Rapid determination of citrinin in corn by fluorescence liquid chromatography and enzyme immunoassay.
A rapid assay procedure was developed for mycotoxin citrinin in corn using liquid-liquid extraction (LLE) cartridges. Ground corn was extracted with methylene chloride and 0.5 N phosphoric acid. The extract was added to an LLE cartridge containing a diatomaceous-earth adsorbant, previously impregnated with sodium bicarbonate solution. After aspiration to dryness, the cartridge was eluted with methanol-water (4 + 1), and aliquots were taken for quantitation by reversed-phase liquid chromatography with fluorescence detection. Recoveries of citrinin added to ground corn at 200-1600 ng/g ranged from 71.2 to 86.3%, with coefficients of variation between 4.1 and 10.6%. An indirect enzyme immunoassay was also evaluated, using sodium carbonate solution for extraction. Recoveries of citrinin added to ground corn at 200-2000 ng/g ranged from 53.2 to 67.2%, but the coefficients of variation varied between 18.4 and 51.5%. The LLE cartridge procedure offers the advantages of low solvent consumption and speed, and is amenable to automation.
Abramson
D
D
Agriculture and Agri-Food Canada, Cereal Research Center, Winnipeg, Canada.
Usleber
E
E
Märtlbauer
E
E
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002254:Carbonates; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D002953:Citrinin / Q000032:analysis*; D005453:Fluorescence; D007124:Immunoenzyme Techniques; D008752:Methylene Chloride; D010756:Phosphoric Acids; D011786:Quality Control; D012680:Sensitivity and Specificity; D003313:Zea mays / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D002254:Carbonates; D010756:Phosphoric Acids; D002953:Citrinin; C005686:sodium carbonate; D008752:Methylene Chloride; C030242:phosphoric acid