title: Molecular phylogenetic analysis of archaeal intron-containing genes coding for rRNA obtained from a deep-subsurface geothermal water pool.
abstract: Molecular phylogenetic analysis of a naturally occurring microbial community in a deep-subsurface geothermal environment indicated that the phylogenetic diversity of the microbial population in the environment was extremely limited and that only hyperthermophilic archaeal members closely related to Pyrobaculum were present. All archaeal ribosomal DNA sequences contained intron-like sequences, some of which had open reading frames with repeated homing-endonuclease motifs. The sequence similarity analysis and the phylogenetic analysis of these homing endonucleases suggested the possible phylogenetic relationship among archaeal rRNA-encoded homing endonucleases.
authors.affiliation: Deep-sea Microorganisms Research Group, Japan Marine Science & Technology Center, Yokosuka 237-0061, Japan. Ken.Takai@pnl.gov
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: K
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D006358:Hot Temperature; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012335:RNA, Ribosomal