title: Isolation of Orientia tsutsugamushi from patients in Shikoku and finding of a strain which grows preferentially at low temperatures.
abstract: Three strains of Orientia tsutsugamushi were isolated from patients in Anan City, Tokushima Prefecture. The strains were identified as Karp type by analyses of reactivities with type-specific monoclonal antibodies. One strain, Okazaki, was isolated in L cells cultivated at 31 C, but not in cells at 36 C or in mice. This strain showed better growth at 31 C than 36 C. This is the first report of an O. tsutsugamushi strain which grows preferentially at low temperatures.
authors.affiliation: Niigata College of Pharmacy, Department of Microbiology, Niigata, Japan. tamura@niigata-pharm.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Tamura
authors.forename: Y
authors.initials: Y
authors.lastname: Makisaka
authors.forename: T
authors.initials: T
authors.lastname: Enatsu
authors.forename: H
authors.initials: H
authors.lastname: Urakami
authors.forename: K
authors.initials: K
authors.lastname: Okubo
authors.forename: M
authors.initials: M
authors.lastname: Fukuhara
authors.forename: F
authors.initials: F
authors.lastname: Mahara
mesh_terms: D000368:Aged; D000818:Animals; D006801:Humans; D007564:Japan; D007739:L Cells; D008297:Male; D051379:Mice; D008875:Middle Aged; D012285:Orientia tsutsugamushi / Q000254:growth & development* / Q000302:isolation & purification*; D010537:Peritoneum / Q000382:microbiology; D012612:Scrub Typhus / Q000382:microbiology*; D013045:Species Specificity; D013154:Spleen / Q000382:microbiology; D013696:Temperature