Reactive oxygen species production of neutrophils in patients with acute promyelocytic leukemia during treatment with all-trans retinoic acid.
We measured N-formyl-methionyl-leucyl-phenylalanine-induced reactive oxygen species production by neutrophils from three patients with acute promyelocytic leukemia during treatment with all-trans retinoic acid using a luminol-enhanced chemiluminescence assay. The maximum level of reactive oxygen species production during all-trans retinoic acid treatment was 58.8 +/- 2.3 x 10(4) (mean +/- SEM) counted photons per seconds (cps), which was significantly higher (p<0.0001) than that of neutrophils from health volunteers (13.3 +/- 2.3 x 10(4) cps).
Ohno
E
E
Second Department of Internal Medicine, Oita Medical University, Oita, Japan. eiji@oita-med.ac.jp
Ohtsuka
E
E
Saburi
Y
Y
Ono
K
K
Kikuchi
H
H
Nasu
M
M
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood* / Q000188:drug therapy*; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012016:Reference Values; D013997:Time Factors; D014212:Tretinoin / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D014212:Tretinoin; D008165:Luminol