A mixture of alpha-helical and 3(10)-helical conformations for involucrin in the human epidermal corneocyte envelope provides a scaffold for the attachment of both lipids and proteins.
Involucrin plays an important role in the lipid and protein compound envelopes of mammalian epidermal corneocytes. In the present study, model peptides containing the consensus repeating units PEQQEGQLEL and LEQQEGQLEH, found in the central region of human involucrin, were studied by circular dichroism spectroscopy, molecular modeling, and energy minimization. These peptides have intrinsic alpha-helix-forming properties as indicated by their circular dichroic spectra obtained in the presence of 2,2,2-trifluoroethanol. Peptide (LEQQEGQLEH)(3) had an alpha-helix content of 100% in 100% 2, 2,2-trifluoroethanol at 0 degrees C. The energy-minimized alpha-helix showed that only 50% of the glutamate side chains may be available for the attachment of lipids. However, when a 3(10)-helix was assumed for the GQL or GQLE residues in LEQQEGQLEH, all of the glutamate side chains were arrayed on one face of the helix, and all of the glutamine side chains were arrayed on the opposite face. A similar result was obtained when the nonhelical part of PEQQEGQLEL was assumed to contain a beta-turn III, which is equivalent to a short portion of 3(10)-helix. The results of this study suggest that when the central segment of human involucrin is predominantly alpha-helical, accompanied by short 3(10)-helical segments, the protein can function as a scaffold for the attachment of both lipids and proteins.
Lazo
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ND
Department of Dermatology, University of Iowa, Iowa City, Iowa 52242, USA. noellazo@uiowa.edu
Downing
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DT
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D050356:Lipid Metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry* / Q000502:physiology; D017433:Protein Structure, Secondary; D011506:Proteins / Q000378:metabolism*; D013329:Structure-Activity Relationship
D011498:Protein Precursors; D011506:Proteins; C032253:involucrin