Analysis of anatoxin-a in freshwaters by automated on-line derivatization-liquid chromatography-electrospray mass spectrometry.
Anatoxin-a is a toxin produced from cyanobacterial blooms in freshwaters. In order to determine trace anatoxin-a in freshwaters, an automated on-line derivatization procedure with fluorenyl methylchloroformate using liquid chromatography-electrospray ionization mass spectrometry was developed. Anatoxin-a was extracted using solid-phase extraction with adequate recovery (75.7+/-7.2%, n=6) at 20 ng/l. The limits of quantification and detection were calculated to be 15.2 ng/l and 2.1 ng/l, respectively, using selected ion monitoring.
Takino
M
M
Yokogawa Analytical Systems Inc., Osaka, Japan.
Daishima
S
S
Yamaguchi
K
K
D001427:Bacterial Toxins / Q000032:analysis*; D002853:Chromatography, Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D000087522:Cyanobacteria Toxins; D005466:Fluorocarbons / Q000032:analysis; D005618:Fresh Water / Q000032:analysis*; D007202:Indicators and Reagents; D008387:Marine Toxins / Q000032:analysis*; D013058:Mass Spectrometry; D052998:Microcystins; D009862:Online Systems; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014326:Tropanes
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D005466:Fluorocarbons; D007202:Indicators and Reagents; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; C033093:perfluoropropionic acid; C509783:anatoxin a