Genetic variation between susceptible and non-susceptible snails to Schistosoma infection using random amplified polymorphic DNA analysis (RAPDs).
Susceptibility of snails to infection by certain trematodes and their suitability as hosts for continued development has been a bewildering problem in host-parasite relationships. The present work emphasizes our interest in snail genetics to determine what genes or gene products are specifically responsible for susceptibility of snails to infection. High molecular weight DNA was extracted from both susceptible and non-susceptible snails within the same species Biomphalaria tenagophila. RAPD was undertaken to distinguish between the two types of snails. Random primers (10 mers) were used to amplify the extracted DNA by the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The results suggest that RAPD represents an efficient means of genome comparison, since many molecular markers were detected as genetic variations between susceptible and non-susceptible snails.
Abdel-Hamid
A H
AH
Medicinal Chemistry Department, National Research Center, Cairo, Egypt.
de Molfetta
J B
JB
Fernandez
V
V
Rodrigues
V
V
D000818:Animals; D001700:Biomphalaria / Q000235:genetics* / Q000469:parasitology*; D018509:DNA, Helminth / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D006790:Host-Parasite Interactions / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012550:Schistosoma mansoni / Q000502:physiology*
D018509:DNA, Helminth