title: Production of 10-ketostearic acid and 10-hydroxystearic acid by strains of Sphingobacterium thalpophilum isolated from composted manure.
abstract: Six strains of Sphingobacterium thalpophilum were isolated from a compost mixture enriched with oleic acid. These strains converted oleic acid to 10-ketostearic acid (10-KSA; 87-94% of the total conversion product) and to 10-hydroxystearic acid (10-HSA; 6-13%) exhibiting three levels of total product yields. The predominant production of 10-KSA by these new S. thalpophilum isolates is in contrast to strain 142b (NRRL B-14797) previously isolated from a commercial compost, which produces exclusively 10-HSA. The production yield of greater than 75% 10-KSA was achieved in 36 h, acting on 0.26 g of oleic acid in 30-ml fermentation broth incubated with agitation at 28 degrees C. For easy maintenance, fast-growth, and high bioreactivity, these S. thalpophilum strains are suited for developing a large-scale production of 10-KSA and 10-HSA.
authors.affiliation: Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Kuo
authors.forename: A C
authors.initials: AC
authors.lastname: Lanser
authors.forename: L K
authors.initials: LK
authors.lastname: Nakamura
authors.forename: C T
authors.initials: CT
authors.lastname: Hou
mesh_terms: D001431:Bacteriological Techniques; D019414:Chlorobi / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D008372:Manure / Q000382:microbiology*; D013058:Mass Spectrometry; D019301:Oleic Acid / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013229:Stearic Acids / Q000378:metabolism*
chemical_list: C403718:10-ketostearic acid; D008372:Manure; D013229:Stearic Acids; D019301:Oleic Acid; C070376:10-hydroxystearic acid