High-throughput nonradioisotopic determination of binding of platelet-derived growth factor to platelet-derived growth factor receptor beta-extracellular domain using biotinylated ligand with enzyme-linked immunosorbent assay.
Mahoney
C W
CW
Discovery Research Laboratory, Tanabe Pharmaceutical Co., Toda, Saitama, 335, Japan.
D000077214:Becaplermin; D001665:Binding Sites; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011485:Protein Binding; D020574:Proto-Oncogene Proteins c-sis; D017479:Receptors, Platelet-Derived Growth Factor / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D001710:Biotin; D017479:Receptors, Platelet-Derived Growth Factor