title: Distribution of IS900 restriction fragment length polymorphism types among animal Mycobacterium avium subsp. paratuberculosis isolates from Argentina and Europe.
abstract: Sixty-one Mycobacterium avium subsp. paratuberculosis isolates from cattle and deer from the Buenos Aires province, an important livestock region in Argentina, were typed by restriction fragment length polymorphisms (RFLP) analysis based on IS900. Four different RFLP patterns (designated 'A', 'B', 'C' and 'E') were identified in BstEII digests of genomic DNA. The most frequently observed type, pattern 'A', was found in 46 isolates (75%). The second, pattern 'E', included 8 isolates (13%), while the third, pattern 'B', included 6 isolates (10%). Pattern 'C' was found for only one isolate. All of the deer isolates were classified as pattern 'A', while cattle isolates represented all four RFLP patterns. Twenty-one isolates representing the four different BstEII-RFLP patterns were digested with PstI. Twenty isolates showed identical PstI-RFLP pattern. BstEII-RFLP patterns from Argentine cattle and deer were compared with patterns found in cattle, goat, deer, rabbit, and human isolates from Europe. The most common pattern in Argentina, pattern 'A', was identical to a less frequently occurring pattern R9 (C17) from Europe. The other Argentine patterns 'B', 'C' and 'E', were not found in the Europe. These results indicate that the distribution of M. avium subsp. paratuberculosis genotypes in the Buenos Aires province of Argentina is different from that found in Europe.
authors.affiliation: Grupo de Sanidad Animal EEA-INTA Balcarcea, Buenos Aires, Argentina.
authors.forename: A R
authors.initials: AR
authors.lastname: Moreira
authors.forename: F
authors.initials: F
authors.lastname: Paolicchi
authors.forename: C
authors.initials: C
authors.lastname: Morsella
authors.forename: M
authors.initials: M
authors.lastname: Zumarraga
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
authors.forename: B
authors.initials: B
authors.lastname: Fabiana
authors.forename: A
authors.initials: A
authors.lastname: Alicia
authors.forename: O
authors.initials: O
authors.lastname: Piet
authors.forename: D
authors.initials: D
authors.lastname: van Soolingen
authors.forename: R M
authors.initials: RM
authors.lastname: Isabel
mesh_terms: D000818:Animals; D001118:Argentina; D002417:Cattle; D003670:Deer; D005060:Europe; D006801:Humans; D009162:Mycobacterium avium / Q000235:genetics / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D011817:Rabbits; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*