Assay of 2-naphthol in human urine by high-performance liquid chromatography.
This paper describes a novel liquid chromatographic method for the quantitation of 2-naphthol in human urine. Urine samples were extracted after enzymatic hydrolysis of glucuronides and sulfates; 2-naphthol was then separated using reversed-phase high-performance liquid chromatography. The corresponding detection limits were 0.04 ng/ml for the standard sample in acetonitrile and 0.13 ng/ml for urine samples. The level of urinary 2-naphthol in 100 Korean shipyard workers was analyzed using this new method. The level ranged from 0.21 ng/ml (0.26 micromol/mol creatinine) to 34.19 ng/ml (59.11 micromol/mol creatinine), and the mean+/-standard deviation was 5.08 ng/ml (6.60 micromol/mol creatinine)+/-5.75 ng/ml (9.22 micromol/mol creatinine). The mean+/-standard deviation of urinary 2-naphthol level of smokers, 7.03 ng/ml (8.49 micromol/mol creatinine)+/-6.16 ng/ml (10.23 micromol/mol creatinine), was significantly higher than that of non-smokers, 2.49 ng/ml (4.10 micromol/mol creatinine)+/-3.92 ng/ml (7.03 micromol/mol creatinine).
Kim
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Department of Preventive Medicine, College of Medicine, Chungbuk National University, Cheongju, South Korea. kimheon@med.chungbuk.ac.kr
Kim
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YD
Lee
H
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Kawamoto
T
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Yang
M
M
Katoh
T
T
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005966:Glucuronidase / Q000378:metabolism; D020719:Glucuronides / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007221:Industry; D007723:Korea; D009284:Naphthols / Q000652:urine*; D016273:Occupational Exposure; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012767:Ships; D013050:Spectrometry, Fluorescence; D013431:Sulfates / Q000652:urine
D000097:Acetonitriles; D020719:Glucuronides; D009284:Naphthols; D013431:Sulfates; D005966:Glucuronidase; C028405:2-naphthol; C032159:acetonitrile