title: Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion.
abstract: Following appropriate antigen-specific stimulation, CD4(+) and CD8(+) T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell surface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4(+) and CD8(+) T lymphocytes. The power of this technique is demonstrated here for HLA-A0201-restricted influenza matrix protein peptide 58-66-specific CD8(+) cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.
authors.affiliation: Miltenyi Biotec GmbH Bergisch Gladbach, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Brosterhus
authors.forename: S
authors.initials: S
authors.lastname: Brings
authors.forename: H
authors.initials: H
authors.lastname: Leyendeckers
authors.forename: R A
authors.initials: RA
authors.lastname: Manz
authors.forename: S
authors.initials: S
authors.lastname: Miltenyi
authors.forename: A
authors.initials: A
authors.lastname: Radbruch
authors.forename: M
authors.initials: M
authors.lastname: Assenmacher
authors.forename: J
authors.initials: J
authors.lastname: Schmitz
mesh_terms: D017951:Antigen Presentation; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D016207:Cytokines / Q000276:immunology*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*
chemical_list: D000941:Antigens; D016207:Cytokines; D011948:Receptors, Antigen, T-Cell