The antigen 85 complex vaccine against experimental Mycobacterium leprae infection in mice.
The proteins in culture filtrate derived from Bacillus Calmette-Guérin (BCG) were examined for protection against infection by Mycobacterium leprae. Immunization with the major secreted proteins, antigen 85 complex (Ag 85) A, B and C, induced effective protective immunity against multiplication of M. leprae in the foot pads of mice. The most effective protection was observed when mice were immunized with Ag 85A. A single immunization with Ag 85 could induce antigen-specific interferon gamma (IFNgamma) synthesis and more effective protection than live BCG vaccine. This study demonstrates that Ag 85 is an important immunoprotective molecule against leprosy infection.
Naito
M
M
Department of Oral Bacteriology, Nagasaki University School of Dentistry, Sakamoto 1-7-1, Nagasaki, Japan.
Matsuoka
M
M
Ohara
N
N
Nomaguchi
H
H
Yamada
T
T
D000818:Animals; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007371:Interferon-gamma / Q000096:biosynthesis; D007918:Leprosy / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009166:Mycobacterium leprae / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; C065036:antigen 85, Mycobacterium bovis; D007371:Interferon-gamma