Tryptophan-niacin metabolism in liver cirrhosis rat caused by carbon tetrachloride.
We investigated the change of tryptophan-niacin metabolism induced by carbon tetrachloride (CCl4) in rats with liver cirrhosis. The rats were injected with CCl4 (0.5 or 1 mL of 50% olive oil solution/kg body weight) twice a week for 1 or 2 mo and given phenobarbital water simultaneously. The urinary excretions of nicotinamide (Nam) and its metabolites were assayed. As the result, the urinary excretion of Nam, N1-methyl-4-pyridone-3-carboxamide (4-Py), Nam + N1-methylnicotinamide (MNA) + N1-methyl-2-pyridone-5-carboxamide (2-Py) + 4-Py was lower in the CCl4-treated groups than in the non-treated group (control) regardless of the experimental period (1 mo and 2 mo) or dosing amount of CCl4 (0.5 and 1 mL). Moreover, we investigated which pathway of tryptophan-niacin metabolism was affected in CCl4-treated rat. As the result, the possibility that the MNA-->4-Py reaction is inhibited by CCl4 treatment was suggested in this experiment.
Egashira
Y
Y
Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Japan. egashira@midori.h.chiba-u.ac.jp
Isagawa
A
A
Komine
T
T
Yamada
E
E
Ohta
T
T
Shibata
K
K
Sanada
H
H
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D009525:Niacin / Q000378:metabolism*; D009536:Niacinamide / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D014364:Tryptophan / Q000378:metabolism*; D015430:Weight Gain
D009536:Niacinamide; D009525:Niacin; D014364:Tryptophan; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase