title: Characterization of echovirus 25 (ECV 25) in the VP1/2A gene junction region. Brief report.
abstract: Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus representatives (phylogenetic analysis). The analysis indicated that the sequences of recent isolates have drifted over time distinctly away from the prototype strain sequence. Genetic drift may change biological features of isolates possibly leading to new antigenic variants.
authors.affiliation: Robert Koch-Institut, Berlin, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Künkel
authors.forename: E
authors.initials: E
authors.lastname: Schreier
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004279:DNA, Viral; D004457:Echovirus Infections / Q000821:virology; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus