Inhibition of transcription of class II, but not class III and I, genes in Xenopus postblastular embryos overexpressed with the TBP-binding protein, Dr1 (NC2beta).
Dr1 (NC2beta) is known to effectively repress transcription of class II genes, and much less effectively class III genes, but not class I genes through its binding to the TATA-binding protein (TBP), which is the major component of the basal transcription factor for polymerases II, III, and I. Previously, we isolated Xenopus Dr1 cDNA, and demonstrated that its mRNA is transcribed in oocytes and is inherited into early embryos, but its level decreases in later stages. Here, we overexpressed Xenopus Dr1 in Xenopus embryos and, found that the overexpression significantly reduces the levels of poly(A), cytoskeletal actin and histone H4 mRNAs, and the labeling of heterogeneous mRNA-like RNA in postblastular embryos, without affecting tRNA and rRNA syntheses. These results indicate that the overexpressed Dr1 specifically down-regulates the transcription of class II, but not class III and I, genes, and suggest that Dr1 plays an important role in the control of transcription in Xenopus embryogenesis.
Nagano
M
M
Laboratory of Molecular Embryology, Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Shiokawa
K
K
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000145:classification / Q000235:genetics*; D005260:Female; D005775:Gastrula / Q000378:metabolism; D015870:Gene Expression; D006657:Histones / Q000235:genetics; D066298:In Vitro Techniques; D008845:Microinjections; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics / Q000378:metabolism
D000199:Actins; D017931:DNA Primers; D006657:Histones; D010750:Phosphoproteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014157:Transcription Factors; C075995:down-regulator of transcription 1; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases