title: Global transposon mutagenesis and a minimal Mycoplasma genome.
abstract: Mycoplasma genitalium with 517 genes has the smallest gene complement of any independently replicating cell so far identified. Global transposon mutagenesis was used to identify nonessential genes in an effort to learn whether the naturally occurring gene complement is a true minimal genome under laboratory growth conditions. The positions of 2209 transposon insertions in the completely sequenced genomes of M. genitalium and its close relative M. pneumoniae were determined by sequencing across the junction of the transposon and the genomic DNA. These junctions defined 1354 distinct sites of insertion that were not lethal. The analysis suggests that 265 to 350 of the 480 protein-coding genes of M. genitalium are essential under laboratory growth conditions, including about 100 genes of unknown function.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Hutchison
authors.forename: S N
authors.initials: SN
authors.lastname: Peterson
authors.forename: S R
authors.initials: SR
authors.lastname: Gill
authors.forename: R T
authors.initials: RT
authors.lastname: Cline
authors.forename: O
authors.initials: O
authors.lastname: White
authors.forename: C M
authors.initials: CM
authors.lastname: Fraser
authors.forename: H O
authors.initials: HO
authors.lastname: Smith
authors.forename: J C
authors.initials: JC
authors.lastname: Venter
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D004258:DNA Polymerase III / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics; D004251:DNA Transposable Elements; D020043:Genes, Essential; D016680:Genome, Bacterial; D006019:Glycolysis / Q000235:genetics; D008074:Lipoproteins / Q000235:genetics; D016254:Mutagenesis, Insertional; D009174:Mycoplasma / Q000235:genetics* / Q000378:metabolism; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008074:Lipoproteins; D012269:Ribosomal Proteins; D004258:DNA Polymerase III; D000604:Amino Acyl-tRNA Synthetases