Investigating the relaxation rate, following diazo-2 photolysis, of a skinned trabecular preparation from guinea-pig hypertrophied left ventricle.
Cardiac hypertrophy in the guinea-pig is not accompanied by a large shift in the expression of the predominant isoform of myosin in the left ventricle; however, in this species, thin filament proteins do change. We examined the relaxation, following laser flash photolysis of the photolabile caged Ca2+ chelator diazo-2, of a skinned trabecular preparation from the left ventricle of guinea-pigs that had undergone abdominal aortic banding. Sham-operated animals were used as controls; no guinea-pigs showed any signs of heart failure. We report that mild cardiac hypertrophy does not affect the relaxation rate of Triton-skinned trabeculae from the guinea-pig. However, there was a 35% reduction in the maximum force generated by trabeculae from the left ventricle of the abdominal aortic-banded animals. Additionally, alterations in key troponin subunits occur in the left ventricle of guinea-pigs with mild hypertrophy. We conclude that the thin filament protein changes do not influence trabecular relaxation rates, even though they probably affect maximal force generation. The cellular membrane systems of the intact guinea-pig heart, which were not a factor in this present study, appear to have an important role in the altered cardiac relaxation rates seen in hypertrophy.
Johns
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EC
University Laboratory of Physiology, Oxford, UK.
Ryder
K O
KO
Hodson
E A
EA
Hart
G
G
Mulligan
I P
IP
Lipscomb
S
S
Ashley
C C
CC
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D003979:Diazonium Compounds; D006168:Guinea Pigs / Q000502:physiology*; D006321:Heart / Q000503:physiopathology; D006652:Histological Techniques; D017379:Hypertrophy, Left Ventricular / Q000503:physiopathology*; D009200:Myocardial Contraction / Q000502:physiology*; D010642:Phenoxyacetates; D010782:Photolysis
D002614:Chelating Agents; D003979:Diazonium Compounds; D010642:Phenoxyacetates; C063685:diazo-2