title: Effect of hypertonic saline and alcohol on viability of daughter cysts in hepatic hydatid disease.
abstract: OBJECTIVE
  To test the efficacy of hypertonic saline (20%) and absolute alcohol on the integrity of daughter cysts and the viability of the protoscoleces contained in these cysts.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Teaching hospital, Turkey.
  
  
  METHODS
  80 daughter cysts obtained from two patients with Gharbi type III hydatid cysts of the liver.
  
  
  METHODS
  The cysts were divided into two groups, in the first of which cysts were placed into hypertonic saline and in the second into absolute alcohol; they were kept there for 5, 15, 30 or 60 minutes.
  
  
  METHODS
  Integrity of the cyst wall and viability of the contents were evaluated using a vital staining technique with 0.1% eosin.
  
  
  RESULTS
  Neither hypertonic saline nor absolute alcohol solution had any effect on the integrity of the daughter cysts or the viability of the protoscoleces.
  
  
  CONCLUSIONS
  Percutaneous drainage of type III hydatid cysts can lead to high recurrence rates.
authors.affiliation: Department of General Surgery, Ankara University Faculty of Medicine, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Karayalçin
authors.forename: H
authors.initials: H
authors.lastname: Besim
authors.forename: M
authors.initials: M
authors.lastname: Sonisik
authors.forename: N
authors.initials: N
authors.lastname: Erverdi
authors.forename: A
authors.initials: A
authors.lastname: Korkmaz
authors.forename: N
authors.initials: N
authors.lastname: Aras
mesh_terms: D002470:Cell Survival; D002473:Cell Wall / Q000502:physiology; D004322:Drainage; D004444:Echinococcosis, Hepatic / Q000628:therapy*; D000431:Ethanol / Q000627:therapeutic use*; D006801:Humans; D012462:Saline Solution, Hypertonic / Q000627:therapeutic use*
chemical_list: D012462:Saline Solution, Hypertonic; D000431:Ethanol