Ultrastructural properties of laser-irradiated and heat-treated dentin.
Previous studies using scanning electron microscopy and infrared absorption spectroscopy reported that laser irradiation causes compositional changes in enamel. The purpose of this study was to evaluate the ultrastructural and compositional changes in dentin caused by irradiation with a short-pulse laser (Q-switched Nd:YAG). The irradiated and non-irradiated areas of the lased dentin samples were investigated by scanning (SEM) and transmission electron microscopy (TEM), micro-micro electron diffraction, and electron microprobe analysis of dispersive energy (EDX). Heat-treated dentin was similarly investigated. This study demonstrated that laser irradiation resulted in the recrystallization of dentin apatite and in the formation of additional calcium phosphate phases consisting of magnesium-substituted beta-tricalcium phosphate, beta-TCMP, beta-(Ca,Mg)3(PO4)2, and tetracalcium phosphate, TetCP, Ca4(PO4)O. TEM analyses of the modified and unmodified zones of the irradiated areas showed two types of crystal populations: much larger crystals from the modified zone and crystals with size and morphology similar to those of dentin apatite in the unmodified zone. The morphology of crystals in the modified zones in the irradiated dentin resembled those of dentin sintered at 800 or 950 degrees C. In the irradiated areas (modified and unmodified zones), the Ca/P ratio was lower compared with that in the non-irradiated dentin. The Mg/Ca ratio in the modified zones was higher than that in the unmodified zones and in the non-irradiated dentin. In sintered dentin, the Mg/Ca ratio increased as a function of sintering temperature. The ultrastructural and compositional changes observed in laser-irradiated dentin may be attributed to high temperature and high pressure induced by microplasma during laser irradiation. These changes may alter the solubility of the irradiated dentin, making it less susceptible to acid dissolution or to the caries process.
Rohanizadeh
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R
Laboratoire de recherche sur les tissus calcifiés et les biomatériaux, Faculté de Chirurgie Dentaire, Nantes, France.
LeGeros
R Z
RZ
Fan
D
D
Jean
A
A
Daculsi
G
G
D002118:Calcium / Q000032:analysis; D003460:Crystallization; D003804:Dentin / Q000737:chemistry / Q000528:radiation effects* / Q000648:ultrastructure; D003806:Dentin Permeability; D017886:Durapatite / Q000737:chemistry; D004577:Electron Probe Microanalysis; D006801:Humans; D007834:Lasers; D008274:Magnesium / Q000032:analysis; D017348:Microscopy, Electron, Scanning Transmission; D009354:Neodymium; D010710:Phosphates / Q000032:analysis
D010710:Phosphates; D009354:Neodymium; D017886:Durapatite; D008274:Magnesium; D002118:Calcium