title: Ontogeny of puromycin-sensitive and insensitive aminopeptidase activities in several subcellular fractions of the rat brain.
abstract: Puromycin-sensitive and insensitive aminopeptidase (aminopeptidase M) activities are measured in several subcellular fractions of the rat brain cortex and subcortex during the first postnatal month. Tyr-beta-naphthylamide has been used as substrate and 20 microM puromycin as selective inhibitor. We have found that puromycin-sensitive aminopeptidase activity increases twofold in the synaptosomal and mitochondrial fractions in the first 6-9 postnatal days, just during the period of axonal and dendritic growth. This enzyme also has significant age-related changes in the nuclear fraction. The developmental pattern is different, depending on the subcellular fraction analyzed. Significant developmental changes of puromycin-insensitive aminopeptidase (aminopeptidase M) are only found in the myelinic and microsomal fractions and they are less significant than those found in the puromycin-sensitive aminopeptidase. It has been suggested that these enzyme activities could be involved in processes of cell proliferation, differentiation, and maturation.
authors.affiliation: Department of Physiology, Medical School, University of the Basque Country, Bilbao, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: de Gandarias
authors.forename: J
authors.initials: J
authors.lastname: Irazusta
authors.forename: J
authors.initials: J
authors.lastname: Gil
authors.forename: D
authors.initials: D
authors.lastname: Fernández
authors.forename: A
authors.initials: A
authors.lastname: Varona
authors.forename: L
authors.initials: L
authors.lastname: Casis
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018826:CD13 Antigens / Q000187:drug effects*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000201:enzymology*
chemical_list: D011691:Puromycin; D018826:CD13 Antigens