title: Renal ischemia-induced increase in vascular permeability is limited by hypothermia.
abstract: The purpose of the present work was to evaluate the kallikrein-kinin system and effects of hypothermia during renal ischemia and reperfusion. Male C57BL/KSJmdb mice were subjected to 20 or 60 min ischemia for different periods of reperfusion. Our results demonstrate that short periods of ischemia followed by reperfusion did not cause significant alterations in kallikrein activity, Evans Blue (EB) extravasation, prokallikreins, myeloperoxidase activity or plasma creatinine concentration. Edema was evident at 1 h reperfusion in the treated mice, but returned to basal values after 24 h reperfusion. Kallikrein activities and EB extravasation showed a significant increase in 60 min ischemic mice. Myeloperoxidase activity in the kidney of the mice confirmed net infiltration in the group with 60 min ischemia and 24 h reperfusion. The generation of kinins and activation of matrix degrading enzymes by tissue kallikrein, liberated from both renal and infiltrated leukocytes, could be responsible at least in part for the damage observed in the kidney of mice subject to 60 min ischemia and reperfusion. The hypothermia significantly reduced the inflammatory process in the 60 min ischemic mice, and did prevent an increase in vascular permeability. Nevertheless, the tissue edema was not shown to change between normothermic and hypothermic ischemic mice.
authors.affiliation: PROSIVAD-CONICET, Cátedra de Fisiología, Facultad de Farmacia Bioquímica, Buenos Aires, Argentina.
authors.forename: M D
authors.initials: MD
authors.lastname: Carattino
authors.forename: F
authors.initials: F
authors.lastname: Cueva
authors.forename: A
authors.initials: A
authors.lastname: Zuccollo
authors.forename: J L
authors.initials: JL
authors.lastname: Monti
authors.forename: M
authors.initials: M
authors.lastname: Navarro
authors.forename: O L
authors.initials: OL
authors.lastname: Catanzaro
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D007035:Hypothermia / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D007610:Kallikreins / Q000378:metabolism; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury / Q000209:etiology
chemical_list: D007610:Kallikreins