Comparative chromosome painting between marsupial orders: relationships with a 2n = 14 ancestral marsupial karyotype.
A 2n = 14 karyotype is shared by some species in each of the marsupial orders in Australian and American superfamilies, suggesting that the ancestral marsupial chromosome complement was 2n = 14. We have used chromosome painting between distantly related marsupial species to discover whether genome arrangements in 2n = 14 species in two Australian orders support this hypothesis. Cross-species chromosome painting was used to investigate chromosome rearrangements between a macropodid species Macropus eugenii (2n = 16) and a wombat species in a different suborder (Lasiorhinus latifrons, 2n = 14), and a dasyurid species in a different order (Sminthopsis macroura, 2n = 14). We demonstrate that many chromosome regions are conserved between all three species, and deduce how the similar 2n = 14 karyotypes of species in the two orders are related to a common ancestral 2n = 14 karyotype.
De Leo
A A
AA
Department of Biochemistry and Genetics, La Trobe University, Bundoora, VIC, Australia.
Guedelha
N
N
Toder
R
R
Voullaire
L
L
Ferguson-Smith
M A
MA
O'Brien
P C
PC
Graves
J A
JA
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D020223:Chromosome Painting; D017931:DNA Primers; D007621:Karyotyping; D008394:Marsupialia / Q000235:genetics*; D013045:Species Specificity; D014960:X Chromosome; D014998:Y Chromosome
D017931:DNA Primers