The use of yeast artificial chromosomes in transgenic animals: expression studies of the tyrosinase gene in transgenic mice.
Variegation and inherited somatic mosaicism has been observed in transgenic mice carrying yeast artificial chromosomes (YACs) in which a DNAse I hypersensitive site (HS) located -12 kb upstream of the mouse tyrosinase gene had been deleted. At present, we are generating new transgenic animals with minor deletions of the HS.
Giraldo
P
P
Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología, Madrid, Spain.
Giménez
E
E
Montoliu
L
L
D000818:Animals; D030801:Animals, Genetically Modified; D018244:Chromosomes, Artificial, Yeast; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D005813:Genes, Synthetic; D006200:Hair Color / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D014442:Monophenol Monooxygenase / Q000096:biosynthesis / Q000235:genetics*; D009030:Mosaicism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D017384:Sequence Deletion; D019076:Transgenes
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D014442:Monophenol Monooxygenase; D003850:Deoxyribonuclease I