A receptor-based bioassay for quantitative detection of gallium.
The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrixes. We have synthesized new protein conjugates based on 4-(2-pyridylazo) ligands. The conjugates were successfully employed for the detection of gallium in biological matrixes using a nonantibody-based sandwich assay format. The recovery level obtained was between 97 and 101.3 with a relative standard deviation of less than 5%. The assay resulted in a detection limit of 5 x 10(-8) M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provided adequate accuracy for gallium applicable for animal physiology and clinical toxicology.
Xu
H
H
Department of Chemistry, State University of New York at Binghamton 13902, USA.
Lee
E
E
Sadik
O A
OA
Bakhtiar
R
R
Drader
J
J
Hendrikson
C
C
D001681:Biological Assay / Q000379:methods*; D005708:Gallium / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism*; D015203:Reproducibility of Results; D012118:Resorcinols / Q000378:metabolism*; D012680:Sensitivity and Specificity
D008565:Membrane Proteins; D012118:Resorcinols; D005708:Gallium; C028398:4-(2-pyridylazo)resorcinol