Insight into the molecular evolution of two tropinone reductases.
In tropane alkaloid biosynthesis, two tropinone reductases produce different stereoisomers from a common substrate, tropinone. The two enzymes share 64% of identical amino acids, and highly homologous proteins with variable substrate-binding residues have also been found in tropane alkaloid non-producing species. This exemplifies a simple evolutionary process that plants have taken to acquire a new secondary metabolic pathway.
Nakajima
K
K
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan.
Oshita
Y
Y
Yamada
Y
Y
Hashimoto
T
T
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D016208:Databases, Factual; D019143:Evolution, Molecular; D008956:Models, Chemical; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D018076:DNA, Complementary; D010940:Plant Proteins; D000429:Alcohol Oxidoreductases; C083753:tropinone reductase