Interleukin-2 receptoralpha gene expression in intestinal allograft rejection.
This study was undertaken to determine whether the interleukin-2 receptor alpha (IL-2Ralpha) coding gene could serve as a genetic marker of intestinal allograft rejection. The small bowel graft from Jinbai mice was heterotopically transplanted to BALB/c mice. Naive BALB/c mice and BALB/c mice with intestinal isografts were used as controls. The mRNA transcription level of the spleen cells harvested from the recipients and controls was measured with quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) on postoperative day (POD) 3 and routine pathology was studied in these grafts. The IL-2Ralpha mRNA transcription level in untreated allografts was significantly higher than that of controls on POD 3, when intestinal allograft rejection was not detected. These data suggest that the IL-2Ralpha mRNA transcription level may reflect the early cellular immune response and this genetic marker may be of value in monitoring intestinal allograft rejection.
Zhu
L
L
Department of Surgery, Tianjin Medical University, Tianjin Medical University Hospital and Tianjin Research Institute of Experimental Surgery, Tianjin, P. R. China.
Wang
P
P
Luo
Y
Y
Lu
N
N
Wang
H
H
D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression; D005819:Genetic Markers; D006084:Graft Rejection / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D014158:Transcription, Genetic; D014184:Transplantation, Homologous
D005819:Genetic Markers; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2