Detection of antibodies against Campylobacter jejuni serogroup PEN O:19 purified flagellar protein in a patient with Guillain-Barré syndrome.
C. jejuni serogroup PEN O:19 was isolated from a stool specimen from a patient with Guillain-Barré syndrome (GBS). Flagellar protein was isolated and purified from reference strain C. jejuni PEN O:19, ATCC 43,446, as well as from a homologous patient strain. Antibodies against flagellar protein were detected by means of immunoblotting, enzyme-linked immunosorbent assay (ELISA) and tube agglutination test. The antibody titres were found to be directly correlated at the beginning and in the recovery phase of GBS. Antibodies of IgG and IgA classes were present from the very onset of the disease as well as 5 months later, but with a lower titre population. However, antibodies of the IgM class were persistent only at the onset of the infection and disappeared during the following 5 months. Our results strongly support the hypothesis that in GBS patients, antiflagellar antibodies are induced during C. jejuni infection and can be used in the diagnosis of C. jejuni-associated GBS.
Lange
D
D
Johnson & Johnson Medical, Norderstedt, Germany. dlange@medde.jnj.com
Aleksic
S
S
Kassubek
J
J
Vrvic
M M
MM
Kist
M
M
Steinbrückner
B
B
Mitova
M
M
D000328:Adult; D000371:Agglutination; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D016123:Campylobacter jejuni / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005408:Flagellin / Q000276:immunology* / Q000302:isolation & purification; D020275:Guillain-Barre Syndrome / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D013997:Time Factors
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005408:Flagellin