Effect of calcium soaps of fatty acids and administration of somatotropin on milk production, preovulatory follicular development, and plasma and follicular fluid lipid composition in high yielding dairy cows.
The effect of fat and bovine somatotropin (bST) on preovulatory follicular hormones and lipids was evaluated by feeding cows for 150 d from parturition a control diet, a control diet plus 0.55 kg/d of calcium soaps of fatty acids, or a control diet with 500 mg of bST injected every 14 d. Fourteen days after a synchronized or natural estrus, cows were injected with a PGF2 alpha analogue; 48 h later, follicular fluid from all ovarian follicles > 8 mm was aspirated. Cows fed fat or injected with bST produced more milk and milk solids than did control cows, and cows on the bST treatment lost more body condition after calving than did cows on the other treatments. Both treatments changed the proportion of estradiol-active follicles (> 400 ng of estradiol/ml of follicular fluid) and the correlation between follicular fluid estradiol concentration and the total number large follicles per cow. In follicles aspirated between 60 and 90 DIM the percentage of estradiol-active follicles was 67, 40, and 0 for cows on the control, calcium soaps of fatty acids, and bST treatments, respectively. After 90 DIM, no differences existed between treatments in the percentage of estradiol-active follicles. Estradiol concentration in follicular fluid was correlated with DIM at follicle aspiration (r = 0.51). The proportion of oleic acid in free fatty acids in plasma at 50 DIM was lower in control cows and was lower in follicular fluid of estradiol-active follicles. Both calcium soaps of fatty acids and bST had a considerable effect on follicular development and activity and the composition of fatty acids in follicles.
Moallem
U
U
Institute of Animal Science, Volcani Center, Bet Dagan, Israel.
Folman
Y
Y
Bor
A
A
Arav
A
A
Sklan
D
D
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D004958:Estradiol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D007774:Lactation / Q000502:physiology; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D006080:Ovarian Follicle / Q000502:physiology*; D010060:Ovulation; D011374:Progesterone / Q000378:metabolism; D012915:Soaps
D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D012915:Soaps; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D013006:Growth Hormone; D002118:Calcium