title: An improved method to distinguish Entamoeba histolytica and Entamoeba dispar.
abstract: A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. The amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. The method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.
authors.affiliation: Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. magomes@mono.icb.ufmg.br
authors.forename: M A
authors.initials: MA
authors.lastname: Gomes
authors.forename: J B
authors.initials: JB
authors.lastname: Pesquero
authors.forename: C
authors.initials: C
authors.lastname: Furst
authors.forename: P R
authors.initials: PR
authors.lastname: Valle
authors.forename: J L
authors.initials: JL
authors.lastname: Pesquero
authors.forename: E F
authors.initials: EF
authors.lastname: Silva
mesh_terms: D000818:Animals; D004747:Entamoeba / Q000145:classification* / Q000235:genetics; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA