title: Concurrent administration of the erythromycin breath test (EBT) and oral midazolam as in vivo probes for CYP3A activity.
abstract: Given the prominent role of CYP3A in the metabolism of drugs, it is important to identify whether new chemical entities will affect this enzyme system and produce clinically relevant drug interactions. This study evaluated concomitant administration of intravenous [14C N-methyl] erythromycin (3 microCi) (erythromycin breath test; EBT) and 2 mg oral midazolam as probes of systemic and of systemic plus presystemic CYP3A activity, respectively. Twelve males received the probes in a two-period crossover fashion: one period included the probes on two occasions, 5 days apart; in the second period, 200 mg ketoconazole was given orally 2 hours prior to the probes. The within-subject CV for EBT (%14CO2/h) and midazolam AUC0-last was 4.9% and 16.9%, respectively. Ketoconazole reduced %14CO2/h by 43% and increased midazolam AUC0-last by approximately fivefold. In a nonrandomized third period (N = 5), ketoconazole was given simultaneously with midazolam (no EBT); midazolam AUC0-last was similar whether ketoconazole was given 2 hours prior to or simultaneously with the midazolam. The low midazolam dose was generally well tolerated; mild sedation was occasionally seen. Concurrent administration of the EBT and oral midazolam is a sensitive and reproducible tool to screen new chemical entities for potentially important CYP3A interactions.
authors.affiliation: Merck Research Laboratories, West Point, Pennsylvania, USA.
authors.forename: J
authors.initials: J
authors.lastname: McCrea
authors.forename: T
authors.initials: T
authors.lastname: Prueksaritanont
authors.forename: B J
authors.initials: BJ
authors.lastname: Gertz
authors.forename: A
authors.initials: A
authors.lastname: Carides
authors.forename: L
authors.initials: L
authors.lastname: Gillen
authors.forename: S
authors.initials: S
authors.lastname: Antonello
authors.forename: M J
authors.initials: MJ
authors.lastname: Brucker
authors.forename: C
authors.initials: C
authors.lastname: Miller-Stein
authors.forename: B
authors.initials: B
authors.lastname: Osborne
authors.forename: S
authors.initials: S
authors.lastname: Waldman
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001189:Aryl Hydrocarbon Hydroxylases; D001944:Breath Tests / Q000379:methods*; D018592:Cross-Over Studies; D051544:Cytochrome P-450 CYP3A; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000009:adverse effects / Q000494:pharmacology; D004917:Erythromycin / Q000378:metabolism*; D006801:Humans; D007654:Ketoconazole / Q000009:adverse effects / Q000494:pharmacology; D008297:Male; D008874:Midazolam / Q000009:adverse effects / Q000378:metabolism*; D010089:Oxidoreductases, N-Demethylating / Q000032:analysis* / Q000037:antagonists & inhibitors; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D004917:Erythromycin; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D008874:Midazolam; D007654:Ketoconazole