[Effect of carbon, nitrogen and phosphorus nutrition on the R, S, and M dissociants of Pseudomonas aeruginosa in mixed cultures].
The effect of glucose, nitrate, and phosphate on the stationary-phase growth characteristics of R, S, and M dissociants of the hydrocarbon-oxidizing strain P. aeruginosa K-2 was studied. The optimal concentrations of glucose and phosphate providing for at least 90% of the maximal culture density were found to be 2-7% glucose and 0.02-0.12% phosphate. The main factor that determined the proportion of dissociants in bacterial populations was the initial concentration of phosphate. The fraction of R dissociant in populations increased linearly with the concentration of glucose and varied nonlinearly with the concentration of phosphate in the growth medium. The fraction of M dissociant depended solely on the concentration of phosphate in a manner inverse to that typical of R dissociant. In glucose-deficient media containing sufficient amounts of phosphorus, S dissociant prevailed over R dissociant.
Maksimov
V N
VN
Moscow State University, Vorob'evygory, Russia.
Mil'ko
E S
ES
Il'inykh
I A
IA
D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D009584:Nitrogen / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen
Vliianie uglerodnogo, azotnogo i fosfornogo pitaniia na rost R-, S- i M-dissotsiantov Pseudomonas Aeruginasa v smeshannykh kul'turakh.