title: Epipodite and fat cells as sites of hemoglobin synthesis in the branchiopod crustacean Daphnia magna.
abstract: In contrast to the malacostracan crustaceans that use hemocyanin as the oxygen carrier, a number of branchiopod crustaceans, such as the water flea Daphnia magna, utilize hemoglobin (Hb) as the respiratory protein. By means of in situ hybridization (ISH) techniques with subsequent signal amplification using catalyzed reporter deposition, sites of Hb synthesis were localized in Daphnia magna. Based on a previously reported Hb-cDNA sequence, a specific ISH probe was designed and hybridized with the Hb-mRNA in histological sections of adult D. magna. The detection of Hb-mRNA was tissue specific and revealed that Hb is synthesized in fat cells, which play a role in fat and glycogen metabolism, and in epithelial cells of the epipodites, which are involved in osmoregulation. Sites of Hb synthesis have been identified in several invertebrate phyla, including Annelida and Nematoda. However, this is the first example in the class Crustacea, and only the second in the phylum Arthropoda.
authors.affiliation: Klinische und Experimentelle Pathologie, Forschungszentrum Borstel, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Goldmann
authors.forename: B
authors.initials: B
authors.lastname: Becher
authors.forename: K H
authors.initials: KH
authors.lastname: Wiedorn
authors.forename: R
authors.initials: R
authors.lastname: Pirow
authors.forename: M E
authors.initials: ME
authors.lastname: Deutschbein
authors.forename: E
authors.initials: E
authors.lastname: Vollmer
authors.forename: R J
authors.initials: RJ
authors.lastname: Paul
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D003621:Daphnia / Q000378:metabolism*; D006454:Hemoglobins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic; D014882:Water-Electrolyte Balance
chemical_list: D015342:DNA Probes; D006454:Hemoglobins; D012333:RNA, Messenger