Receptor tyrosine kinase tie 1 mRNA is upregulated on cerebral microvessels after embolic middle cerebral artery occlusion in rat.
Tie 1 is an endothelial specific transmembrane receptor tyrosine kinase and may be required during angiogenesis. Using in situ hybridization, we measured tie 1 mRNA in ischemic brain (n=15). Rats were subjected to middle cerebral artery (MCA) occlusion by a single fibrin rich clot. Expression of tie 1 was not detected in non ischemic brain. Cerebral microvessels expressed tie 1 in the ischemic lesion as early as 2 h after MCA occlusion. The number of microvessels containing tie 1 mRNA decreased in the ischemic lesion at 8 h after MCA occlusion. However, expression of tie 1 increased on microvessels at 24 h and 14 days after ischemia and tie 1 was primarily localized to the microvessels bordering pan necrotic tissue. Ninety-seven percent of cerebral vessels which expressed tie 1 mRNA had diameters of 3.7+/-0.17 microm. Our findings suggest a role for tie 1 in cerebral microvascular remodeling after embolic stroke.
Zhang
Z G
ZG
Department of Neurology, Henry Ford Health Science Center, Detroit, MI 48202, USA.
Chopp
M
M
Lu
D
D
Wayne
T
T
Zhang
R L
RL
Morris
D
D
D000818:Animals; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism*; D008297:Male; D008833:Microcirculation; D018919:Neovascularization, Physiologic / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D042810:Receptor, TIE-1; D011956:Receptors, Cell Surface / Q000378:metabolism*; D042764:Receptors, TIE
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D020794:Receptor Protein-Tyrosine Kinases; D042810:Receptor, TIE-1; D042764:Receptors, TIE