title: Analyses of lipopolysaccharides, outer membrane proteins and DNA fingerprints reveal intraspecies diversity in Moraxella bovis isolated in Argentina.
abstract: Intra-specific diversity within Moraxella bovis was investigated analysing DNA fingerprints, outer membrane proteins (OMP) and lipopolysaccharides (LPS) profiles. Three collection strains and 57 isolates of M. bovis, collected during 3 years from cattle with infectious bovine keratoconjunctivitis (IBK) symptoms, from diverse geographical locations of Argentina, were examined. The LPS and OMP profiles were studied through SDS-PAGE analysis and genotype was determined by PCR-DNA fingerprinting. Genotyping identified five DNA types while analysis of LPS and OMP profiles identified three rough LPS types and three OMP types among the 60 isolates of M. bovis including the three collection strains. None of the three methods employed to assess diversity was discriminating when used alone because the degree of heterogeneity in each group of surface structures was limited, but when data of each typing method were combined, 15 distinct subgroups were determined. This subgrouping was clearly able to differentiate isolates of the same genotype. These typing methods appear to be useful to assess different aspects of the disease such as the diversity within a population of M. bovis associated to epidemic conditions, track the causal agent in an outbreak of the disease, monitoring vaccination programs and studies on virulence.
authors.affiliation: Centro de Investigación y Desarrollo en Fermentaciones Industriales, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
authors.forename: C I
authors.initials: CI
authors.lastname: Prieto
authors.forename: O M
authors.initials: OM
authors.lastname: Aguilar
authors.forename: O M
authors.initials: OM
authors.lastname: Yantorno
mesh_terms: D000818:Animals; D001118:Argentina; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005838:Genotype; D008070:Lipopolysaccharides / Q000737:chemistry*; D016955:Moraxella bovis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016870:Neisseriaceae Infections / Q000382:microbiology* / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides