title: Probing the microenvironment of intracellular bacterial pathogens.
abstract: The identification of bacterial genes regulated in response to the intracellular environment is crucial to the understanding of host-pathogen interactions. Several techniques have been developed to identify and characterize bacterial genes that are induced during the intracellular infection and, potentially, may play a role in pathogenesis. This review discusses the strategies that have been utilized to examine differential gene expression by bacterial pathogens during the intracellular infection. Furthermore, a number of the differentially expressed genes are described.
authors.affiliation: Department of Microbiology and Immunology, University of Kentucky Chandler Medical Center, Lexington, KY 40536-0084, USA.
authors.forename: O S
authors.initials: OS
authors.lastname: Harb
authors.forename: Y
authors.initials: Y
authors.lastname: Abu Kwaik
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D010588:Phagosomes / Q000382:microbiology / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D014774:Virulence / Q000235:genetics