title: Heterogeneity of anti-dsDNA antibodies in their cross-reaction with ribosomal P protein.
abstract: We have investigated the possible cross-reaction of anti-dsDNA antibodies with ribosomal P peptide for several reasons. First, the antibodies frequently occur together, and secondly, they vary similarly with disease activity. Human polyclonal anti-dsDNA antibodies were affinity purified from eight patients and anti-ribosomal P antibodies from two patients with systemic Lupus erythematosus (SLE) who had high titers of anti-dsDNA as well as anti-ribosomal P antibodies. Nine of the 10 sera were totally specific in their reactivity with their cognate antigens. In only one patient did we find a subpopulation of antibodies which cross-reacted with both dsDNA and the carboxyl terminal 22 amino acid peptide. Our results indicate that anti-dsDNA antibodies are heterogeneous and usually do not cross-react with the carboxyl terminal P peptide, but on occasion (1/10) a patient will produce anti-dsDNA antibodies cross-reactive with the carboxyl terminal P peptide.
authors.affiliation: Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
authors.forename: I
authors.initials: I
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Rayno
authors.forename: M
authors.initials: M
authors.lastname: Wolfson-Reichlin
authors.forename: M
authors.initials: M
authors.lastname: Reichlin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000915:Antibody Affinity; D002417:Cattle; D003429:Cross Reactions; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D012269:Ribosomal Proteins / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; C064601:L12E protein, Trypanosoma cruzi; D015800:Protozoan Proteins; D012269:Ribosomal Proteins