title: A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy.
abstract: Methylotrophic yeasts contain large peroxisomes during growth on methanol. Upon exposure to excess glucose or ethanol these organelles are selectively degraded by autophagy. Here we describe the cloning of a Pichia pastoris gene (PpVPS15) involved in peroxisome degradation, which is homologous to Saccharomyces cerevisiae VPS15. In methanol-grown cells of a P. pastoris VPS15 deletion strain, the levels of peroxisomal marker enzymes remained high after addition of excess glucose or ethanol. Electron microscopic studies revealed that the organelles were not taken up by vacuoles, suggesting that PpVPS15 is required at an early stage in peroxisome degradation.
authors.affiliation: Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Biological Centre, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: O V
authors.initials: OV
authors.lastname: Stasyk
authors.forename: I J
authors.initials: IJ
authors.lastname: van der Klei
authors.forename: A R
authors.initials: AR
authors.lastname: Bellu
authors.forename: S
authors.initials: S
authors.lastname: Shen
authors.forename: J A
authors.initials: JA
authors.lastname: Kiel
authors.forename: J M
authors.initials: JM
authors.lastname: Cregg
authors.forename: M
authors.initials: M
authors.lastname: Veenhuis
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport; D000431:Ethanol / Q000494:pharmacology; D017353:Gene Deletion; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000187:drug effects / Q000648:ultrastructure*; D010843:Pichia / Q000187:drug effects / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D058571:Vacuolar Sorting Protein VPS15
chemical_list: D017931:DNA Primers; D056827:Endosomal Sorting Complexes Required for Transport; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D017346:Protein Serine-Threonine Kinases; C546854:VPS15 protein, S cerevisiae; D058571:Vacuolar Sorting Protein VPS15; D005947:Glucose