title: Effect of fusidic acid on the hepatic cytochrome P450 enzyme system.
abstract: OBJECTIVE
  To investigate the effects of fusidic acid therapy on the hepatic cytochrome P450 (CYP450) enzyme system.
  
  
  METHODS
  Thirty HIV-seropositive L-methadone-substituted i.v. drug abusers (stage CDC/WHO B2 - 3 with CD4+-counts ranging from 65 to 293/microl) were randomized into 3 groups (A - C). Ten patients were treated with fusidic acid 500 mg/day over a period of 14 (group A) or 28 days (group B), respectively. Patients in group C served as a control group and did not receive any medication apart from L-methadone. In order to investigate the hepatic monooxygenase system, pharmacokinetics were determined in all patients before initiation and 14 and 28 days after starting therapy with fusidic acid. The concentration of antipyrine and its 3 main metabolites (norantipyrine (NORA), 4-hydroxyantipyrine (OHA), 3-hydroxymethylantipyrine (HMA)) in plasma and urine were measured by high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  No effects on antipyrine pharmacokinetics and pharmacokinetics of antipyrine metabolites were found in group A after 14 days of fusidic acid intake and in the control group without therapy. However, in contrast an activation of the CYP450 enzyme system was observed in group B after 28 days of fusidic acid therapy with an increase of total antipyrine clearance (43.0 +/- 7.62 ml/min to 51.0 +/- 9.03 ml/min) as well as clearances to all metabolites (NORA 7.11 +/- 1.75 to 8.60 +/-2.10 ml/min, OHA 11.5 +/- 2.89 to 14.0 +/- 3.97 ml/min, HMA 4.05 +/- 0.99 to 4.94 +/- 1.27 ml/min). Antipyrine half-life was significantly reduced (12.3 +/- 2.8 h to 9.4 +/- 2.2 h) and some patients developed clinical signs of L-methadone underdosage.
  
  
  CONCLUSIONS
  Our results suggest that fusidic acid has a time-dependent activating effect on the CYP450 enzyme system. Especially in treatment of patients who are frequently under multidrug regimens such as HIV-positive patients drug interactions should be taken into consideration.
authors.affiliation: Department of Dermatology, Venerology, and Allergology, University of Essen, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Reimann
authors.forename: B
authors.initials: B
authors.lastname: Barthel
authors.forename: J K
authors.initials: JK
authors.lastname: Rockstroh
authors.forename: D
authors.initials: D
authors.lastname: Spatz
authors.forename: N H
authors.initials: NH
authors.lastname: Brockmeyer
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D000983:Antipyrine / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects; D005672:Fusidic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006679:HIV Seropositivity / Q000201:enzymology*; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008691:Methadone / Q000627:therapeutic use; D009021:Morphine Dependence / Q000201:enzymology* / Q000534:rehabilitation; D009294:Narcotics / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D009294:Narcotics; D005672:Fusidic Acid; D003577:Cytochrome P-450 Enzyme System; D000983:Antipyrine; D008691:Methadone