[The study of quantitative karyotypic variability by induction of chromosomal instability in cultured cells of the Indian muntjac skin fibroblasts].
The influence of mycoplasmal contamination and somatic cell hybridization on the character of karyotypic variability in cell cultures of Indian muntjac skin fibroblasts has been investigated. Mycoplasma arginini and Acholeplasma laidlawii, used as factors inducing chromosomal instability, do not break the main regulations peculiar to intact control. They regulations are: 1) nonrandom character of cell distribution according to the number of chromosomal deviations from MSVK; 2) specific character of deviations of each chromosome from MSVK; 3) presence of significant connections between separate chromosomes by simultaneous mainly single directed numeral deviations. However, mycoplasmal contamination promotes the increase in the number of deviations in the direction of a decreasing chromosomes number. There is a breach of some connections between chromosomes by simultaneous deviations. They are chromosomes with broken connections according to the number of deviations which form telomeric associations (dicentrics). The number of these associations excel essentially intact control. The formation of new MSVK in subline M2 cells of the Indian muntjac in the process of chromosomal segregation in cell hybrid (M2 x clone of JF1 rat Jensen sarcoma) depends on the presence of significant connections between chromosomes by simultaneous numerical deviations in direction of MSVK formation. They are chromosomes that take part in the formation of new MSVK which form telomeric associations. These associations can be observed till stabilization of new MSVK. Probably, the support of the balance of karyotypic structure by factors inducing chromosomal instability is connected with change of some connections between chromosomes according to the number by simultaneous deviations as well as with the formation of dicentrics.
Polianskaia
G G
GG
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Samokish
V A
VA
D000127:Acholeplasma; D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005347:Fibroblasts; D014644:Genetic Variation; D007621:Karyotyping; D018668:Muntjacs / Q000235:genetics*; D009174:Mycoplasma
Issledovanie kolichestvennoĭ kariotipicheskoĭ izmenchivosti pri indu ktsii khromosomnoĭ nestabil'nosti v kul'tiviruemykh kletkakh fibroblastov kozhi indiĭskogo muntzhaka.