title: Geomicrobiology of subglacial ice above Lake Vostok, Antarctica.
abstract: Data from ice 3590 meters below Vostok Station indicate that the ice was accreted from liquid water associated with Lake Vostok. Microbes were observed at concentrations ranging from 2.8 x 10(3) to 3.6 x 10(4) cells per milliliter; no biological incorporation of selected organic substrates or bicarbonate was detected. Bacterial 16S ribosomal DNA genes revealed low diversity in the gene population. The phylotypes were closely related to extant members of the alpha- and beta-Proteobacteria and the Actinomycetes. Extrapolation of the data from accretion ice to Lake Vostok implies that Lake Vostok may support a microbial population, despite more than 10(6) years of isolation from the atmosphere.
authors.affiliation: Department of Biological Sciences, Montana State University, Bozeman, MT 59717, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Priscu
authors.forename: E E
authors.initials: EE
authors.lastname: Adams
authors.forename: W B
authors.initials: WB
authors.lastname: Lyons
authors.forename: M A
authors.initials: MA
authors.lastname: Voytek
authors.forename: D W
authors.initials: DW
authors.lastname: Mogk
authors.forename: R L
authors.initials: RL
authors.lastname: Brown
authors.forename: C P
authors.initials: CP
authors.lastname: McKay
authors.forename: C D
authors.initials: CD
authors.lastname: Takacs
authors.forename: K A
authors.initials: KA
authors.lastname: Welch
authors.forename: C F
authors.initials: CF
authors.lastname: Wolf
authors.forename: J D
authors.initials: JD
authors.lastname: Kirshtein
authors.forename: R
authors.initials: R
authors.lastname: Avci
mesh_terms: D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D020459:Genes, rRNA; D007053:Ice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008903:Minerals / Q000032:analysis; D011312:Pressure; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007053:Ice; D008903:Minerals; D012336:RNA, Ribosomal, 16S