Opsonization of Toxoplasma gondii tachyzoites with nonspecific immunoglobulins promotes their phagocytosis by macrophages and inhibits their proliferation in nonphagocytic cells in tissue culture.
We have recently shown that Toxoplasma gondii tachyzoites grown in in vitro culture can bind unspecific immunoglobulin (Ig) through their Fc moiety. We show now that Fc receptors are also present on T. gondii within the host animal, and that intraperitoneal parasites in immunocompetent mice are saturated with unspecific Ig. We have also investigated the effect of the parasite's Fc receptor on the interaction of tachyzoites with mammalian cells, using the Vero cell line as a model for nonphagocytic host cells and murine peritoneal macrophages in primary culture as a model for phagocytic cells. Coating of tachyzoites with parasite-unrelated Ig did not enhance their invasive capacity in either target cell type, but slightly decreased the parasite proliferation. Moreover, phagocytosis by macrophages was increased by approximately 50% when parasites were coated with unspecific Ig. These results indicate that the Fc receptor on T. gondii affects the balance between invasion and phagocytosis in a way that is detrimental to the parasites.
Vercammen
M
M
Laboratorium voor Toxoplasmose, Pasteur Instituut, Engelandstraat 642, 1180 Brussels, Belgium.
Scorza
T
T
El Bouhdidi
A
A
Van Beeck
K
K
Carlier
Y
Y
Dubremetz
J F
JF
Verschueren
H
H
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000378:metabolism* / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D010587:Phagocytosis; D011961:Receptors, Fc / Q000276:immunology / Q000378:metabolism*; D014122:Toxoplasma / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*; D014709:Vero Cells / Q000469:parasitology
D007136:Immunoglobulins; D011961:Receptors, Fc