title: Discordance between fetal RhD typing using molecular methods and neonatal typing with serology.
abstract: Polymerase chain reaction (PCR)-based genotyping on amniotic fluid in an RhD-negative alloimmunized woman predicted an RhD-negative fetal blood type. The neonate was RhD-positive and developed hemolytic disease. Discrepant results were also observed on paternal testing. PCR analysis with a different set of primers correctly predicted the RhD-positive fetal and paternal blood type. Use of more than one set of primers and parental testing can avoid some of the problems associated with use of PCR genotyping.
authors.affiliation: Department of Obstetrics and Gynecology, Divisions of Maternal Fetal Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: I B
authors.initials: IB
authors.lastname: Van Den Veyver
authors.forename: J
authors.initials: J
authors.lastname: Yankowitz
authors.forename: S B
authors.initials: SB
authors.lastname: Subramanian
authors.forename: K F
authors.initials: KF
authors.lastname: Dorman
authors.forename: K J
authors.initials: KJ
authors.lastname: Moise
mesh_terms: D000328:Adult; D000653:Amniotic Fluid; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004899:Erythroblastosis, Fetal / Q000628:therapy; D005188:False Negative Reactions; D005260:Female; D005838:Genotype; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012203:Rh Isoimmunization; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System