Comparison of A68 levels in Alzheimer diseased and non-Alzheimer's diseased brain by two ALZ50 based methods.
A total of 61 human brain specimens were analyzed with both ELISA and Western Blot using the ALZ50 monoclonal antibody. The brain specimens included: Alzheimer's Disease (AD, n=31), AD/Down's (n=2), Normal (n=14), Parkinson's Disease (n=7), Huntington's chorea (n=2), Wernicke-Korsakov's Encephalopathy (n=3), and Motor Neuron Disease (n=2). The non-AD cases (n=28) had no detectable A68 by ELISA, and showed no A68 bands by Western blot. The AD cases (n=33), all were positive for A68 by the ELISA, but only 31 of 33 had visible A68 band by Western blot. Additionally, a method for solubilization of A68 is reported.
Miller
B E
BE
Department of Psychiatry & Behavioral Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0421, USA. millerb@etsu.edu
D000544:Alzheimer Disease / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D016875:tau Proteins
C056167:Alzheimer's disease antigen; D000911:Antibodies, Monoclonal; D000941:Antigens; C054369:MAPT protein, human; D009419:Nerve Tissue Proteins; D016875:tau Proteins