Confirmation of the detection of HLA-A*0104N in a family: a PCR-SSP reaction for the allelic detection of HLA-A*0104N.
The allele A*0104N has been detected in a family with a patient requiring a bone marrow transplant. The allele was found as a consequence of a discrepant result when family members were typed using serology and polymerase chain reaction using sequence-specific primers (PCR-SSP). Serological typing gave an apparent HLA-A 'blank' while PCR-SSP revealed the presence of an A*01 allele in three family members who were serologically negative for A1. Sequencing-based typing (SBT) was then used to establish that the allele was A*0104N. A PCR-SSP reaction was subsequently designed and used for the allelic detection of A*0104N. The study highlights the potential risks involved if molecular technology is used for typing, unless all non-expressed alleles are specifically detected.
Moses
J H
JH
Tissue Typing Services, Australian Red Cross Blood Service, Sydney, NSW 2000, Australia.
Downes
J
J
McClenahan
W
W
Clow
N
N
Kennedy
C
C
Greville
W D
WD
Dunckley
H
H
D000483:Alleles; D016026:Bone Marrow Transplantation / Q000276:immunology; D017931:DNA Primers; D005260:Female; D005805:Genes, MHC Class I; D015788:HLA-A1 Antigen / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015788:HLA-A1 Antigen