title: Tn5: A molecular window on transposition.
abstract: DNA transposition is an underlying process involved in the remodeling of genomes in all types of organisms. We analyze the multiple steps in cut-and-paste transposition using the bacterial transposon Tn5 as a model. This system is particularly illuminating because of the existence of structural, genetic, and biochemical information regarding the two participating specific macromolecules: the transposase and the 19-bp sequences that define the ends of the transposon. However, most of the insights should be of general interest because of similarities to other transposition-like systems such as HIV-1 DNA integration into the host genome.
authors.affiliation: Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin, 53706, USA. reznikoff@biochem.wisc.edu
authors.forename: W S
authors.initials: WS
authors.lastname: Reznikoff
authors.forename: A
authors.initials: A
authors.lastname: Bhasin
authors.forename: D R
authors.initials: DR
authors.lastname: Davies
authors.forename: I Y
authors.initials: IY
authors.lastname: Goryshin
authors.forename: L A
authors.initials: LA
authors.lastname: Mahnke
authors.forename: T
authors.initials: T
authors.lastname: Naumann
authors.forename: I
authors.initials: I
authors.lastname: Rayment
authors.forename: M
authors.initials: M
authors.lastname: Steiniger-White
authors.forename: S S
authors.initials: SS
authors.lastname: Twining
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D011485:Protein Binding; D019895:Transposases / Q000737:chemistry / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D004247:DNA; D019895:Transposases