[The establishment and application of genomic DNA extraction from peripheral blood].
OBJECTIVE
To establish the method of genomic DNA extraction.


METHODS
Genomic DNA was directly extracted from peripheral blood with the use of potassium iodide.


RESULTS
The genomic DNA extracted with potassium iodide was of large molecular weight. A(260)/A(280) and A(260)/ A(230) were 1.85 and 2.2 respectively. The extraction efficiency was greater than 90%. The result of PCR for the DNA was good.


CONCLUSIONS
The method is simple, quick, economical and could be used for studying numbers of clinical samples.
Zhao
S
S
No.88 Hospital of PLA, Tai'an 271000 P.R.China. 88hosp@public.taptt.sd.cn
Zhang
J
J
You
J
J
Yang
Y
Y
Guo
F
F
D004247:DNA / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D011193:Potassium Iodide
D011193:Potassium Iodide; D004247:DNA