Cartilage degradation by hyaluronate lyase and chondroitin ABC lyase: a MALDI-TOF mass spectrometric study.
Matrix-assisted laser desorption ionization and time-of-flight mass spectrometry (MALDI-TOF MS) has been used to investigate degradation products of two selected polysaccharides of cartilage (chondroitin sulfate and hyaluronic acid). Testicular hyaluronate lyase and chondroitin ABC lyase were used for enzymic digestion of both polysaccharides as well as of cartilage specimens. Polysaccharide solutions and cartilage supernatants were assayed by positive and negative MALDI-TOF MS. Especially chondroitin ABC lyase produced high amounts of digestion products (unsaturated di- and tetrasaccharides) from polysaccharides as well as from cartilage, clearly monitored by MALDI-TOF MS. It is concluded that MALDI-TOF MS provides a precise and fast tool for the determination of oligosaccharides since no previous derivatization is required.
Schiller
J
J
Institute of Medical Physics and Biophysics, University of Leipzig, Germany. schij@server3.medizin.uni-leipzig.de
Arnhold
J
J
Benard
S
S
Reichl
S
S
Arnold
K
K
D000818:Animals; D002356:Cartilage / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D019765:Chondroitin ABC Lyase / Q000378:metabolism*; D008297:Male; D009666:Nose; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D011514:Proteus vulgaris / Q000201:enzymology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013737:Testis / Q000201:enzymology
D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase; D019765:Chondroitin ABC Lyase