[A rapid determination method for scopolia extract in gastrointestinal drugs by capillary electrophoresis].
A rapid and simple determination of tropane-alkaloids (hyoscyamine, scopolamine) in gastrointestinal drugs was investigated by capillary electrophoresis. Micellar electrokinetic capillary chromatography (MEKC) using a fused silica capillary (560 mm x 0.075 mm i.d.) in 0.1 M SDS 20 mM borate buffer (pH 10)/acetonitrile (97:3) gave complete separation of the two alkaloids within 40 min. A sample solution was introduced by pressure method (50 mbar, 3.7 s), separation conditions applied voltage 15 kV and on-column detection was performed at 210 nm. Calibration curves for hyoscyamine and scopolamine showed a good linearity in the range of 4-12 micrograms/ml (r = 0.9970) and 390-1150 ng/ml (r = 0.9976). The present method is applicable to the simple and rapid determination of hyoscyamine and scopolamine in commercial gastrointestinal drugs.
Ori
K
K
Healthcare Research Laboratories, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Mikata
H
H
Tsurumori
T
T
D001285:Atropine / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary; D019075:Electrophoresis, Capillary / Q000379:methods*; D005765:Gastrointestinal Agents / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012601:Scopolamine / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D019657:Solanaceae / Q000737:chemistry*
D005765:Gastrointestinal Agents; D010936:Plant Extracts; D012967:Sodium Dodecyl Sulfate; D001285:Atropine; D012601:Scopolamine