ZNF74, a gene deleted in DiGeorge syndrome, is expressed in human neural crest-derived tissues and foregut endoderm epithelia.
DiGeorge syndrome (DGS) is a developmental disorder associated with large hemizygous deletions on chromosome 22q11.2. ZNF74 zinc finger gene is a candidate from the commonly deleted region. To address the potential involvement of ZNF74 in DGS, its human developmental expression pattern has been assessed. In situ hybridization on Carnegie Stage 18 embryos revealed that ZNF74 expression is limited to specific neural crest-derived tissues and neuroepithelium of the spinal cord as well as to foregut endoderm epithelia (esophagus and respiratory tract). Interestingly, ZNF74 expression was detected in the wall of the pulmonary artery and aorta and in the aortic valve, which are populated by neural crest-derived cells. This finding is significant, considering that DGS is believed to result from defective neural crest contributions and that outflow tract and aorticopulmonary septation defects are typical features of the DGS phenotype. Thus, the restricted expression of ZNF74 in structures affected in DGS suggests a role for this putative regulator of gene expression in aspects of the DGS phenotype.
Ravassard
P
P
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, CNRS-UMR C9923, Paris, France.
Côté
F
F
Grondin
B
B
Bazinet
M
M
Mallet
J
J
Aubry
M
M
D001011:Aorta / Q000196:embryology / Q000378:metabolism; D001021:Aortic Valve / Q000196:embryology / Q000378:metabolism; D019070:Cell Lineage; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D004062:DiGeorge Syndrome / Q000235:genetics* / Q000473:pathology; D005314:Embryonic and Fetal Development / Q000235:genetics; D004707:Endoderm / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005326:Fetal Proteins / Q000096:biosynthesis / Q000235:genetics*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000378:metabolism*; D009928:Organ Specificity; D010641:Phenotype; D011651:Pulmonary Artery / Q000196:embryology / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
D005326:Fetal Proteins; D051741:Kruppel-Like Transcription Factors; D016601:RNA-Binding Proteins; C100388:ZNF74 protein, human