title: Tolerance to maternal immunoglobulins: resilience of the specific T cell repertoire in spite of long-lasting perturbations.
abstract: T cell tolerance is established and maintained through various mechanisms, the critical component being the persistence of the specific Ag. However, at the molecular level, the nature of the recovering TCR repertoire following breakdown of tolerance is unknown. We address this important question by following kappa light chain constant region (C kappa)-specific CD4+ T cells of kappa light chain knock-out (kappa-/-) mice born to kappa+/- mothers. These cells, which were in contact with maternal kappa+ Igs from early ontogeny until weaning, were strongly tolerized. Tolerance was reversible and waned with the disappearance of peptide C kappa 134-148 presentation in lymphoid organs, including the thymus. Whereas three specific V beta-J beta rearrangements emerged in the peptide C kappa 134-148-specific CD4+ T cell response of all regular kappa-/- mice, soon after breakdown of tolerance only one of these rearrangements was detected. The two others displayed a significant delay in reappearance and were still rare at 26 wk of age, while the control proliferative response had already recovered 3 mo earlier. At 52 wk of age, a complete recovery of the three canonical V beta-J beta rearrangements was observed. Thus, although profoundly perturbed for several months, the T cell repertoire returns to equilibrium, highlighting the resilient nature of this system.
authors.affiliation: Unité d'Immunochimie Analytique (URA Centre National de la Recherche Scientifique 1961, Université Pierre et Marie Curie), Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Faure
authors.forename: S
authors.initials: S
authors.lastname: Calbo
authors.forename: J
authors.initials: J
authors.lastname: Kanellopoulos
authors.forename: A M
authors.initials: AM
authors.lastname: Drapier
authors.forename: P A
authors.initials: PA
authors.lastname: Cazenave
authors.forename: D
authors.initials: D
authors.lastname: Rueff-Juy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D007108:Immune Tolerance / Q000235:genetics; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008213:Lymphocyte Activation / Q000235:genetics; D008297:Male; D008431:Maternal-Fetal Exchange / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*
chemical_list: D018984:Epitopes, T-Lymphocyte; D007127:Immunoglobulin Constant Regions; D007145:Immunoglobulin kappa-Chains; D016693:Receptors, Antigen, T-Cell, alpha-beta