Visualisation of tissue kallikrein and kinin receptors in oesophageal carcinoma.
Gene expression of tissue kallikrein (TK) and the subsequent formation of kinins may stimulate proliferation of tumour cells by increasing vascular permeability, and enhancing metastasis. Our study was undertaken to immunolocalise TK and B2 receptor in specimens of oesophageal carcinoma immediately following oesophagectomy. The diagnosis and grading of oesophageal carcinoma was performed histologically. For localisation of TK, tissue prokallikrein (T proK) and the kinin receptors, slide-mounted tissue sections were subjected to peroxidase-antiperoxidase method and immunofluorescent staining using primary antibodies raised against each antigen. TK was immunolocalised in giant cells of both well and poorly differentiated squamous cell carcinomas (SCCs). The precursor of the enzyme, T proK, was also immunolocalised. The most intense tissue-prokallikrein labelling was observed in giant tumour cells. Kinin receptors were immunolocalised in giant tumour cells, and the mature squamous cells. These findings suggests that TK, tissue-prokallikrein and kinin receptors are present in oesophageal carcinoma, and may be important in the process of tumourogenesis in this disease.
Dlamini
Z
Z
Department of Clinical and Experimental Pharmacology, University of Natal Medical School, Durban, South Africa.
Raidoo
D
D
Bhoola
K
K
D004938:Esophageal Neoplasms / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D007610:Kallikreins / Q000032:analysis*; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000032:analysis*
D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D007610:Kallikreins