title: Shini-san inhibits mast cell-dependent immediate-type allergic reactions.
abstract: Shini-San has been used for treatment of allergic disease in Korea. However, its effect in experimental models remains unknown. The mast cell plays a pivotal role in initiating allergic response by secreting intracytoplasmic granular mediators such as histamine. The present report describes an inhibitory effect of Shini-San on mast cell-mediated immediate-type allergic reactions. Topical application of compound 48/80 can induce an ear swelling response in normal (WBB6F1(-)+/+) mice but not in congenic mast cell-deficient WBB6F1-W/WV mice. Shini-San inhibited concentration-dependent mast cell-dependent ear swelling response induced by compound 48/80 in normal mice. Shini-San inhibited concentration-dependent passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP) immunoglobulin E (IgE) in rats by topical application. Shini-San also inhibited in concentration-dependent fashion the histamine release from the rat peritoneal mast cells by compound 48/80 or anti-DNP IgE. Moreover, Shini-San had a significant inhibitory effect on compound 48/80-induced systemic anaphylactic reaction. These results indicate that Shini-San inhibits immediate type allergic reactions by inhibition of mast cell degranulation in vivo and in vitro.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea.
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: H J
authors.initials: HJ
authors.lastname: Chae
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Baek
authors.forename: K S
authors.initials: KS
authors.lastname: Lim
authors.forename: C Y
authors.initials: CY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D004364:Pharmaceutical Preparations; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: D018926:Anti-Allergic Agents; D004364:Pharmaceutical Preparations; D010936:Plant Extracts; C403257:shini-san; D003189:p-Methoxy-N-methylphenethylamine