title: Cross-Canada spread of methicillin-resistant Staphylococcus aureus via transplant organs.
abstract: We report our investigation of the transmission of methicillin-resistant Staphylococcus aureus (MRSA) through transplantation. The kidneys, liver, and corneas were harvested from a child who died in Nova Scotia. Several days postmortem it was learned that culture of a premortem endotracheal tube aspirate from the donor yielded MRSA. Both kidneys were transplanted into a child in Nova Scotia and the liver into a child in Alberta. Both recipients subsequently became blood culture-positive for MRSA. One corneal ring from the donor was MRSA-positive. All four MRSA isolates were mecA-positive by polymerase chain reaction (PCR). The relatedness of the MRSA isolates was examined by restriction fragment length polymorphism (RFLP) analysis, a 16S-23S ribosomal PCR typing method, and comparison of antibiograms. Results were identical for all four MRSA isolates. These findings indicate that MRSA from the donor was transferred to recipients during implantation of harvested organs in Alberta and Nova Scotia, a cross-Canada spread.
authors.affiliation: Queen Elizabeth II Health Sciences Centre, and Department of Medicine, Dalhousie University, Halifax, Nova Scotia. ljohnsto@is.dal.ca
authors.forename: L
authors.initials: L
authors.lastname: Johnston
authors.forename: L
authors.initials: L
authors.lastname: Chui
authors.forename: N
authors.initials: N
authors.lastname: Chang
authors.forename: S
authors.initials: S
authors.lastname: Macdonald
authors.forename: M
authors.initials: M
authors.lastname: McKenzie
authors.forename: W
authors.initials: W
authors.lastname: Kennedy
authors.forename: D
authors.initials: D
authors.lastname: Haldane
authors.forename: R
authors.initials: R
authors.lastname: Bethune
authors.forename: G
authors.initials: G
authors.lastname: Taylor
authors.forename: M
authors.initials: M
authors.lastname: Hanakowski
authors.forename: G
authors.initials: G
authors.lastname: Tyrrell
mesh_terms: D000293:Adolescent; D002170:Canada; D005260:Female; D006801:Humans; D007223:Infant; D016030:Kidney Transplantation / Q000009:adverse effects*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000635:transmission*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D014019:Tissue Donors