Role of adult sheep in transmission of infection by Cryptosporidium parvum to lambs: confirmation of periparturient rise.
In sheep farms, oocyst shedding by asymptomatic adult carriers is one of the mechanisms which may explain maintenance of infections by Cryptosporidium parvum between lambing periods. The objective of this work was to investigate this hypothesis and the existence of a periparturient rise in oocyst shedding. Fourteen pregnant sheep were randomly selected from two farms with a history of neonatal diarrhoea caused by C. parvum and samples were collected from the 6th week before birth until 2 weeks after birth. Faecal samples were filtered, concentrated and examined for oocysts using an indirect immunofluorescence assay. The kinetics of anti-C. parvum antibodies (IgG and IgA) were studied using an indirect enzyme-linked immunosorbent assay. All except one animal excreted C. parrum oocysts at some time during the experimental period. The percentage of animals passing oocysts increased in the first week post-partum (farm 1) and in the first week before birth (farm 2). The numbers of oocysts excreted ranged from 20-440 oocysts g(-1) of faeces. In contrast, no significant changes in the anti-C. parvum immunoglobulin levels were observed over the sampling period. Finally, a high percentage of lambs (71%) born to these ewes acquired infection in the first 2 weeks of life.
Ortega-Mora
L M
LM
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain. luisucm@eucmax.sim.ucm.es
Requejo-Fernández
J A
JA
Pilar-Izquierdo
M
M
Pereira-Bueno
J
J
D000818:Animals; D000831:Animals, Newborn; D000913:Antibodies, Protozoan / Q000097:blood; D003457:Cryptosporidiosis / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007743:Labor, Obstetric; D010270:Parasite Egg Count; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology / Q000635:transmission*
D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G