title: Increased risk of antibody-mediated rejection of reduced-size liver allografts.
abstract: Because of the shortage of liver allografts in children, transplantation of reduced-size liver allografts from adult cadaveric donors or living, related donors is being done more frequently. Reduced-size liver allografts may be used in cases of ABO incompatibility and T-cell warm cross-match positivity. This experimental study in inbred rats was undertaken to determine if reduced-size liver allografts are more sensitive to antibody-mediated rejection than full-size liver allografts. Brown-Norway (BN) (RT1(n)) rats were sensitized by three successive skin grafts at 10-day intervals. Then orthotopic Lewis (LEW) (RT1(1)) liver grafts were transplanted into these BN rats. Full-size liver allografts were compared with reduced-size liver allografts (70% of donor liver). Control groups were composed of full-size and/or reduced-size isografts. Titers of specific antibodies were assayed using a complement-dependent assay before and after orthotopic liver transplantation. Histological and immunofluorescence studies (IgG, IgM, C(3), and fibrinogen deposits) were assessed. Recipients of reduced-size liver allografts died of hyperacute rejection at 36.6 +/- 4.1 h, significantly earlier than recipients receiving full-size liver allografts, which died of accelerated acute rejection at 259.2 +/- 25.2 h (P < 0.001). Either full-size or reduced-size isograft recipients survived indefinitely. A decrease in the titers of donor-specific antibodies was observed in both groups of animals. Slight deposits of IgG, IgM, C(3), and fibrinogen were observed in recipients of reduced-size liver allografts, whereas larger deposits were observed in recipients of full-size liver allografts. Our data demonstrate that there is an increased risk of antibody-mediated rejection of reduced-size liver allografts in sensitized recipients. This may have important clinical implications for partial liver grafting in cases of ABO incompatibility and T-cell warm cross-match positivity.
authors.affiliation: Groupe de recherche de Chirurgie Hépatobiliaire, Hôpital Paul Brousse, Villejuif, France.
authors.forename: I
authors.initials: I
authors.lastname: Astarcioglu
authors.forename: R
authors.initials: R
authors.lastname: Cursio
authors.forename: M
authors.initials: M
authors.lastname: Reynes
authors.forename: J
authors.initials: J
authors.lastname: Gugenheim
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D006084:Graft Rejection; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation / Q000276:immunology* / Q000401:mortality; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D012306:Risk; D014184:Transplantation, Homologous
chemical_list: D000906:Antibodies