Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis.
A monoclonal antibody (MAb)-based latex agglutination (MAb-LA) test was developed to rapidly identify Burkholderia pseudomallei in hemoculture of patients with septicemic melioidosis. The method was evaluated in a clinical situation on 396 hemocultures positive for bacterial growth, of which 75 cultures were positive for B. pseudomallei by conventional biochemical tests. The sensitivity and specificity of the MAb-LA test were 95% and 100%, respectively. The positive and negative predictive values were 100% and 99%. The method is highly reliable and suitable for rapid diagnosis of septicemic melioidosis, reducing the time normally required from a minimum of 3-4 days by conventional methods to less than 30 hr. Most of these 30 hr are involved in growing up enough bacteria to perform the MAb-LA test, which itself takes only 1 min.
Samosornsuk
N
N
Khon Kaen Hospital, Faculty of Associated Medical Sciences, Khon Kaen University, Thailand.
Lulitanond
A
A
Saenla
N
N
Anuntagool
N
N
Wongratanacheewin
S
S
Sirisinha
S
S
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000097:blood; D016470:Bacteremia / Q000175:diagnosis*; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007841:Latex Fixation Tests / Q000592:standards*; D008554:Melioidosis / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial