title: Optical measurements of synchronized activity in isolated mammalian cerebellum.
abstract: An experimental system that combines optical imaging of voltage-sensitive dyes with an in vitro isolated cerebellum preparation is described. The optical imaging system is based on a photodiode array and two rather simple amplification stages. The isolated cerebellum preparation preserves the integrity of the neuronal circuitry, thus allowing the exploration of the path of information flow. In this study, we characterize the nature and sources of the optical signal evoked in the cerebellar cortex by surface stimulation. We show that this signal reflects inhibitory and excitatory synaptic potentials generated by cell bodies and dendrites of cortical neurons, whereas action potentials of the parallel fibers are not detected by the system. The spatial distribution of the optical signals agrees with the classical view of cerebellar activity following surface stimulation. Hence, this experimental system provides a powerful means to explore the functional organization, in time and space, of the cerebellar cortex.
authors.affiliation: Department of Neurobiology, Life Sciences Institute and Center for Neural Computation, The Hebrew University, Jerusalem, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Cohen
authors.forename: Y
authors.initials: Y
authors.lastname: Yarom
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002525:Cerebellar Cortex / Q000166:cytology / Q000502:physiology; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D002536:Cerebral Arteries; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D011726:Pyridinium Compounds; D011810:Quinoxalines / Q000494:pharmacology; D011930:Reaction Time; D013343:Styrenes; D013569:Synapses / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D011810:Quinoxalines; C096453:RH 795; D013343:Styrenes; D013779:Tetrodotoxin; C056723:FG 9041; C054158:RH 414; D001640:Bicuculline