A kinetic epoxidation assay for chloroperoxidase.
Chloroperoxidase exhibits a wide variety of enantioselective epoxidation reactions. Until now, the epoxidation activities have been mainly evaluated using elaborate gas chromatographic methods. This paper reports a rapid and convenient spectrophotometric assay for CPO. The disappearance of indene by catalytic epoxidation is monitored at 250 nm and this is used as an index of enzyme activity. This method will prove to be highly useful in large-scale screening of mutants.
Manoj
K M
KM
Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Yi
X
X
Rai
G P
GP
Hager
L P
LP
D002711:Chloride Peroxidase / Q000032:analysis* / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007192:Indenes / Q000737:chemistry; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet; D020122:tert-Butylhydroperoxide / Q000737:chemistry
D004852:Epoxy Compounds; D007192:Indenes; C093581:indene; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D002711:Chloride Peroxidase