Influence of peroperative lavage solutions on peritoneal defence mechanisms in vitro.
OBJECTIVE
To find out the in vitro reaction of mesothelial cells and polymorphonuclear leucocytes (PMN) to incubation with seven commonly-used lavage solutions.


METHODS
Experimental study.


METHODS
Laboratories, The Netherlands.


METHODS
Cultured human peritoneal mesothelial cells and isolated PMN.


METHODS
Incubation of cells with clinically used lavage solutions (sodium chloride, Hartmann's solution, povidone-iodine, Dakin's solution, taurolidine, chlorhexidine, and hydrogen peroxide).


METHODS
Activation of monolayers of mesothelial cells and PMN measured by release of oxygen free radicals (chemiluminescence) and interleukin (IL)-8 concentrations and toxic effects measured by morphology, release of lactate dehydrogenase, failure of the restriction of the passage of inulin, and incorporation of propidium iodide.


RESULTS
All solutions activated and killed mesothelial cells and PMN to some extent; the more concentrated the solution the greater the effect on these cells.


CONCLUSIONS
Lavage solutions both poison and stimulate mesothelial cells and neutrophils, and some solutions are more potent than others.
van Westreenen
M
M
Department of Surgery, Diakonessen Hospital, Amsterdam, The Netherlands.
Mul
F J
FJ
Pronk
A
A
Hoynck van Papendrecht
A A
AA
Diepersloot
R J
RJ
Roos
D
D
Leguit
P
P
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D004847:Epithelial Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007552:Isotonic Solutions / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010533:Peritoneal Lavage; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011206:Povidone-Iodine / Q000494:pharmacology; D016897:Respiratory Burst; D000077325:Ringer's Lactate; D012973:Sodium Hypochlorite / Q000494:pharmacology
D000891:Anti-Infective Agents, Local; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D011206:Povidone-Iodine; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite