Dendritic cells require T cells for functional maturation in vivo.
We examined dendritic cell (DC) status in SCID and RAG2 -/- mice to assess the influence of T cells on DC development and function in vivo. These mice have reduced numbers of DC in the epidermis and lymph nodes draining hapten-sensitized skin. Epidermal DC in these mice were defective in presenting antigen in vivo to adoptively transferred, hapten-sensitized T cells from normal mice. Likewise, draining lymph node DC were deficient in their capacity to stimulate naive T cells in vitro and in vivo. DC numbers as well as the impaired ability to present antigen in vivo, were corrected by reconstituting these animals with normal T lymphocytes, suggesting that T cells are crucial for normal DC maturation and function in vivo.
Shreedhar
V
V
Department of Immunology, The University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Moodycliffe
A M
AM
Ullrich
S E
SE
Bucana
C
C
Kripke
M L
ML
Flores-Romo
L
L
D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation; D002452:Cell Count; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology*; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000473:pathology; D004817:Epidermis / Q000276:immunology / Q000473:pathology; D006241:Haptens / Q000276:immunology; D007801:Langerhans Cells / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D016176:T-Lymphocyte Subsets / Q000502:physiology* / Q000637:transplantation
D004268:DNA-Binding Proteins; D006241:Haptens; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2