alpha(1,2)-fucosylation prevents sialyl Lewis x expression and E-selectin-mediated adhesion of fucosyltransferase VII-transfected cells.
E-selectin is a cytokine-inducible, calcium-dependent endothelial cell adhesion molecule that plays a critical role in the leucocyte-endothelium interaction during inflammation and is thought to contribute to the metastatic dissemination of tumour cells. Like the other selectins, E-selectin binds to ligands carrying the tetrasaccharide sialyl-Lewis x (NeuAcalpha2,3Galbeta1,4[Fucalpha1, 3]GlcNAc)1 or its isomer sialyl-Lewis a (NeuAcalpha2, 3Galbeta1, 3[Fucalpha1,4]GlcNAc). We examined the effect of expressing the H-type alpha(1,2)-fucosyltransferase or the alpha(2, 6)-sialyltransferase on the synthesis of sialyl-Lewis x by alpha(1, 3)fucosyltransferase. We found that H-type alpha(1, 2)-fucosyltransferase but not alpha(2,6)-sialyltransferase, strongly inhibited sialyl-Lewis x expression and E-selectin adhesion. We assume that H-type alpha(1,2)-fucosyltransferase competes with the endogenous alpha(2,3)-sialyltransferase for the N-acetyllactosamine structures assigned to further serve as acceptors for alpha(1, 3)fucosyltransferase.
Zerfaoui
M
M
INSERM Unité 260 faculté de médecine, Marseille, France.
Fukuda
M
M
Sbarra
V
V
Lombardo
D
D
El-Battari
A
A
D000606:Amino Sugars / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D002448:Cell Adhesion; D006224:Cricetinae; D019040:E-Selectin / Q000378:metabolism*; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis*; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D000606:Amino Sugars; D019040:E-Selectin; D005647:Fucosyltransferases; D005944:Glucosamine; D006023:Glycoproteins; D016256:Lewis X Antigen; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides; D012333:RNA, Messenger; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C032233:lactosamine; C031692:galactoside 3-fucosyltransferase