title: Determination of emamectin benzoate in medicated fish feed.
abstract: A method was developed to quantitate emamectin benzoate in fish feed at levels between 5 and 15 ppm. The active ingredient is extracted from 20 g medicated feed into aqueous-methanolic solvent by overnight shaking. A solid-phase extraction procedure using a 2 g C18 cartridge is then used to concentrate the active residue and remove interfering matrix components. The extracted drug and internal standard are eluted from the cartridge, evaporated to dryness, and reconstituted in methanol. A control feed sample and fortified control working standard are simultaneously prepared. Remaining interferences and sample analysis are further separated on a gradient liquid chromatographic system. Recovery of emamectin benzoate from fortified feeds ranged from 97 to 100%, with a coefficient of variation (CV) of 1.2%. Determination of emamectin benzoate in medicated feeds resulted in CVs ranging from 2.3 to 4.2% and recoveries of 88 to 98% of label claim.
authors.affiliation: Schering-Plough Research Institute, Analytical Development, Kenilworth, NJ 07033, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Farer
authors.forename: J
authors.initials: J
authors.lastname: Hayes
authors.forename: J
authors.initials: J
authors.lastname: Rosen
authors.forename: P
authors.initials: P
authors.lastname: Knight
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D005399:Fishes; D005503:Food Additives / Q000032:analysis; D007306:Insecticides / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D005503:Food Additives; D007306:Insecticides; D007559:Ivermectin; C108024:emamectin benzoate