title: Transient increase of a protein kinase activity identified to CK2 during sea urchin development.
abstract: Using GST-EF-1 delta as an exogenous substrate, and EF-1 delta kinase activity was shown to increase transiently during early development of sea urchin embryos. The basal activity of EF-1 delta kinase in unfertilized eggs was 150 fmoles/min/mg protein. The activity began to increase 10 h after fertilization and reached its maximum level (8.4 x basal) at 24 h. The activity then declined to twice the basal value at 72 h post-fertilization. The EF-1 delta kinase activity was identified to a CK2-type enzyme on the basis of its substrate specificity for EF-1 delta, crude casein and beta casein, its inhibition by heparin, DRB, 2,3-bisphosphoglycerate, and its stimulation by spermine, spermidine, and polylysin. Furthermore, the activity was inhibited by the synthetic peptide RRREEETEEE specific for CK2. DRB (200 microM) and 2,3-bisphosphoglycerate (2.5 mM) blocked or delayed the transition from blastula to gastrula of the embryos, suggesting a role for the kinase in early development.
authors.affiliation: Université Pierre et Marie Curie (UFR 937), Institut National des Sciences de l'Univers (INSU), BP 74, Roscoff Cedex, 29682, France.
authors.forename: C
authors.initials: C
authors.lastname: Delalande
authors.forename: R
authors.initials: R
authors.lastname: Bellé
authors.forename: P
authors.initials: P
authors.lastname: Cormier
authors.forename: O
authors.initials: O
authors.lastname: Mulner-Lorillon
mesh_terms: D000818:Animals; D047390:Casein Kinase II; D002364:Caseins / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009865:Oocytes / Q000201:enzymology; D020648:Peptide Elongation Factor 1 / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012098:Reproduction; D012617:Sea Urchins / Q000196:embryology*; D013997:Time Factors
chemical_list: D002364:Caseins; D004791:Enzyme Inhibitors; D020648:Peptide Elongation Factor 1; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases