Membrane chromatography: preparation and applications to protein separation.
As a result of the convective flow of solutes through porous membranes, membrane chromatography has a higher capture efficiency and a higher productivity than column chromatography and shows most promising industrial applications for the recovery, isolation, and purification of proteins and enzymes. This paper presents a comprehensive review of the methods for preparation of adsorptive membranes (such as surface modification, in situ copolymerization, direct formation from hydrophilic materials, and functionalized particulate-entrapped membranes) and deals particularly with novel macroporous chitin and chitosan membranes for protein separations developed by the authors.
Zeng
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X
Department of Chemical Engineering, State University of New York at Buffalo, Amherst, New York 14260, USA.
Ruckenstein
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E
D002240:Carbohydrate Sequence; D002482:Cellulose / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry; D048271:Chitosan; D002845:Chromatography / Q000379:methods*; D004798:Enzymes / Q000302:isolation & purification*; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D011506:Proteins / Q000302:isolation & purification*
D004798:Enzymes; D008567:Membranes, Artificial; D011506:Proteins; D002686:Chitin; D002482:Cellulose; D048271:Chitosan