An in situ hybridization screen for the rapid isolation of differentially expressed genes.
To rapidly isolate genes specifically expressed during medaka development we generated a cDNA library enriched for genes expressed in the head region of the developing embryo. Clones were spotted on filters automatically and preselected for abundantly expressed genes by hybridizing them with a probe derived from RNA of undifferentiated totipotent cells. Of the nonhybridizing clones 153 were chosen randomly and further analyzed by whole-mount in situ hybridization. There were 67 selected clones differentially expressed in the developing embryos, and 48 of these were expressed in the developing head. Differentially expressed genes were either of novel type or showed homology to known genes containing DNA binding motifs or to putative housekeeping genes.
Henrich
T
T
Max Planck Institute for Biophysical Chemistry, Am Fassberg, D-37077 Göttingen, Germany.
Wittbrodt
J
J
D000818:Animals; D000943:Antigens, Differentiation; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D017403:In Situ Hybridization / Q000379:methods*; D009990:Oryzias / Q000235:genetics
D000943:Antigens, Differentiation; D018076:DNA, Complementary