title: [Detection and prevalence of verotoxin-producing Escherichia coli (VTEC) in raw sausage].
abstract: We investigated 158 samples of shortly ripened raw sausages bought in supermarkets of Dessau within 4 month. In 14 (8.8%) samples Verotoxin-producing E. coli were detected. 13 VT-positive samples were found in the group of easily spread raw sausages. The 14 isolates belonged to 6 different O-serotypes. 4 VT1-, 8 VT2- and 2 VT1/VT2-producers were found. 4 isolates belonged to serogroups which were already described in WHO tables and associated with EHEC infections in human beings. One strain of serogroup O22: H8, isolated from a "Teewurst", possessed the complete virulence gene combination of EHEC (eae, hlyA, stx). The detection procedure, already successfully used for detection and isolation of VTEC from raw milk, soft cheese and raw minced beef showed a sensitivity of approximately 10 CfU/25 g of raw sausages. It has to be considered that VTEC are frequently (8.8%) present in shortly ripened raw sausages. The group of easily spread raw sausages has a higher VTEC-contamination rate than firm raw sausages. Raw sausages, especially easy to spread types, belong to the risk foods for EHEC-infections in human beings.
authors.affiliation: Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin (BgVV), Fachgebiet Bakteriologie Dessau.
authors.forename: M
authors.initials: M
authors.lastname: Timm
authors.forename: H
authors.initials: H
authors.lastname: Klie
authors.forename: H
authors.initials: H
authors.lastname: Richter
authors.forename: P
authors.initials: P
authors.lastname: Gallien
authors.forename: K W
authors.initials: KW
authors.lastname: Perlberg
authors.forename: S
authors.initials: S
authors.lastname: Lehmann
authors.forename: D
authors.initials: D
authors.lastname: Protz
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D003296:Cooking; D003603:Cytotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000635:transmission* / Q000662:veterinary; D006801:Humans; D008461:Meat Products / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D012703:Serotyping; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D022622:Shiga Toxin 1
vernacular_title: Untersuchungen zum Nachweis und Vorkommen von Verotoxin-bildenden Escherichia coli (VTEC) in Rohwurst.