Cell-autonomous regulation of cell and organ growth in Drosophila by Akt/PKB.
Organismal size is determined by a tightly regulated mechanism that coordinates cell growth, cell proliferation and cell death. The Drosophila insulin receptor/Chico/Dp110 pathway regulates cell and organismal size. Here we show that genetic manipulation of the phosphoinositide-3-OH-kinase-dependent serine/threonine protein kinase Akt (protein kinase B) during development of the Drosophila imaginal disc affects cell and organ size in an autonomous manner. Ectopic expression of Akt does not affect cell-fate determination, apoptosis or proliferation rates in imaginal discs. Thus, Akt appears to stimulate intracellular pathways that specifically regulate cell and compartment size independently of cell proliferation in vivo.
Verdu
J
J
Howard Hughes Medical Institute and the Department of Medicine, 415 Curie Boulevard, Clinical Research Building, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Buratovich
M A
MA
Wilder
E L
EL
Birnbaum
M J
MJ
D000818:Animals; D017209:Apoptosis; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D019070:Cell Lineage; D048429:Cell Size; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D005123:Eye / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000378:metabolism; D005434:Flow Cytometry; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D010641:Phenotype; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D011972:Receptor, Insulin / Q000378:metabolism; D014170:Transformation, Genetic; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000378:metabolism
D029721:Drosophila Proteins; D007328:Insulin; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D011972:Receptor, Insulin; C494917:Akt1 protein, Drosophila