Antithrombotic activities of green tea catechins and (-)-epigallocatechin gallate.
The antithrombotic activities and mode of action of green tea catechins (GTC) and (-)-epigallocatechin gallate (EGCG), a major compound of GTC, were investigated. Effects of GTC and EGCG on the murine pulmonary thrombosis in vivo, human platelet aggregation in vitro, and ex vivo, and coagulation parameters were examined. GTC and EGCG prevented death caused by pulmonary thrombosis in mice in vivo in a dose-dependent manner. They significantly prolonged the mouse tail bleeding time of conscious mice. They inhibited adenosine diphosphate- and collagen-induced rat platelet aggregation ex vivo in a dose-dependent manner. GTC and EGCG inhibited ADP-, collagen-, epinephrine-, and calcium ionophore A23187-induced human platelet aggregation in vitro dose dependently. However, they did not change the coagulation parameters such as activated partial thromboplastin time, prothrombin time, and thrombin time using human citrated plasma. These results suggest that GTC and EGCG have the antithrombotic activities and the modes of antithrombotic action may be due to the antiplatelet activities, but not to anticoagulation activities.
Kang
W S
WS
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Lim
I H
IH
Yuk
D Y
DY
Chung
K H
KH
Park
J B
JB
Yoo
H S
HS
Yun
Y P
YP
D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001760:Bleeding Time; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003094:Collagen / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011655:Pulmonary Embolism / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry*
D005343:Fibrinolytic Agents; D007476:Ionophores; D010975:Platelet Aggregation Inhibitors; D013662:Tea; D000001:Calcimycin; D000244:Adenosine Diphosphate; D002392:Catechin; D003094:Collagen; C045651:epigallocatechin gallate; D001241:Aspirin; D002118:Calcium; D004837:Epinephrine