title: L-selectin expression on thymic emigrants defines two distinct tissue-migration pathways.
abstract: We have studied the appearance and phenotype of recent thymic emigrants in blood, spleen and lymph nodes (LN) of neonatal lambs. Using in situ labelling of thymocytes with fluoroscein isothiocyanate (FITC), we examined the expression of the LN homing receptor L-selectin on alphabeta and gammadelta subsets of recent thymic emigrants 24 hr after labelling. There were marked differences in the proportions of CD4+, CD8+ and gammadelta T-cell receptor (TCR+) cells exported from the thymus to spleen compared to lymph nodes. Spleen was enriched in CD8+ and gammadelta TCR+ emigrants while LN were enriched in CD4+ emigrants. There were also marked differences in the expression of L-selectin by emigrants homing to spleen compared with those homing to lymph nodes. While the majority of thymic emigrants in LN expressed L-selectin, considerably fewer emigrants in spleen were L-selectin+. The presence of large numbers of CD8+ L-selectin- and gammadelta TCR+ L-selectin- thymic emigrants homing to spleen raises the possibility that unique homing receptor specificities underpin the migration of T cells to spleen as distinct from lymph nodes.
authors.affiliation: Laboratory for Foetal and Neonatal Immunology, The University of Melborne, Parkville, Victoria, Australia.
authors.forename: J E
authors.initials: JE
authors.lastname: Holder
authors.forename: W G
authors.initials: WG
authors.lastname: Kimpton
authors.forename: E A
authors.initials: EA
authors.lastname: Washington
authors.forename: R N
authors.initials: RN
authors.lastname: Cahill
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D005434:Flow Cytometry; D017404:In Situ Hybridization, Fluorescence; D019041:L-Selectin / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D009928:Organ Specificity / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis; D012756:Sheep; D013154:Spleen / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta; D019041:L-Selectin