A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs.
We report the isolation of a novel soybean gene, Msg, which is highly expressed in developing soybean pods. The gene shows significant homology to a family of fruit- and flower-specific genes, designated the major latex protein (MLP) homologues, so far reported in only a few species and whose functions are unknown. The MLPs are more distantly related to a group of pathogenesis-related proteins (IPR or PR-10) whose functions are likewise unknown. This is the first report of a MLP homologue in a plant for which there is already an IPR-protein reported. We performed an analysis of the Msg promoter with 14 different promoter fragments ranging from 0.65 kb to 2.26 kb, fused to the uidA (GUS) gene. High transient expression was obtained with all the constructs upon particle bombardment in soybean and green bean pods. Stable Arabidopsis transformants were obtained with the Agrobacterium vacuum infiltration method. The promoter is fully active in Arabidopsis only in plants transformed with the 2.26 kb fragment promoter, expressing GUS in nectaries, nodes, short style and in guard cells of the silique, pedicel and stem but not in mature leaves. Surprisingly, the proximal 650 bp TATA-containing region cannot function on its own in Arabidopsis and can be deleted without a change in expression pattern in both Arabidopsis and soybean. Thus, tissue-specific regions of the complex Msg promoter reside in the distal 5' regions upstream of a dispensable TATA box in contrast to many examples of tissue-specific elements that reside much closer to the TATA box.
Strömvik
M V
MV
Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana 61801, USA.
Sundararaman
V P
VP
Vodkin
L O
LO
D000595:Amino Acid Sequence; D017360:Arabidopsis; D019677:Artificial Gene Fusion; D020029:Base Pairing; D001483:Base Sequence; D015870:Gene Expression; D017343:Genes, Plant; D015698:Genomic Library; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D018749:RNA, Plant; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics*; D014170:Transformation, Genetic
D012333:RNA, Messenger; D018749:RNA, Plant; D005966:Glucuronidase