite extensive divergence of DNA sequence. MARs of this size are not usually associated with genes but rather with heterochromatin-forming areas of the genome. In contrast, the 15q11-q13 region contains multiple transcribed genes and is unusual for being subject to genomic imprinting, causing the maternal chromosome to be more transcriptionally silent, methylated, and late replicating than the paternal chromosome. We suggest that the extensive MAR sequences at the IC are organized as heterochromatin during oogenesis, an organization disrupted during spermatogenesis. Consistent with this model, multicolor fluorescence in situ hybridization to halo nuclei demonstrates a strong matrix association of the maternal IC, whereas the paternal IC is more decondensed, extending into the nuclear halo. This model also provides a mechanism for spreading of the imprinting signal, because heterochromatin at the IC on the maternal chromosome may exert a suppressive position effect in cis. We propose that the germ line elements at the 15q11-q13 IC mediate their effects through the candidate heterochromatin-forming DNA identified in this study.
authors.affiliation: Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA. john.greally@yale.edu
authors.forename: J M
authors.initials: JM
authors.lastname: Greally
authors.forename: T A
authors.initials: TA
authors.lastname: Gray
authors.forename: J M
authors.initials: JM
authors.lastname: Gabriel
authors.forename: L
authors.initials: L
authors.lastname: Song
authors.forename: S
authors.initials: S
authors.lastname: Zemel
authors.forename: R D
authors.initials: RD
authors.lastname: Nicholls
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D002884:Chromosomes, Human, Pair 15; D004247:DNA / Q000737:chemistry*; D005260:Female; D018392:Genomic Imprinting; D006570:Heterochromatin; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
chemical_list: D006570:Heterochromatin; D012261:Ribonucleoproteins; D004247:DNA; C119425:MKRN3 protein, human; C119432:Mkrn3 protein, mouse; D044767:Ubiquitin-Protein Ligases