title: Errors in estimating lung liquid volume in fetal lambs when using radiolabeled serum albumin and blue dextran.
abstract: Fetal lung liquid volume is usually determined by using radio-iodinated serum albumin (RISA) or blue dextran (BD) as volume tracers. We tested the reliability of both tracers at 124 (G124) and 142 days of gestation (G142; term = G147) when the labels were employed simultaneously. We measured the proportion of label bound reversibly to the lung, or apparently lost from the lung compartment, by washing out the lung with saline and 5% albumin. At G124, volume estimates with the two labels were similar. At G142, the volume estimate with BD (36.3 +/- 8.7 ml/kg of body wt) was higher (P < 0. 05) than with RISA (22.3 +/- 3.5 ml/kg). This difference resulted from reversible binding of BD, because 5% albumin washout released 38.5 +/- 4.0% of the BD added at the start of the experiment but a lesser amount of RISA (9.8 +/- 0.7%; P < 0.05). At G142, when RISA was used alone, its reversible binding was 1.3 +/- 0.2%. Background absorbance increased during experiments, giving rise to an apparent increase in BD concentration. We conclude that RISA is an effective tracer for lung liquid volume determination in the fetal lamb, whereas our findings of substantial epithelial binding of BD and large changes in background absorbance demonstrate that, under the conditions of our experiments, BD is a poor tracer close to term.
authors.affiliation: Ritchie Centre for Baby Health Research, Institute of Reproduction and Development, Monash Medical Centre, Clayton, Victoria 3168, Australia.
authors.forename: R E
authors.initials: RE
authors.lastname: Pfister
authors.forename: C A
authors.initials: CA
authors.lastname: Ramsden
authors.forename: H L
authors.initials: HL
authors.lastname: Neil
authors.forename: M A
authors.initials: MA
authors.lastname: Kyriakides
authors.forename: P J
authors.initials: PJ
authors.lastname: Berger
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D004396:Coloring Agents; D003911:Dextrans; D004338:Drug Combinations; D004347:Drug Interactions; D005333:Fetus / Q000378:metabolism; D008168:Lung / Q000196:embryology*; D019275:Radiopharmaceuticals; D012711:Serum Albumin, Radio-Iodinated; D012756:Sheep / Q000196:embryology
chemical_list: D004396:Coloring Agents; D003911:Dextrans; D004338:Drug Combinations; D019275:Radiopharmaceuticals; D012711:Serum Albumin, Radio-Iodinated; C017080:blue dextran