IS1626, a new IS900-related Mycobacterium avium insertion sequence.
An insertion sequence designated IS1626 was isolated and characterized from a Mycobacterium avium clinical strain. IS1626 was detected by high-stringency hybridization with the pMB22/S12 probe from IS900 of Mycobacterium paratuberculosis. IS1626 is 1418 bp in size and has a G+C content of 65 mol%. It has neither terminal inverted repeats nor flanking direct repeats. Analysis of three IS1626 insertion sites in the M. avium strain and the corresponding potential insertion sites in two IS1626-free M. avium strains indicated a consensus sequence of CATGCN(4-5)TCCTN(2)G for IS1626 insertion. In the three clones examined, IS1626 has the same orientation with respect to this target site. IS1626 has two major ORFs. ORF1179 encodes a predicted protein of 393 amino acids. ORF930, on the complementary strand of ORF1179, encodes a protein of 310 amino acids. The Shine-Dalgarno sequence for ORF930 is partially located in the flanking region, similar to other IS900-related elements. Analysis of the comparable features of insertion sequences and their variable occurrence in related organisms is useful for studying the evolution of these elements and their hosts.
Puyang
Xiaoling
X
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
Lee
Karen
K
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
Pawlichuk
Corey
C
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
Kunimoto
Dennis Y
DY
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H71.
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D016384:Consensus Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000235:genetics*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D004269:DNA, Bacterial