A DNA fragment of Leptospira interrogans encodes a protein which shares epitopes with equine cornea.
Horses infected with Leptospira interrogans present several clinical disorders, one of them being recurrent uveitis. An antigenic relationship between this bacterium and equine cornea has been described in previous studies. With the aim to make progress on defining the molecular basis and pathogenesis of equine recurrent uveitis, here we describe the cloning of one DNA fragment from a Leptospira interrogans serovar pomona genomic lambda gt11 library. Although there are references of transcription of leptospiral genes in E. coli from their own leptospiral promoters, in this recombinant construction the leptospiral DNA was located under the control of lacZ promoter since no expression could be detected in the absence of IPTG. This clone, isolated by expression screening with polyclonal serum raised against equine corneal proteins, encodes a 90 kDa protein of L. interrogans which crossreacts with equine cornea as proved Western-blotting. Antibodies directed against this leptospiral protein strongly recognised a 66 kDa equine corneal protein, one of those recognised by an anti-equine cornea serum. Our findings suggest that an immune response to 90 kDa protein participates in pathogenesis of equine uveitis.
Lucchesi
P M
PM
Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Argentina. paulaluc@vet.unicen.edu.ar
Parma
A E
AE
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D003315:Cornea / Q000276:immunology*; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000235:genetics*; D000939:Epitopes; D006734:Horse Diseases / Q000209:etiology*; D006736:Horses; D007921:Leptospira interrogans / Q000235:genetics / Q000276:immunology*; D014605:Uveitis / Q000209:etiology / Q000662:veterinary*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000939:Epitopes