The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes.
The metabolism of ethidium bromide by isolated rat hepatocytes is significantly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-methylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- and 1.5-fold increase, respectively in the amount of the principal metabolite, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced by pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidium glucuronide and a transient unidentified species, have been detected by HPLC and are formed only by hepatocytes from animals pre-treated with 3-MC.
Tettey
J N
JN
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
Skellern
G G
GG
Midgley
J M
JM
Grant
M H
MH
Pitt
A R
AR
D000818:Animals; D001711:Biotransformation; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D004996:Ethidium / Q000493:pharmacokinetics*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008748:Methylcholanthrene / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014344:Trypanocidal Agents / Q000493:pharmacokinetics*
D014344:Trypanocidal Agents; D008748:Methylcholanthrene; D004996:Ethidium; D010634:Phenobarbital