Generation of a monoclonal antibody that recognizes a polymorphic epitope of C57BL/6 Ly-49G2.
To facilitate the study of natural killer (NK) cell functions, we have generated a panel of hybridomas that secrete antibodies recognizing B6 NK cell surface markers. One of these hybridomas, Cwy-3, secretes a mouse IgG1 specific for the B6 allele of Ly-49G2 (Ly-49G2B6). In addition, this monoclonal antibody (MAb) fails to stain A-LAKs derived from all other mouse strains tested. This suggests that Cwy-3 recognizes a polymorphic epitope of Ly-49G2B6. More importantly, this MAb exhibits no cross-reactivity to other known B6 Ly-49 family members. Therefore, Cwy-3 is in sharp contrast to the two known anti-Ly-49G2 MAbs, which are reported to recognize a nonpolymorphic epitope of Ly-49G2, and react with other Ly-49 members. In this regard, Cwy-3 will offer significant advantages over the cross-reactive anti-Ly-49G2 MAbs in fully defining the specificity and function of the Ly-49G2B6 NK cell receptor. With this novel Ly-49G2-specific MAb, we have isolated an EL-4 subline expressing a significant density of Ly-49G2 receptors.
Chang
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CS
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Silver
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Kane
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000950:Antigens, Ly; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008814:Mice, Inbred NZB; D018345:Mice, Knockout; D055630:Receptors, NK Cell Lectin-Like
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000939:Epitopes; D018398:Homeodomain Proteins; D007074:Immunoglobulin G; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D055630:Receptors, NK Cell Lectin-Like; C064658:RAG-1 protein