title: Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina.
abstract: P84 and integrin associated protein (IAP) are heterophilic binding partners that are expressed in the central nervous system in addition to a variety of other tissues. Both molecules are known to be involved in cell signaling in nonneural tissues. In the retina, both molecules are expressed prominently in plexiform layers, suggesting a possible association with synapses. Here, we examined the cellular expression and ultrastructural localization of the two molecules in the developing mouse retina. Both appeared to be expressed at one or both sides of synaptic sites, although the expression of IAP in the retina precedes that of P84. Examination of transgenic IAP-null retinae revealed a failure of P84 to become associated with synaptic sites, suggesting the interaction of P84 with IAP was necessary for P84's synaptic localization. These findings suggest that the signaling activities of P84 and IAP are localized to sites of synaptic contact in the retina. Thus this pair of synapse-associated molecules represents a bidirectional signaling system that could function to modify synaptic activity or possibly trophic interactions between central neurons.
authors.affiliation: Department of Neurobiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Mi
authors.forename: P
authors.initials: P
authors.lastname: Jiang
authors.forename: W L
authors.initials: WL
authors.lastname: Weng
authors.forename: F P
authors.initials: FP
authors.lastname: Lindberg
authors.forename: V
authors.initials: V
authors.lastname: Narayanan
authors.forename: C F
authors.initials: CF
authors.lastname: Lagenaur
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation; D051928:CD47 Antigen; D002352:Carrier Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure*; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000235:genetics*; D010786:Photoreceptor Cells / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic; D012160:Retina / Q000378:metabolism* / Q000648:ultrastructure*; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D051928:CD47 Antigen; D002352:Carrier Proteins; C497895:Cd47 protein, mouse; D008562:Membrane Glycoproteins; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger; D011971:Receptors, Immunologic