title: IgE antibody response to vertebrate meat proteins including tropomyosin.
abstract: BACKGROUND
  Although meat is a main source of proteins in western diets, little information is available regarding allergy to vertebrate meats or the allergens implicated in these reactions.
  
  
  OBJECTIVE
  To evaluate the in vitro IgE antibody response to different vertebrate meats in suspected meat-allergic subjects, as well as the possible role of tropomyosin in meat allergy and to analyze the cross-reactivity between vertebrate meats and the effect of heating on the IgE-binding to meat proteins.
  
  
  METHODS
  Fifty-seven sera from suspected meat-allergic subjects were tested by grid blot to extracts of beef, lamb, pork, venison, chicken, and turkey and to four mammalian tropomyosins of different origins.
  
  
  RESULTS
  Meat-allergic subjects have IgE antibodies to proteins in different mammalian meats (43/57 subjects); cross-reactivity with avian meat was limited: less than 50% (19/43) of meat positive sera reacted to chicken. In contrast, most of the poultry-positive sera also reacted to different mammalian meats. In general, there was stronger IgE reactivity to raw meats in comparison to cooked meats; an exception was six cases in which IgE reactivity to cooked poultry was stronger. Weak IgE reactivity to tropomyosin was detected in only 2/57 sera tested.
  
  
  CONCLUSIONS
  Suspected meat-allergic subjects have serum IgE directed to meat proteins. In vitro cross-reactivity among mammalian meats appears to be important, while cross-reactivity to poultry is limited indicating mammalian-specific proteins. Although cooking in general denatures meat proteins rendering them less allergenic, in some cases the process of cooking may result in the formation of new allergenic moieties. The muscle protein tropomyosin is not an important vertebrate meat allergen.
authors.affiliation: Section of Allergy & Clinical Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
authors.forename: R
authors.initials: R
authors.lastname: Ayuso
authors.forename: S B
authors.initials: SB
authors.lastname: Lehrer
authors.forename: L
authors.initials: L
authors.lastname: Tanaka
authors.forename: M D
authors.initials: MD
authors.lastname: Ibañez
authors.forename: C
authors.initials: C
authors.lastname: Pascual
authors.forename: A W
authors.initials: AW
authors.lastname: Burks
authors.forename: G L
authors.initials: GL
authors.lastname: Sussman
authors.forename: B
authors.initials: B
authors.lastname: Goldberg
authors.forename: M
authors.initials: M
authors.lastname: Lopez
authors.forename: G
authors.initials: G
authors.lastname: Reese
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002417:Cattle; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D003296:Cooking; D004591:Electrophoresis, Polyacrylamide Gel; D005512:Food Hypersensitivity / Q000209:etiology* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007223:Infant; D008297:Male; D008460:Meat / Q000009:adverse effects*; D008875:Middle Aged; D011506:Proteins / Q000276:immunology; D014335:Tropomyosin / Q000276:immunology*
chemical_list: D011506:Proteins; D014335:Tropomyosin; D007073:Immunoglobulin E