ExoT of cytotoxic Pseudomonas aeruginosa prevents uptake by corneal epithelial cells.
The presence of invasion-inhibitory activity that is regulated by the transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been proposed. The results of this study show that both ExoT and ExoS, known type III secreted effector proteins of P. aeruginosa that are regulated by ExsA, possess this activity. Invasion was reduced 94.4% by ExoT and 96.0% by ExoS. Invasion-inhibitory activity is not linked to ADP-ribosylation activity, at least for ExoS, since a noncatalytic mutant also inhibits uptake by an epithelial cell line (invasion was reduced 96. 0% by ExoSE381A).
Cowell
B A
BA
School of Optometry, University of California, Berkeley, California 94720, USA.
Chen
D Y
DY
Frank
D W
DW
Vallis
A J
AJ
Fleiszig
S M
SM
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D019573:Epithelium, Corneal / Q000382:microbiology*; D005798:Genes, Bacterial; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity*; D011817:Rabbits; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D014774:Virulence / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C095092:ExsA protein, bacteria; D015534:Trans-Activators