title: Identification of serotypes of Rickettsia tsutsugamushi by nested polymerase chain reaction.
abstract: Nested polymerase chain reaction (PCR) was applied to identify the serotypes of Rickettsia tsutsugamushi isolated from patients. The primers used for PCR were based on the nucleotide sequences encoding a 56 kDa antigen of rickettsiae. Comparing to the conventional immunofluorescence assay (IFA), which displays a considerable degree of cross-reactivity among different species, the result obtained suggests that the polymerase chain reaction method is much more reliable than IFA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Shieh
authors.forename: H L
authors.initials: HL
authors.lastname: Chen
authors.forename: H Y
authors.initials: HY
authors.lastname: Chen
authors.forename: G R
authors.initials: GR
authors.lastname: Wang
authors.forename: C B
authors.initials: CB
authors.lastname: Horng
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D012285:Orientia tsutsugamushi / Q000145:classification*; D016133:Polymerase Chain Reaction; D012703:Serotyping