title: Development of class-switched, affinity-matured monoclonal antibodies following a 7-day immunization schedule.
abstract: Previously we reported making high-affinity monoclonal antibodies (MAbs) 13 days after the onset of Repetitive Immunizations Multiple Sites (RIMMS) strategy. The Ig subclass variety and affinity of these antibodies suggested that maturational processes had already begun within draining lymph nodes. We now demonstrate that this diversity can in fact be captured as early as Day 7. In the work reported here, somatic fusion of immune lymphocytes isolated from peripheral lymph nodes resulted in the isolation of affinity-matured MAbs reactive with cytosine deaminase. This model further demonstrates and substantiates at a cellular level the rapid development and maturation of T-cell-dependent B-cell responses occurring within draining lymph nodes following antigen challenge.
authors.affiliation: Department of Molecular Sciences, Glaxo Wellcome, Research Triangle Park, NC 27709, USA.
authors.forename: J
authors.initials: J
authors.lastname: Bynum
authors.forename: J L
authors.initials: JL
authors.lastname: Andrews
authors.forename: B
authors.initials: B
authors.lastname: Ellis
authors.forename: F C
authors.initials: FC
authors.lastname: Kull
authors.forename: E A
authors.initials: EA
authors.lastname: Austin
authors.forename: K E
authors.initials: KE
authors.lastname: Kilpatrick
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000145:classification / Q000302:isolation & purification; D000915:Antibody Affinity; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D043525:Cytosine Deaminase; D006825:Hybridomas / Q000276:immunology; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification / Q000302:isolation & purification; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D009700:Nucleoside Deaminases / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase