Effect of dextran derivatives on arginine amidase activities released from isolated rabbit arteries.
In the present research we examined the levels and types of arginine amidase activities that were released from isolated rabbit arteries treated with heparin or chondroitin sulfate. Heparin accelerated the release of arginine amidase activity from the isolated rabbit ear artery, the induction was not significant; a slight increase in activity was observed in the level of arginine amidase released from isolated rabbit aorta, but no significant difference was observed. On the other hand, it was revealed that the addition of chondroitin sulfate, accelerated this release from isolated rabbit ear artery with 5% significant differences. After the addition of chondroitin sulfate, the arginine amidase activity released from isolated rabbit arteries was analyzed using various affinity adsorption methods. This analysis confirmed the presence of two types of fibrinolytic enzymes: plasminogen/plasmin activity and plasminogen activators, but no thrombin was detected.
Takeuchi
K
K
Department of Clinical Pharmacology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
Miyazaki
K
K
Ishii-Nozawa
R
R
Katayama
M
M
Matsuda
Y
Y
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000187:drug effects / Q000201:enzymology; D001120:Arginine / Q000378:metabolism; D001158:Arteries / Q000187:drug effects / Q000201:enzymology*; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D008239:Lysine / Q000378:metabolism; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism*
D000925:Anticoagulants; D003911:Dextrans; D006493:Heparin; D002809:Chondroitin Sulfates; D001120:Arginine; D012697:Serine Endopeptidases; C402942:arginine amidase; D008239:Lysine