Intact and photomodified polycyclic aromatic hydrocarbons inhibit photosynthesis in natural assemblages of Lake Erie phytoplankton exposed to solar radiation.
Recently, there has been a trend toward less turbid water and greater light penetration in parts of western Lake Erie. This could lead to greater phototoxicity from sediment-bound polycyclic aromatic hydrocarbons. To test photosynthesis as a bioindicator of contaminant impacts on algae, water samples containing natural assemblages of phytoplankton were collected from the western and central basins of Lake Erie. These samples were incubated with 0.2 to 2 mg L(-1) anthracene or its photomodified product 1, 2-dihydroxyanthraquinone for 60 min in darkness or in 50% sunlight, to mimic exposure of phytoplankton in the photic zone of a mixed water column. Photosynthetic efficiency was determined from filtered phytoplankton immediately after exposure using a pulse-amplitude modulated chlorophyll fluorometer. Phytoplankton incubated with chemicals in the dark demonstrated chlorophyll fluorescence values similar to those of controls. However, exposure to anthracene or 1, 2-dihydroxyanthraquinone in sunlight diminished photosystem II photosynthetic efficiency and photosynthetic quantum yield in a concentration-dependent manner. Anthracene inhibited photosynthesis at lower concentrations than 1,2-dihydroxyanthraquinone, which is consistent with the different modes of action and toxic strengths of these two contaminants. These results demonstrate that phytoplankton in Lake Erie can be subject to phototoxicity from intact and photomodified polycyclic aromatic hydrocarbons after very short exposures. Further, chlorophyll fluorescence was found to be an effective bioindicator in the field for this form of chemical stress.
Marwood
C A
CA
Centre for Toxicology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
Smith
R E
RE
Solomon
K R
KR
Charlton
M N
MN
Greenberg
B M
BM
D015415:Biomarkers / Q000032:analysis; D002734:Chlorophyll / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods; D005453:Fluorescence; D010788:Photosynthesis / Q000187:drug effects*; D010839:Phytoplankton / Q000187:drug effects* / Q000502:physiology; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics / Q000633:toxicity*; D013472:Sunlight; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D002734:Chlorophyll