title: Aminopyrazine analogues as chemiluminescence derivatization reagents for pyruvic acid.
abstract: Aminopyrazine analogues were studied as sensitive and selective chemiluminescence derivatization reagents for pyruvic acid. These analogues reacted with pyruvic acid under acidic conditions at 100 degrees C to produce Cypridina luciferin derivatives, which exhibit chemiluminescence by reaction with hydrogen peroxide in the presence of potassium t-butoxide in dimethylformamide. Of the four aminopyrazine analogues (2-amino-5-phenylpyrazine, 2-amino-5-(4-hydroxyphenyl)pyrazine, 2-amino-5-(3,4, 5-trimethoxyphenyl)pyrazine, and 2-aminoquinoxaline), in the present test 2-amino-5-(3,4,5-trimethoxyphenyl) pyrazine was the most sensitive for pyruvic acid, and the chemiluminescence intensity was about four times higher than that obtained with aminopyrazine.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakata
authors.forename: N
authors.initials: N
authors.lastname: Sakata
authors.forename: Y
authors.initials: Y
authors.lastname: Ohba
authors.forename: K
authors.initials: K
authors.lastname: Zaitsu
mesh_terms: D007202:Indicators and Reagents; D008163:Luminescent Measurements; D011719:Pyrazines / Q000737:chemistry*; D019289:Pyruvic Acid / Q000032:analysis*; D011810:Quinoxalines / Q000737:chemistry*
chemical_list: C403605:2-amino-5-(3,4,5-trimethoxyphenyl)pyrazine; C403607:2-amino-5-phenylpyrazine; C403606:2-aminoquinoxaline; D007202:Indicators and Reagents; D011719:Pyrazines; D011810:Quinoxalines; D019289:Pyruvic Acid