title: Animation of the dynamical events of the elongation cycle based on cryoelectron microscopy of functional complexes of the ribosome.
abstract: Using three-dimensional cryoelectron microscopy, the binding positions of tRNA and elongation factors EF-G and EF-Tu (the latter complexed with aminoacyl tRNA and GTP) on the ribosome were determined in previous studies. On the basis of these studies, the dynamical events that take place in the course of the elongation cycle of protein synthesis have been animated. The resulting 3-min movie is accessible on the website of this journal (http://www. idealibrary.com). The following article provides a brief annotation of those frames of the movie for which experimental support is available.
authors.affiliation: Wadsworth Center, Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, New York 12201-0509, USA.
authors.forename: J
authors.initials: J
authors.lastname: Frank
authors.forename: A B
authors.initials: AB
authors.lastname: Heagle
authors.forename: R K
authors.initials: RK
authors.lastname: Agrawal
mesh_terms: D003198:Computer Simulation; D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000737:chemistry; D020407:Internet; D008958:Models, Molecular; D020653:Peptide Elongation Factor G / Q000737:chemistry; D010444:Peptide Elongation Factor Tu / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry; D012358:RNA, Transfer, Met / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry*; D012984:Software
chemical_list: D020653:Peptide Elongation Factor G; D012333:RNA, Messenger; D012358:RNA, Transfer, Met; C089238:fMet-tRNA(fMet); D010444:Peptide Elongation Factor Tu