Regulated expression of green fluorescent protein under the control of Aureobasidium pullulans xylanase gene xynA.
A mutant form of the jellyfish cDNA encoding green fluorescent protein (GFP) was fused to the promoter of the Aureobasidium pullulans xylanase gene xynA and the expression vector pxynEGFP was introduced into A. pullulans. In a manner consistent with regulation of the native xynA gene, gfp activity was induced by xylose and repressed by glucose. The marker may be useful for monitoring populations of A. pullulans in situ and for identifying transcriptional control elements of xynA.
Vanden Wymelenberg
A
A
USDA, Forest Service, Forest Products Laboratory, Madison, WI 53705, USA.
Cullen
D
D
Spear
R
R
Andrews
J
J
D000818:Animals; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases; D005434:Flow Cytometry; D005658:Fungi / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005822:Genetic Vectors; D005947:Glucose / Q000494:pharmacology; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D014994:Xylose / Q000494:pharmacology; D014995:Xylosidases / Q000235:genetics*
D003470:Culture Media; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D014994:Xylose; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases; D005947:Glucose