Analysis of porcine cytomegalovirus DNA polymerase by consensus primer PCR.
We used a consensus primer PCR method to amplify a region of herpesviral DNA-directed DNA polymerase gene using degenerate primers for initial characterization of the porcine cytomegalovirus (PCMV) genome. The sequence of the PCR product from PCMV DNA template and its alignment with other herpesvirus DNA polymerase counterparts showed that both conserved amino acid residues and conservative amino acid substitutions are in parallel. Phylogenetic analysis revealed that PCMV should be included in the clade comprising human herpesvirus 6 and 7, rather than human and mouse cytomegaloviruses, in Betaherpesvirus subfamily.
Rupasinghe
V
V
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Tajima
T
T
Maeda
K
K
Iwatsuki-Horimoto
K
K
Sugii
S
S
Horimoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D017124:Conserved Sequence; D003587:Cytomegalovirus / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D017931:DNA Primers; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase