title: Sec-independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein.
abstract: In Escherichia coli, transmembrane translocation of proteins can proceed by a number of routes. A subset of periplasmic proteins are exported via the Tat pathway to which proteins are directed by N-terminal "transfer peptides" bearing the consensus (S/T)RRXFLK "twin-arginine" motif. The Tat system involves the integral membrane proteins TatA, TatB, TatC, and TatE. Of these, TatA, TatB, and TatE are homologues of the Hcf106 component of the DeltapH-dependent protein import system of plant thylakoids. Deletion of the tatB gene alone is sufficient to block the export of seven endogenous Tat substrates, including hydrogenase-2. Complementation analysis indicates that while TatA and TatE are functionally interchangeable, the TatB protein is functionally distinct. This conclusion is supported by the observation that Helicobacter pylori tatA will complement an E. coli tatA mutant, but not a tatB mutant. Analysis of Tat component stability in various tat deletion backgrounds shows that TatC is rapidly degraded in the absence of TatB suggesting that TatC complexes, and is stabilized by, TatB.
authors.affiliation: Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: F
authors.initials: F
authors.lastname: Sargent
authors.forename: N R
authors.initials: NR
authors.lastname: Stanley
authors.forename: B C
authors.initials: BC
authors.lastname: Berks
authors.forename: T
authors.initials: T
authors.lastname: Palmer
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001692:Biological Transport; D004926:Escherichia coli / Q000378:metabolism*; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D015696:Gene Products, tat; D006864:Hydrogenase