title: A common breakpoint on 11q23 in carriers of the constitutional t(11;22) translocation.
abstract: Structural chromosomal rearrangements occur commonly in the general population. Individuals that carry a balanced translocation are at risk of having unbalanced offspring; therefore, the frequency of translocations in couples with recurrent spontaneous abortions is higher than that in the general population. The constitutional t(11;22) translocation is the most common recurrent non-Robertsonian translocation in humans and may serve as a model to determine the mechanism that causes recurrent meiotic translocations. We previously localized the t(11;22) translocation breakpoint to a region on 22q11 within a low-copy repeat, termed "LCR22." To define the breakpoint on 11q23 and to ascertain whether this region shares homology with LCR22 sequences, we performed haplotype analysis on patients with der(22) syndrome. We found that the breakpoint on 11q23 occurred between two genetic markers, D11S1340 and APOC3-tetra, both being present within a single bacterial-artificial-chromosome clone. To determine whether the breakpoint occurred within the same region among a larger set of carriers, we performed FISH mapping studies. The breakpoints were all within the same clone, suggesting that this region may harbor sequences that are prone to breakage. We narrowed the breakpoint interval, in both derivative chromosomes from two unrelated carriers, to a 190-bp, AT-rich repeat, which indicates that this repeat may mediate recombination events on chromosome 11. Interestingly, the LCR22s harbor AT-rich repeats, suggesting that this sequence motif may mediate recombination events in nonhomologous chromosomes during meiosis.
authors.affiliation: Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York, 10461, USA.
authors.forename: L
authors.initials: L
authors.lastname: Edelmann
authors.forename: E
authors.initials: E
authors.lastname: Spiteri
authors.forename: N
authors.initials: N
authors.lastname: McCain
authors.forename: R
authors.initials: R
authors.lastname: Goldberg
authors.forename: R K
authors.initials: RK
authors.lastname: Pandita
authors.forename: S
authors.initials: S
authors.lastname: Duong
authors.forename: J
authors.initials: J
authors.lastname: Fox
authors.forename: D
authors.initials: D
authors.lastname: Blumenthal
authors.forename: S R
authors.initials: SR
authors.lastname: Lalani
authors.forename: L G
authors.initials: LG
authors.lastname: Shaffer
authors.forename: B E
authors.initials: BE
authors.lastname: Morrow
mesh_terms: D000818:Animals; D001483:Base Sequence; D019457:Chromosome Breakage / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D004252:DNA Mutational Analysis; D005260:Female; D006239:Haplotypes / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D009630:Nondisjunction, Genetic; D020161:Physical Chromosome Mapping; D012727:Sex Characteristics; D013577:Syndrome; D014178:Translocation, Genetic / Q000235:genetics*