title: The effects of sodium deoxycholate, lactulose and glutamine on bacterial translocation in common bile duct ligated rats.
abstract: OBJECTIVE
  Sepsis is a major cause of post-operative morbidity and mortality in obstructive jaundice as a result of bacterial translocation from the gut. This study was conducted to investigate the effects of glutamine, lactulose, and the bile salt Na deoxycholate in preventing bacterial translocation in an animal model where obstructive jaundice was developed by common bile duct ligation.
  
  
  METHODS
  Fifty Wistar albino rats were divided into 5 groups of 10 animals each. The animals in groups I-IV underwent common bile duct ligation and received, respectively, either saline, Na deoxycholate, lactulose or glutamine, orally. Group V had sham ligation and received saline orally. The animals were sacrificed at the end of the 7th day, and serum concentrations of bilirubin, aspartate aminotransferase (ALT), alanine aminotransferase (ALT), and alkaline phosphatase (AP) were measured. In addition, mesenteric lymph nodes were removed and cultured together with cecal content. Histopathologic examination of terminal ileum specimens was made.
  
  
  RESULTS
  Na deoxycholate, lactulose and glutamine all reduced bacterial translocation rates to mesenteric lymph nodes (p<0.05), with glutamine causing the greatest effect. Na deoxycholate and lactulose prevented bacterial translocation by causing a decrease in cecal intraluminal bacterial content (p<0.001), while glutamine exerted its effect by preserving intestinal mucosal integrity.
  
  
  CONCLUSIONS
  The integrity of the intestinal mucosal barrier is of paramount importance in preventing bacterial translocation, and the measures taken to protect mucosal integrity reduce bacterial translocation to a greater extent than those taken to decrease the number of bacteria in the gut.
authors.affiliation: Department of Surgery, Institute of Oncology, Istanbul, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Erbil
authors.forename: E
authors.initials: E
authors.lastname: Berber
authors.forename: S
authors.initials: S
authors.lastname: Ozarmagan
authors.forename: R
authors.initials: R
authors.lastname: Seven
authors.forename: L
authors.initials: L
authors.lastname: Eminoglu
authors.forename: A
authors.initials: A
authors.lastname: Calis
authors.forename: V
authors.initials: V
authors.lastname: Olgaç
authors.forename: N
authors.initials: N
authors.lastname: Gürler
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D001651:Cholestasis, Extrahepatic / Q000382:microbiology* / Q000473:pathology; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004195:Disease Models, Animal; D005973:Glutamine / Q000494:pharmacology*; D007082:Ileum / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D007792:Lactulose / Q000494:pharmacology*; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003840:Deoxycholic Acid; D005973:Glutamine; D007792:Lactulose