Development of an on-line monitoring system of human keratinocyte growth by image analysis and its application to bioreactor culture.
Human keratinocytes were cultured in serum-free medium for the purpose of on-line cell growth monitoring by image analysis. The validity of a process using a newly developed video microscopy system with image analysis for growth-rate monitoring in real time was verified by the measurement of the degree of confluence of keratinocytes in T-flasks and Petriperm dishes. The growth rate of keratinocytes was calculated subsequently from the linear relationship between average degree of confluence and cell concentration. This technique was applied to the culture in the bioreactor "KERATOR" in which a special video microscopy system using a CCD camera was built. The cell concentration evaluated by image analysis agreed well with that evaluated by conventional direct cell counting after enzymatic digestion, and the on-line monitoring of the specific growth rate allowed identification of both lag- and exponential-growth phases of the culture.
Kino-Oka
M
M
Department of Chemical Science and Engineering, Osaka University, Japan.
Prenosil
J E
JE
D019149:Bioreactors; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015603:Keratinocytes / Q000166:cytology*