A therapeutic vaccine approach to stimulate axon regeneration in the adult mammalian spinal cord.
Axon growth inhibitors associated with myelin play an important role in the failure of axon regeneration in the adult mammalian central nervous system (CNS). Several inhibitors are present in the mature CNS. We now present a novel therapeutic vaccine approach in which the animals' own immune system is stimulated to produce polyclonal antibodies that block myelin-associated inhibitors without producing any detrimental cellular inflammatory responses. Adult mice immunized in this manner showed extensive regeneration of large numbers of axons of the corticospinal tracts after dorsal hemisection of the spinal cord. The anatomical regeneration led to recovery of certain hind limb motor functions. Furthermore, antisera from immunized mice were able to block myelin-derived inhibitors and promote neurite growth on myelin in vitro.
Huang
D W
DW
Centre for Research in Neuroscience, The Montreal General Hospital Research Institute and McGill University, Quebec, Canada.
McKerracher
L
L
Braun
P E
PE
David
S
S
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001369:Axons / Q000502:physiology*; D002417:Cattle; D005260:Female; D007114:Immunization; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D009186:Myelin Sheath / Q000276:immunology; D009412:Nerve Fibers / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D011712:Pyramidal Tracts / Q000503:physiopathology; D013116:Spinal Cord / Q000276:immunology / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000276:immunology / Q000503:physiopathology*; D014612:Vaccines / Q000627:therapeutic use*
D000906:Antibodies; D014612:Vaccines