Sheathless preconcentration-capillary zone electrophoresis-mass spectrometry applied to peptide analysis.
A reliable capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry system has been developed for the sensitive analysis and sequencing of peptides. The system comprises (1) a zero dead volume on-line sample preconcentration interface allowing over 100-fold increase in sample volume introduction and (2) a zero-dead volume, durable electrospray emitter yielding stable, low background electrospray signals, both proving not to compromize the electrophoretic performance. Sub-fmol sensitivities were obtained in applications to complex peptide samples derived from tryptic digestion of proteins and naturally processed major histocompatibility complex class I associated peptides.
Barroso
M B
MB
National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
de Jong
A P
AP
D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D005805:Genes, MHC Class I; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D007202:Indicators and Reagents; D010455:Peptides; D011506:Proteins