A study of dextran production from maltodextrin by cell suspensions of Gluconobacter oxydans NCIB 4943.
This study investigated dextran synthesis from a commercial maltodextrin substrate using cell suspensions of G. oxydans NCIB 4943 as catalysts. Experiments were arranged according to a central composite statistical design. The effects of substrate concentration (10-100 g l-1), cell concentration (0.32-32.0 g wet weight l-1), time of reaction (8-48 h) and pH (3.5-5.5), each at three levels, on dextran yield and dextran molecular weight (MW), were investigated. Response surface methodology was used to assess factor interactions, and empirical models describing the two responses were fitted. Most of the variance in dextran yield could be explained by the fitted model (R2 = 0.96). Dextran yield ranged from 1.21 to 41.69%. The presence of significant negative quadratic effects of cell concentration and time indicated that dextran yield reached a plateau and thus, optimum levels of cell concentration and time could be identified to maximize dextran yield. Dextran MW ranged from 6.6 to 38 kDa and was characterized by the significant interactions of reaction time with substrate concentration and cell concentration. The model, however, could account for only 60% of the variance in dextran MW. Possible reasons for this are discussed.
Mountzouris
K C
KC
Department of Food Science and Technology, University of Reading, Norwich, UK.
Gilmour
S G
SG
Jay
A J
AJ
Rastall
R A
RA
D003470:Culture Media; D003911:Dextrans / Q000096:biosynthesis*; D005285:Fermentation; D020600:Gluconobacter oxydans / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008745:Methylation; D011134:Polysaccharides / Q000378:metabolism*; D013394:Sucrase-Isomaltase Complex
D003470:Culture Media; D003911:Dextrans; D011134:Polysaccharides; C008315:maltodextrin; D005964:Glucosyltransferases; C402863:dextrin dextranase; D013394:Sucrase-Isomaltase Complex