title: The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations.
abstract: BACKGROUND
  The kinesin superfamily of microtubule-associated motor proteins are important for intracellular transport and for cell division in eukaryotes. Conventional kinesins have the motor domain at the N terminus of the heavy chain and move towards the plus end of microtubules. The ncd protein is necessary for chromosome segregation in meiosis. It belongs to a subfamily of kinesins that have the motor domain at the C terminus and move towards the minus end of microtubules.
  
  
  RESULTS
  The crystal structure of dimeric ncd has been obtained at 2.9 A resolution from crystals with the C222(1) space group, with two independent dimers per asymmetric unit. The motor domains in these dimers are not related by crystallographic symmetry and the two ncd dimers have significantly different conformations. An alpha-helical coiled coil connects, and interacts with, the motor domains.
  
  
  CONCLUSIONS
  The ncd protein has a very compact structure, largely due to extended interactions of the coiled coil with the head domains. Despite this, we find that the overall conformation of the ncd dimer can be rotated by as much as 10 degrees away from that of the twofold-symmetric archetypal ncd. The crystal structures of conventional kinesin and of ncd suggest a structural rationale for the reversal of the direction of movement in chimeric kinesins.
authors.affiliation: Institut de Biologie Structurale (CEA/CNRS), Grenoble, 38027, France.
authors.forename: F
authors.initials: F
authors.lastname: Kozielski
authors.forename: S
authors.initials: S
authors.lastname: De Bonis
authors.forename: W P
authors.initials: WP
authors.lastname: Burmeister
authors.forename: C
authors.initials: C
authors.lastname: Cohen-Addad
authors.forename: R H
authors.initials: RH
authors.lastname: Wade
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D019281:Dimerization; D029721:Drosophila Proteins; D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D029721:Drosophila Proteins; C066696:ncd protein, Drosophila; D000244:Adenosine Diphosphate; D016547:Kinesins