title: Construction of a LukS-PV mutant of a staphylococcal Panton-Valentine leukocidin component having a high LukS-like function.
abstract: A 2-residue (D12I13) segment of LukS of a staphylococcal leukocidin component is an essential region for the hemolytic function of LukS towards rabbit erythrocytes in the presence of LukF. Here, we report that insertion of D, I, or AA residue(s) between A11 and E12 residues of LukS-PV, in which the 2-residue D12I13 segment in LukS was absent, confers the full LukS function on LukS-PV, which has only 4% hemolytic activity of that of LukS towards rabbit erythrocytes.
authors.affiliation: Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Shimatani
authors.forename: J
authors.initials: J
authors.lastname: Kaneko
authors.forename: T
authors.initials: T
authors.lastname: Tomita
authors.forename: Y
authors.initials: Y
authors.lastname: Kamio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001483:Base Sequence; D005098:Exotoxins; D006461:Hemolysis; D006801:Humans; D007956:Leukocidins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D011817:Rabbits; D013211:Staphylococcus aureus / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C108483:leukocidin S-component protein, Staphylococcus