title: Bioassay-directed chemical analysis and detection of mutagenicity in ambient air of the coke oven.
abstract: In the present study, we summarize the results of studies on the mutagenic potential of the main fractions and subfractions of extractable organic material (EOM) in the ambient air at the workplaces of the coke oven. The objective of our experiments was to apply the Bioassay-Directed Chemical Analysis (with the use of the Ames test) for the identification of the differences in the mutagenicity of these fractions, in relationship to the complex mixture of EOM in occupational air. From the evaluation of results, it is possible to deduce the following conclusions: (1) The comparison of the mutagenicity in the main fractions (basic, acidic, neutral) demonstrates the existence of differences in mutagenic potential. Of the total mutagenicity, 20.4% is in the basic fraction, 25.4% in the acidic fraction and 54.2% in the neutral fraction. (2) In general, 90.1% of the mutagenicity found in the basic, acidic and neutral fractions together was associated with the requirement of metabolic activation in vitro (+S9). In the case of the neutral fraction, it was 51.8%. (3) These results also suggest that frameshift mutations are the major component (53.8%) of the total mutagenicity of the main fractions. (4) With regards to the mutagenicity of organic compounds in the neutral fraction it appeared that genotoxicants of its subfractions (slightly and moderately polar and aromatic) play the main role. Carcinogenic aromatic hydrocarbons (PAH) and genotoxic nitrocompounds play an important role as determinants of the mutagenic potential of complex mixtures of harmful compounds in ambient air. This is confirmed first by the results of short-term bacterial tests.
authors.affiliation: Department of Genetic Toxicology, Regional Institute of Hygiene, Ostrava, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Dobiás
authors.forename: J
authors.initials: J
authors.lastname: Kůsová
authors.forename: O
authors.initials: O
authors.lastname: Gajdos
authors.forename: P
authors.initials: P
authors.lastname: Vidová
authors.forename: D
authors.initials: D
authors.lastname: Gajdosová
authors.forename: J
authors.initials: J
authors.lastname: Havránková
authors.forename: M
authors.initials: M
authors.lastname: Fried
authors.forename: B
authors.initials: B
authors.lastname: Binková
authors.forename: J
authors.initials: J
authors.lastname: Topinka
mesh_terms: D000395:Air Pollutants, Occupational / Q000009:adverse effects* / Q000032:analysis*; D000818:Animals; D001681:Biological Assay; D001711:Biotransformation; D005591:Chemical Fractionation; D002849:Chromatography, Gas; D003077:Coke; D004784:Environmental Monitoring / Q000379:methods*; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000009:adverse effects* / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects* / Q000032:analysis*; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D000395:Air Pollutants, Occupational; D003077:Coke; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons