Impact of a preceding striatal excitotoxic lesion and treatment with ciliary neurotrophic factor on striatal graft survival.
The survival of grafted embryonic striatal tissue, dissected from the lateral ganglionic eminence, depends on the status of the host striatum. We found significantly larger volumes of surviving graft tissue and of striatal-like tissue (P-zone) within the graft, when the host striatum had been subjected to an excitotoxic lesion prior to transplantation surgery. Concomitantly the numbers of surviving grafted cells, assessed in both cresyl violet-stained sections and in sections stained with an immunohistochemical marker for striatal neurons, increased as compared to when graft tissue was placed in an intact unlesioned striatum. Finally, we examined the impact of treatment of the donor tissue with ciliary neurotrophic factor (CNTF) on graft survival. CNTF has previously been shown to protect striatal neurons against excitotoxic insults both in vitro and in vivo, but it did not improve striatal graft survival when added to the cell suspension prior to implantation.
Petersén
A
A
Wallenberg Neuroscience Center, Department of Physiological Sciences, Lund University, Sweden. asa.petersen@mphy.lu.se
Emgård
M
M
Brundin
P
P
D000818:Animals; D002452:Cell Count; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D003342:Corpus Striatum / Q000502:physiology* / Q000601:surgery / Q000637:transplantation; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D020934:Ciliary Neurotrophic Factor; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins