Matrix-mixed culture: new methodology for chondrocyte culture and preparation of cartilage transplants.
For cartilage engineering a variety of biomaterials were applied for 3-dimensional chondrocyte embedding and transplantation. In order to find a suitable carrier for the in vitro culture of chondrocytes and the subsequent preparation of cartilage transplants we investigated the feasibility of a combination of the well-established matrices fibrin and alginate. In this work human articular chondrocytes were embedded and cultured either in alginate, a mixture of alginate and fibrin, or in a fibrin gel after the extraction of the alginate component (porous fibrin gel) over a period of 30 days. Histomorphological analysis, electron microscopy, and immunohistochemistry were performed to evaluate the phenotypic changes of the chondrocytes, as well as the quality of the newly formed cartilaginous matrix. Our experiments showed that a mixture of 0.6% alginate with 4.5% fibrin promoted sufficient chondrocyte proliferation and differentiation, resulting in the formation of a specific cartilage matrix. Alginate served as a temporary supportive matrix component during in vitro culture and can be easily removed prior to transplantation. The presented tissue engineering method on the basis of a mixed alginate-fibrin carrier offers the opportunity to create stable cartilage transplants for reconstructive surgery.
Perka
C
C
Department of Orthopedics, University-Hospital Charité, Berlin, Germany. carsten.perka@charite.de
Spitzer
R S
RS
Lindenhayn
K
K
Sittinger
M
M
Schultz
O
O
D000464:Alginates; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D002358:Cartilage, Articular / Q000637:transplantation*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005337:Fibrin; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D007150:Immunohistochemistry; D008422:Materials Testing; D008854:Microscopy, Electron; D011509:Proteoglycans / Q000378:metabolism
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011509:Proteoglycans; D020723:Glucuronic Acid; D005337:Fibrin; D003094:Collagen