Ca2+ mobilization and activation of extracellular acidification by carbachol in acutely dispersed cells from guinea pig detrusor: Fura 2 fluorometry and microphysiometry using the cytosensor.
The study aim was to develop a simple in vitro model for pharmacophysiological investigation of urinary bladder smooth muscles. Smooth muscle cells from guinea pig detrusor were dissociated, and the suspended cells were stimulated with carbachol (CCh), an acetylcholine receptor agonist. Cytosolic Ca2+ levels were determined using Fura 2 fluorescence and extracellular acidification rates were monitored by the Cytosensor microphysiometer. CCh dose-dependently increased cytosolic Ca2+ levels and extracellular acidification rates, with EC50 values of approximately 1 microM. Both the acetylcholine muscarinic receptor antagonist atropine and the M3 muscarinic receptor-preferring antagonist 4-diphenylacetoxy-N-methylpiperidine (4-DAMP) inhibited the effects of CCh, three orders of magnitude more potently than the selective M2 muscarinic receptor antagonist, methoctramine. These data indicate the dominant role of M3 receptors in guinea-pig bladder but fail to show clear evidence of any functional role for M2 receptors. Since this finding agrees with a number of other studies using in vivo and in vitro models (1), cell suspensions such as these may prove to be simple tools for the pharmacological study of urinary bladder smooth muscle tissue.
Ikeda
K
K
Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd, Tsukuba, Ibaraki, Japan. ikedak@yamanouchi.co.jp
Kobayashi
S
S
Suzuki
M
M
Miyata
K
K
Yamada
T
T
Honda
K
K
D000818:Animals; D015374:Biosensing Techniques; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000378:metabolism*; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D016257:Fura-2; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008297:Male; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic / Q000502:physiology*; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D005456:Fluorescent Dyes; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic; D002217:Carbachol; D002118:Calcium; D016257:Fura-2