Vitellins and vitellogenins of Dysdercus koenigii (Heteroptera: Pyrrhocoridae)--identification, purification and temporal pattern.
Two vitellins, VtA and VtB, were purified from the eggs of Dysdercus koenigii by gel filtration and ion exchange chromatography. VtA and VtB have molecular weights of 290 and 260 kDa, respectively. Both Vts are glycolipoproteinaceous in nature. VtA is composed of three polypeptides of M(r) 116, 92 and 62 kDa while VtB contained an additional subunit of M(r) 40 kDa. All subunits except the 116-kDa subunit are glycolipopolypeptides. Polyclonal antibody raised against VtA (anti-VtA antibody) cross-reacted with VtB and also with vitellogenic haemolymph and ovaries and pre-vitellogenic fat bodies, but not with haemolymph from either adult male, fifth instar female, or pre-vitellogenic females demonstrating sex and stage specificity of the Vts. Immunoblots in the presence of anti-VtA revealed two proteins (of 290 and 260 kDa) in both vitellogenic haemolymph and pre-vitellogenic fat bodies that are recognised as D. koenigii Vgs. In newly emerged females, Vgs appeared on day 1 in fat bodies and on day 3 in haemolymph and ovaries. Vg concentration was maximum on day 2 in fat body, day 4 in haemolymph and day 7 in ovary. Although the biochemical and temporal characteristics of these proteins show similarity to some hemipterans, they are strikingly dissimilar with those of a very closely related species.
Venugopal
K J
KJ
Department of Zoology, Banaras Hindu University, Varanasi, India. venu@ces.iisc.ernet.in
Kumar
D
D
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D020062:Heteroptera / Q000502:physiology*; D007814:Larva; D008297:Male; D008970:Molecular Weight; D009865:Oocytes / Q000737:chemistry / Q000502:physiology; D009928:Organ Specificity; D014819:Vitellogenins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D004527:Egg Proteins; D014819:Vitellogenins