Temperature and hemodynamic changes associated with increased neural damage to global hemispheric hypoxic ischemia by prior prostaglandin E2, D2 and F2alpha administration.
Experiments compared the hemispheric neural damage resulting from global hemispheric hypoxic ischemia (GHHI, ligation of right common carotid artery plus 35 min of 12% O2) in groups of anesthetized, male Long Evans rats, 9-10 weeks of age, kept at 37 degrees C, and previously given an intracerebroventricular (i.c.v., 2.5 microl) injection of 28 or 70 pmoles of PGE2, PGF2alpha or PGD2 or sterile saline (SS) 30 min beforehand. Mean arterial pressure (MAP), ipsilateral cortical capillary blood flow (CBF), colonic (Tc), ipsilateral (Tipsi) and contralateral (Tcontra), temporalis muscle temperatures were measured before, during and for 15 min after GHHI. Necrotic neural damage was assessed 7 days post-GHHI. All groups given GHHI + PGs showed increased ipsilateral hemispheric damage to GHHI especially due to enhanced neocortical damage, compared to the saline control group given the same insult. PGD2 was the most potent PG to cause further damage to the global insult. Tc, Tipsi, Tcontra and MAP increased following the i.c.v. injection of PGE2. I.c.v. PGF2alpha transiently decreased MAP, PGD2 tended to decrease cerebral blood flow and neither evoked changes in temperature compared to respective pre-injection control values. Results demonstrate increased neural damage to GHHI with prior i.c.v. PGE2, PGF2alpha or PGD2 administration.
Thornhill
J
J
Department of Physiology and Saskatchewan Stroke Research Center, University of Saskatchewan, Saskatoon, Canada. thornhill@sask.usask.ca
Asselin
J
J
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D015237:Dinoprost / Q000008:administration & dosage* / Q000502:physiology; D015232:Dinoprostone / Q000008:administration & dosage* / Q000502:physiology; D005334:Fever / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D006439:Hemodynamics / Q000187:drug effects; D002534:Hypoxia, Brain / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D015230:Prostaglandin D2 / Q000008:administration & dosage* / Q000502:physiology; D051381:Rats
D015237:Dinoprost; D015232:Dinoprostone; D015230:Prostaglandin D2