title: Expression of protein tyrosine phosphatase PTP-RL10 and its isoform in the mouse testis.
abstract: BACKGROUND
  The cytoplasmic-type protein tyrosine phosphatase PTP-RL10/PTPD1/PTP2E contains an ezrin-like domain and associates with the c-Src protein tyrosine kinase. Because tyrosine phosphorylation regulated by protein tyrosine kinases and phosphatases is involved in activation, migration, differentiation and proliferation of various cell types, the expression of PTP-RL10 and c-src in the mouse testis was investigated.
  
  
  METHODS
  Testes of wild-type mice and W/W(v) mutant mice that lack germ cells were analyzed by northern blotting, in situ hybridization histochemistry and reverse transcriptase-polymerase chain reaction for the expression of PTP-RL10, its isoform PTP-RL10b and c-src. Expression in the Sertoli cell lines, TM4 and TAMA26 was also analyzed.
  
  
  RESULTS
  PTP-RL10 transcripts of 5.7kb and 2.9kb in size were detected in the testis. In situ hybridization histochemistry demonstrated that the 5.7kb transcripts were expressed in pachytene spermatocytes and somatic cells including Sertoli cells, in which c-src transcripts were detected. The 2.9kb transcript encoded a novel isoform, PTP-RL10b, that has the catalytic domain but not the domains that associate with c-Src. PTP-RL10b was expressed mainly in the testicular somatic cells. TM4 and TAMA26 cells were found to co-express PTP-RL10, PTP-RL10b and c-src transcripts.
  
  
  CONCLUSIONS
  PTP-RL10 and its isoform are expressed in the Sertoli cells and are suggested to play roles in spermatogenesis by interacting with c-Src and/or other protein(s).
authors.affiliation: Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tokuchi
authors.forename: H
authors.initials: H
authors.lastname: Higashitsuji
authors.forename: H
authors.initials: H
authors.lastname: Nishiyama
authors.forename: K
authors.initials: K
authors.lastname: Nonoguchi
authors.forename: T
authors.initials: T
authors.lastname: Nagao
authors.forename: J H
authors.initials: JH
authors.lastname: Xue
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: O
authors.initials: O
authors.lastname: Ogawa
authors.forename: J
authors.initials: J
authors.lastname: Fujita
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015870:Gene Expression; D005854:Germ Cells; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D020033:Protein Isoforms; D017027:Protein Tyrosine Phosphatases / Q000032:analysis*; D012708:Sertoli Cells; D013091:Spermatogenesis; D013737:Testis / Q000201:enzymology*
chemical_list: D020033:Protein Isoforms; D017027:Protein Tyrosine Phosphatases