Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas hydrophila.
The newly screened Aeromonas hydrophila produces copolymer consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). The characteristics of cell growth and polymer accumulation were examined using various carbon sources. P(3HB-co-3HHx) was produced from lauric acid and oleic acid only. P(3HB-co-3HHx) content can be increased by limitation of phosphorus. A maximal P(3HB-co-3HHx) content of 28.8 wt% could be obtained in flask culture. By applying the optimally designed nutrient feeding strategy, cell dry weight, P(3HB-co-3HHx) content, and 3HHx fraction obtained over the course of 43 h were 95.7 g/L, 45.2 wt%, and 17 mol%, respectively, resulting in a productivity of 1.01 g polyhydroxyalkanoate (PHA)/L. h.
Lee
S H
SH
Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea.
Oh
D H
DH
Ahn
W S
WS
Lee
Y
Y
Choi
J i
Ji
Lee
S Y
SY
D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D016980:Aeromonas hydrophila / Q000254:growth & development* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002208:Caproates / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002455:Cell Division; D005285:Fermentation; D007850:Lauric Acids / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D002208:Caproates; D002244:Carbon; D007850:Lauric Acids; D019301:Oleic Acid; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C030358:lauric acid