Cloning, sequencing, and characterization of genomic subtracted sequences from Listeria monocytogenes.
Individual sequences of a genomic subtracted, PCR-amplified, mixed-sequence probe (GS probe) were cloned and sequenced. The GS probe differentiated restriction fragment length polymorphism patterns for Listeria monocytogenes but did not hybridize with members of other bacterial genera. Sequence analysis identified several L. monocytogenes sequences already present in the GenBank database; the putative identities of other sequences were inferred from homology data, and still other sequences did not exhibit significant levels of homology with any GenBank sequences.
Wu
F M
FM
Department of Food Science, Purdue University, West Lafayette, Indiana 47907, USA.
Muriana
P M
PM
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D015342:DNA Probes; D016208:Databases, Factual; D016680:Genome, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015342:DNA Probes