Identification of new genes ndr2 and ndr3 which are related to Ndr1/RTP/Drg1 but show distinct tissue specificity and response to N-myc.
Ndr1 was isolated as a gene upregulated in N-myc mutant mouse embryos and is repressed by N-myc and c-myc. Consistent with Myc regulation, the same gene was also isolated as one sensitive to transformation (Drg1), and in addition as one induced under a few stress conditions (RTP). Two new genes, Ndr2 and Ndr3, were identified which encode proteins highly related to Ndr1/RTP/Drg1 and constitute the Ndr gene family. Ndr2 and Ndr3 are under spatio-temporal regulations distinct from Ndr1, and are not activated in N-myc mutants. When whole embryo RNA was analyzed, Ndr3 expression was already high at 9.5 days postcoitus (dpc), while expression of Ndr2 and Ndr1 became significant after 12.5 dpc and 13. 5 dpc, respectively. At 14.5 dpc, expression of these genes partially overlaps, but many tissues are unique to one of them. For instance, Ndr1 is strongly expressed in the liver and gut epithelium, Ndr2 in the ventricular zone throughout the CNS, and Ndr3 in the spinal cord and the thymus rudiment. Genes of the Ndr family probably have tissue-dependent allotments of the possibly related functions.
Okuda
T
T
Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Kondoh
H
H
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism; D020224:Expressed Sequence Tags; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D018878:Limb Buds / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D055456:Nodal Signaling Ligands; D009928:Organ Specificity; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D047908:Intracellular Signaling Peptides and Proteins; C485172:Ndr2 protein, mouse; C113775:Ndr2 protein, vertebrate; C402397:Ndrg3 protein, mouse; D055456:Nodal Signaling Ligands; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta