Characterization of murine Th1 clones specific to egg antigen of Schistosoma japonicum and their interaction with cytokines.
T cell clones (B1, B21, B7, A25) specific to the soluble egg antigen (SEA) of Schistosoma japonicum were established from C3H/He mice immunized with SEA. These clones belonged to CD3+, CD4+ and CD8-Th1 cells, showing TCR-gamma delta-, TCR-alpha beta+ and Vbeta10b+. The molecular weights of target antigens recognized by the clones ranged from 51 to 80 kDa. Interleukin-2 (IL-2) and IL-12 could vigorously increase the proliferation response of the T clones to SEA; while IL-10 and transforming growth factor-beta1 (TGF-beta1) strongly inhibited the response. IL-12 activity was detected in the culture supernatant of T clones stimulated with SEA in the presence of APC (antigen presenting cells). This stimulation also upregulated the expression of the IL-12 receptor on the T clones. IL-12 from APC served as a costimulatory factor for the SEA induced proliferation of the T clone cells. Clone B1 was able to induce granuloma formation both in vivo and in vitro. These data provide further insight into the complicated interaction among SEA, T cell and cytokine at a clonal level in S. japonicum infection.
Yang
J Q
JQ
Department of Parasitology and Immunology, Yamanashi Medical University, Tamaho, Yamanashi 409-3898, Japan.
Tasaka
K
K
Chuang
C K
CK
Yoshikawa
H
H
Nakajima
Y
Y
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D006099:Granuloma / Q000469:parasitology / Q000473:pathology; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D018123:Receptors, Interleukin / Q000378:metabolism; D053707:Receptors, Interleukin-12; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D012549:Schistosoma japonicum / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D000947:Antigens, Helminth; D000954:Antigens, Surface; D016207:Cytokines; C508440:Il12rb1 protein, mouse; D007378:Interleukins; D015800:Protozoan Proteins; D011948:Receptors, Antigen, T-Cell; D018123:Receptors, Interleukin; D053707:Receptors, Interleukin-12; D015375:Receptors, Interleukin-2; D016212:Transforming Growth Factor beta