title: Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.
abstract: We report on a series of Spanish patients with acute lymphoblastic leukaemia in whom the t(12;21) [TEL/AML1] translocation could not be identified with two sensitive techniques: reverse transcript-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH). 101 cases were analysed: 38 children (29 B-cell precursor; nine T-cell precursor) and 63 adults (48 B-cell precursor; 15 T-cell precursor). Specific RT-PCR to amplify the TEL/AML1 fusion transcript was negative in all 101 cases. Moreover, all 38 paediatric samples were also negative by interphase FISH analysis for the presence of the TEL/AML1 fusion. These results suggest the existence of geographic/race variations in the genotype of acute lymphoblastic leukaemia (ALL).
authors.affiliation: Haematology Service, University Hospital of Salamanca, Instituto de Biología Molecular y Celular del Cáncer, Universidad de Salamanca, Salamanca, Spain.
authors.forename: R
authors.initials: R
authors.lastname: García-Sanz
authors.forename: I
authors.initials: I
authors.lastname: Alaejos
authors.forename: A
authors.initials: A
authors.lastname: Orfão
authors.forename: A
authors.initials: A
authors.lastname: Rasillo
authors.forename: M C
authors.initials: MC
authors.lastname: Chillón
authors.forename: M D
authors.initials: MD
authors.lastname: Tabernero
authors.forename: M V
authors.initials: MV
authors.lastname: Mateos
authors.forename: R
authors.initials: R
authors.lastname: López-Pérez
authors.forename: D
authors.initials: D
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: Balanzategui
authors.forename: M
authors.initials: M
authors.lastname: González
authors.forename: J F
authors.initials: JF
authors.lastname: San Miguel
authors.forename: A
authors.initials: A
authors.lastname: Bortolucci
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit; D005260:Female; D005787:Gene Frequency; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000453:epidemiology / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013030:Spain / Q000453:epidemiology; D014178:Translocation, Genetic / Q000235:genetics*; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein