title: Glutamate induces rapid upregulation of astrocyte glutamate transport and cell-surface expression of GLAST.
abstract: Glutamate transporters clear glutamate from the extracellular space by high-affinity binding and uptake. Factors that regulate glutamate transporter expression and activity can thereby influence excitatory neurotransmission. Transporter function in GABAergic and other systems has been shown to be regulated by transporter substrates. Here, glutamate regulation of glutamate transport was studied using primary murine astrocyte cultures that express the GLAST (EAAT1) and GLT-1 (EAAT2) transporter subtypes. Glutamate was found to stimulate glutamate transport capacity (V(max)) in a dose- and time-dependent manner. The maximal increase was 100%, with an ED(50) of 40 microM glutamate and with onset beginning approximately 15 min after onset of glutamate exposure. The uptake stimulation was reproduced by D-aspartate, which is also a transporter substrate, but not by nontransported glutamate receptor agonists. Moreover, glutamate incubation did not stimulate transport when performed in a sodium-free medium, suggesting that the stimulatory effect of glutamate is triggered by increased transporter activity rather than receptor activation. Treatment with the actin-disrupting agents cytochalasin B or cytochalasin D prevented the glutamate-induced increase in glutamate uptake. Biotinylation labeling of membrane surface proteins showed that glutamate incubation produced an increase in GLAST expression at the astrocyte cell surface. These results suggest that cell-surface expression of GLAST can be rapidly regulated by glutamate through a process triggered by GLAST activity and involving the actin cytoskeleton. This feedback loop provides a mechanism by which changes in extracellular glutamate concentrations could rapidly modulate astrocyte glutamate transport capacity.
authors.affiliation: Department of Neurology, University of California, San Francisco, USA.
authors.forename: S
authors.initials: S
authors.lastname: Duan
authors.forename: C M
authors.initials: CM
authors.lastname: Anderson
authors.forename: B A
authors.initials: BA
authors.lastname: Stein
authors.forename: R A
authors.initials: RA
authors.lastname: Swanson
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism* / Q000502:physiology; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D027342:Excitatory Amino Acid Transporter 2; D018698:Glutamic Acid / Q000493:pharmacokinetics / Q000494:pharmacology*; D051379:Mice; D017470:Receptors, Glutamate / Q000502:physiology; D017981:Receptors, Neurotransmitter / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D027342:Excitatory Amino Acid Transporter 2; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D015638:Cytochalasin D; D003571:Cytochalasin B; D018698:Glutamic Acid