Expression of testicular genes in haematological malignancies.
The gene expression of a new group of tumour antigens known as cancer/testis (CT) antigens is now well-recognized in some solid tumours. However, their expression in haematological malignancies remained unclear. In this study, we have used reverse transcription polymerase chain reaction and Southern blot analysis to examine the presence of transcripts for the three CT antigens, NY-ESO-1, SSX2 and SCP1 in haematological malignant cells. We found that transcripts for SCP1 could be detected in 10% of myeloma, 5.7% of acute myeloid leukaemia and 23% of chronic myeloid leukaemia. In contrast, NY-ESO-1 and SSX2 were not detected in any of the 107 tumour samples.
Lim
S H
SH
Department of Haematology, University of Wales College of Medicine, Cardiff, UK.
Austin
S
S
Owen-Jones
E
E
Robinson
L
L
D000951:Antigens, Neoplasm / Q000235:genetics*; D001854:Bone Marrow Cells / Q000502:physiology; D004268:DNA-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D019337:Hematologic Neoplasms / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000276:immunology; D008297:Male; D008565:Membrane Proteins; D009363:Neoplasm Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D013737:Testis; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012097:Repressor Proteins; C489753:SYCP1 protein, human; C093962:synovial sarcoma X breakpoint proteins