Systematic analysis of S. cerevisiae chromosome VIII genes.
To begin genome-wide functional analysis, we analysed the consequences of deleting each of the 265 genes of chromosome VIII of Saccharomyces cerevisiae. For 33% of the deletion strains a growth phenotype could be detected: 18% of the genes are essential for growth on complete glucose medium, and 15% grow significantly more slowly than the wild-type strain or exhibit a conditional phenotype when incubated under one of 20 different growth conditions. Two-thirds of the mutants that exhibit conditional phenotypes are pleiotropic; about one-third of the mutants exhibit only one phenotype. We also measured the level of expression directed by the promoter of each gene. About half of the promoters direct detectable transcription in rich glucose medium, and most of these exhibited only low or medium activity. Only 1% of the genes are expressed at about the same level as ACT1. The number of active promoters increased to 76% upon growth on a non-fermentable carbon source, and to 93% in minimal glucose medium. The majority of promoters fluctuated in strength, depending on the medium.
Niedenthal
R
R
Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, Geb. 26.12.01.64, 40225 Düsseldorf, Germany.
Riles
L
L
Güldener
U
U
Klein
S
S
Johnston
M
M
Hegemann
J H
JH
D017931:DNA Primers / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry; D005434:Flow Cytometry; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006836:Hydro-Lyases; D007202:Indicators and Reagents / Q000737:chemistry; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D010641:Phenotype; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D004271:DNA, Fungal; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase; D005947:Glucose