Dopamine enhances the phosphaturic effect of PTH during acute respiratory alkalosis.
The phosphaturic response to parathyroid hormone (PTH) is blunted during acute respiratory alkalosis. The objective of the present study was to determine the effect of dopamine on the blunted phosphaturic response to PTH during acute respiratory alkalosis. The phosphaturic response to PTH was determined in thyroparathyroidectomized (TPTX) normocapnic and respiratory alkalotic rats in the absence and presence of the infusion of exogenous dopamine (25 microg/kg/min) or of 3,4-dihydroxyphenylalanine (L-DOPA, 250 microg/kg/min) to increase endogenous dopamine synthesis. In normocapnic rats, PTH infusion (33 U/kg plus 1 U/kg/min) significantly increased the fractional excretion of phosphate (FE(Pi)), from 1.5%+/-0.5% to 28.4%+/-4.0%, (deltaFE(Pi) 26.9%+/-4.1%, n = 11, P<.05); in respiratory alkalotic rats, the increase was from 0.4%+/-0.1% to 11.4%+/-1.7% (deltaFE(Pi) 11.0%+/-1.8%, n = 13, P<.05). However, the phosphaturic response to PTH was attenuated in respiratory alkalotic rats (deltaFE(Pi) 26.9%+/-4.1% vs 11.0%+/-1.9%, P<.05). In normocapnic rats, in the presence of dopamine or L-DOPA infusions, PTH infusion significantly increased the FE(Pi) from 6.1%+/-2.3% to 33.4%+/-8.0% (deltaFE(Pi) 27.3%+/-7.0%, n = 5) and from 3.2%+/-0.6% to 32.5%+/-3.3% (deltaFE(Pi) 29.3%+/-3.2%, n = 7), respectively. In respiratory alkalotic rats, in the presence of dopamine infusion, PTH significantly increased the FE(Pi), from 0.6%+/-0.2% to 19.3%+/-3.3% (deltaFE(Pi) 18.7%+/-3.3%, n = 6); in the presence of L-DOPA infusion it increased from 1.0%+/-0.3% to 20.5%+/-2.8% (deltaFE(Pi) 19.5%+/-2.9%, n = 8, P<.05 as compared with PTH alone). Thus the phosphaturic effect of PTH that was attenuated in respiratory alkalotic rats was enhanced by stimulation of endogenous dopamine synthesis by the infusion of L-DOPA.
Berndt
T J
TJ
Department of Internal Medicine, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Tucker
R R
RR
Kent
P D
PD
Streiff
P C
PC
Tyce
G M
GM
Knox
F G
FG
D000208:Acute Disease; D000472:Alkalosis, Respiratory / Q000097:blood / Q000652:urine*; D000818:Animals; D001784:Blood Gas Analysis; D000242:Cyclic AMP / Q000652:urine; D004298:Dopamine / Q000096:biosynthesis / Q000494:pharmacology*; D004357:Drug Synergism; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000187:drug effects*; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology*; D016105:Parathyroidectomy; D010710:Phosphates / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013965:Thyroidectomy
D010281:Parathyroid Hormone; D010710:Phosphates; D007980:Levodopa; D000242:Cyclic AMP; D004298:Dopamine