title: Isolation of Bacteroides fragilis mutants with in vivo growth defects by using Tn4400', a modified Tn4400 transposition system, and a new screening method.
abstract: A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich medium anaerobically.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
authors.forename: Y P
authors.initials: YP
authors.lastname: Tang
authors.forename: M H
authors.initials: MH
authors.lastname: Malamy
mesh_terms: D000818:Animals; D001441:Bacteroides fragilis / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D002460:Cell Line; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements