title: Transplantation of highly purified peripheral blood CD34+ cells from HLA-mismatched parental donors in 14 children: evaluation of early monitoring of engraftment.
abstract: HLA-mismatched family members may represent an important cell source for patients that require stem cell transplantation but lack both a matched sibling donor and a closely matched unrelated donor. We report the outcome of 19 transplantations from HLA two- or three- loci mismatched parental donors in which 14 pediatric patients with hematological malignancies or other disorders, received a median of 21.5 x 106 (range, 5.4-58) highly purified CD34+peripheral blood stem cells (PBSC), as well as 4.7 x 104 (range, 0.4-12) donor T cells per kg body weight. T cell depletion was performed using a two-step CD34-positive selection on two different magnetic beads devices. Ten of 14 patients presented with rapid myeloid engraftment. The four patients who presented with graft failure (two non-engraftments, two rejections) received a second stem cell graft and one a third. Graft rejection was detected early by polymerase chain reaction (PCR) analysis of FACS-sorted T cells. Eight of the 14 patients are still alive after a median observation period of 15. 6 months (range, 3-31.3) with full donor chimerism in all hematopoietic cell lineages. No acute organ graft-versus-host disease (GVHD) and no chronic GVHD have occurred. One patient experienced relapse of leukemia. We conclude that transplantation of allogeneic PBSC from haploidentical donors will open new perspectives for pediatric patients for whom an HLA-matched stem cell graft is not available. Close monitoring of recipient and donor hematopoiesis might be of clinical value, to recognize early engraftment or rejection.
authors.affiliation: St Anna Children's Hospital, Vienna, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Peters
authors.forename: S
authors.initials: S
authors.lastname: Matthes-Martin
authors.forename: G
authors.initials: G
authors.lastname: Fritsch
authors.forename: W
authors.initials: W
authors.lastname: Holter
authors.forename: T
authors.initials: T
authors.lastname: Lion
authors.forename: V
authors.initials: V
authors.lastname: Witt
authors.forename: P
authors.initials: P
authors.lastname: Höcker
authors.forename: G
authors.initials: G
authors.lastname: Fischer
authors.forename: K
authors.initials: K
authors.lastname: Dieckmann
authors.forename: R
authors.initials: R
authors.lastname: Handgretinger
authors.forename: T
authors.initials: T
authors.lastname: Klingebiel
authors.forename: H
authors.initials: H
authors.lastname: Gadner
mesh_terms: D000293:Adolescent; D018952:Antigens, CD34 / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D002678:Chimera; D006084:Graft Rejection; D006086:Graft vs Host Disease / Q000209:etiology; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000628:therapy*; D012008:Recurrence
chemical_list: D018952:Antigens, CD34