Chronic ethanol enhances muscarinic receptor-mediated activator protein-1 (AP-1) DNA binding in cerebellar granule cells.
In this study we investigated effects of acute and chronic ethanol exposure on carbachol-induced activator protein-1 (AP-1) DNA binding in rat cerebellar granule cells. Acute ethanol application did not alter, whereas chronic ethanol exposure potentiated the carbachol-induced AP-1 DNA binding. The protein composition of the AP-1 transcription factor complex activated by carbachol stimulation of muscarinic receptors was analysed in control and chronic ethanol-exposed cells using a supershift assay with specific antibodies against c-Fos, Fos B, c-Jun, Jun B and Jun D proteins. Supershift analysis revealed that the carbachol-induced AP-1 complex was composed predominantly of Jun D and c-Fos. The composition of the AP-1 complex activated by carbachol in chronic ethanol-exposed cells did not differ from control. These findings indicate that chronic ethanol treatment can modulate carbachol-induced AP-1 DNA binding activity in cerebellar granule cells.
Cebers
G
G
Department of Clinical Neuroscience, Division of Drug Dependence Research, Karolinska Institutet, Building L1:01, SE-17176, Stockholm, Sweden.
Hou
Y
Y
Cebere
A
A
Terenius
L
L
Liljequist
S
S
D000818:Animals; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D017874:Immediate-Early Proteins / Q000037:antagonists & inhibitors / Q000276:immunology; D009841:Oligonucleotides / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects*; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D002492:Central Nervous System Depressants; D017874:Immediate-Early Proteins; D009841:Oligonucleotides; D011976:Receptors, Muscarinic; D018808:Transcription Factor AP-1; D000431:Ethanol; D004247:DNA