title: Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts.
abstract: Asymmetric cell division generates daughter cells with different developmental fates from progenitor cells that contain localized determinants. During this division, the asymmetric localization of cell-fate determinants and the orientation of the mitotic spindle must be precisely coordinated. In Drosophila neuroblasts, inscuteable controls both spindle orientation and the asymmetric localization of the cell-fate determinants Prospero and Numb. Inscuteable itself is localized in an apical cortical crescent and thus reflects the intrinsic asymmetry of the neuroblast. Here we show that localization of Inscuteable depends on Bazooka, a protein containing three PDZ domains with overall sequence similarity to Par-3 of Caenorhabditis elegans. Bazooka and Inscuteable form a complex that also contains Staufen, a protein responsible for the asymmetric localization of prospero messenger RNA. We propose that, after delamination of the neuroblast from the neuroepithelium, Bazooka provides an asymmetric cue in the apical cytocortex that is required to anchor Inscuteable. As Bazooka is also responsible for the maintenance of apical-basal polarity in epithelial tissues, it may be the missing link between epithelial polarity and neuroblast polarity.
authors.affiliation: Institut für Genetik, Heinrich-Heine-Universität Düsseldorf, Germany. wodarz@uni-duesseldorf.de
authors.forename: A
authors.initials: A
authors.lastname: Wodarz
authors.forename: A
authors.initials: A
authors.lastname: Ramrath
authors.forename: U
authors.initials: U
authors.lastname: Kuchinke
authors.forename: E
authors.initials: E
authors.lastname: Knust
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000502:physiology*; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D029721:Drosophila Proteins; D019476:Insect Proteins / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000502:physiology; D009687:Nuclear Proteins / Q000502:physiology; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000502:physiology; D014157:Transcription Factors
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C400774:baz protein, Drosophila; C098678:insc protein, Drosophila; C073099:pros protein, Drosophila; C069927:stau protein, Drosophila