Human rhinovirus HRV14 uncoats from early endosomes in the presence of bafilomycin.
Determination of infectious progeny virus and in vivo labelling with [(35)S]methionine followed by immunoprecipitation demonstrates that the major receptor group human rhinovirus HRV14 is able to infect HeLa cells in the presence of the V-ATPase inhibitor bafilomycin A1. However, host cell shut off is delayed and viral yield is decreased in the presence of the drug. Uncoating can thus take place under conditions that prevent endosomal acidification indicating that it is catalysed by the viral receptor alone. Since transport is arrested in early endosomes upon inhibition of vesicle acidification, the data also suggest that productive uncoating takes place from early endocytic compartments.
Bayer
N
N
Department of General and Experimental Pathology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria.
Prchla
E
E
Schwab
M
M
Blaas
D
D
Fuchs
R
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002213:Capsid / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D011992:Endosomes / Q000821:virology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018942:Macrolides; D011233:Precipitin Tests; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D012367:RNA, Viral / Q000378:metabolism*; D012229:Rhinovirus / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D018942:Macrolides; D012367:RNA, Viral; C040929:bafilomycin A1; D006180:Proton-Translocating ATPases