Examination of ras oncoproteins in human plasma from healthy controls and workers exposed to petroleum emissions, including benzene-related compounds.
Ras oncoproteins in blood plasma from workers exposed to petroleum emissions and unexposed controls were examined from Polish and Estonian samples. Twenty-four workers and 35 unexposed controls were examined from Poland and 97 exposed and 40 unexposed controls from Estonia. Of the Estonian workers, 50 were exposed to benzene in a benzene production plant and 47 to polyaromatic hydrocarbons and benzene in a cokery. Blood plasma proteins were separated by gel electrophoresis, transferred to a nitrocellulose membrane by Western blotting and detected by chemiluminescence using a monoclonal antibody as the primary antibody. There were no statistically significant differences between the exposed and the control groups in either the Polish or the Estonian samples.
Anderson
D
D
BIBRA International, Carshalton, Surrey, UK. danderson@bibra.co.uk
Hughes
J A
JA
Brinkworth
M H
MH
Cebulska-Wasilewska
A
A
Nizankowska
E
E
Graca
B
B
Veidebaum
T
T
Peltonen
K
K
Sorsa
M
M
D000395:Air Pollutants, Occupational / Q000097:blood*; D001554:Benzene / Q000032:analysis*; D001555:Benzene Derivatives / Q000032:analysis*; D015415:Biomarkers / Q000097:blood*; D015153:Blotting, Western; D002617:Chemical Industry; D004591:Electrophoresis, Polyacrylamide Gel; D004784:Environmental Monitoring / Q000379:methods; D004957:Estonia; D006801:Humans; D016273:Occupational Exposure / Q000032:analysis*; D010578:Petroleum; D011044:Poland; D016283:Proto-Oncogene Proteins p21(ras) / Q000097:blood*; D012621:Seasons
D000395:Air Pollutants, Occupational; D001555:Benzene Derivatives; D015415:Biomarkers; D010578:Petroleum; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D001554:Benzene