title: Mast cell heterogeneity in the human uvea.
abstract: To identify chymase- and tryptase-positive mast cells in the human uvea, and to study their associations with different types of resident uveal cells, uveal specimens from 24 human donor eyes were cryosectioned in sagittal and tangential planes. Enzyme histochemical staining of chymase was combined with immunohistochemical staining for tryptase, detected with the APAAP method. Fluorescence immunohistochemistry was performed with antibodies against c-kit, alpha smooth muscle actin, protein gene product (PGP) 9.5, CD45, and HLA-DR. In different uveal compartments, the total amounts of mast cells were calculated and the distributions of chymase and tryptase were quantified. All uveal mast cells were c-kit and CD45 positive and HLA-DR negative. No association existed between mast cells and actin-containing cells. Only a few mast cells were in close association with PGP 9.5-labeled nerve fibers. In the choroid, most mast cells were located in the inner central part (mean density = 48.9/mm(2)), and contained both chymase and tryptase (96%). The ciliary muscle contained numerous mast cells (mean density = 33.7/mm(2)), many of them tryptase positive but chymase negative (63%). In the pars plana, a high number of chymase-positive, tryptase-negative mast cells were found (20%). In the iris only a few mast cells were present. Although the choroid contains the most common subtype of mast cells, a unique situation concerning the distribution of chymase and tryptase is present in the anterior uveal tissues. A possible role for these cells in the special immunological situation of the anterior eye chamber merits further investigation.
authors.affiliation: Anatomisches Institut II, Universität Erlangen-Nürnberg, Universitätsstrasse 19, D-91054 Erlangen, Germany. atmay@anatomie.uni-erlangen.de
authors.forename: C A
authors.initials: CA
authors.lastname: May
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002829:Choroid / Q000166:cytology / Q000201:enzymology; D053818:Chymases; D002924:Ciliary Body / Q000166:cytology / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D008407:Mast Cells / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D008875:Middle Aged; D012697:Serine Endopeptidases / Q000378:metabolism; D016707:Tissue Fixation; D053802:Tryptases; D014602:Uvea / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D000199:Actins; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases