[Functional morphology of nuclear organizer regions of chromosomes and of nucleoli in cells of human multiple myeloma cell lines. II.Relationship between immunoglobulin E production and argentophilia of nuclear organizer regions of chromosomes in U 266 cell line].
The IgE content in both cytoplasm and culture medium of U 266 myeloma cells, was studied by the enzyme-linked immunoassay in correlation with Ag-staining of NORs of chromosomes in their nuclei throughout 9 days after cell seeding. Proliferative activity of the cells was evaluated with 3H-thymidine labeling. The average values of both the cytoplasmic IgE content and its secretion level in U 266 cell population, being in logarithmic growth phase, were higher in S-phase cells as compared with G1-cells. On comparison of results of the present and previous (Turilova et al., 1998) studies it was revealed that U 266 myeloma cell line had a high stability of cell proliferation kinetics and IgE secretion and the dynamics of Ag-NORs-staining, while the number of argentophilic grains in the cell nuclei in the present experiment was higher to correlate with an enhanced IgE production. It is suggested that Ag-NORs-staining reflects the level of specific functional activity of cells in the U 266 line.
Turilova
V I
VI
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Smirnova
T D
TD
Samoĭlovich
M P
MP
Sukhikh
T R
TR
D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000378:metabolism* / Q000648:ultrastructure*; D016622:Silver Staining; D014407:Tumor Cells, Cultured
D007073:Immunoglobulin E
Funktsional'naia morfologiia iadryshkoobrazuiushchikh raĭonov khromosom i iadryshek v kletkakh liniĭ mnozhestvennoĭ mielomy cheloveka.II. Vzaimootnoshenie produktsii immunoglobulina E i argentof il'nosti iadryshkoobrazuiushchikh raĭonov khromosom v kletkakh linii U 266.