Lyso-PAF analogues and lysophosphatidylcholines from the marine sponge Spirastrella abata as inhibitors of cholesterol biosynthesis.
A series of phospholipids, including previously undescribed compounds 4-7, were isolated by a bioactivity-guided fractionation from the marine sponge Spirastrella abata as inhibitors of cholesterol biosynthesis in human liver cells. These compounds were identified as lyso-PAF analogues (1-5) and lysophosphatidylcholines (6, 7) based on NMR and MS analyses. Compounds 1-7 specifically blocked the conversion of lanosterol into cholesterol in the Chang liver cell.
Shin
B A
BA
College of Pharmacy, Pusan National University, Pusan 609-735, The Research Institute of Medical Sciences, Chonnam National University, Kwangju, College of Pharmacy, Chonnam National University, Kwangju, Korea Basic Science Institute, Taejon, and Dep.
Kim
Y R
YR
Lee
I S
IS
Sung
C K
CK
Hong
J
J
Sim
C J
CJ
Im
K S
KS
Jung
J H
JH
D000818:Animals; D000924:Anticholesteremic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003864:Depression, Chemical; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D018836:Inflammation Mediators / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007810:Lanosterol / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008148:Lovastatin / Q000031:analogs & derivatives / Q000494:pharmacology; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry / Q000378:metabolism*
D000924:Anticholesteremic Agents; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D018836:Inflammation Mediators; D008244:Lysophosphatidylcholines; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D007810:Lanosterol; C012258:mevastatin; D002784:Cholesterol; D008148:Lovastatin