Essential roles in synaptic plasticity for synaptogyrin I and synaptophysin I.
We have generated mice lacking synaptogyrin I and synaptophysin I to explore the functions of these abundant tyrosine-phosphorylated proteins of synaptic vesicles. Single and double knockout mice were alive and fertile without significant morphological or biochemical changes. Electrophysiological recordings in the hippocampal CA1 region revealed that short-term and long-term synaptic plasticity were severely reduced in the synaptophysin/synaptogyrin double knockout mice. LTP was decreased independent of the induction protocol, suggesting that the defect in LTP was not caused by insufficient induction. Our data show that synaptogyrin I and synaptophysin I perform redundant and essential functions in synaptic plasticity without being required for neurotransmitter release itself.
Janz
R
R
Howard Hughes Medical Institute, The University of Texas Southwestern Medical School, Dallas 75235, USA.
Südhof
T C
TC
Hammer
R E
RE
Unni
V
V
Siegelbaum
S A
SA
Bolshakov
V Y
VY
D000818:Animals; D001921:Brain / Q000473:pathology; D004558:Electric Stimulation; D017774:Long-Term Potentiation / Q000502:physiology; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D010375:Pedigree; D013569:Synapses / Q000502:physiology*; D062805:Synaptogyrins; D016708:Synaptophysin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D013997:Time Factors
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D062805:Synaptogyrins; D016708:Synaptophysin