Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis.
The design, fabrication, and operation of a radial capillary array electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10-cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detection channels. Loading of 96 samples in parallel is achieved using a pressurized capillary array system. High-quality separations of 96 pBR322 restriction digest samples are achieved in < 120 s with the microplate system. The practical utility and multicolor detection capability is demonstrated by analyzing 96 methylenetetrahydrofolate reductase (MTHFR) alleles in parallel using a noncovalent 2-color staining method. This work establishes the feasibility of performing high-throughput genotyping separations with capillary array electrophoresis microplates.
Shi
Y
Y
Chemistry Department, University of California, Berkeley 94720, USA.
Simpson
P C
PC
Scherer
J R
JR
Wexler
D
D
Skibola
C
C
Smith
M T
MT
Mathies
R A
RA
D019075:Electrophoresis, Capillary / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*
D009696:Nucleic Acids