title: Preparation of chitooligosaccharides from chitosan by a complex enzyme.
abstract: Chitosan of 24% degree of acetylation was depolymerized by a mixture of cellulase, alpha amylase, and proteinase to give the title oligosaccharides. The removal of products by membrane separation permitted yield maximization of products having degree of polymerization in the 3-10 range.
authors.affiliation: Department of Biochemical Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Science, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Du
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Mitsutomi
authors.forename: S
authors.initials: S
authors.lastname: Aiba
mesh_terms: D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan; D004798:Enzymes / Q000378:metabolism; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000096:biosynthesis*
chemical_list: D004798:Enzymes; D009844:Oligosaccharides; D002686:Chitin; D048271:Chitosan