Theoretical study of mexiletine and its interaction with cationic and anionic receptor sites.
Theoretical methods are applied to study the antiarrhythmic (AA) mexiletine (1-(2,6-dimethylphenoxy)-2-aminopropane). The AM1 method is used to construct a three-centre binding model for this drug. This model consists of an amine nitrogen atom that is protonated to a higher degree at physiological pH, flat hydrophobic regions of aromatic rings and additional functional groups with lone electron pairs of oxygen. Based on these ideas, a model for the binding of mexiletine at the transmembrane protein was constructed. An ab initio SCF method was used to study the two-component mexiletine-receptor binding site composed of acetate (Glu-, Asp-) and protonated methylamine (Lys+, Arg+). The binding of mexiletine to the receptor may be understood by considering a two-step process of recognition and binding of AA to its receptor. Within this model the mexiletine cation is recognised in the first step and bonded to the negatively-charged part of the receptor. In a subsequent step, the interaction between the amide oxygen and cationic amine group of the membrane protein may follow.
Remko
M
M
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia. remko@pharm.uniba.sk
Smiesko
M
M
Benová
A
A
D000889:Anti-Arrhythmia Agents / Q000737:chemistry*; D001665:Binding Sites; D008801:Mexiletine / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011955:Receptors, Drug / Q000737:chemistry* / Q000378:metabolism; D013237:Stereoisomerism
D000889:Anti-Arrhythmia Agents; D011955:Receptors, Drug; D008801:Mexiletine