title: The disruption of macaque CD4+ T-cell repertoires during the early simian immunodeficiency virus infection.
abstract: T-cell receptor (TCR) complementarily determining region 3 (CDR3) spetratyping analysis was employed to assess the ability of an AIDS virus to disrupt CD4 + T-cell repertoires during the primary infection. Rhesus and pig-tailed macaques infected with simian immunodeficiency virus (SIV)mac 251 and SIVsmmFGb, respectively, were evaluated. Following SIV infection, the macaques exhibited an apparent decline of CD4 + peripheral blood lymphocyte (PBL) counts, which was associated with a change in CDR3 profiles from multiple-length distribution to one- or two-length dominance in the selected TCR Vbeta-expressing CD4 + PBL subpopulations. Molecular analysis of the perturbed cell subpopulations suggested that the CD4 + T cells bearing the dominant CDR3 length were clonally expanded. These results indicate that SIV infection can induce a disruption of macaque CD4 + T-cell repertoires during the primary infection. The finding in this study, therefore, suggests that the virus-induced clonal dominance can contribute to the disruption of CD4 + T-cell repertoires.
authors.affiliation: Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: D
authors.initials: D
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Kou
authors.forename: C
authors.initials: C
authors.lastname: Ibegbu
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.forename: D
authors.initials: D
authors.lastname: Lee-Parritz
authors.forename: L
authors.initials: L
authors.lastname: Shen
authors.forename: P K
authors.initials: PK
authors.lastname: Sehgal
authors.forename: H M
authors.initials: HM
authors.lastname: McClure
authors.forename: P
authors.initials: P
authors.lastname: Morrison
authors.forename: C
authors.initials: C
authors.lastname: Bogle
authors.forename: N
authors.initials: N
authors.lastname: Sehgal
authors.forename: A J
authors.initials: AJ
authors.lastname: Nahmias
authors.forename: Z W
authors.initials: ZW
authors.lastname: Chen
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D018791:CD4 Lymphocyte Count; D002999:Clone Cells; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008254:Macaca nemestrina; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000503:physiopathology; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000472:pathogenicity; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D015704:CD4 Antigens