Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples.
We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.
Makino
S
S
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Kurazono
H
H
Chongsanguam
M
M
Hayashi
H
H
Cheun
H
H
Suzuki
S
S
Shirahata
T
T
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D004731:Endotoxins / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005513:Food Inspection / Q000379:methods*; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology; D011634:Public Health; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012478:Salmonella Food Poisoning / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D004731:Endotoxins; D004768:Enterotoxins; C027952:salmonella toxin