title: Mcl-1 and Bcl-xL are co-regulated by IL-6 in human myeloma cells.
abstract: Multiple myeloma (MM) is a slowly proliferative malignancy in which malignant plasma cells accumulate within the bone marrow. The expression of several anti-apoptotic proteins was evaluated by immunoblotting in human myeloma cell lines and in highly purified native myeloma cells. Expression of Bcl-xL, Mcl-1 and Bcl-2 was found in most of the samples; expression of Bcl-xL and Mcl-1 seemed to be related on myeloma cells. In a system of apoptosis by growth factor deprivation on myeloma cells, we showed that the effect of Bcl-2 seemed minimal whereas Mcl-1 and Bcl-xL were tightly regulated by interleukin (IL)-6. These findings underline the important role of Mcl-1 and Bcl-xL instead of Bcl-2 in IL-6-induced survival of myeloma cells.
authors.affiliation: Inserm U463, Institut de Biologie, Nantes, France.
authors.forename: D
authors.initials: D
authors.lastname: Puthier
authors.forename: S
authors.initials: S
authors.lastname: Derenne
authors.forename: S
authors.initials: S
authors.lastname: Barillé
authors.forename: P
authors.initials: P
authors.lastname: Moreau
authors.forename: J L
authors.initials: JL
authors.lastname: Harousseau
authors.forename: R
authors.initials: R
authors.lastname: Bataille
authors.forename: M
authors.initials: M
authors.lastname: Amiot
mesh_terms: D006801:Humans; D015151:Immunoblotting; D015850:Interleukin-6 / Q000494:pharmacology*; D009101:Multiple Myeloma / Q000378:metabolism*; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D051020:bcl-X Protein
chemical_list: C494811:BCL2L1 protein, human; D015850:Interleukin-6; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein