title: Alpha- and beta-carotenes in low density lipoprotein are the preferred target for nitric oxide-induced oxidation.
abstract: Whereas low plasma levels of carotenes are strongly associated with the elevated risk of atherosclerosis, the reason for this is still unknown. We hypothesized that lipoprotein oxidation in the arterial wall might selectively deplete carotenes, thus explaining the observed effects. In order to assess this hypothesis, we incubated plasma low density lipoprotein (LDL) with different oxidants and measured the consumption of carotenes and tocopherols. We found that when LDL oxidation was induced by nitric oxide, both alpha- and beta-carotene were consumed at a significantly higher relative rate than alpha- or gamma-tocopherol. In contrast, superoxide, peroxynitrite, hypochlorite or transition metal ions were unable to induce selective consumption of carotenes in LDL. These data suggest that the decreased plasma levels of alpha- and beta-carotene frequently measured in atherosclerosis may be related to their preferred consumption by reactive nitrogen species in the arterial wall.
authors.affiliation: Biochemisches Labor, Pav. 39, Medizinische Kern- und Poliklinik, Universitätskrankenhaus Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany. kontush@uke.uni-hamburg.de
authors.forename: A
authors.initials: A
authors.lastname: Kontush
authors.forename: W
authors.initials: W
authors.lastname: Weber
authors.forename: U
authors.initials: U
authors.lastname: Beisiegel
mesh_terms: D002338:Carotenoids / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D014810:Vitamin E / Q000378:metabolism; D019207:beta Carotene / Q000378:metabolism*
chemical_list: D008077:Lipoproteins, LDL; D019207:beta Carotene; D014810:Vitamin E; D009569:Nitric Oxide; D002338:Carotenoids; C041635:alpha-carotene