title: Adhesion defects of antibody-mediated target cell binding of neonatal natural killer cells.
abstract: Neonates are unusually susceptible to herpes simplex virus infection, which may be explained in part by defects in killing of herpes simplex virus-infected cells by natural killer (NK) cell cytotoxicity and antibody-dependent cellular cytotoxicity. The mechanism for these defects remains poorly defined. We have for the first time used immunomagnetically enriched NK cells to explore neonatal NK cell phenotype and target cell adhesion. CD56-positive neonatal NK cells had markedly lower CD57 expression, but adult level expression of adhesive glycoproteins (CD18, CD44) and Fc receptor for IgG (CD16). Although the cells conjugated normally with target cells in the absence of antibody, antibody-mediated conjugation was significantly lower than that of NK cells from adults (p < 0.002). These results demonstrate intact adhesion in neonatal NK cell cytotoxicity. In contrast, defective neonatal antibody-dependent cellular cytotoxicity is caused, in part, by an adhesion defect in the presence of antibody.
authors.affiliation: Department of Pediatrics, University of California Medical School, San Francisco General Hospital, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kohl
authors.forename: M
authors.initials: M
authors.lastname: Sigouroudinia
authors.forename: E G
authors.initials: EG
authors.lastname: Engleman
mesh_terms: D000328:Adult; D000906:Antibodies / Q000276:immunology*; D019002:CD56 Antigen / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005312:Fetal Blood; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D011961:Receptors, Fc / Q000276:immunology; D018139:Simplexvirus
chemical_list: D000906:Antibodies; D019002:CD56 Antigen; D007074:Immunoglobulin G; D011961:Receptors, Fc