title: Cloning of fish enzymes and other fish protein genes.
abstract: Fish metabolism needs special enzymes that have maximum activity at very different conditions than their mammalian counterparts. Due to the differences in activity, these enzymes, especially cold-adapted proteases, could be used advantageously for the production of some foods. In addition to the enzymes, this review describes some other unique fish polypeptides such as antifreeze proteins, fluorescent proteins, antitumor peptides, antibiotics, and hormones, that have already been cloned and used in food processing, genetic engineering, medicine, and aquaculture. Recombinant DNA technology, which allows these biological molecules to be cloned and overexpressed in microorganisms is also described, highlighting innovative applications. The expected impact of cloning fish proteins in different fields of technology is discussed.
authors.affiliation: Department of Food Science, McGill University, Quebec, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Macouzet
authors.forename: B K
authors.initials: BK
authors.lastname: Simpson
authors.forename: B H
authors.initials: BH
authors.lastname: Lee
mesh_terms: D000818:Animals; D021301:Antifreeze Proteins; D002403:Cathepsins / Q000235:genetics; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004798:Enzymes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005399:Fishes / Q000378:metabolism*; D005511:Food Handling / Q000379:methods*; D005615:Freezing; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods; D006023:Glycoproteins / Q000235:genetics*; D006728:Hormones / Q000235:genetics*; D011480:Protease Inhibitors / Q000737:chemistry; D011503:Transglutaminases / Q000235:genetics; D014357:Trypsin / Q000235:genetics
chemical_list: D021301:Antifreeze Proteins; D004798:Enzymes; D006023:Glycoproteins; D006728:Hormones; D011480:Protease Inhibitors; D003577:Cytochrome P-450 Enzyme System; D011503:Transglutaminases; D002403:Cathepsins; D014357:Trypsin