Identification of salt-regulated genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803 by subtractive RNA hybridization.
To identify genes transcribed preferentially under salt stress, a subtractive RNA hybridization procedure was applied to the cyanobacterium Synechocystis sp. PCC 6803. The screening of a genomic library led to the identification of several RNA species that were more abundant in salt-stressed cells than in control cells. Salt-dependent transcription of the identified genes was verified in Northern blot experiments. In addition to the previously characterized genes cpn60 (encoding GroEL; a molecular chaperone) and isiA (encoding a chlorophyll-binding protein), genes encoding a protein of unknown function (slr0082) and a putative RNA helicase (slr0083) were identified as salt-regulated genes in Synechocystis. Genes slr0082 and slr0083, located at sites adjacent to each other on the Synechocystis chromosome, were transcribed from separate promoters and showed the most significant induction 1-3 h after salt shock. The salt-regulated promoters of these two genes were mapped. Genes cpn60, slr0082, and slr0083 were also found to be induced by a cold shock. The possible role of the identified gene products for salt adaptation of Synechocystis is discussed.
Vinnemeier
J
J
Universität Rostock, Fachbereich Biologie, Doberaner Strasse 143, D-18051 Rostock, Germany.
Hagemann
M
M
D001483:Base Sequence; D002874:Chromosome Mapping; D003080:Cold Temperature; D000458:Cyanobacteria / Q000187:drug effects / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology
D017931:DNA Primers; D012329:RNA, Bacterial; D012965:Sodium Chloride