Resolution of high-molecular-weight components in lipopolysaccharides of Escherichia coli, Morganella morganii, Citrobacter freundii and Citrobacter diversus strains with sodium dodecyl sulfate polyacrylamide gels.
The use of 0.5% sodium dodecyl sulfate in polyacrylamide separation gels allowed the resolution in several bands of high-molecular-mass components in smooth lipopolysaccharide of bacterial outer membrane from Escherichia coli, Morganella morganii, Citrobacter freundii and Citrobacter diversus. With or without 0.1% SDS, however, such a result was not possible.
Tavío
M M
MM
Microbiology, Department of Clinical Sciences, School of Medicine, University of Las Palmas de Gran Canaria, Spain. mtavio@infovia.ulpgc.es
Vila
J
J
Ruiz
J
J
Ruiz
J
J
Martín-Sánchez
A M
AM
Jiménez de Anta
M T
MT
D002954:Citrobacter / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004755:Enterobacteriaceae / Q000737:chemistry*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis*; D020613:Morganella morganii / Q000737:chemistry
D008070:Lipopolysaccharides