The hydA gene encoding the H(2)-evolving hydrogenase of Clostridium perfringens: molecular characterization and expression of the gene.
A putative hydrogenase (hydA) gene of Clostridium perfringens encodes a protein with strong identity to Clostridium pasteurianum hydrogenase I. Disruption of the hydA gene abolished H(2) productivity, confirming its function. A putative butyrate kinase gene (buk) is adjacent to the hydA gene. When cultures were grown in medium with glucose, 1.8-kb hydA and 2.1-kb buk transcripts and a 3. 9-kb transcript hybridized with both hydA and buk-probe were detectable in all the exponential growth phases. In medium without glucose, these transcripts were decreased rapidly after the mid-exponential phase. These results suggest that the transcription of these two genes is probably regulated by a similar mechanism in response to glucose availability.
Kaji
M
M
Department of Hospital Pharmacy, Faculty of Medicine, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa, Japan.
Taniguchi
Y
Y
Matsushita
O
O
Katayama
S
S
Miyata
S
S
Morita
S
S
Okabe
A
A
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D003016:Clostridium perfringens / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D017353:Gene Deletion; D005798:Genes, Bacterial; D005947:Glucose; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000096:biosynthesis / Q000235:genetics*; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000096:biosynthesis / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D007506:Iron-Sulfur Proteins; D012329:RNA, Bacterial; D006859:Hydrogen; C118830:iron hydrogenase; D006864:Hydrogenase; D017851:Phosphotransferases (Carboxyl Group Acceptor); C019285:butyrate kinase; D005947:Glucose