Capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus. Structural study of an undecasaccharide obtained by lithium degradation.
The capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus has been subjected to a specific degradation with lithium in ethylenediamine. The released undecasaccharide attached to one unit of tetrahydroxycyclopentanecarboxylic acid has been characterized by a combination of 2D nuclear magnetic resonance spectroscopy, mass spectrometry, monosaccharidic composition and linkage analyses. From the overlap of the structure of this oligosaccharide with previously identified di-, tri- and pentasaccharides released by mild acid hydrolysis, the capsular polysaccharide was deduced to have a pentadecasaccharide repeating unit with the following structure:
Gloaguen
V
V
Equipe de Blycobiologie Végétale, Institut de Biotechnologie, Université de Limoges, France. vgloaguen@unilim.fr
Morvan
H
H
Hoffmann
L
L
Plancke
Y
Y
Wieruszeski
J M
JM
Lippens
G
G
Strecker
G
G
D002240:Carbohydrate Sequence; D000458:Cyanobacteria / Q000737:chemistry*; D005029:Ethylenediamines; D008094:Lithium; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D005029:Ethylenediamines; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C031234:ethylenediamine; D008094:Lithium