Mn-porphyrin derivatives as an antioxidant for medical devices.
It is well known that reactive oxygen species such as O*2- and H2O2 induce the biodegradation or cracking of medical devices in vivo or that they are released from inflammatory cells activated by devices to oxidize low-density lipoprotein. Therefore, the development of a novel antioxidant is required to eliminate the reactive oxygen species. In this paper, we report that Mn-porphyrin derivatives containing a macromolecular Mn-porphyrin are relatively stable compounds that can eliminate O*2- and/or H2O2. The dismutation of O*2- in the porphyrins was determined using a cytochrome c-assay by the xanthine/xanthine oxidase system and using the stopped-flow kinetic analysis technique. The possibility of porphyrins as scavengers of H2O2 was evaluated by in situ measurement with a Clark electrode. As a result, it has been found that Mn-porphyrin derivatives may be a vastly better scavenger of reactive oxygen species in vivo.
Ohse
T
T
Department of Applied Chemistry, Tokyo Metropolitan University, Hachioji, Japan. kawakami-hiroyoshi@c.metro-u.ac.jp
Kawakami
H
H
Morita
A
A
Nagaoka
S
S
D000975:Antioxidants / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D008422:Materials Testing; D008665:Metalloporphyrins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D012995:Solubility; D013481:Superoxides / Q000378:metabolism; D014867:Water
D000975:Antioxidants; D001672:Biocompatible Materials; D016166:Free Radical Scavengers; D008665:Metalloporphyrins; D017382:Reactive Oxygen Species; D014867:Water; D013481:Superoxides; D006861:Hydrogen Peroxide