title: Factors important for fusogenic activity of peptides: molecular modeling study of analogs of fusion peptide of influenza virus hemagglutinin.
abstract: Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific structural and/or hydrophobic properties inherent in peptides with fusogenic activity. It was shown that, regardless of characteristics common to all analogs (peripheral disposition at the water-lipid interface, amphiphilic nature, alpha-helical structure, etc.), only fusion active peptides reveal a specific 'tilted oblique-oriented' pattern of hydrophobicity on their surfaces and a certain depth of penetration to the non-polar membrane core. The conclusion was reached that these factors are among the most important for the specific destabilization of a bilayer, which is followed by membrane fusion.
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ul. Miklukho-Maklaya, 16/10, V-437, 117871 GSP, Moscow, Russia. efremov@nmr.ru
authors.forename: R G
authors.initials: RG
authors.lastname: Efremov
authors.forename: D E
authors.initials: DE
authors.lastname: Nolde
authors.forename: P E
authors.initials: PE
authors.lastname: Volynsky
authors.forename: A A
authors.initials: AA
authors.lastname: Chernyavsky
authors.forename: P V
authors.initials: PV
authors.lastname: Dubovskii
authors.forename: A S
authors.initials: AS
authors.lastname: Arseniev
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008561:Membrane Fusion; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D009975:Orthomyxoviridae / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D010455:Peptides; D011993:Recombinant Fusion Proteins