title: Removal of mercury from chloralkali electrolysis wastewater by a mercury-resistant Pseudomonas putida strain.
abstract: A mercury-resistant bacterial strain which is able to reduce ionic mercury to metallic mercury was used to remediate in laboratory columns mercury-containing wastewater produced during electrolytic production of chlorine. Factory effluents from several chloralkali plants in Europe were analyzed, and these effluents contained total mercury concentrations between 1.6 and 7.6 mg/liter and high chloride concentrations (up to 25 g/liter) and had pH values which were either acidic (pH 2.4) or alkaline (pH 13.0). A mercury-resistant bacterial strain, Pseudomonas putida Spi3, was isolated from polluted river sediments. Biofilms of P. putida Spi3 were grown on porous carrier material in laboratory column bioreactors. The bioreactors were continuously fed with sterile synthetic model wastewater or nonsterile, neutralized, aerated chloralkali wastewater. We found that sodium chloride concentrations up to 24 g/liter did not inhibit microbial mercury retention and that mercury concentrations up to 7 mg/liter could be treated with the bacterial biofilm with no loss of activity. When wastewater samples from three different chloralkali plants in Europe were used, levels of mercury retention efficiency between 90 and 98% were obtained. Thus, microbial mercury removal is a potential biological treatment for chloralkali electrolysis wastewater.
authors.affiliation: Division of Microbiology, National Research Center for Biotechnology (GBF), D-38124 Braunschweig, Germany.
authors.forename: H
authors.initials: H
authors.lastname: von Canstein
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: K N
authors.initials: KN
authors.lastname: Timmis
authors.forename: W D
authors.initials: WD
authors.lastname: Deckwer
authors.forename: I
authors.initials: I
authors.lastname: Wagner-Döbler
mesh_terms: D001682:Biological Availability; D019149:Bioreactors; D002712:Chlorides; D004352:Drug Resistance, Microbial; D004572:Electrolysis; D005618:Fresh Water; D019015:Geologic Sediments; D008628:Mercury / Q000493:pharmacokinetics* / Q000494:pharmacology; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D014865:Waste Disposal, Fluid; D014871:Water Microbiology; D014877:Water Pollution, Chemical
chemical_list: D002712:Chlorides; D008628:Mercury