title: Lysophosphatidic acid-induced Ca(2+) mobilization in the neural retina of chick embryo.
abstract: Lysophosphatidic acid (LPA) plays various roles in the regulation of cell growth as a lipid mediator. We studied the effect of LPA on intracellular Ca(2+) concentration ([Ca2+]i) with Fura-2 in the neural retina of chick embryo during neurogenesis. Bath application of LPA (1-100 microM) to the embryonic day 3 (E3) chick retina caused an increase in [Ca2+](i) in a dose-dependent manner, with an EC(50) value of 9.2 microM. The Ca(2+) rise was also evoked in a Ca(2+)-free medium, suggesting that release of Ca(2+) from intracellular Ca(2+) stores (Ca(2+) mobilization) was induced by LPA. U-73122, a blocker of phospholipase C (PLC), inhibited the Ca(2+) rise to LPA. Pertussis toxin partially inhibited the Ca(2+) rise to LPA, indicating that G(i)/G(o) protein was at least partially involved in the LPA response. The developmental profile of the LPA response was studied from E3 to E13. The Ca(2+) rise to LPA declined drastically from E3 to E7, in parallel with decrease in mitotic activity of retinal progenitor cells. The signal transduction pathway and developmental profile of the Ca(2+) response to LPA were the same as those of the Ca(2+) response to adenosine triphosphate (ATP), which enhances the proliferation of retinal progenitor cells. The coapplication of LPA with ATP resulted in enhancement of Ca(2+) rise in the E3 chick retina. Our results show that LPA induces Ca(2+) mobilization in the embryonic chick retina during neurogenesis.
authors.affiliation: Department of Physiology, Osaka University Medical School, Yamadaoka 2-2, Suita 565-0871, Japan.
authors.forename: W L
authors.initials: WL
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Sugioka
authors.forename: M
authors.initials: M
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D007700:Kinetics; D008246:Lysophospholipids / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D012160:Retina / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008246:Lysophospholipids; C049925:fura-2-am; D002118:Calcium; D016257:Fura-2