Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion.
Following appropriate antigen-specific stimulation, CD4(+) and CD8(+) T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell surface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4(+) and CD8(+) T lymphocytes. The power of this technique is demonstrated here for HLA-A0201-restricted influenza matrix protein peptide 58-66-specific CD8(+) cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.
Brosterhus
H
H
Miltenyi Biotec GmbH Bergisch Gladbach, Germany.
Brings
S
S
Leyendeckers
H
H
Manz
R A
RA
Miltenyi
S
S
Radbruch
A
A
Assenmacher
M
M
Schmitz
J
J
D017951:Antigen Presentation; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D016207:Cytokines / Q000276:immunology*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*
D000941:Antigens; D016207:Cytokines; D011948:Receptors, Antigen, T-Cell