title: Comparative chromosome painting between marsupial orders: relationships with a 2n = 14 ancestral marsupial karyotype.
abstract: A 2n = 14 karyotype is shared by some species in each of the marsupial orders in Australian and American superfamilies, suggesting that the ancestral marsupial chromosome complement was 2n = 14. We have used chromosome painting between distantly related marsupial species to discover whether genome arrangements in 2n = 14 species in two Australian orders support this hypothesis. Cross-species chromosome painting was used to investigate chromosome rearrangements between a macropodid species Macropus eugenii (2n = 16) and a wombat species in a different suborder (Lasiorhinus latifrons, 2n = 14), and a dasyurid species in a different order (Sminthopsis macroura, 2n = 14). We demonstrate that many chromosome regions are conserved between all three species, and deduce how the similar 2n = 14 karyotypes of species in the two orders are related to a common ancestral 2n = 14 karyotype.
authors.affiliation: Department of Biochemistry and Genetics, La Trobe University, Bundoora, VIC, Australia.
authors.forename: A A
authors.initials: AA
authors.lastname: De Leo
authors.forename: N
authors.initials: N
authors.lastname: Guedelha
authors.forename: R
authors.initials: R
authors.lastname: Toder
authors.forename: L
authors.initials: L
authors.lastname: Voullaire
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson-Smith
authors.forename: P C
authors.initials: PC
authors.lastname: O'Brien
authors.forename: J A
authors.initials: JA
authors.lastname: Graves
mesh_terms: D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D020223:Chromosome Painting; D017931:DNA Primers; D007621:Karyotyping; D008394:Marsupialia / Q000235:genetics*; D013045:Species Specificity; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D017931:DNA Primers