title: Structural definition of arabinomannans from Mycobacterium bovis BCG.
abstract: The structures of the hydrophilic parietal and cellular arabinomannans isolated from Mycobacterium bovis BCG cell wall [Nigou et al. (1997) J Biol Chem 272: 23094-103] were investigated. Their molecular mass as determined by MALDI-TOF mass spectrometry was around 16 kDa. Concerning cap structure, capillary electrophoresis analysis demonstrated that dimannoside (Manpalpha1-->2Manp) was the most abundant motif (65-75%). Using two-dimensional 1H-13C NMR spectroscopy, the mannan core was unambiguously demonstrated to be composed of -->6Manpalpha1--> backbone substituted at some O-2 by a single Manp unit. The branching degree was determined as 84%. Finally, arabinomannans were found to be devoid of the phosphatidyl-myo-inositol anchor and, by aminonaphthalene disulfonate tagging, the mannan core was shown to contain a reducing end. This constitutes the main difference between arabinomannans and lipoarabinomannans from Mycobacterium bovis BCG.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du Centre National de la Recherche Scientifique, UPR 9062, Toulouse, France.
authors.forename: J
authors.initials: J
authors.lastname: Nigou
authors.forename: M
authors.initials: M
authors.lastname: Gilleron
authors.forename: T
authors.initials: T
authors.lastname: Brando
authors.forename: A
authors.initials: A
authors.lastname: Vercellone
authors.forename: G
authors.initials: G
authors.lastname: Puzo
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008351:Mannans; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C012189:arabinomannan; D008358:Mannose