title: Effect of active molluscicidal component of spices on different enzyme activities and biogenic amine levels in the nervous tissue of Lymnaea acuminata.
abstract: In vivo exposure of Lymnaea acuminata to thymol and [6]-gingerol (active molluscicidal components of Trachyspermum ammi and Zingiber officinale, respectively) indicates that they significantly alter acetylcholinesterase, lactic dehydrogenase, succinic dehydrogenase and cyto-oxidase activity in the nervous -tissue of snails. In vitro exposure showed that, except for acetylcholinesterase and lactic dehydrogenase, no significant changes were observed in cyto-oxidase and succinic dehydrogenase activity in the nervous tissue of L. acuminata. Sublethal exposure to thymol and [6]-gingerol reduced the levels of 5-hydroxytryptamine (5-HT) and dopamine (DA) in the nervous tissue of L. acuminata. There was, however, no significant change in the level of 5-hydroxy indol acetic acid (5-HIAA). Thymol and [6]-gingerol thus affects all the known neurotransmission mechanisms in the snail either separately or through a complex interaction between the different neurotransmitters. This may account for their toxicity to snails.
authors.affiliation: Department of Zoology, DDU Gorakhpur University, Gorakhpur PIN-273 009, U.P., India.
authors.forename: V K
authors.initials: VK
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: D K
authors.initials: DK
authors.lastname: Singh
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D002396:Catechols; D004298:Dopamine / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005233:Fatty Alcohols / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008975:Molluscacides / Q000494:pharmacology*; D009417:Nerve Tissue / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D012908:Snails / Q000201:enzymology*; D017365:Spices; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013943:Thymol / Q000494:pharmacology*
chemical_list: D001679:Biogenic Amines; D002396:Catechols; D005233:Fatty Alcohols; D008975:Molluscacides; D010936:Plant Extracts; D012701:Serotonin; D013943:Thymol; D006897:Hydroxyindoleacetic Acid; C007845:gingerol; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000110:Acetylcholinesterase; D004298:Dopamine