Detection of measles specific IgG in oral fluid using an FITC/anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA).
An IgG antibody capture enzyme linked immunosorbent assay (GACELISA) for the detection of measles specific IgG in oral fluid was developed using an FITC/anti-FITC amplification system. The GACELISA was evaluated by testing paired oral fluid and serum samples from 787 subjects in an epidemiological study of measles in rural Ethiopia. Oral fluids were tested by GACELISA and corresponding serum samples by a sensitive indirect ELISA for measles IgG (Behring Enzygnost). By comparison with the serum measles IgG assay, the oral fluid GACELISA had a sensitivity of 97.4% (95% confidence intervals: 95.9, 98.2) and a specificity of 90.0% (81.9, 94.3), with no significant differences observed by age group. Total IgG concentrations were measured on a subset of 160 oral fluids by an in-house ELISA. This showed that false negative GACELISA results tended to occur in samples with low concentrations of total IgG, although the trend was not statistically significant. It is concluded that the overall performance of the GACELISA was satisfactory, showing close agreement to the serum ELISA, and has potential to serve as an easily transferable tool for large scale epidemiological studies as required for the World Health Organisation's programme for the global control of measles.
Nigatu
W
W
Ethiopian Health and Nutrition Research Institute, Addis Ababa.
Nokes
D J
DJ
Enquselassie
F
F
Brown
D W
DW
Cohen
B J
BJ
Vyse
A J
AJ
Cutts
F T
FT
D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D000918:Antibody Specificity; D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005002:Ethiopia / Q000453:epidemiology; D005069:Evaluation Studies as Topic; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D008457:Measles / Q000453:epidemiology / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D012424:Rural Population; D012463:Saliva / Q000276:immunology; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D016650:Fluorescein-5-isothiocyanate