LightCycler PCR assay for simultaneous detection of the H63D and S65C mutations in the HFE hemochromatosis gene based on opposite melting temperature shifts.
Bollhalder
M
M
Institute of Clinical Chemistry, University Hospital Zurich, Rämistrasse 100, CH-8091 Zürich, Switzerland.
Mura
C
C
Landt
O
O
Maly
F E
FE
D018076:DNA, Complementary / Q000737:chemistry; D005453:Fluorescence; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006432:Hemochromatosis / Q000235:genetics*; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins; D009154:Mutation; D009691:Nucleic Acid Denaturation; D015341:Nucleic Acid Probes; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D013696:Temperature; D013997:Time Factors
D018076:DNA, Complementary; C103673:HFE protein, human; D006680:HLA Antigens; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D015341:Nucleic Acid Probes