Tracking components of the transcription apparatus in living cells.
Transcription regulatory proteins are an integral component of the cell nucleus and a great deal of work has been done to characterize the subnuclear distribution of these proteins. Much of the early work on this subject was done with immunofluorescence. The development of the green fluorescent protein (GFP) as a marker for intracellular protein localization has allowed for the real time study of protein localization and dynamics in living cells. In this review, an overview of the way in which GFP can be utilized to study protein localization is presented.
Baumann
C T
CT
Laboratory of Receptor Biology and Gene Expression, NCI/NIH, Building 41, Room B602, 41 Library Drive, MSC 5055, Bethesda, Maryland, 20892-5055, USA.
Reyes
J C
JC
D002468:Cell Physiological Phenomena; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D014158:Transcription, Genetic / Q000502:physiology*
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins