title: Genome-wide transcriptional analysis of aerobic and anaerobic chemostat cultures of Saccharomyces cerevisiae.
abstract: The yeast Saccharomyces cerevisiae is unique among eukaryotes in exhibiting fast growth in both the presence and the complete absence of oxygen. Genome-wide transcriptional adaptation to aerobiosis and anaerobiosis was studied in assays using DNA microarrays. This technique was combined with chemostat cultivation, which allows controlled variation of a single growth parameter under defined conditions and at a fixed specific growth rate. Of the 6,171 open reading frames investigated, 5,738 (93%) yielded detectable transcript levels under either aerobic or anaerobic conditions; 140 genes showed a >3-fold-higher transcription level under anaerobic conditions. Under aerobic conditions, transcript levels of 219 genes were >3-fold higher than under anaerobic conditions.
authors.affiliation: Institute of Molecular Plant Sciences, Leiden University, 2333 AL Leiden, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: ter Linde
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: R W
authors.initials: RW
authors.lastname: Davis
authors.forename: H Y
authors.initials: HY
authors.lastname: Steensma
authors.forename: J P
authors.initials: JP
authors.lastname: van Dijken
authors.forename: J T
authors.initials: JT
authors.lastname: Pronk
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D016680:Genome, Bacterial; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D005947:Glucose