PCR-based diagnosis of the Filipino (--(FIL)) and Thai (--(THAI)) alpha-thalassemia-1 deletions.
In southeast Asia, the carrier frequency of two-gene alpha-thalassemia deletions is quite high, ranging from 4% to 14% depending on the population. The most common alpha-thalassemia-1 deletion is the so-called southeast Asian deletion (--(SEA)). In addition, a significant proportion of cases involve two other deletions, the Filipino (--(FIL)) and Thai (--(THAI)) deletions. In this report, we identify the deletion breakpoints for the (--(FIL)) and (--(THAI)) deletions, and describe PCR-based protocols for rapid and reliable DNA diagnosis of these deletions.
Eng
B
B
Provincial Hemoglobinopathy DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton Health Sciences Corporation, Hamilton, Ontario, Canada.
Patterson
M
M
Borys
S
S
Chui
D H
DH
Waye
J S
JS
D001210:Asia, Southeastern / Q000208:ethnology; D001483:Base Sequence; D017353:Gene Deletion; D005914:Globins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009864:Ontario; D010679:Philippines / Q000208:ethnology; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013785:Thailand / Q000208:ethnology; D017085:alpha-Thalassemia / Q000175:diagnosis / Q000235:genetics*
D005914:Globins