Stable recombinant expression of the anti HIV-1 monoclonal antibody 2F5 after IgG3/IgG1 subclass switch in CHO cells.
The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain variable region to an IgG1 constant region and expressed as IgG1 kappa in CHO cells. CHO clones have been established, which stably express humAb 2F5 at high levels. The specificity, affinity and in vitro function of both isotypes were identical.
Kunert
R
R
Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, Haus B, A-1190 Vienna, Austria. R.Kunert@iam.boku.ac.at
Steinfellner
W
W
Purtscher
M
M
Assadian
A
A
Katinger
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000378:metabolism; D000915:Antibody Affinity; D000918:Antibody Specificity; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D000939:Epitopes / Q000378:metabolism; D015870:Gene Expression; D015483:HIV Antibodies / Q000235:genetics* / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D000939:Epitopes; D015483:HIV Antibodies; D007074:Immunoglobulin G