title: Effects of oxidative damage of membrane protein thiol groups on erythrocyte membrane viscoelasticities.
abstract: In three oxidative damaging systems: the diamide-mercaptoethanol redox modification system (DM), the pyrogallol oxygen free radicals system (PG) and the hypoxanthine-xanthine oxidase oxygen free radical system (HXO), the effect of erythrocyte membrane oxidative damage on membrane viscoelasticities was investigated with micropipette aspiration method. The experimental results indicated that erythrocyte membrane oxidative damage has a great influence upon the membrane mechanical properties. The oxidative damage led to decrease of contents of membrane protein thiol radical. The scanning of SDS-PAGE presented that membrane proteins form the higher molecular weight component (HMP) by the cross-linking of membrane protein thiol radicals that might hinder the conformational change of membrane protein. This might be the reason for the increased membrane elastic modulus and viscous coefficient upon treating erythrocytes with the oxidative damaging systems. A significant negative logarithm regression relation was found between the membrane elastic modulus, mu, or viscoefficient, eta, and the contents of membrane protein thiol radicals. These experimental results suggested that thiol radicals oxidative damage reaction due to the superoxides anions (*O2-) may be an important molecular mechanism inducing changes of membrane viscoelasticities or whole cell deformability of erythrocyte under physiological and pathological oxidative stress.
authors.affiliation: College of Bioengineering, Open Lab for Biomechanics and Biorheology under the Ministry of Education, Chongqing University, PR. of China. bio@cqu.edu.cn
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: G
authors.initials: G
authors.lastname: Song
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Long
authors.forename: S
authors.initials: S
authors.lastname: Cai
mesh_terms: D001809:Blood Viscosity / Q000187:drug effects*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004548:Elasticity / Q000187:drug effects; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D005609:Free Radicals; D006801:Humans; D019271:Hypoxanthine / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects; D008565:Membrane Proteins / Q000737:chemistry / Q000187:drug effects*; D008623:Mercaptoethanol / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011748:Pyrogallol / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds / Q000097:blood*; D013481:Superoxides / Q000494:pharmacology; D014783:Viscosity / Q000187:drug effects; D014969:Xanthine Oxidase / Q000494:pharmacology
chemical_list: D005609:Free Radicals; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D011748:Pyrogallol; D013481:Superoxides; D019271:Hypoxanthine; D008623:Mercaptoethanol; D004228:Dithionitrobenzoic Acid; D014969:Xanthine Oxidase