title: Automated latex agglutination and ELISA testing yield equivalent D-dimer results in patients with recent myocardial infarction. THROMBO Research Investigators.
abstract: Our previous prospective study of post-infarction patients described a strong and significant association of increased plasma D-dimer concentrations in those who experienced a subsequent coronary death or non-fatal myocardial infarction. In the present study, we compare results on stored plasma obtained two months after the index myocardial infarction from 1,038 patients of this trial, using a simple automated latex agglutination (LA) assay in parallel with the standard ELISA test. Results show a somewhat higher mean value for the LA assay (702+/-1092 vs. 638+/-986 ng/ml, p = 0.0002), a strong linear correlation of the two assays (r = 0.86) and 88% agreement for values below 500 ng/ml by the ELISA test. D-dimer concentrations determined by each assay were highly correlated in patients with subsequent coronary artery events (p = 0.93) and quartile values for both the LA and ELISA were equally predictive of such events (p = 0.003 and p = 0.001, respectively). This is the first demonstration that a latex agglutination assay for D-dimer can be used to assess the prognostic risk of recurrent coronary thrombotic disease after myocardial infarction
authors.affiliation: Orthopaedic Hospital/UCLA School of Medicine, Los Angeles, CA, USA. vmarder@laoh.ucla.edu
authors.forename: V J
authors.initials: VJ
authors.lastname: Marder
authors.forename: W
authors.initials: W
authors.lastname: Zareba
authors.forename: J T
authors.initials: JT
authors.lastname: Horan
authors.forename: A J
authors.initials: AJ
authors.lastname: Moss
authors.forename: J J
authors.initials: JJ
authors.lastname: Kanouse
mesh_terms: D000328:Adult; D001331:Automation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D009203:Myocardial Infarction / Q000097:blood*; D011379:Prognosis; D012008:Recurrence; D012189:Retrospective Studies
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D