Development and evaluation of a new canine myocardial infarction model using a closed-chest injection of thrombogenic material.
A new canine myocardial infarction model using thrombi induced by closed-chest injection of thrombin and autogenous blood with fibrinogen into coronary arteries was developed. Occlusive thrombi were formed in all treated animals. Occluded vessels did not spontaneously reperfuse 1 day after occlusion, but did so within 3 days. Infarction was confirmed by increased levels of creatine kinase-MB, glutamate-oxaloacetate transaminase and a-hydroxybutyrate dehydrogenase. Additionally, the left ventricular ejection fraction (LVEF) decreased within 0.5 h after occlusion and had not improved 4 weeks later. After 1 week, extensive transmural anteroinferior myocardial infarction was observed and heart mass had increased. By 4 weeks after occlusion, pulmonary capillary wedge pressure and central venous pressure were increased, and oxygen pressure was decreased. Dropout of nuclei in cardiomyocytes and increased amount of collagen fiber were observed in myocardial infarct regions of hearts excised 4 weeks after occlusion. This canine model may be useful and convenient in evaluating treatment efficacy and the long-term outcome of acute myocardial infarction.
Suzuki
M
M
Applied Pharmacology Research, Pharmacology Laboratories, Yamanouchi Pharmaceutical Co. Ltd, Tsukuba, Ibaraki, Japan.
Asano
H
H
Tanaka
H
H
Usuda
S
S
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure; D001804:Blood Transfusion, Autologous; D003327:Coronary Disease / Q000097:blood / Q000201:enzymology / Q000473:pathology; D003328:Coronary Thrombosis / Q000097:blood / Q000201:enzymology / Q000473:pathology; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D004285:Dogs; D004562:Electrocardiography; D005340:Fibrinogen; D006339:Heart Rate; D006884:Hydroxybutyrate Dehydrogenase / Q000097:blood; D007269:Injections, Intra-Arterial; D007527:Isoenzymes; D009203:Myocardial Infarction / Q000097:blood* / Q000139:chemically induced* / Q000201:enzymology; D009929:Organ Size; D011669:Pulmonary Wedge Pressure; D013318:Stroke Volume; D013917:Thrombin; D014654:Vascular Patency
D007527:Isoenzymes; D005340:Fibrinogen; C035749:2-hydroxybutyrate dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D013917:Thrombin