Discordance between fetal RhD typing using molecular methods and neonatal typing with serology.
Polymerase chain reaction (PCR)-based genotyping on amniotic fluid in an RhD-negative alloimmunized woman predicted an RhD-negative fetal blood type. The neonate was RhD-positive and developed hemolytic disease. Discrepant results were also observed on paternal testing. PCR analysis with a different set of primers correctly predicted the RhD-positive fetal and paternal blood type. Use of more than one set of primers and parental testing can avoid some of the problems associated with use of PCR genotyping.
Van Den Veyver
I B
IB
Department of Obstetrics and Gynecology, Divisions of Maternal Fetal Medicine, Baylor College of Medicine, Houston, TX, USA.
Yankowitz
J
J
Subramanian
S B
SB
Dorman
K F
KF
Moise
K J
KJ
D000328:Adult; D000653:Amniotic Fluid; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004899:Erythroblastosis, Fetal / Q000628:therapy; D005188:False Negative Reactions; D005260:Female; D005838:Genotype; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012203:Rh Isoimmunization; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
D012204:Rh-Hr Blood-Group System