title: Dynamics of retinal waves are controlled by cyclic AMP.
abstract: Waves of spontaneous activity sweep across the developing mammalian retina and influence the pattern of central connections made by ganglion cell axons. These waves are driven by synaptic input from amacrine cells. We show that cholinergic synaptic transmission during waves is not blocked by TTX, indicating that release from starburst amacrine cells is independent of sodium action potentials. The spatiotemporal properties of the waves are regulated by endogenous release of adenosine, which sets intracellular cAMP levels through activation of A2 receptors present on developing amacrine and ganglion cells. Increasing cAMP levels increase the size, speed, and frequency of the waves. Conversely, inhibiting adenylate cyclase or PKA prevents wave activity. Together, these results imply a novel mechanism in which levels of cAMP within an immature retinal circuit regulate the precise spatial and temporal patterns of spontaneous neural activity.
authors.affiliation: Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA. stell@uclink4.berkeley.edu
authors.forename: D
authors.initials: D
authors.lastname: Stellwagen
authors.forename: C J
authors.initials: CJ
authors.lastname: Shatz
authors.forename: M B
authors.initials: MB
authors.lastname: Feller
mesh_terms: D000241:Adenosine / Q000502:physiology; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism / Q000502:physiology*; D005289:Ferrets; D066298:In Vitro Techniques; D051379:Mice; D011963:Receptors, GABA-A / Q000502:physiology; D018047:Receptors, Purinergic P1 / Q000502:physiology; D012160:Retina / Q000502:physiology*
chemical_list: D011963:Receptors, GABA-A; D018047:Receptors, Purinergic P1; D000242:Cyclic AMP; D000241:Adenosine