title: Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus.
abstract: The nor-1 gene is involved in aflatoxin biosynthesis in Aspergillus parasiticus and was predicted to encode a norsolorinic acid ketoreductase. Recombinant Nor-1 expressed in Escherichia coli converted the 1' keto group of norsolorinic acid to the 1' hydroxyl group of averantin in crude E. coli cell extracts in the presence of NADPH. The results confirm that Nor-1 functions as a ketoreductase in vitro.
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824-1224, USA.
authors.forename: R
authors.initials: R
authors.lastname: Zhou
authors.forename: J E
authors.initials: JE
authors.lastname: Linz
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D000426:Alcohol Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology*; D003001:Cloning, Molecular; D004926:Escherichia coli; D005656:Fungal Proteins; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000348:Aflatoxins; D005656:Fungal Proteins; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C062609:norsolorinic acid ketoreductase