title: Characterization of paxillin LIM domain-associated serine threonine kinases: activation by angiotensin II in vascular smooth muscle cells.
abstract: Recently we reported a novel means of regulating LIM domain protein function. Paxillin LIM zinc-finger phosphorylation in response to cell adhesion regulates the subcellular localization of this cytoskeletal adaptor protein to focal adhesions, and also modulates cell adhesion to fibronectin (Brown et al. [1998] Mol. Biol. Cell 9:1803-1816). In the present study, we characterize further the protein kinases that phosphorylate paxillin LIM2 on threonine and LIM3 on serine. Analysis of the subcellular distribution of the LIM kinases demonstrated that the LIM3 protein kinase, but not the LIM2 kinase, resides within a detergent-insoluble fraction. The activities of the paxillin LIM domain kinases are differentially regulated during embryogenesis, and analysis of tissue distribution indicated a specificity in expression patterns between the LIM2 and LIM3 kinases. In addition, these protein kinases were refractory to inhibition by a panel of broad-spectrum serine/threonine kinase inhibitors, suggesting a novel derivation. The paxillin protein kinase activities were stimulated in serum-starved CHO.K1 cells by the mitogen phorbol myristate acetate (PMA), and by PMA and angiotensin II in rat aortic smooth muscle cells. In vivo labeling, phosphoamino acid analysis, and phosphopeptide mapping of paxillin immunoprecipitated from angiotensin II-stimulated smooth muscle cells confirmed an induction of paxillin serine/threonine phosphorylation and supports the contention that these newly identified paxillin kinases are dynamic components of growth factor signaling through the cytoskeleton.
authors.affiliation: Department of Anatomy and Cell Biology, Program in Cell and Molecular Biology, State University of New York Health Science Center at Syracuse, Syracuse, New York 13210, USA. brownm@vax.cs.hscsyr.edu
authors.forename: M C
authors.initials: MC
authors.lastname: Brown
authors.forename: C E
authors.initials: CE
authors.lastname: Turner
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D002642:Chick Embryo; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D006133:Growth Substances / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051419:Paxillin; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction
chemical_list: D003598:Cytoskeletal Proteins; D006133:Growth Substances; D016023:Integrins; D051419:Paxillin; D010750:Phosphoproteins; C496063:Pxn protein, rat; D000804:Angiotensin II; D017346:Protein Serine-Threonine Kinases