Chromosomal aberrations in humans as genetic endpoints to assess the impact of pollution.
The chromosomal aberration assay with peripheral blood lymphocytes has been used routinely during the last three decades to survey exposure of humans to various genotoxic agents. A large number of biomonitoring studies are based on this genetic endpoint. A great deal of data exists on occupational, life-style or medical exposure situations but less evidence of the validity of the assay is available with regards to environmental exposure. In the present paper we report our investigations on the impact of pollution in two different populations using chromosomal aberrations in human peripheral blood lymphocytes as a biomarker of chronic exposure to heavy metals and dioxins/furans for a long period and as a biomarker of acute exposure to accidentally released vinyl chloride in the air. In order to study genotoxic effects (chromosomal aberrations) of heavy metals and dioxins/furans, 52 exposed individuals from a polluted area were compared to 51 matched controls from a distant non-industrialized area. A statistically significant increase was observed in the frequency of chromosomal aberrations in peripheral blood lymphocytes from the exposed population (1.90% aberrant cells vs. 1.11% for the controls). In the case of the vinyl chloride accident, chromosomal aberrations were analyzed in peripheral blood lymphocytes from 29 potentially exposed and 29 non-exposed individuals (matched controls). The exposed group showed a statistically significant increase in the frequency of aberrant cells (1.47% vs. 1.07% for the controls).
Hüttner
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Institute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, Germany. huettner@ipk-gatersleben.de
Götze
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Nikolova
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D000062:Accidents, Occupational; D000328:Adult; D000393:Air Pollutants / Q000009:adverse effects*; D015415:Biomarkers; D002452:Cell Count; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000187:drug effects*; D004249:DNA Damage; D004147:Dioxins / Q000009:adverse effects; D004781:Environmental Exposure / Q000009:adverse effects*; D004784:Environmental Monitoring; D005260:Female; D005663:Furans / Q000009:adverse effects; D005858:Germany; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D019216:Metals, Heavy / Q000009:adverse effects; D008875:Middle Aged; D014752:Vinyl Chloride / Q000506:poisoning
D000393:Air Pollutants; D015415:Biomarkers; D004147:Dioxins; D005663:Furans; D019216:Metals, Heavy; D014752:Vinyl Chloride