[Immunology in the medical practice. XXIV. Expression of antibodies on bacteriophages; possibilities for in vitro production of human antibodies for any desired application].
Although poly- and monoclonal antibodies are successfully applied in research, an expected clinical breakthrough of these reagents so far has not occurred. This can mainly be explained by the animal origin of antibodies, which may lead to a deleterious immune response upon therapeutic use in humans. Moreover, it has been technically demanding to alter the desired affinity, format and effector functions of existing antibodies. Currently, antibody phage-display technology, through construction of large and highly diverse antibody libraries, completely by-passing the immune system, enables the isolation of human antibodies, which can be engineered for every desired application.
de Bruïne
A P
AP
Academisch Ziekenhuis, afd. Pathologie, Onderzoeksinstituut Groei en Ontwikkeling (CESAME), Maastricht. adb@lpat.azm.nl
Arends
J W
JW
Hoogenboom
H R
HR
D000911:Antibodies, Monoclonal / Q000009:adverse effects / Q000096:biosynthesis* / Q000276:immunology*; D001435:Bacteriophages / Q000276:immunology*; D020650:Combinatorial Chemistry Techniques; D006801:Humans; D007116:Immunization, Passive / Q000009:adverse effects / Q000379:methods* / Q000639:trends*; D008967:Molecular Biology / Q000379:methods; D019151:Peptide Library
D000911:Antibodies, Monoclonal; D019151:Peptide Library
Immunologie in de medische praktijk. XXIV. Expressie van antistoffen op bacteriofagen; mogelijkheden voor in-vitroproductie van humane antistoffen met elke gewenste specificiteit.