title: Duration-dependent effect of transient neonatal hypothyroidism on sertoli and germ cell number, and plasma and testicular interstitial fluid androgen binding protein concentration.
abstract: The impact of transient neonatal hypothyroidism on growth and function of puberal testis during different milestones of postnatal testicular development was studied in Wistar rats. Rat pups were made hypothyroid for 10, 15, 30, 40 and 60 days of postnatal age from birth by providing 0.05% (W/V) methimazole (MMI) in the drinking water of the mother, from day 1 postpartum till weaning (25 days postpartum) and thereafter in the drinking water. Control rats were raised without MMI treatment. Sertoli cell number and its function was assessed on day 60 postpartum. Sertoli cell number increased consistently in 10, 15, 30 and 40 days transient hypothyroid rats but decreased in rats subjected to continuous hypothyroidism from birth to 60 days postpartum. Rats subjected to continuous hypothyroidism from birth showed spermatogenic arrest at puberty and had only a single layer of spermatogonia. Transient neonatal hypothyroidism for 10 (or) 15 days from birth increased spermatocytes (pachytene and zygotene), spermatids (elongated and round) whereas, that of 30 and 40 days decreases the number of germ cells. Plasma androgen binding protein (ABP) concentration decreased in puberal rats belonging to all groups, whereas the testicular interstitial fluid (TIF) concentration of ABP increased significantly in 10 and 15 days hypothyroid rats while it decreased in all other groups. These findings indicate that the mitogenic activity of Sertoli cell is increased irrespective of the duration of transient neonatal hypothyroidism. However, the functional activity of Sertoli cells (ABP production) in these puberal rats varies depending upon the postnatal period at which the animals were in hypothyroid state.
authors.affiliation: Department of Endocrinology, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, India. rmanimaran@hotmail.com
authors.forename: R R
authors.initials: RR
authors.lastname: Maran
authors.forename: R
authors.initials: R
authors.lastname: Sivakumar
authors.forename: J
authors.initials: J
authors.lastname: Arunakaran
authors.forename: B
authors.initials: B
authors.lastname: Ravisankar
authors.forename: K
authors.initials: K
authors.lastname: Ravichandran
authors.forename: V
authors.initials: V
authors.lastname: Sidharthan
authors.forename: D A
authors.initials: DA
authors.lastname: Jeyaraj
authors.forename: M M
authors.initials: MM
authors.lastname: Aruldhas
mesh_terms: D000375:Aging; D000727:Androgen-Binding Protein / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D005110:Extracellular Space / Q000378:metabolism; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008713:Methimazole; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000473:pathology; D012708:Sertoli Cells / Q000473:pathology*; D013076:Sperm Count; D013087:Spermatids; D013090:Spermatocytes; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D013963:Thyroid Hormones / Q000097:blood; D013997:Time Factors
chemical_list: D000727:Androgen-Binding Protein; D013963:Thyroid Hormones; D008713:Methimazole