Separation, identification and determination of sanguinarine in argemone and other adulterated edible oils by reversed-phase high-performance liquid chromatography.
A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the separation and determination of sanguinarine in argemone and other edible oils has been developed. The separation has been achieved on a C18 column with CH3OH-CH3CN-tetrahydrofuran-water as mobile phase using diode array detection at 280 nm. The minimum detection limit of sanguinarine in the adulterated edible oils is 5 microg/g.
Husain
S
S
Analytical Chemistry and Environmental Science Division, Indian Institute of Chemical Technology, Hyderabad.
Narsimha
R
R
Rao
R N
RN
D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D053119:Benzophenanthridines; D002851:Chromatography, High Pressure Liquid; D005663:Furans; D007202:Indicators and Reagents; D007546:Isoquinolines; D009149:Mustard Plant / Q000737:chemistry; D010938:Plant Oils / Q000032:analysis*; D010946:Plants, Medicinal; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D053119:Benzophenanthridines; D005663:Furans; D007202:Indicators and Reagents; D007546:Isoquinolines; D010938:Plant Oils; D012997:Solvents; C030610:argemone oil; C018674:tetrahydrofuran; C005705:sanguinarine