title: Angiotensinogen expression by rat epididymis: evidence for an intrinsic, angiotensin-generating system.
abstract: Previous studies have suggested the presence of an intrinsic renin-angiotensin system (RAS) in the rat epididymis with functions in epididymal activity and sperm maturation. In the present study, the localization and expression of angiotensinogen, the component of the RAS which is indispensable for intracellular angiotensin generation, were investigated by immunochemistry, hybridization histochemistry and by reverse-transcriptase polymerase chain reaction (RT-PCR). Western blot analysis of protein from the epididymis confirmed the presence of angiotensinogen with the expected molecular mass of about 60 kDa, in agreement with results from other tissues. Immunocytochemistry showed the regional localization of immunoreactivity for angiotensinogen in the rat epididymis. In situ hybridization histochemistry further demonstrated the expression of angiotensinogen mRNA by the epididymal epithelium in a region-specific manner along the length of the rat epididymis. RT-PCR confirmed that the rat epididymis expresses angiotensinogen mRNA. On the other hand, mRNA of renin was not detected in the rat epididymis using Northern blot and RT-PCR analyses. The present study strongly supports the existence of an intrinsic, angiotensin-generating system based on locally formed angiotensinogen as a precursor for angiotensin production. This epididymal RAS may have paracrine or autocrine roles in mediating the epididymal and sperm functions.
authors.affiliation: Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, NT, PR China. psleung@cuhk.edu.hk
authors.forename: P S
authors.initials: PS
authors.lastname: Leung
authors.forename: T P
authors.initials: TP
authors.lastname: Wong
authors.forename: C
authors.initials: C
authors.lastname: Sernia
mesh_terms: D000808:Angiotensinogen / Q000235:genetics*; D000809:Angiotensins / Q000235:genetics*; D000818:Animals; D004822:Epididymis / Q000378:metabolism*; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000235:genetics; D012084:Renin-Angiotensin System; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D000809:Angiotensins; D012333:RNA, Messenger; D000808:Angiotensinogen; D012083:Renin