title: B1 integrin activation inhibits in vitro tube formation: effects on cell migration, vacuole coalescence and lumen formation.
abstract: Human endothelial cells (EC), when plated onto gels of extracellular matrix proteins such as Matrigel or collagen form capillary tubes in a process thought to mimic angiogenesis. We have shown previously that the extent of tube formation and the phenotype of the lumen are regulated by integrins (Gamble et al 1993) and lumen formation occurs through a process of vacuolization, coalescence and ultimate directional fusion of these vacuoles with the plasma membrane (Meyer 1997 et al). We now show here that activation of beta1 integrins on endothelial cells inhibits tube formation. On collagen gels, endothelial cells treated with 31 activating antibody 8A2 failed to migrate into the gel and tube formation was inhibited. Although several integrins mediate EC attachment to collagen alpha2beta1 is the chief determinant of EC behaviour since a blocking antibody to (alpha2beta1 reversed the effect of 8A2. On Matrigel tube formation was also inhibited by 8A2 treatment although cell alignment and sprout formation was still evident. Electron microscopy revealed the organisation of normal numbers of cells into solid sprouts and the formation of small intracellular vacuoles suggesting that initial stages of tube formation including cell migration were unaffected. However, beta1 integrin activation inhibited the coalescence of these small vacuoles into larger vacuoles, the recruitment of more cells into the sprout and the subsequent formation of mature lumen. The inhibition of capillary tube formation by beta1 activation was time dependent and long lasting. The critical time for activation of the beta1 integrin was the initial 1-2h after plating in order to inhibit tube formation although once activated, the beta1 mediated inhibition on Matrigel was still evident 4 days later. Our results suggest that beta1 integrins are critical in capillary tube formation in at least two phases. beta1 integrins are essential for migration of EC through collagen gels. Independently, beta1 integrins, although not involved in initial vacuole formation, are involved in the process of vacuole coalescence and subsequent lumen formation since beta1 integrin activation inhibits these processes.
authors.affiliation: Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science and University of Adelaide Frome Road, South Australia. Jennifer.Gamble@imvs.sa.gov.au
authors.forename: J
authors.initials: J
authors.lastname: Gamble
authors.forename: G
authors.initials: G
authors.lastname: Meyer
authors.forename: L
authors.initials: L
authors.lastname: Noack
authors.forename: J
authors.initials: J
authors.lastname: Furze
authors.forename: L
authors.initials: L
authors.lastname: Matthias
authors.forename: N
authors.initials: N
authors.lastname: Kovach
authors.forename: J
authors.initials: J
authors.lastname: Harlant
authors.forename: M
authors.initials: M
authors.lastname: Vadas
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology; D001667:Binding, Competitive; D002196:Capillaries / Q000187:drug effects* / Q000254:growth & development*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D039421:Integrin alpha2; D019012:Integrin beta1 / Q000276:immunology / Q000494:pharmacology* / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D014470:Umbilical Cord / Q000166:cytology; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039421:Integrin alpha2; D019012:Integrin beta1; D003094:Collagen