Isolation of intracytoplasmic membrane from the methanotrophic bacterium Methylomicrobium album BG8.
Methane-oxidizing bacteria, including Methylomicrobium album BG8, form an intracytoplasmic membrane in addition to the cytoplasmic and outer membranes of the cell envelope. Techniques to isolate the intracytoplasmic membrane of M. album BG8 were developed. An intracytoplasmic membrane fraction was separated from a cell envelope fraction on the basis of sedimentation velocity in sucrose density gradients. Proteins associated with the particulate methane monooxygenase were found in both membrane fractions.
Brantner
C A
CA
Department of Biological Sciences and Great Lakes WATER Institute, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, USA.
Buchholz
L A
LA
Remsen
C C
CC
Collins
M L
ML
D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003300:Copper / Q000032:analysis / Q000737:chemistry; D007425:Intracellular Membranes / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry; D008772:Methylococcaceae / Q000166:cytology*; D010105:Oxygenases / Q000032:analysis / Q000737:chemistry
D008565:Membrane Proteins; D003300:Copper; D010105:Oxygenases; C021555:methane monooxygenase