Possible susceptibility of the HLA-DPB1*0402 and HLA-DPB1*04 alleles to unexplained recurrent abortion: analysis by means of polymerase chain reaction-restricted fragment length polymorphism method.
OBJECTIVE
To clarify whether HLA-DP antigens are associated with patient population of unexplained recurrent abortion.


METHODS
The frequency of HLA-DPB1 alleles in patients with unexplained recurrent abortion, and the compatibility of HLA-DPB1 alleles between patient couples, were studied using a polymerase chain reaction (PCR)-restricted fragment length polymorphism (RFLP) method. Thirty patients who had a history of unexplained primary recurrent abortion, and their husbands, were typed for HLA-DPB1 genotype. Two hundred and ninety-nine base pair fragments from the second exon of HLA-DPB1 genes were selectively amplified using the PCR-primers. After amplification, the DNAs were digested with restriction endonucleases, and subjected to electrophoresis in a 12% polyacrilamide gel to determine HLA-DPB1 genotype.


RESULTS
The frequency of HLA-DPB1*0402 and DPB1*04 alleles in the patient group (n = 30) was significantly increased, as compared to that in the normal fertile women (n = 30). The frequency of HLA-DPB1*04 allele in the patient group was significantly increased, as compared to that in the general population (n = 112). No significant compatibility of HLA-DPB1 alleles could be observed between patient couples and normal fertile couples.


CONCLUSIONS
These findings suggest a possible new class II association with patient population of unexplained recurrent abortion.
Takakuwa
K
K
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
Hataya
I
I
Arakawa
M
M
Kikuchi
A
A
Higashino
M
M
Yasuda
M
M
Kurabayashi
T
T
Tanaka
K
K
D000026:Abortion, Habitual / Q000235:genetics* / Q000276:immunology; D000483:Alleles; D005220:Fathers; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006682:HLA-DP Antigens / Q000235:genetics* / Q000276:immunology; D059868:HLA-DP beta-Chains; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen