title: Pre-column derivatization and gas chromatographic determination of alkaloids in bulbs of Fritillaria.
abstract: A method of precolumn derivatization GC with FID detection was developed for a simultaneous analysis of five major steroidal alkaloids of Fritillaria species, namely ebeiedine, ebeiedinone, verticine, verticinone and imperialine. Derivatization was carried out by trimethylsilylation of the hydroxyl-containing Fritillaria alkaloids to the corresponding trimethylsilylates with trimethylsilylimidazole. Reaction conditions were optimised and the alkaloids derivatives were characterised by on-line GC-MS. The validated GC method demonstrated a good linearity at the sampling ranges used. This analytical method is simple, convenient and reproducible. The developed assay was successfully applied to the determination of the major pharmacologically active alkaloids in three commonly used antitussive Fritillaria species: F. cirrhosa, F. thunbergii and F. pallidiflora.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing.
authors.forename: S L
authors.initials: SL
authors.lastname: Li
authors.forename: S W
authors.initials: SW
authors.lastname: Chan
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: G
authors.initials: G
authors.lastname: Lin
authors.forename: G H
authors.initials: GH
authors.lastname: Zhou
authors.forename: Y J
authors.initials: YJ
authors.lastname: Ren
authors.forename: F C
authors.initials: FC
authors.lastname: Chiu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D000996:Antitussive Agents / Q000032:analysis*; D002595:Cevanes / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry
chemical_list: D000470:Alkaloids; D000996:Antitussive Agents; D002595:Cevanes; D004365:Drugs, Chinese Herbal; C014242:verticine; C047331:peiminine