Effects of intrathecal morphine, clonidine and baclofen on allodynia after partial sciatic nerve injury in the rat.
BACKGROUND
Increased response to mechanical or cold stimulation of hind paws was observed in rats with partial sciatic nerve injury as a result of photochemically induced ischemia. The present study examined the effects of intrathecal morphine, clonidine and baclofen on the allodynia-like responses.


METHODS
The left sciatic nerves of rats were irradiated for 2 min with an argon ion laser under chloral hydrate anesthesia. The threshold of paw withdrawal to mechanical stimulation was determined with a series of monofilaments (von Frey hairs). The response to cold stimulation was tested by spraying ethyl chloride on the plantar surface of the paw. When rats were exhibiting stable mechanical and cold allodynia-like behaviors after nerve injury, the effects of i.t. morphine (1, 2, 7 microg), clonidine (1, 2, 7 microg) and baclofen (0.1, 0.2, 0.7, 9 microg) in a cumulative dose regime were investigated.


RESULTS
Intrathecal morphine dose-dependently alleviated the mechanical and cold allodynia without inducing motor impairment or sedation. Intrathecal clonidine did not alter the response of hind paws to mechanical stimulation, but reduced the cold allodynia. Intrathecal baclofen reduced the responses of rats to mechanical stimulation only at doses that also induced profound motor deficits.


CONCLUSIONS
The present data suggest that intrathecal morphine, and to some extent clonidine, but not baclofen, alleviated the abnormal pain-related behaviors in this new rat model of partial peripheral nerve injury. Differences in the pharmacological profile between the present model and other models of peripheral nerve injury are discussed.
Hao
J X
JX
Karolinska Institute, Department of Medical Laboratory Sciences and Technology, Huddinge University Hospital, Sweden.
Xu
I S
IS
Xu
X J
XJ
Wiesenfeld-Hallin
Z
Z
D000700:Analgesics / Q000008:administration & dosage*; D000701:Analgesics, Opioid / Q000008:administration & dosage; D000818:Animals; D001418:Baclofen / Q000008:administration & dosage*; D003000:Clonidine / Q000008:administration & dosage*; D003080:Cold Temperature; D004305:Dose-Response Relationship, Drug; D018755:GABA Agonists / Q000008:administration & dosage*; D007278:Injections, Spinal; D008297:Male; D009020:Morphine / Q000008:administration & dosage*; D010146:Pain / Q000188:drug therapy* / Q000209:etiology; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries*
D000700:Analgesics; D000701:Analgesics, Opioid; D018755:GABA Agonists; D009020:Morphine; D001418:Baclofen; D003000:Clonidine