Suppression of metastasis by tissue inhibitor of metalloproteinase-1 in a newly established human oral squamous cell carcinoma cell line.
We have established a highly-metastatic cell line (designated as HNOS) and a non-metastatic cell line (designated as SAT) derived from human oral cavity squamous cell carcinoma (SCC). Both lines were transplantable in nude mice. The invasive activity through Matrigel-coated membrane of HNOS cells was also higher than that of SAT cells. mRNA of TIMP-1 was expressed in SAT cells but not in HNOS cells. Metastatic and invasive abilities were suppressed by the overexpression of TIMP-1 in HNOS cells. These results suggest that TIMP-1 may have an important role in inhibiting invasion and metastasis of human oral cavity SCC.
Nii
M
M
Department of Molecular Oral Medicine and Maxillofacial Surgery I, Hiroshima University, School of Dentistry, Minami-ku, Hiroshima 734-8553, Japan.
Kayada
Y
Y
Yoshiga
K
K
Takada
K
K
Okamoto
T
T
Yanagihara
K
K
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000517:prevention & control* / Q000556:secondary; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000473:pathology / Q000517:prevention & control*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000517:prevention & control; D009368:Neoplasm Transplantation; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use*; D014162:Transfection
D018076:DNA, Complementary; D019715:Tissue Inhibitor of Metalloproteinase-1; D020781:Matrix Metalloproteinase 1