Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons.
Two tetrodotoxin-resistant (TTX-R) voltage-gated sodium channels, SNS and NaN, are preferentially expressed in small dorsal root ganglia (DRG) and trigeminal ganglia neurons, most of which are nociceptive, of rat and mouse. We report here the sequence of NaN from human DRG, and demonstrate the presence of two TTX-R currents in human DRG neurons. One current has physiological properties similar to those reported for SNS, while the other displays hyperpolarized voltage-dependence and persistent kinetics; a similar TTX-R current was recently identified in DRG neurons of sns-null mouse. Thus SNS and NaN channels appear to produce different currents in human DRG neurons.
Dib-Hajj
S D
SD
Department of Neurology LCI 707, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA.
Tyrrell
L
L
Cummins
T R
TR
Black
J A
JA
Wood
P M
PM
Waxman
S G
SG
D000595:Amino Acid Sequence; D004594:Electrophysiology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D062559:NAV1.8 Voltage-Gated Sodium Channel; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015222:Sodium Channels / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013779:Tetrodotoxin / Q000633:toxicity
D062559:NAV1.8 Voltage-Gated Sodium Channel; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009479:Neuropeptides; C568317:SCN10A protein, human; C491886:SCN11A protein, human; C568318:Scn10a protein, mouse; C568319:Scn10a protein, rat; C491885:Scn11a protein, mouse; D015222:Sodium Channels; D013779:Tetrodotoxin