Selectin-carbohydrate interactions in shear flow.
Hydrodynamic shear creates mechanical stresses on selectin bonds, modulating affinity and kinetic parameters. Chemical modification of sialyl Lewis(x) increases the strength of L-selectin bonds without altering recognition, suggesting that mechanical and biorecognition characteristics are separable. L-selectin bond formation rates may be strongly influenced by sulfate orientation in sulfo sialyl Lewis(x).
Lawrence
M B
MB
Department of Biomedical Engineering, University of Virginia, Health Sciences Center, Box 377, Charlottesville, VA 22908, USA. mbl2a@virginia.edu.
D002241:Carbohydrates / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D012212:Rheology; D019087:Selectins / Q000737:chemistry*
D002241:Carbohydrates; D008024:Ligands; D019087:Selectins