Structural definition of arabinomannans from Mycobacterium bovis BCG.
The structures of the hydrophilic parietal and cellular arabinomannans isolated from Mycobacterium bovis BCG cell wall [Nigou et al. (1997) J Biol Chem 272: 23094-103] were investigated. Their molecular mass as determined by MALDI-TOF mass spectrometry was around 16 kDa. Concerning cap structure, capillary electrophoresis analysis demonstrated that dimannoside (Manpalpha1-->2Manp) was the most abundant motif (65-75%). Using two-dimensional 1H-13C NMR spectroscopy, the mannan core was unambiguously demonstrated to be composed of -->6Manpalpha1--> backbone substituted at some O-2 by a single Manp unit. The branching degree was determined as 84%. Finally, arabinomannans were found to be devoid of the phosphatidyl-myo-inositol anchor and, by aminonaphthalene disulfonate tagging, the mannan core was shown to contain a reducing end. This constitutes the main difference between arabinomannans and lipoarabinomannans from Mycobacterium bovis BCG.
Nigou
J
J
Institut de Pharmacologie et de Biologie Structurale du Centre National de la Recherche Scientifique, UPR 9062, Toulouse, France.
Gilleron
M
M
Brando
T
T
Vercellone
A
A
Puzo
G
G
D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008351:Mannans; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C012189:arabinomannan; D008358:Mannose