Characterization of enzymatically induced degradation of articular cartilage using high frequency ultrasound.
Ultrasound may provide a quantitative technique for the characterization of cartilage changes typical of early osteoarthrosis. In this study, specific changes in bovine articular cartilage were induced using collagenase and chondroitinase ABC, enzymes that selectively degrade collagen fibril network and digest proteoglycans, respectively. Changes in cartilage structure and properties were quantified using high frequency ultrasound, microscopic analyses and mechanical indentation tests. The ultrasound reflection coefficient of the physiological saline-cartilage interface (R1) decreased significantly (-96.4%, p < 0.01) in the collagenase digested cartilage compared to controls. Also a significantly lower ultrasound velocity (-6.2%, p < 0.01) was revealed after collagenase digestion. After chondroitinase ABC digestion, a new acoustic interface at the depth of the enzyme penetration front was detected. Cartilage thickness, as determined with ultrasound, showed a high, linear correlation (R = 0.943, n = 60, average difference 0.073 mm (4.0%)) with the thickness measured by the needle-probe method. Both enzymes induced a significant decrease in the Young's modulus of cartilage (p < 0.01). Our results indicate that high frequency ultrasound provides a sensitive technique for the analysis of cartilage structure and properties. Possibly ultrasound may be utilized in vivo as a quantitative probe during arthroscopy.
Töyräs
J
J
Department of Clinical Physiology, Kuopio University Hospital, Finland. juha.toyras@uku.fi
Rieppo
J
J
Nieminen
M T
MT
Helminen
H J
HJ
Jurvelin
J S
JS
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000000981:diagnostic imaging* / Q000502:physiology; D002417:Cattle; D019765:Chondroitin ABC Lyase; D003094:Collagen / Q000378:metabolism; D017364:Collagenases; D011509:Proteoglycans / Q000378:metabolism; D014463:Ultrasonography / Q000295:instrumentation / Q000379:methods
D011509:Proteoglycans; D003094:Collagen; D017364:Collagenases; D019765:Chondroitin ABC Lyase