title: Cleavage specificity of a myofibril-bound serine proteinase from carp (Cyprinus carpio) muscle.
abstract: Previously, we reported the purification and characterization of a myofibril-bound serine proteinase (MBP) from carp muscle (Osatomi K, Sasai H, Cao M-J, Hara K, Ishihara T. Comp Biochem Physiol 1997;116B:159-66). In the present study, the N-terminal amino acid sequence of the enzyme was determined, which showed high identity with those of other trypsin-like serine proteases. The cleavage specificity of MBP for dibasic and monobasic residues was investigated using various fluorogenic substrates and peptides. Analyses of the cleaved peptide products showed that the enzyme hydrolyzed peptides both at monobasic and dibasic amino acid residues. Monobasic amino acid residues were hydrolyzed at the carboxyl side; dibasic residues were cleaved either at the carboxyl side of the pair or between the two basic residues and the enzyme showed a cleavage preference for the Arg-Arg pair. Unexpectedly, MBP hydrolyzed lysyl-bradykinin and methionyl-lysyl-bradykinin at the carboxyl side of Gly fairly specifically and efficiently displaying a unique cleavage. Because MBP also degraded protein substrates such as casein and myofibrillar proteins, the substrate specificity of MBP appeared not to be strictly specific.
authors.affiliation: Graduate School of Marine Science and Engineering, Nagasaki University, Japan.
authors.forename: M J
authors.initials: MJ
authors.lastname: Cao
authors.forename: K
authors.initials: K
authors.lastname: Osatomi
authors.forename: H
authors.initials: H
authors.lastname: Pangkey
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Ishihara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002122:Calcium Chloride; D002347:Carps; D004285:Dogs; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009210:Myofibrils / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D012697:Serine Endopeptidases; D002122:Calcium Chloride