title: Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle.
abstract: Two enzyme-linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of 0.1 ng homidium per mL serum. Homidium levels were measurable in serum of cattle for over 2 months following administration of a single intramuscular (i.m.) dose at 1 mg/kg bodyweight. The level of sensitivity afforded by these assays makes them potentially useful tools in the pharmacokinetic evaluation of homidium and for investigating drug resistance or causes of drug failure. Assay 2 was chosen as being most suitable for further studies.
authors.affiliation: Radioisotope Laboratory, Kenya Trypanosomiasis Research Institute (KETRI), Kikuyu, Kenya.
authors.forename: G A
authors.initials: GA
authors.lastname: Murilla
authors.forename: M C
authors.initials: MC
authors.lastname: Eisler
authors.forename: A S
authors.initials: AS
authors.lastname: Peregrine
authors.forename: J M
authors.initials: JM
authors.lastname: Ndung'u
authors.forename: P H
authors.initials: PH
authors.lastname: Holmes
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004996:Ethidium / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D007273:Injections, Intramuscular; D008297:Male; D011786:Quality Control; D012756:Sheep; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D014352:Trypanosomiasis / Q000097:blood / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D014344:Trypanocidal Agents; D004996:Ethidium