title: An overall perspective of X-ray damage to a DNA oligomer mediated by reactive oxygen species.
abstract: The products produced by X irradiation of an oxygenated aqueous solution containing d(CpApTpG) were analyzed by NMR spectroscopy and mass spectrometry. Thirteen different base modifications were detected, including a novel product formed by the addition of oxygen to guanine. Seven different strand break products were identified, including strands having 5'-phosphoryl groups, 3'-phosphoryl groups and groups having 3'-phosphoglycolates as termini. The products produced in largest yield contained base modifications: Pyrimidine bases degraded to a formamido moiety, the 8-oxo-7,8-dihydroguanine (8-oxoguanine) lesion, and double base lesions in which both the 8-oxo-7,8-dihydroguanine lesion and a formamido remnant are present.
authors.affiliation: Biophysics Department, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Box
authors.forename: J B
authors.initials: JB
authors.lastname: Dawidzik
authors.forename: J C
authors.initials: JC
authors.lastname: Wallace
authors.forename: C R
authors.initials: CR
authors.lastname: Hauer
authors.forename: R F
authors.initials: RF
authors.lastname: Stack
authors.forename: M J
authors.initials: MJ
authors.lastname: Rajecki
authors.forename: E E
authors.initials: EE
authors.lastname: Budzinski
mesh_terms: D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species; D012996:Solutions; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014965:X-Rays
chemical_list: D009838:Oligodeoxyribonucleotides; D017382:Reactive Oxygen Species; D012996:Solutions; D004247:DNA