Different V beta usage in antigen-specific and alloreactive T cells specific for the same MHC elements.
V beta 8 has been shown to be used in the majority of antigen specific T cell hybridomas restricted by I-Ad and I-Ed. The usage of V beta 8 in these T cell responses in vivo was confirmed as V beta 8 depleted BALB/c mice responded weakly to these I-Ad- and I-Ed-restricted antigens. We used this deletion assay to further examine if V beta 8 is similarly dominantly used in alloreactive T cell specific for I-Ad/Ed. The depletion of V beta 8-population in allogenic mice did not affect the alloreactive responses toward I-Ad/Ed. Although specific for the same MHC, there is no apparent overlap on the use of TCR V beta 8 between alloreactive T cells and antigen-specific T cells.
Fan
M D
MD
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC.
Liang
H E
HE
Lai
M Z
MZ
D000595:Amino Acid Sequence; D000818:Animals; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; C024138:I-E-antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta