B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.
The B7 family members B7-1 and B7-2 interact with CD28 and constitute an essential T-cell co-stimulatory pathway in the initiation of antigen-specific humoral and cell-mediated immune response. Here, we describe a third member of the B7 family, called B7-H1 that does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). Ligation of B7-H1 co-stimulated T-cell responses to polyclonal stimuli and allogeneic antigens, and preferentially stimulated the production of interleukin-10. Interleukin-2, although produced in small amounts, was required for the effect of B7-H1 co-stimulation. Our studies thus define a previously unknown co-stimulatory molecule that may be involved in the negative regulation of cell-mediated immune responses.
Dong
H
H
Department of Immunology, Mayo Graduate and Medical Schools, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905, USA.
Zhu
G
G
Tamada
K
K
Chen
L
L
D000595:Amino Acid Sequence; D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000096:biosynthesis / Q000378:metabolism*; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D018122:B7-1 Antigen; D018076:DNA, Complementary; D007376:Interleukin-2; D011994:Recombinant Proteins; D016753:Interleukin-10