Modulation of lipid peroxidation and antioxidant enzymes in murine salivary gland by dietary fatty acid ethyl esters.
The present study was undertaken to investigate the effect of n-9, n-6, and n-3 dietary fatty acid ethyl esters on basal (uninduced) and Fe2+/ascorbate (induced) lipid peroxidation (LPO) in salivary gland (SG) of mice. Feeding n-3 ethyl ester polyunsaturated fatty acids (PUFA) increased the uninduced and induced LPO in SG homogenates. In contrast, feeding olive oil ethyl esters (n-9) significantly lowered the induced and uninduced LPO in SG tissue. Salivary gland susceptibility to LPO increased in the order of: olive oil < corn oil < safflower oil < n-3 ethyl esters. Olive oil esters in the diet increased primarily the 18:1 levels in SG tissue. Whereas feeding n-3 PUFA notably increased the superoxide dismutase (SOD) and catalase activities in SG homogenates, no significant changes were seen between n-9 and n-6 PUFA-fed mice. Lower levels of Vitamin E (Vit E) in the tissues of n-3 PUFA-fed mice indicate that the higher the dietary lipid unsaturation, the higher the requirement for Vit E in the diet. Our results indicate that, similar to other organs, salivary gland susceptibility to uninduced or induced oxidation depends on the source of dietary PUFA. In conclusion, feeding olive oil increases the resistance of SGs to induced and uninduced LPO.
Avula
C P
CP
Department of Medicine, The University of Texas Health Science Center, San Antonio 78284-7874, USA.
Fernandes
G
G
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D004952:Esters; D005296:Ferrous Compounds / Q000494:pharmacology; D005395:Fish Oils / Q000008:administration & dosage / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000097:blood / Q000378:metabolism; D008055:Lipids / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D000074242:Sunflower Oil; D013482:Superoxide Dismutase / Q000378:metabolism*; D014810:Vitamin E / Q000008:administration & dosage / Q000378:metabolism
D004042:Dietary Fats, Unsaturated; D004952:Esters; D005296:Ferrous Compounds; D005395:Fish Oils; D008054:Lipid Peroxides; D008055:Lipids; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; C051140:iron(II)-ascorbic acid complex; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D001205:Ascorbic Acid