A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions.
A system for studying the early growth response of Streptococcus gordonii to environmental stimuli has been developed. A reporter gene, encoding alpha-amylase, has been integrated into an rRNA operon to monitor changes in cellular physiology associated with the initiation of growth. Two such strains with single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to growth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rrn operons containing the reporter gene suggests that they are differentially expressed over the course of growth.
Natarajan
A
A
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455, USA.
Everard
M
M
Manias
D
D
Srienc
F
F
Dunny
G
G
D019677:Artificial Gene Fusion; D001431:Bacteriological Techniques; D017930:Genes, Reporter / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012680:Sensitivity and Specificity; D013291:Streptococcus / Q000235:genetics / Q000254:growth & development*; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism; D012407:rRNA Operon / Q000235:genetics*
D000516:alpha-Amylases