Identification of differentially expressed genes in Con A-activated carp (Cyprinus carpio L.) leucocytes.
A cDNA library was constructed from the message RNA (mRNA) obtained from Con A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Differential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induced HK leucocytes of carp. The differential expression patterns of certain cDNA clones were confirmed by Southern-blot and Northern-blot analysis. Single-pass of the sequencing analysis and homology search in Genbank (EMBL) revealed those differentially expressed cDNA clones encode for cytochrome c oxidase sub-unit II and III (COII and COIII), elongation factor-1 beta (EF-1 beta), bleomycin hydrolase (BH), heat shock cognate protein 70 (HSC70) and 16S ribosomal RNA (16S rRNA).
Yin
Z
Z
Department of Biological Sciences, National University of Singapore, Singapore.
He
J Y
JY
Gong
Z
Z
Lam
T J
TJ
Sin
Y M
YM
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002347:Carps / Q000235:genetics* / Q000276:immunology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D003208:Concanavalin A / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology; D004274:DNA, Recombinant; D003576:Electron Transport Complex IV / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D005796:Genes / Q000235:genetics*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000235:genetics; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000276:immunology; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D004274:DNA, Recombinant; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D020648:Peptide Elongation Factor 1; D012336:RNA, Ribosomal, 16S; D003208:Concanavalin A; D003576:Electron Transport Complex IV; D003546:Cysteine Endopeptidases