Novel streptomycin and spectinomycin resistance gene as a gene cassette within a class 1 integron isolated from Escherichia coli.
The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance gene dfr7 in a class 1 integron. The integron was located on a plasmid and was identified in a pathogenic porcine Escherichia coli isolate.
Sandvang
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Danish Veterinary Laboratory, DK-1790 Copenhagen V, and Department of Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark. ds@svs.dk
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics; D010957:Plasmids; D016133:Polymerase Chain Reaction; D000198:Spectinomycin / Q000494:pharmacology*; D013307:Streptomycin / Q000494:pharmacology*; D013552:Swine; D014296:Trimethoprim Resistance / Q000235:genetics
D000900:Anti-Bacterial Agents; D000198:Spectinomycin; D009713:Nucleotidyltransferases; C022678:streptomycin 3''-adenylyltransferase; D013307:Streptomycin