Immobilization of cholesterol oxidase on Formvar using organic solvents.
The present study describes a new immobilization support for the preparation of enzyme membrane in the presence of organic solvents. The support was composed of Formvar solubilization in organic solvents with enzyme. More than 90% of the enzyme was immobilized on the membrane. The membrane was prepared by mixing 4% Formvar in organic solvents and 1% cholesterol oxidase was immobilized by entrapment technique. Practically no leaching of the entrapped enzyme was observed. The resultant immobilized enzyme membrane was stored at 4 degrees C for 10 days without losing its activity. The pH and temperature stabilities were greater than those of the native enzyme. The immobilized enzyme membrane has a long life due to its hydrophobic nature, as compared with other membranes.
Kumar
H
H
Centre for Biochemical Technology, Mall Road, Delhi-110007, India.
Kumar
A
A
Kumari
P
P
Jyotirmai
S
S
Tulsani
N B
NB
D002789:Cholesterol Oxidase / Q000737:chemistry* / Q000378:metabolism; D003124:Colorimetry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013696:Temperature
D004800:Enzymes, Immobilized; D009930:Organic Chemicals; D011145:Polyvinyls; D012997:Solvents; C013215:polyvinyl acetate; D002789:Cholesterol Oxidase