A nonisotopic estrogen receptor-based assay to detect estrogenic compounds.
We have used the ligand binding domain of the recombinant human estrogen receptor (hER) to develop a nonisotopic assay for detection of estrogenic compounds. The assay is based on competition of the estrogenic ligand with 17beta-estradiol for binding to the receptor, which leaves 17beta-estradiol free to bind to an anti-17beta-estradiol antibody. Unbound anti-17beta-estradiol antibody then binds to immobilized 17beta-estradiol-protein conjugate (to which hER is unable to bind for steric reasons), and is detected by an enzyme-labeled anti-rabbit IgG antibody. We used the assay to detect estrogenic compounds (mainly members of the flavonoid group of plant polyphenols) in a variety of commonly consumed plant foods.
Garrett
S D
SD
Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, United Kingdom.
Lee
H A
HA
Morgan
M R
MR
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004967:Estrogens / Q000032:analysis* / Q000378:metabolism; D005504:Food Analysis; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D004967:Estrogens; D008024:Ligands; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins