title: Hepatic zonation of the formation and hydrolysis of cholesteryl esters in periportal and perivenous parenchymal cells.
abstract: The periportal (PP) and perivenous (PV) zones of the liver acinus differ in enzyme complements and capacities for cholesterol and bile acid synthesis and other metabolic processes. The aim of this investigation was to determine the acinar distribution of the catalytic activity of the enzymes governing the formation and hydrolysis of cholesteryl esters using PP and PV hepatocytes from normal or cholestyramine-fed rats. The hepatocyte subpopulations were isolated by centrifugal elutriation, characterized according to the distribution pattern of a number of cell parameters and marker enzymes, and assayed for acyl-CoA:cholesterol acyltransferase (ACAT) and lysosomal, cytosolic and microsomal cholesteryl ester hydrolase (CEH). In normally fed rats, no zonation was found in the activity of lysosomal CEH and ACAT, and the activity of both cytosolic and microsomal CEH zonated toward the PV zone of the acinus. Concentrations of free and esterified cholesterol in homogenates, cytosol, and microsomes of PP and PV cells were, however, similar. Cholestyramine raised significantly the PV/PP ratio of ACAT because of an exclusive PP reduction of activity and abolished the heterogeneity in microsomal CEH because of a greater inhibitory PV response, whereas the PV dominance of cytosolic CEH and the homogeneous distribution of lysosomal CEH were unaffected. These results demonstrated homogeneity within the liver acinus for the enzymatic degradation of endocyted lipoprotein-derived cholesteryl esters, a structural zonation of the cytosolic CEH and a dynamic zonation of ACAT and the microsomal CEH, with a PV dominance of the enzymatic capacity for the degradation of stored cholesteryl esters in normal livers.
authors.affiliation: Department of Physiology, University of the Basque Country Medical School, Bilbao, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: Romero
authors.forename: O
authors.initials: O
authors.lastname: Fresnedo
authors.forename: E
authors.initials: E
authors.lastname: Isusi
authors.forename: J
authors.initials: J
authors.lastname: Barrionuevo
authors.forename: B
authors.initials: B
authors.lastname: Ochoa
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D002788:Cholesterol Esters / Q000378:metabolism*; D002792:Cholestyramine Resin / Q000008:administration & dosage; D003600:Cytosol / Q000201:enzymology; D004951:Esterification; D005260:Female; D006503:Hepatic Veins; D006868:Hydrolysis; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D002787:Sterol Esterase / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D002788:Cholesterol Esters; D002792:Cholestyramine Resin; D002785:Sterol O-Acyltransferase; D002787:Sterol Esterase