A simple estimation method for the embryonic toxicity of plastic wrapping sheets using an egg shell-free culture system of chick embryos.
To estimate the embryonic toxicity of food wrapping films, the shell-free chick embryos were cultured using plastic wrapping sheets like a suspending hammock. Polyethylene (PE) wrapping sheets with no additives showed much higher survivability than polyvinylchloride (PVC) wrapping sheets including plasticizers. Furthermore when a PVC wrapping sheet was autoclaved at 110 degrees C for 30 min, the embryonic survivability significantly decreased compared with the untreated PVC wrapping sheet. These observations suggest that the egg shell-free culture method using a plastic wrapping sheet is a useful estimation method for the embryonic toxicity of food wrapping film.
Okai
Y
Y
Department of Human Life Science, Osaka Kun-Ei Women's College, Japan.
Higashiokai
K
K
D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D018857:Food Packaging; D010968:Plasticizers / Q000633:toxicity*; D020959:Polyethylene / Q000633:toxicity*; D011143:Polyvinyl Chloride / Q000633:toxicity*; D013696:Temperature; D013997:Time Factors; D014022:Tissue Survival / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods*
D010968:Plasticizers; D011143:Polyvinyl Chloride; D020959:Polyethylene