Reevaluation of the promoter structure of the class 3 flagellar operons of Escherichia coli and Salmonella.
Flagellar class 3 operons of Escherichia coli and Salmonella are transcribed by RNA polymerase containing sigma 28. The consensus sequence of the sigma 28-dependent promoters was believed to be TAAA N15 GCCGATAA. In this study, we found that the E. coli genome contains a large number of sequences homologous to this consensus. However, we showed that they do not always exert a sigma 28-dependent promoter activity. We compare more carefully the sequences of the class 3 flagellar promoters and propose a revised structure of the sigma 28-dependent promoters as TAAAGTTT N11 GCCGATAA.
Ide
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Faculty of Applied Biological Science, Hiroshima University, Japan.
Ikebe
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Kutsukake
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D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012475:Salmonella / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
C403898:RNA polymerase sigma(28); D012321:DNA-Directed RNA Polymerases