A Scj1p homolog and folding catalysts present in dog pancreas microsomes.
Dog pancreas microsomes represent the key components of the established model system for the analysis of protein transport into the mammalian endoplasmic reticulum. More recently, these microsomes were also employed in cell-free systems which address questions related to protein folding and protein degradation in the mammalian endoplasmic reticulum. In order to get at a complete picture of these undoubtedly related processes in the in vitro system we need to know all the proteins we are dealing with, and their respective stoichiometries. Here we give a progress report on our attempts to identify and to quantify the soluble molecular chaperones and folding catalysts which are present in the lumen of dog pancreas microsomes. Eventually, we will need to know how the in vitro system compares with the situation in intact pancreatic cells as well as in other cells.
Bies
C
C
Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Homburg, Germany.
Guth
S
S
Janoschek
K
K
Nastainczyk
W
W
Volkmer
J
J
Zimmermann
R
R
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans; D004285:Dogs; D004926:Escherichia coli; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism*; D017510:Protein Folding; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C402945:ERj3 protein, Canis familiaris; D005656:Fungal Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C060153:KAR2 protein, yeast; D018832:Molecular Chaperones; C067634:SCJ1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins