A simple acute in vivo comparative test for sensitivity to insulin in the mouse.
A method is described for measuring the acute blood glucose response to an insulin challenge which requires only 6 samples of 20 microl of blood collected over a 4 hour period. This evaluation of sensitivity to insulin was validated by comparing the effects of gliclazide, metformin and a novel antidiabetic imidazoline compound (S22068) on the blood glucose response. The test distinguished between the insulin-secreting and hypoglycaemic action of gliclazide and the insulin-sensitizing actions of metformin and S22068. The test has the advantage that it can be repeated in the same animal after a period of recovery and thus enables the overall sensitivity to insulin to be compared before and after acute or chronic dose regimes.
Williams
C A
CA
Department of Pharmacology, School of Medical Sciences, University of Bristol, UK.
Shih
M F
MF
Taberner
P V
PV
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005907:Gliclazide / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000097:blood* / Q000494:pharmacology*; D008297:Male; D008687:Metformin / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D011934:Reagent Strips
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D011934:Reagent Strips; D008687:Metformin; D005907:Gliclazide