title: The distribution of type VI collagen in the developing tissues of the bovine femoral head.
abstract: Type VI collagen appears central to the maintenance of tissue integrity. In adult articular cartilage, type VI collagen is preferentially localised in the chondron where it may be involved in cell attachment. In actively remodelling developing cartilage, the distribution is less certain. We have used confocal immunohistochemistry and in situ hybridisation to investigate type VI collagen distribution in third trimester bovine proximal femoral epiphyses. In general, type VI collagen immunofluorescence was concentrated in the chondrocyte pericellular matrix, with staining intensity strongest in regions which persist to maturity and weakest in regions that remodel during development. Type VI collagen was also present in cartilage canals. In the growth plate and around the secondary centre of ossification, the intensity of type VI collagen stain rapidly decreased with chondrocyte maturation and was absent at hypertrophy, except where canal branches penetrated the growth plate and stain was retained around the adjacent chondrocytes. In situ hybridisation confirmed the presence of type VI collagen mRNA in cartilage canal mesenchymal cells but the signal was low in chondrocytes, suggesting minimal levels of synthesis and turnover. The results are consistent with a role for type VI collagen in stabilising the extracellular matrix during development.
authors.affiliation: Department of Anatomy, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
authors.forename: A F
authors.initials: AF
authors.lastname: Sherwin
authors.forename: D H
authors.initials: DH
authors.lastname: Carter
authors.forename: C A
authors.initials: CA
authors.lastname: Poole
authors.forename: J A
authors.initials: JA
authors.lastname: Hoyland
authors.forename: S
authors.initials: S
authors.lastname: Ayad
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000196:embryology / Q000378:metabolism; D002417:Cattle; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D005270:Femur Head / Q000196:embryology* / Q000378:metabolism; D006132:Growth Plate / Q000196:embryology / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D011817:Rabbits
chemical_list: D003094:Collagen