Isolation and characterization of the hypoxia-inducible factor 1beta in Drosophila melanogaster.
The hypoxia-inducible factor 1 (HIF-1), a heterodimer composed of alpha and beta subunits, plays an important role in the cellular response to O(2) deprivation. In this paper, Drosophila HIF-1beta (dHIF-1beta) homolog is cloned and characterized. Further, Northern analyses showed that dHIF-1alpha and dHIF-1beta expressed their highest level at an embryonic stage. From the pupal stage on, their expression was sharply reduced and maintained at a steady level. Anoxia treatment up-regulated the expression of the both alpha and beta subunits. Over-expression of dHIF-1alpha in transgenic embryos resulted in embryonic lethality, while over-expression of dHIF-1beta significantly prolonged fly recovery time from a 5-min anoxic stupor. The cloning and characterization dHIF-1beta reported in this paper provide a framework for further genetic dissection of the HIF-1 complex in its role in the cellular or tissue response to O(2) deprivation.
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Department of Pediatrics (Section of Respiratory Medicine), Yale University School of Medicine, PO Box 208064, 333 Cedar Street, New Haven, CT 06520-8064, USA. enboma@biomed.med.yale.edu
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D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D015152:Blotting, Northern; D002490:Central Nervous System / Q000378:metabolism; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014157:Transcription Factors
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019476:Insect Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors