Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes.
Production of two eicosanoids derived from lipoxygenase and cyclooxygenase activities: leukotriene B4 (LTB4) and prostaglandin E2 (PGE2), respectively, have been simultaneously determined in turbot (Scophthalmus maximus) blood leucocyte and kidney macrophage supernatants by a reverse phase high performance liquid chromatography (HPLC) system coupled with a Diode-Array detector. Levels of LTB4 after calcium ionophore challenge were 4.08 ng ml-1 in blood leukocyte supernatants and 0.25 ng ml-1 in kidney macrophage supernatants. The levels found for PGE2 were 428.23 and 606.67 ng ml-1 in blood leukocytes and kidney macrophage supernatants, respectively. When blood leukocytes were treated with the respective inhibitors for the enzymes implicated on the synthesis of both compounds an inhibition of 90.35% was observed for PGE2 and 76.44% for LTB4. The detection limit of the method was 0.15 ng ml-1 for LTB4 and 50 ng ml-1 for PGE2.
Tafalla
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C
Instituto de Investigaciones Marinas, CSIC, Vigo, Spain.
Medina
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I
Figueras
A
A
Novoa
B
B
D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D015777:Eicosanoids / Q000096:biosynthesis; D005412:Flatfishes / Q000097:blood*; D007213:Indomethacin / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D009637:Masoprocol / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D015777:Eicosanoids; D007975:Leukotriene B4; D009637:Masoprocol; D015232:Dinoprostone; D007213:Indomethacin