title: Effects of oestradiol-17beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail.
abstract: In order to study the effects of steroid hormones on steroidogenesis in the avian ovary, quail granulosa cells were cultured with follicle stimulating hormone (FSH), oestradiol-17beta or testosterone. The progesterone content of the medium during the culture period of 66 h and the following 3 h of incubation with luteinising hormone (LH), was measured by radioimmunoassay. When FSH, oestradiol-17beta or testosterone were added during the 66 h culture, subsequent progesterone production by the cells during 3 h of incubation with LH was significantly increased. However, testosterone also stimulated progesterone production in the medium during the 66 h culture period, whereas FSH oroestradiol-17beta did not. Addition of staurosporine during culture inhibited both LH-stimulated progesterone production and testosterone-stimulated progesterone production. These results indicate that the processes during which granulosa cells acquired responsiveness to LH, and testosterone stimulates progesterone production might both be mediated by a staurosporine-sensitive protein kinase C-dependent pathway in quail granulosa cells.
authors.affiliation: Shizuoka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasanami
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003370:Coturnix; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*; D019311:Staurosporine / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D019311:Staurosporine