Efficiency studies in nonaqueous capillary electrophoresis.
Nonaqueous capillary electrophoresis (NACE) is a relatively new area with several advantages that include enhanced efficiency and improved detection sensitivity. The goal of this study was to investigate the influence of NACE compared to aqueous CE on the separation efficiency of oligosaccharides. The applied voltage and buffer concentration were optimized for the aqueous and nonaqueous buffer media to minimize the band broadening effects of Joule heating and electrophoretic dispersion. At the optimized conditions a 1.5-fold enhancement in efficiency was obtained with the nonaqueous buffer medium.
Ward
V L
VL
Department of Chemistry, North Carolina State University, Raleigh 27695-8204, USA.
Khaledi
M G
MG
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005559:Formamides; D007202:Indicators and Reagents; D009844:Oligosaccharides / Q000302:isolation & purification; D011786:Quality Control; D012680:Sensitivity and Specificity; D014867:Water
D002021:Buffers; D005559:Formamides; D007202:Indicators and Reagents; D009844:Oligosaccharides; D014867:Water; C031066:formamide