title: Annexins VII and XI are present in a human macrophage-like cell line. Differential translocation on FcR-mediated phagocytosis.
abstract: We have studied the divalent cation-dependent association of proteins to subcellular fractions of human macrophage-like cells before and after FcR-mediated phagocytosis. Among these proteins we have identified annexins VII and XI for the first time in these cells, along with annexins I, III, and VI. Although all of these annexins are present in the cytosolic fraction, the extent of their association to membrane and phagosome fractions from resting and stimulated cells is variable. Annexin VII translocates from cytosolic to membrane fractions after phagocytic stimulation, along with annexin I, III, and VI. Annexins VII and XI are found associated with purified phagosomes along with I, III, and VI, and this association is greater after a 24-h chase period. Our results show differences in the intracellular distribution of different annexins in macrophage-like cells on phagocytosis. Annexins VII, VI, III, and I respond to particle ingestion by translocating to phagosomes and other cell membrane structures, whereas annexin XI translocates predominantly to phagosomes, suggesting dissimilarities in their function.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Area di Ricerca, Trieste, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Pittis
authors.forename: R C
authors.initials: RC
authors.lastname: Garcia
mesh_terms: D017310:Annexin A7 / Q000378:metabolism*; D017302:Annexins / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002474:Cell-Free System; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology; D010587:Phagocytosis / Q000502:physiology*; D010588:Phagosomes; D011506:Proteins / Q000378:metabolism; D011961:Receptors, Fc / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D017310:Annexin A7; D017302:Annexins; D007476:Ionophores; D008934:Mitogens; D011506:Proteins; D011961:Receptors, Fc; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium