[Use of the immunoenzyme test ELISA-NS3 to distinguish horses infected by African horsesickness virus from vaccinated horses].
A vaccination protocol involving three horses, with five repeated injections of inactivated serotype 4 African horse sickness virus, was undertaken to determine a possible threshold for the appearance of antibodies against the non-structural protein NS3. Using an indirect enzyme-linked immunosorbent assay, with the recombinant NS3 protein as an antigen, the authors detected a response to NS3 as of the second injection for the first horse and after four injections for the second horse. No response to NS3 was detected for the third horse. The results show that the inactivated vaccine is insufficiently purified to eliminate the non-structural protein NS3. Therefore using the NS3 protein as a marker did not enable differentiation between vaccinated and infected horses.
Idrissi Bougrine
S
S
Institut agronomique et vétérinaire Hassan II, Département de microbiologie, immunologie et maladies contagieuses, Rabat-Instituts, Maroc.
Fassi Fihri
O
O
el Harrak
M
M
Fassi Fehri
M M
MM
D000355:African Horse Sickness / Q000175:diagnosis / Q000276:immunology* / Q000517:prevention & control; D000356:African Horse Sickness Virus / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006736:Horses; D009500:Neutralization Tests / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D015164:Vaccines, Inactivated; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines
Utilisation de l'épreuve immuno-enzymatique ELISA-NS3 pour différencier les chevaux infectés par le virus de la peste équine des chevaux vaccinés.