Immunohistochemical detection of PGL-1, LAM, 30 kD and 65 kD antigens in leprosy infected paraffin preserved skin and nerve sections.
A panel of lipid, carbohydrate and protein antibodies were optimized for use in detecting M. leprae antigens in paraffin embedded material. Skin and nerve biopsies from 13 patients across the leprosy spectrum were studied. All antibodies detected antigen in tissues with a BI > 1. Phenolic-glycolipid was not detected in bacteriologically negative tissue but lipoarabinomanan (LAM) and protein antigens were detected. Staining with LAM was strongest and gave least background. The transfer of this immunohistochemical technique to paraffin embedded material will allow examination of tissue with better morphology and from clinics without access to tissue freezing facilities.
Van den Bos
I C
IC
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK.
Khanolkar-Young
S
S
Das
P K
PK
Lockwood
D N
DN
D000911:Antibodies, Monoclonal / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D018834:Chaperonin 60; D018833:Chaperonins / Q000032:analysis; D046508:Culture Techniques; D005260:Female; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000276:immunology / Q000473:pathology*; D008070:Lipopolysaccharides / Q000032:analysis; D008264:Macrophages / Q000737:chemistry; D008297:Male; D010525:Peripheral Nerves / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D018834:Chaperonin 60; D006017:Glycolipids; D008070:Lipopolysaccharides; C074119:heat-shock protein 65, Mycobacterium; C050016:lipoarabinomannan; C045169:phenolic glycolipid I, Mycobacterium leprae; C071448:antigen 85B, Mycobacterium leprae; D018833:Chaperonins