title: Human platelet antigens: typing by PCR using sequence-specific primers and their distribution in blood donors resident in Wales.
abstract: We developed and validated HPA-1 to HPA-6 typing by PCR-SSP using a combination of established, modified and newly designed sequence-specific primers. We confirmed that the PCR primer mixtures functioned under the same PCR conditions as our standard HLA-A, -B, -C, -DR, -DQ PCR-SSP typing system. This allows concurrent testing for both HPA and HLA specificities and is therefore the system of choice for both clinical and large-scale blood donor panel HPA and HLA typing by PCR-SSP. Test validation included typing a population of blood donors living in Wales. These HPA frequencies were consistent with those of other European Caucasoid populations. HPA-4b and -6b were absent and HPA-5b, which shows some frequency variation, had a phenotype frequency of 18.9% (allele frequency 0. 0973), being close to that of the Dutch (19.7%) and Austrian (20.4%) populations and almost twice that found in Finns (10.0%). HPA genotype frequencies showed a good fit to Hardy-Weinberg equilibrium, further supporting the validity of our typing method.
authors.affiliation: Regional Tissue Typing Laboratory, Welsh Blood Sevice, Pontyclun, Wales, UK.
authors.forename: J
authors.initials: J
authors.lastname: Sellers
authors.forename: J
authors.initials: J
authors.lastname: Thompson
authors.forename: M G
authors.initials: MG
authors.lastname: Guttridge
authors.forename: C
authors.initials: C
authors.lastname: Darke
mesh_terms: D016824:Antigens, Human Platelet / Q000032:analysis* / Q000235:genetics; D001782:Blood Donors; D017931:DNA Primers; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D014852:Wales
chemical_list: D016824:Antigens, Human Platelet; D017931:DNA Primers