Xotx1 maternal transcripts are vegetally localized in Xenopus laevis oocytes.
Xotx1 is a Xenopus homeobox gene related to the Drosophila gene orthodenticle (otd). We previously reported that Xotx1 transcripts are already present in unfertilized egg. Here we report that maternal Xotx1 mRNA is vegetally localized during oogenesis. In stage II oocytes Xotx1 transcripts are localized within the mitochondrial cloud, in a perinuclear position; later on, they are translocated to the vegetal cortex within the mitochondrial cloud. We also observed that in stage III oocytes the expression domain of Wnt11 is contained within the one of Xotx1 while, at stage IV, the Xotx1 expression domain is contained within the one of Vg1.
Pannese
M
M
DIBIT, Scientific Institute H.S. Raffaele, Via Olgettina 60, 20132, Milan, Italy.
Cagliani
R
R
Pardini
C L
CL
Boncinelli
E
E
D000818:Animals; D005260:Female; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D051857:Otx Transcription Factors; D014157:Transcription Factors; D014158:Transcription, Genetic; D029867:Xenopus Proteins; D014982:Xenopus laevis
D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D051857:Otx Transcription Factors; C497657:Otx1 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins