title: Prevalent nosocomial gram negative aerobic bacilli and their antimicrobial susceptibility pattern in intensive care unit.
abstract: OBJECTIVE
  To determine the type of prevalent aerobic gram-negative bacilli and their sensitivity pattern among nosocomial isolates.
  
  
  METHODS
  Prospective collection of clinically significant nosocomial gram negative bacilli.
  
  
  METHODS
  Tertiary care hospital in Karachi.
  
  
  METHODS
  One hundred isolates were identified by standard methods and minimum inhibitory concentration was checked by epsilometer test.
  
  
  RESULTS
  The most frequent isolates were Eschericia coli (43%) followed by Klebsiella pneumoniae (18%) Acinetobacter spp. (7%) Enterobacter spp. (7%) and Klebsiella spp. other than pneumoniae (7%). Most of the isolates of dominant species (E. coli and Klebsiella pneumoniae) were multiresistant including third generation cephalosporins.
  
  
  CONCLUSIONS
  This study indicates that most effective antibiotics imipenem and amikacin inhibited most of the isolates. Imipenem alone or amikacin in combination with one broad spectrum beta-lactam drug should be used in initial empiric therapy in any life threatening nosocomial infection.
authors.affiliation: Department of Clinical Microbiology, Dr. Ziauddin Hospital, Karachi.
authors.forename: A
authors.initials: A
authors.lastname: Zafar
mesh_terms: D000583:Amikacin / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003428:Cross Infection / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy*; D006801:Humans; D015378:Imipenem / Q000627:therapeutic use; D007231:Infant, Newborn; D007362:Intensive Care Units; D008826:Microbial Sensitivity Tests; D011446:Prospective Studies; D013845:Thienamycins / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D013845:Thienamycins; D015378:Imipenem; D000583:Amikacin