Probing the microenvironment of intracellular bacterial pathogens.
The identification of bacterial genes regulated in response to the intracellular environment is crucial to the understanding of host-pathogen interactions. Several techniques have been developed to identify and characterize bacterial genes that are induced during the intracellular infection and, potentially, may play a role in pathogenesis. This review discusses the strategies that have been utilized to examine differential gene expression by bacterial pathogens during the intracellular infection. Furthermore, a number of the differentially expressed genes are described.
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Department of Microbiology and Immunology, University of Kentucky Chandler Medical Center, Lexington, KY 40536-0084, USA.
Abu Kwaik
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D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D010588:Phagosomes / Q000382:microbiology / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D014774:Virulence / Q000235:genetics