Conjugal transfer of a TOL-like plasmid and extension of the catabolic potential of Pseudomonas putida F1.
Strain mX was isolated from a petrol-contaminated soil, after enrichment on minimal medium with 0.5% (v/v) meta-xylene as a sole carbon source. The strain was tentatively characterized as Pseudomonas putida and harboured a large plasmid (pMX) containing xyl genes involved in toluene or meta-xylene degradation pathways via an alkyl monooxygenase and a catechol 2,3-dioxygenase. This new TOL-like plasmid was stable over two hundred generations and was self-transferable. After conjugal transfer to P. putida F1, which possesses the Tod chromosomal toluene biodegradative pathway, the transconjugant P. putida F1(pMX) was able to grow on benzene, toluene, meta-xylene, para-xylene, and ethylbenzene compounds as the sole carbon sources. Catechol 2,3-dioxygenases of the transconjugant cells presented a more relaxed substrate specificity than those of parental cells (strain mX and P. putida F1).
Hallier-Soulier
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S
CIRSEE (Centre International de Recherches Sur l'Eau et l'Environment) 38, Le Pecq, France.
Ducrocq
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Truffaut
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N
D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D002396:Catechols / Q000378:metabolism; D003227:Conjugation, Genetic; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D049308:Dioxygenases; D004586:Electrophoresis / Q000379:methods; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism; D010578:Petroleum; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants; D013379:Substrate Specificity; D014050:Toluene / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism
D002396:Catechols; D003470:Culture Media; D004269:DNA, Bacterial; D006841:Hydrocarbons, Aromatic; D010578:Petroleum; D012989:Soil Pollutants; D014992:Xylenes; D014050:Toluene; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase