title: Hepatocyte growth factor and invasion-stimulatory activity are induced in pleural fluid by surgery in lung cancer patients.
abstract: Hepatocyte growth factor (HGF) is a stromal cell-derived cytokine that can stimulate matrix invasion by carcinoma cells. We analysed the concentrations of HGF and invasion-stimulatory activity in pleural fluid after lung surgery. The concentration of HGF in pleural fluids was measured by enzyme-linked immunosorbent assay in seven patients who underwent pulmonary resection for primary or metastatic lung cancer. The effect of the pleural fluid on cancer cell invasion across reconstituted basement membrane (Matrigel) was assessed with a Boyden chamber assay using a lung adenocarcinoma cell line, A549. HGF levels in the pleural fluid after lung surgery ranged from 6.0 to 23.0 ng ml(-1) (average: 10.2 +/- 4.3 ng ml(-1)). The matrix invasion of lung carcinoma cells in the presence of the pleural fluid was significantly higher than that in the presence of culture medium alone or sera from normal subjects (P < 0.01). The invasion-stimulatory activity of the pleural fluid was strongly inhibited by HGF-neutralizing antibody. Positive correlation was found between the HGF level and invasion-stimulatory activity in the pleural fluids and normal sera (P = 0.0073). This is the first report demonstrating that the lung surgery induces a considerable amount of HGF, which is closely correlated with the invasion-stimulatory activity of the pleural fluid.
authors.affiliation: Department of Surgery I, Kyushu University Faculty of Medicine, Fukuoka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Uchiyama
authors.forename: T
authors.initials: T
authors.lastname: Morisaki
authors.forename: K
authors.initials: K
authors.lastname: Beppu
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Matsunari
authors.forename: A
authors.initials: A
authors.lastname: Nakatsuka
authors.forename: K
authors.initials: K
authors.lastname: Mizumoto
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D000368:Aged; D005260:Female; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010994:Pleura / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D017228:Hepatocyte Growth Factor