title: Improved recovery of Mycobacterium tuberculosis from pleural aspirates: bedside inoculation, heparinized containers and liquid culture media.
abstract: The effects of bedside inoculation, heparinized containers and liquid culture media on the recovery of Mycobacterium tuberculosis from pleural aspirates were evaluated in this study. Of 155 patients, 63 were diagnosed to have pleural effusion tuberculous in origin. The overall recovery of M. tuberculosis was 57.1%. Bedside inoculation of the specimens produced a significantly higher yield than laboratory inoculation using non-heparinized specimens. When the pleural aspirates were transported in heparinized containers, the recovery rate was comparable to that from bedside inoculation, but lower when non-heparinized containers were used. No significant difference was found in recovery rate between the two liquid media, but the rate was significantly higher with the use of liquid media than conventional solid media. Thus, bedside inoculation of pleural aspirates, use of heparinized containers for transport for delayed inoculation in the laboratory and use of liquid culture media are recommended.
authors.affiliation: Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong.
authors.forename: A F
authors.initials: AF
authors.lastname: Cheng
authors.forename: V H
authors.initials: VH
authors.lastname: Tai
authors.forename: M S
authors.initials: MS
authors.lastname: Li
authors.forename: C H
authors.initials: CH
authors.lastname: Chan
authors.forename: C F
authors.initials: CF
authors.lastname: Wong
authors.forename: W W
authors.initials: WW
authors.lastname: Yew
authors.forename: M
authors.initials: M
authors.lastname: Hui
authors.forename: C Y
authors.initials: CY
authors.lastname: Chan
authors.forename: J C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000925:Anticoagulants; D001431:Bacteriological Techniques; D003470:Culture Media; D006493:Heparin; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D014396:Tuberculosis, Pleural / Q000382:microbiology*
chemical_list: D000925:Anticoagulants; D003470:Culture Media; D006493:Heparin