Determination of zolpidem in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats.
A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating zolpidem in rat serum microsamples (50 microl). The separation used a 2.1 mm I.D. reversed-phase OD-5-100 C18 column, 5 microm particle size with an isocratic mobile phase consisting of methanol-acetonitrile-26 mM sodium acetate buffer (adjusted to pH 2.0 with 40% phosphoric acid) containing 0.26 mM tetrabutylammonium phosphate (13:10:77, v/v/v). The detection limit was 3 ng/ml for zolpidem using an ultraviolet detector operated at 240 nm. The recovery was greater than 87% with analysis performed in 12 min. The method is simple, rapid, and applicable to pharmacokinetic studies of zolpidem after administering two intravenous bolus doses (1 and 4 mg/kg) in rats.
Wang
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Capital University of Medical Sciences, Department of Chemistry, School of Basic Sciences, Beijing, People's Republic of China.
Sun
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L
Lau
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CE
D000097:Acetonitriles; D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006993:Hypnotics and Sedatives / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D000432:Methanol; D008832:Microchemistry; D011725:Pyridines / Q000097:blood* / Q000493:pharmacokinetics*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D019346:Sodium Acetate; D000077334:Zolpidem
D000097:Acetonitriles; D002021:Buffers; D006993:Hypnotics and Sedatives; D011725:Pyridines; D019346:Sodium Acetate; D000077334:Zolpidem; D000432:Methanol; C032159:acetonitrile