Intragraft CD45 RO gene expression is an early marker to detect small bowel allograft rejection in rats.
Wistar Furth (WF) intestinal allografts were transplanted into Sprague-Dawley (SD) rats. Recipients were randomly allocated into the following groups: (1) no treatment; (2) cyclosporine (CsA) 6 mg/kg/day, daily, subcutaneously (s.c.; full-dose therapy); (3) CsA 3 mg/kg/day, daily, s.c. (half-dose therapy); and (4) CsA 3 mg/kg/day, daily, s.c. + Tripterygium Wilfordii Hook. WF (TW) 3 mg/kg/day, daily, s.c. WF rats with intestinal autografts were used as controls. CD45RO intragraft expression and its index (CD45RO/CD45), measured by reverse transcription polymerase chain reaction (RT-PCR), were significantly elevated in untreated and half-dose CsA-treated allografts as early as postoperative day (POD) 4, when rejection of intestinal allografts was not detected by routine pathology. Intestinal permeability measured by Tc-DTPA radioassay was significantly elevated in untreated allografts on POD 6. Histology showed that there was severe rejection in untreated intestinal allografts and mild rejection in allografts treated with a half dose of CsA on POD 6. There was a normal CD45RO expression, permeability, and histology in intestinal allografts treated with either a full dose of CsA or a half dose of CsA + TW. These data indicate that CD45RO intragraft gene expression is an early marker to detect intestinal allograft rejection in rats.
Wang
P
P
Department of Surgery, Tianjin Medical University, Tianjin Medical University Hospital and Tianjin Research Institute of Experimental Surgery, Tianjin, P. R. China.
Zhu
L
L
Liu
T
T
Zhang
X
X
Qiu
Y
Y
D000818:Animals; D015415:Biomarkers; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D006084:Graft Rejection / Q000175:diagnosis*; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D011920:Rats, Inbred WF; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D017493:Leukocyte Common Antigens