Differential effector and secretory functions of microglial cell lines derived from BCG-resistant and -susceptible congenic mouse strains.
Using congenic strains of mice susceptible (bcg(s)) or resistant (bcg(r)) to BCG, murine microglial cell lines, RR4.R (BCG-resistant) and RR8.S (BCG-susceptible), were established in vitro. Comparative studies revealed that, although phagocytic to a similar extent, RR4.R cells were more active than RR8.S cells in terms of antimycobacterial activity. Interestingly, cells of resistant genotype secreted more nitric oxide, TNF-alpha and IL-12, but less IL-6, than susceptible cells, when stimulated with IFN-gamma alone or in combination with lipopolysaccharide. Nevertheless, no significant differences were observed between the two cell lines in terms of IL-1 beta or IL-10 secretion, or on assessment of cytokine production following exposure to a massive dose of lipopolysaccharide. Overall, these data provide the first evidence that resistant/susceptible genotype influences antimycobacterial activity, NO and cytokine production in microglial cells, the prototype of cerebral macrophages.
Puliti
M
M
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Mazzolla
R
R
Brozzetti
A
A
Neglia
R
R
Radzioch
D
D
Bistoni
F
F
Blasi
E
E
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D027682:Cation Transport Proteins; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000494:pharmacology; D018664:Interleukin-12 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000502:physiology*; D009163:Mycobacterium bovis / Q000276:immunology*; D009569:Nitric Oxide / Q000096:biosynthesis; D000099173:Natural Resistance-associated Macrophage Protein 1
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D016207:Cytokines; D007371:Interferon-gamma; D018664:Interleukin-12; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D009569:Nitric Oxide; D000099173:Natural Resistance-associated Macrophage Protein 1