title: Examination of ras oncoproteins in human plasma from healthy controls and workers exposed to petroleum emissions, including benzene-related compounds.
abstract: Ras oncoproteins in blood plasma from workers exposed to petroleum emissions and unexposed controls were examined from Polish and Estonian samples. Twenty-four workers and 35 unexposed controls were examined from Poland and 97 exposed and 40 unexposed controls from Estonia. Of the Estonian workers, 50 were exposed to benzene in a benzene production plant and 47 to polyaromatic hydrocarbons and benzene in a cokery. Blood plasma proteins were separated by gel electrophoresis, transferred to a nitrocellulose membrane by Western blotting and detected by chemiluminescence using a monoclonal antibody as the primary antibody. There were no statistically significant differences between the exposed and the control groups in either the Polish or the Estonian samples.
authors.affiliation: BIBRA International, Carshalton, Surrey, UK. danderson@bibra.co.uk
authors.forename: D
authors.initials: D
authors.lastname: Anderson
authors.forename: J A
authors.initials: JA
authors.lastname: Hughes
authors.forename: M H
authors.initials: MH
authors.lastname: Brinkworth
authors.forename: A
authors.initials: A
authors.lastname: Cebulska-Wasilewska
authors.forename: E
authors.initials: E
authors.lastname: Nizankowska
authors.forename: B
authors.initials: B
authors.lastname: Graca
authors.forename: T
authors.initials: T
authors.lastname: Veidebaum
authors.forename: K
authors.initials: K
authors.lastname: Peltonen
authors.forename: M
authors.initials: M
authors.lastname: Sorsa
mesh_terms: D000395:Air Pollutants, Occupational / Q000097:blood*; D001554:Benzene / Q000032:analysis*; D001555:Benzene Derivatives / Q000032:analysis*; D015415:Biomarkers / Q000097:blood*; D015153:Blotting, Western; D002617:Chemical Industry; D004591:Electrophoresis, Polyacrylamide Gel; D004784:Environmental Monitoring / Q000379:methods; D004957:Estonia; D006801:Humans; D016273:Occupational Exposure / Q000032:analysis*; D010578:Petroleum; D011044:Poland; D016283:Proto-Oncogene Proteins p21(ras) / Q000097:blood*; D012621:Seasons
chemical_list: D000395:Air Pollutants, Occupational; D001555:Benzene Derivatives; D015415:Biomarkers; D010578:Petroleum; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D001554:Benzene