Affinity extraction of BSA with reversed micellar system composed of unbound Cibacron Blue.
Affinity Cibacron Blue 3GA (CB) dye in aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide (CTAB). The bovine serum albumin (BSA) transfer to the reverse micelles increases significantly in a wide range of pH by the addition of a small amount of CB ( approximately 1.0-7.0% of the total surfactant concentration) to the aqueous phase. For pH < pI, the selectivity can be significantly improved with the presence of affinity CB because no BSA was extracted in the absence of CB. For backward extraction of BSA from the micellar phase with stripping aqueous solution, the addition of 2-propanol to the aqueous phase can recover almost all BSA (98.5%) extracted into the reverse micelles.
Zhang
T
T
Laboratory of Separation Science and Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China. lsse@home.icm.ac.cn
Liu
H
H
Chen
J
J
D000818:Animals; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D004396:Coloring Agents; D003902:Detergents; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008823:Micelles; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D055672:Static Electricity; D014227:Triazines
D002593:Cetrimonium Compounds; D004396:Coloring Agents; D003902:Detergents; D007202:Indicators and Reagents; D008823:Micelles; D014227:Triazines; D012710:Serum Albumin, Bovine; C012842:Cibacron Blue F 3GA; D000077286:Cetrimonium