Mitochondrial function in cell wall glycoprotein synthesis in Saccharomyces cerevisiae NCYC 625 (Wild type) and [rho(0)] mutants.
We studied phosphopeptidomannans (PPMs) of two Saccharomyces cerevisiae NCYC 625 strains (S. diastaticus): a wild type strain grown aerobically, anaerobically, and in the presence of antimycin and a [rho(0)] mutant grown aerobically and anaerobically. The aerobic wild-type cultures were highly flocculent, but all others were weakly flocculent. Ligands implicated in flocculation of mutants or antimycin-treated cells were not aggregated as much by concanavalin A as were those of the wild type. The [rho(0)] mutants and antimycin-treated cells differ from the wild type in PPM composition and invertase, acid phosphatase, and glucoamylase activities. PPMs extracted from different cells differ in the protein but not in the glycosidic moiety. The PPMs were less stable in mitochondrion-deficient cells than in wild-type cells grown aerobically, and this difference may be attributable to defective mitochondrial function during cell wall synthesis. The reduced flocculation of cells grown in the presence of antimycin, under anaerobiosis, or carrying a [rho(0)] mutation may be the consequence of alterations of PPM structures which are the ligands of lectins, both involved in this cell-cell recognition phenomenon. These respiratory chain alterations also affect peripheral, biologically active glycoproteins such as extracellular enzymes and peripheral PPMs.
Iung
A R
AR
Faculté de Pharmacie-UMR UHP-CNRS 7564-LCPE Biochimie Microbienne, Université Henri Poincaré, Nancy 1, 54001 Nancy Cedex, France.
Coulon
J
J
Kiss
F
F
Ekome
J N
JN
Vallner
J
J
Bonaly
R
R
D000332:Aerobiosis; D000693:Anaerobiosis; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D008351:Mannans / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism*; D010748:Phosphopeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D014462:Ultrafiltration; D020741:rho GTP-Binding Proteins / Q000235:genetics*
D008351:Mannans; D008562:Membrane Glycoproteins; D010748:Phosphopeptides; C072108:phosphopeptidomannan; D003208:Concanavalin A; C032456:antimycin; D000968:Antimycin A; D020741:rho GTP-Binding Proteins