Evidence for an ependymoma tumour suppressor gene in chromosome region 22pter-22q11.2.
Ependymomas are glial tumours of the brain and spinal cord. The most frequent genetic change in sporadic ependymoma is monosomy 22, suggesting the presence of an ependymoma tumour suppressor gene on that chromosome. Clustering of ependymomas has been reported to occur in some families. From an earlier study in a family in which four cousins developed an ependymoma, we concluded that an ependymoma-susceptibility gene, which is not the NF2 gene in 22q12, might be located on chromosome 22. To localize that gene, we performed a segregation analysis with chromosome 22 markers in this family. This analysis revealed that the susceptibility gene may be located proximal to marker D22S941 in 22pter-22q11.2. Comparative genomic hybridization showed that monosomy 22 was the sole detectable genetic aberration in the tumour of one of the patients. Loss of heterozygosity studies in that tumour revealed that, in accordance to Knudson's two-hit theory of tumorigenesis, the lost chromosome 22 originated from the parent presumed to have contributed the wild-type allele of the susceptibility gene. Thus, our segregation and tumour studies collectively indicate that an ependymoma tumour suppressor gene may be present in region 22pter-22q11.2.
Hulsebos
T J
TJ
Department of Human Genetics, University of Amsterdam, The Netherlands.
Oskam
N T
NT
Bijleveld
E H
EH
Westerveld
A
A
Hermsen
M A
MA
van den Ouweland
A M
AM
Hamel
B C
BC
Tijssen
C C
CC
D016543:Central Nervous System Neoplasms / Q000235:genetics*; D020090:Chromosome Segregation; D002892:Chromosomes, Human, Pair 22; D020732:Cytogenetic Analysis; D004806:Ependymoma / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D020022:Genetic Predisposition to Disease; D006801:Humans; D009693:Nucleic Acid Hybridization; D010375:Pedigree