title: Free thiol groups are essential for infectivity of human cytomegalovirus.
abstract: The membrane-impermeable thiol blocker 5'5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.
authors.affiliation: Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
authors.forename: Ali
authors.initials: A
authors.lastname: Mirazimi
authors.affiliation: Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Mousavi-Jazi
authors.affiliation: Department of Immunology, Microbiology, Pathology and Infectious Disease, Karolinska Institute, Box 12 773, 112 96 Stockholm, Sweden 2.
authors.forename: Vivi-Anne
authors.initials: VA
authors.lastname: Sundqvist
authors.affiliation: Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
authors.forename: Lennart
authors.initials: L
authors.lastname: Svensson
mesh_terms: D003587:Cytomegalovirus / Q000187:drug effects / Q000472:pathogenicity*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006801:Humans; D013438:Sulfhydryl Compounds / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000502:physiology
chemical_list: D013438:Sulfhydryl Compounds; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D004228:Dithionitrobenzoic Acid; D004229:Dithiothreitol