The structure of the carbohydrate backbone of core-lipid A region ofthe lipopolysaccharides from Proteus mirabilis wild-type strain S1959 (serotype O3) and its Ra mutant R110/1959.
The following structure of core-lipid A region of the lipopolysaccharide (LPS) from Proteus mirabilis strain 1959 (serotype O3) and its rough mutant R110/1959 (Proteus type II core) was determined using NMR and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, and of the products of alkaline deacylation of the LPS: Incomplete substitutions are indicated by italics. All sugars are in pyranose form, alpha-Hep is the residue Lglycero-alpha-Dmanno-Hep, alpha-DD-Hep is the residue Dglycero-alpha-Dmanno-Hep. The differences with the previously reported structures are discussed.
Vinogradov
E
E
Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark. evv@crc.dk
Radziejewska-Lebrecht
J
J
Kaca
W
W
D000215:Acylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008050:Lipid A / Q000737:chemistry* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011513:Proteus mirabilis / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D011522:Protons; D011700:Putrescine / Q000032:analysis; D012703:Serotyping; D012972:Sodium Hydroxide / Q000378:metabolism; D013095:Spermidine / Q000032:analysis
D008050:Lipid A; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D011522:Protons; D012972:Sodium Hydroxide; D013095:Spermidine; D011700:Putrescine