title: Isolation and characterization of viridin, a new 65 kDa antifungal protein from the mould Trichoderma viride.
abstract: A new extracellular antifungal protein with a yield of 10 mg per liter was isolated from the culture medium of the mould Trichoderma viride. The protein, which we named viridin, was purified by carboxymethyl-cellulose cation-exchange chromatography and Superose 12 HR 10/30 high-performance liquid chromatography. Viridin, a basic protein of approximately 65 kDa as determined by SDS-PAGE, inhibits the growth of the cotton pathogen Verticillum dahliae, the IC50 being 6 microM.
authors.affiliation: Laboratory of Plant Molecular Genetics, Shanghai Institute of Biochemistry, Academia Sinica, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Hao
authors.forename: C
authors.initials: C
authors.lastname: Geng
authors.forename: W
authors.initials: W
authors.lastname: Xie
authors.forename: Z
authors.initials: Z
authors.lastname: Gong
authors.forename: W Y
authors.initials: WY
authors.lastname: Liu
authors.forename: E
authors.initials: E
authors.lastname: Wang
mesh_terms: D000736:Androstenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003368:Gossypium / Q000382:microbiology; D008970:Molecular Weight; D014242:Trichoderma / Q000737:chemistry*; D020172:Verticillium / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000736:Androstenes; D000935:Antifungal Agents; D001430:Bacteriocins; C012097:viridin