title: Three-dimensional visualization of the Golgi apparatus: observation of Brunner's gland cells by a confocal laser scanning microscope.
abstract: The three-dimensional structure of the Golgi apparatus in cells of the Brunner's gland in the mouse was observed by using a confocal laser scanning microscope. Two lectins, FITC-labeled soybean agglutinin and Texas red-labeled Griffonia simplicifolia agglutinin II, were used to visualize the whole Golgi apparatus. Staining with the former lectin, which has been known to label the cis-stacks, showed a lacy dome-like structure situated in the supranuclear region. Staining with the latter lectin, known to label the intermediate-to-trans-stacks and the secretory granules, showed a dome-like structure consisting of network and cobblestone-like patterns in the same region and also granular stainings near the surface of the cobblestone-like patterns and the apical region of a cell. Double-staining demonstrated that the soybean agglutinin-labeled network always surrounded the G. simplicifolia agglutinin II-stained structure. Based on these observations, we propose a new three-dimensional model of the Golgi apparatus: it forms a dome-like structure over a nucleus, a network of cis-stacks forms its outer boundary, and this outer boundary is lined and paved with successive intermediate and trans-stacks. It is thought that secretory granules are released toward the internal space of the Golgi apparatus and transported to the apical cytoplasm through the holes of the network.
authors.affiliation: Department of Anatomy, Hiroshima University School of Medicine, Hiroshima, 734-8551, Japan. etchan@mcai.med.hiroshima-u.ac.jp
authors.forename: E
authors.initials: E
authors.lastname: Suzaki
authors.forename: K
authors.initials: K
authors.lastname: Kataoka
mesh_terms: D000373:Agglutinins; D000818:Animals; D002011:Brunner Glands / Q000648:ultrastructure*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006056:Golgi Apparatus / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D014966:Xanthenes
chemical_list: D000373:Agglutinins; D005456:Fluorescent Dyes; D014966:Xanthenes; C034657:Texas red; D016650:Fluorescein-5-isothiocyanate