title: Morphological changes of the endolymphatic sac induced by microinjection of artificial endolymph into the cochlea.
abstract: Morphological changes of the endolymphatic sac were analyzed in guinea pigs following microinjection of artificial endolymph into the cochlea or withdrawal of a quantity of native endolymph. Injections were performed into the second turn of scala media with a micro-pump at a rate of 60-100 nl/min, lasting for a period of 4, 7. 5, 15 or 18 min. In withdrawal experiments, endolymph was aspirated from the second cochlear turn over a period of 8 min. For each procedure the contralateral (non-treated) ear served as a histological control. Following artificial endolymph injections of 7. 5 min or more there was an almost total absence of the normal intraluminal homogeneous substance (HS) on the injected side. Our observations suggest that the disappearance of the HS occurs by both enzymatic and macrophagic activity. After endolymphatic withdrawals the ES was found to contain increased amounts of HS. The results could suggest that the volume of fluid in the ES, and hence the volume of the entire membranous labyrinth, may be regulated by a dynamic relationship between active secretion and enzymatic degradation of a lumen-expanding substance that is intimately related to the intraluminal macrophages. The exact mechanism governing these regulatory systems, and their relationship to ion and water movements across the epithelium of the sac, remain to be elucidated.
authors.affiliation: Department of Otolaryngology, University Hospital (Akademiska sjukhuset) of Uppsala, 751 85 Uppsala, Sweden. helge.rask.andersen@ent.uas.lul.se
authors.forename: H
authors.initials: H
authors.lastname: Rask-Andersen
authors.forename: J E
authors.initials: JE
authors.lastname: DeMott
authors.forename: D
authors.initials: D
authors.lastname: Bagger-Sjöbäck
authors.forename: A N
authors.initials: AN
authors.lastname: Salt
mesh_terms: D000818:Animals; D003051:Cochlea / Q000187:drug effects* / Q000502:physiology; D004594:Electrophysiology; D004710:Endolymph / Q000737:chemistry / Q000502:physiology*; D004711:Endolymphatic Duct / Q000648:ultrastructure; D004712:Endolymphatic Sac / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure*; D006168:Guinea Pigs; D008845:Microinjections; D008854:Microscopy, Electron