title: Antigen-specific, IgE-selective unresponsiveness induced by antigen-liposome conjugates. Comparison of four different conjugation methods for the coupling of antigen to liposome.
abstract: BACKGROUND
  We have previously reported that ovalbumin (OVA) coupled with liposome via glutaraldehyde (GA) induced OVA-specific- and IgE-selective unresponsiveness in mice.
  
  
  METHODS
  In this study, OVA-liposome conjugates were made using four different coupling protocols: via GA, N-(6-maleimidocaproyloxy) succinimide (EMCS), disuccinimidyl suberate (DSS) and N-succimidyl-3(2-pyridyldithio)propionate (SPDP) and the induction of antigen-specific IgG and IgE antibody production was investigated for each. In addition, antigen-specific cytokine production by spleen cells of mice immunized either with OVA-liposome or with OVA adsorbed with aluminum hydroxide was investigated.
  
  
  RESULTS
  OVA-liposome conjugates coupled via GA or DSS did not induce anti-OVA IgE antibody production but induced substantial anti-OVA IgG antibody production. On the other hand, the induction of anti-OVA IgE unresponsiveness by OVA-liposome conjugates coupled via EMCS or SPDP was incomplete. The amount of interleukin 4 (IL-4) produced by spleen cells stimulated in vitro with OVA correlated well with anti-OVA IgE antibody production in donor mice. However, the production of no other cytokine, i.e., IL-2, IL-5, IL-10 or interferon-gamma, was correlated with in vivo IgE antibody production.
  
  
  CONCLUSIONS
  OVA-liposome coupled via GA or DSS induced complete suppression of anti-OVA IgE production. The results in this study further suggest that the regulation of IgE antibody production does not necessarily correlate with so-called Th1 cytokine production.
authors.affiliation: NOF Corp., Tsukuba Research Laboratory, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Nishinohara
authors.forename: Y
authors.initials: Y
authors.lastname: Takita
authors.forename: S
authors.initials: S
authors.lastname: Naito
authors.forename: A
authors.initials: A
authors.lastname: Horino
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Taneichi
authors.forename: Y
authors.initials: Y
authors.lastname: Ami
authors.forename: Y
authors.initials: Y
authors.lastname: Suzaki
authors.forename: K
authors.initials: K
authors.lastname: Komuro
authors.forename: T
authors.initials: T
authors.lastname: Uchida
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D005976:Glutaral / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology* / Q000378:metabolism; D007158:Immunologic Techniques; D008081:Liposomes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D013154:Spleen / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism; D013997:Time Factors; D014612:Vaccines
chemical_list: D000941:Antigens; D016207:Cytokines; D008081:Liposomes; D013388:Succinimides; D014612:Vaccines; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; D007073:Immunoglobulin E; C072682:N-(epsilon-maleimidocaproyloxy)succinimide; D010047:Ovalbumin; D005976:Glutaral; C019358:disuccinimidyl suberate