Purification and characterization of the overexpressed Aspergillus oryzae xylanase, XynF1.
The Aspergillus oryzae xynF1 gene coding for a xylanase, XynF1, was successfully overexpressed under the strong A. oryzae TEF1 gene promoter. The high-XynF1-producing transformant secreted about 180 mg/l of XynF1 in the glucose-containing medium. The overexpressed XynF1 was purified by only one chromatographic step. The purified XynF1 had a molecular mass of 35.0 kDa, a pH optimum of 5.0, and a temperature optimum of 60 degrees C.
Kitamoto
N
N
Food Research Institute, Aichi Prefectural Government, Nagoya, Japan.
Yoshino
S
S
Ohmiya
K
K
Tsukagoshi
N
N
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D015139:Blotting, Southern; D004591:Electrophoresis, Polyacrylamide Gel; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D013696:Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase