Synthetic phytanyl-chained glycolipid vesicle membrane as a novel matrix for functional reconstitution of cyanobacterial photosystem II complex.
The vesicles composed of synthetic phytanyl-chained glycolipid and natural sulfoquinovosyldiacylglycerol at 9:1 molar ratio were successfully applied to functional reconstitution of photosystem II complex (PS II) from a thermophilic cyanobacterium. The synthetic glycolipid employed was one of our model archaeal diether lipids, 1, 3-di-O-phytanyl-2-O-(beta-D-maltotriosyl)glycerol. The light-induced oxygen-evolving activity of PS II reconstituted in the glycolipid vesicles was approximately 6-fold higher than that reconstituted in several phosphatidylcholine vesicles. The present results reveal the first evidence that a well-designed synthetic glycolipid is effective for the functional reconstitution of complicated and labile membrane protein complexes, such as PS II.
Baba
T
T
Department of Polymer Physics, National Institute of Materials and Chemical Research (NIMC), 1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
Minamikawa
H
H
Hato
M
M
Motoki
A
A
Hirano
M
M
Zhou
D
D
Kawasaki
K
K
D000458:Cyanobacteria / Q000378:metabolism*; D005614:Freeze Fracturing; D006017:Glycolipids / Q000138:chemical synthesis* / Q000737:chemistry*; D008027:Light; D008563:Membrane Lipids / Q000138:chemical synthesis / Q000737:chemistry; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D010100:Oxygen / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure
D006017:Glycolipids; D008563:Membrane Lipids; D008567:Membranes, Artificial; D045322:Photosynthetic Reaction Center Complex Proteins; D010100:Oxygen