title: Ovine pestiviruses: their taxonomic status revealed by palindromic nucleotide substitutions.
abstract: We examined previously identified border disease virus (BDV) strains by using a newly proposed genotyping procedure based on palindromic nucleotide substitutions (PNS) in the 5'-untranslated region (UTR), and found 22 (41.5%) out of 53 strains of BDV in the nucleotide sequence databases are not of BDV. All the 22 ovine pestivirus strains were allocated to the BVDV species according to the PNS, and were compared with reference strains of pestivirus 1 (BVDV-Ia,-Ib, and-Ic genovars), pestivirus 2 (BVDV-II genovar), pestivirus 3 (BDV) and pestivirus 4 (CSFV), respectively. Ten strains (Weybridge, A553, B1056, D771/1, D861, D1120/1, D1432/P, Q1161/1, Q1161/2, 114817) showed a palindromic structure in the 5'-UTR characteristic to the BVDV-Ia genovar, three strains (7535, 7546, 7548) were characteristic to the BVDV-Ib genovar, and nine strains (BD-78, 59386, SCP, Lees, C413, 167237, 168149, 173157, 175375) belonged to the BVDV-II genovar.
authors.affiliation: lnstitute of Special Pathology and Veterinary Medical Clinic, Faculty of Veterinary Medicine, The University of Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Giangaspero
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry*; D005838:Genotype; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010576:Pestivirus / Q000145:classification* / Q000235:genetics; D018182:Pestivirus Infections / Q000662:veterinary / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*
chemical_list: D004279:DNA, Viral