Systemic anti-IFN-gamma treatment and role of macrophage subsets in the foreign body reaction to dermal sheep collagen in rats.
The application of a biomaterial induces a foreign body reaction. By controlling this reaction, biocompatibility could be improved. We previously demonstrated that impregnation of a biodegradable biomaterial with antibodies against interferon-gamma (IFN-gamma) inhibits the foreign body reaction. In this study we investigate whether systemic administration of the antibody can induce similar reactions. Several parameters are compared between control and anti-IFN-gamma-treated rats: cellular ingrowth; degradation of the biomaterial; ingrowth of macrophage (MO) subsets, T cells, B cells, NK cells, and granulocytes; and expression of the major histocompatibility complex class II (MHC class II) molecule on antigen presenting cells. Treatment with anti-IFN-gamma results in increased cellular ingrowth and biomaterial degradation and a decreased expression of MHC class II. Overall, systemic treatment with anti-IFN-gamma is insufficient to modulate the foreign body reaction. This suggests an alternative mechanism for MO activation besides IFN-gamma. The role of T cells and MO subsets in the foreign body reaction is discussed.
Khouw
I M
IM
Cell Biology and Biomaterials, University of Groningen, Bloemsingel 10, 9712 KZ Groningen, The Netherlands. I.KhouwWmed.rug.nl
van Wachem
P B
PB
van der Worp
R J
RJ
van den Berg
T K
TK
de Leij
L F
LF
van Luyn
M J
MJ
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000633:toxicity*; D003094:Collagen / Q000276:immunology / Q000633:toxicity*; D005549:Foreign-Body Reaction / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors*; D008262:Macrophage Activation; D008264:Macrophages / Q000145:classification / Q000276:immunology* / Q000473:pathology; D008297:Male; D051381:Rats; D012756:Sheep; D012867:Skin / Q000276:immunology
D000906:Antibodies; D001672:Biocompatible Materials; D007371:Interferon-gamma; D003094:Collagen