[Sensitivity of soil bacteria isolated from the alienated zone around the Chernobyl Nuclear Power Plant to various stress factors].
Seventy strains of chemoorganotrophic bacteria isolated by our group in 1993-1994 from soil sampled in the zone around the Chernobyl Nuclear Power Plant (ChNPP) were studied with respect to their sensitivity to various stress factors damaging DNA. Bacillus subtilis, B. cereus (both spores and vegetative cells), Methylobacterium extorquens, M. mesophilicum, and unidentified pigmented bacteria were found to be the most resistant to ultraviolet (UV) radiation, exhibiting LD90 values of 40 to more than 211 J/m2. The same bacteria, as well as Bacillus polymyxa, were tolerant to hydrogen peroxide (lethal concentrations of H2O2 ranged from 0.3 to 1.0 M); i.e., UV-resistant strains were also tolerant to hydrogen peroxide and vice versa. Fluorescent pseudomonads were the most sensitive to both UV radiation and H2O2, showing LD90 from 6 to 18 J/m2 and a lethal concentration of H2O2 lower than 0.1 M. All of the soil samples collected in the alienated zone around the ChNPP, where the radioactivity of the soil had decreased from 1000 to 2 microCi/kg soil over the period from 1987 to 1995, contained not only resistant bacteria but also a small number of bacteria sensitive to UV radiation and H2O2.
Romanovskaia
V A
VA
Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Rokitko
P V
PV
Malashenko
Iu R
IuR
Krishtab
T P
TP
Chernaia
N A
NA
D001419:Bacteria / Q000235:genetics*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004269:DNA, Bacterial / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000633:toxicity; D016877:Oxidants / Q000633:toxicity; D011210:Power Plants; D011836:Radiation Tolerance; D018788:Radioactive Hazard Release; D012988:Soil Microbiology; D012990:Soil Pollutants, Radioactive; D014455:Ukraine; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D016877:Oxidants; D012990:Soil Pollutants, Radioactive; D006861:Hydrogen Peroxide
Chuvstvitel'nost' k stressovym faktoram pochvennykh bakteriĭ, izolirovannykh iz zony otchuzhdeniia Chernobyl'skoĭ AES.