Specificities of multiple sclerosis cerebrospinal fluid and serum antibodies against mimotopes.
In order to characterize antigenic epitopes specifically targeted by the immune response of patients with multiple sclerosis (MS), the antibody specificities of cerebrospinal fluids (CSF) and sera from the same MS patients have been analyzed using a random pentadecapeptide library displayed on phage. The 3 peptides (mimotopes) selected with MS sera were not disease-specific. In contrast, the combination of 4 MS CSF selected mimotopes, allowed the detection of specific antibodies in 21 of 60 MS CSF whereas only 2 of 27 CSF from patients with other neurological diseases equally recognized the 4 mimotopes. Some amino acid similarities were found between two MS CSF selected mimotopes and two envelope regions (319-329 and 433-443, respectively) of MSRV (multiple-sclerosis-associated retrovirus) and the related endogenous retrovirus HERV-W.
Jolivet-Reynaud
C
C
Unité Mixte de Recherche 103, Centre National de la Recherche Scientifique-bioMérieux, 46 Allée d'Italie, Lyon Cedex 07, 69364, France. Colette.Jolivet@ens-bma.cnrs.fr
Perron
H
H
Ferrante
P
P
Becquart
L
L
Dalbon
P
P
Mandrand
B
B
D000595:Amino Acid Sequence; D000906:Antibodies / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity / Q000276:immunology; D001435:Bacteriophages / Q000276:immunology; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D018716:Molecular Mimicry; D009103:Multiple Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D009842:Oligopeptides / Q000276:immunology
D000906:Antibodies; D000939:Epitopes; D009842:Oligopeptides