title: Detection and effects on platelet function of anti-platelet antibody in mule foals with experimentally induced neonatal alloimmune thrombocytopenia.
abstract: Horse mares carrying mule foals were immunized during the last trimester of pregnancy with whole acid-citrate-dextrose-anticoagulated donkey blood to experimentally induce neonatal alloimmune thrombocytopenia. Thrombocytopenia occurred in the neonatal mule foals born to immunized horse mares within 24 hours after ingestion of their dams' colostrum. Mule foals born to mares not immunized with donkey blood did not develop thrombocytopenia. These findings suggest that antibodies may have been directed against a donkey platelet antigen present in the mule foals but not present in their dams. The objectives of this study were to determine whether anti-platelet antibody could be detected in mule foals with experimentally induced neonatal alloimmune thrombocytopenia, to identify any platelet proteins recognized by serum antibody in these foals, and to determine if platelet function was altered by sera from these mule foals. An indirect enzyme-linked immunosorbent assay demonstrated significantly higher absorption at 1:200 of platelet-bindable immunoglobulin G in serum from thrombocytopenic mule foals, compared with nonthrombocytopenic mule foals. Sera from thrombocytopenic and nonthrombocytopenic mule foals produced similar binding patterns in western immunoblots with donkey platelet proteins separated on sodium dodecyl sulfate polyacrylamide gels. Maximal platelet aggregation and relative slope of aggregation in response to collagen were significantly inhibited after incubation with sera from thrombocytopenic mule foals. These results suggest that mule foals with induced alloimmune thrombocytopenia have serum antibodies that bind to platelets and may compete with collagen binding sites to impair platelet aggregation.
authors.affiliation: Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, USA. santos_ramirez@ncsu.edu
authors.forename: S
authors.initials: S
authors.lastname: Ramirez
authors.forename: S D
authors.initials: SD
authors.lastname: Gaunt
authors.forename: J J
authors.initials: JJ
authors.lastname: McClure
authors.forename: J
authors.initials: J
authors.lastname: Oliver
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D016824:Antigens, Human Platelet / Q000276:immunology; D001327:Autoimmune Diseases / Q000151:congenital / Q000276:immunology / Q000662:veterinary*; D001667:Binding, Competitive; D001792:Blood Platelets / Q000276:immunology* / Q000502:physiology; D003094:Collagen; D018397:Equidae; D005260:Female; D006734:Horse Diseases / Q000151:congenital / Q000276:immunology*; D006736:Horses; D007114:Immunization / Q000662:veterinary; D008431:Maternal-Fetal Exchange; D010974:Platelet Aggregation; D011247:Pregnancy; D013921:Thrombocytopenia / Q000151:congenital / Q000276:immunology / Q000662:veterinary*
chemical_list: D000906:Antibodies; D016824:Antigens, Human Platelet; D003094:Collagen