[Genetic analysis of leaf-parent-type plants regenerated from somatic hybridization in Citrus].
The leaf indexes and stoma characteristics of leaf-parent-type regenerants were the same as those of the leaf parent Rough lemon, but quite different from the somatic hybrids. Leaf-parent-type plants were diploid (2n = 2x = 18). Patterns of POX, PPO, GOT isozymes were identical to those of Rough lemon. The plants were analyzed by using 54 arbitrary primers with polymorphisms. Most of the primers (52) showed that leaf-parent-type plants were in concordance with Rough lemon. However, with primer OPW-12, the plants contained a unique band of Hamlin sweet orange. Amplification products with primer OPV-04 showed slight differences among the accessions. The research suggested that most genetic constitutions of the leaf-parent-type plants were from leaf parent Rough lemon and a little from embryogenic suspension parent Hamlin sweet orange.
Shi
Y Z
YZ
State Key Laboratory in Genetic Improvement of Crops and Citrus, Research Institute Huazhong, Agricultural University, Wuhan.
Deng
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XX
D002957:Citrus / Q000235:genetics*; D006824:Hybridization, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012038:Regeneration