Non-suppressed conductivity and indirect UV detection of carboxylic acids in environmental samples by ion-exclusion chromatography using 2,6-pyridinedicarboxylic acidic eluent.
2,6-Pyridinedicarboxylic acid (PDCA) was evaluated as an eluent for indirect UV and non-suppressed conductivity detection of carboxylic acids in ion-exclusion chromatography. The effect of PDCA concentration on the separation and detection sensitivity was investigated. The reasonable resolutions between carboxylic acids were achieved using 1 mM PDCA eluent. Detection limits were 1.0-7.0 microM for conductivity detection and 8-30 microM for UV detection. Compared to the eluent containing 1 mM sulfuric acid, the method offers a high resolution and high detection sensitivity for both detectors due to its high molar absorptivity and low background conductance. The proposed method was demonstrated to be useful for the determination of carboxylic acids in environmental samples with direct sample injection.
Chen
Z
Z
Centre for Legumes in Mediterranean Agriculture/Soil Science and Plant Nutrition, The University of Western Australia, Nedlands, Australia. zuliang@adl.clw.csiro.au
Tang
C
C
Xu
J
J
D002264:Carboxylic Acids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D007202:Indicators and Reagents; D010848:Picolinic Acids; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013464:Sulfuric Acids
D002264:Carboxylic Acids; D004785:Environmental Pollutants; D007202:Indicators and Reagents; D010848:Picolinic Acids; D013464:Sulfuric Acids; C033158:sulfuric acid; C004860:dipicolinic acid