Deficiency of intercellular adhesion molecule 1 fails to mitigate selective neuronal death after transient global ischemia.
Recent studies have shown a crucial role of intercellular adhesion molecule 1 (ICAM-1) in expansion of infarction after focal cerebral ischemia. The purpose of the present study was to assess whether ICAM-1 is involved in selective neuronal vulnerability and reactive gliosis after transient forebrain ischemia. ICAM-1 knockout mice and wild-type mice were subjected to transient forebrain ischemia for 5, 10 or 15 min, and the hippocampus and caudoputamen were examined 7 days later with conventional histological and immunohistochemical methods. Bilateral common carotid artery occlusion with less than 10% of baseline cortical microperfusion for 10 or 15 min resulted in ischemic neuronal damage in the hippocampus and caudoputamen. The frequency and the severity of neuronal damage were similar in wild-type and knockout mice. Proliferation of reactive astrocytes in the hippocampus was also similar in both types of mice. Therefore, it is highly unlikely that ICAM-1 plays a key role in delayed neuronal death after transient global ischemia or in astroglial responses after ischemic neuronal injury.
Kitagawa
K
K
Division of Strokology, Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine (A-8), 2-2 Yamadaoka, Suita, Japan. kitagawa@medone.med.osaka-u.ac.jp
Matsumoto
M
M
Ohtsuki
T
T
Kuwabara
K
K
Mabuchi
T
T
Yagita
Y
Y
Hori
M
M
Yanagihara
T
T
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000503:physiopathology*; D002341:Carotid Artery Thrombosis / Q000378:metabolism / Q000503:physiopathology; D002421:Caudate Nucleus / Q000378:metabolism / Q000503:physiopathology*; D016923:Cell Death; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011699:Putamen / Q000378:metabolism / Q000503:physiopathology*; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology
D005904:Glial Fibrillary Acidic Protein; D018799:Intercellular Adhesion Molecule-1