Three novel mutations at serine 314 in the thyroid hormone beta receptor differentially impair ligand binding in the syndrome of resistance to thyroid hormone.
The syndrome of resistance to thyroid hormone is associated with diverse mutations in the ligand-binding domain of the thyroid hormone beta receptor, localizing to three clusters around the hormone binding cavity. Here, we report three novel resistance to thyroid hormone mutations (S314C, S314F, and S314Y), due to different nucleotide substitutions in the same codon, occurring in six separate families. Functional characterization of these mutant receptors showed marked differences in their properties. S314F and S314Y receptor mutants exhibited significant transcriptional impairment in keeping with negligible ligand binding and were potent dominant negative inhibitors of wild-type receptor action. In contrast, the S314C mutant bound ligand with reduced affinity, such that its functional impairment and dominant negative activity manifest at low concentrations of thyroid hormone, but are more reversible at higher T3 concentrations. The degree of functional impairment of mutant receptors in vitro may correlate with the magnitude of thyroid dysfunction in vivo. Modelling these mutations using the crystal structure of thyroid hormone receptor beta shows why ligand binding is perturbed and why the phenylalanine/tyrosine mutations are more deleterious than cysteine.
Gurnell
M
M
Department of Medicine, University of Cambridge, Addenbrigge, Addenbrooke's Hospital, United Kingdom.
Rajanayagam
O
O
Agostini
M
M
Clifton-Bligh
R J
RJ
Wang
T
T
Zelissen
P M
PM
van der Horst
F
F
van de Wiel
A
A
Macchia
E
E
Pinchera
A
A
Schwabe
J W
JW
Chatterjee
V K
VK
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003460:Crystallization; D004247:DNA / Q000378:metabolism; D019281:Dimerization; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation; D011988:Receptors, Thyroid Hormone / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012694:Serine / Q000235:genetics*; D018382:Thyroid Hormone Resistance Syndrome / Q000235:genetics*; D014162:Transfection; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology
D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D012694:Serine; D004247:DNA