Reduction of sialic acid O-acetylation in human colonic mucins in the adenoma-carcinoma sequence.
The oligo-O-acetylation of sialic acids found in normal colonic mucins is greatly reduced in colorectal cancer. Mucins prepared from cancer tissue in adenocarcinoma showed this reduction, while normal O-acetylation was detected in resection margin and control cases and total mucin sialic acid content was significantly decreased in cancer vs. control samples. A reduction of the O-acetyl transferase activity catalysing the O-acetylation reaction was also found. A series of cultured human colorectal cell lines derived from the same premalignant adenomatous line, and representative of the adenoma-carcinoma sequence were examined and revealed a depletion of oligo-O-acetylation in the original diploid premalignant line, re-expression in a further premalignant line and reduction in malignant mucinous and adenocarcinoma cell lines. Reduction of sialic acid O-acetylation appears as an early event in the process of malignant transformation in human colorectal cancer.
Corfield
A P
AP
University Department of Medicine, Bristol Royal Infirmary, UK. Tony.Corfield@bristol.ac.uk
Myerscough
N
N
Warren
B F
BF
Durdey
P
P
Paraskeva
C
C
Schauer
R
R
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D000230:Adenocarcinoma / Q000201:enzymology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D009077:Mucins / Q000737:chemistry / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000939:Epitopes; D009077:Mucins; D000123:Acetyltransferases; D019158:N-Acetylneuraminic Acid