Heterogeneity of anti-dsDNA antibodies in their cross-reaction with ribosomal P protein.
We have investigated the possible cross-reaction of anti-dsDNA antibodies with ribosomal P peptide for several reasons. First, the antibodies frequently occur together, and secondly, they vary similarly with disease activity. Human polyclonal anti-dsDNA antibodies were affinity purified from eight patients and anti-ribosomal P antibodies from two patients with systemic Lupus erythematosus (SLE) who had high titers of anti-dsDNA as well as anti-ribosomal P antibodies. Nine of the 10 sera were totally specific in their reactivity with their cognate antigens. In only one patient did we find a subpopulation of antibodies which cross-reacted with both dsDNA and the carboxyl terminal 22 amino acid peptide. Our results indicate that anti-dsDNA antibodies are heterogeneous and usually do not cross-react with the carboxyl terminal P peptide, but on occasion (1/10) a patient will produce anti-dsDNA antibodies cross-reactive with the carboxyl terminal P peptide.
Takeda
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I
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Rayno
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K
Wolfson-Reichlin
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M
Reichlin
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000915:Antibody Affinity; D002417:Cattle; D003429:Cross Reactions; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D012269:Ribosomal Proteins / Q000138:chemical synthesis / Q000276:immunology*
D000974:Antibodies, Antinuclear; C064601:L12E protein, Trypanosoma cruzi; D015800:Protozoan Proteins; D012269:Ribosomal Proteins