title: Two types of arrestins expressed in medaka rod photoreceptors.
abstract: Similar to visual arrestins of other vertebrates, two subtypes of medaka visual arrestins, KfhArr-R1 and KfhArr-C, are selectively expressed in rods and cones, respectively [Hisatomi et al. (1997) FEBS Lett. 411, 12-18]. We isolated a cDNA encoding the third arrestin, KfhArr-R2, from a medaka retinal cDNA library. Phylogenetic analysis of arrestin sequences suggests that KfhArr-R2 is classified into the rod arrestin subtype. In situ hybridization indicated that KfhArr-R2 mRNA is localized in most of the rod photoreceptors, suggesting that both KfhArr-R1 and -R2 are co-expressed in rods. Antisera against KfhArr-R2 recognized outer segments, but anti-KfhArr-R1 antisera reacted with cell bodies and synaptic termini in light-adapted rods. It seems likely that KfhArr-R1 and -R2 play different roles in rod photoreceptors.
authors.affiliation: Department of Earth and Space Science, Graduate School of Science, Osaka University, Machikaneyama-cyo 1-1, Toyonaka, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Imanishi
authors.forename: O
authors.initials: O
authors.lastname: Hisatomi
authors.forename: F
authors.initials: F
authors.lastname: Tokunaga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019393:Arrestin / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D029941:Fish Proteins; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017948:Retinal Rod Photoreceptor Cells / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014785:Vision, Ocular
chemical_list: D019393:Arrestin; D029941:Fish Proteins; C107704:KfhArr-R1 protein, Oryzias latipes; C491533:KfhArr-R2 protein, Oryzias latipes; D012333:RNA, Messenger