A deletion on chromosome 4 cosegregates with the whirler deafness mutation: exclusion of Orm1 as a candidate.
Whirler (wi) mice display profound deafness and a head-tossing and circling phenotype, showing an autosomal recessive mode of inheritance. The wi mutation has been shown to map close to the Orm gene cluster on mouse Chromosome (Chr) 4. We have, therefore, investigated the Orm loci as candidates for the whirler gene. Detailed mapping and analysis of the Orm gene cluster in both normal and whirler mice indicates the presence of a <48-kb deletion in whirler mice that disrupts the Orm1 locus. The Orm1 locus is also deleted in the CE/J mouse strain, and we discuss the candidature of Orm1 for the whirler gene.
Paige
A J
AJ
MRC Mammalian Genetics Unit and Mouse Genome Centre, Harwell, Didcot, Oxon OX11 0RD, UK.
Kiernan
B W
BW
Varela
A
A
Rogers
M J
MJ
Hughes
D
D
Steel
K P
KP
Brown
S D
SD
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D020090:Chromosome Segregation / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D003638:Deafness / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D017353:Gene Deletion; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains; D005810:Multigene Family; D009961:Orosomucoid / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D015342:DNA Probes; D009961:Orosomucoid; D004247:DNA