Targeted integration of a dominant neo(R) marker into a 2- to 3-Mb human minichromosome and transfer between cells.
The physical and genetic characterization of a stable human minichromosome in a Chinese hamster hybrid cell is described. The minichromosome is 2-3 Mb in size, is linear, and contains a complementing SDHC gene. It is derived from a human chromosome 1, including the centromere, some pericentric heterochromatin from 1q12, and 1-2 Mb of 1q21. Genomic DNA surrounding the SDHC gene was used to construct a targeting vector with a selectable drug resistance marker (neo(R)); the marker was then successfully integrated into the minichromosome. With the new selectable marker, the 8.2.3 minichromosome could be transferred into mouse LMTK(-) and 3T3 TK(-) cells.
Au
H C
HC
Department of Biology, University of California, San Diego, La Jolla. USA.
Mascarello
J T
JT
Scheffler
I E
IE
D000818:Animals; D016887:Cardiopulmonary Resuscitation; D002460:Cell Line; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002878:Chromosomes, Human, Pair 1; D006224:Cricetinae; D003412:Cricetulus; D016521:Electrophoresis, Gel, Pulsed-Field; D018014:Gene Transfer Techniques; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007739:L Cells; D051379:Mice; D013385:Succinate Dehydrogenase / Q000235:genetics*; D013937:Thymidine Kinase / Q000172:deficiency / Q000235:genetics
D013385:Succinate Dehydrogenase; D013937:Thymidine Kinase