title: Magnetic resonance imaging in the evaluation of inflammatory lesions in muscular and soft tissues: an experimental infection model induced by Candida albicans.
abstract: We have developed an experimental model to monitor inflammatory lesions in muscle and soft-tissues during the different stages of the disease by means of Magnetic Resonance Imaging (MRI). MRI of mice legs infected with Candida albicans was performed by standard two-dimensional spin echo and fast spin echo (RARE) using customized coils. The MRI findings were compared with pathologic examinations at the initial acute and established acute inflammatory stages, which provided accurate and detailed information on the evolution of the processes involved. The yeast caused inflammation within the first hours post-inoculation, appearing on T2-weighted images as an inhomogeneous mass with increased signal intensity. The presence of fungal hyphae was observed as hypointense signal areas in both T2 and T1 weighted images, with histologic confirmation. Areas of decreased signal intensity on T2 weighted images were apparent on the last experimental day and were attributed to the granulation tissue located within the capsule surrounding the abscess. The close correlation found between MRI and histopathology suggests that MRI is an ideal radiologic technique for monitoring the clinical and therapeutic follow-up of fungal infections in muscle and soft tissues.
authors.affiliation: Department of Chemistry-Physics II, Faculty of Pharmacy, San Carlos Clinic Hospital, Complutense University, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Cabello
authors.forename: B
authors.initials: B
authors.lastname: Carrero-González
authors.forename: P
authors.initials: P
authors.lastname: Avilés
authors.forename: C
authors.initials: C
authors.lastname: Santisteban
authors.forename: R J
authors.initials: RJ
authors.lastname: Méndez
authors.forename: J
authors.initials: J
authors.lastname: Ferreirós
authors.forename: N
authors.initials: N
authors.lastname: Malpica
authors.forename: A
authors.initials: A
authors.lastname: Santos
authors.forename: D
authors.initials: D
authors.lastname: Gargallo-Viola
authors.forename: J
authors.initials: J
authors.lastname: Regadera
mesh_terms: D000818:Animals; D002176:Candida albicans; D002177:Candidiasis / Q000473:pathology*; D003141:Communicable Diseases / Q000382:microbiology; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009135:Muscular Diseases / Q000382:microbiology / Q000473:pathology*; D018461:Soft Tissue Infections / Q000382:microbiology / Q000473:pathology*; D013848:Thigh / Q000382:microbiology / Q000473:pathology; D013997:Time Factors