Increased expression of mitochondrial respiratory enzymes in the brain of activated epilepsy-prone El mice.
The El mouse is an internationally registered animal model for hereditary temporal lobe epilepsy. When the mice receive weekly vestibular stimulation, e.g., 30 "tosses" 10-15 cm vertically, they start to convulse after 6-7 weeks on application of appropriate vestibular stimulation. The aim of this study was to explore the pathogenesis of the disease. By means of differential mRNA display we have screened five cDNAs which were upregulated in the brain of activated El mice given repeated vestibular stimulation. The differential expression of two (DD7/8 and DD8/24) cDNAs could be confirmed by Northern analyses. Sequence of the clones revealed that they were associated with mitochondrial respiratory enzymes, i.e., type 1 subunit of cytochrome c oxidase and a precursor of type 1 subunit of NADH dehydrogenase. The expression of these two genes was significantly increased in the El mice, which were activated by periodically repeated vestibular stimulation. The increased expression of these two genes may reflect increased demand for energy due to neuronal activation caused by repeated vestibular stimulation.
Yamada
Y
Y
Nagoya University Bioscience Center, Chikusa, Nagoya, Japan.
Nakano
K
K
D000818:Animals; D001921:Brain / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000235:genetics; D004833:Epilepsy, Temporal Lobe / Q000201:enzymology* / Q000209:etiology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D009245:NADH Dehydrogenase / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV