title: The structure of the specific capsular polysaccharide of Rhodococcus equi serotype 4.
abstract: The specific capsular polysaccharide produced by Rhodococcus equi serotype 4 was found to be a high-molecular-weight acidic polymer composed of D-glucose, D-mannose, pyruvic acid and a previously unidentified 5-amino-3,5-dideoxynonulosonic (rhodaminic) acid in the proportions 2:1:1:1. Structural analysis, employing a combination of microanalytical methods, nuclear magnetic resonance spectroscopy, and mass spectrometric techniques, established that the polysaccharide consisted of linear repeating tetrasaccharide units having the sequence of residues shown below. In the native polysaccharide, the rhodaminic acid residues were present as their acetamido derivatives (RhoANAc) and carried 1-carboxyethylidene groups that bridged the O-7 and O-9 positions. Treatment of the capsular polysaccharide with dilute acetic acid and/or anhydrous hydrogen fluoride under hydrolytic/solvolytic conditions, resulted in the formation of four different oligosaccharide species. The 1H and 13C NMR resonances of these oligosaccharide fragments and of the native serotype 4 capsular polysaccharides were fully assigned by homo- and heteronuclear chemical shift correlation methods.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada. severnw@agresearch.cri.nz
authors.forename: W B
authors.initials: WB
authors.lastname: Severn
authors.forename: J C
authors.initials: JC
authors.lastname: Richards
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D016929:Rhodococcus equi / Q000737:chemistry*; D012703:Serotyping; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D011134:Polysaccharides; D011135:Polysaccharides, Bacterial