Preliminary study of the incidence of disomy in sperm fractions after MicroSort flow cytometry.
Using triple colour fluorescent in-situ hybridization (FISH) we have evaluated, on a blind basis, the disomy level for chromosome 21 and the sex chromosomes in flow cytometric sorted sperm samples. There were no statistically significant differences in the disomy rates when comparing the sorted samples (either for X- or Y-bearing spermatozoa) with non-sorted samples. There were no diploid spermatozoa observed in any sample group after MicroSort processing.
Vidal
F
F
Unitat de Biologia Cellular, Facultat de Ciències, Universitat Autònoma Barcelona, 08193-Barcelona, Spain.
Blanco
J
J
Fugger
E F
EF
Keyvanfar
K
K
Norton
M
M
Schulman
J D
JD
Egozcue
J
J
D000328:Adult; D002469:Cell Separation; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D004171:Diploidy; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015994:Incidence; D008297:Male; D012730:Sex Chromosomes / Q000235:genetics; D013094:Spermatozoa / Q000502:physiology*