Ecdysone has an effect on the regeneration of midgut epithelial cells that is distinct from 20-hydroxyecdysone in the silkworm Bombyx mori.
We investigated the effects of two ecdysteroids, ecdysone (E) and 20-hydroxyecdysone (20E), on silkworm larval development. Silkworm larvae, Bombyx mori, were fed an artificial diet supplemented with 20E during the fourth instar to promote premature molting. At the onset of the fifth instar, these precocious fifth-instar larvae were fed diets supplemented with either E or 20E to determine the effects of the two ecdysteroids on the morphology of midgut epithelial cells. Regeneration of midgut epithelial cells normally occurs only during the molting period. However, in larvae fed E, complete replacement of midgut epithelial cells was observed 24 h before the larvae entered apolysis. In larvae fed 20E, the morphology of midgut epithelial cells was disrupted, leading to death of the larvae during the fifth instar. We also observed similar differences in the effects of the two ecdysteroids in an in vitro experiment. These results suggest that E has a specific effect on the morphological change of midgut epithelial cells in precocious fifth-instar larvae that is distinct from 20E.
Tanaka
Y
Y
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, 305-8634, Japan. yoshiaki@nises.affrc.go.jp
Yukuhiro
F
F
D000818:Animals; D012831:Bombyx / Q000254:growth & development*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D004032:Diet; D004440:Ecdysone / Q000378:metabolism / Q000494:pharmacology*; D004441:Ecdysterone / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000648:ultrastructure; D007422:Intestines / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008247:Lysosomes / Q000187:drug effects; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects
D004440:Ecdysone; D004441:Ecdysterone