title: The evolutionary transition from monomeric to oligomeric proteins: tools, the environment, hypotheses.
abstract: Recently, renewed interest in the evolution of oligomeric proteins has seen new approaches explored and new hypotheses proposed. The model systems chosen are generally made up of pairs of homologous proteins, each composed of a monomer and a dimeric counterpart, but the question has been also approached by comparing statistically significant structural patterns in sets of monomeric and oligomeric proteins. Here the tools of genetics and chemistry potentially available to the evolution of oligomeric proteins are discussed, as well as the possible effects of environments on the early attempts to oligomerization. Traces of an ancestral monomeric status of oligomers may be detected in the significant presence of polar and charged residues at intersubunit interfaces, and by the recognition that, besides the hydrophobic effect, a 'hydrophilic' effect has also had a role in the construction of these interfaces. The traditional 'mutation' model is described and found to be based on a hierarchy of mutations, crowned by a 'primary' mutation, one that could prime oligomerization by irreversibly altering the structure of an ancestral monomer. The mechanism of oligomerization based on the exchange or 'swap' of structural elements between monomers is discussed. The possibility is also discussed that the main steps in the folding pathway of an oligomeric protein reiterate the main steps in its evolution.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, University of Naples Federico II, Italy. dalessio@unina.it
authors.forename: G
authors.initials: G
authors.lastname: D'Alessio
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D004247:DNA / Q000235:genetics; D019281:Dimerization; D019143:Evolution, Molecular; D020440:Gene Duplication; D006454:Hemoglobins / Q000737:chemistry; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D006454:Hemoglobins; D011506:Proteins; D004247:DNA