Influence of HgCl2 on the osphradial multisensory system of Lymnaea stagnalis L.
The osphradial multisensory system of Lymnaea stagnalis L. (Pulmonata, Basommatophora) was used to demonstrate the modulation of chemosensory information both at periphery and central nervous system (CNS) following heavy metal treatments. A semi-intact preparation including osphradium, CNS and the right inner parietal nerve (r.i.p.n.) connecting them was used to record both extracellular activity of nerve and intracellular activity of central neurons receiving information from osphradium. The ion currents of osphradium were recorded using patch-clamp method. The changes in nerve and neuronal activity were expressed by averaging of firing frequency and interspike intervals. The chemosensory function of osphradium was shown by application of L-aspartate, urea, saccharose and stagnant water to its surface. The central neurons reacting to the stimulation ofosphradium were located to visceral, right parietal, pedal and cerebral ganglia of Lymnaea. Both the acute and chronic treatments with HgCl2 damaged the sensory function of osphradium traced on the flow of information from periphery to central neurons. At the same time, mercury chloride modified the synaptic connections of respiratory pattern generators as well as the Ca- and K-dependent ion currents of osphradial neurons. The results proved the multisensory role of osphradium sensing the alterations in the environment and its usefulness in monitoring the effects of pollutants at various level of regulation from chemosensory epithelium to CNS.
Kamardin
N N
NN
Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany.
Szücs
A
A
S-Rózsa
K
K
D000818:Animals; D002628:Chemoreceptor Cells / Q000187:drug effects*; D006321:Heart / Q000187:drug effects / Q000502:physiology; D008195:Lymnaea / Q000502:physiology*; D008627:Mercuric Chloride / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D012119:Respiration / Q000187:drug effects
D008627:Mercuric Chloride