A nine-codon deletion mutation in the cytomegalovirus UL97 phosphotransferase gene confers resistance to ganciclovir.
A deletion mutation (codons 595 to 603) in the cytomegalovirus (CMV) UL97 gene was recently reported after sequence analysis of leukocyte DNA from a patient receiving ganciclovir. The corresponding viral phenotype was examined by transfer of this mutation to a laboratory CMV strain (strain Towne). The recombinant virus was resistant to ganciclovir (8.4-fold increase in the 50% inhibitory concentration), was sensitive to foscarnet, and replicated normally in cell culture.
Chou
S
S
Medical and Research Services, Veterans Affairs Medical Center, Portland, Oregon 97201, USA. chous@ohsu.edu
Meichsner
C L
CL
D000998:Antiviral Agents / Q000494:pharmacology*; D003062:Codon; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics; D004352:Drug Resistance, Microbial; D015774:Ganciclovir / Q000494:pharmacology*; D017353:Gene Deletion; D005814:Genes, Viral; D006801:Humans; D009154:Mutation; D010770:Phosphotransferases / Q000235:genetics*
D000998:Antiviral Agents; D003062:Codon; D010770:Phosphotransferases; D015774:Ganciclovir