Oxalate-induced and cell-cycle-dependent expression of nuclear pore complex oxalate binding protein gp210.
The effect of oxalate, a constituent of renal stone, on the expression of nuclear pore complex oxalate binding protein (gp210) in Vero monkey kidney cells was examined. The expression of this protein was found to increase more in mitotic phase than in S phase, suggesting cell cycle dependency. Exposure of cells to oxalate-containing growth medium resulted in a relative increase in nuclear pore complex oxalate binding protein in each stage of cell cycle. The concentration of this protein was found to increase six times in the telophase stage of the cells exposed to high concentrations of oxalate in the growth medium, though slight reduction in cell density was observed. Structural analogues of oxalate did not show any stimulatory effect on expression of this oxalate binding protein. Hence, the expression of the nuclear pore complex oxalate binding protein gp210 was specific to oxalate and is cell cycle dependent.
Vijaya
R
R
Department of Medical Biochemistry, Dr. Alm Post Graduate Institute of Basic Medical Sciences, Chennai, 600113, India.
Kannapiran
M
M
Selvam
R
R
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002453:Cell Cycle / Q000502:physiology*; D002522:Chlorocebus aethiops; D019343:Citric Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D008293:Malates / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010070:Oxalates / Q000494:pharmacology*; D010072:Oxamic Acid / Q000494:pharmacology; D019802:Succinic Acid / Q000494:pharmacology; D014709:Vero Cells
D008293:Malates; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; D010070:Oxalates; D019343:Citric Acid; C030298:malic acid; D019802:Succinic Acid; D010072:Oxamic Acid