title: [Production of recombinant hIL-4delta2-a native isoform of human interleukin-4 in Escherichia coli cells].
abstract: Expression plasmids containing the synthetic gene hil-4 delta 2 was constructed to produce human interleukin-4 in Escherichia coli cells. Strains TG1 (pBTIL-4 delta 2) and BL21 (DE3) (pETIL-4 delta 2) produced the recombinant protein as inclusion bodies, and its production level was up to 30% of the total cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T cell proliferation, and this effect was enhanced by cyclosporin A.
authors.affiliation: Russian Federation State Research Center Genetika, Moscow, Russia. mashko@vnigen.msk.su
authors.forename: L R
authors.initials: LR
authors.lastname: Ptitsyn
authors.forename: S V
authors.initials: SV
authors.lastname: Smirnov
authors.forename: I B
authors.initials: IB
authors.lastname: Al'tman
authors.forename: N N
authors.initials: NN
authors.lastname: Samsonova
authors.forename: A V
authors.initials: AV
authors.lastname: Khodiakova
authors.forename: R N
authors.initials: RN
authors.lastname: Vasilenko
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D015847:Interleukin-4
vernacular_title: Produktsiia rekombinantnogo hIL-4delta2-prirodnoĭ izoformy interleĭkina-4 cheloveka, v kletkakh Esherichia coli.