Effect of active molluscicidal component of spices on different enzyme activities and biogenic amine levels in the nervous tissue of Lymnaea acuminata.
In vivo exposure of Lymnaea acuminata to thymol and [6]-gingerol (active molluscicidal components of Trachyspermum ammi and Zingiber officinale, respectively) indicates that they significantly alter acetylcholinesterase, lactic dehydrogenase, succinic dehydrogenase and cyto-oxidase activity in the nervous -tissue of snails. In vitro exposure showed that, except for acetylcholinesterase and lactic dehydrogenase, no significant changes were observed in cyto-oxidase and succinic dehydrogenase activity in the nervous tissue of L. acuminata. Sublethal exposure to thymol and [6]-gingerol reduced the levels of 5-hydroxytryptamine (5-HT) and dopamine (DA) in the nervous tissue of L. acuminata. There was, however, no significant change in the level of 5-hydroxy indol acetic acid (5-HIAA). Thymol and [6]-gingerol thus affects all the known neurotransmission mechanisms in the snail either separately or through a complex interaction between the different neurotransmitters. This may account for their toxicity to snails.
Singh
V K
VK
Department of Zoology, DDU Gorakhpur University, Gorakhpur PIN-273 009, U.P., India.
Singh
S
S
Singh
D K
DK
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D002396:Catechols; D004298:Dopamine / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005233:Fatty Alcohols / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008975:Molluscacides / Q000494:pharmacology*; D009417:Nerve Tissue / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D012908:Snails / Q000201:enzymology*; D017365:Spices; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013943:Thymol / Q000494:pharmacology*
D001679:Biogenic Amines; D002396:Catechols; D005233:Fatty Alcohols; D008975:Molluscacides; D010936:Plant Extracts; D012701:Serotonin; D013943:Thymol; D006897:Hydroxyindoleacetic Acid; C007845:gingerol; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000110:Acetylcholinesterase; D004298:Dopamine