Cholecystokinin CCK(B) receptor mRNA isoforms: expression in schizophrenic brains.
In the light of earlier findings of reduced cholecystokinin (CCK) peptide and CCK mRNA levels in the cerebral cortex, we have used in situ hybridization to examine possible regulation of mRNAs coding for two isoforms of the CCK(B) receptor in frontal cortex (Brodmann's area 10) of schizophrenic patients. The hybridizations revealed a 51% decrease of the full length CCK(B) receptor mRNA in the outer layers (II-III) of the frontal cortex. The corresponding alterations for the truncated isoform were a 65% reduction in the outer layers and a 62% reduction in the inner layers (IV-VI) of the frontal cortex. This strengthens the hypothesis that CCKergic transmission in this part of the brain is involved in the pathophysiology of schizophrenia.
Zachrisson
O
O
Department of Clinical Neuroscience, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden.
de Belleroche
J
J
Wendt
K R
KR
Hirsch
S
S
Lindefors
N
N
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001345:Autoradiography; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D007536:Isomerism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000096:biosynthesis*; D012559:Schizophrenia / Q000378:metabolism*; D016610:Tissue Embedding
D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin