NMR study of pradimicin derivative BMY-28864 and its interaction with calcium ions in D2O.
The dynamic structure of the antifungal antibiotic pradimicin BMY-28864 in D2O and its interaction with calcium ions were analyzed using one- and two-dimensional 1H nuclear magnetic resonance (NMR). Spectra indicate extensive self-association of molecules in the solution. Two-component spectra were observed simultaneously in a very dilute solution, suggesting equilibrium of two aggregative states. The addition of CaCl2 caused a number of changes in NMR spectra. Therefore we concluded that pradimicin BMY-28864 could form a complex with the Ca2+ ion, causing a movement of the equilibrium. The position of the bound calcium ion is determined indirectly by observing how the NMR shift affects protons that are close to the binding site. The stoichiometry of Ca2+ ion to the Pradimicin molecule for the Ca(2+)-saturated complex is verified to be 1:2. Signal broadening and changes in chemical shift in the 1H NMR spectroscopy of BMY-28864 are assumed to be related to changes in the molecular aggregate conformation.
Hu
M
M
National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Ishizuka
Y
Y
Igarashi
Y
Y
Oki
T
T
Nakanishi
H
H
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D003903:Deuterium; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D012996:Solutions; D013696:Temperature; D000095643:Pradimicins and Benanomicins
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D002118:Calcium; D002413:Cations, Divalent; D003903:Deuterium; D012996:Solutions; C058036:pradimicin A; D000095643:Pradimicins and Benanomicins; C067714:N,N-dimethylpradimicin FA-2