title: Phylogeny in the genus Hordeum based on nucleotide sequences closely linked to the vrs1 locus (row number of spikelets).
abstract: The phylogenetic relationship between four basic genomes designated H, I, Xa, and Xu in the genus Hordeum was studied using a nuclear DNA sequence. The sequence, cMWG699, is single copy in the H. vulgare genome, and tightly linked to the vrs1 locus which controls two- and six-rowed spikes. DNA fragments homologous to cMWG699 were amplified from diploid Hordeum species and the nucleotide sequences were determined. A phylogeny based on both base substitutions and an insertion-deletion event showed that the H- and Xa-genome groups are positioned in one monophyletic group indicating that the Xa-genome taxa should be included in the H-genome group. The large H-genome group is highly homogeneous. The I and Xu genomes are distinctly separated from H and Xa, and form sister groups. Another phylogeny pattern based on data excluding the insertion-deletion gave a result that the Xa genome forms a sister group to the H-genome group. The difference between the H and Xa genomes was affected only by a single base insertion-deletion event, thus the H and Xa genomes are likely to be closely related. The I and Xu genomes were again distinctly separated from the H and Xa genomes.
authors.affiliation: Department of Plant Breeding Research, Swedish University of Agricultural Sciences, Svalöv, Sweden. takao@abr.affrc.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Komatsuda
authors.forename: K
authors.initials: K
authors.lastname: Tanno
authors.forename: B
authors.initials: B
authors.lastname: Salomon
authors.forename: T
authors.initials: T
authors.lastname: Bryngelsson
authors.forename: R
authors.initials: R
authors.lastname: von Bothmer
mesh_terms: D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant