Effect of interleukin-1 beta on cardiac hypertrophy and production of natriuretic peptides in rat cardiocyte culture.
This study was designed to examine the effects of interleukin-1 beta (IL-1 beta) on myocyte (MC) hypertrophy and the production of A-type natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) in rat ventricular cardiocyte culture, and to investigate the role of nonmyocyte (NMC) in this process. We examined the effects of IL-1 beta on the production of ANP and BNP in comparison with the effects of endothelin-1 (ET-1) by using two types of neonatal rat cardiocyte culture; MC-enriched culture and MC-NMC coculture. In the MC-enriched culture, the increase in secretion of ANP and BNP was small in treatment with IL-1 beta (1000 pg/ml), while ET-1 (10 nM) markedly augmented the secretion of ANP and BNP. In the MC-NMC coculture, IL-1 beta and ET-1 each significantly augmented the secretion of ANP and BNP. The degree of the increase of ANP and BNP was equivalent between IL-1 beta and ET-1. As for the morphological changes of MCs, IL-1 beta induced the star-shaped MC hypertrophy characterized by elongation and pointed edges only in the MC-NMC coculture, while ET-1 induced the MC hypertrophy characterized by shapes of squares, triangles or circles in both cultures. This study shows that IL-1 beta induces unique cardiac hypertrophy and the marked secretion of ANP and BNP, and that NMC is indispensable when treated with IL-1 beta.
Harada
E
E
Department of Cardiovascular Medicine, Kumamoto University School of Medicine, Japan.
Nakagawa
O
O
Yoshimura
M
M
Harada
M
M
Nakagawa
M
M
Mizuno
Y
Y
Shimasaki
Y
Y
Nakayama
M
M
Yasue
H
H
Kuwahara
K
K
Saito
Y
Y
Nakao
K
K
D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics*; D006332:Cardiomegaly; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006352:Heart Ventricles; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain; D051381:Rats; D017208:Rats, Wistar
D019332:Endothelin-1; D007375:Interleukin-1; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor