title: Selection of refractory and permissive strains of Aedes triseriatus (Diptera: Culicidae) for transovarial transmission of La Crosse virus.
abstract: The genetic basis of transovarial transmission of La Crosse virus in Aedes triseriatus (Say) was investigated through selection experiments on 2 mosquito strains. One strain was subject to selection for transovarial transmission refractoriness, the other for permissiveness to transovarial transmission. Response to selection for a low filial infection rate was rapid, decreasing from 18 to 3% in 3 generations. However, no response to selection for permissiveness was observed in the other strain; the average filial infection rates through 4 generations fluctuated between 25 and 40%. By contrast, the transovarial transmission rate in both strains showed a consistent response to selection in both directions. These patterns are consistent with a model in which transovarial transmission is controlled by a single genetic locus and permissiveness is conditioned by dominant alleles; whereas the filial infection rate is nongenetic and influenced by stochastic factors in the mosquito and virus.
authors.affiliation: Arthropod-Borne and Infectious Diseases Laboratory, Colorado State University, Fort Collins 80523, USA.
authors.forename: D H
authors.initials: DH
authors.lastname: Graham
authors.forename: J L
authors.initials: JL
authors.lastname: Holmes
authors.forename: S
authors.initials: S
authors.lastname: Higgs
authors.forename: B J
authors.initials: BJ
authors.lastname: Beaty
authors.forename: W C
authors.initials: WC
authors.lastname: Black
mesh_terms: D000330:Aedes / Q000235:genetics / Q000821:virology*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004670:Encephalitis, California / Q000635:transmission*; D005260:Female; D018062:La Crosse virus / Q000502:physiology*; D007814:Larva / Q000821:virology; D008297:Male; D010053:Ovary / Q000821:virology; D013045:Species Specificity