Free thiol groups are essential for infectivity of human cytomegalovirus.
The membrane-impermeable thiol blocker 5'5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.
Mirazimi
Ali
A
Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
Mousavi-Jazi
Mehrdad
M
Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
Sundqvist
Vivi-Anne
VA
Department of Immunology, Microbiology, Pathology and Infectious Disease, Karolinska Institute, Box 12 773, 112 96 Stockholm, Sweden 2.
Svensson
Lennart
L
Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
D003587:Cytomegalovirus / Q000187:drug effects / Q000472:pathogenicity*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006801:Humans; D013438:Sulfhydryl Compounds / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000502:physiology
D013438:Sulfhydryl Compounds; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D004228:Dithionitrobenzoic Acid; D004229:Dithiothreitol