title: Differentiation of Lactobacillus isolates from infant faeces by SDS-PAGE and rRNA-targeted oligonucleotide probes.
abstract: Isolates of lactobacilli from infant faeces phenotypically characterized as Lactobacillus paracasei subsp. paracasei (six strains), Lact. rhamnosus (six strains), Lact. gasseri (three strains), Lact. acidophilus (one strain) and Lact. fermentum/reuteri (three strains) according to recent classification systems were subjected to SDS-PAGE of whole cell proteins and rRNA-targeted oligonucleotide probe hybridization, in order to confirm the phenotypic characterization and elucidate the exact taxonomic position of the three strains that had properties between fermentum and reuteri. Results suggested a good agreement between the phenotypic characterization, SDS-PAGE and rRNA-targeted oligonucleotide probe hybridization for strains of all species except for the Lact. fermentum/reuteri strains. Results obtained by rRNA probes suggested a possible phylogenetic relatedness of the strains to Lact. reuteri. Isolates from infant faeces with interesting probiotic properties could be used as components of fermented milk products.
authors.affiliation: Laboratory of Food Microbiology and Hygiene, Faculty of Agriculture, Aristotle University of Thessaloniki, Greece.
authors.forename: V
authors.initials: V
authors.lastname: Xanthopoulos
authors.forename: I
authors.initials: I
authors.lastname: Ztaliou
authors.forename: W
authors.initials: W
authors.lastname: Gaier
authors.forename: N
authors.initials: N
authors.lastname: Tzanetakis
authors.forename: E
authors.initials: E
authors.lastname: Litopoulou-Tzanetaki
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D007778:Lactobacillus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal