title: Selective sensitivity of early postmitotic retinal cells to apoptosis induced by inhibition of protein synthesis.
abstract: In previous work we showed that apoptosis in retinal tissue from developing rats can be induced by inhibition of protein synthesis (Rehen et al. 1996, Development, 122, 1439-1448). Here we show that recent postmitotic cells are the cells sensitive to apoptosis triggered by blockade of protein synthesis. To label all proliferating cells in the retina, a series of injections of the nucleotide analogue, bromo-deoxy-uridine (BrdU, 60 mg/kg b.w.), was given in rat pups. Then, explants of the retina were incubated in vitro with the inhibitor of protein synthesis anisomycin (1.0-3.2 microg/mL) for 1 day to induce apoptosis. Detection of apoptotic bodies under differential interference contrast microscopy was combined with immunocytochemistry for BrdU, proliferating cell nuclear antigen (PCNA) or for various markers of retinal cell differentiation. Despite the large number of BrdU- and PCNA-labelled cells in the tissue, the vast majority of the cells that underwent apoptosis were postmitotic cells which have left the mitotic cycle 3-4 days before. However, these cells were not labelled with antibodies to calretinin, calbindin, rhodopsin or to a Muller glial cell marker, suggesting that these are early postmitotic neurons. We suggest that during migration and initial differentiation, the apoptotic machinery is blocked by suppressor proteins, thus allowing recent postmitotic cells to find their final positions and differentiate while protected from apoptosis.
authors.affiliation: Instituto de Biofisica Carlos Chagas Filho, UFRJ, Centro de Ciencias daSaude, Rio de Janeiro, Brasil.
authors.forename: S K
authors.initials: SK
authors.lastname: Rehen
authors.forename: D D
authors.initials: DD
authors.lastname: Neves
authors.forename: L
authors.initials: L
authors.lastname: Fragel-Madeira
authors.forename: L R
authors.initials: LR
authors.lastname: Britto
authors.forename: R
authors.initials: R
authors.lastname: Linden
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D017209:Apoptosis; D001973:Bromodeoxyuridine; D064032:Calbindin 2; D064026:Calbindins; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008938:Mitosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D064026:Calbindins; D018809:Proliferating Cell Nuclear Antigen; D011500:Protein Synthesis Inhibitors; D064030:S100 Calcium Binding Protein G; D000841:Anisomycin; D012243:Rhodopsin; D001973:Bromodeoxyuridine