Expression of tyrosinase, TRP-1 and TRP-2 in ultraviolet-irradiated human melanomas and melanocytes: TRP-2 protects melanoma cells from ultraviolet B induced apoptosis.
Tyrosinase related protein (TRP)-1 and TRP-2 are known to regulate the quality of melanin, and recently their potential role in inhibiting apoptosis have also been reported. To study the role of tyrosinase, TRP-1 and TRP-2 in the growth, differentiation and cell death of ultraviolet B (UVB) irradiated melanocytes, the expression of these proteins in amelanotic and melanotic cells was examined. Expression of tyrosinase and TRP-1 correlated with melanin content, which was upregulated after repeated irradiation of melanotic cells by low doses of UVB. In contrast, the expression and activity of TRP-2 correlated with cell proliferation, but not with pigmentation. In one melanotic melanoma cell line, significant suppression of cell proliferation was observed after low or high doses of UVB irradiation, possibly due to apoptotic changes. TRP-2 expression was remarkably reduced in UVB-irradiated cells, and transfection with TRP-2 expression vector rescued these cells from UVB-induced apoptosis. These results indicate that TRP-2 expression is closely associated with the regulation of cell growth/survival of melanocytes exposed to UVB and that TRP-2 plays a role in protecting melanoma cells from UVB-induced apoptosis.
Nishioka
E
E
Department of Dermatology, Kobe University School of Medicine, Japan.
Funasaka
Y
Y
Kondoh
H
H
Chakraborty
A K
AK
Mishima
Y
Y
Ichihashi
M
M
D000328:Adult; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D017209:Apoptosis / Q000235:genetics; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000502:physiology; D008297:Male; D008543:Melanins / Q000096:biosynthesis / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology / Q000528:radiation effects*; D008545:Melanoma / Q000201:enzymology* / Q000473:pathology / Q000532:radiotherapy; D008562:Membrane Glycoproteins; D014442:Monophenol Monooxygenase / Q000096:biosynthesis*; D010088:Oxidoreductases; D014176:Protein Biosynthesis; D014466:Ultraviolet Rays
D000951:Antigens, Neoplasm; D008543:Melanins; D008562:Membrane Glycoproteins; C016042:phaeomelanin; C041877:eumelanin; D010088:Oxidoreductases; C489799:TYRP1 protein, human; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase