Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor.
Sacculi of Bacillus sphaericus CCM 2177 contain a secondary cell wall polymer which was completely extracted with 48% hydrofluoric acid. Nuclear magnetic resonance analysis showed that the polymer is composed of repeating units, as follows: -->3)-[4, 6-O-(1-carboxyethylidene)]( approximately 0. 5)-beta-D-ManpNAc-(1-->4)-beta-D-GlcpNAc-(1-->. The N-terminal part of the S-layer protein carrying S-layer homologous motifs recognizes this polymer as a binding site.
Ilk
N
N
Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, University of Agricultural Sciences, 1180 Vienna, Austria.
Kosma
P
P
Puchberger
M
M
Egelseer
E M
EM
Mayer
H F
HF
Sleytr
U B
UB
Sára
M
M
D001407:Bacillus / Q000737:chemistry* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008854:Microscopy, Electron; D010457:Peptidoglycan / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D010457:Peptidoglycan; D011108:Polymers; D011135:Polysaccharides, Bacterial; C055764:S-layer proteins; C402703:poly((4,6-O-(1-carboxyethylidene))-N-acetylmannosaminyl-1-4-N-acetylglucosaminyl-1-3-)