Vaccination of chickens with a temperature-sensitive mutant of Salmonella enteritidis.
One-day old chickens were inoculated with temperature-sensitive mutant E/1/3 of S. enteritidis. Two routes of inoculation were used: oral and intraperitoneal (ip). One group of chickens were given two oral inoculations (oral-oral). A second group received two ip inoculations (ip-ip). A third group received the first dose orally and the second ip (oral-ip) and the fourth group was given the first dose ip and the second dose orally (ip-oral). The vaccine strain was safe even when inoculated at high doses, and induced strong protection against virulent S. enteritidis strain after oral challenge. Results show that vaccination with mutant E/1/3 reduced the number of animals shedding the pathogen after challenge. Furthermore, animals immunized oral-oral and oral-ip showed a significant reduction in cecal and spleen colonization by virulent Salmonella.
Cerquetti
M C
MC
Centro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Departamento de Microbiología Aplicada, Serrano 669, 1414, Buenos Aires, Argentina. cerquetti@cotelcam.com.ar
Gherardi
M M
MM
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D008297:Male; D009154:Mutation; D012477:Salmonella enteritidis / Q000276:immunology*; D013696:Temperature; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated